LOCUS       DASWGG010000001         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_322487, whole genome shotgun sequence.
ACCESSION   DASWGG010000001 DASWGG010000000
VERSION     DASWGG010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322487"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VF954_00005"
     CDS             complement(<1..376)
                     /locus_tag="VF954_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9529485.1"
                     /translation="MTWKRPVPLVGDTSTAAERRREPQAWAFPLDARAHLYQLIAARR
                     DIRRFRPDPIEAETLHRVLAAAHAAPSVGLSQPWRFVVVRDGSTRDEAAWMADQQRQL
                     QAAQLEPDAARHLLDLQLEGIRE"
     gene            471..902
                     /locus_tag="VF954_00010"
     CDS             471..902
                     /locus_tag="VF954_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9529486.1"
                     /translation="MTSEPRARWAAALALVLAVTGCAREHRVEAGATSASTGGAASTV
                     AATGAVGYVTAGPTCPVERAGHPCPPSPVNARVEAADSTGRVVAASNTDASGRYSLSL
                     RPGSYTLTATPPNPFPRCRPAPVTVADGPTRADIGCDSGIR"
     gene            951..1199
                     /locus_tag="VF954_00015"
     CDS             951..1199
                     /locus_tag="VF954_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529487.1"
                     /translation="MVDKELAGRLIKALSMLDEVAGEVTADDAVAELDEATLQVFWRD
                     WPNISSWAGALWRKLQADLEHPARPAQDSDLDEVGGSG"
     gene            1209..1592
                     /locus_tag="VF954_00020"
     CDS             1209..1592
                     /locus_tag="VF954_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9529488.1"
                     /translation="MARLGDIMTRAVFAVAPETPVAEVARSMVKGRFGSAVVTRSELL
                     VGIFTERDVLRAAASGDDLSTSAVSRWMTADPESVGPDVDSEAAAEVMLSRGFRHLPV
                     LDGNRLLGIVSLRDVLATRIRGGGK"
     gene            1684..2121
                     /locus_tag="VF954_00025"
     CDS             1684..2121
                     /locus_tag="VF954_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529489.1"
                     /translation="MSDSLGNARERRVGLRTAMGEVEASLASPATGRKGPWLRAVRED
                     MQTLSAALELHISTTEAPGGLLDDIAATAPDLADRVKQTRLDHERLGYRLHEALQAIS
                     APEVDVADVRARVVELLAAIVHHRHEGADLVYEAFDVDIDSAD"
BASE COUNT          295 a          820 c          881 g          295 t
ORIGIN      
        1 cttcgcggat gccctcgagc tggaggtcga ggaggtggcg ggccgcgtcc ggctccagct
       61 gggcggcctg gagctggcgc tgctggtcgg ccatccacgc ggcctcgtcc cgggtcgagc
      121 cgtcgcggac caccacgaac cgccagggct gcgacagccc cacgctgggc gcggcgtgcg
      181 ccgccgccag gacccggtgc agggtctcgg cctcgatcgg gtcgggccgg aaccgccgga
      241 tgtcgcggcg ggccgcgatc agctggtaga gatgcgcccg ggcgtcgagc gggaaggccc
      301 acgcctgcgg ttcgcgccgc cgctcggccg ccgtcgaggt gtcgcccacg aggggcacgg
      361 gccgcttcca cgtcatggcc cgacgagact acgcgcccgg gcccggaacg cccgggcccg
      421 gaacgcccgg cgccgggaac tccccggggt ggcgctaccg tcctatcccg gtgacgagcg
      481 aaccccgggc ccggtgggcg gccgccctcg ccctggtgct ggcggtgaca ggctgcgccc
      541 gcgaacaccg ggtggaggcc ggggcgacgt cggcctccac cggaggtgcc gcgtcgacgg
      601 tggcggccac cggagccgtc ggctacgtca ccgccgggcc gacgtgcccg gtcgagcgcg
      661 ccggccatcc ctgcccgccc agcccggtga acgcccgcgt ggaggccgcc gactcgacgg
      721 gccgggtcgt ggcggcctcc aacaccgatg cctcgggccg gtactcgctg agcctgcgcc
      781 ccggctcgta caccctcacg gccaccccgc ccaacccgtt cccgcgctgc cggccggcgc
      841 ctgtcacggt ggccgacgga ccgacgcggg cggacatcgg ttgcgactcc ggcatccgtt
      901 gaggggtcgc cacacgcgac gggttcccgg acgcgcgcta gcgttcgcgt gtggtcgaca
      961 aggagctcgc cggacggttg atcaaggccc tgtcgatgct cgacgaggtg gccggcgagg
     1021 tcacggccga cgacgcggtc gccgagctgg acgaggcgac cctccaggtc ttctggcggg
     1081 actggcccaa catcagctcg tgggcaggcg ccctgtggcg caagctccag gcggacctgg
     1141 agcatcccgc ccgcccggct caggactccg acctcgacga ggtcgggggc agcggctgag
     1201 gcgcgcccgt ggcccggctc ggcgacatca tgacgagagc ggtcttcgcg gtggcgcctg
     1261 agacgccggt cgccgaggtg gccaggagca tggtcaaagg ccggttcggc tccgcggtcg
     1321 tgacgaggag cgagttgctc gtgggcatct tcaccgaacg cgacgtcctt cgggctgcgg
     1381 cctcgggcga cgacctgtcg acctcggcgg tgtcgcggtg gatgacggcc gacccggagt
     1441 cggtggggcc ggacgtcgac tcggaagcgg cggccgaggt catgttgagc agggggttcc
     1501 ggcacctccc ggtgctcgac ggcaaccggc tgctgggcat cgtcagcctt cgcgacgtgc
     1561 tcgccacgcg catccgcggt ggcgggaagt gatgcgctgg gccgatcggg ccgggcgggc
     1621 gaggcccgtt cgccgccgcc ccggctgcag cgcctggccg gtagcctacg gcggcaggtc
     1681 cggatgagcg actcgctggg gaacgcccgg gagcgccggg tcggtctgcg cacggccatg
     1741 ggcgaggtcg aggcctcgct ggcgagcccg gcgaccggtc ggaagggccc gtggctgcgc
     1801 gccgtgcgcg aggacatgca gaccctgtcc gccgccctcg agctccacat ctcgacgacg
     1861 gaggctccgg gcggcttgct ggacgatata gcggccaccg cgcccgacct cgccgatcgg
     1921 gtgaagcaga cgagattgga ccacgagcgc ctgggctacc ggctgcacga ggcgttgcag
     1981 gccatctcgg cccctgaggt cgacgtggcc gatgtccggg cgcgcgtggt cgagctcttg
     2041 gccgcgatcg tgcaccaccg ccacgagggc gcggacctcg tctacgaggc cttcgacgtg
     2101 gacatcgaca gcgccgactg acgcacgcgc cccgagcagg aggttgtcgg gctcacggtt
     2161 ggtggagcgc gacgccgatg acgtttgcga ggctctgcat gaaatgagcg gcggtggggc
     2221 tgaagttgtg ccgggcccga cgctgcacga ccaggacgcc ccgtaccccc gagcccacga
     2281 acaccggggc g
//
LOCUS       DASWGG010000002         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1612829, whole genome shotgun sequence.
ACCESSION   DASWGG010000002 DASWGG010000000
VERSION     DASWGG010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612829"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /gene="rph"
                     /locus_tag="VF954_00030"
     CDS             <1..554
                     /gene="rph"
                     /locus_tag="VF954_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX9529490.1"
                     /translation="AEYSLLPGSTSERTDREAAKGKQSGRTQEIQRLIGRSLRAVTDM
                     RAMGEVQIVLDCDVLQADGGTRTAAICGAYVALHDAFSRLMQKGKLAAHPLTESCGAI
                     SVGVVDALCLLDLDYSEDVRAEVDMNLVMTSSGRFIEVQGTAEGVPFSRTELDELLSL
                     GEHGIAQLLELQASVLAEPPPPR"
     gene            551..1135
                     /gene="rdgB"
                     /locus_tag="VF954_00035"
     CDS             551..1135
                     /gene="rdgB"
                     /locus_tag="VF954_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX9529491.1"
                     /translation="MTRELVLATANADKAAEIAAILAGWSLVPRPAGIPDVEETEDTL
                     EGNARLKARTLVSATGLAAVADDTGLSVVALGGAPGVRTSRYAGEGATYAENVAKLLV
                     SLEGVDYRRAHFRTVALVAFPDGSSIAASGVVAGTIASSARGEGGFGYDPVFVPDEGG
                     GLTFAEMAPGHKHAISHRGRAFRELAARLPDHLR"
     gene            1785..>2303
                     /locus_tag="VF954_00040"
     CDS             1785..>2303
                     /locus_tag="VF954_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529492.1"
                     /translation="MGDANTGADSRVRAGVALLAAAAGAWAVAASRAIFPFGSINHDE
                     AVYRLQADTLTHGHLFPVAPPHPAAFLPWLSAYRAGHFIPKYAPVHATVLAVGRLLAG
                     TDRAGLAIIAASAVAATYLVGVEVLGDKVQALAAAGLLLASPLFLLQSATYLTYTTGL
                     VLLELFAVAVLRA"
BASE COUNT          296 a          807 c          827 g          373 t
ORIGIN      
        1 cggcggagta ctcgctgctg cccggctcca cgtcggagcg gaccgaccgg gaggcggcca
       61 agggcaagca gtcggggcgc acccaggaga tccagcgcct catcggccga tcgctgcggg
      121 ccgtgaccga catgcgggcc atgggcgagg tgcagatcgt gctcgactgc gacgtgttgc
      181 aagccgacgg cgggacgcga acggcggcca tctgcggcgc ctacgtcgcg ctgcacgatg
      241 ccttcagccg cttgatgcag aaggggaaat tggcggccca tccgctcacc gagtcctgtg
      301 gcgccatctc ggtcggggtg gtcgacgcct tgtgcctgct cgacctcgac tactccgagg
      361 acgtgcgcgc cgaggtcgac atgaacctgg tcatgacctc ctccggccgt ttcatcgagg
      421 tacagggcac cgccgagggc gtgcccttca gccggacgga gctcgacgag ctgctcagcc
      481 tcggcgagca cggcatcgcc cagctcctcg agctccaggc ttcggtgctg gcggagccgc
      541 ccccgccccg gtgacccgcg agctggtcct ggccaccgcc aacgccgaca aggcggccga
      601 gatcgcggcc atcctggcgg ggtggtcgct tgtgccccgc ccggccggca taccggacgt
      661 cgaggagacg gaagacactc tcgaaggcaa cgcccgcctg aaggcccgga cgctggtctc
      721 ggccaccggg ctggcggcgg tggccgacga caccggcctg tcggtcgtgg cgctcggtgg
      781 ggcgccgggc gtgcgcacgt cccgctacgc cggcgagggg gccacctatg ccgagaacgt
      841 ggccaagctg ctcgtctcgt tggagggcgt ggactatcgc cgagcccatt tccggaccgt
      901 cgcgctggtc gccttcccgg acgggtcctc gatcgcggcg tcaggggtcg tggccgggac
      961 catcgcgtcg tcggctcggg gcgagggggg gtttgggtac gatcccgtgt tcgtgcccga
     1021 cgagggcggg gggctcacgt tcgcggagat ggcccctggt cacaagcacg ccatcagcca
     1081 ccgggggcgc gccttccggg agctggcggc ccgcctgccg gatcaccttc gctagtgcca
     1141 gccgggctcc ggcgactagc tgctcgggtc ggggcttgag tgggtgccgc cgccgcttcc
     1201 gtgtccggtc ggtggggccg ggcgcggccc accggttgtc gacgtccagc ctgtcgaccc
     1261 ggaagattcg ccactggccg tcgtcggtgt ggggctggat gactccaccg tgctcgtcgt
     1321 cgtcgtgaac ctaacctcgg cgtgggtctc ggtcccgcga gtggccgggg cggtggtggc
     1381 gcgcgccgta cctgccttgg cggtctctgg ggcctcagtg gtctcagtgg cctcggtgcc
     1441 ttccggcgag gaggcttcag tcgccgcccc gggagtcgtc gacttcttct ccagcccggg
     1501 agggcccgag tgacagtggt tcttgtcacc gaagccccat ccgggcttgc acggggtgta
     1561 gccgtagcca tagccgccac cgcccctggg tcggttggcg gccgcgaggg cggcgctgcc
     1621 catcagccct gcgaacacga agatcaacac gcccacgcgc acggtacgac tcaacggtct
     1681 tctcctgacg aggtgacaag aaccactcgg ggttggccgt cgccggcgcg gcacgaactc
     1741 accgccaggt ctgaagcgac gccgtggcct acccttcccg gcccatggga gatgcaaaca
     1801 caggggccga ctcacgggtg cgagcaggag ttgcgctcct cgcggccgcc gccggggcgt
     1861 gggcggtcgc cgcgtcgcga gccatatttc cgtttggctc catcaaccac gacgaagccg
     1921 tctaccggct tcaggcggac acgctcacgc acgggcacct gttcccggtg gcgcctcctc
     1981 acccggccgc cttcctgccg tggctctcgg cgtaccgcgc cggccatttc atcccgaagt
     2041 acgcgccggt tcacgccacg gtgctcgccg tgggacgttt gctcgccggg acggaccgcg
     2101 ccgggctggc gatcatcgcc gcgtccgcgg tcgcggcgac ctacctggtc ggggtggaag
     2161 tgctgggcga caaggtccaa gccttggcgg ccgcgggcct cctgttggcc tcaccgctct
     2221 tcctgctgca gtcggcgacg tacctcacct acacgacggg actggtgttg ctcgagctgt
     2281 tcgccgtcgc ggtgttgcga gcg
//
LOCUS       DASWGG010000003         4180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_653152, whole genome shotgun sequence.
ACCESSION   DASWGG010000003 DASWGG010000000
VERSION     DASWGG010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653152"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1193)
                     /gene="rsmH"
                     /locus_tag="VF954_00045"
     CDS             complement(168..1193)
                     /gene="rsmH"
                     /locus_tag="VF954_00045"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX9529493.1"
                     /translation="MEDPYSARFHLCSPGRHPGSNLPVEGLRMSQAFAHEPVMTAEIV
                     AIFAPVPPGTVLDATVGGGGHAAALLAAHPQLRVVGLDRDPDAAAAAAQALAPWAGRV
                     SLHHARFDRLSEVAARLGLARLSGALFDLGVSSPQLDRPERGFSYRNDAPLDMRMDPT
                     QTRTATDVVNGWAERDLASLLRANGEGRFGGRIARAIVAHRPVTTTVQLAAVVRDAIP
                     AATRRTGGHPARRAFQAIRIAVNDELDVLPGALDQAIDLLAPGGRIAVLAYHSGEDRI
                     VKDRFRTAETGGCECPPGLPCACGAEPRVVPVFRGVRRPGPEELGRNRRAESARLRAA
                     ERLETAR"
     gene            complement(1299..1757)
                     /locus_tag="VF954_00050"
     CDS             complement(1299..1757)
                     /locus_tag="VF954_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX9529494.1"
                     /translation="MARFFGRYEHSLDLKGRVILPARFRPPFEHGGFLTQFHDGCLAL
                     WTPDEFDKQMAEMLASSEAGPSERNLARVWASGTHEVEVDRQGRMPIPSFLRGFAALE
                     GDVLVHGAIDRVELWSPQTWEQRVKPIERTLTEGTRPTKGTVRTEGPEDH"
     gene            1916..2347
                     /locus_tag="VF954_00055"
     CDS             1916..2347
                     /locus_tag="VF954_00055"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX9529495.1"
                     /translation="MSTGPRSVVEALVASLNAHDGRASRALFAPDAVVVTASGRRTDL
                     DGMDRIHASTLDAFPDLRLRVTRWVVGGDTVVTEEVMDGTHRGEIGGFEPTGRSVALR
                     MVHVTRVVDGLVVERVSYHDAAGFLAQLAGTTDQPAGASAP"
     gene            complement(2355..3020)
                     /locus_tag="VF954_00060"
     CDS             complement(2355..3020)
                     /locus_tag="VF954_00060"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529496.1"
                     /translation="MSWSFESRHEPAAELHRRDPWARPGRSVTWCQPSGPAVVLGSTQ
                     PLEAADAAACRRRGAEVVRRRTGGGAVLVEPAAIVWVDLVIGRDDPLWSADVGRAFLW
                     VGAAFARALGRVGVRGVVHPGPLRRGRWSAAVCFAGLGYGEVTVGGAKVLGLAQRRSR
                     DAALFQCAVPLRWRPADLVALLAEPRPAAADLEGAALGLGEVAPAVTEASVVEALLDA
                     LPA"
     gene            complement(3017..3529)
                     /locus_tag="VF954_00065"
     CDS             complement(3017..3529)
                     /locus_tag="VF954_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX9529497.1"
                     /translation="MARALVLNATYEALCVVSTRRALLLILDGKAELVSEGEGLFRSV
                     RLCLPEPSIVRLEHYVKVPYQARLALNRRAVFARDGHRCQYCGSPAENIDHVVPRSRG
                     GSHSWDNVVAACRPCNARKEDRMLHESGMILRHVPTQPRERTWILAASGALRPDWEPF
                     LAAMPAARLA"
     gene            complement(3627..3893)
                     /locus_tag="VF954_00070"
     CDS             complement(3627..3893)
                     /locus_tag="VF954_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529498.1"
                     /translation="MKGQPGEPWRRAALGVVPHPTLWAEAVVEVFVLARPGWWRRWPP
                     LPTPAPGYLRFRMVTQYGQPDRRPEARDVVSYLRWCRHMRLVRQ"
     gene            complement(3890..>4180)
                     /locus_tag="VF954_00075"
     CDS             complement(3890..>4180)
                     /locus_tag="VF954_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529499.1"
                     /translation="RPAAEAGSEVRARAGRLVGRAVASVANLLDLPLAVVGGSVALGY
                     GPVFFAGAQAEIDARCGLDFSRGTRIAPAGLGEAGPLVGAGAVGWRGLRGPG"
BASE COUNT          553 a         1610 c         1539 g          478 t
ORIGIN      
        1 cccgaacagc ccgcagcaca cgaaggcccc cgtggcggca gccagccagc gggtccggcg
       61 ccggcgggcc cggacgctcg gttcgccggg ctcgacgacc cgcagcgggg cccgttgcgg
      121 acggggccgg agcccgtcgg gccggacggc cggccgggtg gtcgcggtca ccgagccgtc
      181 tccagccgct cggcggcgcg caaccgggcg ctctcggccc gccggttgcg cccgagctcc
      241 tcgggcccgg gtcgccgaac gccccggaac accggcacca ccctgggctc ggccccgcac
      301 gcgcacggca ggcccggcgg gcattcgcag cctccggtct ccgccgtccg aaagcggtcc
      361 ttcacgatgc ggtcctcgcc ggagtggtac gccagcacgg cgatccgccc tccgggcgcc
      421 agcaggtcga tcgcctggtc cagggcgccg ggcagcacgt cgagctcgtc gttcaccgcg
      481 atcctgatgg cctggaacgc ccgccgggcg ggatggccgc cggtgcgtcg cgtcgccgcc
      541 gggatggcat cgcgcacaac ggcggcaagc tgcacggtgg tcgtgacggg ccggtgggcg
      601 acgatggctc gggcgatgcg gccgccgaag cgcccctcgc cgttggcgcg caacagggag
      661 gcgaggtcgc gctcggccca tccgttcacc acgtcggtcg ccgttcgggt ctgggtcggg
      721 tccatgcgca tgtccagggg tgcgtcgtta cggtaggaaa agccccgttc ggggcgatcg
      781 agctgtggcg agctgacgcc gagatcgaac agcgccccgc tcagccgggc gaggcccagg
      841 cgggcggcca cctccgacag gcggtcgaag cgagcgtggt gcaacgacac ccgcccggcc
      901 cagggggcca gggcctgagc ggcggcggcg gcggcgtcgg ggtcacggtc gaggcccacg
      961 acgcgcagct gcgggtgggc cgccagcagg gccgcggcgt gcccgccgcc gccgacggtg
     1021 gcatccagca ccgtcccggg cgggaccggc gcgaagatgg ccacgatctc ggcggtcatc
     1081 accggctcgt gggcgaacgc ctggctcatc cgcagcccct cgacaggcag gttgctgccg
     1141 ggatgtctcc ccggcgagca gagatggaag cgggcggagt agggatcttc caccgcgccg
     1201 gtcgaggggc tgcgcaggag ccggggcggc cccggcgggg gtttggggtg tggagtggcg
     1261 tcgtcgtggt ggcttggcct ccttgtcctg ggtcccgcct agtggtcctc cgggccttcc
     1321 gtgcgcacag tgccttttgt gggccgggtg ccttcggtca gcgtgcgctc gatgggcttg
     1381 acccgctgct cccatgtctg cgggctccac agctccaccc ggtcgatggc cccgtgcacc
     1441 agcacgtcgc cctcgagggc ggcgaagccc cggagaaagc tcgggatcgg catccgcccc
     1501 tggcggtcga cctccacctc gtgggtcccc gaggcccaga cccgggccag gttgcgctcc
     1561 gacggcccgg cctccgagct ggccagcatc tcggccatct gcttgtcgaa ctcgtcggga
     1621 gtccagaggg ccaggcagcc gtcgtggaac tgcgtgagga agccgccgtg ctcgaagggc
     1681 ggacggaagc gcgccgggag gatcacccgc cctttcaggt cgagggagtg ctcgtacctc
     1741 ccgaagaaac gcgccacgac tcactcccga tcgctcctcg tgacggcccg gtcctcccct
     1801 tcgcgccaca agtcgccacc ttacgcccca ctcttccacc cgtcaacaac tcgcccaccc
     1861 ccgggccccc caatcggcgc gacgatgagg ggatgagcac cgcgacggag tcgccatgag
     1921 cacgggcccg cgttcggtcg tggaggccct cgtggcctcc ctgaacgccc acgacggccg
     1981 ggccagcagg gcgctgttcg ctcccgacgc cgtcgtcgtc accgcctcgg gtcgccggac
     2041 cgacctggac gggatggacc gcatccacgc ctcgaccctc gacgcctttc ccgacctgcg
     2101 cctgcgggtg acccggtggg tcgtcggcgg cgacacggtg gtgaccgagg aggtgatgga
     2161 cggcacccac cggggcgaga tcggtgggtt cgagccgacc ggccggtccg tcgccctgcg
     2221 catggtgcac gtcacccgcg tcgtggacgg gctggtcgtg gagcgggtct cgtaccacga
     2281 cgcggccggc ttcctggccc agctggccgg cacgaccgat cagccggcgg gcgcctcggc
     2341 cccctgagag ccgctcaggc cggcagggcg tccagcaggg cctcgacgac gctggcctcg
     2401 gtgacggccg gggccacctc gccgaggccg agggccgcgc cctcgaggtc cgcggccgcc
     2461 ggccgcggct cggcgaggag ggcgaccagg tcggccgggc gccagcgcag ggggacggcg
     2521 cactggaaga gggcggcgtc cctggagcgg cgctgggcca ggccgagcac cttggccccc
     2581 ccgacagtta cctcgccgta gcccagcccg gcgaagcaca ccgccgccga ccaccgaccg
     2641 cgccgcagcg ggccagggtg cacgacgccc cgaacgccga cgcggccgag ggcccgggcg
     2701 aaggcggcac cgacccagag aaaggcgcgg cccacatccg cgctccacaa cgggtcgtcc
     2761 cggccgatga ccaggtcgac ccacacgatg gcggccggct cgaccagcac ggctcccccg
     2821 ccggtcctgc gccgcacgac ctcggcaccg cgccggcggc aggcggcggc atcggctgcc
     2881 tcgaggggct gggtgctccc cagcacgacc gccggccccg acggctggca ccacgtcacc
     2941 gatcggccgg gccgggccca cgggtcccgg cggtgcagct cggccgcggg ctcgtggcgt
     3001 gactcgaagg accagctcac gccagccggg ccgccggcat cgccgcgagg aacggctccc
     3061 agtcgggccg cagggctccc gaggccgcca ggatccaggt gcgctcgcgg ggctgggtgg
     3121 gcacgtggcg caggatcatg ccggactcgt gcagcatccg gtcctccttg cgggcgttgc
     3181 agggccggca cgcggccacc acgttgtccc agctgtggga gccgcccctg ctccggggca
     3241 cgacgtggtc gatgttctcc gcgggcgagc cgcagtactg gcagcggtgc ccgtcccggg
     3301 cgaagacggc ccgccggttg agcgccaacc gggcctggta cgggaccttg acgtagtgct
     3361 cgaggcgaac gatcgacggc tccggcaggc acagccgcac ggacctgaac aggccctccc
     3421 cctccgacac cagctcggcc ttgccgtcca ggatcaggag caacgcccga cgggtcgaca
     3481 ccacgcacaa cgcctcgtag gtcgcattca gcacgagcgc ccgcgccacc gctcagccct
     3541 ccgaccgccc tgccggcgag cgccgccgac gcggccggcc tcgagccatg ggtggaagcg
     3601 ccatgggcag gaactctact cggggctcac tgccggacca ggcgcatatg ccggcaccaa
     3661 cgcaggtagc tcaccacgtc ccgagcctcg ggccgccggt ccggctggcc gtactgggtg
     3721 accatccgga accgaaggta cccgggcgcc ggggtgggca ggggcggcca gcggcgccac
     3781 cagcccgggc gggccaggac gaacacctcg accaccgcct cggcccacag cgtggggtgc
     3841 gggaccacgc ccagggcggc gcggcgccac ggctcgccgg gctgcccttt cacccgggtc
     3901 cccgaaggcc gcgccagcca accgcgccgg ccccgaccag ggggccggcc tcgcccagcc
     3961 ccgccggggc gatccgcgtc ccccgcgaga agtcgagccc gcagcgggcg tcgatctcgg
     4021 cctgggcccc ggcgaagaac accggcccgt agcccagggc cacgctgcct cccaccaccg
     4081 cgaggggcag gtccagcagg ttggctaccg acgccacggc gcggcccacg agccggcccg
     4141 cccgggcccg cacctcggag ccggcctcgg ccgccggccg
//
LOCUS       DASWGG010000004         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1297574, whole genome shotgun sequence.
ACCESSION   DASWGG010000004 DASWGG010000000
VERSION     DASWGG010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297574"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1469)
                     /locus_tag="VF954_00080"
     CDS             complement(162..1469)
                     /locus_tag="VF954_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529500.1"
                     /translation="MAAAGLAVLTTIASGPGAWAQTPGPDLPSVAMAPTESHTNDPNK
                     GQWFVFQLQAGQSGRTKASIANPADVAQTVSLSVRDLTFSPEGAPQINDGPTQTDVGA
                     WSTVGTPTVTVPAKGHVLVPFSVAVPTNADPGDHSGVVVATSRPEPTAGGQTLVKRVA
                     VRLYVTVPGDATRAFEVASVRSSPDSALFPRRETVRVRLRNTGRIRLHPTVTVAGRAA
                     VGADTMIAQTYEDYYAQVPVGFAGGPVNEKVVATTDAGPARVVPHSQFVIPWGVIAAL
                     LVGGFILLGLAMLVRRRQRKLAALHADLRRLEQLVTQRPGMTQGRGVLDPPPDAPGPA
                     DTSGEVRAIETAIKRARRTGHPETLPELAVALRAAGGDALDVLLEALPDCGADRRDEL
                     VAAIRAHPEARVGASHRLRALPPDLAARLTAPRRAIRKSASKT"
     gene            complement(1534..2097)
                     /locus_tag="VF954_00085"
     CDS             complement(1534..2097)
                     /locus_tag="VF954_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529501.1"
                     /translation="MHKRLGTIALGLGLAGALVALPAAGYASTSMSTATLNANILAGS
                     VGARTIQSVTPGAMTSALGSPTLSTTLAVVVDEAAVSGDANGWTVTVQSSPFTDSGTD
                     TIVASALSDSANTAIPVCVGCTETVTGPATPGTLDAARTLISTTGELNSVLYTGTYTS
                     NSSLVLTPPNGTVAANYHATLTVTLTQ"
     gene            complement(2283..>3115)
                     /locus_tag="VF954_00090"
     CDS             complement(2283..>3115)
                     /locus_tag="VF954_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9529502.1"
                     /translation="QPAGAWNARAGHVAATVAPPVVVAWALALILAVLAEVRHDRQAH
                     QVERATSAFLAVAGHELRTPLTSIRGFSQTLAQRWDSLPAGRRQEMVQAIARQSRLLE
                     HLIERLLTAARLEAGTSSTLLLGPVDLTAVLQDVVEAHQPLAPLHEIRVEMAPGLTVH
                     GDGGALGQVLGHLVENAVKYSPGGGLVTVSATRLRRRVEVAVEDDGVGLPSDFPRLFD
                     RFAQAEPVDTRTYDEGGMGLGLFIARTLVEQMGGVIRAEPRQPRGARFVVRLVAAGDD
                     "
BASE COUNT          405 a         1040 c         1241 g          429 t
ORIGIN      
        1 agcatctcga tcaccagcgc cgggcagacc aagatccagg tcagctgagg tccggcggcg
       61 cgcgccccgc agcgcgaggg caacggcggt gggagcctcg gccgaccggt tccgctcccg
      121 tgtaggaccg gccggccggg ctccgcgctg agcctcccgg ctcaggtctt ggaggcgctc
      181 ttcctgatgg cgcggcgcgg ggcggtgagc cgggcggcca gatcgggggg cagggcgcgc
      241 aggcggtgac tggccccgac ccgtgcctcg gggtgggcgc ggatggcggc cacgagctcg
      301 tctctgcggt cggcgccgca gtcgggcagg gcttccagca gtacgtcgag ggcgtcgccg
      361 cccgcggcgc ggagggccac ggctagctcg ggcagggtct ccggatggcc ggtgcggcgg
      421 gcccgcttga tcgccgtctc gatggcgcgg acctcgcccg acgtgtcggc cgggcccggg
      481 gcatcaggcg gtgggtccag gacgccccgg ccctgggtca tgccggggcg ctgggtgacg
      541 agctgctcga gacggcgcag gtcggcgtgc agggcggcca gcttgcgctg gcgccgtcgt
      601 acgagcatgg ccagccccaa caggatgaac ccgcccacca ggagggcggc gatgacgccc
      661 cacgggatga cgaactgcga gtggggcacc acccgggccg ggccggcgtc ggtggtggcg
      721 acgaccttct cgttgaccgg gccgccggcg aagccgaccg ggacctgggc gtagtagtcc
      781 tcgtaggtct gggcgatcat ggtgtcggcg ccaactgccg cccggcctgc gacggtcacg
      841 gtggggtgga gccggatgcg gccggtgttg cgcaacctga cccgcaccgt ctcccggcgg
      901 gggaagaggg ccgagtccgg cgaggaccgc accgacgcga cctcgaacgc ccgggtggcg
      961 tctccgggca cggtgacgta gaggcggacg gcgacccgct tgacgagcgt ctggccaccg
     1021 gcggtcggct ccggccggga ggtggccacc accaccccgg agtggtcgcc cgggtcggcg
     1081 ttggtcggca cggcgacgga gaaggggacg agcacatggc ccttggccgg gacggtgacg
     1141 gtcggggtgc cgaccgtcga ccaggcgccc acgtcggtct gggtggggcc gtcgttgatc
     1201 tggggggcgc cctccggtga gaacgtgagg tcccggaccg acagcgacac ggtctgggcc
     1261 acgtcggccg ggttggcgat gctggccttc gtccggcccg actggccggc ctggagctgg
     1321 aacacgaacc attggccctt gttgggatcg ttggtgtggc tctcggtcgg agccatggcg
     1381 accgaaggca ggtccgggcc aggggtctgg gcccacgcgc cgggcccgct ggcgatggtc
     1441 gtgagcacgg cgaggcccgc cgccgccatg agggtgacgg cgggccggcc gcgaggctgg
     1501 ggagcccggg agcgcaaggt gtcggctgtg cctttactgg gtgagggtga cggtgagggt
     1561 ggcgtggtag ttggcggcca ccgtcccgtt gggaggggtc agcacgagcg agctgttgga
     1621 ggtgtaggtg cccgtgtaca gcacgctgtt gagctcgccg gtggtggaga tgagcgtccg
     1681 ggccgcgtcg agggtgcccg gcgtggccgg tccggtcacg gtctcggtgc agcccacgca
     1741 caccggtatc gcggtgttgg ccgagtcgct gagcgccgat gccacgatcg tgtcggtgcc
     1801 gctgtcggtg aacggcgacg actgcacggt caccgtccag ccgttggcgt ccccgctgac
     1861 cgccgcttcg tcgaccacga ccgcgagcgt ggtggacagg gtgggggagc ccagggcgga
     1921 ggtcatggcg cccggcgtga cgctctggat ggtgcgggcg ccgacgctgc cagccaggat
     1981 gttggcgttg agcgtggcgg tgctcataga ggtggacgca tagccggcag ccggcagggc
     2041 gacgagggcg ccggcgagcc ccagtcccag tgcgatcgtt cccagtcgct tgtgcatcgt
     2101 cgatagctac ccttctcgtc ggtggtcggt cggagctgtg gggaagcgga acccacatca
     2161 gttatcgcct cggatgacgt tccactcaat ggccggggcg tgtcatttca cgatgggcaa
     2221 accgggccat ggcggggcgg ccgacgtgac taaggtcggc cgccgggtct gggtcgaggt
     2281 gcctagtcgt cgcccgcagc caccagccgg acgacgaagc gagcgccccg aggttggcgg
     2341 ggctcggccc ggatgacgcc gcccatctgc tccaccagcg tgcgggcgat gaacagcccc
     2401 aggcccatgc caccctcgtc gtaggtccgg gtatcgaccg gttcggcctg ggcaaagcgg
     2461 tcgaagagcc gcgggaagtc cgatggcagg cccaccccgt cgtcctcgac ggcgacctcg
     2521 acccgccgcc ggaggcgggt ggccgacacg gtgaccagcc ctcctccggg cgagtacttc
     2581 accgcgttct ccaccaggtg gcccagaacc tggccgagcg ccccgccgtc gccgtgcacc
     2641 gtcaacccgg gcgccatctc gacccggatc tcgtgcaggg gtgcgagggg ctggtgcgcc
     2701 tcgacgacgt cctgcagcac ggccgtcagg tccacgggcc ccagcagcag ggtcgacgat
     2761 gtccccgcct ccaggcgggc ggccgtcagc agccgctcga tcaggtgctc gagcaggcgg
     2821 gactggcggg cgatggcctg gaccatctcc tggcgccggc ccgccggcag ggagtcccag
     2881 cgctgggcca gcgtctggct gaagccccgg atcgacgtga gcggcgtgcg cagctcgtgg
     2941 ccggccacgg ccaggaaggc ggaggtggcc cgctccacct ggtgggcctg gcggtcgtgg
     3001 cggacctctg cgagcacggc gaggatcagt gccagcgccc aggccaccac gacgggaggt
     3061 gccaccgtcg cggcgacatg gcccgcccgc gcgttccacg ccccggccgg ctggg
//
LOCUS       DASWGG010000005         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1938740, whole genome shotgun sequence.
ACCESSION   DASWGG010000005 DASWGG010000000
VERSION     DASWGG010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938740"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..900)
                     /gene="rfbD"
                     /locus_tag="VF954_00095"
     CDS             complement(61..900)
                     /gene="rfbD"
                     /locus_tag="VF954_00095"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX9529503.1"
                     /translation="MRILITGAGGQLGHDLARACATDDLAACDRAALDVADRDRVLQV
                     ILSVRPHAVIHAAAWTAVDACEGDPDRAFQVNALGTRHVAEAARRAGAHLVYVSTDYV
                     FDGRSPRPYREWDAPNPLSVYGQSKLAGEREAGPDATVVRTSWLCGAHGQNVVRTVLR
                     LAAERDRLAFVDDQHGCPTFTEDLAGALRDLAVARRPGVFHVTNQGPTTWFELARAVL
                     AAAGLDPGRVEPIPTSALQPPRPAPRPASSVLDNAALRLSGLPLLPYWHEPLGRLVKE
                     LTA"
     gene            complement(897..>1845)
                     /gene="rfbB"
                     /locus_tag="VF954_00100"
     CDS             complement(897..>1845)
                     /gene="rfbB"
                     /locus_tag="VF954_00100"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX9529504.1"
                     /translation="ITGGAGFIGSNFVRHVLASSDDEVTVYDALTYAGNLSTLADVEE
                     SPRYHFVKGDVCDRDAAEEAMRGCDAVVHFAAESHVDRSILSPDEFVHTNCQGTNVVC
                     DVARRLEVGRVVHVSTDEVYGSVEEGSSGESDPVAPRSPYSASKAGSDLIALSYVTTY
                     GLPLVVTRSSNNFGPYQHPEKVIPLFVTNLLAGRRVPLYGDGLNVRDWCYVGDNCAGV
                     DLVLRHGAVGEIYNVGAGNGVSNLELTRRILALMGMGEEMVEPVADRLGHDRRYSIDT
                     AKVRALGWSPRASLDDALASTVDWYRANEWWWGPLKAAA"
BASE COUNT          267 a          652 c          670 g          256 t
ORIGIN      
        1 gggccaggta ggcgcccgtg gtgagcccga cgtagcccgt gcccacgacc gcgacagagc
       61 tcacgccgtt agctccttca ccaggcgacc gaggggctcg tgccagtagg gcaggagcgg
      121 gagaccggag aggcgaaggg cggcgttgtc cagcaccgag ctcgccggcc ggggcgcggg
      181 ccggggcggc tggagcgcgg aggtcgggat cggctcgacc cgcccggggt cgaggccggc
      241 agcggccagg accgcccggg ccagctcgaa ccacgtggtg ggcccctggt tggtcacgtg
      301 gaacaccccc ggccgccggg cgacggcgag gtcgcgaagc gcgccagcga ggtcctccgt
      361 gaaggtcggg cagccgtgct ggtcgtccac gaacgccagc cgatcccgct ctgctgccag
      421 gcgcagcacg gtcctgacca cgttttgacc gtgggcgccg cacaaccagg aggtcctcac
      481 gacggttgcg tccgggccgg cctccctctc gcccgcgagc ttggattggc cgtagaccga
      541 cagggggttg ggcgcgtccc attcccggta gggccgcggc gaacgaccgt cgaagacgta
      601 gtcggtcgag acgtagacga ggtgggcccc ggcccggcgc gccgcctcgg cgacgtggcg
      661 cgtgccgagc gcgttgacct ggaaggcccg gtccgggtcc ccttcgcacg cgtcgaccgc
      721 cgtccaggcc gcggcgtgga tcacggcgtg aggtcggacc gacaggatca cctgcaggac
      781 gcggtcgcga tcggccacgt cgagggcagc gcggtcacac gccgccaggt cgtcggtggc
      841 gcaggccctg gccaggtcgt ggcccagctg gccccccgcc ccggtgatca ggatcctcat
      901 gccgcggcct tgaggggccc ccaccaccac tcgttggcgc gataccagtc gaccgtcgat
      961 gccagcgcgt cgtccaggct cgcccgtggt gaccagccca gagcacggac cttggcggtg
     1021 tcgatcgagt accggcggtc gtggcccagg cgatcggcca ccggctcgac catctcctcg
     1081 cccatcccca tgagggccag gatccgccgc gtcagctcga ggttggacac cccgttgccg
     1141 gccccgacgt tgtagatctc cccgaccgct ccatggcgca gcaccaggtc gacgccggcg
     1201 cagttgtcgc ccacgtagca ccagtcgcgg acgttcaggc cgtccccgta caacggcacc
     1261 cggcggccgg ccaacaggtt ggtcacgaag agcgggatga ccttctccgg gtgctggtag
     1321 ggcccgaagt tgttggacga cctggtcacc acgagaggca gcccgtaggt cgtcacatag
     1381 ctgagggcga tcaggtccga gcccgccttg gaggcgctgt agggcgaccg gggcgcgacc
     1441 gggtccgact cccccgacga gccttcctcg accgagccgt agacctcgtc ggtcgagacg
     1501 tgcaccacgc ggccgacctc gaggcgccgg gccacgtcgc agaccacgtt ggtgccctgg
     1561 cagttggtgt ggacgaactc gtcggggctg aggatggacc ggtccacgtg gctctcggcc
     1621 gcgaagtgca cgaccgcgtc gcagcctcgc atggcctcct cggcggcgtc gcggtcgcag
     1681 acgtcgccct tcacgaagtg gtatctgggg ctctcctcga cgtccgccag cgtggacagg
     1741 ttgccggcgt aggtgagggc gtcgtacacc gtcacctcgt catcgctcga ggcgagcacg
     1801 tggcggacga agttggaccc tatgaacccg gccccgccgg tgatg
//
LOCUS       DASWGG010000006         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2910678, whole genome shotgun sequence.
ACCESSION   DASWGG010000006 DASWGG010000000
VERSION     DASWGG010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2910678"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VF954_00105"
     CDS             complement(<1..994)
                     /locus_tag="VF954_00105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9529505.1"
                     /translation="MRVDGGIGADLTKVPQAAQETEAAGYDGAWSAETGHDPFFPLLL
                     AAGSTEHLELGTGIAVAFARNPMTLAVTANDLQLHSRGRFLLGLGSQIKAHVEKRFSM
                     PWSHPAPRMRELVLAMRSIWDCWNNGTKLDFRGEFYRHTLMTPFFNPGPNPYGPPKVF
                     LAGVGELMTEVAGEVCDGFLVHGFTTERYLREVTLPALDRGLAKAGRKREDFQVSYPA
                     FVVTGTDEEELAKAAQATRQQIAFYGSTPAYRPVLDLHGWGDLQTELNSLSKQGEWVK
                     MGELIDDDVLNAFAVVSDPESVPKRIVARVGDVVDRVSFYAPYASDPQRWRNMLA"
     gene            complement(1038..1898)
                     /locus_tag="VF954_00110"
     CDS             complement(1038..1898)
                     /locus_tag="VF954_00110"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX9529506.1"
                     /translation="MTLLVRHLDTHPSEPVMVVALDGWIDAGLGAKAAVANVLEQIST
                     ELMATFDPDELIDHRARRPTLRLIDGVIDRVTWPEIELRVGEDANSKAALILVGPEPD
                     MRWHAFTDAVVDLARSLDVRLVVGLGAFPAPVPHTRPTRVAATATSPELAGTVGYVSG
                     TIEVPAGVHAPIEAALGQAGIPAVGIWARVPHYLSGMPYPAAAAALLESLAAVSGLAL
                     DSSSLQAAATSTRTRVDALIAQSEEHTAMVRQLEEHVDAAESAAFGGGDIPSGDELAA
                     ELERFLRGEL"
     gene            complement(1903..2931)
                     /locus_tag="VF954_00115"
     CDS             complement(1903..2931)
                     /locus_tag="VF954_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX9529507.1"
                     /translation="MEAIDEARVTRWFEANIEGAAPPLSFELVAGGRSNLTFRVADAA
                     GHDYVLRRPPVSHLLPTAHDMVREHRIISCLGPTAVPVPPALGLCTDESVNQRPFYVM
                     GYVEGHVLRDAAMVEKVLDVEQRRRAGQDLADVLAGIHAVDVDAVGLGDLGRREGYIA
                     RQLKRWYAQFQSSNPLVGRPVPAIDEVHDVLAARIPDQGPATIVHGDYRLDNTMVGDD
                     GTVQAVLDWEICALGDPLADVGLLMVYWTEDGDRDPALGVAPTALPGFPTRAELRARY
                     AERSGRDLSALDFYVAFGYWKLACILEGVMSRYLGGAGGGDRSGYEGFGQQVERLAES
                     ARAAAAGL"
     gene            2964..>3490
                     /locus_tag="VF954_00120"
     CDS             2964..>3490
                     /locus_tag="VF954_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9529508.1"
                     /translation="MTDVTDASFEQDVLARSDEVPVVVDLWAPWCGPCKTLGPILERV
                     VGATDGQVDLVKVNVDDNPNISAAFRVQSIPAVYAIAKRQVVDSFVGALPEPAVREWV
                     SRLAPPPSEADRLTAKGDEASLRQALEIDPGHQAAVEKLAELLVDQGQAEEALGLLAR
                     VPETADTRRIGALARL"
BASE COUNT          489 a         1205 c         1316 g          480 t
ORIGIN      
        1 cggcgagcat gttgcgccac cgctgagggt cggaggcgta cggggcgtag aagctcaccc
       61 ggtcgacgac gtcgcccacc cgggccacga tgcgcttggg caccgactcc gggtcggaca
      121 ccacggcgaa ggcgttgagc acgtcgtcgt cgatcagctc gcccatcttc acccactcgc
      181 cctgcttgga cagcgagttg agctcggtct gcaggtcgcc ccagccgtgc aggtcgagca
      241 cggggcggta ggcgggcgtc gagccgtaga aggcgatctg ctggcgcgtg gcctgggccg
      301 ccttggccag ctcctcttcg tcggtgccgg tgacgacgaa ggccgggtag gagacctgga
      361 agtcctcgcg cttgcgcccc gccttggcca ggccccggtc cagcgccggg agggtcacct
      421 cgcgcagata gcgctcggtg gtgaacccgt ggaccaggaa cccgtcgcac acctcgccgg
      481 ccacctcggt catgagctcg cccacgccgg ccaggaacac cttcggcggg ccgtaggggt
      541 tggggccggg gttgaagaac ggcgtcatca gggtgtgccg gtagaactcg ccccggaagt
      601 cgagcttggt gccgttgttc cagcagtccc agatggagcg catggcgagg accagctcgc
      661 gcatgcgtgg agccgggtgg gaccacggca tggagaaccg cttctcgacg tgggccttga
      721 tctggctgcc caggccgagc aggaaccggc ccctggagtg cagctggagg tcgttggccg
      781 tgaccgcgag cgtcatgggg ttgcgggcga aggcgaccgc gatccccgtc cccagctcga
      841 gatgctcggt gctgcccgcc gccagcagca gcgggaagaa cgggtcgtgg ccggtctccg
      901 cgctccaggc gccgtcgtac ccggccgcct ctgtctcctg ggccgcctgc ggcaccttgg
      961 tcagatcggc tccgattccc ccatcaacgc gcatgggcgt ggaccctagc gccgcgacga
     1021 cctcgccggc cacgagctca cagctcgccg cgcaggaagc gctccagctc ggcggccagc
     1081 tcgtcgccgg aggggatgtc gccgcccccg aaggctgcgg actcggccgc gtcgacgtgc
     1141 tcctcgagct ggcggaccat cgccgtgtgc tcctcgctct gggcgatgag ggcgtcgacc
     1201 cgggtgcgcg tcgaggtggc cgcggcctgg aggctcgatg agtcgagggc cagcccgctg
     1261 accgcggcca ggctctcgag gagggcggcc gccgcggccg ggtacggcat cccggacagg
     1321 tagtggggga cccgggccca gatgccgacg gcggggatgc cggcctgccc gagggcggcc
     1381 tcgatcggcg cgtggacccc cgccgggacc tcgatggtgc cgctgacgta cccgacggtc
     1441 ccggccagct cgggggaggt ggccgtggcc gccacccggg tgggacgggt gtgcggcacg
     1501 ggggccggga aggcccccag gcccacgacc aggcggacgt ccagggaccg ggccaggtcg
     1561 accaccgcgt ccgtgaaggc gtgccagcgc atgtcgggct cgggccccac gagaatcagg
     1621 gcggccttgc tgttggcgtc ctcgccgacg cgcagttcga tctctggcca cgtgacccgg
     1681 tcgatgacgc cgtcgatcag gcggagggtc ggccggcggg cccggtggtc gatcagctcg
     1741 tccgggtcga aggtggccat gagctcggtg gagatctgct ccagcacgtt ggccaccgca
     1801 gccttggcgc cgagcccggc gtcgatccag ccgtcgaggg cgaccaccat gaccggctcg
     1861 gacgggtggg tgtcgaggtg gcggacgaga agcgtcaagg gatcacagcc cggcggccgc
     1921 ggcccgggcc gactcggcca gccgctcgac ctgctggccg aagccctcgt agccgcttcg
     1981 gtcaccgccg ccggcgccgc ccaggtaccg ggacatgacc ccttcgagga tgcaggccag
     2041 cttccagtac ccgaaggcca cgtagaagtc gagggccgag aggtcgcggc ccgacctctc
     2101 ggcgtagcgg gctcgcagct ccgcccgggt cgggaagccg ggcagggcgg tgggggccac
     2161 gccgagcgca gggtcgcgat cgccgtcctc ggtccaatag accatcagga ggcccacgtc
     2221 ggcgagggga tcgccgaggg cgcagatctc ccagtcgagg accgcctgca ccgtgccgtc
     2281 gtcgcccacc atggtgttgt ccagccggta gtcgccgtgg acgatggtgg ccggcccctg
     2341 gtccgggatg cgcgccgcca ggacgtcgtg gacctcgtcg atggcgggga ccggccgtcc
     2401 gacgagcggg ttggacgact ggaactgggc ataccaccgc ttgagctggc gcgcgatgta
     2461 gccctcccgc cgcccgaggt cgccgagccc gaccgcgtcg acgtcgacgg catggatgcc
     2521 ggccagcacg tcggccaggt cctggccggc gcggcgccgc tgctcgacgt cgagaacttt
     2581 ctccaccatg gcagcgtctc gcagcacgtg gccctcgacg tagcccatca cgtagaaggg
     2641 gcgctggttg accgactcgt cggtgcagag cccgagcgcg ggcgggactg gaactgccgt
     2701 cggcccgagg caggagatga tccggtgctc acggaccatg tcgtgggcgg tgggcaggag
     2761 gtggctgacc gggggccggc gcaggacgta gtcgtgcccg gcggcgtcgg cgacccggaa
     2821 cgtgaggttg gagcggccgc ccgcgaccag ctcgaacgac agcggcgggg ctgccccctc
     2881 gatgttggcc tcgaaccagc gcgtgacgcg tgcctcgtcg atcgcctcca tagctgggga
     2941 agcctaatgg gtaggttgga gtgatgaccg acgtcaccga tgccagcttc gagcaggacg
     3001 ttctcgcccg gtcggacgag gtgcccgtcg tcgtggacct gtgggccccg tggtgcgggc
     3061 cgtgcaagac gctcggtccc atcctggagc gggtcgtcgg cgcgaccgac gggcaggtcg
     3121 acctggtcaa ggtcaacgtg gacgacaatc ccaacatctc ggccgccttc cgggtgcagt
     3181 ccatcccggc cgtctacgcc atcgccaagc gccaggtggt ggacagcttc gtcggcgcgc
     3241 tccccgagcc ggccgtgcgg gagtgggtca gccggctggc gccgccgccg agcgaggccg
     3301 accggctgac cgccaagggc gacgaagcct cgctgcgcca ggcgctcgag atcgatcccg
     3361 gacaccaggc cgcggtggag aagctggccg agctcctggt cgaccagggc caggccgagg
     3421 aggcgctggg cctgctcgcc cgcgtgcccg agacggccga tacccggcgc atcggcgcgc
     3481 tggcccggct
//
LOCUS       DASWGG010000007         4265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_327413, whole genome shotgun sequence.
ACCESSION   DASWGG010000007 DASWGG010000000
VERSION     DASWGG010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327413"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VF954_00125"
     CDS             complement(<1..1496)
                     /locus_tag="VF954_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529509.1"
                     /translation="MDLGGSWRAYPADDELRRVFADPAFDDESWETVRVPGHWRSAPA
                     FAASDGPVLYRRAFACDRPADDERVWLTFEGTFYLADAWLDGAYLGNSEGYFFPHTFE
                     ITDQVAARREHLLAVEVACEPPRDLTAKRNVTGVFQHWDCIDPASNPGGLWRPVGVGR
                     TGPVRLSRLRVLCVEATPERATVELAAALDAATAAEATITTTVAGAAGPALMERGDPH
                     HLAAGENLVRWRVTLERPELWWPHALGEQPLYDVSVAVDLGGARSDGREVRLGLRQVR
                     QRHWIFTVNGERLFVKGANYGPTRMAIGEARPAEIEADLQLARDAGLDLLRVHGHIAG
                     PDLYDAADRYGMLLWQDFPLQWGYARSARREAVRQATETVDLLGHHPSIVLWCAHNEP
                     FAVDHVAGDSHQPGGARLAARHGLPTFNKTVLDSSIRRALERADPTRPVIAHSGVLPG
                     LASSDSDSHLYLGWYGGAERDLPRWLERVPRLGRFLSEFGAQAVPASD"
     gene            1555..2955
                     /locus_tag="VF954_00130"
     CDS             1555..2955
                     /locus_tag="VF954_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEX9529510.1"
                     /translation="MSSRPATLGQLRESGWQSVPVKEEIRRNAVARIASGEPLVEGIL
                     GYEDTVLPQLENALLAGHDLIFLGERGQAKTRIIRSLAELLDEWLPIVAGSEINDDPY
                     HPVSRYARDQVADKGDDVPVEWVHRDTRYGEKLATPDTSIADLIGEVDPIRVAEGRYL
                     SDELTLHYGLVPRTNRGIFAINELPDLAERIQVGLLNVLEERDVQIRGYKIRLPLDLF
                     LVASANPEDYTNRGRIITPLKDRFGSQIRTHYPLEVGTELAVVRQEARPLTGEGLNVH
                     VPDFMTEIVGTLSHLARSSPKINQRSGVSVRLSIANFETLVANAARRALRAGERDVVP
                     RVSDLEALAASTAGKVEIETLDDGRDEDVVERLLKAATLTVFKERCPMDKLREVVGSF
                     DEGVIVHTGDDVASSSYVETLAALPALRPHVLSLAGSESPAAVASAMEFVLEGLHLSK
                     RLNKDSAGGRATYRAR"
     gene            2988..>4265
                     /locus_tag="VF954_00135"
     CDS             2988..>4265
                     /locus_tag="VF954_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529511.1"
                     /translation="MPLRYRYSRWDGTQVGFELDASDVLAEITDDLLYHGDLNAALRR
                     MLQSGFQDRNGERVAGMRELLERLRRRRRDELERYDLGGVYDDIAQELRDVIDTERGG
                     IDQLEQEAVASGDERRREITDQVTADKRLQLDMLPPDLAGQVRELQSYEFTSSEARER
                     FDELMDKLRQQLMQSYFNQMSGAMSDMSAEQVQRMKDMFNGLNQLLEMREQGQDTTAA
                     FDQFMQQFGDMFPGNPQTLEELLEQMAEQMAAMQAMLNSMTPEQRAQLQGLAEALLED
                     MDLRWQVDRLSNNLRNAFPGAGWDRRYQFSGQDPLGFAEAASLMDRLGDIDQLENLMR
                     SAVSPGALADVDLDRARDLLGNDGAASLERLAELAKMLEEAGLIEQREGRFELTPRGM
                     RRIGQNALADLFSKLAKDRMGRHEVDPSGVGHER"
BASE COUNT          650 a         1556 c         1503 g          556 t
ORIGIN      
        1 tcgtcgctcg cgggcacggc ctgcgcgccg aactcgctga ggaaccggcc caaccgcggc
       61 acccgctcca gccaccgcgg caggtcccgc tcggcgccgc cgtaccagcc gaggtacagg
      121 tggctgtcgg agtcggatga cgccaggccg ggcagcacgc cggagtgggc gatgaccggc
      181 cgggtcgggt cggcccgctc cagggcccgc cggatcgagc tgtcgagcac ggtcttgttg
      241 aaggtgggca gcccgtgcct ggccgccagc cgggcgccgc cgggctggtg tgaatcgccg
      301 gcgacgtggt cgacggcgaa gggctcgttg tgggcgcacc acaggacgat cgaagggtgg
      361 tggccgagca ggtcgaccgt ctcggttgcc tgccggacgg cctcccggcg ggcgctgcgg
      421 gcgtagcccc actggagggg gaagtcctgc caaagcagca tgccgtagcg gtcggcggcg
      481 tcgtagaggt ccggcccggc gatgtggccg tgcacgcgca gcaggtcgag gccggcgtcc
      541 cgggccagct ggagatcggc ctcgatctcg gccggccggg cctcgccgat cgccatgcgg
      601 gtcggcccgt agttggcgcc cttgacgaag agccgctcgc cgttgaccgt gaagatccag
      661 tgccgctggc gcacctgccg cagcccgagg cgcacctctc gtccatcgct gcgggcgccc
      721 cccaggtcga cggccaccga cacgtcgtag aggggctgct ccccgagggc gtggggccac
      781 cacagctcgg ggcgctccag cgtcacccgc caacggacca ggttctcgcc ggcggccagg
      841 tggtgcgggt ccccccgctc catcagggcc ggaccggccg ccccggcgac ggtcgtggtg
      901 atcgtcgcct cggccgcggt ggccgcgtcg agcgcggccg ccagctccac cgtggctcgc
      961 tccggcgtgg cctcgacgca cagcacccgc aaccggctga gccgcaccgg gcccgtgcgc
     1021 cctacgccga ccggccgcca cagcccgccg gggttggacg ccgggtcgat gcagtcccag
     1081 tgctggaaga cgcccgtcac gttgcgcttg gccgtgaggt cccgaggggg ctcgcaggcc
     1141 acctccaccg cgaggaggtg ctcgcgccgg gccgcgacct gatcggtgat ctcgaaggta
     1201 tggggaaaga agtagccctc gctgttcccc aggtaggccc cgtccagcca cgcgtcggcc
     1261 aggtagaacg tgccctcgaa ggtcaaccac acccgctcat cgtccgcggg ccggtcgcag
     1321 gcgaaggcgc ggcggtacag gaccggcccg tccgaggccg cgaaggcggg cgccgagcgc
     1381 cagtgaccgg ggacccggac ggtctcccag ctctcgtcgt cgaacgccgg gtcggcgaag
     1441 acgcggcgca gctcgtcgtc agcggggtag gcgcgccagc tcccgccaag gtccacgacc
     1501 cgccacccta gttcggctgg ggccgtaccc ctgggggcgc gtaggctctg ccccatgagc
     1561 tcgcgacctg caaccctggg acagctgcgg gagtccggat ggcaatccgt gcccgtcaag
     1621 gaagagatcc ggcgcaacgc cgtggccagg atcgcgtcgg gggagccgct ggtcgagggc
     1681 atcctgggct acgaggacac cgtgctgccc cagctggaga acgccctttt ggccggccac
     1741 gacctgatct tcctgggcga gcggggccag gccaagaccc ggatcatccg ctccctcgcc
     1801 gagctcctgg acgagtggct gccgatcgtg gccggctcgg agatcaacga cgacccctac
     1861 cacccggtgt cccgctacgc ccgcgaccag gtcgccgaca agggggacga cgtgccggtc
     1921 gagtgggtgc accgcgacac ccgctacggc gagaagctgg ccacccccga cacgtcgatc
     1981 gccgacctga tcggcgaggt cgaccccatc cgggtcgccg agggccggta cctgtccgac
     2041 gagctgaccc tgcactacgg gctggtgccc cgcaccaacc gggggatctt cgccatcaac
     2101 gagctgcccg acctggccga gcgcatccag gtcggcctcc tcaacgtgct cgaggagcgc
     2161 gacgtccaga tccggggcta caagatccgc ctgcccctgg acctgttcct cgtggcctcg
     2221 gccaaccccg aggactacac caaccggggc cgcatcatca ccccgctcaa ggaccggttc
     2281 ggctcgcaga tccggaccca ctacccgctc gaggtcggca cggagctggc cgtcgtgcgc
     2341 caggaggccc gcccgctcac cggcgagggg ctcaacgtgc acgtccccga cttcatgacc
     2401 gagatcgtcg ggaccctctc ccacctggcg cgctccagcc ccaagatcaa ccagcgctcc
     2461 ggggtgtccg tgcgcctctc catcgccaac ttcgagacgc tggtggccaa cgccgcccgg
     2521 cgagcgctgc gcgccggcga gcgcgacgtc gtgccccggg tcagcgatct cgaggccctc
     2581 gccgcctcca ccgccgggaa ggtcgagatc gagaccctcg acgacggccg cgacgaggac
     2641 gtggtggagc gccttctcaa ggcggccacc ctcaccgtct tcaaggagcg ttgcccgatg
     2701 gacaagctcc gcgaggtggt gggctccttc gatgaggggg tcatcgtcca caccggcgac
     2761 gacgtggcct cgtcgtcgta cgtcgagacg ctcgccgccc tgcccgccct gcgaccgcac
     2821 gtcctgagcc tggccggctc ggagagcccg gccgcggtgg ccagcgccat ggagttcgtc
     2881 ctcgagggcc ttcacctctc gaagcggctc aacaaggact ccgccggcgg gagggcgacc
     2941 taccgcgcca ggtagggccc gcccgggcca gggcgtagcc tgcaggcatg cccctccggt
     3001 accgctactc ccgctgggac ggcacccagg tcggattcga gctcgacgcc tccgacgtgc
     3061 tggccgagat caccgacgac ctgctctatc acggcgacct caacgccgcc ctgcgccgca
     3121 tgctccagtc cggcttccag gaccgcaacg gcgagcgcgt cgccggcatg cgcgagctgt
     3181 tggagcggct gcgccgccgc cgccgggacg agctggagcg ctacgacctg gggggcgtgt
     3241 acgacgacat cgcccaggag ctgcgggacg tgatcgacac cgagcggggc ggcatcgacc
     3301 agctcgagca ggaggcggtg gcgtccgggg acgagcgccg ccgggagatc accgaccagg
     3361 tgacggcgga caagcggctc cagctcgaca tgctcccgcc cgacctggcc ggccaggtgc
     3421 gtgagcttca gagctacgag ttcacctcgt ccgaggcccg cgagcgcttc gacgagctca
     3481 tggacaagct gcgccagcag ctcatgcaga gctacttcaa ccagatgtcc ggggccatgt
     3541 ccgacatgag cgcggagcag gtgcagcgca tgaaggacat gttcaacggg ctcaaccagc
     3601 tgctcgagat gcgcgagcag ggccaggaca cgaccgccgc gttcgaccag ttcatgcagc
     3661 agttcggcga catgttcccg ggcaaccccc agacgctgga ggagctgctg gagcagatgg
     3721 ccgagcagat ggcggccatg caggcgatgc tcaactccat gacgcccgag cagcgggccc
     3781 agctccaggg tctggccgag gcgctgctag aggacatgga cctgcgctgg caggtcgacc
     3841 ggctgtccaa caacctgcgc aacgccttcc cgggcgcggg gtgggaccgg cgctaccagt
     3901 tcagcggcca ggacccgctg ggcttcgccg aggccgcctc gctgatggac cgcctcggcg
     3961 acatcgacca gctcgagaac ctcatgcgct cggcggtgtc acccggcgcc ctcgccgacg
     4021 tcgacctgga ccgggcccgc gacctgctcg gcaacgacgg ggcggcctcg ctcgagcgcc
     4081 tcgccgagct ggccaagatg ctggaggagg ccgggctcat cgagcagcgg gagggccgct
     4141 tcgaactgac accgcgtggg atgcggcgca tcggccagaa cgcactggct gaccttttct
     4201 ccaaacttgc gaaggaccgg atggggcgcc acgaggtgga cccgagcggg gtgggccacg
     4261 agcgc
//
LOCUS       DASWGG010000008         3655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1300027, whole genome shotgun sequence.
ACCESSION   DASWGG010000008 DASWGG010000000
VERSION     DASWGG010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300027"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VF954_00140"
     CDS             complement(<1..266)
                     /locus_tag="VF954_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529512.1"
                     /translation="MSRPAVVVVAVVAGRVGTVIGDVIGAGTGAKPPSVLSRVETPGV
                     TPPGSGTGKPAGGAEPGLPVGLSWVAAIGPLAAPPAAGAGAAAGA"
     gene            complement(1427..1500)
                     /locus_tag="VF954_00145"
     tRNA            complement(1427..1500)
                     /locus_tag="VF954_00145"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1464..1466),aa:Val,seq:tac)
     gene            complement(1528..1965)
                     /locus_tag="VF954_00150"
     CDS             complement(1528..1965)
                     /locus_tag="VF954_00150"
                     /inference="COORDINATES: protein motif:HMM:NF016356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEX9529513.1"
                     /translation="MKPPLLVSACLLGVACNHRGRSSPSPAVARLGATHRLVPVCPEA
                     AGGLPTPRPAAELHADGSVRTEAGDDVSDAYRRGAAAAVALAAALGATEAVLKARSPS
                     CGCHQTYDGSVRGRLVSRPGVTAAALAAAGLAIRSEEDLQTEP"
     gene            complement(2081..2395)
                     /locus_tag="VF954_00155"
     CDS             complement(2081..2395)
                     /locus_tag="VF954_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX9529514.1"
                     /translation="MLAESSEEELNPIVEQPVRWLDARCRDGAASMTALFFSEQLDDI
                     ARAKAICGLCPVIEPCLRGAIDRHEPWGVWGGQLFFNGRILAFKRKRGRPPKNQAAQL
                     TA"
     gene            complement(2540..2740)
                     /locus_tag="VF954_00160"
     CDS             complement(2540..2740)
                     /locus_tag="VF954_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529515.1"
                     /translation="MKLTAWYAEKPAGVSGSGARQRGLTGTPHATAFAHRPVCIADAV
                     KAPKGANGMAGRPKAPPGGWSG"
     gene            complement(2815..3594)
                     /locus_tag="VF954_00165"
     CDS             complement(2815..3594)
                     /locus_tag="VF954_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX9529516.1"
                     /translation="MDDESLVAVLVDAAGAVARSLDGLEDWGLAGTRPWQYRSDLAAD
                     DAALAVLHHAGLAVLSEESGLTGPEHPILVVLDPVDGSTNAARGVPWFATSLCALDAD
                     GPRAAVVVNQASGDRYDAVRGCGARRNGAPLRPSPATEVGESMLALSGYPPRHLGWRQ
                     YRALGASALDLCAVASGVVDGFVDCSRDAHGPWDYMGGLLVCTEAGASVIDADGRDLV
                     VREHAARRTPLAAGTPELLAALRARRAGMLGGSLKTGEEIS"
BASE COUNT          544 a         1359 c         1253 g          499 t
ORIGIN      
        1 gcgcccgcag ccgcgcccgc gcccgcggcc ggcggagcgg caagcgggcc gatcgccgcc
       61 acccacgaca gcccgacggg cagcccgggc tcagcccccc cggccggctt gcccgtccct
      121 gaccccgggg gcgtgacacc gggcgtctcg accctgctca acacgctcgg cggcttggcc
      181 ccggtccccg cgccgatcac gtcgccgata acggtcccga ctctcccggc cacgaccgcc
      241 accaccacca cagcaggcct gctcacaccc tccggcctcg gccttcccac cacctgcgtc
      301 aaggtcggcc cggtctccac gtcgaactgc tgacaccggg tcggggttcg ggcggagtgg
      361 gccaccaccc gcctcgttca gaagacgctc aggctttctt aacaggccat gctctcaagt
      421 gctggggcgc cgaagtcgac gcctttcggg atgtcggcca gcccggcacg tccctacacc
      481 cgccgaggtc gcggcctaca tcgaagacta cgaacgcgcc cgcggcgccg ccttctcatc
      541 ccgagagaca gtgagcagtg agcagacggt cgagtctagt gctacgatgt agcacatgga
      601 acgcatagga gtacgagagc tccggcagca cgcaagtcgc tatctcgagc gcgtggtccg
      661 cggcgaatca ctggaggtta cggaccgcgg ccggcccgtg gcacgtctcg tgccggttcg
      721 cgccgaggcc tgggcagaca tggtcgcgag tgggagggtc acgccagcag acgacgtcgc
      781 cgacgtcgcc gacgagcccc cgggcgacta cgacgtcgaa ccttctgcag ttctcggggc
      841 aatgcgcgct gaagagagat gaagctctac ttggactcgt cggccctggt gaagctcgtg
      901 agacaagagg cagagtcggg cccgttgaga cgtttccttc gccgtcatcg ggccgacggc
      961 cgggtcacga gcgcactcgc cagagtggag gttgtgcgcg ccgtaactgg aggtggctct
     1021 gctgcagttg tccacgcgag gaggcagctc gctcgcgtgg accagatcaa tctcgaccga
     1081 cagctgctcg atgaagcggc cgctctggcg ccagggaccg tcctgcgctc gctcgacgca
     1141 atccacctgg cgtctgcgag ggcagtcggt tctgacctac gcgccctagt cagctatgac
     1201 ctgcgactga acgctgcggc cgccgtgctt ggcatcgcgg tggacgctcc cacctgaccg
     1261 gttagttgtc atgccctgcg gagcaagatt cgccgagcaa tgcaaatcac tgaattggac
     1321 tggcccgtcg gagctactcc tcggggcacg ccgccggaga gacaaagcgt gttcgtgccg
     1381 tgccaccttc tggcggacga agccgcaggt caaacagcgg gtcgggtggg cgttgaggga
     1441 ctcgaacccc cgacccctgc cttgtaaggg cagtgctcta gccaactgag ctaaacgccc
     1501 gcggtcccca cggtaacgga cgcccgctca aggctcggtc tggagatcct cctccgaacg
     1561 gatggccagc cccgccgcgg ccagcgcggc cgcggtcacg cccggccggg aaacgagccg
     1621 cccccggacc gacccgtcgt aggtctggtg acagccacag gagggcgagc gggccttcag
     1681 caccgcttcg gtcgcgccga gtgccgcggc cagcgccacc gccgccgccg cgcctcggcg
     1741 gtaggcgtcg gagacgtcgt ctcccgcctc cgtccgcacc gacccgtcgg cgtgaagctc
     1801 ggccgccggc cggggggtcg gcagcccccc ggcggcctcc gggcagaccg gcaccagtcg
     1861 gtgggtggcg ccgagccggg cgaccgccgg cgacgggctg gaccggccgc ggtggttgca
     1921 cgccacgccg agcaggcacg ccgacaccag cagcggtggc ttcaccgtag agacgctcgt
     1981 cgaggcgggc atcgatcgtc aagccacctc tggccccgcc catcatcgag ccgccaacgg
     2041 cgggcccgga cccgggggag agggccgggc ccgcgccggg tcaggcggtg agctgcgccg
     2101 cctggttctt cggcggccgg ccgcgcttgc gcttgaacgc gaggatcctg ccgttgaaga
     2161 acagctggcc gccccagacc ccccacggct cgtggcggtc gatggcgccc cgcaggcacg
     2221 gctcgatcac cgggcagagc ccgcagatcg ccttggcccg ggcgatgtcg tcgagctgct
     2281 ccgagaagaa cagcgccgtc atgcttgcag ccccgtcccg gcagcgagca tcgagccagc
     2341 gcacgggctg ctcgacgatt gggttgagct cttcctcact gctctcggcg agcatctcac
     2401 ctctcccctc ttccttgacc ggagccccgt tgccccggtc gggcactgca tgagcaaacc
     2461 tggcgaagca aacctgggaa acaaaaaggc cgccggggcg tcccggcggc ctgtggccct
     2521 tcctgttgct ggcgggttct cacccagacc agcctccagg aggagccttc ggccgcccgg
     2581 ccatgccgtt ggcgcccttg ggcgccttga cggcatcagc gatgcacacc ggacggtggg
     2641 caaaggcggt ggcgtgcggc gttccggtga gcccgcgctg gcgcgcgccg ctgccggaga
     2701 cgcccgcagg cttctcggcg taccaagcgg tgagcttcac gcgttctcct cgacgtccag
     2761 ccgccccggg cagatcccga agccctctta tcgcaacacg ccgcgagggt gacgtcaact
     2821 gatttcttcc ccggtcttca acgagccccc gagcatcccg gctcgccggg cacgcagggc
     2881 agccagcagc tccggcgtgc cggccgcgag gggcgtccgc cgggccgcgt gctcccggac
     2941 cacgaggtcg cggccgtcgg cgtcgatcac cgatgcgccc gcctcggtgc acaccagcag
     3001 cccgcccatg tagtcccacg gcccgtgggc gtcgcggctg cagtcgacga acccgtcgac
     3061 caccccagaa gcaaccgcgc acaggtcgag cgccgacgct ccgagggcac ggtactggcg
     3121 ccagccgagg tggcgcggcg ggtagcccga gagggcgagc atcgactccc ccacctcggt
     3181 cgccgggctg ggccggagcg gtgcgccgtt gcggcgagca ccgcagccgc ggacggcgtc
     3241 gtaccggtcg cccgaggcct ggttgaccac gaccgcggcg cgcgggccgt cggcgtcgag
     3301 ggcgcagagg ctggtggcga accacgggac gccgcgcgcc gcgttggtgg acccgtccac
     3361 cggatcgagc acgaccagga tgggatgctc cggcccggtc agccccgact cctcggacag
     3421 caccgccagc ccggcgtggt gcaggacggc gagggcggcg tcgtccgcgg ccaggtcgct
     3481 gcggtactgc cacgggcggg tccctgccag cccccagtcc tccagcccgt cgagcgaccg
     3541 ggcgacggcg ccggccgcgt cgaccagcac cgccacgagg ctctcgtcgt ccaccacctc
     3601 ggaccctacc ggctggctca ttaggctggg ggccgtggcc gtcatcgatg tcgcc
//
LOCUS       DASWGG010000009         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2588389, whole genome shotgun sequence.
ACCESSION   DASWGG010000009 DASWGG010000000
VERSION     DASWGG010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2588389"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VF954_00170"
     CDS             <1..901
                     /locus_tag="VF954_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX9529517.1"
                     /translation="VHVRDVRSQLTARAVESYVAGGGIGVVQLLAGANGNDLALRRHY
                     VDTISGQEREAIDALREAQQNLAAKQTALRTDQHAAGLAVDLVSQTEAAAAAVDRQDR
                     TLLKGVQGDIAQLVAQQQAAEAAATAARVQAELQAREATQAASRAAAAARSTSAASPF
                     APSRGQASGAGTSPGGSDYGPVPAGAGGAVEEAKRQLGKPYAYGGAGPDSYDCSGLTM
                     WAWGHAGVSLPHSAADQYSVTTHIPVSALQPGDLVFYGSPPHHVGIYVGSGQMIDALH
                     SGTNVEYDSIYMEGDLIGGGRVN"
     gene            913..1857
                     /locus_tag="VF954_00175"
     CDS             913..1857
                     /locus_tag="VF954_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9529518.1"
                     /translation="MRAATIVERRIVVTDHPDPVPGHGEILVAMRAAGLNAADLIQVM
                     GAYPAPPGSPQDIPGMEIAGEVVAIGAGVTRWQPGDRVMAVVGGGAQAARLVLHERLA
                     VPVPDGMTWEEAGGFPEAFTTAHDALFTQAGLAMGEGVLVSGAAGGVGTAGVQLAAAA
                     GARVTGSVRDPARRPDVASLGAEVIDPGELRHHGPFDVILELVGAPNMAANLDVLATH
                     GRIAVIGVGAGAAAEINLMALMTKRARIHGSTLRARPLEAKADAARRIEVSVLPLVAA
                     GRIRVPVTAAFPLERAGEAYERFRAGGKFGKVVLLNAE"
     gene            complement(1945..>2289)
                     /locus_tag="VF954_00180"
     CDS             complement(1945..>2289)
                     /locus_tag="VF954_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9529519.1"
                     /translation="TVGLVDGAFLAAMRPGSVIVNIARGSLVDEAALLAALDRGIPEV
                     ALLDVFGREPLPEDDPLWSHPRVVVTPHSSAVSLLRHDRAAAVFARNLQRWQAGAPLD
                     SEVTAADLGPIH"
BASE COUNT          311 a          858 c          861 g          259 t
ORIGIN      
        1 ggtccacgtc cgcgacgtcc gcagccagct caccgcccgg gcggtcgagt cctacgtggc
       61 cgggggcggc atcggcgtcg tccagctcct cgccggcgcc aacggcaacg acctcgccct
      121 gaggcgccac tacgtcgaca ccatctccgg ccaggagcgg gaggccatcg acgcgctccg
      181 cgaggctcag cagaacctgg cggccaagca gaccgccctg aggaccgacc agcacgcggc
      241 cgggctcgcc gtcgacctgg tgtcgcagac cgaggccgcg gctgccgccg tcgaccgcca
      301 ggaccggacc ctgctcaaag gcgtccaggg cgacatcgcc cagctggtgg cccagcagca
      361 ggcggccgag gcggccgcca ccgcggcccg ggtgcaggcc gagctccagg cccgggaggc
      421 aacccaggcg gccagccgcg cagcggccgc ggcccgcagc accagcgcgg cgtcgccgtt
      481 cgccccgtcc cggggccagg caagcggcgc gggcacctcg cccggcggct ccgactacgg
      541 accggtcccc gccggggccg ggggcgccgt ggaggaggcc aagcgccagc tcggcaagcc
      601 ctacgcgtac ggcggggcgg ggcccgacag ctacgactgc tccggcctga ccatgtgggc
      661 ctggggccac gccggcgtgt ctcttcccca ctcggccgcg gaccagtaca gcgtgacgac
      721 gcacatcccc gtgagcgcgc tgcagcccgg ggatctggtg ttctacggca gcccgcccca
      781 ccacgtgggc atctacgtcg gcagcggcca gatgatcgac gccctccact ccggcaccaa
      841 cgtcgagtac gactcgatct acatggaagg cgacctcatc ggcgggggcc gggtcaacta
      901 gcgacccgcc ggatgcgggc cgccaccatc gtcgagcgcc ggatcgtcgt aaccgaccat
      961 cctgaccccg tgcccgggca cggcgagatc ctggtcgcca tgcgcgccgc tggcctcaac
     1021 gcggccgacc tcatccaggt catgggcgcc tacccggccc cgccggggag cccccaggac
     1081 atccccggga tggagatcgc gggcgaggtt gtcgccatcg gagcgggcgt cacccggtgg
     1141 cagccgggcg accgcgtcat ggccgtggtc ggcggcgggg cccaggcggc gcggctcgtc
     1201 ctgcacgagc ggctggccgt gcccgtgccc gacggcatga cgtgggagga ggcgggcggt
     1261 ttcccagagg cgttcaccac cgcccacgac gccctgttca cccaggccgg gctggccatg
     1321 ggcgaaggcg tgctggtgtc gggcgcggcg gggggcgtcg ggacggcggg cgtgcagctg
     1381 gccgcggccg caggcgcccg ggtcacgggg tcggtgcggg acccggcccg ccgccccgac
     1441 gtggcctcgc tgggcgccga ggtcatcgac cccggcgagc tccgccacca cggccccttc
     1501 gacgtcatcc tcgagctggt gggcgcgccg aacatggccg ccaacctcga cgtcctcgcc
     1561 acccatggcc gcatcgcggt gatcggcgtg ggtgccggcg cggcggccga gatcaacctg
     1621 atggccctga tgaccaagcg ggcccgcatc cacggctcca ccctgcgcgc ccggcccctc
     1681 gaggccaaag ccgacgccgc ccgccgcatc gaggtctccg tactgcccct ggtggccgcg
     1741 ggccggatcc gggtgccggt gaccgccgcc ttcccgctcg agcgggccgg cgaggcctac
     1801 gagcggttcc gggcgggggg aaagttcggc aaggtcgtgc tcctcaacgc ggagtgaagc
     1861 cgggcctagt gaattggcca cggccgcggc ggggttgggg tccccgccgt gcggaggagg
     1921 ccggggttgg ggccccggcc gggcctagtg gattgggccc aggtcggcag ccgtgacctc
     1981 gctgtcgagc ggggcgccgg cctgccaccg ttgcaggttt cgggcgaaga ccgccgcggc
     2041 ccggtcgtgg cgcaacagcg agaccgccga gctgtggggt gtgaccacga cccggggatg
     2101 ggaccagagc gggtcgtcct cgggcagggg ctcgcggccg aagacgtcga gcagggcgac
     2161 ctccggaatg ccccggtcca gggcagccag cagcgcagcc tcgtccacga gcgagccccg
     2221 ggcgatgttg acgatgaccg agccgggccg catggcggcg agaaaggcgc cgtcgacgag
     2281 tccaacggt
//
LOCUS       DASWGG010000010         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1300894, whole genome shotgun sequence.
ACCESSION   DASWGG010000010 DASWGG010000000
VERSION     DASWGG010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300894"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..655)
                     /locus_tag="VF954_00185"
     CDS             complement(83..655)
                     /locus_tag="VF954_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529520.1"
                     /translation="MIQRPTVEPLDEPEGNRQRFVISPLEPGFGHTLGNSLRRTLLSA
                     IPGAAVTQVRFDDALHEFAVVPGVKEDITDIILNLKDLVVRSESPEPVIVRLDRRGPA
                     EVTAADIQTTADVEILDPDRHIASVNAKGRLALDLTIEQGRGYRPIDRATRSSTIGVI
                     PVDAIFSPVRRMVVIPSMERGTPRSGGVIR"
     gene            complement(828..1454)
                     /locus_tag="VF954_00190"
     CDS             complement(828..1454)
                     /locus_tag="VF954_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9529521.1"
                     /translation="MPRTTLAGRPGHNRGRTYPPEVLSPEEVERLVGAASARSSTGIR
                     ARALILTLQRGGLRLGEALALYPRDVDLARGTIRILRGRGDKARTVAIDPGAAAAIGR
                     WLDRRAHLGIGRRRPLFCTLAGTALYPQQVRATLTRLGERAGIDKRVHPHGLRHAHAA
                     HLDRSGVPITTIVRQLGHANLAVTSRYLQSLGQDEHLARVQTINWQSP"
BASE COUNT          209 a          510 c          576 g          219 t
ORIGIN      
        1 ccgcccgcac aagccacacc accgaagacc tctgccgacg actggcccaa cgccacgcag
       61 tcgacctcgc ccccacatcc gtctaacgga tcaccccgcc cgacctcggc gtcccacgct
      121 ccatcgacgg gatgacaacc atccgccgca ccggcgagaa gatggcgtcg acggggatca
      181 ccccgatggt cgaggaccgg gtggcccggt cgatcgggcg gtagccgcgg ccctgctcga
      241 tggtgaggtc gagggcgagg cggcccttgg cgttgaccga ggcgatgtgc cggtcgggat
      301 cgaggatctc gacgtcggcc gtggtctgga tgtcggccgc cgtcacctcg gccgggcccc
      361 gcctgtcgag gcgcacgatc accggctcgg gcgactcaga ccgcaccacg aggtccttca
      421 ggttgaggat gatgtcggtg atgtcctcct tcaccccggg gacgacggcg aattcgtgga
      481 gggcgtcgtc gaagcgcacc tgggtgaccg ccgcccccgg gatggccgac agcagggtcc
      541 ggcggaggct attaccgagc gtgtggccga aacccggctc gagcggggag atgacaaagc
      601 gctggcggtt gccctcgggc tcgtcgagcg gctcgaccgt cggacgctgg atcatcagca
      661 tggggtggcg gctcccggtc ttccccacca cgcctgcatc cccgcggggg gcgaaggccc
      721 gaaaagatag cggcgggcat cgccttccgg agccaggccc gccgccccgc cgccgcccgt
      781 cggagccctc tcgagccggg cggccgccgt ggccatgctg gcttccctca agggctttgc
      841 cagttgatgg tttggacccg ggcgaggtgt tcgtcttggc cgaggctctg caggtagcgg
      901 ctggtgacgg ccaggttggc gtggccgagt tgacggacga tggtggtgat gggcaccccg
      961 gagcggtcga ggtgggcggc gtgggcgtgg cggagtccgt gggggtggac gcgtttgtcg
     1021 atcccggccc gctccccgag gcgggtgagg gtcgccctga cctgctgggg atagagggcc
     1081 gtgccagcga gggtgcagaa cagcgggcgg cgccgtccaa tgcccaggtg ggctcgccgg
     1141 tcgagccagc ggccgatcgc agcggcggcg cccgggtcga tcgccacagt gcgggccttg
     1201 tcgcctctgc ctctgaggat ccggatggtg ccccgggcga ggtcgacgtc tctgggatac
     1261 agcgccagcg cctctcccag gcgcagcccc ccgcgctgga gggtgaggat gagggcccgg
     1321 gcccggatcc cggtgctgga ccgggccgag gcggcgccca ccaggcgctc cacctcttcg
     1381 gggctgagta cctcgggggg gtaggtgcgg ccccggttgt gaccgggccg cccggcgagc
     1441 gtggtccgag gcatctgggc agctcctgtg gcgctaaggg ggaaaagcgc cactgtactg
     1501 cagccacgac ggag
//
LOCUS       DASWGG010000011         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1940210, whole genome shotgun sequence.
ACCESSION   DASWGG010000011 DASWGG010000000
VERSION     DASWGG010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940210"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VF954_00195"
     CDS             complement(<1..356)
                     /locus_tag="VF954_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529522.1"
                     /translation="MLLAVAGLVAAGCGPSVIRAASRPPIEVVPVSVDLGPEHLPEAR
                     PADPRPLRVLLVGDGVMVDAVPGLVAAFASTGRALVTDGSRMGIGLTQDGGWGWSAQW
                     SAMVSASRPDVVVVMLG"
     gene            486..884
                     /locus_tag="VF954_00200"
     CDS             486..884
                     /locus_tag="VF954_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX9529523.1"
                     /translation="MRAAADDLERALAQAGHGRAAQWGAAMTEALGRLRDAFEHHVAV
                     TESDNGLFAEVVETAPRLAHRADQLRRDHEAIRQAVAGALSAVKGVRDEGIEQAREEV
                     VGLLGRVVRHRSRGAELVYEAWSVDIEAAD"
     gene            886..1287
                     /locus_tag="VF954_00205"
     CDS             886..1287
                     /locus_tag="VF954_00205"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX9529524.1"
                     /translation="MPPLRDVLLDYCQTRPAAIEEYPFGEDVAVFKVGGRMFALLPLV
                     GEAAEISLKCEPGLAEELRARHPAIRAGYHLNKQHWNTITLDGSLERDRVIELVDHSY
                     ERVVAGLSRAARQRLGIGGASEPGMPVGMED"
     gene            1292..>1707
                     /locus_tag="VF954_00210"
     CDS             1292..>1707
                     /locus_tag="VF954_00210"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9529525.1"
                     /translation="MAEITHRRVATNGIELHVAEAGQGRPVVLCHGFPESWYSWRHQL
                     PALADAGYRALAPDQRGYGDSSLPGEVTDYGIRALCEDLLGLLDDIGERRAIFVGHDW
                     GALIVWDLARLYPERVDAVVGVSVPFFRPRVPPTQL"
BASE COUNT          254 a          635 c          609 g          209 t
ORIGIN      
        1 cccaacatca ccaccacgac gtcgggacgc gaggccgaca ccatggcgga ccattgcgcc
       61 gaccatcccc acccgccgtc ctgggtcaag ccgatcccca tgcgcgatcc gtcggtcacg
      121 agcgcccggc ccgtcgaggc gaacgcggcc accagccccg gcaccgcgtc gaccatcacc
      181 ccgtcgccca ccagcaggac ccgcaacggc cggggatcgg cggggcgggc ctcagggaga
      241 tgctcgggcc cgaggtcgac cgagaccggg accacctcga tcggtgggcg cgatgcggcc
      301 cggatcacag acgggccgca gccggccgcg accaggccgg cgacagccaa cagcaccctc
      361 cgcggcaccc ccccggtcac aagggcccag gatatccgcc cgggcgatgc tggctacgct
      421 gtgacgtggc caacgcgcga gaaccgacgc cgccgctgga gagtgttcgg ctccaccgag
      481 ccggggtgcg cgcggcagcc gacgacctgg agcgggcgct ggcgcaggcc ggtcatggcc
      541 gcgccgccca atggggcgcg gccatgaccg aggcgctcgg acgtctccgg gatgccttcg
      601 agcaccacgt cgccgtgacc gagtccgaca acgggttgtt cgccgaggtc gtggagaccg
      661 cgccccggct ggcccatcgc gccgaccagc tgcgccgcga ccacgaggcc atccgccagg
      721 ccgtcgcggg cgccttgagc gccgtcaagg gcgtgcgcga cgaggggatc gagcaggccc
      781 gtgaagaggt cgtcggcctc ctcggtcgcg tcgtgcggca ccggagccgc ggcgccgagc
      841 tggtgtacga ggcctggtca gtggacatcg aggccgccga ctaacgtgcc gcctctgcgc
      901 gacgtcctgc tcgactactg ccagacccgg ccggcggcga tcgaggagta cccgttcggc
      961 gaggacgtag ccgtgttcaa ggtgggcggg cgcatgttcg ccctgctgcc cctggtgggt
     1021 gaggccgcag agatcagcct caagtgcgaa ccgggcctgg ccgaggagct gcgggcgcgg
     1081 cacccggcca tccgggctgg ctaccacctc aacaagcagc attggaacac catcaccctc
     1141 gacggcagcc tcgaacgcga ccgcgtcatc gagctggtcg accactccta cgagcgcgtc
     1201 gtcgcaggcc tgtcccgggc ggcccgccaa cggctcggga tcggcggcgc gtccgagccc
     1261 gggatgccgg tcgggatgga agactgacgg gatggccgag atcacccacc gccgcgtggc
     1321 caccaacggc atcgagctgc acgtggccga ggccggccag ggtcggccgg tggtcctgtg
     1381 ccacggcttc ccggagtcgt ggtactcgtg gcgccatcag ctgcccgccc tcgccgacgc
     1441 ggggtaccgg gccctggccc cggaccagcg gggctacggc gacagcagcc tccccggtga
     1501 ggtgaccgac tacggcatcc gggcgttgtg cgaggacctc ctcggcctgc tggacgacat
     1561 cggggaacgc cgcgccatct tcgtgggcca cgactggggc gcgctcatcg tctgggacct
     1621 ggcccggctg taccccgaac gggtcgatgc cgtcgtcggg gtgagcgtgc ccttcttccg
     1681 gccacgcgtc ccgccgacgc agctgta
//
LOCUS       DASWGG010000012         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_980806, whole genome shotgun sequence.
ACCESSION   DASWGG010000012 DASWGG010000000
VERSION     DASWGG010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980806"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..2147)
                     /locus_tag="VF954_00215"
     CDS             complement(210..2147)
                     /locus_tag="VF954_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX9529526.1"
                     /translation="MATPRSSSRTPDQIGRVLGGRYRLTGLVGTGASAHVFVAEDVTL
                     RRIVAVKTLHPALADDEAFLRRFRAEARAAAALNHPHIMRVFDWGEADDGPYLVLEHL
                     VGGSLRDLLDAGHRLSPSQALMIGLEAASALDYAHRRGLVHRDVKPANLLFDEEGRLC
                     VADFGLARALAESAWTEPDGVVLGTARYASPEQVQGSAVDGRADVYALALVLVEAVTG
                     QVPFAADTTIATLMARLHQPLNAPAAMGALGPALERAAAPDREDRFDAAGLARALDAA
                     AAQLPRPAPIPVGWGAEAQAAPPFGPAPEPGTDLTELGTAYRATRPAAPPAGPTYATT
                     TLAPQIATATSPVTPPRRRRWPRRLFISLATLVLAGAVAAGALVAFTLTRPRYPVPTL
                     VGLTQDKAAAALAAEHLHLRLAGQRYDELVPAGVIVDQSPAASHRARKGTSVAVHVSE
                     GPQPRAVPDLTGLSLAAATSKLQDAGFTVGEADTFSDAVAKGFVVSWSPKDGPQAKGA
                     KVVITLSSGYQPLPDVTQMTPEQAATALTNAGYTPATAQQFSDTVPSGQILNTAPPAG
                     APTQHGTTVTINVSKGPDTTTVPPGVIGVSVDQATALLQQAGLQLGQVFGPSKHGQVF
                     YSDPNPGTKVKRQSQISLYTF"
     gene            2280..2663
                     /locus_tag="VF954_00220"
     CDS             2280..2663
                     /locus_tag="VF954_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529527.1"
                     /translation="MSVALAVAAFAAVFAFQSHPEPAPVVRDVAVRAVSPTPGSFDVT
                     QDIIWIEIDPTYSGVIQRIDQTVIPEDQLQIIPNVVRISFDPRQAESATGRLAPGRHC
                     AVAQFWPTGTPPEAGRTYSWCFNSH"
BASE COUNT          351 a          943 c         1033 g          358 t
ORIGIN      
        1 cgccgccgcc gcccgctcgg cccgtacgaa ggcctccagc agagcctcgg cgtcgggacc
       61 agacacgcag tcagtctcta tggaggacag caccgagcgc agggccccgg tggcctggtc
      121 cacgatctcc acgagcatgt agtcagccta cacagggggt gtgacaacga catcggaggg
      181 catgagtctc cgcgggtcgc ctgcgaagat cagaacgtgt agaggctgat ctgggactgc
      241 cgcttgacct tggtgcccgg gttcgggtcg ctgtagaaca cctgcccgtg cttggagggg
      301 ccgaacacct gcccgagctg gagtcccgcc tgctggagca gggccgtggc ctgatcgacg
      361 ctcacaccga tgacaccggg cgggaccgtg gtcgtgtccg ggcccttgga cacgttgatg
      421 gtgaccgtcg tgccgtgctg ggtgggcgcg cccgcgggcg gggcggtgtt gaggatctgg
      481 ccgctcggca ccgtgtcgct gaactgctgg gccgtcgccg gcgtgtaccc cgcgttcgtg
      541 agggcggtcg cggcctgctc gggcgtcatc tgggtcacgt ccggcagcgg ctggtacccc
      601 gaggacagcg tgatgacaac cttggcgccc ttggcctgcg gcccgtcctt ggggctccag
      661 ctgaccacga agcccttggc cacggcgtcg gagaaggtgt cggcctctcc cacggtgaag
      721 ccggcgtcct ggagcttcga ggtggccgcg gccaggctga gcccggtcag gtcgggcacc
      781 gccctgggct ggggcccttc cgagacgtgg acggcgacgc tcgtgccctt tcgggcgcgg
      841 tggctggccg ccgggctctg gtcgacgatg acgccggcgg gcacgagctc gtcgtagcgt
      901 tggccggcga gccggaggtg gaggtgctcg gcggcgaggg cggccgccgc cttgtcctgc
      961 gtgagcccga ccagggtggg caccgggtac cgcgggcggg tcagggtgaa ggccacgaga
     1021 gcgccggcgg cgacggcgcc ggccagcacc agggtggcga gcgagatgaa cagccggcgg
     1081 ggccatcgcc ggcggcgagg gggggtgacg ggcgacgtgg cggtggcgat ctgtggggcg
     1141 agggtggtcg ttgcgtacgt cggcccggcg ggcggagccg ccggtcgggt ggctcggtac
     1201 gcggtcccca gctcggtcag gtccgttccc ggctcgggcg cgggcccgaa gggcggcgcg
     1261 gcctgggcct ccgctcccca tcccacgggg atcggcgccg gcctgggcag ctgggcggcg
     1321 gcggcgtcca gggcccgggc caggccggcc gcgtcgaagc ggtcctcgcg gtccggcgcc
     1381 gccgcccgct ccagggccgg ccccagggcc cccatggccg ccggggcgtt gaggggctgg
     1441 tggagacggg ccatcaacgt ggcgatggtg gtgtcggccg cgaacggcac ctggccggtc
     1501 acggcctcga ccaggaccag ggcgagggcg tacacatcgg cccgcccgtc caccgccgag
     1561 ccctggacct gctcgggcga cgcgtagcga gccgtgccca gcaccacgcc gtcgggctcg
     1621 gtccaggccg actcggccag ggcccgggcc agaccgaagt cggccacgca aagccggccc
     1681 tcctcgtcga agagcaggtt ggccggcttc acgtcccggt gcaccaggcc ccgtcggtgg
     1741 gcatagtcga gggcagaggc cgcctccagg ccgatcatca gcgcctgcga cggcgacagc
     1801 cggtggccgg cgtcgagcag gtcccggagg ctgccaccca ccaggtgctc caggacgagg
     1861 tagggcccgt cgtcggcctc gccccagtcg aagacgcgca ttatgtgggg gtggttgagg
     1921 gctgcggcgg cccgggcctc ggcccggaag cgccggagga aggcctcgtc gtcggccagg
     1981 gcgggatgga gggtcttgac cgcgacgatg cggcgcagcg tgacgtcctc ggccacgaac
     2041 acgtgcgccg acgcgccggt gcccaccagg cccgtgagcc ggtagcggcc gccaagcacc
     2101 cgaccgatct ggtcgggggt gcgcgaagag ctccgcggcg tcgccacggg ccgagggtac
     2161 cgggtgggct accccgggag atggcacacg agatggcaca gggaggccgg acgggcacaa
     2221 tggggcccat cgccgcgccc gcccagacat cccgactcgt caccccttac cggctcgcgg
     2281 tgagcgtggc cctggcggtg gcggccttcg cggcagtgtt cgccttccag tcccacccgg
     2341 agccggcgcc ggtcgtgcgc gacgtcgccg tccgggccgt gtcgcccacc ccgggcagct
     2401 tcgacgtcac ccaggacatc atctggatcg agatcgaccc gacctactcc ggcgtcatcc
     2461 agcgcatcga ccagaccgtc atccccgagg accagctcca gatcatcccg aacgtggtgc
     2521 gcatctcctt cgacccccgc caggcggaat ccgccaccgg tcgactggcg ccgggccgcc
     2581 actgcgcggt cgcccagttc tggcccaccg ggacgccgcc cgaggcgggc cgcacctaca
     2641 gctggtgctt caactcgcac tgagggtcac ctcggtggcg cgggc
//
LOCUS       DASWGG010000013         3559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_983934, whole genome shotgun sequence.
ACCESSION   DASWGG010000013 DASWGG010000000
VERSION     DASWGG010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983934"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VF954_00225"
     CDS             <1..618
                     /locus_tag="VF954_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX9529528.1"
                     /translation="RLGTRYAVVAVVSGRPAAFLRQQLAGANVAMSGLYGLERALGEG
                     PVHVHPDAARWRDVVERAAVAASGAAPARVTVERKGLSVALHWRVAPEAEVWALEFAA
                     DAARRSGLVAHPSRMAVELRPPLDADKGSAVAGLVGGVAAAAFLGDDVGDLPAFAAVS
                     RPGLYPARIAVRSPETPPALIAAADAVVSGPQAALEVLAWLAGPD"
     gene            complement(641..2023)
                     /locus_tag="VF954_00230"
     CDS             complement(641..2023)
                     /locus_tag="VF954_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX9529529.1"
                     /translation="MPTSDLVVVSNRGPVSFTHGPAGELVSHRGGGGLVSLMAPALGD
                     TDALWVSAAMTDGDRAAAAGARVEAEGFRLWPVSVDPEEYRQFYDVIANATLWFTHHG
                     LHDLPRRPRFDRRWHEAWEAYVAVNARFADAVAEVAADGATVLVQDYHLTLLGTRLAK
                     ERPDVRSVHFHHTPFADPRELRVLPDAVAEQVLTGLAGHGACGFHSRRWAAAFEACCR
                     EVLGQSPPTFVSAAASDAASLRAIASAPAAADARARLDEMAGDRLLLVRVDRIELSKN
                     LLRGFLAFEEALASSPGLRGRVVFGAFCYPSREGLIDYLAYRQEVEGLVARINETWGD
                     ADWTPIVLDETDDYPRSVAALARADAILVNPVRDGLNLVAQEAVLVNDRLAPLVLSHE
                     TGVWDQLGDGAIGINPFDVSATAEAIVAALEMPAHERQVRADKLRAIAGRRRPADWLA
                     DLVAAAGAPG"
     gene            2067..3050
                     /locus_tag="VF954_00235"
     CDS             2067..3050
                     /locus_tag="VF954_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX9529530.1"
                     /translation="MPHLVAAPDKFRGTATAAEVAAAMARAGVAAGWTVDQAPVADGG
                     EGILEVLGGAVRHTSVAGPLGQRVDAEWRMQGTTAVIEMAQASGLALAGGTEANDPMR
                     ASTAGTGELIAAAVAAGARKVIVGAGGSATSDGGLAALNVLRPFARLRGVELVVACDV
                     STRFVDAARVFAPQKGATPAQVALLTRRLERLAQVYEQDYGVDVRDLAGSGAAGGLAG
                     GLAAAGAVLMPGFDLVADAIGLPDRLAVADLVVTGEGFLDEQSFEGKAVGGVLQLAAE
                     AGVAAVVVAGQVFGEPRARAISLVERFGEERAMADTLRCIEEASSEALRLL"
     regulatory      3108..3205
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3212..>3559
                     /locus_tag="VF954_00240"
     CDS             3212..>3559
                     /locus_tag="VF954_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9529531.1"
                     /translation="MPFVEGLRCQECSRPYPAEALHVCEYCFGPLEVVYDYEAIAAHV
                     SPESIAAGPNTLWRYAALLPADPFAPVDLGDGYTPLVRADRLAAELGLGELWIKDDTA
                     NPTGSFKDRVVSVA"
BASE COUNT          441 a         1295 c         1347 g          476 t
ORIGIN      
        1 cggctgggca cgcggtacgc cgtggtcgcc gtggtatccg gccgcccggc cgccttcctg
       61 cgccagcagc tggcgggcgc caacgtggcg atgtccggcc tctacgggtt ggagcgggcc
      121 ctcggcgagg gcccggtgca cgtccacccg gacgcggcgc gctggcgcga cgtggtcgag
      181 cgggcggcgg tggcggcttc cggcgcagca cccgcccggg tgaccgtcga gcgcaaggga
      241 ctctcggtgg ccctgcattg gcgcgtcgcg cccgaggccg aggtctgggc cctcgagttc
      301 gcggccgacg ccgctcgccg cagcggcctg gtcgcgcacc cctctcgaat ggcggtcgag
      361 ctgcggccgc cgctcgatgc cgacaagggc tcggcggtgg ccgggctcgt gggcggcgtg
      421 gccgcggccg cctttctcgg tgacgacgtc ggcgatctgc ccgccttcgc cgcggtgagc
      481 cgccccgggc tgtacccggc gcggatcgcg gtccgctcgc ccgagacgcc gcccgccctg
      541 atcgccgcgg ccgacgcggt cgtctccgga ccgcaggccg ccctggaggt cctcgcctgg
      601 ctcgccgggc ctgactgatc agccggcgcc tcggtcccgc ctacccgggc gcgccggccg
      661 ccgccaccag gtcggccagc cagtccgccg gccggcgccg gccggcgatg gcgcggagct
      721 tgtcggcccg gacctggcgc tcatgggcgg gcatctcgag ggctgccacg atggcctccg
      781 ccgtggccga cacgtcgaag gggttgatgc cgatggcgcc gtcgccgagc tggtcccaca
      841 ccccggtctc gtgcgagagg accagcggcg ccaggcggtc gttgaccagc accgcctcct
      901 gggcgaccag gttgaggccg tcgcgcaccg ggttgaccag gatggcgtcg gcccgggcca
      961 gggcggcgac agagcgcggg tagtcgtcgg tctcgtccag cacgatcggg gtccaatcgg
     1021 cgtcgcccca ggtctcgttg atcctcgcga ccaggccctc gacctcctgg cggtaggcga
     1081 gatagtcgat gagcccctca cggctcggat agcagaacgc gccgaacacc acccggccgc
     1141 gcagcccggg tgagctagcc agggcctctt cgaaggccag gaagccccgc agcaggttct
     1201 tggacagctc gatccggtcg acccggacta acagcaaccg gtcgccggcc atctcgtcga
     1261 gccgggctct cgcgtcggcg gcggccgggg ccgaggcgat ggctcggaga gacgcggcgt
     1321 cgctggcggc ggccgagacg aacgtcggcg gcgactggcc caggacctcc cggcagcagg
     1381 cttcgaacgc ggccgcccac cggcgggagt gaaagccgca cgcgccgtgc ccggcgagcc
     1441 cggtcagcac ctgctcggcc accgcgtcgg gcagcacccg cagctctcgg ggatcggcga
     1501 agggcgtgtg atggaagtgc acgctccgaa cgtccggccg ctccttggcc aggcgcgtgc
     1561 ccagcagcgt caggtggtaa tcctgcacga gcaccgtggc cccgtcggcg gccacctctg
     1621 ccaccgcatc ggcgaagcgg gcgttcaccg ccacgtaggc ctcccatgcc tcgtgccagc
     1681 ggcggtcgaa ccgcgggcgg cgaggcaggt cgtggagccc gtggtgggtg aaccacaggg
     1741 tggcgttggc tatgacgtcg tagaactggc ggtactcctc ggggtcgacg cttaccggcc
     1801 acagccggaa gccctcggcc tccacccgcg cccctgccgc cgcggcgcgg tccccgtcgg
     1861 tcatggccgc cgacacccac agcgcatcgg tgtcgcccag cgccggagcc atgagcgaca
     1921 ccagcccgcc gccgccgcgg tggctcacca gctcgcctgc cgggccgtgg gtgaaggaca
     1981 ccggcccgcg gttggagacg acgacgagat cggaggtcgg catggcaccg gcaccctacc
     2041 caccacgacc gggcaccatg ggtgccgtgc cgcacctggt cgccgcgccg gacaagttcc
     2101 gggggaccgc caccgcagcc gaggtggctg ctgccatggc gcgggctggc gtcgccgcgg
     2161 gctggaccgt cgaccaggca ccggtcgcgg acggcggcga ggggatcctc gaggtgctgg
     2221 gcggcgcggt ccgccacacg tcggtggccg ggccgctggg ccagcgggtc gatgccgagt
     2281 ggcgcatgca ggggaccacg gcggtcatcg agatggctca ggcctccggg ctcgccctgg
     2341 ccggcgggac ggaggccaac gaccccatgc gtgccagcac cgccggcact ggtgagctga
     2401 tcgccgccgc ggtcgcggcc ggcgcccgga aggtgatcgt cggggccggc gggtcggcca
     2461 cgagcgacgg cgggctggcg gccctcaacg tcctgcggcc gttcgcccgg cttcgtggcg
     2521 tcgagctggt ggtggcgtgc gacgtgtcca cccgttttgt cgacgccgcc cgggtcttcg
     2581 caccccagaa gggggccacg cccgcccagg ttgcgctcct cacccgccgg ctcgaacgcc
     2641 tggcccaggt ctacgagcag gactacggcg tcgacgttcg tgacctcgcc ggctcgggcg
     2701 cggccggcgg gctggcgggc gggctggcgg cggccggggc cgtgctgatg ccgggcttcg
     2761 acctggtggc cgacgccatc gggctgcccg accggcttgc cgtcgccgac ctggtggtca
     2821 cgggtgaggg cttcctcgac gagcagagct tcgagggaaa ggcggtcggc ggggtgctcc
     2881 agctggcggc cgaggcgggg gtggcggcgg tggtagtcgc cggccaggtc ttcggagagc
     2941 cgagggcccg agccatctcg ctggtggagc gcttcggcga ggagcgagcc atggccgaca
     3001 ccctccgatg catcgaggag gcctccagcg aggcgctgcg cctcctctag ggccgatggc
     3061 accgccaagt gctttgacga gcacggcccg gctcggccac catggtgtca tcatccagag
     3121 cggtcgaggg acgtggcccg acgacaccgc ggcaaccagt gccggctccg ccttgcacct
     3181 ggtgccaatg cccgagcgat gaggaggagc tgtgccgttc gtcgaaggtc tccggtgcca
     3241 ggagtgctcg cgcccctacc cggctgaggc gctgcatgtg tgcgagtact gcttcgggcc
     3301 actcgaggtg gtgtacgact acgaggccat tgccgcccac gtctcgccgg agtcgatcgc
     3361 ggcgggcccg aacacgctgt ggcgctatgc cgcgctcctg cctgcggatc cgttcgcccc
     3421 ggtcgacctg ggcgacggct acaccccgct cgtgcgcgcc gaccgcctgg ccgccgaatt
     3481 gggactgggt gagctgtgga tcaaggacga cacggccaac cccaccggct cgttcaagga
     3541 ccgggtggtc tcggtggcc
//
LOCUS       DASWGG010000014         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1306639, whole genome shotgun sequence.
ACCESSION   DASWGG010000014 DASWGG010000000
VERSION     DASWGG010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306639"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VF954_00245"
     CDS             complement(<1..470)
                     /locus_tag="VF954_00245"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HEX9529532.1"
                     /translation="MPPLKVTVEAEVEVVALELRELEVTEPRADFTLEVEVATAAARA
                     GRVAETAPARALYRRFGIDPTKTRPSSEALLRRALRGDRFPRINSLVDVANVMSLLAQ
                     SPVGLYDLDRVEGLLTIRVGAEGEGYQGIGKDHVNVGGRLCVADAYGPCGNPSS"
     gene            complement(458..1723)
                     /gene="aroA"
                     /locus_tag="VF954_00250"
     CDS             complement(458..1723)
                     /gene="aroA"
                     /locus_tag="VF954_00250"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX9529533.1"
                     /translation="MTVAVRRPRRLEGVLALPGDKSVSHRALMLNAVAEGEAQVGNLS
                     PGADVASTAACLRDLGVDLDDGKVAGRGFAGLRAPAGPLDCGNSGTTMRLLAGLLAGR
                     PFESVLVGDASLSRRPMDRVVTPLRAMAAAAEAGPPLRVGGSVPLHGIDYELPVASAQ
                     VKSALLLAGIQATGATRVTEPAPTRDHTERMLEAMGAPVERAGNAISVRRADSLRPLD
                     IRVPADLSAAAFWLVAGALHPAARVRLRGVGVNPTRTAVLGVLRRAGLQVRLSDERME
                     GLEPVADLEVGSSPADRAFTLDGGEAALLIDELPVLAVAAALLPGISRVTGARELRVK
                     ESDRVSAMAEGLNAMGADVRELDDGWQIHGPRHLEGARVRSHGDHRVAMALAVAGLLA
                     DGETTIDDGECVAISYPGFWDDLDALCRR"
BASE COUNT          250 a          722 c          660 g          218 t
ORIGIN      
        1 tcggagctgg ggttgccgca ggggccgtag gcgtcggcca cgcacagccg gccgcccacg
       61 ttgacgtggt ccttgccgat gccctggtac ccctcgccct cggctccgac ccggatcgtg
      121 aggaggccct ccacgcgatc gaggtcgtag aggccgaccg gcgactgggc cagcagggac
      181 atcacgttgg ccacgtcgac cagcgagttt atgcggggga agcggtcgcc ccgcagcgcc
      241 cgccggagca gcgcctcgct cgaggggcgc gtcttggtgg ggtcgatccc gaaacgccgg
      301 tagagggccc gcgcgggggc ggtctccgcc actcgcccgg ccctggcggc cgccgtggcc
      361 acctccacct cgagggtgaa atcggcccgc ggctcggtca cctcgagctc gcgcagctcc
      421 agggcgacca cctcgacctc cgcctcaacg gtgaccttca gcggcggcaa agggcgtcca
      481 ggtcgtccca gaagccgggg taggagatgg ccacgcactc gccgtcgtcg atggtggtct
      541 cgccatctgc gagcaggccc gccaccgcca gggccatggc gacccggtgg tcaccgtgcg
      601 agcgcacgcg ggcgccctcc aggtggcgcg ggccgtggat ctgccagcca tcatccagct
      661 cgcgcacgtc ggcgcccatg gcgttcaggc cctcggccat cgcggacacc cggtccgact
      721 ccttgacccg cagctcgcgc gccccagtga cgcggctgat gcccggcagc agcgccgccg
      781 ccaccgccag caccggcagc tcgtcgatga gcagcgccgc ctcgccgccg tcgagagtga
      841 aggcgcgatc ggcgggcgac gacccgacct cgaggtccgc caccggctcc agcccctcca
      901 tccgctcgtc cgacaaccgc acctggaggc ccgcgcggcg cagcactccc agtacggcgg
      961 tccgcgtcgg gttgactccc acgccgcgca ggcgcacccg ggccgccggg tgcagggcac
     1021 cggccaccag ccagaacgcc gccgccgaga ggtcggccgg aacccggatg tccagcgggc
     1081 gcagggagtc cgcccgccga accgagatcg cattgcccgc gcgctccacc ggggcgccca
     1141 tcgcctccag catccgctcc gtgtggtcgc gggtcggtgc cggttcggtc accctggtcg
     1201 ctcccgtggc ctggatcccg gccagcagca gcgccgactt gacctgagct gacgcgaccg
     1261 gcagctcgta gtcgatcccg tgcagcggta ccgacccgcc gacccgcagg ggggggcccg
     1321 cctcggcggc ggccgccatg gcccgcagcg gggtcaccac gcggtccatg gggcgccggc
     1381 tgagcgaggc gtcccccacc agcaccgact caaagggccg tcccgcgagc aggccggcca
     1441 ggagccgcat ggtagtgccc gagttgccgc agtcgagcgg gcccgccggc gctcggagac
     1501 cagcgaagcc gcggccggcc accttcccgt cgtcgagatc gaccccgagg tcgcgcaggc
     1561 aggcggcggt ggaggcgacg tcggcccccg gcgagaggtt gcccacctgc gcctcgccct
     1621 cggccacggc gttgagcatc agcgcccggt gcgacaccga cttgtccccg ggcagggcca
     1681 gcacgccttc caggcgccgc ggcctacgga cggcgacggt caccggactc ccagcgccgg
     1741 cgggcgctgc gcgcctcttc gaagagctcg gccagccgag gatcgtcggg gtcgaggtgg
     1801 cgccggagac gcgccagcga ggcttcgaag cgttccaggg cctccgcgat
//
LOCUS       DASWGG010000015         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1306818, whole genome shotgun sequence.
ACCESSION   DASWGG010000015 DASWGG010000000
VERSION     DASWGG010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306818"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VF954_00255"
     CDS             complement(<1..1078)
                     /locus_tag="VF954_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9529534.1"
                     /translation="MTTWVIRLDEPRAPVAGPRVAVKDAIDIAGLPTSAGCRAVADRA
                     EPAEADAACLAGTRAAVGATIAGKTNLHELCFGTTGVNPWLGTPVNTRLPGRVPGGSS
                     SGSAAAVAAGEADVGIGTDTGGSVRIPAACCGIVGLKTTWGRVPLDGVWPLAPALDTV
                     GPLARTVAEAATGLSLLDPGFGGPSRPAAAVGRLRVPGVDPEIDAAIDGALAASELEV
                     HDIELPGWDATTAPFLVLLLFGAWRADGHLIGSPGVSPDIAARVLAGRDITDDQVATA
                     GLERAAWCRELATLFARAEVIALPTLDRFPPEVADAADAHLNRLTSPWNLSGSPAISL
                     PVPARGPLPASLQLAGPDLSEDVLC"
     gene            complement(1137..>1503)
                     /locus_tag="VF954_00260"
     CDS             complement(1137..>1503)
                     /locus_tag="VF954_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529535.1"
                     /translation="MTVTIKSTGGTGWAISMTNPRWTWALTLSYSSTFSSAEWILEAP
                     TVVVQTVLANVGTQKFTAGTPGNTFTVDGATKPIGQGNPVRILLSPGLINEATPSTLN
                     PSGTAFNDCAYQQSCNSPA"
BASE COUNT          202 a          504 c          593 g          204 t
ORIGIN      
        1 cacacaggac gtcctcgctt aggtcgggcc cggcgagctg gaggctggcg ggcagcgggc
       61 cgcgtgccgg caccggcagg gagatggcgg gcgacccgga gaggttccaa ggagaggtga
      121 ggcggttgag gtgggcgtcg gcggcgtcgg cgacctcggg cgggaagcgg tccagggtgg
      181 gcagggcgat gacctcggcc cgggcgaaca gggtggccag ctcacggcac cacgccgccc
      241 gttcgaggcc ggccgtggct acctggtcgt cggtgatgtc ccgcccggcg aggacccgcg
      301 ccgcgatgtc cgggctcacg cccggcgagc cgatgagatg gccgtcggcg cgccaggcgc
      361 cgaagagcag cagcaccagg aacggggcgg tggtggcatc ccagccgggc agctcgatgt
      421 cgtggacctc cagttcgctg gcggcgagcg ccccgtcgat cgcggcgtcg atctcggggt
      481 cgacgccggg cacacggagg cggccgaccg ccgccgcggg ccggctcggc ccgccgaagc
      541 ccggatcgag gagcgacagc ccggtggccg cctcggctac ggtccgggcc agcggcccga
      601 cggtgtcgag ggcgggggcg aggggccaga cgccgtcgag gggcacacgg ccccaggtcg
      661 tcttgagccc cacgatcccg cagcaggcgg ccgggatgcg gaccgagccg ccggtgtcgg
      721 tgccgatccc cacgtcggcc tccccagcgg cgaccgcggc agccgagccg ctcgacgagc
      781 cgcccgggac gcggccgggg aggcgggtgt tcaccggtgt acccaaccac gggttgacac
      841 ccgtagtgcc gaagcacagc tcgtggaggt tggtcttgcc ggcgatggtg gccccgaccg
      901 cggccctcgt cccggcgagg catgcggcgt cggcctcggc gggctcggcc cggtcggcca
      961 cggcccggca gccggccgag gtcggcaacc cggcgatgtc gatggcgtcc ttgaccgcca
     1021 ccctcggacc ggcgaccggg gccctgggct cgtccagtcg gatcacccag gtggtcatgg
     1081 ccgcgagcct aggaggcccg ggccatggca cgtttcaggt attgactccc gggccgttag
     1141 gccggcgagt tgcagctctg ctgataggca cagtcgttga acgcagtgcc ggacgggttc
     1201 agcgtggacg gcgtcgcctc gttgatcagc ccggggctca agaggatgcg gaccgggttt
     1261 ccctggccga tcggcttggt cgcgccgtcc acggtgaagg tgttgcccgg tgtgccggcg
     1321 gtgaacttct gggtgcccac gttggccagc accgtctgta cgacgaccgt gggagcctcg
     1381 aggatccact cggcggagga gaacgtcgat gagtagctca gggtgagtgc ccaggtccag
     1441 cgggggttgg tcatcgagat ggcccacccc gtaccgcccg tgctctttat ggtcactgtc
     1501 att
//
LOCUS       DASWGG010000016         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1308472, whole genome shotgun sequence.
ACCESSION   DASWGG010000016 DASWGG010000000
VERSION     DASWGG010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308472"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VF954_00265"
     CDS             complement(<1..377)
                     /locus_tag="VF954_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529536.1"
                     /translation="MAKAGEHTMIIERRGRSHQAHCRCGWSGHAWNELRPAEADAWHH
                     VFGESCIVDSGRATAAPAAALPTYELPSLDAAIERLVTRARALAQSSSPYGEQAVGEL
                     RRLSGGDRKLAHAAIDQLSELLRR"
     gene            514..1707
                     /locus_tag="VF954_00270"
     CDS             514..1707
                     /locus_tag="VF954_00270"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX9529537.1"
                     /translation="MAFTPELDRAGVLTQWAATGRSNDSDPRSAGATAARHAIAGDDP
                     KLLVVFASDAYDLGQLLAAIADVSQGVPTIGCSTAGEIAASGPGTSGVVVMALGGPGF
                     SVATEVGRADAVGLRLAGAKVAASVGRIQARPHNVLLLLSDGLGGDQQEVVRGAHSTL
                     GAEVPLVGGCAGDDMKMRRTYQFHGSEVLEAAVVGAAIGSEGPIGIGVEHGWRAVGEP
                     MVVTGSCDNRVVSLDEQPALDAYFERLDAPEDVRHDAEAFTRYAMTHPLGLSRRSGDE
                     VRFVAGANFADRSINCIAAVPQGSLAWIMEGDSASVLGATDVACQRALAGLGGRAPLG
                     LLAFDCIARKGVMGDDGVATEVERIAGYAGGAPVAGFYTYGEIARTRGVNGFHNQTLV
                     VVAFA"
BASE COUNT          241 a          686 c          685 g          267 t
ORIGIN      
        1 cgacggagca gctcggacag ctggtcgatg gcggcgtggg ccagcttgcg gtccccgccc
       61 gagagccgcc gcagctcgcc cacggcctgc tcgccgtagg gggacgagct ctgggccagc
      121 gcccgggccc gggtgacgag ccgctcgatg gccgcgtcca gggagggcag ctcgtaggtg
      181 ggcagggccg cggcgggtgc ggcggtcgcc cggccgctgt cgacgatgca gctctcaccg
      241 aacacgtggt gccacgcgtc ggcctcggcg gggcgcagct cgttccacgc gtgccccgac
      301 cacccgcacc ggcagtgggc ctggtgggac cgccctcgcc gctcgatgat catggtgtgc
      361 tcgccggcct ttgccaccca acctcccggt accacctgag actaccccgc gcggcgggca
      421 ggggacatgg cccggtggcc gcgctcgcgc tatggattgc tcatttcgcg ccacccgtct
      481 catgccggcc ccgatcggcg tcgaatggtc aacatggcgt tcacccctga gctggaccgc
      541 gcgggtgtgc tgacccaatg ggccgccacg gggcggtcca acgactccga cccccgctcg
      601 gccggcgcca cggccgcccg ccatgccatc gccggcgacg accccaagct gctggttgtc
      661 ttcgcatccg acgcctacga cctcggccag ctgctcgccg cgatcgccga cgtgagccag
      721 ggcgtgccga cgatcggctg ctcgaccgcg ggtgagatag cggcctccgg gccgggcacc
      781 tcgggcgtgg tcgtgatggc gctgggcggg cccggcttct cggtcgccac cgaggtgggc
      841 cgggcggacg cggtcggtct gcgcctggcc ggggccaagg tcgcggccag cgtgggacgc
      901 atccaggccc gacctcacaa cgtgctgctg ctgctgagcg acgggctggg cggtgaccag
      961 caggaggtgg tgcgcggcgc ccacagcacc ctcggcgccg aggtgcccct cgtcgggggc
     1021 tgcgcgggcg acgacatgaa gatgcgccgg acctaccagt tccacggctc ggaggtgctc
     1081 gaggccgcgg tcgtaggtgc ggccatcggc tccgaaggcc ccatcggcat cggggtggag
     1141 cacggctggc gagcggtggg cgagcccatg gtcgtcaccg gtagctgcga caacagggtc
     1201 gtcagcctgg acgagcagcc cgccctcgac gcctacttcg agcgcctcga cgcccccgag
     1261 gacgtccgcc acgacgccga ggccttcacc cggtacgcca tgacccaccc gctgggcctg
     1321 agccggcgca gcggggacga ggtccgcttc gtggccggcg ccaacttcgc cgaccgctcg
     1381 atcaactgca tcgcggccgt gccccagggc agcctggcgt ggatcatgga gggcgactcc
     1441 gcgtcggtgc tcggagccac cgacgtggca tgtcagcgcg ccctagcagg tctcggcggg
     1501 cgggccccac tgggcctgct cgcattcgac tgcatcgccc gcaagggcgt catgggtgac
     1561 gacggcgtcg ccaccgaggt cgagcgcatc gccggctacg cggggggcgc cccggtcgcc
     1621 ggcttctaca cctatgggga gatcgcccgt acccggggcg tcaacgggtt tcacaaccag
     1681 acgctggttg tggtcgcgtt cgcgtagctc tggtgatggc cccctccggt tgggcgacgc
     1741 agcaactggc cgagttcctg gccgtgctga ccgaagcacc cgacgaccgg gcggccgtgg
     1801 tcgacgcgct cgagcgcatc gccgagtcgt tcgaggccga cgccggcgtc ttcctctccc
     1861 gtcacgaggt cgtgtcatc
//
LOCUS       DASWGG010000017         3126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2920845, whole genome shotgun sequence.
ACCESSION   DASWGG010000017 DASWGG010000000
VERSION     DASWGG010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2920845"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="VF954_00275"
     CDS             complement(<1..162)
                     /locus_tag="VF954_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9529538.1"
                     /translation="MNFSVEGRTAIVTGASSGLGSRFAQVLAGEGANVVLAARRAEPI
                     AQGAKEIEAG"
     gene            327..>3126
                     /gene="recC"
                     /locus_tag="VF954_00280"
     CDS             327..>3126
                     /gene="recC"
                     /locus_tag="VF954_00280"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEX9529539.1"
                     /translation="MLHLHRSERADRLVDALAEVLGDPLEDPMTAEVVAVPTKGVERW
                     ITQRLATHLGTGPGRYDGVCANVAFPFPASLVLGATAAATGIDPELDPWRPERAVWPL
                     LEMVDAALGEPWLAPLAQHLRAITPDDEERPRRFGAVRHLADLFDRYGVHRPEMVQAW
                     AAGAGAALPADTAWQGRLWQLLRGHIGTPSPAERLDDATRALVLDPGLVELPPRLSLF
                     GLTRLPPTYLAVIDALAAGREVHLFLLHPSGALWDKVARILPAPRPGLLRAEDPTAGA
                     AEHPLLASWGRDAREMQIVVTAGSRWSDDHRPVGDRPATLLGHIQAAIRDDHPPERGR
                     GPDGGKGRPFIGPDDRSLQVHSCHGRARQVEVVRDAVMHLLDADPTLEPRDVIIMCPD
                     IETFAPLVHAAFGAAEASDAAGTAGLPDIQVRLADRALRQTNPMLAVAGHLLEMASSR
                     VTATDVLDLAGRDPIRRRFGLDEDDLAQFQSWLAGTGTRWGLDGAHRAHWGLDGLTAN
                     TWEAGLDRLLLGVAMAEEDQRLVGGVLPLDDVGSNSIELAGRLAELIERLSAALRGLS
                     GTHPLTVWAARLAEAVESLAAVAAGGAWQSDQLRGVLDEVGREAGASAAPAVGLSTSE
                     VRALLANRLRGRPTRANFRTGHLTVCTLVPMRSVPHRVVCILGVDDGAFPRSAARDGD
                     DLVLAEPRVGDRDGRSEDRQLLLDALLAATDHLIIAYGGRDVRTNQSRPPAVPIAELL
                     DVAERTVRCRDEAGREIPVRPRLLVEHPLQPFDPRNFEVGRLWPGRSWSFDPIGLEGA
                     KAMASARLAVRTFLPAPLPPRNDSVIELAALVRFVEHPVRAFLRERLGVYVRDPGEGV
                     EDALPVELDNLQKWALGDRLLRAVLAGGDIDACEAAERARGLLPPGALADEVLAEVRG
                     KVAHLAAAVRRRVPPGARP"
BASE COUNT          376 a         1209 c         1154 g          387 t
ORIGIN      
        1 gccggcctcg atctccttgg caccctgggc gatgggctcc gcccggcggg ccgccagcac
       61 cacgttggca ccttcaccgg ccaggacctg ggcgaagcgg gagcccagcc cgctcgacgc
      121 gccggtcacg atggcagtcc gaccctcgac cgaaaagttc atggcgcctt cctcccctgc
      181 cggcggtgcg ctcagccaag caccgcgaac ctaccgcaca tgcgctgggc tgcacagggc
      241 tgcccgttgg caggcggcgt cacacaccct ccgtaaggtg ggagccgtgg gcagagacgg
      301 caaccggtcg agggcgtagg caggcgatgc tgcatctcca ccgctccgag cgagccgacc
      361 ggctggtcga cgccctggcc gaggtgctcg gcgacccgct cgaggacccg atgaccgccg
      421 aggtcgtggc cgtgccgacc aagggcgtcg agcgctggat aacccaacgg ctggcgaccc
      481 acctgggcac cgggccgggc cgctacgacg gcgtgtgcgc caacgtggcg ttccccttcc
      541 cggcgtcgtt ggtgctcgga gcgacggcgg cggcgacggg gatcgacccg gagctcgatc
      601 cgtggcgtcc cgagcgcgcc gtctggccgc tcctcgagat ggtggacgcc gcgctgggag
      661 agccctggct ggcgccgctc gcccagcatc tgcgggcgat cacgcccgac gacgaggaac
      721 ggcctcggcg cttcggggcc gtgcggcacc tggccgatct gttcgaccgg tacggggtgc
      781 accgaccgga gatggtgcag gcgtgggcgg cgggggcggg ggcggccctc ccggccgaca
      841 cggcgtggca gggccggctg tggcagctcc tccgcgggca catcggcacg ccgagcccgg
      901 cggagcgact ggacgatgcc acccgggcgc tggtcctcga ccccggactg gtcgagctgc
      961 cgccgcggct gtccttgttc gggctcaccc ggctgccgcc cacgtacctg gccgtgatcg
     1021 acgcgctggc ggcggggcgg gaggtgcacc tgttcctgct gcatccctcg ggcgcgctgt
     1081 gggacaaggt cgcccggatc cttcctgcgc cccgccccgg cctgctccgc gccgaggacc
     1141 ccacggccgg cgcagccgag catcccctcc tcgcctcgtg gggccgggac gcccgggaga
     1201 tgcagatcgt ggtcacggcc gggtcgcggt ggtcggacga ccaccggccg gtcggggacc
     1261 ggcccgccac gctgctcggg cacatccagg ccgccatccg cgacgaccac cccccggagc
     1321 gcggccgcgg cccggacggg ggcaagggtc gccctttcat cgggcccgac gaccgcagcc
     1381 tccaggtgca cagctgccac ggccgggcgc gccaggtcga ggtcgtccgt gacgccgtca
     1441 tgcacctcct ggatgccgac ccgaccctcg agccccggga cgtgatcatc atgtgccccg
     1501 acatcgagac cttcgccccg ctcgtccacg ccgccttcgg ggcggcagag gcgagcgacg
     1561 ccgccggcac ggccgggctg cccgacatcc aggtgcgcct cgccgaccgg gcgctgcgcc
     1621 agaccaaccc gatgctcgcc gtcgccgggc acctgctcga gatggcctcc agccgggtga
     1681 cggcgaccga cgtcctcgac ctggccggcc gggaccccat tcgccggcgg ttcgggctcg
     1741 acgaggacga cctggcccag ttccagtcgt ggttggccgg caccggcacc cggtggggcc
     1801 tagacggggc ccaccgggcc cactggggac tggacgggct gacggccaac acgtgggagg
     1861 ccggcctgga ccgcctgctg ctgggcgtcg ccatggccga ggaggaccag cgcctggtcg
     1921 ggggggtcct gcccctcgac gacgtgggaa gcaactccat cgagctggcg gggcgcctgg
     1981 ccgagctgat cgagcggctg tccgccgccc tccgcggcct cagcgggacc caccccctca
     2041 ccgtctgggc ggcccgtctg gccgaggcgg tcgagagcct ggccgccgtc gcggccggcg
     2101 gcgcctggca gagcgaccag ctccggggcg tcctggacga ggtcggccgg gaggccgggg
     2161 cgtccgcggc cccggcagtc ggcctcagca cctcggaggt ccgggccctc ctcgccaacc
     2221 ggctgagggg gcggccgacc cgggccaact tccgcaccgg gcacctgacg gtgtgcacgc
     2281 tggtgccgat gcggtccgtc ccgcaccggg tcgtctgcat cctcggcgtc gacgacggcg
     2341 ccttcccccg cagcgcggcg cgcgatgggg acgacctggt cctagccgag ccgcgggtgg
     2401 gcgaccgcga cggccgcagc gaggaccggc agctgctcct ggacgccctt ctcgccgcga
     2461 ccgaccacct gatcatcgcc tacggcggcc gggacgtccg caccaaccag agccgcccgc
     2521 cggcggtgcc gatagccgag ctgctcgacg tggccgaacg cacggtgcgg tgccgggacg
     2581 aggccggccg ggagatcccc gtgcgcccgc ggctgctcgt cgagcacccg ctccagccct
     2641 tcgacccccg caacttcgag gtgggccggc tctggccggg ccgctcgtgg agcttcgacc
     2701 ccatcggcct cgagggcgcc aaggcgatgg cctcggcccg gctggcggtg cgcacgttcc
     2761 tccccgcgcc gctgccgccc cgcaacgact cggtgatcga gctggccgcg ctcgtccgct
     2821 tcgtcgagca ccccgtccgt gccttcctcc gcgagcggct cggggtgtac gtgcgcgacc
     2881 cgggcgaagg cgtcgaggac gccctgccgg tcgagctcga caacctccag aagtgggccc
     2941 tgggagatcg cctccttcgc gccgtgctcg ccgggggcga catcgacgcg tgcgaggcgg
     3001 ccgagcgagc ccgcggcctg ctgccgcccg gcgccctcgc cgacgaggtg ctcgcggagg
     3061 tgcgcggcaa ggtggcccac ctggccgccg cggtgcgccg gcgcgtcccg cctggagccc
     3121 gaccga
//
LOCUS       DASWGG010000018         9851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_987081, whole genome shotgun sequence.
ACCESSION   DASWGG010000018 DASWGG010000000
VERSION     DASWGG010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9851
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987081"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VF954_00285"
     CDS             <1..619
                     /locus_tag="VF954_00285"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX9529540.1"
                     /translation="TAAEAALPGTPPAPPAPPEAIPGRPAAVLVPIFPAAGAAPGAAG
                     RSPGQEAHVLLTRRSTRLRSHTGQVSFPGGRIDEGESAVAAALRESAEEVGLDPGAVE
                     ILGQLAPLSTLSGASFITPFVGLLARRPVLRPNPAEVELAFDVALSHLAAPHVYGAER
                     WVMADGIEHPMHLFALGEDVVWGATARILHQLLELVHHEWTGSLR"
     gene            660..1823
                     /locus_tag="VF954_00290"
     CDS             660..1823
                     /locus_tag="VF954_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEX9529541.1"
                     /translation="MAEVEIGIGKSGRRAYGFDDIAIVPSRRTRDPEDVDISWEIDAF
                     RFDLPLMGAAMDGVISPATAIELGRLGGVGVLNLEGLWTRYEDPEGLFEEIAELDAEK
                     ATARMQQIYSEPIKPELVTRRIQEIKDAGVVSCASLTPQRTEQFAKAILDAELDLLVI
                     QGTVVSAEHVSRTVEPLNLKRFIREFEIPVIVGGCASYQAALHLMRTGAVGILVGVGP
                     GNACTTRGVLGLGVPQATAIADAAGARMRHLDETGVYVQVIADGGMSKGGDIAKAIAC
                     GADAVMIGSPLAAAHEAPGRGYHWGMATFHPTLPRGARVRTEPRGTLKEVVLGPAHEN
                     DGRLNLFGALRSSMATCGYESVKEFQKAEVMVAPALQTEGKQLQRAQGVGMGH"
     gene            1991..3139
                     /locus_tag="VF954_00295"
     CDS             1991..3139
                     /locus_tag="VF954_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9529542.1"
                     /translation="MSSSAQQLDGSHPARRVADLLPIGVVVADLEGSAIFANHAWTAM
                     AGHAGDGWRGAGWLGVLDAGDRDQARRALLAAISSGAAHESDWVVTDSTQEARVFHVT
                     AVPDFGDGGPVGLVAAIRDVTEERRARDGLAPTPPQPPPGAAGRLQTAATNDALDLLA
                     AAAHELRTPLSAIAGYAATLRENREIMSSEDIDRSFMALERQTGRLTTLLDNLLDLGR
                     WRHGAGAEDGPGSAPVRVADAFGDALEAAPPPDGVIVTVARSSPSLHVRAGRSGLTQV
                     LVNLLTNAYRYGGPHITLDSHEEASTVVLDVVDDGAGVPLDLQPKMFLPFCGGGNGSR
                     PRPGAAGLGLALATRIVEFSGGRLSYQAAQPHGAHLIVTLPRAEDDPR"
     gene            complement(3150..3860)
                     /locus_tag="VF954_00300"
     CDS             complement(3150..3860)
                     /locus_tag="VF954_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529543.1"
                     /translation="MSFTTIGSRPLGQVGRLATVAVLSAAALTGCGHGSDTSATASDR
                     SSSDNGGRSGADQNSSTPSTVAVARGPVILRQTPPPWGTPGPGEEAKAISAAGLSFLH
                     QPGQVQHTHTHLDISIDGQPVAVPAEIGFDLGVQVTAPILTRDGSGIVHVESPTARTF
                     TLGELFTEWEVRLTSACIGVTCTSASEVVRFYVNGYAFAGDPNSIALRSHQEIAIVAA
                     AAGATAAPPSSYNFPGGY"
     gene            3962..6172
                     /gene="pcrA"
                     /locus_tag="VF954_00305"
     CDS             3962..6172
                     /gene="pcrA"
                     /locus_tag="VF954_00305"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEX9529544.1"
                     /translation="MPASDTSLLDGLNPVQREAVTHGDGPLLIIAGAGSGKTRVLTHR
                     VAWLVREQGVSPFQVLAITFTNKAADEMKHRVAALVGPVAERMWVSTFHSACVRILRR
                     DAGRLGYRSSFTIYDEADAVRLTGYVLRDLNIDAKRFPPRTVHGVISQAKNELVDFET
                     FRAHARTLYDRKIADVYAEYQRRLLAASAMDFDDLLMVTVNLLQSQPEVLAHYQERFR
                     HILVDEYQDTNRAQNELILLLGQAHQNVCIVGDSDQSVYRFRGADIRNILEFENAFPN
                     ARTIVLEQNYRSTQTILDAANAVIANNAMRKPKALWTEQVGGELLTRYFAEDEGDEAR
                     WLVGELERLHSSEHYRWADVAVFYRTNAQSRVVEEELVRHDIPYKIVGGTRFYDRREV
                     KDLLAYLKAVANPADEVSLKRIVNVPKRGVGDTSIARLDAWATTRGVPFAEALAEAEA
                     AGVAGKALSGIRDLRAFLDRLRGLVAHEQAGPARLLEEVSDRTGYRAELEAERSIEAA
                     GRLENIAELIGTAMDFGSLDEFLEAVSLVADADEIDADESKALLMTLHTAKGLEFPVV
                     FVIGLEDGVFPHLRSLGDPDELEEERRLCYVGITRARERLYLTNAWCRTLWGSTQYNP
                     PSRFLKEIPGELTREVASVRMRERASRGPRPGDARDAIVESAMRTRVAASPARTTGAE
                     SLGLKAGDDVMHAKWGEGVVLEVIGGDDKAEAIIRFPSVGEKRLLLAWAPVKRA"
     gene            complement(6192..7799)
                     /locus_tag="VF954_00310"
     CDS             complement(6192..7799)
                     /locus_tag="VF954_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9529545.1"
                     /translation="MRLADYLGLAWRSLRRSRLRSGLTVSAIVVGATGLTIMLTFVTS
                     VKSFVTAQFVSTGQIRQITVGQNVNLSYSPAGEVGGPAPLAPAADAPAGTGAQGGAPG
                     PAAGTITLGPDMEAKITAIPHVTGVSAVLHSGNAIEYLSLGSKQLQVFGLNGYEPNGV
                     IKPVLLAGRDLRPSDASGTLLVSQDYADALGFAGNYARLVGQTVDLHTMRGYSGAGAV
                     LPDLLPPGGSNDCQGQRNCIGGPMASLPSLAIPATVAGVVDRSFEGQAVFVPLSWLVA
                     LDNQARPEMRPDQQPANGFGGSPQSPQPGRAGQPGQGPGPGQGQGQGPGQAQGQQGPG
                     TSHVTWTHLDVASYIAGRGGYDSFLVAADNVGNVAAVAASIDRLGVKTATGLKQLNDQ
                     KSKANIVGAILGSLGLVALGIAALGVMNTMVMSVLERTREIGVMRALGARRSTIRRLF
                     TFEAASLGFLGGLFGVVIGEAVVLGAKPVIRSVVKSGKVSGVSFSVPLWLILVVLGIT
                     TSIGLMSGLLPARRAARLDPVEALRYE"
     gene            complement(7799..8515)
                     /locus_tag="VF954_00315"
     CDS             complement(7799..8515)
                     /locus_tag="VF954_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9529546.1"
                     /translation="MGRPNSTNSGGSSPAVQLTDVTKTYQLGEERFHALDHVELAIAE
                     GDFVAIMGPSGSGKSTLANIIGGLDRLDAGEVLVAGENLARMGDGALSRFRNEKVGFI
                     FQAFNLKADGTALENVMLPMVFARVRPAERKARAAECLGRVGLAARQDRLPSQLSGGE
                     RQRVAIARAMAMRPALIIADEPTGNLDSARGKEVIDMLAELNADGMTLIVITHDPSIG
                     ARAHRIVEVLDGRLREPART"
     gene            complement(8631..>9851)
                     /locus_tag="VF954_00320"
     CDS             complement(8631..>9851)
                     /locus_tag="VF954_00320"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX9529547.1"
                     /translation="LPMVFPGPAPARMVVAARSGSTRRLQQRLDRARAHGVSDQPTIA
                     AAQILALVGALHAHDRRTRGHAERVRTLTELVATELRLDADSRDRLRWASLLHDIGKL
                     EVPRRILNKPGSLNAKEWVAIHRHPADGARIAAPLAAWLGPWSRAIAEHHERYDGTGY
                     PLGLSGDDISEGARIVAVADAFEVMTAARAYKRAMSPALACQELATQAGTQFDERVVA
                     AFLAISFTRLRWLTGPVAWVAQLPILGWLPRVLAGTGALGTQAAGIAGVAALAGASTV
                     ATPALASAGSVIPLGPPVPPAASAPLGPAGAVHRGSAESRVAPTANHDRSPAPGGPSA
                     SAATTASTAPGHGSANGSSGNGSSANSTGAVATFTKGNANNAHPAGVPAVSTSSTLAP
                     DAPKAPGSGHTRIG"
BASE COUNT         1385 a         3521 c         3594 g         1351 t
ORIGIN      
        1 cacggcagcc gaggcggccc tgcccggcac cccgccggcc ccgccggccc cgccggaggc
       61 catccccggc cggccggcgg ccgtgctcgt ccccatcttc ccggcggcgg gagccgcccc
      121 cggtgccgcc ggccgctctc ccgggcagga ggcccatgtc ctgttgaccc gccgctccac
      181 caggctccgc tcccacacgg gccaggtctc gttccccggc ggtcggatcg acgagggcga
      241 gtccgccgtc gccgcggccc tgcgcgagtc ggccgaggag gtcggcctcg accccggcgc
      301 ggtggagatc ctggggcagc tggcgccgct cagcaccctg tccggcgcca gcttcatcac
      361 cccgttcgtg gggctgctgg cgcggcgccc ggtcctgcgg cccaatccgg ccgaggtcga
      421 gctggccttc gacgtcgccc tctcccatct ggctgcgccc cacgtgtacg gggccgagcg
      481 atgggtcatg gccgacggca tcgagcaccc gatgcacctg tttgccctcg gcgaggacgt
      541 ggtctggggc gccaccgctc gcatcctgca ccagctcctg gagctggtcc accacgagtg
      601 gacgggctcg ctccggtaga ctcggctgtt ccgggctgag gtgcgacggg aggagagccg
      661 tggctgaggt cgagatcggc attggcaagt cgggccggcg ggcgtacggg ttcgatgaca
      721 tcgccatcgt cccgagccgg cgcacgcgcg accccgagga cgtcgacatc tcgtgggaga
      781 tcgacgcctt ccgcttcgac ctcccgctga tgggcgcggc catggacggt gtgatcagcc
      841 cggcgaccgc catcgagctc gggcgcctcg gcggggtcgg cgtcctcaac ctggaggggc
      901 tctggacgcg ctacgaggac ccggagggcc tcttcgagga gatagctgag ctcgacgcgg
      961 agaaggccac ggcccgcatg cagcagatct actccgagcc catcaagccg gagctggtca
     1021 cccgccgcat ccaggagatc aaggacgccg gcgtggtgtc gtgcgcgtcg ctcacgcccc
     1081 agcgtaccga gcagttcgcc aaggccatcc tggacgccga gctcgacctg ctggtcatcc
     1141 agggcaccgt ggtgtcggcc gagcacgtgt cccgcaccgt cgagcccctc aacctcaagc
     1201 ggttcatccg ggagttcgag atccccgtca tcgtgggcgg gtgcgcctcg taccaggccg
     1261 cgctgcacct gatgcggacc ggagcggtcg gcatcctggt cggagtcggc cccggcaacg
     1321 cctgcaccac gcgcggggtg ctcggcctgg gcgttcccca ggcgaccgcc atcgccgacg
     1381 cggccggcgc ccgcatgcgc cacctcgacg agacgggcgt ctacgtacag gtgatcgccg
     1441 atggcggcat gagcaaaggc ggcgacatcg ccaaggccat cgcgtgcggg gccgacgccg
     1501 tgatgatcgg ctcgccgctc gccgccgccc acgaggcgcc gggccggggc taccactggg
     1561 ggatggcgac gttccatccc accctgccgc gcggcgcccg cgtgcgcacc gagcccaggg
     1621 ggacgctcaa ggaggtcgtg ctcggccccg cccacgagaa cgacggccgg ctcaacctgt
     1681 tcggcgcgct ccgctcgtcg atggccacgt gcggctacga gtcggtgaag gagttccaga
     1741 aggccgaggt catggtggct cccgccctcc agaccgaggg caagcagctc cagcgggccc
     1801 aaggcgtggg gatgggccac tagcaacggc cgtggcctcg gctggcccgg gggcggttcg
     1861 cgaggtttgc cccccttccg cgggacccgg gcggcgagta cgatgatcac tatcggcgtc
     1921 ggttctcggt gccgacgcga gccccagacc ccggtcgctg catgagtcgc ctgcactgac
     1981 ggaggtcagg gtgtcgagtt cagcgcagca actagacggt agccacccgg cgcggcgggt
     2041 cgcggatctg ctgccgatcg gagtggtcgt cgccgacctg gaaggctcgg ccatcttcgc
     2101 caaccacgcc tggacggcca tggcgggcca tgccggcgat ggctggcgtg gggcgggctg
     2161 gctcggcgtg ctggacgccg gcgaccgcga tcaggcgcgc cgcgccctcc tggccgccat
     2221 ctcgtcggga gccgcccacg agtcggactg ggtggtcacc gactcgaccc aggaggcgcg
     2281 ggtgttccac gtcaccgccg tacccgactt cggcgatggt gggccggtcg ggctggtggc
     2341 cgccatcagg gacgtcaccg aggagcggcg cgctcgcgac gggttggcgc cgaccccacc
     2401 gcaacctcca ccgggcgccg ccggaaggct gcagacggcg gccaccaacg acgcgctcga
     2461 tctcctcgcc gccgccgccc atgagctgcg gaccccgctc tcggccatcg cgggctacgc
     2521 cgccaccctg cgcgagaacc gggagatcat gtcgtcggag gacatcgacc ggtcgttcat
     2581 ggccttggag cgccagaccg gccggctgac gacgcttctc gacaacctgc tcgacctggg
     2641 ccgctggcgc cacggcgctg gggcggaaga tgggcccggc agcgctcccg tccgcgtggc
     2701 cgacgccttc ggcgacgccc tcgaggccgc gccgccaccc gatggcgtga tcgtgaccgt
     2761 cgcccgttcg tctccgagcc tgcatgtccg cgccggccgg tcggggctca cccaggtgct
     2821 cgtcaacctg ctcaccaacg cctaccggta cggcgggccc cacatcacgc tcgactccca
     2881 tgaggaggcg agcaccgtgg tcctcgacgt ggtggacgac ggggcggggg tccccctcga
     2941 cctccagccg aagatgttcc tacctttctg cgggggcgga aacgggagtc ggccccgacc
     3001 cggcgcagcc gggttggggc tggccctcgc cacccgtatc gtcgagttct cgggcggccg
     3061 gctctcctac caggcggccc aaccccatgg cgcccacctc atagtcaccc tcccccgcgc
     3121 cgaggacgac ccccggtagg ggcgacggct caatacccgc cggggaagtt gtagctcgaa
     3181 ggcggggcgg cggtggcacc cgcggctgcg gccacgatgg cgatctcctg gtgtgaccgc
     3241 aacgcgatgc tgttggggtc ccccgcgaac gcgtatccgt tcacgtagaa cctgaccacc
     3301 tccgacgcgc tggtgcaggt caccccgatg cacgccgaag tcaggcgcac ctcccactcg
     3361 gtgaacagct cgcccaacgt gaaggtccgc gccgtcggcg actcgacgtg gacgatgccg
     3421 ctgccgtccc gggtcaggat gggcgcggtg acctgcacgc cgagatcgaa gccgatctcc
     3481 gccggcacgg ccacgggctg gccgtcgatc gagatgtcca ggtgggtgtg ggtgtgctgc
     3541 acctgcccgg gctgatggag aaaggagagc cctgcggcgg agatggcctt ggcctcctca
     3601 cccgggccgg gcgtccccca gggcggaggg gtctggcgca ggatgacggg ccccctggcc
     3661 acggcgacgg tcgacggggt cgaggagttc tggtcggcgc cggaccggcc gccgttgtcc
     3721 gacgacgacc ggtcggacgc ggtggcgctg gtgtcgctgc cgtggccgca gcccgtcaaa
     3781 gcggccgcgc tcagcacggc gacggttgcc aatcggccca cctgccccag ggggcgcgac
     3841 ccgatggtgg tgaacgacac ggctcgacca cgctaccgac gaggccgggc gaggtcatgt
     3901 cgcctcacgg cggggcgctc ggccgctgag ggtgcacagg gcgccggtag cctcgctagg
     3961 gatgcccgcc tccgacacct cgctgctcga cgggctgaac ccggtgcagc gggaggcggt
     4021 gacccacggc gacgggccgc tgctgatcat cgccggggcg ggatcgggca agacccgcgt
     4081 gctcacccac cgggtggcct ggctcgtccg cgagcagggc gtgtcgccgt tccaggtcct
     4141 cgccatcacc ttcaccaaca aggccgccga cgagatgaag caccgggtcg cggccctggt
     4201 ggggccggtg gccgagcgga tgtgggtgtc gacctttcat tctgcctgcg tgcgcatcct
     4261 gaggagggac gcggggcgac tcggctaccg gtcctctttc accatctacg acgaggccga
     4321 cgccgtgcgc ctcaccggct acgtgctgcg cgacctcaac atcgacgcca agcgcttccc
     4381 gccccgcacc gtccacggcg tgatcagcca ggccaagaac gagctggtcg acttcgagac
     4441 cttccgcgcc cacgcccgga cgctgtatga ccgcaagatc gccgacgtct acgccgagta
     4501 ccagcgccgg ctgctggccg ccagcgccat ggacttcgac gacctcctca tggtcaccgt
     4561 caacctcctc cagtcccagc ccgaggtgct ggcccactac caggagcggt tccgccacat
     4621 cctggtggac gagtaccagg acaccaaccg ggctcagaac gagctgatcc tgctgctcgg
     4681 ccaggcccac cagaacgtct gcatcgtggg cgacagcgac caatcggtgt accgatttcg
     4741 cggcgcggac atccgcaaca tcctggagtt cgagaacgcc ttccccaacg ccaggaccat
     4801 cgtcctggag cagaactacc ggtccaccca gacgatcctg gacgcggcca acgcggtcat
     4861 tgccaacaac gccatgcgca agcccaaggc gctgtggacc gagcaggtgg gcggcgagct
     4921 gctgacccgg tacttcgccg aggacgaggg cgacgaggcc cggtggctgg tgggcgagct
     4981 ggagcggctc cactcctccg agcactatcg gtgggccgac gtggccgtct tctaccgcac
     5041 caacgcccag agccgggtcg tcgaggagga gctggtccgc cacgacatcc cgtacaagat
     5101 cgtgggcggc acccgcttct acgaccggcg ggaggtcaag gacctgctgg cctatctcaa
     5161 ggcggtggcc aacccggccg acgaggtctc gctcaagcgc atcgtcaacg tccccaagcg
     5221 cggcgtgggg gacacctcca tcgcccggct ggacgcgtgg gcgaccaccc ggggcgtgcc
     5281 gttcgccgag gcgctggcgg aggcggaggc ggccggggtc gccggcaagg cgctgtcggg
     5341 gatcagggac ctgcgggcgt tcctcgaccg gctgcgcggc ctggtcgccc acgagcaggc
     5401 cggcccggcc cggctgctcg aggaggtcag cgaccgcacg ggttaccggg ccgagctgga
     5461 ggccgagcga tcgatcgagg ccgccggacg gctggagaac atcgccgagc tcatcggcac
     5521 ggcgatggac ttcgggtcgc tggacgagtt cctcgaggcg gtgagcctgg tggccgacgc
     5581 cgacgagatc gacgccgacg agtccaaggc gctgctcatg accctgcaca cggccaaggg
     5641 cctggagttc ccggtggtgt tcgtgatcgg gctcgaggac ggcgtgttcc cccacctccg
     5701 ttcgctgggc gatcccgacg agctggagga ggagcgccgg ctctgctacg tgggcatcac
     5761 ccgggccagg gagcggctgt acctgaccaa cgcctggtgc cgcaccctgt ggggctcgac
     5821 gcagtacaac ccgcccagcc gcttcctgaa ggagatcccc ggcgagctga cccgtgaggt
     5881 ggccagcgtt cgcatgcggg agcgggcctc gagaggaccc cgccccggcg acgcccgcga
     5941 cgccatcgtc gagtcggcca tgcgcacccg cgtggccgcc tcaccggccc gcacgaccgg
     6001 cgccgagtcg ctcgggctca aggcgggcga cgacgtgatg cacgccaagt ggggcgaggg
     6061 cgtcgtgctc gaggtgatcg gcggggacga caaggccgag gccatcatcc gcttcccgag
     6121 cgtcggggaa aagcgcctct tgttggcctg ggcccccgtg aagcgggcct gacctcgatg
     6181 ggcaccgggg cctactcgta gcgaagggct tcgaccgggt ccagccgcgc cgcccggcgg
     6241 gcgggcagga gccccgacat gaggccgatc gaggtggtga tgccgaggac caccaggatc
     6301 agccacagcg gcaccgagaa gctgactccg gacaccttcc cggacttgac cacgctgcgg
     6361 atcaccggct tggccccgag cacgacggcc tcgccgatga ccaccccgaa caagccgccc
     6421 aggaacccga gggaagccgc ctcgaacgtg aacaacctcc tgatggtcga gcgccgggcg
     6481 cccagggcgc gcatcacgcc gatctcgcgg gtgcgctcca gcacggacat caccatggtg
     6541 ttcatcacgc ccagggcggc gatgcccagg gcgaccaggc cgaggctgcc caggatggcg
     6601 cccacgatgt tggccttgga cttctggtcg ttgagctgct tcaggccggt ggcggtcttg
     6661 accccgagac gatcgatgct cgccgccacg gcggccacgt tgccgacgtt gtcggccgcg
     6721 acgagaaacg agtcgtagcc gccgcgaccg gcgatgtagc tcgccacgtc cagatgggtc
     6781 caggtgacgt gggacgtccc cggtccctgc tgcccctggg cctgccccgg gccctgcccc
     6841 tggccctgac ccggccccgg gccctgcccg ggttggcccg ccctgccggg ctggggcgac
     6901 tgcggcgagc ccccgaagcc attggccggc tgctggtcgg gccgcatctc cggccgggcc
     6961 tggttgtcga gcgcgaccag ccacgagagc gggacgaaaa cggcctgccc ctcgaatgag
     7021 cggtccacca cgcccgccac cgtggctggg atggcgaggg acgggaggct ggccatcggg
     7081 cccccgatgc agttgcgctg gccctggcag tcgttcgaac cgccgggtgg gagcaggtcc
     7141 ggcaggaccg cccccgcgcc gctgtagccc ctcatggtgt gcaggtcgac cgtctggccg
     7201 acgagccggg cgtagttgcc cgcgaacccg agggcgtcgg cgtagtcctg cgagacgagc
     7261 agggtgcccg acgcatcgga cgggcggagg tcccggccgg cgagcaggac cggcttgatc
     7321 acaccgttcg gctcgtagcc gttcaggccg aacacctgga gctgcttgct gcccaggctg
     7381 aggtactcga tggcgttgcc gctgtggagc acggcggaga cgccggtcac gtgggggatg
     7441 gcggtgatct tcgcctccat gtcgggcccg agggtgatcg tgcccgccgc cggtccgggt
     7501 gcgccgccct gtgcgccggt gccggcgggc gcgtcggccg cgggcgcgag gggcgccggg
     7561 ccgccgacct cgccggccgg gctgtaggag aggttcacgt tctggcccac ggtgatctgg
     7621 cggatctggc cggtggagac gaactgggcg gtgacgaagc tcttcacgct ggtcacgaag
     7681 gtcagcatga tcgtcagccc ggtggccccc acgacgatgg cggagacggt caggccggag
     7741 cgcagccggg agcgacgcag gctccgccac gccagcccca ggtaatcggc caggcgcatc
     7801 aggttctcgc cggttcccgc aggcgaccgt cgaggacctc gacgatccgg tgggcccggg
     7861 cgccgatcga cgggtcgtgg gtgatcacga tcagggtcat cccatcggcg ttgagctcgg
     7921 cgagcatgtc gatgacctcc ttgccccggg cgctgtcgag gttgccggtc ggctcgtcgg
     7981 cgatgatcag cgccggacgc atggccatgg cccgggcgat ggcgacccgc tggcgctcgc
     8041 cgcccgacag ctggctcggg aggcggtcct gtcgcgccgc caggcctacg cggccgaggc
     8101 actcggcggc gcgggccttg cgctcggccg gccggacgcg ggcaaagacc atgggcagca
     8161 tcacgttctc gagggccgtg ccgtcggcct tcaggttgaa ggcctggaat atgaagccca
     8221 ccttctcgtt gcggaaccgc gacagcgccc cgtcgcccat gcgggccagg ttctcccccg
     8281 cgaccaggac ctcgcccgcg tcgaggcgat cgagcccgcc gatgatgttg gccagcgtgg
     8341 acttgcccga ccccgacggg cccatgatgg cgacgaagtc cccctcggcg atggcaagct
     8401 cgacgtggtc tagcgcgtgg aagcgctcct cgcccagctg gtaggtcttg gtgacgtcgg
     8461 tcagctgcac ggccggggac gagcccccgg aattggtcga gttcggtcgc cccatcgagg
     8521 ccaacggtac gaatcggcgc gaggggagtc catggccgat ttcacaacca ccccaatgag
     8581 ccaccgagtc catggaccgg tgccagcttc atggtgggat ggctgaggcg tcaaccgatc
     8641 cgggtgtggc ccgacccggg ggccttgggg gcgtccggcg cgagcgtgga ggacgtggag
     8701 acggcgggca ccccggcggg gtgggcgttg ttggcgttgc ccttggtaaa ggtggccacg
     8761 gccccagtgc tgttggccga gctgccgttg cccgagctgc cgttggccga gccgtggccc
     8821 ggcgccgtgg atgcggtggt cgcagccgat gccgagggcc cgccgggcgc cgggctccgg
     8881 tcgtggttgg cggtaggcgc cacccgggac tcggcggagc cccggtgcac cgcccccgcc
     8941 gggcccagcg gggccgaggc cgcgggcggc accggggggc cgagggggat caccgaaccc
     9001 gcgctggcga gggcgggagt ggccaccgtg ctggcgccgg ccagagccgc cacgcccgcg
     9061 atccccgccg cctgggttcc gagggccccg gtcccggcga gcaccctcgg gagccatccg
     9121 aggatgggta gctgggcgac ccacgccacc gggccggtga gccagcgcag ccgggtgaag
     9181 gagatggcga ggaaagccgc caccacgcgc tcgtcgaact gcgtgccggc ttgggtcgcc
     9241 agctcctggc acgccagagc cgggctcatg gcgcgcttgt aggcgcgggc ggccgtcatg
     9301 acctcgaagg cgtcggccac ggccacgatg cgagccccct ccgagatgtc gtcgccactg
     9361 agcccgagcg ggtagccggt cccgtcgtag cgctcgtggt gctccgcgat ggcccgcgac
     9421 cacggcccga gccaagccgc caggggagcc gcgatccggg cgccgtccgc cgggtggcgg
     9481 tggatggcga cccactcctt ggcattgagc gagcccggtt tgttgaggat ccgcctcggg
     9541 acctccagct tgccgatgtc gtggagcagg ctggcccacc gcagccggtc gcggctgtcg
     9601 gcgtcgagcc tgagctcggt ggcgaccagc tcggtcaggg tccggacccg ctcggcgtgg
     9661 ccccgggtcc gccggtcgtg ggcgtggagg gcgccgacca gagcgaggat ctgggccgcg
     9721 gcgatggtcg gctggtccga caccccgtgg gcccgggcgc ggtcgaggcg ctgctgcagc
     9781 cggcgggtgc tgcccgagcg ggcggccacg accatccggg ccggggccgg gccggggaac
     9841 accatcggga g
//
LOCUS       DASWGG010000019         3028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_332463, whole genome shotgun sequence.
ACCESSION   DASWGG010000019 DASWGG010000000
VERSION     DASWGG010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332463"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..806
                     /locus_tag="VF954_00325"
     CDS             144..806
                     /locus_tag="VF954_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529548.1"
                     /translation="MPTNDLSSRDRIVEYLRSRGGSISNDEGRGLTQALALAAGYQDL
                     SVLNGMLSRLEREGVIERDVRGRRTYGISLGGAEGAPEREAVAAPRGGRTGGGGRGGR
                     REGRRGGRSATAPSRRPAMSANGGAAGVASGIASGVGVPTAPAAPPDEERWDEMEATV
                     RRLRSEVAQLTARLERVEAGASDVAGLASQVDELNRHADSQRAAAAAGEAPARRGLFG
                     RR"
     gene            1006..2184
                     /locus_tag="VF954_00330"
     CDS             1006..2184
                     /locus_tag="VF954_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529549.1"
                     /translation="MGALAVALLTGLAVVPGCGHAGARSGSSATASHAVAADAAAAAG
                     AGRAVPSSITPGPQPATFTPPVPAPTAPASAAVAASQQPASPPALPLGGCPPPPYTPP
                     SNPAKPWRPPVLVPEASLPSAPPPTPLTADTSALDGKGMWIWQFRRTEGGDPAAVVRR
                     AAVAGLSQLWVRVGDSRDGFYAASYLSELVPLSHAAGLAVIGWGFPYLWDPVGDAAWT
                     AQAYGWRAAGGDMLDGFSADIEAPTEGTALSMRRVAVYLGLVRAGAPGRLLVATVYRP
                     NDYRWSTYPYATMAPYVDAFAPMVYWGCDQPGPETLQAVSRLSTLRPVHVIGQAYNMA
                     AEGGRTQAPGPAELHQVLDLAWSGGAAGVSFWDWQEMTGEEWAELARYSWFRPAPHVG
                     "
     gene            2388..>3028
                     /locus_tag="VF954_00335"
     CDS             2388..>3028
                     /locus_tag="VF954_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529550.1"
                     /translation="MARRLVFGRAGKRATRALLGAALVGAGTLSLIGGTVTPAGAAPG
                     DLDASFGSGGIVVDGASKSIQANSVVLQPDGSIVLAGSAANKANSFESFSIARLTPGG
                     AADGSFNGGNPAQPSFGSQTNDVANGVVPYPGGRFLAVGSTTASPDGNQTVIAVARLN
                     NDGSTDGSFGSGLHTTWASAGCVSTPCAISPTPGTVALQVVPSSNSHDAGFGAV"
BASE COUNT          397 a         1067 c         1142 g          422 t
ORIGIN      
        1 tccccccaac ctacgcgctg ttcacgcgaa aggtcatgcc acaggatggt gtgagggtgt
       61 cgtcctcggt ggcatcgaca tacaatccga cattcgggct ttttcggccc acccacgccc
      121 ccgaagactc ggaggaagag ctggtgccca ccaacgacct gtccagccgt gaccggattg
      181 tcgagtacct ccgctcccgg ggcggctcca tctcgaacga cgaaggccgg gggctgaccc
      241 aggccctggc cttggcggcc ggataccagg atctcagcgt cctcaacggg atgctgtccc
      301 ggctcgagcg ggagggcgtc atcgagcgcg acgtccgcgg ccggcgcacc tacggcatct
      361 cgctcggcgg ggccgagggt gctccggagc gggaggcggt cgcagcccct cgtggggggc
      421 gaaccggggg tggagggcgc gggggccggc gcgagggccg gcgtgggggg cgatccgcca
      481 ccgccccgag ccgccggccg gctatgagcg ccaacggcgg ggctgccggc gttgctagcg
      541 gcattgctag cggcgtgggc gttccgacgg ccccggcggc cccgcctgac gaggagcggt
      601 gggacgagat ggaggcgacc gtgcgccgcc tgcgctccga ggtggcccag ctcacggctc
      661 gactcgagcg ggtcgaggcg ggggcgtcag atgtcgccgg cctggcctcg caggtggacg
      721 agctgaaccg gcacgccgat tcccagcgcg ccgccgcggc agccggggag gcgcccgccc
      781 ggcgggggct gttcggccgc cgctgacacg gcgggaccag ttagctgggg agatttggtg
      841 gtctgagacc acctcgtttc cccagcaacg acgtggctgg cgcgggcgcg tgccagccag
      901 ctggtctgcc agacgcggta aagtgagcgg ccaacggggg agagcggact ccgggggggt
      961 ttgcgcgtgg gaccggccgg acgacgaagc gccaccaggc ggccggtggg cgcccttgcg
     1021 gtcgcccttc tgaccgggct ggccgtcgtg cccggctgtg gccatgccgg ggcccgctcc
     1081 gggtcgtccg cgacagcctc gcacgcagtc gccgcggacg ccgccgcggc ggccggcgcc
     1141 ggccgcgcgg tcccgtcgtc catcaccccg ggcccgcagc cggcgacgtt caccccacct
     1201 gtgccggcgc ccacggcgcc ggcttcggcc gcggtcgccg cgtcgcaaca gccagcgtcc
     1261 ccgccggcgc tgccgctcgg gggctgcccg cccccgccgt acacccctcc ctcgaacccg
     1321 gccaagccct ggcgaccgcc agtcctggtg cccgaggcat cgttgccctc agcccctccg
     1381 cccaccccgc tgaccgccga cacctctgcc ctggacggca aggggatgtg gatctggcag
     1441 ttccggcgaa cggagggcgg agacccggcg gccgtggtgc ggcgagccgc ggtggcgggg
     1501 ctcagccagc tttgggtgcg ggtcggggac tcccgcgacg ggttctatgc ggcctcctac
     1561 ctctccgagc tggtgccgct gtcccatgcc gccggcctgg cggtcatcgg gtggggcttt
     1621 ccctacctgt gggacccggt cggggacgcg gcctggaccg cgcaggccta cggctggcgg
     1681 gctgcgggcg gcgacatgct cgacgggttc agtgcggaca tcgaggcgcc caccgagggc
     1741 accgcgctga gcatgcgccg ggtcgcggtc tatctgggcc tggtgcgggc gggcgcgccc
     1801 ggacggctcc tggtcgccac cgtctaccgg cccaacgact accggtggtc cacctatccc
     1861 tacgccacca tggccccgta cgtcgacgcc ttcgccccga tggtgtactg ggggtgcgac
     1921 cagcccggcc cggagacgct ccaggccgtg agccgcctgt cgacccttcg ccccgtgcac
     1981 gtcatcggcc aggcctacaa catggcggcg gagggcgggc gcactcaggc gccgggcccc
     2041 gccgagctcc accaggttct cgacctggcc tggtcgggcg gcgcggccgg cgtctcgttc
     2101 tgggactggc aggagatgac aggcgaggaa tgggccgagc tggcccgcta ctcgtggttc
     2161 cggccggcgc cgcacgtcgg gtaaagccct caccctctca ttgaggttga ggttggcaag
     2221 gtgagggaga cgggtggatg ccctttgggg cagtctcgcg ccgaatcggt cgacaccggc
     2281 gcggaccggg ctattctttg gctctgaact cgggggcaac gggtgtcccg aggggggccg
     2341 ggaggcacag agagttgagc ggcggggggc aggaacgggc aaggactatg gcgaggcgcc
     2401 tcgttttcgg gcgcgccggg aagcgggcga cacgagccct tctgggcgcc gccctggtcg
     2461 gtgccggcac gctcagcctc atcggcggca ccgtgacacc tgccggcgcg gctccgggcg
     2521 acctcgacgc cagcttcggg tccgggggga tcgtggtgga cggggcgagc aagtcgatac
     2581 aggccaactc ggtggtgctg caaccggacg gctcgatcgt gctcgccggc tcggcggcca
     2641 acaaggcgaa ctcgttcgag agcttctcga tcgccagatt gactccgggc ggcgcggccg
     2701 acggctcgtt caacggagga aaccccgcgc agcccagctt cggcagccag accaacgacg
     2761 tcgccaacgg ggtggtgccc tacccgggtg gcaggttcct cgcggtcggc tcgacgacgg
     2821 caagcccgga tggcaaccag acggtcatcg cggtggcgcg gctcaacaac gacggctcca
     2881 ctgatgggag cttcgggtcg ggccttcaca ccacgtgggc gagcgcagga tgcgtctcga
     2941 ccccgtgtgc catcagcccg acgcccggaa cggtcgcgct ccaggtggtg ccgtcgagca
     3001 attcccacga tgccggtttc ggtgcggt
//
LOCUS       DASWGG010000020         4330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1944374, whole genome shotgun sequence.
ACCESSION   DASWGG010000020 DASWGG010000000
VERSION     DASWGG010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944374"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..756)
                     /locus_tag="VF954_00340"
     CDS             complement(31..756)
                     /locus_tag="VF954_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9529551.1"
                     /translation="MSAPDVQVLRWGQERARIGPWRGQDRVAYLAPVADAPVPSAAFV
                     RRCMDQLSARGFSQVVTGALAPAEQDAFLSAGFRVTERLHLLGRNLEQLPAAPRPPGV
                     AFRRARASDRHDVLEVDRVAFPPFWQLDDAGLEEALEATPRARYRVATPGTGAIAGYA
                     ITGRAARHGFLQRVAVHPGHQGRGLGAALIVDGLRWLRRWGAERVVVNTQRENAAALA
                     LYERLGFAHEPFGLSVLSAGLLP"
     gene            850..1929
                     /locus_tag="VF954_00345"
     CDS             850..1929
                     /locus_tag="VF954_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX9529552.1"
                     /translation="MLDLTELLRYTVEQGGSDLHVKVGSVPHVRVDGHLMPAPFDRVA
                     AAEAERMGFTILPKERADEFVETAEADFAHSVPGLGRFRVNIFRQRGSVGLVFRRVLP
                     GIPSFESLGLPGVVRRLADENRGLVLVTGPTGSGKTTTIAAMIDHINATKAVNIVTIE
                     DPIEVLHVDNKALVNQREIGSDTRDYSMAMKRVLRQDPDVIFVGEMRDPETVAAALAA
                     GETGHLVFSTLHTTDAVETVNRIVDFFPPYQQRQVRLSLASSLRGVISQRLLERADRR
                     GRVPAVEVLVMTGRVFDRVIDSDTQETIEEVIADGEYYGMQTFDQSLFGLYKDGLVSL
                     RDAMAASTHPHDLRIKLQQAGVLAV"
     gene            1973..3355
                     /locus_tag="VF954_00350"
     CDS             1973..3355
                     /locus_tag="VF954_00350"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX9529553.1"
                     /translation="MIPDRFRPLVELTSSLAARFQAAGFSLYLVGGVVRDHILGVAAE
                     GSDLDFTTDARPDDVEGLLSGWAESVWLQGKRFGTVGAWRGGLRHEITTHRAEAYAAD
                     SRKPEVAFSDDVAVDLSRRDFTVNAMALRLPDLTLVDPFDGVADLAAGRLRTPLSPEE
                     SFNDDPLRMLRAARFMAGFGLKPDAALVAAVEELHGRLSIVSRERIRDELDKLMVTAE
                     VAVGLWFLVETGLAGEMLPELPALALEQDPIHRHKDVLAHTIAVVEKTRPDRLLRLAA
                     LFHDVGKPRTRTFEGGGVSFHHHEVVGARMTRDRMRELRYPAEDVQTVSRLVELHLRF
                     HTYKMGWTDRAVRRYVRDAGPLLNRLNELTRCDCTTRNEARARALSKRMDELEARVAE
                     LRAQEELDAIRPDLDGRQVMEVLGVPPGPAVGRALAFLLELRLDEGPLGEEEATRRLQ
                     AWWAAGGPAA"
     gene            complement(3468..4208)
                     /locus_tag="VF954_00355"
     CDS             complement(3468..4208)
                     /locus_tag="VF954_00355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9529554.1"
                     /translation="MPTDNAAAWDRHSAAYQAGAQLPTDTAHYGPDIATEADLKLLGD
                     LRGKRVLELGCGGGQCSIAFAKQGATAIGIDFSVEQLAFARRLVDREGVRVELRHGDL
                     ADLAFLRADSIDAVFSAYAFGYVEDLGRVFRQVHRVLKVGTPLVFSVPHPAYDMIDDD
                     HPDEPMLVRRSYFDKSPIDYEWNGIPFTDYHHSISELYRGLVRSSYRVDAILEPEPRP
                     GGPRSPDWRETFRYVPRTLVIRARKEGS"
BASE COUNT          590 a         1516 c         1645 g          579 t
ORIGIN      
        1 tgacggcggc caggcgggcg cgggggcgca tcaggggagc agcccggcgg acaggaccga
       61 caggccgaag ggctcgtggg cgaagccgag gcgctcgtag agggcgaggg cggcggcgtt
      121 ctccctctgg gtgttgacca ccacccgctc ggcgccccac cgccgcagcc agcgcaggcc
      181 gtcgacgatc agggccgcgc cgaggccccg cccctgatgg cctggatgca cggccacgcg
      241 ctggaggaac ccgtgccggg cggcgcgccc ggtgatggca tagccggcga tggcgccggt
      301 gccgggggtg gcgacacggt accgggcgcg gggggtggcc tcgagggcct cctccaggcc
      361 ggcgtcgtcg agctgccaga aggggggaaa ggccacccgg tcgacctcga gcacgtcgtg
      421 gcggtccgac gcccgggccc tgcggaaggc gacgcccgga ggtcgcgggg cggcgggcag
      481 ctgctcgagg ttgcgcccga ggaggtggag ccgctcggtg acccggaagc cggccgagag
      541 gaaggcgtcc tgctcggcgg gcgccagcgc cccggtcacc acctgggaga agccgcgggc
      601 gctgagctgg tccatgcacc tgcggacgaa cgccgccgag ggcaccggcg cgtcggcgac
      661 cggcgccagg taggcgacgc ggtcctggcc ccgccacggg ccgatccgcg ctcgctcctg
      721 gccccagcga aggacttgga cgtcgggcgc gctcactcgc tcgtcgaggg tagtggccgc
      781 ccggtgtgcc ggcaggcatc ggtgacgggg cgcctcaggt gcgggccggc ccgaaccgat
      841 acacacaggg tgctcgatct gacggagttg ctgcgctaca cggtcgagca gggtggctcg
      901 gacctgcacg tgaaggtggg ctcggtcccg cacgtgcgcg tcgacggcca tctgatgcct
      961 gcgccgttcg accgggtggc ggcggccgag gccgagcgca tgggcttcac catcctgccg
     1021 aaggagcggg ccgacgagtt cgtggagacg gcggaggccg acttcgccca cagcgtgccc
     1081 ggactgggcc ggttccgcgt caacatcttc cgccagcgtg ggtcggtggg cttggtgttc
     1141 cggcgcgtcc tgcccggcat cccgtccttc gagtccctcg gcctgccggg agtggtccgc
     1201 cgcctggccg acgagaaccg cggcctggtg ctcgtcaccg gccccaccgg ctcgggcaag
     1261 accaccacca tcgcggcgat gatcgaccac atcaacgcca ccaaggcggt caacatcgtg
     1321 accatcgagg accccatcga ggtcctgcat gtcgacaaca aggcgctggt caaccagagg
     1381 gagatcggca gcgacacccg ggactactcg atggccatga agcgggtgct gcgccaggac
     1441 cccgacgtca tcttcgtcgg tgagatgcgc gaccccgaga cggtggccgc ggccctcgcc
     1501 gccggtgaga cggggcacct ggtgttctcc acgctgcaca cgaccgacgc ggtcgagacc
     1561 gtcaaccgca tcgtggactt cttccccccc taccagcagc ggcaggtccg cctctcgctg
     1621 gcctcgtcgc tgcgcggcgt catctcccag cgcctgctgg agcgggccga ccgccgcggc
     1681 cgggttccgg cggtggaggt gctggtgatg acggggcggg tgttcgaccg ggtcatcgac
     1741 agcgacaccc aggagaccat cgaggaggtc atcgccgacg gcgagtacta cggcatgcag
     1801 accttcgacc agagcctgtt cgggctctac aaggacggcc tggtgagcct ccgcgacgcc
     1861 atggccgcct cgacgcaccc ccacgacctg cgcatcaagc tccagcaggc cggcgtcctg
     1921 gccgtttaaa cgcacggggc cgggcggggc ctccccggta gcctcgggcg gcgtgatccc
     1981 cgaccgcttc cgcccgctcg tggagctgac gtcgtcgctg gcggcgcgct tccaggcggc
     2041 cggcttcagc ctctacctgg tgggcggggt ggtgcgcgac cacatcctgg gcgtggccgc
     2101 cgagggctcg gacctggatt tcaccaccga tgcccggccc gacgacgtcg aggggctcct
     2161 gtcgggatgg gccgagtcgg tgtggctcca gggcaagagg ttcggcacgg tcggggcgtg
     2221 gcgcgggggc ctgcgccacg agatcacgac gcaccgggcc gaggcctacg ccgccgactc
     2281 gcgcaagccg gaggtggctt tctccgacga cgtcgcggtc gacctgtccc ggcgggactt
     2341 cacggtcaac gccatggccc ttcggctgcc cgacctgacc ctggtcgacc ccttcgacgg
     2401 ggtggccgac ctggccgccg ggcgcctgcg gacccccctc agccccgagg agtccttcaa
     2461 cgacgacccc ctgcggatgc tgcgcgccgc ccgcttcatg gccggcttcg gcctgaagcc
     2521 cgacgccgcc ctggtggccg cggtcgagga gctgcacggc cggctgtcca tcgtgtcgcg
     2581 cgagcgcatc cgcgacgagc tggacaagct gatggtgacg gccgaggtcg ccgtcgggct
     2641 gtggttcctg gtcgagaccg ggctggctgg cgagatgctg cccgagctgc ccgccctggc
     2701 cctggagcag gaccccatcc accggcacaa ggacgtgctg gcccacacca tcgccgtggt
     2761 ggagaagacg cggcccgacc ggctgctgcg gctggcggcc ctgttccacg acgtgggcaa
     2821 gccgcggacc aggacgttcg agggcggcgg ggtgagcttc caccaccacg aggtggtggg
     2881 ggcgcggatg acccgcgacc gcatgcgcga gctgcgctat cccgccgagg acgtccagac
     2941 ggtcagccgg ctggtggagc tgcacctgcg cttccacacc tacaagatgg gctggaccga
     3001 ccgggccgtg cggcgctacg tccgcgacgc cggtcccctg ctcaaccggc tcaacgagct
     3061 gacccgctgc gactgcacca cccgcaacga ggcccgggcc cgggccctgt ccaagcgcat
     3121 ggacgagctg gaggcccggg tcgccgagct gcgcgcccag gaggagctcg acgccatccg
     3181 ccccgacctg gacggccggc aggtcatgga ggtgctcggc gtcccgccgg ggccggccgt
     3241 cgggcgggcc ctggccttcc tgctcgagct gcgcctcgac gaggggcccc tcggagagga
     3301 ggaggccacc cgccggctcc aggcgtggtg ggcggccggc ggcccggccg cctagaaagg
     3361 ccccggccgg gctgatgggc tgatgggcac ggccgccggc ggggttgggg tccccgccgt
     3421 ggggaggagg ccggggttgg ggccccggcc gggccagtca gctcagctca gctcccctcc
     3481 ttccgggccc gaatgacaag ggtccggggg acgtagcgga aggtctcgcg ccagtccggg
     3541 ctgcggggcc cgccgggccg gggctcgggc tcgaggatgg cgtcgacccg gtagctggag
     3601 cgcaccagcc cgcggtacag ctcggagatg gaatggtggt agtcggtgaa ggggatgccg
     3661 ttccactcgt agtcgatggg cgacttgtcg aagtacgagc gccggaccag catcggctca
     3721 tccgggtggt cgtcgtcgat catgtcgtag gccgggtgcg ggacgctgaa cacgagcggg
     3781 gtgcccacct tcagcacgcg gtggacctgg cggaagaccc ggcccaggtc ctcgacgtag
     3841 ccgaaggcgt aggccgagaa caccgcgtcg atgctgtcgg cccgcaggaa ggccaggtcg
     3901 gccaggtcgc cgtggcgcag ctcgacccgc actccctccc ggtcgacgag gcggcgggcg
     3961 aaggcgagct gctcgaccga gaagtcgatg ccgatggcgg tggcgccctg cttggcgaag
     4021 gcgatcgagc actgcccgcc tccgcagccc agctccagga cgcgcttgcc cctcaggtcg
     4081 ccgagcagct tgagatcggc ctcggtggcg atgtcgggcc cgtagtgggc cgtgtcggtg
     4141 ggcagctggg ctccggcctg gtaggcggcg gagtgccggt cccacgcggc cgcgttgtcg
     4201 gtcggcatgc ccgcgatcat ggccgatccc gggcggtggg gtgcgctagt ccccccgggc
     4261 gaagtcctcc acggcctggc gggcgtcgtc gtcgcggtac tggacgggcg gcgacttcat
     4321 gaagtaggag
//
LOCUS       DASWGG010000021         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_333704, whole genome shotgun sequence.
ACCESSION   DASWGG010000021 DASWGG010000000
VERSION     DASWGG010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333704"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1835)
                     /locus_tag="VF954_00360"
     CDS             complement(<1..1835)
                     /locus_tag="VF954_00360"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX9529555.1"
                     /translation="MGGGRIGRRMVGVAAACSAMSLVGLGLSATMPAASAAAASLPAV
                     SPGRVTTFLNHFDSTTLDVATEAPKRRVIALNAWDYPYIPAIKAANPATLVLVYKDLS
                     STRDFSCHNGVDDAELPTGVGYCYANANHPEWFLTDSSGNRLTYGGYPGHWQMDVGNA
                     AYQQAWLANVRADVTAHGWDGVLMDNALTVADTYHTGVTPALYPTDTALQTAYRAMLA
                     LVSPGLRQAGLLTFANIGDARLFPGLWGDWISLLDGASQENFANWSSTVGSGYMWDWG
                     SDGWRSMVDELVTAATEGKIGLVTGGGVANDNDALRYDVATYLLGNDGRSIFSFTDPE
                     ATIPEMSWDLGAPSGAYQSLSNHIYRRQFSGGTVVVNASQSGTQTVSLGGTYLDVNGN
                     PVSSVTLPATRAAILRSSGTTTSTTAPPTTTSTTAPPTTTSTTAPPATTATTVATSTT
                     GTTAPPNPKPHKKASALYLMSANGGVMAAGAPSTLGSAAGQTLNQPVVGGAATTDDGG
                     YWMVAQDGGIFAFGDAPFYGSTGAIRLNQPIVGMAATPTGSGYWLVARDGGIFAFGDA
                     RFFGSTGAIALNRPIVAMAATPTGRGYWLTASDGGIFAFGDAAFFG"
     regulatory      complement(1897..1970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2055..3185)
                     /locus_tag="VF954_00365"
     CDS             complement(2055..3185)
                     /locus_tag="VF954_00365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9529556.1"
                     /translation="MTQGPVVFVLTQSRGGPVDLTVGLAREIAGRVGGPEVRVIGPNL
                     ESSAGDVGDLLVPVTIASKGDTRGARELRRQVHEASPWIVHAQDRRAALFVASVARPR
                     APVVVTMHGVPDGAARWWVQGGPLHHQPVAPGAAAVLMGDALIGRVVDAVVAPSQTMA
                     RFLRDRLRIPARRIEVITNGVAPRDARPLADRARVYISVGSFAAIKGMRSLLRAFAEV
                     VRTHPDVELRLIGDGDERAACERLVAELGFGAHVEFAGYRLDVAEQLRLADVFVLPSL
                     NENLPLALLEAMATGLACVAADVGAVREALRTGAGLVVPPGDERALAAALARLADEPG
                     LGARLGGAAAAAAAQFTTARAADEHLALWRSLTTPGANIQRP"
     gene            complement(3182..>3569)
                     /locus_tag="VF954_00370"
     CDS             complement(3182..>3569)
                     /locus_tag="VF954_00370"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9529557.1"
                     /translation="PHPAQRLGEVTVLPATDRAGVRRCIEGAAAVVVPLLRPDRTAGQ
                     LAVLDAFASGRGVIATRAQGTEDYVSHDNGILVPVGDATALRRALLDVGDPPRAAELG
                     AGARRSTAGPLALERFVRDVHGVAAR"
BASE COUNT          509 a         1229 c         1305 g          526 t
ORIGIN      
        1 ccgaagaacg ccgcgtcgcc gaaggcgaag atcccgccgt ccgacgccgt gagccagtaa
       61 ccccggcccg tcggcgtggc cgccatcgcc acgatcggcc ggttcagggc gatggcaccg
      121 gtcgacccga agaaccgggc gtcgccgaag gcgaagatcc ccccgtcgcg ggcgaccagc
      181 cagtagccgg agccggtggg cgtggcggcc atgcccacga tgggctggtt gaggcggatg
      241 gccccggtag agccgtagaa cggggcgtcg ccgaaggcga agatcccgcc gtcctgcgcc
      301 accatccaat agccgccgtc gtctgtggtg gccgctccgc cgacgaccgg ttggttgagc
      361 gtttgccctg ctgcggaccc gagcgtcgag ggcgccccgg cggccatgac cccgccgttg
      421 gccgacatca ggtaaagcgc cgaggccttc ttgtggggct tagggttggg cggcgccgtg
      481 gtgccggtcg tgctcgtcgc cacggtggtg gcggttgtgg ccggcggcgc cgtggtggac
      541 gtggtggtcg ggggcgcggt cgtggacgtc gtggtcgggg gcgcggtcgt ggacgtggtg
      601 gtgcccgacg agcggaggat ggccgcccgg gtggccggca gcgtcaccga cgagaccggg
      661 ttgccgttga cgtccaggta ggtcccgccg agggagacgg tctgggtgcc gctctgggag
      721 gcgttcacga ccaccgtgcc cccgctgaac tggcgccggt agatgtggtt gctcaacgac
      781 tggtacgcgc cggacggagc gccgagatcc cagctcatct ccgggatcgt cgcctccgga
      841 tcggtgaagc tgaagatcga acgcccgtcg ttgccgagca ggtaggtcgc cacgtcgtag
      901 cggagggcgt cgttgtcgtt ggccacgcca ccgccggtca ccagtccgat ctttccctcc
      961 gtcgccgccg tcaccagctc gtcgaccatg gatctccagc cgtcgctgcc ccagtcccac
     1021 atgtacccgg agccgacggt cgagctccag ttggcgaagt tctcctggga tgcaccgtcg
     1081 agcagcgaga tccagtcgcc ccaaaggccc gggaagagcc tggcgtcacc gatgttggcg
     1141 aaggtgagca ggccggcctg ccgcaggccc ggggagacca aggcgagcat ggcccggtac
     1201 gcggtctgca aggcggtgtc ggtcggatag agcgccgggg tcacgccggt gtggtaggtg
     1261 tcggcgacag tcagcgcgtt gtccatgagg acgccgtccc agccgtgcgc cgtcacatcg
     1321 gccctgacgt tggccagcca cgcctgttgg tacgcggcat tgccgacgtc catctgccag
     1381 tggcccgggt agccgccgta ggtgaggcgg ttcccagagc tgtcggtcag gaaccactcg
     1441 ggatggttgg cgttggcgta gcagtagccc acgccggtgg gtagctcggc gtcatccacc
     1501 ccgttgtggc aggagaagtc ccgggtggac gaaaggtcct tgtacacgag caccagcgtg
     1561 gcgggattgg cggccttgat ggccgggatg tagggatagt cccatgcgtt gagggcgatc
     1621 acccggcgct ttggcgcctc ggtggccacg tccagcgtcg tgctgtcgaa gtggttgagg
     1681 aacgtggtca cccgaccggg gctcacggcg ggcagggatg ctgcagcggc tgacgcggcc
     1741 ggcatggtag cgctgaggcc caggccgacg agcgacatcg ccgagcacgc cgcggccacg
     1801 cccaccatcc gccttccgat ccggcctccg cccatcgtgg ttccttccca gcgggaagcc
     1861 gaaggcgcgt acgaacagat ctcgtccttc ctgcgattcg gcaaccggct gactccgcga
     1921 gtcccttggt tttgcgtccc cgcctcacga cgggtttgcc tttgtcactc tcagtgctct
     1981 gtaccagggg tatcggcccg ttccgaccgg gacctgagca agggatccgg gaaaatggac
     2041 cgtgtgtggg cgggctacgg acgttgaata ttcgcgccag gagtggtcag cgatcgccac
     2101 agcgccaggt gctcatcggc ggcccgggcc gtcgtgaact gcgccgccgc ggccgccgcg
     2161 gccccgccga ggcgggcacc caacccgggc tcgtccgcca accgggccag agcggccgcc
     2221 agcgcgcgtt cgtcaccggg gggcacgacc agcccggcgc cggtcctcag cgcctcccgc
     2281 accgcgccga cgtctgcggc gacgcatgcc agccctgtgg ccatggcctc gaggagggcg
     2341 aggggcaggt tctcgttgag tgaggggagt acgaatacgt ccgccagtcg aagttgctcg
     2401 gccacgtcga gtcggtaccc ggcgaactcc acgtgggcgc cgaagcccag ctcggcgacg
     2461 aggcgttcgc aggcggcgcg ctcgtcgccg tcgccgatga gcctgagctc gacgtccggg
     2521 tgggtgcgca ccacctcggc gaaggcccgg agcaagctgc gcatcccctt gatggcggcg
     2581 aaggacccca cggagatgta tacccgggcc cgatcggcca acggccgcgc gtcccgcggc
     2641 gccacgccat tcgtgatcac ctcgatccgt cgggccggga tgcggagccg gtcccgcagg
     2701 aaccgcgcca tcgtttggga tggcgccacc acggcatcca cgacccggcc gatgagggcg
     2761 tcgcccatca acacggcggc cgcaccgggc gccacaggct ggtggtggag gggaccgcct
     2821 tgcacccacc atcgggccgc gccgtccggg acgccgtgca tcgtgaccac caccggggcg
     2881 cgcggtcgtg cgacggaggc gacgaagagg gccgcccggc ggtcctgggc gtggacgatc
     2941 cacggtgacg cctcgtgcac ctgcctgcgc agctcccgcg cgcccctggt gtcgcccttg
     3001 gacgcgatcg tgaccggcac cagcaagtcg cccacgtccc cggcgctgct ctctaggttt
     3061 ggcccgatga cgcggacctc cggtccgccg acccggccgg cgatctcccg ggccagcccc
     3121 accgtcagat cgaccggtcc tccgcgggat tgcgtgagca cgaagaccac cggtccctgt
     3181 gtcaccgggc tgccactccg tggacatcgc gaacgaagcg ttcgagggca agtgggcccg
     3241 cggtcgagcg gcgcgcaccg gcgcccaact cggcggctcg cggtgggtca ccgacgtcga
     3301 gcagcgctcg gcgaagcgcg gtggcgtcgc ccacggggac gaggatcccg ttgtcgtgcg
     3361 agacgtagtc ctcggtcccc tgcgcacgtg tggcgatcac gccgcgcccc gacgcgaagg
     3421 cgtcgaggac ggcgagttgg ccagccgtcc gatcgggcct gagcaagggg accacgaccg
     3481 ccgcggcccc ttcgatgcag cgtcgcacgc cggcccggtc ggttgccgga agcacggtga
     3541 cctcgccgag ccgctgggct gggtgcggc
//
LOCUS       DASWGG010000022         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2601599, whole genome shotgun sequence.
ACCESSION   DASWGG010000022 DASWGG010000000
VERSION     DASWGG010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2601599"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VF954_00375"
     CDS             <1..844
                     /locus_tag="VF954_00375"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX9529558.1"
                     /translation="AMVIGLLQGVTELFPISSLGHSVLVPAVIGGSWHHLVTDQSSAE
                     SPYLAFLVGLHFATALALLAYFRAEWARIIRAFFRSLRDRRIEGPDARLAWLLAVATV
                     PAGLTGLAFEHTFRTVFAKPTAAAAFLTVNGLVLLAGERFRRGAGGGHRRLDTLELKE
                     AGVVGVAQVLALLAGISRSGITMVAGLARGLDHEDAARFSFLLATPIILAAGLYKAPD
                     LLGHNGAGIRGQVVAGSLVAAAAAYVSVRFLVRYFETRNLRPFGIYCIVAGATLTVYF
                     GLVA"
     gene            964..1764
                     /locus_tag="VF954_00380"
     CDS             964..1764
                     /locus_tag="VF954_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529559.1"
                     /translation="MAPDTSSSRSSSSLSGSAARLVRLCTPVPAPPERACALCHGHLP
                     SGAGALCSGCATVSRQVAACPVVAPVSLYAVGGGLHRVLRGYKDDPLPSVRLSCRRQL
                     AQLLGGFLADHGPCVAEAAGRRWDGVAVVPSSAGRPGPHPLGRVVAEALGGKGELYEV
                     LAPGPVRPDHRRARRGGFVVSGAVRGARLLLVDDTWTSGARAQSAACALREAGALVAA
                     VVPIGRVGDPGFSPEASADWERWRGRAFDPADCCVGGHARARPSGRVA"
     gene            1858..>2193
                     /locus_tag="VF954_00385"
     CDS             1858..>2193
                     /locus_tag="VF954_00385"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX9529560.1"
                     /translation="MTDANISYLAAFGGGIVSFLSPCVLPLVPAYLSMITGLEVSEVQ
                     QEGRQHLGRIARDTGLFVAGFSVVFVLLGLSASAAGLTLVHHHLLLTRISGALVVAMA
                     AFLAGSLVLK"
BASE COUNT          208 a          835 c          795 g          355 t
ORIGIN      
        1 ggcgatggtc atcgggctgc tccagggcgt gaccgagctg ttccccatct ccagcctggg
       61 ccattccgtg ctcgtgcccg cggtgatcgg cggcagctgg caccacctcg tcaccgacca
      121 gtcgagcgcc gagtcgccgt acctggcctt cctcgtcggc ctgcacttcg ccacggccct
      181 cgccctcctc gcctacttcc gggccgagtg ggcccggatc atccgggcct tcttccggtc
      241 gctgcgggac cgcaggatcg aaggtcccga cgcccgcctg gcgtggctgc tggcggtggc
      301 gacggtcccg gccgggttga ccgggctggc gttcgagcac accttccgca cggtgttcgc
      361 caagcccacc gctgccgccg ccttcctgac cgtcaatggg ctggtgctgc tcgccggcga
      421 gcggttccgc cgaggcgcgg ggggtggcca tcgccggctc gacacgctcg agctcaagga
      481 ggccggcgtc gtgggggtgg cccaggtctt agccctcctg gccggcatca gccggtccgg
      541 catcaccatg gtggccggcc tggcccgggg gctggaccac gaggacgcgg cccgcttctc
      601 gttcctcctg gccaccccga tcatcctggc cgccgggctg tacaaggcgc cggacctcct
      661 gggccacaac ggcgccggca tccggggcca ggtggtggcc ggcagcctgg tggcggcggc
      721 ggcggcctac gtctcggtca ggttcctcgt gcggtacttc gagacccgca acctgcggcc
      781 cttcgggatc tactgcatcg tcgccggcgc cacgctcacc gtgtacttcg ggctggtggc
      841 ctgagcctgg acggaatacc atgagatgat ctcgcacgcg aagccggcgg gcctcgcggc
      901 tgcgatctct ccccatttgc gcctcacggc tgcctgcgaa cacggctcct cccgtgaggt
      961 ggcatggcgc ccgacaccag ctcctcccgc agctcctcca gcctctccgg cagcgcggcc
     1021 cggctggtcc ggctctgcac ccccgtgccc gcgccgcccg agcgggcctg cgccctgtgc
     1081 cacggccacc tcccgagcgg cgccggcgcc ctgtgctccg gctgcgccac cgtctcccgc
     1141 caggtcgcgg cctgcccggt cgtggcgccc gtctcgctct acgccgtcgg cggcgggctc
     1201 caccgggtgc tgcgcggcta caaggacgac cccctcccgt cggtgcgcct gtcgtgccgg
     1261 cgccagctcg cccagctgct gggcgggttc ctggccgacc acggaccgtg cgtggcggag
     1321 gcggcggggc gccgttggga cggggtcgca gtcgtcccgt cgtcggccgg ccggccgggc
     1381 ccgcatcccc tgggtcgggt cgtggccgag gcgctcgggg ggaaagggga gctgtacgag
     1441 gtcctggccc cgggccccgt ccggcccgac caccgccggg cccggcgcgg cggcttcgtg
     1501 gtgagcggcg cggtgcgagg cgcccggctg ctgctcgtcg acgacacctg gacctccggc
     1561 gcccgggccc agagcgcggc ctgcgccctt cgcgaggccg gggcgctcgt ggccgcggtg
     1621 gtgccgatcg ggcgggtggg cgatcccggc ttcagcccgg aggcctccgc cgattgggag
     1681 aggtggcggg gtcgggcctt cgacccggcc gactgttgcg tcgggggcca cgcccgggcc
     1741 cggccgtcgg ggagggtggc gtgagggctc gtatgcttct gccatgcccg gtgctcccgc
     1801 catgcccggt gccgatgtgg ggaaagctgg ctccgtcggt tccccgccgg ccggctcgtg
     1861 accgacgcca acatcagcta cctggctgcg ttcggtggcg gcatcgtctc ctttctcagc
     1921 ccgtgcgtgc tgccgctggt gcccgcctac ctgtcgatga tcaccggtct cgaggtctcc
     1981 gaggtccagc aggagggccg ccagcacctg gggcgcatcg ccagggacac cgggctgttc
     2041 gtggccggct tcagcgtggt gttcgtgctg ctcggcctgt cggcgtccgc cgccggcctg
     2101 acgctcgtgc accaccacct cctgctgacg cggatctcgg gcgcgctggt cgtcgccatg
     2161 gccgccttcc tggccggctc gctggtcctg aag
//
LOCUS       DASWGG010000023         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2602557, whole genome shotgun sequence.
ACCESSION   DASWGG010000023 DASWGG010000000
VERSION     DASWGG010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2602557"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /gene="purB"
                     /locus_tag="VF954_00390"
     CDS             complement(<1..1095)
                     /gene="purB"
                     /locus_tag="VF954_00390"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX9529561.1"
                     /translation="MANVLAARYASPEMTVLWSAEGRVRLERQLWIAVLEAQREAGIE
                     VADGVIEAYRAVVDKIDLDAIDARERVTKHDVKARLEEFSALAGHEQVHKGLTSRDLT
                     ENVEQLQVRDALALVRDRMVAALVRLARLAASNSEVVMAARTHNVPAQATTLGKRFAN
                     AGQEVLAALARVESLLDGYPLRGLKGPVGTQQDQLDLLDGDLDRLDRLEAAVASHLGF
                     SSALANVGQVYPRSLDLDVVAALVQAGAGPSSLATTIRLMAGHELATEGFLPGQVGSS
                     AMPHKMNTRSCERVNGLLTVLRGHLTMTAGLAGDQWNEGDVSCSVVRRVVLPDAFFAA
                     DGLYETFLTVLDGFAAYPAVIEHELARYLPF"
     gene            complement(1152..1598)
                     /gene="purE"
                     /locus_tag="VF954_00395"
     CDS             complement(1152..1598)
                     /gene="purE"
                     /locus_tag="VF954_00395"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX9529562.1"
                     /translation="MKVGVLMGSPGDREKMAPAVSMLQRFGVEAEERVLSAHRNPEEV
                     ASLARAARDEGFAAFICGAGMAAHLAGVVAAHTTLPVVGVPLSGGALNGLDALYSTVQ
                     MPRGVPVASVAIDGSANAAILVVEMLAITDEALATRLDEFRAGGAR"
     gene            complement(1595..2272)
                     /locus_tag="VF954_00400"
     CDS             complement(1595..2272)
                     /locus_tag="VF954_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEX9529563.1"
                     /translation="MALAPAQDFKRVENGDRGPNTGGMGAYSPVPVAGKDVVDEVMDA
                     FVGPTLRELRRRGIDYRGVLYAGLMLTPEGPKLIEYNVRFGDPEAQVVLPRMEGDLAR
                     LLAGAASGSLAAVAGEARFGADAAVTVVCCAGGYPGGPLRTGVPISGLDAAAAVPGVT
                     LFHAGTALSGSGPLVVAGGRVLGVTALAPTIAGARARAYAAAGHLSWEGIHYRGDIAE
                     AAAGEAA"
BASE COUNT          330 a          866 c          907 g          275 t
ORIGIN      
        1 gaagggaagg tagcgggcca gctcgtgctc gatcacggcc gggtaggcgg cgaagccgtc
       61 cagcacggtc aggaacgtct cgtacaggcc gtccgccgcg aagaaggcgt cgggcagcac
      121 gacgcggcgc acgaccgagc agctgacatc gccttcgttc cactggtcgc cggccagccc
      181 ggccgtcatg gtgaggtggc cccgcagcac ggtcagcagg ccgttgacgc gctcgcagga
      241 tcgggtgttc atcttgtggg gcatggcgga ggagccgacc tgtccgggca ggaagccctc
      301 ggtcgccagc tcgtggccgg ccatgagccg gatggtggtc gccaggctgg acgggccggc
      361 gccggcctgc acgagggcgg cgaccacgtc gaggtcgagg gagcgggggt agacctggcc
      421 cacgttggcc agcgccgacg agaagcccag gtgggaggcg accgcggcct ccagccggtc
      481 gaggcggtcg aggtcgccgt cgagcaggtc gagctggtcc tgctgggtgc ccaccgggcc
      541 cttgagaccg cggagcgggt agccgtcgag cagcgactcc acccgggcga gggcggcgag
      601 gacctcctgg ccggcgttgg cgaagcgctt gccgagggtg gtggcctggg cagggacgtt
      661 gtgggtccgc gccgccatga ccacctcgga gttggaggcg gccaggcggg ccaggcggac
      721 cagggccgcg accatgcggt cgcgcaccag ggccagggcg tcgcggacct ggagctgctc
      781 gacgttctcg gtgaggtcgc gggaggtgag gcccttgtgg acctgctcgt ggccggcaag
      841 ggcggagaac tcctcgaggc gggccttgac gtcgtgcttg gtgaccctct cgcgggcgtc
      901 gatggcgtcg aggtcgatct tgtcgaccac cgcccggtag gcctcgatca ccccgtcggc
      961 cacctcgatg ccggcctcgc gctgggcctc gagcaccgct atccagagct ggcgctctag
     1021 gcgcacccgg ccctcagccg accacagcac ggtcatctcc gggctggcgt agcgggccgc
     1081 caggacgttg gccacgggct ccttcgccag tcgcgcgccg gggcgcgcgc cggggcgcgc
     1141 ccgacccggg ctcaccgcgc ccccccggcc cggaactcgt ccagcctggt ggccagggcc
     1201 tcgtcggtga tggcgagcat ctcgaccacc aggatggcgg cgttggccga cccgtcgatg
     1261 gccacgctgg cgaccgggac gccccgcggc atctgcaccg tcgagtacag agcgtcgagc
     1321 ccgttgaggg cgccgcccga gagggggacg ccgacgacgg ggagggtggt gtgggccgcc
     1381 accacgccgg ccaggtgggc ggccatgccg gccccgcaga tgaaggccgc gaagccctcg
     1441 tcgcgggcgg cccgcgccag cgaagccacc tcctcggggt tgcggtgggc cgacagcacc
     1501 cgctcctcgg cctccacgcc gaagcgctgg agcatgctga cggccggcgc catcttctcc
     1561 cggtcgccgg gcgagcccat cagcacgccg accttcacgc cgcctccccg gccgcggcct
     1621 cggcgatgtc gccgcggtag tggatgccct cccacgacag gtgcccggcc gcggcatagg
     1681 cccgggcccg ggccccggcg atggtcgggg ccagggcggt gacgccgagc acccgccctc
     1741 ccgccaccac caggggaccg ctcccggaga gcgcggtgcc ggcgtggaac agggtgacgc
     1801 ccgggacggc ggccgcggcg tcgaggccgg agatgggcac cccggtgcgc agcggcccgc
     1861 ccgggtaccc gccggcgcag cacaccaccg tgacggccgc gtcggcgccg aagcgggcct
     1921 cgccggcgac ggccgccagc gagcccgacg ccgccccggc cagcagccgg gccaggtcgc
     1981 cctccatgcg cggcagcacg acctgggcct cggggtcgcc gaagcgcacg ttgtactcga
     2041 tcagcttggg gccctcgggg gtgagcatca gcccggcgta gagaacgccc cggtagtcga
     2101 tgccccggcg gcgcagctcg cgcaaggtgg gcccgacgaa ggcgtccatc acctcgtcca
     2161 ccacgtcctt gccggccacg ggcaccggcg agtaggcgcc catcccgccc gtgttgggcc
     2221 cccggtcgcc gttttcgacg cgcttgaagt cctgggcggg agccagcgcc accgccgcac
     2281 ccgagcccga gcccgagccg gagcccgacg aggaagagga agaagaagaa gaggaagcca
     2341 aagacgccga agccgccgct gcctacaagg ccggcctc
//
LOCUS       DASWGG010000024         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2603597, whole genome shotgun sequence.
ACCESSION   DASWGG010000024 DASWGG010000000
VERSION     DASWGG010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2603597"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VF954_00405"
     CDS             <1..319
                     /locus_tag="VF954_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529564.1"
                     /translation="GMREPLPLYCKTSAAYAAARLTGMGADVTDAARREWEVGLFGEQ
                     LDAEHQLVYDGLRSLDEVEEEQPPGTETGDGWDPGEATRFGRLACRMWSDVLAREKVA
                     VDG"
     gene            312..>1653
                     /locus_tag="VF954_00410"
     CDS             312..>1653
                     /locus_tag="VF954_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9529565.1"
                     /translation="MDDLRPFDLLGPLPGPGVTVLEASAGTGKTFTIAALAVRFVASG
                     APLDRVLIVTFTRLATSELRDRVRHRLVTAAEALRAITEGEPVPPGDELVAFLATGTP
                     EEVSCRRDRLHSAVANFDAATIATTHGFCQQVLQGLGVLGDAPQEATVLEDPSDLVTE
                     VVDDRYTRHVLELGSVPFDRRQALAIGRCAVANPQAPIEPRHAEPSTPSGLRRRLAKT
                     TRDELERRKRAGGLLTYDDLVTRLHDTLTDDKRGAAACARLRERYQVALVDEFQDTDP
                     VQWGILARAFGDGQATLVLIGDPKQAIYGFRGADVYAYLDAARRADHQATLSTNFRSD
                     ADYLAAVEALLGQARLGHNGIPFRAVEATADHRTPGLQGAPDGAPLRFRVLDRSSPAV
                     RKVAGGAAAVGPARQLVAADVAGDIAALLRSGALVRPVPARDAATEVRAGDVAVL"
BASE COUNT          210 a          648 c          590 g          205 t
ORIGIN      
        1 cgggatgcgc gagcccctcc cgctgtactg caagacctcg gctgcgtacg cggccgcccg
       61 cctgaccggg atgggcgcgg acgtgacgga cgcggcccgg cgggaatggg aggtcggcct
      121 cttcggcgag cagctcgacg ccgagcacca gctcgtctac gacgggctcc ggagcctcga
      181 cgaggtcgag gaggagcagc ctccgggcac cgagacgggc gacgggtggg accccggcga
      241 ggccacccgc ttcggccggc tcgcctgtcg catgtggtcc gacgtcctcg cccgcgagaa
      301 ggtggccgtc gatggatgac ctgcggccct tcgacctgct ggggccgctc cccgggcccg
      361 gcgtcaccgt gctcgaggcg agcgccggca ctgggaagac cttcacgatc gccgctctcg
      421 cggtccgctt cgtggcctcc ggagcaccgc tcgaccgggt gctgatcgtg accttcaccc
      481 ggctggcgac cagcgagctg cgggaccgcg tccggcaccg gctggtgacc gcggccgagg
      541 ccctccgcgc catcaccgag ggcgagcccg tcccgcccgg tgacgagctg gtcgccttcc
      601 tggccaccgg cacgccggag gaggtgtcct gccgtcgcga ccggctgcac tcggccgtcg
      661 ccaacttcga cgccgccacc atcgccacca ctcacggctt ctgccagcag gtcctccagg
      721 gcctcggcgt gctgggcgac gccccccagg aggccaccgt gctcgaagac cccagcgacc
      781 tggtcacgga ggtcgtcgac gaccggtaca ccaggcacgt cctcgagctg gggagcgtgc
      841 cgttcgaccg gcgccaggcc ctcgccatcg ggcggtgcgc ggtggccaac ccgcaggcgc
      901 ccatcgagcc gcgccacgcc gagccgtcca cgccgtccgg cctgcgccgg cggctggcca
      961 agacgacccg cgacgagctc gaacgccgca agcgtgcagg tggcctgctc acctacgacg
     1021 atctggtcac ccgcctccac gacacgctga ccgacgacaa gcggggcgcg gcggcctgcg
     1081 cccggctgcg ggagcgctac caggtcgcgc tggtcgacga gttccaggac accgaccccg
     1141 tccaatgggg catcctggcc cgcgccttcg gcgacgggca ggccaccctg gtgctgatcg
     1201 gcgatcccaa gcaggccatc tacggcttcc gcggggccga cgtctacgcc tacctcgacg
     1261 cggcccggcg cgccgaccat caggcgaccc tgagcaccaa cttccgcagc gacgccgact
     1321 acctggcggc cgtcgaagcc cttctcggcc aggcccgcct tgggcacaac gggatcccgt
     1381 tccgcgccgt ggaggccacc gccgatcacc gcacgcccgg gctccagggc gcccccgacg
     1441 gcgcgccgct gcgcttccgg gtgctcgacc ggtcctcccc cgccgtccgg aaggtggcgg
     1501 gcggtgcggc ggccgtcgga cccgcccgcc agctggtggc ggcggacgtg gcgggcgaca
     1561 tcgccgcgtt gctgcgatcg ggggccctcg tgcggcccgt ccctgctcgg gacgcggcga
     1621 cggaggtgcg ggccggggac gtcgccgtgc tgg
//
LOCUS       DASWGG010000025         3900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_336130, whole genome shotgun sequence.
ACCESSION   DASWGG010000025 DASWGG010000000
VERSION     DASWGG010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336130"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VF954_00415"
     CDS             complement(<1..507)
                     /locus_tag="VF954_00415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9529566.1"
                     /translation="MATKLAAAEIGELDPYKFMAVIGKRVIHPGGRASTRALLDRAGI
                     TADSRVLDVGCGVATTAVEIARQYGAQVTAVDIAPVMLERADANVTAAAVNDRVKVAP
                     GDILDLDYDDHSFDVVIAEAVTMFVDRQRAANELARVTKPGGHVLATEFFWRQPPTPE
                     AKEIFLGQV"
     gene            621..1304
                     /locus_tag="VF954_00420"
     CDS             621..1304
                     /locus_tag="VF954_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9529567.1"
                     /translation="MGDRAAKDALFEGFAEVAKAMASGRRAEVVDVLAQCERSVDEIA
                     EEIGQSVANTSHHLRAMARAGLVTTRRDGTRIFYRLASERVAELWAALRDVAAEHVAG
                     LEKLAAAYLGDRDGIDTIDRKELAQRIKRGEVVLLDVRPEAEYTAGHIAGARSVPVSE
                     LRRHLKALPADAEVVAYCRGPYCVYADDAVRELTRKGYRARRLLDGFPEWKRAGLPVA
                     AGTGGSIDG"
     gene            1297..1902
                     /locus_tag="VF954_00425"
     CDS             1297..1902
                     /locus_tag="VF954_00425"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9529568.1"
                     /translation="MAEDLLVDPDVLREQVRHKYREVATHPNRMFHFHTGRPLAARLG
                     YDPGAVDPLTDRAVESFAGVGNPLSLRGLQPGEQVVDVGSGAGFDTFIAAGQVGDTGR
                     VVGVDMTPEMLKKSRATAEALGYAHVEFRQGLAEQLPVEDGWADAVISNGVINLCADK
                     KAVFTEIYRVLRPGGWLQFADIANGRPVPPEALRDIDLWTG"
     gene            1939..2070
                     /locus_tag="VF954_00430"
     CDS             1939..2070
                     /locus_tag="VF954_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529569.1"
                     /translation="MLEDTGFTGVRTGPPADTFAGATGEDKARAYEVYGYAFLARKP"
     gene            2067..2702
                     /locus_tag="VF954_00435"
     CDS             2067..2702
                     /locus_tag="VF954_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9529570.1"
                     /translation="MNTTTQHKEERPWQASSTTPSRADGGDARSVLDCGDPDKLAEFW
                     SAAVGYVPLGGAGNYVLLLDEAGQQPKLLQQRVTEPKAGKNRMHFDIVPIGDANARIE
                     RDVIGAVWGANGYTTVAQANELSRHLDLGPASRLLDLGTGCGWPGLYLAKQSGCSMVG
                     TDMPVEGLAIAARRAGAEGMDQRVSLVAAAGAAQPFRPASFDAIVLTDVLC"
     gene            complement(3113..3535)
                     /locus_tag="VF954_00440"
     CDS             complement(3113..3535)
                     /locus_tag="VF954_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529571.1"
                     /translation="MSATQERNSESVARWSVGGVFLEALANRDYPRMATTLAPAVKFR
                     AMLPPGPMDWEGPDTVSGVFSSWFGDAADFEVVDATVGEVGGRLHLSWRLRVRPAPFG
                     IGDGWHVIEQQAYADAGDTIDSLDLLCSGFRAETATSA"
     gene            3719..>3900
                     /locus_tag="VF954_00445"
     CDS             3719..>3900
                     /locus_tag="VF954_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529572.1"
                     /translation="MEHVLRSWVIAARLGDHVGLAIDDRSALFYVATLAWVGCVADTP
                     ELATWFGDDIVFRRDSR"
BASE COUNT          592 a         1356 c         1342 g          610 t
ORIGIN      
        1 cacttgaccg aggaagatct ccttcgcctc cggcgtgggt ggttgccgcc agaagaactc
       61 ggttgcgaga acgtgtccgc ccggcttggt cacgcgggca agctcgttgg cggcgcgttg
      121 gcggtcgacg aacatcgtca ccgcctcggc gatcaccacg tcgaagctgt ggtcgtcgta
      181 gtcgaggtcg aggatgtcgc ccggcgcgac cttgacgcga tcgttgaccg cggcggcggt
      241 gacgttggcg tcggcccgct ccagcatcac cggggcgatg tcgaccgcgg tgacctgcgc
      301 accgtactgg cgggcgatct cgaccgcggt ggtggccact ccgcacccga catcgaggac
      361 ccgactgtca gcggtgatgc cggcccggtc gagcagtgcg cgggttgatg cgcggccgcc
      421 ggggtggatg acccgcttgc cgatcacggc catgaacttg tacgggtcga gttcgccgat
      481 ctcggccgcg gccagcttgg tcgccatcgc gcacctcccg acttcctccg gtactcgcat
      541 ggtgctctcg ctctgtcccg attgtcaagt aatcgtctga gcacttgaca ggtcgggtcg
      601 acgggcgcac catgagccgc gtgggagacc gggccgccaa ggacgcgctg ttcgaaggct
      661 tcgccgaggt ggccaaggcg atggccagcg gtcgccgggc cgaggtcgtc gatgttctgg
      721 cccagtgcga gcggtcggtg gacgagatcg ccgaggagat cggccaatcg gtggccaaca
      781 cctcccacca cctgcgggcc atggcccgcg ccgggctggt caccacccgg agggacggga
      841 cccgcatctt ctaccggctg gccagcgaac gggtggccga gctctgggcc gcgctgcggg
      901 acgtggccgc cgagcacgtc gccggtctcg agaagttggc ggctgcctac ctcggcgacc
      961 gggacggaat cgacaccatc gaccggaagg aactggcaca gcgaatcaag cggggcgagg
     1021 tcgtcctgct cgacgtccgg cccgaggccg agtacaccgc cggccacatc gccggcgccc
     1081 gatcggtccc ggtcagcgag ctccgccgtc acctgaaggc gctgcccgcc gacgcagaag
     1141 tcgtcgccta ctgccgcggc ccgtactgcg tctacgccga tgacgccgtt cgggagctga
     1201 cccgcaaggg gtatcgggcc cggcggctgc tcgacggctt ccctgaatgg aagcgggctg
     1261 gcctgccggt ggccgccggc acgggaggga gcatcgatgg ctgaggatct cctcgtcgac
     1321 cctgacgtac tccgggagca ggtgcggcac aagtaccgcg aggtcgccac ccacccgaac
     1381 cggatgttcc acttccacac cgggcggccg ctggcggcgc gcctgggcta cgacccgggc
     1441 gccgtcgacc cgctcaccga ccgggccgtg gaatcgttcg ccggcgtcgg caacccgttg
     1501 tcgctgcggg gcctgcagcc gggcgagcag gtcgtcgacg tcggctccgg tgcagggttc
     1561 gacaccttca tcgccgccgg ccaggtcggc gacaccgggc gcgtcgtcgg tgtcgacatg
     1621 accccggaga tgctgaagaa gtcccgggcc accgccgagg cgctcggcta tgcccacgtc
     1681 gagttccgcc aaggcctggc cgagcagctc ccggtcgaag acggctgggc cgacgccgtc
     1741 atcagcaacg gggtcatcaa cctgtgtgcc gacaagaagg cggtgttcac cgagatctac
     1801 cgggtgctgc gcccgggcgg gtggctgcaa ttcgccgaca tcgccaacgg ccggcccgtc
     1861 ccgcccgagg cgttgcggga catcgacttg tggaccggct gaatcgccgg cgggctgccc
     1921 cgtgcgggct ggcagaagat gctcgaagac accggcttca ccggcgtgag gaccgggccg
     1981 cccgccgaca cgttcgccgg cgccaccgga gaggacaagg cccgggcgta tgaggtgtac
     2041 ggctacgcct tcctcgcccg caaaccctga acaccaccac ccagcacaag gaggaacgac
     2101 catggcaggc gtcgagcact acgccaagcc gagctgatgg cggcgacgct cggtctgttc
     2161 tcgattgcgg ggacccggac aagctcgccg agttctggtc ggccgccgtc ggttacgtgc
     2221 ccctcggcgg tgccggcaac tacgtgcttc tcctcgatga ggctggccag cagccgaagc
     2281 tgctgcagca acgggtcacc gaacccaagg ccggcaagaa ccgcatgcac ttcgacatcg
     2341 tgccgatcgg cgacgccaac gcccggatcg aacgtgacgt catcggggca gtgtggggcg
     2401 ccaacggcta caccaccgtt gctcaggcga acgagctctc tcgccacctg gacctggggc
     2461 cggcctcccg gttgctcgat ctgggcaccg gctgcgggtg gcccggcctg tacctggcca
     2521 agcaatccgg gtgctcgatg gtgggcaccg acatgccggt ggaaggcctc gccatcgcgg
     2581 cgcgccgagc aggcgccgaa ggcatggacc agcgcgtctc attggtcgcc gcagccggcg
     2641 ccgcgcagcc cttccggccc gcttcgttcg acgccatcgt gctcaccgac gtgctgtgtt
     2701 gactgtggcg agagctcgcc gtgctgtggg cgctcaaaca gctgctgcgt ccgggcgggc
     2761 tcattgccta cacgacgatc ttcgtcacgc ccgggctcga cccgctccgc cgtcgtcggg
     2821 cccgccgatc tggtcctcgg gcggtcgggt cgcgcagcga ccaacgccga ctcctggtct
     2881 cagctggttt caccgacatc gaagtggtcg atctcacacc ggagtgggtc cgcaccgcgc
     2941 ggggctggtt ggcgggatgg acagccaacg aagcagagct caccagactc gagtcacccg
     3001 tcgccgtcgc tcaacgccaa acagagcgcc aaacgcagct gcgtgctctc gacgacggcc
     3061 tcctgcgccg ggcccttttc tcggcggcgc gctctcgatg acgcgacccg acctatgcgc
     3121 tggttgctgt ctcggctcgg aagccggagc acaacaggtc gagcgagtcg atggtgtcgc
     3181 cggcatcggc gtacgcctgc tgctcgatca catgccagcc gtcgccgatg ccgaacggcg
     3241 ccggtcgcac ccgcagccgc caagacaggt gcaggcggcc gcccacctcg ccaaccgtgg
     3301 cgtcgacgac ctcgaagtcg gcggcgtcgc cgaaccagga gctgaagacg ccgctaaccg
     3361 tgtcgggtcc ttcccaatcc atcgggcctg gcggcagcat ggcccggaac ttcacggccg
     3421 gtgccagggt ggtcgccata cgcgggtagt cgcggttggc gagtgcttcg aggaacactc
     3481 ccccgaccga ccagcgggcc accgactcgc tgttacgctc ctgtgttgct gacatggcgt
     3541 cctcccattc ggtctctgtg cagtcgagag tacggacgcg ttctggcggc gtcgtccggg
     3601 gttcacccca caaatcaaag gcggtgcaat ggatgtgact gatcgcgact cgggggtcgc
     3661 gctggccgag ctcgtcgggg cgttctcgct ctcaaccgac ttgggactag gtcagcccat
     3721 ggagcacgtg ctgcgatcgt gggtcatcgc cgcccgcctg ggcgaccacg tcggactcgc
     3781 gatcgacgat cgcagcgctc tgttctacgt ggccaccctg gcgtgggttg gctgtgtcgc
     3841 cgacacgccc gagctggcga cctggttcgg cgacgacatc gtcttccggc gcgacagccg
//
LOCUS       DASWGG010000026         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_995922, whole genome shotgun sequence.
ACCESSION   DASWGG010000026 DASWGG010000000
VERSION     DASWGG010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995922"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VF954_00450"
     CDS             complement(<1..1179)
                     /locus_tag="VF954_00450"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529573.1"
                     /translation="MAGIDRGFDYLVPDGAGPIEVGTIVRVPLQGRRVAGWVEQLDPA
                     PAPGVHLRPLLSVSSVGPSPELVALAGWAARRWAGRLAVFLRAASPPVNVRQAPTGDA
                     TPPRTGHARSVIRLAPAGDWWPVVRSVAGGMGKGQLLLLCPSVAAAASVAGRLRRDGQ
                     AVALLPREWPQAAAGASFVVGVRAAAWGPAPLLGAVMVLDAHDQAFQEERAPTWRAWD
                     VAAERARRADAPCVLVSPCPSLEQLHWGELHVGARQDERAGWAPLEVIDRRTEDPRAG
                     WLAARLVDVIRDPEATPDRPVVCVVNRKGRARLLACGSCGSLATCERCGAAVGQPAAG
                     TDTGPPALACGRCGDTRPRVCLACGSQRFKNLRPGVTRLREEVEALANRPVAEVTGEA
                     G"
     gene            complement(1206..>2324)
                     /gene="metK"
                     /locus_tag="VF954_00455"
     CDS             complement(1206..>2324)
                     /gene="metK"
                     /locus_tag="VF954_00455"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX9529574.1"
                     /translation="TVLDAILDQDPHGRVACETMLTTGLVVVAGEITTKGYVDIATLA
                     RQAICDIGYDREAHGFDGNSCGVVVSIDEQSPDIDQGVTAALEMRTGSGGEDLLNAQG
                     AGDQGMMFGYACDETPDLMPLPIWLAHRLAERLAQVRRAGILTYLRPDGKTQVTVDYE
                     DGRPKALRTVLISTQHGPDIDLEHQLKPDLVTHVIEPTVPTELGSGYDVLANPTGRFE
                     LGGPRADAGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSGAYAARWVAKHVVAAG
                     AARRCEIQVAYAIGVAHPVSIMVDTFGTESVDPATISEMVRDVFDLRPAAIIRDLDLR
                     RPIYRRTAAYGHFGRTEKEFSWEATSRLDDVKSALGLR"
BASE COUNT          292 a          873 c          868 g          291 t
ORIGIN      
        1 gcccgcctcg ccggttacct cggcgacggg ccggttggcc agggcctcga cttcttcgcg
       61 gaggcgggtg acaccggggc ggaggttctt gaaccgctgg gagccgcagg ccaggcacac
      121 ccggggccga gtgtcgccgc agcggccgca ggcgagcgcc ggtggtccgg tgtccgtgcc
      181 ggccgcgggc tgcccgacgg ccgcgccgca gcgctcgcag gtggcgaggg agccgcacga
      241 cccgcacgcc agcagccggg cccggccctt gcggttgacc acgcagacca cgggccgatc
      301 aggggtcgcc tccgggtccc ggatcacgtc caccaggcgc gccgccagcc atccggcgcg
      361 cgggtcctcc gtgcgccggt cgatgacctc cagcggggcc caccccgccc gctcgtcctg
      421 ccgggcgccg acgtgcagct ctccccagtg cagctgctcg aggctcgggc acggcgacac
      481 caggacgcag ggtgcgtcgg cccgccgggc gcgctcggcc gcgacgtccc atgctcgcca
      541 cgtcggggcc cgctcctcct ggaaggcctg gtcgtgggcg tcgagcacca tcaccgcgcc
      601 gaggagggga gcggggcccc aggccgcggc gcgcacaccc accacgaagg acgccccggc
      661 cgcggcctgg ggccactctc gcggcagcag cgccaccgcc tggccgtcgc gtcgcaggcg
      721 gccggccaca gacgccgccg cggccaccga ggggcacagc aggaggagct gccctttccc
      781 catcccgccg gccacggacc gcacgaccgg ccaccagtcc ccggccgggg ccaggcggat
      841 gaccgaccgg gcgtggcccg tcctcggcgg cgtggcgtcg ccggtcgggg cctgccggac
      901 gttgaccggc ggcgaggcgg cccgcaggaa cacagccagg cgcccggccc atcgacgggc
      961 agcccagccg gcgagggcga ccagctccgg gctcggcccc acgctcgaca ccgatagcag
     1021 agggcggaga tgcacgcccg gtgccggcgc gggatccagc tgctcgaccc agccggccac
     1081 ccgccgcccc tggagcggga cgcgcacgat ggtgcccacc tcgatcgggc ccgctccgtc
     1141 gggcaccagg tagtcgaagc ccctgtcgat cccggccacg tcgggccgga cccggacgcg
     1201 gcgggtcagc gcaatccgag ggcgctcttc acgtcgtcca ggcgggaggt cgcctcccag
     1261 ctgaactcct tctcggtacg gccgaagtgg ccgtaagccg ccgtgcggcg gtagatcggc
     1321 cggcgcaggt cgaggtcgcg gatgatcgcc gccggccgca ggtcgaagac gtctcgcacc
     1381 atctcggaga tggtggcggg gtcgaccgac tcggttccga aggtgtccac catgatcgac
     1441 accgggtggg cgaccccgat ggcataggcc acctggatct cgcagcgccg ggcggccccg
     1501 gccgccacca cgtgcttggc cacccaccgg gcggcgtagg cgccggagcg gtcgaccttg
     1561 ctggggtcct tgccggagaa ggccccgccg ccgtggcggg cggcgccgcc gtaggtgtcg
     1621 acgatgatct tgcgccccgt gaggccggcg tcggcgcggg gcccgcccag ctcgaagcgg
     1681 cccgtcgggt tggccagcac gtcgtagccc gagcccagct cggttggcac ggtcggctcg
     1741 atgacgtggg tgaccagatc gggcttcagc tgatgctcga ggtcgatgtc ggggccgtgc
     1801 tgggtgctga tgagcaccgt ccgcagcgcc ttcgggcggc cgtcctcgta gtcgaccgtc
     1861 acctgggtct tgccgtcggg gcgcaggtag gtgaggatgc cggcccggcg gacctgggcc
     1921 aggcgctccg ccagtcggtg ggccagccag atcggcagcg gcatgaggtc cggcgtctcg
     1981 tcgcacgcgt agccgaacat catcccctgg tcgccggcgc cctgggcgtt gagcaagtcc
     2041 tccccgccgg agccggtccg catctcgagc gccgccgtga cgccctggtc tatgtcgggt
     2101 gactgctcgt cgatggagac gaccacgccg caggagttgc cgtcgaagcc gtgggcctcc
     2161 cggtcgtagc cgatgtcgca gatcgcctgg cgggccaggg tggcgatgtc cacgtagccc
     2221 ttggtcgtga tctcccccgc caccaccacc agcccggtgg tcagcatcgt ctcgcaggcc
     2281 acccggccgt gggggtcctg gtcgaggatg gcgtcgagga ccgt
//
LOCUS       DASWGG010000027         1830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_996656, whole genome shotgun sequence.
ACCESSION   DASWGG010000027 DASWGG010000000
VERSION     DASWGG010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996656"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VF954_00460"
     CDS             complement(<1..745)
                     /locus_tag="VF954_00460"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEX9529575.1"
                     /translation="MVLAAALGFAFLPGHGAAAHALVASSDPAAGATLASAPSQIVLT
                     FTEQPDLKRSLIQVLDTSGKKLAGGAPEGVAGQPTTVARIPVTTTLPKGVYTVSWKSI
                     SAVDGHLATGAFAFGVGEAPRPGGPSTAVKSPGASEVGVAGRWFYLGGLMGLLGIAFV
                     QLVVLRGSARPRRLGPALWVSWVAAAGGLLAMTQAQASGAGLGIGDLLSSSIGHALLV
                     RGIPLLVAGLGLVVGRSGGRIGGGLLVVAT"
     gene            complement(791..1492)
                     /locus_tag="VF954_00465"
     CDS             complement(791..1492)
                     /locus_tag="VF954_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529576.1"
                     /translation="MRGFRFTLGAVALGLAGLLIAAPAFAHTEKDAGPYKLLFGWRTE
                     PAYTGEQNAVQLFVHDTSGKAIDSLGSKGLTVVVSTGTGATAKSSSPMALNSAFDPDS
                     GLGTHGEFDAPILPTAPGTYTFHITGDINGTAVNVSAASSDPTFANVTDPTAIEFPVQ
                     TQTVPQLASAITTLQDRLAGAQSAAANAQSAANAAKSSATTSKTLAIVAVVVAFVLGL
                     IALVAGRRKKARSAG"
     gene            complement(1639..>1830)
                     /locus_tag="VF954_00470"
     CDS             complement(1639..>1830)
                     /locus_tag="VF954_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529577.1"
                     /translation="VGAAGGLALVGLDRAGERAGRAVGSRRRTTRRRTQAQPPAPDSP
                     TPKLLPATSCDIRGPPLPA"
BASE COUNT          257 a          623 c          710 g          240 t
ORIGIN      
        1 gggtggcgac gacgaggagc cccccgccga tccggccgcc gctccggcca acgaccagcc
       61 cgagccctgc caccagcagc gggatgcccc ggaccaggag ggcatgtccg atcgagctcg
      121 acagcaggtc gccgatgccg aggcccgctc cggacgcctg tgcctgggtc atggccagta
      181 acccgccggc ggccgcgacc cacgacaccc acagggcggg gccgagccgc cgcgggcgag
      241 ccgagccccg gagcaccacc agctggacga aggcgatgcc gagcagcccc atgagcccgc
      301 cgaggtagaa ccagcgcccg gccacgccca cctccgaggc acccggcgac ttgaccgccg
      361 tgctggggcc gccggggcgg ggcgcctcgc ccacgccgaa cgcgaacgcc cccgtcgcca
      421 ggtggccgtc caccgccgag atcgacttcc acgagacggt gtagacgcct ttgggcaggg
      481 tcgtcgtgac cgggatcctg gccaccgtcg tcggttggcc cgccaccccc tcgggggcgc
      541 cgccggcgag cttcttcccc gaggtgtcga ggacctggat cagcgatcgc ttgaggtcgg
      601 gctgctcggt gaaggtgagg acgatctggg agggtgccga tgccagcgtc gcgcccgccg
      661 cggggtccga cgaagcgacc agtgcgtgcg ccgccgcgcc gtgccccggc aggaaggcga
      721 accccagcgc ggcggcaagc accacgcccc agaggagccg gacgggacct ccccggcggg
      781 agtgttgcat ctagccggcg cttcgtgcct tcttgcggcg gccggcgacc agggcgatca
      841 ggccgagcac gaaagcgacg acgacggcca cgatcgccag cgtcttggac gtcgtggcgg
      901 agctcttggc ggcattggcc gccgactggg cgtttgcggc ggccgactgg gcgccggcga
      961 gccggtcctg cagggtcgtg atggcggagg ccaactgcgg aacggtctgg gtctgcacgg
     1021 ggaactcgat ggcggtggga tcggtgacgt tggcgaaggt cgggtccgac gacgccgccg
     1081 acacgttcac cgcagtcccg ttgatgtcgc cggtgatgtg gaacgtgtac gtgcccggag
     1141 cggtcggcag gatcggtgcg tcgaactcac cgtgggtgcc gaggccgctg tccgggtcga
     1201 acgcgctgtt cagggccatg gggctgctcg acttggcggt cgccccggtg ccggtgctga
     1261 cgacgacggt caggcccttg gagcccaggg agtcgatcgc cttcccgctg gtgtcgtgga
     1321 cgaagagttg gacggcattc tgctccccgg tgtaggcggg ctcggtccgc caaccgaaga
     1381 gcagcttgta ggggccggcg tccttctcgg tgtgcgcgaa ggcgggcgcg gcgatgagca
     1441 ggccggcaag ccccagggcg acggcgccca gggtgaatcg aaaacctcgc acgggggtgt
     1501 tcctttcttc gggatgtcag agggcggcgg tcccttggga gtaggcaacg gggttacgca
     1561 acggcccaag ggggtgcacg tggtgcgcag ccgctgcgag gtattcgcag aggagcgccc
     1621 tctagcctgg tcggagggtc aggccggcag cgggggaccc cggatgtcgc aggatgtggc
     1681 ggggaggagc ttcggcgtcg gtgagtcggg tgcgggcggt tgcgcctgcg tgcgccgccg
     1741 ggtcgtgcga cggcgggacc cgactgcccg gcccgcccgc tctccggccc ggtccagccc
     1801 gacgagcgcc aggccgccgg cggccccgac
//
LOCUS       DASWGG010000028         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2931718, whole genome shotgun sequence.
ACCESSION   DASWGG010000028 DASWGG010000000
VERSION     DASWGG010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2931718"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..459
                     /locus_tag="VF954_00475"
     CDS             76..459
                     /locus_tag="VF954_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012231502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX9529578.1"
                     /translation="MGLRPGLSAQIELTVGQADTALDLRSGSVDVLGTPRLIALCEQA
                     TCAATAGMLGPAETTVGMRVQFDHLAPTPVGHTVTAEAVLEKIEGRRLTFTVSASDHG
                     GLVGAGRITRVVVDVERFLKKSAGA"
     gene            complement(475..>2184)
                     /locus_tag="VF954_00480"
     CDS             complement(475..>2184)
                     /locus_tag="VF954_00480"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HEX9529579.1"
                     /translation="AGHSPQFEAPEAWWQALSGFLDGVAGRAVAPPVRRSGHGGELAV
                     AALRERGVEVIFTLSGGHLFPIYDGCVRQGVRIVDVRHEQTATFAAEGVAKLTRGLGV
                     AALTAGPGVTNAISAITTARMNGSPLLVLGGRASQRRWGGGALQELDHLPVVSSLAKL
                     AATATTTRGCGFLVAQAVDAAMAPHRGPVFLDFPMDVLFGAGEVELEAPAPPAGREPD
                     PGEVEKVAALVAGASRPALVAGGDVWWGGAEEALARCVEALRVPTFANGLGRGCLPAD
                     HELAFSRARPALKEADVVVVVGTPLDFRLSFGRFGAASVVHIVDHPDQRARHVSVAAS
                     PAGDLAAILDGMAAHAGPRADREPWIASLRDRERAARQAERAELESGAWPIHPARIYG
                     ELRPRLDRDAVIVCDGGDFVSYAGKLIDSYRPGCWLDPGPYGCLGTGMGYSIAARTVH
                     PGRQVVAVLGDGAFGFAGMDVDSLVRHRLPVVMVVGNNGVWGLEKHPMQALYGYSVAT
                     DLQPGCRYDEVVRALGGAGETVEDPAQIGPALDRALAAGVPYLVNVLTDPAVAYPRSS
                     NLG"
BASE COUNT          313 a          799 c          830 g          242 t
ORIGIN      
        1 gcgtcgaagt ggtgaccggc cgccaccccc gccggcaggg cggcggcggc ctccacaggg
       61 cgtacgctca agagagtggg actacgcccc gggctgtccg cccagatcga gctgaccgtg
      121 gggcaggccg acaccgccct cgacctcagg tcgggttcgg tcgacgtgct cggcaccccg
      181 aggctgatcg ccctctgcga gcaggccacc tgcgcggcca cggcggggat gttgggcccg
      241 gcggagacga cggtggggat gcgggtccag ttcgaccacc tggcccccac gccggtcggg
      301 cacacggtga cggccgaggc cgtgctggag aagatcgaag gccggcgcct gaccttcacc
      361 gtctcggcca gcgaccacgg cgggctggtc ggggcgggca ggatcacccg ggtcgtcgtg
      421 gacgtcgagc ggttcctaaa gaagtcggcg ggggcgtgac cttcaaccgt gccttcaacc
      481 gaggttggac gacctcgggt acgcgacggc gggatcggtg aggacgttga ccagataggg
      541 cacgcccgcg gccagggccc ggtccagggc cgggccgatc tgggccgggt cctccaccgt
      601 ctcaccagcg cctccgagcg cccggaccac ctcgtcgtag cggcagccgg gctggaggtc
      661 ggtggccacg gagtatccgt acagcgcctg catcgggtgc ttctccagcc cccagacgcc
      721 gttgttgccg acgaccatga cgacggggag ccggtggcgc acgagggagt ccacgtccat
      781 gccggcgaag ccgaaggccc cgtcgccgag gacggcgacc acctggcggc cggggtgcac
      841 cgtccgggcg gcgatggagt accccatgcc ggtgccaagg caaccgtagg gcccaggatc
      901 gagccagcaa ccgggccggt aggagtcgat cagcttgccg gcataggaca cgaagtcccc
      961 accgtcgcag acgatcaccg cgtcccgatc gaggcggggg cgcagctcgc cgtagatccg
     1021 ggccgggtgg atgggccagg ccccggactc gagctcggcc cgctccgcct ggcgggcggc
     1081 cctctcccgg tcgcgtagcg aggcgatcca gggctcgcgg tcggcccgcg ggccggcgtg
     1141 ggccgccatg ccgtcgagga tggcggccag gtcccccgcc ggcgaggccg ccaccgacac
     1201 gtgccgggcc cgctggtccg ggtggtcgac gatgtggacc acgctggccg cgccgaagcg
     1261 gccgaaggac agccggaagt cgagcggggt gccgaccacc acgaccacgt cggcttcctt
     1321 caaggccggg cgggcccggg agaaggcgag ctcgtggtcg gccggtaggc agccccggcc
     1381 cagcccgttg gcgaaggtcg ggacccggag cgcctccaca caccgggcga gagcctcctc
     1441 ggctcctccc caccacacgt ccccgccggc cacgagcgcg gggcgcgacg caccggcgac
     1501 gagggcggcc accttctcga cctcgccggg atcgggctcc cggccggccg gcggagcggg
     1561 agcctcgagc tcgacctcgc cggcgccgaa cagcacgtcc atcgggaaat ccaggaagac
     1621 cgggccccgg tgcggcgcca tggcggcgtc cacggcctgg gcgaccagga agccacagcc
     1681 ccgggtggtc gtggccgtcg cggccagctt ggccagcgac gacacgacgg gcaggtggtc
     1741 gagctcctgg agggccccac caccccaccg gcgctgcgag gcccggccgc ccagcacgag
     1801 cagcggggag ccgttcatgc gggcggtggt gatggcgctg atggcgttgg tcacgcccgg
     1861 gccggccgtg agagcggcca cgccgaggcc ccgggtcagc ttggcgacgc cctcggccgc
     1921 gaaggtggcc gtctgctcgt ggcgcacgtc gacgatccgc accccctgtc tcacgcagcc
     1981 gtcgtagatg gggaacaggt ggccgcccga gagggtgaag atgacctcca cgccccgctc
     2041 ccgcagggcg gccaccgcca gctcgccgcc gtggcccgac cggcgcacgg gcggggcgac
     2101 ggcccggccg gcgacgccgt cgaggaagcc cgacagcgcc tgccaccacg cctcgggcgc
     2161 ctcgaactga ggcgagtggc cggc
//
LOCUS       DASWGG010000029         2936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_998035, whole genome shotgun sequence.
ACCESSION   DASWGG010000029 DASWGG010000000
VERSION     DASWGG010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998035"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VF954_00485"
     CDS             <1..308
                     /locus_tag="VF954_00485"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529580.1"
                     /translation="RSVVAMNAQWLGPWNLGPKAHPNDGLLDISDARLSCADLPKVRA
                     RARLGAHLPHPRNRLQRTGAAQVDLERPLPVFVDGVCVGRGRQLAFRALPDALTVFV"
     gene            390..2024
                     /locus_tag="VF954_00490"
     CDS             390..2024
                     /locus_tag="VF954_00490"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX9529581.1"
                     /translation="MPPYALERFTPPEEDILRRYFTNLDRPVFALVNLPEVVKGALFA
                     RYSRSAKSLRRLFLDEFVGDLDISGDVSVDATVGLRRAEELYDRVFMEYGDDSVAQLG
                     GVHLACEQASNLLTKILERGRLMSYLEQSTRYVAYDTRLDNGRYRYYRDPAILDSSLG
                     ARYVGEMDRMFDTYAELMPRLTAWFSERVGKAPGDSDFVHRQAIRAKAFDALRGLLPA
                     ASLSNVGIYGTGQGYEALLLRMRAHPLPEARAFAAMMLEELRKVIPSFLKRVDLPDRG
                     GAWTAYLERNRAAVEAVAARLLEGEAPAPRPAVTLVDFDPDGEDKVVAAALYPHARLP
                     EDQVLARVRRLAPDEKAAVLRAYVGERSNRRHKPGRAFERVSYRFDVVSDYGAFRDLQ
                     RHRMLTIEWQDLTPEHGYVLPEAVTAAGGGEAFAESMDRSAELHAALRERFPARAAYS
                     VALAYRIRYVMQLSAREAMHVIELRSSPQGHPEYRRVAQEMHRLIAEAAGHRLIADAM
                     GFVDHADYRLDHLERLESERRADARRSQTRPTTCST"
     gene            2078..2893
                     /locus_tag="VF954_00495"
     CDS             2078..2893
                     /locus_tag="VF954_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX9529582.1"
                     /translation="MLLSGMFNHAGGPKDRWFMPSRRLRVVTTSVGVVVSLAVCAQGG
                     GAAYTVRAGDTLTAVARSLKTSVSALATTNHLADPNRIYVGQVLQLPPGSGPGADPPA
                     HPGAPAPQPGPIVDGPVAVYPPGSAPLVTVSPARVLAGHPDRRALGPMFDRAAHQAHV
                     PANLLKAIAWQESGWQNNVFSVVRAQGIGQLMPGTVTFVNALTGQHLDPSKPADNITL
                     EAALLAFLLYNARGDQPTAIAGYYQGLASVQQRGLYDDTKAYVANVVALRAMF"
BASE COUNT          393 a         1150 c          975 g          418 t
ORIGIN      
        1 gccgctcggt ggtggccatg aacgcccagt ggctcggccc gtggaacctc gggcccaagg
       61 cccatcccaa cgacggcctc ctcgacatct ccgacgcccg cctcagctgc gccgacctgc
      121 ccaaggtccg ggcccgggcc cggctcggcg cccacctgcc ccacccccgg aaccgtctcc
      181 agcgcaccgg ggcggcccag gtcgacctcg aacggccgct gccggtgttc gtggacggcg
      241 tctgcgtggg caggggccgc cagctcgcct tccgggccct ccccgacgcc ctgaccgtct
      301 tcgtttgagc tttggccacg tagggcgctc tgtccgcgcc cagcgcggtc caccacggca
      361 tcggggtcgg ggccgtaacc tccccccttg tgccgccgta cgccctcgag cgcttcaccc
      421 cgccggagga agacatcctc cggcgctact tcaccaacct ggaccggccc gtcttcgccc
      481 tcgtcaacct gcccgaggtg gtcaaggggg ctctgttcgc ccgctactcg cggtcggcca
      541 agagcttgcg gcggctgttc ctggacgagt tcgtcggcga cctcgacatc agcggcgacg
      601 tgagcgtcga cgccaccgtg ggcctgcgcc gggccgagga gctctacgac cgggtcttca
      661 tggagtacgg cgacgactcc gtggcccagc tcggcggggt ccacctggcc tgcgagcagg
      721 cgtccaacct gctgaccaag atcctggagc ggggccggct catgtcctac ctcgagcagt
      781 cgacccgcta cgtcgcctac gacacccgcc tcgacaacgg ccgctaccgc tactaccggg
      841 acccggccat cctcgactcc tccctggggg cgcggtacgt gggggagatg gaccggatgt
      901 tcgacaccta cgccgagctc atgcccaggc tgaccgcgtg gttctcggag cgcgtgggca
      961 aggccccggg cgactccgac ttcgtgcacc gccaggccat ccgggccaag gccttcgacg
     1021 ccctgcgggg gctgctgccc gcggcctcgc tgtccaacgt cggcatctac ggcacgggcc
     1081 agggctacga ggccctgctc ctgcggatgc gggcccaccc gctgccggag gcccgggcct
     1141 tcgcggccat gatgctcgag gagctgcgca aggtcatccc gtcgttcctc aagcgcgtcg
     1201 acctgcccga ccggggaggg gcctggacgg cctacctgga gcgcaatcgc gccgccgtgg
     1261 aggcggtcgc ggcccggctg ctggaggggg aggcgcccgc gccgcggccc gccgtcaccc
     1321 tggtggactt cgaccccgac ggcgaggaca aggtcgtcgc cgccgccctc tacccccacg
     1381 cccggctccc ggaggaccag gtcctggccc gcgtacggcg cctggccccg gacgagaagg
     1441 ccgccgtgct gcgcgcctac gtcggggagc ggtccaaccg gcgccacaag ccgggccggg
     1501 ccttcgagcg ggtgtcgtac cgcttcgacg tcgtctcgga ctacggggcc ttccgggacc
     1561 tccagcgcca ccggatgctc accatcgaat ggcaggacct cacgcccgag cacggctacg
     1621 tgctgccgga ggcggtcacg gccgccggcg gcggggaggc cttcgccgag tcgatggacc
     1681 gttcggccga gctgcatgcc gccctccggg agcgcttccc ggcccgggcc gcctactcgg
     1741 tggcgctggc ctacaggatc cgttacgtca tgcagctgtc cgcccgcgag gccatgcacg
     1801 tcatcgagct gcgcagctcg ccccagggcc accccgagta ccggcgggtc gcccaggaga
     1861 tgcaccgcct catcgccgag gccgccgggc accgcctcat cgcggacgcc atgggcttcg
     1921 tcgaccacgc cgactaccgc ctcgaccatc ttgagcggct ggaatcggag cggcgggcgg
     1981 acgcccgccg gtcgcagacc cggcccacaa cctgctccac ctgaggacgt cgatctcgcc
     2041 ctcaacctcg tggctggtga ggtcgatgtt gcacctgatg ctgttgagcg gtatgttcaa
     2101 tcacgcgggc ggtcccaagg atcggtggtt catgccctcc cgtcggctgc gagtggtcac
     2161 cacgagcgtc ggcgtcgtcg tgtcgctcgc ggtctgcgcc cagggcgggg gggcggccta
     2221 caccgtgcgc gccggcgaca cgctgactgc cgtggcccgg tcgctcaaga caagtgtcag
     2281 cgccctggcc accaccaacc acctcgccga tcccaaccgc atctacgtcg gccaggtcct
     2341 gcagctcccg cccggctcgg gcccgggcgc ggatcccccc gcccacccag gcgcccctgc
     2401 gccccaaccg gggccgatcg tcgacggccc cgtggccgtc tacccgccag ggtcggcgcc
     2461 gctggtgacg gtcagcccgg ctcgcgtcct agccggccac ccggaccgcc gggcgttggg
     2521 gcccatgttc gaccgggccg cccaccaggc gcatgtaccc gccaacctgc tgaaggccat
     2581 cgcctggcag gagtccggat ggcagaacaa cgtgttctcg gtggtccgcg cccagggcat
     2641 cggccagctg atgccgggca cggtgacgtt cgtcaacgcc ctgaccggcc agcacctcga
     2701 tccctccaag ccggctgaca acatcaccct ggaagccgcg ctgctggcct tcctgctgta
     2761 caacgcccga ggcgaccagc ccaccgccat cgccggctac tatcagggac tggcgtcggt
     2821 ccaacagcgc gggctctacg acgacaccaa ggcctacgtg gccaacgtgg tggccctgcg
     2881 cgcgatgttc taggcccggc cggggcccca accccggcct cctccgcacg gcgggg
//
LOCUS       DASWGG010000030         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_998748, whole genome shotgun sequence.
ACCESSION   DASWGG010000030 DASWGG010000000
VERSION     DASWGG010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998748"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VF954_00500"
     CDS             <1..536
                     /locus_tag="VF954_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529583.1"
                     /translation="AASTPAAPATLERDRRVRRLVRRARALALAAFTALLAAASGLGV
                     EIQVRSHAHIPSLHDQAFVTAARSDCALALDPIRMSRPPAGTAENAARIDGLATALDG
                     VAAQIRTLPVEARDQAAVTAWLGAWQTYSSVAHRYADALRSGAKGPGRVAQMASAARS
                     EIDGFAEVNHLHACAVG"
     gene            541..1170
                     /locus_tag="VF954_00505"
     CDS             541..1170
                     /locus_tag="VF954_00505"
                     /inference="COORDINATES: protein motif:HMM:NF017697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HEX9529584.1"
                     /translation="MSFAELLAHPDVVEVVELRSSVGFLAFHGGSLEKATDVVAAGAA
                     RRSGASLYAVLQPPGLRWHIPSHTVDPAHSAALASFLDQVEVVVAVHGYGRHGRWTDV
                     LLGGGNRELAASMARDLSEHVPGYRFVADLAEIPAALRGLHPDNPVNRPPGGGVQLEL
                     PPRIRGLGPVWADWEGPGLPPPMEALIQGLAAVARSMAARVSPGARPAG"
     gene            1203..1427
                     /locus_tag="VF954_00510"
     CDS             1203..1427
                     /locus_tag="VF954_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529585.1"
                     /translation="MTLAEVIDDHVLRKYRELLDAEDAAFDDLEHAYEEGDRAHFEAD
                     LGAWRAAIVRKLAYLRRLGVEVPEPEAAPV"
     gene            complement(1439..>1871)
                     /locus_tag="VF954_00515"
     CDS             complement(1439..>1871)
                     /locus_tag="VF954_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX9529586.1"
                     /translation="ATQFPDFAHACIEGRPVPEVIDDADWLRSDFITTVQKRGAAVIE
                     ARGLSSAASAANAAVDSVNSIGTPTRAGDWVSLAVVSQGQYGVPEGLVFSYPVRSTGP
                     GHEVVEGIEHDDFAAGKIRLTTEELASERAEVRALGLIPQS"
BASE COUNT          233 a          709 c          679 g          250 t
ORIGIN      
        1 tggccgcctc caccccggcc gcgccggcca ccctggagcg agaccgccgg gtccgccggc
       61 tggtgcgccg ggcccgggcg ctggcgctgg ccgcattcac cgccctgctg gcggcggctt
      121 ccggcctcgg cgtggagatc caggtgcgca gtcacgccca catcccgtcg ctgcacgacc
      181 aggccttcgt gaccgcggcc cggtccgact gcgccctggc cctcgatccg atccgcatga
      241 gccgcccccc agccggcacg gcggagaacg cggcgcggat cgacggcctg gccaccgccc
      301 tggacggagt ggccgcccag atccggaccc ttcccgtcga ggccagggac caggccgcgg
      361 tcacggcatg gttgggagcg tggcagacct actcgagcgt cgcgcaccgg tatgccgacg
      421 ccctgcgctc cggcgccaag gggccgggca gggtcgccca gatggccagc gcggcccggt
      481 cggagatcga cggcttcgcc gaggtcaacc acctgcacgc ctgcgccgtc ggatgaggcg
      541 gtgagcttcg ccgagctcct cgcacacccg gacgtggtcg aggtggtcga gctgcgatcg
      601 agcgtcggct tcctcgcctt tcacgggggc tcgctcgaga aggccaccga cgtggtggcc
      661 gccggggccg cccgccgctc gggcgcctcg ctctacgccg tgcttcagcc acccgggctc
      721 cggtggcaca tcccgtcgca caccgtcgac cccgcccact cagcggcgtt ggcgtcgttc
      781 ctcgaccagg tcgaggtggt cgtggccgtc cacggctacg gccggcacgg gcgctggacc
      841 gacgtgctgc tcggcggagg caaccgggag ctggccgcgt cgatggcccg ggacctcagc
      901 gagcacgtcc ccggctaccg cttcgtcgcc gacctggcgg agatcccggc cgccctccga
      961 ggcctgcatc ccgacaaccc ggtgaaccgc ccgcccggcg gcggcgtcca gctcgagctg
     1021 cccccccgga tccgcggcct gggcccggtc tgggccgatt gggagggtcc gggtctgccg
     1081 ccaccgatgg aggcgctgat ccagggcctg gccgcagtgg cccggtcgat ggccgcccgg
     1141 gtctcgccgg gggcgcgccc tgcggggtaa cgtcagaggt agtcgggaga gaggggatcc
     1201 acgtgacgct ggccgaggtg atcgacgacc atgtgctccg gaagtaccgg gagctgttgg
     1261 acgccgagga cgccgcattc gatgacctcg agcacgccta cgaggagggc gaccgggccc
     1321 acttcgaggc cgatctcggc gcgtggcgag ccgccatcgt gcgcaagctg gcctatctgc
     1381 gtcgcctggg agtcgaggtc cccgaacccg aggccgcccc ggtctagatc gccccggtct
     1441 aggactgggg gatcagcccg agggcgcgca cctcggcccg ctccgaggcc agctcctcgg
     1501 tggtcagccg gatcttcccg gcggcgaagt cgtcgtgctc gatgccctcg accacctcgt
     1561 ggcccgggcc ggtcgaccgc accgggtagc tgaagaccag cccctcgggc accccgtact
     1621 ggccctggct caccaccgcc agcgacaccc agtccccggc ccgggtgggg gtgccgatgc
     1681 tgttgaccga gtcgacggcg gcgttggcgg ccgaggccgc cgaggacagg ccccgggcct
     1741 cgatgacggc ggcgccccgc ttctgcaccg tggtgatgaa gtcgctccgc agccagtcgg
     1801 cgtcgtcgat gacctccggc accggccgcc cctcgatgca ggcgtgggcg aagtccggga
     1861 actgggtggc c
//
LOCUS       DASWGG010000031         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1321111, whole genome shotgun sequence.
ACCESSION   DASWGG010000031 DASWGG010000000
VERSION     DASWGG010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321111"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VF954_00520"
     CDS             <1..576
                     /locus_tag="VF954_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9529587.1"
                     /translation="AAMVDGAAVLATMTYGMRAMGAWKDERGTNLLDTGAPFYDVYET
                     ADGGYISIGSIEPKFYAELLRLSGLDGEELPHQMDASQWPAMKERVAATFRTKTRDEW
                     CALMEHTDVCFAPVLSMAEAATHPHNAERGTFLEVAGVVQPAPAPRFSRTRAEVSRPP
                     AKPGEHTAEALADWGFSADDVAKLRQAGAVA"
     gene            637..1440
                     /locus_tag="VF954_00525"
     CDS             637..1440
                     /locus_tag="VF954_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX9529588.1"
                     /translation="MATLVCFHAHPDDEAIATGGLMVLASDAGHRVVLVTATGGEHGE
                     VADGVLEAGESLGERRAKELAVAARILGVSRTVSLGYVDSGMMGTPENDLPSAFWQAD
                     VEEAAGRLAEVLRDEAADVMTIYDENGVYGHPDHIQVHRVGGRAAELAGTPVVYEATA
                     NRDHVRGLMELAGETPEGPGGPPIDPSDFGVPEARITTRLDVHRAVSRKKEALAAHAS
                     QVPPESFFMTMSEEQFDRAFGDEWFVRVGAPAGTKEDFRLDGISPARPA"
BASE COUNT          228 a          575 c          571 g          195 t
ORIGIN      
        1 gcggccatgg tcgacggcgc cgcggtgctg gcgaccatga cgtacgggat gcgggccatg
       61 ggcgcctgga aggacgaaag gggcaccaac ctgctggaca ccggggcccc tttctacgac
      121 gtctacgaga ccgccgacgg cggctacata tccatcggct cgatcgagcc gaagttctac
      181 gccgagctgc tccggctctc ggggctggac ggggaggagc tgccgcacca gatggacgcc
      241 tcgcagtggc ccgccatgaa ggagcgggtc gcggccacct tccgcaccaa gacgcgcgac
      301 gagtggtgcg cgctcatgga gcacaccgac gtgtgcttcg ccccggtcct gtcgatggcc
      361 gaggccgcga cccatcccca caacgccgag cgcgggacct tcctggaggt ggccggtgtg
      421 gtgcagcccg ccccggcgcc gcgcttcagc cggacccggg ccgaggtgtc gcgccctccc
      481 gccaaaccgg gggagcacac cgcggaggcc ctcgccgatt gggggttctc ggcggacgac
      541 gtcgccaagc tgcgccaagc gggcgccgtc gcttgagctc ccccgcctga gctcccccgg
      601 atgagccggg cgccacgcat gtgccaggat gccgacatgg ccacgcttgt ctgcttccac
      661 gcccatcccg acgacgaagc catcgccacg ggcggtctca tggtcctggc ctcggatgcc
      721 ggccaccgcg tggtgctcgt cacggccacc ggaggagagc acggggaggt tgccgacggt
      781 gtgctggagg ccggcgagtc gctcggcgag cgccgggcca aggagctggc ggtggccgcc
      841 cgcatcctcg gggtgagccg gacggtgagc ctcggctacg tcgactcggg catgatgggc
      901 acccccgaga acgacctgcc cagcgcgttc tggcaagccg acgtcgagga ggccgcgggc
      961 cggctggccg aggtgctgcg ggacgaggcc gccgacgtca tgaccatcta cgacgagaac
     1021 ggcgtgtacg ggcaccccga ccacatccag gtccaccggg tgggcgggcg cgccgccgag
     1081 ctcgccggca ccccggtcgt ctacgaggcc actgccaatc gcgaccacgt gcgcggcctc
     1141 atggagctcg ccggcgagac ccccgaaggg cccgggggcc cgcccatcga tccctcggac
     1201 ttcggggtcc cggaggcgcg catcaccacc cgcctcgacg tccaccgggc cgtgtcccgc
     1261 aagaaggagg cgctcgccgc ccacgccagc caggtcccgc cggagtcgtt cttcatgacg
     1321 atgtcggagg agcagttcga ccgggcgttc ggggacgagt ggttcgtccg ggtcggcgcg
     1381 ccggccggga ccaaggagga cttccgcctg gacggcatct cgccggcgcg cccggcatga
     1441 gccggctgat cctcgtccgg cacggcgagg cggcggcctc atggagcgag gacccggacc
     1501 ccgggctgtc gctggcgggc caccagcagg ccgcagccgt ggccgaagcg ctcgccgccc
     1561 acgccagcc
//
LOCUS       DASWGG010000032         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1645022, whole genome shotgun sequence.
ACCESSION   DASWGG010000032 DASWGG010000000
VERSION     DASWGG010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645022"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VF954_00530"
     CDS             <1..292
                     /locus_tag="VF954_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529589.1"
                     /translation="LDAVARESARAAVEAPPQSAGDVARQVGQDTAAAAGMDTARLEL
                     VPDPTLTRGGTFVVVARYTVSLAPMPGSVTLEVRRAEPVDPFRSFGGVKSSP"
     gene            289..>2031
                     /locus_tag="VF954_00535"
     CDS             289..>2031
                     /locus_tag="VF954_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX9529590.1"
                     /translation="MSEHRPPASRSARLGAVGALVAFCAAPLLMHRLIGFPSFPRLTT
                     WAAMRNFLNHADDAAFGAAVLLWAERILVVLYFYVATELAITLGAQVAERLGRSSLRA
                     ALESMQPRSVAIALNAVVIAALSIAPQVGAGVAAASTPVSAPERPDPAGGGAAPPRAP
                     AAPGSQAQAAQREHVVRSGESLWSIAAAECGSPLYWRDIADLNGVQHPGLIYPGTTMR
                     LPAPCEVPEHQAYVVAAGDTLSRIAQREYGAAGTWEAIWRANRGAVMHDGRTFTDPGV
                     ILPGWTLRLPTHPGPLPSAAAVAPPTPAAGGGAPRQVPTAPPTATPATPPAPPTTPSA
                     AAPVPGPAAAPAAPAASRAHDVAAGHGVRLPFDLLIPPGVASGALALALLARRRRRAN
                     RPLDRPEARRADPPVIGAIRSAGTVPHIDHLAAHTEALSEILTASGHMPRVLGAWDTG
                     ESVRFVLNADPSTLPADGRYDGLGVTVEFTSADGLAVATTTAPGLPSLFRDPVAWTDD
                     LLVPVGCRGSEGWLYLPLLSVPIAVTGDDAAPAVAGMLMAAAIRAGAEELRVLVPEGL
                     ALDAPPEGMDLPALE"
BASE COUNT          224 a          866 c          670 g          271 t
ORIGIN      
        1 cctcgacgcc gtggcccgtg agtccgcccg ggcagccgtc gaggcgccgc cgcaaagcgc
       61 cggcgacgtc gcccggcagg tgggccagga cacggccgcc gcagcgggca tggacaccgc
      121 ccgcctcgag ctggtgcccg acccgactct cacccgcggc ggcacgttcg tcgtcgtcgc
      181 ccgctacacc gtgtcgctcg cgcccatgcc cggctcggtc accctcgagg tccgtcgggc
      241 cgaacctgtc gatccgttca ggagcttcgg gggagtaaag tcgtccccat gagcgagcac
      301 aggcccccgg cctctcgcag cgcccgcctc ggcgctgtcg gtgccctagt cgccttctgt
      361 gccgcaccgc tcctcatgca ccgcctcatc gggttcccgt ccttcccgcg cctcaccacg
      421 tgggccgcga tgcgcaactt cctcaaccac gccgacgatg cggccttcgg cgccgcggtg
      481 ctgctgtggg ccgagcgcat cctggtggtc ctctacttct acgtcgccac cgagctcgcc
      541 atcaccctcg gcgcccaggt cgcagagcgc ctcgggcggt cgtcgctgcg ggccgccctc
      601 gagagcatgc aaccgcgctc ggtcgccatc gccctgaacg ccgtggtgat cgccgccctc
      661 agcatcgccc cccaggtggg tgccggcgtc gccgcggcct cgacccccgt cagcgccccg
      721 gagcgcccgg atccggccgg gggtggtgca gcgccgcctc gagcgcccgc cgcccctgga
      781 tcccaggccc aggccgccca gcgggagcat gtcgtacgct ccggcgagtc cttgtggtcg
      841 atcgccgccg ccgagtgcgg ctcgcccctc tactggcggg acatcgcgga cctcaacggc
      901 gtccagcacc ccggcctcat ctaccccggg acgacgatgc gcctcccggc cccctgtgag
      961 gtccccgagc accaggcata tgtcgtggcg gcgggcgaca ccctctcgag gattgcccag
     1021 cgcgagtacg gcgccgccgg aacgtgggag gccatctgga gagccaaccg cggcgcggtc
     1081 atgcacgacg gccggacctt caccgacccg ggcgtgatcc tgcccgggtg gacgcttcgc
     1141 ctccccacac atcccggtcc cctcccgtct gcggctgcgg tcgccccccc gaccccggcc
     1201 gccggagggg gggccccccg tcaggtgccc acggctccgc ccacggctac gccggccacc
     1261 ccgccggcgc cgcccacgac cccgagcgcc gccgctcccg tgccagggcc ggcggccgcc
     1321 cctgccgcgc cggccgcctc acgcgcccac gacgtcgcag cgggccatgg cgtgcgcctg
     1381 cccttcgatc tccttatccc ccccggcgtg gcctcgggag cgctcgccct cgccctgctc
     1441 gcccgccggc gccggcgggc caaccgcccc ctcgacaggc ccgaggcccg ccgggcggat
     1501 ccgcccgtga tcggcgccat ccgctccgcg gggaccgtgc cgcacatcga ccacctcgct
     1561 gcgcacaccg aggccctgtc ggagatcctc accgcctccg gccacatgcc ccgggtcctc
     1621 ggggcgtggg acacgggcga gtcggtgcgc ttcgtcctga acgccgaccc ctcgaccctt
     1681 cccgccgacg gcaggtacga cggcctcggc gtcaccgtgg agttcaccag cgccgacggc
     1741 ctggcggtgg ccaccaccac ggcgccgggc ctgccctcgc tgttccgcga ccccgtcgcg
     1801 tggaccgacg acctcctcgt gcccgtcggc tgccgggggt ccgagggatg gctgtacctg
     1861 cccctgctgt cggtgccgat tgccgtcacc ggcgacgacg ccgcacccgc cgtggcgggg
     1921 atgctcatgg cggccgctat ccgcgccggg gccgaggagc tgcgggtcct cgtgcccgaa
     1981 ggactcgccc tcgatgcccc gcccgagggc atggacctgc cggcgctcga g
//
LOCUS       DASWGG010000033         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_677351, whole genome shotgun sequence.
ACCESSION   DASWGG010000033 DASWGG010000000
VERSION     DASWGG010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677351"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VF954_00540"
     CDS             <1..497
                     /locus_tag="VF954_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9529591.1"
                     /translation="ALTLAGRDAHIAHVGDSRAYLVRRRVVTQLTADHSRVGEMLRLR
                     LITPEQAANHPARSALTRSLGGDMIVQVDLVTQPVEPGDAFVLCSDGLWDTVPRSELA
                     EILAAIGSPDVPTPAAAAAALVDAAISQKAPDNVTAVVVKVTSDRPIPAAGGRRSLFR
                     RGGR"
     gene            497..1489
                     /locus_tag="VF954_00545"
     CDS             497..1489
                     /locus_tag="VF954_00545"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9529592.1"
                     /translation="MRFAPGQRLDRYEIIDSLGEGAYAETYKARDTETGDVVVLKCPN
                     PQLFADPGLFQRYRREVEVARRLEHPGVQRSMDLGTNRSEPYLVLEYVEGDNLRRRLR
                     AEPGKAPLDLAVALDWGRQLAVALAYLHAHGIVHRDLKPENILVTADDKLKIADFGTA
                     MLEGARRLTWRHLSESLGTPDYMSPEQVQGNRGDARSDLYSWGVLMYELLTGHPPFRG
                     DNMMAVMAGHLQGTPKPITRARPDVPPAVEAVVLHAMRRYPEHRYQSADELVADLDRL
                     DTLDPHAYDNRPEPPMGGMAAAATAARLWLLVAFIALGFLAVVGLIIGLSVALR"
     gene            1495..2715
                     /locus_tag="VF954_00550"
     CDS             1495..2715
                     /locus_tag="VF954_00550"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX9529593.1"
                     /translation="MQPDVDWVRLSFVDVFGATHSVQLPAANFPQAVDHGAPFDGSAL
                     EGRARHLEEDMLLRPDPDTLVDMGERVARVACTVWTPGGRRWPGDPRTALGLVCDDLA
                     ELADGWRASAEMEFYLLDAAGQPVDRGGYFSEVEGAGMAVARSAGSRLAGVGVQVEGC
                     HHESGPGQFEIDVAPLAPLALADALVLVRLVVRDQAAARGLRATFMPRPIDGEAGSGL
                     HLHQRVDGTLAGDGHLSEPGGRFLAGQLAHASGLCALGAPTVNSYKRLHSGPEAPSAA
                     VWAHVNRGALLRVAAGGLEFRGADPLANPYLLIAGLLVAGADGMETDLELPLPEEEDF
                     YGWDPAAYDKARFVPLPRDLDAALDALMADDVLVDAFDGALLSRLVDGRRAEIAEYRA
                     RVTPWELERYFDEG"
     gene            complement(2697..3233)
                     /locus_tag="VF954_00555"
     CDS             complement(2697..3233)
                     /locus_tag="VF954_00555"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX9529594.1"
                     /translation="MRTVVLGPRPAELEALIARRKRLGLDTHDEVWEGEYHVAPEAHL
                     HHAYLDDEVAALLRPLAKRAGLIGTGPFNLGAPDDFRVPDRGLHRSLPPTAWAPTAAL
                     VVEILSPDDETFEKFGFYAAHEVDELLVVDGLRRTLELFARRQDRYERTTASALLAVT
                     SEQLAASIDWPPAQPSSK"
BASE COUNT          455 a         1325 c         1244 g          467 t
ORIGIN      
        1 cggcgctcac cctcgccggg cgcgacgccc acatcgcgca tgtgggcgac tcccgcgcct
       61 acctggtgcg ccgccgcgtc gtgacccagc tcacggccga ccactcccgc gtgggcgaga
      121 tgctgcgcct gcggctcatc acgccggagc aggccgccaa ccatcccgcc cgctcggcgc
      181 tgacccgcag cctcggcggg gacatgatcg tgcaggtcga cctggtgacc cagccggtcg
      241 agccgggcga cgccttcgtg ctgtgctccg acgggctgtg ggacacggtg ccgcgctccg
      301 agctggccga gatcctggcc gccatcggct cgccggacgt gcccacgccg gccgccgcgg
      361 ccgccgccct ggtggacgcg gccatctccc agaaggcccc ggacaacgtc accgccgtcg
      421 tggtcaaggt cacctccgac cggcccatcc ccgcggccgg cgggcgccgg tcgctgttcc
      481 gccggggagg gcgctgatgc gcttcgcccc gggccagcgc ctggaccgct acgagatcat
      541 cgactcgctc ggcgaggggg cctacgccga gacgtacaag gcccgggaca ccgagaccgg
      601 cgacgtagtc gtgctgaagt gccccaaccc ccagctgttc gccgaccccg ggctgttcca
      661 gcgctaccgg cgggaggtgg aggtggcccg ccgcctcgag caccccggcg tccagcgcag
      721 catggacctc ggcaccaacc gcagcgagcc ctacctggtg ctcgagtacg tcgagggcga
      781 caacctgcgc cgccgcctgc gggccgagcc cggcaaggcg cccctcgacc tcgccgtagc
      841 cctggactgg ggccgccagc tcgccgtagc cctggcgtac ctgcacgccc acggcatcgt
      901 gcaccgcgac ctcaagcccg agaacatcct cgtgaccgcc gacgacaagc tcaagatcgc
      961 cgacttcggc acggccatgc tcgagggcgc ccgccgcctg acgtggcgcc acctgtccga
     1021 gagcctcggc accccggact acatgagccc ggagcaggtc cagggcaacc gcggcgacgc
     1081 ccgcagcgac ctctactcgt ggggggtgct gatgtacgag ctgctgaccg gccacccgcc
     1141 gttcaggggc gacaacatga tggcggtcat ggccgggcac ctccagggca cgcccaagcc
     1201 gatcaccagg gcccgccccg acgtaccgcc cgcggtcgag gcggtggtgc tgcacgccat
     1261 gcgccgctac cccgagcacc gctaccagag cgccgacgag ctggtcgccg acctcgaccg
     1321 gctcgacacc ctcgacccgc acgcctacga caaccgcccg gagcccccga tgggcgggat
     1381 ggccgccgcg gccacggccg cccgcctgtg gctgctggtc gccttcatcg ccctcggctt
     1441 cctcgccgtg gtgggcctga tcatcggctt gtccgtggcc ctacgatgat gcccgtgcaa
     1501 ccggacgtcg actgggtccg cctgagcttc gtggacgtct tcggcgccac ccactcggtg
     1561 cagctaccgg ccgccaattt cccccaagcc gtcgaccacg gcgccccctt cgacggctcg
     1621 gccctggagg gacgggcccg gcacctcgag gaggacatgc tgctgcgccc cgaccccgac
     1681 accctggtgg acatggggga aagggtggcc cgggtggcct gcaccgtctg gaccccggga
     1741 ggacggaggt ggccggggga cccccgcacc gcgctcggcc tggtctgcga cgatctggcc
     1801 gagctggccg acgggtggcg ggcgtcggcc gagatggagt tctacctgct ggacgcggcg
     1861 ggccagcccg tcgaccgggg cggctacttc tccgaggtcg agggcgccgg gatggccgtg
     1921 gcccggtccg ccggctcccg cctggccggc gtcggggtgc aggtggaggg ctgccaccac
     1981 gagtccggcc ccggccagtt cgagatcgac gtggccccgc tggccccgct cgccctggcc
     2041 gacgcgctgg tgctggtgcg cctggtggtg cgcgatcagg cggccgcccg ggggctgcgg
     2101 gccacgttca tgccccgccc catcgacggt gaggccggct cggggctgca cctgcaccag
     2161 cgcgtcgacg gcacgctcgc cggcgacggc cacctcagcg agccgggtgg ccgcttcctc
     2221 gctggccagc tcgcccacgc ctctggcctg tgcgccctgg gtgccccgac ggtcaactcc
     2281 tacaagcgcc tgcactccgg gccggaggcg cccagcgcgg cggtatgggc gcatgtcaac
     2341 cggggggcgc tgctgcgggt cgccgccggc ggcctcgagt tccggggcgc cgacccgctc
     2401 gccaacccct acctgctgat cgccgggctg ctggtggcgg gcgccgacgg gatggagacc
     2461 gacctggagc tgccactgcc cgaggaggag gacttctacg gctgggaccc cgccgcctac
     2521 gacaaggccc gcttcgtccc cctgccgcgc gacctggatg ccgccctcga cgccctcatg
     2581 gccgacgacg tgctggtcga cgccttcgac ggcgccctgc tgtcgcgcct ggtcgacggg
     2641 cgccgggccg agatcgccga gtaccgagcc cgcgtcaccc cgtgggagct ggagcgctac
     2701 ttcgacgagg gctgagccgg gggccaatcg atgctggcag ccagctgctc gctcgtcacg
     2761 gccagcaggg cgctggcggt ggtgcgctcg tagcggtcct gccgccgggc gaagagctcg
     2821 agggtgcgcc ggagaccgtc gaccaccagc agctcgtcca cctcatgggc ggcgtagaag
     2881 ccgaacttct cgaaggtctc gtcgtcgggc gacaggatct ccacgaccag tgccgcggtc
     2941 ggcgcccacg ccgtcggcgg gagcgaccgg tgcaggcccc ggtccggcac tcggaagtcg
     3001 tccggcgccc cgaggttgaa cgggcccgtc ccgatcaacc cggcccgctt ggccagcggg
     3061 cggagcagtg ctgccacctc gtcgtcgagg taggcgtggt ggagatgggc ctccggtgcc
     3121 acgtggtact cgccctccca gacctcgtcg tgggtgtcca ggcccagcct ctttcggcgg
     3181 gcgatgaggg cttccagctc ggccggtcgg ggcccgagga ccacggttcg cacgggcgca
     3241 aggatattgg gtccgccgtc gatgtcacac ccctcgggca caatgtcgcc caggtttcag
     3301 ttggcgggat cacacatgga ggtcgacatg ggaaagaggt cgcggcggcg cgcacgcctt
     3361 gaggagggcg cggcatcgcc tcgttgtctg tcggcaggcg agatcgaggt cctgatcggg
     3421 aacgcgctgg cggtcggagg gcacgacgcg ctggcgcgcc tggccgccga ctggcgctgg
     3481 ccccagcgcg a
//
LOCUS       DASWGG010000034         5973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1322341, whole genome shotgun sequence.
ACCESSION   DASWGG010000034 DASWGG010000000
VERSION     DASWGG010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322341"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1443
                     /locus_tag="VF954_00560"
     CDS             109..1443
                     /locus_tag="VF954_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529595.1"
                     /translation="MAGGRELAAVIASDAVRLGGRSLSAALGHARAGALAAPSVRGKG
                     DPEADSVVPTDPTTLALALAAGRPPLERAVVDLWARHGMDGGAVARALGTAPGDAKAR
                     LGAVSAAWQEDLDPALMAWLGPGECDELSAVLEREGIGPAGAEPTVGALLSGAPAVAA
                     HAGACETCRDRQRAMVSVQSLVGQVPLPPAPPQVRTMAAAARRRRPVPPPPLSPPRRS
                     RWPRRVALGTAVLAATAGAVVAATASIVAWSPDAVAQRVRNITRVRTDALSLTPDVLG
                     PASSSAVLTNRSARPEAWRATASVPWLTVTPPGGTIGPGERLTLRIVFAAAAPQGALH
                     GTLTVTSPDGSVAAAALTGILDRPPQLDAELSGCTVTATAEDSDGLAAVLLDWQDLAG
                     THQVAMASVGAAYTAQLPPAGTWWVTAVDTLGNVATLPPRPAPALPCPAAAS"
     gene            complement(1498..4077)
                     /gene="rpoC"
                     /locus_tag="VF954_00565"
     CDS             complement(1498..4077)
                     /gene="rpoC"
                     /locus_tag="VF954_00565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX9529596.1"
                     /translation="MGPTLKLHQCGLPKQMALELFKPFVMKRLVDTELAQNIKSAKRM
                     VERRRTQVWDVLEEVIKEHPVLLNRAPTLHRLGIQAFEPILVEGKAIQIHPLVCAAFN
                     ADFDGDQMAVHLPLSAEAQAEARVLMLSANNILSPATGRPITVPSHDMVFGAYYLTVD
                     RTGAPGTGRTFRHLYEVLHAYECGDLGLQALIKVRTAVAAGDGEVAADELETTAGRLI
                     FNTALPADFPFVNELVSKRTRSIGSIVEELASNYSKAVVAESLDQIKDLGFRFATQSG
                     LTISIDDVKTPPEKGAIIDRFEKEAEKAETQFKRGIITDDERRQKEIEIWTSANSEVG
                     KAMDLTLSHMAFNPLDMMVNSGARGNPQQVRQIAGMKGLVSNPRGEMIPRPIISSFRE
                     GLSVLEYFISTHGARKGLADTALRTADSGYLTRRLVDVAQELIVREDDCGTARGIWLE
                     DVTPDQAGKRSYLETRIYGRVLVEPVTIGDGTTFDAGIVVGDDEMAALRDDPTVTRAR
                     VRSVLTCEAKYGICAMCYGRSLATGREIELGEAVGVIAAQSIGEPGTQLTMRTFHTGG
                     IAGEDITHGLPRVVELFEARTPKGAAILARTSGVVRVAEEENGRRITIVGDDGSEDVY
                     NVVTRAHLEVTDGQEIAAGDALVEGPKDPKELLDIKGIRETQQYLVEEVQKVYRDQGV
                     SIHDKHIELIVRQMLRRVLVADAGEATFLPGERVDSRTYAEVNRALVQEGKRPAEGRP
                     ELMGITKASLATESWLSAASFQETTRVLTEAAIEGKSDQLFGLKENIIIGKLIPAGTG
                     MTRYRSVETEAPDAERMTFWSSETEPETEDLAAWLATIGTDGADDKKQAAETWGGGAP
                     ESA"
     gene            complement(3999..5387)
                     /locus_tag="VF954_00570"
     CDS             complement(3999..5387)
                     /locus_tag="VF954_00570"
                     /inference="COORDINATES: protein motif:HMM:NF016858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529597.1"
                     /translation="MLDVNNFDQLRIGLATAEQIRGWSNGEVKKPETINYRTLKPEKD
                     GLFCEKIFGPTRDWECYCGKYKRVRFKGIICERCGVEVTRSKVRRERMGHIELAAPVT
                     HIWYFKGVPSRLGYLLDLAPKNLEKIIYFAAHLITWVDDERRHRDLSSLEAKHRSEIE
                     QVERDRDLWIDERFKALEGDLEELEGSGAKDSELNKLRKAAEKEIGDYRDRRNNEIEH
                     LKTVWDTFNSLAPKQLIDDERVWRDLQERYAEYFAGGMGAQAVEELISRLDLSAEEAF
                     LRETIATGKGQKKAKAIKRLKVVSAFNRRNDEDRLVNSPTGMVLRAVPVIPPDLRPMV
                     QLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPEIIVNNEKRMLQEAVDALFDNGR
                     RGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGSRSSWWVPRSSCTSAGCP
                     SRWRWSCSSRSS"
     gene            complement(5423..>5973)
                     /locus_tag="VF954_00575"
     CDS             complement(5423..>5973)
                     /locus_tag="VF954_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX9529598.1"
                     /translation="APNGKAQLFDGRSGRPYDRPTTVGYVYILKLAHLVDDKIHARST
                     GPYSMITQQPLGGKAQFGGQRLGEMEVWALEAYGAAYALQELLTIKSDDVLGRVKVYE
                     AIVKGENIPEPGIPESFKVLIKEMQSLCLNVEVISTTGEEIEMRELDEDVFRTAEELG
                     IDLSRPEKGSDEEDERRREPSF"
BASE COUNT          745 a         2083 c         2164 g          981 t
ORIGIN      
        1 tggccggcgg tccatgttac gggagcggcg ggcggcgggc aaccggagta ttgtcaccgc
       61 gtcatggcca cggatcgggc tgcgtcggcc tatggccacg ctttggccgt ggccggcggc
      121 agggagctgg ccgccgtcat cgcgtccgac gccgtgcgcc tcggcggccg ctcgctctcc
      181 gcggccctgg gccacgcccg cgccggggct ctggccgcgc ccagcgttcg gggaaagggt
      241 gaccccgagg ccgactcggt cgtgcccacc gatccgacca ccctggccct ggcgctggcg
      301 gccgggcgcc cacccctcga gcgggccgtg gtcgacctct gggcccggca cggcatggac
      361 ggcggcgcgg tggcccgggc cctcggcacc gctccgggcg acgccaaggc gcgcctcggc
      421 gcggtgtcgg cggcgtggca ggaggacctg gaccccgccc tcatggcgtg gctcgggccc
      481 ggcgagtgcg acgagctctc ggcggtgctg gagcgagagg gcatcggccc ggcgggagcc
      541 gagccgaccg tcggcgcgct tctgtcgggc gcgcccgcgg tggccgccca cgccggggcg
      601 tgcgagacct gccgtgaccg gcagcgggcg atggtgtcgg tccagagcct ggtgggccag
      661 gtgcccctgc caccggcgcc cccccaggtg aggacgatgg ccgccgccgc ccgccggcgc
      721 cggcccgtac cgccccctcc gctctcgccc ccccggcgct cccggtggcc gcgccgcgtc
      781 gctctgggga ccgcggtgct ggccgccacc gccggcgccg tggtggcggc gaccgcgtcg
      841 atcgtggcgt ggagcccgga cgccgtcgcc cagcgggtgc gcaacatcac ccgggtgcgc
      901 accgatgcct tgtccctcac ccccgacgtg ctcggcccgg ccagctcctc ggccgtgcta
      961 accaaccggt ccgcccggcc ggaggcgtgg cgggcgacgg cgtcggtgcc ctggctcacc
     1021 gtcacgccgc cggggggcac catcggcccg ggcgagcgcc tgacactgcg gatcgtgttc
     1081 gccgcagccg cgccccaggg agctctccac ggcacgctga cggtgaccag cccggacggg
     1141 tcggtggcgg cggccgccct gaccggcatc ctcgaccgcc cgccgcagct ggacgccgag
     1201 ctgtcgggct gcaccgtgac ggccaccgcc gaggacagcg acgggctggc cgcggtgctg
     1261 ctcgactggc aggacctggc cggcacccac caggtcgcca tggcctcggt gggcgcggcc
     1321 tacacggcgc agctcccccc ggccgggacg tggtgggtga cggcggtgga caccctgggc
     1381 aacgtcgcga ccctgccccc gcggccggcg ccggcgctcc cctgcccggc tgcggcgtcc
     1441 tgacggctca ggagatcggg cggcgcgacg gccgcccttt ctcccgagcc cgccagctca
     1501 tgccgactcg ggggcgccgc cgccccaggt ctcggcggcc tgcttcttgt cgtcggcgcc
     1561 gtcggtgccg atcgtcgcca gccaggcggc caggtcctcg gtctcgggct cggtctccga
     1621 ggaccagaac gtcatgcgct cggcgtcggg cgcctcggtc tcgaccgagc ggtaccgggt
     1681 catgcccgtc ccggccggga tgagcttgcc gatgatgatg ttctccttga gaccgaagag
     1741 ctggtcggac ttgccctcga tggcggcctc ggtgaggact cgggtggtct cctggaagga
     1801 ggccgccgac agccacgact cggtggccag cgacgccttg gtgatgccca tcagctcggg
     1861 gcggccctcg gccgggcgct tgccctcctg gacgagggcc cggttgacct cggcgtaggt
     1921 ccgggagtcc acgcgctcgc ccggcaggaa ggtggcctcg ccggcgtcgg ccaccagcac
     1981 gcgccgcagc atctggcgga cgatcagctc gatgtgcttg tcgtggatcg acacgccctg
     2041 gtcccggtac accttctgga cctcctcgac gaggtactgc tgggtctcgc ggatgccctt
     2101 gatgtccagc agctccttgg ggtccttggg cccttcgacc agggcgtcgc cggccgcgat
     2161 ctcctggccg tcggtgacct ccaggtgcgc ccgggtgacc acgttgtaga cgtcctcgga
     2221 cccgtcgtcg cccacgatgg tgatgcggcg gccgttctcc tcctcggcga cgcgcaccac
     2281 gcccgaggtc cgggccagga tcgccgcgcc cttgggcgtg cgggcctcga acagctcgac
     2341 gacgcgcggc aggccgtggg tgatgtcctc accggcgatg cccccggtgt ggaaggtccg
     2401 catggtcagc tgggtgccgg gctcgccgat ggactgggcc gcgatgaccc cgacggcctc
     2461 gcccagctcg atctcccggc cggtggccag cgaccggccg tagcacatgg cgcagatgcc
     2521 gtatttggcc tcgcaggtca gcaccgagcg gacccgggcc cgggtgacgg tcggatcgtc
     2581 gcggagggct gccatctcgt cgtcgccgac gacgatgccg gcgtcgaagg tggtcccgtc
     2641 cccgatggtg accggctcga ccaggacccg gccgtagatg cgggtctcga ggtacgagcg
     2701 cttgcccgcc tggtcggggg tcacgtcctc gagccagatg cctcgggccg tgccgcagtc
     2761 gtcctcccgc acgatcagct cctgggccac gtcgaccagc cgccgggtca ggtagccgga
     2821 gtcggccgtc cgcagggcgg tgtcggccag gcccttgcgc gcgccgtggg tggagatgaa
     2881 gtactcgagg accgacagcc cctcccggaa ggaggagatg atgggccggg ggatcatctc
     2941 gccccggggg ttggacacca ggcccttcat gccggcgatc tggcggacct gctgcgggtt
     3001 gccgcgggcg ccggagttga ccatcatgtc gagcgggttg aaggccatgt gcgacagggt
     3061 gaggtccatg gccttgccca cctcggagtt ggccgaggtc cagatctcga tctccttctg
     3121 gcgacgctcg tcgtcggtga tgatgccccg cttgaactgc gtctcggcct tctcagcctc
     3181 cttctcgaag cggtcgatga tggcgccctt ctcgggcggc gtcttgacgt cgtcgatgga
     3241 gatggtcagg cccgactggg tcgcgaaccg gaagcccagg tccttgatct ggtcgaggct
     3301 ctcggccacc acggccttcg agtagttgga ggccagctcc tcgacgatgg acccgatgga
     3361 ccgggtccgc ttgctgacca gctcgttgac gaacgggaag tcggcgggca gggcggtgtt
     3421 gaagatgagc cgcccggcgg tggtctccag ctcgtccgcg gcgacctcgc cgtcgccggc
     3481 ggccacagcc gtccgcacct tgatcagggc ctggagcccc aggtccccgc actcgtaggc
     3541 gtgcagcacc tcgtagaggt gccggaaggt ccggcccgtc cccggcgccc cggtccggtc
     3601 cacggtcagg tagtaggccc cgaacaccat gtcgtgggac ggcacggtga tggggcggcc
     3661 ggtggcgggc gacaggatgt tgttggccga cagcatgagg acgcgggcct cggcctgggc
     3721 ctcggccgac aggggcaggt gcacggccat ctggtcgccg tcgaagtcgg cgttgaaggc
     3781 ggcgcacacg agcgggtgga tctggatggc cttgccctcg accaggatgg gctcgaaggc
     3841 ctggatgccg aggcggtgca gggtcggggc ccggttgagc agcaccgggt gctccttgat
     3901 gacctcctcg agcacgtccc acacctgggt ccggcggcgc tcgaccatgc gcttggccga
     3961 cttgatgttc tgggccagct cggtgtcgac gagccgcttc atgacgaacg gcttgaacag
     4021 ctccagcgcc atctgcttgg gcagcccgca ctggtgcagc ttgagcgtgg gacccaccac
     4081 gatgaccgac tgcccgagta gtcgacccgc ttgcccagca ggttctggcg gaagcggccc
     4141 tgcttgccct tgagcatgtc ggacagcgac ttgagcgggc ggttgcccgg ccccgtgacc
     4201 gggcggcccc gccggccgtt gtcgaagagg gcgtcgacgg cctcctggag catgcgcttc
     4261 tcgttgttca cgatgatctc gggcgcgccc aggtcgagga gccgcttcag gcggttgttg
     4321 cggttgatca cccggcggta gaggtcgttc aggtcggagg tggcgaagcg gccaccgtcg
     4381 agctggacca tgggccgcag gtcgggcggg atgaccggga cggcgcgcag caccatgccg
     4441 gtgggcgagt tgaccagccg gtcctcgtcg ttgcggcggt tgaaggccga cacgaccttg
     4501 aggcgcttga tggccttggc cttcttctga cccttgccgg tggcgatcgt ctcccggagg
     4561 aaggcttcct cggcggacag gtcgaggcgg gagatgagct cctccacggc ctgggcgccc
     4621 atgccgccgg cgaagtactc ggcgtaacgc tcctggaggt cgcgccacac ccgctcgtcg
     4681 tcgatgagct gcttgggggc cagcgagttg aacgtgtccc acaccgtctt caggtgctcg
     4741 atctcgttgt tgcgccggtc ccggtagtcg ccgatctcct tctccgccgc cttgcggagc
     4801 ttgttgagct cggagtcctt ggccccggag ccctcgagct cctcgaggtc accctcgagg
     4861 gccttgaagc gctcgtcgat ccacaggtcg cggtcccgct ccacctgctc gatctccgag
     4921 cggtgcttgg cctccagcga cgaaaggtcg cggtggcggc gctcgtcgtc gacccaggtg
     4981 atcaggtggg cggcgaagta gatgatcttc tcgaggttct tcggggccag gtcgaggagg
     5041 tagccgagcc gcgacgggac gcccttgaag taccagatgt gggtgaccgg ggcggccagc
     5101 tcgatgtggc ccatccgctc ccggcggacc ttcgagcggg tgacctcgac gccgcaccgc
     5161 tcacagatga tgcccttgaa gcggacgcgc ttgtacttcc cgcaatagca ctcccagtct
     5221 cgggtgggac cgaagatctt ctcgcagaag agcccgtcct tctccggctt gagcgtgcgg
     5281 tagttgatcg tctccggctt cttgacctcg ccgttggacc atccccggat ctgctcggcc
     5341 gtggccaggc cgatgcggag ctgatcgaag ttgttgacgt cgagcactgg gtgcttcgcc
     5401 tcctaaatac ggttcgtgcc gactagaagc tgggctcgcg tcgccgctcg tcctcctcgt
     5461 cactgccctt ttcgggccgg gacaggtcga tgccgagctc ctcggccgtc cggaagacgt
     5521 cctcgtcgag ctcccgcatc tcgatctcct cgccggtcgt cgagatgacc tcgacgttga
     5581 ggcacaggga ctgcatctcc ttgatcaaca ccttgaagga ctcggggatc cccggttcag
     5641 ggatgttctc ccccttcacg atggcctcgt agaccttgac ccgtccgagc acgtcgtcgg
     5701 acttgatggt cagcagctcc tggagggcgt aggcggcgcc gtaggcctcc agggcccaga
     5761 cctccatctc gccgaggcgc tgcccgccga actgcgcctt cccgccgagg ggctgctggg
     5821 tgatcatcga gtaggggccg gtcgaccggg cgtggatctt gtcgtcgacc aggtgggcca
     5881 gcttcaggat gtagacgtag ccgacggtgg tcgggcggtc gtagggccgg ccggagcgcc
     5941 cgtcgaagag ctgggctttg ccgttggggg cga
//
LOCUS       DASWGG010000035         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2612299, whole genome shotgun sequence.
ACCESSION   DASWGG010000035 DASWGG010000000
VERSION     DASWGG010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2612299"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VF954_00580"
     CDS             complement(<1..1014)
                     /locus_tag="VF954_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX9529599.1"
                     /translation="MTTIAGLRERLLAGDVTPSDAVDAAVAALADGDAATVISLVAPD
                     HLRARLRALDGIDPTSHPLWGIPFAVKDNIDVAGAPTTAACGPFSYHPATSARVVRQL
                     EAAGAIPVAKTNLDQFATGLVGTRSPYGTPANPWDPARVPGGSSSGSAVAVARGAVPF
                     ALGTDTAGSGRVPAAYNGVVGAKPTRGWLSTGGVVPAVRTLDCVSVLALSVGDAWTVV
                     RAAGGYDADDPFSRVPAASPGRSRRLVGVAGGVPLAADLSGCGPVRALGDDVLPRLLA
                     AGRLLYDGPWVAERLAALGPFVDAHPSDVDPAVAAIFSAAGRWTATDAALARYELAGL
                     ARQL"
     gene            complement(1011..>2417)
                     /locus_tag="VF954_00585"
     CDS             complement(1011..>2417)
                     /locus_tag="VF954_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HEX9529600.1"
                     /translation="EAERWKLGQLTAGDRVSFVRLDPAAPASPATWPAGWQRRARPVD
                     PVVGLRPGQGGDAPAVTYRRAGDTHLLVEYGDNVLDLALRFRVHALMQALEVARVVGV
                     LELTPGIRSLQLRYDPAVLPLAALLEMLADVEDGLADPCSAAVPSRIVHLPLSWDDPA
                     TRVAIQRYMQVVRDDAPWCPWNLEFIRRVNGLDDIAAVREIVYGASYLVLGLGDVYLG
                     APVATPLDPRHRLVTTKYNPARTWTPENAVGIGGAYLCIYGMEGPGGYQLVGRTIQVW
                     NRYRDTPDFAGSQRWLLRFFDQLRFFPVSHEELLDLRRDFPVGRYQVATEDTTLSLAA
                     YRSFLADHAPSIATFKRRQQAAFDAERSRWEESGELTRGAQLAGAALDAALDGVDDTM
                     PLPAGAHGIFTPLHGVVARIAAAGDEVERGDPVAVVEAMKTETEITTPAEGTVTEVRC
                     RVGDVVRPGVPLAVVSPR"
BASE COUNT          360 a          872 c          891 g          294 t
ORIGIN      
        1 caactggcgg gccaggccgg cgagctcgta gcgggccagg gcggcatccg tcgccgtcca
       61 ccgccccgcg gccgaaaaga tggccgccac cgccggatcg acgtccgagg gatgagcgtc
      121 cacgaacggc ccgagagccg ccaggcgctc cgccacccag gggccgtcgt agagcagccg
      181 gccggctgcc agcagccggg gcagcacgtc gtcaccgagg gcccgcaccg gcccgcagcc
      241 ggagagatca gcagccaacg gcaccccccc ggccacgccg acgaggcgtc gggatcggcc
      301 cggcgacgcg gccggcaccc ggctgaaagg atcatcggcg tcgtacccgc ccgccgcccg
      361 caccaccgtc cacgcgtcac caaccgagag agcgagcacg ctgacgcagt cgagcgtccg
      421 gacggcgggc acgacgccgc ccgtgctgag ccagccccgg gtcggcttgg cgccgacgac
      481 gccgttgtag gcggccggca cccgccccga cccggcggtg tccgtgccga gggcgaaggg
      541 caccgcgccc cgtgccacgg ccacggccga tcccgagctg gaccctccgg gcacccgggc
      601 cgggtcccag gggttggccg gcgtgccgta cgggctgcgg gtgcctacca ggccggtcgc
      661 gaactggtcg aggttggtct tggccacggg gatggcgccg gccgcctcca gctgacggac
      721 cactcgagcc gaggtggcgg gatggtagga gaaagggcca catgctgcgg tcgtaggcgc
      781 cccggccacg tcgatgttgt ccttgacggc gaagggaatg ccccaaaggg gatggctggt
      841 cgggtcgatc ccgtccaggg ctcggagccg agcccttaag tggtcgggtg cgaccaggct
      901 gatgacggtc gccgcgtccc catccgccag ggctgcgacg gccgcgtcga cggcgtccga
      961 cggcgtcacg tcgcccgcga gaagccgctc gcgcaaccca gcgatggtcg tcaccgggga
     1021 ctgaccacgg cgagcgggac gccgggtcgc acgacgtcgc ccacccggca gcggacctcc
     1081 gtgacggtac cttccgccgg agtggtgatc tcggtctcgg tcttcatggc ctccacgacc
     1141 gcgacagggt ccccccgctc gacctcgtcg ccggcggccg cgatccgcgc caccacgccg
     1201 tgcagcgggg tgaagatgcc gtgggcgccg gccgggaggg gcatggtgtc gtcgacaccg
     1261 tccagggccg cgtcgagcgc cgcgccggcc aactgggcgc cgcgggtcag ctcaccgctt
     1321 tcttcccacc gggagcgctc ggcgtcgaag gccgcctgct ggcggcgttt gaaggtggcg
     1381 atcgacggcg cgtggtcggc caggaagctc cggtaggcgg ccaggctcaa ggtcgtgtcc
     1441 tcggtcgcca cctggtaccg gccgacgggg aagtcgcgcc ggagatccag cagctcctcg
     1501 tggctgacgg ggaagaagcg aagctggtcg aagaagcgca gcagccaccg ctgcgagccc
     1561 gcgaagtcgg gcgtgtcgcg gtagcggttc cacacctgga tggtccgccc gaccagctgg
     1621 tagccgcctg gtccctccat cccgtagatg cagaggtagg cgccgccgat gccgacggcg
     1681 ttctccggcg tccaggtccg cgccgggttg tacttggtcg tgaccagccg gtggcgggga
     1741 tcgagcggag tcgccaccgg ggcgcctagg tacacgtcac cgaggcccag cacgagatag
     1801 gacgcgccgt acacgatttc acgcacggcc gcgatgtcgt ccaggccgtt gactctgcgg
     1861 atgaactcca ggttccacgg gcaccacggc gcgtcgtcgc gcacgacctg catgtaccgc
     1921 tggatggcta cccgggtggc ggggtcgtcc caggagagcg ggaggtgcac gatacggctc
     1981 gggacggccg ccgagcacgg atcggcgagc ccgtcctcca cgtcggcgag catctcgaga
     2041 agggcggcga gcgggaggac cgcggggtcg taccggagct gcagggatcg gataccggga
     2101 gtcagttcga gcacgcccac cacccttgcc acctccaggg cctgcatcag ggcatgcacg
     2161 cggaaccgca gggccaggtc gagcacgttg tcgccgtact ccacgagcag gtgggtgtct
     2221 cctgcccggc ggtacgtaac cgccggggca tcgccaccct gtccggggcg gagccccacc
     2281 acggggtcga caggacgagc gcgccgctgc caacccgccg gccatgtagc cggcgaagcc
     2341 ggcgccgccg ggtcaaggcg gacgaagctg acccggtccc cggcggtcag ctgccccagc
     2401 ttccagcgct cggcctc
//
LOCUS       DASWGG010000036         2298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_679492, whole genome shotgun sequence.
ACCESSION   DASWGG010000036 DASWGG010000000
VERSION     DASWGG010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679492"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VF954_00590"
     CDS             complement(<1..330)
                     /locus_tag="VF954_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9529601.1"
                     /translation="MSLSAAVELTLGALHLDVEMAVGEAAVVVVVGPNGAGKTTLVRA
                     LAGLVALDRGRVVIDGTVVEDPATGVREPPERRGVGVVFQEPRLLAHLSAVDNVAFGL
                     RATGRPKG"
     gene            complement(327..1091)
                     /locus_tag="VF954_00595"
     CDS             complement(327..1091)
                     /locus_tag="VF954_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9529602.1"
                     /translation="MPGVLAAGAVALVVLPLVSLLQRAPWSALAGDLRAHDAFAALRL
                     SLETSLGALAVSATFGVPLAWVLARRRFPGRSLVRALVTLPMVLPPVVGGIALLYAFG
                     RRGLAGRALYDLTGWRLVFNTSGAIVAEAFVAMPFLIVTAEAAFRSMDRKAEEAAATL
                     GASPAYRFRRVVLPAVAPALAAGAALTWARALGEFGATITFAGSFPGTTQTTPLLVYL
                     DIESGHEGTALALSLVLLGVSLAVLVGLRHRWLGHP"
     gene            complement(1117..1908)
                     /gene="modA"
                     /locus_tag="VF954_00600"
     CDS             complement(1117..1908)
                     /gene="modA"
                     /locus_tag="VF954_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9529603.1"
                     /translation="MRTGARRRGVLGLLTAVALAAPACSSRTTPPSSARAAPITGSLN
                     VLAAASLTAAFNRAKTALTTADPGLTLTYDFAGSNTLVSQITQGAPADVFASADANNM
                     QRLLDAGLVDPPVTFARNKLEIAVAPGNPKHVTGLADLARPGVSVVLEAPGVPAGDYT
                     RLALTAEHLTVTPRSLEADVRSALAKIASGEADATVVYVTDVAAAGATVTGVTVPDAD
                     QAGITYPIAVVKATRNRAAAQAFVDSAVSGDVQRALEAAGFLPPR"
     gene            complement(1905..>2298)
                     /locus_tag="VF954_00605"
     CDS             complement(1905..>2298)
                     /locus_tag="VF954_00605"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9529604.1"
                     /translation="PTFTVGQAAELLGVSPDTVRRWMDSGQLPAARTPGGRRRVEGAT
                     LARFSADRAAAPSFLPVAESARNHFPGLVTKVTKDKVMAQVEIQAGPYRVVSLMSREA
                     AEELALAPGVLAVASVKSTNVVVEVPQP"
BASE COUNT          328 a          824 c          901 g          245 t
ORIGIN      
        1 gcccttgggc cggccggtgg cccgcagccc gaaggcgacg ttgtcgaccg cgctgaggtg
       61 ggcgagcagc cggggctcct ggaacacgac cccgacgccg cgccgctccg gcggctcccg
      121 aacaccggtt gccgggtcct cgaccacggt cccgtcgatg acgacccggc cccggtcgag
      181 ggccaccagc ccggccaggg cccgaacgag ggtggtcttg ccggcgccgt tggggccgac
      241 gacgacgacg actgccgcct cgcccaccgc catctcgacg tcgaggtgca gcgcgcccag
      301 ggtcagctcg accgcggcgg acaggctcac gggtggccga gccaacggtg ccgaagcccc
      361 accagcacgg ccagggacac gccgagcagc accagcgaca gggccagggc cgtcccctcg
      421 tggccggact cgatgtcgag gtagacgagc agcggcgtgg tctgggtggt gccggggaag
      481 ctgccggcga aggtgatggt ggccccgaac tcgcccaggg cccgggccca cgtgagcgcg
      541 gcgccggcgg ccagggccgg agccacggct ggcaggacga cgcgccggaa ccggtacgcc
      601 gggctcgccc ccagggtggc ggcggcctcc tcggccttgc ggtccatgga ccggaaggcg
      661 gcctcggcgg tcacgatcag gaagggcatg gccacgaacg cctcggccac gatggccccg
      721 ctggtgttga acaccagccg ccagccggtc aggtcgtaca gggcacgccc ggcgaggccg
      781 cgccggccga aggcgtacag cagggcgatg ccgccgacca ccggcggcag gaccatcggc
      841 agggtgacca gggcgcgcac cagggaccgg cccgggaagc ggcgccgggc caggacccac
      901 gccaggggca cgccgaaggt ggcggacacg gcgagggcgc cgagcgaggt ctcgagggac
      961 aggcgcaggg cggcgaaggc gtcgtgggcc cggaggtcgc cggccagcgc cgaccacggg
     1021 gctcgctgga gcagggagac gagcggcagc acgaccaggg cgacggcccc ggcggccagg
     1081 accccgggca ccaccgcgaa cggctgccgc cgagggctac cgcggcggga ggaaaccggc
     1141 cgcctccagg gcccgctgca cgtcgccgga gacggctgag tcgacgaagg cctgggccgc
     1201 agcccggttg cgggtggcct tgacgaccgc gatcgggtag gtgatgccag cctggtcggc
     1261 gtcgggcacc gtcacccccg tgacggtggc gccggctgcg gccacgtcgg tcacgtacac
     1321 gaccgtcgcg tcggcctcgc ccgaggcgat cttggccagg gccgagcgga cgtcggcctc
     1381 caacgaccgg ggcgtgaccg tcaggtgctc ggcggtgagc gccagccggg tgtagtcacc
     1441 ggccggcacc ccgggcgcct ccagcaccac gctcacgccg ggccgggcca ggtcggcgag
     1501 gccggtgacg tgcttggggt tgccgggagc caccgcgatc tccagcttgt tcctggcgaa
     1561 ggtgaccggc gggtccacca agccggcgtc gagcagccgc tgcatgttgt tggcgtcggc
     1621 cgaagcgaac acgtccgcgg gcgcgccctg ggtgatctgg gagaccaggg tgttggagcc
     1681 ggcgaagtcg taggtaaggg tcaggcccgg gtcggcggtc gtcaacgccg tcttggcccg
     1741 gttgaacgcc gcggtcagcg atgccgccgc caacacgttc agcgatcctg tgatcggcgc
     1801 cgcccgggcg ctcgacggtg gcgtcgtcct ggacgagcag gccggcgcgg ccagcgccac
     1861 cgcggtcagc aacccgagga cgccgcggcg gcgagcgccg gtcctcacgg ctgggggacc
     1921 tcgaccacga cgttggtgga tttcaccgat gccacggcca acacccccgg ggcaagggcg
     1981 agctcctcgg ccgcctcacg gctcatgagg gacacgaccc ggtaggggcc ggcctggatc
     2041 tcgacctggg ccatgacctt gtccttggtc accttggtca ccagcccggg gaagtggttg
     2101 cgggccgact cggcgaccgg caggaacgac ggggcggccg cccggtcggc cgagaaccgg
     2161 gcgagggtgg cgccctcgac ccggcgccgt ccccccggcg tgcgagccgc cgggagctgg
     2221 ccgctgtcca tccagcgcct gaccgtgtcg gggctgaccc cgagcagctc ggccgcctgg
     2281 ccgacggtga aggtgggc
//
LOCUS       DASWGG010000037         4329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1325514, whole genome shotgun sequence.
ACCESSION   DASWGG010000037 DASWGG010000000
VERSION     DASWGG010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325514"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VF954_00610"
     CDS             <1..547
                     /locus_tag="VF954_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX9529605.1"
                     /translation="LESCPGDLSGIHSVASFFVSRVDTEVDRRLNAVGDPAAGLAGKA
                     AVAQARLAYQLFRQRFSGARWEALAARGAHLQRPLWASTSTKNPAYPDLLYVDNLIGP
                     HTVNTMPDATIDAFLDHGAVARTVDQDADAAQADLARLATLGVDLGDVARTLEEEGVA
                     AFSKSFDELIQALTDKANSLG"
     gene            540..1892
                     /locus_tag="VF954_00615"
     CDS             540..1892
                     /locus_tag="VF954_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529606.1"
                     /translation="MGEALRRLLARDATLWPAGNVAANRLGWLDLPRRMAGEARELAA
                     FAASVDQPKVVLLGMGGSSLGPEVLRASRPGPGRRLVVLDTTDPQTVAGVDLSDAFFL
                     VSSKSGTTLEVQAHFAHAWAQVPDGSRYAAITDPGTPLAALAAAHGFRRTFENPPDIG
                     GRYSVLSYFGLVPAALLGYDVAELCERALGTDLEDAAELGVAMGEAARQGRDKVTVVV
                     PKPFRAFGLWVEQLIAESTGKRGTGCVPVPTTEVEEGPDRHVVALEIGTADDLAAEFY
                     RWELATAIAGHVLGIDPFDEPNVAESKANTRQVLDSLPLPQLDHADPATVSDWLRRTV
                     RPNDYVSVQAYLPYGHDDDLEELRRTLRDGLGGAAVTAGYGPRFLHSTGQLHKGGPDE
                     VVAVQLVRRTHVPSVAIPGFPYDYDTLIAAQAIGDHRSLLDHGRRVLRVAVDSPHEVG
                     "
     gene            1895..3421
                     /gene="zwf"
                     /locus_tag="VF954_00620"
     CDS             1895..3421
                     /gene="zwf"
                     /locus_tag="VF954_00620"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX9529607.1"
                     /translation="MAAVTTDLPNPLLMGLEGERRAPPCVLVVFGASGDLASRKLLPA
                     IEQLSRHRRLPANFAVVGVARTDMTDEQFRRMCLDAVPGAGPAWDGLVAGFRYVAGEY
                     ARGETFDTLSRVLAEVDRDRGTAGNRVHYLATIPAVFGAVADALAHHGLNQAGEDGGF
                     VRVVVEKPFGRDLESARALDAELHTAFDESQIYRIDHYLGKETVQNVLALRFANAIFE
                     PIWNRRYVEQVQITVAECLGVEHRGGFYETAGALRDIVQNHVLQVLALTLLEPPATVD
                     AQGIRDEKVKALRAVKILDPSEVPAYVVRGQYDTGWIEGAEVPGYRAEADVAPDSETE
                     TYLAMRLDVDNWRWAGVPIYARTGKRLAKRVTEVAMQFHSVPHLPFASKLSEGLMPNA
                     LVVRVQPDEGITLRFGAKVPGQEFRVRSVSMDFSYGAAFLEETPEAYERLLLDAMAGD
                     PTLFIRTDEVEQAWRIADPILEAFGGRQPPLARYPAGSWGPREADALIARDGHAWRTP
                     "
     gene            3406..>4329
                     /locus_tag="VF954_00625"
     CDS             3406..>4329
                     /locus_tag="VF954_00625"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX9529608.1"
                     /translation="MADPVAAPLELGSWSGTGVRVGDVERALEDLRHGEQRAATRTSV
                     VTLVVVAPDDERADRACSAMHRLGARHPGRTIVVVPDGAGPSRLDARVSLQGGEAAGH
                     MLWSEEVRLHVGGGAADHLDSLVEPLTLPELPVAVWFVDTLPRPADPVAAAADALIVD
                     ARQIDGTDPQAALAGVAELIRANVVVDLSWVRLTPWREILAALFVGPHAADVHRVTGA
                     EVVGRPGPRHLVGGWLVDRLGLSRQGVRLVPDDHVRITLTTVDDGEATRYLVERRGGE
                     HIVRARISTRHGDQASQVLALPDDSVPWSLGT"
BASE COUNT          565 a         1634 c         1553 g          577 t
ORIGIN      
        1 cctcgagtcc tgccccggcg acctgtccgg catccacagc gtggcgtcgt tcttcgtgag
       61 ccgggtggac accgaggtcg accgccggct gaacgctgtc ggcgacccgg cggccggcct
      121 ggccggcaag gcggcggtgg cccaagcgcg actcgcctac cagctgttcc ggcagcggtt
      181 ctcgggggcg cgctgggagg ccctggccgc ccggggcgcc cacttgcagc gacccctctg
      241 ggcctcgacg tcgaccaaga acccggccta cccggacctg ctctacgtcg acaacctgat
      301 cgggccgcac acggtcaaca ccatgcccga cgccacgatc gacgccttct tggaccacgg
      361 cgcggtggcg cgcaccgtgg accaggacgc cgacgcggcc caggccgacc tggcccggct
      421 ggccaccctg ggcgtcgacc tcggggacgt cgcccgcacc ctggaggagg agggcgtggc
      481 cgcgttctcc aagtccttcg acgagctgat ccaggccctt acggacaagg ccaacagcct
      541 tgggtgaggc cctccgacgg ctgctcgccc gggacgccac gttgtggccg gcgggaaacg
      601 tggcggccaa ccggctcggg tggctggacc tgccccggcg gatggccggg gaagcccggg
      661 agctggccgc cttcgcggca tccgtcgacc agcccaaggt cgtgctgctg ggcatgggcg
      721 ggtcctcgct gggcccggag gtgctccggg ccagccgccc gggaccggga cgccggctcg
      781 tcgtgctcga caccacggac ccgcagacgg tcgccggcgt cgatctgtcc gacgccttct
      841 tcctggtctc ctccaaatcg ggcaccaccc tcgaggtcca ggcccacttc gcccacgcgt
      901 gggcccaggt ccccgacggg tcgcgctacg cggccatcac cgaccccggg acgccgctcg
      961 ccgccctcgc cgcggcccac ggcttccgcc gcaccttcga gaacccgccc gacatcggcg
     1021 gccgctactc ggtgctctcc tacttcgggc tggtcccggc ggccctgctc ggctacgacg
     1081 tggccgagct ctgcgagcgg gccctcggca ccgacctgga ggatgccgcc gagctcggcg
     1141 tcgccatggg cgaggcggcg cgccagggcc gcgacaaggt caccgtcgtg gtcccgaagc
     1201 cgttccgggc cttcgggctg tgggtcgagc agctcatcgc cgagtcgacc ggcaagcgcg
     1261 gcaccggctg cgtgccggtc cccacgaccg aggtggagga gggcccggac cgccacgtcg
     1321 tcgccctcga gatcggcacg gccgacgacc tcgccgcgga gttctaccgg tgggagctgg
     1381 ccaccgccat cgccgggcac gtgctcggca tcgacccgtt cgacgagccc aacgtggcgg
     1441 agtccaaggc caacacccgc caggtgctcg actcactgcc ccttccgcag ctggaccatg
     1501 ccgatcccgc cacggtcagc gactggctgc gccggaccgt gcgccccaac gactacgtct
     1561 ccgtgcaggc ctacctgccc tacggccacg acgacgacct cgaggagctc cggcgcaccc
     1621 tgcgcgacgg gctcggcggc gccgcggtca cggccggcta cggcccgcgg ttcttgcatt
     1681 cgacgggcca gctccacaag ggcggccccg acgaggtggt tgccgtccag ctcgtccggc
     1741 gcacgcacgt gccgtcggtg gccatccccg gctttcccta cgactacgac acgctgatcg
     1801 ccgcacaggc gatcggcgac caccgcagcc tgctcgatca cggccggcgg gtgctgcggg
     1861 tcgcggtgga cagcccgcac gaggtgggct agcggtggcc gcggtcacca ccgacctgcc
     1921 gaacccgctg ctgatgggcc tcgagggcga gcgccgggcc ccgccgtgcg tgctggtcgt
     1981 gttcggcgcc tccggcgacc tggcctcgcg caagctgctg cccgccatcg agcagctgtc
     2041 gcggcatcgc cgcctgccgg ccaacttcgc cgtggtgggg gtggcccgca cggacatgac
     2101 cgacgagcag ttccggcgca tgtgcctcga tgccgtcccc ggcgccggcc cggcgtggga
     2161 cggcctggtc gccggcttcc gctacgtcgc cggcgagtac gccaggggcg agaccttcga
     2221 caccctgagc cgggtcctgg ccgaggtgga ccgcgaccgt ggcacggccg ggaaccgggt
     2281 gcactacctg gcgaccatcc ccgcggtgtt cggcgcggtg gccgacgcgc tggcccatca
     2341 cgggctcaac caggcgggcg aggacggcgg cttcgtgcgc gtcgtggtcg agaagccttt
     2401 cggccgcgac ctggagtcgg cccgggcgct cgacgccgag ctgcacaccg ccttcgacga
     2461 gagccagatc taccggatcg accactacct cgggaaggag acggtccaga acgtcctcgc
     2521 cctccggttc gccaacgcca tcttcgagcc catatggaac cggcgctacg tggagcaggt
     2581 gcagatcacc gtggccgagt gcctcggggt cgagcaccgg ggcggcttct acgagacggc
     2641 cggcgccctc cgggacatcg tccagaacca cgtcctgcag gtcctcgccc tcaccctcct
     2701 cgagccgccg gccacggtag acgcccaggg catccgcgac gagaaggtga aggccctgcg
     2761 cgccgtcaag atcctggacc cgtccgaggt gccggcctac gtggtgcggg gccagtacga
     2821 caccgggtgg atcgaaggcg cggaggtgcc cggctaccgg gccgaggccg acgtggcgcc
     2881 cgacagcgag accgagacgt acctggccat gcggctcgac gtcgacaact ggcgctgggc
     2941 gggggtgccg atctacgccc gcaccggcaa gcggctggcc aagagggtga ccgaggtggc
     3001 catgcagttc cactcggtcc cccacctccc gttcgcctcg aagctgtccg agggcctgat
     3061 gcccaacgcg ctggtcgtgc gcgtccagcc cgacgagggg atcacgctgc gcttcggggc
     3121 caaggtgcca ggccaggagt tccgggtgcg cagcgtgtcg atggacttct cctacggggc
     3181 ggcgttcttg gaggagacgc ccgaggccta cgagaggctc ctgctcgatg ccatggccgg
     3241 cgacccgacg ctgttcatcc ggaccgacga ggtggagcag gcctggcgga tcgccgaccc
     3301 catactcgag gcgttcggcg gccgccagcc gccgctggcg cggtacccgg cgggatcgtg
     3361 ggggccccgg gaggccgacg ccctcatagc ccgggacggc cacgcgtggc ggaccccgta
     3421 gccgcccccc tcgagctcgg gtcgtggtcc gggaccggcg tgcgggtcgg cgacgtggag
     3481 cgggccctcg aggacctccg ccacggcgag cagcgcgccg ccacccgcac cagcgtcgtc
     3541 accctcgtcg tggtcgcccc ggacgacgaa cgggccgacc gggcgtgctc ggccatgcac
     3601 cggctcgggg cccggcaccc gggccgcacc atcgtggtgg tgccggacgg tgcgggcccc
     3661 tcccggctgg acgcccgggt ctcgctgcaa gggggtgagg ccgcggggca catgctgtgg
     3721 tcggaggagg tgcgcctgca cgtcggggga ggggcggccg accacctcga ctcgctggtc
     3781 gagcccttga ccctgcccga gctgccggtg gcagtgtggt tcgtggacac gctgcccagg
     3841 ccggcggacc cggtggccgc ggcggccgac gccctgatcg tcgacgcccg ccagatcgac
     3901 gggaccgatc cccaggcggc gctggcaggg gtggccgagc tgatccgcgc caacgtggtg
     3961 gtcgacctgt cgtgggtgcg actgaccccg tggcgcgaga tcctggccgc cctcttcgtc
     4021 gggccccacg ccgccgatgt ccaccgggtg accggggccg aggtggtcgg ccgccctggg
     4081 ccccgccacc tcgtcggggg ctggctcgtc gaccggctgg ggctgtcccg ccaaggcgtc
     4141 aggctcgtcc ccgacgacca cgtgaggatc accctgacca ccgttgacga cggggaggcc
     4201 acgcgctacc tcgtcgagcg ccgtggcggc gagcacatcg tccgggcccg gatctcgacg
     4261 cgccacggcg accaggcgtc gcaggtgctg gcccttcccg acgactccgt gccctggtcc
     4321 ctcggcacg
//
LOCUS       DASWGG010000038         3556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_681908, whole genome shotgun sequence.
ACCESSION   DASWGG010000038 DASWGG010000000
VERSION     DASWGG010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681908"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VF954_00630"
     CDS             complement(<1..244)
                     /locus_tag="VF954_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX9529609.1"
                     /translation="MPASGASVTDRHSPGHAEVAADVGADRRYLLVALGLIAGFMAAE
                     VAVSVVSGSLALLADAGHMLTDAMAIGGSIWALSLAQ"
     gene            complement(220..357)
                     /locus_tag="VF954_00635"
     CDS             complement(220..357)
                     /locus_tag="VF954_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529610.1"
                     /translation="MHLVDQGATWDPFPPTGDLDRGMLAAAEDAALLAPVPRCPRAAP
                     R"
     gene            575..1039
                     /locus_tag="VF954_00640"
     CDS             575..1039
                     /locus_tag="VF954_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9529611.1"
                     /translation="MATVKPIPDGYPRVIPYLTVEGAAAAIDFYKEVFGATERMRMPG
                     PDGKLGHAELQIGDSVLMLADAYPEMGNPPPKALGGTPVTVMVYVEDVDATFERALKA
                     GATEERKVEDQFYGDRAGQLVDPFGHKWFVATHVEDVPPEEMAKRATAAMGG"
     gene            1036..2157
                     /locus_tag="VF954_00645"
     CDS             1036..2157
                     /locus_tag="VF954_00645"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9529612.1"
                     /translation="MTPPSVDPGPRPSVVVIGGGYAGVNAAKALDEAADVVLVDPKDA
                     FVHNIGALRALVEPSFLPRIFLPYDQLLARGRVARDRAVEVTARRVVLASGEAIAADY
                     IVLATGSRYPFPAKTDATNATEAIDDYRAAYGDLARAARVLLVGAGPVGIELAGEITS
                     KWPDKRVTVLDLVGDLLGGQYRPDLRAELRGQLVDLGVELVLGEGLRQFPPTSANELA
                     TFTVTTDSGRQITADIWFQCFGVSPVSDYLGDDLAAARRPDGSVTVGPTLQVAGHDNV
                     FAIGDVSAFDAKMAGVAGRQAKVVADNIIKMANGEEPTDYQPIGPAIMVPVGPNGGSG
                     LLPGEEELVSRERVAAMKGRDLMIDRYAGILGLTTTPPG"
     gene            2185..2685
                     /locus_tag="VF954_00650"
     CDS             2185..2685
                     /locus_tag="VF954_00650"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HEX9529613.1"
                     /translation="MAQINQGRFTADLDGDEIVVFLIGMRVNKPWKVRKWLPVFLAMP
                     KMLRYLTEHPEKGLLGYRQGLTVVVQYWRSFEDLDRFARNGDDPHLEPWRKFVRAVGS
                     SGDVGIWHETFRAKVAEIETIYTNMPTAGLAKFTGGAVPVGRKGNSAAKRIGFRDTDD
                     VAVPAP"
     gene            2752..>3556
                     /locus_tag="VF954_00655"
     CDS             2752..>3556
                     /locus_tag="VF954_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX9529614.1"
                     /translation="MPIARLLRPVRAVAVAVALVGGLVSAGLAATPTAATTSTPALAV
                     TVVGNHLVDGAGNVVRLLGVDRSGTEYACAQGWGIFDGPSDATSIAAMAAWHVNAVRV
                     PLNEDCWLGINHLNASFSGDAYRSAIANYVSALHAAGVVAILDLHWNAPGASVASSQQ
                     VMADADHSPAFWASVASSFRNDRAVAFDLYNEPHDISWSCWRDGCTTAAGWQAAGMQS
                     LVNAVRSTGATQPVLLGGLGWASDLSSWRAWQPNDPAGHAAASFHVYNFS"
BASE COUNT          552 a         1257 c         1225 g          522 t
ORIGIN      
        1 gctgggccag gctcagggcc cagatcgacc cgccgatggc catggcgtcg gtgagcatgt
       61 ggccggcgtc ggccagcaac gccagtgagc cggagacgac cgagactgcg acctctgcgg
      121 ccatgaagcc cgcgatcagc cccagcgcca cgagcaggta ccgccggtcg gcgcctacgt
      181 cggcggccac ttcggcgtgc ccggggctgt ggcggtcggt caccgaggcg ccgctcgcgg
      241 gcaccgcggc acgggggcca gcagggcggc gtcctcggcg gcggcgagca tcccgcggtc
      301 gagatcaccg gtgggcggga acgggtccca cgtggcgccc tgatcgacaa gatgcatgtg
      361 ggatgcctca atccgtgcca agtgggtagc caattttacg ttgcggccga aagtcgggca
      421 acaccggcgc cgccatcgcg gccggcgccg actgccgact tttacgggcc ggcgcggaac
      481 cggatcgagg gggacggccg tcgatgggtg gcagcgagcg aggcgcctcg tgggtgacta
      541 ggttggcgat gcgacctgac gaaggacggt gtccatggca acggtcaagc ccatccccga
      601 cggttacccg cgggtcatcc cctacctgac cgtcgagggg gcggcggcgg cgatcgattt
      661 ctacaaggag gtgttcggcg ccaccgagcg gatgcgaatg cctggcccgg acggcaagct
      721 cggccacgcc gagctgcaga tcggtgattc ggtgttgatg ctggccgacg cgtaccccga
      781 gatgggcaac cccccaccga aggcgctggg tggcaccccg gtgacggtga tggtctacgt
      841 cgaggacgtc gacgccacgt tcgaacgggc gctcaaggca ggcgccaccg aggagcgcaa
      901 ggtcgaggac cagttctacg gcgaccgggc cggccagctc gtggacccat tcggccacaa
      961 atggttcgtc gccacccacg tggaggacgt gccacccgag gagatggcca agcgggccac
     1021 cgccgccatg ggcggttgac cccgccgtcg gtggatccgg ggccgcggcc ctcggtcgtc
     1081 gtgatcggcg gcggctacgc cggagtgaac gcggccaagg cgctggacga ggccgccgac
     1141 gtggtgctcg tggatcccaa ggacgccttc gtgcacaaca tcggcgctct ccgggccctc
     1201 gtggagccga gcttcctccc ccggatcttc ctgccgtacg accagctcct ggcccgcggg
     1261 cgggtggcgc gcgaccgggc ggtggaggtc accgcacgtc gggtcgtcct ggcgtcgggc
     1321 gaggccatcg ctgccgacta catcgtgctc gccacggggt cgaggtaccc cttccccgcc
     1381 aagaccgacg ccaccaacgc cacggaagcg atcgacgact accgggccgc gtacggagat
     1441 ctggcccggg ccgcccgggt cctgctcgtc ggggccggcc cggtcgggat cgagctcgcc
     1501 ggcgagatca cctcgaaatg gccggacaag cgggtcacgg tcctggacct ggtcggcgat
     1561 ctgctcggcg ggcagtaccg ccccgacctg cgcgccgagc ttcgagggca actcgtcgat
     1621 ctcggagtcg agctggtcct cggcgaggga ctgcggcagt tcccgccgac gtcggccaac
     1681 gagctggcca ccttcacggt gaccacggac tcgggccgcc agataaccgc cgacatctgg
     1741 ttccagtgct tcggcgtcag cccggtcagc gactacctcg gcgacgatct ggcggcggcg
     1801 cggcgccccg acgggtccgt gacggtcggg ccgaccctac aggtcgcggg ccacgacaac
     1861 gtgttcgcga tcggcgacgt ctcggccttc gacgccaaga tggcgggcgt cgccgggagg
     1921 caggccaagg tcgtcgccga caacatcatc aagatggcca atggcgagga gcccaccgac
     1981 taccaaccga tcggaccggc gatcatggtg ccggtcgggc ccaacggcgg cagcggccta
     2041 cttcctggcg aggaagagct cgtctcccgg gagagggtcg cggccatgaa ggggcgcgac
     2101 ctcatgatcg atcgctacgc cgggatcctg ggcctcacca ccacccctcc cggctgacgg
     2161 ccggcgggcg cggcacacta cgggatggct caaatcaacc aaggacggtt caccgcggat
     2221 cttgacggcg acgagatcgt ggtgttcttg atcggaatgc gtgtcaacaa gccgtggaag
     2281 gtccgaaagt ggctgccggt gttcctggcg atgcccaaga tgctgcgcta cctgacggag
     2341 catcccgaga agggacttct gggctaccgc caagggctga cggtggtggt ccagtactgg
     2401 cgaagcttcg aggacctcga ccgctttgct cgcaacggcg acgaccccca tctcgagccg
     2461 tggcggaagt tcgtgcgcgc cgtaggcagc tccggcgacg tcgggatctg gcacgagacg
     2521 ttccgggcca aggtcgccga aatcgagacg atctacacca acatgcccac cgccggcttg
     2581 gcgaagttca ccggcggggc cgtcccagtt ggtcgcaagg gcaactcggc cgccaagcgc
     2641 atcggcttcc gcgacaccga cgacgtggcc gttccagctc catagccgag gccttggccc
     2701 caccgcgcac ttaccgattc accggctcgg ctcgatgacc gatagaggag ggtgccgatc
     2761 gcccgcctcc ttcgtcccgt acgcgccgtc gccgtcgccg tcgccctggt cggtggcctg
     2821 gtctctgccg gccttgccgc gacaccaact gcggctacga cctccacccc tgccctggcg
     2881 gtgacggtcg tcggcaacca cctggtcgac ggcgccggca acgtcgtccg gctcctcggt
     2941 gtcgatcgct ccggcaccga gtacgcctgc gcccagggat ggggcatctt cgacggaccg
     3001 tccgacgcaa cgtcgatcgc ggccatggcg gcctggcacg tcaacgccgt gcgagtcccg
     3061 ctgaacgagg actgctggct gggcatcaac cacctcaacg cctccttctc gggggacgcc
     3121 taccgcagcg ccatcgccaa ctatgtctcg gccctccacg ccgcgggcgt ggtcgccatc
     3181 ctcgacctcc actggaacgc cccgggcgct tcggtcgcct cgagccagca ggtcatggcc
     3241 gacgccgacc actctcccgc gttctgggcg tcggtggcgt catccttccg caacgaccgc
     3301 gccgtcgcgt tcgacctcta caacgagccc cacgacatct cctggtcgtg ctggcgcgac
     3361 ggctgcacga ccgccgccgg ctggcaggct gcgggcatgc agtccctcgt caacgccgtg
     3421 cgctcgaccg gcgcgaccca acccgtgctg ctcggtgggc tcggatgggc cagcgacctg
     3481 tcctcgtggc gggcctggca acccaacgac cctgccggtc atgcggcggc cagcttccac
     3541 gtgtacaact tctccg
//
LOCUS       DASWGG010000039         4070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_19353, whole genome shotgun sequence.
ACCESSION   DASWGG010000039 DASWGG010000000
VERSION     DASWGG010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19353"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /gene="nudC"
                     /locus_tag="VF954_00660"
     CDS             complement(<1..828)
                     /gene="nudC"
                     /locus_tag="VF954_00660"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX9529615.1"
                     /translation="MPAGRIPLTGAGADRAAHRRRDTAWLAEASARAESQVLLVRPPA
                     GLISVGRPPSLSFLAVGEVADVREHLILLSVDEDGAATFALDADLEAVAGLEAVARLA
                     GDGPDAAQWGDLRAIGAAIDAAAGNLLAQAAGLVHWHRRHSRCGACGEPTTPGEAGYV
                     RSCNACGLEHFPRTDPAVIMLVVSGDRCILGRQPRWPARMFSALAGFVEPGESLEDAV
                     SREVLEEVGIRVGRVRYLASQPWPFPSSLMLGFRAEAATDELRVDMTELAEAAWWERG
                     "
     gene            984..1166
                     /locus_tag="VF954_00665"
     CDS             984..1166
                     /locus_tag="VF954_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529616.1"
                     /translation="MAEGTGEEAGQVVACPSCGQEVKLHSMIPVLAQAGGIAYLCAAC
                     ARRGVVTAAATAEPSA"
     gene            complement(1266..1991)
                     /locus_tag="VF954_00670"
     CDS             complement(1266..1991)
                     /locus_tag="VF954_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HEX9529617.1"
                     /translation="MPTTTDWAALASRLDAALHLEEPPIAITFGPAPPGVDAFDEPFA
                     PPAEDGRTGRVPAGCVFWMRAADRTFTTVAEDHGNCSVGMMTHGFRTLEQVAGNSDVA
                     TLVGSGWVTEDMVPGIPTVARPAEAVTYGPLASTPVDPDVVLLRLNAKQAMVLSDALP
                     GLRVEGKPQCHIVAVAKEQGEVAASVGCMLSRVRTGLPSTQLTCAVPAAKLADVTEAV
                     GRTAAIDAVVGSYAAEDARRFTG"
     gene            2403..3359
                     /locus_tag="VF954_00675"
     CDS             2403..3359
                     /locus_tag="VF954_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9529618.1"
                     /translation="MPSTRSRQSRHDSDTEQDLVRLYLDDIGRHPLLTKGDEVRLAQA
                     IEAGRIARASLASGEKFERARKRALQRELRGGEDATQTFINANLRLVVSIAKKYQSSG
                     LPLLDLVQEGNLGLMHAVEKFEWRKGFKFSTYATWWIRQAIQRGIANSGRTIRLPVHA
                     VDQVAKVRKVGDRLEGELGRAPRMAELAEAMEMTEAQVADILKFGSEPRSLHERLSED
                     SDTELGDMVEDANATTPLDLVLAGAMSGEVEKLLSRLDAREQQVLRLRFGLDRGEPRT
                     LDEVGEHIGLTRERARQIEARALSKLRHPCNEGGAAELLVAS"
     gene            complement(3384..3800)
                     /locus_tag="VF954_00680"
     CDS             complement(3384..3800)
                     /locus_tag="VF954_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9529619.1"
                     /translation="MLSADDRLDIIDLLAATAQAIDSRDGDALITMFTPDGQYTTGQE
                     RADTAADIASMASRQSRTGTIRRHTQTTTIEETGEGCAHAVSYVLVTEVQPTGSGVEL
                     VNVASGVYDDDVILTEQGWRIAARFGAADGEWSEMI"
     gene            complement(3880..>4070)
                     /locus_tag="VF954_00685"
     CDS             complement(3880..>4070)
                     /locus_tag="VF954_00685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX9529620.1"
                     /translation="VGVAVANLSHDRAAQLELPFDRHGLDLDAALDGIRDRFGPAGVT
                     RARLLGRDQGPTVPLLAD"
BASE COUNT          593 a         1458 c         1465 g          554 t
ORIGIN      
        1 cccgcgctcc caccaggccg cctcggccag ctcggtcatg tccacccgca gctcgtccgt
       61 agccgcctcg gcccggaacc cgagcatcag cgacgacggg aacggccagg gctgggaggc
      121 caggtagcga acacggccga cgcggatccc gacctcttcc agaacctctc tgctcaccgc
      181 gtcctccagc gactcgccgg gctccacgaa cccggccagg gccgagaaca tccgggccgg
      241 ccacctcggc tggcgcccga ggatgcagcg gtcgcccgac accacgagca tgatcacggc
      301 cgggtccgtg cgggggaagt gctcgaggcc acaggcgttg cagctgcgca cgtagccggc
      361 ctcgccgggc gtggtgggct cgccgcaggc gccgcagcgg ctgtggcggc ggtgccagtg
      421 gaccaggccg gcggcctggg cgaggaggtt gccggccgcg gcgtcgatgg cggcgccgat
      481 ggcgcgcagg tcgccccact gggcggcgtc ggggccgtcg cctgccagcc gggcgacggc
      541 ctccagcccg gcgacggcct ccaggtcggc gtccagagcg aaggtggcgg cgccgtcctc
      601 gtcgacggag agcaggatga ggtgctcgcg cacgtcggcc acctcgccga cggccaggaa
      661 cgacagactg ggcggccggc ccacgctgat gagcccggcg ggcggccgca cgagcagcac
      721 ctgcgattcg gcccgggcag aggcctcggc cagccaggcg gtgtcccgcc tgcggtgggc
      781 ggcgcggtcg gcgcccgcac cggtgagggg gatgcggccg gcgggcatcg gtcccgaacc
      841 ctaccgggaa tgccttgtcg ccaacagcgt ccgtcccgtg gtcaccgtcg tcacggggca
      901 gccgacggcg cgggtcgtcg ccgggccgtt aggctgctgg cgcctctggc tctcggacgg
      961 gggcgagcga aggagagctc cagatggccg aaggcaccgg agaggaagcc ggccaggtcg
     1021 tggcctgccc gtcgtgcggg caggaggtga agctgcactc catgatcccg gtgctcgccc
     1081 aggccggcgg catcgcctac ctgtgcgcgg cgtgcgcccg gcgtggggtc gtcaccgccg
     1141 cggcaaccgc cgaaccctcg gcatagcacc tcgcatctaa ccattcttaa aggcttttat
     1201 gtcgctccag tgtgttttct gcttgaagaa tggaatcgcc gtgcgccgcc tcgccgtgcg
     1261 ctggctcagc ccgtgaagcg ccgggcatcc tccgccgcgt agctgcccac cacggcgtcg
     1321 atcgcagccg tgcgccccac cgcctcggtg acgtcggcca gcttggccgc cgggacggcg
     1381 caggtgagct gggtgctggg caggcccgtg cggacccggg acagcatgca acccacgctc
     1441 gcggccacct cgccctgttc cttggccacg gccacgatat ggcactgcgg cttgccttcg
     1501 acccgcaagc cgggcagggc atcggacagc accatggcct gtttggcgtt caggcgcagc
     1561 agcaccacgt cggggtcgac gggggtggac gccagaggtc cgtaggtcac ggcttccgcc
     1621 gggcgggcga ccgtggggat gcccggcacc atgtcttcgg tgacccatcc cgagcccacc
     1681 agcgtggcga cgtcggaatt gcccgccacc tgctccaggg tccgaaaccc gtgggtcatc
     1741 atgcccacgc tgcagttgcc gtggtcctcg gcgacggtgg tgaaggtgcg atcggcggct
     1801 ctcatccaga agacgcaccc ggcaggcacc cggccggtcc ggccgtcttc cgccggcgga
     1861 gcgaagggct cgtcgaaggc gtccaccccc gggggagcag gcccgaaggt gatggcgatc
     1921 ggcggctcct ccaggtgcaa ggcggcgtcg agcctgctgg ccagagcggc ccaatcggtc
     1981 gtggtcggca tgggggcatc tcctctgggg ccggggcgga atggcggacg gcggcgggtg
     2041 cgttcacctg gccgcgggac gtctcgcttg gcgcgacctg ttgggcaagg tagccgccat
     2101 cacggacggg ctccaccccc gacccgccac atggcccggc cctccaccgc gggatctcgg
     2161 ctgccattgc ctggcttgag gcccgttccg gcggtaagct gaacgcggct gttgcggcca
     2221 ggttcctggg cgcacccacg gagagtggtg ttcctctctt cgatcgtcac tgaacgcggc
     2281 tgcaaggggc agccccgagg ggtgcgaacg ggcgaaccgg gaacgacaag tggccagcaa
     2341 ccgctctcaa accggtcggg cggatcgccc ggcagaccca agaaggacgt tccaagactt
     2401 ccgtgcccag cacccgatcc cgacagagcc gacacgacag cgacacggag caggacctgg
     2461 tccggctcta tctcgacgac atcggccgac atcccctcct caccaagggc gacgaggtgc
     2521 gcctggccca ggctatcgaa gccggccgaa tcgcccgcgc cagcctggcc agcggggaga
     2581 agttcgagcg ggcccgcaag cgggcgctcc agcgggagct gcggggcggc gaggacgcca
     2641 cccagacctt catcaacgcc aacctgcggc tggtggtctc gatcgccaag aagtaccagt
     2701 cgtccgggct tccgctcctc gacctcgtcc aggagggcaa cctgggcctc atgcatgccg
     2761 tggagaaatt cgagtggcgc aagggcttca agttctccac ctacgccacc tggtggatcc
     2821 gccaggccat ccagcggggg atcgccaaca gcggccgcac catccgactg cccgtgcacg
     2881 cggtggacca ggtcgccaag gtgcgcaagg tcggcgaccg gctggagggc gagctgggcc
     2941 gcgccccccg gatggccgag ctcgccgagg ccatggagat gaccgaagcc caggttgccg
     3001 acatcttgaa gttcggctcc gagccccgca gcctccacga gcgtctgtcc gaggactccg
     3061 acacagagct cggggacatg gtcgaggacg ccaacgccac cacgcccctc gacctggtgc
     3121 tcgccggggc gatgtcgggc gaggtcgaaa agctgctctc gcgcctcgac gccagggagc
     3181 agcaggtcct gcggttgcgc ttcggcctcg accggggcga gccccgcacc ctcgacgagg
     3241 tcggcgagca catcggcctg acccgggagc gggcccgcca gatcgaggcc cgggccctct
     3301 ccaagctccg ccacccttgc aatgagggcg gggcggcgga gctgctggtc gccagctgac
     3361 cgccgggcgg gcggcccggc acctcagatc atctcgctcc actcaccgtc cgcggctccg
     3421 aagcgcgccg cgatgcgcca accctgctcg gtgaggatca cgtcgtcgtc gtagacaccg
     3481 ctcgccacgt tcaccagctc gacgccgctg ccggtcggct gcacctcggt caccaggacg
     3541 taggacacgg catgggcgca accctcgccg gtctcctcga tggtcgtcgt ctgggtgtgc
     3601 cgccggatgg tccccgtccg gctctgcctg ctcgccatgc tcgcgatgtc cgcggcggta
     3661 tcggcccgct cctggccggt tgtgtactgg ccgtccgggg tgaacatggt gatcagggca
     3721 tcgccatcgc gactgtcgat ggcctgggcg gtggcggcca ggagatcgat aatgtcgagg
     3781 cggtcgtcgg cgctgagcat cagcgtcgaa gggtaggccc ttggcgtggc cgcgcgcctg
     3841 ggccgggggc gcaccctggg gcccggcgag aacgaccggt caatcggcca ggagcgggac
     3901 ggtagggccc tggtcgcggc cgagcagtcg cgcccgggtc acccccgccg ggccgaagcg
     3961 gtcccggatg ccgtccagcg ccgcgtccag gtcgaggccg tggcgatcga acggcagctc
     4021 gagctgcgcg gcccggtcgt ggctcaggtt ggcgaccgcc acgccgacca
//
LOCUS       DASWGG010000040         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1652432, whole genome shotgun sequence.
ACCESSION   DASWGG010000040 DASWGG010000000
VERSION     DASWGG010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652432"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VF954_00690"
     CDS             <1..590
                     /locus_tag="VF954_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9529621.1"
                     /translation="TMHGIRRLQRDKVVTHAGVSNFDLARWEAAEAALGSAVLSNQVL
                     FNLVHRRPEHDMLPWAQETGHVVIAYSPLAQGFLGARYDADNRPGGVRATNALFLPEN
                     LRRGQEVLAALREVAAAHGVTPAQVALAWLIRKPNVVAIPGASSVAQLEANAAAAEIT
                     LTEDENLRLTAAAEAFEPVSGPSALPRLVGARFRR"
     gene            613..1677
                     /gene="galE"
                     /locus_tag="VF954_00695"
     CDS             613..1677
                     /gene="galE"
                     /locus_tag="VF954_00695"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX9529622.1"
                     /translation="MSSTVLVTGGAGYIGSHTCLELLGAGHRVVVVDNLSNSSPAAIE
                     RVRELAGPGAPELVFVEADLRDTAVLERVVGGAGASSVVHFAGKKAVGESVAQPLDYY
                     DNNFVGTLNLLRAMEGAGVRRLVFSSSCTVYGEPSELPLTEAASRWAFSPYGRTKLFV
                     EEMIQDLVTAQPEAGWRVMLLRYFNPVGAHPSGRIGEDPEGIPNNLMPFVMQVSVGLR
                     DAVRVFGDDYDTVDGTGVRDYVHVMDLAAGHLAALSALERNAGPSSSGACTAVNLGTG
                     LGHSVLEVIAAASRAVGRDVPYRVTARRPGDVACVYADPRLAADLLGWRATRSLDDMC
                     RDHWAWQRANPSGYRVDAGR"
     gene            1835..>2232
                     /locus_tag="VF954_00700"
     CDS             1835..>2232
                     /locus_tag="VF954_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529623.1"
                     /translation="MAVGSRGGRRHVGALRALAAGAAAFSVLALGISWVATRATVAPS
                     SSPAATSSGPPAAIVGMAATPDGGGYWLVGPDGGVLSFGEARFFGSTGDTKLRSPVVG
                     VVSAPDGGGYWLAARDGGVFAFGGARFFGS"
BASE COUNT          286 a          831 c          784 g          331 t
ORIGIN      
        1 cgacgatgca cggcatccgc cgcctccaac gagacaaggt ggtcacccac gcgggcgtct
       61 ccaacttcga cctggcccgc tgggaggccg ccgaggccgc cctgggctcg gcggtgctga
      121 gcaatcaggt gctcttcaac ctggtccacc ggcggcccga gcacgacatg ctgccctggg
      181 cccaggagac cggccacgtc gtgatcgcct acagcccgct ggcccagggg ttcctcgggg
      241 ctcggtacga cgccgacaac cggccagggg gcgtgcgcgc caccaacgcc ctctttctcc
      301 ccgagaacct gcggcgaggt caggaggtgc tcgccgccct ccgggaggtc gccgcggccc
      361 acggcgtcac gccggcgcag gtcgccctgg cctggctcat ccgcaagccc aacgtggtcg
      421 ccatccccgg ggcgagcagc gtcgcccagc tggaagccaa cgccgccgcc gccgagatca
      481 cgctcacaga ggacgagaac ctccgcctca ccgccgccgc cgaggcgttc gagcccgtca
      541 gcggcccctc tgccctcccc agactggtcg gtgcccgctt ccgccgttga gcccgccgct
      601 accgtggccg ccatgtcgtc aaccgtgctt gtcaccggcg gcgccggcta catcggctcc
      661 cacacctgcc tggagctgct gggggccggg caccgggtgg tcgtggtcga caacctgtcc
      721 aacagctcgc ccgcggccat cgaacgggtc cgcgagctgg ccggcccagg agccccggag
      781 ctggtcttcg tcgaggccga cctgcgggac accgcggtcc tcgaacgggt cgtgggcggg
      841 gctggcgcct cttctgtcgt gcacttcgcc gggaagaagg ccgtcggcga gtcggtcgcc
      901 caacccctcg actactacga caacaacttc gtcgggaccc tcaacctgct ccgtgccatg
      961 gagggcgccg gcgtacgccg gctggtcttc tcctcctcct gcaccgtgta cggcgagccg
     1021 tcggagctgc ccctgaccga ggcggcgagc cggtgggcgt tcagcccgta cgggcgcacc
     1081 aagctcttcg tcgaggagat gatccaggat ctcgtgaccg cccagcccga agcgggttgg
     1141 cgggtgatgc tgttgcgcta cttcaacccc gtcggcgctc acccgagcgg gcggatcggc
     1201 gaggacccgg aaggcatccc caacaacctg atgcccttcg tcatgcaggt ctcggtcggg
     1261 ttgcgtgacg cggtgcgggt cttcggcgac gactacgaca ccgtggacgg aaccggcgtg
     1321 cgggactacg tccacgtcat ggacctggcc gccggtcacc tggccgcgct gagcgccctc
     1381 gagcgcaacg ccggccccag ctcctcgggg gcgtgcacgg cggtcaacct cggcaccggc
     1441 ctgggccact cggtgctcga ggtcatcgcc gccgcgtccc gggcagtcgg ccgcgacgtg
     1501 ccctaccggg tgacggcccg caggccgggg gacgtggcgt gcgtgtacgc cgatccgcgg
     1561 ctggccgccg atctgctcgg ctggcgggcg acccgctcgc tggacgacat gtgccgggac
     1621 cactgggcct ggcagcgggc caatcccagc ggctaccggg tcgacgcggg caggtagacc
     1681 agcagctcac cgacccggcc gggcgccttc ctcggggacc tcccgcacca cggggtctcg
     1741 ggcccgagcc ctcgacatcg cgtaccatat gcccgtgcca tagcgaggtc tctccgcgcc
     1801 gcggccggga ctggacggtt cgaaaggttt ggccatggcg gtgggctccc gaggtgggcg
     1861 acgtcacgtt ggcgcgcttc gcgccctcgc ggccggcgcg gctgcgttct cggtcctcgc
     1921 gctgggcatc tcctgggtgg cgacgcgggc gacggttgcc ccgtcgagct ccccggccgc
     1981 caccagctcg ggacctcccg cggccatcgt cggcatggct gccacacccg acggcggcgg
     2041 ctactggctc gtgggccccg acggtggagt gctcagcttc ggcgaagcac gcttcttcgg
     2101 ctcgacgggt gacaccaagc ttcggtcgcc ggtggtaggc gtggtgagcg cacccgatgg
     2161 cggtgggtac tggctggccg cccgggacgg aggggtgttc gccttcggcg gggcgcgctt
     2221 cttcggctcg at
//
LOCUS       DASWGG010000041         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1008340, whole genome shotgun sequence.
ACCESSION   DASWGG010000041 DASWGG010000000
VERSION     DASWGG010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008340"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VF954_00705"
     CDS             complement(<1..1100)
                     /locus_tag="VF954_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9529624.1"
                     /translation="MAVLTGVVVAGAGARGAYEAGALSVLLPELERQEPDQRFVVVGT
                     SAGAINAAVLASTESPKEGADALTEFWDDFQVDDVIGNLAATGAGDALKYLFEVVCHW
                     SRLDSLLDSTPLRKTLADRIQWDAVDRNVGRGWIDRVGIVCTCCDNGRTTVFTKGAPP
                     TPADNLRGIDYVPVTLGPEHVSASTALPIVFRPAPIEGPGGGRRWYVDGGVNLNAPIK
                     PAIDLGADRVAVVATNPDPDRPQSAPFGRGEPDVFDIGGAVVRSLLVDNMSEDVRTLR
                     KVNRLVVAAGAAQPRPAGAATSRQYTEIPSLYIGPPGPDDISRLANRVYGDRYDSVLK
                     LTTKLGLLGRVIGGSADSHGDLMSFLFVDKLF"
     gene            complement(1120..2706)
                     /locus_tag="VF954_00710"
     CDS             complement(1120..2706)
                     /locus_tag="VF954_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9529625.1"
                     /translation="MTMQAAAAAAAHGAGDASLYGIQLTVRPGDGGPEDLAVGTARPG
                     VAMTADTLLLWLSVSKPVVAVCVAMLWERELLGLDEPVWHHLPELRRPPLDQLTVRHL
                     LTHTAGFDDDPPPGAMALEWDEAVTLACRSRLEDGWLPGRMAKYSTWTGFLLLAEMVR
                     RVDGRAFSVFAREEVFRPLGMDDCGFSWPEHDLPDPSRLADLSYPTARGPLPDPVLND
                     RGLLVRCWPALGLRGPASQVARFYCSLLPDPGELPPLLRRPTIEAITSRHHVGIRYHR
                     FLKDVCVGLGTLVGAEMFGPYCSSRTFGGRGHGSSVAFADPVHNLVVVAGWTMLLQDD
                     TKHGSRVKALSGAVYEDLGLNVRVRRAVAAAPATPSPDRTGGGGGVEHDGRAQGDGPL
                     LDALRGRSDAEMASIVSGAGGPDALLDPVRTALAEAIRPSAVQPAVVGLALRWPDGER
                     HSTIRITPGVAPTVVAGDAGDAELTIGSTGFDMIRLLARELDIKEALTTGRLRIDGEL
                     AVISAWQRMFGGSVFAMGLS"
     gene            complement(2703..>4175)
                     /locus_tag="VF954_00715"
     CDS             complement(2703..>4175)
                     /locus_tag="VF954_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX9529626.1"
                     /translation="VPVSSTKSMIGHTLGAAGAIEAAACVLSLDRGVVPPTVNLPVER
                     DELFDFVPDHGREQDVHLVMSNNYAFGGSNASVVFARPGAPAPARPTPEVGVSITGVG
                     VIGSLGATHQEWVSALGGGRSGIAPIAAFDASRFRCRQAAEGRKLPTRGFAAPNAWRK
                     MDTFEKGCVASARMAWNDAGIEPTPEEAEGVAVIFATSNGSFETMTTFERGARISAGE
                     ANPSLFPHTAMNAPAGHVCSTLGLRGPTSTITAGGVSGLSALVYGVDLIRQGEASTCL
                     IVAADQFCEAALEMFDPWDGVLTHDTVRPFDRRANGTALGEAAVTFVLERSDLASERG
                     RRSYGRVLGRGMTGDCASLGGISATADEWHQAMALALASSDLDPGDLDYCAAAASGIA
                     PVDAAEAVALSRLVGDTVAVGAPKSVTGECMAASGAINVVACLAAINDGVLTPSVGLD
                     DPIPGSLRHVTGLARGDARHCLANSMSFGGNYVSVVIGAE"
BASE COUNT          607 a         1507 c         1504 g          557 t
ORIGIN      
        1 tggaacagct tgtcgacgaa caggaagctc atcaggtcgc cgtggctgtc cgccgatcct
       61 cctatgaccc gaccgagaag gccgagcttg gttgtcagct tgaggaccga gtcgtagcgg
      121 tccccgtaga cgcggttggc caggcggctg atgtcgtcgg gaccgggagg accgatgtac
      181 aggctcggga tctcggtgta ctgccgggac gtggccgcgc cggccggccg cggctgggcc
      241 gccccggccg cgacgaccag gcggttgacc ttgcgcaggg tgcggacgtc ctcgctcatg
      301 ttgtcgacga ggagcgaccg cacgacggcg ccgccgatgt cgaacacgtc gggctcgccg
      361 cgtccgaagg gtgcggactg cgggcggtcc gggtcggggt tggtggccac gacggcgacc
      421 cggtccgccc cgaggtcgat cgccggcttg atcggcgcgt tgaggttgac ccctccgtcg
      481 acgtaccatc ggcgcccccc accggggccc tcgatggggg ccgggcggaa cacgatcggc
      541 agcgcagtcg acgcgctgac gtgctcgggc ccgagggtca ccgggacgta gtcgatgccg
      601 cgcaggttgt ccgccggggt cgggggagcg cccttggtga acacggtggt ccgaccgttg
      661 tcgcagcagg tgcagacgat cccgacccgg tcgatccagc cgcggccgac gttgcgatcg
      721 accgcgtccc actggatcct gtccgcgagc gtcttcctga gcggagtcga gtcgaggagg
      781 ctgtcgaggc gcgaccagtg gcagaccacc tcgaagaggt acttcagggc gtccccggcg
      841 ccggtcgcgg ccaggttccc gatcacgtcg tcgacctgga agtcgtccca gaactccgtc
      901 agggcgtctg cgccctcctt cggcgactcc gtgctggcca gcacggcggc gttgatcgcg
      961 ccggcgctgg tgccgaccac cacgaagcgc tggtcgggct cctggcgctc gagctccggc
     1021 agcaacaccg acagggcccc ggcctcgtag gcgccgcggg cgccggcccc ggcgacgacg
     1081 acgccggtca gcacggccat ggccccaccc aggtcgctgt cacgacaggc ccatggcgaa
     1141 cacgctgccc ccgaacatcc gctgccaggc gctgatcacg gccagctcgc cgtcgatcct
     1201 gagccgcccg gtcgtcagag cttccttgat gtccagctcc cgggccagga gccggatcat
     1261 gtcgaatccg gtggagccga tggtgagctc ggcgtccccg gcgtccccgg cgacgacggt
     1321 cggtgccacg cccggggtta tcctgatggt gctgtggcgc tcaccgtcgg gccaacggag
     1381 agcgaggccg accaccgccg gctgcaccgc cgacggccgg atggcctcag ccagggcggt
     1441 ccggaccggg tccagcaacg cgtccgggcc gccggcgccg gagacgatgg acgccatctc
     1501 ggcatcggag cggccccgca acgcgtccag cagcgggccg tcgccctggg cgcgtccgtc
     1561 gtgctcgacg cctcccccgc cgccggtcct gtccggtgac ggcgtggcgg gagcggcggc
     1621 caccgctcgc cggaccctga cgttgagccc gaggtcctcg tacacggcgc ccgacaacgc
     1681 cttcacccgg gatccgtgct tggtgtcgtc ctggagaagc atcgtccagc ccgccaccac
     1741 gaccaggttg tggactggat cggcgaaggc gacggaggag ccgtggcccc ggccaccgaa
     1801 ggtccgcgaa gagcagtagg gcccgaacat ctccgccccg acgagggtgc ccaagcccac
     1861 gcacacgtcc ttcaggaagc ggtggtagcg gatccccacg tgatgccgcg aggtgatcgc
     1921 ctcgatggtg ggccggcgca gcagcggcgg cagctccccg gggtcgggca gaagcgagca
     1981 gtagaacctg gcgacctgcg aggccgggcc gcgcaggccc agagccggcc agcatcgcac
     2041 gaggagcccg cgatcgttga ggacgggatc gggtagcgga cctcgggcgg tcgggtagga
     2101 caggtcggcg agccgggacg gatccgggag atcgtgctcc ggccaagaga aaccgcagtc
     2161 gtccatcccc agcgggcgga acacctcctc ccgggcgaac acgctgaagg cgcgcccgtc
     2221 gacgcgccgt accatctcgg ccagcaggag gaagccggtc caggtgctgt atttcgccat
     2281 cctccccggc agccaaccgt cctcgagccg ggaccggcac gccagcgtca ccgcctcgtc
     2341 ccactccagt gccatcgccc ccggcggagg gtcgtcgtcg aatcccgcgg tgtgggtcag
     2401 gaggtggcgc acggtgagct ggtcgagcgg ggggcgccgc agctcgggca ggtggtgcca
     2461 cacgggctcg tcgagcccca gcagctcacg ctcccagagc atggcgacgc atacggcgac
     2521 gacgggcttg ctcaccgaca accacagcag cagggtgtcg gcggtcatgg cgaccccggg
     2581 cctggcggtc ccgacggcga gatcctccgg cccgccatcc cccggccgca cggtgagctg
     2641 gatgccgtag aggctggcgt cgcccgcgcc gtgcgccgcc gccgcggccg cggcctgcat
     2701 cgtcattccg cgccgatcac gacgctcacg tagttgccgc cgaacgacat cgagttggcc
     2761 aggcagtgcc gggcgtcgcc ccgggcgagc ccggtgacat ggcgcaggga cccggggatg
     2821 gggtcgtcca ggccgaccga gggggtcagc acgccgtcgt tgatggccgc gaggcaggcc
     2881 accacgttga tggccccgga cgcggccatg cactcgcctg tcaccgactt cggcgcgccg
     2941 acggcgacgg tgtcaccgac gagccgcgag agcgcaaccg cttcggccgc gtcgacgggg
     3001 gcgatccccg aggcggcggc ggcgcaatag tcgaggtcgc cgggatcgag gtcgctcgac
     3061 gccagcgcca gcgccatggc ctggtgccac tcgtcggccg tggcgctgat cccgcccaac
     3121 gacgcgcagt ccccggtcat accgcgaccg agcacccgcc cataggagcg ccggccgcgc
     3181 tcggaggcga ggtcggagcg ttcgagcacg aaggtcaccg ccgcctcccc cagcgccgtc
     3241 ccgttggccc gccggtcgaa cggccgcacg gtgtcgtggg tcagcacacc gtcccacggg
     3301 tcgaacatct cgagcgcggc ctcacagaac tggtcggccg ccacgatcag gcaggtcgag
     3361 gcctcgccct gacggatcag gtcgaccccg tacaccagag cgctgagtcc ggagacgccc
     3421 ccggcggtga tcgtcgacgt cggcccgcgc agcccgaggg tgctgcacac gtggccggcg
     3481 ggagcgttca tcgccgtgtg ggggaacagg ctcgggttgg cctcgccagc gctgatcctc
     3541 gcgccgcgct cgaaggtagt catggtctcg aaggagccgt tgctcgtggc gaagatcacc
     3601 gcgacgcctt cggcctcctc cggggtgggc tcgatgccgg cgtcgttcca ggccatccgg
     3661 gcgctggcca cgcagccctt ctcgaaggtg tccatcttgc gccaggcgtt cggcgccgcg
     3721 aacccgcggg tcggaagctt gcgcccttcg gcggcctgcc ggcaccggaa gcgagatgcg
     3781 tcgaacgcgg cgatcggagc gatccccgag cgaccgcctc ccagggcgct cacccactcc
     3841 tggtgggtcg cgcccaggct gccgatgaca ccgacgcccg tgatgctcac gccgacctcg
     3901 ggggtcggac gagccggcgc cggcgccccc ggccgggcga agaccaccga cgcgttcgag
     3961 cctccgaacg cgtagttgtt ggacatgacg aggtgcacgt cctgctcgcg gccatggtcg
     4021 gggacgaagt cgaacagctc atcgcgctct accggcaggt tgaccgtcgg cggcacgaca
     4081 ccccggtcca gcgagaggac gcatgcggcc gcctcgatgg ctcccgccgc cccgagcgtg
     4141 tgcccgatca tcgacttcgt gctgctcacg ggcac
//
LOCUS       DASWGG010000042         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2277142, whole genome shotgun sequence.
ACCESSION   DASWGG010000042 DASWGG010000000
VERSION     DASWGG010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2277142"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="VF954_00720"
     CDS             <1..1272
                     /locus_tag="VF954_00720"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX9529627.1"
                     /translation="GLDEPFDCRPDARRVSDAILARSAELNCRLPSRVNIAFGGCSSC
                     RAHARINDAGFESRVVDGQAGYRLWAGGSLGTMPALALEVVGFLPRAEVVAAAEALVD
                     VFVTLGDIAHPKRGRMKFAVERVGADGFRAAFDASFARGRARCEPAPPPVETLGAADF
                     SEALAIVPPGGWAEGARPQRTPGRAMVTVNVPLGDLTSDEMRLLSSLAPTGDGQLHLT
                     RNQNAMFRDVCVADIPGLRLALASHGLSTEGADAAGDVRACTGSAVCALGITAAPAAG
                     ARLIGHSALARNSGLRVHVSGCPNSCAQHQAAAIGLSGATVRINGEIRLGYHVWLGGD
                     LEAGRLGVLAGRVADDSVEEAVGAIVGCWEATRAVGESLPSTVARIGADAFCRHLEAV
                     ASGFQTGAGEAEPPEVSRALPIGGALTGGVR"
     gene            1364..2329
                     /locus_tag="VF954_00725"
     CDS             1364..2329
                     /locus_tag="VF954_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEX9529628.1"
                     /translation="MPVPISETLDDQEAAAAAKARTAKTELPKYLVTSGLAGAYVGVA
                     VVLLLMVSAAFVAGKSPSTKLVQGAVFGIALTLVVFAGAELFTGNVMVMLQGLMRRKV
                     TISDLVLVWAASLLGNLGGSIGFAALVNASGVMSSGAPKGQQTIFMATLAGIVKSKAA
                     LSGGQLFFRSVLCNMLVCLALWMAARTRSDAAKLLCLWWALLAFIASGFEHSIANMTI
                     FGLGIFGHVSGATWAEMSRNLLYTVPGNIVGGGVLVGMVYGWTGREASPSSLGATGTI
                     SVDGPGTIELETAEEGDRPAAVAPRNGAGGRRRATTPPPRRTPVR"
     gene            2405..>2692
                     /locus_tag="VF954_00730"
     CDS             2405..>2692
                     /locus_tag="VF954_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529629.1"
                     /translation="MPMTTTAGDTRANIVAASLVGAVVLVLGFGSGIGAVFSRHTAFK
                     AGAAHGAHGTQAAAVGSPGSVALPAADGSGAAILAAEPVASSRYAAPPVMSA"
BASE COUNT          359 a          998 c          951 g          384 t
ORIGIN      
        1 ggcctggacg agccgttcga ctgccggccg gacgcccggc gcgtatcgga cgccatcctg
       61 gcccgctccg cggagctgaa ctgccggctc ccgagccgcg tcaacatcgc cttcggcggt
      121 tgttcgagct gtcgggccca cgcccggatc aacgacgccg gcttcgagtc gcgggtcgtc
      181 gacggccagg ccggctaccg cctgtgggcc ggaggcagtc tcgggacgat gccggcgctc
      241 gccctcgagg tggtcggttt cctcccgcgg gccgaggtgg tcgcggcggc tgaggccttg
      301 gtcgacgtgt tcgtcaccct cggcgacatc gcccacccca agaggggccg gatgaagttc
      361 gcggtcgagc gggtgggcgc ggacggcttc cgggccgcgt tcgacgcctc gttcgcccgg
      421 gggcgcgcca ggtgcgagcc ggcgccacca cccgtggaga ccctcggggc cgccgatttc
      481 tccgaggccc tggccatcgt gcctcccggc gggtgggcgg aaggggcgcg gccccaacgc
      541 acgccggggc gcgccatggt cacggtcaac gtcccgctgg gcgacctcac cagcgacgag
      601 atgaggctgc tctcgtcgct cgcgccgacg ggtgacggcc agctgcacct gacccgcaac
      661 cagaacgcca tgttccgcga cgtctgcgtc gccgacatcc ccggactgcg cctggcgctc
      721 gcctcccacg ggctgtccac ggaaggggcc gatgcggccg gcgacgtgcg ggcctgcacc
      781 ggctccgccg tctgcgccct cgggatcacg gccgccccgg cggcgggcgc acggctcatc
      841 ggtcattcgg cgctcgcccg caactccgga ctgcgggtgc atgtctcggg ctgcccgaac
      901 tcctgcgccc agcaccaggc cgcagcgatc gggctctcgg gcgccacggt gcggatcaac
      961 ggcgagatcc ggctcggcta ccacgtgtgg ctcgggggcg acctcgaagc cggccgtctc
     1021 ggcgtcctgg ccggacgggt cgccgacgac agtgtcgaag aggcggtggg cgccatcgtg
     1081 ggttgctggg aggcgacgcg ggccgtgggg gagtccctgc cctcgaccgt cgcccggatc
     1141 ggcgcggacg ccttctgccg ccacctggag gcggtcgcct ccggcttcca gaccggtgcc
     1201 ggcgaggccg agccgcccga ggtgagccgg gccctgccga tcggcggagc cctgacgggg
     1261 ggcgtccggt agggcccccc ggcagcacca caccacacca cacccaccac gaccagccca
     1321 cacaagacca gcccacacag gaccagcaca gagaggatca ccgatgcccg taccgatcag
     1381 cgagacgctg gacgaccagg aggcggcggc ggccgccaag gccaggacgg cgaagaccga
     1441 gctgccgaag tacctggtca cctccggctt ggccggcgcc tacgtcggcg ttgccgtcgt
     1501 gctgttgctg atggtcagcg ccgcgttcgt ggccggcaag tctccgagca ccaagctcgt
     1561 ccagggcgcg gtgttcggca tcgccctcac gctcgtggtg ttcgcaggtg ccgagctgtt
     1621 caccggcaac gtcatggtca tgctccaggg gctgatgcga aggaaggtga cgatcagtga
     1681 cctcgtgctc gtatgggcgg cgtcactgct cggcaacctg ggcgggtcca tcggcttcgc
     1741 cgccctcgtc aatgcgagcg gggtgatgag ctccggcgcc cccaagggcc agcagaccat
     1801 cttcatggcc accctcgccg gcatagtgaa gtcgaaggcc gcgctgagcg ggggtcagct
     1861 gttcttccgc tccgtgctgt gcaacatgct cgtctgcctc gccctctgga tggccgcccg
     1921 gaccaggagc gacgcggcca agctcctgtg cctctggtgg gcgctgctgg ccttcatcgc
     1981 ctcgggcttc gagcactcca tcgccaacat gacgatcttc gggctcggca tcttcggcca
     2041 cgtctcgggc gcgacatggg ccgagatgtc ccgcaacctc ttgtacaccg tgcccggcaa
     2101 catcgtgggc ggtggcgtgc tcgtcggcat ggtgtacggc tggacgggac gcgaagcgtc
     2161 tccgagctcg ctgggcgcga ccggcacgat ctcggtcgac ggccccggca ccatcgagct
     2221 cgagacggcc gaggagggcg accggcccgc cgcggtcgcg ccacgcaacg gtgccggcgg
     2281 ccggcgccgt gccaccacgc cgcctccccg gcgcacgccc gtccggtagc tgccggtacg
     2341 acgccgccgg tgcgttgacc gcccggcgca acccagtcca tccaaaccca caacaggagc
     2401 agacatgccc atgaccacca cggccgggga cacccgagcc aacatcgttg ccgcctcgct
     2461 cgtgggcgcc gtcgtcctcg tgctggggtt cggatcaggc atcggcgcgg tcttcagtcg
     2521 ccacacggcg ttcaaggccg gggccgccca cggggcccat ggcacccagg cggcagccgt
     2581 cggcagcccg ggcagcgtcg cccttccggc tgccgacggt tccggtgccg ccatcctggc
     2641 ggccgagccg gtggcatcgt cccggtacgc agcgcccccg gtgatgtccg ct
//
LOCUS       DASWGG010000043         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2944858, whole genome shotgun sequence.
ACCESSION   DASWGG010000043 DASWGG010000000
VERSION     DASWGG010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2944858"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..>2147)
                     /locus_tag="VF954_00735"
     CDS             complement(13..>2147)
                     /locus_tag="VF954_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9529630.1"
                     /translation="VGVPREQLLAALAGVRGSHRVTSQDPEDQYQALEKYGRDLTEDA
                     RKGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEGLARRIVEGDVPE
                     GLKGKRLIALDLSSMVAGSKYRGEFEERLKAVLKEITDSDGEVVTFLDELHTIVGAGA
                     AEGAMDAGNMIKPMLARGELRMVGATTLDEYRKHIEKDAALERRFQPVYVGEPSVEDT
                     VGILRGLKERYEVHHGVRIQDAALVAAAMLSNRYVTGRFLPDKAIDLVDEAGSRLRIE
                     IDSMPTEIDVVERRRRQLEIERVALAKETDEASQERLAKLDEELANLTEQVDAMKAHW
                     QGEKDAITRIQSLKEELETRRGEAERYERDGDLARAAEIRYGQLPDLDRQVGEATKHL
                     AELQADQKMLKEEVDEEDVAEVVSRWTGVPVSRLMEGEVQKLVKMEDVLHERVVGQEE
                     PVRVVANAIRRSRSGLSDPNRPIGSFLFLGPTGVGKTELARALAEFMFDDERAMIRVD
                     MSEYQEKHTVARLIGAPPGYIGYEEGGQLSEAVRRRPYAVVLLDEIEKAHSDVFNVLL
                     QVLDDGRLTDGQGRTVDFSNAVLIMTSNLAGEPAEYFKPEFINRIDEIVRFRQLTRDD
                     IARIVDIQLRHLERRLAERRLSLAVTDAARAWLAVHGYDPVYGARPLKRLISRAVSDA
                     LAMALLEGRYPEGGTVVVDAGDSGIILL"
BASE COUNT          267 a          770 c          748 g          362 t
ORIGIN      
        1 ggtaccgatt gcctagagca agatgatccc gctgtcgccc gcatcgacga ccacggtgcc
       61 cccttcaggg taccgcccct cgaggagagc catggcgaga gcgtcgctga ccgcccggct
      121 gatcagccgc ttcagcggcc gggccccgta gaccgggtcg tagccgtgca cggccagcca
      181 cgcccgggcc gcgtccgtga ccgcgaggga cagccgccgc tccgcgagcc gccgttccag
      241 gtgccgcagc tggatgtcca cgatgcgggc gatgtcgtcc cgggtgagct gccggaagcg
      301 gacgatctcg tcgatccggt tgatgaactc cggcttgaag tactccgccg gctccccggc
      361 caggttggac gtcatgatga gcacggcgtt ggagaagtcg acggtccggc cctggccgtc
      421 ggtgagccgg ccgtcgtcga gcacctggag cagcacgttg aacacgtcgc tgtgcgcctt
      481 ctcgatctcg tccagcagca ccaccgcgta gggccggcgc cggacggcct ccgacagctg
      541 cccgccctcc tcgtagccga tgtagcccgg cggggcgccg atgaggcggg cgaccgtgtg
      601 cttctcctgg tactcgctca tgtcgacgcg gatcatggcg cgctcgtcgt cgaacatgaa
      661 ctccgcgagc gcgcgcgcga gctcggtctt gcccacgccc gtcgggccga ggaacaggaa
      721 gctgccgatc gggcggttgg ggtccgacag gcccgagcgc gaccggcgga tggcgttggc
      781 caccacgcgc accggttcct cctggcccac gacgcgctcg tgcaggacgt cctccatttt
      841 caccagcttc tggacctcgc cctccatgag gcgcgacacc ggcacgcccg tccagcggga
      901 cacgacctcg gcgacgtcct cctcgtcgac ctcctccttc agcatcttct ggtcggcctg
      961 gagctcggcc aggtgcttgg tggcctcccc gacctggcgg tcgaggtcgg gcagctggcc
     1021 gtagcggatc tcggcggccc gggccaggtc gccgtcgcgc tcgtagcgct cggcctcgcc
     1081 ccggcgggtc tccagctcct ccttgaggga ctggatgcgg gtgatggcgt ccttctcgcc
     1141 ctgccagtgg gccttcatgg cgtcgacctg ctcggtgagg ttggccagct cctcgtccag
     1201 cttggccagc cgctcctgcg aggcctcgtc ggtctccttg gccagggcca cccgctcgat
     1261 ctccagctgc cggcgccgcc gctcgaccac gtcgatctcc gtgggcatgg agtcgatctc
     1321 gatgcggagc cgggacccgg cctcgtcgac caggtcgatg gccttgtccg gcaggaagcg
     1381 gcctgtgacg tagcggttgg acagcatggc cgcggccacc agggcggcgt cctggatgcg
     1441 cacgccgtgg tggacctcgt agcgctcctt caggccgcgc aggatgccga cggtgtcctc
     1501 gaccgagggc tcgcccacgt acacgggctg gaagcgccgc tcgagggcgg cgtccttctc
     1561 gatgtgcttg cggtactcgt cgagggtggt ggccccgacc atgcgcagct ctccccgggc
     1621 cagcatgggc ttgatcatgt tgccggcgtc catggcgccc tcggccgccc cggcgccgac
     1681 gatggtgtgc agctcgtcga ggaaggtgac gacctcgccg tcggagtcgg tgatctcctt
     1741 gagcaccgcc ttcagccgct cctcgaactc gccccggtac ttggacccgg cgaccatgga
     1801 gctcaggtcg agggcgatca ggcgcttgcc cttgaggccc tcggggacgt cgccctcgac
     1861 gatgcggcgg gccaggccct ccacgatggc ggtcttgccc acgccgggct cgccgatgag
     1921 gacggggttg ttcttggtgc ggcgggacag gacctggatg acccggcgga tctcctcgtc
     1981 gcggccgatc acggggtcga gcttgccctt gcgggcgtcc tcggtgaggt cccggccgta
     2041 cttctccagg gcctggtact ggtcctcggg gtcctggctg gtgacccggt ggctgccccg
     2101 cacgccggcc agggcggcga gcagctgctc gcgcggcacg cccacga
//
LOCUS       DASWGG010000044         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2277565, whole genome shotgun sequence.
ACCESSION   DASWGG010000044 DASWGG010000000
VERSION     DASWGG010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2277565"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1387
                     /locus_tag="VF954_00740"
     CDS             137..1387
                     /locus_tag="VF954_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX9529631.1"
                     /translation="MPEAVTLAMSELTGAVKEGLLALAVGAGLGVLQVLMEEGVTAVA
                     GPKGRHDPGRVAKRHGTEEGSVTLGGRRVPVRRPRVRSADGRREIPVPAYETFASTEI
                     LGELALERMMAKLSTRRYRAGLEPVGQAVEATATSTSRSAVSRRFVALTERALAEMMA
                     GDLSGLDLVALMVDGVHFAEHCCIVALGIGIDGTKHPLGVVEGSTENATVVTDLLANL
                     RERGLDVTRPVLVVIDGAKALAAAVKAVFDHPVIHRCQLHKIRNVEQKLPDALASTVA
                     KKMRAAYRNPDALAAEADLEALARQLSKAHPGAAASLREGLAETLTIGRLGVPPTLAR
                     TLRSTNAIESMIEICRDHSTNVKRWRDGEMALRWCAAGMLEAAKQFRRVNGFLHLPAL
                     RAALNAHIARQTVTPIDYNEEDAA"
BASE COUNT          240 a          550 c          517 g          200 t
ORIGIN      
        1 gtggcggtcc cccgctcgag acctgcctgc tggtaggtgt cgggcgtacc gccaagtaca
       61 gccgacaaga caggggaccg ccgtgaagaa gaactaccag accacgggcg ccgccgcgcc
      121 gatcgacgcg ctggcgatgc cggaggcggt gacgctggcg atgagcgagc tcaccggggc
      181 ggtgaaggag ggtctgctcg cgctggccgt gggtgccggg ctgggggtgt tgcaggtgct
      241 catggaggag ggcgtgaccg ctgtggctgg tccgaagggt cgtcacgacc cggggcgggt
      301 cgccaagcgg cacgggacgg aggagggctc ggtcacgctc ggcgggcggc gagtgccggt
      361 gcgccgtccc cgggtccgca gcgcggatgg ccgccgggag atccccgtcc cggcctacga
      421 gacgttcgcc agcaccgaga tcctcggcga actggccctg gagcggatga tggccaagct
      481 gtccacccgc cgctaccggg ccggcctcga gccggtcggc caggccgtgg aagcgacggc
      541 gacctcgacg tcgaggtcgg cggtgtcgcg acggttcgtg gccctcacgg aacgggcgct
      601 ggccgagatg atggccggcg acctctcggg cctggatctc gtggcgctga tggtcgacgg
      661 cgtgcacttc gccgagcact gctgcatcgt ggccctcggg atcggcatcg acggcaccaa
      721 gcacccgctc ggtgtcgtcg agggctccac cgagaacgcc accgtggtca ccgacctgct
      781 ggccaacctg cgggagcggg gcctggacgt gacccgcccg gtcctcgtcg tcatcgatgg
      841 cgccaaggcc ctcgccgccg ccgtcaaagc cgtcttcgac caccccgtga tccatcgctg
      901 ccaactccac aagatcagaa acgtggagca gaagctgccc gacgccctcg cctccacggt
      961 ggccaagaag atgcgggcgg cctaccgcaa ccccgacgcc cttgctgccg aggcggacct
     1021 cgaagccctt gcccggcaac tgtccaaggc tcaccccggt gccgccgcca gcctccggga
     1081 gggcctcgct gagacgctga ccatcggccg gctcggcgtg ccgcccaccc tggcccggac
     1141 gctgcggagc acgaacgcca tcgagtcgat gatcgagatc tgccgagatc actccaccaa
     1201 tgtcaagcgc tggcgagacg gcgagatggc cctgcgctgg tgcgccgccg gcatgctcga
     1261 agcggccaag cagttccgcc gcgtcaacgg cttcctccac ctgcccgccc tccgagccgc
     1321 cctcaacgcc cacatcgccc gacagaccgt cacacccatc gactacaacg aagaagacgc
     1381 agcctgatca ccgggccgcc accgaagttc cacggaactc gggtcatcct ccgcccaacg
     1441 gcacgtccgg gcttgccgtc atcgacgacc cgctcccgcc ctcaggcatc gccgcgggcg
     1501 actggag
//
LOCUS       DASWGG010000045         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_344002, whole genome shotgun sequence.
ACCESSION   DASWGG010000045 DASWGG010000000
VERSION     DASWGG010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344002"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VF954_00745"
     CDS             <1..494
                     /locus_tag="VF954_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX9529632.1"
                     /translation="PASTRSLPSPAIEAHPDPRLQPIVDKIRSSGGKVTRARMAILEG
                     LLAGPHHATVEELEARVRMVAPDVHEATFYRTLGALEELGVVYHLHFQHGPSIWHVAG
                     EDHEHLFCQACGCTIEVASRDLEPLRAAMARRYGFVLDTRHFVHQGLCRACAARPDTN
                     RGS"
     gene            complement(528..>1862)
                     /locus_tag="VF954_00750"
     CDS             complement(528..>1862)
                     /locus_tag="VF954_00750"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX9529633.1"
                     /translation="SLGTQLAWRIVPLLAAGVLVGLAVRSPQPRRRRGAIGAAGIAGL
                     AAMWGDVESSHASAAHSLRLLRMGDQWAHFAAAGVWVGGLAALLITVGAAARDERARA
                     AARYSLAALCCVAVVAATGFQRGYDEIGSLHRLLHSGFGQYVLVKMALLGLVVGLGAL
                     NRYRSVPAVDRSPRLLQVVGRAELVLLTAVLAATGIIQGLAPPSSAAAPAVHPLVLGG
                     HDFATTVRIKLAISPGTAGFNRFTLTVADYDTGAPVPAAASLGFTLPSRPDLGSSTLA
                     LGQEETGVLGAAGANLSINGTWTVVVTVQRPTGGVQIPFSVTPRALPQRIRVQPEGSL
                     PTLYTLELPADQSVQSYLDPGHPGFDELHVTFVGAGGQELPMTALTVTATPPPGGAGP
                     LTVRHLDVRRLDDIGHFVADIPGAVKGSYQLHVSGTAAAGATIEGTFTIPVK"
BASE COUNT          261 a          645 c          729 g          227 t
ORIGIN      
        1 ccccggccag cacccgctcc cttccctccc cggcgatcga ggcccatccg gatcctcggc
       61 tgcagcccat cgtcgacaag atccggtcga gcggcgggaa ggtgacccga gcccggatgg
      121 ccatactcga ggggctgctc gccggccccc accacgccac cgtcgaggag ctggaggcgc
      181 gcgtccggat ggtggccccc gacgtgcacg aggcgacctt ctaccggacg ctcggggccc
      241 tcgaggagct cggtgtggtc taccacctcc atttccagca cggcccctcc atctggcacg
      301 tggcggggga ggaccacgag cacctgttct gccaggcctg tgggtgcacc atcgaggtcg
      361 cctcgcgcga cctggagcct ctccgcgccg ccatggcgcg gcgctacggg ttcgtgctcg
      421 acacccgcca cttcgtccac cagggcctgt gtcgcgcgtg tgcggcgcgc cccgacacca
      481 acaggggcag ctgaccgcgg cccgccatga gccgcctggc cggggcgcta cttgacaggg
      541 atggtgaagg tcccctcgat ggtggcgccc gcggcagccg tgccgctgac gtgcagctgg
      601 taggagccct tcacggcgcc ggggatgtcg gcgacgaaat ggccgatgtc gtccaggcgc
      661 cggacgtcca ggtgccgtac ggtcaaaggg ccggcgccgc ccggcggggg ggtggccgtc
      721 acggtcagcg cggtcatggg gagctcctgg ccgccggcgc cgacgaaggt gacgtggagc
      781 tcgtcgaagc cgggatggcc ggggtcgagg tagctctgca cggactgatc ggccggcagc
      841 tcgagggtgt agagcgtggg caggctgcct tcgggctgga cccggatccg ctgcgggagg
      901 gcgcgggggg tgacggagaa ggggatctgc acgccgccgg tgggccgctg gacggtgacc
      961 acgacggtcc aggtgccgtt gatggaaagg ttggcaccgg cggcgccgag cacaccggtc
     1021 tcttcctgcc cgagggccag ggtcgacgag ccgaggtccg gccgcgacgg gagcgtgaag
     1081 ccgagcgagg cggcggcggg cacgggggcg ccggtgtcgt agtcggcgac ggtgagggtg
     1141 aaccggttga acccggcggt gcccgggctg atcgcaagct tgatccgcac ggtcgtggcg
     1201 aagtcgtggc cgccgagcac gagcgggtgc acggcggggg cggcggcgct gctgggcggg
     1261 gccaggccct ggatgatccc ggttgccgcc agcacggcgg tgagcaggac cagctcggca
     1321 cggcccacga cctgcagcag ccggggcgag cggtccacgg ccggcacgga ccggtagcgg
     1381 ttgagcgcgc cgagcccgac caccagcccg agcagggcca tcttgaccag cacgtactgg
     1441 ccgaagccgc tgtggagcag gcggtgcagg ctgcctatct cgtcgtagcc gcgctggaag
     1501 ccggtggccg cgacgacggc cacgcagcac agcgcggcga gcgagtagcg ggcggcggcc
     1561 cgggcgcgct cgtcgcgggc ggccgcgccg acggtgatca gcagtgcggc caggcctcca
     1621 acccagacgc cggccgccgc gaagtgagcc cactggtcgc ccatgcgcag cagccgcagc
     1681 gagtgggcgg ccgacgcatg gctcgattcc acatctcccc acatggcggc cagcccggcg
     1741 atgccggctg ctccgatcgc gccgcgccgc ctgcgcggct ggggggatcg cacggcgagt
     1801 ccgacgagga ccccggcggc gagcaggggg acgatccgcc acgccagctg ggtgccgagc
     1861 ga
//
LOCUS       DASWGG010000046         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1657625, whole genome shotgun sequence.
ACCESSION   DASWGG010000046 DASWGG010000000
VERSION     DASWGG010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657625"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /gene="pheS"
                     /locus_tag="VF954_00755"
     CDS             complement(<1..1011)
                     /gene="pheS"
                     /locus_tag="VF954_00755"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX9529634.1"
                     /translation="MSVALGPQLEALEADGRARLAGAADLDELRGAEADVLGRGSALA
                     RLNQGLRELAPDERKAAGAAIQAARARLQDLAEGRRQELSAAARRARVEADRLDLTEA
                     PDLVPGRGHLHLVSQTQAELEDVFLGMGYVVAEGPEAETDWHNFEALNLPRDHPARGM
                     WDTLYLRLNEPESTLLRTHTSPVQVRLMESQDPPIYAVCPGRVYRRDTPDARHTPIFH
                     QIEGLVVDRGITFGDLAGTIETFTTAYFGPAIHSRLRPSYFPFTEPSAEYEVTCPICE
                     GRGCRTCSGTGWIELGGCGLVDPNVFAAVGIDAEEWSGFAFGFGIDRCAQVRHQLPDM
                     RVL"
     gene            complement(1008..>1930)
                     /locus_tag="VF954_00760"
     CDS             complement(1008..>1930)
                     /locus_tag="VF954_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9529635.1"
                     /translation="LLDPRSRNGHMVWNGSWGAASSLRSVRSIPEQEITIRHANGEDT
                     RVFATGAYQRLAGGALSGAIVVLRSASRRGYQAAAGIEIVSTVSHELRSPLTSVKGYT
                     SLLLNRWDRLRDDQKRMMLEQVNHDADRVTRLITELLDISRLETGRLVLRRQLVDLPE
                     LTAGVVDKLRMEHPDLGASLEFPAVFPKVYADPDKVVQVLTNLVENACKYASPIGLLV
                     KGASNQTDVSVAVTDRGEGIPRDDLPKVFTKFFRRAEGKPTGSGLGLWISRGLVEAHG
                     GRLTATSAPGEGTTFRFTLPLVDFEELHSS"
BASE COUNT          258 a          682 c          713 g          277 t
ORIGIN      
        1 caggacccgc atgtcgggga gctggtgacg gacctgggcg cagcggtcga tgccgaagcc
       61 gaaggcgaag cccgaccatt cctcggcgtc gatgcccacg gcggcgaaga cgttggggtc
      121 gaccaggccg cagccgccca gctcgatcca cccagtgccg gagcaggtcc tgcagccccg
      181 gccctcgcag atcgggcagg tgacctcgta ctcggcggaa ggctccgtga aggggaagta
      241 cgacgggcgc aggcgcgagt ggatggccgg gccgaagtac gccgtggtga aggtctcgat
      301 cgtgccggcc aggtccccga aggtgatgcc ccgatcgacg accagcccct cgatctggtg
      361 gaagatcggg gtgtggcggg cgtcgggcgt gtcccggcgg tacacccggc ccgggcacac
      421 ggcgtagatc ggcgggtcct gggattccat caggcgcacc tggacgggtg aggtgtgggt
      481 ccgcagcagg gtcgactcgg gctcgttcag gcgcaggtac agggtgtccc acatgccgcg
      541 cgccggatgg tcccgcggga ggttgagggc ctcgaagttg tgccagtcgg tctcggcctc
      601 gggtccctcg gccaccacgt agcccatgcc gaggaacacg tcctccagct cggcctgcgt
      661 ctggctcacc aggtgcaggt gcccgcgacc gggcaccagg tcgggcgctt cggtcagatc
      721 gagccggtcg gcctccaccc gggcccggcg ggcggcggcg gagagctcct ggcggcgccc
      781 ttcggccagg tcctggagcc gggcccgggc ggcctggatg gccgcgcccg ccgccttgcg
      841 ctcgtcgggc gccagctccc gaaggccctg gttgagccgg gccagcgccg agccccggcc
      901 caggacgtcg gcctcggccc cccgcagctc gtccaggtcg gccgcgccgg cgaggcgggc
      961 gcggccgtcc gcctccagcg cctcgagctg tgggccgagc gccacgctca cgaggagtgc
     1021 agctcctcga agtccaccag cggcagcgtg aagcggaacg tcgtgccctc ccctggcgcg
     1081 gaggtcgccg tcaggcgccc gccgtgggcc tcgaccaggc cccggctgat ccacaggccc
     1141 agccccgagc cggtcggctt gccctcggcc cgccggaaga acttggtgaa caccttgggc
     1201 aggtcatcgc gagggatgcc ctcgccccgg tcggtgacgg ccaccgagac gtcggtctgg
     1261 ttggacgcgc ccttgaccag caggccgatg ggcgaggcgt acttgcatgc gttctcgacc
     1321 aggttggtca gcacctgcac caccttgtcg ggatcggcgt acaccttggg gaacaccgcc
     1381 gggaactcca ggctggcgcc caggtcggga tgctccatgc gcagcttgtc gaccacgccc
     1441 gcggtcagct cggggaggtc cacgagctgg cgccgcagca ccagccggcc cgtctccagc
     1501 cggctgatgt cgagcagctc ggtgatgagg cgggtcaccc tgtctgcgtc gtggttgacc
     1561 tgctcgagca tcatgcgctt ctggtcgtcg cgcagccggt cccagcggtt cagcagcagg
     1621 ctcgtgtagc ccttgaccga cgtgagcggc gaccgcagct cgtggctcac ggtcgacacg
     1681 atctcgatgc cggccgcggc ctggtagccg cggcggctgg cgctgcgcag caccactatg
     1741 gccccggaga gcgccccgcc ggccaaccgc tggtatgcgc ccgtggcgaa cacccgggtg
     1801 tcctcaccgt tggcgtggcg gatggtgatc tcctgctccg ggatgctgcg gaccgagcgc
     1861 agggagctgg ccgcgcccca gctgccgttc cacaccatgt ggccgttgcg gctgcgcggg
     1921 tcgaggagcg
//
LOCUS       DASWGG010000047         5655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_22362, whole genome shotgun sequence.
ACCESSION   DASWGG010000047 DASWGG010000000
VERSION     DASWGG010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22362"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VF954_00765"
     CDS             complement(<1..253)
                     /locus_tag="VF954_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529636.1"
                     /translation="MTAITLPSSAGAWSTSRTIGWPAEVARRPEVVGGSAGGRGVASR
                     FGGGGTRAQPPSVYRRRRLGAVLVVATVALGARALALGLA"
     gene            554..1189
                     /gene="lexA"
                     /locus_tag="VF954_00770"
     CDS             554..1189
                     /gene="lexA"
                     /locus_tag="VF954_00770"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX9529637.1"
                     /translation="MTKTTVLTGKRREILDFISEELRTRGYPPSVREIGEAVGLHSSS
                     TVHNHLATLQRQGYLRRDPTKPRAIEVRYDPSSGAAVESRPVRHVPVVGDVAAGANVL
                     AQENVEELMPLPEDFTGTGQLFMLRVRGDSMIDAGILDGDYVVVRQQPEANARDIVVA
                     GVGDGEATVKTFTRKGNKIVLVPANPRLSPISYDPAEVTIFGKVVTVLRRL"
     gene            1279..2721
                     /locus_tag="VF954_00775"
     CDS             1279..2721
                     /locus_tag="VF954_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9529638.1"
                     /translation="MTRCWARAAVTVVFALGLLARTALPARAFTANDPFFTNGAQWSL
                     QAINAPRGWDVATGKGTTIAVVDTGVDLPHEDLAAKIDTADAWNFVANDGNAQDDNGH
                     GTHVSGIAAAITGNGKGIAGVAPDARIMPVKVLDSRGSGTEGNVELGIRWAADHHATV
                     INLSLGDPTSQSFAGVALSGSTALGDAVQYAWSHGSIVVVAAGNSYVTSSSFSTEPAL
                     VVAATGRHNETPSYSSGVGSAQWGISAPGGSGEMAPLTANADDVISTYWDGSAQEDNA
                     YAYDAGTSMAAPHVAGAAAVLRSLGLTPQQTVDRLLKTATPLGSSSQNGAGLLNLGAA
                     VAGLGPAPVASPTTAPARSSGTTARPPSATPARSPTAPASGGAGAGARNPGSASPSGG
                     VSPAPGAAVTAGAAGSPAPEVAGADLGGAPARSPSLAGAHDRSDRPWAWAALAGLALG
                     AVAGGTGIVTLRRGPRRAPGGMGSPPVTPA"
     gene            complement(2708..3787)
                     /gene="dapE"
                     /locus_tag="VF954_00780"
     CDS             complement(2708..3787)
                     /gene="dapE"
                     /locus_tag="VF954_00780"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX9529639.1"
                     /translation="MTDLLAAAAELVDIPSLSHHEGALADRLESDLGAAPWLAVERVG
                     DNVVARTDLGRDNRLILAGHTDTVPPNGNGRARLDGDVLWGLGSADMKAGLAVFRELA
                     LTVSEPEMDVTYVFYACEEVDQAHNGLVQLLRDRPDLLDGDAAVLGEPTGGRVEAGCQ
                     GTMRAKLVIRGERAHTARPWVGANAIHRLGPFLHRLAGYEGRRPVLDGCEYREALQAV
                     GVEGGVANNVVPDEVTLTLNHRYAPDRRTGEAFLHLVEIFEPLLDRGRGDGLELVADS
                     IPAPPGLTHPLLARLVQLTGRPPAAKLGWTDVSFFASSGIPATNFGPGDPLLAHTAGE
                     RVERHDIEQAFTLLQGYLRDDPKRG"
     gene            complement(3784..4611)
                     /locus_tag="VF954_00785"
     CDS             complement(3784..4611)
                     /locus_tag="VF954_00785"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX9529640.1"
                     /translation="MADLQAHIEELWARQDELGPGDGDASDLVHRAVGLLDSGEARVA
                     EVDPASGRVIVHEWLKQAILLLFRVSAMETIEAGPFVYADKLPLKRDFARAGVRAVPG
                     ASARWGSYLERGVILMPSYVNIGARVGANTMVDTWATVGSCAQVGANVHLSGGVGIGG
                     VLEPPQAAPVVIEDDCMIGSRSMVTEGSRVGRGSVLGAGTILAGSIPVIDAETGEEIS
                     RGEVPSSCVAVTANRRRQYAGGEFFLPCVLVVKRLEEGRRHDKAQLNAILRHEGLTT"
     gene            4724..>5655
                     /locus_tag="VF954_00790"
     CDS             4724..>5655
                     /locus_tag="VF954_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529641.1"
                     /translation="MADEESEQTTPGDGEFDAWIAELRETEAEAAPSLDLGERMARLE
                     QLLERSLGANAALAERLGALSEDVRGSRGYESPAYSGPAEAGSTIPAVDLAPLLAALS
                     RSADDLDAGLATVRAAVSTSQKGVAARLDDLWLTVTRRDELLQQHLEQRLDDLLSLAT
                     TPTPAPGPAALPRAPDPDDEPVDAEPVDAEPIDAEPVDARSVGTEPGALARPGDIEEL
                     QRRLVDEVRVELEVRAQDTRDAFSVLARHLTDIEDRLAGLAGAERPGEDSGAEQLEQL
                     ASRIGAHLREQDGSGDREALTDAMARIDAALAPDG"
BASE COUNT          823 a         2129 c         1980 g          723 t
ORIGIN      
        1 cggccaaccc cagggcgagg gcccgagccc cgagcgcgac cgtggccaca accagcacgg
       61 cgcccagccg gcggcgccgg tagaccgagg ggggttgggc ccgggttccc ccgccgccga
      121 accgcgacgc gacgccgcgc ccgcccgctg agccgcccac cacctcgggc cggcgcgcca
      181 cctcggcggg ccagccgatg gttcgcgacg ttgaccaggc accggctgaa gaagggagcg
      241 tgatggcggt catgggagct ctccggtcga aggcggcttg gacggcttgg acgcccgtgc
      301 gttcgcccgc tccgcccggg ctgcccccgg cggacgaacg cacgttcgtg agacccaccc
      361 aaccacgaac ccgcgttcga tgtcaaggcc catacgaaca aatgtttgtc ccagagcgcg
      421 gactccgcta tgatccgaac tggcgttcgc tcgccggtcc agggcaatgc agtcggcaat
      481 gcagtctcag ggcaatgcag tctcagggca atgcagtctc cggaccattg ctggcaggac
      541 acgagggagg acgatgacaa agaccacggt gctgaccggc aagcgtcggg agatcctcga
      601 cttcatatcc gaggagctgc ggacccgcgg ctacccgccc tcggtgcgcg agatcgggga
      661 ggcggtcggc ctgcactcgt cctcgacggt ccacaaccac ctcgccaccc tccagcggca
      721 gggctatctg cggcgggacc ccaccaagcc ccgggccatc gaggtccgct acgacccgtc
      781 gtcaggggct gctgtcgaga gccggccggt ccgtcacgtc ccggtggtcg gcgacgtggc
      841 cgccggcgcc aacgtgctgg cccaggagaa cgtcgaggag ctcatgccgc tccccgagga
      901 cttcacgggc acggggcagc tcttcatgct gcgggtccgg ggcgactcga tgatcgacgc
      961 ggggatcctg gacggagact acgtcgtggt ccgccagcag cccgaggcca acgcccgcga
     1021 catcgtcgtg gccggcgtcg gcgacgggga agccaccgtc aagaccttca cccgcaaggg
     1081 caacaagatc gttctcgtgc ccgccaaccc ccggctatcg ccgatctcgt acgaccccgc
     1141 cgaggtgacg atcttcggca aggtggtgac ggtcctgcgc cgtctctgaa tcggcgccga
     1201 ggtgcgccgc ggagcgagcc aaggagtcct cgaaggatcc cgggccccga ggggaccgct
     1261 gggcgcgaca atggtcgcat gacgcgatgc tgggcgcggg ctgcggtgac ggtcgtcttc
     1321 gcgctcgggc tgctggcccg gacggcgctg ccggcgcgag ccttcacggc caacgacccc
     1381 ttcttcacca acggcgccca gtggagcctc caggccatca atgcccccag aggatgggac
     1441 gtggcgaccg ggaagggaac cacgatcgcg gtcgtggaca ccggcgtcga cctgccgcac
     1501 gaggacctgg cggccaagat cgacaccgcc gacgcctgga acttcgtggc caacgacggc
     1561 aacgcccagg acgacaacgg gcacggcacc cacgtgtcgg ggatcgccgc ggccatcacc
     1621 ggcaacggga agggcatcgc cggggtggca cccgatgccc gcatcatgcc ggtcaaggtg
     1681 ctcgacagca ggggctccgg gacggagggc aacgtcgagc tcgggatccg ctgggccgcc
     1741 gatcaccacg ccacggtcat caacctctcg ctgggcgacc ccaccagcca gtcgttcgca
     1801 ggggtcgccc tctccgggag cacggccctc ggcgacgccg tgcagtacgc gtggtcgcac
     1861 gggagcatcg tcgtcgtcgc cgccggcaac agctacgtca cgtcgtcgtc gttcagcacc
     1921 gagcccgcct tggtcgtggc cgcgaccggc cgccacaacg agaccccgag ctactcgagc
     1981 ggcgtgggct cggcccagtg gggcatctcc gcgcccggag ggtcagggga gatggctccc
     2041 ctcaccgcca acgccgacga cgtcatctcg acctattggg atggctcggc ccaggaggac
     2101 aacgcctacg cctacgatgc gggcacgtcg atggccgcgc cccacgtggc gggcgccgcc
     2161 gcggtgctgc gctcgctcgg cctgacgccg cagcagacgg tggaccggct gctcaagacg
     2221 gcgaccccgc tcggctcgtc ctcgcaaaat ggggccggcc tgctcaacct cggcgccgcc
     2281 gtggccggcc tcggcccggc gccggtcgcc tcccccacca cggccccggc ccgctctagc
     2341 ggaacaacgg cgcgtccgcc gagcgccacc ccggcccgga gcccgaccgc gcccgcgagc
     2401 ggtggagccg gtgcgggggc ccgcaacccg ggctcggcca gcccttcggg tggggtgtcg
     2461 cctgcccctg gcgccgccgt gactgcgggc gcagcgggct ctccggcacc ggaggtggcc
     2521 ggcgcggatc tgggcggcgc ccccgcccgc tcgccgtcgc tcgccggcgc tcacgaccgg
     2581 tccgaccgcc cgtgggcctg ggcggcgctc gcaggtctgg cgctcggggc cgtcgccgga
     2641 gggacgggca tcgtcaccct tcgccgtggt ccccggcggg cccccggcgg gatgggatcg
     2701 ccgcccgtca cccccgctta gggtcatccc gcaggtaccc ctggagcagg gtgaacgcct
     2761 gctcgatgtc gtgtcgctcg acccgctctc cggccgtgtg ggcgaggagc gggtcgcccg
     2821 ggccgaagtt cgtggccggg atgccggacg aggcgaagaa ggacacgtcc gtccacccca
     2881 gcttggccgc cggcggacga ccggtcagtt gcaccagccg ggcgagcagg ggatgcgtga
     2941 ggccgggcgg tgccgggatg gagtcggcga ccagctccag gccgtcgccc cgtccccggt
     3001 cgagcagggg ctcgaagatc tccaccaggt ggaggaaggc ctccccggtg cgccgatcgg
     3061 gggcgtagcg atgattcagc gtcagcgtga cctcgtcggg caccacgttg ttggccacgc
     3121 ccccttcgac tccgacggcc tggagggcct cccggtactc gcagccgtcc agtaccgggc
     3181 gccggccttc gtagccggcc agcctgtgca ggaacggccc gaggcggtgg atggcgttgg
     3241 cgccgaccca gggtcgggcc gtgtgcgccc gctcgccgcg tatgaccagc ttggcgcgca
     3301 tggtcccctg gcaccccgcc tccacccggc ccccggtggg ctcccccagg acggccgcgt
     3361 ctccgtcgag gaggtcgggc cggtccctca acagctggac gagcccgttg tgggcctggt
     3421 ccacctcctc gcacgcgtag aagacgtacg tcacgtccat ctccggctcg gacaccgtga
     3481 gcgccagctc ccggaacacc gccaggcccg ccttcatgtc cgccgacccc aggccccaga
     3541 gaacgtcgcc gtccagccgg gcccgaccgt tgccgttggg cggcacggtg tcggtgtggc
     3601 cggccaggat cagccggttg tcccggccga ggtcggtgcg ggccactacg ttgtcgccga
     3661 cccgctcgac cgccagccat ggggccgccc ccaggtccga ctcgagccgg tcggcgaggg
     3721 ccccctcgtg atggctgagc gacgggatgt ccaccagctc ggcggcggcg gccaacaggt
     3781 cggtcacgtg gtgagcccct cgtggcgaag gatggcgttg agctgggcct tgtcgtggcg
     3841 ccgaccttcc tccaggcgct tgaccacgag gacgcacggc aggaagaact ctcctcccgc
     3901 gtactgccgc cgccggttgg ccgtgaccgc gacacacgag ctcgggacct cgccccggct
     3961 gatctcctcg cccgtctcgg cgtcgatcac cgggatcgat ccggccagga tggtgcccgc
     4021 gcccagcacc gatccccggc ccacgcggga gccctcggtg accatcgaac ggctaccgat
     4081 catgcagtcg tcctcgatca cgaccggcgc ggcctgcgga ggctcgagca ccccgccgat
     4141 cccgaccccg cccgagagat gcacgttggc gccgacctgg gcgcacgagc cgaccgtcgc
     4201 ccacgtgtcc accatggtgt tggcaccgac ccgagctccg atgttgacgt agctcggcat
     4261 gaggatgacg ccgcgctcca ggtacgagcc ccaccgggcg gacgcgcccg ggacggcccg
     4321 gaccccggcc cgggcgaagt cgcgcttcag cggcagcttg tcggcgtaca cgaatgggcc
     4381 ggcctcgatg gtctccatgg ccgacacccg gaacagcagg aggatggcct gcttcaacca
     4441 ctcgtgcacg atcacccggc ccgatgccgg gtccacctcg gccacccggg cctcgccgct
     4501 gtccagcagc ccgacggctc ggtgcaccag gtcggaggca tcaccatcgc ccgggccgag
     4561 ctcgtcctgg cgcgcccaga gctcctcgat gtgggcctgc aggtcagcca tgcggcgaag
     4621 gctaccgagc gaggccagcg ggcaccgtaa ccgagggatg gaccggaacc gcccgcgtcc
     4681 cgcgccatac tcgtagggtc ggcgttcgcc gaggaggaag ttgatggcgg acgaagagtc
     4741 cgagcaaacc accccaggcg acggcgagtt cgacgcctgg atcgccgagc tgcgcgagac
     4801 cgaggccgag gccgccccct ccctggacct cggtgagcgc atggcccgcc tcgagcagct
     4861 gctcgagcga tcgctgggcg ccaacgcggc cttggccgag cggctgggcg cgctcagcga
     4921 agacgtacgg ggctcccgag gctacgagtc gcccgcatac tcggggccgg ctgaggccgg
     4981 ctccacgatc ccggcggtag acctggcccc gctcctggcg gccctgagcc gttcggcgga
     5041 cgatctcgac gccgggctgg ccacggtgcg agccgccgtc tccacgtccc agaagggtgt
     5101 ggccgcccgc ctggacgacc tgtggctgac tgtcacgcgc cgcgacgagc tgctgcaaca
     5161 gcacctcgag cagcgcctcg acgacctcct gtcgctggcc accacgccca ccccagcacc
     5221 cggccctgcc gccctgcccc gggcacccga tccggacgac gagcccgtcg atgccgagcc
     5281 cgtcgatgcc gagcccatcg atgccgagcc cgtcgatgcc cggtccgtcg gtaccgagcc
     5341 cggggcgctg gcacgccccg gcgatatcga ggagctccag cgccgcctcg tcgacgaggt
     5401 ccgggtcgag ctggaggtgc gggcccagga cacccgcgat gcgttctcgg tcctggcccg
     5461 ccatctgacc gacatcgagg accgcctcgc cgggctggcc ggcgccgagc ggcccggcga
     5521 agactcgggg gccgagcaac tcgagcaact ggcatcccgc atcggagccc atctccggga
     5581 gcaggacgga tcgggcgacc gcgaggccct gaccgatgcc atggcgcgga tcgacgccgc
     5641 gctcgcgccc gacgg
//
LOCUS       DASWGG010000048         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_23180, whole genome shotgun sequence.
ACCESSION   DASWGG010000048 DASWGG010000000
VERSION     DASWGG010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23180"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VF954_00795"
     CDS             <1..347
                     /locus_tag="VF954_00795"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HEX9529642.1"
                     /translation="PVALAVTILTSDPSAPAHVLGNRVRVALEAGCGGLVCAASDIRE
                     AKQLAPRLVAVVPGIRPAGAAPDDQARIATPEAALAAGADLLVIGRSVTAADSRVGAA
                     AAIGQAIMGSSR"
     gene            359..679
                     /gene="mihF"
                     /locus_tag="VF954_00800"
     CDS             359..679
                     /gene="mihF"
                     /locus_tag="VF954_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX9529643.1"
                     /translation="MPLPPSLSPEQRQAALQKAAVARRQRAEIKEKLKMGSLSLSELL
                     VAGDTDQVVGKMKVLAVLESLPGLGKVKARRLLEEVGISDTRRLQGLGANQRDALLRE
                     TKRP"
     gene            685..1251
                     /locus_tag="VF954_00805"
     CDS             685..1251
                     /locus_tag="VF954_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX9529644.1"
                     /translation="MLSAPGGAGKSSIISRLLAADPRLHFSRSWTTRPRRPGEPEDWY
                     HFVDRSIFEARRDAGGFVEWAEVAGNLYGTPTLEVPPGRDLLLEIDVQGARQILDQHP
                     EAVVILVVPPSRDIQRRRLEARGDSPEVIARRLAMSEKEEADGRLLAGHLVVNDDLHR
                     AVDEVAGILQRHRRTHDDPRDDRSPEGA"
     gene            1251..1520
                     /gene="rpoZ"
                     /locus_tag="VF954_00810"
     CDS             1251..1520
                     /gene="rpoZ"
                     /locus_tag="VF954_00810"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX9529645.1"
                     /translation="MAQSRPTMMDPPIERLLDKVDSKFTLVTLAAKRGRQINSYFNQL
                     GEGLGTIVPPQVASVSRKPLSIALEEIAAAKIVYVRPDDVEEAPK"
     gene            1526..>2032
                     /locus_tag="VF954_00815"
     CDS             1526..>2032
                     /locus_tag="VF954_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX9529646.1"
                     /translation="MAAPVDGPSLDGRRIVLGVCGGIAAYKAAELCRRLVDAGAHVVP
                     VLTASAGRFVGPVTFSALGSEPVRTSLWDDTDPIPHTRLGQTADLVLVAPATARLIGS
                     YAAGISSDLLTATLLATRAPVLLCPAMHTEMWEHPAVRDNLATLRRRGVHIVEPGAGR
                     LAGGDTGAG"
BASE COUNT          280 a          756 c          721 g          275 t
ORIGIN      
        1 tgcccgtggc cctggcggtg accatcctca cgagcgaccc ctccgccccg gcccacgtgc
       61 tcggcaaccg cgtccgtgtg gcgctggaag ccggatgcgg ggggttggtg tgcgccgcgt
      121 ccgacatccg cgaggccaaa cagctcgcgc cccggctggt cgcggtcgtc cccggcatcc
      181 gccctgccgg cgccgcaccg gacgatcagg cccggatcgc gacgcccgag gctgccctgg
      241 ccgcgggcgc cgacctgctc gtgatcggcc ggtccgtcac ggccgccgac agccgggtcg
      301 gggccgcggc cgccatcggc caagccatca tgggctcgtc gcgctagggt cgcgacccat
      361 gccgttgcct ccctcgcttt cgcctgagca gcgtcaggcc gcgctccaga aggcggccgt
      421 cgcccggcgc caacgggccg agatcaaaga gaagctcaag atgggctcgc tcagcctgag
      481 cgagcttctg gtggccggcg acaccgatca ggtcgtcggc aagatgaagg tgctcgccgt
      541 gctcgaaagc ttgccggggc tgggaaaggt caaggcccgg cgcctgctcg aggaggtggg
      601 catcagcgac acccgccggt tgcagggctt gggcgccaac cagcgcgatg ccctgctccg
      661 ggagaccaag cggccctgat cgccatcctc tcggcacccg gtggtgccgg caagagctcg
      721 atcatcagcc gcctcctggc cgccgacccg cgcctgcact tcagccgttc ctggacgacg
      781 cggccccgcc ggccgggtga gcccgaggac tggtaccact tcgtcgaccg gagcatcttc
      841 gaggcccggc gcgacgccgg cgggttcgtg gagtgggccg aggtcgccgg caacctgtat
      901 ggcacgccca cgctggaggt gccccccggc cgggacctgc tgctcgagat cgacgtccag
      961 ggggccaggc agatcctgga ccagcatccc gaggcggtgg tgatcctcgt ggtcccgccg
     1021 tcgcgtgaca tccagcgccg ccggctcgag gcccggggcg actcgccgga ggtcatcgcc
     1081 cggcgcctgg ccatgtcgga gaaggaggag gccgacggcc ggctgctggc cggccacctg
     1141 gtcgtcaacg acgaccttca ccgggcggtc gacgaggtcg ccggtatact ccagcgtcac
     1201 cgccggacgc atgacgaccc gcgtgacgac cgcagccctg aaggagcctg atggcgcaaa
     1261 gccgcccgac catgatggac ccgccgatcg agcgcctgct cgacaaggtg gactcgaagt
     1321 tcacgctcgt caccctcgcg gccaagcggg gccggcagat caactcctac ttcaaccagc
     1381 tgggcgaggg gctcggcacc atcgtgccgc cccaggtggc gtcggtgtcc cgcaagccgc
     1441 tgtccatcgc gctggaggag atcgccgcgg ccaagatcgt gtacgtccga ccggacgacg
     1501 tcgaggaagc tcccaaatag cgacggtggc tgccccggtg gacgggccgt ccctggacgg
     1561 gcgcaggatc gtgctgggag tctgcggggg gatcgcggcc tacaaggccg cggagctgtg
     1621 ccgccgcctg gtcgacgccg gcgcccacgt cgtgcccgtc ctcaccgcct cggcggggcg
     1681 cttcgtcggc ccggtcacct tctcggcgct gggctccgag ccggtgcgca cctcgctgtg
     1741 ggacgacacc gatcccatcc ctcacacccg cctcgggcag accgcggacc tggtgctggt
     1801 ggcgccggcc acggcccggc tcatcgggtc ctacgcggcg ggcatctcca gcgacctgtt
     1861 gacggcgacc ctgctcgcca cccgggcgcc ggtgctgctg tgcccggcca tgcacaccga
     1921 gatgtgggag caccccgccg tccgggacaa cctggccacc ctccgacgcc ggggcgtgca
     1981 catcgtggag cctggtgccg gccgcctggc cggcggggac accggagccg gg
//
LOCUS       DASWGG010000049         4123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_23637, whole genome shotgun sequence.
ACCESSION   DASWGG010000049 DASWGG010000000
VERSION     DASWGG010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23637"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VF954_00820"
     CDS             <1..934
                     /locus_tag="VF954_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529647.1"
                     /translation="PAGRNAEVAVVCGPGGTGASTVAIALAQGLADATGPSGVLLADF
                     ALRGELAMLHDARDVVPSVQELVEAHRSGRPTGEDVRAMTFGISERGYHLLLGLRRSR
                     SWSSLRPRALEAAVKSLRQTFRVVVCDIDPLFEGEAETGSLDVEERHAMSRVATAAAG
                     AVVVVGACGMKGLHSLVSVVADILDFGVPAGRIVPVFNRAPRSPRARSELAQGFAALL
                     PDAAATALPAPIFLPERAVEEALRDGVRLPVALTDPLTGAYRAVIQRSGTSSLVVTGA
                     EPQLVRPGSLGGWAGPDDERSEVGDTPPDAAAVG"
     gene            1000..2664
                     /locus_tag="VF954_00825"
     CDS             1000..2664
                     /locus_tag="VF954_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529648.1"
                     /translation="MVPLVASRRATPAVQAGIAAGLAALLTAGMLWRGPAGADLAAHV
                     YQRSLYLQHGLSLWNNFWYAGRYSFITYSVLYYPLAAGLHINPLTVLTVGASAGAFTL
                     VLERQFGRSARWSAWSFVLMWPVGVVVSGVFPFALGMALTLCALVALQRRRVPWFAAL
                     AGASLLASPLAFLILMLPLASVAIAQRTNWRRWTAPVLVIVTIGLYELVVWRLFPDGG
                     RYPFWGDDFTGTLVFCLVGIVLSWRVDRASSLRWLFVVYGAVAIGAFLVSSDLGSNIA
                     RLRLAAIPLGLLVLPMRRWKPWPICVAALGLAVYWNVAPIATTYANTASDPSGSATFW
                     QPAVGWFHEHLSPDYRVEAVDTVSHWPAVFLPRAGIPLARGWFRQDDFPQNLVLYQSP
                     LTPDAYLSWLRTMGVGYVVASDAPPDFSAVNEAALLAGGQSGLPRVFSQGHVSIYAVP
                     NPSPMVTGLFPARVLTITESQVVLAVSQPASYRVAVRYSPYWRSPNACVEPAPDGMTN
                     IVAESPGIVPLTFRINAGAAMKALTGQPPHWCGGEDTSVLSAGRPG"
     gene            complement(2824..3732)
                     /locus_tag="VF954_00830"
     CDS             complement(2824..3732)
                     /locus_tag="VF954_00830"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEX9529649.1"
                     /translation="MFPLTGLLVNNSPNAARPALSVKIDNVAQARPQSGLNGADVVTV
                     ELVEGGLTRLFVTFQSQDVATMGPIRSARPVDADLLHELNGGIFAYSGAANGEIAPVI
                     DHGGAVRLSHDAGDPGFHVDRSRRAPDQVYGSTADFYTAGLKRGHPAAPGQLFTYAAQ
                     PPATGGAPVPGVKMAYSNSASDAWRWDAPSGHWVRAQDGSPDVTTDNGQVFTTNVVVL
                     QVAIHGTGIFDQAHNEDPLPVVTGTGTGWVLRDGQMFQIGWARPTFNDALQLHLPSGA
                     VMPLHPGSTWIELLPTPYVPVPGPGT"
BASE COUNT          517 a         1481 c         1468 g          657 t
ORIGIN      
        1 gccggcgggc cgcaacgccg aggtggccgt ggtctgcggg ccaggcggca ccggtgcttc
       61 gaccgtcgcc atcgcgttgg cgcaaggtct ggcggacgcc accgggccga gcggggtgct
      121 gctcgccgac ttcgcgctgc gcggggagct cgccatgctc cacgatgccc gggacgtggt
      181 cccttcggtg caggagctcg tcgaggcgca ccggtcgggc cggccgaccg gcgaagacgt
      241 gagagccatg accttcggca tctcggagcg cggctatcac ctcttgctcg ggctccgccg
      301 atcgaggtca tggtcgtcgt tgcgcccccg ggccctggag gccgccgtga agagcctgcg
      361 tcagaccttc cgggtagtgg tgtgcgacat cgaccccctc ttcgagggcg aggccgagac
      421 aggttcgctc gacgtcgagg agcgccacgc catgagcagg gtcgcgaccg ccgccgcggg
      481 cgccgtcgtg gtggtcggag cctgcgggat gaaaggcctg cactccctcg tgagcgtcgt
      541 cgccgacatc ctcgacttcg gcgtgcccgc cggtcggatc gtgccggtgt tcaatcgggc
      601 tccacgcagc ccgcgcgcgc gctcggagct cgcccaaggg ttcgccgctc tgcttcctga
      661 cgccgcggcc acggcgttgc cggcgccgat cttccttccg gagcgggccg tcgaggaagc
      721 cctgcgggac ggcgtccgct tgcccgtcgc gctgaccgat ccgctgaccg gcgcctaccg
      781 cgccgtgatc cagcggtccg gcacctcctc cctcgtggtc acgggcgccg agccccagct
      841 ggtccggccg ggctccctcg gtgggtgggc cggaccggac gacgagagga gcgaggtggg
      901 cgacaccccg ccggacgccg ccgcggtggg ttgacgccgc gcctgcgccg ggggaaaact
      961 cggctcggac cggtccggtt gagtagcgtc tcgtccacca tggtccccct ggtggcgtcg
     1021 cggcgtgcca cgccggccgt gcaggcgggc atcgcggccg gtctggccgc cctgctcacg
     1081 gccggcatgc tgtggcgcgg gcccgccggc gccgacctcg ccgcccacgt ctaccaacgc
     1141 agcctgtacc tccagcacgg cctgtcgctc tggaacaact tctggtacgc cggccgctac
     1201 agcttcatca cctacagcgt cctgtactac cccctggccg ccgggttgca catcaacccg
     1261 ttgacggtgc tgaccgtggg ggcatccgcc ggggccttca ccctcgttct ggagcgccag
     1321 ttcggccggt cggctcgctg gtcggcctgg agcttcgttc tcatgtggcc cgtcggcgtg
     1381 gtcgtctccg gcgtgttccc tttcgccctc gggatggcac tgaccctgtg cgccctcgtc
     1441 gccctgcaac ggcggcgggt cccctggttc gcggctctcg ctggggccag cctgctcgcc
     1501 agccccctcg cgttcctcat cctgatgctg ccgctggcca gcgtggccat cgcccaacgg
     1561 accaactgga ggcgctggac cgcgcccgtc ctggtgatcg tcaccatcgg actgtacgag
     1621 ctggtggtgt ggaggctctt ccccgacggc ggccgctatc ccttctgggg cgacgacttc
     1681 acgggcacgc tcgtgttctg cctcgtcggc atcgtgttga gctggagggt cgatcgagcg
     1741 tcgagcctgc ggtggctgtt cgtggtgtac ggcgcagtcg ccatcggggc cttcctcgtg
     1801 tcgtccgatc tcgggtccaa catcgcgcgc ctgcggttgg cggccatccc gctcggcctc
     1861 ctcgtcctgc ccatgcggcg gtggaagccc tggccgatct gcgtcgcggc cctggggctg
     1921 gcggtgtact ggaacgtggc ccccatagcc acgacctacg ccaacacggc cagcgacccc
     1981 agtggcagcg ctactttctg gcaaccggcc gtgggctggt tccacgagca tctcagcccc
     2041 gactaccggg tggaggcggt cgacaccgtg agccactggc cggccgtgtt ccttccccgg
     2101 gccgggatcc cgctggctcg gggctggttc cgccaggacg acttcccgca gaacctggtg
     2161 ctctaccaga gccccttgac cccggacgcc tacctgagct ggttgcgcac catgggggtg
     2221 ggctacgtgg tggcgtccga cgccccgccc gatttcagcg cggtcaacga ggcggccctg
     2281 ctggccggcg gacagtcggg cctgccccgg gtgttctccc aaggtcacgt aagcatctac
     2341 gccgtgccca acccgtcgcc gatggttacc ggtctgttcc cggcccgcgt gctcaccatc
     2401 accgagagcc aggtcgtgct ggccgtctcg cagcccgcca gctaccgcgt cgccgtgcgg
     2461 tactccccct actggcgatc acctaacgcg tgcgtcgagc ccgcgcccga cggcatgacc
     2521 aacatcgtcg ccgagagccc cggcatcgtg ccgctcacgt tccggatcaa cgcgggggcg
     2581 gccatgaagg ccctcaccgg ccagcccccc cactggtgcg gcggcgagga cacgtccgtg
     2641 ctcagcgccg gccggcccgg ctagggccgg cctcggacaa cccggggcct ctcaatgagc
     2701 ctcgcaaaat acccggagag agatgccggc cgttcatgct cgtggcgagc attttctgcc
     2761 aatagatgtg cagaccacga acggccggca tcgctttcgt agcgggctat tttgcgtggc
     2821 tcatcaggtg ccagggccgg ggaccggcac gtagggcgtc ggcagcagct cgatccacgt
     2881 cgagccagga tgcagcggca tcacggcgcc ggagggcagg tggagctgga gagcgtcgtt
     2941 gaacgtgggc cgggcccagc cgatctggaa catctgcccg tcgcgcagca cccatcccgt
     3001 gcccgttccg gtcaccaccg ggaggggatc ctcgttgtgg gcctggtcga agatcccggt
     3061 cccgtggatg gcgacctgga ggacgacgac gttggtggtg aacacctggc cgttgtcggt
     3121 cgtcacgtcg ggacttccgt cctgggcccg cacccagtgg ccgctcgggg cgtcccagcg
     3181 ccaggcgtcg gaggcggagt tggaatacgc catcttcacc ccgggcaccg gcgccccgcc
     3241 ggtcgccggc ggctgggccg cgtaggtgaa gagctggccg ggcgcggcgg gatgaccgcg
     3301 cttgagcccg gcggtgtaga agtcggccgt ggacccgtag acctggtcgg gcgcccgccg
     3361 gctcctgtcg acgtgaaatc cgggatcacc ggcgtcgtgg gaaaggcgca ccgcaccgcc
     3421 gtggtcgatc accggcgcga tctcgccgtt ggcggccccc gagtaggcga agatgccccc
     3481 gttgagctcg tgcagcaggt cggcgtcgac cgggcgggcc gagcggatcg ggcccatggt
     3541 cgccacgtcc tgcgactgga aggtcacgaa caggcgcgtg agcccgccct ccaccagctc
     3601 gaccgttacc acgtcggccc cgttgagccc gctctgcggg cgggcctggg cgacgttgtc
     3661 gatcttcacc gacagcgccg ggcgggccgc gttggggctg ttgttcacca gcagcccggt
     3721 cagcgggaac accggcgggg ccgtcgaggt tgtcggcgcc tggctggtgg acggcaccgc
     3781 gtgggccgcc ggcttgcggg ccgacttgcc gccgcacgcc gcggcgaaga cggctgcggc
     3841 tccgacagag acaccgaggg cgcgacgccg tgctgatcga gaaatggggc cctcccaggg
     3901 gagatggggc gaggccggtc cgtggccggc cgccggattc gcttccgacg ctaccggaat
     3961 ctatcggggc ggtggttgat ccccggcgca ccgcgccggt cagggccgaa cggccctcag
     4021 cacgtaggac cggggccggg cagcccgggc aggaggcggg cctggacgtc gatccggccc
     4081 ggacgcagga tggtcatctc cgtccaaccc tgggacgcgc ggg
//
LOCUS       DASWGG010000050         3138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2627424, whole genome shotgun sequence.
ACCESSION   DASWGG010000050 DASWGG010000000
VERSION     DASWGG010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2627424"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VF954_00835"
     CDS             <1..562
                     /locus_tag="VF954_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9529650.1"
                     /translation="HRQHSLPVVAPQMADRTIVVNGVAKSYAMTGWRVGWMIGPLDVV
                     KAATNLQSHATSNVSNVAQRAALAAVSGDLSAVAEMRAAFDRRRVRIHELLNDIPGVS
                     CMEPEGAFYAFPSFAGVLGRRLRGRRPATTVELAGIILDEAKVAIVPGEAFGAPGYAR
                     LSYALGDDDLVEGVTRIGKLLGEAEG"
     gene            complement(567..1349)
                     /locus_tag="VF954_00840"
     CDS             complement(567..1349)
                     /locus_tag="VF954_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9529651.1"
                     /translation="MIDAWDPARYERFKAERTAPFFDLLALTKPHPGALAVDLGCGTG
                     ELTAELHRHVQAAATTGIDRSANMLARAEAHAGGGLRFDRGDMAAFGGPPRYDIVAAN
                     AALQWLPDHVTTLARWSEGLNAGGQLAVQMPCNWDHPSHLLSAEVAAEQPFLDAFGGE
                     PPPDPVRGVLAPEAYAQLLDDLGYADQHVRLQVYGHRLPSADAVVDWVAGTSLTRFEE
                     RLTPELYALLVERYRERLREALGARAPYFYAFKRILLWARRP"
     gene            1414..2985
                     /gene="serA"
                     /locus_tag="VF954_00845"
     CDS             1414..2985
                     /gene="serA"
                     /locus_tag="VF954_00845"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX9529652.1"
                     /translation="MARILVTEELADRGLAILRDAGHAVDVRLGLSSEELLRAIAGAH
                     ALVIRSATQVTSVVLEAGRDLVVVGRAGIGLDNVDVAAATRLGVMVVNAPQSNILSAA
                     EQAMALLLAQARNVPQAHNSLTSGKWDRSRWEGVELHGKTMGIVGLGRVGALVAQRSL
                     AFGMRLVAYDPYVAGDRARQMGVELMSLEELMAEADFVSVHLPKTPETIGLVGRDLLA
                     KAKPGIRIINTARGGIVDEDALAEAIVDGRVAGAGLDVFATEPTTSSPLFELESVVVT
                     PHLGASTREAQDKAGVTIAEMVLLALAGEFVPFAVNVSASEASETVRPFLPLAEKLGR
                     IFASLVGGLPQTLDIEYQGALASYDTRILTLSVLKGVLGTNTDEPVSYVNAPQLASER
                     GLEVRETNSTSPHDYVNLITLRGGEHTVAGTLAGAKAEPRLVMVDGHVVEVPPANHML
                     VVRNDDRVGMIALVAGALAEAGVNISNMALGRSPSGGTALMVLATDQAAPAEVLTRLA
                     AEPGILGVHIVSAPA"
BASE COUNT          432 a         1101 c         1147 g          458 t
ORIGIN      
        1 gcaccgccag cactcgctcc cggtcgtggc tcctcagatg gccgatcgca cgatcgtcgt
       61 caacggcgtg gccaagagct acgccatgac ggggtggcgg gtgggatgga tgatcgggcc
      121 gctcgacgtc gtgaaagcgg cgaccaacct ccagtcccac gccacgtcca acgtgtccaa
      181 cgtggcccag cgggccgcgc tggccgcggt cagcggcgac ctctcggcgg tggctgagat
      241 gcgggccgcc ttcgaccggc gccgggtgcg catccacgag ctgctcaacg acatccccgg
      301 cgtgagctgc atggagccgg agggtgcttt ctacgccttc ccgtccttcg ccggggtgct
      361 cgggcgccgg ctgcgaggtc ggcgcccggc caccaccgtc gagctggccg ggatcatcct
      421 cgacgaggcc aaggtggcca tcgtgcccgg cgaggccttc ggtgcgcctg gctacgcccg
      481 cctgtcctac gccctcggcg acgacgacct ggtcgagggc gtgacccgga tcggcaagct
      541 gctgggcgaa gcggagggct gatcggtcag ggccgccggg cccacaagag gatccgtttg
      601 aacgcgtaga aatatggcgc ccgtgccccc agcgcctcgc ggagccgttc ccggtagcgc
      661 tccaccagga gggcgtacag ctccggggtg agccgttcct cgaacctggt caggctggtg
      721 ccggccaccc agtcgaccac ggcgtcggcc gacgggaggc ggtggccgta gacctggagg
      781 cggacgtgct ggtcggcgta cccgaggtcg tcgagcagct gggcgtaggc ctcgggcgcc
      841 agcacgccgc ggacgggatc gggcggtggc tccccgccga aggcgtcgag gaagggctgc
      901 tcggcggcca cctcggccga gaggagatgg ctggggtggt cccagttgca cggcatctgg
      961 actgcgagct ggcccccggc gttgagccct tcgctccagc gggccaacgt ggtcacgtgg
     1021 tcgggcagcc actggagggc cgcgttggcc gcgacgatgt cgtagcgcgg cgggccgccg
     1081 aaggccgcca tgtcgccccg atcgaaccgc aggcctccgc ctgcgtgggc ctccgcccgg
     1141 gccagcatgt tggcggaccg gtcgatgccg gtggtggccg cggcctggac gtggcggtgc
     1201 agctcggccg tcagctcacc cgtgccgcag ccgaggtcga ccgccagcgc gcccgggtgg
     1261 ggcttggtca aggcgagcag gtcgaagaag ggagcggtcc tctcggcttt gaaccgttcg
     1321 taccgggccg ggtcccatgc gtcgatcacc gggacatcac cgggacagtg tcgcgtggct
     1381 ggcggtcccc gacatcgtcc gcgaggatgc tgcatggccc gcatcctggt cacggaagag
     1441 ctcgccgacc gcggcctggc catcctgcgc gacgcgggtc acgccgtcga tgtccgcctg
     1501 ggcctgtcgt ccgaggagct gctccgggcg atcgccggcg cccacgcgct cgtgatccgc
     1561 tcggccacgc aggtcacgtc cgtcgtgctc gaggccggcc gcgacctggt ggtggtcggc
     1621 cgggccggca tcgggctgga caacgtggac gtcgccgcgg ccacccgcct cggcgtcatg
     1681 gtggtcaacg ccccgcagtc caacatcctc tcggccgccg agcaggccat ggccctgctc
     1741 ctggcccagg cccgcaacgt gccccaggcc cacaactccc tgacgtcggg gaagtgggat
     1801 cgcagtcgct gggagggtgt cgagcttcac ggcaagacca tgggcatcgt gggcctgggg
     1861 cgggtgggag cgctcgtggc ccagcggtcg ctggcgttcg ggatgcggct ggtcgcctac
     1921 gacccctatg tcgccgggga ccgggcccgg cagatgggcg tggagctgat gtcgctggag
     1981 gagctgatgg cggaggccga cttcgtgtcg gtgcacctgc ccaagacgcc cgagacgatc
     2041 gggctcgtcg gccgcgacct gctggccaag gccaagccgg gcatccgtat catcaacacc
     2101 gcccggggag gcatcgtgga cgaggacgcg ctggccgagg cgatcgtcga cggccgggtg
     2161 gccggtgccg gcctcgacgt gttcgccacg gagcccacga cgtcatcccc gctgttcgag
     2221 ctggagtcgg tcgtggtcac cccccacctc ggcgcgtcca cccgggaggc gcaggacaag
     2281 gccggcgtga ccatcgccga gatggtcctg ctggcgctgg cgggggagtt cgtgcccttc
     2341 gccgtgaacg tcagcgccag cgaggcgtct gagacggtcc gacccttcct gccgttggcc
     2401 gagaagctcg ggcgcatctt cgcctcgctg gtgggcgggc tgccccagac gctcgacatc
     2461 gagtaccagg gcgcactggc cagctacgac acccgcatcc tgacgctgtc ggtcctaaag
     2521 ggcgtgctgg ggaccaacac cgacgagccc gtgtcctacg tcaacgcgcc ccagctggcg
     2581 tcggagcgtg gcctggaggt gcgggagacc aacagcacct cgccccacga ctacgtcaac
     2641 ctcataaccc tccgcggcgg cgagcacacg gtggccggca ccctcgccgg cgccaaggcc
     2701 gagccgcgcc tcgtcatggt cgacggccac gtggtcgagg tgccgccggc caaccacatg
     2761 ctcgtggtcc gcaacgacga ccgggtgggc atgatcgccc tcgtggcggg tgcgctggcg
     2821 gaggccgggg tcaacatctc gaacatggcg ctgggccgct ccccgtccgg aggcacggcg
     2881 ctgatggtgc tggccaccga ccaggccgcg ccggccgagg tcctcacccg cctggccgcc
     2941 gagccgggca tcctaggcgt gcacatcgtc agcgcccccg cgtagaaagg gtcacccccg
     3001 cggcgggggc ctcggtggca caggtccgac ctgtctcatg ccctccgatg tcgttgtcac
     3061 acaccctctg tagattgaca gtatgttcgt ggagatcgtg gaccaggcga ccgaggccat
     3121 gcgctcggtg ctggcctc
//
LOCUS       DASWGG010000051        11635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2950666, whole genome shotgun sequence.
ACCESSION   DASWGG010000051 DASWGG010000000
VERSION     DASWGG010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2950666"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..550)
                     /locus_tag="VF954_00850"
     CDS             complement(74..550)
                     /locus_tag="VF954_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX9529653.1"
                     /translation="MPTPPKAGDQVLVLRVADDSFALPLWRVREVVAGPHITRLPTVP
                     SPVVGMFNLRGELVPAFDPSPLLDTPRQLPDPTRPPFVVVVDTEEGPAGLVAETLPSV
                     AVLGRELTAIDAPHALAVHAIRQSAVAVIDPGALLSGIAGPTTAAATLSPHRSPGP"
     gene            complement(556..2280)
                     /locus_tag="VF954_00855"
     CDS             complement(556..2280)
                     /locus_tag="VF954_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9529654.1"
                     /translation="MPPTLARTPTLARYRTRLVLGTVAVSLPVMLVLVAVLIAAGSHR
                     TIASARNFLSARAAHVSATVDDLVVTDMADCRFLAQRTASGLQADQVQPMLKQFVATH
                     DGFQGAEVVDLSGRPTASDDQTGAFDAAGQDWFREAASGREVLSPVYLDGSRIRWVTA
                     VPVLDASGRPAAVVLGDLRLTSLAPLLRHADLERSSVLLAFDADRRLVYSSTFPPGPG
                     DTVLLASGVLQTTVDTKDVRAALAGAAAASRLSDEHGHAVYSGYSRVSTTGWALVVRQ
                     AASEVDHPRNQLLVLGMLLAFAGTFAVLLFATAFAAFETGYMRRLVGEIRTAGEEVSL
                     NATDLSGASEELAATIVGQGGTVADTSTTMEELARSSATIATSIEAVATKAAETRDGL
                     VEASRDVASTSDRVMAVVARVADVSAILEQINDIADQTNLLSLNAAIEAARAGEEGRG
                     FAVVADEVRRLAERSKDLASSISGLMDATQSETHEMVMAMEKRARQITRDVTLLEDVA
                     TSTAEISITTQQQQVATQQVVDALSQLMQSSRQVTDTAQQIASAASTLAALSAQLDRA
                     AAGTAARF"
     gene            2474..6070
                     /gene="dnaE"
                     /locus_tag="VF954_00860"
     CDS             2474..6070
                     /gene="dnaE"
                     /locus_tag="VF954_00860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX9529655.1"
                     /translation="MGRSGDSFVHLHVHTEFSMLDGASRVAEVVAGAAADGQPALGIT
                     DHGNMYGVLDFYQECKDHGINPVIGTEAYMAAESRRERPVRRGRIDDTGGDAAAGEKL
                     YYHLTLLAESDQGYRNLLKLSSAAYLEGYYYKPRVDWELLERHRGGIIATTGCLGGVV
                     LQALLRGDQEGAARLAARLQDIFGRDNLFVELQDHGLAKQHKTNPQLIEIARRIGAPL
                     LATNDSHYTRRTDAVAHDALLCVQTGASIDDPNRFKFDGDQHYLKTAAEMRALFSGWE
                     EACDNTLWIAERARVEIAFGHPQLPEVSLPDGFTSADAYLRHLTYAGAEARYGTPLPG
                     AVVERLDHELGVIADMGFSAYFLVVWDLIRHAKEGGIRVGPGRGSAAGCCVAYCLRIV
                     ELDPLRYGLIFERFLNPGRKSMPDIDMDFDERYRAEMIRYAAERYGWDHVAQIVTFST
                     IKARAAVRDAARVLGYPYAVGDRIAKAMPPLVMGRDTPLYACLEEHPKYHDGFKMATE
                     LRDMYATDPDAERVINVARGLEGLRRQDGIHAAAVVITRDPLTEYLPIQRKPEPGGNV
                     DDAPIVTQYEMHGVEDLGLLKMDFLGLRNLSVIERALDLIEESTGNRPDIDGVPLDDD
                     ATLAMLRRGESIGVFQLEGGPMRALERSLAPTSFDDVAALVALYRPGPMAANMHNDYA
                     DRKNGRKPITYQHPEMEEILGDTYGLMIYQESLMRVAQRFAGYSLEEADNLRKACGKK
                     IRELMAKERERFVASCEATGYPRALGTALFDIIEPFADYAFPKAHAYGYGLVSYQTAW
                     LKANHPVEYFAALLTSVRDDKDRTAVYLAECRAMGIKVLVPDINASASEFAARRPTEA
                     GRAEGSRGHIPFGLSAVRNVGEGLVAQVVAEREANGPFRDFYDFCERVDPNVLNKRTI
                     ESLVKAGGFDSLGHPRKGLCLVFEQIVDRILARRRERELGVMSLFGDVEDAGAGGFDD
                     SRVPIPDDEFDKAQRLAFEKEMLGLYVSDHPLMGVEAALRRLTDTTIGELREAASAVA
                     GPAGGSGRDSEVKVVGGVVTNLVRKYTKRGDLMATFILEDLESSIEAWVFPRTMQEFG
                     SLLTDDAIVCVKGRVDAREEPVKLVVMDVKRPELVSDGGPPVRVSLPLGALTDSLVRG
                     LKHILAEHPGDSPVYLHVGDKILRLPDEFNVNARRGLLGELKVLLGPHALVA"
     gene            6225..6899
                     /locus_tag="VF954_00865"
     CDS             6225..6899
                     /locus_tag="VF954_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529656.1"
                     /translation="MAMDVETKDCTALSDAELAEMADICTDGPSGFDVGFLSKQREDW
                     VLITQVRDGNKLHGFSFCTLERIGGTPSLLIGLASVKRSSKRDTALRALMSDQYRRAV
                     LAFPDEDVLVGTRMSHPAAFQAYKGLEDVVPRPGHKASGEERAWGRRLAKRFGAEGRI
                     DDRTFALTGDGLVCGLLDHESLRPVGVEPDMGAFFGPLDTSRRDTLVAFGWAMAEDLA
                     SRASVR"
     gene            6936..7949
                     /locus_tag="VF954_00870"
     CDS             6936..7949
                     /locus_tag="VF954_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9529657.1"
                     /translation="MKVGLVLGAGGTVGLAYHSGALHALEDIGGFRPQDADLIVGTSA
                     GSVIGSYLRTGWTTTDFWQFALGTHPHMAPLGRAPRAEPLVPTAAPDLPGRDLPGPEF
                     LAPAYRGPVDFVRRSVGSAYVLSRSAVRVPVPVVPKPLRAAFPAGIFVMSEGRRRFAK
                     ELPAAWPPEPLWLCAVDIVSGRRIVLGSRGSPPMTLQKAVMASCAIPGVYPPVPLGRR
                     VLIDGGVHSTTNLDLAVRAGCEMIIAVAPMAYDSRLAPGWVGRAARRVPMQSLNREVA
                     LARRRGVRVLLVRPSATEVGLHGYRLMRSDGLDLVARAAFGATRLLVRTDRFRRALDD
                     LAA"
     gene            complement(8145..8825)
                     /locus_tag="VF954_00875"
     CDS             complement(8145..8825)
                     /locus_tag="VF954_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529658.1"
                     /translation="MVAVNTTVRTRTAEPGAPVTLLMLASGPRGGIGVDLDSGAMVRA
                     RYEQPVGRVVRPFDVVTTTVADTQDEPDPTRPEALVVNAPLRPAGLMRGRTIRRMLRR
                     LVQPAGKHLLGVPGPALPFWTMTAAGPSVVLVEPEHGPDIAALAGTRPGGSNPRCQFR
                     WGGQDLDLPLQDRHLQSVMARTGQTRVSGPGLAHAIGGPPAHLLVTLTPPFGGHCYKV
                     VAGVLPRP"
     gene            complement(8979..10979)
                     /locus_tag="VF954_00880"
     CDS             complement(8979..10979)
                     /locus_tag="VF954_00880"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9529659.1"
                     /translation="MNRLADETSPYLRQHRDNPVDWYPWGEEAFARAREQDLPILLSV
                     GYSACHWCHVMAHESFEDPGTAAIMNRLFVNVKVDREERPDVDAVYMEAVQALHGSGG
                     WPMTVFLTPDRRPFFAGTYFPPRGRGGSAGFPQVLEAVADAWEHRRDEVVDQAGQLAD
                     AIRARTELPAGGPGHALDSTLLEQAYAGLRAAHDDEQGGFGRAPKFPQPAMVDFVVRA
                     AARNRSPETLAMLATTLDAMASGGMYDHLGGGFARYSVDAFWMVPHFEKMLYDQAGLL
                     RVYLHAWQLTGEPRWSQVAGEIVGYVLRDLRGAEGGLCSAEDADSEGEEGRFYLWSMD
                     EIETVLGADLAAEAGEWYGVTATGNFEGRNILHRPRRGDLIRPARVEEARERLFRHRL
                     ARVRPGLDDKVITEWNAMLLASLAEAAGALARPDWQDAAVALGEFLLAELRRPDDGRW
                     LRSWQGGRARHLAYAADYAWLVDAFTRLAELTGSRRWLEEASTVADQMVSLFWDEERG
                     GLFTTGFDGEQLIVRTKDLFDGAIPSANSVAAVALLRLGALTGSARFGERAEAILAML
                     AEPLAHSPTAFAHVLAAVDLLLTGATEVVVAGDRPDLVAAARRHFLPNGVVAWGEPWP
                     SPLWEGRAPGRAYVCRNYSCLLPATTVEELETQLRQADGQDG"
     gene            complement(11079..>11635)
                     /locus_tag="VF954_00885"
     CDS             complement(11079..>11635)
                     /locus_tag="VF954_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9529660.1"
                     /translation="RILIEDGLDGVSLRRVAGSLGVTAPALYAYVKDKHDMLQGIADI
                     ELEDLQDRFGEVEGLDPEDRLHDMAMTYVNFARHNSSVFRAIFLVHPELAMRTADDEP
                     SMTRVFQAGSAPIQELDQRGQLGETDAETAALAFWTAVHGAATVMASGSYLDLAERER
                     AAETVINSVLSGLHGPSARDAGAA"
BASE COUNT         1665 a         4102 c         4240 g         1628 t
ORIGIN      
        1 aggtggccga gcctggtctc cgcctcctgg ccgaacagct cgagcagctc gcgctggaga
       61 tcccgcgcca cggctagggg cccgggctgc gatgggggga aagggtggcc gccgcggtgg
      121 tcggccccgc gatcccggac agcagggcgc ccgggtcgat cacggcgacc gccgactgcc
      181 tgatggcgtg gacggcgagc gcgtggggtg catcgatcgc ggtcagctcg cgcccgagga
      241 ccgccaccga tggcagcgtc tccgcgacca gcccggccgg tccctcctcg gtgtccacga
      301 cgacgacgaa gggcgggcgg gtcgggtcgg gcagctgccg cggcgtgtcg agcagcggac
      361 tcgggtcgaa ggccggcacg agctcgcccc gaaggttgaa catgccgacg accggagagg
      421 gcacggtcgg caggcgcgtg atgtgtggac cggcgaccac ctcgcgcacc cgccacagcg
      481 gcagggcgaa gctgtcgtcg gctacgcgca ggaccaggac ctggtcgcca gccttcggcg
      541 gggtgggcat cacgatcaga accgggccgc ggtccccgcc gcggcccggt ccagttgggc
      601 agacagcgcg gcgagcgtgc tggccgccga ggcgatctgc tgcgcggtgt cggtgacctg
      661 gcgactggac tgcatgagct ggctcagggc gtcgacgacc tgctgcgtcg ccacctgctg
      721 ctgctgtgtc gtgatgctga tctcggctgt ggaggtggcc acgtcctcga gcagggtcac
      781 gtcccgcgtg atctgcctcg ccctcttctc catcgccatc accatctcgt gggtctcgga
      841 ctgggtggcg tccatgaggc cgctgatgct ggaggccagg tccttggaac gctcggcgag
      901 acgccggacc tcgtcggcca cgacggcgaa gccccggcct tcctcgccgg cccgggccgc
      961 ttcgatggcg gcgttgagcg acaacaggtt ggtctggtcg gctatgtcgt tgatctgctc
     1021 caggatggcg gacacgtccg ccaccctggc caccacggcc atcacccgat cgctggtcga
     1081 agccacgtcg cggctggcct cgaccagccc gtcgcgcgtc tcggccgcct tggtcgcgac
     1141 cgcttcgatg ctggtggcga tggtggccga ggaccgggcc agctcctcca tggtggtcga
     1201 ggtgtcggcg acggtcccac cctgcccgac gatcgtggcg gccagctcct cggatgcgcc
     1261 cgagaggtcg gtggcgttga gcgacacctc ttcacccgcg gtgcggatct cgcccacgag
     1321 ccggcgcatg tagccggtct cgaaggcggc gaacgcggtg gcgaacagca ggactgcgaa
     1381 ggtgcccgcg aaggcgagaa gcatgcccag cacgaggagc tggttgcgcg ggtggtcgac
     1441 ctcgctggcc gcctggcgca cgacgagtgc ccagccggtg gtggagaccc ggctgtagcc
     1501 gctgtagacg gcgtggccgt gctcgtcgga caggcggctt gcggccgcgg ccccggccag
     1561 ggcggcccgc acgtccttgg tgtcgaccgt ggtctgcaac acgccgctgg cgagcaggac
     1621 ggtgtcgccc ggccccggcg ggaacgtcga gctgtacacg aggcgccggt cggcgtcgaa
     1681 agccagcagc accgaggagc gctccaagtc ggcgtggcgc agcaggggag cgagcgaggt
     1741 caaccggagg tcgcccagga ccaccgcggc cggccggccg gacgcgtcga gcaccggtac
     1801 cgcggtcacc cagcggatgc ggctcccgtc caggtacacc gggctgagga cctcgcgtcc
     1861 cgacgccgct tcccggaacc agtcctggcc ggccgcgtcg aacgcgccgg tctggtcgtc
     1921 gctcgccgtc ggccttcccg acaggtcgac cacctctgcg ccctggaaac catcgtgggt
     1981 ggcgacgaac tgcttgagca tcggttggac ctggtccgcc tgcaggccgc tggcggtccg
     2041 ctgggcgagg aagcggcagt cggccatgtc cgtcacgacg agatcgtcga cggtcgcaga
     2101 cacgtgcgcg gcgcgggcgg acaagaagtt gcgcgccgac gcgatcgtgc ggtgcgaccc
     2161 ggccgcgatc agcacggcca cgaggaccag catgacgggg agggagaccg cgaccgtccc
     2221 gaggacgagc cgggtgcggt acctggcgag agtcggtgtg cgggcgaggg tcggaggcat
     2281 tgatcgtccc gaaaggggta ggccgcagcc taattggtgg ctttcggccg aagcgcgacc
     2341 ggtgccggtc ggggccggtt gcgctaccct ccaggaacta caggtgtgta actcgtccac
     2401 aggctgtgga caaccgggtc tggacaacgg gcgccgtgcg tggcagaggg cgcggcagag
     2461 gggaggagca gtggtgggcc ggagcggcga ttcgttcgtt cacctccacg tgcacaccga
     2521 gttctccatg ctcgacggtg cgtcccgggt ggccgaggtg gtcgccgggg ccgccgccga
     2581 cggccagccc gcgctcggca tcaccgatca cgggaacatg tacggggtcc tcgacttcta
     2641 ccaggagtgc aaggaccacg ggatcaaccc cgtcatcggg accgaggcct acatggcggc
     2701 cgagagccgc cgggagcggc ccgtgcgccg tgggcgcatc gacgacaccg gaggcgatgc
     2761 cgcggccggc gagaagctct actaccacct gacgctcctg gcggagagcg accaggggta
     2821 ccgcaacctc ctgaagctgt ccagcgcggc ctacctcgag ggctactact acaagccccg
     2881 ggtcgactgg gagctgctgg agcgccaccg cggcggcatc atcgccacca ccggatgcct
     2941 cggtggcgtg gtcctccagg ccctcctccg gggggaccag gagggggccg cccggctggc
     3001 cgcccgcctc caggacatct tcggccgcga caacctcttc gtcgagctcc aggaccacgg
     3061 gctggccaag cagcacaaga ccaatccgca gctgatcgag atcgcccgcc ggatcggcgc
     3121 ccccctgctg gccaccaacg acagccatta cacccggcgc accgacgccg tcgcccacga
     3181 cgccctcctg tgcgtgcaga cgggcgcgtc catcgacgac cccaaccggt tcaagttcga
     3241 cggggaccag cactacctga agacggcggc cgagatgcgg gcattgttct cgggatggga
     3301 ggaggcctgc gacaacaccc tgtggatcgc cgagcgggcc agggtggaga tcgccttcgg
     3361 ccatccgcag ctccccgagg tctcgctgcc ggacgggttc acgtcggccg acgcctacct
     3421 acgccacctg acctacgcgg gtgccgaggc ccggtacggg acgccgcttc ccggcgcggt
     3481 ggtcgagcgc ctcgaccacg agctcggcgt catcgccgac atgggcttca gcgcctactt
     3541 cctggtggtg tgggacctca tccgccacgc caaggagggc ggcatacggg ttgggcccgg
     3601 ccggggctcg gcagccgggt gctgcgtggc ctactgcttg cgcatcgtgg agctcgaccc
     3661 gctccgctac gggctgatct tcgagcgctt cctgaacccg gggcggaagt cgatgcccga
     3721 catcgacatg gacttcgacg agcggtaccg ggccgagatg atcaggtacg cggccgagcg
     3781 gtacggctgg gaccacgtgg cccagatcgt caccttctcg accatcaagg cccgggccgc
     3841 cgtccgggac gccgcccggg tgctgggcta cccgtacgcg gtcggggacc gcatcgccaa
     3901 ggccatgccg ccgctcgtga tggggcggga caccccgctg tacgcgtgcc tggaggagca
     3961 ccccaagtac cacgacggct tcaagatggc caccgagctg cgcgacatgt acgcgaccga
     4021 tcccgacgcc gagcgggtca tcaacgtggc ccggggcctg gagggcctcc gccgccagga
     4081 cggcatccat gccgctgcgg tcgtgatcac ccgcgacccg ctgacggagt acctccccat
     4141 ccagcgcaag ccggagcccg gcggcaacgt cgatgacgcg cccatcgtca cccagtacga
     4201 gatgcacggg gtggaggacc tcggcctgct caagatggac ttcctcgggc tgcgcaacct
     4261 cagcgtcatc gagcgggcgc tcgacctcat cgaggagtcc accggcaacc ggcccgacat
     4321 cgacggcgtg ccgctcgacg acgatgcgac actggccatg ctgcggaggg gcgagagcat
     4381 cggggtgttc cagctcgagg ggggacccat gcgggcgctc gagcgctcgc tcgcgcccac
     4441 cagcttcgac gacgtggccg ccctggtcgc cctgtaccgg ccggggccca tggcggccaa
     4501 catgcacaac gactacgccg accgcaagaa cggtcgcaag ccgatcacct accagcatcc
     4561 ggagatggag gagatcctcg gcgacacgta cgggctcatg atctaccagg agagcctgat
     4621 gcgcgtggcc cagcgtttcg ccggctactc gctcgaggag gcggacaacc tccggaaggc
     4681 ctgcgggaag aagatacgcg agctcatggc caaggagcgg gagcggttcg tggcgagctg
     4741 cgaggcgacg ggctacccgc gggcgctggg gaccgcactg ttcgacatca tcgagccctt
     4801 cgccgactac gccttcccca aggcccacgc ctacgggtac ggcctggtca gctaccagac
     4861 cgcgtggctc aaggccaacc acccggtcga gtacttcgcc gccctgctca ccagcgtcag
     4921 ggacgacaag gaccgcacgg cggtgtacct ggcggagtgc cgggcgatgg gcatcaaggt
     4981 cctcgtgccc gacatcaacg cctcggcgtc ggagttcgcc gcccgccggc ccaccgaggc
     5041 cgggcgcgcc gaggggtccc ggggtcacat cccgttcggg ctgtcggcgg tccgcaacgt
     5101 gggcgagggc ctggtcgccc aggtggtggc cgagcgcgag gccaacggcc cgttccgcga
     5161 cttctacgat ttctgcgagc gggtcgaccc caacgtgctc aacaagcgca cgatcgagtc
     5221 gctcgtcaag gccgggggct tcgactcgct cggccatccc cgcaagggcc tgtgcctcgt
     5281 cttcgagcag atcgtggacc ggatcctggc ccggcgccgg gagcgcgagc tgggtgtcat
     5341 gagcctcttc ggcgatgtcg aggacgccgg cgcgggaggt ttcgacgact cccgggtgcc
     5401 gataccggac gacgagttcg acaaggccca gcgcctggcc ttcgagaagg agatgctcgg
     5461 cctctacgtg agcgatcatc cgctgatggg cgtggaggcg gccctgcgcc gcctcaccga
     5521 cacgacgatc ggcgagctgc gcgaggcggc gagcgcagtg gccgggccgg cgggaggctc
     5581 cgggcgggac agcgaggtca aggtggtcgg cggggtcgtg accaacctgg tgcgcaagta
     5641 cacgaagcgc ggtgacctga tggccacctt catcctggag gacctcgagt cgtcgatcga
     5701 ggcatgggtc ttcccgcgca ccatgcagga gttcgggtcg ctgctcaccg acgacgccat
     5761 cgtgtgcgtg aagggacgcg tcgacgcccg ggaggagccc gtcaagctgg tcgtgatgga
     5821 cgtgaagcgg cccgagctcg tgtccgacgg tgggcctccg gtgcgggtgt cgctgccgct
     5881 cggtgccctg accgactcgc tcgtccgggg gctcaagcac atcctggcgg agcatccggg
     5941 ggactcgccc gtgtacctcc atgtcggcga caagatcctg cgcctgcccg acgagttcaa
     6001 cgtcaacgct cgcaggggcc tcctcggcga gctgaaggtg ctcctcggcc ctcacgccct
     6061 cgtcgcctga cccgaccagg ggggttgggg cccccacgct ccggaggagg gcggggtcgg
     6121 ggccccgccc gggccgacag cacggcgtgg accggattgg tgcccatccc ctaatctgga
     6181 acttggccgc ccggtcaaga tgaggtaagg gacgcgcacg acccatggcg atggacgtcg
     6241 agaccaagga ctgcaccgct ctcagcgacg ccgaactggc agagatggcc gacatctgca
     6301 ccgacgggcc gtccggcttc gacgtcggct tcctgtccaa gcagcgcgag gactgggtgc
     6361 tcatcaccca ggtccgcgac ggcaacaagc tgcacggctt ctcgttctgc acgctcgaac
     6421 gcatcggtgg gaccccctcg ctgctgatcg gcctggcgtc ggtgaagcgc agctccaagc
     6481 gcgacacagc gctgcgggcg ctgatgagcg atcagtaccg gcgggcggtg ctcgccttcc
     6541 cggacgagga cgtgctggtc gggacgcgta tgtcgcatcc ggccgccttc caggcctaca
     6601 agggcctcga ggacgtcgtg ccccgccccg gccacaaggc cagcggtgag gagcgagcgt
     6661 gggggcggcg gctggccaag cggttcgggg ccgaggggcg catcgacgac cggaccttcg
     6721 cgctcacggg agacgggttg gtgtgcgggc tgctcgacca cgagtcgctg agacccgttg
     6781 gcgtcgagcc cgacatgggg gcgttcttcg gcccgctcga cacgagccgc cgcgacacgc
     6841 tggtcgcgtt cggctgggcg atggcggagg acctcgcctc gcgggcatct gtccggtaaa
     6901 ggttcgcggg aagcgggggt catggaagcg cgtcggtgaa ggtcgggctg gtgctcggtg
     6961 ccgggggcac cgtcggcctc gcgtaccact cgggtgcgct ccatgccctg gaggacatag
     7021 gtgggttccg tccccaggac gccgacctga tcgtcgggac gagcgcgggc agcgtcatcg
     7081 gctcctatct ccgcacgggc tggaccacga ccgacttctg gcagttcgcc ctcggcacgc
     7141 acccccacat ggcgccgctc ggccgggcgc cgcgggccga gccgctcgtg ccgacggcgg
     7201 cgcccgacct ccccgggcgc gacctcccgg ggcctgagtt cctcgcgccc gcgtaccggg
     7261 ggccggtcga cttcgtgcgg cgctccgtcg gctcggcgta cgtcctcagc aggtcggcgg
     7321 tgcgcgtgcc cgtgccggtg gtcccgaagc cactgcgggc cgctttcccc gccgggatct
     7381 tcgtgatgtc cgaggggcgg cgccgcttcg ccaaggagct gcccgccgcc tggccgcccg
     7441 agcccctctg gctctgtgcc gtcgacatcg tcagcggccg gcgcatcgtg ctcggcagcc
     7501 ggggctcgcc cccgatgacc cttcagaagg cggtcatggc gtcctgcgcc atcccgggcg
     7561 tctatccgcc cgtgccgctc ggccggcggg tcctgatcga cggcggcgtc cactcgacga
     7621 ccaacctcga tctcgccgtc cgcgccggct gtgaaatgat catcgcggtg gccccgatgg
     7681 cctacgacag ccggttggcg cccggctggg tggggcgcgc cgcccggcgc gtgccgatgc
     7741 agtcgctcaa ccgggaggtg gcgctcgccc gccggcgagg cgtgcgggtg ctgctcgtgc
     7801 ggccttccgc cacggaggtg ggcctgcacg gctaccgcct catgcgcagc gacgggctcg
     7861 acttggtggc cagggcggcc ttcggagcga cccgcctcct ggtgcgcacg gatcggttcc
     7921 gccgggcgct cgacgatctg gccgcctgaa ggcgcggtcg gggcggttcg atcccttgtg
     7981 atcgcattct gttgacactt atgccagcat agccggcata agtgtcaaca gaatacccag
     8041 gggtggtaag gccagccacg aaaggtgggg atggtgctgt ggcgggtagc gccgcagcac
     8101 ctggcctata gaaaccgcgg taagcgtcgc ccggggccgg gcgactaagg ccggggcagg
     8161 acccccgcga ccaccttgta gcaatggccg ccaaagggcg gcgtcagcgt gacgagcagg
     8221 tgggcgggcg ggcccccgat ggcgtgggcc agcccggggc cgcttacccg ggtctggccg
     8281 gtgcgggcca tgaccgactg gaggtggcga tcctggaggg gaaggtcgag gtcctggccg
     8341 ccccaccgga actggcagcg ggggttcgac ccacccggcc gggtgcccgc cagggcggcg
     8401 atgtccggcc cgtgctcggg ctcgacgagc accaccgacg ggcccgcggc cgtcatcgtc
     8461 cagaagggca gcgccggccc gggcaccccg agcaggtgct tgccagccgg ctggacgagg
     8521 cgccgcagca tccggcggat cgtccggccg cgcatcagtc cggcgggccg gagcggggcg
     8581 ttgacgacca gcgcctcggg acgggtcggg tcgggctcgt cctgggtgtc ggcgacggtg
     8641 gtggtgacca cgtcgaaggg ccgcacgacc ctgccgaccg gctgctcgta acgggcccgg
     8701 accatggcac ccgagtcgag gtccacgccg atgccgcccc gcgggcccga ggcgagcatg
     8761 aggagcgtga ccggtgcccc gggctcggcc gttcgggttc ggacggtggt gttgaccgcg
     8821 accacgtccc ccatgctggt cggggccggg gcgcgggagg tggatccccc cggggagccg
     8881 agcggcccgg gagggaccgg tggtcgccac ggggccgaag ggaccggcga gccccccggc
     8941 acgggcgttg cttcagtagc tgggcccacg cgcgcagctt atccgtcctg tccgtcggcc
     9001 tgccggagct gtgtctcgag ctcctccacg gtcgtggccg gcagcaggca gctgtagttg
     9061 cggcacacgt aggctcgccc cggcgcccgt ccctcccaca gcggcgatgg ccagggctcc
     9121 ccccacgcca ccaccccgtt ggggaggaag tggcgccggg cggccgccac cagatcgggg
     9181 cgatcgcccg ccaccaccac ctcggtggcg ccggtgagca gcagatcgac cgcggccagt
     9241 acgtgggcga aggccgtggg tgagtgggcg agcggctcgg ccagcatggc gagaatggcc
     9301 tcggcccgct ccccgaaccg cgccgagccc gtgagcgcgc ccaggcggag cagcgccacc
     9361 gccgcgaccg agttggcgga cggaatggcc ccgtcgaaca ggtccttggt gcgcacgatc
     9421 agctgctcgc cgtcgaaccc ggtcgtgaag agcccgcccc gctcctcgtc ccagaacagc
     9481 gacaccatct gatcggccac cgtgctggcc tcctccagcc accggcgcga gccggtgagc
     9541 tcggccagcc gggtgaaggc gtcgaccagc cacgcgtagt cggcggcgta ggccaggtgg
     9601 cgggcccgac cgccttgcca tgagcgcagc caccggccgt catccgggcg gcgcagctcc
     9661 gccagcagga attcgcccag cgccacggcg gcgtcttgcc aatccggccg tgcgagcgcg
     9721 cccgccgcct cggccagcga cgccaggagc atggcgttcc actcggtgat gaccttgtcg
     9781 tcgagacccg gccggacccg tgcgaggcgg tggcggaaca gccgctcgcg ggcctcctcc
     9841 acccgcgccg gccggatcag gtcgccccgg cggggccggt gcaggatgtt gcgcccctcg
     9901 aagttcccgg tcgcggtgac gccgtaccat tcgccggcct cggcggcgag gtcggccccg
     9961 aggaccgtct cgatctcgtc catcgaccac aggtagaacc ggccctcctc gccctccgag
    10021 tcggcgtcct cggcggagca aaggccgcct tcggcgccgc gcaggtcgcg cagcacgtag
    10081 cccacgatct ctcccgccac ctgcgaccac cggggctcgc cggtcagctg ccaggcgtgg
    10141 aggtagaccc ggagcaggcc ggcctggtcg tagagcatct tctcgaagtg cggcaccatc
    10201 cagaaggcgt cgaccgagta ccgggcgaac ccgccaccca ggtggtcgta catccccccc
    10261 gacgccatgg cgtccagggt ggtcgccagc atcgccaggg tctcggggct gcggttgcgg
    10321 gccgcggctc ggaccacgaa gtcgaccatg gccggctggg ggaacttggg cgcccggccg
    10381 aacccgccct gctcgtcgtc gtgggcggcc cgcaacccgg cgtaggcctg ctccagcaag
    10441 gtcgagtcga gggcatgccc ggggccgccg gccggcagct ccgtgcgcgc ccggatggcg
    10501 tcggcgagct gcccggcctg gtccacgacc tcgtcccgcc ggtgctccca cgcgtcggcc
    10561 accgcctcga ggacctgcgg gaagccggct gacccgcccc gcccccgggg cgggaagtag
    10621 gtgccggcga agaacggccg gcggtccggg gtgaggaaca ccgtcatcgg ccagccgcca
    10681 ctgccgtgca gggcctggac cgcctccatg tacacggcgt cgacgtcggg ccgctcctcc
    10741 cggtcgacct tgacgttgac gaagaggcgg ttcatgatcg ccgccgtccc ggggtcctcg
    10801 aaggactcgt gggccatgac gtggcaccag tggcaggccg agtagccgac ggagagcagg
    10861 atgggcaggt cctgctccct ggcccgggcg aacgcctcct cgccccacgg gtaccagtcc
    10921 accgggttgt cccggtgctg gcggaggtag gggctggtct cgtcggcgag tcggttcatg
    10981 gtccggtcct cggcctctcc gtcggcgggg ccctcgtttg ctacgacgcg acccattgtg
    11041 accaccggag cgcccggggt gccgccgccg tctgggtctc aggccgcccc ggcgtcgcgg
    11101 gcggacgggc cgtggagccc ggacagcacg ctgttgatca ccgtctcggc ggcgcgctcg
    11161 cgctccgcca ggtccaggta gctgcccgac gccattaccg tggccgcccc gtggaccgcc
    11221 gtccagaagg ccagggcggc ggtctcggcg tccgtctcgc ccagctggcc gcgctggtcc
    11281 agctcctgga tcggcgccga gccggcctgg aagacacggg tcatcgacgg ctcgtcgtcc
    11341 gccgtccgca tggccagctc gggatgcacc aggaagatgg cccggaagac ggaggagttg
    11401 tggcgggcga agttgacata cgtcatggcc atgtcgtgca ggcggtcctc ggggtcgagc
    11461 ccctccacct cgccgaagcg gtcctggaga tcctcgagct cgatgtcggc gatgccctgg
    11521 agcatgtcgt gcttgtcctt gacgtacgcg tacagcgccg gcgcggtcac gcccagcgat
    11581 ccggcgaccc gccgaagcga caccccgtcg aggccgtcct cgatcagtat ccggc
//
LOCUS       DASWGG010000052         1519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1016337, whole genome shotgun sequence.
ACCESSION   DASWGG010000052 DASWGG010000000
VERSION     DASWGG010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016337"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="VF954_00890"
     CDS             complement(<1..1329)
                     /locus_tag="VF954_00890"
                     /inference="COORDINATES: protein motif:HMM:NF023702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX9529661.1"
                     /translation="MATLAELARSHTRLTADDAAHLQRLAGAWGMLADLCFADLLLLA
                     PVAGEEGHRFVVLAQVRPTTDQTLYRDDMVGTVVDEVERPLIARVWRLGEIGVGDAAV
                     IGQTGERANVQAIPVRHRGKVVALVTREAATTTGRRLGELERVYFEIFDRFARMLYEG
                     NFPFKGGAQVQEETPRVGDGVLFLDTESTVRYASPNAVSSLHRMGIHANAQGQRLSDI
                     GLDDDAVRTAFAARAPVTEELEWGDTSILLQVIPLLEEGNPNGAILLMRDVSELRRRD
                     RLLLSKDATIREIHHRVKNNLQTIASLLRLQGRRLSAPEAKQALEESVRRIRSIALVH
                     ETLAYEAGNTVPFGEIIRPLVRVVEDGLVSPDRHIRFTVEGDPGDLPAEVATPLAVVL
                     TELLQNAVEHGFPDNGSGHTREGFVTVRLVREGDEAVVEVVDNGVGLPPGF"
     gene            complement(1336..>1519)
                     /locus_tag="VF954_00895"
     CDS             complement(1336..>1519)
                     /locus_tag="VF954_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529662.1"
                     /translation="PAPPLLARFHQAGAPLTTASDAHELALVADGTDRLRALLADAGV
                     TELCGFERRRPSPRAV"
BASE COUNT          202 a          535 c          577 g          205 t
ORIGIN      
        1 gaagccgggc ggcagcccga cgccgttgtc gaccacctcg accacggcct cgtcgccctc
       61 ccgcaccagg cgcacggtga caaacccctc ccgggtgtgg cccgacccgt tgtcggggaa
      121 gccgtgctcg acggcgttct ggaggagttc ggtcagcacc accgccagcg gggtggccac
      181 ctcggccggc aggtcgccgg ggtcgccctc gaccgtgaag cggatgtggc ggtcggggga
      241 gacgagcccg tcctccacca cccgcaccag cggccggatg atctcgccga acggcacggt
      301 gttgccggcc tcgtaggcca gcgtctcgtg caccagggcg atcgaccgga tccggcgcac
      361 cgactcctcc agcgcctgct tggcctccgg tgccgacagc cgccgcccct ggagccgcaa
      421 cagcgaggcg atcgtctgga ggttgttctt cacccggtgg tggatctccc ggatggtggc
      481 gtccttggac aggagcaacc ggtcccgccg gcgcagctcc gacacgtccc gcatgagcag
      541 gatcgcgccg ttggggttgc cctcctccag cagcgggatc acctggagga ggatcgacgt
      601 gtcgccccac tccagctcct cggtgaccgg cgcccgggcg gcgaaggcgg tgcgcaccgc
      661 gtcgtcgtcg aggccgatgt cgctcaaccg ctgcccctgg gcgttggcgt ggatccccat
      721 ccggtgtaac gagctgacgg cgttgggact ggcgtagcgg acggtcgact cggtgtcgag
      781 gaacaggacg ccgtcgccga cccggggcgt ctcctcctgc acctgggccc cgcccttgaa
      841 ggggaaattg ccctcgtaga gcatgcgggc gaagcggtcg aagatctcga agtagacccg
      901 ctccagctcg ccgaggcgcc ggccggtggt ggtggccgcc tcccgggtga ccagcgccac
      961 caccttgccc cggtggcgca ccgggatggc ctgcacgttg gcccgctcac cggtctggcc
     1021 gatgacggcg gcgtcgccga cgccgatctc gccgagacgc cacacccggg cgatgagcgg
     1081 ccgctcgacc tcgtcgacga cggtgccgac catgtcgtcg cggtacagcg tctggtcggt
     1141 ggtcgggcgc acctgggcca gcaccacgaa ccggtgcccc tcctcgccgg ccaccggggc
     1201 gaggaggagg aggtcggcga agcagaggtc ggccagcatg ccccaggcgc cggccagccg
     1261 ttggaggtgg gcggcgtcgt cggcggtcag ccgggtgtgg gaacgggcca gctcggcgag
     1321 ggtggccatg gctcctcaga cggccctggg ggacgggcgg cgccgctcga agccgcacag
     1381 ttcggtgacg ccggcgtcgg ccaggagggc ccggagccgg tcggtgccgt cggccaccag
     1441 cgccagctcg tgggcgtcgg aggcggtggt gagcggggcg ccggcctggt ggaagcgggc
     1501 caggagcggc ggggcgggg
//
LOCUS       DASWGG010000053         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2953386, whole genome shotgun sequence.
ACCESSION   DASWGG010000053 DASWGG010000000
VERSION     DASWGG010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2953386"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VF954_00900"
     CDS             complement(<1..1014)
                     /locus_tag="VF954_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529663.1"
                     /translation="MTRRVTAHRPIGRYTCRGLAVAFLAGAGVYLLGAGAARADGAPD
                     KTGWWSEVQQAPVAIPLSTATGSNLVVGNDPTGADNVAAVQYTVPSGTSRASAGGHLS
                     LTIAPNTSVGTVQLAACPIAGDWQPVEGGPWTAKPNWSCAASAPGTVSSDGTTVSFAL
                     TAAVQSSAGVFSLAIVPATGAGAFQVQLSNPDASSFTATGGPTEEPPPDTSSNPLDTA
                     GPASASQAVLPAPEPSGSLPVDTVPPSPPVETSPAPGPASPSAPAASPALVQPAASSH
                     GAFNDRPHRVLAGAILALMAASAWWLGGSAARPPQLLGSLGARSAPAAGAAAAPPTGG
                     IGRF"
     gene            complement(1011..2714)
                     /locus_tag="VF954_00905"
     CDS             complement(1011..2714)
                     /locus_tag="VF954_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9529664.1"
                     /translation="MSAVAGGGPPILTCRGVDVAYDKVQVLFGVDLEVADGEIVALLG
                     TNGAGKSTLLKAISGLVTPAAGQIVFGGVDITRASPVVAAKLGIVQVPGGKAVFPTLT
                     VAEHFKAGAWLYARQGPAEVQDRIDEVLELFPRVRERWDQMAGNLSGGEQQQLALGMA
                     FVARPRLLVIDELSLGLAPTIVEQLLGIVRRIHGTGCTIILVEQSVNVALTISSRAYF
                     MEKGEVRFSGPTAELLERGDILRSVFLEGAGATRAGAKSGAAGKTTGKANGKKPPAGA
                     GPEQPAIMEVRGLTKRFGGICAVDDVSFDLRDNEILGLIGPNGAGKTTIFDLLSGILS
                     MDTGRIRLLGRDITRWRPDRRAVAGLGRSFQDARIFPSLTVAENVAIGLERHIEARDH
                     LAALLNLPEVQESEADVAYTVDDLIELMNLGAFRDKFVGELSTGTRRIVDLAMAIAHD
                     PSVLLLDEPSSGIAQRETEALGPLLRRIQAETGCAMLVIEHDMPLITSVSDEIIALEL
                     GAVVVRDTPDKVVTNPQVVSSYLGGDIDAINRSGRSSPKPSGPRTGRASGRAPRQRVA
                     R"
     gene            complement(2711..>3538)
                     /locus_tag="VF954_00910"
     CDS             complement(2711..>3538)
                     /locus_tag="VF954_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9529665.1"
                     /translation="VRIQGLYLAVTTLAFGYAMQNYFLNKNYWLAKRMLPGGFVASIR
                     RPLLWQRIDLENDRSFYFACLVLLALAMAAAQAFRRNRSGRILIAARDNQRAAPAYSV
                     NLVRTRLAAFAVSGGIAGMAGVLMAYDQHNVITGTYSVPASIFIFLAAVVGGLTSVPW
                     AVAGAVVFEYVVLFGPHFYDRWFGPHIVAIVPLLLTGPLLVLQLYLHPGGFAEAGFQA
                     RDRFLRWVARRHDILVPSLVADKRVEEAEDASSSEGASPSEVGGGQPQMATASKAER"
BASE COUNT          508 a         1235 c         1332 g          463 t
ORIGIN      
        1 gaacctgccg atcccacccg tcggcggggc ggccgccgcg ccggcagccg gcgccgatcg
       61 ggcgccgagc gaccccagca gctggggggg gcgagcggcg ctgccgccca gccaccacgc
      121 gctcgccgcc atcagggcca ggatggcgcc ggccaggacc cgatggggcc ggtcgttgaa
      181 ggcgccgtgg ctggacgcgg ccggctgcac cagcgcgggc gaggccgcgg gggccgacgg
      241 cgaagccggg cctggtgccg gggacgtctc gaccggcggc gagggtggca cggtatcgac
      301 cggcagggaa cccgacggct ccggcgcggg gagcacggcc tgcgacgcgg acgccggccc
      361 ggccgtgtcc agcgggttgg acgaggtgtc gggcggcggc tcctccgtgg ggccgccggt
      421 ggcggtgaag ctggacgcat ccggattgga gagctgcacc tggaaggctc ccgctcccgt
      481 agccggcacg atggcgaggg agaacacgcc cgccgagctc tgcaccgccg cggtgagggc
      541 gaaggacacc gtggtcccgt cgctcgacac cgtgccgggc gccgacgcgg cgcaggacca
      601 gttcggcttg gcggtccagg gcccgccttc gacgggctgc cagtcgccag cgatggggca
      661 cgcggccagc tgcacggtgc ccacgctggt gttgggcgcg atcgtcaagc tgaggtggcc
      721 accggcggac gcacgggagg tgccggacgg cacggtgtac tgcacggcgg cgacgttgtc
      781 cgcgccggtg gggtcgttgc ccaccaccag gttggacccg gtcgcggtcg acagggggat
      841 ggcgacgggc gcctgctgga cctcgctcca ccaacccgtc ttgtccggcg ccccgtcggc
      901 ccgggcggcc ccggcaccga gcaggtacac gccggcgccg gcgaggaacg cgacggccag
      961 gccccggcag gtgtaccggc cgatcggcct gtgggccgtc accctccggg tcacctggcc
     1021 acccgctgcc gcggggcccg gcccgaagcg cggccggtcc tcgggccgga cggcttcggt
     1081 gaggaccgtc ccgaccggtt gatggcgtcg atgtccccac cgaggtagga gctcaccacc
     1141 tgcgggttgg tcacgacctt gtcgggcgtg tcgcggacga cgaccgcgcc gagctcgagg
     1201 gcgatgatct cgtcggacac ggacgtgatc agcggcatgt cgtgctcgat caccagcatg
     1261 gcgcagccgg tctcggcctg gatacgcctg agcaggggcc cgagcgcctc cgtctcccgc
     1321 tgggcgattc cggacgaggg ctcgtcgagg agcagcaccg acgggtcgtg ggcgatcgcc
     1381 atggccaggt cgacgatccg gcgggtcccg gtcgacagct cgcccacgaa cttgtcccgg
     1441 aacgcaccca ggttcatgag ctcgatcagg tcgtccaccg tgtaggcgac gtcggcctcg
     1501 gactcctgga cctcgggcag gttcaacagg gccgccaggt ggtcgcgggc ctcgatgtgg
     1561 cgctcgaggc cgatggcgac gttctcggcc acggtgagcg aggggaagat ccgggcgtcc
     1621 tggaacgagc gccccagccc ggccaccgcc cggcggtctg ggcgccagcg ggttatatcc
     1681 cggcccagca gccggatgcg gcccgtgtcc atcgacagga tccccgagag caggtcgaag
     1741 atggtcgtct tgccggcgcc gttgggaccg atcagaccca ggatctcgtt gtctcgcagg
     1801 tcgaagctga cgtcgtcgac ggcgcagatg cccccgaagc gcttggtgag gcccctgacc
     1861 tccatgatcg ccggctgctc ggggccggcg ccggccggcg gcttcttgcc attggccttg
     1921 ccagtggtct tgccagcggc cccgctcttg gccccggccc gggtggcgcc ggcgccctcg
     1981 agaaagaccg accgcaggat gtcgccccgc tcgagcagct ccgcggtcgg cccggagaag
     2041 cggacctcgc ccttctccat gaagtaggcc cggctggaga tggtgagggc gacattgacg
     2101 gattgctcga cgaggatgat ggtgcagccc gtgccgtgga tccgccgcac gatgccgagg
     2161 agctgctcga cgatcgtggg ggccagcccg agggacagct cgtcgatgac gagcaggcgg
     2221 ggccgggcca cgaacgccat cccgagggcg agctgctgct gctcgccccc ggacaggttg
     2281 ccggccatct ggtcccagcg ctcccgcacc cgcgggaaca gctcgaggac ctcgtcgatg
     2341 cggtcctgca cctcggccgg gccctggcgg gcgtagagcc aggcgccggc cttgaagtgc
     2401 tcggccacgg tcagggtggg gaacacggcc ttgccgccgg gcacctgcac gatgccgagc
     2461 ttggccgcca ccaccgggct cgcccgggtg atgtccaccc cgccgaagac gatctggccc
     2521 gccgccgggg tcaccaggcc ggagatggcc ttgagcagcg tcgacttgcc cgccccgttg
     2581 gtgcccagca gggcgacgat ctcgccgtcg gcgacctcca ggtcgacccc gaagaggacc
     2641 tgcaccttgt cgtaggccac gtccacgccc cggcaggtga ggatcggggg cccgccgccc
     2701 gcgacggcgc tcatcgctcg gccttcgagg cggttgccat ctgcggctgg ccgccgccga
     2761 cctcgctcgg ggaggcgccc tcgctcgagg aggcgtcctc ggcctcttcc acccgcttgt
     2821 cggcgacgag cgacggcacg aggatgtcgt ggcggcgcgc cacccagcgc aggaaccggt
     2881 ccctcgcctg gaagccggcc tcggcgaagc caccgggatg gagatagagc tgaaggacca
     2941 gcagcgggcc ggtgagcagg aggggcacga tggccacgat gtggggcccg aaccagcggt
     3001 cgtagaaatg ggggccgaac aggaccacgt actcgaagac caccgcgccc gccaccgccc
     3061 acgggaccga cgtcagcccc ccgacgacgg cggcgaggaa gatgaagatg ctcgccggca
     3121 cgctgtaggt gcccgtgatc acgttgtgct ggtcgtaggc catgaggacg cccgccatgc
     3181 cggcgatccc gccggacacg gcgaaggccg ccaggcgggt tcgcacgagg ttgacggagt
     3241 aggcgggcgc ggcccgctgg ttgtcgcgcg ccgctatgag gatgcgcccg cttcggttgc
     3301 gccggaacgc ctgggccgcg gccatggcca gggccaggag gaccaggcag gcgaagtaga
     3361 agctgcggtc gttctccagg tcgatccgct gccacagcag aggccggcga atgctggcga
     3421 cgaatcctcc cggcaacatg cgcttggcca gccagtagtt cttgttgagg aagtagttct
     3481 gcatggcgta cccgaacgcc agcgtggtga cggccaggta caggccctgg atgcgcac
//
LOCUS       DASWGG010000054         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2953465, whole genome shotgun sequence.
ACCESSION   DASWGG010000054 DASWGG010000000
VERSION     DASWGG010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2953465"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VF954_00915"
     CDS             complement(<1..520)
                     /locus_tag="VF954_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX9529666.1"
                     /translation="MSTSATGEEIRRAYLRQARLLHPDVGGDDWPVQVLNEAWAVLGD
                     PERLRAYDRTLVGRRPPPPGPTSESGPRAGEDVYFGEEPEPTVPTEVRREVLLLVPLA
                     VLGLAVACFRLSVVMSSVALQITAVLLVPVAALCFVMVPILTLRQSRRGGPTGVAVRE
                     RAGSGRASTAPPT"
     gene            complement(737..1102)
                     /locus_tag="VF954_00920"
     CDS             complement(737..1102)
                     /locus_tag="VF954_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9529667.1"
                     /translation="MTPTSLPAVVTEYLGLAGSPRSADADAVLACFTDDAEVDDFEDV
                     RIGAEAIRRWWQGPATAYDYTVEVLGGHALGADRFVAFPRLFGDFPGGTAELADRFTL
                     RDGLIAQLEITATDPIGDR"
     gene            complement(1099..>1539)
                     /locus_tag="VF954_00925"
     CDS             complement(1099..>1539)
                     /locus_tag="VF954_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9529668.1"
                     /translation="PGAALDSPPAGGLMVTDTDSYQRPEEPLMMTTPTTIDPAQLPDV
                     ITGYLAAHVARDLDRAVASYQPDATVTDEGRTYYGHDEIRAWLTRSASEFTYTIEMTG
                     AAKLDDDHYDVTHHLEGNFPGGQVDLHFRFALRDDAIARLAIEP"
BASE COUNT          231 a          515 c          577 g          216 t
ORIGIN      
        1 tggtgggcgg ggcggtcgac gcccgcccac tccctgcgcg ctccctgacg gctacgccgg
       61 taggcccgcc gcgcctgctc tggcgaaggg tgaggatcgg gaccatcacg aagcacaggg
      121 cggcgacggg cacgaggagc accgcggtga tctgcagggc cactgacgac atcaccacgc
      181 tcaagcggaa gcaggccacg gccaggccga gcacggccaa gggcacgagc agcaggacct
      241 ccctccgcac ctcggtgggc accgtcggct cgggctcctc gccgaagtac acgtcctcgc
      301 cggcacgagg cccggactcg gaggtcgggc ccggcggggg cgggcggcgg cccacgagcg
      361 tgcggtcgta ggcccgcagt cgctcggggt cgcccagcac cgcccacgcc tcgttcagca
      421 cctgcacggg ccagtcgtca ccgcccacgt cagggtggag gaggcgagcc tgacgcaggt
      481 aggcgcgcct gatctcctcg ccggtggccg aggtggacac gcccagcatc tcgtgtgcgt
      541 cacccggcca aggctacgat ccgacgcgca tgcaggtcgt ggggggaagg tgtccgacca
      601 cgcgcccgtg cagggctcgc tgcgggggcg gcggggtcga tgtcggcgaa ggtgcgcacg
      661 aaccccggcg ggatcgctcg cacccgctcg gcgacgcgcg ccgcacagct ggtggcatcc
      721 ggatcagcgg agggcatcag cgatcaccga tcgggtcggt ggcggtgatc tcaagctggg
      781 cgatcagccc gtcgcgcagg gtgaaccggt cggccagctc cgccgtaccg ccgggaaagt
      841 caccaaaaag gcgggggaag gccacgaacc ggtcggcgcc gagagcgtgc ccgccgagca
      901 cctcgacggt gtagtcgtag gccgtggcgg gtccctgcca ccaccggcgg atcgcttcgg
      961 ctccgatacg gacgtcttcg aagtcgtcca cctcggcgtc atcggtgaag caggccagca
     1021 cagcatcggc gtcagccgat ctgggcgagc cggcgagccc caggtactcg gtgaccacgg
     1081 cgggcagcga cgtgggggtc acggctcgat cgccagtcgt gcgatggcgt cgtcgcgcag
     1141 ggcgaaccgg aagtgaaggt cgacttgccc acccggaaag ttcccctcca ggtggtgggt
     1201 gacgtcgtag tggtcatcgt cgagcttggc ggcgccggtc atctcgatcg tgtaggtgaa
     1261 ctcgctcgcc gagcgggtga gccacgctcg gatctcgtcg tggccgtaat aggtccggcc
     1321 ctcgtcggtg accgtggcgt cgggctggta gctggcgacc gcccggtcga ggtcgcgggc
     1381 cacatgggcg gccaggtagc cagtgatgac gtccggtagc tgggccgggt cgatcgtcgt
     1441 cggtgtggtc atcatcagtg gctcctccgg tcgctggtaa ctgtccgtgt cggtcaccat
     1501 cagccctccc gctgggggag agtcaagggc agctccggg
//
LOCUS       DASWGG010000055         4181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1341617, whole genome shotgun sequence.
ACCESSION   DASWGG010000055 DASWGG010000000
VERSION     DASWGG010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341617"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VF954_00930"
     CDS             complement(<1..1011)
                     /locus_tag="VF954_00930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9529669.1"
                     /translation="MDVTDVLGAPAPLRDVDPSPVSGPAPTRSVWLGAVVAVVLAVAV
                     ALRFWTRSDLWLDEAQTVAIARQPLHSLTGALRHDGAPPLHYVLLHAWIRVFGTSDLA
                     VRSLSGVLGVACLPLAWLAGRRAGRHRGEAAATATAWAALLLVATSPFAVHYSTEARM
                     YSLVVLLTLVGYLALTSILEDPAPRAGPVVALAVTTGLLLLTHYWAGFLLAALGAYLA
                     VLSVRQPEERRPRHALAAMAAGCLLFVPWLPAFAHQLRHTGTPWADPGSFTALVNAIS
                     EFAGGKTSAGRGLGLVFFALLGFGLFGRGVDERHVDIDLRTQPDSRGLGVVLVGALTI
                     AIA"
     gene            complement(1033..2844)
                     /locus_tag="VF954_00935"
     CDS             complement(1033..2844)
                     /locus_tag="VF954_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9529670.1"
                     /translation="MQLESLGQGRVFDGTQFDVAQSREWARRKRLGRLTIVIAGLCLW
                     MWSRLLSNRPIYPVPPHISGKLAQVAPLLLLVVILGAAVVLPLLTAGRSPHVLFRSSE
                     IDVSLDDVKGADVIVEEVVKSLNLFLAHKTFKEQMGGTPRRAILFEGPPGTGKTYMAK
                     AMAREAGVPFLFVSSSAFQSMYYGQTNRKIRSYFKALRAAARHEGGAIGFIEEIDAIG
                     ASRGGMGGSRGEGISGVVNELLIQLQSFDTPPPSTRLRRWGIERLNSLLPRHLQLAKP
                     GLTPANILVIGATNRAADLDPALLRPGRFDRSIYFDLPSRSGRREILDYYLAKKAHEP
                     DLDEPARRDTLAAMTSGYSPVMIEHLLDEALVWALRRGAGRLSWADLQRAKMTSEIGL
                     AQPVEYTQAERRTIATHESGHATVAWLVGKSRKLEVLSIIKRKDALGLLSHSDREERF
                     LKSQSEMHGLIQIALGGLAAEEVFFGEISSGPSGDLKVATTWAAMMVGSLGMAGSLFS
                     YEAVEGPPVNVVAKVSSTDEGKAKIEAILDAARAEARGMLEEHRGVVEGLRDALLERD
                     ELIGDEITDAIVAAATSGPAAPLAELQALDGSTPPDQ"
     gene            2963..>4181
                     /locus_tag="VF954_00940"
     CDS             2963..>4181
                     /locus_tag="VF954_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX9529671.1"
                     /translation="MIRRLCTVFLLPVLLPVVLGFSGLGLGLSAAGAVAAPAPPAVAP
                     LPPLAGAGGSATPSSFWLATGAGEVWAFGKAPGLGSLAGVALARPIVGIAPTVDAAGY
                     WLVASDGGIFAFGDAAFLGSTGALRLRQPIVGMATTPTGKGYWMVARDGGIFAFGDAG
                     FFGSTGATTLNKPIVGMAPTRSGRGYWLVASDGGIFAFGDARFFGSTGAVHLNQPIVA
                     MAAPPAGTGYWLLASDGGVFTFGAAPFGGSMGAAPTLDAAQRLVPSGTGKGYWIVDQN
                     GTVHPFGDATGAPPVQGLMFGAVTAGDRATLFAFHQLGKPYIWGGNGPAGYDCSGLAL
                     ASWQTVGVSFARVANDQYHTAGAPVALSDLQTGDLVFWGTDQGDWTTVYHAAMFVGGG
                     RIAKGEQSAVGRDQ"
BASE COUNT          575 a         1451 c         1577 g          578 t
ORIGIN      
        1 agcgatggcg atggtgaggg ctccgaccag gaccacgccg agcccgcggc tgtcgggctg
       61 ggtgcgcagg tcgatgtcga cgtggcgctc gtcgacgccc cgcccgaaca ggccgaagcc
      121 gagcagggcg aagaacacca ggccgaggcc ccggcccgcg ctggtcttgc cgccggcgaa
      181 ctcgctgatg gcgttgacga gggccgtgaa cgagcccggg tcggcccacg gcgtgccggt
      241 gtggcgcagc tggtgggcga aggccggcag ccacggcacg aagagcaggc acccggcggc
      301 catggccgcg agggcgtggc ggggccggcg ctcctcgggc tggcggacgg agaggacggc
      361 gaggtaggcg ccgagggcgg ccagcaggaa cccggcccag tagtgggtca gcaggagcag
      421 gccggtcgtc accgccaggg cgaccaccgg gccggccctc ggcgcggggt cctcgaggat
      481 cgaggtcagg gccaggtacc cgaccagggt gagcagcacg accagggagt acatgcgcgc
      541 ctcggtgctg tagtgcaccg cgaacgggct ggtggcgacc agcagcaggg cggcccacgc
      601 cgtggcggtg gcggccgcct cgccccggtg gcgcccggcc cggcgcccgg ccagccaggc
      661 gagcgggagg caggccacgc cgagcacgcc cgacagcgac cgcacggcca ggtcgctggt
      721 cccgaacacc cggatccacg cgtgcagcag gacgtagtgc agcggcgggg ccccgtcgtg
      781 gcgcagggcg cctgtcagac tgtggagggg ttggcgggcg atggcgaccg tctgggcctc
      841 gtcgagccac agatcggacc gggtccagaa acgcagggcc acggcaacgg cgagcacgac
      901 ggcgacgacg gcgccgagcc agacggagcg ggtcggcgcc ggcccgctga ccggcgaagg
      961 gtcgacgtcc cggagagggg cgggcgcgcc cagcacgtcg gtgacgtcca tggcgcccgc
     1021 agcgtagcgg ggctactggt caggcggggt ggagccgtcc agcgcctgga gctcggccaa
     1081 cggcgcggcc ggcccggagg tggcggccgc cacgatggcg tcggtgatct cgtctccgat
     1141 gagctcgtcg cgctccagca gtgcgtcccg gagaccctcg acgacgccgc ggtgctcttc
     1201 gagcatgccg cgggcctcgg ccctggcggc gtccaggatg gcctcgatct tggctttccc
     1261 ctcgtccgtg ctcgacacct tggcgaccac gttgaccggc ggcccctcga cggcctcgta
     1321 ggagaagagc gagccggcca tgcccaggct gccgaccatc atggcggccc aggtggtggc
     1381 caccttgaga tcgcccgacg ggcccgagct gatctccccg aagaagacct cttcggccgc
     1441 cagcccgccc agcgcgatct ggatgaggcc gtgcatctcg gactgcgact tcaagaagcg
     1501 ctcctccctg tcggagtggg agagcagccc gagggcgtcc ttgcgcttga tgattgacag
     1561 gacctcgagc ttgcggctct tgcccaccag ccaggccacg gtcgcgtgcc cgctctcgtg
     1621 ggtggcgatg gtccggcgct ccgcctgggt gtactcgacc ggctgggcca ggccgatctc
     1681 ggatgtcatc ttggcccgct ggaggtcggc ccaggacagc cggccggcgc cccggcgcag
     1741 ggcccacacc agcgcctcgt ccaggaggtg ctcgatcatg acgggcgaat accccgacgt
     1801 catggcggcc agcgtgtcgc gccgggcggg ctcgtcgagg tcgggctcgt gggccttctt
     1861 ggccaggtag tagtcgagga tctcgcgccg gcccgagcgg ctgggcaggt cgaagtagat
     1921 ggagcggtcg aaccggcccg gtcgcagcag ggcggggtcg aggtcggcgg cccggttggt
     1981 ggcgccgatg accaggatgt tggccggggt gagccccggc ttcgccagct ggaggtggcg
     2041 gggaagcagg ctgttgaggc gctcgatgcc ccagcgccgg aggcgggtgg agggcggcgg
     2101 cgtgtcgaag gactggagct ggatcagcag ctcgttgacc acgccggaga tgccctcgcc
     2161 ccgcgacccg cccatcccgc cgcgggacgc cccgatggcg tcgatctcct cgatgaagcc
     2221 gatggcgccg ccctcgtggc gggcggcggc gcgcagggcc ttgaagtacg accggatctt
     2281 gcggttggtc tggccgtagt acatggactg gaaggccgac gaggagacga acaggaacgg
     2341 cacgccggct tcccgggcca tggccttggc catgtaggtc ttgcccgtcc ccggcgggcc
     2401 ttcgaacaag atggcccgcc tcggcgtgcc gcccatctgc tccttgaacg tcttgtgggc
     2461 gaggaacagg ttcaggctct tgacgacctc ctccacgatc acgtccgccc ccttgacgtc
     2521 gtcgagcgag acgtcgatct cgctggaccg gaacaggacg tgcggcgagc gccccgcggt
     2581 caggaggggg agcacgaccg ctgcgccgag gatgaccacg agcagcagca gcggggcgac
     2641 ctgggccaat ttgcccgaga tgtgcggggg aaccgggtag atcgggcggt tggacagcag
     2701 ccgcgaccac atccacaggc agaggccggc aatgacgatc gtcagccgtc cgaggcgctt
     2761 gcgccgggcc cactcccggg actgcgccac gtcgaactgc gtgccgtcga acacccgacc
     2821 ctggcccagg ctctccaact gcatgatgcg ctcctcgtga tgcctcaaca tagctagttc
     2881 acttctcgcc ccatccgtca agtcctctac cgatccacac gacgcgcttg aggcgggctt
     2941 ggggcgatcc gatgcacatg gggtgattcg tcgtctctgc accgtctttc tgctgcccgt
     3001 cctcctgccc gtcgtgctcg ggttctccgg gctggggctg ggcctgtcgg ctgccggcgc
     3061 ggtggccgcg ccggcgcccc cggccgtggc gcctctgccc cccctcgcgg gggcgggtgg
     3121 cagcgccacg ccgagcagct tctggctggc gaccggagcg ggtgaggtgt gggccttcgg
     3181 gaaggcgccc ggcctcggct cgctggccgg ggtggcactg gcccggccga tcgtgggcat
     3241 cgccccgacg gtcgacgcgg ccggctactg gctggtggcg tcggacggcg ggatcttcgc
     3301 gttcggcgat gccgcgttcc tggggtcgac gggcgcgctg cgcctgcgcc agccgatcgt
     3361 cggcatggcg accacgccca ccggcaaggg ctactggatg gtggcccgcg acggagggat
     3421 cttcgccttc ggcgatgccg gcttcttcgg ctccactggg gccacgaccc tcaacaagcc
     3481 catcgtcggc atggccccga cccggtccgg ccggggctac tggctggtgg cgtcggacgg
     3541 agggatcttc gccttcggcg acgcccgctt cttcggctcc acgggcgccg tccacctcaa
     3601 ccagcccatc gtcgccatgg cggcgccccc ggccggcacg ggctactggc tgctcgcctc
     3661 cgacggcggc gtcttcacct tcggggcggc gcccttcggc gggtccatgg gcgcggcgcc
     3721 caccctcgat gccgcccagc ggctggtgcc gtcgggcaca ggcaagggct actggatcgt
     3781 ggaccagaac ggcaccgtcc accccttcgg cgacgcgacg ggcgcgccgc cggtgcaggg
     3841 gctgatgttc ggcgcggtca cggcgggcga ccgggcgacc ctgttcgcct tccaccagct
     3901 gggaaagccc tacatctggg gcgggaacgg cccggccggc tacgactgct cgggtttggc
     3961 cctggcgtcg tggcagaccg tcggcgtgag cttcgcccgg gtcgccaacg accagtacca
     4021 cacggccggg gcgccggtcg cgctcagcga cctccagacg ggcgacctgg tgttctgggg
     4081 gaccgaccag ggcgactgga cgacggtgta ccacgcggcc atgttcgtgg gcggagggcg
     4141 catcgccaaa ggcgaacaat ccgccgtcgg acgcgaccaa c
//
LOCUS       DASWGG010000056         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1668050, whole genome shotgun sequence.
ACCESSION   DASWGG010000056 DASWGG010000000
VERSION     DASWGG010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668050"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..>2157)
                     /locus_tag="VF954_00945"
     CDS             complement(315..>2157)
                     /locus_tag="VF954_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529672.1"
                     /translation="TVRPRHVIIATMPGVMWEDVAAGRAPALRALGERWSMAAVSVRT
                     APAHTDADSAMTTIGAGNRARGRTLADNAGLHFGAVPGALGATLHEHGLRTAASDATV
                     APAVADPSHPMDATDPLAADVGAVDLTGGDAALADLVGRLDLAQDTLFVLSPYQPAGR
                     PPRLMAAVMAGVGSTSGGWMTSATTHRDGLVTLPDVAPGILGLLGVKAPASMTGQALR
                     SVPAGPAGANRLQELLGLQAAAVSYGRRLVPFNVALATIGTLVFLAGWRRLRLGATGP
                     PRGAALLRATGLLVAAAPVACLAQAAVGGERWPALPAFAFLVAAALAVVAIAVGGPWR
                     RSHSGPPVFVATVTALAIAGDLATGSRGQITSLIGYSPLVAGRFYGLSPVSFATLATS
                     ALLLAGLAAGARPKQSVAIVALIGLLTVGLAGAPVFGAKFGSILTLVPAFGLLALLVS
                     GRRLSLAKVGLLAAAAAAVALGIGVVDALRPASSQTHIGRFVAALLGGGAGTVGDVIH
                     RKLDANLGIYLRAPVALLVPVALGFVGVAILRPARVVEGPLRAVPGLRFGLLAAVAAM
                     ALGLVLNDSGAAIPAMGMAVGVPWAIALLVGVAAAKPARTPVAGPPG"
BASE COUNT          323 a          789 c          829 g          216 t
ORIGIN      
        1 ttgccgcggc cttgacgacc aacgcctggg gctcatacgg gctgggctcg gggaccacgg
       61 ccccgctgcc gtcgccccag ccgtcagcct cgcccactgg cgcgaaataa cagcacgttt
      121 gcggctctac caccacgggg atcttctttg ctccaaaaag tgcggatagc gcaccttttg
      181 gagcaaagta cgccgtccgt cagagccgcc gacgaggcca agggctggtg cggaggcggt
      241 gcgggtggcc gtgccgccgg tgccgccagg gccgatctcg atctggtggc cggtcgggac
      301 gacgagcctc atcctcagcc tgggggccct gcgactggcg tacgggccgg cttggccgcc
      361 gcgactccga cgagcagggc gatagcccag gggaccccga ccgccatccc catcgcaggg
      421 atcgcggccc ccgagtcgtt gaggacgagg cccagcgcca tggcggccac ggccgccagc
      481 aggccgaacc gcaggcccgg cacggctcgc aacgggcctt cgaccacgcg ggcggggcgc
      541 aggatggcca ccccgacgaa gcccagggcg accgggacga gcagagcgac gggggccctc
      601 aggtagatcc cgaggttggc gtcgagcttg cggtggatca cgtcgcccac cgtccccgcc
      661 ccgccgccga gcagcgccgc cacgaaccgg ccgatgtggg tctgggaact ggcgggccgc
      721 agggcgtcca cgacgccgat gcccagggcg acggccgccg ccgcggcggc cagcaggccg
      781 accttggcga gggagagccg tcgccccgac acgagcagtg ccagcagccc gaaggccggc
      841 accagggtga ggatgctgcc gaacttggcg ccgaagaccg gggccccggc gaggccgacg
      901 gtgaggaggc cgataagcgc aacgatcgcc acgctctgct tcgggcgggc acccgcggcc
      961 aaaccggcga gcagcagggc agaggtggcg agcgtcgcga aggacacggg cgacagcccg
     1021 tagaagcgtc ccgccacgag cggcgagtag ccgatcaggc tggtgatctg cccccgcgac
     1081 ccggtcgcga ggtccccggc gatggcgagg gccgtcaccg tcgcgacgaa caccggcggc
     1141 cccgaatggc tgcgccgcca cggtcccccg acggcgatgg ccacgacggc gagggccgcc
     1201 gccacgagga acgcgaacgc cggcagggcc ggccagcgct ccccgccgac cgcagcctgg
     1261 gccaggcagg cgaccggagc ggcagccacg aggaggccgg tcgcccgcag caacgccgcg
     1321 cccctgggcg gcccggtggc cccgaggcgg agcctgcgcc agccggccag gaagaccagg
     1381 gtgccgatgg tagcgagcgc gacgttgaac ggcaccagcc gccgtccgta ggagacggcg
     1441 gcggcctgca gccccagcag ctcctgcagc cggttggccc cggccggccc cgccgggacc
     1501 gagcggaggg cctggccggt catggaggcg ggggccttga cgccgaggag cccgagaatg
     1561 ccgggggcga cgtccgggag cgtgacgagc ccgtcgcggt gcgtcgtcgc cgaggtcatc
     1621 cacccgcccg aggtcgaccc gacgccggcc atgacggccg ccatcaggcg cgggggccgc
     1681 ccggccggct ggtagggcga cagcacgaac agggtgtcct gcgccaggtc gaggcgtccg
     1741 acgagatcgg ccaacgcggc gtcgccgccg gtcagatcga cggcgccgac atcggctgcc
     1801 aacgggtccg tggcgtccat cgggtgcgag ggatccgcca ccgccggggc cactgtggcg
     1861 tcggaggcgg cggtgcgcag gccgtgctcg tgcagggtcg caccgagggc tccgggcacc
     1921 gcaccgaaat gcagccctgc gttgtcggcc agggtccggc cgcgggcccg gttgccggcg
     1981 ccgatggtgg tcatggccga atcggcgtcg gtgtgcgcgg gcgccgtccg gacggacacg
     2041 gccgccatcg accagcgctc cccgagcgcc cgcagcgcgg gggcccggcc ggccgcgacg
     2101 tcctcccaca tgaccccggg catcgtggca atgatcacgt ggcgcggccg tacggtg
//
LOCUS       DASWGG010000057         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1023412, whole genome shotgun sequence.
ACCESSION   DASWGG010000057 DASWGG010000000
VERSION     DASWGG010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023412"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VF954_00950"
     CDS             <1..248
                     /locus_tag="VF954_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX9529673.1"
                     /translation="AGWVPYSKQVGQTGKVVKPKLYIALGISGATQHMVGMKGSKNII
                     AINKDQEAPIFSIADLGIVGDVHKVVPKLIEAIKARQ"
     gene            complement(253..972)
                     /locus_tag="VF954_00955"
     CDS             complement(253..972)
                     /locus_tag="VF954_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEX9529674.1"
                     /translation="MSPDQRQPAADALRVLAEGDLEVEGRMPWSSNATFLARACLGGE
                     QMAVIYKPHRGERALWDFPGGLYRREAAAYELSVALGWPIVPETLVRHDAPLGTGSLQ
                     RFVDADFSEHYFTLVENPAHHDALRRMAVFDLLLNNADRKSGHCLLDGDHIWGIDHGV
                     CFHVDPKLRTVIWDFGGEEVPPGLLADVRRLSAAVPAPLLGLLEEDEVAALMRRAEGM
                     VRMGRFPDPDPDRRPYPWPLV"
     gene            complement(933..1430)
                     /locus_tag="VF954_00960"
     CDS             complement(933..1430)
                     /locus_tag="VF954_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HEX9529675.1"
                     /translation="MSESFDFPAPERITVGAVGPPGQRVFLLQASDGSDHVTLKVEKA
                     QVSALATHLGRVLTELTRPAHMPEDLELAEPAEPAWVVGSLGIGYDEDNDRILLLAEE
                     AVGEDAEGAVARFALTREAAAALAVRGTRLVEAGRPPCPLCGYPLDPSGHACPRTNGN
                     RPPTL"
     gene            complement(1427..2074)
                     /locus_tag="VF954_00965"
     CDS             complement(1427..2074)
                     /locus_tag="VF954_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HEX9529676.1"
                     /translation="MPTRRAKPPPGTLVLFVRHGRTPTTGDLLPGRAPGLHLADDGRA
                     QADAVAERLSAVTRIAAVYASPLERTRETAETIGRRLGLKVVAEKGLLECDFGDWTGA
                     KLKDLMKLPEWGTVQRWPSGFRFPNGESFSEMQARTTGAVDRLRERHRGESIVLVSHA
                     DPIKAAVAQALGSHLDLFQRIVVSPCSVTAVAYGGGGPTVLAVNSLGSLTDLKPA"
     gene            2147..>2394
                     /locus_tag="VF954_00970"
     CDS             2147..>2394
                     /locus_tag="VF954_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX9529677.1"
                     /translation="MQDEIVSCRACPRLVAWREEVAAVKRAAFQDQRYWGRPVPGFGD
                     AAARLLVVGLAPAAHGGNRTGRVFTGDRSGDWLFAALWR"
BASE COUNT          341 a          840 c          881 g          332 t
ORIGIN      
        1 acgccggctg ggtgccgtac tccaagcagg tgggccagac cggcaaggtc gtgaagccga
       61 agctctacat cgcgctcggc atctccggcg ccacccagca catggtcggc atgaagggct
      121 ccaagaacat catcgccatc aacaaggacc aggaggcgcc gatcttctcc atcgccgacc
      181 tcggcatcgt gggcgacgtg cacaaggtcg tgccgaagct catcgaggcc atcaaggcac
      241 gccaatagat agctagacga gcggccaggg gtagggccgg cggtcggggt cggggtcggg
      301 gaaacggccc atccggacca tgccctcggc gcgccgcatc agcgcggcca cctcgtcttc
      361 ctccagcagc ccgaggaggg gtgcgggcac ggcggccgag agccgccgca cgtcggcgag
      421 caggccgggc ggcacctcct ccccgccgaa gtcccagatg acggtacgca gcttggggtc
      481 gacgtggaag cacacgccgt gatcgatgcc ccagatgtgg tccccgtcga gcaggcagtg
      541 cccgctcttg cggtcggcgt tgttgagcag caggtcgaac accgccatcc gccggagggc
      601 gtcgtggtgg gccgggttct cgaccagggt gaagtagtgc tcggagaagt cggcgtccac
      661 gaagcgctgg agcgagcccg tccccaacgg ggcgtcgtgg cgcacgagcg tctcgggcac
      721 gatcggccac cccaacgcca ccgacagctc gtatgcggcc gcttcccggc ggtagaggcc
      781 accggggaag tcccagagag cgcgctcgcc gcggtggggc ttgtagatca cggccatctg
      841 ctcgccgccc aggcaggcgc gcgccaggaa cgtggcgttg ctgctccacg gcatccggcc
      901 ctcgacctcg aggtcgccct cggccaggac cctcagagcg tcggcggccg gttgccgttg
      961 gtccggggac acgcgtggcc ggagggatcg agcgggtagc cgcagagcgg gcagggcggg
     1021 cgcccggcct cgaccaggcg ggtgccccgg acggccaggg cggcggcggc ctcacgggtc
     1081 agggcgaaac gggcgacagc tccctcggcg tcctcgccga cggcctcctc ggcgagcagg
     1141 aggatgcggt cgttgtcctc gtcgtagccg attccgaggc tgcccacgac ccaggccggc
     1201 tcggcgggct cggccagctc caggtcctcc ggcatgtgag ccggtcgagt gagctccgtc
     1261 aggacgcggc cgaggtgggt ggctagcgcg gacacctggg ccttctcgac cttgagcgta
     1321 acgtggtccg agccgtcgct ggcctgaagg aggaagaccc gctggccggg gggcccgacg
     1381 gcgcccacgg tgatgcgctc cggggccggg aagtcgaacg actcgctcat gccggcttca
     1441 ggtcggtgag ggaccccagc gagttcacgg cgaggacggt cggccctccc cctccatagg
     1501 cgacggcggt caccgagcac ggcgacacca cgatgcgctg gaagagatcg aggtggctgc
     1561 cgagcgcctg ggccacggcg gctttgatgg gatcggcgtg cgacaccagg acgatcgact
     1621 cgccccggtg ccgttcccgg aggcggtcga ccgcgcccgt cgtgcgcgcc tgcatctcgg
     1681 agaacgattc gccgttgggg aaccggaagc cgctcggcca gcgctgcacg gtgccccact
     1741 ccggcagctt catcaggtcc ttgagcttgg cccccgtcca gtcgccgaag tcgcattcca
     1801 gcagcccctt ctcggccacg accttcagcc cgagccgccg gccgatggtc tccgcggtct
     1861 cgcgcgtgcg ttccaggggg ctggcgtaga cggccgcgat cctggtcacc gcagagaggc
     1921 gctcggcaac tgcgtcggcc tgagcccggc cgtcgtcggc cagatgcagg cccggcgcgc
     1981 ggccgggcag caggtcgccg gtcgtcggcg tcctgccatg acggacgaag agcacgagcg
     2041 tgccgggcgg tggcttggcg cgtcgggtcg gcatgccaaa tccaacctac tcggggcccc
     2101 acctagtctg gccccggtgg agcccgccgc cgccgccctc tgtcgtttgc aggacgagat
     2161 cgtgtcgtgc cgggcctgcc cccgcctcgt ggcgtggcgg gaggaggtcg ctgccgtcaa
     2221 gcgggccgcc ttccaggacc agcggtattg gggccggccc gtgcccggat tcggcgacgc
     2281 cgccgcccgc ctgctcgtcg tggggttggc gcccgccgcg catggtggga accgcaccgg
     2341 ccgggtcttc accggcgacc ggtcgggcga ctggctcttc gccgccctgt ggcg
//
LOCUS       DASWGG010000058         3916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1343952, whole genome shotgun sequence.
ACCESSION   DASWGG010000058 DASWGG010000000
VERSION     DASWGG010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343952"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VF954_00975"
     CDS             <1..345
                     /locus_tag="VF954_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX9529678.1"
                     /translation="CWQAVLEAWQAGAVVAGSSAGAMVITDPMVDPRGGAFTLGLGLI
                     EQLAVVPHYDHWSPDKAHRTVSLAAAGLPVVGIDEQTALIRDPAGAWRVEGAGGVTVY
                     QDGALAGMEALP"
     gene            complement(361..921)
                     /locus_tag="VF954_00980"
     CDS             complement(361..921)
                     /locus_tag="VF954_00980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9529679.1"
                     /translation="MPPTECPAPDGSSAKRQRFDGPPPICIDPVKRYTAEMVTSKGTM
                     AIALDPVAAPKTVNNFVVLARYHYFEGISFHRVIPGFVLQGGDPEGSGRGGPGYKFED
                     ELPRPGRYDLGSLAMANAGPNTNGSQFFVISGPDGMRLPPQYSLFGKVVKGLDVVAAI
                     DAVGTKGAGTPTERVVIESVTVTEEG"
     gene            complement(954..1754)
                     /locus_tag="VF954_00985"
     CDS             complement(954..1754)
                     /locus_tag="VF954_00985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9529680.1"
                     /translation="MPSQKRQRQRAGQQARRQTLLAQQKRARQRKMFIRYGAIAAVVA
                     IILAVLALNSGGKSKKTNVTSSSTSSTSTTLAAASYGTTACPPTDGSAPRTTTFGDSF
                     KKCIDPAKTYTATMQFDAGTVTIALDAKSSPITVNSFVALSRYHFYDGLTCHRVIKKF
                     VDQCGDPKGDGSGGPGFTIGEEPPKSGKYSIGDLAMAKTSAAHSTGSQFFLIVGDQGA
                     ALPAQYSLLGTITSGVDVAHAIEADGADQDPSPPKVVHKIVKVTISET"
     gene            1800..3029
                     /locus_tag="VF954_00990"
     CDS             1800..3029
                     /locus_tag="VF954_00990"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX9529681.1"
                     /translation="MPLVVQKFGGTSVADPDRILEVADHVVRTRRHGSDVVLVVSAMG
                     KETDDLLHLAGQVAKVPPGREMDMLITAGERKAMALVTMAITELGVPAASFTGSQAGF
                     ITDTTHGNAKILEMRPERLRESLTRGEVPVVGGSQGMSTTREVTFLGRGGSDTTAVAL
                     AAALGADACELYTDVTGVFTADPRVVPGARRMAVVSFDEMLEMTATGCPKPAMRSVEF
                     ARRHHVPLHVRSAFTWETGTWVREEDPDMEQPIISAVTHDTSEAKVTVTGVPDQPGIA
                     ARLFRSLADEHVNVDMIVQNVSTGGHTDISFTVPRSDLAVGLSVVHAHAAEIDATSVT
                     HDGEIGRVSLIGAGMKSNPGVAATMFEVLADHGINIEMISTSAIRISCVVREDAVEKA
                     VQVLHDAFGLDEPGGGF"
     gene            3083..>3916
                     /locus_tag="VF954_00995"
     CDS             3083..>3916
                     /locus_tag="VF954_00995"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX9529682.1"
                     /translation="MRVGVFGATGQVGGVMRTLLAERGVPVDQVRFFASARSAGRRLP
                     WGTAEIEVEDSANADFAGLDVALFSNGAAASREYAPRVAAAGAVVIDNSSAWRMDPGV
                     PLVVPEVNGHALDSIPLGIVANPNCTTMVAMPVLKPLDGEAGLRRLVISTYQAVSGAG
                     GSGVAELDKQVREAADRAAELTFDGTAVHFPPAEEFPATIAFNVVPQAGALLEDGSGE
                     TREERKLAEESRKILELPDLAVCATCVRVPVFTGHSLSINAGFARPLSPERARKILAT
                     AP"
BASE COUNT          541 a         1324 c         1441 g          610 t
ORIGIN      
        1 tgctggcagg cggtcctcga ggcgtggcag gccggggcgg tcgtggccgg ctcttcggcc
       61 ggcgccatgg tgatcaccga tccgatggtc gacccccggg gcggggcgtt cacgctgggt
      121 ctggggctga tcgagcaact ggccgtcgtt ccccactacg accactggtc gcccgacaag
      181 gcccaccgca cggtctcgct ggcggccgcc ggtctcccag tggtgggcat cgacgagcag
      241 acagcgctca tccgggatcc cgccggggcg tggcgggtgg aaggcgccgg cggggtgacc
      301 gtgtaccagg acggggcgct ggccgggatg gaggctctcc cctagcggcc gtagcggcgg
      361 ctacccctct tcggtgacgg tgaccgactc gatgacgacg cgctcggtcg gggtcccagc
      421 gcccttggtg ccgaccgcgt cgatggcggc caccacgtcc aggcccttga ccaccttgcc
      481 gaagagcgag tactggggcg gcagccgcat gccgtccggg ccgctgatca cgaagaactg
      541 gctgccgttg gtgttgggcc cggcgttggc catggccagc gagccgagat cgtagcggcc
      601 cggcctgggc agctcgtcct cgaacttgta gcccggcccg ccccgcccgc tgccctcggg
      661 atcgccgccc tggagcacga agccggggat gacccggtgg aaggagatcc cctcgaagta
      721 gtggtaccgg gccaggacca cgaagttgtt gaccgtcttc ggcgcggcga cgggatcgag
      781 ggcgatggcc atggttccct tcgaggtgac catctcggcc gtgtagcgct tgaccgggtc
      841 gatgcagatg ggcggggggc cgtcgaagcg ctggcgcttg gcgctcgacc cgtcgggcgc
      901 ggggcattcg gtggggggca tggggctcct tcccggccgg agccggtgga cggtcaggtc
      961 tcggagatgg tcaccttgac gatcttgtgc acgaccttcg gtgggctggg gtcctggtcg
     1021 gccccgtccg cctcgatggc gtgggccacg tcgacgccgg aggtgatggt tccgagcagc
     1081 gagtactggg ccgggagggc cgcaccctgg tcgccgacga tgaggaagaa ctggctccca
     1141 gtgctgtgcg ccgcgctcgt cttggccatg gcgaggtcgc cgatcgagta cttccctgac
     1201 ttgggcggct cctccccgat ggtgaagccc gggccacccg agccgtcgcc cttcgggtca
     1261 ccgcactggt cgacgaactt cttgatcacc cggtggcagg tcagcccgtc gtagaagtgg
     1321 tatcggctca gggccacgaa gctgttcacg gtgatcggcg agctcttggc gtcgagggcg
     1381 atggtcaccg tcccggcgtc gaactgcatg gtcgcggtgt aggtcttggc cgggtcgatg
     1441 cacttcttga acgagtcgcc gaacgtcgtc gttcgcggcg cgctcccatc cgtcgggggg
     1501 catgccgtgg tcccgtacga tgcggccgcg agcgtcgtcg aggtggacga ggtggacgag
     1561 gaggtgacgt tggtcttctt ggacttgccg ccgctgttga gggccagaac cgcgaggatg
     1621 atggccacga cggcggcgat ggcgccgtag cggatgaaca tcttgcgttg gcgggcgcgc
     1681 ttttgctggg cgaggagggt ctgacggcgg gcctgctgac ccgctcgctg gcgctgacgc
     1741 ttctggctgg gcacgggcgg caaacctagc cggtggggcc ggacgggtag cttcggctcg
     1801 tgcccctcgt cgtgcagaag ttcggcggaa cgtccgtcgc cgacccggat cgcatcctcg
     1861 aggtggccga ccacgttgtg cggacccgcc gccacggcag cgacgtggtg ctcgtggtct
     1921 cggccatggg caaggagacc gacgatctcc tccatctcgc cgggcaggtc gccaaggtgc
     1981 cgccgggccg ggagatggac atgctgatca cggccggcga gcgcaaggcg atggcgctcg
     2041 tcaccatggc catcaccgag ctcggggtgc cggccgcctc gttcacaggc agccaggccg
     2101 gcttcatcac cgacaccacc cacggcaacg ccaagatcct cgagatgcgg cccgagcgcc
     2161 tgcgcgagtc gctcacccgc ggcgaggtgc cggtggtcgg cgggtcccag ggcatgtcga
     2221 ccacgcgaga ggtgacgttc ctgggccggg gcggctcgga tacgaccgcg gtggccctgg
     2281 ccgccgccct gggggccgac gcgtgcgagc tgtacaccga cgtcacgggg gtcttcaccg
     2341 ccgacccgag ggtcgtcccc ggggcccggc gcatggcggt cgtgtccttc gatgagatgc
     2401 tggagatgac cgcgacgggc tgtccgaagc ccgccatgcg ctcggtggag ttcgcccggc
     2461 gccaccacgt gccgttgcac gtgcgctcgg ccttcacctg ggagaccggt acatgggtac
     2521 gcgaggagga cccggacatg gagcagccca tcatctcggc cgtgacccac gacacgagcg
     2581 aggccaaggt gacggtgacg ggcgtccccg accagcccgg catcgcggcc cggctcttcc
     2641 gcagcttggc cgacgagcac gtcaacgtcg acatgatcgt gcagaacgtg tcgaccggcg
     2701 gccacaccga catctccttc acggtgccca ggagcgacct ggcggtgggc ctctcggtcg
     2761 tccacgccca cgccgccgag atcgacgcca cctcggtcac ccacgacggc gagatcggcc
     2821 gggtcagcct catcggcgcg ggcatgaagt ccaacccggg cgtggcggcc accatgttcg
     2881 aggtgctggc cgaccacggg atcaacatag agatgatctc gacctcggcc atacgcatca
     2941 gctgcgtcgt gcgtgaggat gccgtggaga aagccgtgca ggtgctccac gacgccttcg
     3001 gcctggacga gcccggcggc ggcttctgat cgggcgtcca atggccgggg gccggtccag
     3061 gcgcctcgta caatcggagc ccatgcgagt cggtgtcttc ggagctaccg gtcaggtcgg
     3121 cggggtgatg cgcaccctgc tggccgagcg cggcgtcccg gtcgaccagg tccggttctt
     3181 cgcctcggcc cgcagcgccg gccggcggct gccatggggg acagccgaga tcgaggtcga
     3241 ggactcggcg aacgcggact tcgctggcct cgacgtcgcc cttttctcca acggggccgc
     3301 cgcttcgcgt gagtacgcgc cccgggtggc agccgcgggg gccgtcgtca tcgacaactc
     3361 gtcggcgtgg cggatggacc ccggcgtgcc actggtcgtg cccgaggtca acgggcacgc
     3421 cctcgactcc atcccgctcg ggatcgtggc caaccccaac tgcacgacca tggttgccat
     3481 gcccgtgctc aaacccctcg acggggaggc tgggctgcgc cggctggtga tctccaccta
     3541 ccaggccgtg tcgggcgcgg gcggatcggg ggtggccgag ctcgacaagc aggtccggga
     3601 ggccgccgac cgggccgccg agctgacctt cgacgggacc gccgtgcact tcccgccggc
     3661 cgaggagttc ccggccacca tcgccttcaa cgtggtgccc caggccgggg cgctgctcga
     3721 ggacggctcg ggcgagacca gggaggagcg caagctggcg gaggagtcca ggaagatcct
     3781 cgagctgccc gacctggcgg tgtgcgcgac ctgcgttcgg gtcccggtgt tcaccggcca
     3841 ctcgctgtcg atcaacgccg gcttcgcccg ccccctgagc ccggagcggg cccgcaagat
     3901 cctggccacc gcgccc
//
LOCUS       DASWGG010000059         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1669766, whole genome shotgun sequence.
ACCESSION   DASWGG010000059 DASWGG010000000
VERSION     DASWGG010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669766"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VF954_01000"
     CDS             <1..269
                     /locus_tag="VF954_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529683.1"
                     /translation="VGHAYEARARAGVDVGAMATDLLRPSAAVALGGGAVLLARHALS
                     LGADTSVGPLLVLGGIFTAVYLAVWLLGASAVPDDLRTLRSRLI"
     gene            complement(262..1005)
                     /locus_tag="VF954_01005"
     CDS             complement(262..1005)
                     /locus_tag="VF954_01005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9529684.1"
                     /translation="MVRSVWALGARRRGAGPVLAGVATRAPGRAASGDGGRGTVGRAY
                     GRVLRIPRRPAGSGAQHRGAGRAAARARRDPVPAGCRAIAGRRRSLERLVQLHASQWG
                     SRSGFSSAAPAFVAAATAGMGRGEVSVHELAADDEVGASLVTFELSGRWSYYQAGRRL
                     DHRWRGAGTVLLAQVIEAAFAAGATELDLLRGDEAYKPAWATRSRDVLRVRFGVGAAG
                     RLALSSIEALDRARAAAGRAREGIGARIR"
     gene            1396..2772
                     /locus_tag="VF954_01010"
     CDS             1396..2772
                     /locus_tag="VF954_01010"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX9529685.1"
                     /translation="MSTVWPTSGSRQELAARAAVALGLIAVSAVLAVGGVRAPGLALI
                     AVGGLAGVTLMVSRLPFALAVLVASFFFENYLSSGVGLLTPAKVIGLVAISAWLVDWA
                     VGRHRMVVTRELRWALGLLLWVVVAMSAARNEHVAVVTTLRYVSFVSLFFLVLQTVRG
                     RIKDADRLATVAMSAALAASILGLLAFFRGQVDRVSGPLEDPNDFGFLLASTLPLAVY
                     CARRAQTVMGRSAAVISLLAISIAVLLTFSRSALVGVAMAAIWALGTRRVRLRWGVAG
                     LAGLVFAGFAAYLIQPAAVSSAFSRKQHVADTNVSIRLTLWDVAWREFRSLPLTGVGP
                     GNYVSRFEEFGSTPSSDQSTFTTHNAYLNVLAELGGPGFLLFCGFLVSSWRRLRQRPE
                     GDPDTDQLLTALAAGYVVALVGSMFLTEQYYPPLWFFAALGVSMIAVPRTDEPQPVQG
                     NPGAGRGR"
     gene            2789..>3307
                     /locus_tag="VF954_01015"
     CDS             2789..>3307
                     /locus_tag="VF954_01015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9529686.1"
                     /translation="MRPSLAALTRARARLPVPRGPAVTVLGYHRVDLDGGGLAVRPAT
                     FAAQMEALDAGRDRLPVLGLAEAVGAVHGGTVRQAVVLTFDDAWADNYTHALGPLVDH
                     ALPATLYAPSRLLSTPGYLSVDQLREMSAAGVAIGAHTRTHPDLRRCTDRELLDEIRG
                     SRDDLEDLLQQPV"
BASE COUNT          412 a         1241 c         1121 g          533 t
ORIGIN      
        1 tggtcggcca cgcctacgag gctcgcgctc gggccggcgt agacgtcggc gccatggcga
       61 ccgacctgct tcgaccttcg gcggccgtgg cgctcggggg cggcgcggtt ctcctcgccc
      121 gccacgcgct ctccctcggc gccgacacct ccgtcgggcc tctgctcgtg ctcggcggca
      181 tcttcacagc ggtctacctg gcggtgtggc tgctcggggc gtcggcggtg cccgacgatc
      241 tgcgcacgct gcggtcgcgg ctcatctgat gcgcgccccg atcccttcac gggctcggcc
      301 agccgcggcc cgggcccggt cgagggcctc gatggacgac agggccagcc ggccggcggc
      361 tccgaccccg aaccgcaccc gcaggacgtc ccggctccga gtggcccacg ccggcttgta
      421 ggcctcgtcc ccccgcagga gatccagctc ggtcgcgccg gcggcaaagg cggcctcgat
      481 gacctgggcc agcagcacgg tgcccgcccc ccgccatcga tggtcgagcc gacggccggc
      541 ctggtagtag ctccatcgcc cactcagctc gaaggtcacg agggacgcgc cgacctcgtc
      601 gtcggccgcc agctcgtgaa ccgagacctc gccccggccc atcccggccg tggccgcggc
      661 cacgaaggcc ggggccgcag aggagaagcc cgagcgggag ccccactggg aagcgtgcaa
      721 ctgaacgagg cgctcgaggc tgcggcgcct cccggcgatg gcccggcaac ccgccggtac
      781 cggatccctt cgcgctcgag ccgccgcccg acccgcgcca cggtgttgcg caccgctgcc
      841 ggccggcctg cgaggtatcc ggagaactcg cccgtaagct cgacccacgg tgccccggcc
      901 accgtcgcca ctagcggccc gccccggggc ccgagtcgcg acacctgcga ggactgggcc
      961 agccccgcga cgtcgagcac cgagggccca gactgagcga accacccggt cagggcggcg
     1021 acgacctctt caccccggcc gccgagcgcc aagatgtcga ggtggtcggg gcacagagcg
     1081 ctcgggccag gttcgcggaa gcggcgcacg cccaaccatc gctcctgcga caccgccatc
     1141 ccgcctacca gccgatcgcc ctcggtcacc gtgaggtacc tacgccccgc tcctgcggtg
     1201 ccctcgagcc accacgtgcg caggaacggc gacggaaggc cggccgtcgc ggccagatcg
     1261 tcccaggccg cggcccaccc gccgagatcc cggcacagca cgacgtccac ggtgatgctc
     1321 gctccttccg gcgggcgagc cgccagtgtg cctgccgatc ggaccggcgc gtgatccggc
     1381 gtgtgatact ggcgagtgtc gacagtttgg cccacatccg ggtcccggca ggagctggcg
     1441 gcccgcgccg ctgtcgcgct ggggttgatc gcagtcagcg cggtgctcgc tgtcggaggt
     1501 gtgcgcgcac cgggactggc cctcatcgcc gtcggcgggc tcgccggcgt caccctcatg
     1561 gtcagccggc tccccttcgc cctggcggtg ctcgtggcgt cgttcttctt cgagaactac
     1621 ctgtcttcag gtgtggggct gctgactccg gcgaaggtga tcggcctggt cgccatctcc
     1681 gcctggctgg tggactgggc ggtgggccgt caccggatgg tggtgacgcg tgagttgcga
     1741 tgggcgctcg gcctgctgct gtgggtcgtc gtggccatgt cggcggccag gaacgagcac
     1801 gtcgcggtgg tgaccacatt gcggtacgtg tcgttcgtct ccctgttctt cctggtgctc
     1861 cagaccgtgc ggggtcgcat caaggacgcc gaccgcctgg ccacggtcgc catgagcgcg
     1921 gctcttgcag cctccatcct cggtctgctg gcgttcttca gaggccaggt cgaccgggtg
     1981 agcggccctc tcgaggatcc caatgacttc ggcttcttgt tggccagcac cttgccgctc
     2041 gccgtctatt gtgcccgccg cgctcagacg gtcatgggcc gctccgctgc ggtcatctcc
     2101 ttgctcgcca tctcgatcgc cgtcctgctc acgttctccc gctcggccct ggtcggcgtg
     2161 gccatggcgg ccatctgggc cctcggcacc cggcgggtgc gtctgcgatg gggcgtggcc
     2221 gggctggccg ggctggtttt cgcgggattc gcggcctacc tcatacaacc ggccgcggtc
     2281 agctccgcct tctcccgcaa gcagcatgtg gccgacacga acgtgtccat tcgtctgacc
     2341 ctgtgggatg tcgcctggcg agagttccgg tcgttgccat tgaccggtgt cgggcccgga
     2401 aactacgtgt cgcgcttcga ggagttcggg tccacgccct cgtcggacca gagcaccttc
     2461 accacccata atgcctatct caacgtcctc gccgagctcg gaggaccggg attcctcctc
     2521 ttctgcgggt tcctggtgtc ctcctggcgc cggctgcggc agcgccccga gggcgatcct
     2581 gacaccgacc agctcctcac cgcgctggcg gcgggctacg tcgtcgccct cgtcggctcg
     2641 atgttcctga ccgagcagta ctacccgccc ctgtggttct tcgccgcgct cggggtcagc
     2701 atgatcgccg tgccgcgcac cgatgagccc caacccgtcc aggggaaccc tggcgcaggg
     2761 cgcgggagat gacagcgttg gccgggccgt gaggccttcg ctcgcggcgt tgacccgagc
     2821 ccgagcgcgc ctcccggtcc cgcggggccc ggcggtcacc gtcctcggct atcaccgggt
     2881 cgatcttgac ggcggcggcc tcgccgttcg tccagccacg ttcgcggccc agatggaggc
     2941 gctcgatgcc ggtcgcgacc ggcttccggt cctcgggctg gccgaggccg tgggggccgt
     3001 acatggtgga acggtccggc aggcggtcgt cctgaccttt gacgacgcgt gggctgacaa
     3061 ctacacccac gccctgggcc cactggtgga tcacgccctt ccggccaccc tgtacgcacc
     3121 gagccgattg ttgtccacgc cgggatatct ctcggtcgac cagctcaggg agatgtcggc
     3181 cgccggggtc gccatcggcg cccatacccg cacccatccc gacctccgcc gctgcaccga
     3241 cagggagctg ctcgacgaga tcagaggcag ccgggacgac ctggaggacc tgctacaaca
     3301 accggtc
//
LOCUS       DASWGG010000060         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_27618, whole genome shotgun sequence.
ACCESSION   DASWGG010000060 DASWGG010000000
VERSION     DASWGG010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27618"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VF954_01020"
     CDS             complement(<1..1100)
                     /locus_tag="VF954_01020"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX9529687.1"
                     /translation="MLAVQGAAGARVRGRRVAVTGIGVLACCGVGKDAFWPGLFRPQL
                     EGRERRVEDFDATQWFGPKEVRRVDRFAQMAVAAASMALEDAGTPDGHGLGADPDRCG
                     VLIGTGVGGLETLEDQIQVRLEKGARRVSPFLVPMMMSNAAAATVSMRFGWRGPCETT
                     VTACAAGTHSVGNAARLIATGRCDAVMAGGSEAAVTPTAVAGFGNMTALSSSGWSRPF
                     DLRRDGFVMSEGAAVLILEEWDHAAARGARVYAEVLGAASTADAYHITAPSPGGSGAS
                     ACIELALADAELNPGDIAHINAHGTSTQLNDAAEAEAIVKVFGSPGPPITSTKGVTGH
                     ALGAAGAIEAAAAALTIAHRRMPPTAGYQVPDP"
     gene            complement(1100..1339)
                     /gene="acpP"
                     /locus_tag="VF954_01025"
     CDS             complement(1100..1339)
                     /gene="acpP"
                     /locus_tag="VF954_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9529688.1"
                     /translation="MDEKEAFAKFQDCAVEVLKVSRDQVTPDASFSEDLDADSLDLVE
                     LVMSLEEAFDITVDESELEGVNTVDAAFKLVTSKL"
     gene            complement(1413..2168)
                     /gene="fabG"
                     /locus_tag="VF954_01030"
     CDS             complement(1413..2168)
                     /gene="fabG"
                     /locus_tag="VF954_01030"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX9529689.1"
                     /translation="MSEPTGGRVVLVTGGSRGIGLACARRFQRLGDRVAVTYRSKPPA
                     GPEATADTGAELVALEPLECDVTRPDQVEAAFTAVEDRWGPVEVLVANAGVTRDGLFL
                     RMGEDAWNDVIDTNLGGVYRVARRAAAKMVRAHAGRMVFVSSSTAFTGNPGQANYAAA
                     KAGLVGLARSLARELASRNITVNVVAPGPVETDMLTALGEHRIAELTAMVPLGRAAAP
                     EEVAAAVAFLASPDAGYVTGSVLAVDGGLAMGL"
     gene            complement(2165..>2632)
                     /locus_tag="VF954_01035"
     CDS             complement(2165..>2632)
                     /locus_tag="VF954_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX9529690.1"
                     /translation="ADAAGAVVLEAAPEGQLLGWDLGVDGTAVEILDCDIGGFIHMDG
                     KEVFRRAVRAMAESARAAMERAKVGPDDIALVVPHQANARIIEAANSRIGIPMERAAM
                     VLDRTGNTSSASIPVALADAADQGRLAAGDLVLLCGFGAGMTWASAVLRWGAP"
BASE COUNT          349 a          969 c          954 g          360 t
ORIGIN      
        1 gggtccggca cctggtagcc cgccgtgggc ggcatccgcc ggtgggcgat ggtcagcgcc
       61 gccgcggcag cctcgatggc gccggcggca cccagggcgt ggccggtaac ccccttggtg
      121 gaggtgatcg gaggcccggg cgacccgaac accttgacga tggcctcggc ctcggccgcg
      181 tcgttgagct gggtcgaggt gccgtgggcg ttgatgtggg cgatgtcgcc cgggttgagc
      241 tcggcatcgg ccagcgccag ctcgatgcac gccgaggcgc cggacccgcc cggggacggc
      301 gcggtgatgt ggtaggcgtc ggcggtgctg gccgccccga gcacctcggc gtagacgcgc
      361 gcccctctgg cggcggcgtg gtcccactcc tcgaggatca gcacggccgc cccctcggac
      421 atcacgaagc cgtcccggcg caggtcgaac gggcgcgacc agcccgagga ggaaagggcc
      481 gtcatgttgc cgaagccggc caccgccgtc ggggtgacgg ccgcctcgga gccgccggcc
      541 atgacggcgt cgcaccgccc ggtcgcgatg agccgggcgg cgttgccgac cgagtgtgtg
      601 ccggccgcgc acgccgtaac cgtcgtctcg cacgggccgc gccagccgaa gcgcatggag
      661 accgtggccg cggccgcgtt ggacatcatc atgggcacga ggaacggcga cacccgccgt
      721 gcgcctttct ccagccggac ctggatctgg tcctccagcg tctcgaggcc accgaccccg
      781 gtgccgatga gcactccgca gcgatcgggg tcggcgccga gcccgtggcc gtcgggggtg
      841 ccggcgtcct ccagggccat ggacgccgcc gccacggcca tctgggcgaa ccggtccacc
      901 cggcggacct ctttgggccc gaaccactgg gtggcgtcga agtcctcgac gcggcgctcg
      961 cggccctcca actggggccg gaacaggcct ggccagaacg cgtccttgcc gacgccgcag
     1021 cacgcgagca cgccgatgcc cgtgaccgcg acgcgccggc cgcgcacccg cgcaccggcc
     1081 gctccctgga ccgcgagcat cagagcttcg aggtcacgag cttgaacgcg gcatcgaccg
     1141 tgttgacgcc ctcgagctcg gactcgtcca cggtgatgtc gaacgcctcc tcgagggaca
     1201 tcaccagctc gaccaggtcc aggctgtcgg cgtcgaggtc ctcgctgaag ctggcatcgg
     1261 gggtgacctg gtcccggctc accttcagca cctcgacggc gcagtcctgg aacttggcga
     1321 acgcctcttt ctcatccatt cccgtttcct tccctccgtt tgcggtttac aaggccctgt
     1381 cgtccaagac cctgtcgtcc aagacgctgg tgtcacaggc ccatggccag gccgccgtcg
     1441 acggccagaa cgctaccggt cacatagccg gcgtcgggtg aggcgaggaa ggccacggcg
     1501 gcggccacct cctccggtgc cgcggcccgt cccaacggca ccatggcggt cagctcggcg
     1561 atccggtgct cgccgagcgc ggtgagcatg tcggtctcga ccggtccggg cgccacgacg
     1621 ttgacggtga tgttacggct ggccagctcc cgggcgaggg accgggccag cccgacgagg
     1681 ccggccttgg cggccgcgta gttggcctgc ccggggttgc ccgtgaacgc agtggaggac
     1741 gagacgaaga ccatgcggcc ggcgtgggcc cggaccatct tcgccgcggc cctccgggcc
     1801 acccggtaca cgccgccgag gttggtgtcg atgacgtcgt tccaggcgtc ctcgcccatc
     1861 cgcaggaaca gcccgtcgcg cgtcaccccg gcgttggcca ccaggacctc gaccggcccc
     1921 caccggtcct cgacggccgt gaaggccgct tcgacctggt cgggccgggt gacgtcacac
     1981 tcgaggggct cgagggcgac gagctcggcg cccgtgtccg cggtggcttc ggggcctgcc
     2041 gggggtttgg accggtaggt gaccgcaacc cggtcgccga ggcgctggaa gcgccgggcg
     2101 caggccagcc cgatgccccg tgagccaccg gtgacgagca cgacgcgccc gcccgtgggt
     2161 tcgctcacgg ggcgccccag cgcagcacgg cgctggccca ggtcatgccg gccccgaacc
     2221 cgcacagcag caccaggtcg ccggctgcca gccggccctg gtcggcggcg tcggccaggg
     2281 ccacggggat cgaggccgag gaggtgttgc cggtgcggtc cagcaccatg gcggcgcgct
     2341 ccatcgggat cccgatgcgc gagttggcgg cctcgatgat gcgggcgttg gcctggtggg
     2401 gaaccaccag ggcgatgtcg tcgggcccca ccttggcgcg ctccatggcg gctctggccg
     2461 actctgccat ggcccggacc gcccggcgga acacctcctt gccgtccatg tggatgaagc
     2521 cgccgatgtc gcagtcgagg atctcgacag cggtgccgtc cacgccgagg tcccagccga
     2581 gcagctggcc ctcgggggct gcctccagga ccacggcccc ggccgcgtcc gc
//
LOCUS       DASWGG010000061         5041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2959043, whole genome shotgun sequence.
ACCESSION   DASWGG010000061 DASWGG010000000
VERSION     DASWGG010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2959043"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2351)
                     /gene="leuS"
                     /locus_tag="VF954_01040"
     CDS             complement(<1..2351)
                     /gene="leuS"
                     /locus_tag="VF954_01040"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX9529691.1"
                     /translation="MSEPYDAQAVEKRWQARWEAEGTYQVDADDPRPPYYVLSMYPYP
                     SGRAHMGHVRNYTFGDLMVRSMTMKGHAVLSPLGFDSFGLPAENAAIKTGVHPRLFTD
                     ARIEELKTSIKAIGAVYDWRREVRSHDPEYMRWNQVIFKRFLEAGLAYRAEAPVNWCP
                     GCQTVLANEQVLPDGTCERSGDVVTKRDLEQWFFKITDYAEELLADLEGLDWPERVKT
                     MQRNWIGRSEGAEFDLPVQGQDAALRVFTTRPDTAFGMTYAVVAVEHPMVEALATPAR
                     RPEVLAFVERVRRESDIDRQSTEGSLEKRGVFTGSHVVNPFNGQPVPVYLADYVLMGY
                     GTGAIMAVPAEDQRDWDFARVHGLPVVRTVAPPEGWEGEAYTGDGPKINSEWLDGITD
                     VPTAKARATEWLEQQGIGERKVNYRLRDWLVSRQRYWGCPIPVVYCEGDGIVAVPDEE
                     LPVLLPDDVELRPTGESPLKLHEGFRRVTCPTCGGPAERETDTMDTFVDSSWYFLRFT
                     DPWSTDGPFDKGTAAHWMPVDQYIGGIEHAILHLMYARFYTKALADVGIGPPGLREPF
                     SRLFTQGMVRLGGTRMSKSKGNLVAPEDILAGAGADALRMAHLFVGPPADDVDWETVS
                     VEGCVRFLHRVWRLGLDDVGRAVIERRPPTEDDAEIDRATHRSIQRVSDDYDRWSYNT
                     AVAALMELTNTLYRYVQAGARRETLDAAVDTLLLLAAPMAPHISAELWERRRGGNVHA
                     QPWPVADPELARLLSVTMVVQVNGKLRDKLEVDPGIDDREAERL"
     gene            2467..2739
                     /locus_tag="VF954_01045"
     CDS             2467..2739
                     /locus_tag="VF954_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529692.1"
                     /translation="MAQREKRSISLPAELAQAIEHAASSAGTTVSGWLAETAAHQLQV
                     DAGRHAVAEWEAENGPLTTEELADGLARARALLGRARPRRPAKRSA"
     gene            2739..3122
                     /locus_tag="VF954_01050"
     CDS             2739..3122
                     /locus_tag="VF954_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9529693.1"
                     /translation="MAGVTYDTGALIAAERNDRRMWALHAGFLAEEVAPCVPAPVLAE
                     AWRGGPRQANVARLLAMCEVEPMSEDQARKVGVLAGRSDHDDVVDVTVVEGAIRRGDA
                     VVTSNRDPIARVARAAGARIRIEDA"
     gene            complement(3152..3871)
                     /locus_tag="VF954_01055"
     CDS             complement(3152..3871)
                     /locus_tag="VF954_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9529694.1"
                     /translation="MSGLIEIEGLSAGYSGVAVVRDLTLVVREGEVVALLGPNGAGKT
                     TTLLTVSALLPMLAGDVRVLGRSVRRRRPHRVAGDGLAHVPEDRSLFFDLTVGENLRL
                     GTRRGGGDLAVVFDYFPALAGLLDRRAGLLSGGEQQMLTIGRALASRPRVLMVDEMSL
                     GLAPIIVERLLPVLRRFADETGCGVLLVEQHVHLALEIADRAYLLSHGTLAGHGAAKD
                     LASDRALLESSYLGEQGLHPA"
     gene            complement(3868..4671)
                     /locus_tag="VF954_01060"
     CDS             complement(3868..4671)
                     /locus_tag="VF954_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9529695.1"
                     /translation="MSLLETVDLSVTFGGLRAVDEVSIAVERGALVGLIGPNGAGKTT
                     LIDALTGFVASQGGIVFDGLHLDGLPAHRRARAGLARTWQSLELFDDLTVAENLQVAA
                     ERQGVRSFVNDVLRPSRRRDLSAVGQALASLGLSHLADRLPSALSQGHRKLVGVARAL
                     AAQPALVCMDEPAAGLDPTESHELGEQLRALTGAGTAILLVDHDMGLVLGVCDHIYVM
                     EFGQVIAHGSPREIRADERVISAYLGKTARKDIEEAKAALAARDEDRRS"
     gene            complement(4671..>5041)
                     /locus_tag="VF954_01065"
     CDS             complement(4671..>5041)
                     /locus_tag="VF954_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529696.1"
                     /translation="AAFVAGLGGALSGYRFGSVTPGTFGSIASLTFLAFAYLGGISSV
                     TGAIVGGSIVGGGITFTALQQWFHVDSRFTLLLGGLGLVVTAVRYPEGIAGALHGAGG
                     LVRSRLEARRVPDSTAAGGE"
BASE COUNT          716 a         1778 c         1839 g          708 t
ORIGIN      
        1 agccgctcgg cctcccggtc gtcgatgccg ggatccacct cgagcttgtc gcgcagcttg
       61 ccgttgacct gcaccaccat ggtcaccgac agcagccggg ccagctccgg gtcggcgacc
      121 ggccacggct gggcatgcac gttgccgccg cggcggcgct cccagagctc ggcgctgatg
      181 tgcggcgcca tgggggccgc cagcagcagc agcgtgtcga cggcggcgtc cagcgtctcg
      241 cgccgggcgc ccgcctggac gtagcggtac agggtgttgg tcagctccat cagggcggcc
      301 acggcggtgt tgtaggacca gcggtcgtag tcgtcgctca cccgctggat gctgcggtgg
      361 gtggcccggt ctatctcggc gtcgtcttcc gtcggcgggc gccgctcgat cacggcccgg
      421 cccacgtcgt cgagccccag ccgccagacc cggtgcagga agcgcacgca gccctcgacg
      481 ctcaccgtct cccagtcgac gtcgtcggcc ggcggcccca cgaagagatg cgccatccgc
      541 agggcgtccg cgccggcccc ggccaggatg tcctccggcg ccaccaggtt gcccttggac
      601 ttggacattc gcgtcccgcc caggcgcacc atgccctggg tgaacagccg gctgaagggc
      661 tcgcgcagcc cgggcgggcc gatgccgacg tcggccaggg ccttggtgta gaagcgggcg
      721 tacatcaggt gcaggatggc gtgctcgatg ccgccgatgt actggtcgac gggcatccag
      781 tgcgcggccg tgcccttgtc gaacgggccg tcggtgctcc acgggtccgt gaaccgcagg
      841 aagtaccagg acgagtccac gaaggtgtcc atggtgtcgg tctcgcgttc ggcgggcccg
      901 ccgcaggtgg ggcaggtgac acgccggaag ccctcgtgga gcttcagcgg cgactccccc
      961 gtggggcgga gctcgacgtc gtcgggcagc agcaccggca gctcctcgtc tggcacggcc
     1021 acgatcccgt cgccctcgca gtacaccacc gggatcgggc agccccagta gcgctgccgg
     1081 ctgaccagcc agtcgcgcag ccggtagttg accttgcgct ccccgatgcc ctgctgctcc
     1141 agccattccg tggcccgggc cttggcggtg gggacgtcgg tgatgccgtc gagccattcg
     1201 ctgttgatct tgggcccgtc gccggtgtac gcctcgccct cccagccctc gggcggggcc
     1261 acggtgcgca ccacgggcag gccgtggacc cgggcgaagt cccagtcgcg ctggtcctcg
     1321 gcgggcacgg ccatgatcgc cccggtgccg tagcccatca ggacgtagtc ggccaggtac
     1381 acggggacgg gctggccgtt gaacgggttc acgacgtgcg aaccggtgaa gacccccctt
     1441 ttctccaacg atccctcggt cgactggcga tcgatgtccg actcgcgccg cacccgctcg
     1501 acgaaggcca gcacctcggg ccgccgcgcc ggcgtcgcca gcgcctccac catggggtgc
     1561 tcgacggcca ccacggcgta ggtcatgccg aaggccgtgt ccggccgggt ggtgaagaca
     1621 cgaagggccg cgtcctgtcc ctgcacgggc aggtcgaact cggccccctc cgagcggccg
     1681 atccagttgc gctgcatggt cttgacccgc tcgggccagt ccaggccctc cagatcggcc
     1741 agcagctcct cggcgtagtc ggtgatcttg aagaaccact gctccaggtc gcgcttggtc
     1801 acgacgtcgc cggagcgctc gcacgtcccg tcgggcagga cctgctcgtt ggccagcacg
     1861 gtctggcagc ccgggcacca gttgaccggc gcctcggccc ggtaggccag gccggcctcg
     1921 aggaagcgct tgaagatgac ctggttccag cgcatgtact cggggtcgtg gctgcgcacc
     1981 tcccgccgcc agtcgtagac ggcgccgatg gccttgatgc tggtcttcag ctcctcgatg
     2041 cgcgcgtcgg tgaacaggcg gggatggacg ccggtcttga tggccgcgtt ctcggcgggc
     2101 aggccgaagg agtcgaaccc caacggcgac agcacggcgt ggcccttcat ggtcatggac
     2161 ctcaccatca ggtcgccgaa ggtgtagttg cgcacgtggc ccatgtgcgc ccgcccgctg
     2221 gggtaggggt acatggacag cacgtagtac ggcgggcggg ggtcgtcggc gtcgacctgg
     2281 taggtgccct cggcctccca ccgcgcctgc catctcttct cgactgcctg ggcgtcgtag
     2341 ggctcggaca tgccgtgatc gtaccgacag ggccccggtt tcctggccag ctgagctgct
     2401 ggctcgccga cgggacggtc gatccgccgg cggctgctgt cctactgcct gtgctaccgt
     2461 cgggtgatgg cccaacgtga gaagcgatcc atctcccttc cggccgagct ggcccaggcc
     2521 atcgagcacg cggccagctc ggcgggcacc acggtcagcg gatggctggc ggagaccgcc
     2581 gcccaccagc tgcaggtcga cgccgggcgg cacgccgtcg ccgagtggga ggcggagaat
     2641 ggcccgctga cgaccgagga gctcgccgac ggcttggcac gcgcccgggc gctcttgggc
     2701 cgtgcccgcc cgcgacgccc ggcgaagcgg tcggcgtgat ggctggcgtc acctatgaca
     2761 ccggggccct gatcgccgct gagcgcaacg accggcggat gtgggcattg catgccgggt
     2821 tcctggccga agaggtcgct ccgtgcgtcc ctgcgccggt gctcgcggag gcatggcgtg
     2881 gagggccccg tcaggcgaac gtggcccgcc tgctcgccat gtgtgaggtc gagccgatga
     2941 gcgaggacca ggcgcgaaag gtgggcgttc tggcgggccg gtcggaccac gatgacgtcg
     3001 tggacgtgac cgtcgtggaa ggcgccatcc gccggggcga cgccgtcgtc acgtcgaacc
     3061 gcgaccccat cgcccgagtg gcgcgagcgg ccggggcaag gatccggatc gaagacgcct
     3121 gacctccact ggccgtcggg cccactggtc ttcaggcggg gtgcagcccc tgctcgccca
     3181 ggtagctcga ttcgagcaga gcgcggtcgg acgcgaggtc cttggccgca ccgtgcccgg
     3241 ccagggtgcc gtggctgagc aggtaggcgc gatcggcgat ctcgagggcg agatgcacgt
     3301 gctgctcgac cagcaggacg ccgcagccgg tctcgtcggc gaaccgccgc agcaccggca
     3361 gcagccgctc gacgattatc ggcgccagcc cgaggctcat ctcgtcgacc atgaggacgc
     3421 ggggtcggct ggcgagggct cggccgatgg tcagcatctg ctgctcgcct cccgacagca
     3481 gcccggcccg gcggtccagc agcccggcca gggccgggaa gtagtcgaag acaacggcga
     3541 ggtcgcctcc gccgcgccgg gtgccgagcc tcaggttctc gcccacggtg aggtcgaaga
     3601 acagggaccg gtcctcgggc acgtgggcca ggccgtcgcc ggccacccgg tgggggcgcc
     3661 ggcggcggac cgatcggccg aggacgcgga cgtcgcccgc cagcatcggc aggagcgccg
     3721 acaccgtcag cagggtggtg gtcttgcccg cgccgttggg acccaggagg gcgacgacct
     3781 ccccctcgcg gacgacgagg gtcaggtcgc gcaccaccgc cacgccggag tagcccgccg
     3841 acagcccttc gatctcgatg agcccgctca tgaccggcga tcctcgtccc gcgccgcgag
     3901 ggccgccttg gcctcctcga tgtccttgcg ggcggtcttg cccaggtacg cgctgatgac
     3961 gcgctcgtcg gcgcggatct cccgggggct cccgtgggcg atgacctggc cgaactccat
     4021 gacgtagatg tggtcgcaga cgccgaggac caggcccatg tcgtgatcga ccaggaggat
     4081 ggccgtgccg gcgcccgtca aggcgcgcag ctgctcaccg agctcgtggc tctcggtcgg
     4141 gtcgagcccg gccgcgggct cgtccatgca caccagcgcc ggttgagcgg cgagagcgcg
     4201 cgccacaccc acgagcttgc gatggccctg ggacagcgca gacggcaggc ggtcggcgag
     4261 gtggctgagg cctagggacg cgagcgcttg accaacggcc gacaggtcgc ggcgccggga
     4321 gggccggagc acgtcgttga cgaacgatcg gacgccctgg cgctcggcgg cgacctggag
     4381 gttctcggcg acggtgaggt cgtcgaacag ctcgagcgac tgccaggtgc gcgccagacc
     4441 ggccctggct cgccgatggg cggggagacc gtcgagatgc aggccgtcga agacgatgcc
     4501 gccctgcgag gcgacgaacc cggtgagggc gtcgatgagg gtggtcttgc cggctccgtt
     4561 gggaccgatc agaccgacca gcgccccgcg ctcgacggcg atgctcacct cgtccacggc
     4621 ccggaggccg ccgaaggtca ccgacaggtc cacggtctcg aggagggaca tcactcgccc
     4681 cccgccgcgg tcgagtcggg gacgcgtcgg gcctctaggc gcgaccggac caggccgccg
     4741 gcaccgtgca gggcgccggc gatgccctcc gggtagcgca cggcggtgac caccagcccg
     4801 agcccaccga gcaggagcgt gaaccggctg tcgacgtgga accactgctg gagggcggtg
     4861 aaggtgatgc cgccgcccac gatggagccc ccgacgatgg cgccggtgac gctcgagatg
     4921 ccgccgagat aggcaaaggc gaggaacgtc agcgaggcga tgctcccgaa cgtgccaggg
     4981 gtcaccgacc cgaaccggta gcccgacagc gcccctccca gcccggcgac gaaggcggcc
     5041 a
//
LOCUS       DASWGG010000062         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1024453, whole genome shotgun sequence.
ACCESSION   DASWGG010000062 DASWGG010000000
VERSION     DASWGG010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024453"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1541)
                     /gene="glgP"
                     /locus_tag="VF954_01070"
     CDS             complement(<1..>1541)
                     /gene="glgP"
                     /locus_tag="VF954_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX9529697.1"
                     /translation="VEAVRAGTVFTTHTPVPAGIDRFPREMMERYFRAFADECGVSFN
                     DLMALGHEPGAGADSAFNMAVMGLRLASMSNGVSKLHARVSQKMFSGLWPGVPEAEAP
                     IQAVTNGVHARTWVSAEMDELLNRYVLPEWPEADDTRWMHIQDANDDEVWRVKEQGRQ
                     RLVAHIRERLRHSALARGVSESDVAWTNEVLDPRVLTLGFARRFAAYKRATLLLSQPE
                     RLKALLTSAERPIQIVFAGKAHPADDIGKEMIRQIIAFARLPEVRHRMAFVEDYDMVV
                     ARRMYQGCDVWMNTPRRPLEACGTSGMKAAINGSLNCSILDGWWDEMFDGENGWAISS
                     AETYEDLAKRDEVEAESLFDLLERQIVPLFYERYGGPVPRRWVRRVKASLRSLGPQVS
                     ASRMVRDYVEEMYEPAATRVDTLAAGGGERAKALSAWKQALARAWPAVRIISVDTEST
                     LVDLGASKKVEAVVALGQLHQHDVEVQLLHGPVGANEELQPAAIVTMDLVGLDEPGPG
                     DGGEA"
BASE COUNT          212 a          566 c          529 g          234 t
ORIGIN      
        1 tggcctctcc gccgtcaccg ggcccgggct cgtccagccc caccaggtcc atggtcacga
       61 tggccgccgg ttgcagctcc tcgttggcgc cgaccgggcc gtggaggagc tggacctcca
      121 cgtcgtgctg gtggagctga ccgagggcca ctaccgcctc gaccttcttc gacgccccca
      181 ggtcgaccag cgtcgactcg gtgtcgaccg agatgatccg caccgccggc caggcccggg
      241 ccagggcctg cttccaggcc gagagggcct tggcccgctc accgccgccg gcggccagcg
      301 tgtcgaccct ggtggcggcg ggctcgtaca tctcctccac gtagtcgcgc accatccgtg
      361 aggcgctgac ctgggggccc agcgaacgca acgacgcctt cacccgccgt acccaccgcc
      421 ggggcaccgg cccgccgtag cgctcgtaga acagggggac gatctggcgc tcgaggaggt
      481 cgaagaggct ctcggcctcg acctcgtcgc gcttggccag gtcctcgtag gtctcggccg
      541 agctgatggc ccagccgttc tccccgtcga acatctcgtc ccaccagccg tcgaggatcg
      601 agcagttgag cgaaccgttg atggccgcct tcatgccgct ggtgccgcag gcctccagcg
      661 gccggcgggg cgtgttcatc cacacgtcgc agccctggta catgcggcgg gccaccacca
      721 tgtcgtagtc ctcgacgaag gccatccggt ggcggacctc gggcaggcgg gcgaaggcga
      781 tgatctggcg gatcatctcc ttgccgatgt cgtcggcggg gtgggccttg ccggcgaaga
      841 cgatctggat gggccgctcc gccgacgtga gaagggcctt gagccgctcc ggctgcgaca
      901 gcaggagcgt ggcccgcttg taggccgcga accggcgggc gaagcccagg gtcagcaccc
      961 gggggtcgag gacctcgttc gtccaagcca cgtcggactc gctcaccccc cgggccaggg
     1021 cggagtgccg cagccgctcc cggatgtggg ccaccagccg ctgccggccc tgctccttga
     1081 cccgccacac ctcgtcgtcg ttggcgtctt gaatgtgcat ccagcgggtg tcgtcggcct
     1141 ccggccactc cggcaggacg tagcggttga gcagctcgtc catctcggcc gacacccacg
     1201 tccgggcgtg cacgccgttg gtgaccgcct ggatcggcgc ctcggcctcc ggaacgccgg
     1261 gccacagccc gctgaacatc ttctggctga cccgggcgtg gagcttcgac acgccgttgg
     1321 acatcgaggc cagccggagg cccatcaccg ccatgttgaa ggcggagtcg gccccggccc
     1381 ccggctcgtg gcccagggcc atgaggtcgt tgaagctgac gccgcactcg tcggcgaagg
     1441 cccggaagta ccgctccatc atctcccggg ggaaccggtc gatcccggcc gggacgggcg
     1501 tgtgggtggt gaagaccgtg ccggcccgca ccgcctccac g
//
LOCUS       DASWGG010000063         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2959898, whole genome shotgun sequence.
ACCESSION   DASWGG010000063 DASWGG010000000
VERSION     DASWGG010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2959898"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VF954_01075"
     CDS             <1..245
                     /locus_tag="VF954_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529698.1"
                     /translation="AVVPEDQMVAALVDYAERIMADGAAAVLAGADRDGARRAAEADR
                     AALLDEKGADVNHSEERVELIRKSMSAEPMSGESPA"
     gene            285..1706
                     /gene="proS"
                     /locus_tag="VF954_01080"
     CDS             285..1706
                     /gene="proS"
                     /locus_tag="VF954_01080"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX9529699.1"
                     /translation="MAKPPVLTPQAADFPRWYQDVVAKAELADNGPVRGTMVIRPYGY
                     AIWELMQADLDRRIKAAGAANAYFPLFIPESYLRKEAEHVEGFSPELAVVTIAGGKEL
                     EEPVVVRPTSETIINSFFAKWVSSYRDLPLLINQWSNVVRWELRPRLFLRTTEFLWQE
                     GHTAHATEADARSYALQILTDVYRDFMDTVLAIPVVIGRKTESERFAGATVTWTCEAM
                     MGDGRALQMATSHELGQNFARVFGTSYTDARGAEEHVWQTSWGASTRLMGALIMAHGD
                     DQGLRLPPAIAPVQVVVLVVREEPGVAEAAASLMADLVSSGVRAVLDDRLDAGFGRRS
                     VDWEIKGAPLRVEVGPRDLAAGQLTLVRRDAGTKEPIPLEGAVARVLLALESAQAGLR
                     ATALERQSRATVAVTSVEEAAEAAGAGQWATLPWAAVGVDGELRLKERQATVRCLLAE
                     DGSLPVAGEADEASLVAVVAKAY"
     gene            1832..>2093
                     /locus_tag="VF954_01085"
     CDS             1832..>2093
                     /locus_tag="VF954_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX9529700.1"
                     /translation="MDPATRVRELIEPALVSAGFELVDVEASSHLVRVTVDRAGGGID
                     LDAVSAASRLVSGLLDAHDPVRGDAYTLEVSSPGLERRLRTPE"
BASE COUNT          286 a          738 c          777 g          292 t
ORIGIN      
        1 tggccgtggt gcccgaggac cagatggtcg cggccctggt cgactacgcc gagcgcatca
       61 tggcggacgg cgccgcggcc gtgctcgccg gggccgaccg ggacggggcc cgccgggcgg
      121 ccgaggccga tcgcgccgcc ctgctcgacg agaagggggc ggacgtgaac cacagcgagg
      181 agcgggtgga gctgatccgc aagtccatgt ccgcggaacc aatgtccgga gaaagcccgg
      241 cctgatccgg gcggccgccg cgccggggcc tagcatctcc cgccatggcg aagccgcccg
      301 tgctcacccc ccaagccgct gatttccccc gttggtacca ggacgtggtg gccaaggccg
      361 agctggccga caacgggccg gtgcgcggga cgatggtcat ccgcccctac ggctacgcca
      421 tctgggagct catgcaggcc gacctcgacc ggcggatcaa ggccgccggc gccgccaacg
      481 cctacttccc gctgttcatc ccggagtcct acctgcggaa ggaggccgag cacgtcgagg
      541 gcttcagccc cgagctggcc gtggtcacca tcgccggcgg caaggagctg gaggagcccg
      601 tcgtggtccg cccgaccagc gagaccatca tcaactcgtt cttcgccaaa tgggtctcca
      661 gctaccggga cctgcccctg ctcatcaacc agtggtccaa cgtggtgcgc tgggagctgc
      721 ggccccggct gttcctgcgc accaccgagt tcctctggca ggagggccac accgcccacg
      781 ccaccgaggc cgacgcccgc tcctacgcgc tgcagatcct gaccgacgtg taccgggact
      841 tcatggacac ggtcctggcc atcccggtgg tcatcgggcg caagaccgag agcgagcgct
      901 tcgcgggcgc caccgtgacc tggacctgtg aggccatgat gggcgacggc agggccctcc
      961 agatggccac gagccacgag ctgggtcaga acttcgcccg ggtgttcggg acgtcgtaca
     1021 ccgacgcgcg gggcgccgag gagcacgtct ggcagacctc ctggggggcg tcgacgcggc
     1081 tcatgggcgc cctgatcatg gcccacggcg acgaccaggg gcttcgcctg ccccccgcca
     1141 tcgccccggt ccaggtggtg gtgctggtcg tgcgcgagga gccgggcgtg gccgaggcgg
     1201 ccgcctcgct gatggccgac ctggtgtcgt cgggggtgcg ggccgtgctg gacgaccggc
     1261 tcgacgcagg cttcggccgg cggtcggtcg actgggagat caagggtgcg ccgttgcggg
     1321 tggaggtcgg gcctcgcgac ctggctgccg ggcagctgac cctggtgcgg cgggacgcgg
     1381 ggaccaagga gccgatcccg ctggagggcg cggtcgcacg ggtcctcctc gccctggagt
     1441 cggcccaggc cggcctgcgt gccaccgccc tcgaacggca gtcacgcgcc acggtggcgg
     1501 taaccagcgt ggaggaggcg gccgaggcgg ccggggccgg ccagtgggcc accctcccgt
     1561 gggcggccgt cggcgtcgac ggcgagctgc ggctcaagga gcgacaggcg accgtgcggt
     1621 gcctgctggc cgaggacggc tcgctgcccg tggccggcga ggcggacgag gcctcgctgg
     1681 tcgccgtggt ggccaaagcc tattgacgcc agcctgctga ttgccgccga ttgctatact
     1741 cgccgagcca ttcggttcga gagaggcggt ggtcgtgcgt gggcctgttg cccacgcttt
     1801 tgcttgatcg gtccttgacg agaggaggca gatggacccg gcaacccgtg tccgtgagct
     1861 catcgagccc gccctcgtct ccgccggctt cgagctggtg gacgtcgagg cgtccagcca
     1921 ccttgtgcgg gtcacggtcg accgggcagg aggcggcatc gacctcgacg ccgtgagcgc
     1981 ggcctcgcgg ctggtgtccg gcctgctgga cgcccacgac cccgtccggg gcgacgccta
     2041 caccctcgag gtctccagcc ccggcctgga gcgccggctg cggacgccgg agc
//
LOCUS       DASWGG010000064         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1672125, whole genome shotgun sequence.
ACCESSION   DASWGG010000064 DASWGG010000000
VERSION     DASWGG010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672125"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VF954_01090"
     CDS             complement(<1..653)
                     /locus_tag="VF954_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9529701.1"
                     /translation="MTALMQTYAPAPVTFVRGAGTELWDGDGKRYLDFLCGLAVTSLG
                     HCHPEVADAVANQARTLLHVSNLFDNAVGPEVAGTLDRLLGGGGQVFFCNSGAEANEC
                     AIKLARRWGGPGRYVVVSAYNSFHGRTLATLHATGQPAKHEIFQPLPEGFRHVAWDDL
                     GALETSLDASVVAAVLLESVQGEGGVNPASAAYLQGVRRICDHRGVLLMIDEVQTGLG
                     "
     gene            complement(650..1513)
                     /gene="argB"
                     /locus_tag="VF954_01095"
     CDS             complement(650..1513)
                     /gene="argB"
                     /locus_tag="VF954_01095"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX9529702.1"
                     /translation="MTAPLTAADKAAVLAEALPYIRRFWGQVVVVKYGGNAIGDHESL
                     ALFAQDVVLMRSVGMRPVVVHGGGPQIGELMARLGKVPEFREGLRVTDADTLDIARMV
                     LVGKVNRDLVAAINVHAPLAVGLSGEDAGMITATARDPALGFVGDVGLVDASILDRIL
                     AQELIPVVATIGADLEGQSYNINADTAAGAVAAAVGAEKLVYLTNVEGLLADVDDPAS
                     LIRTTTADELDHLLEQGSVSAGMIPKLRSCVEAIRAGVGHAHILDGRVPHALLLEIFT
                     REGVGTMVSPS"
     gene            complement(1510..>1728)
                     /locus_tag="VF954_01100"
     CDS             complement(1510..>1728)
                     /locus_tag="VF954_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX9529703.1"
                     /translation="VSFDPDRVEVAYGDITVCRDGVAAPHDGAALAAYMGGRKLAIRC
                     DLRLGAGRGVILTNDLSPGYIDENMRTS"
BASE COUNT          249 a          607 c          634 g          238 t
ORIGIN      
        1 cccagcccgg tctggacctc gtcgatcatc aggagcacgc cgcggtggtc gcagatgcgg
       61 cgcaccccct ggaggtaggc ggccgacgcc gggttgacgc caccctcgcc ctgcaccgat
      121 tcgagcagca cggcggcgac cacggacgcg tccaggctcg tctccagggc gccgaggtcg
      181 tcccacgcca cgtgccggaa gccctcgggc aggggctgga agatctcgtg cttggccggc
      241 tgcccggtgg cgtgcagggt ggccagcgtc cggccgtgga acgagttgta ggcgctgacc
      301 accacgtagc ggcccggccc gccccagcgg cgggccagct tgatggcgca ctcgttggcc
      361 tcggccccgg agttgcagaa gaacacctgg cccccgccgc ccagcagccg gtcgagggtg
      421 ccggccacct ccggccccac ggcgttgtcg aacagattgg acacgtgcag cagcgtccgc
      481 gcctggttgg ccacggcgtc ggccacctcg gggtggcagt ggccgagcga ggtcaccgcc
      541 aggccgcaca ggaagtcgag atagcgcttt ccgtcgccgt cccacagctc ggtcccggcg
      601 ccgcggacga aggtgaccgg ggcgggtgca taggtctgca tcagcgctgt catgacgggc
      661 tgaccatggt gcccacccct tcacgggtga agatctcgag gagcagggcg tgcggcaccc
      721 gcccgtccag gatgtgggcg tggccgacgc cggcgcggat ggcctcgacg caggaccgca
      781 gcttggggat catcccggcg gacaccgagc cctgctcgag caagtggtcg agctcgtcgg
      841 cggtcgtggt gcggatgagg ctggccgggt cgtcgacatc ggccaacagg ccctccacat
      901 tggtcaggta gaccagcttc tcggcgccga cggcggcggc gacggcaccg gccgcggtgt
      961 cggcgttgat gttgtaggac tggccttcga gatcggcgcc gatcgtggcc accacgggga
     1021 tcagctcctg ggccaggatg cggtccagga tcgacgcgtc gaccaggccg acgtcgccga
     1081 cgaagccgag cgccgggtcc cgcgccgtgg cggtgatcat gccggcgtcc tcgccggaga
     1141 ggcccacggc cagcggggcg tgcacgttga tggccgccac caggtcgcgg ttgaccttgc
     1201 ccaccagcac catgcgcgcg atgtccaggg tgtcggcgtc ggtaacccgc agaccctcgc
     1261 ggaactccgg caccttgccc agccgggcca tcagctcgcc gatctgcggc ccgcccccgt
     1321 ggacgaccac cggccgcatg ccgaccgaac gcatcagcac gacgtcctgg gcgaacaggg
     1381 cgagggactc gtggtctccg atggcgttgc cgccgtactt caccaccacc acctgtcccc
     1441 agaagcgccg gatgtagggc agggcctcgg cgagcacggc ggccttgtcg gcggcggtca
     1501 acggggcggt cacgaggtcc tcatgttctc gtcgatgtag ccgggcgaca ggtcgttggt
     1561 gaggatgacc ccgcggccgg caccgaggcg gaggtcgcag cggatggcca gcttccgccc
     1621 gcccatgtag gcggccaggg cggcgccgtc gtggggggcc gcgacgccgt cccggcagac
     1681 ggtgatgtcg ccgtaggcga cctcgacccg gtcggggtcg aagctcac
//
LOCUS       DASWGG010000065         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2960412, whole genome shotgun sequence.
ACCESSION   DASWGG010000065 DASWGG010000000
VERSION     DASWGG010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2960412"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VF954_01105"
     CDS             <1..505
                     /locus_tag="VF954_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9529704.1"
                     /translation="FKHLSVRENLLLGRHIHYGANGLQGVLRTGRARAAERGAEEHVE
                     AVAARCGLTPLLDAPAGDLPYGTQRMVEVARALAAEPSVLLLDEPGAGMDPTESDYFG
                     DLLLQIHDGHHSVVLIEHDVPMVLRVCDRIYVLQFGKLIAQGTPDEIRANAAVREAYF
                     GSAVEVA"
     gene            505..1248
                     /locus_tag="VF954_01110"
     CDS             505..1248
                     /locus_tag="VF954_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9529705.1"
                     /translation="MSALPVVDVEPLLSVRGLEVAYGPVSAVRGLTLEVHDGEIVALL
                     GSNGAGKTSSLRGITGLITPRAGRVTFAGRRLDTAGPGTAVAEGMAHVPEGRRIFRDL
                     AVADNLALGAWGRGRSAESERTQMVYDMFPRLAERRRQLAGSLSGGEQQMLAVGRALM
                     SGPKLLLIDELSLGLSPLVTDELLADLVKLNKEGLAIVLIEQFVHRALGVADRVYLLA
                     KGQLAWVGTPAEAVRTGAVEAAYLHGGSR"
     gene            1245..>1879
                     /locus_tag="VF954_01115"
     CDS             1245..>1879
                     /locus_tag="VF954_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529706.1"
                     /translation="MSSVRLPTGPARRRRILRGVAGVALTGTALMLAVPSGAGLPPDV
                     RVIDSHASAAAVGVLSRVPAESDGGAIYSSTALTVDKTQAQAAGFTAGDLAELFFESS
                     SDKYRNPALVRAQYPPAGTVPAESSFDGAAAPAGAPATGGAGHLHAVAAETPSAAAEA
                     AGARAALGDVTVGGGTSASSGELGADGTLITKARSAATDIRIAKVLTVANAT"
BASE COUNT          226 a          740 c          651 g          262 t
ORIGIN      
        1 gttcaagcac ctgtcggtgc gggagaacct cctcctcggc cggcacatcc actacggcgc
       61 caacggtctc cagggcgtgc tgcgcaccgg ccgggcccgg gccgccgagc ggggggcgga
      121 ggagcatgtc gaggcggtcg ccgcccggtg cgggctcacc cccctcctcg acgcccctgc
      181 cggcgacctg ccctacggca cgcagcggat ggtcgaggtc gcccgggcgc tggcggccga
      241 gccgtcggtc ctgctgctcg acgagcccgg cgccggcatg gacccgaccg agtcggacta
      301 cttcggcgac ctgctcctcc agatccacga cggccaccac tcggtcgtcc tcatcgagca
      361 cgacgtcccc atggtgctgc gggtctgcga ccgcatctac gtcctgcagt tcggaaagct
      421 gatcgcgcag ggcacaccgg acgagatccg ggccaacgcc gcagtccgcg aggcctactt
      481 cggttccgcc gtggaggtgg cgtgatgagt gctctgccgg tcgtcgacgt cgagcctctg
      541 ttgtcggtgc ggggcctcga ggtcgcctac ggtcccgtgt cggccgtgcg gggcctcacc
      601 ctcgaggtcc acgacggcga gatcgtcgct ctgctcggct ccaacggcgc cgggaagacc
      661 tccagcctgc gggggatcac cggcctgatc accccccggg ccgggcgggt caccttcgcc
      721 ggacggcgtc tcgacaccgc cggccccggc acggcggtgg ccgagggcat ggcccacgtc
      781 cccgagggtc ggcgcatctt ccgggacctg gccgtggccg acaacctcgc cctcggggcc
      841 tggggccggg gccggtcggc ggaatccgag cggacccaga tggtctacga catgttcccc
      901 cgcctggccg agcggcgccg ccagctggcc gggtcgctgt ccggcggtga gcagcagatg
      961 ctggccgtcg gccgggccct catgtccggt ccgaagctgc tgctgatcga cgagctgtcg
     1021 ctcggtctgt ccccgctggt gaccgacgag ctcctggccg acctggtgaa gctgaacaag
     1081 gaaggtctgg ccatcgtgct catcgagcag ttcgtccacc gggccctcgg cgtcgccgac
     1141 cgcgtctacc tgctggccaa ggggcagttg gcctgggtcg gcacgccggc cgaggccgtc
     1201 cgcaccggtg cggtggaagc cgcctacctc cacggaggat cgagatgagc tccgtccgtc
     1261 tgcccaccgg cccagcccgg cgccgtcgca tccttcgcgg cgttgcgggc gtcgccctga
     1321 ccggcaccgc gctgatgctg gccgtgccgt cgggggccgg tctcccgccc gacgtgcggg
     1381 tcatcgactc ccacgcctcg gccgccgccg tcggtgtcct gtcccgggtg ccggccgaat
     1441 ccgacggggg cgccatctac tcctccaccg ccctcaccgt cgacaagacc caggcccagg
     1501 ccgccgggtt caccgccggc gacctcgccg agctgttctt cgagtcctcc agcgacaagt
     1561 acaggaatcc ggcgctggtc cgggcccagt acccgccggc cggcacggtg cccgccgaga
     1621 gctccttcga cggcgccgcc gccccggcgg gcgccccggc caccggcggg gccggccacc
     1681 tccacgccgt ggccgccgag accccttcgg ccgccgccga ggccgccggc gcccgggccg
     1741 ccctgggcga cgtcaccgtc ggcgggggca cctcggcgtc gtcgggtgag ctcggcgccg
     1801 acggcaccct catcacgaag gcccgctccg ccgccaccga catccgcatc gccaaggtcc
     1861 tcaccgtcgc caacgccac
//
LOCUS       DASWGG010000066         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_352216, whole genome shotgun sequence.
ACCESSION   DASWGG010000066 DASWGG010000000
VERSION     DASWGG010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352216"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VF954_01120"
     CDS             <1..337
                     /locus_tag="VF954_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529707.1"
                     /translation="TSGHGRGITTINTTWQVLHDALSQRRAVRARYHDRLRVLCPHAL
                     GWKNGRPKVLVYQTAIVGESSTHDPRGWRSLFVDEIHDAVISDDRWESANNYTQGNNG
                     IDTISAAIR"
     gene            487..732
                     /locus_tag="VF954_01125"
     CDS             487..732
                     /locus_tag="VF954_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529708.1"
                     /translation="MRFRLGFALGAALGYYLGAMAGQERYEQINRMLKRAGRSQAFDV
                     ATGKAKAVVDLGVERARDVVETKIGGGLTETRERERL"
     gene            complement(737..2158)
                     /gene="rpoD"
                     /locus_tag="VF954_01130"
     CDS             complement(737..2158)
                     /gene="rpoD"
                     /locus_tag="VF954_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX9529709.1"
                     /translation="MTEGAPTLSTPAGVAPEEFVLLLAQGRSRGFLTQDDLMEVLRRV
                     ELTPDVIAAVIDRIRHEGIVFEDEPDVVLDADLEAIAAEAEEEAAGPAAAPPPSLPGP
                     VADGPGPVANGLVGDAGAALDEPPPPVPLTPVAALPARLKSSRHRPPSTEGRLLDDDG
                     RPGLGADSVRTYLKEIGKVRLLSAESEVGLARRIETGLAARDDLVLLEDRCGGVDVPR
                     LERRPLDRSIRDGREAKDLLIQANLRLVVSIAKRYRNRGMAFLDLIQEGNVGLMRAVD
                     KFDYTKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKMVRVQRQLLQEMG
                     REPTVEELALRAEMTAERVLEIQRISLDTISLNEPMGDDEDFSLSDVIEDQAAIVPAD
                     AATRALLNVAVKDALSQLTEREQEVMRLRFGLDDGQMRTLEEVGKVFGVTRERIRQIE
                     SKTLAKLRHPMRSQPLRDYLDAE"
     gene            complement(2155..>2885)
                     /locus_tag="VF954_01135"
     CDS             complement(2155..>2885)
                     /locus_tag="VF954_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529710.1"
                     /translation="RARAAEAAIATVAEHPSDLVRDQYLMQVADRCRVEPERLRAMLN
                     QARRAPATAGPARSRPATSRVDRSPLGERELPGDPSGGGPGPGRPQLRAAAAEPSGPE
                     LEALRMALHHPEAVVERLEILLFTDETAMAAFRALASANTLHEALAVADPDASALLGR
                     LAVEEPDAEPDDVVNLLIRRAAERALADLQVEARVDPERAQQLLELTAWVKVNIEQLL
                     EPETGVDAGRRLVAWLAERCEEEA"
BASE COUNT          437 a         1026 c         1012 g          410 t
ORIGIN      
        1 cacttcgggc catggtcggg gcatcaccac gatcaacacc acctggcagg tcctccacga
       61 cgccctcagc caacgtcgcg ccgtccgggc ccgataccac gaccggctcc gagtcctgtg
      121 cccccacgcc ctcggctgga agaacggccg gcccaaggtt ctcgtgtacc agaccgccat
      181 cgtcggcgag agctctacac acgatcctcg aggatggcga tcactgttcg tcgacgagat
      241 ccacgatgcc gtcatcagcg atgaccgctg ggagagcgcc aacaactaca cgcagggcaa
      301 caacggcatc gacaccatct ccgccgccat caggtgacca caaccccaaa ccacacagcc
      361 ggcaaatccc gaacgagatg tgcggaaagc gagccccatg agcaccaggt taccaaacgg
      421 gtgttcgtca tcgccggccg cgggggtcgg ggtgcgccgg agacgccgaa ggggtacaaa
      481 gccagcatga gatttcgtct tgggttcgcg ctgggcgccg ccctggggta ctacctcggg
      541 gccatggccg gacaggagcg ctacgagcag atcaaccgga tgctcaagcg agccggccgg
      601 tcacaggcgt tcgacgtggc caccggcaag gccaaggcgg tcgtggacct cggggtggaa
      661 cgggcgcggg atgtggtcga gaccaagatc ggcggcggtc tcaccgagac acgggagcga
      721 gaacgactgt agggagctac tcggcgtcca ggtagtcgcg gagcggctgg ctgcgcatgg
      781 ggtggcgcag cttggccagg gtcttggact cgatctggcg gatgcgctcg cgcgtcacgc
      841 cgaagacctt gccgacctcc tccagcgtcc gcatctgccc gtcgtccagc ccgaaacgca
      901 ggcgcatgac ctcctgctcc cgctcggtca gctgcgagag agcgtccttc acggccacgt
      961 tgagcagcgc tcgggtggcc gcgtccgccg gtacgatcgc cgcctggtcc tcgatgacgt
     1021 ccgagaggga gaagtcctcg tcgtcgccca tgggctcgtt cagcgagatc gtgtccaggc
     1081 tgatgcgctg gatctcgagg acccgctccg ctgtcatctc ggcgcgcagg gccagctcct
     1141 cgactgtggg ctcgcgcccc atctcctgga ggagctggcg ctgcaccctg accatcttgt
     1201 tgatggtctc caccatgtgc accgggatcc ggatggtgcg cgcctggtcg gcgatggccc
     1261 gcgtgatggc ctggcgtatc caccaggtgg catacgtcga gaacttgaag cccttggtgt
     1321 agtcgaactt gtcgaccgcc cgcatgagcc cgacgttgcc ctcctggatg aggtccagaa
     1381 aggccatgcc gcggttgcgg tacctcttgg cgatggacac gacgagccgc aggttggctt
     1441 ggatcagcag gtccttggcc tcgcgaccgt cgcggatcga gcgatcgagg ggccggcgct
     1501 cgagtcgcgg cacgtcgacc ccgccgcacc ggtcctccag cagcaccagg tcgtcgcggg
     1561 cggccaggcc ggtctcgatc cgacgggcga ggcccacctc ggactccgcg gacagcaggc
     1621 ggaccttgcc gatctccttg agataggtgc ggaccgagtc ggcgcccaag ccgggacgac
     1681 cgtcgtcgtc gaggagccgc ccctcggtcg aagggggccg gtggcgagac gacttgagcc
     1741 tggccggcag cgccgcgacc ggcgtgagcg gcaccggcgg cgggggctcg tcgagggcgg
     1801 cgccggcgtc cccgaccaac ccgttggcga ccgggccggg gccgtccgcg acaggaccgg
     1861 gcagggatgg cggcggggcg gcggccggcc cggccgcctc ctcctcggcc tccgcggcga
     1921 tggcctccag gtccgcatcg agcacgacgt cgggctcgtc ctcgaagacg atgccctcgt
     1981 gccggatgcg gtcgatcacc gcggcgatca cgtcgggcgt gagctcgacc cgccgcagga
     2041 cctccatgag gtcgtcctgg gtgaggaagc cacgcgaccg gccctgcgcc agcaggagga
     2101 cgaactcctc cggcgccact ccggctggcg ttgagagcgt gggggcgccc tcggtcatgc
     2161 ttcctcctcg caccgctcag caagccaggc taccaaccgc ctgcccgcgt cgacgccagt
     2221 ctccggctcg agcagctgct cgatgttgac cttcacccag gcggtgagct cgagcagctg
     2281 ctgggcccgc tcgggatcga cccgcgcctc cacctgcagg tcggccaacg cccgctccgc
     2341 cgcccgccgg atgagcaggt tgaccacgtc gtcgggctcg gcgtcaggct cctctacggc
     2401 cagtcggccg agcagcgccg acgcgtcagg gtccgccacg gcgagcgcct cgtgaagggt
     2461 gttggccgag gccagggccc ggaacgcggc catcgccgtc tcgtcggtga acaggaggat
     2521 ctccaggcgc tccaccactg cctccgggtg gtggagggcc atgcgcaacg cctccagctc
     2581 cgggccgctc ggctcggcgg cggccgcccg gagctgcggc cgaccggggc ccggacctcc
     2641 gccggagggg tccccaggca gctcccgctc gccgagcggc gaccggtcga cccgcgaggt
     2701 agccggccgg ctgcgcgccg gaccggcggt cgcgggagcg cggcgggcct ggttgagcat
     2761 ggcccgcagc cgctcgggct cgacccggca cctatcggcc acctgcatga ggtactgatc
     2821 ccggacgagg tcgctagggt gctcggcgac cgtggcgatg gcggcctccg cggcccgggc
     2881 ccgcc
//
LOCUS       DASWGG010000067         2616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1674996, whole genome shotgun sequence.
ACCESSION   DASWGG010000067 DASWGG010000000
VERSION     DASWGG010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674996"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VF954_01140"
     CDS             <1..839
                     /locus_tag="VF954_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9529711.1"
                     /translation="PVVVALGARRDDEVGLAASSRGGAYVGLRARGGVVGVLALEWRG
                     RPPASPGPAEVLEGVREHAALALDNARWFARLRMLGASERAHIAHDLHDNLGQSLAYL
                     GIELERLGRRTSDGALQAEITRLRETVRGVIGDLRDTLHDLRTDVSAEVDLPATLSTV
                     ALRLHERSGLQVTVDNQAERRLALLREREVWRIAYEALVNATRHSAASSVNVSWRCDA
                     SGFELDVDDDGAGFDPDSLAAVRKGGLAAMRARADAIGARLEVHSRPGAGTRVVCSGA
                     AA"
     gene            836..1495
                     /locus_tag="VF954_01145"
     CDS             836..1495
                     /locus_tag="VF954_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9529712.1"
                     /translation="MTSVLLVDDHRLLRESLRRTLEESGLEVAGEAANGNEAVRMAGE
                     LQPDVVLMDVSMPVLDGVEATRLIRRQSPDVRVVMLTMHAEDEVLTRAVRAGAAGYLV
                     KDCSAQDILDAVTRVCSGGTTLSIDVASAMLAEATRVSPTPDGQDVRSGSGPIITRRE
                     EEVLQLIAEGFSTSEVGQRLFISAKTVKNHLASVYEKLDSRDRTQAVVRAVRMGIIRL
                     D"
     gene            1667..1864
                     /locus_tag="VF954_01150"
     CDS             1667..1864
                     /locus_tag="VF954_01150"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX9529713.1"
                     /translation="MDIVTYTKTIILPYLRARFDRGEEGAAMVEYGLLVALIAVVCVA
                     AITLIGTALNGKFGDIVTSLK"
     gene            complement(2048..>2616)
                     /locus_tag="VF954_01155"
     CDS             complement(2048..>2616)
                     /locus_tag="VF954_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9529714.1"
                     /translation="AASDARRRDTPSTTPRQDMPTLPRVTIVRWPEEAAVAAGLRDTG
                     SPRLLLVAPDAAAPEALHWDEDWVRLPARDDDVRARTAALQARAAARAPRPEVTGDGR
                     LSYAGRWVQLSPIEEPMARLLADRFGDVVDAGSLAACAAGRRLSATAVRVHITRLRKR
                     VKTMELSIRVVRGHGYVLDADPSEPVPE"
BASE COUNT          369 a          949 c          929 g          369 t
ORIGIN      
        1 aaccggtcgt cgtcgccctg ggcgctcgtc gcgacgacga ggtcgggctg gccgcctcgt
       61 cgcggggcgg cgcctacgtc gggctccggg cgcggggcgg cgtcgtcggt gtcctcgccc
      121 tcgagtggcg aggccgaccc cctgccagtc cgggaccggc cgaggtgctc gaaggcgttc
      181 gggagcacgc cgccctcgcc ctggacaacg cccggtggtt cgcccggctg cgcatgctgg
      241 gagcgagcga gcgggcccac atcgcccacg acctgcacga caacctgggc cagtcgctcg
      301 cctacctcgg catcgagctc gaacgcctgg gccgcaggac gtccgatggg gcgctacagg
      361 ccgagatcac ccgcctgcgg gagacggtcc ggggtgtgat cggcgatctc cgcgacaccc
      421 tgcacgatct gcgcaccgac gtgtcggccg aggtcgacct tccggccacc ctctccacgg
      481 tcgccctccg gctccacgag cggtccggcc tgcaggtcac cgtcgacaac caggccgagc
      541 gccgcctcgc cctgctgcgg gagcgcgaag tgtggaggat cgcctacgag gccctcgtca
      601 acgccacccg gcattcggcg gccagctccg tgaacgtgtc gtggagatgc gacgcctccg
      661 gcttcgagct cgacgtcgac gacgacggcg ccggcttcga ccccgactcc ctcgcggccg
      721 tgaggaaagg cggcctggcc gccatgcggg cccgcgccga cgccatcgga gcgcggctcg
      781 aggtccacag ccggccgggc gccgggacga gggtcgtctg ctcgggagcg gcggcatgac
      841 cagcgtcctg ctcgtcgacg accaccggct gctgcgcgaa tcgctccggc gcaccctgga
      901 ggagtccggg ctggaggtgg cgggcgaggc cgccaacggc aacgaggcgg tgcgcatggc
      961 cggcgagctc cagcccgacg tggtgctcat ggacgtctcc atgcccgtgc tcgacggtgt
     1021 cgaggccacc cgcctgatcc gccggcagtc gcccgacgtg cgggtcgtaa tgctcaccat
     1081 gcacgccgag gacgaggtcc tgacccgcgc cgtgcgcgcc ggggcggcgg gctacctggt
     1141 caaggactgc tccgctcagg acatcctcga cgcggtcacc cgggtctgct ccggtgggac
     1201 cacgctgtcc atcgacgtgg cctcggcgat gctggccgag gccacgcggg tgtccccgac
     1261 ccccgacggc caggacgtgc ggtccgggag cgggcccatc atcacccggc gggaggagga
     1321 agtcctccag ctgatcgccg agggattctc gacctccgag gtgggacagc ggctgttcat
     1381 aagcgccaag acggtgaaga accatttggc gtcggtgtac gagaagctcg actcccgtga
     1441 ccggacgcag gccgtggttc gggccgtgcg aatggggatc atccgcctgg actgagggcg
     1501 cggcgcgaca gtcggctcgg gtgcccccgg tggtggggcc tcatattcgc gccccccata
     1561 ttccccgcta caagtctaaa tgagtcgatc ggcctatgga gtcaatgacc cgcaaggccg
     1621 aagatagata cagcccccgg tccccccaaa cagcaaggag catcccatgg acatcgtgac
     1681 ctataccaag accatcatcc tcccctacct cagggcccgc ttcgaccgcg gtgaggaggg
     1741 tgccgccatg gtcgagtacg gcctgctggt cgccctcatc gccgtcgtct gtgttgccgc
     1801 catcaccctc atcgggacgg cgctcaacgg aaagttcggc gacatcgtaa ccagcttgaa
     1861 gtagttccct tccgcagtct ctccttggtc agacaagtgg gcgggcctcc gggcccgccc
     1921 actgtcgcgt ccctatggcg tccgccctct cttgcccggc ggcccccgga acaggcgccg
     1981 cacggccagc ttgacggccg tggcgacggc gaccagcccg atgaacccca acacggcagc
     2041 catcagatca ctccgggacc ggctcgctcg ggtcggcatc gaggacgtag ccgtggccgc
     2101 gcacgacccg gatgctgagc tccatggtct tgaccctctt gcggagccgg gtgatgtgga
     2161 cgcgcaccgc ggtcgcgctg agacggcggc cggcggcgca agccgccagg ctgcccgcgt
     2221 ccacgacgtc gccgaagcgg tcggccagca gccgcgccat gggctcctcg atcggtgaga
     2281 gctgcaccca ccgaccggcg taggagaggc gcccgtcgcc ggtgacctcg ggccgcggcg
     2341 cccgggccgc ggcgcgggcc tggagggcgg cggtgcgggc gcgcacgtcg tcgtcccggg
     2401 ccgggagccg gacccagtcc tcgtcccagt gcagcgcttc gggcgctgct gcgtcaggcg
     2461 cgaccagcag caggcggggc gatccggtat cccgcaggcc ggcggccacc gccgcctcct
     2521 ctggccatcg gacgatcgtg actcggggga gcgtgggcat gtcttgccgg ggcgtcgtgg
     2581 acggggtgtc gcggcggcga gcatcgctgg ccgccg
//
LOCUS       DASWGG010000068         2930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1352199, whole genome shotgun sequence.
ACCESSION   DASWGG010000068 DASWGG010000000
VERSION     DASWGG010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352199"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /locus_tag="VF954_01160"
     CDS             complement(<1..1745)
                     /locus_tag="VF954_01160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX9529715.1"
                     /translation="MRPLSTRHMAFLMGVVLLAATAAAGGRGSAWLTSALPGPAAPAS
                     APVTATVDPGLPRTGAQPVRVIVQGAPGQAVAARQAAAAVGAQIGTDLPIVDGFGATL
                     PADRISRLARAAGVRAITADRQATFQSFSFDTTTAASNFVATSGAGQAWSQGLYGDGV
                     GVAVIDTGVSPHNDLSGRIVWGPDLSGEGKIIDSYGHGTVMAGIIGGNGADSASNPGG
                     AYTGVAPHATVVAVKTAGYNGTVDVSTILQAMHWVAAYQSQFNIRVLNLSWGVASTQD
                     PSSDPLDYAVERLWSQGIVVVVAAGNSGPQAGTITKPADDPVVLTVGAYDDKQNTDPA
                     DDSIPSWSSQGPTAQGLAKPDVVTPGRYIIATRSLGSYVETTFPKALRAPSYIRGSGT
                     SEASAMTSGLAALLVEAHPGWTPDQVKYALTSTASPIAGFTANAQGAGRVQLASALSA
                     NASAAPAQTLAATGLGSIEASRGSQHVQTVCPQDNQTHVIQGEIDVRCEQWSGSAWTG
                     SAWTGSAWTGSAWTGSAWTGSAWTGSAWTGSAWTGSAWTGSAWTGSAWTGGTWSGSAW
                     TGSAWTGSAWTGSAWT"
     gene            2139..2330
                     /locus_tag="VF954_01165"
     CDS             2139..2330
                     /locus_tag="VF954_01165"
                     /inference="COORDINATES: protein motif:HMM:NF020512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HEX9529716.1"
                     /translation="MAKAGDRIELPGRKVGQARRTGVVEDVHGSLLTVQWDTGERTTF
                     TPAAGALQVLAPRPRRKAR"
     gene            complement(2379..>2930)
                     /locus_tag="VF954_01170"
     CDS             complement(2379..>2930)
                     /locus_tag="VF954_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529717.1"
                     /translation="THPASSPPPTSARSGPTASTPSSTVPASPATLVDLADPTGDQGK
                     DGPAYADGLRLQLESSGDQLTATVTMAATLPAKLASSEVMGIGVDLYRPGGSESDYQL
                     FADGEPDGWYAYLDTPTGFTRYPGTFDLVQSRVVFTVPWSALGDIRSGQTSAFVDWSR
                     NGATTSVASADHIPDAGRVSFSR"
BASE COUNT          408 a         1002 c         1115 g          405 t
ORIGIN      
        1 gtccatgcgc taccggtcca tgcgctgccg gtccatgcgc tgccggtcca cgcactgccc
       61 gaccaggtgc cgccggtcca ggcgctgccc gtccaggcgc tgcccgtcca ggcgctgccc
      121 gtccaggcgc taccggtcca ggcgctgccc gtccaggcgc taccggtcca ggcgctaccg
      181 gtccaggcgc taccggtcca ggcgctgccc gtccaggcgc tacccgtcca ggcgctgccc
      241 gaccactgct cgcagcggac gtcgatctcg ccctggatga cgtgggtctg gttgtcctgc
      301 gggcagaccg tctgcacgtg ctggctgccg cgggacgcct cgatcgagcc caggccggtc
      361 gcggccaggg tttgcgccgg ggcggcactg gcgttggcgc tgagcgcgct ggccagctgg
      421 acccggcccg ccccttgagc gttggcggtg aagccggcga tcgggctggc ggtcgaggtg
      481 agggcgtact tgacctggtc cggggtccag cccggatggg cctcgaccag cagggcggcc
      541 aggccgctgg tcatggcgga ggcctcagag gtcccgctgc cccggatgta gctcggggcc
      601 cgcagcgcct tggggaaggt cgtctcgacg taagagccca gcgagcgggt ggcgatgatg
      661 taacggcccg gggtcaccac gtccggcttg gcgaggccct gggcggtcgg cccctgcgag
      721 gaccaggacg ggatcgagtc gtcggccggg tcggtgttct gcttgtcgtc gtaggcgccg
      781 acggtgagca cgaccgggtc gtcggccggc ttggtgatgg tgccggcctg cgggccgctg
      841 ttgccggccg ccaccactac cacgatgccc tgcgaccaca gacgctcgac ggcgtagtcc
      901 aacgggtcgg agctggggtc ctgggtcgag gccacgcccc acgagaggtt gaggacccgg
      961 atgttgaact ggctctggta ggccgccacc cagtgcatgg cctggaggat ggtggacacg
     1021 tcaaccgtgc cgttgtagcc ggctgtcttg accgcgacca cggtggcgtg gggggcgaca
     1081 cctgtgtagg cgccaccggg gttggaggcc gagtcggcgc catttccccc gatgataccg
     1141 gccatgaccg tgccgtggcc gtaggagtcg atgatcttgc cctcgccgga gaggtcgggg
     1201 ccccagacga tgcgaccgga caggtcgttg tggggggaga ccccggtgtc gatgacggcc
     1261 acgccgaccc cgtcgccgta gaggccctgg ctccacgcct ggcccgcccc cgaggtggcc
     1321 acgaagttgg aagccgcggt ggtcgtgtcg aagctgaagg actggaacgt cgcctggcgg
     1381 tcggcggtga tggcccgcac gcccgccgcc cgggccagcc gggagatgcg gtcggccggc
     1441 agcgtggccc cgaagccgtc gacgatcggc aggtcggtgc cgatctgggc gccgaccgcg
     1501 gccgcggcct ggcgggcggc gactgcctgg ccgggcgcgc cctggacgat cacccggacc
     1561 ggctgggcac ccgtgcgggg caggccagga tcgaccgtcg ccgtcaccgg ggcgctcgcc
     1621 ggcgccgccg ggccgggcag cgctgacgtc aaccaggccg agccgcggcc cccggccgcg
     1681 gcggtggccg cgagcagcac cacccccatc aggaacgcca tgtggcgcgt cgacagcggg
     1741 cgcacccgcc agagtgaagg gctcggcacg gagagtgaag tggaggtcac ccatgaagtg
     1801 gtacgccgtc gcgccgctgc tggtaatgac gaaagggagc catttggtcg gcgccgacgg
     1861 gctagtcatg gggggcataa cgggcatctc ctgccaaagc gccccgccaa ccactgcaag
     1921 tggtggtcta gccctcacga agagtggtaa tgcccccagg tgtgactagt ccatcgccag
     1981 gcaccggctg ggcgaaccgg ccgagctgac ccattgctcc ccgagacctg gccccaacgg
     2041 gccgtcccgc cgctggcacg ccggcccggc cgggtagtaa ccggcaaacg gaggggtagc
     2101 gtgagcccat cacacgccga gcgacggagg tgccgctgat ggccaaggcc ggtgaccgaa
     2161 tcgagcttcc tgggcgaaag gtgggccagg cccggcggac gggcgtcgtg gaagacgttc
     2221 acggctccct gctcacggtg cagtgggaca ctggcgagcg gaccacgttc acgccggccg
     2281 cgggggcgct ccaggtgctc gcaccccggc cccggcgcaa ggcccggtag ccgtccggag
     2341 ccgccggctc ggctgcccag gggctgctcc gcggctgctc agcggctgaa cgacacccgc
     2401 cccgcatcgg ggatgtggtc cgcagacgcc accgacgtcg tggccccgtt gcgtgaccag
     2461 tcgacgaagg cactggtctg gccggaccgg atgtcaccga gcgcactcca cggaacagtg
     2521 aacacgacgc ggctctggac caggtcgaac gtcccgggat agcgggtgaa cccggtgggg
     2581 gtgtcgaggt aggcgtacca cccgtccggc tcgccgtcgg cgaagagctg gtagtcgctc
     2641 tcgctcccac ccggccggta gaggtcgacg ccgatgccca tgacctccga gctggcgagc
     2701 ttcgcaggca gggtcgcggc catggtcaca gtggcggtga gctggtcgcc cgaggactcc
     2761 agctggagcc tcaacccgtc ggcgtacgca ggcccgtcct tgccctggtc gccagtaggg
     2821 tcggccaggt cgacgagggt cgccggcgac gcgggcaccg tcgaggaggg ggtcgaggcg
     2881 gtcgggccgg atcgggccga cgtcggaggt ggtgacgatg cggggtgggt
//
LOCUS       DASWGG010000069         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2288550, whole genome shotgun sequence.
ACCESSION   DASWGG010000069 DASWGG010000000
VERSION     DASWGG010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2288550"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VF954_01175"
     CDS             complement(<1..511)
                     /locus_tag="VF954_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9529718.1"
                     /translation="MPPGPVPISSAAPAAPAADALAGAAELAAAMAEVLGGVLRGKPD
                     VIRLSLAALFSGGHLLIEDVPGVGKTLLAKAMARSIDATFRRIQATPDLLPGELTGVS
                     VFATDRDGWDFRPGPLFAHVVLVDEVNRATPRTQSALLEAMEERQVTVDGVTHPLPAP
                     FFLVATQNPF"
     gene            553..>2281
                     /gene="glgX"
                     /locus_tag="VF954_01180"
     CDS             553..>2281
                     /gene="glgX"
                     /locus_tag="VF954_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX9529719.1"
                     /translation="MVLWPGSPFPLGAHFDGAGTNFSVFSEVAEGVELCLFDAGGEET
                     RHAMSEVTAFCWHGYLPNVGPGQRYGFRAHGPFARDQGLLCNPAKLLLDPYAKAVDGE
                     VRWDEALYNHHFADPGGARNDRDSAPFLPKSVVTSPYFDWSGDRSPQTPWHETVVYEV
                     HVKGFTQRHPDIPEELRGTYGGLAHPAATEYLANLGVTAVELLPVHQFVHDAHLLERG
                     LRNYWGYNSIGFLAPHNAYCSSGQDGQQVQEFKQMVKALHEAGLEVILDVVYNHTAEG
                     NHLGPVLSFKGLDNAAYYRLVPEDRRYYYDTTGTGNSLNMRHPQVLQLIMDSLRYWVS
                     DMHVDGFRFDLAATLARQFHEVDRLSAFFDLIHQDPVISRVKLIAEPWDLGEGGYQVG
                     NFPALWSEWNGRYRDSVRDYWRGEDQRVDDFAYRLTGSPDLYEGTGRRPSASVNFVTA
                     HDGFTLADLVAYNDKHNEANGEANRDGEGHNRSWNCGVEGPTTDPGVLECRGRRRRDL
                     LATLLLSHGIPMLLGGDEMGRTQGGNNNAYCQDNEISWVDWSRAAGERDLLAFTRRVA
                     RLRRHHPVFR"
BASE COUNT          351 a          835 c          789 g          306 t
ORIGIN      
        1 cgaaggggtt ctgggtggcc accaggaaga acggcgccgg caggggatgg gtcaccccgt
       61 ccaccgtcac ctggcgctcc tccatggcct cgagcagcgc ggactgggtg cggggggtgg
      121 cccggttgac ctcgtcgacg agcacgacgt gggcgaagag cgggccgggg cggaagtccc
      181 agccgtcgcg gtccgtggcg aagacggaca cgcccgtcag ctcgccgggc aggaggtccg
      241 gggtggcctg gatccgccgg aacgtggcgt cgatgctgcg ggccatggcc ttggccagca
      301 gcgtcttgcc caccccgggc acgtcctcga tcaggaggtg gccgccggag aacagggccg
      361 cgagcgacag ccggatgacg tccggcttgc cccgcaggac cccgccgagc acctcggcca
      421 tggccgcggc cagctcggcg gcgccggcga gggcatccgc ggcgggcgcg gcgggcgcgg
      481 cggagctgat cggcacgggt ccaggcggca cgggtccaat tgtggaccct cgggggcgga
      541 tagggtcccc gggtggttct ctggcccggg tcccctttcc ccctgggcgc ccacttcgac
      601 ggcgccggca ccaacttctc ggtcttctcg gaggtggccg agggggtcga gctgtgcctg
      661 ttcgacgccg gcggtgagga gacccggcac gccatgtccg aggtgaccgc cttctgctgg
      721 cacggctacc tgcccaacgt cggccccggc cagcggtacg ggttccgggc ccacggcccc
      781 ttcgcccggg accaggggct gctctgcaac ccggccaagc tgctcctcga cccctatgcc
      841 aaggccgtcg acggcgaggt gcgctgggac gaggccctgt acaaccacca cttcgccgac
      901 cccggcggcg cccgcaacga ccgcgacagc gccccgttcc tgcccaagtc cgtggtgacc
      961 agcccgtact tcgactggtc cggcgatcgg agcccgcaga cgccgtggca cgagaccgtc
     1021 gtctacgagg tccacgtcaa gggcttcacc cagcgccatc cggacatccc cgaggagctg
     1081 cgcggcacct acggcgggct ggcccacccg gccgccaccg agtacctggc caacctgggg
     1141 gtgaccgccg tggagctgct gccggtgcac cagttcgtgc acgacgccca cctgctggag
     1201 cggggcctgc gcaactactg gggctacaac tccatcggct tcctggcccc ccacaacgcg
     1261 tactgctcga gcggccagga cggccagcag gttcaggagt tcaagcagat ggtcaaggcc
     1321 ctccacgagg ccggcctcga ggtgatcctc gacgtggtct acaaccacac ggccgaaggc
     1381 aaccacctcg ggccggtgct gtcgttcaag gggctcgaca acgcggccta ctaccggctc
     1441 gtgcccgagg accggcgcta ctactacgac accacgggga ccggcaacag cctcaacatg
     1501 cgccatcccc aggtccttca gctcatcatg gactccctgc gctactgggt cagcgacatg
     1561 cacgtcgacg gcttccgctt cgacctggcc gccacgctcg cccgccagtt ccacgaggtg
     1621 gaccggctgt cggcgttctt cgacctgatc caccaggacc cggtgatcag ccgggtgaag
     1681 ctgatcgccg agccgtggga cctgggcgag gggggctacc aggtcggcaa cttccccgcc
     1741 ctgtggtccg agtggaacgg ccgctaccgc gacagcgtgc gcgactactg gaggggagag
     1801 gaccagagag tcgacgactt cgcctaccgc ctgaccggca gccccgacct ctacgaggga
     1861 accggccggc ggccctcggc cagcgtgaac ttcgtgaccg cccacgacgg cttcaccctc
     1921 gccgacctgg tggcctacaa cgacaagcac aacgaggcca acggggaggc caaccgggac
     1981 ggcgagggcc acaaccggtc ctggaactgc ggcgtcgaag ggccgaccac cgatcccggc
     2041 gtgctcgagt gccggggccg ccggcgccgg gacctgctcg ccacgctcct gctctcccac
     2101 ggcatcccga tgctcctcgg cggggacgag atgggccgca cgcagggggg caacaacaac
     2161 gcctactgcc aggacaacga gatctcctgg gtggactggt cccgtgcggc gggcgagcgc
     2221 gacctgctcg ccttcaccag gcgcgttgcc cggctgcggc ggcaccaccc ggtgttccgc
     2281 c
//
LOCUS       DASWGG010000070         3073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1033781, whole genome shotgun sequence.
ACCESSION   DASWGG010000070 DASWGG010000000
VERSION     DASWGG010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033781"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VF954_01185"
     CDS             complement(<1..291)
                     /locus_tag="VF954_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529720.1"
                     /translation="MTAPAEVLWLSPELGPDARAGGQIRTTRLLQALGRHATVDVVAV
                     QPWTIDSHVTAQRANARTVITARRRHPVGARLLAVRHGWPIAGGALWRGDIAS"
     gene            complement(288..1301)
                     /locus_tag="VF954_01190"
     CDS             complement(288..1301)
                     /locus_tag="VF954_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529721.1"
                     /translation="MSVAGRVTLQVNLAPADLPHARLLLGHQLRQLGGQVDEILLVVD
                     RERGAGRFADGWDEGRPLLDELLATAARGDARIRVQAVDYGADAAAAISRRFLRGRPA
                     PRKDFRGGPFYSYLYGLHACATDLVLHIDSDMLFGGGSQTWVRDATAVLEGDARVVAA
                     SPLPGPPSPSGRLLSQRAERYPEAGKDAYRFDKLSTRVFLASISRLERTILPIGSTAA
                     APRRRLEARLRGSEGAGLPEDLLSRAMCRGGFRRVDLLGPGPGLWSLHPRYRPPAFYA
                     ALPELIERVERGDVPDGQRGRHDIAPCLVPLPARRDRGRIHQLSELARACRAPRPVPR
                     GPR"
     gene            complement(1303..2394)
                     /locus_tag="VF954_01195"
     CDS             complement(1303..2394)
                     /locus_tag="VF954_01195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9529722.1"
                     /translation="MEVAIVHDYFTQRGGAERVAIVMARAFPDAVVHTSVVDVAGTFP
                     DAPFERMRPSPLNHLGPLRTRHRALLPLLAPVFSSMVVDAPVVVCSSSGWAHGVRATG
                     RKLVYCHNPARWLYQTTDYVRGHRLQALALRSLAPSLRRWDRKAAASADLYVANSTVV
                     ARRIEAAYGRHAEVLFPPVSLDASAPQESLGLECGFLLCVSRLMPYKNLDAAIEGVAG
                     SGRRLVVVGDGPERARLAALARGRADVAILHRVSDAQLRWLYQHCAAVLAPSIEDYGL
                     TPLEAAGFGQPTLALRRAGYLDTVREGVTGLFFDRPTQEHVRRAVRELPRHRFDAGVL
                     RAHAARFGEGPFVRRLQGLVSSLAAGERD"
     gene            complement(2510..>3073)
                     /locus_tag="VF954_01200"
     CDS             complement(2510..>3073)
                     /locus_tag="VF954_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX9529723.1"
                     /translation="FGTWPFFVQERIGAGGRLLRFPKLRTLPPETPAYTLKRDLDTAS
                     LPWLARALRRSHLDELPQLFLVLGGQLSLVGPRPKMPDEAEPVPSRYGMVRASVPQGC
                     TGLWQISRASHLLPHEAPEFDFFYVSNRGLRLDCWILWRTALVVTGLGRRVDLHDVPM
                     WCRRRPGAQVWNGHVGPMSRVGGSNPA"
BASE COUNT          450 a         1091 c         1177 g          355 t
ORIGIN      
        1 cgaggcgatg tcgccgcgcc agagcgcccc gccggcgatg ggccagccgt gccggacggc
       61 gagcagccgg gcgccaaccg gatgccggcg gcgagcagtg atgacggtcc gggcgttggc
      121 ccgctgggcc gtcacgtgcg agtcgatggt ccagggctgc acggcgacca cgtccaccgt
      181 cgcgtgacgg cccagcgcct ggagcaggcg ggtggtgcgg atctggccgc cggccctggc
      241 gtcgggtccg agctcggggc tgagccagag cacctcggcg ggcgcggtca acgcggccct
      301 cgtggtaccg ggcgcggcgc ccggcacgcc cgggccagct ccgacagctg gtgaatgcgt
      361 ccgcggtcgc gcctcgccgg caggggcacc agacacggcg cgatgtcgtg gcggccccgc
      421 tgcccgtcgg ggacgtcgcc gcgctccacc cgctcgatga gctcggggag ggcggcgtag
      481 aaggccggtg gccggtagcg gggatgcagg gaccacaggc cgggccccgg gccgaggaga
      541 tcgacgcggc ggaagccacc ccggcacatg gcgcggctca gcaggtcctc gggcaggccg
      601 gcgccctcgg acccccgcag ccgcgcctcc agcctgcgcc ggggggcggc ggccgtcgag
      661 ccgatcggca ggatggttcg ctcgagccgg gagatggacg ccaagaacac cctggtgctc
      721 agcttgtcga agcggtacgc gtccttcccc gcctctgggt agcgctcggc ccgttgcgac
      781 agcagcctcc ctgagggcga cggcggcccg ggcagggggc tggcagccac gacgcgggcg
      841 tcgccctcga gcaccgctgt cgcgtccctc acccacgtct gcgaacctcc gccgaagagc
      901 atgtccgagt cgatgtgcag gaccaggtcg gtggcgcagg cgtggaggcc gtagaggtag
      961 gagtagaagg gacccccgcg aaagtccttg cgcggtgccg gccggccgcg caggaagcgg
     1021 cgggagatgg ccgcagcagc gtcggccccg tagtcgacgg cctggacccg gatgcgggca
     1081 tcgcctcgcg ccgccgtggc gagcagctcg tcgaggagag gccggccctc gtcccagccg
     1141 tccgcgaacc gccccgcgcc gcgctcgcgg tcgaccacca ggaggatctc gtcgacctga
     1201 ccgccgagct ggcgcagctg gtggcccagc aggagccggg cgtgcggaag gtccgccggc
     1261 gccaggttga cctgcagggt cacccggccc gcaacgctca tctcagtcgc gctccccggc
     1321 ggccaggctg ctgaccaggc cttggaggcg ccgcacaaag gggccttcgc cgaagcgggc
     1381 ggcgtgggcg cggagcacac cggcatcgaa tcgatgccgg ggcagctctc ggaccgctcg
     1441 ccggacgtgc tcctgggtgg gccggtcgaa gaacaggccg gtcacgccct cccgcacggt
     1501 gtcgaggtac ccggcccggc ggagggcgag ggtcggctgc ccgaaccccg ccgcttccag
     1561 cggcgtgagc ccgtagtcct cgattgaggg ggcgagcacg gcggcgcagt gctggtacag
     1621 ccaccgcagc tgggcgtcag agacgcggtg gaggatggcc acgtcggcgc gtcccctcgc
     1681 caacgccgcc agcctcgccc gctccggccc gtcgcccacg acgacgagac gccggccgga
     1741 gccggcgacg ccctcgatag cggcgtcgag gttcttgtag ggcatcagcc gcgagacgca
     1801 gaggagaaag ccgcactcca ggccgagcga ctcctgcggg gcgctggcgt ccagcgacac
     1861 cggggggaag agcacctcgg cgtggcggcc gtacgcggcc tcgatccgcc gggccacgac
     1921 ggtcgagttg gccacgtaga gatcggcaga cgcagccgcc ttccggtccc atctccgcag
     1981 cgacggcgcg agcgaccgga gggccagagc ctgcagcctg tggccccgca cgtagtccgt
     2041 ggtctggtag agccagcggg ccgggttgtg gcagtagacg agcttcctgc cggtggcgcg
     2101 cacgccgtgg gcccagccgc tggagctgca gaccaccacc ggcgcgtcga ccaccatgga
     2161 cgagaagacc ggcgccagca acggcaacag ggcgcgatgg cgcgtccgca acgggcccag
     2221 gtgattgagc ggcgacggcc gcatgcgctc gaagggggca tccgggaagg tcccggccac
     2281 gtccacgacc gacgtgtgga cgactgcgtc cgggaaggcc cgagccatca cgatcgccac
     2341 ccgctcggcg ccgccccgct gggtgaagta gtcgtggacg atcgccacct ccaccgtcgc
     2401 cgacttcggc gcggcagggc ggcggggcgc gacggcgtcg agcgcgtcta cgtcctggcc
     2461 actccgcggc cgcgtcgtga cgggcgctgc cggtgccggg ggtacgagct caagcagggt
     2521 tggacccccc gacgcggctc atcgggccga cgtggccgtt ccacacctgc gcgcccgggc
     2581 ggcgccggca ccacatcggc acgtcgtgga gatcgaccct gcggcccagg ccggtgacaa
     2641 cgagcgcggt gcgccagagg atccagcagt cgagccggag gccacggttg ctcacgtaga
     2701 agaagtcgaa ctcgggcgct tcgtggggta gcaggtggct ggcccggctg atctgccaca
     2761 gaccggtgca cccctgcggc accgacgccc ggaccattcc gtaccgggat ggcaccggct
     2821 cagcctcatc aggcatcttc ggccgggggc ccaccaggct gagctgaccg ccgaggacga
     2881 ggaagagctg ggggagctcg tcgaggtggc tccggcgaag cgcccgtgcc agccacggca
     2941 gcgaggccgt gtcgagatcg cgcttgagcg tgtaggccgg cgtctcgggc gggagggtgc
     3001 gaagcttggg gaacctgagg agccgacctc cagcgccaat ccgctcttgc acgaagaacg
     3061 gccaggtccc gaa
//
LOCUS       DASWGG010000071         1829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1355644, whole genome shotgun sequence.
ACCESSION   DASWGG010000071 DASWGG010000000
VERSION     DASWGG010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355644"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..994)
                     /locus_tag="VF954_01205"
     CDS             complement(170..994)
                     /locus_tag="VF954_01205"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEX9529724.1"
                     /translation="MNPRPTATATYPHGADLELIPFHQGPDRDECSFLLTDELARHDG
                     LLYGGTAAAATVMAMEAATQRDAIWVLTQFVAQTGVGERITVVVRKLAEGSHVAQLQV
                     TATAGERLIFCALGATGHARPNGLTGQFDTMPTVTPPEASRPVSHGRPERTQHGANPN
                     LEYREATYEPSLPPGRIPLWARLTAGRDLTRAAIAYLADRVPRAITRGAGRMQPDAAQ
                     LFTAWPPGRVRAGVGSVAASGNVNSIAPPLMTRSRIDSATTESEPANTSPRPGSRP"
     gene            complement(1019..>1829)
                     /locus_tag="VF954_01210"
     CDS             complement(1019..>1829)
                     /locus_tag="VF954_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529725.1"
                     /translation="ASTPGFTGADLANVLNEAALLAVRRKAATIAGEDLAEAVERVVS
                     GPRRRGRLMTDGELTRLAYHEAGHAVVAAAIGLAGQLRRISIVARGRNAAHTDITRTD
                     RTLLTRDELHAELAVLMAGVAAEEIAADQPSTAGEADVERATELARDIVGRFGMSPAV
                     GRIRVLGRQAEVFLGRDYLSTQHLSSQTLARMDEAVCALIEEAEQRARALVSERRAAL
                     DALADALLDHETVDETELADVLGQEVLSGPSPGRSRRRPRSEVTPRPVAAE"
BASE COUNT          244 a          633 c          676 g          276 t
ORIGIN      
        1 acccaccacg acgtcgtctt gtctgctgta gcgggacagc aggacgccga gggcgccgag
       61 caccgtcatg aacaaggtgc agttgtgctc ctggctgatc gagcgcagcc tctgggcggt
      121 atcgccaggc accctcaagg aagtgaaacg agccgaccgc tcggcgatat cacggccgac
      181 tgcccggccg aggacttgta ttcgctggtt ccgattcggt cgtggccgag tcgatcctgg
      241 accgcgtcat caacggcggc gcaatcgaat tcacattccc ggacgcggct accgacccaa
      301 cgccggcccg cacccgcccc ggaggccaag ccgtgaagag ctgcgccgcg tccggctgca
      361 tcctcccggc gccccgcgtg atggcccggg gcacccggtc ggcgaggtag gcgatggctg
      421 cccgggtgag gtccctgccg gccgtcagac gggcccacag gggaatccgc ccgggtggca
      481 ggctcggctc gtaggtggct tcgcggtact ccaggttcgg gttggctccg tgctgggtcc
      541 gctcgggccg gccgtggctc accggccggc tggcctcggg aggcgtgacc gtcggcatgg
      601 tgtcgaactg gccggtcagc ccgttggggc gggcatggcc ggtcgcgccg agggcgcaga
      661 agatcaatcg ctcgcctgcg gtggcggtca cctggagctg ggccacgtgg ctgccctcgg
      721 ccaatttgcg cacgaccacc gtgatccgct caccgacccc ggtctgggcg acgaactggg
      781 tgagcaccca gatggcgtct cgctgcgtgg cggcctccat ggccatcacc gtggccgccg
      841 cggccgtgcc gccgtagaga agcccgtcgt ggcgggcgag ctcgtcggtg agcaagaagg
      901 agcactcgtc ccggtcgggc ccctggtgga acgggatgag ctcgaggtcg gcgccgtggg
      961 ggtaagtggc cgtcgcggtc ggtcgcgggt tcattgggtc accctctgtg ccgctgcctc
     1021 actcagccgc gacgggacga ggggtcacct cgctccgagg ccgacggcgg gagcgacccg
     1081 ggctgggccc ggagaggact tcctgcccga ggacgtcggc cagctcggtc tcgtccacgg
     1141 tctcgtggtc gagcagagcg tcggccaggg cgtccagcgc ggctcggcgc tcggagacca
     1201 gcgcgcgcgc ccgctgctcg gcttcctcga tgagggcgca cactgcctcg tccatgcgtg
     1261 cgagggtctg gctggacagg tgctgggtgg acaggtagtc gcggccgagg aaaacctcgg
     1321 cctggcggcc cagcactcga atccgcccga cggcagggga catgccgaac cggccgacga
     1381 tgtcgcgggc cagctcggtg gctcgctcga cgtcggcctc ccctgccgtg ctcggttggt
     1441 ccgcggcgat ctcctcggcg gcgacacccg ccatcagcac ggcgagctcg gcgtgcaact
     1501 cgtcgcgggt caacagggtc cggtcggtgc gggtgatgtc cgtgtgcgcg gcgttgcgcc
     1561 cccgggccac gatggagatc cgccggagct ggccggcgag accgatggcg gcagccacga
     1621 ccgcgtggcc cgcctcgtga taggcgaggc gtgtgagctc accgtcggtc atgagccgtc
     1681 cgcgccgtcg aggaccggac accacccttt cgaccgcctc ggccagatcc tctccggcga
     1741 tggtcgccgc tttgcgccgc acggccagga gcgcggcctc gttcaacacg ttcgccaggt
     1801 ccgcgccggt gaagccgggg gtggacgcc
//
LOCUS       DASWGG010000072         6428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_713498, whole genome shotgun sequence.
ACCESSION   DASWGG010000072 DASWGG010000000
VERSION     DASWGG010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713498"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VF954_01215"
     CDS             <1..537
                     /locus_tag="VF954_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529726.1"
                     /translation="HVGIGFDAAVVDQVERRSHLKRYAGHPLFVYAAFATWFRHYDRS
                     RPHLTLALEDPGALGDGGRAVPGYFAICLNSTPYTFLGSRRLNLAPDAAFDQGLVVLN
                     FRTIRFWPVLGVLASALGSGDRIRHHRRVDYHRDVGRAVVTASGEPLPYQVDGDYVGE
                     VDRLVVEHEPEALRLVMP"
     gene            1085..1375
                     /locus_tag="VF954_01220"
     CDS             1085..1375
                     /locus_tag="VF954_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX9529727.1"
                     /translation="MALTWSRSIDWDTDDWRTRAACRDTDPDLFFPVGTTGPAIEQID
                     AARAVCRECDAHAQCLEFALATNQESGIWGGTSEEERRALRKAWLARQRRTA"
     gene            complement(1463..2962)
                     /locus_tag="VF954_01225"
     CDS             complement(1463..2962)
                     /locus_tag="VF954_01225"
                     /inference="COORDINATES: protein motif:HMM:NF023702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9529728.1"
                     /translation="MPQDAATVATTPSELGRTYTDLSEPELAHLERLMASWGMLADLC
                     FADLLLFVPAQGQGSGQFVVLGQMRPTTGQTLHRQDLVGNLIDEAERPLVARAWHLGQ
                     LVEGETTTAGRTERARMQCIPVRWNGRLLAVLTREAPLTVGRRTGELERVYVEIFDRF
                     AQMIVRGEFPFVGDEAEISEAPRVGDGVLVLDAGGRIEYTSPNAVSALHRVGIHGNAE
                     GATLQELGVGETAVARAFLTAVAVTEEIESRAEVTVLVQCLPLLHNGVVSGALVLLQD
                     VTALRRRDRLLVSKDATIREVHHRVKNNLQTISSLLRLRGRRLEPGPGKDALEDVERE
                     IRSIAVVHEILSREVTEQVPSAEILRSLVRMAEDTARSLGKVVTFSTVGDAGELPAQV
                     VTPLAVVLNELLQNAVEHAFNDDDGAAEPDGGDNRIDVRVDREGGDLVVQVRDNGRGL
                     PDGFSLERTASIGLSIVREMVRSQLGGELAMVNARGALVELRVPVAPPE"
     gene            complement(3049..3915)
                     /locus_tag="VF954_01230"
     CDS             complement(3049..3915)
                     /locus_tag="VF954_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX9529729.1"
                     /translation="MLDYHVHLWPHGERASAATVDQLAACCERAAAAGVTEIAVTEHL
                     FRFEQADAALGGFWEDDPDEDLRPVMARYWEQHVSADLDQYVDTVVGAKAAGLPVVLG
                     MEVDYYPGRMDKVGALLSGYPFDVLLGSVHWIGAWLFDYLEDPASQARWGRRGVDAVW
                     DAYTGALEELAASRTCDVLAHPDLAKVAGHRPRVPGEFHARIVEAALGSGMAAEVSSA
                     GWRKPAAEAYPAPSLLAGFHAAGVAVTTASDAHELATVADRSAELRAMVAAAGYDRLA
                     AFTGRLQRDVPL"
     gene            3999..4640
                     /locus_tag="VF954_01235"
     CDS             3999..4640
                     /locus_tag="VF954_01235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9529730.1"
                     /translation="MITDEAVERYAEAHTTPPPPHLVRADEDTRATLRAPGMMVGPLE
                     GRFLELLVYARGAARVLEIGTFSGYSALAMAAGLAAGGRITTCEVDPVHAEAAQRHID
                     DSPYADRIEIKLGPALSTIGELEGPFDVVFIDADKTNYAGYLDAVLPKLADRGVVAVD
                     NTLWSGRVVADGAGGDDADTAALRAFNERVRADPALVAVMLPVRDGVTLIRKA"
     gene            4662..5429
                     /locus_tag="VF954_01240"
     CDS             4662..5429
                     /locus_tag="VF954_01240"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX9529731.1"
                     /translation="MPDDDRPPIERILVVTAHPDDVDFGAAGTIATWTDAGIQVVYCV
                     ITDGDAGGFDPSVPRSDIPAIRRAEQEAAAKTVGVGEIHFLGYPDGRLTVSFELRRDI
                     SRIIRLVRPQRVVCQSPERNWDRLGTSHPDHLAAGEATLFAVYPDARNPFAYPELAEE
                     GFEAHAVPEIWVMGGPGQAPMSTSPARSTARSRPCAAIAASSRTPTPWTSGCAPGSGE
                     RPSRADCRKPPGQRRSAPCGWADAGWDPATEVTARRR"
     gene            complement(5410..>6428)
                     /locus_tag="VF954_01245"
     CDS             complement(5410..>6428)
                     /locus_tag="VF954_01245"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX9529732.1"
                     /translation="AVAPNTLPANAAARRSPSLPPPRPAPLPTPIPEPNRSAPARAGP
                     PPPAPGALWAGDFADPFVLLAGTTYYAFATQAGLTDVQTIKSVDLVHWQWVGDALPKL
                     PSWSSFGSVWGPAVLARPPSYVLYYATRQAATGRQCISRAVSVLPQGPYIDTSDGPVV
                     CQLDRGGSIDPSPFVGPDGTAWLQWKSEGTLQGEPTRIWSQQLSADGQQVVGQPVELL
                     HTDQAWELPIVEGPSMAQVGGLYYLLFSAGRWETSSYAIGYAVCAGPAGPCHEPTAGP
                     VLASSGAMAGPGGPDAFRDLTGSWHLAFHAWTAPDVGYPAGARSFRIAPLTMDGGKVT
                     VGGP"
BASE COUNT          914 a         2351 c         2308 g          855 t
ORIGIN      
        1 cacgtgggca tcggcttcga cgccgccgtc gtggaccagg tcgagcgccg atcgcacctg
       61 aagcgctacg ccggccaccc cctgttcgtg tacgccgcgt tcgccacctg gttccgccac
      121 tacgaccgca gccggccgca cctgacgctg gcgctggagg acccgggggc tctcggcgac
      181 ggtggccggg cggtgcccgg ctacttcgcc atctgcctca actcgacccc gtacacgttc
      241 ctcgggagcc gacggctcaa cctggcgccc gacgccgcgt tcgaccaggg gctggtggtc
      301 ctcaacttcc ggaccattcg cttctggccc gtcctcggcg tgctcgcctc ggccctcggg
      361 tcgggcgacc gcatccgcca ccaccgccgc gtcgactacc accgcgacgt ggggcgcgcc
      421 gtggtgaccg cctcgggcga accgctgccc taccaggtcg atggcgacta cgtcggcgag
      481 gtcgaccggc tggtcgtcga gcacgagccc gaggccctcc gcctcgtcat gccgtagccg
      541 agccccaact gtagccgtgc ccagccgtgc ccgtgccaag ccggccgggc caggccggcc
      601 gggccaggcc gtgctacttc ccacgcggtc atctcgtggc gttttcctat tcttccatgc
      661 ctttatgtcg ctctggcgtg tttttggctt taagaatgcc tgccccgcgt ctggccgtac
      721 tggcactcgc ccaatcggcc gacttcggtg gcgaccgatc ggccgggatg aagccgggcg
      781 cggccggcag gtgctccgga cgtcgagcgg gaggccgtcg gcctccagcc gccgggctag
      841 gagaaacgcc tcaatggtgt tttccgggca ccggttgtcg gcgccaccgg gcgaggaaag
      901 aaatcacggt ttcgggcata cgaattccgg gtaaccaagc ggttgcaact tggacgtcgg
      961 cgttttacat tggcaggaca ccccaccacc tgttcaccct gcttgcgggt ggtaccttgt
     1021 gaacgtagtc acattcacga actaggatta gcttgaagcc tcaagctcgc cgaggaggcg
     1081 ccccgtggcc ttgacctgga gccgttcgat cgactgggac acagacgatt ggcgtacccg
     1141 ggcggcgtgc cgggacacag atccggacct gttcttcccc gtggggacca ccgggcccgc
     1201 catcgagcag atcgacgcag ccagggccgt gtgccgggaa tgcgacgccc acgcccagtg
     1261 cctcgagttc gcgctggcca ccaaccagga gtccggcatc tggggcggga cgtccgagga
     1321 ggagcggcgg gccctgcgca aggcgtggct cgcccgccag cgccgcacgg cctgaaccgc
     1381 accgccagcg ccgcaccgcc tgagccgcac cgcctgagcg cccgcccaag gcccgatacg
     1441 ggcctccgcg gatcccccct gctcattccg gcggcgccac cggcacccgg agctcgacga
     1501 gcgccccgcg ggcgttcacc atggccagct cgccccccag ctggctgcgg accatctcgc
     1561 gcacgatcga cagcccgatg ctcgcggtgc gctccaggct gaagccgtca ggcaggccgc
     1621 ggccgttgtc gcggacctga acgaccaggt ccccgccctc gcggtcgacc cggacgtcga
     1681 tccggttgtc cccgccgtcg ggctcggcgg ccccgtcgtc gtcgttgaag gcatgctcga
     1741 ccgcgttctg gagcagctcg ttgagcacca ccgcgagggg ggtgaccacc tgagccggca
     1801 actcccccgc gtccccgacc gtcgagaagg tcacgacctt gccgagcgag cgggccgtgt
     1861 cctcggccat gcgcacgagc gaccggagga tctccgccga cgggacctgc tcggtgacct
     1921 cccgggacag gatctcgtgg acgaccgcga tcgagcggat ctcccgctcg acgtcctcca
     1981 gcgcatcctt cccgggcccg ggctcgagcc gccggccccg caggcggagg aggctggaga
     2041 tggtctgaag gttgttcttg acgcggtggt ggacctccct gatggtcgcg tccttggaca
     2101 cgagcaagcg gtcccgccgg cgcagggcgg tgacgtcctg gagcaggacg agggcgccgc
     2161 tgaccacccc gttgtggagc aggggcaggc actggaccag cacggtgacc tcggcccggc
     2221 tctcgatctc ctctgtgacc gcgaccgcgg tgagaaaggc ccgggccacg gccgtctcgc
     2281 cgacccccag ctcctggagg gtggcccctt cggcgttgcc gtggatgccc acccggtgca
     2341 gggcgctgac cgcgttcggc gaggtgtact cgatgcggcc gcccgcatcc aggaccagca
     2401 cgccgtcgcc cacccggggc gcctcgctga tctccgcctc gtccccgacg aaggggaact
     2461 cgccccgcac gatcatctgg gcgaaccggt cgaatatctc gacgtagacc cgctcgagct
     2521 cgccggtccg gcggccgacc gtgaggggcg cctcccgggt cagcacggcg agcagccggc
     2581 cgttccagcg aacggggatg cactgcatcc gggcccgctc cgtccgcccg gccgtggtgg
     2641 tctcgccctc gaccagctgg ccgaggtgcc acgcccgggc gaccagcggg cgctcggcct
     2701 cgtcgatgag gttcccgacg aggtcctggc ggtggagggt ctggcccgtg gtcggccgca
     2761 tctgtcccag caccacgaac tggccggaac cctggccctg ggcgggcacg aagagcaaca
     2821 ggtcggcgaa gcagaggtcg gcgagcatcc cccaggaggc catgagccgc tcgaggtgag
     2881 cgagctcggg ctccgacagg tccgtgtagg tccgacccag ctcggacggg gtggtcgcca
     2941 ccgtggcggc gtcctggggc acgagccgcg cctcaagcgc ggtcccccgt gacggggaaa
     3001 tcgggaccgc gagggcgggt gggtccgcga ccggatccac gggccggctc acaggggcac
     3061 gtcccgctgc aggcgaccgg tgaaggcggc caggcggtcg tagcccgcgg cggccaccat
     3121 cgcccgcagt tcggccgacc ggtcggcgac ggtggccagc tcgtgggcat ccgacgcggt
     3181 cgtcacggcg acaccggcgg catggaagcc ggccagcagg ctgggcgccg ggtaggcctc
     3241 cgcggcgggc ttgcgccacc cggcggagga gacctcggcc gccatccccg agccgagcgc
     3301 ggcctccacg atccgggcgt ggaactcccc cgggacgcgg ggccggtgcc ccgcgacctt
     3361 ggccaggtcc gggtgggcga gcacgtcgca ggtgcggctg gccgccagct cctccagcgc
     3421 cccggtgtag gcgtcccaca ccgcgtccac gccccggcgg ccccaccggg cctgcgacgc
     3481 cgggtcctcg aggtagtcga agagccacgc cccgatccag tgcaccgagc cgagcaggac
     3541 gtcgaagggg taacccgaca ggagcgcgcc gaccttgtcc atcctgcccg ggtagtagtc
     3601 gacctccatg ccgagcacga ccggcaggcc ggccgcctta gcccccacca ccgtgtcgac
     3661 gtactggtcg agatcggcag acacgtgctg ctcccagtag cgggccatca cgggccggag
     3721 gtcctcgtcg gggtcgtcct cccagaagcc gcccagcgcg gcgtcggcct gctcgaagcg
     3781 gaacagatgc tcggtaacgg cgatctccgt cacacccgcg gccgcagccc gctcgcagca
     3841 cgcggccagc tggtcgacgg tcgccgccga ggcccgctcg ccgtgcggcc acaggtgcac
     3901 gtggtagtcc agcacggcag ccatggtacg ggccggccca agagcggagg cgggccgaca
     3961 tcgggttgtg gtgtgatggc gcgtcgagag gagcgccgat gatcaccgac gaagccgtcg
     4021 aacgctacgc cgaggcccac accacccccc cgccaccaca cctcgtccgc gccgacgagg
     4081 acacccgggc cacgctgcgt gcgccgggca tgatggtggg accgctggag ggccggttcc
     4141 tcgagctgct cgtgtacgcc cggggagccg ccagggtgct ggagatcggg acgttcagcg
     4201 ggtactcggc gctggccatg gccgcgggcc tggcggccgg cgggcggatc accacctgcg
     4261 aggtcgaccc ggtgcacgcc gaggccgccc agcgccacat cgacgacagc ccgtacgcgg
     4321 accgcatcga gatcaagctc ggccccgcgc tgtcgacgat cggcgagctg gaggggccgt
     4381 tcgacgtggt gttcatcgac gccgacaaga ccaactacgc cggctacctc gacgccgtcc
     4441 tgcccaagct cgccgaccgc ggcgtggtcg cggtcgacaa cacgctctgg agcggccggg
     4501 tcgtggcgga cggcgcaggc ggcgacgatg cggacacggc ggcgttgcga gcgttcaacg
     4561 agcgggtgcg cgccgacccg gcgctcgtgg ccgtgatgct cccggttcgc gacggggtca
     4621 cgctcattcg caaggcgtag cggccggtag ggtcaccggg catgccggat gacgaccgcc
     4681 caccgatcga acgcatcctg gtcgtgacgg cgcatcccga cgacgtcgac ttcggggcgg
     4741 cgggaactat cgccacctgg accgacgccg gcatccaggt ggtctactgc gtcatcacgg
     4801 acggcgacgc aggcggcttc gacccgagcg tcccccgctc cgacataccc gccatccggc
     4861 gggcggagca ggaggccgcg gccaagacgg tcggcgtcgg ggagatccac ttcctcggct
     4921 atccggacgg gaggctgacg gtgagcttcg agctgcggcg cgacatcagc cggatcatcc
     4981 ggctggtgcg gccccagcgg gtggtgtgcc agtcgccgga gcgcaactgg gaccgtctcg
     5041 gcaccagcca cccggaccac ctggccgccg gcgaggccac cctgttcgcg gtgtacccgg
     5101 acgcccgcaa tcccttcgcc tacccggagc tcgccgagga gggcttcgag gcccatgccg
     5161 tgccggagat ctgggtgatg gggggcccgg ggcaggcacc tatgtcgaca tcaccggcac
     5221 gctcgaccgc aaggtcgcgg ccctgcgctg ccatcgcagc cagctcccgg accccgacgc
     5281 catggacgag cgggtgcgca cctgggtccg gcgaacggcc gagcagggcg gactgccgga
     5341 agccgccggg gcagaggcgt tccgcgccgt gtgggtgggc tgacgcggga tgggacccgg
     5401 ccacggaggt cacggcccgc cgacggtgac ctttccccca tccatggtca gcggcgcgat
     5461 ccggaagctg cgtgcgcccg cggggtaacc cacgtccggc gccgtccagg cgtggaaggc
     5521 gaggtgccag ctcccggtca ggtccctgaa ggcgtcgggc ccacccgggc ccgccatggc
     5581 tcccgacgag gccagcaccg ggccggcggt gggctcgtgg cagggaccgg ccggaccggc
     5641 gcacaccgcg tagccgatgg cataggacga cgtctcccac cggccggccg agaagagcag
     5701 gtagtacaac cctccgacct gcgccatcga cgggccctcc acgatcggga gctcccacgc
     5761 ctggtcggtg tgcaacagct ccactggctg gcccaccacc tgctggccgt cggcgctgag
     5821 ctgctgcgac cagattcggg tcggctcgcc ctggagcgtc ccctcgctct tccactgcag
     5881 ccaggccgtc ccgtcgggcc cgacgaaggg gctggggtcg atggacccgc cgcggtcgag
     5941 ctggcacacc acggggccgt cggaggtgtc gatgtagggc ccctgcggca gaacgctgac
     6001 ggcccgggag atgcattgcc ggccggtcgc cgcctgccgg gtggcgtagt acagcacgta
     6061 ggacggcggc cgggccagca ccgccggccc ccacaccgat ccgaagctcg accacgacgg
     6121 gagcttcggc agggcgtcgc cgacccactg ccagtgcacg aggtcgaccg acttgatggt
     6181 ctggacgtcg gtgagccccg cctgggtggc gaaggcgtag tacgtcgtcc ccgccagcag
     6241 cacgaacggg tcagcgaagt ccccggccca cagcgcgccc ggcgccggcg gtggcggccc
     6301 ggcgcgggcg ggagcgctgc ggttgggctc ggggatcggc gtcggcagcg gggcggggcg
     6361 cggcggcggc aaggacggcg agcggcgggc cgccgcgttg gccgggaggg tgttgggggc
     6421 gacggcgg
//
LOCUS       DASWGG010000073         5319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1967517, whole genome shotgun sequence.
ACCESSION   DASWGG010000073 DASWGG010000000
VERSION     DASWGG010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967517"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VF954_01250"
     CDS             <1..1258
                     /locus_tag="VF954_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX9529733.1"
                     /translation="RAAGDRPVSGPDAEHAAGDPTGDPAYRLAEATVNETAGIRVHGT
                     IRTRVRGSLRELAGARGVTPLALARDLGADAPAVEALVEAATVQESWWFRDPAQMAVV
                     ADLEAQWGAVGHLWCAGCAHGQDPYSLAIALDEVGLTRWRVLATDIDQAALARTRTAA
                     YSGRELRGLSPAHRERFFEPDGLQWTVRPMIRRRVAVLHHNLATEPSPLGARWPVIFC
                     RNVLMYFDAEHRSRTAQLLVDALAPGGWLFLGSAESLWRVHDDLELVRMGAGLAHRRR
                     VRPPRAIGGSAPAVAAEPRRATAGRPDAWGDGEGVERELAALRSAGLAAFRGGDLRRA
                     VRDLRRAAYLSASDAVDHFHLGVALERAGHPVAARRAFRGALATSDTTDRDRIDAALD
                     EHREEHPEEPLAVGFDQLLRGKVNGR"
     gene            1255..2343
                     /locus_tag="VF954_01255"
     CDS             1255..2343
                     /locus_tag="VF954_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX9529734.1"
                     /translation="MSSTVLVVDDSDTVRMSLRHHLEGAGYQVREAAHGQDALALARS
                     AGPDVVLLDLEMPGIDGREILAVFQREEMLASIPVVVVSSHRRVHEVVESMEAGAHDF
                     LAKPFEPAELLARVRAAARVKALNDELRRRNHELELFAQRASHDLKSPLSVMKGMAET
                     LVAQGDALDPALRVRLLERISAAADRASQMVTDLLALALNGTPSTVATPGTDLQPVVE
                     ELTGPELLFDAVVEISGELGMVAAPRPDVVGVLFNLIDNASHYGRSADGVLRLHVAGE
                     GRDGVQTVSIRDRGAGIPEPDRANLFQPFHRGESSLVVNPTSTGIGLAIVARATARWG
                     GSATFETGSEGGACFVIRLPLAPGDDQS"
     gene            complement(2366..2851)
                     /locus_tag="VF954_01260"
     CDS             complement(2366..2851)
                     /locus_tag="VF954_01260"
                     /inference="COORDINATES: protein motif:HMM:NF022219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial proteasome activator family protein"
                     /protein_id="HEX9529735.1"
                     /translation="MSDDPREQVPSLIVVPGATAPGDDQRGQPETVELPAKVMRIGSM
                     VKQLLDEVRQAPLDDAGRMRLREIYETSVRELASGLSPDLAAELDRVTMPFGEGIPSG
                     AELRVAQAQLVGWLEGLFHGIQATLFAQQMQARSQLEDMRRRGLPPGPDDGDPRPGTY
                     L"
     gene            complement(2941..4050)
                     /locus_tag="VF954_01265"
     CDS             complement(2941..4050)
                     /locus_tag="VF954_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9529736.1"
                     /translation="MSAPSPVRPDYAGACLTNLTAAVRAVPAKRPAWLPAPAREAERV
                     VLLLLDGLGWQQLQGAPSLAPTMVGMVGGRATSVAPTTTATALTSLTTGVAPAAHGVV
                     GYRVRVPGDEVMNVLAWRTPAGDARQRVDPASFVRAAAFPGGPVVPVVTRSEFAATGF
                     TVAHLGGSRLIGWRQASTLVVEVDHLLKAGVPYVYAYYDGIDRVAHEYGLGEHWEAEV
                     RASDRLVSDLIEILPPDAALVVTADHGQVDVGEGARAVPAGVGAATAFMSGEGRFRWL
                     HARPGAGADLLAAATESFGDEAWVFSRDELVGDGWLGGEPDGDVCLRLGDVAIVPFAP
                     IAIIDPADPGERRLMSRHGSLTEAEMYVPLLAQGG"
     gene            4135..4584
                     /locus_tag="VF954_01270"
     CDS             4135..4584
                     /locus_tag="VF954_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9529737.1"
                     /translation="MKVSTRGDYASRALLSLALHTDDQGPTSVRDIAERTALPQPYLE
                     QILLALKGAGLVRSKRGVGGGYVLARPAAEILLGQIVAAVDGPIVAGDFGNPHENGAC
                     DHEGQCVLLSVWSEVGEHMRSHLDSFTLADMVARAQGTAAPTLAPLL"
     gene            complement(4590..>5319)
                     /locus_tag="VF954_01275"
     CDS             complement(4590..>5319)
                     /locus_tag="VF954_01275"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX9529738.1"
                     /translation="ADAGAQGDPTVSVPVVYADAEVIVVDKPAGMVVHPGAGHAAATM
                     VQGLLARFPDLAAVGDPTRPGIVHRLDKGTSGLLVVARTPRALASLVGQLKSRSAGRE
                     YDTLVWGRVEAPTGMIDAPVGRSGRDPTRMAVTARGREARTRYAVIGRYATATATAVT
                     RLACNLETGRTHQVRVHLAAIGHPVVGDARYGGRRPELRTGRPFLHARGLAFVHPVTG
                     EAMAFSSPLPDDLAATLDHLRPEP"
BASE COUNT          731 a         1975 c         1912 g          701 t
ORIGIN      
        1 ccgagcggct ggggaccgcc ccgtgagcgg gcccgacgcc gagcacgcgg ccggcgaccc
       61 gaccggcgac cccgcctacc ggctcgcgga ggccaccgtc aacgagaccg cgggcatccg
      121 cgtccacggg accatcagga ctcgcgtccg cgggagcctg cgggagctgg caggggcccg
      181 gggggtcacc ccgcttgcgc tcgcccgcga tctgggagcc gacgcgccag cggtcgaggc
      241 gctcgtcgag gccgcgacgg tgcaggagag ctggtggttc cgagacccgg cgcagatggc
      301 cgtggtagcc gacctcgaag cccagtgggg cgccgtcggc cacctctggt gcgcgggctg
      361 cgcccacgga caggatcctt acagcctcgc catcgccctc gacgaggtcg gcttgacccg
      421 ctggcgggtc ctggcgaccg acatcgacca ggccgccctg gcccgcaccc gcaccgcggc
      481 ctactccggc cgggagctcc gggggctctc gcccgcccac cgggagcgct tcttcgaacc
      541 ggacggcttg cagtggaccg tgcgccccat gatccggcgg cgcgtagccg tcctgcacca
      601 caacctggcc accgagccct ccccgctcgg agcgcggtgg ccggtgatct tctgccgcaa
      661 cgtgttgatg tacttcgatg ccgagcaccg cagccggacg gcccagctgc tggtcgacgc
      721 gctggcgccc ggcgggtggc tgttcctggg gtcggccgag tccctctggc gggtccacga
      781 cgacctcgag ctcgtgcgga tgggcgctgg cctggcccac cggcgccggg tgcggcctcc
      841 cagggcgatc ggcggttctg ccccggcagt cgcagccgaa ccacggcggg ccaccgccgg
      901 acggcccgac gcgtggggtg acggcgaagg ggtcgagcgc gagctggccg ccctccgctc
      961 ggcgggcctg gccgccttcc ggggcgggga ccttcgcaga gcggtacgag acctgcgtcg
     1021 agccgcctat ctttctgcgt ctgacgccgt tgaccacttc cacctcgggg tggcgctgga
     1081 gcgggcgggc caccctgtcg ccgcccgtcg ggccttccgg ggcgcgctgg ccacctcgga
     1141 cacgactgat cgggaccgga tcgatgccgc gctggacgaa catcgggagg agcatccgga
     1201 ggagcctttg gccgttggct tcgaccagct gctacggggc aaggtgaacg gccgttgagc
     1261 tcgaccgttc tcgtcgtgga cgactccgac accgtgcgga tgtcgctgcg ccaccacctc
     1321 gaaggcgccg gctaccaggt gcgcgaggcc gcgcacggtc aagacgctct ggccctggcc
     1381 cggtccgccg gacccgacgt cgtcctgctc gatctcgaga tgccgggcat cgacgggcgg
     1441 gagatcctcg cagtgttcca acgagaggag atgctggcgt cgatccccgt ggtcgtcgtg
     1501 agcagccacc ggcgggtcca cgaggtcgtc gagtccatgg aggctggggc gcacgacttc
     1561 ctggccaagc cgttcgagcc ggccgagctg ttggcccggg tccgggccgc ggcccgggtg
     1621 aaggccctca acgacgagct gcgccgccgc aaccacgagc tggagttgtt cgcccagcgg
     1681 gcctcccacg acctgaaatc gccgttgagc gtgatgaagg gcatggccga gacgctggtg
     1741 gcgcagggcg acgccctcga cccagccctg cgcgtccgcc tgctcgagcg catctcggcg
     1801 gccgccgacc gcgcctctca aatggtcacc gatctcctgg cgctggctct gaacggaaca
     1861 ccgtccaccg tcgccacgcc gggcaccgac ctccagccgg tggtggagga gctgaccggt
     1921 cccgagctct tgttcgacgc cgtggtggag atctccggcg agctcggcat ggtggcggcg
     1981 ccccggcccg acgtggtggg ggtgctgttc aacctgatcg acaacgcctc gcactacggc
     2041 cgcagcgccg atggagtcct ccgcctgcac gttgccggcg aaggccgcga cggggtgcag
     2101 accgtctcga tccgcgacag gggggcgggc atccccgagc cggatcgtgc caacctgttc
     2161 cagccgttcc accggggcga gtcgtccctc gtcgtcaacc ccacctcgac cggcatcggt
     2221 ctggcgatcg tggcccgagc cacggcgcga tgggggggct cggccacctt cgagacgggc
     2281 tccgaaggcg gcgcctgttt cgtcatccga ctacccctgg cgcccggcga cgatcagagc
     2341 tgaggcccgg ccgggccccg agcggtcaga ggtacgtacc cggccgcggg tcgccgtcgt
     2401 ccgggccggg cggtagccca cgccggcgca tgtcctccag ctgggaccgg gcttgcatct
     2461 gctgggcgaa cagcgtggcc tggatgccgt ggaaaaggcc ctcgagccag cccaccagct
     2521 gggcttgggc gacgcgcagc tcggccccgg acgggatccc ctccccgaag ggcatggtca
     2581 cccggtctag ctcggcggcc agatcgggcg agagccccga cgccagctcg cgcaccgacg
     2641 tctcgtagat ctcccggagg cgcatgcgcc ccgcatcgtc gagcggcgcc tgacgcacct
     2701 cgtcgaggag ctgcttgacc atcgagccga tcctcatcac cttggccggc agctcgacag
     2761 tctctggttg cccccgctgg tcgtcgcctg gtgcggtcgc gccaggaacc acgatcagcg
     2821 agggaacctg ctctcggggg tcatcggaca tgagcccatc cttccccggc gagtcggccg
     2881 gcaccaaatg gtcaccaaat ggtatggctg ccggcgccgc gccggccgac cctggacccc
     2941 tcatcccccc tgcgccagca gcgggacgta catctcggct tcggtgagcg acccgtggcg
     3001 gctcatcaac cggcgctccc cagggtcggc gggatcgatt atggcgatgg gcgcgaacgg
     3061 gacgatcgcc acgtcgccga gccgcaggca cacgtcgcca tcgggctctc cccctagcca
     3121 gccgtcgccc accagctcgt cccggctgaa cacccaggcc tcatccccga acgactccgt
     3181 ggccgccgcc agcaggtcgg cccccgcacc cggccgggcg tgcaaccacc ggaaccggcc
     3241 ctcaccggac atgaacgccg tggcggcgcc aactccggcg ggcaccgccc gcgcaccctc
     3301 gcccacatcg acctgcccgt ggtcagccgt gaccacgaga gccgcgtcgg ggggcaggat
     3361 ctcgatcaga tcgctcacca gccgatcgga ggcccggacc tccgcctccc agtgctcgcc
     3421 gaggccatac tcgtgcgcta cccggtcgat gccgtcatag taggcgtaca cgtacgggac
     3481 gccggccttg agcaggtggt ccacctccac gaccaacgtg gaagcctgcc gccagccgat
     3541 cagccggctc ccgcccagat gggccaccgt gaagccggtc gccgcgaact ccgaccgggt
     3601 cacgaccggg acgaccggtc cacccgggaa cgccgcggcg cgcacgaacg atgccgggtc
     3661 gacgcgttgg cgggcgtcgc ccgcgggtgt gcgccaggcc agcacgttca tcacctcgtc
     3721 gcccgggacc cggacgcggt aaccgacgac accgtgggcc gccggcgcca cacccgtcgt
     3781 caacgacgtg agggcagtcg ccgtcgtggt cggcgcgaca gaggtggctc gcccaccgac
     3841 catgccgacc atcgtcggcg ccagcgacgg agcaccctgg agctgctgcc agcccaggcc
     3901 gtcgaggagc agcaggacga cgcgctcggc ctcccgggcc ggggccggga gccaggcggg
     3961 ccgcttggcg gggacagcac gtacggctgc ggtgagattg gtgaggcacg cccccgcgta
     4021 gtcgggccgg acgggcgagg gcgcgctcac gggccgaagc taatgactcc ggccgggcag
     4081 cggcgggatg cgccgcaaag gcccaggagc cggcccaggc cgtagcatgg gcacgtgaag
     4141 gtctcgacga ggggtgacta cgccagccgg gcgttgctct ccctcgccct gcacaccgac
     4201 gaccagggac ccacgtcggt gcgtgacatc gcggagcgta ccgcgctgcc ccagccctac
     4261 ctcgagcaga tcctcctcgc cttgaagggt gccggcctgg tgcggtccaa acgcggcgtc
     4321 ggcggcggct acgtgctggc ccggccggcc gccgagatct tgctgggcca gatcgtggcc
     4381 gccgtcgacg gcccgatcgt cgccggcgac ttcgggaacc cgcacgagaa cggagcctgc
     4441 gaccacgagg gccaatgcgt gctgctgtcc gtctggtccg aggtgggcga gcacatgcgc
     4501 tcgcatctcg actccttcac cctcgccgac atggtggccc gggcccaggg cacggctgcg
     4561 ccgacgctgg cgcccctgct ctgagccggt cagggctcgg gccggaggtg gtccagggtg
     4621 gcggccagat cgtcgggcag cggggacgag aaggccatcg cctctcccgt cacggggtgc
     4681 acgaacgcca ggccccgagc atgtagaaag ggccggccgg tgcgcagctc ggggcgccgg
     4741 ccgccgtacc gggcgtcgcc cacgacggga tggccgatgg cggccaggtg cacccggacc
     4801 tggtgggtgc gcccggtctc caggttgcag gccaggcggg tcacggcggt ggcggtggcg
     4861 gtggcgtagc ggccgatgac cgcgtacctg gtgcgcgcct cgcggccgcg ggccgtgacc
     4921 gccatgcgcg tcgggtccct cccgctgcgg cccaccggcg cgtcgatcat ccccgtcggt
     4981 gcctcgaccc ggccccagac cagggtgtcg tactcccggc cggcgctgcg ggacttgagc
     5041 tggccgacga ggctggcgag cgcacgtggc gtgcgggcca cgacgagcag cccggacgtg
     5101 cccttgtcga gccggtggac gatccccggg cgggtggggt cgcccaccgc ggcgaggtcc
     5161 gggaagcggg cgagaagccc ctggaccatg gtcgcggcgg cgtggccagc acccggatgc
     5221 acgaccatcc cggccggctt gtcgaccacg atcacctcgg cgtccgcgta cacgacgggc
     5281 accgacacgg tcgggtcgcc ctgcgcgccg gcgtcggcc
//
LOCUS       DASWGG010000074         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2650355, whole genome shotgun sequence.
ACCESSION   DASWGG010000074 DASWGG010000000
VERSION     DASWGG010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2650355"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VF954_01280"
     CDS             complement(<1..723)
                     /locus_tag="VF954_01280"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX9529739.1"
                     /translation="MDPIGLKAVELDALAELATRLATDAGALVLRHQPETRTDVQTKS
                     SATDMVTEVDRASESLIVSRIRSARPGDAIEGEEGTADGGISGVTWLVDPLDGTTNYL
                     YDYPAYSVSVAVLSGGVAAVGVVFDPVRGELFRAVAGRGATRSGVPLHLSSGPALDSA
                     LVGTGFSYEAARRAHQAFALTAVLPRVRDIRRGGSAALDLCWVAAGRLDAFYERGLQP
                     WDRAAGALVAAEAGAWVGAGRRP"
     gene            767..>1531
                     /locus_tag="VF954_01285"
     CDS             767..>1531
                     /locus_tag="VF954_01285"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HEX9529740.1"
                     /translation="MVVPVDLRSDTVTLPTQAMRRAMAEAEVGDDVYGEDPMVNALQE
                     AFAERVGKPAAIYVPSGTMGNQLALRLLAPPGSWVVAGARQHVVVYEGGAAGRNAGVQ
                     FHTVPDPEGVFSAADVGWAIQAASHHHPRPGLVCVENTHMPADGAPWPLEVLDAVAEV
                     AAGAGLPVHMDGARLFNAEVATGTAASAYAARATTVMCCLSKGLCAPVGSLLAGPHDV
                     IEEARSERARLGGGMRQAGVIAAAGLVALRTMVDRLA"
BASE COUNT          190 a          567 c          589 g          185 t
ORIGIN      
        1 cggccgccgg ccggccccga cccatgcgcc cgcctcggcc gcgacgagcg cgccggccgc
       61 ccggtcccac ggctgcaggc cccgctcgta gaaggcgtcg agcctcccgg ctgcgaccca
      121 gcacagatcg agggcggccg agccgccccg gcggatgtcg cggacccggg gaagcaccgc
      181 cgtcagggcg aaggcctgat gggcgcgccg ggcggcctcg taggagaagc cggtgccgac
      241 cagggcggag tccaacgccg ggccggacga gaggtgcagg gggacgccgc tgcgtgtggc
      301 gccccggccg gcgacggccc ggaacagctc gccccggacc gggtcgaaca ccaccccgac
      361 cgccgccacg ccgcccgaca ggacagccac cgagaccgag taggccgggt agtcgtacag
      421 gtagttggtc gtcccgtcga gcgggtcgac gagccacgtg accccgctga tgccgccgtc
      481 cgccgtcccc tcctcgccct cgatggcgtc gccgggccgg gcggagcgga tccgcgacac
      541 gattagcgac tcgctggccc ggtccacctc ggtgaccatg tccgtggccg acgacttggt
      601 ctgcacgtcc gtgcgggtct cgggctggtg gcggagcacg agcgcgcccg cgtcggtggc
      661 cagccgggtg gcgagctcgg ccagggcgtc cagctcgacg gccttcagcc cgatggggtc
      721 cacggcgcgt gtctaggcga aatctcgccg gtagcgtggc ccacccatgg tcgtcccggt
      781 ggacctgcgc tcggacacgg tcaccttgcc gacccaggcg atgcgccggg cgatggccga
      841 ggccgaggtg ggcgacgacg tctacggcga ggaccctatg gtgaacgccc tccaggaggc
      901 cttcgccgag cgggtcggca agccggcggc catctatgtc cccagcggga ccatgggcaa
      961 ccagctcgcc ctccggctgc tggccccgcc gggcagctgg gtggtggccg gcgcccggca
     1021 gcacgtggtc gtctacgagg gcggggcggc cgggcgcaac gccggcgtgc agttccacac
     1081 ggttcccgat cccgaagggg tcttctcggc cgcggacgtc ggctgggcga tccaagcggc
     1141 gtcgcaccac cacccccgcc cggggctggt gtgtgtcgag aacacccaca tgccggccga
     1201 cggcgctccc tggccgctcg aggtcctcga cgcggtggcc gaggtcgcgg ccggcgccgg
     1261 gctgccggtg cacatggatg gggcccggtt gttcaacgcc gaggtggcga cgggcacggc
     1321 ggcgtcggcg tacgccgccc gggcgaccac ggtgatgtgc tgcttgtcca aggggctgtg
     1381 cgcgccggtc ggctcgctgc tcgccgggcc gcacgacgtc atcgaggagg cccgatcgga
     1441 gcgcgcccgc ctcggcggcg ggatgcggca ggccggcgtg atcgccgcgg ccgggctggt
     1501 cgccctgcgg accatggtgg accgcctcgc c
//
LOCUS       DASWGG010000075         4348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1040120, whole genome shotgun sequence.
ACCESSION   DASWGG010000075 DASWGG010000000
VERSION     DASWGG010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040120"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VF954_01290"
     CDS             <1..586
                     /locus_tag="VF954_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529741.1"
                     /translation="PMEERHIYPRDYIEDSLAVRMGGRVAEMLTYGDLSTGANNDLVG
                     NTELARKMVREWGMSDRIGPMAWGSQGMVFLGEDLMHSRDYSEDTSRVIDEEVERILR
                     EAEQRAHEYLTAHRKGLAAVAVALLEHETIDGAEVGRLVDRAFGGPVSKSRGSVIPHF
                     ATTEPTDAGPNGRPARARSRSRAGADDTKAIKPN"
     gene            complement(583..1002)
                     /locus_tag="VF954_01295"
     CDS             complement(583..1002)
                     /locus_tag="VF954_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529742.1"
                     /translation="MLVRLAVALALVVVAVAVARLVQRRRPDPPTQPRWAVPTQLDRA
                     DFSCPAAPWLLVVFTSAACESCARATLKASVLASPEVAYQEVSWQDRADLHERYHVEA
                     APTIVLADHEGVVRASFVGVPEATDLWAAVAEARQPG"
     gene            1048..2439
                     /locus_tag="VF954_01300"
     CDS             1048..2439
                     /locus_tag="VF954_01300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9529743.1"
                     /translation="MSESKVGAGPMWVLGLVIMIDQVDQNILRGIETPLRADRALHVN
                     DFKFGLLLASFVLVNGLLSVPAGYLADRGNRTRTVGHTIVAWSGISALTAASPNFGVL
                     LGVRSALGFGQAMAEPSCASLLADYYPAEQRGLAFSIQQCLVFAGIGLGVGVGGFVGN
                     TFGWRWAFLLVGTPGVLIALAMYRMREPRRGEGDRLHLGIAEAGTADADLRPALFDDG
                     FGAFVRDMGKGLAADMRTIWGIPTMRYALVGVSSLLFSVTALAAALPQFYERNLHVAK
                     AAAPGYVGVVIVVGGIPGVLLGGRLADRWAPKIRGGRMALPAYCIMGGQVFLVASYVM
                     THILPAIILEMLGAFVITLAIPALRAGTSDALPANLRGAGFGAFNLASVIFGQAAAST
                     VVFSLATAFGHDLRTALLIVSPPAFLGGLIFLRARNHLDEDAAKIFAAIMTALAAQQA
                     RDAARQAEATGEG"
     gene            complement(2470..3933)
                     /locus_tag="VF954_01305"
     CDS             complement(2470..3933)
                     /locus_tag="VF954_01305"
                     /inference="COORDINATES: protein motif:HMM:NF040160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HEX9529744.1"
                     /translation="MTPARRGFVVLLMAAALVGAGVADRSGRESSASPSSRSSLLPSV
                     LEAAAVMPSAASARALASAWSCPLATAAGAAGAAEGQVVVANVGDGPLSGVVTVTPAD
                     GEAANKTITVDPHSSLQVKESDVVHLGADRPWASALVDLHGGQAGVELLASGQWGTSI
                     TPCSPSASDHWYFATGSTAVDATMMMGLFNPFPSDAVVDLAFVTDQGPANPSEFQGVV
                     VPARGLRVLDIGDHVRRRDALAATVTARTGRVVAERLQARTGGNAGLTAALGAPSPGE
                     TWYFPDGYSADHDIEHYVIYNPEPAEAHVSLKVNLDTGSAEPFDLTVPPAATFTVMVE
                     SESRIPPQVGHSAELHSLNGVPVVVERTVQASAPAPRVGGGAMVGARVAAPRWLLPVG
                     NATAGVDEWVVVYNPGAMAATVSLRALEDGQEMAIPQMQGVAIPPGRRHAFRLGDQLQ
                     LSPLPVVVGATAPVVVERDLYVNGQPVLILNAGEPLP"
     gene            complement(4119..>4348)
                     /locus_tag="VF954_01310"
     CDS             complement(4119..>4348)
                     /locus_tag="VF954_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529745.1"
                     /translation="AYQVDTAGDGHLGFRTPPLRWLAVGAQMLLWLAVAGALLTGRRR
                     GGPERPAPEAARRGAAPAGAAADEHTTAVPA"
BASE COUNT          598 a         1592 c         1580 g          578 t
ORIGIN      
        1 gcccatggag gagcgccaca tctacccccg cgactacatc gaggactcgc tcgcggtccg
       61 catgggcggg cgggtagcgg agatgctgac ctacggcgac ctgtccacgg gcgccaacaa
      121 cgacctggtg ggcaacaccg agctggcccg caagatggtc cgcgagtggg gcatgagcga
      181 ccggatcggg cccatggcgt gggggtcgca gggcatggtg ttcctcggcg aggacctcat
      241 gcacagccgg gactacagcg aggacaccag ccgggtgatc gacgaggagg tcgagcgcat
      301 cctccgggag gccgagcagc gggcccacga gtacctgacc gcccaccgca agggcctggc
      361 cgcggtggcg gtcgccctgc tcgagcacga gaccatcgac ggggccgagg tcgggcgcct
      421 ggtcgatcgg gccttcggcg ggccggtctc caagagccgg gggtcggtga tcccccattt
      481 cgccacgacg gagccgaccg atgcggggcc caacgggcga ccggcccggg cccggtcgcg
      541 cagccgggcc ggggcggacg acaccaaggc gatcaagccc aactagcccg gctggcgggc
      601 ttcggccacc gcggcccaga ggtccgtcgc ctcgggcacg cccacgaacg acgcccgcac
      661 cacgccctcg tgatcggcga gcacgatggt gggcgcggcc tcgacgtggt agcgctcgtg
      721 cagatcggcg cggtcctgcc aggacacctc ctggtaggcc acctcagggc tggccagcac
      781 gctggccttc agggtcgccc gggcgcatga ctcgcaggcg gccgacgtga acacgaccag
      841 caaccagggc gccgccggac aggagaagtc ggcccggtcg agctgggtgg gcacggccca
      901 gcggggctgc gtcggcgggt ccggtcggcg gcgctggacc agcctggcga ccgcgacggc
      961 taccacgacg agggcgaggg ccaccgcgag gcgcacgagc acccgtccac tatgcaccgt
     1021 ggccggggaa ccgctacaaa cggcagggtg agcgagtcga aggtgggggc agggccgatg
     1081 tgggtgctcg gcctggtcat catgatcgac caggtcgacc agaacatcct ccgcggcatc
     1141 gagaccccgc tgcgagccga ccgggccctg cacgtcaacg acttcaagtt cgggctgctg
     1201 ctcgccagct tcgtgttggt gaacggcctc ctcagcgtgc cggccggcta cctggccgac
     1261 cgaggcaacc ggacgcgcac cgtcggccac accattgtgg cgtggtcagg catcagcgcc
     1321 ctcacggccg cctctcccaa cttcggggtg ctgctcggcg tccggtcggc gctgggtttc
     1381 ggccaagcca tggccgagcc gtcctgcgcc agcctgctcg ccgactacta cccagccgag
     1441 cagcgaggcc tggccttctc catccagcag tgtctggtct tcgctggcat cggcctcggc
     1501 gtcggcgtgg gagggttcgt cggcaacacc ttcggctggc gctgggcgtt cctgctcgtc
     1561 ggcacgcccg gggtgttgat cgcgctggcc atgtaccgca tgcgcgagcc gcggcggggc
     1621 gaaggcgacc gccttcacct ggggatcgcc gaggctggca cagccgacgc cgacttacgg
     1681 ccggccctgt tcgacgacgg gttcggcgcc ttcgtgcggg acatgggcaa gggactggcc
     1741 gccgacatgc gcaccatctg gggcatcccg accatgcgct acgccttggt cggtgtgtcc
     1801 tcgttgctgt tctcggtcac ggccctggcc gcggccctgc cacagttcta cgagcgcaac
     1861 ctgcacgtgg ccaaggcggc ggcccccggc tacgtgggcg tggtgatcgt cgtcggcggc
     1921 atcccgggcg tgctcctcgg cgggcggctc gccgatcggt gggcgccgaa gatcaggggc
     1981 ggccggatgg cccttcccgc ctactgcatc atgggcggcc aggtgttcct ggtggcctcc
     2041 tacgtgatga cccacatcct cccggccatc atcctcgaga tgctcggcgc cttcgtgatc
     2101 accctcgcca tcccggccct ccgcgccggg acatcggacg cactccccgc caatcttcga
     2161 ggcgccgggt tcggcgcctt caacttggcg tccgtcatct tcggtcaggc cgcggcctcg
     2221 accgtcgtgt tctcgctggc caccgcgttc ggccacgatc tgcgcaccgc cctgctgatc
     2281 gtcagcccgc cggcattcct gggtgggctg atcttcctgc gggcgcgcaa ccacctcgac
     2341 gaggacgccg ccaagatctt cgccgccatc atgacagcct tggcggcgca gcaggctcgg
     2401 gacgcggcca ggcaggccga ggctaccggc gagggctgag gctcccgcac ctctacgaca
     2461 tccggcgctc taggggaggg gctcgcccgc gttcaggatg agcaccggct ggccgttgac
     2521 gtacaggtcg cgctccacca ccaccggcgc ggtcgcgccg acgacgaccg gcaggggact
     2581 cagctggagc tgatcgccga ggcggaaggc gtggcggcgc ccgggtggta tagccacccc
     2641 ctgcatctgg ggaatcgcca tctcctggcc atcctccagg gcgcgcagcg agacggttgc
     2701 cgccatggcg ccggggttgt acacgactac ccactcgtcg acgcccgcgg tggcgttccc
     2761 gaccgggagc agccaccggg gggcggcgac ccgcgcgccc accatggcgc ccccgccgac
     2821 gcggggcgcc ggcgccgagg cctggacggt gcgctcgacg acgacgggca cgccgttgag
     2881 ggagtgcagc tctgccgagt ggccgacctg gggcgggatg cgagactcgc tctcgaccat
     2941 gaccgtgaag gtggccgcgg gcggcacggt caggtcgaag ggctcggcgg aaccggtgtc
     3001 gaggttgacc ttcagcgaga cgtgcgcctc tgccggctcc gggttgtaga tgacgtagtg
     3061 ctcgatgtcg tgatcagctg agtagccgtc tgggaagtac cacgtctccc ccggcgaagg
     3121 cgcgcccagg gccgccgtca gcccggcgtt gcccccggtc cgggcctgga ggcgctcggc
     3181 cacgacgcgg ccggtgcgag ccgtcacggt ggcggccagg gcgtcgcggc gccggacgtg
     3241 gtctccgatg tcgaggaccc gcagtcccct cgcggggacg acgaccccct ggaactcgct
     3301 cgggttcgcc ggtccctggt cggtcacgaa ggccaggtcc accaccgcgt ccgacgggaa
     3361 ggggttgaac aaacccatca tcatggtggc gtccaccgcg gtcgatccgg tcgcgaagta
     3421 ccagtggtcc gaggcgctcg gcgagcacgg cgtgatgctg gtgccccact gcccgctggc
     3481 cagcagctcc acgccggcct ggccgccgtg caggtcgacc agggcgctgg cccaggggcg
     3541 gtcggcgccc aggtgcacca cgtcggactc cttgacctgg agggacgagt gggggtccac
     3601 ggtgatggtc ttgttggcag cctcgccgtc ggccggcgtg accgtgacga cgcccgaaag
     3661 gggcccgtcg ccgacgttgg ccacgaccac ctgcccttcc gcggcgccgg ccgcccctgc
     3721 ggctgtagcg agggggcagg accacgccga cgccagggcg cgagccgacg ccgccgacgg
     3781 catcaccgcg gccgcttcca gcaccgacgg caagagcgag gaccgcgacg acggcgaggc
     3841 gctcgactcc cgccccgacc tgtcggccac accggcgccg accagcgccg ccgccatcag
     3901 gaggaccacg aagccccggc gggcgggcgt cacccccgag gacgtggccg gcctgatgcc
     3961 cccaccggag acggtccgcg ggccccgtgg gcccggggcc ggccccggcc ggtcgctcca
     4021 gccgagatcg tcgtcgtcac cgccgaaggg gccgctcgcg ggccacggcc actcgccgtc
     4081 ttcccggtct cgcccgggcc ggcgcccgtg cgccggcgtc acgcgggcac cgcggtcgtg
     4141 tgctcgtcgg ccgcagctcc ggccggagcc gcaccgcgac gggcagcctc gggggccggg
     4201 cgctcgggcc caccccgccg ccggcccgtc agcagggcgc cggcgacggc cagccagagc
     4261 agcatctggg ccccgacggc caaccaccgc agcgggggcg tccggaagcc gaggtgaccg
     4321 tcccccgcgg tgtccacctg gtaggcgc
//
LOCUS       DASWGG010000076         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_717384, whole genome shotgun sequence.
ACCESSION   DASWGG010000076 DASWGG010000000
VERSION     DASWGG010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717384"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VF954_01315"
     CDS             <1..1145
                     /locus_tag="VF954_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529746.1"
                     /translation="FFLGATDPNVQADWFIRGHGPYATTVGIAATLLVGFVLIGITQG
                     VLTGDVAGMLRRITFELPVAVLAMVGLITVTQALIRLTDELSRGLLKQFGDDVQHFTA
                     GVNSVSALGGPTTSSFVVVVLALVSVLAGVVLVAELVVRSALIYIVVALAPLVFAAQL
                     WPALRGVARKLLELLVALIVSKLVVAIALAVAAAAAVGAGAGAGGQVSSLSPPEVLAH
                     SPGGSITQAVGILLAATAAFGVAAFSPLLIARLLPLTEAALVASGIRGGPVRAGQQAM
                     SASYYAQAAGRSRLGALASGRAGGQVGSGAAGAAGGAATGGAVAAGAIAAKAVAGAAG
                     SGARAATASVDDVASRRPDGSAAGRPPRPTRRPPPGAEGEGDGGRT"
     gene            1129..>1707
                     /locus_tag="VF954_01320"
     CDS             1129..>1707
                     /locus_tag="VF954_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO6880 family protein"
                     /protein_id="HEX9529747.1"
                     /translation="MAAEREDRVYRFDPADASGVFLGLGLIQCALVGGGLVISVAALT
                     QGVPLPLAAVPLALGAAASFTRLGGYAAWEWLPLIGGWTWMRVGRGRRWFARLPLVPD
                     ADGGPTPLPPCLAGLSIIEVPWRGRQRLGAVRDAERHTLTAATTVAGPQFVVQSRPDQ
                     ERLLAGWGDILNQFAVERGVVTHLAWSDLAAPS"
BASE COUNT          176 a          658 c          609 g          264 t
ORIGIN      
        1 acttcttcct cggcgccacc gaccccaacg tgcaggccga ctggttcatc cgtggccacg
       61 gcccgtacgc gaccacggtc ggcatcgcgg ccacgctgct ggtcggcttc gtcctcatcg
      121 gcatcaccca gggcgtgctc accggcgacg tcgcgggaat gctgcgccgc atcaccttcg
      181 agctgcccgt ggcggtcctc gccatggtgg ggctgatcac ggtcactcaa gcactcatcc
      241 ggctcaccga cgagctctcg cgcgggctgc tcaagcagtt cggcgacgac gtgcaacact
      301 tcaccgccgg cgtcaactcg gtgtcggccc tcggcggccc gaccacgagc tcgttcgtcg
      361 tggtcgtcct ggctctggtg agtgtgctgg ccggcgtcgt gctcgtcgcc gagctcgtcg
      421 tgcgctccgc gctcatctac atcgtcgtgg ccctcgctcc gctcgtcttc gccgcccagc
      481 tgtggccggc cctgcgcggc gtggcccgca agctgctcga acttctcgtc gcgctcatcg
      541 tctccaagct cgtcgtggcc atcgcgctcg ccgtcgcggc ggctgcggcg gtcggcgccg
      601 gcgccggcgc cggcggccag gtcagctccc tgtcgccacc tgaggtcctc gcccacagcc
      661 ccgggggatc gatcacccag gcggtaggca tcctgctggc cgcgacggcc gccttcggtg
      721 tggccgcgtt ctcgccgttg ctgatcgccc ggctccttcc cctcaccgag gcggccttgg
      781 tcgcctccgg catacgcggc ggccccgtaa gggccggcca acaggccatg tcggccagct
      841 actacgccca ggcggcgggc cggtcgcgcc tcggcgctct cgcctcgggg cgcgccgggg
      901 gccaggtcgg aagcggcgcg gccggagccg ccggcggcgc ggcgacgggc ggggccgtgg
      961 ccgctggggc tatcgcggcc aaggcggttg ctggggcggc cgggagcggc gcgagggcgg
     1021 ctacggcctc cgtcgacgac gtcgcctctc gccgtcctga cggcagtgcc gccggcagac
     1081 cgccaagacc gactcgccgg ccgccacccg gcgcggaagg agagggcgat ggcggcagaa
     1141 cgtgaggacc gcgtctaccg gttcgacccg gccgacgcct ccggcgtgtt cttgggcctc
     1201 ggcctcatcc agtgcgcgct cgtcggcggc ggcctcgtga tctccgtcgc ggcgctcacc
     1261 cagggcgtgc ccctcccact ggccgccgtc cctctggccc tgggagccgc cgccagcttc
     1321 acccgcctcg gcggctatgc cgcctgggag tggctgcccc tgatcggagg gtggacgtgg
     1381 atgcgggttg gccgcggccg gcgctggttc gcccgcctgc ccttggtgcc cgacgccgac
     1441 ggcgggccga cgccgctgcc gccgtgcctc gccggcctgt cgatcatcga ggtgccgtgg
     1501 cgcggccgcc aacgcctggg ggctgtgcgc gacgccgagc ggcacacgct caccgccgcg
     1561 accacggtgg cggggccaca gttcgtggtg cagtcgcgtc ctgaccagga gaggctgctg
     1621 gcagggtggg gcgacatcct caaccagttc gccgtcgagc gaggagtcgt cacccacctc
     1681 gcctggtccg acctggcggc cccgtcg
//
LOCUS       DASWGG010000077         4912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1686625, whole genome shotgun sequence.
ACCESSION   DASWGG010000077 DASWGG010000000
VERSION     DASWGG010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686625"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1992
                     /locus_tag="VF954_01325"
     CDS             <1..1992
                     /locus_tag="VF954_01325"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEX9529748.1"
                     /translation="FDSPLDTAVEVNLLGPSRVAAAIAAAWEAAGRPTAEPAHLVAVS
                     TAYVAGTRRGTVREALLTDTPYAAEADWKAEVVAARRARADADATSRRPDALARFARQ
                     ARAELGAAGTPLLAERGEHHRREWVKQAMVDAGRARARALGWPDAYAYTKALGERALL
                     ESHGPVPITTVRPSIIESSLAEPRPGWIRGFRMAEPVIISYGRGLLKEFPGLPEGVVD
                     VIPVDLVVAAIIAVAATGPDPAGPTVYQVASGTRNPLRYRHLVDLVRAYFTEHPLYDT
                     EGQPIVVPQWSFPGRGRVQSQLTRAGKLLSAAERAVSSLPVRGQQAQLTARLEERRVQ
                     AERALGYVELYGAYAETEALFDVSRLLALSDRLDEADRAAFTLDPAAITWPRYVQGVH
                     LPAIVVHARVRTSGGRREGPSRFERGRRAILSPDRHLAVFDLENTLIASNVVESYAWL
                     ATRHLSTGDRARFTLKVLREAPNLLALDRRDRGDFLRHFYRRYEDAPAAQLGEDAWEL
                     FSHLILTKSFPAGVRRVREHRALGHRTLLITGALDFVIAPLRPLFDDVVCAQLGCDGD
                     RLTGELLEAPPTGEARAMVMADYAQAHGLDLREAVAYADSASDLPMLEASGHPVAVNP
                     EAKLATIARKRGWHVEQWHRAPGGPRPLLPIGPLIGATR"
     gene            1989..3191
                     /locus_tag="VF954_01330"
     CDS             1989..3191
                     /locus_tag="VF954_01330"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9529749.1"
                     /translation="MRALLFERSLPRFAAARVASALGAGWGPKVGPLRLAEIDRPEPP
                     AGAEGWHHVRPLLAGICGSDLATVEGRSSRYFEPIVSFPFVLGHEVVGITDDDRRVVI
                     EPVLSCAARAVDPPCPSCAAGHTGTCERVAFGHVKPGLQTGFCADTGGGWSTGLVAHL
                     SQLHPVPDALADEQAVLVEPTACAVHAALKASPPAGSLPTVAVIGAGTLGLLTIAALR
                     RYSLPGSILAGAKHPEQRRLALSLGADVVVEPGELARAVRRHSRSLAVGDRLTGGADV
                     VIDCVGTSDSIAEALAIVRPRGRVVLVGMPASVTVELTGLWHREVELVGAYAYGTEDA
                     GPTDDLGTRQHTFELAFALVADADLGRLLTATYPLDRWQDAIAHASAAGRRGAVKVAF
                     DLRKENAR"
     gene            3188..4777
                     /locus_tag="VF954_01335"
     CDS             3188..4777
                     /locus_tag="VF954_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX9529750.1"
                     /translation="MSPRPGFVLEVDRSTPPTLMWHGEGYRLEKLPVGSRVLYPPEPL
                     AAERYPDDAIRHALLNPLGDSAPLPELLRAGMRLTIAFDDVSLPLPPMRRPDVRQLVI
                     EAVLDLAADAGVDDVVLIAALALHRRMTEPELRHAVGDRIYEAFAPYGLLVQHDAEDP
                     DGMLHLGATDEGEDVEINKRAATSDLIVYVNINLVAMDGGHKSVATGLASYRSLRHHH
                     NVRTMLQSRSFMDQHHSELHSSNWRMGRHLAASGIKVFQIETTLNNQTFPEQFQFLQR
                     REWEWTARDRATFLASSKALERAPARVARKILHGIQAPYGLTGVHAGEVEAVHAATTE
                     NVHRQQLVDVEGQTDILTMGLPYICPYNVNSIMNPVLVMCLGLGYFFNLYRGRPLVRQ
                     GGVLILSHPTPWEFHPVHHPSYIDFFEQVLSETTDPVEIEAKYEEGFATDPWYVHLYR
                     TSYAYHGVHPFYMWYWGAHALQHLGGVIVVGGDVKAVRRLGFKPASTLRDALEMATDV
                     VGPDPSLTHLHAPPLALADVR"
BASE COUNT          666 a         1903 c         1674 g          669 t
ORIGIN      
        1 ttcgactccc ccctcgacac cgccgtcgag gtcaacctgc tcggcccgtc gagggtggcg
       61 gcggccatag ccgcggcgtg ggaggccgcc gggcggccca ccgccgagcc ggcccacctg
      121 gtcgccgtgt cgacggccta cgtggcgggc acccggcgcg gcaccgtgcg ggaggcgctg
      181 ctgaccgaca cgccctacgc cgccgaggcc gactggaagg ccgaggtggt ggccgccagg
      241 agggcgaggg cggacgccga cgccaccagc cggcgacccg acgccctggc ccgcttcgcc
      301 aggcaggcgc gcgccgagct gggcgcggcg ggcaccccgc tgctcgccga gcgcggcgag
      361 caccatcgcc gggagtgggt caagcaggcg atggtcgatg ccggccgggc ccgggcccgc
      421 gctctcggct ggccggacgc gtacgcctac accaaggcgc tcggcgagcg cgccctgctc
      481 gagagccacg gacccgtgcc catcacgact gtccggccct cgatcatcga gtcgtccctc
      541 gccgagcccc gcccgggctg gatccgcggc ttccgcatgg ccgagccggt gatcatctcg
      601 tatggccgcg gcctgctgaa ggagttcccc ggcctgcccg aaggcgtggt cgacgtcatc
      661 cccgtggacc tggtggtggc cgccatcatc gccgtggccg cgaccggccc cgaccccgcg
      721 gggccgaccg tgtaccaggt ggcatccggg accaggaacc cgctccggta ccgccatctg
      781 gtcgacctgg tgcgggccta cttcaccgag caccctctct acgacaccga aggccagccc
      841 atcgtggtgc cgcagtggtc cttccctggc cggggccggg tgcagagcca gctgacccgg
      901 gcaggcaagc tgctgagcgc ggcggagagg gcagtgtcgt ccctgcccgt ccggggccag
      961 caggcccagc tcaccgcccg cctggaagag cgccgggtcc aggccgagcg cgccctcggc
     1021 tatgtcgagc tgtacggcgc ctacgccgag accgaggcac tcttcgacgt gagccggctg
     1081 ctcgcgctct cggaccggct ggacgaagcc gaccgggccg cgttcacact cgaccccgcc
     1141 gccatcacgt ggccccgcta cgtccagggc gtgcacctgc cggccatcgt ggtgcacgcc
     1201 cgggtgcgca cctcgggcgg ccggcgggag ggaccgagcc ggttcgagcg cggcaggcgg
     1261 gccatcctgt cccccgaccg ccacctggcc gtcttcgacc tggagaacac cctcatagcc
     1321 tccaacgtcg tggagtccta cgcctggctc gccacccggc acctgtcgac cggggaccgg
     1381 gcccggttca ccctcaaggt cctgcgggaa gcgcccaacc tgctggcgct agaccgtcgc
     1441 gaccggggtg acttcctgcg ccacttctac cgccgctacg aggacgcccc cgccgcacag
     1501 ctgggcgagg acgcatggga gctgttctcc cacctgatcc tgaccaagtc gttcccggcc
     1561 ggggtacgcc gcgtccggga gcaccgggcg ctcggccacc ggacgctgct catcaccgga
     1621 gcgctcgact tcgtcatagc tccgctccgg ccgctgttcg acgacgtggt gtgcgcccag
     1681 ctcggctgcg acggcgaccg gctgaccggc gagctgctcg aggcgccgcc cacgggcgag
     1741 gcccgggcca tggtcatggc ggactacgcc caggcccacg gccttgacct ccgcgaggcc
     1801 gtggcgtacg ccgattcggc cagcgacctg cccatgctcg aagcctccgg ccatcccgtc
     1861 gccgtcaacc ccgaggccaa gctggccacc atcgcccgca agcggggctg gcacgtcgag
     1921 cagtggcaca gggcgcccgg cgggccccgc cccctcctgc cgatcgggcc cctgatcggg
     1981 gcgaccagat gagggccctc ctcttcgagc gatcactccc ccgtttcgcg gccgcccgag
     2041 tggcctcggc cctgggcgcc ggctggggcc cgaaggtggg cccgttgcgg ctggcggaga
     2101 tcgaccgccc cgagccgccg gccggggccg agggatggca ccacgtccgc ccgctgctgg
     2161 cgggaatctg cggatccgac ctggcgaccg tcgagggtcg cagctcccgg tacttcgagc
     2221 cgatcgtgtc gttcccgttc gtgctgggcc acgaggtggt gggcatcaca gacgacgacc
     2281 gccgcgtggt catagagccg gtgctgtcgt gcgcggcgcg agccgtcgac ccgccctgcc
     2341 cctcgtgcgc cgccggccac accggtacgt gcgagcgggt ggccttcggc cacgtcaagc
     2401 ccggcctcca gaccggcttc tgcgccgaca ccggtggggg ttggtccacc gggctggtgg
     2461 cccacctcag ccagctgcac ccggtgcccg acgccctcgc cgacgagcag gcggtgctcg
     2521 tggagcccac cgcgtgcgcg gtccacgccg ccctcaaggc ctcgcccccc gcaggctccc
     2581 tgccgaccgt ggccgtcatc ggcgcgggca ccctggggct gctgacgatc gcggccctgc
     2641 gccgctattc ccttcccggc tccatcctgg ccggggccaa gcacccggag cagcgtcgcc
     2701 tggcgctcag cctgggcgcc gacgtggtgg tggagcccgg cgagctggcc cgggccgtgc
     2761 gccgccacag ccggtcgctc gcggtcggcg atcgcctcac cggcggcgcc gacgtggtga
     2821 tcgactgcgt cggcacgtcc gactccatcg ccgaggcgct cgccatcgtg cgcccccggg
     2881 ggcgggtggt cctggtgggc atgccggcgt cggtcaccgt cgagctcacc ggcctgtggc
     2941 accgggaggt cgagctggtc ggggcgtacg cctacggcac cgaggacgcc gggcccaccg
     3001 acgacctcgg cacccgacag cacaccttcg agctggcctt cgccctcgtg gccgacgccg
     3061 acctgggccg gctgctgacc gccacctacc ccctggaccg ctggcaggat gccatcgccc
     3121 acgcgtccgc cgccggccgg cggggcgcgg tcaaggtggc cttcgacctc cggaaggaga
     3181 acgcccgatg agtcccaggc ccggcttcgt gctcgaggtc gaccgctcca ccccgccgac
     3241 gctgatgtgg cacggcgagg gataccggct ggagaagctg cccgtcggca gccgcgtgct
     3301 gtacccgccc gagccgctgg ccgccgaacg gtatcccgac gacgccattc gccacgccct
     3361 gctcaacccg ctcggcgaca gcgcgccgct gcccgagctg ctcagggcgg ggatgcggct
     3421 caccatcgcg ttcgacgacg tgtcgttgcc gctgccgccg atgcgccgcc ccgacgtccg
     3481 ccagctcgtc atcgaagccg tgctcgacct ggcggccgac gccggcgtgg acgatgtcgt
     3541 gctcatcgcc gcgctggccc tgcaccgccg catgaccgag ccagagctgc gccacgccgt
     3601 cggcgaccgc atctacgagg cgttcgcgcc gtacggcctg ctggtccagc acgacgccga
     3661 ggaccccgac ggcatgctgc acctgggcgc caccgacgag ggggaggacg tggagatcaa
     3721 caagcgggcc gcgaccagcg acctgatcgt ctacgtcaac atcaacctgg tggccatgga
     3781 cggcggtcac aagagcgtag ccaccggcct ggccagctac cgcagcctcc gccaccacca
     3841 caacgtgcgc acgatgctcc agagccggtc gttcatggac cagcatcaca gcgagctgca
     3901 ctcctccaac tggcgcatgg gccgccacct ggcggcctcg gggatcaagg tgttccagat
     3961 cgagaccacc ctcaacaacc agaccttccc ggagcagttc cagttcctcc agcggcggga
     4021 gtgggagtgg acggcccggg accgggccac cttcctggcc tcgtccaagg cgctggagcg
     4081 ggcgccggcc cgcgtggccc gcaagatcct ccacggcatc caggcgcctt acggcctcac
     4141 gggcgtccac gccggcgagg tggaggccgt ccacgcggcc acgaccgaga acgtccaccg
     4201 ccagcagctg gtcgatgtcg agggccagac cgacatcctg accatgggcc tgccctacat
     4261 ctgcccgtac aacgtcaact ccataatgaa cccggtcctc gtcatgtgcc tcggcctggg
     4321 ctacttcttc aacctgtacc ggggcaggcc gctcgtgcgc caagggggcg tgctgatcct
     4381 ctcccatccc accccgtggg agttccaccc cgtccaccac ccgagctaca tcgacttctt
     4441 cgagcaggtg ctgtccgaga cgaccgaccc ggtggagatc gaggccaagt acgaggaggg
     4501 cttcgcgacc gacccgtggt acgtccacct gtaccggacc tcgtacgcct accacggcgt
     4561 ccacccgttc tacatgtggt actggggtgc ccacgccctc cagcacctgg gcggcgtcat
     4621 cgtggtgggc ggcgacgtga aggccgtgcg ccggctgggc ttcaagccgg ccagcaccct
     4681 gcgcgacgcc ctcgagatgg cgaccgacgt ggtcgggccc gaccccagcc tcacccacct
     4741 ccacgccccg ccgctcgccc tggccgacgt ccgatgagta gccctatgtc aagccgcctc
     4801 gcagatgagg cgttggagcg cggtgtgctt ggcctcgtcg tagggcatgc ggtctgtcca
     4861 gcaccgccag atgatgtgcg cccagctgcg ggcgaggatc cgttcggcgt gg
//
LOCUS       DASWGG010000078         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2655568, whole genome shotgun sequence.
ACCESSION   DASWGG010000078 DASWGG010000000
VERSION     DASWGG010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2655568"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VF954_01340"
     CDS             <1..448
                     /locus_tag="VF954_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9529751.1"
                     /translation="LGEEADRIVGLELGADDYVAKPFSPRELTARVKAVLRRATGPLS
                     AGAQTPAVLRAGELEVDITAHEARLGGELVALTAKEFDLLAYLMEHPRRAFRREELLE
                     QVWGFSYGDTSTVTVHIRRLREKIETDPSAPRYVTTVWGVGYRFES"
     gene            445..>1599
                     /locus_tag="VF954_01345"
     CDS             445..>1599
                     /locus_tag="VF954_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX9529752.1"
                     /translation="MSAPRWTPLAVGFAGFAAAVCAALAGGFTGDDAARLVLYSAVGA
                     ALAGVVAGLLLHALRRAAIGVQAAVAALAAVLAVAIGVAWASAQMFLMKHDLRVLWVV
                     LISAGTVGLIVAIVFGRRVAAASRSVGEMARRLGESGFEGDFTLTTGAGTRRVPGELA
                     SLAAELEQTSARLAEAQAQASALERSRRELVAWVSHDLRTPLAGVRAMVEALEDRVVD
                     DPETVARYYATMRREIDRLAGLVNDLFELSRIQSGSLALDIESVPLDELVGDAVAGAA
                     IAASAKGIDLRGEVGEPSPVVELSTPEMVRVVRNLLDNAIRHTPPGGVVVITAGLDAS
                     GEAAEVSVQDACGGIPERELDSVFEMAYRGDAARTPGHGGGGLGLAVARGL"
BASE COUNT          178 a          576 c          619 g          226 t
ORIGIN      
        1 cctcggtgag gaggccgacc gcatcgtcgg cctcgagctc ggcgccgacg actacgtggc
       61 caagccattc tcgccccgag agctcacagc ccgggtcaag gccgtcctcc gccgggcgac
      121 cgggcccttg tcggccggcg cccagacccc ggcggttctg cgggccggcg agctggaggt
      181 cgacatcacc gcccacgagg cccgcctcgg cggcgagctg gtggccttga cggccaagga
      241 gttcgacctc ctggcctatc tcatggagca tccccggcgg gccttccggc gggaggaact
      301 cctggagcag gtgtggggct tcagctacgg cgacacctcg accgtcaccg tgcacatccg
      361 ccgcctgcgg gagaagatcg aaaccgaccc ctcggcgccc cgctacgtca cgacggtgtg
      421 gggcgtcggc taccggttcg agtcgtgagc gcgccgcgct ggaccccgct ggcggtgggc
      481 ttcgccgggt tcgccgccgc ggtctgcgcc gctctggccg gcgggttcac cggcgatgac
      541 gccgctcgcc tcgtcctcta ctccgcggtg ggggcggcac tggccggcgt cgtggccggg
      601 ctcctgctcc acgccctgcg gcgggccgcc atcggcgtgc aggcggcggt cgccgcgctg
      661 gcggcggtgc tggcggtggc cattggtgtc gcctgggcgt cggcgcagat gttcctcatg
      721 aagcacgacc tccgggtgct gtgggtcgtc ctgatcagcg ccggcaccgt cgggctgatc
      781 gtggccatcg tgttcggccg ccgggtggcg gcggccagcc ggtcggtggg ggagatggcc
      841 cgccggctgg gcgagtcggg cttcgagggc gacttcacgc tcaccaccgg ggcggggacc
      901 cggcgggttc ccggcgagct ggccagcctg gccgccgaac tggagcagac ctccgcccgt
      961 ctggccgagg cccaggcgca ggcgtcggcg ctggaacgga gccgccggga gctggtggcc
     1021 tgggtgtcgc acgatctccg cacgccgctg gccggggtcc gggcgatggt cgaggcgctg
     1081 gaggaccggg tcgtcgacga ccccgagacc gtggcccgct actacgccac gatgcggcgg
     1141 gagatcgacc ggctggccgg cctcgtgaac gacctgttcg agctgtcccg catccagtcg
     1201 ggctcgctgg ccctcgacat cgagtcggtg cccctcgacg aactcgtcgg cgacgccgtg
     1261 gccggcgccg ccatcgccgc ctcggccaag gggatcgacc tgcggggcga ggtcggcgag
     1321 ccgtcgccgg tggtggagct gtcgacgccg gagatggtcc gggtggtgcg gaacctgctc
     1381 gacaacgcca tccgccacac gcccccgggc ggtgtcgtcg tgatcaccgc cgggctcgac
     1441 gccagcggcg aggcggccga ggtgtcggtg caggacgcct gcggcgggat ccccgagcgg
     1501 gagctcgact cggtcttcga gatggcctac cggggcgacg ccgcccgcac ccccggccac
     1561 ggtggcggcg gcctcggcct ggccgtggcc cgggggctg
//
LOCUS       DASWGG010000079         4361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_724159, whole genome shotgun sequence.
ACCESSION   DASWGG010000079 DASWGG010000000
VERSION     DASWGG010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724159"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..865
                     /locus_tag="VF954_01350"
     CDS             89..865
                     /locus_tag="VF954_01350"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX9529753.1"
                     /translation="MTGPSPEGERLQKVLARAGVASRRAVEELISEGRVTVNGAAAVL
                     GRRVDPERDRVEVDGVRVVLAPGAVHYLLNKPSGTVATASDPQGRPTVVQLVPPVPRV
                     FPVGRLDADTEGLLLLTNDGDLAHRLAHPSFGVDKEYLAHVAGSLSPGDLRRLRTGVE
                     LEDGRTAPARVAQVAPGVLRITIHEGRNRQVRRMCEAVGHPVRRLVRTRIGPIRDRGL
                     GPGQWRRLAPSEVQALEAALASAATAASASGAAGAAGPSR"
     gene            877..1242
                     /gene="aroH"
                     /locus_tag="VF954_01355"
     CDS             877..1242
                     /gene="aroH"
                     /locus_tag="VF954_01355"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX9529754.1"
                     /translation="MAAVRALRGATTVDEDTVGHVNERVQALIQCMLERNDLGHDDLI
                     SILFTATEDVVSTFPATAARAVGLGDVPLICARELAVVGATPRCIRVMMHVGTDTPRD
                     QLHHVYLEGARGLRDDLPD"
     gene            1226..2323
                     /locus_tag="VF954_01360"
     CDS             1226..2323
                     /locus_tag="VF954_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX9529755.1"
                     /translation="MTSPTEPRRAGIVGTGLIGGSIGMALRARGWHVSGFDRDPDRAA
                     RAVALGALDEVGPMAGAEVVFVATPVGAITGAATAALAGAGPGGAGEAGPVVTDVGSV
                     KASVVDAIGHPRFVGGHPMAGSEQEGVEGADAELFAGATWVLTPTAATDPGAYARVHA
                     VVSSFGADVVAVAPERHDALVAMVSHVPHLTAAALMELAAADAEEHVALLRMAAGGFR
                     DMTRVAAGHPGIWPDICAENAGAILATLDRLVGRLSAVREVVARADRGALLEMLESAR
                     TARRNLPARAALRAEELVEVRVPVPDREGVLAEVTTLAAELGVNIWDLEIAHSAEGDR
                     GVLLMVVDAAAADLVRGALLARGYRPSVQPL"
     gene            2320..2934
                     /locus_tag="VF954_01365"
     CDS             2320..2934
                     /locus_tag="VF954_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529756.1"
                     /translation="MTGPARRIEPLAGRVDAEVVVPGSKSITNRALLAAGLAAGTSRL
                     RGILVAEDTDAMVSCVTALGASVTPGPGATTVTVSGVGGRLPAGPRTLHAARSGTTAR
                     FVAAACAAGPGPYRVDGAEQMRRRPMGPSFDALRALGATVREQGAAGHLPAVISGPAR
                     GGTVRVRGDVTSQSPRACCWRGRCSGTAYGSSSPRRWCPGPTSS"
     gene            2907..3611
                     /locus_tag="VF954_01370"
     CDS             2907..3611
                     /locus_tag="VF954_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529757.1"
                     /translation="MVSRPYVELTAGVMGSFGAEVGMADGAFSVAPGGAGYLAAASYE
                     VEPDASGAAYFWAAAAMCGGRARVLGLGPASRQADVAFIDVLEAMGAEVDRAPAGICV
                     ARGSPLHGIEVDLAHLSDTAPTLAVVAALADGPTTVRGIGFIRRKESDRIAAVVTELR
                     RCGIGADELPDGFVVRPAPVRPARVRTYDDHRMAMSFALLGLARPGIEIEGPGCVAKT
                     FPTFWEDLEQLRGRHG"
     gene            3604..4245
                     /gene="cmk"
                     /locus_tag="VF954_01375"
     CDS             3604..4245
                     /gene="cmk"
                     /locus_tag="VF954_01375"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX9529758.1"
                     /translation="MGSAAGQVIAIDGPAGSGKSTVAARVAHSLGWAHLDTGAMYRAV
                     TWAVLRAGLDAADGDAVAALARALPIEVGPPVIVDGQDVTGAIRGPEVSGAVSVVAAH
                     PGVRQDLVRRQRVWVADHGGGVVEGRDIGSVVFPRAELKVYLTASDAERAMRRAGQDV
                     GMGRHAAAADIARRDLLDSARAASPLQVADGAVVIDTTGRPVDDVVEEVLAHL"
BASE COUNT          474 a         1636 c         1689 g          562 t
ORIGIN      
        1 actcgctcgc gcagctcccc cctctcgggg acttcgtgcc cggaccggag gtcgtcgagg
       61 cgctcgaaca ggggctgcgc ccggccggat gaccggtccc tcgcccgagg gggagcggct
      121 ccagaaggtt ctcgcccggg ccggcgtcgc cagccggcgc gccgtggagg agctcatcag
      181 cgagggccgg gtcaccgtca acggcgcggc tgccgtgctc ggtcggcggg tggaccccga
      241 gcgggaccgg gtcgaggtgg acggcgtccg cgtcgtgctg gcacccggcg ccgtccacta
      301 cctgctcaac aagccgtcgg gcacggtggc cacggcgtcg gacccgcagg gtcgccctac
      361 ggtggtgcag ctggtcccgc cggtgccccg ggtgttcccg gtcgggaggc tggacgcgga
      421 caccgagggg ctgctcctgc tgaccaacga cggcgacctg gcccaccgcc tggcccatcc
      481 gtccttcggc gtggacaagg agtacctggc ccacgtggcc ggctcgctct caccggggga
      541 cctgcgccgc ctgcgcacgg gcgtcgagct cgaggacggc cgcaccgcgc cggcccgggt
      601 ggcccaggtc gcgcccgggg tgctgcgcat cacgatccac gagggccgca accgccaggt
      661 gcggcgcatg tgcgaggccg tgggccaccc cgtgcgccgc ctggtccgca cccggatcgg
      721 ccccatccgc gaccgcggcc tcgggcccgg gcaatggcgc cggctggcgc cgtcggaggt
      781 gcaggcgctc gaggcggccc tggcctcggc cgccactgcc gcctcggcct ccggggccgc
      841 gggggcggcg gggcccagtc gctagtctcg gggcccatgg ccgcggttcg cgccctccgg
      901 ggggccacca ccgtcgacga ggacacggtc gggcacgtga acgagcgcgt ccaggctctc
      961 atccagtgca tgctggagcg caacgacctg ggccacgacg acctgatcag catcctgttc
     1021 accgccacgg aggacgtcgt gtcgaccttc cccgcgaccg cggcccgcgc cgtcgggctg
     1081 ggcgacgtgc cgctcatctg cgcccgggag ctggccgtgg tcggggccac gccgcggtgc
     1141 atccgcgtca tgatgcacgt cggcacggac accccccgcg accagctcca ccacgtgtac
     1201 ctggaggggg cgcggggctt gcgcgatgac ctccccgact gagccgcgcc gggccgggat
     1261 cgtcggcacc ggcctgatcg gcgggtcgat cggcatggcg ctgcgagccc ggggctggca
     1321 cgtctccggc ttcgaccgcg accctgaccg ggccgcccgg gcggtggccc tcggcgccct
     1381 cgacgaggtc ggccccatgg ccggcgccga ggtggtgttc gtggccacgc ccgtgggcgc
     1441 catcactggt gccgccacgg ccgccctggc cggggccggt ccgggtgggg ccggcgaggc
     1501 cggcccggtc gtgaccgacg tcggcagcgt gaaggcgtcg gtggtcgacg ccatcgggca
     1561 cccgcgcttc gtcggcggcc accccatggc cggctccgag caggaagggg tggagggcgc
     1621 cgacgccgag ctgttcgccg gtgccacctg ggtcctgact cccaccgcgg ccaccgaccc
     1681 cggcgcctac gcccgggtgc acgccgtggt ctcgtccttc ggggccgacg tggtggccgt
     1741 ggcccccgag cgccacgacg ccctggtggc catggtctcc catgtgcccc atctgacggc
     1801 tgcggcgctc atggagctgg ccgccgccga cgccgaggag cacgtcgccc tgctccggat
     1861 ggcggcgggc ggcttccggg acatgacgag ggttgccgcg ggccatcccg ggatctggcc
     1921 ggacatctgc gcggagaacg caggagccat cctggcgacc ctcgaccgtc tcgtgggccg
     1981 gctgtcggcg gtgcgcgagg tggtggcccg ggccgaccgg ggcgccctcc tcgagatgct
     2041 ggagtcggcg cgtacggccc ggcggaacct gccggcccgg gccgccctgc gggccgagga
     2101 gctggtcgag gtccgcgtcc cggtgcccga ccgcgaaggc gtcctggccg aggtcaccac
     2161 cctggcggcg gagctcgggg tgaacatctg ggacctggag atcgcccact cggccgaggg
     2221 cgaccggggg gtcctgctca tggtggtgga cgccgcggcc gccgatctgg tgcggggcgc
     2281 cctgctcgcc cggggctacc gcccctcggt gcagccgctg tgaccgggcc cgctcgccgg
     2341 atcgagccgc tggccgggcg ggtggacgcc gaggtggtcg tgcccgggtc caagagcatc
     2401 accaaccggg ccctgctcgc cgccggcctg gccgccggga ccagccggct gcgaggcatc
     2461 ctggtggccg aggacaccga cgccatggtg tcgtgcgtga ccgccctggg ggcctcggtc
     2521 actcccggcc ccggagccac caccgtcacg gtgtccggtg tcggtggccg gctgcccgcc
     2581 ggcccccgga ccctgcacgc ggcccggtcg ggcacgacgg cgcgcttcgt cgccgcggca
     2641 tgcgccgccg ggcccggccc gtaccgcgtc gacggagccg agcagatgcg ccggcggccg
     2701 atgggcccct ccttcgacgc cctgcgagcc ctgggcgcga ccgtccggga acaaggcgcg
     2761 gccggccacc tgccggcggt gatctccggg ccggcccggg ggggcacggt tcgcgtccgg
     2821 ggcgacgtca ccagccagtc ccctcgggcc tgctgctggc ggggccgctg ttccgggacg
     2881 gcatacgggt cgagctcacc acgccgctgg tgtcccggcc ctacgtcgag ctgaccgccg
     2941 gggtaatggg cagcttcggg gccgaggtcg ggatggccga cggcgccttc tcggtcgcgc
     3001 ccggcggcgc cggctacctg gcggccgcgt cctacgaggt ggagcccgac gccagtgggg
     3061 ccgcctactt ctgggccgcg gccgccatgt gcggcggccg ggcccgggtg ctcggcctgg
     3121 gcccggcctc acggcaggcc gacgtcgcct tcatcgacgt cctggaggcc atgggcgccg
     3181 aggtcgaccg cgccccggcc ggcatctgcg tggccagggg gagccccctg cacggcatcg
     3241 aggtcgacct ggcccacctg tccgacaccg cgcccaccct cgccgtggtc gccgccctcg
     3301 ccgacgggcc gaccaccgtg cggggcatcg gcttcatccg gcgcaaggag tcggaccgga
     3361 tcgcggcggt ggtcacggag ctgcggcgct gcgggatcgg cgccgacgag ctgcccgacg
     3421 gcttcgtggt gcgccccgcg ccggtccggc ccgcccgggt gcgcacctac gacgaccacc
     3481 ggatggccat gagcttcgcc ctgctgggcc tggcccggcc gggcatcgag atcgagggcc
     3541 cggggtgcgt ggccaagacc ttccccacct tctgggagga cctggagcag ctccgggggc
     3601 gccatgggta gcgcggccgg tcaggtcatc gccatcgacg gcccggcagg gtccgggaag
     3661 tcgacggtcg cggcccgggt ggcgcacagc ctgggatggg cccacctgga caccggcgcc
     3721 atgtaccggg cggtcacgtg ggctgtgctg cgcgccggcc tcgacgccgc ggacggcgac
     3781 gccgtggccg cactggcccg agcgctgccg atcgaggtcg ggccgcccgt gatcgtcgac
     3841 ggacaggatg tcacgggggc cattcggggg cccgaggtca gcggggcggt gagcgtggtc
     3901 gcagcccatc cgggcgtgcg ccaggacctg gtccgtcggc agcgggtctg ggtggccgac
     3961 cacggtggcg gggtggtgga gggccgggac atcgggtccg tcgtgttccc tcgggccgag
     4021 ctgaaggtgt acctgacggc gtcggacgcc gagcgggcca tgcggcgcgc cgggcaggat
     4081 gtcggcatgg gacgtcacgc cgccgcggcc gacatcgccc gccgggacct gctcgactcc
     4141 gcccgggcgg cctcaccgct gcaggtggcg gacggcgccg tcgtcatcga caccaccggc
     4201 cggcccgtcg acgacgtggt cgaggaagtc ctggcccacc tgtgagcagg ccaccgggtc
     4261 cctctcgcat ctcgctcctt ttctaccggt gcatccgcaa cctggtggtc gccttctgcc
     4321 ggatctactg gcgcgagcag gtgaccggca ggcagcacgt g
//
LOCUS       DASWGG010000080         3931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_725328, whole genome shotgun sequence.
ACCESSION   DASWGG010000080 DASWGG010000000
VERSION     DASWGG010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725328"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /gene="malQ"
                     /locus_tag="VF954_01380"
     CDS             <1..1223
                     /gene="malQ"
                     /locus_tag="VF954_01380"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX9529759.1"
                     /translation="ALNRHRRVDRDRVWSLKGAALEAIWAASQPVVDREFEAFCDRSG
                     ASLAGYATFCVLTEELGAPWTAWPAPFRSHDHPAVAALVARRADRVRFHQWLQWLLDR
                     QLVRVGGQLGLLQDLAIGLDPEGADVWCWPEGFPAGVQVGAPPDEFNTRGQGWGLAPF
                     NPWALRAAGFEPFIQTVRAGFRHAGGLRFDHVMGLFRLFWIPPGGGPAQGAYVRYPAK
                     ELLDILALESVRAQAYVVGEDLGTVEPSVREELARRGVLGYRVMWFEPGLPATYAEQS
                     LAAVSTHDLPTVAGLWSGSDLRAQVDLGLDPDLAATGAVRRKLRRWAALADDAPPDEA
                     VRGAYRLLADAPSTLRVASLDDALAVEERPNMPGTVDAWPNWSLALPEPLEEIEAHPG
                     VAEIARILGEVSAD"
     gene            1251..2327
                     /locus_tag="VF954_01385"
     CDS             1251..2327
                     /locus_tag="VF954_01385"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX9529760.1"
                     /translation="MELPVMPPVSPMLAKLARQLPEGEFVYEPKWDGFRCIVFRDGAE
                     VELGSRNERPLTRYFPELADPIRASLPDKAVLDGEIVIAGPAGLDFDALQLRLHPADS
                     RVRMLAAQIPARFVAFDVLGLGAADLRAVPFAERRARLEEALGAAQPPVHLTPATRDV
                     GVARDWFERFEGAGLDGVVAKRTTLPYREDERVMIKVKHERTADCVVAGFRWHKSGPI
                     VGSLLLGLYDEHRTLHHLGVTASFTMARRRELVEELAPYRDHALAGHPWEGWAAAEEE
                     GARAGRMPGGSSRWNAGKDLRWEPIRAELVCEVAYDHLQRDRFRHATTFRRWRPDRDP
                     RTCTYAQLEAAVPAELESVFASKG"
     gene            complement(2347..3807)
                     /locus_tag="VF954_01390"
     CDS             complement(2347..3807)
                     /locus_tag="VF954_01390"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX9529761.1"
                     /translation="MAGTAALAVAALGVLPVAAAAAPVTPRPAAYGRDFPDPFVLWTG
                     TSYDAFATQGQGRNVQVITSPDLAQWSALPDALPQLPSWSEPGFVWGPSVLRRPGGYV
                     LYYATRVVGNGLQCISRATSATPAGPFRDTTPIATICQGDHGGSIDPSPFVDADGTPY
                     LLWKSEGVVNQEPPMIWAQRLTDDGLDVVGSPTVLLRQDQPWEYPIIEGPTMVRHGGQ
                     HWLFYGGNQWASADYAIGMASCASVVGPCTKQGDAPVLSTSGDMAGPGGPETFSDQAG
                     NLWFSYHAWGAGAVGYPQGQRLMHLAELGFPGGWPALDGVSLRPEPDPGGYRLVAADG
                     GVFAFGTAAFAGCACPRPGPVVGGAATPDGGGYWWAAADGSVGAFGDAPDAGSAAGIP
                     LTLPVVAMASTAPGHGYWLVAGDGGIFAFGDAAFHGSTGAMRLNRPVVGMAPTPSGGG
                     YWLVASDGGIFAFGDAGFHGSAGGIALAQPVVALIP"
BASE COUNT          502 a         1425 c         1500 g          504 t
ORIGIN      
        1 gggccctcaa ccggcaccgc cgggtggacc gggaccgggt gtggtcgctc aagggggccg
       61 ccctcgaggc catctgggcg gcgtcccaac cggtggtcga cagggagttc gaggccttct
      121 gcgatcggtc cggggcgtcg ctggcgggct atgccacctt ctgcgtgctg accgaggagc
      181 tcggcgcgcc gtggaccgcg tggcccgctc ccttccggag ccacgatcac ccggccgtgg
      241 cggctctagt ggcccgacgg gccgatcggg tgcgcttcca ccagtggctc cagtggctcc
      301 tcgaccgcca gctggtccgg gtcggggggc agctcggcct gctccaggac ctggccatcg
      361 gcctcgaccc ggagggcgcg gacgtgtggt gctggccgga gggctttccc gcgggggtcc
      421 aggtgggggc gcccccggac gagttcaaca cccggggcca gggctggggg ctggcgccat
      481 tcaacccgtg ggcgctgcgc gccgcggggt tcgagccctt catccagacc gtgcgcgccg
      541 gcttccgcca tgccggcggg ttgcggttcg accacgtgat ggggctgttc cggctcttct
      601 ggatccctcc cggcggcggc ccggcgcagg gcgcgtacgt ccggtacccg gcgaaggagc
      661 tgctggacat cctggccctg gagagcgtcc gggcccaggc ctacgtggtg ggcgaggacc
      721 ttggaacggt cgagccctcg gtccgggagg agctcgcccg ccggggtgtg ctcggctacc
      781 gggtgatgtg gttcgagccg ggtctgcccg ccacctacgc cgagcagtcg ctcgccgccg
      841 tcagcacgca cgatctgccg accgtggccg gcctgtggtc cgggtccgac cttcgggccc
      901 aggtcgacct cgggctggac cccgacctgg ccgcgaccgg ggccgtccgc cggaagctcc
      961 ggcgctgggc ggcgctcgcc gacgacgccc cgcccgacga ggcggtccgg ggcgcctacc
     1021 gcctcctggc cgacgcccct agcacgttgc gcgtggcgtc cctcgacgac gccctcgccg
     1081 tggaggagcg ccccaacatg ccgggcacgg tcgacgcctg gcccaactgg tcgctcgccc
     1141 tgccggagcc tctggaggag atcgaggccc accccggggt ggcggagatc gcccggatcc
     1201 tcggggaggt ctcggcggat tgactcgacg ggcgtggcat ggtgaggggc gtggagctgc
     1261 ccgtcatgcc gcccgtgtcg cccatgctgg ccaagctcgc ccgccagctg ccggagggcg
     1321 agttcgtcta cgagcccaaa tgggacggct tccggtgcat cgtcttccgg gacggcgcgg
     1381 aggtcgagct cggcagccgc aacgaacggc cgctgactcg atatttcccc gagctcgccg
     1441 acccgatccg ggcctcgttg cccgacaagg cggtgctgga cggggagatc gtcatcgccg
     1501 gaccggccgg cctggacttc gacgccctcc agctgcgcct ccaccccgcc gactcgaggg
     1561 tccggatgct cgccgcccag atcccggccc gcttcgtcgc cttcgacgtg ctcggtctcg
     1621 gcgccgccga tctccgggcc gtcccgttcg ccgagcgccg ggcgcggctg gaggaggcgc
     1681 tcggcgccgc ccagcctccc gtccatctca cgccggccac ccgggacgtg ggcgtggccc
     1741 gggactggtt cgagcgcttc gagggcgccg ggctcgacgg cgtggtggcc aagaggacca
     1801 cgctgccgta ccgggaggac gagcgggtga tgatcaaggt caagcacgag cggaccgccg
     1861 actgcgtcgt ggccggcttc cgctggcaca agtcggggcc gatcgtgggc tcgctcctgc
     1921 tgggcctcta cgacgagcac cgaaccttgc accatctcgg ggtgacggcg tcgttcacca
     1981 tggcccggcg ccgggagctg gtggaggagc tggcgcccta ccgggaccac gccctcgccg
     2041 gccatccctg ggagggctgg gcggccgccg aggaggaggg ggcccgggcc gggcggatgc
     2101 cgggcgggtc cagccgctgg aacgccggca aggacctgcg gtgggagccc atccgggccg
     2161 agctggtgtg cgaggtggcc tacgaccacc tccagcggga ccggttccgc catgccacca
     2221 cgttccgccg gtggcgcccc gaccgcgacc cccgcacgtg cacgtacgcc cagctcgagg
     2281 cggcggtgcc cgccgagttg gagtcggtgt tcgcctcgaa gggttgagcc ctcgaccgag
     2341 agggccctac gggatcaggg ccacgaccgg ctgggccagg gcgatgccgc ccgccgagcc
     2401 gtggaagccg gcgtctccga aggcgaagat ccccccgtcg ctggcgacca gccagtaccc
     2461 gccgccagag ggcgtgggcg ccatgccgac gacgggccgg ttgaggcgca tggcgccggt
     2521 ggagccgtgg aaggcggcgt ctccgaaggc gaagatcccc ccgtcgcccg ccaccagcca
     2581 gtacccgtgc ccgggcgcgg tcgaggccat ggccaccact gggagcgtga gcgggatgcc
     2641 agcggcgctg cccgcgtcgg gggcgtcgcc gaaggccccc accgagccgt cggccgccgc
     2701 ccaccagtag ccgccgccat cgggggtggc cgccccgccg acgaccgggc ccgggcgcgg
     2761 gcaggcgcag ccggcgaagg cggcggtgcc aaaggcgaac acgccgccgt cggccgccac
     2821 cagccggtag ccgccgggat ccggctcggg ccgcagcgag accccgtcga gcgccggcca
     2881 gccgccgggg aagcccagct cggccaggtg catgaggcgc tggccctgcg ggtagccgac
     2941 ggcaccggcc ccccaggcgt ggtaggagaa ccacaggttg cccgcctggt cgctgaacgt
     3001 ctcgggcccg ccggggccgg ccatgtcgcc cgaggtggag aggacgggcg catccccctg
     3061 cttggtgcac ggaccgacca ccgacgcgca cgaggccatg ccgatggcgt agtcggcgct
     3121 cgcccactgg ttgccgccgt agaacagcca gtgctggccg ccgtggcgca ccatggtcgg
     3181 cccctcgatg atcgggtact cccacggctg gtcctgcctg agcagcacgg tcggcgaccc
     3241 gacgacgtcc aggccgtcgt cggtcaggcg ctgggcccag atcatcggcg gttcctggtt
     3301 gacgacgcct tcggacttcc acagcaggta cggcgtcccg tcggcatcca cgaacgggct
     3361 cgggtcgatg gaccccccgt ggtcgccctg gcagatggtg gcgatcgggg tggtgtcgcg
     3421 gaagggcccg gcgggggtcg ccgaggtggc ccgggagatg cactggaggc cgttgccgac
     3481 cacacgggtc gcgtagtaca gcacgtagcc accgggccgg cgcagcaccg aggggcccca
     3541 gacgaagccg ggctcggacc acgacgggag ctgggggagc gcgtcgggaa gggcagacca
     3601 ttgggccagg tcgggggagg tgatcacctg gacgttgcgg ccctggccct gggtggcgaa
     3661 ggcgtcgtag gacgtcccgg tccacagcac gaaggggtcg gggaaatccc ggccgtaggc
     3721 cgcgggacgg ggtgtgacgg gcgcggccgc cgcggctacg gggagcaccc cgagcgcggc
     3781 gaccgccaac gccgcggtgc ctgccaccca ccgactcctt cgccgccgca acaccacaga
     3841 cccccggtcg ttcgagctcc tcaaatggta gccgtcaggc gctggccgga ccgccgcgat
     3901 ggcggaacgc gccggggccg gcgagcacgt g
//
LOCUS       DASWGG010000081         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1050183, whole genome shotgun sequence.
ACCESSION   DASWGG010000081 DASWGG010000000
VERSION     DASWGG010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050183"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..>1559
                     /gene="panC"
                     /locus_tag="VF954_01395"
     CDS             246..>1559
                     /gene="panC"
                     /locus_tag="VF954_01395"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX9529762.1"
                     /translation="MSRALASAGWTVSGFLGRGDDLAGAAEAVDLLIIATPDGAISKV
                     AEAVQPVPDTVVAHLAGSLGPDVLAPHPRRAGIHPLVSLPNEETGAARLAGAWFGVAG
                     EPMVHEIVDALGGRSIEVPDDARSTYHAAAAIASNHLVALLGQVQRVAAQAGIPLEAY
                     LDLVRGTVDNVAALGPAAALTGPVARRDWETVARHLAALPADERTAYQAMANEAARLG
                     GRERPGVGFGPLPGRRSRQPWSTRMPLTVLDRADSFRKALDAERQAGHSVGLVPTMGY
                     LHAGHTSLMQRAAAECDVVAATSFVNPLQFAPGEDLAAYPRDPEGDQAKAAAAGVEIL
                     FAPPTDEMFPEPPLTAVTVASLSDTLEGASRPSHFAGVATVVTKLFALAGPCRAYFGE
                     KDFQQLAVIRRLVQDLSLPVEVVGCPTVRDSDGLALSSRNAYLSAE"
BASE COUNT          221 a          574 c          536 g          228 t
ORIGIN      
        1 aacgagcctg acctgatcgt tccccacccc cgcatgtggg accgggcctt cgtgctcgtc
       61 ccgctgagcg acctggctcc ggacctggtc ggcgacaaac ggcccgacct gcttgcatcg
      121 ggggtccgtc ccgccggtaa cctgtaggca cacgaacggc acccgagagt cggggccgga
      181 acgtcagcca ggagcgtacc gttttgcgcc tccgcatcat cgggcccggc cgcgccgggc
      241 gagccatgag ccgggcactc gcgagcgcgg gctggaccgt gagtggcttc ctcggccgcg
      301 gcgacgacct cgccggtgcg gccgaggccg tcgatctcct catcatcgcg acacccgacg
      361 gcgctatctc gaaggtcgcc gaggcggtgc agccggtccc ggacaccgtg gtcgcccacc
      421 tggccggctc gctggggccg gacgtcctgg cgccccatcc tcgccgggcc gggatccatc
      481 ccctggtctc gctgccgaac gaggagacag gcgcagctcg cctcgccgga gcctggttcg
      541 gagtcgcggg cgaacccatg gtccacgaga tcgtcgatgc cctcggggga cgctcgatcg
      601 aggtgccgga cgacgcccgg tccacctacc acgccgccgc cgcgatcgcg tccaaccatc
      661 tcgtcgccct cctcggtcaa gtgcaaaggg tcgccgccca ggccgggata ccgctggagg
      721 cctacctcga cctggtcagg ggcacggtcg acaatgtcgc ggccctcggg cccgccgcgg
      781 cgttgaccgg cccggtcgct cggcgtgact gggaaactgt ggcgcgccac ctggccgccc
      841 tgccggccga cgagcgcacc gcctaccagg ccatggccaa tgaggccgcg agattgggtg
      901 gaagggaacg gccgggcgtg gggtttgggc ccctcccggg gcggcgaagc cgccagccat
      961 ggagtacccg catgccgctg acggtgctcg atcgggccga ttccttccgc aaggcgctgg
     1021 acgccgagcg gcaggccggc cactcggtag gacttgttcc caccatgggc tacctccacg
     1081 ccgggcatac gtcgctcatg caacgggcgg cggccgagtg cgacgtcgtg gcggcgacga
     1141 gcttcgtcaa cccgctccag ttcgcgccgg gcgaggacct ggctgcctat ccccgggacc
     1201 ctgaaggtga tcaggcgaaa gcggccgccg ccggcgtcga gatcctgttt gcccctccca
     1261 ccgacgagat gttcccggag ccgccgctga cagccgtcac cgtggcgagt ctctccgaca
     1321 ccctcgaagg agcatcccgg ccttcccact tcgccggcgt cgcgacggtg gtcaccaagc
     1381 tgttcgctct ggcggggccg tgccgggcct acttcggaga gaaggacttt cagcagctgg
     1441 cggtcatccg ccggctggtc caggacctgt ccctgccggt cgaggtcgtg ggatgcccga
     1501 cggtacgaga cagcgacggc ctggcactgt ccagccggaa cgcctacctg tcagccgag
//
LOCUS       DASWGG010000082         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1050669, whole genome shotgun sequence.
ACCESSION   DASWGG010000082 DASWGG010000000
VERSION     DASWGG010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050669"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="VF954_01400"
     CDS             <1..1585
                     /locus_tag="VF954_01400"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529763.1"
                     /translation="VQQGTSLDVIELDAASNSGVDAMRDLVSRAALATPGRWKVYIVD
                     EVHMLSSAASNALLKTLEEPPGHVVFVLATTDPQKVLMTIRSRTQHFEFRLLGGEILT
                     GLLREVRDRAGLEVPMEAVEVAVRKGHGSARDALSALDQVAAAGEVPDEVAVLDEVVD
                     ALIERDPSRALVAVAEVTAQGRDVQRVAVELVEHLRNVFLATMAPGLVPLPDDALVHL
                     QEQAHRLGTAALVRAIEVVGASQVDMRDAVDPRICLEVALVRLARPDADVSLAALQER
                     LERVEQRVTGHVAEPARTAARQAVTDPSARTQPPASAPTPAGTRADAGSAPSPSGAGP
                     MTGAAAARLALGGVRNKAGAAPPEISRSGPPPTSGPRPARPAGDAIARGAPGGLPSRD
                     ELTKVWGDLLLGQLPPRARARFGPGRFVEVDDRAAVFALPNDVHRARCEEVRAEVEQV
                     LSAHFGVPVPLSLVTEAGAAGKVPQPGSPADGGRDRRDDQPDDQPDDPRELEDAPAAA
                     TSPEAMVRTVFPGAQEVDR"
     gene            1582..2040
                     /locus_tag="VF954_01405"
     CDS             1582..2040
                     /locus_tag="VF954_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX9529764.1"
                     /translation="MTFEESGLGDLLRQAQAMQERLAEAQAAAADHEVQGHSGGGAVR
                     IVVTGDLEFRAVRIDPRAVDPGDVGMLEDLVLAALHDAVAQLNALGQQAVGSSLGGLD
                     LGGLDLGGFDLGGLDLEQLGDALGLPPGLEDLDGPSADDDGEGSDEPRHP"
     gene            2044..>2509
                     /gene="recR"
                     /locus_tag="VF954_01410"
     CDS             2044..>2509
                     /gene="recR"
                     /locus_tag="VF954_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX9529765.1"
                     /translation="MAGVYAGPVQDLIDELGRLPGIGPKSAQRIAFHLLKLSKEDALR
                     LARSIVEVKERISFCTRCFNVAEGELCGLCRDDRRDPTVLCVVEEPRDIVAVEKTQEF
                     RGRYHVLLGAINPIEGIGPDHLKVRELLARMEPEGITEVILCTNPNLEGEATA"
BASE COUNT          337 a          895 c          936 g          341 t
ORIGIN      
        1 ggtccagcag gggacgtcgc tcgacgtgat agagctcgac gccgcctcca acagcggcgt
       61 ggacgccatg cgcgacctcg tgtcccgggc cgccctcgcc acgcccggcc gctggaaggt
      121 ctacatcgtg gacgaggtgc acatgctgtc gagcgcggcg tccaacgcct tgctcaagac
      181 gctggaggag cctccgggcc acgtggtctt cgtgctggcc acgaccgacc cgcagaaggt
      241 gctgatgacc atccgcagcc ggacgcagca cttcgagttc cggctcttgg ggggagaaat
      301 cctgaccggg ttgctccgcg aggtccgtga tcgggcgggc ctcgaggtgc cgatggaggc
      361 cgtcgaggtc gcggtgcgca agggccacgg ctcggcgcgc gacgccctgt cggcgctgga
      421 ccaggtggct gcggccggcg aggtgcccga tgaggtcgcg gtgctcgacg aggtcgtgga
      481 tgccctcatc gagcgcgatc cgtcccgtgc cctggtagcc gtcgccgagg tcacggcgca
      541 gggccgggac gtgcagcggg tggcggtcga gctggtcgag cacctgcgaa atgtcttcct
      601 ggcgaccatg gccccggggc tggtgccgct ccccgacgac gctctggtgc acctccagga
      661 gcaggcccac cgcctcggca ccgctgccct ggtccgggcc atcgaggtcg tcggcgcgtc
      721 gcaggtcgac atgcgcgacg ccgtcgatcc gcgcatctgt ctcgaggtgg ccctggtccg
      781 actggcccgg ccggacgccg acgtgtccct ggccgccttg caggagcggc tggagcgagt
      841 ggagcagcgc gtgacgggcc acgtcgccga gccggcccgg acggcggcgc gccaagcggt
      901 cacggacccg tccgctcgga cccagccgcc ggcctcggca ccgaccccgg ccgggacccg
      961 ggccgatgcc gggtcggcac cctcgccgtc cggcgccgga ccgatgacgg gggccgcggc
     1021 cgcccggctg gccctgggtg gcgtccgcaa caaggcgggc gccgcgcccc ccgagatctc
     1081 gcgctcgggc ccacctccga cctcgggtcc gaggcccgcc cgccccgcag gggatgccat
     1141 agcccggggc gcccccggcg ggctgccctc ccgtgacgag ctgacgaagg tgtggggaga
     1201 cctcctgctc ggccagctgc cgccgcgcgc ccgggcccgg ttcgggcccg ggcggttcgt
     1261 cgaggtggac gaccgagcgg ctgtgttcgc cctgcccaac gatgttcacc gggcccggtg
     1321 cgaagaggtc cgtgccgagg tggagcaggt gctcagcgcc catttcgggg tgcccgttcc
     1381 gctcagcctg gtcaccgagg cgggcgcggc ggggaaggtg ccacagcctg gatcgcctgc
     1441 ggacgggggt cgggaccggc gagacgatca gccagacgat cagccagacg atccgcggga
     1501 gctcgaggac gcgccggccg cggcgacgtc ccccgaggcc atggtgcgca cggtgttccc
     1561 gggggcccag gaggtcgacc ggtgaccttc gaggagtccg ggctcggtga cctgctccgg
     1621 caggctcagg ccatgcagga gcgtctagcc gaggcccagg ccgcggccgc cgaccatgag
     1681 gtgcaaggcc actccggcgg cggcgcggtg cgcatcgtcg tgaccgggga cctggagttc
     1741 cgggcggtcc gcatcgatcc cagggcggtc gaccctggcg acgtcggcat gctcgaggat
     1801 ctcgtcctcg ccgcgctcca cgacgccgta gcccagctca acgccctcgg ccagcaggcc
     1861 gtcggcagct cgctcggagg cctcgatctc ggaggtctcg atctcggagg cttcgatctc
     1921 ggaggtctcg atctggagca gctcggcgac gccctcgggc tgccgccggg gctcgaggac
     1981 ctcgacggcc cgagcgccga cgacgacggg gagggctcgg acgagccccg gcatccctga
     2041 gtcgtggcgg gcgtctacgc cggtcccgtc caggacctga tcgacgagct cggccgcctt
     2101 ccgggcatcg ggcccaagtc ggcgcagcgc atcgcctttc acctgttgaa gctgtccaag
     2161 gaagatgcgc tccgcctggc ccggagcatc gtcgaggtca aggagcgcat ctcgttctgc
     2221 acccgctgct tcaacgtcgc cgagggcgag ctctgcggcc tgtgccgcga cgatcggcga
     2281 gacccgaccg tgctctgcgt ggtcgaggag cctcgcgaca tcgtggccgt ggagaagacg
     2341 caggagttcc ggggccggta ccacgtgctc ctgggagcca tcaaccccat cgaggggatc
     2401 gggcccgacc acctgaaggt gcgcgagctg ctggcccgga tggagcccga gggcataacc
     2461 gaggtgatcc tgtgcaccaa ccccaacctg gagggcgagg ccacggcga
//
LOCUS       DASWGG010000083         3874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1369622, whole genome shotgun sequence.
ACCESSION   DASWGG010000083 DASWGG010000000
VERSION     DASWGG010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369622"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VF954_01415"
     CDS             <1..585
                     /locus_tag="VF954_01415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9529766.1"
                     /translation="RLENEDGSLYPSARTFPDLVDALGHAALGLVAPANRFTRRYRML
                     DWDHGRAADVDWVSGACLLVRRDVFEALAGLDESYFMYLEDVDLCWRVWRAGWRVRYE
                     PDATVVHAQGVSTDQTPYRMLAAHHRSMHRFAVRTTEGWRRAALPAVSAGLVARLGVA
                     IAHRAVLGRWRRLPRPAVPGEAPAGRPGPAAANR"
     gene            597..1364
                     /locus_tag="VF954_01420"
     CDS             597..1364
                     /locus_tag="VF954_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529767.1"
                     /translation="MGKASSNKKVARAASTGGGRTNRGATPVSWYSIMGIIVLAGLAL
                     VAFSRQERLDLRGGPHPKGQDHWHAALGIDICGTFEKNLPQPAKLLGLHTHNDGLIHV
                     EPFVTAAPQDSGHHATLARFVEGIPGLKITSTQLQYPGQKLYKNGDKCGDKAGQVVIK
                     WWPDAFGTKSQTINAPSGLRIQNGGAVTVAFLPAGTDVPKPPKAVLDALTSTEANPSI
                     AEQGATPSSSPPVSTAPSAATTAPPTATTAPTATTKP"
     gene            1361..2437
                     /locus_tag="VF954_01425"
     CDS             1361..2437
                     /locus_tag="VF954_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX9529768.1"
                     /translation="MKAVILVGGEGTRLRPLTYDVPKPLLPVAAISIIERILGHLGGH
                     GVDHAVLSMGYRPDAFVAAFPENRAAGIRLSYAVEPEPLDTAGAIRFAATESGIDETF
                     LVVNGDVLADLDVGLLVAFHRASGAEGTVALTPVHDPSAFGVVATDDRGRVEAFIEKP
                     AAGEAPTNLINAGTYVLEPSVLGRMPEGRPANVERETFPAMVAAGTLYGHASDAYWTD
                     TGTAALYLQANLDCVAGRRGLPPAPGARAAGDGVWHLGRPVVEGQVAGPTLIADACFV
                     AGSAVVANSVLGPGCRVEAGARVRGSVVLPGTSVGPEACVDGSVVGEAAAIHRGAHVS
                     GLAVVGCRAEVPEGARVVGGRFPG"
     gene            2434..3399
                     /locus_tag="VF954_01430"
     CDS             2434..3399
                     /locus_tag="VF954_01430"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9529769.1"
                     /translation="MRALVTGGAGFIGSALVDRLLAEGHSVDAVDDLSSGSLANLGEA
                     RADPVFDFHFHRLDIRRPELVDLVVRRAPEVVFHLAAPPARRSGRADPRADADQHILG
                     SLNVMEAAVAAGATKVVFASGAAIYGAVEPEELPIRETHPQRPRSFAGVAKRAVGDYL
                     EAYRVHHELEFTSLALAHVYGPRQDPALGDGVVAVMAARLVAGLPSTIHGSGRHSRDF
                     VYVDDAVDALARAIGRGGGLLLNVGTGVATAIEDLYATVARAAGSDHPAAFGPARAGE
                     VDHVALDPGRAAIHLGWRPWTSLAAGVASTVDWLSGPSYRNRSSS"
     gene            complement(3375..>3874)
                     /gene="cofE"
                     /locus_tag="VF954_01435"
     CDS             complement(3375..>3874)
                     /gene="cofE"
                     /locus_tag="VF954_01435"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX9529770.1"
                     /translation="REAVRVLRRRGDLIVTETSHGFVCANAGIDLSNVERGFAALLPV
                     DPDRSARRIRDGLRARAGAEVGVIVSDTFGRAWRRGLTDVAIGCAGIGAVVDLRGSTD
                     ALGRELQVTEVCIADEIAGAAELVMGKSAGVPVVLVRGVHRSWLRDGSVRQEVVRRHD
                     EDLFR"
BASE COUNT          500 a         1471 c         1400 g          503 t
ORIGIN      
        1 cgactggaga acgaggacgg cagcctgtac ccctcggccc ggacctttcc cgacctggtc
       61 gacgccctgg gccacgccgc cctcgggctg gtggcacccg ccaatcgctt cacccggagg
      121 taccggatgc tcgactggga ccacggccgg gcggccgacg tcgactgggt gtcgggcgcg
      181 tgcctcctcg tgcggcgtga cgtcttcgag gccctggccg gcttagacga gtcgtacttc
      241 atgtacctcg aggacgtgga cctctgctgg cgggtctggc gggccggctg gcgcgtgcgc
      301 tacgaacccg acgccacggt tgtccacgcc cagggcgtct ccaccgacca gacgccctac
      361 cggatgctgg ccgcccatca ccgctcgatg caccgcttcg ccgtcaggac caccgagggc
      421 tggcgccggg cggctctgcc ggcggtctcc gccggcctgg tcgcacggtt gggcgtggcg
      481 atcgcccacc gggccgtgct gggccgctgg aggaggctgc ccaggccggc ggtccccggc
      541 gaggcgcctg cggggcggcc gggccccgcc gcggcgaaca ggtagcgtga cggcccatgg
      601 gcaaggcgtc atcgaacaag aaggtcgcgc gcgccgccag caccggaggc ggccgcacca
      661 accggggcgc caccccggtg agctggtact cgatcatggg gatcatcgtg ctggcgggcc
      721 tcgccctggt ggccttcagc cggcaggagc ggctcgatct gcggggaggc ccgcatccca
      781 aaggccagga ccactggcat gccgccctcg gcatcgacat ctgcgggacg ttcgagaaga
      841 acctccccca gcccgccaag ttgctggggc tgcacaccca caacgacggc ctgatccacg
      901 tggagccctt cgtcaccgcg gcgccccagg actccggcca ccacgccacc ctggcccgct
      961 tcgtcgaggg catccccggc ctgaagatca cctcgacgca gctccagtac cccggccaga
     1021 agctctacaa gaacggcgac aagtgcggcg acaaggctgg ccaggtcgtc atcaagtggt
     1081 ggccggacgc cttcggcacc aagagccaga ccatcaatgc cccctcgggc ctgcgcatcc
     1141 agaacggcgg ggccgtgacc gtcgccttcc tccccgccgg cacggacgtg cccaagccgc
     1201 ccaaggcggt gctcgacgcc ctgacctcga ccgaggccaa cccgtcgatc gccgagcaag
     1261 gcgccacacc ctcgagctcg ccgccggtct ccaccgcccc gtcggcggcc acgacggccc
     1321 cacccacggc caccactgcg cccacggcca ccaccaagcc gtgaaggccg tgatcctggt
     1381 cgggggcgag ggcactcgcc tccggcccct gacctacgac gtccccaagc cgctcctgcc
     1441 cgtcgccgcc atcagcatca tcgagcggat cctcggccac ctcggtggac acggcgtgga
     1501 ccacgcggtg ctgtccatgg gctaccggcc ggacgcgttc gtggccgcct tcccggagaa
     1561 ccgggcggcc ggcatccgcc tcagctacgc ggtcgagccc gagccgctcg acaccgcggg
     1621 cgccatccgg ttcgcggcca cggagtcggg catcgacgag acgttcctgg tcgtcaacgg
     1681 cgacgtgctg gccgacctgg acgtcgggct gctggtcgcc ttccaccggg cgagcggggc
     1741 cgagggcacc gtcgccctga cgccggtgca cgacccgtcc gccttcgggg tggtggcgac
     1801 cgacgaccgg ggcagggtgg aggccttcat cgagaagccg gccgccggcg aggcgcccac
     1861 caacctcatc aacgccggga cctacgtgct cgagccgtcg gtgctcgggc gcatgccgga
     1921 gggacggccc gccaacgtcg agcgggagac ctttcccgcc atggtcgcgg cgggcacgct
     1981 gtacggccac gcgtcggacg cctactggac cgacaccggc accgcggcgc tctatctcca
     2041 ggccaacctg gactgcgtgg cggggcggcg gggcctgccg cccgcgccgg gcgcgcgggc
     2101 cgcgggcgac ggcgtgtggc acctcggccg gccggtggtc gagggtcagg tggccggccc
     2161 gacgctgatc gccgacgcct gcttcgtggc cggatcggcg gtggtggcca acagcgtgct
     2221 cggccccggc tgccgggtcg aggccggcgc ccgggtccgg ggctcggtgg tgctgccggg
     2281 gacctcggtc gggccggaag cctgtgtgga cggctccgtc gtcggcgagg ccgcggccat
     2341 ccaccgcggc gcccacgtgt ccgggctggc ggtcgtcggc tgccgggccg aggtgcccga
     2401 gggggcccgg gtggtcggag gccggttccc cgggtgagag cgttggtcac ggggggcgcc
     2461 ggcttcatcg gctcggcgct tgtcgaccgg ctgctggccg agggccactc ggtggacgcc
     2521 gtcgacgacc tgtcgtccgg ttcgctggcc aacctgggag aggcgcgggc cgatccggtc
     2581 ttcgacttcc attttcaccg gctcgacatc cgccgcccgg agctggtgga cctcgtcgtg
     2641 cggcgggcgc ccgaggtggt cttccatctc gccgcaccgc ctgccaggcg gtccggccgg
     2701 gccgatcccc gggccgacgc cgaccagcac atactgggca gcctgaacgt catggaggcc
     2761 gcggtcgccg ccggcgcgac caaggtggtg ttcgcgtcgg gcgccgccat ctacggcgct
     2821 gtcgagcccg aggagctgcc catccgcgag acgcacccgc aacggccccg gtcgttcgcg
     2881 ggcgtggcca agcgggcggt cggggactac ctcgaggcct accgggtgca ccacgagctg
     2941 gagttcacca gcctggcgct ggcccacgtc tacgggccgc gccaggaccc cgccctcggc
     3001 gacggcgtgg tggccgtgat ggcggcccgg ctcgtcgcgg gcttgccgtc caccatccat
     3061 ggcagcgggc gccacagccg ggacttcgtg tacgtggacg acgcggtcga cgccctggcc
     3121 cgggcgatcg gccggggcgg cgggctgctg ctcaacgtcg gcaccggcgt ggccacggcg
     3181 atcgaggacc tgtacgccac cgtggcccgg gctgcgggat cggaccaccc ggccgccttc
     3241 gggccggccc gggccggcga ggtggaccac gtcgcgctgg atccgggccg ggccgccatc
     3301 cacctcgggt ggaggccgtg gacgtcattg gccgcgggcg tggcctcgac cgtcgactgg
     3361 ctgtccggcc cgtcctaccg gaacaggtcc tcgtcgtgac gccgcacgac ctcctggcgg
     3421 accgagccgt ctcgcagcca cgaccggtgc acgccccgga cgaggacgac gggcaccccg
     3481 gccgacttgc ccatgacgag ctcggccgcg ccggcgatct cgtcggcgat gcagacctcg
     3541 gtgacctgca gctcccggcc gagggcgtcg gtgctgcccc gcaggtccac gaccgcaccg
     3601 atgccggcgc agccgatggc gacgtcggtc agcccgcgcc gccacgcccg cccgaacgtg
     3661 tcggacacga tcaccccgac ctccgcgccg gctctggccc gcagcccgtc ccggatgcgg
     3721 cgggcggagc ggtcggggtc gaccggcagc agggcggcga acccgcgctc gacgttggag
     3781 agatcgatcc ccgcgttggc gcacacgaag ccgtgactgg tctcggtcac gatcagatct
     3841 ccccggcggc gcaggacacg cacggcctct cgtt
//
LOCUS       DASWGG010000084         5102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2985132, whole genome shotgun sequence.
ACCESSION   DASWGG010000084 DASWGG010000000
VERSION     DASWGG010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2985132"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VF954_01440"
     CDS             <1..395
                     /locus_tag="VF954_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9529771.1"
                     /translation="AMAAPFFETAEVIELHHDDKIDAPSGTALLTAARMDEARRAAGR
                     RWGEDPTQTVALPGARGGSSDGGVHVHSVRLRGLVAHQEVLLGTTGQTLSIRHDTTDR
                     TSFMPGVLMALKAVAGRPGMTVGLDALL"
     gene            444..2066
                     /locus_tag="VF954_01445"
     CDS             444..2066
                     /locus_tag="VF954_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX9529772.1"
                     /translation="MQGLMQECPLTLPMMFRRAERLFPGKRIVTATAGAPEETTYGAW
                     AARARRLGGALDALGISPDGRVGTFAWNTARHLELYFAAPCTGRVLHTLNIRLFADQL
                     TYIANHAEDEVVFVDRSLIGQLWPVAQGFDTVRRFVVMDDGQGDVPDDERIVDYEELL
                     ASAEPVEFRVDDEDRAAAMCYTSGTTGNPKGVVYSHRSTVLHSLGTMMVDSVGVCEAD
                     VVLPVVPMFHANAWGLCQAAVMAGADLVLPGADLSPPAIAELLETRRVTLAAGVPTIW
                     MGVLPLLEGRDTSALTRVLCGGSAVPRALSEAYRQQIGRPILQAWGMTETSPLASVAR
                     IRSTIAGRTEDQLAEVRATQGVPVAGVEARIVEPDTSDELPWDGESRGELQVTGPWVA
                     RSYYDDPRSGESFTVDGWLRTGDVATISPEGYIRLVDRTKDVIKSGGEWISSVDLENE
                     LMAHPQVAEAAVIAVPHEKWQERPLACVVVKPGGELTKAEVIDFLTPRVARWWLPDDV
                     VFIDEVPKTSVGKFSKKTLRDRYAGHNLPTSP"
     gene            2142..3131
                     /locus_tag="VF954_01450"
     CDS             2142..3131
                     /locus_tag="VF954_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529773.1"
                     /translation="MDKRQRVLIGAAAGGAGLLAAGAVAVAVMAGRGGTSRVRTTATT
                     APATTSTTDLTETTLALEPVDSPTTNAPPQSSTTTATTALAAALSGQGAVLIPSSAAG
                     RLLPDGSGCSALGDGAGWTVQCGQLGPSAIWLTETRPAAAGSTATRAYVLRPADPATN
                     STVASGHAGAAPTTTATTTKSTTATTTSAPTATTSPGGTLWAVALAEQDDAGGRFGGL
                     DAASGGVRGDGSQAVVFGFRQPGAAGVLSLDLVEVPPATTRPSVSVHMDLTRGSVRLS
                     TGELDLWAASYVPGDATCCPSAFVHQVIQYQADAWRVVAASRISPANVPPSQV"
     gene            3140..3718
                     /locus_tag="VF954_01455"
     CDS             3140..3718
                     /locus_tag="VF954_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9529774.1"
                     /translation="MPLTVRDDARERILAGAYACVARYGLAKTTVDDVARLSGLSRAT
                     FYRYFPGGKDQLQREVVAWEVRRFFARLAEAVGPAPDLATMLVEGLLYAHRALIDHEV
                     LQKVLQTEPELLLPLLSVEANRLLPLVAGFVEPYVARAPLLPGLEPDQAAGYLARMIL
                     SYIEAQGRWDLTDRSEVERLVRVELLGGIVTG"
     gene            3791..4441
                     /locus_tag="VF954_01460"
     CDS             3791..4441
                     /locus_tag="VF954_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9529775.1"
                     /translation="MVVATAVNVDLGLAGPAVSPPAIRMVDATLRCIARWGVAKTTLD
                     DVAREAGCSRATVYRLFPGGKDGLLDAVARTETERFFAGLAEHMAGAESLEELVVAGV
                     GEAGRRLVGHPALRFLAACEPDALLPLLSFAQKDRVLGFASSWAEPWLRPWLGDHAGR
                     AVEWIARLVLSYAACPADGIDLSDGESVRRLVTTFVLPGLARLAEDTSAPTSRGSQ"
     gene            4438..>5102
                     /locus_tag="VF954_01465"
     CDS             4438..>5102
                     /locus_tag="VF954_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX9529776.1"
                     /translation="MTATNQDLIGRSEINDIEAILSVTNTDMTSTIHAVEDNADAIFT
                     WDYEKGARPKLNRLYEKAKVSMWNGETDLPWDTEVDQEAVVVANAAAAGGIDAGMDLA
                     GTAFEKWTEKDWIRFGIESQNWMLSQFMHGEQGALVCTAKIVETVPWIDAKYYASTQV
                     MDEARHVEVFARYLDTKLSGHYPINAHLQMLLDDIINDSRWDMTYLGMQIMVEGLALA
                     AFG"
BASE COUNT          732 a         1859 c         1809 g          702 t
ORIGIN      
        1 cggcgatggc cgcccccttc ttcgagacgg ccgaggtcat cgagctgcac cacgacgaca
       61 agatcgacgc cccctcgggc acggcgctgc tgaccgcggc ccgcatggac gaggccaggc
      121 gggcggcggg ccggcggtgg ggagaggacc cgacgcagac cgtggccctg cccggggcac
      181 ggggcggctc gtccgacggc ggggtgcacg tccactccgt gcgcctccgc gggttggtgg
      241 cccaccaaga ggtgctgctg ggcacgaccg gccagacgct ctcgatccgc cacgacacga
      301 ccgaccgcac gagcttcatg cccggcgtcc tcatggccct gaaagcggtc gcaggccggc
      361 ccggcatgac cgtgggcctc gacgcgctcc tctgaggccg cacctccggc taggttgcgc
      421 acgtcatctg tgaagggggg cccatgcagg ggctcatgca ggaatgcccg ctgacgttgc
      481 ccatgatgtt ccgccgggcc gagcgtctct tcccgggcaa gcgcatcgtg accgcgaccg
      541 cgggagcccc agaggagacc acctacggcg cgtgggcggc gcgggcccgg cgcctcggcg
      601 gggccctcga cgccctcggc atctcgcccg acgggcgggt cggcaccttc gcctggaaca
      661 cggcccgcca cctcgagctg tacttcgcgg cgccctgcac cggcagggtg ctgcacaccc
      721 tcaacatccg gctcttcgcc gaccagctga cctacatcgc caaccacgcc gaggacgagg
      781 tcgtcttcgt cgaccgctcg ctcatcgggc agctgtggcc ggtggcccag ggcttcgaca
      841 ccgtccggcg attcgtcgtc atggacgacg gccagggaga cgtgcccgac gacgagcgga
      901 tcgtggacta cgaggagctg ctggcctccg ccgagccggt ggagttcagg gtcgacgacg
      961 aggaccgggc cgccgccatg tgctacacga gcggcaccac cggcaacccg aaaggggtgg
     1021 tgtactcgca ccgatcgacc gtgctgcact ccctcggcac gatgatggtc gacagcgtcg
     1081 gcgtgtgcga ggcggacgtc gtcctgccgg tggttccgat gttccatgcc aacgcgtggg
     1141 ggctgtgcca ggcggcggtc atggcgggag cggacctcgt cctgccgggg gccgacctgt
     1201 cgccgccggc gatcgccgag ctcctcgaga cgcgccgcgt gaccctggcc gcgggcgtcc
     1261 cgaccatctg gatgggcgtg ctgcctttgc tcgaggggcg cgacacgtcg gcgctcaccc
     1321 gggtcctctg cggcgggtcc gcggtgccgc gcgccctctc cgaggcctac cggcagcaga
     1381 tcggccgacc catcctccag gcgtggggca tgaccgagac cagcccgctg gcgtcggtgg
     1441 cccgcatccg cagcacgatc gcgggccgga ccgaggacca gctcgccgag gtgcgggcca
     1501 cacagggggt gccggtagcc ggcgtggaag cccgcatcgt cgagcccgac accagcgacg
     1561 agctgccatg ggacggtgag tcccgcggcg agctccaggt caccgggccg tgggtggcac
     1621 ggagctacta cgacgacccg cgctccggtg agtcgttcac cgtggacggc tggctgcgga
     1681 cgggcgacgt ggccaccatc tcgcccgagg gctacatccg actggtcgac cggacgaagg
     1741 acgtcatcaa gtcgggcggg gagtggatct cttcggtgga cctcgagaac gagctcatgg
     1801 cccaccctca ggtcgccgag gccgcggtga tcgccgtccc ccacgagaag tggcaggagc
     1861 gcccgctcgc ctgcgtcgtc gtcaagcccg gcggggagct gaccaaggcg gaggtgatcg
     1921 atttcctcac gcctcgggtc gcgaggtggt ggctgcccga cgacgtggtc ttcatcgacg
     1981 aggtgcccaa gacatcggtc gggaagttct ccaagaagac cctccgggac cgatacgccg
     2041 gacataacct gccgacttcg ccctgagggc gcgccgccgg ggtggctcgg gcggcgcgcc
     2101 tgccaccgtg gggccggccg gcgccggtac cctgcggcag cgtggacaag cgccagcggg
     2161 tgctgatcgg tgcggctgcg ggcggggccg ggctgctggc ggccggcgcc gtggccgtgg
     2221 ccgtgatggc cggccggggg ggcacgagcc gcgtgcgcac caccgcgacc accgcgccgg
     2281 cgacgacctc caccacggac ctcacggaga ccacgctcgc cctggagccg gtcgacagcc
     2341 cgacgacgaa cgccccgccc cagtcgtcga ccacgaccgc tacgacggcg ctggccgccg
     2401 cgctgagcgg ccagggcgcg gtgctcattc cctcctccgc ggccggccgg ctcctgccag
     2461 acggctcggg ctgctcggcg ctgggcgacg gggccggctg gacggtccag tgcggccagc
     2521 tggggccgag cgccatctgg ttgaccgaga cccgcccggc tgcggcaggc tcgactgcga
     2581 cccgcgccta cgtcttgcgg ccggccgacc ctgcgaccaa cagcaccgtc gcgtccggcc
     2641 acgccggggc ggcgccgacg acgaccgcca cgactacgaa gtcgaccacc gcgaccacca
     2701 cctccgcgcc caccgcgacg accagcccgg gcgggacgct gtgggccgtg gctctggccg
     2761 agcaggacga cgccgggggc cggttcggcg ggctggacgc cgcctcgggc ggggtgcgcg
     2821 gcgacggcag ccaggccgtc gtcttcggct tccgccagcc gggcgcggcc ggcgtcctca
     2881 gcctcgacct ggtcgaggtc ccacccgcga cgacccggcc gtcggtcagc gtccacatgg
     2941 acctcaccag gggttcggtc cgcctgtcga ccggcgagct ggacctgtgg gcggcgtcgt
     3001 acgtgcccgg cgacgccacc tgctgtccgt cggccttcgt ccaccaggtg atccagtacc
     3061 aggccgacgc gtggcgggtc gtggccgcca gccgcatctc gccggccaac gtgcccccga
     3121 gccaggtcta gccctcaccg tgcccctcac tgtgcgcgac gacgcccggg agcgcatcct
     3181 cgccggcgcc tatgcctgcg tggctcgcta cgggttggcc aagacgactg tcgacgacgt
     3241 cgcccgcctc tccggcctgt cgcgcgccac cttctaccgc tacttcccgg ggggcaagga
     3301 ccagcttcag cgggaggtgg tggcatggga ggtccgccga ttcttcgccc gcctggcgga
     3361 ggcggtgggg cccgcccccg acctggcgac catgctcgtc gaggggctgc tctacgccca
     3421 tcgtgcgctc atcgaccacg aggtcctcca gaaggtcctc cagaccgagc cggagctcct
     3481 cctgccgctg ctcagcgtgg aggccaaccg gctcctgccc ctcgtggccg gcttcgtcga
     3541 gccctacgtc gcccgcgccc ccctcctgcc ggggctggag cccgaccagg ccgccggtta
     3601 cctggcccgg atgatcctga gctacatcga ggcgcagggc cggtgggacc tgaccgaccg
     3661 gtccgaggtg gagcgcctgg tccgcgtcga gctcctcggc ggcatcgtca ccggatgacc
     3721 gggcgtatga caggtgtcac aatgagacgc cattgacgat gagacaaaga tcggcctatc
     3781 gtctcaacac atggtggtcg cgactgcggt gaacgtcgat ctgggcctgg cagggccggc
     3841 ggtctcgccg ccggccatcc ggatggtgga cgccaccctg cggtgcatcg cccgctgggg
     3901 ggtcgccaag accacgctcg acgacgtggc ccgcgaggcc ggctgcagcc gggccacggt
     3961 ctaccggctg ttcccgggcg gcaaggacgg cctgctcgac gcggtggccc ggaccgagac
     4021 ggagcgcttc ttcgccggcc tggccgagca catggccggc gccgagagcc tcgaggagct
     4081 ggtggtggcc ggggtcgggg aggccggtcg gcggctggtg ggccaccccg ccctgcggtt
     4141 cctggccgcc tgcgagcccg acgctctcct tcccttgctg agcttcgccc agaaggatcg
     4201 ggtgctcgga ttcgccagca gctgggccga gccgtggctg cgcccctggc tgggcgacca
     4261 cgccggccgg gccgtggagt ggatcgcccg cctcgtgctg tcctacgccg cctgcccggc
     4321 cgacggcatc gacctgagcg acggggagtc cgttcgccgg ctcgtcacca ccttcgtgct
     4381 ccctggcctg gcccgcctgg ccgaagacac gtcagcaccc acatcaaggg gaagccaatg
     4441 actgccacca accaagacct gatcggacgg tccgagatca acgacatcga ggccatcctc
     4501 tcggtgacca acacggacat gacctcgacc atccatgcgg tcgaggacaa cgccgacgcc
     4561 atcttcacct gggactacga gaagggtgcc cgccccaagc tcaaccgcct gtacgagaag
     4621 gccaaggtct cgatgtggaa cggtgagacc gacctgccgt gggacaccga ggtcgaccag
     4681 gaggcggtgg tggtggccaa cgcggccgct gccggcggga tcgacgcggg catggacctc
     4741 gccggcacgg ccttcgagaa gtggaccgag aaggactgga tccggttcgg gatcgagagc
     4801 cagaactgga tgctctcgca gttcatgcac ggcgagcagg gggcgctcgt gtgcacggcc
     4861 aagatcgtcg agaccgtccc gtggatcgac gccaagtact acgcgtcgac ccaggtcatg
     4921 gacgaggccc gccacgtcga ggtcttcgcc cggtacctcg acaccaagct gtcgggccac
     4981 tacccgatca atgcccacct ccagatgctg ctcgacgaca tcatcaacga cagccgctgg
     5041 gacatgacct acctgggaat gcagatcatg gtcgagggcc tggccctggc cgcgttcggc
     5101 tt
//
LOCUS       DASWGG010000085         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1052753, whole genome shotgun sequence.
ACCESSION   DASWGG010000085 DASWGG010000000
VERSION     DASWGG010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052753"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2926
                     /gene="mfd"
                     /locus_tag="VF954_01470"
     CDS             <1..2926
                     /gene="mfd"
                     /locus_tag="VF954_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX9529777.1"
                     /translation="VDLWPSTAGAPVRVDLWGDEVDRLTEFDPTDQRSSADVGHAEIF
                     PCRELLATDEVRRRAGRLVGDEPWGRGQWERLAEGLVFDGMESWLPWLTEGEHVLLDL
                     VGPDAQILLLEPRRMRDRAVEILDEEASLATTLAQTWGAAAEGRSFPSLHLPFDRLLA
                     HTRAPVWSVTPVPEGPATPRLDAHGWDPVVGDGERLVKQLRDLLSDRYRITICAEGAG
                     SGARVASVLRDQGLTVPFHEGGEEVDLAQPGVRVIVAPLERGFIVPGLRLAVLTEGDV
                     TGRRRAHRRARPRTRVTEGFFDSLRAGDYVVHHQHGVGRYRGMVTRAIGGAERDYLLL
                     EYKGGDKLYVPSDQIDAIQPYSGGEAPALHRLGGSDWQRTKAKVRAAVREIAQELVVL
                     YRRRVTSPGHAFGPDTPWQHEMEEAFPYVETPDQLKAIVEVKADMQEPVPMDRLVCGD
                     VGFGKTEVALRSVFKAIQDGKQAAVLVPTTLLAQQHYQTFSDRYAGYPVRVEVLSRFL
                     TGPQARKVVEGLALGTVDVVIGTHRLLSGDVRFKDLGLLVVDEEQRFGVTHKEAIKQL
                     STTVDVLTLTATPIPRTLEMSLTGIRDLTLLNTPPAERQPILTYVGEYDELAVTEAVR
                     RELLREGQVFFVHNRVADIERVAAELRTLVPEARIAVAHGQMDEGTLERVVLDFWEGA
                     YDVLVCTTIIESGIDMPTVNTLVVDRADVLGLGQLHQLRGRVGRAGMRAYAYLFFPPD
                     RSLSEEAYERLRTIGEHTELGSGFKIAMRDLEIRGAGNLLGEDQTGHIAAVGYDLYVR
                     MVSEAVAELKGEELREPAEIKLDLPIEAHLPKEYVARDDLRLEAYRRLALVTTRAEVQ
                     DIRSEWLDRFGPVPAPGEALLRVAALRADCARTGVREITVVRARPGGLGGGDTARISP
                     LALKTSAQIRLKRLQPKAVYKQDSQQLIVTLARGADPAEAVAVLLDELVPVQPAVSGA
                     AP"
     gene            2974..>3288
                     /locus_tag="VF954_01475"
     CDS             2974..>3288
                     /locus_tag="VF954_01475"
                     /inference="COORDINATES: protein motif:HMM:NF038012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9529778.1"
                     /translation="MHHLYLPLSVVALSLGAALCFALSSVLQHRAAVGVPDEHSLRPR
                     MFLRLGRDWRFYAGNGFDGLGFALQFLALRRGSLALVTPLLVTGLLFALPLGALLAKR
                     RLR"
BASE COUNT          428 a         1230 c         1167 g          463 t
ORIGIN      
        1 cgtggacctc tggccgtcca cggccggcgc ccccgtccgc gtcgacctgt ggggcgacga
       61 ggtcgaccgg ctgaccgagt tcgacccgac cgaccagcgc tcttcggcgg acgtcggtca
      121 cgccgagatc ttcccgtgcc gggagctgct ggcgaccgac gaggttcgcc ggcgggccgg
      181 ccggctggtc ggcgacgagc cctggggccg gggccagtgg gagcgcctgg ccgaggggct
      241 cgtcttcgac ggcatggagt cttggttgcc gtggctgacc gagggcgagc acgtgctgct
      301 cgacctggtc ggcccggacg cccagatcct cctgctggag ccgcgccgta tgcgcgaccg
      361 ggcggtggag atcctcgacg aggaggcgtc gctggccacc acgctggccc agacgtgggg
      421 cgcggccgca gaaggccggt ccttcccgag cctccacctg cccttcgacc ggttgctggc
      481 ccatacccgg gcccccgtct ggtcggtgac gccggtgccg gagggaccgg ccaccccccg
      541 cctcgacgcc cacggctggg accccgtggt cggcgacggc gagcggctgg tgaagcagct
      601 ccgggacctg ctctccgacc gctaccggat caccatctgc gccgaggggg ccggcagcgg
      661 ggcgcgcgtc gcctcggtgc tccgggacca gggcctgacc gtgccgttcc acgagggggg
      721 cgaggaggtc gacctggccc agcccggggt ccgggtcatc gtcgcccccc tggagcgggg
      781 cttcatcgtc ccgggcctgc gcctggcggt gctgaccgag ggcgacgtca ccggccggcg
      841 ccgggcgcat cgccgggccc ggccccgcac gcgggtgacc gagggcttct tcgactcgct
      901 ccgggccggc gactacgtgg tgcaccacca gcacggcgtg gggcgctacc gcggcatggt
      961 cacgcgggcc atcggaggcg ccgaacgcga ctacctgctg ctcgagtaca agggcgggga
     1021 caagctctac gtcccgtccg accagatcga cgccatccag ccctacagcg ggggcgaggc
     1081 gccagccctg caccgcctgg gcgggtccga ctggcagcgg accaaggcca aggtccgtgc
     1141 cgccgtgcgg gagatcgccc aggagctggt cgtgctgtac cgcaggcggg tcacctcgcc
     1201 cggccatgcc ttcggcccgg acaccccgtg gcagcacgag atggaggagg ccttcccgta
     1261 cgtcgagacg cccgaccagc tcaaggccat cgtcgaggtg aaggcggaca tgcaggagcc
     1321 tgtcccgatg gaccgcctgg tgtgcggcga cgtcggcttc ggcaagaccg aggtggcgct
     1381 gcggtcggtc ttcaaggcca tccaggacgg caagcaggcc gcggtgctcg tccccaccac
     1441 cctgctcgcc cagcagcact accagacctt ttcggaccgc tacgccggct accccgtgcg
     1501 ggtggaggtc ctgtcccgct tcctgaccgg cccgcaggcc aggaaggtgg tggagggcct
     1561 ggccctcggc acggtcgacg tcgtcatcgg gacccaccgg ctgctgtcgg gcgacgtgcg
     1621 gttcaaggac ctggggctgc tggtcgtcga cgaggagcag cgcttcggtg tcacccacaa
     1681 ggaggccatc aagcagctca gcaccaccgt cgacgtgctc accctgacgg ccaccccgat
     1741 cccgcggacc ctggagatga gcctgaccgg catccgggac ctgaccctgc tcaacacccc
     1801 acccgccgaa cgccagccca tcctcaccta cgtcggtgag tacgacgagc tcgccgtgac
     1861 cgaggccgtc cggcgcgagc tgctccgcga gggccaggtc ttcttcgtcc acaaccgggt
     1921 agccgacatc gagcgggtgg ccgccgagct ccgcacgctg gtcccggagg cccgcatcgc
     1981 cgtggcccac ggccagatgg acgagggcac cctggagcgc gtggtgctcg acttctggga
     2041 gggcgcctac gacgtgctgg tgtgcacgac gatcatcgag tccggcatcg acatgcccac
     2101 ggtcaacacc ctggtcgtcg atcgggccga cgtgctgggc ctgggccagc tccaccagct
     2161 ccgcggccgg gtgggacggg cggggatgcg ggcctacgcc tacctcttct tcccgcccga
     2221 ccggtcactg tcggaggagg cctacgagcg gctccgcacc ataggcgagc acaccgagct
     2281 cggctcgggc ttcaagatcg ccatgcgcga cctcgagatc cgcggcgcgg gcaacctgct
     2341 cggtgaggac cagaccggcc acatcgccgc cgtgggctac gacctgtacg ttcgcatggt
     2401 gagcgaggcc gtggccgagc tcaagggcga ggagctgcgg gagccggccg agatcaagct
     2461 cgaccttccc atcgaggccc acctgcccaa ggagtacgtg gcgcgcgacg atctccggct
     2521 ggaggcctac cgccggctcg ctctcgtcac gaccagggcc gaggtgcagg acatccgcag
     2581 cgagtggctc gaccgcttcg gccccgtgcc tgcgcctggc gaggccctgc tccgcgtcgc
     2641 cgccctgcgg gcggactgcg cccggaccgg cgtgcgggag atcaccgtgg tcagggcccg
     2701 tccgggcggc ttgggcggcg gggacaccgc tcgcatctca ccgctcgccc tcaagaccag
     2761 cgcgcagatc cggctcaagc gccttcagcc caaggccgtc tacaagcagg acagccagca
     2821 gctgatcgtg acgctggcgc ggggcgccga cccggccgaa gcggtggcgg tgctgctcga
     2881 cgagctcgtg cccgtccaac ccgccgtctc cggggcggcg ccctgagcgc gccggccgcg
     2941 gcccgcccgc tgcggggccg ggccttcccc gggatgcatc acctgtacct gccgctgtcg
     3001 gtcgtcgccc tctccctggg tgccgccctc tgcttcgccc tctcgtccgt tctccagcac
     3061 cgggccgcgg tgggcgtgcc ggacgagcac tccctgcgcc cgcgcatgtt cctgcgcctg
     3121 gggcgcgact ggcggttcta cgccggcaac ggcttcgacg ggctgggctt cgccctccag
     3181 ttcctcgccc tgcggcgggg ctcgctcgcc ctggtgaccc cgttgctcgt gaccggcctg
     3241 ctcttcgccc tcccgctcgg cgccctgctg gccaagcgcc ggctgcgc
//
LOCUS       DASWGG010000086         6064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1697699, whole genome shotgun sequence.
ACCESSION   DASWGG010000086 DASWGG010000000
VERSION     DASWGG010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697699"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VF954_01480"
     CDS             complement(<1..248)
                     /locus_tag="VF954_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9529779.1"
                     /translation="MTLFDDVVGQPRAVAELRAAAAAPVHAYLLVGPPGAGKRTAARS
                     FAAALLCGSGGCGSCEICRRVLSGVHPDYVLVERVGPS"
     gene            complement(250..858)
                     /gene="tmk"
                     /locus_tag="VF954_01485"
     CDS             complement(250..858)
                     /gene="tmk"
                     /locus_tag="VF954_01485"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX9529780.1"
                     /translation="MSFIVIEGGEGSGKSTQAARLARALGAVLTREPGGTQVGEQIRA
                     VLLDPESGALDPRAEALLMAAARAQHVVEVIRPALAAGRTVVCDRYAGSSYAYQGYGR
                     GLALSDLRCLSGWAADGLEPDLVILLDVPAEVAASRIAGQAEDRLESERGGFHDRVRL
                     GFRQLAGADPDRWAVVDGTGPVEEVAARVGQAHDRWSGGSGR"
     gene            complement(855..2273)
                     /locus_tag="VF954_01490"
     CDS             complement(855..2273)
                     /locus_tag="VF954_01490"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9529781.1"
                     /translation="MADSSTGGLRAARDGVGPTSAPGHPLGSPMFARLWCSQAVSSLG
                     DWLGLVAITALAYRLGAGSDGAAISLVLIARLVPGLFLASFAGVLVDRWDRRRVMVAC
                     DVGRGTVLALLPFATNVGELVAASFVLEMLTLMWTPAKEASVPNLVPPSFLPNANSLS
                     LAAAYGTFPVAAALFAALAAAAPALGVRQESLAMWADVATFFLSAAVISTLSLPRPKP
                     QESGDRGFDLGRTIVELREGWQFVVDSPVVRAVIIGLGTGMIGGAMIVPLGPIMAKQV
                     FGAGPAGFGVLLASLGVGVAGGIVGLGLVQHRVDHVRVFLAALLSAGPCLLLGAAMSH
                     LGPACFCVGALGLCAGAVYVLGFSLLQANVADELRGRTFATLYATVRICVLLALTVAP
                     VLSGVLDVLSTATVRGQLHLGPATLSVPGVRLTLWLAGLIIVGAGLLARRTLGSPVVA
                     GAVVAGPVVAGPVVAAPAVEGP"
     gene            complement(2330..4981)
                     /gene="topA"
                     /locus_tag="VF954_01495"
     CDS             complement(2330..4981)
                     /gene="topA"
                     /locus_tag="VF954_01495"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX9529782.1"
                     /translation="MPKPLVIVESPSKAKTIAGFLGRDFVVESSIGHIRDLPRNAADV
                     PAAYKREAWARLGVDTANDFKPLYVVAPEKKSQVAKLKALLKDASELYLATDEDREGE
                     SIAWHLLEVLNPRVPVKRMVFHEITRPAIEQAVDDWRELDRHLVDAQEARRILDRLYG
                     YEVSPVLWKKVMPRLSAGRVQSVATRILVDRERARMRFRSAGWWDIEGTFDAPTSPPS
                     FTAGLVAVDGTRVATGRDFGEDGALRANAAVVRLDESAARSLADRLAGSTLSVRSVEE
                     RPFRRSPHPPFMTSTLQQEAGRKLRFPSQRTMSIAQRLYENGFITYMRTDSTTLSSTA
                     MDAARSQARALYGPDYVPDSPRRYEKKVKNAQEAHEAIRPAGEVFRMPDEAGLNGDEA
                     RLYELIWKRTVASQMADARGQSAQVRLGGVAPDGGEDVELAASGKVITFPGFMRAYVE
                     GADDPDAELEDQEVRLPAMRQGDAVSTVAVEAKTHTTQPPARFTEASLVKALEELGVG
                     RPSTYATILGTIQDRGYVWKKGTALVPSFIAFAVTALLEAHFGALVDYGFTASMEGVL
                     DEIAGGQAEVVPWLRTFYFGNGLPGLKRLVSEHLDDIDAREINSIPIGVDAEGRDLVV
                     RVGRYGPYVQRGDQRASIPDDVAPDELTAERVEQLLVAPAGDRALGTDPASGLPVVVK
                     AGRYGPYVQLGDAEPGSKTRPATASLFKAMDPGTVTLDEGLRLLSLPRVVGAAPDGQE
                     IVAQNGRYGPYLTKGSDTRTMAAEEDLFTVTVEQALELFAQPKRGRGRQDAAPMRELG
                     PDPATGRQMVLRSGRFGPYVTDGESNASLPRGDSPEELTSERAAELLARRREMGPPAK
                     RARPAKKVAGAKKAGGAKKARPRRPGG"
     gene            5086..5700
                     /locus_tag="VF954_01500"
     CDS             5086..5700
                     /locus_tag="VF954_01500"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX9529783.1"
                     /translation="MHALDPTHLLDTYGIWGIYAVIFAETGLLIGFFLPGDSLLVTAG
                     LLASTGKPGDVHLNLAALLVGCAVAAVAGAQAGFVIGRRVGPALFRRPDSRIFKQRYV
                     ERTHETLARYGAVKAIVLARFIPVVRTFLNPVAGVGGVDTGLFTAANLGGGVAWTTSM
                     VLLGYTVGSTVKGIDRYLVPAIIVISVVPVALEVRRARRERATA"
     gene            complement(5720..>6064)
                     /locus_tag="VF954_01505"
     CDS             complement(5720..>6064)
                     /locus_tag="VF954_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529784.1"
                     /translation="VVLIAAVAFSKRSTGSMAGNGAGAGDDSGGPVMTGRRDVALAAA
                     IDRWIFDLGHEEHELRTRLVQVKAQLARGENAGPGTDEVGEPVAEVAASNGPPAAQAT
                     KATRTARKRASP"
BASE COUNT          861 a         2174 c         2240 g          789 t
ORIGIN      
        1 atcgacgggc ccacgcgctc gacgagcacg tagtcgggat gcacgcccga caggacccgc
       61 cggcagatct cgcacgagcc gcaaccacct gatccgcaca ggagggcggc cgcgaacgac
      121 cgggccgcgg tgcgcttccc cgcgcccggg ggcccgacga gcaggtaggc gtgcaccggc
      181 gcggcggcgg cggcgcgcag ctcggcaacg gctctgggtt ggcccaccac gtcgtcgaag
      241 agcgtcacgt caacgcccgg agccgcccga ccaccgatcg tgggcctggc cgacccgggc
      301 ggcgacctct tccaccggcc cggtgccgtc gaccacggcc cagcggtcgg ggtcggcgcc
      361 ggccagctgg cggaacccga gtcggacgcg atcgtgaaag cccccccgct cggactcgag
      421 ccggtcctcc gcctggcctg cgatgcggct ggccgccacc tcggccggga cgtcgaggag
      481 gatcacgagg tcgggctcga ggccgtcggc ggcccagccc gacaggcacc gcaggtcgga
      541 cagggccagt ccccgcccgt acccctggta ggcgtaggag gagccggcgt agcggtcgca
      601 gacgaccgtg cggcccgcgg ccaacgccgg gcggatgacc tcgacgacgt gctgggcccg
      661 ggccgcggcc atgagcaggg cctcggcccg gggatcgagc gcacccgatt ccgggtcgag
      721 cagcacggca cggatctgtt cgccgacctg agtcccaccg ggctcgcgcg tgagcacggc
      781 gccaagcgcc cgggccaggc gcgccgcctg cgtggacttg cccgacccct cgccaccctc
      841 gatgacgatg aagctcacgg gccttcgacc gccggggcgg ccaccaccgg gcccgccacc
      901 accgggcccg ccaccaccgc gcccgccacc accgggctgc ccaacgtgcg gcgggcgagc
      961 agcccggccc cgacgatgat gaggccggcc agccacagcg tgaggcggac tcccggcacc
     1021 gacaacgtcg ccgggccgag gtgcagctgc ccccggaccg tcgcggtgga cagcacgtcg
     1081 agcacccccg acaggaccgg cgcgaccgtc agggcgagca gcacgcagat gcggacggtg
     1141 gcgtagaggg tggcgaaggt acgaccccgt agctcgtcgg cgacgttggc ctgcaacagg
     1201 gagaagccca ggacgtaaac cgcgccggcg cacaggccga gggcgccgac acagaagcac
     1261 gccgggccga ggtggctcat ggccgcgccc agcaacaggc agggcccggc cgacaacagc
     1321 gcggccagaa agacgcgcac gtgatcgacc cggtgctgga cgagccccag gccgacgatg
     1381 ccgccggcga cgccaacgcc cagggacgcc agcagcactc cgaagccggc gggaccggcg
     1441 ccgaagacct gcttggccat gatcggtccg aggggcacga tcatggcgcc gccgatcatc
     1501 ccggtcccga gaccgatgat gaccgcccgc acgaccgggc tgtccacgac gaactgccat
     1561 ccctcgcgca gctccacgat cgtgcgcccg agatcgaagc cgcggtcgcc gctctcctgg
     1621 ggcttgggcc ggggcaggct caacgtggag atgaccgcgg ccgacaggaa gaacgtggcc
     1681 acgtcggccc acatggccag cgactcctgc ctgacaccta gggccggtgc cgcggcggcg
     1741 agggcggcga acagcgcggc ggcgaccggg aaggtgccgt aggccgcggc gagcgacagc
     1801 gagttggcgt tgggcaggaa cgacggcggc accaggttgg gcacgctcgc ctccttggcg
     1861 ggcgtccaca tgagcgtgag catctcgagc acgaaggagg ccgcgaccag ctcgccgacg
     1921 ttggtggcaa agggcaggag cgccagcacg gtgccgcgac ccacgtcgca ggcgaccatg
     1981 acccgccgcc ggtcccagcg gtccacgagc accccggcga aggacgccag gaacaggccc
     2041 ggcaccagcc gggcgatcag gaccaggctg atggccgcgc cgtcggagcc ggcgccgagg
     2101 cgataggcga gcgcggtgat ggcgaccagg ccgagccagt cgccgagcga ggacaccgcc
     2161 tgcgagcacc acagccgcgc gaacatgggc gagcccagcg ggtggccggg cgcgctcgtc
     2221 gggcccaccc cgtcgcgtgc cgcgcggagt cccccggtcg aggaatcggc cacgatcctc
     2281 aggctaacca atccattccc agaaccggtc gacggtgggc ggcgagcgct cagcccccgg
     2341 gccgcctggg ccgcgccttc ttggcgccgc ccgccttctt ggcgccggcg accttcttgg
     2401 ccggccgggc ccgcttcgcc ggagggccca tctcgcggcg ccgggcgagc agctcggcgg
     2461 cccgctcaga tgtgagctcc tcgggcgagt ctccccgggg cagcgatgcg ttgctctccc
     2521 cgtcggttac gtacgggccg aagcggcctg atctgaggac catctgccgg cccgtcgccg
     2581 ggtccgggcc gagctcccgc atcggcgcgg catcctggcg gccccgaccg cgcttgggtt
     2641 gggcgaacag ctccagggcc tgctcgacgg tgacggtgaa caggtcctcc tctgcggcca
     2701 tggtgcgggt gtctgatccc ttggtgaggt acggcccgta gcggccgttc tgggcgacga
     2761 tctcctgacc gtccggggcc gcgccgacga cccggggcag gctcaggagc ctgaggccct
     2821 cgtcgagggt gaccgtgccc gggtccatgg ccttgaagag cgacgccgtc gccgggcggg
     2881 tcttgctccc cggctcggcg tcaccgagct gcacgtacgg gccgtagcga ccggccttga
     2941 ccacgaccgg cagaccggag gcggggtcgg tgccgagggc gcggtcgccc gcgggggcga
     3001 caaggagctg ctccacccgc tcggcggtca gctcgtcggg agccacgtcg tcagggatgg
     3061 acgcccgctg gtcgccccgc tggacgtagg ggccgtagcg accgacgcgc acgaccaggt
     3121 cgcgtccctc ggcgtccacc ccgatcggga tggagttgat ctcccgggcg tcgatgtcgt
     3181 ccaggtgctc ggagaccagc ctcttcaagc cgggaaggcc gttgccgaag tagaacgtgc
     3241 gcagccaggg gacgacctcg gcctggcccc cggcgatctc gtcgaggacc ccctccatgg
     3301 acgcggtgaa gccgtagtcc accagggcgc cgaagtgcgc ctcgagcagc gccgtgaccg
     3361 cgaacgcgat gaaggaaggc acgagggccg tgcccttctt ccacacgtag ccccggtcct
     3421 ggatggtgcc gaggatggtg gcgtacgtcg agggccgacc cacgccgagc tcctcgaggg
     3481 ccttgaccag cgatgcctcg gtgaagcggg ccggcggctg ggtcgtgtgg gtcttggcct
     3541 ccacggcgac cgtcgacacg gcgtcgcctt gccgcatggc gggcagccgc acttcctggt
     3601 cctccagctc ggcgtcgggg tcgtcggccc cctcgacgta ggcgcgcatg aagccgggga
     3661 aggtgatgac cttgccgctg gcggccagct cgacgtcctc gccgccgtcc ggggccacgc
     3721 cccccaggcg gacctgggcg gactggccgc gggcgtcggc catctgggag gcgaccgtcc
     3781 gcttccagat gagctcgtag agccgggcct cgtcaccgtt gagcccggct tcgtcgggca
     3841 tgcgaaagac ctcgcccgcg ggccggatgg cctcgtgggc ctcctgggcg ttcttgacct
     3901 tcttctcgta acgccggggc gagtcgggca cgtagtcggg gccgtacagg gcccgggcct
     3961 gcgaccgggc ggcgtccatc gccgtgctgg acagggtggt ggagtcggtc cgcatgtagg
     4021 tgatgaagcc gttctcgtac agccgctggg ctatcgacat cgtccgttgc gaggggaacc
     4081 gcagcttgcg acccgcctcc tgctggaggg tcgaggtcat gaacggcggg tggggggagc
     4141 ggcggaaggg ccgctcctcg acgctgcgca ccgacaacgt cgagccggcc aggcgatcgg
     4201 cgagcgaccg ggccgcgctt tcgtcgagac ggaccaccgc ggcgttcgcc ctgagggcgc
     4261 cgtcctcgcc gaagtcccgc cccgtcgcca ccctcgtacc gtcgaccgcc accaggccgg
     4321 cggtgaacga cggcggcgag gtcggtgcgt cgaaggtgcc ctcgatgtcc caccagcccg
     4381 ccgaccggaa gcgcatccgg gcccgctccc ggtcgaccag gatgcgcgtg gcgacgctct
     4441 gcacccggcc ggccgacagc cgcggcatga ccttcttcca gagaacgggc gagacctcgt
     4501 agccgtagag ccggtcgagg atgcgccggg cctcctgggc gtcgaccagg tggcggtcca
     4561 gctcgcgcca gtcgtcgacg gcctgctcga tggcggggcg ggtgatctcg tggaagacca
     4621 tccgcttgac gggcactcgc gggttgagca cctcgaggag gtgccaggcg atggactcgc
     4681 cctcgcggtc ctcgtcggtg gccagataga gctcgctggc gtccttgagg agggccttca
     4741 gcttggcgac ctgggacttc ttctccgggg cgacgacgta gaggggcttg aagtcgttgg
     4801 cggtgtcgac gccgagccgg gcccaggcct ctcttttgta ggccgcgggc acgtcggccg
     4861 cgtttcgggg gaggtcgcgg atgtggccga tggaggactc caccacgaag tcgcggccga
     4921 ggaacccggc gatggtcttg gccttggagg gcgactccac gatgacgagt ggcttgggca
     4981 tggctcctaa gaggttgggc ggcgagaacg gcgatgtcaa aggggaccct accgaaaccc
     5041 tccctgaccg gtgggcggcg agacgtcggc gactacggtg accgggtgca cgccctggac
     5101 ccgacccatc tgctcgacac gtacggcatc tgggggatct acgccgtaat cttcgccgag
     5161 accgggctgc tcatcgggtt cttcctgccg ggcgactcgc tgctcgtgac ggccgggctg
     5221 ctcgcctcga ccggcaagcc gggcgacgtg cacctcaacc tggccgccct gctggtgggc
     5281 tgcgcggtgg cagcggtcgc aggggcccag gccgggttcg tcatcgggcg gcgggtcggg
     5341 ccggcgctgt tccggcgacc ggactcgcgc atcttcaagc agcgctacgt cgagcggacc
     5401 cacgagaccc tcgcccggta cggcgcggtc aaggccatcg tgctggcccg cttcatcccg
     5461 gtggtgcgca cgttcctgaa cccggtggcc ggcgtcgggg gcgtcgacac ggggctgttc
     5521 accgcggcca acctgggcgg gggggtggcc tggacgacca gcatggtgct gctcggctac
     5581 accgtgggca gcaccgtgaa ggggatcgac cgttacctcg tgccggccat catcgtgatc
     5641 tcggtcgtcc cggtggcgct ggaggtgcgg cgggcacggc gcgagcgggc cactgcctag
     5701 cgatcagccg gttggctcgt catggggagg cccgcttgcg ggcggtccgg gtggccttcg
     5761 tcgcctgcgc cgccggtggg ccgttggacg ccgccacctc cgctaccggc tcaccgacct
     5821 cgtcggtgcc cgggccggca ttttccccac gagccagctg cgccttgacc tggacgagac
     5881 gggtcctcag ctcgtgctcc tcgtggccga ggtcgaagat ccaccggtcg atggcggccg
     5941 ccagcgccac gtcgcggcgc ccagtcatga ccgggccacc gctgtcgtcg ccggccccgg
     6001 ccccgttgcc cgccatggag cccgtgctgc gcttcgagaa ggccacggcc gcgatcagca
     6061 ccac
//
LOCUS       DASWGG010000087         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2987013, whole genome shotgun sequence.
ACCESSION   DASWGG010000087 DASWGG010000000
VERSION     DASWGG010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2987013"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VF954_01510"
     CDS             complement(<1..890)
                     /locus_tag="VF954_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9529785.1"
                     /translation="MTPSSSHHQDLRHLAAGYAVLASLAVSASLVAIAARPPASLVVL
                     VLACGVTVGGIPGWRRVLALQSHRGLDEALEVDLERKRRIQAVLNAGDTLSIVFQPVI
                     DLTSNLTVGWEALTRFHGDDLSPRVWFEEAGAVGLAVELELLAVSRALDSAPRPEYLA
                     INVGPATLVDERFIALMRRRRQGPLVVELTEHAVIDDYAVVIAALDQLRQLGIRVAVD
                     DAGSGFSSLRHVISIAPDIIKLDQALIASIDRDQRRRTLATSLATFAAEIGSELVAEG
                     IEREEELRVCEEIGIRFGQG"
     gene            complement(1084..>1704)
                     /locus_tag="VF954_01515"
     CDS             complement(1084..>1704)
                     /locus_tag="VF954_01515"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9529786.1"
                     /translation="GCGVRGLTTSFRVDRCFAFVDLCGFTDYVDAEGDDAAVRELGKL
                     RSAVREVAPLCGVRVDKWLGDGVMLVGVESEPVVRGVLAIGQRFHDDHTHLALRAGMA
                     AGPVILLEGDDYIGRSVNLAARLCDVADAYVVLAAPSETLVLPEDVRTLPAGDMVVKG
                     FARPVEVVAIEAVRPSDRFDGRRRGTIGLRLPSLDQLLRPRARPSA"
BASE COUNT          263 a          602 c          621 g          218 t
ORIGIN      
        1 aagccctggc cgaagcggat cccgatctcc tcgcagaccc gcagctcctc ctcccgctcg
       61 atgccctcgg ccaccagctc ggagccgatc tcggccgcga aggtggccag gctggtggcg
      121 agcgtgcggc gccgctggtc ccggtcgatc gacgcgatca gggcctggtc gagcttgatg
      181 atgtcgggag cgatggagat gacatggcgc agcgacgaga agccgctgcc ggcgtcgtcg
      241 actgccaccc ggatgcccag ctggcgcagc tggtcgaggg cggctatgac caccgcgtag
      301 tcgtcgatga cggcgtgctc ggtgagctcg accacgagcg gaccctgccg ccgccgccgc
      361 atgagcgcga tgaagcgctc gtcgaccagg gtcgccgggc cgacgtttat ggccaggtac
      421 tcgggccggg gggcggagtc gagggcccgg gacaccgcca gcagctccag ctccacggcg
      481 agccccaccg cgccggcctc ctcgaaccac acacgcgggg acaggtcgtc tccgtggaag
      541 cgggtgaggg cctcccagcc gacggtcagg ttgctggtga gatcgatgac gggctggaag
      601 acgatggaga gcgtgtcccc ggcgttgagc accgcctgga tccggcgctt gcgctccagg
      661 tcgacctcga gcgcttcgtc caggccccgg tgcgactgga gggcgagcac ccgccgccag
      721 ccggggatgc cgccgacggt gacgccgcac gccaggacga gcactaccag gctcgccggc
      781 gggcgcgccg cgatcgccac caggctggcg gagaccgcga gcgaagccag gaccgcgtag
      841 cccgccgcca ggtgccggag gtcctggtgg tgcgagctgc ttggtgtcat ggagcggagg
      901 cctcccgggg tggcccgcct ccgcatatgg ctagacgggg aggcgatgtg gttcttgcca
      961 gcggtatgtg actagtcggc atggactagt ccgaccttga gcaagacgtc tcgtggggcc
     1021 acgctaactc cctgagaccg tcaaaatgga catccctccg ggtccgcgcc catgcggccc
     1081 tggtcaggcc gagggccggg cccgggggcg gagcagctgg tcgagggacg gcagccgcag
     1141 gccgatcgtc ccccggcggc ggccgtcgaa gcgatcggac ggccgcacgg cctctatggc
     1201 caccacctcc acgggacggg cgaacccctt gaccaccatg tcgccggcgg gcagggtgcg
     1261 gacgtcctcg ggcagcacca gcgtctcgga cggcgcggcc agcaccacgt aggcgtcggc
     1321 cacgtcgcag agccgggcgg ccaggttcac ggaccggccg atgtagtcgt cgccctcgag
     1381 caggatcacc gggccggcgg ccatgcccgc ccgcagcgcc aggtgggtgt ggtcgtcgtg
     1441 gaagcgctgc ccgatggcga gaacgccccg gacgaccggc tcggactcga cgccgacgag
     1501 catcaccccg tcgccgagcc acttgtcgac tctcacgccg cacaggggcg ccacctcccg
     1561 cacagccgat cggagcttgc ccagctcgcg cacggcggcg tcgtcaccct cggcgtcgac
     1621 gtagtcggtg aagccgcaga gatcgacgaa ggcgaagcac cgatcgacgc ggaagctcgt
     1681 ggtcaggccg cgcacgccgc accc
//
LOCUS       DASWGG010000088        11491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2669329, whole genome shotgun sequence.
ACCESSION   DASWGG010000088 DASWGG010000000
VERSION     DASWGG010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11491
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2669329"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VF954_01520"
     CDS             <1..380
                     /locus_tag="VF954_01520"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378372.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX9529787.1"
                     /translation="ALAAGLAAEGFRLVSGGTDNHLLLVDLRTFDPDLTGKAAQEALD
                     RAGITTNKNTVPNDPRSPFVTSGLRLGTAATTTTGMGPADMGEIARLIGHTLRHRGSD
                     GELASVKDEVANLCSKFPPYPAE"
     gene            397..1554
                     /locus_tag="VF954_01525"
     CDS             397..1554
                     /locus_tag="VF954_01525"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX9529788.1"
                     /translation="MLAFAVVFATAAVGTWLLTFAVRRAAVRFGAVVLPDERRVHEKP
                     TPTAGGAAMFLAFLAAMGVASQLPAFAPVFHGSSEPLGVVLAGGVVFLVGLIDDVREV
                     SPPAKLAGTVLAGSVLYFLGVNMFYFRIPFADFVVLSASLTPLITVLWVAGMTTVVNY
                     IDGLDGLAAGIVAIGAAAFFVYGNRLTNAGLLSPDNIGPLLAVIACGVCVGFLPHNFH
                     PARIFMGDAGALFLGLLMASSTMVVGGRTADQFSGQTFFFFAPLVIPFVILGVPMLDT
                     LFSIVRRAARRTSVARPDKDHLHHRLMRLGHGQRRSVLILWAWTAILSGMVLYPTFTN
                     HGNAVIPFGIAGLAVLLYTMFHPGVRQRGTADGLAEVIALDGTESARRRSR"
     gene            1730..2017
                     /locus_tag="VF954_01530"
     CDS             1730..2017
                     /locus_tag="VF954_01530"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX9529789.1"
                     /translation="MQTNRDSRDKRDLYHGFSESFGHAVELACTPVLFGGAGWWLDHR
                     LGIFPVLTIALVVLCLVGMFARMYYAYEARMQAHDTEAVWGRPGATGRGPV"
     gene            2014..2493
                     /locus_tag="VF954_01535"
     CDS             2014..2493
                     /locus_tag="VF954_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX9529790.1"
                     /translation="MSPLLTAGRSARTAERPPLASVRDEGPAYERDVALDMVRRGLFA
                     APALVAAAWLVWGPAGAWSSAFAIGLVLVNFLLAAFLLGWAARISLAALMGTALAGYL
                     LRLGLITAAVLAVRRQPWVKVMPLGLSIIVTHLGLLLWETRFVSISLAAPGLREKGS"
     gene            2497..3270
                     /gene="atpB"
                     /locus_tag="VF954_01540"
     CDS             2497..3270
                     /gene="atpB"
                     /locus_tag="VF954_01540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX9529791.1"
                     /translation="MHHLLAFEFPPLGELIVWKDLFGTHGWYGLNKVGLECLLATVLT
                     LVIFLMAGRRGALVPTGVQNVAEMVVDFIRDGIILQTMGPEGLGWGMFFTTLFMFIFF
                     CNIFEIVPGIQMPATARMGIPLVLALIAWVCYHVTGIKHQGVLAYFKSATIPPGVPLA
                     MLWLVFFIELLQILVLRPFSLAVRLFANMLAGHLLLVTFAVLTKTVLHPNPLALVTPL
                     TIAMEIAIAGFEILVSLLQAFIFTILTAVYIGGAMHPEH"
     gene            3328..3594
                     /gene="atpE"
                     /locus_tag="VF954_01545"
     CDS             3328..3594
                     /gene="atpE"
                     /locus_tag="VF954_01545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX9529792.1"
                     /translation="MAPVLAQVASNGTTTLKGLGAVGAGLAYGLAAIGPGIGIGIVVG
                     NAITAMARQPEAAGMARTTMFLGIAFTEALALIGFVVAILVLSK"
     gene            3604..4143
                     /gene="atpF"
                     /locus_tag="VF954_01550"
     CDS             3604..4143
                     /gene="atpF"
                     /locus_tag="VF954_01550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX9529793.1"
                     /translation="MLALIAAATDSTQKAPNPIVPSGNEIFWGSVAFVVLVVLMWKFA
                     YPAVTKAMEDRTNRIRKDLDDAERVRGEAQTILEEYQRQLSDARGEANRIIEEARQTA
                     DQLRRDLMARAEQEVNELKSRSAQDIESARERAVSDLRNQVAGLAIELAEVVVKRNLD
                     HDTNIHLVEEYISQVGSGS"
     gene            4143..4673
                     /gene="atpH"
                     /locus_tag="VF954_01555"
     CDS             4143..4673
                     /gene="atpH"
                     /locus_tag="VF954_01555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX9529794.1"
                     /translation="MAAGDRVDAYASALFEVAKAEGSLETVEDELFRVARTLEGSDEL
                     RATLTDERIPVETRQGIVEDLLGDRAAPVTTALVSFVVGSGRGRDLPAIIDMLVKRAA
                     EERQEAVADVRTSIPLDGDQLRRLAEALSKATGKRLSLKVTVDPSILGGVVAQIGDTI
                     IDGSVRHSLEQLRESL"
     gene            4730..6355
                     /gene="atpA"
                     /locus_tag="VF954_01560"
     CDS             4730..6355
                     /gene="atpA"
                     /locus_tag="VF954_01560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX9529795.1"
                     /translation="MAEPMFTFDSQDISATIRRRLESFRPRVAQEQIGRITEVGDGIA
                     RVAGLPLTSVNELLEFEGGVLGIALNLDEETIGAVILGETTHIEEGQPVRGTGRILSI
                     PVGDAMLGRVVNPLGEPIDGKGPVASEHVRRIEIQAPGVVDRQPVKEPLQTGIKVIDA
                     MTPIGRGQRELIIGDRKTGKTAVAVDTIINQRGLGVKCIYVGIGQKSSTVAELVATLE
                     AAGAMDYTVVVNAPASDPSPFKYLAPYAGCAMGQHWMENGEAALVVYDDLSKQAEAYR
                     QMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDALGGGSLTALPVIETKAGDISAY
                     IPTNVISITDGQIFLDLDLFNADVRPAMNVGTSVSRVGGSAQIKAMKAVSGGLKLDLA
                     NFRELEAFASFGSELDKASQALIDRGRRLVELLKQTNGKPLSIDEQVVSIFAGTSGFL
                     DDLDPADVRRFEGELITDVRSRKPQVMETIRTGGALPEDDLREAIGEFKARFQAGAPA
                     PTGVGGASGAAADGAPAAAAPSTPAAAPVGDRA"
     gene            6363..7301
                     /locus_tag="VF954_01565"
     CDS             6363..7301
                     /locus_tag="VF954_01565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004145.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX9529796.1"
                     /translation="MAGGQERVLRRRIKSVQSTRKITRAMELIAASRIVKAQQRVAAA
                     RPYAEHITEVIRSVASAGANLNAPLLVERTEIRRVAFVVIAADRGLAGGYNGNVLRAA
                     ERALKQQQAQGRDYALYLTGRKAINYFRFRRYRIDASWSGFSEFPQYEDARRVAEVLL
                     TDFDEGRIDQVELVYTQFLSLATQRVLVRRFMPLDTGTLADAPRDEVHSDYEYEPSPD
                     GILQALLPRYVEARLFAAMLDAAASEHAARQRAMKSATDNADALTTVLQRAMNRARQE
                     SITTEIMEIVGGAEALGGARKRKAHQHEEAEYEEHV"
     gene            7301..8752
                     /gene="atpD"
                     /locus_tag="VF954_01570"
     CDS             7301..8752
                     /gene="atpD"
                     /locus_tag="VF954_01570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX9529797.1"
                     /translation="MTPAAKAAIAEPELKEGRIVAIVGPVVDVEFPPDGLPELNGALE
                     FDVEVEGEAQTIVAEVAQHIGESRVRAICMRPTDGLKRGAAVRNLGHGIRVPVGNATL
                     GHVWNVIGRPLDTRGAEVEGITEYWDIHRPPPAFADLDPETRMFETGLKVVDLLEPYR
                     LGGKIGLFGGAGVGKTVMIQEMIIRVARQHGGVSVFAGVGERTREGNDLWLEMQESGV
                     IANTALVYGQMDEPPGVRLRVALSALTMAEYFRDVQNQDVLLFVDNIFRFTQAGSEVS
                     TLLGRMPSAVGYQPTLADEMGELQERITSVKGRSITSLQAVYVPADDYTDPAPFTAFT
                     HFDATTNLDRAVFAKGIFPAVDPLASTSNILQPSIVGEDHYNVARRIIQVLQRYKELQ
                     DIIAILGIDELSEEDRLLVGRARKAEKFLGQPMYAGTPFTGIEGTNVPVAETVEAFGN
                     LLDGEYDDVPEQAFFNVGGTEDLMAKAKRLREA"
     gene            8761..9144
                     /gene="atpC"
                     /locus_tag="VF954_01575"
     CDS             8761..9144
                     /gene="atpC"
                     /locus_tag="VF954_01575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX9529798.1"
                     /translation="MQVALVSPERILFEGEADMVVGRTSDGEIAFLTGHAPFLGALGI
                     GAVKIVATDGRQERAAVHGGFVEVRGNRVTILSDVAELASQIDLERARAAAADAERRL
                     MTADDVECEARLRRAHVRIAVATAT"
     gene            9200..10168
                     /locus_tag="VF954_01580"
     CDS             9200..10168
                     /locus_tag="VF954_01580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9529799.1"
                     /translation="MARVRLGVALLLPRDQAAEVQGLRRALGDPALDRIPPHLTLVPP
                     VNVRAEDMPGALLRLREAAAATAPFSVELGPPSTFLPDSPVLFLAVDDAAGAVARLRE
                     RVFHPPLARPLTWPFVPHMTLLDGGGPGRLEAARWAMAGFRIAVEWAELHLLQEEPGR
                     IWRSLASAVLGGSAVIGRGGLPLEISRSTQPDPAARRFAEQAWAELDLAETGGLAPRV
                     DMAFTARRSGQVVGVATGRAEGGVGHLSELIVASAVRGEGVGHHLLAAFESWAAGEGV
                     TRLALRTPAGGPAEGFYARRGWREEARLTDWYGGRSFVQLRRDLAP"
     gene            complement(10342..11337)
                     /gene="glpX"
                     /locus_tag="VF954_01585"
     CDS             complement(10342..11337)
                     /gene="glpX"
                     /locus_tag="VF954_01585"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX9529800.1"
                     /translation="MNAARQAPDRNLALELVRVTEVAALAASRWMGRGDKEGADGAAV
                     DAMRVVLETIAMDGVVVIGEGEKDDAPMLYNGERIGNGSPPETDVAVDPIDGTTLTAK
                     GRGNALSVIAVSERGTMFDPGPCVYMEKLAVGPEAAGLVDITRSVAENLETVAKAKGS
                     TVRDVTAVILDRDRHDTLIAEVRAAGARIRLIPDGDVAGAISTAWPDSGADILFGIGG
                     TPEGVIAAAALKCMGGELQGRLWPRTADERRTAVDAGYDLSKVLTTDDLVAGDNCFFV
                     ATGITDGELLRGVHYDSKGASTQSLVMRSKSGTVRRIDARHRLPKLQEFSIIEFG"
BASE COUNT         1601 a         4236 c         3909 g         1745 t
ORIGIN      
        1 aggcccttgc ggccgggctg gcggcggagg ggttccggct cgtctcgggg gggacggaca
       61 accacctgct tctcgtcgac ctgcgcacct tcgatccgga cctgaccggg aaggcggccc
      121 aggaggcgct cgaccgggcg ggcatcacga ccaacaaaaa cacggtgccc aacgatccca
      181 ggtccccgtt cgtgaccagc ggcttgcggc tcggcacggc cgccaccacc accacgggca
      241 tgggaccggc cgacatgggc gagatcgccc gcctcatcgg ccacacgctc cggcaccggg
      301 gcagcgacgg cgagctggcc tcggtgaagg acgaggtcgc caacctgtgc tcgaagttcc
      361 cgccgtaccc ggctgagtaa gtagggtcgc tgctccatgc tggccttcgc ggtcgtcttc
      421 gccaccgccg cggtcggcac ctggctgctc accttcgccg tgcgtcgggc cgccgtgcgg
      481 ttcggggcgg tggtcctgcc cgatgagcgc cgggtgcacg agaagccgac gcccacggcc
      541 ggcggggcgg ccatgttcct cgccttcctg gcggccatgg gcgtcgcctc ccagctgccg
      601 gccttcgcgc ccgtcttcca cgggtcctcg gagcccctcg gcgtggtcct ggcgggcgga
      661 gtggtcttcc tggtcggcct catcgacgac gtccgcgagg tgtcgccgcc ggccaagctg
      721 gcgggcacag tgctggccgg gtccgtcctg tacttcctgg gcgtcaacat gttctacttc
      781 cggatcccgt tcgccgactt cgtcgtgctg tcggccagcc tcaccccgct gatcaccgtg
      841 ctgtgggtcg ccggcatgac gacggtggtc aactacatcg acgggctcga cggcctggcg
      901 gcagggatcg tggccatcgg cgcggccgcc ttcttcgtgt acggcaaccg gctgaccaac
      961 gccgggctgc tctcgccgga caacatcggc ccgctgttgg cggtcatcgc gtgcggcgtc
     1021 tgcgtcgggt tcctgcccca caacttccac ccggcccgga tcttcatggg cgacgccggg
     1081 gcgctgttcc tgggcctgct catggcctcg tccaccatgg tcgtcggcgg ccggacggcc
     1141 gaccagttca gcgggcagac gttcttcttc ttcgctcccc tggtcatccc gttcgtcatc
     1201 ctcggcgtgc cgatgctgga cacgctgttc tcgatcgtac gaagagcggc ccgccgtacg
     1261 agcgtggccc gtccggacaa ggaccacctc caccaccggc tcatgcggct cggccacggg
     1321 cagcgccgct cggtgctgat cctgtgggcg tggacggcga tcctgtcggg catggtcctg
     1381 taccccacgt tcaccaacca cggcaacgcc gtcataccct tcggcatcgc cggcctggcc
     1441 gtgctcctgt acaccatgtt ccaccccggc gtgcgccagc gcggcacggc cgacggcctg
     1501 gccgaggtca tcgccctcga cggcacggag tcggcccgcc gccgctcccg gtgagccgca
     1561 gccggcgctg acccggggcc gcggggttgc gtcctgtggg ggacctcgca ctaagttttg
     1621 cgcccggtgt gggtttgagt ttgcgagccc gaaacctgtg tcatactttg tgcaaatctt
     1681 cacaaggccg gcgtcgacct gtgccggtct ccctcgtcga ggatcttggg tgcagaccaa
     1741 ccgcgactcc cgcgacaagc gcgacctcta ccacggcttc agcgagtcgt tcgggcacgc
     1801 cgtcgagctg gcgtgcacgc cggtcctgtt cggcggggcc ggctggtggc tcgaccaccg
     1861 gctcgggatc ttcccggtcc tgaccatcgc gctggtcgtg ctctgcctgg tgggcatgtt
     1921 cgcccgcatg tactacgcct acgaggccag gatgcaggcc cacgacaccg aggcggtttg
     1981 gggccggcca ggcgccaccg gcaggggccc ggtgtgagcc cgctcctcac ggccgggagg
     2041 tccgctcgca ccgccgagcg gccgccgctc gcctccgtcc gcgacgaggg ccccgcctac
     2101 gagcgcgacg tcgccctgga catggtcagg cggggcctgt tcgccgcgcc ggccctggtc
     2161 gccgcggcct ggctcgtgtg gggcccggcc ggggcgtggt cgtcggcgtt cgccatcggc
     2221 ctcgtgctgg tgaacttcct gctggccgcc ttcctgctcg gatgggcggc gcgcatctcg
     2281 ctcgccgccc tgatgggcac ggccctggcc ggctacctgc tccgtctggg gctcatcacc
     2341 gccgcggtgc tcgcggtcag gcgtcaaccg tgggtgaagg tcatgcccct cggcctctcc
     2401 atcatcgtca cccacctcgg gctcctcctg tgggagaccc gcttcgtgtc catctccctc
     2461 gccgcacccg gccttcgcga gaaagggtcg tgacccttgc accacctgct ggccttcgaa
     2521 ttcccaccgc tcggtgagct gatcgtctgg aaggacctct tcgggaccca cggttggtac
     2581 gggctcaaca aggtcggcct cgagtgcctc ctggccactg tccttaccct cgtcatcttc
     2641 ctgatggccg gccggagggg tgccctcgtg ccgaccggtg tccagaacgt ggccgagatg
     2701 gtggtcgact tcatccggga cgggatcatc ctccagacga tgggcccgga ggggctgggc
     2761 tgggggatgt tcttcacgac gctgttcatg ttcatcttct tctgcaacat cttcgagatc
     2821 gtgcccggca tccagatgcc ggccaccgcc cgcatgggca tccccctcgt cctcgccctg
     2881 atcgcgtggg tctgctacca cgtcaccggg ataaagcacc agggcgtgct cgcctacttc
     2941 aagtcggcga ccatccctcc gggcgtgccg ctggccatgc tctggctcgt cttcttcatc
     3001 gagctgctcc agatcctcgt cctgcggccg ttctcgctcg ccgtccggct cttcgccaac
     3061 atgctcgccg gccacctgct gttggtgacc ttcgcggtcc tcaccaagac cgttctgcac
     3121 cccaacccgc tggctctggt cacccctctg accatcgcca tggagatcgc catcgccggc
     3181 ttcgagatcc tggtctctct cctccaggcg ttcatcttca ccatcctcac cgccgtgtac
     3241 atcggcggcg ccatgcaccc cgagcactga tccaccctcc ggaagcccag cttccggagg
     3301 tcccaaccca ctaggaggag ctctcccttg gcacctgtcc tcgcccaagt cgccagcaat
     3361 ggcaccacca cgctgaaggg cctcggcgcc gtcggcgccg gcctggccta cgggctggcg
     3421 gccatcggcc ccggcatcgg catcggcata gtggtgggca acgccatcac cgccatggcc
     3481 cgacagccgg aggccgccgg catggcgcgg accaccatgt tcctcggcat cgcgttcacc
     3541 gaagcccttg ccctcatcgg cttcgtcgtg gccatcctcg tcctctcgaa gtaggagaca
     3601 tctgtgctcg cactgatcgc ggcagccacg gacagcacgc agaaggcgcc caaccccatc
     3661 gtgccgtcgg gcaacgagat cttctggggc tcggtcgcct tcgtggtgct ggtcgtcctc
     3721 atgtggaagt tcgcctaccc ggcggtgacc aaggccatgg aggaccgcac caaccgcatc
     3781 cgcaaggacc tcgacgacgc cgagcgggtt cggggcgagg cccagaccat cctcgaggag
     3841 taccagcgcc agctcagcga cgcccggggc gaggccaacc ggatcatcga ggaggcccgc
     3901 cagaccgccg accagctgcg ccgggacctg atggcccggg ccgagcagga ggtcaacgag
     3961 ctcaagtcgc gaagcgccca ggacatcgag tccgcccggg agcgcgccgt gtccgacctg
     4021 cgcaaccagg tggccggcct cgccatcgag ctcgccgagg tggtggtcaa gcgcaatctc
     4081 gaccacgaca ccaacatcca cctcgtcgag gagtacatca gccaggtcgg gtccggcagc
     4141 tgatggccgc cggggaccgt gtcgacgcgt acgcgtcggc cctcttcgag gtggccaagg
     4201 ccgagggctc gctcgagacc gtggaggacg agctgttccg cgtcgcccgg acgctggagg
     4261 gcagcgacga gctgcgcgcc accctcaccg acgagcgcat cccggtcgag acgaggcagg
     4321 ggatagtcga ggacctgctc ggcgaccgcg ccgctcccgt cacgaccgcc ctggtgtcct
     4381 tcgtcgtcgg ctccggccga gggcgcgacc tgccggccat catcgacatg ctcgtcaagc
     4441 gggcggccga ggagcgccag gaggcggtcg cggacgtccg cacctcgatc cccctcgacg
     4501 gggaccagct ccgccggctg gccgaggcgc tctccaaagc gaccggcaag cggctgtcgc
     4561 tgaaggtgac cgtcgatccc tccatactcg gcggcgtggt ggcccagatc ggcgacacga
     4621 tcatcgacgg cagcgtccgg cacagcctcg aacagctccg ggagtcgctc tgacgatgcc
     4681 gagatcacgc ccacgtccgg caaccgcgaa aggcacggga agcccctcca tggccgagcc
     4741 catgttcacc ttcgactcgc aggacatatc cgccaccatc aggcgccggc tcgagagctt
     4801 ccgcccacgg gtcgcccagg agcagatcgg ccgcatcacc gaggtgggcg acggcatcgc
     4861 ccgggtcgcc ggcctgccgc tgacgtcggt caacgagctg ctggagttcg agggcggggt
     4921 gctcggcatc gccctgaacc tggacgagga gacgatcggc gcggtcatcc tgggcgagac
     4981 cacccacatc gaggagggcc agccggtgcg ggggacgggc cgcatcctgt ccatcccggt
     5041 cggcgacgcc atgctcggcc gggtggtgaa cccgctgggc gagcccatcg acggcaaagg
     5101 gcccgtcgcc tcggagcacg tccggcgcat cgagatccag gcccccggcg tcgtggaccg
     5161 ccagccggtg aaggagcccc tccagaccgg catcaaggtg atcgacgcca tgacgcccat
     5221 cggccggggc cagcgggagc tcatcatcgg cgaccgcaag accggcaaga cggcggtggc
     5281 ggtggacacc atcatcaacc agcgcggcct cggggtgaag tgcatctacg tcgggatcgg
     5341 ccagaagtcc tcgaccgtgg ccgagctggt cgccaccctc gaggccgccg gtgccatgga
     5401 ctacaccgtc gtggtcaacg cgcccgcctc cgacccctcg ccgttcaagt acctggcccc
     5461 gtacgcaggc tgcgccatgg gccagcactg gatggagaac ggcgaggccg ccctcgtggt
     5521 ctacgacgac ctctccaagc aggccgaggc ctaccgccag atgtccctgc tgctgcgccg
     5581 gccgccgggg cgcgaggcct acccgggcga cgtgttctac cttcacagcc gcctgctgga
     5641 gcgggccgcc aagctctccg acgccctcgg cggcgggtcg ctcacggcgc tcccggtcat
     5701 cgagaccaag gcgggtgaca tctcggccta catcccgacc aacgtgatct cgatcaccga
     5761 cgggcagatc ttcctcgacc tggacctctt caacgccgac gttcgcccgg ccatgaacgt
     5821 tggcaccagc gtgtcccggg tcggtggctc cgcccagatc aaggccatga aggcggtctc
     5881 cggcggccta aagctcgacc tggccaactt ccgcgagctg gaggccttcg cgtcgttcgg
     5941 ctcggagctg gacaaggcgt cccaggccct gatcgaccgg ggccggcggt tggtcgagct
     6001 gctcaagcag accaacggga agcccctctc gatcgacgag caggtcgtgt ccatcttcgc
     6061 cgggacgagc ggcttcctcg acgacctcga cccggccgac gtgcgccgct tcgagggcga
     6121 gctgatcacc gacgtccgca gccgcaagcc ccaggtcatg gagacgatcc gcaccggcgg
     6181 cgccctgccc gaagacgacc tgcgcgaggc gataggcgag ttcaaggccc ggttccaggc
     6241 cggcgccccc gcgcccaccg gagtcggcgg cgccagcggc gcggccgccg atggcgcccc
     6301 tgccgccgcg gcgccgtcca ccccggcggc ggccccggtg ggggacaggg cgtagccggg
     6361 ctatggcggg cggccaggag cgcgtcctgc ggcgccggat caagagcgtc cagtcgaccc
     6421 gcaagatcac gcgggccatg gagctcatcg cggccagccg catcgtcaag gcccagcaac
     6481 gcgtggccgc ggcgcgtccc tacgccgagc acatcaccga ggtcatccgc agcgtggcgt
     6541 ccgccggtgc caacctcaac gccccgctgc tggtcgagag gaccgagatc cggcgggtgg
     6601 ccttcgtggt catcgccgcc gaccgcggcc tggcgggcgg ctacaacggc aacgtgctgc
     6661 gcgccgccga gcgggccctg aagcagcagc aggcgcaggg ccgcgactac gccctgtacc
     6721 tcaccggccg caaggccatc aactacttcc gcttccgccg ctatcgcatc gacgcgtcct
     6781 ggagcgggtt ctccgagttc ccccagtacg aggacgcccg gcgggtggcc gaggtcctgc
     6841 tcaccgactt cgacgaaggc aggatcgacc aggtcgagct ggtttacacg cagttcctct
     6901 ccctggccac ccagcgggtg ctggtccgcc ggttcatgcc cctggacacc ggcaccctcg
     6961 ccgacgcccc ccgggacgag gtccactccg actacgagta cgagccctcg cccgacggca
     7021 tcctccaggc cctcctgccc cgctacgtcg aggcccggct cttcgcggcc atgctggacg
     7081 ccgccgcgtc cgagcacgcc gcccgccagc gggccatgaa gtccgccacc gacaacgccg
     7141 atgctctcac gaccgtgctc cagcgggcca tgaaccgggc ccgccaggag tccatcacca
     7201 ccgagatcat ggagatcgtg ggcggggcgg aggccctggg cggcgcccgc aagcgcaagg
     7261 cgcaccaaca cgaagaagcc gagtacgagg agcacgtctg atgacccccg ccgccaaagc
     7321 cgcgattgcc gagcccgagc tcaaggaagg gcgcatcgtc gccatcgtcg gccccgtggt
     7381 cgacgtggag ttcccgccgg acggcctgcc ggagctcaac ggcgcgctcg agttcgacgt
     7441 cgaggtcgaa ggcgaggcgc agaccatcgt ggccgaggtc gcccagcaca tcggcgagag
     7501 ccgggtacgg gccatctgca tgcggcccac ggacggcctg aagcggggcg cggcggtgcg
     7561 caacctaggc cacggcatcc gggtgcccgt tggcaatgcc accctcgggc acgtgtggaa
     7621 cgtcatcggc cgccccctcg atacccgtgg cgccgaggtc gagggcatca cggaatactg
     7681 ggacatccac cggcccccgc cggccttcgc cgacctcgac cccgagacgc ggatgttcga
     7741 gaccggcctc aaggtggtcg acctgctcga gccctaccgg ctcgggggga agatcgggct
     7801 cttcggcggc gccggcgtgg gcaagaccgt gatgatccag gagatgatca tccgggtggc
     7861 gcgccagcac ggcggcgtct ccgtgttcgc cggggtgggc gagcggaccc gcgagggcaa
     7921 cgacctgtgg ctcgagatgc aggagtcggg cgtgatcgcc aacacggcgc tcgtctacgg
     7981 gcagatggac gagccgcccg gcgtccggct gcgcgtcgcc ctgtccgcgc tcaccatggc
     8041 cgagtacttc cgcgacgtgc agaaccagga cgtgctcctt ttcgtcgaca acatcttccg
     8101 gttcacccag gccggctccg aggtgtcgac gctgctcggc cggatgccct cggcggtcgg
     8161 ctaccagccc accctcgccg acgagatggg cgagctccag gagcgcatca cctcggtcaa
     8221 gggtcgctcg atcacctccc tccaggcggt ctacgtgccc gccgacgact acacggaccc
     8281 ggcgccgttc acggccttca cccatttcga cgccaccacc aacctcgacc gggcggtgtt
     8341 cgccaagggc atcttcccgg cggtcgaccc cctcgcctca acgtccaaca tcctccagcc
     8401 gagcatcgtc ggcgaggacc actacaacgt ggcccgccgc atcatccagg tccttcagcg
     8461 ctacaaggag ctccaggaca tcatcgccat cctcggcatc gacgagctgt ccgaggagga
     8521 ccggctgctg gtaggccggg cccggaaggc cgagaagttc ctgggccagc ccatgtacgc
     8581 gggcacgccg ttcaccggga tcgagggcac caacgtgccg gtcgccgaga ctgtggaggc
     8641 gttcgggaat ctgctcgacg gtgagtacga cgacgtgccg gagcaggcgt tcttcaacgt
     8701 cggcggcacg gaggacctca tggccaaggc caagcgcctc agggaggcct gatcgtggct
     8761 atgcaggtcg ccctggtctc gccggagcgc atcctcttcg agggcgaggc cgacatggtc
     8821 gtgggccgga cctcggacgg cgagatcgcg ttcctgaccg gccacgcccc gttcctcggt
     8881 gcgctcggga tcggggcggt caagatcgtc gccaccgacg gccggcagga gcgagccgcc
     8941 gtccacggcg gcttcgtcga ggtccggggc aaccgggtga ccatcctgtc cgacgtcgcc
     9001 gagctggcca gccagatcga cctggagcga gcccgcgccg ccgcggccga cgcggagcgg
     9061 cgcctgatga cggccgacga cgtcgagtgc gaggcccggc tccggcgggc ccacgtgcgc
     9121 atcgccgtcg ccaccgcgac ctagcgccgg ccggccccgc gctggtcgcg gtgccgtcgc
     9181 ctgtgccatc gttccggcga tggctcgagt ccgcctcggc gtggccctgc tcctgccccg
     9241 cgaccaggcc gccgaggtcc aggggttgcg gcgggccctg ggcgatcctg ccctcgatcg
     9301 gatcccgccg cacctgacgc tcgtgccccc ggtgaacgtc cgggccgagg acatgcccgg
     9361 cgcgcttctc cgactgcggg aggcggcggc cgccacggcg ccgttctcgg tggagctggg
     9421 tcctccgtcg accttcctgc ccgacagccc ggtgctgttc ctcgccgtcg acgacgcggc
     9481 cggtgcggtg gcccgcctcc gggagcgggt cttccatccc cccctggcgc ggccgctgac
     9541 ctggccgttc gtgccccaca tgaccctcct ggacggcggc gggcccgggc ggctggaggc
     9601 ggctcggtgg gcgatggccg gcttccgcat cgccgtcgag tgggccgagc tgcacctgct
     9661 ccaggaggag cccggccgga tctggcggtc gttggcctcg gcggtgctcg gcggcagcgc
     9721 cgtcatcggc cgcggagggc tgcctctgga gatcagccgg tccacgcagc cggaccccgc
     9781 ggcccgccgc ttcgcggagc aggcctgggc cgagctcgac ctggccgaga cgggcgggct
     9841 ggcgccgcga gtcgacatgg ccttcaccgc ccggcgctcc ggccaggtgg tcggcgtcgc
     9901 caccgggcgg gccgaggggg gcgtgggtca cctatcggag ctcatcgtgg cctccgccgt
     9961 gcggggcgag ggcgtgggcc accacctgct ggccgccttc gagtcgtggg cggcaggcga
    10021 gggcgtcacc aggctggccc tgcggacgcc ggcggggggc ccggccgagg gcttctacgc
    10081 ccgtcggggg tggcgggagg aggcccggtt gacggactgg tacggcgggc ggtccttcgt
    10141 gcagctccgc cgggacctcg ctccttgagg gtccacctgg gggtcaagcc accgacgccc
    10201 accccgcccc gccgttagct ggggagatta ggtggtctga gaccacctaa tctccccagt
    10261 aacgtgaaag tgcatgagat agttcgttcc aggggggccc ggggtcgcct caggccgcgg
    10321 tgggtctggt ccgtgagcgc gtcaaccgaa ctcgatgatg ctgaactcct ggagcttggg
    10381 caggcggtgc cgggcgtcga tgcgccggac cgtccccgac ttggagcgca tcaccaacga
    10441 ctgcgtgctc gcccccttgc tgtcgtagtg cacgccgcgg agaagctcgc cgtcggtgat
    10501 ccccgtcgcc acgaagaagc agttgtcccc cgccaccagg tcgtcggtgg tcagcacctt
    10561 gctcaggtcg tagccggcgt cgaccgccgt gcgccgctcg tccgcggtcc ggggccagag
    10621 ccggccctgg agctcgccgc ccatgcactt gagggccgcg gccgcgatca ccccctcggg
    10681 ggtgccgccg atgccgaaga ggatgtccgc gcccgagtcc ggccatgcgg tcgagatggc
    10741 gccggccacg tcaccgtcgg ggatcaggcg gatgcgggcg ccggccgccc ggacctcggc
    10801 gatcagggtg tcgtggcggt cgcggtcgag gatcaccgcg gtcacgtcgc gcacagtgga
    10861 gcctttggcc ttggccacgg tctccaggtt ctccgccacc gagcgggtga tgtccaccag
    10921 tcccgccgcc tcgggcccca cggcgagctt ttccatgtag acgcacggcc cggggtcgaa
    10981 catggtgccc cgctcgctga ccgcgatcac cgagagggcg ttgccccggc ccttggcggt
    11041 aagcgtcgtg ccgtcgatcg ggtcgaccgc cacgtcggtc tcgggaggcg agccgttgcc
    11101 gatccgctcc ccgttgtaca gcatcggggc gtcgtccttc tcgccctcgc cgatgacgac
    11161 gacgccgtcc atggcgatgg tctcgagcac cactcgcatg gcgtcgacgg cggcgccgtc
    11221 ggcgccctcc ttgtcgccgc ggcccatcca ccgggaggcc gccagggcgg cgacctcggt
    11281 cacccggacc agctcgaggg ccaggttgcg gtcgggcgcc tgtcgggccg cgttcatggg
    11341 cgccgaccct agtcggcgcg cctcaggggg tgatcttgcc gcgcagccgc tggccgaacc
    11401 aacctcgacc cgagggttcc ccgcctgccg gcggtgctgt tggcgcggtc gtcgaccgcg
    11461 gggacgggcg caaggccgcg agcggctcgc g
//
LOCUS       DASWGG010000089         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_738268, whole genome shotgun sequence.
ACCESSION   DASWGG010000089 DASWGG010000000
VERSION     DASWGG010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738268"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VF954_01590"
     CDS             complement(<1..763)
                     /locus_tag="VF954_01590"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX9529801.1"
                     /translation="MTAAWAGGLRGGLLVCGTHSDAGKSFVVAALCRALARQGVRVAP
                     FKAQNMALNAVVTADGSEIGHAQWLQAHAAGAEPEARMNPVLLKPTGERASQVVVLGR
                     PAGHWSAAEYQRRKPELLATVLDALADLRARFDVVICEGAGSPAEINLLDGDIVNLRL
                     AARAALPAVVVGDIDRGGVFAALYGTVALLPDDLRPLVRGFVVNKLRGDPSLLGGAGR
                     DLEARCGVPTLGVIPMVAGPALDAEDSLALEAWTAG"
     gene            778..1299
                     /gene="cobO"
                     /locus_tag="VF954_01595"
     CDS             778..1299
                     /gene="cobO"
                     /locus_tag="VF954_01595"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX9529802.1"
                     /translation="MARQSIVLVNTGHGKGKSSAAFGVMARGWARGWTVGVVQFVKGG
                     KWKTGERKLADHLGLEWHTMGDGFTWESSDLDRTAEMGRQAWALAAAKLASADYDLLV
                     LDELTYVMKYGWVPVEEVVSGVVGRAARTSVVITGRDAPDELVEVADTVTEMRKVKHA
                     YDRGIGALKGIEY"
     regulatory      complement(1373..>1508)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          204 a          542 c          557 g          205 t
ORIGIN      
        1 tccctgccgt ccaggcttcg agggccaggg agtcctcggc gtcgagcgcc ggccccgcca
       61 ccatgggtat gaccccgagg gtgggcacgc cgcagcgggc ctcgaggtcg cggccggcgc
      121 cgccgagcag gctcgggtcg ccccgcagct tgttgaccac gaagccccgc accagcggcc
      181 gcaggtcgtc gggcagcagg gccaccgtgc cgtacagggc ggcgaacacc ccgccgcggt
      241 cgatgtcgcc caccaccact gcgggcaatg cggcccgcgc cgccagccgc aggttgacga
      301 tgtcgccgtc cagcaggttg atctccgcgg ggctgccggc cccctcgcag atcaccacgt
      361 cgaagcgggc ccgcagatcg gccagggcgt cgagcaccgt ggcgagcagc tcgggcttgc
      421 ggcgctggta ctcggctgcc gaccagtggc cggccggccg gccgagcacg accacctggc
      481 tcgcccgctc tcccgtcggc ttcagcagga ccgggttcat gcgcgcctcg ggctcggccc
      541 cggcggcgtg ggcctggagc cactgggcat ggccgatctc cgacccgtcg gcggtgacga
      601 cggcgttgag ggccatgttc tgcgccttga acggcgccac ccggacgccc tggcgggcga
      661 gggcccggca cagggcggcc accacgaagg acttgccggc gtcgctgtgg gtgccgcaca
      721 cgagcaaccc gccccgtagc ccgcccgccc atgcggctgt cactacgctg ccgccccatg
      781 gctcgccagt cgatcgtcct cgtgaacacc ggccacggca agggcaagtc ctcggctgcc
      841 ttcggcgtca tggcccgggg atgggcgcgg ggctggacgg tgggcgtggt gcagttcgtc
      901 aagggcggca agtggaagac cggcgagcgc aagctcgccg accacctggg cttggaatgg
      961 cacaccatgg gcgacggctt cacgtgggag tcctcggacc tggaccgcac cgcggagatg
     1021 ggccgacagg cgtgggccct ggcggccgcc aagctggcat cggccgacta cgacctgctg
     1081 gtcctcgacg agctgaccta cgtgatgaag tacggctggg taccggtcga ggaggtggtg
     1141 tccggagtgg tcggccgggc ggcgcgcacg agcgtggtga tcaccgggcg ggatgccccc
     1201 gacgagctgg tcgaggtggc cgacacggtg accgagatgc gcaaggtcaa gcacgcctac
     1261 gaccgcggga tcggcgcgct gaaggggatc gagtactagg ccgcgcccct cccggggtgg
     1321 tgagcgccgg cccctgtggt gcatgtgcct ccatcctcgt ggtcgcgagc cggtcggccc
     1381 ctcgagccac ggtcagtctc ctgactcccg gatcagcgct cgccccgcct tcccgggcca
     1441 ggcccagtgg cgtcttgggg ttcgctcccc ggtcacagtg gcgggaccgt accggattcg
     1501 caccggat
//
LOCUS       DASWGG010000090         5274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2671924, whole genome shotgun sequence.
ACCESSION   DASWGG010000090 DASWGG010000000
VERSION     DASWGG010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2671924"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /locus_tag="VF954_01600"
     CDS             complement(<1..1593)
                     /locus_tag="VF954_01600"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX9529803.1"
                     /translation="MADAISVSGPITATDKTLSFETGNLARQSQGAVVASIGDTVVLV
                     TANAAKGVREGIDFFPLTVDVEERMYAAGKIPGSFFRREGRPTDQAILTCRLIDRPLR
                     PSFADGYRNETQVVATIIGADQENPHDVLAINAASAALMLSGIPFDGPIGAVRLAFST
                     EGEWMPHPTYQEGDRSTFELVVAGRQVGDDIAIMMVEAGGTEDAWHYYEAGAPHVTED
                     VIARGLDAAKTWVRESIELQRQLVEQAGRGEPVPFEPQVDYGDDVWGRVSEAGVELLT
                     RAVAIVEKAARNAAIDEATATIAGQLGDDLAGRGGEIKAAVRALTKKLVRKRIVEDGV
                     RMDGRGPTDIRSLSAAVGVIATAHGSGLFERGETQVLNVTTLGMPRMNQLLDTIGIDD
                     SKRYMHHYNMPPYANGETGRVGSPKRREIGHGLLAERALLPVVPSQEEFPYTLRLVSE
                     VLSSNGSTSMASVCSSSLSLMDAGVPIKAPVAGIAMGLVYDGGKYLTLTDILGAEDAF
                     GDMDFKVAGTKDVITALQLDTKL"
     gene            complement(1752..2009)
                     /gene="rpsO"
                     /locus_tag="VF954_01605"
     CDS             complement(1752..2009)
                     /gene="rpsO"
                     /locus_tag="VF954_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX9529804.1"
                     /translation="MPDKAATITTYRLHDSDTGSPEVQVAIFSERINHLTEHLKMHKG
                     DHHTRRGLMKLIGKRRRLLNYVRDNDVERYRELIARLGIRR"
     gene            2100..2531
                     /gene="arfB"
                     /locus_tag="VF954_01610"
     CDS             2100..2531
                     /gene="arfB"
                     /locus_tag="VF954_01610"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX9529805.1"
                     /translation="MAGTEDRHLRVSRSCAVPLDELTWRFSGSGGPGGQHANTSNTRV
                     EVRFDVLGSPSLGPRQRSRLLERLGPTVRVVASDERSQLRNRELALERLRRRLADGLR
                     SEPVRVATRPTAAARRRRLDEKHHQSERKQERARSRRPAED"
     gene            complement(2573..3508)
                     /locus_tag="VF954_01615"
     CDS             complement(2573..3508)
                     /locus_tag="VF954_01615"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX9529806.1"
                     /translation="MEVIRDQASCPRPAGGCVVTIGAYDGVHLGHRALIDEVKQRATH
                     LGSATAVVTFDRHPATVVRPDSAPLVLTDLDQKLELLDSTGIDYALVLTFDDRRAREP
                     AEAFVTGVLVDCLAARAVVVGHDFHFGHNRRGNVQMLTSMGSEYGFDVLGLTLLAQGR
                     EPVSSTRIRRLLADGEVEEAALLLGRPHEVRGRVVAGDGRARDWQFPTANIDVAPDVC
                     LPADGIYAGWYQRPDRTVLAAAISLGTRPTFYERAERSLLEAHLLDFAEDLYGEPARV
                     RFVRRLRREEKFGSEGELVEQIRRDVEETRRVLAR"
     gene            complement(3538..4449)
                     /gene="truB"
                     /locus_tag="VF954_01620"
     CDS             complement(3538..4449)
                     /gene="truB"
                     /locus_tag="VF954_01620"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX9529807.1"
                     /translation="MSSRRAGGAGAALDGLVVVDKEAGWTSHDVVAKCRGVFQQRRAG
                     HAGTLDPDATGVLLVGLGRVTRVLRYLTALPKTYSAEIVLGVATSTLDASGEEAGRWD
                     MGGVTLDAARRAASALTGDILQVPPMVSALKVGGRRLHELAREGIEVDRQPRPVTVHR
                     FDLSAGPEAAVLLATVTCSSGTYVRSLAADLGTALGGGAHLRRLRRVSVGSFGVDRAT
                     PVGGLGAAAVLTPADALRDYPSVTVDAGLAGRVVHGAVLPGDAFGPAPPGAGPRVVLD
                     GSGAALAVYEPAADGGDRLKPSVVLAG"
     gene            complement(4446..>5274)
                     /locus_tag="VF954_01625"
     CDS             complement(4446..>5274)
                     /locus_tag="VF954_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX9529808.1"
                     /translation="KASVASWPEPFSYAPHYRFLPGLELATKPADFPEEPAVMVTFDC
                     GALDRLGSLRATALRAGTLIVLDHHLTNERYGTINVVDPGAAATAVVVRRLASRLGWP
                     LTRDAAICLYTGLVTDTGRFQYGNTTPEVFALAHELSSFGLPLADMTRKLFEEHRFAY
                     LQLVAACLARAELDRELRFVATWVTAADLSAYDVDLDETEGLIDLVRRASEAEVSCVL
                     KETPGGVRVSLRAVSDRDVGALAASFGGGGHRFAAGFTAAGPIERVLGAVRAALAEG"
BASE COUNT          723 a         1884 c         1940 g          727 t
ORIGIN      
        1 cagcttggtg tcgagctgca gggcggtgat gacgtccttc gtgcccgcca ccttgaagtc
       61 catgtcgccg aacgcatcct cggcgcccag gatgtccgtc agggtcaggt acttcccgcc
      121 gtcgtacacc aggcccatgg cgattcccgc gacgggcgcc ttgatgggca cccccgcgtc
      181 catcaacgac agcgacgagc tgcacaccga ggccatcgac gtggagccgt tggacgacag
      241 cacctcgctc accaggcgga gcgtgtaggg gaactcctcc tggctgggga cgacgggcag
      301 cagggcccgc tccgcgagca ggccgtggcc gatctcgcgg cgcttggggc tgccgacccg
      361 gcccgtctcc ccgttggcgt acggaggcat gttgtagtgg tgcatgtagc gcttgctgtc
      421 gtcgatgccg atggtgtcga gcagctggtt catgcggggc atgcccaggg tcgtcacgtt
      481 gagcacctgc gtctcccccc tctcgaacag gcccgagccg tgggcggtgg cgatgacacc
      541 gacggctgcg gacagggacc ggatgtccgt gggcccccgc ccgtccatgc ggaccccgtc
      601 ctcgacgatg cgcttgcgca cgagcttctt ggtcagcgcc cgcacggcgg ccttgatctc
      661 cccgccccga ccggcgaggt cgtcgccgag ctggccggcg atggtcgcgg tcgcctcgtc
      721 gatggccgcg ttgcgggccg ccttctccac gatggccacc gcccgggtga gcagctcgac
      781 ccccgcctcg ctcacccggc cccacacgtc gtcgccgtag tcgacctggg gctcgaaggg
      841 gacgggctcg ccccggccgg cctgctcgac gagctggcgc tggagctcga tcgactcccg
      901 gacccaggtc ttggccgcgt ccaggccccg ggcgatgacg tcctcggtca cgtggggcgc
      961 accggcctcg tagtagtgcc aggcgtcctc ggtaccgccg gcctcgacca tcatgatggc
     1021 gatgtcgtcg cctacctggc gcccggccac caccagctcg aaggtggagc ggtcgccttc
     1081 ctggtaggtg gggtgcggca tccactcgcc ctcggtggag aaggccaggc gaaccgcgcc
     1141 gatcggcccg tcgaagggaa tgccagacag catcagggcg gccgacgccg cgttgatggc
     1201 cagcacgtcg tggggattct cctggtcggc gccgatgatg gtggcgacca cctgggtctc
     1261 gttgcggtac ccgtcggcga aggacggccg aaggggacgg tcgatcagcc ggcacgtgag
     1321 gatggcctgg tcggtgggtc ggccctcccg ccggaagaac gaccccggga tcttgccggc
     1381 ggcgtacatc cgctcctcga cgtcgaccgt gagcgggaag aagtcgattc cctcccgtac
     1441 ccccttggcg gcgttggcgg tcaccaggac gacggtgtcg ccgatggacg cgaccaccgc
     1501 gccctgggac tgccgggcca ggttcccggt ctcgaatgac agtgtcttgt ccgtggcagt
     1561 gatcggcccg gacacggaga tggcatcagc catggtgctc ctttggtcgg ggcggtggcg
     1621 gggccagttc ccagtggtcg aacacccccc aggtgggtac tcggcgactg gcagctgacc
     1681 ggctcgcacc gccgcagcat gcagatatgg accgagcggc ccgggggccg ctcgacagtt
     1741 tggggtaccg cttaccggcg gatgccgagc cgggcgatga gctcccggta gcgctcgacg
     1801 tcgttgtccc ggacgtagtt caagagccgg cgacgcttcc cgatgagctt catgaggccc
     1861 cgccgagtgt ggtggtcgcc cttgtgcatc ttgaggtgct cggtgagatg gttgatccgc
     1921 tcggagaaga tcgccacctg cacctcgggc gacccggtat cggagtcgtg gagacggtag
     1981 gtggtgatcg tggccgcttt gtcgggcatc gggggtgaga acgtcctcgt tgtcacgttt
     2041 tgggtgtcgc cggtttgact ccggccaggg taccaccgcc ggtgagtagc gtctgatccg
     2101 tggccggcac cgaagatcgc catctgcggg tctcgcggtc gtgcgccgtg cccctcgacg
     2161 agctgacgtg gcgcttcagc ggcagcggcg ggccgggcgg ccagcacgcc aacacctcca
     2221 acacccgcgt cgaggtccgc ttcgacgtgc tgggctctcc ctctctgggg ccccgccagc
     2281 ggtcccggct gctggagcgg ctcgggccga cggtccgggt ggtggcatcc gacgagcgct
     2341 cccagctgag gaaccgcgag ctggccctcg agcgcctgcg ccgccggctg gccgacggcc
     2401 tgcggtccga gcccgtgcgg gtcgcgaccc ggccgaccgc ggcggccagg cgtcgccggc
     2461 tcgacgagaa gcaccatcag agcgagcgga agcaggagcg ggcccgctcc cgccggccgg
     2521 ccgaggactg agaacccgcg ggctcaagaa gggacagcgg gtcctggccc gttcatctcg
     2581 cgagcacgcg gcgggtctcc tccacgtcgc ggcggatctg ctcgacgagc tccccctcgg
     2641 agccgaactt ctcctcgcgc cgcagccgcc gcacgaaccg gacccgcgcc ggttccccat
     2701 agaggtcctc ggcgaagtcg agcaggtggg cctcgagcag cgagcgctcg gcgcgctcgt
     2761 agaaggtggg ccgcgtgccg agagagatgg ccgcagccag gacggtccgg tcggggcgct
     2821 ggtaccagcc ggcgtagatc ccgtcggccg gcaggcagac gtcgggcgcc acgtcgatgt
     2881 tggcggtcgg gaactgccag tcccgggccc ggccgtcgcc agccacgacc cggccccgga
     2941 cctcgtgggg ccgccccagc agcagggctg cctcctccac ctcaccgtcg gcgaggagcc
     3001 tgcggatgcg cgtcgaggaa accggctccc ggccctgggc caagagcgta aggccgagca
     3061 cgtcgaagcc gtactcgctc cccatgctcg tgagcatctg gacgttgccc cgccggttgt
     3121 gaccaaagtg gaagtcgtgg ccgacgacca cggcccgggc cgccaggcag tcgacgagga
     3181 cgccggtgac gaaggcctcg gccggctccc gggcccggcg gtcgtcgaag gtgaggacca
     3241 gggcgtagtc gatcccggtg ctgtcgagca gctccagctt ctggtcgagg tccgtcagca
     3301 cgagcggggc ggaatcgggg cgcacgacgg tggccgggtg gcggtcgaag gtcaccacgg
     3361 ctgtggccga cccgaggtgc gtggcccgct gcttcacctc gtcgatgagc gcccggtggc
     3421 ccaggtgcac gccgtcgtat gcgccgatgg tcaccacgca gcccccggcc ggccgcgggc
     3481 acgacgcctg gtcgcggatc acctccatcg cggctgaggc taccgggagg cgcggcctca
     3541 gccggcgagc acgaccgagg gcttgagccg gtcgcccccg tcggcggcgg gctcgtacac
     3601 ggccagggcg gcgccgctgc cgtcgagcac gacccgcggc cccgcgcctg ggggggccgg
     3661 gccgaaggcg tcgcccggca ggaccgcccc gtgcacgacg cggccagcga ggcccgcgtc
     3721 caccgtgacc gacgggtagt cgcgcagggc gtctgccggc gtgagcaccg ccgccgcgcc
     3781 cagtcccccg acgggggtgg cgcggtcgac cccgaaggaa ccgaccgaca cccggcgcag
     3841 gcgccggagg tgagcacccc cgccgagggc ggtgccgagg tcggcggcga gggaccgcac
     3901 gtaggtgccc gacgagcacg tgaccgtggc caggagcacg gcggcctctg gtcccgctga
     3961 gaggtcgaag cggtgaacgg tgacgggcct cggctgccga tccacctcga tgccctcccg
     4021 ggccagctcg tgcagcctcc ttccgccgac cttgagcgcc gacaccatcg gcgggacctg
     4081 gaggatgtcg ccggtgagcg ccgacgcggc ccgccgggcc gcgtcgaggg tgacgcctcc
     4141 catgtcccag cgcccggcct cctcgccgga ggcgtcgagg gtcgacgtgg ccacgccgag
     4201 gacgatctcc gccgagtacg tcttcgggag cgcggtgagg tacctcagca cgcgggtgac
     4261 ccggcccagg ccgacgagga gcacgccggt ggcgtccggg tcgagggtgc cggcgtgccc
     4321 ggcgcgccgc tgctggaaga cgccgcggca cttggccacc acgtcgtgcg aggtccagcc
     4381 cgcctccttg tcgaccacca ccaggccgtc gagcgccgcg ccggcgccgc cggcccggcg
     4441 ggagctcatc cctcggccaa ggcggcgcgc acggcgccga gcacccgctc gatgggcccg
     4501 gccgccgtga acccggccgc gaaccggtgg ccgcccccgc cgaagctggc ggccagggcg
     4561 ccgacgtcgc ggtcgcttac ggcgcgcaac gacacccgca ccccaccggg ggtctccttc
     4621 aggacgcacg acacctcggc ctccgaagcc cgccgcacca ggtcgatcag cccctccgtc
     4681 tcgtccaggt cgacgtcgta ggcggacagg tcggcggccg tgacccacgt ggcgacgaag
     4741 cgcagctcgc ggtcgagctc ggcccgggcc aggcaggcgg ccaccagctg gaggtaggcg
     4801 aagcggtgct cctcgaacag cttgcgggtc atgtcagcca gcggcagccc gaacgagctc
     4861 agctcgtggg cgagggcgaa cacctccggc gtggtgttgc cgtactggaa ccggccggtg
     4921 tcggtgacca gcccggtata gaggcagatg gccgcgtcgc gggtgagcgg ccagcccagg
     4981 cgcgacgcca ggcggcgcac caccacggct gtggccgcgg cgccggggtc caccacgttg
     5041 atcgtgccgt agcgctcgtt ggtcaggtgg tggtcgagca cgatcagcgt cccggcccgg
     5101 agcgcggtcg cacggaggct ccccagccgg tccagcgctc cgcagtcgaa ggtgaccatg
     5161 acggccggct cctccgggaa gtccgccggc ttggtggcca gctccagccc gggcaggaag
     5221 cggtagtggg gggcgtagga gaacggctcg ggccacgacg ccaccgacgc cttg
//
LOCUS       DASWGG010000091         4465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2672053, whole genome shotgun sequence.
ACCESSION   DASWGG010000091 DASWGG010000000
VERSION     DASWGG010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2672053"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /gene="sufD"
                     /locus_tag="VF954_01630"
     CDS             complement(<1..1198)
                     /gene="sufD"
                     /locus_tag="VF954_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX9529809.1"
                     /translation="MAALPGPEWLREARARAWERFASSDRPRADEEVWRYSRIDDFDL
                     GAYVPAAPVPASSPVGRLPDAVEAVGRRSGLIVIHNGWVERAEVDPDLAASGVVVGPL
                     AREPEAPGHWPAGGPDAYVDWAGAFMANGVLVRVPRRVVVPYPIVVVNYIDGDGAAVA
                     GRTHVETGEGAEVTVVEHSGSADVVAVAAPVVVLDAGDGSHLAHLSVQELGRRVWQLG
                     YQTSRTGRDATLQSFAVALGGAYARLRTDARMEGSGATNNLLAAYFGDGDQMHDFRTL
                     QEHVAPKTTSDLLFKGALRDTAHSVYSGLIRVRRGAAGTNAFQTNRNLVLSEGAHADS
                     VPNLEIDENDVSCSHASAVGPIDEEQRYYLESRGIPSDAAERLIVLGFFEDIVGRAPV
                     AGVASVL"
     gene            complement(1391..3898)
                     /gene="lon"
                     /locus_tag="VF954_01635"
     CDS             complement(1391..3898)
                     /gene="lon"
                     /locus_tag="VF954_01635"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX9529810.1"
                     /translation="MAQTNTQALPLLPLSAGVVLPQMVVTLALESDEARRAAEAAVEG
                     DGLLLLVPQVDGRFARVGTVARVESSGELPSGLTGLILRGLHRAVLGAARPGPGSPLW
                     VHADPVFDPAPTERARALAREYRALVDGIGQLRRSRRIGELLAGISEPGALADTAGSW
                     PELALERKVELLEAVDVEQRLDLVVAWAREALGELQIADEIRSNVAEDMEKGQREFLL
                     RQQLAAIRKELGEGAGSEGGDVVAGYRDQVAASLMPADVRQAVEREIDRLERTSEQSP
                     EHGWIRTWLDTLVELPWGVRSDDRLDIAAARAILDRDHTGLDDVKDRVIEHLAVRKLR
                     AERQAERQAERQADGPPQDGPSQDGPSHDRPTSSRRRGDGAILALVGPPGVGKTSLGE
                     SVARALDRSFVRVALGGVRDEAELRGHRRTYVGALPGRIVRAVKEAGTMNPVVLLDEV
                     DKLGSDWRGDPSSALLEILDPAQNHTFRDHYLDVDLDLSDVLFLATANVMENVPAPLL
                     DRMEVVRLDGYTEDEKVAIARDHLLARQVERAGLRPGEVTVTDDALRTIVTDYTREAG
                     VRSLERELGKVLRKVATRLATEQATEQATEQATGQATGQATGPGPGTPAIKVDRGDVR
                     TWLGRARFDIDPEERVYAPGVATGLAVTGLGGDVLSIEATSIEGDGPLALTGQLGDVM
                     KESAEIALSYIRSHATELGVPGDALRGHRFHVHVPAGAVPKDGPSAGVTMTTALTSLL
                     TGRKVRPEVGMTGEVTLQGRVLPIGGVKQKVLAAHRAGLREVVLPVRNGPDLDDVPEA
                     VRQVMTFHLVTDVRQVLDAALEPAPGSAGAVEHRVAA"
     gene            4038..>4465
                     /locus_tag="VF954_01640"
     CDS             4038..>4465
                     /locus_tag="VF954_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX9529811.1"
                     /translation="MFERYSLAAERAARLAQSEARRLGHNMTGPEHLAVGLIADEREP
                     ASAALRSFGLDRDRLRRAAGAVHPPEAPGASVAVPPFNAAAQGLIETARRDALNAGRA
                     GVEPADLLLALLDDDAGVAVLGTAGVDAGRARQAVQRIRS"
BASE COUNT          567 a         1686 c         1623 g          589 t
ORIGIN      
        1 tcagaacgct tgcgactccg gcgacgggag cccggccgac gatgtcctcg aagaagccca
       61 gcacgatgag gcgctcggcc gcgtcactgg ggatgccacg actctccagg tagtagcgct
      121 gctcctcgtc gatgggcccc accgcggagg catggctgca cgacacgtcg ttctcgtcga
      181 tctccaggtt ggggacggaa tcggcgtggg ccccctcgga gagcaccagg ttccggttgg
      241 tctggaaggc gttggtgccg gccgcgccgc gccggacgcg gatgagcccg ctgtagacgg
      301 agtgggccgt gtcccggagc gcgcccttga agagcaggtc gctggtcgtc ttgggggcta
      361 cgtgctcctg gagggtccgg aagtcgtgca tctggtcacc gtcgccgaag tacgcggcca
      421 gcaggttgtt ggtggcgccc gagccctcca tgcgggcgtc ggtgcgcagc cgggcgtagg
      481 cgccgccaag cgccactgcg aaggactgca gggtggcgtc gcggcctgtg cggctggtct
      541 ggtagccgag ctgccagacg cggcgcccga gctcctgcac gctcaggtgg gccaggtggg
      601 acccgtcccc ggcgtcgagc acgacgaccg gcgcggccac agcaacgacg tcggctgacc
      661 cgctgtgctc caccacggtc acctcggcgc cctcgccggt ctccacgtgt gtgcgccccg
      721 ccacggcggc gccgtcgccg tcgatgtagt tgacgacgac gatggggtac ggcaccacga
      781 cccggcgcgg gacccggacg aggacgccgt tagccatgaa cgcgccggcc cagtcgacgt
      841 aggcgtcggg cccgcccgcc ggccagtgcc cgggggcctc cggctccctg gccagtgggc
      901 cgaccaccac ccccgaggcg gccaggtcgg ggtccacctc ggcgcgctcg acccacccgt
      961 tgtggatgac gatcagcccc gagcgccgcc cgacggcctc gaccgcatcg ggcaggcgcc
     1021 ccacagggga ggacgccggc accggcgccg ccgggacgta ggcgccgagg tcgaagtcat
     1081 cgatgcggct gtaacgccac acctcctcgt cggcgcgggg cctgtccgag gacgcgaagc
     1141 gctcccaggc ccgggcccgg gcctcccgca gccactcggg ccccggcagg gccgccaccg
     1201 cgtcggcggt gaagggcgtc agggctggct cccggtgctc atggacctgc tcgatcctcg
     1261 cgaaagaggg gccacgtcgg cccccgaggg ccccactcta ccggggtccg ctcccaatgg
     1321 gcccgtccgg ggccccaacc ccggcctcct ccgcacggcg gggaccccgg cccccccggc
     1381 ggccgtgcgg ctacgccgcg acccgatgct cgaccgcccc ggccgagccc ggggcgggtt
     1441 ccagcgctgc gtccagcacc tggcggacat cggtgaccag atggaacgtc atgacctggc
     1501 ggaccgcctc cgggacgtcg tcgaggtccg ggccgttccg gaccggcagc acgacctccc
     1561 gcaggccggc gcgatgggcg gccagcacct tctgcttcac cccgccgatg ggcaggacgc
     1621 ggccctggag ggtcacctcg ccggtcatcc ccacctcggg ccgcaccttg cgaccggtca
     1681 gcaggctggt cagagcggtg gtcatggtca cgcccgccga gggcccgtcc ttgggcaccg
     1741 cccccgcggg cacgtgcacg tggaagcggt ggccccgaag cgcgtcgccc ggcacgccga
     1801 gctcggtcgc gtgggaccgg atgtaggaga gggcgatctc ggccgactcc ttcatgacgt
     1861 cgccgagctg gccggtcagg gccagagggc cgtcgccttc gatcgaggtg gcctcgatgg
     1921 acagcacgtc cccgcccagc cccgtgacgg ccaggccggt ggccacgccc ggggcgtaga
     1981 cccgctcctc ggggtcgatg tcgaaccggg cccgccccag ccaggtgcgg acgtcacccc
     2041 ggtcgacctt gatcgccggc gtgccaggcc cgggcccggt ggcctgcccg gtggcctgcc
     2101 cggtggcctg ctcggtggcc tgctccgtgg cttgctccgt ggccagcctg gtcgccacct
     2161 tgcgcagcac cttgcccagc tccctctcca gcgagcgcac acccgcctcc cgggtgtagt
     2221 ccgtgacgat cgtccgcagg gcgtcgtcag tcacggtcac ctcgcccggc cgcagcccgg
     2281 cgcgctcgac ctgccgggcc aggagatggt cccgggcgat ggccaccttc tcgtcctccg
     2341 tgtagccgtc gaggcgcacg acctccatgc ggtcgagcag aggcgcgggg acattctcca
     2401 tcacgttggc ggtggccagg aagaggacgt cggacaggtc caggtcgacg tcgaggtagt
     2461 ggtcgcggaa ggtgtggttc tgggccgggt ccaggatctc cagcagcgcc gaggacgggt
     2521 cgccgcgcca gtcgctgccg agcttgtcga cctcgtcgag cagcacgacc gggttcatgg
     2581 tgccggcctc cttcacggcg cgcacgatcc ggccgggcag ggcgccgacg taggtacgcc
     2641 ggtggccgcg cagctcggcc tcgtcgcgga caccacccag ggcgacccgc acgaagctgc
     2701 ggtcgagggc ccgggcgacg gactcgccga gcgatgtctt gcccacgccc ggggggccga
     2761 cgagggcgag gatggccccg tcgccgcggc ggcggctcga cgtaggccgg tcatgagacg
     2821 gcccgtcctg agacggcccg tcctgagggg gcccatccgc ctggcgctcg gcctggcgct
     2881 cggcctggcg ctcggcccgc agcttgcgca cggccaggtg ctcgatgacg cggtccttca
     2941 cgtcgtcgag gccggtgtgg tcacggtcga ggatggcccg ggccgcggca atgtccagcc
     3001 ggtcgtcgga ccggacgccc cacggcagct cgaccagcgt gtcgagccac gtccggatcc
     3061 agccgtgctc cgggctctgc tcgctggtgc gttccagccg gtcgatctcc cgctcgacgg
     3121 cctggcgcac gtcggccggc atcagcgagg cggccacctg gtcgcgatag ccggccacga
     3181 cgtcgccgcc ttcgctgccg gcgccttcgc ccagctcctt gcggatggcc gccagctgct
     3241 ggcgcagcag gaactcgcgc tggcccttct ccatgtcctc ggcgacgttg ctgcggatct
     3301 catccgcgat ctgcagctcg cccagcgcct cgcgcgccca tgcgaccacc aggtccaggc
     3361 gctgctcgac gtcgaccgcc tccagcagct cgaccttgcg ctccagggcc agctcgggcc
     3421 acgagccggc cgtgtccgcc agcgcgccgg gctcgctgat gccggcgagc agctcgccga
     3481 tgcgccggga gcggcgcagc tggccgatgc cgtcgacaag ggcgcggtac tcccgggcca
     3541 gggcccgggc ccgctcggtc ggagccgggt cgaagaccgg gtcggcgtgg acccacagcg
     3601 gcgagcccgg gccgggtcgg gcggcgccga gcacggcccg gtgcaggccg cgcaggatga
     3661 gcccggtgag cccgctcggc agctcgccac tgctctcgac gcgcgccacc gtcccgaccc
     3721 gggcgaagcg gccgtcgacc tgggggacga gcagcagcag gccgtctccc tccacggcgg
     3781 cctcggccgc ccgcctggcc tcgtccgact cgagggcgag ggtcaccacc atctggggca
     3841 gcacgacgcc ggccgacagc ggaagcagcg gaagggcctg ggtgttggtc tgtgccatcg
     3901 ggacctctct ccaaacgggc gcaacgagtt gcggtgtcaa acaggtgcgc ggtgcaacgc
     3961 ccaccccaca tactccattc catccattac accattactg cccttccggg cggggccccc
     4021 ggtctacggt gggcgccatg ttcgagcggt actccctcgc cgcggagcgg gcggcacggc
     4081 tggcccagtc ggaggcccgc cgcctgggtc acaacatgac cggcccagag cacctcgccg
     4141 tcggactgat tgccgacgag cgggagccgg cctcggccgc cctgcgcagc ttcggcctgg
     4201 atcgggaccg gttgcgccgg gcggccggag ccgtccaccc gccagaggcc ccgggggcga
     4261 gcgtcgccgt ccctccgttc aacgcggccg cccaggggtt gatcgagacc gcccgccggg
     4321 acgccctgaa cgccgggcgc gccggagtcg agcccgccga tctcctgctg gccctcctcg
     4381 acgacgacgc cggggtggcc gtgctgggga ccgccggggt agacgccggc cgggctcggc
     4441 aggcggtcca gcgcatccgc agcag
//
LOCUS       DASWGG010000092         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_738665, whole genome shotgun sequence.
ACCESSION   DASWGG010000092 DASWGG010000000
VERSION     DASWGG010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738665"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VF954_01645"
     CDS             complement(<1..231)
                     /locus_tag="VF954_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX9529812.1"
                     /translation="MSALLLGAVAYDPKVVTIWEGFRRWLRRADLGFDYVLYSNYERQ
                     VEDLVDGRIDVAWSSPLAWVRAHRLAPAARALA"
     gene            complement(228..1352)
                     /locus_tag="VF954_01650"
     CDS             complement(228..1352)
                     /locus_tag="VF954_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9529813.1"
                     /translation="MSGYQDALERVIDEVVAPGAAHVDAAGVFPRAAVTALAGAGLLG
                     LTVPPSLGGGGAGLAEAAGVVQRLAGVCGSTAMVVLMHYAGTVLVDNHGPEDVRRAIG
                     RGEHLTTLAFSEAGSRSHFWAPLGTARADAARVGVVRLDARKSWVTAAGEADSYVWSS
                     RPLAADGPMSLWLVPAGSAGLTVGGFDGFGLRGNRSAPVAAAGVTVSADALLGADGTG
                     LDLALAEALPWFLVLSAAFSVGVMEAVTAETGDHVRATRLEHLGQTLAEQPLVRADQA
                     RMRIATDQAAALLGDTLAALAADRPDAMLGVLEVKAAAGEASVAVTDLAMKVCGGAAF
                     RKEVAIERRFRDARAARVMAPTSDALYDFVGRAINGMELF"
     gene            complement(1529..>1738)
                     /locus_tag="VF954_01655"
     CDS             complement(1529..>1738)
                     /locus_tag="VF954_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529814.1"
                     /translation="PYGEQAAGELRRLSGGDRKLAHAAIDQLSELLRRHSRQSKSTAD
                     SEWLTLRTARRLLEESLAHEAYEDV"
BASE COUNT          221 a          666 c          644 g          207 t
ORIGIN      
        1 ggccagggcc cgggccgccg gggccaggcg gtgggcccgg acccaggcga gcggcgagct
       61 ccacgccacg tcgatccgcc cgtcgacgag gtcctcgacc tggcgctcgt agttggagta
      121 cagcacgtag tcgaagccca ggtcggctcg gcgcagccag cgccggaagc cctcccagat
      181 ggtcaccacc ttcgggtcgt aggccaccgc ccccagcagc agcgcgctca aaacagctcc
      241 atcccgttga tggcccggcc cacgaagtcg tacagggcgt cgctcgtggg cgccatgacc
      301 cgggcggccc gggcgtcccg gaagcgccgc tcgatggcga cctccttgcg gaaggcggcc
      361 cccccgcaca ccttcatggc caggtcggtt accgccaccg acgcctcccc ggcggcggcc
      421 ttgacctcca ggacccccag catggcgtcg ggccggtcgg cggccagggc cgccagggtg
      481 tcgccgagca gggctgcggc ctggtcggtg gcgatgcgca tgcgggcctg gtcggcccgc
      541 accaggggct gctcggccag cgtctggccg aggtgctcga gccgggtcgc ccgcacgtgg
      601 tcgccggtct cggccgtgac cgcctccatc acgcccacgc tgaaggccgc gctgagcacc
      661 aggaaccacg gcagcgcctc ggccagggcc aggtccagcc cggtcccgtc ggctcccagc
      721 agggcgtcgg cggagaccgt gacgccggcg gcggccaccg gcgccgagcg gttgccgcgc
      781 agcccgaacc cgtcgaaccc cccaacggtc aatccggccg agcccgccgg caccagccac
      841 aggctcatgg gcccgtccgc ggccaggggg cggctcgacc acacgtagct gtcggcctcg
      901 ccggcggcgg tgacccagct cttgcgggcg tcgaggcgca cgacaccgac gcgggcggca
      961 tcggccctgg ccgttccgag cggcgcccag aagtggctgc gggagccggc ctccgagaac
     1021 gccagcgtgg tgaggtgctc gccccggccg atggcccggc ggacgtcctc gggcccgtgg
     1081 ttgtcgacca gcaccgtgcc ggcgtagtgc atcagcacga ccatggccgt ggagccgcac
     1141 acgccggcca ggcgctggac cacgccggcc gcctcggcga gccccgcgcc gccaccgccg
     1201 agggagggcg ggacggtgag gcccagcagg ccggcgccgg ccagggcggt gacggctgcc
     1261 cggggaaaga cccccgccgc gtccacgtgc gccgcgcccg gcgcgaccac ctcgtcgatg
     1321 acgcgctcca gcgcgtcctg gtaaccgctc actgtcgatc cctcccgttg ctcgatggat
     1381 gtggaaccga cggccgcggc ctgtgcgcta ggggtggcgc gagcggggca cggtcgtcgc
     1441 tgccgcccga ccggacggcg gtctgccgct tcagcgacgg cgtgccacgg cgctgccctc
     1501 cttcaccctc gggaggctag tcgccggatc agacgtcctc gtaggcctcg tgggcgaggg
     1561 actcctcgag cagccggcgg gcggtgcgca gggtgagcca ctcgctgtcg gcggtgctct
     1621 tgctctggcg gctgtggcgc cggaggagct cggaaagctg gtcgatggcc gcgtgggcca
     1681 gcttgcggtc gccgcccgag agacgccgca gctcgcccgc ggcctgctcg ccataagg
//
LOCUS       DASWGG010000093         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2995811, whole genome shotgun sequence.
ACCESSION   DASWGG010000093 DASWGG010000000
VERSION     DASWGG010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2995811"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VF954_01660"
     CDS             <1..1077
                     /locus_tag="VF954_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX9529815.1"
                     /translation="AASHTSYPSLEQQLQTAQAKRSQAAARLNALKATDAQLADAVTT
                     LDRQVKVQAASVDSARVALKAALDAADAADARVASTQAQIGGLRDAVVGRAVAAYTDS
                     TSSLSELLISKDLNAASQRSAFLDEINGRNQDALDSLRAALQDLSGEQGAAAKARQLA
                     SKRRHDAESYLASLAEQMSAKSRLSAALGARIAAQQQESDAMAAQESSIESLIQGQQN
                     AVFAASRGPGGVANGRVSGSGLIWPCSGPITSPFGMRWGRMHEGVDIGCGEGTPIHDA
                     KAGVVIFSGQMSGYGNVIIIDHGGGFSTLYAHESRLSASQGEQVGQGQVIGFVGATGD
                     ATGPHLHFETRVNGTPQNPMQYLP"
     gene            1101..1895
                     /gene="proC"
                     /locus_tag="VF954_01665"
     CDS             1101..1895
                     /gene="proC"
                     /locus_tag="VF954_01665"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX9529816.1"
                     /translation="MTARLVVVGGGKMGQALVRGLLGAGWATAADLAVVEVDAGARER
                     LEGEFPGVAVSAGPVPAEGAVVATKPADVAGACRDLAGTATPRVLSIAAGVPLARLEE
                     WLGPDVPVVRAMPNMPALIGAGAAAIAAGQGATDDDLAWAESILSAVGTVVRVPERLL
                     DAVTGLSGSGPGYVFLVAEALIEGGLLAGLPRDVSRALALQTLLGAARLLVESGESPE
                     ALRAAVTSPGGTTAAGLRALEAAGARAAFLEAVSAATERSRQLGQG"
     gene            2010..2219
                     /locus_tag="VF954_01670"
     CDS             2010..2219
                     /locus_tag="VF954_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9529817.1"
                     /translation="MISLTTQAQPNRPRFLTVGEVADLLRVSNMTVYRLINAGELPAV
                     RVGKSYRLREDDIDKYLADGYTEAG"
     gene            2252..>2605
                     /locus_tag="VF954_01675"
     CDS             2252..>2605
                     /locus_tag="VF954_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX9529818.1"
                     /translation="MSDYGLEDEFVGVVKSVIRGLSPHVAVIDVAHGVPAHDIRAGSL
                     ALVRAVQYLVPGVVLAVVDPGVGTNRRAVAVEVGEGEAVFVGPDNGLLAPAVSMVGGA
                     SRAVALTDPTYHQPAP"
BASE COUNT          347 a          951 c          962 g          345 t
ORIGIN      
        1 gccgcgtcgc atacatccta cccttccctg gagcagcagc ttcagacggc ccaggccaag
       61 cggtcccagg ccgcggcccg gctcaacgcc ctcaaggcga ccgacgccca gctggcggac
      121 gccgtcacga ccctggaccg ccaggtcaag gtccaggcgg ccagcgtcga cagcgcccgg
      181 gtggcgctga aggcggccct ggacgcggcc gacgccgccg atgcccgggt ggcgtcgacc
      241 caggcccaga tcggcgggct ccgtgacgcg gtggtgggcc gggcggtggc ggcctacacc
      301 gattcgacct cctcgctgag cgagctgctc atctccaagg acctcaacgc ggccagccag
      361 cgcagcgcct tcctggacga gatcaacggg cgcaaccagg acgccctgga ctccctccgg
      421 gccgccctcc aggacctctc gggggagcag ggcgcggcgg ccaaggcccg gcagttggcc
      481 tccaaacggc gccatgacgc cgagagctat ctcgccagcc tggccgagca gatgtcggcc
      541 aagagccggc tgtcggctgc gttgggcgcc cgcatcgccg cccagcagca ggagtccgac
      601 gccatggcgg cccaggagtc ctcgatcgag agcctcatcc agggccagca gaacgccgtc
      661 ttcgcggcgt cccgcggccc gggcggggtg gccaacggcc gggtatcggg cagcgggctg
      721 atctggccgt gcagcgggcc catcacctcg ccgttcggca tgcgctgggg ccggatgcac
      781 gagggcgtcg acatcggctg cggcgagggg acgcccatcc acgacgccaa ggccggggtc
      841 gtgatcttct ccggccagat gtcgggctac ggcaacgtga tcatcatcga ccacgggggt
      901 ggcttctcga ccctgtacgc ccacgagtcc aggctcagcg ccagccaggg cgagcaggtc
      961 ggccagggtc aggtcatcgg gttcgtgggc gccaccggag acgccaccgg gccccacctg
     1021 cacttcgaga cgcgggtcaa cggcacgccc cagaacccga tgcagtacct gccctgagga
     1081 agaacccgag gaagaaggag atgaccgctc ggctcgtggt cgtgggcggc ggcaagatgg
     1141 gccaggccct cgtgcggggc ctgttgggcg cgggctgggc gaccgccgcc gacctcgccg
     1201 tcgtcgaggt ggacgccggc gcccgggagc gcctcgaagg cgagttcccc ggtgtcgccg
     1261 tcagcgccgg ccccgtcccc gccgagggcg cggtggtggc caccaagccc gcagacgtgg
     1321 ccggggcgtg ccgcgacctg gccggcacgg ccacgccgcg ggtgctgtcg atcgccgcgg
     1381 gcgttccgct ggcccggctc gaggagtggc tcggcccgga tgtcccggtg gtgcgggcca
     1441 tgcccaacat gcccgccctc atcggggcgg gggcggccgc catcgccgcc ggccagggtg
     1501 cgaccgacga cgacctggcg tgggccgagt ccatcctcag cgcggtcggg accgtcgtgc
     1561 gcgtccccga gcgcctgctc gacgcggtga ccggcctctc cggctcgggt ccgggctacg
     1621 tcttcctggt ggccgaggcc ttgatcgagg gcggcctgct cgccggcctg cctcgcgacg
     1681 tgagccgggc gctcgccctc cagacgctgc tcggcgcggc ccgcctgctg gtcgagtcgg
     1741 gcgagtctcc cgaggcgctc cgggccgcgg tcacctcccc gggcggcacg accgcggccg
     1801 gtctccgtgc cctggaggcg gccggggccc gggccgcctt cctcgaggcg gtgagcgccg
     1861 ccaccgagcg ctcccgtcag ctcgggcagg gctgagcgcc gccgggcacc gggccgagcg
     1921 gggcagggaa cccctccatg cccacggccc ccatttcccc cacttccccc agcaagcact
     1981 gtggcgtaga gtcgtggagg cggagagggg tgatttcact gactacccag gcacagccca
     2041 acaggccgag gttcctcacg gttggggagg ttgccgacct gctacgcgtc tccaacatga
     2101 ccgtgtaccg gctgatcaac gccggtgagc tgcccgcggt gcgggtcggc aagtcctatc
     2161 gcctccggga ggacgacatc gacaagtacc tcgccgacgg gtacaccgag gccggctgac
     2221 cctgcgcccg cgcttcgaca ccgtttcgtt cctgtcggat tacgggctgg aggatgagtt
     2281 cgtcggggtg gtgaaatcgg tcatccgcgg cctgtcaccg cacgttgccg tgatcgacgt
     2341 ggcccacggc gtccccgccc acgacatccg ggccgggagc ctggcgctcg tccgggccgt
     2401 gcagtacctg gtgccgggcg tggtgctggc ggtcgtggac cccggcgtgg gaaccaaccg
     2461 gcgggccgtg gcggtggagg taggggaggg ggaggcggtg ttcgtggggc cggacaacgg
     2521 gctgctggcc cccgccgtct ccatggtggg cggcgcgtcg cgggcggtgg cgcttaccga
     2581 cccgacctac caccagcccg caccc
//
LOCUS       DASWGG010000094         3904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1063915, whole genome shotgun sequence.
ACCESSION   DASWGG010000094 DASWGG010000000
VERSION     DASWGG010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063915"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..1936
                     /locus_tag="VF954_01680"
     CDS             347..1936
                     /locus_tag="VF954_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX9529819.1"
                     /translation="MLQARGLSVEVGGRLTLVEASFTVRAGDKVGLVGRNGAGKTSLL
                     RVLGGEDAAAGGTVLRRGSLGYLPQDPRPEGAGVDATALSHVLSGRGLDEAARRLEKL
                     RLAVEEHPSERNVSRYARAEDAYRDDGGYAADAEVRRIVAGLGLTADRVDAPITVLSG
                     GERRRVELARILFAGSDLLLLDEPTNHLDSDAKAWLMGFLRGYRGALLVVSHDLELLD
                     EAITRVLHLDEGRLVEYKGTYSQYRAARARDEVRLAKLVERQQAEISRLANLADAMRH
                     SSATRARQAKSLDTRVERLRGGAASGPARERRYHVRFPPPPHLGRLALEVDGLAKGYG
                     GPPVLSDVSFAVERGERLLVMGLNGAGKTTLLRLLAGVAEPDAGAIRPGPGASLGYYA
                     QEHEGLVPSRTVLDHLRELSNAPEHELRGLLGMFGLLGEIAFQGAGTLSGGEKTKLAL
                     AQLVVGRHNVLLLDEPSNNLDPPSREAIAGALAGWPGAMILVSHDRDFVAALDPDRVL
                     TLPEAELDYWDDELLELVSLA"
     gene            complement(1958..2485)
                     /locus_tag="VF954_01685"
     CDS             complement(1958..2485)
                     /locus_tag="VF954_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9529820.1"
                     /translation="MARSACPLCGLDEETMAPGDTTATVRSLPRRYGALLRTPGGEED
                     RPDEVARRQPPGGGPSALALTAGLAGLLEATLAAEERLLVADTPEVAVPSEAGAAEPA
                     PADGVETVLGRLEEASRRLGDLLARVPSGDWARAGHRPDGSEVTVLGLARRAAHLGPH
                     LLRQVDATLREVRGR"
     gene            2510..3430
                     /locus_tag="VF954_01690"
     CDS             2510..3430
                     /locus_tag="VF954_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9529821.1"
                     /translation="MAARIGPGVLEIDTMLGGWDRVTAGYLMEGPDPVLIETGSQSSA
                     PVMIEALARLGVGPSDLAGVVVTHIHLDHAGGVGDVARAFPGATVYVHEKGARHLADP
                     SRLVDSAAQVYGPLLDSLYGRLDPTPVERIHVLADGEQIALGPTRTLTAVDSPGHAKH
                     HLGLHDSESGILFVGDAVGVRLPDAGVLRPATPPPDFDLDLAVRSLHRFAARRPAGLA
                     LAHYGLVPDHAAVLAEAEGALRRWAEVAEAAWREGRDIAAALAEAFGADLAGVDSAHR
                     EKLDTLNGVHSNAAGFRRWLETRAAGTGET"
     gene            3434..3871
                     /locus_tag="VF954_01695"
     CDS             3434..3871
                     /locus_tag="VF954_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9529822.1"
                     /translation="MARVEPVRAVVALGLVVSAAAAGQGCGSSSPGSSSNRTAGSGPP
                     AASGRGTPPTDAPGTDDGTVAATVEVKDVAYKPSRVAVTTGQSVTWRFADGDIPHNVN
                     FTTFRSGDPTTTGTYTHTFTTAGRYSYLCDVHPSMVGEVDVTG"
BASE COUNT          488 a         1486 c         1455 g          475 t
ORIGIN      
        1 cgaggccctg gccaaggccg atccggcgtt cctcgacggc ctcgtcagcc ggcgggtccc
       61 gctcgagcgg tggcaggacg cggtcacccg ccagcccggc gacgtcaagg tcgtcatcga
      121 ggtgggggcc tgacgcgccc ggcccgggcc ccacccggca ttcttcaatg ctttaatggc
      181 gtcccagcgt gttttttgcg tgaagaatgg ttttcgtggc gtccgagccc cggtggcgcc
      241 ccggacggcg ccccggtggc gccctgacgg cgccccgacg gcgccccgac ggcgccgggc
      301 cgggaatgct ttcgtggttc ccgccccggc ccgccatcat ggagcggtgc tccaggcccg
      361 cggtctctcg gtggaggtgg gcgggcgcct gaccctggtc gaggcgtcct tcaccgtccg
      421 cgccggggac aaggtggggc tggtgggccg gaacggggcg ggcaagacca gtctgctgcg
      481 cgtgctgggc ggcgaggacg ccgcggccgg cggcaccgtc ctgcgccggg gctccctggg
      541 ctacctgccc caggacccca ggcccgaggg cgccggggtc gacgccaccg ccctgtccca
      601 cgtgctgtcg ggccgggggc tggacgaggc cgcccgccgg ctggagaagc ttcgcctcgc
      661 cgtggaggag cacccgtcgg agcgcaacgt gtcccgctac gcccgcgccg aggacgccta
      721 ccgcgacgac ggcggctacg cggccgacgc cgaggtccgc cgcatcgtcg ccggcctggg
      781 gctcactgcc gaccgggtcg acgccccgat caccgtgctg tccggcgggg agcgccggcg
      841 ggtcgagctg gcccgcatcc tgttcgccgg cagcgacctg ctgctgctcg acgagcccac
      901 caaccacctc gacagcgacg ccaaggcgtg gctcatgggc ttcctccgcg gctaccgagg
      961 cgccctgctc gtcgtcagcc acgacctgga gctgctggac gaggccatca cgcgcgtcct
     1021 gcacctcgac gaggggcgcc tcgtcgagta caagggcacc tactcccagt accgcgccgc
     1081 ccgggccagg gacgaggtcc ggctggccaa gctggtcgag cgccagcagg ccgagatcag
     1141 ccggctggcc aacctggccg acgccatgcg gcactcctcg gccacccggg ccaggcaggc
     1201 caagagcctg gacacgcgcg tggagcgcct gcggggcggc gcggcgagcg ggccggcgcg
     1261 cgagcggcgg taccacgtcc gctttccccc acccccgcac ctcggccggc tcgccctcga
     1321 ggtggacggc ctggccaagg gctacggagg cccgccggtg ctctccgacg tgagcttcgc
     1381 cgtcgagcgg ggcgagcgcc tgctggtgat gggcctcaac ggcgccggca agaccacgct
     1441 gctgcgcctg ctcgccggcg tggccgagcc cgacgcaggt gccatcaggc cggggccggg
     1501 cgcgtcgctc ggctactacg cccaggagca cgagggcctg gtgccgagcc gcaccgtcct
     1561 cgaccacctg cgcgagctgt ccaacgcgcc cgagcacgag ctgcgcggcc tgctcggcat
     1621 gttcggcctc ctgggcgaga tcgccttcca gggggcgggc acgctctccg gcggggagaa
     1681 gaccaagctg gccctggccc agctggtggt cggccgccac aacgtgctgc tgctggacga
     1741 gccgtccaac aacctcgacc cgccctcgcg ggaggccatc gccggcgccc tggcgggctg
     1801 gccgggcgcc atgatcctgg tgtcgcacga ccgcgacttc gtggccgccc tcgaccccga
     1861 ccgcgtgctc accctgcccg aggcggagct ggactactgg gacgacgagc tgttggagct
     1921 ggtgtccctg gcctgacggg cgggggaaag tggggcccta gcggccccgc acctcgcgca
     1981 gcgtggcatc gacctggcgc agcaggtggg gcccgaggtg ggcggcccgc cgggccaggc
     2041 cgagcacggt cacctcggac ccgtcggggc ggtggccggc ccgggcccag tcgcccgacg
     2101 ggacccgggc gagcaggtcg cccagtctcc tcgatgcctc ctccagacgc ccgagcacgg
     2161 tctccacgcc atcggcggga gccggctcgg ccgcccccgc ctcggaaggc acggccacct
     2221 cgggcgtgtc cgccacgagc aggcgctcct cggcggcgag ggtggcctcg agcaacccgg
     2281 ccaggccggc ggtcagggcg agcgcggagg gcccgccccc gggcggttgg cggcgggcca
     2341 cctcgtcggg ccggtcctcc tcgccgccgg gcgtgcgcag cagggcgccg tagcggcggg
     2401 gcagcgaccg gacggtcgcg gtcgtgtcac ccggcgccat cgtctcctcg tcgagaccgc
     2461 agagggggca ggcggagcgg gccatggcct gacactaggg tgcccgtgca tggccgcgag
     2521 gatcggaccc ggagtgctgg agatcgacac catgctcggc gggtgggacc gggtcaccgc
     2581 cggctacctc atggaagggc ccgatcccgt cctgatcgag accggcagcc agagctcggc
     2641 gccggtcatg atcgaggccc tggcccggct cggcgtcggg ccgtccgacc tggccggggt
     2701 ggtggtcacc cacatacacc tggaccacgc cggcggggtc ggcgacgtgg cccgggcctt
     2761 ccccggcgcc accgtgtacg tgcacgagaa gggcgcccgc cacctggccg acccgagccg
     2821 gctggtcgac tcggccgccc aagtctacgg cccgctgctc gactcgctct acgggcgcct
     2881 cgaccccacg ccggtggagc gcatccacgt gctcgccgac ggcgagcaga tcgccctcgg
     2941 gccaacccgc acgctgacgg cggtggactc ccccggccac gccaagcacc acctcgggct
     3001 gcacgactcg gagagcggga tcctgttcgt gggcgacgcg gtcggcgtgc gcctgccgga
     3061 cgctggcgtg ctgcggcccg cgacgccacc gccggacttc gacctcgacc tggccgtgcg
     3121 ctcgctgcat cggttcgcgg cgcgccggcc ggcgggcctg gccctggcgc actacgggct
     3181 ggtgcccgac cacgccgccg tgctggccga ggccgagggc gcactccggc gctgggccga
     3241 ggtggccgag gcggcctggc gggaagggcg cgacatcgcc gccgccctgg ccgaggcgtt
     3301 cggcgccgac ctcgccggcg tcgactcggc gcaccgggag aagctcgaca ccctcaacgg
     3361 ggtgcactcc aacgcggccg ggttccgccg ctggctggag acccgggccg cgggaacggg
     3421 cgagacctga gccatggcac gcgtcgagcc cgtcagggcc gtcgtggcct tgggcctggt
     3481 ggtgtcggca gccgccgctg gccaaggctg cgggtcctcg agccccgggt cctcgagcaa
     3541 caggaccgcg ggctccggcc cgcctgccgc gagcgggcgg ggcacgccgc ccaccgacgc
     3601 gcccggcacg gacgacggca ccgtggccgc cacggtcgag gtcaaggacg tcgcctacaa
     3661 gccgtcgagg gtggccgtga cgaccggcca gtcggtgacc tggaggttcg cagatggtga
     3721 catcccccac aacgtgaact tcaccacctt ccgcagcggc gaccccacga cgaccgggac
     3781 ctacacccac acgttcacca cggcggggcg gtactcctac ctctgcgacg tccaccccag
     3841 catggtgggc gaggtggacg tgaccgggta gccggctggc tccaacccgg gctcgtcctc
     3901 ggga
//
LOCUS       DASWGG010000095         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1064117, whole genome shotgun sequence.
ACCESSION   DASWGG010000095 DASWGG010000000
VERSION     DASWGG010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064117"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VF954_01700"
     CDS             <1..513
                     /locus_tag="VF954_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529823.1"
                     /translation="TPSTDASTTSTALSATALVLSADGLGPLTFGTQAARALSGLTQA
                     LGHAEKVTPVPAGSTCGATRTFQWKNLVVIVNEVNGGSAAKAGLVGWHLGTAAPTALD
                     VRTDKGIGIGSTIAALKAAYGANISIAQGDQGPAVKIAVPNGVITGRVDGLGAANKVQ
                     SLEAGNLCGV"
     gene            603..>1518
                     /locus_tag="VF954_01705"
     CDS             603..>1518
                     /locus_tag="VF954_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX9529824.1"
                     /translation="MTDTQLGQPHAPPGLPPRAVLERMYEVMRTVTVADERARAEAKA
                     GRMPAAFYPVRGLEGVCAALGAVLTADDHLVSTYRNLADTVAKGVPLRAIIAELHGRV
                     PGSSKGKGGTMHMHHDPSGMMATTGIVGSGLPIAAGLALGAQLDGGGRAVAVTFGDGA
                     TSIGAYHEAMNLAALWRLPLVFVCQNNMWAEHTPIAAYAPSTALAERAAAYNLSATAV
                     DGFDPIETWRVLGEAVARARAGGGPTFVECRTYRLVGHTSSADYSYMPPAELDAALAR
                     DPAPTFRAWLVEGDHVAPARLDEIDNAVE"
BASE COUNT          231 a          540 c          549 g          198 t
ORIGIN      
        1 acgccgtcga ccgatgcctc gacgacgagc acggcactca gcgcgaccgc actggtcctg
       61 tcggcggatg ggctcggacc gctcaccttc ggaacgcagg ccgcccgggc actgtcgggc
      121 ttgacgcagg ccctcggcca cgccgagaag gtcacgccgg taccggccgg ctcgacctgt
      181 ggagccaccc ggacgttcca gtggaagaac ctcgtggtca tcgtcaacga ggtgaacggc
      241 ggctccgcgg ccaaggccgg tctcgtcggc tggcatctcg gcaccgccgc tccgacggcc
      301 ctcgatgtgc ggacggacaa gggcatcggg atcggttcga cgatcgcggc cctgaaggcg
      361 gcgtacggcg ccaacatcag catcgcccag ggcgaccagg ggcccgcagt caaaatcgcg
      421 gtgcccaacg gggtcatcac cggccgggtc gacgggctgg gcgccgccaa caaagtccag
      481 agcctcgagg ccggaaacct ctgcggagtg tgagatcagc cgacagctga agcaggctgg
      541 acgtcggcga tagtttctcg atacgatccc tagaaaccac gtttcgaagg gagcgtcgcg
      601 ccgtgaccga cacgcagttg ggtcagcctc acgcgccccc cggtctgccg ccgcgggcgg
      661 tcctggaacg gatgtacgag gtcatgcgca ccgtgaccgt ggccgacgag cgggcccggg
      721 ccgaggccaa ggcggggcgg atgccggccg ccttctatcc ggtgcggggg ctcgaggggg
      781 tgtgcgccgc gctgggggcg gtcctcacgg ccgacgatca tctggtgtcg acgtaccgga
      841 acctggccga cacggtggcc aagggcgtgc ccctgcgggc catcatcgcc gaactgcacg
      901 ggcgggtgcc gggctcgtcc aagggcaagg gcggcaccat gcacatgcac cacgacccgt
      961 cggggatgat ggccaccacc ggcatcgtcg gctccggcct gcccatcgcc gccggcctgg
     1021 cgctgggcgc ccagctcgac ggcggcggac gggcggtggc ggtcaccttc ggggacggag
     1081 ccacctcgat cggcgcctac cacgaggcca tgaacctggc cgccctgtgg cgcctgccgc
     1141 tggtgttcgt gtgccagaac aacatgtggg ccgaacacac gcccatcgcc gcctacgccc
     1201 cgtcgacggc gctggccgag cgggccgcgg cctacaacct gtcggcgacg gcggtcgacg
     1261 ggttcgaccc catcgagacc tggcgggtgc tgggtgaggc ggtggcccgg gcccgggccg
     1321 gcggcgggcc gaccttcgtc gagtgccgca cctaccggct ggtgggccac accagctcgg
     1381 ccgactactc ctacatgccc ccggcggaac tggacgcggc gctggcccgc gaccccgctc
     1441 ccaccttccg ggcctggctg gtggaaggcg accacgtggc gccggcccgg ctcgacgaga
     1501 tcgacaacgc tgtcgagg
//
LOCUS       DASWGG010000096         7552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1064882, whole genome shotgun sequence.
ACCESSION   DASWGG010000096 DASWGG010000000
VERSION     DASWGG010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064882"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VF954_01710"
     CDS             complement(<1..187)
                     /locus_tag="VF954_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX9529825.1"
                     /translation="MAAEEAGPELELRDYLRVLRRRKWTIILAVLVVLGAAITSAELQ
                     TPVYRSGALILLHSSNPQ"
     gene            complement(207..983)
                     /locus_tag="VF954_01715"
     CDS             complement(207..983)
                     /locus_tag="VF954_01715"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX9529826.1"
                     /translation="MVSTAVVAAVGLGAAGCSAARRTARAPEVQFTRAATSPSSTDAS
                     QGITAHGLGRVSGTPDVLTLSIGVDVDAGRATDALSQSSQRAQAVIDSLKQHGVADKD
                     IQTSQLSLWAQYGNGASPVITGYHASDVLTAKLRKLDQAGAAIDAAVASAGNDGRLEG
                     VQFGIDDQSPLKAAARTEAVQRAHDQAAQLAQAAGVKLGALRSLTETAADQGPIMAAS
                     AGTSGRAGVGAPGGVVSAPVPIQPGSQELDVEVVAVYDVG"
     gene            1161..2030
                     /locus_tag="VF954_01720"
     CDS             1161..2030
                     /locus_tag="VF954_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX9529827.1"
                     /translation="MHRHADRSERRDGEWGEWTERSWTSKDALLYALGVGAGAVDPTG
                     AELEFTTENSKDLTQKVLPTFAVVVGAADAGVMRVGTFNFAMLVDGGRAIELHRPIPV
                     EGRIKSRGRVVAVYDKGSGAVIASESESVDVATGEPMFAIRGNVFIRGEGGFGGDRGP
                     SGPRNVAPEREPDHVVTQEVRPDQALLYRLSGDRNPLHSDPQFAAMAGFPKPILHGLC
                     TYGFTGRALLHSLCGSDPARFRSMEARFSKPVFPGERLTVKMWVDDGEAVFRTESSGG
                     EVVIDQGLCTFVP"
     gene            2046..3140
                     /locus_tag="VF954_01725"
     CDS             2046..3140
                     /locus_tag="VF954_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9529828.1"
                     /translation="MPSGAMAPTPDAPGPDAPVPNGAAPDAPGPTDGAPLDEGGEPAL
                     LVAIDRLLFPRSGATYTVEDLAAQAGVSVEVADRLWRAMGFTAGPKDRPFFHGDDLEA
                     LRSAVEALRQGVPLDKIVYQTRVMAAALARVGEVTSDVIVESIEGMRAAGLTEAQIIE
                     ALTSRQQPDFDQVVGYVYRRQLEAALWRKVANPDRLASPELTVGFVDLVRFTAVTEGI
                     ADEELGALVDRFESVVHDAVTDHGGRIVKMIGDEAMFVTDAPADGLEIALSLVDSFAR
                     DESVPPARAGVAHGPVLAHGGDYFGPTVNLSARIVDVARSSTVVVSESLHDALAARAD
                     LTWRRLPPKKLKGIGRVPLWAVTSPPDRRG"
     gene            3204..4160
                     /locus_tag="VF954_01730"
     CDS             3204..4160
                     /locus_tag="VF954_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G1 family glutamic endopeptidase"
                     /protein_id="HEX9529829.1"
                     /translation="MRLRQLVRLGKLGLVAGGAVAVGAAAIGAGGAQAAGPAIVPHAF
                     VPAVSHRPLFSLGANQSSNWSGYNQGALEKNTNFQGVAGDWVVPTATEKVAGEAEYSS
                     TWVGIGGGCIDAGCATTDSTLIQTGTEQDAGNTGGPAYYGWWEIIPAPSFVATDANGN
                     TLPVSAGDAMHATVTQTAPQVWTILLQDTTKGWTFTQTVPYSSSYATAEWIEETPVVI
                     SNSGSVTVGPLPKLSTVHIDNAQTAAAAGGPLAGAGLVPGEEIQLVDPNSGQVLATPS
                     SPDPDADGFNDCTFATTCAAPSGDLGTSGGGGTGHHKLRRAR"
     gene            complement(4179..6197)
                     /locus_tag="VF954_01735"
     CDS             complement(4179..6197)
                     /locus_tag="VF954_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX9529830.1"
                     /translation="MASRAKHWAPTQDARERNLALQCALYVVALGLLSFAAQHHSYVG
                     VVVLTVVLAASGIGVHMTLAAFDRTADARRGQRIAVLLGALEAGVVIVLVRRLQVFDL
                     PGFLGLSLVLFAGGQLAVEVRLSKVLQRPIAAFFGVNLLAVVVGGVLLGKGGWVALAA
                     PAVLLFPTVSLATEDLLDAAAGEAHTARAGRPWSPRAWRIVAGVAAAALVVGMALVVP
                     VGGYAFALVMGALAVGVLAMIAANTDVYVFFVVIAVSLIWSQLPRGAPAPARFGGGHE
                     VVAFGDSYMSGEGASRYLDGTNTNRAATRDECRRSPNSYPVRVARAMHLELDDFACSG
                     AVVANILPSPADVPEPSKNHGQYIGENELQGVRPPPARRLTQLELFDGKHRDPSDVEL
                     VLVMVGGNDAGFADVAQTCIAPGDCSGRGQPRLDRLGTVGATLGPLYDELKRRFGDRV
                     VVVPYPVPIRDQRCWIFNSTMTDSEHRFVSGFAQQLDTVIRAEAAGHHLRYASDMEGA
                     FRVRKERICDRANPDVNYVALNPIQGRISPRDWVHNSMHPKDRGQIVMGDVLSQWLAA
                     AHPPEDPATVPAPVLRLGEELNANPSRPRIEVSDLCAATPPGQPCQPPDATWTYQETR
                     HSLRSHLVAIVAVLGGAWGLCLWGLRLRRHRDAAVRGRKAAPPRSSAP"
     gene            6435..6902
                     /locus_tag="VF954_01740"
     CDS             6435..6902
                     /locus_tag="VF954_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9529831.1"
                     /translation="MYISAKADYATRALLALAAAGGGPVKGEVLAREQGLPLKFLENT
                     LTTLRHAGLVEAQRGSEGGYRLARPAAQITVADIMRPLDGPLAEVRGEKPEEAVYEGP
                     AAHLRDVWVAVRAALREVLENVTLADIVAGELPAGVRELLERPGAWERRRVGG"
     gene            complement(6957..>7552)
                     /locus_tag="VF954_01745"
     CDS             complement(6957..>7552)
                     /locus_tag="VF954_01745"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX9529832.1"
                     /translation="AVLLLDEPLAAVDASARAELRHLLREELRRYPGARLVVTHDPVE
                     AAALAEELVVLEDGRVTQHGPLAEVTARPRSPWVATMVGLNLLAGTAAGTTVRLADGG
                     GAVAIAAPAEGPTFVAIRPNAVSLHRRPPEGSARNVWPGRAGELFLAGDRARVRVEGP
                     VPLIAEVTAAAAAELHLVDGGAVWASVKATDVDTYPM"
BASE COUNT         1027 a         2691 c         2806 g         1028 t
ORIGIN      
        1 tctgcgggtt gctgctgtgc agcaggatca gggcgccgga ccggtacacg ggcgtctgaa
       61 gctcggccga cgtgatggcc gcgccgagca cgaccagcac ggccaggatg atcgtccact
      121 tccgtcggcg caggactcgg aggtagtcgc gcagctccag ctcgggcccg gcttcttcgg
      181 cggccatcgc cggagtctag gcccgctcat ccgacgtcgt agacggccac cacctccacg
      241 tcgagctcct ggctgccggg ctggatgggg accggggccg acacgacccc acccggcgcc
      301 ccgacgccgg ctcggccgct ggtcccggcc gacgccgcca tgatcgggcc ctggtctgcc
      361 gccgtctccg tcagcgagcg cagggcgccg agcttgacgc cggccgcctg ggccagctgg
      421 gcggcctggt cgtgggcccg ttggacggcc tcggtgcgcg cggctgcctt cagcgggctc
      481 tggtcgtcga tgccgaactg caccccctcg agccgcccgt cgttgcccgc cgaggcgacc
      541 gcggcgtcga tggcggcgcc ggcctggtcc agctttctga gtttggccgt cagcacgtcg
      601 gaggcgtggt agccggtgat cacagggctg gcgccgttgc cgtactgcgc ccagagcgac
      661 agctggctcg tctggatgtc cttgtcggcc acgccgtgct gtttgagcga gtcgatcacg
      721 gcctgggcgc gctggctgct ctgggacagc gcgtcggtcg ccctgccggc atcgacgtcc
      781 acgccgatgc tcagggtgag cacgtccggc gtgcccgaga cgcggccgag cccgtgggcg
      841 gtgatcccct ggctggcgtc ggtgctcgac ggcgacgtgg cggcccgggt gaactgcacc
      901 tccggcgccc gggccgtccg gcgggctgcg ctgcatcccg ccgcgccgag gccgaccgcg
      961 gccaccacgg cggttgagac caacagtcct cgtgcgacca tgacgctcct tccggcccac
     1021 cgccaaccgg tggcccacat ggttggacgc cgccgccggt ccgtctggtt cccggccgcg
     1081 gctccgcctt cttgtgacgg gttcttttga cgggttcttg tgacaggttc ttgtgaccgt
     1141 gcgggcccgg cgctgctcca atgcatcgcc atgccgatag atccgagcgc cgtgacggcg
     1201 agtggggtga atggaccgaa cgctcctgga cctcgaagga cgccctgctc tacgccctcg
     1261 gggtcggggc gggcgcggtc gaccccacgg gggccgagct ggagttcacg acggagaact
     1321 ccaaggacct cacccagaag gtcctgccca ccttcgcggt ggtcgtcggg gccgccgacg
     1381 caggggtcat gcgggtcggg accttcaact tcgccatgct ggtggacggg gggcgggcca
     1441 tcgagctgca ccggcccatc ccggtggagg gaaggatcaa gagccgcggc cgcgtcgtcg
     1501 ccgtctacga caagggctcg ggggcggtca tcgcgagcga gtcggagtcg gtggacgtgg
     1561 ccacggggga gcccatgttc gccatcaggg gcaacgtgtt catccgcggg gaaggcggct
     1621 tcgggggaga ccgggggccg agcggacccc gcaacgtggc gccggaacgg gagccggacc
     1681 acgtcgtgac ccaggaggtc cgccccgacc aggccctgct ctaccgcctg tcgggggacc
     1741 gcaaccccct gcattccgat ccgcagttcg ccgccatggc gggattcccc aagcccatcc
     1801 tccacggcct gtgcacgtat gggttcacgg gtcgggcgct ccttcacagc ctctgcggct
     1861 cggacccggc ccggttccgg tccatggagg cccggttctc caagccggtg ttcccgggcg
     1921 agcggctcac ggtcaagatg tgggtcgacg atggcgaggc cgtgttccgc acggagtcga
     1981 gcggcggcga ggtggtcatc gaccaggggc tgtgcacctt cgtgccctga ggcagactgg
     2041 gccgcatgcc gagtggcgcg atggccccga ccccggacgc cccgggcccg gacgcccctg
     2101 tcccgaacgg tgcggccccg gacgccccgg gcccgaccga cggcgccccg ctggatgagg
     2161 gcggcgagcc cgcgctgctg gtcgcgatcg accggctgct gttccctcgc agcggcgcga
     2221 cctacaccgt cgaggatctc gccgcccagg ccggggtgag cgtcgaggtc gccgaccggc
     2281 tctggcgggc catgggcttc acggccgggc ccaaggaccg gcccttcttc cacggtgacg
     2341 acctggaggc cctccgctcg gccgtggaag cgctcaggca gggcgtgccc ctggacaaga
     2401 tcgtgtacca gacgcgggtg atggccgccg ccctggctcg cgtcggcgag gtcacgagcg
     2461 acgtcatcgt ggagagcatc gaggggatgc gagccgcagg tctcaccgag gcccagatca
     2521 tcgaggccct gaccagccgg cagcagcccg acttcgacca ggtggtcggc tacgtctacc
     2581 gccggcagct ggaggcggcc ctgtggcgaa aggtggccaa ccccgatcgg ctcgccagcc
     2641 cggagctgac ggtcggcttc gtggacctgg tccggttcac cgccgtcacc gagggcatcg
     2701 ccgacgagga gctgggcgcg ctggtggacc gcttcgagtc cgtggtccac gacgccgtca
     2761 ccgatcacgg cggccggatc gtgaagatga tcggcgacga ggcgatgttc gtcaccgacg
     2821 ccccggccga cggcctcgag attgccctca gcctggtcga ctccttcgct cgggacgagt
     2881 cggttccgcc cgcccgggcc ggggtggccc acgggccggt gctcgcccac gggggcgact
     2941 acttcgggcc gaccgtcaac ctgtcggcgc gcatcgtcga cgtggcccga tcctcgaccg
     3001 tggtcgtctc cgagtcgctc cacgacgccc tggccgcccg ggccgacctg acgtggcggc
     3061 ggctgccccc caagaagctc aaggggatcg ggcgggtgcc gctgtgggcg gtcacctctc
     3121 cgcccgaccg tcggggttga acgcggttcg cccggggaaa aacgctgccg ttgggatggg
     3181 ctgggtcaac ccaaggaggg ctcgtgcgac tgcggcagct ggtgcgattg gggaagttgg
     3241 gcttggtcgc gggcggagcc gtagccgtcg gggccgcggc catcggggcc ggaggggcgc
     3301 aggcggccgg cccggcaatc gtgccgcatg cgttcgtgcc ggccgtgtcc caccgccccc
     3361 tgttctcgct gggcgccaac cagtcgtcca actggtcggg ctacaaccag ggagccctgg
     3421 agaagaacac gaacttccag ggcgtggccg gcgactgggt ggtgcccacc gccacggaga
     3481 aggtcgccgg cgaggcggag tactcctcga cgtgggtcgg catcggcggc gggtgcatcg
     3541 acgccggctg cgcgaccacc gacagcacgc tgatccagac tggcacggag caggatgccg
     3601 gcaacaccgg cggcccggct tactacgggt ggtgggagat catccccgct ccgtcgttcg
     3661 tcgcgaccga cgccaacggc aacaccctgc ccgtctcggc cggcgacgcc atgcacgcga
     3721 ccgtcacgca gaccgctccg caggtgtgga cgatcctgtt gcaggacacg accaagggct
     3781 ggaccttcac ccagaccgtg ccgtactcct cgagctacgc cacggccgaa tggatcgagg
     3841 agacccccgt ggtgatcagc aacagcggca gcgtcaccgt cgggccgctc ccgaagctgt
     3901 ccaccgtcca catcgacaac gcccagacgg cggccgcggc cggaggcccg ctggcgggcg
     3961 ccggcctcgt gccgggcgag gagatccagc tagtagaccc caactccggg caggtgctgg
     4021 ccacgccgtc gagccccgat ccggacgctg acggcttcaa cgactgcacg ttcgccacga
     4081 cctgcgcggc gccgtccggg gatctgggca cctcaggagg gggagggacc gggcaccaca
     4141 agctgcggcg cgcacgctga gcttctaggc gccgagcttc agggcgccga gcttcggggt
     4201 ggcgcagcct tgcggcctcg taccgcggcg tcgcggtggc ggcgcaggcg cagcccccac
     4261 aggcacaggc cccacgcgcc gccgagcacg gccacgatcg cgaccaggtg ggaccggagg
     4321 ctgtgccggg tctcctggta ggtccaggtg gcgtcgggcg gctggcaggg ctgtcccggt
     4381 ggagtggccg cgcacaggtc gctgacctcg atcctcggcc gggacgggtt ggcgttgagc
     4441 tcctcgccga gcctcagcac cggggccggg accgtcgccg ggtcctcggg cggatgggcg
     4501 gcggcgagcc actggctgag cacgtcgccc atcacgatct ggccccggtc cttcgggtgc
     4561 atgctgttgt ggacccagtc gcgcgggctg atgcggccct ggatggggtt gagggcgacg
     4621 tagttcacgt ccgggttggc ccggtcgcag atccgctcct tgcggaccct gaaggcgccc
     4681 tccatgtccg acgcgtagcg gaggtgatgg ccggccgcct cggcccgtat caccgtgtcg
     4741 agctgctggg cgaagccgga cacgaaccgg tgctcggagt ccgtcatcgt cgagttgaat
     4801 atccaacacc gctggtcgcg gatggggacg gggtagggca cgacgaccac ccggtcgccg
     4861 aagcggcgct tcagctcgtc gtagagcggg cccagggtgg cgccgaccgt gccgagccgg
     4921 tcgagccggg gttggccccg ccccgagcag tcgccggggg cgatgcaggt ctgggccacg
     4981 tcggcgaagc cggcatcgtt gcccccgacc atcaccagca ccagctcgac gtcgctcggg
     5041 tcccggtgct tgccgtcgaa cagctccagc tgggtcagcc gcctggccgg tggcggccgc
     5101 acgccttgca gctcgttctc gccgatgtac tggccgtggt tcttggacgg ctccggcacg
     5161 tccgcgggtg agggcaggat gttggcgacg accgcgcccg agcaggcgaa gtcgtcgagc
     5221 tcgagatgca tggcgcgcgc cacccggacg gggtacgagt tgggtgatcg gcggcactcg
     5281 tcgcgggtcg cggccctgtt ggtgttggtg ccgtcgaggt agcgggacgc tccctctccc
     5341 gacatgtacg agtcgccgaa ggccaccacc tcgtgcccgc cgccgaagcg ggccggcgcg
     5401 ggcgcgccac gcgggagctg cgaccagatg agagacacgg cgatgaccac gaagaagacg
     5461 tacacgtcgg tgttggccgc gatcatcgcc agcaccccga ccgccagcgc ccccatgacg
     5521 agcgcgaacg cgtagccgcc gacgggcacg accagcgcca tgccgacgac cagggccgcg
     5581 gcggccacgc cggcgacgat cctccaggcg cggggcgacc acggccggcc ggcgcgggcg
     5641 gtgtgggcct cgccggccgc ggcgtcgagc aggtcctcgg tcgccaggct gaccgtcggg
     5701 aacagcagga cggccggcgc ggccagcgcc acccaacccc cctttcccag caggacgccg
     5761 cccacgacga cggctaggag gttgacgccg aagaaagctg cgatcggccg ctggagcacc
     5821 ttggacaggc ggacctcgac ggccagctgg cccccggcga acagcaccag cgacaggccg
     5881 aggaaccccg gcaggtcgaa cacctggagc cggcgcacga ggacgatcac gactcccgcc
     5941 tccagcgcgc ccagcaggac ggcgatgcgc tggccccggc gggcgtcggc ggtgcggtcg
     6001 aaggcggcca gcgtcatgtg gacgccgatc cccgaggcgg cgagcacgac cgtcaggacc
     6061 acgaccccga cgtaggagtg gtgctgggcc gcgaaggaga gcaggccgag cgcgaccacg
     6121 tacagggcgc actgcaacgc caggttgcgc tccctggcgt cttgcgtggg cgcccagtgc
     6181 ttcgcccggc tggccacggc gcctcccctc gtccgcccct ccatgggccg ccggcccatt
     6241 cttccatgct ttaatgtcgc cccagcgtgt tttttgcttg aagaatggtt ttggccgcgt
     6301 tccaaactcc ctcagcccgg ccgggtcacc gccggcgagc cagcccaggg cgacgtcgcc
     6361 gagggtgccc tcgcgccgag aacctacccg cccggtcccc gggcacgacc cggcgagcgg
     6421 cgagaatgcg gggcgtgtac atctccgcca aggccgacta cgccaccagg gccctgctgg
     6481 cgctcgccgc cgccggcggc ggtcccgtca agggggaggt gctcgcccgc gagcagggcc
     6541 tgccgctcaa gttcctcgag aacaccctca ccaccctgcg ccacgccggg ctggtggagg
     6601 cccagcgcgg cagtgaaggc ggctaccggc tggcccggcc cgccgcccag atcaccgtcg
     6661 ccgacatcat gcggcccctg gacgggcccc tggccgaggt ccggggcgag aagcccgagg
     6721 aggcggtcta cgagggcccg gccgcccatc tgcgcgacgt gtgggtggcg gtgcgcgccg
     6781 cgctccgcga ggtgctggag aacgttaccc tcgccgacat cgtggctggc gagctgcccg
     6841 cgggggtccg cgagctgctg gagcgtccgg gcgcatggga gcgcaggagg gtcgggggct
     6901 gagggtgaaa gggatcacgc ggtgcgctgt caccgacgtg gccctttcac atcggatcac
     6961 atcgggtagg tgtcgacgtc ggtcgccttg accgaggccc acacggcgcc gccgtcgacc
     7021 aggtggagct cggcggcggc cgcggcggtg acctcggcga tcagcgggac ggggccctcg
     7081 acgcgcaccc gggcccggtc gccggccagg aacagctcgc ccgcccgccc gggccagacg
     7141 ttgcgggcgc tgccctcggg gggtcggcgg tgcagcgaca ccgcgttggg ccggatggcc
     7201 acgaaggtcg gtccctcggc cggcgccgcg atcgcgaccg cgccgccgcc gtcggccagc
     7261 cggacggtgg tgccggccgc cgtgccggcg agcaggttga gcccgaccat ggtggcgacc
     7321 cagggcgagc ggggccgggc ggtgacctcg gcgagcggtc cgtgctgggt gacgcggccg
     7381 tcctcgagga ccacgagctc ctccgcgagg gcggcggcct cgacggggtc gtgggtgacg
     7441 accaggcgcg cgcccgggta gcggcgcagc tcctcccgca gcaggtggcg cagctcggcg
     7501 cgggctgaag cgtctaccgc ggccagcggc tcgtcgagga gcaggacggc gg
//
LOCUS       DASWGG010000097         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1710216, whole genome shotgun sequence.
ACCESSION   DASWGG010000097 DASWGG010000000
VERSION     DASWGG010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710216"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VF954_01750"
     CDS             <1..571
                     /locus_tag="VF954_01750"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX9529833.1"
                     /translation="RSSPARAEIVVHVDWPALVRGYAQGDERCELVGVGPVPVSLVRD
                     LANDCWLKVIVTNGVDVMAVSHGGRNVAAHIRSALEARDPTCVVPGCHVRAGLEIDHW
                     KIPYHQGGPTELWNLCRMCRAHHFDKTHRGARIEGGPGRWVWHPPPSAGSPSRTTPPP
                     PAGARAGPAQPQVLAFEVTGEEARAEAEP"
     gene            complement(602..733)
                     /locus_tag="VF954_01755"
     CDS             complement(602..733)
                     /locus_tag="VF954_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529834.1"
                     /translation="MKAKLSSGLFHPPGAFAYNRTTPDRCYESPEAAEADGLTRTRR"
     gene            1427..>1549
                     /locus_tag="VF954_01760"
     CDS             1427..>1549
                     /locus_tag="VF954_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529835.1"
                     /translation="MADRLIIFDTTLRDGEQAPGISLDVAEKLEIAEQLARLGVD"
BASE COUNT          211 a          544 c          578 g          216 t
ORIGIN      
        1 gcggtcctcg ccggcccggg ccgagatcgt ggtgcacgtc gactggccgg cgctggtgcg
       61 cggctatgcc cagggcgacg agcgctgcga gctggtcggg gtgggaccag tgccggtgtc
      121 gctggtgcga gacctggcca acgattgctg gctcaaggtc atcgtgacca acggggtgga
      181 cgtgatggcg gtcagccacg gcgggcgcaa cgtggcggcc cacatacgct ccgccctcga
      241 agcccgagac cccacctgcg tggtgccggg ctgccacgta cgggcgggcc tggagatcga
      301 ccactggaag atcccctatc accagggtgg gcccacggag ctgtggaacc tctgccggat
      361 gtgccgagcc caccatttcg acaagaccca ccggggcgcc cgcatcgagg gcggtcccgg
      421 ccggtgggtg tggcacccgc cacccagcgc cggctcacct tccaggacaa caccaccccc
      481 gccagccggc gccagggccg gaccggcgca gccccaggtc ctggcgttcg aggtgaccgg
      541 cgaagaggcc cgcgccgaag cggaaccctg agcccgggct cgggcgatgc ttcgtcggcc
      601 tctaccggcg ggtgcgggtc aggccgtcgg cctctgccgc ctcggggctc tcgtagcagc
      661 ggtcaggggt ggtgcggttg taggcgaagg cgccgggcgg gtggaagagc ccgctcgaca
      721 gcttggcttt caccgggtgg gtctccgggc acgccgaccc ggacggctcg acccacctca
      781 cggcaggggc ggccgccttc ttcgccggct tggcggtctt cgcggtcttc gcagccttgg
      841 ccgccttggg tgccttgggt gccgtcctgg ccgaagccgg tggtgaagcg ggcgcgggct
      901 cgggctgggg cgcaaacgcg ggcgcaggct cgggctgggg agcgaccgcg ggcgcgggct
      961 cgggctgggg agggagcgcg cgcgcgggct cgggggaagc gggctcctcc ggtagcggga
     1021 cgtctgcgcc cgcccggacg ggtgcaggcg ggatgggcgg ccagtcgtcg ggccgcgccg
     1081 tcaccggccg ggtggcggcg cggcgagatt ggagcgtccg ggcgatcacg accgccacgc
     1141 cccccaacag tcccagccga agcccaatcc gaaacgaacg gctcatcggt cctcatcctt
     1201 ccgcagcgcg cctgtgagcg tagccagccc gaggggggcc cgtccttgac gggaacccgc
     1261 acagtttggg accatgggcg gtgatgagca tccgtctgct ggccagcatc ctcctgctta
     1321 cctgacggcg agcacctgcc agcgtgctcg ccactcctcc tgcgccctcg ggccaggagg
     1381 ttttttgttg cccgcaaacc gatcacccga ccgaaggacc ccgaccatgg ctgaccgact
     1441 gatcatcttc gacaccaccc tgcgcgatgg cgagcaggcc ccgggcatca gcctcgacgt
     1501 ggccgagaag ctcgagatcg ccgagcagct ggcccgtctc ggcgtcgac
//
LOCUS       DASWGG010000098         8441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_47024, whole genome shotgun sequence.
ACCESSION   DASWGG010000098 DASWGG010000000
VERSION     DASWGG010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47024"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VF954_01765"
     CDS             complement(<1..459)
                     /locus_tag="VF954_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9529836.1"
                     /translation="METLLLERAAGVVTLTLNRPEKKNAVNGAMWREMRQVFDEVAAT
                     VEDRVLVVTGAGGAFCSGADLSDASGGRAMHPLDGVRVINHVALALHRLPKPTIAKVG
                     GVAAGAGCNLAFGCDLVVAADDARFSEIFARRGLTVDFGGTWLLPRLVGLH"
     gene            complement(475..825)
                     /locus_tag="VF954_01770"
     CDS             complement(475..825)
                     /locus_tag="VF954_01770"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX9529837.1"
                     /translation="MTASDRLRLGVEGEAAVAAWYTDRGYEVLARNWRCRDGELDLVL
                     GLGRVFVFCEVKTRSSTAFGLPAEAVTRAKQVRLRRLAARWLDDAAPRRAREIRFDVA
                     SVLAGAIEVIEGAF"
     gene            complement(825..1118)
                     /locus_tag="VF954_01775"
     CDS             complement(825..1118)
                     /locus_tag="VF954_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005056986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HEX9529838.1"
                     /translation="MSAEDLERYETEIELQLYQEYRAVLPMFRYVVETERRFYLANDV
                     KMDAKNEGGRTYFAIELGDAWVWDMYRPARFVSSVRVVTFKDVNVEEIPEKEL"
     gene            complement(1164..1907)
                     /gene="lepB"
                     /locus_tag="VF954_01780"
     CDS             complement(1164..1907)
                     /gene="lepB"
                     /locus_tag="VF954_01780"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9529839.1"
                     /translation="MTVRGPSAIAWAPPVLDEVTSRPEAEGRVGLPAVDDVAGLSPGR
                     IAAEAPVVVVAAVLVAFLVKTFVAQPFYIPSPSMYPQLKINDKVLVSKLAFDLHPPRR
                     GDIVVFDAPPGVEQAVPRRHDNPIVAGLRRVGEGVGLIQPSAEEFVKRVIGLPGETVE
                     AKGGNVYVDGRRLVEPYLPRGTYTSDFGPVTVPAGRLWVMGDNRGDSCDSRCFPRDSG
                     GRAPATIAVGHVVGRAVLKVWPLGHASFL"
     gene            complement(1904..2254)
                     /gene="rplS"
                     /locus_tag="VF954_01785"
     CDS             complement(1904..2254)
                     /gene="rplS"
                     /locus_tag="VF954_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX9529840.1"
                     /translation="MNPTDVIDRASLRDDVPAFAPGDTLKVHVRVVEGTRERLQLFQG
                     VVIRRQGSGVRESFTVRKVSFGVGVERTFPVHSPIIGKIEVVTRGDVRRAKLYYLRDR
                     IGKAAKVKEKRAAR"
     gene            complement(2251..3012)
                     /gene="trmD"
                     /locus_tag="VF954_01790"
     CDS             complement(2251..3012)
                     /gene="trmD"
                     /locus_tag="VF954_01790"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX9529841.1"
                     /translation="MRIDVFTIFPGLIDGFLLESLIGKARTAGIIDVRVHDLRSAATD
                     PHRSVDDAPFGGGAGMVLAPQPLFGAVEAVDPPRPLWLLGPAGRRFQQAMARDLAAGP
                     GFSLLCGRYEGVDARVADHLVDGELSVGDYVLAGGEAAALVVIEAVARLVPGVMGNCD
                     SVSDESFDDGLLEHPQYTRPAQFRAWAVPEVLRSGDHARVARWRRAAALARTLERRPD
                     LIAARGGLTPEEVRLLADHGYPGPSPGGTEEPDAP"
     gene            complement(3018..3473)
                     /gene="rimM"
                     /locus_tag="VF954_01795"
     CDS             complement(3018..3473)
                     /gene="rimM"
                     /locus_tag="VF954_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX9529842.1"
                     /translation="MLEVGRIVRPHGLRGEVVVELVTDRTERLEPGARLYGGKGQPFD
                     VVASRRHQGRWLVTLAGIDDRSAADALRGVVLKAPPLEDPGALWVHQLIGAMVAEAEG
                     AERGKVVAVEANPASDLLVLEDGHLVPLRFVVEATADRLVIDPPPGLFD"
     gene            complement(3478..3789)
                     /locus_tag="VF954_01800"
     CDS             complement(3478..3789)
                     /locus_tag="VF954_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX9529843.1"
                     /translation="MADDSFEEDEDDEEEYEEPATTASPKMSHAVLDYVARSIVDDPD
                     AVEIEAENGRRGVNLRLHVAPDDMGKVIGRRGRVAQAIRTVVRAAGAREGVEAQVDIV
                     D"
     gene            complement(3782..4024)
                     /gene="rpsP"
                     /locus_tag="VF954_01805"
     CDS             complement(3782..4024)
                     /gene="rpsP"
                     /locus_tag="VF954_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX9529844.1"
                     /translation="MKLRLMRMGKTKQPTYRLVAADSRSPRDGRFIEILGTYEPRSEP
                     SVVKIDAGKAQKWLSQGAQPTDTVVKLLKIAGIERG"
     gene            complement(4038..5369)
                     /gene="ffh"
                     /locus_tag="VF954_01810"
     CDS             complement(4038..5369)
                     /gene="ffh"
                     /locus_tag="VF954_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX9529845.1"
                     /translation="MFETLSDRFDAIFTRLRGKGRLGDAEVDEVLREIRLALLEADVS
                     FVVVGEFVKRIRARTVGAELSKSLSPAQQVIKIVHEELAGILGGESLKITYASRPPTT
                     VLLAGLQGSGKTTAAAKLARWFKQQGRHPLVVGADLQRPAAVEQLRVLAGQAGVPMFS
                     EPTDPVAVAAKGLAEAQRVGRDVLIIDTAGRLAIDAELMDEVRQISEKTQPHYTFLVV
                     DAMIGQDAVATAEAFHATLELDGVILTKLDGDARGGAALSVKEVVGRPIAFASIGEKI
                     DDFEVFHPDRMASRILGMGDVLTLIERAEQTYDRDVAEKAAALLHEGQFTLEDFLEQM
                     QQIKKMGPLQGIIGMLPGIPKELKNATIDDRELARVEAIIRSMTPGERRAPTVINGSR
                     RLRIANGSGSSTSEVNLLLKQFSQMQQMMRSMGVTGRMMKKKNAKRKKKGR"
     gene            complement(5524..6699)
                     /gene="ftsY"
                     /locus_tag="VF954_01815"
     CDS             complement(5524..6699)
                     /gene="ftsY"
                     /locus_tag="VF954_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX9529846.1"
                     /translation="MALLLVLLALAVVLLAVSLAAVAVRRRRPEPGPPPAPAPPPAAP
                     AAVPQREEPPSAEPRPEREPEPPPRPEPPPEVAPPEVAPTELAPPEGAVKPRFRDRLG
                     QARALFAGYVSSVLSRMAIDAETWGELEEALIRADVGVATTTRLLEALRARVSADGIK
                     DSQALVDALKADLKETLAAGDRTLHCEPGAPNVWLFVGVNGVGKTTSIGKIAQRERAN
                     GRSVVMAAGDTFRAAATEQLAMWSERAGADLVRGAEGADPGSVIFDAVERARARGADL
                     VLADTAGRLHTKVNLMEELRKVRRIAERPPARLQEVLLVVDATTGQNGLVQARQFTDA
                     VGVTGIVLTKLDGTAKGGIAVAIQADLGLPIKLVGLGETADDLVDFDPSEFVEALFE"
     gene            complement(6727..>8441)
                     /locus_tag="VF954_01820"
     CDS             complement(6727..>8441)
                     /locus_tag="VF954_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529847.1"
                     /translation="PTRPGVGRLLDRLLAGAVAVEGGWPGALDVALAHPGLIVVTREG
                     DRFGPAGWRTGADGSGATAAALEEARHESSEAARVAGAGAAAATDAARRLAEARRAEA
                     ELVEAVAANAAQRAAAGDALQRMHANGRDAAAETETMRADAHEHVERLSRDEARVVEL
                     QAALPGLDAAEAAATGRAASQRSARAHLDQRRAAAAVLRTDLEVRAASLEERRALVTH
                     RLAEVEDRLRDKVEQRSHATRRRQQLEAARLATDRLANVVVDQIGLIEVNLAALRDRR
                     RQAQDAARARTEHLDGLRRSRQASERALGELRERANRAELDQAEVRMRMEAAAEAVRR
                     DLDCEPDAAKAAACPELPEGASAPGRARELERELRLMGPVNPLALEEFTALQERNRFL
                     EGQLEDVKAGRRELAKVIRAVDAEIVEVFAAAYADVAANFEQLFATLFPGGTGKLLLT
                     DPDDLLETGVEVDARPSGKNVRKLSLLSGGERSLTAMAFLFAVFRSRPSPFYLMDEVE
                     AALDDINLHRFLELVHEFRAEAQLVIVSHQKRTMEAADCLYGVTMEPGGSSRVVSERV
                     AARA"
BASE COUNT         1173 a         3088 c         2987 g         1193 t
ORIGIN      
        1 gtgaagcccg accaaccgcg gcagaagcca ggtcccgccg aaatccaccg tgaggccgcg
       61 gcgggcgaag atctcggaga accgggcgtc gtcggccgcc accaccaggt cgcaaccgaa
      121 ggcgaggttg cacccggcgc cggccgccac ccccccgacc ttggcgatcg tcggcttggg
      181 caggcggtgc agcgccaagg cgacatggtt gatgactcgc acgccgtcga gcgggtgcat
      241 ggcccggcca ccggaggcgt cgctcaggtc ggcgccggag cagaaggcgc cgccggcgcc
      301 ggtaacgacc aggacgcggt cctcgaccgt cgcggccacc tcgtcgaaga cctgccgcat
      361 ctcccgccac atggccccgt tgacggcgtt cttcttctcg ggccggttga gggtgagggt
      421 cacgacaccc gcggcgcgct ccagcaatag ggtctccatg gcaagaccat agggctagaa
      481 ggcgccctcg atcacctcga tggcgccggc caggacggac gccacgtcga agcggatctc
      541 cctcgcccgg cggggggcgg cgtcgtccag ccagcgggcc gccaggcggc gcagccgcac
      601 ctgcttggcg cgggtcacgg cctcggccgg cagcccgaag gcggtcgacg accgggtctt
      661 gacctcgcag aagacgaaga cccggcccag acccaggacc agatcgagct cgccgtcgcg
      721 gcagcgccaa ttccgggcca ggacctcgta tccccggtcg gtgtaccagg cagccaccgc
      781 ggcttctccc tcgaccccga ggcggagccg atcgctggcg gtcactacag ctccttttcg
      841 gggatctcct ccacgttgac gtccttgaac gtcaccaccc tcacggagga cacgaaccgg
      901 gcggggcggt acatgtccca aacccacgca tcgccgagct cgatggcgaa gtaggtccgg
      961 cccccctcgt tcttggcgtc catcttgacg tcgttggcca ggtagaaacg gcgctccgtc
     1021 tcgaccacat agcgaaacat gggcaggaca gcccggtact cctgatagag ctgaagctcg
     1081 atctcggtct cgtaccgctc cagatcctca gcgctcatcg acggctcatc gggccaaaac
     1141 catcccctca ccttacgcct gttctacagg aacgaggcat ggcccaatgg ccacaccttg
     1201 agcacggcgc gacccaccac gtggcccacg gcgatggtgg ccggcgcccg cccgccgctg
     1261 tcgcgcggga agcaccggct gtcgcacgag tccccgcgat tgtcgcccat gacccacagc
     1321 cgcccggcgg gaacggtcac gggaccgaaa tcgctcgtgt atgtcccgcg cgggaggtac
     1381 ggctcgacca ggcggcgtcc gtcgacgtac acgttgcccc ccttggcctc gacggtctcg
     1441 cccggcaggc cgatcacgcg tttgacgaac tcctccgcgg acggctggat cagccccacc
     1501 ccctcgccca ccctgcgcag gcctgcgacg atcgggttgt cgtggcgccg ggggacggcc
     1561 tgctcgacgc cagggggcgc gtcgaacacc acgatgtcgc cccgcctcgg cgggtggagg
     1621 tcaaaggcga gcttcgacac gagcaccttg tcgttgatct tcagctgcgg gtacatcgac
     1681 ggagacggga tgtagaaggg ctgggcgacg aacgtcttga ccaggaaggc aaccagcacc
     1741 gcggccacga cgacgaccgg cgcctcggcg gcgatccggc cgggcgagag cccggcgacg
     1801 tcgtcgacgg cgggcaggcc gacccgcccc tcggcctcgg gccggctggt gacctcgtcc
     1861 aggaccggcg gggcccaggc gatcgccgag gggccccgga ccgtcaccgg gcggcccgct
     1921 tctccttgac cttggcggcc ttcccgatac ggtcccggag gtagtacagc ttggcccgcc
     1981 ggacgtcgcc cctggtgacg acctcgatct tgccgatgat gggcgagtgg accgggaagg
     2041 tccgctccac ccccacgccg aagctgacct tccggacggt gaagctctcg cgcacgccac
     2101 tgccctggcg ccggatcacc accccctgga agagctggag gcgctcccgg gttccctcga
     2161 ccacccgcac atgcaccttg agagtgtcgc cgggagcgaa cgcgggcacg tcgtcgcgca
     2221 ggctggcgcg atcgatcacg tcggtggggt tcatggcgcg tcgggctcct cggtaccgcc
     2281 aggggagggc ccaggatagc cgtggtcggc gagcagccgc acctcctccg gcgtgagacc
     2341 gccccgggcg gcgatgaggt cgggccggcg ttcgagcgtg cgggccaggg cggcggcgcg
     2401 gcgccaccgc gccaccctgg catggtcgcc cgagcggagc acctcgggca cggcccacgc
     2461 ccggaactgc gccggccggg tgtactgcgg gtgctcgagc agcccgtcgt cgaagctctc
     2521 gtccgagacc gagtcgcagt tgcccatgac ccccggcacg aggcgcgcca cggcctcgat
     2581 caccaccagg gccgccgcct ctccccccgc caggacgtag tccccgacag acagctcgcc
     2641 gtcgacgagg tggtcggcga cccgagcgtc cacgccctcg tagcggccgc acagcagcga
     2701 gaagcccggg ccggccgcca ggtcccgggc catggcctgc tggaatcgcc ggccggccgg
     2761 cccgagcagc cacagcgggc gcggcgggtc cacggcctcc accgccccga acagcggctg
     2821 cggggccagc accatcccgg ccccgccgcc gaagggggcg tcgtcgaccg accggtgggg
     2881 atcggtcgcc gccgagcgca ggtcgtggac ccggacatcg atgatccccg cggtccgggc
     2941 ctttccgatc aggctctcca ggaggaaccc gtcgatgagg cccggaaaga tggtgaacac
     3001 gtcgatccgc acgggactca gtcgaacagc ccgggcggcg ggtcgatgac cagccggtcc
     3061 gcggtggcct cgactacgaa ccgcagcggt accaggtgcc cgtcctcgag cacgagcagg
     3121 tcgctcgccg ggttggcctc gacggcaacc accttgccac gttcggcacc ctcggcctcg
     3181 gcgaccatgg ccccgatcag ctgatggacc cacagcgcac ccgggtcctc gagcgggggg
     3241 gccttgagca ccacgccgcg cagggcgtcg gcggcgctgc ggtcgtcgat ccccgccagg
     3301 gtcaccagcc accggccttg atggcggcgc gaggccacca cgtcgaaagg ctggcccttt
     3361 cccccgtaca gcctggcgcc gggctcgagc cgctcggtgc ggtccgtgac cagctcgacc
     3421 accacctccc cgcgcaggcc gtgggggcgg acgatgcgcc cgacttcgag caggcgctca
     3481 gtcgacgatg tcgacctggg cctccacgcc ctcccgggcg ccggcggcgc gaacgaccgt
     3541 gcggatggcc tgcgccaccc ggcctcgccg cccgatgacc ttgcccatgt cgtcgggtgc
     3601 gacgtgcagg cgcaggttga cgccccgacg gccgttctcc gcctcgatct ccaccgcgtc
     3661 gggatcgtcg acgatggaac gggccacgta gtcgaggacg gcatgtgaca tcttcggcga
     3721 tgcggtggtc gcgggctcct cgtactcctc ctcgtcgtcc tcgtcctcct cgaaggagtc
     3781 gtcagccacg ctcgatccct gcgatcttga ggagcttgac cacggtgtcg gtcggctggg
     3841 cgccctggga cagccacttc tgcgccttcc cggcgtcgat cttcaccacc gacggctcgg
     3901 accggggctc gtaggtgccc aggatctcga tgaaccgccc gtcccgaggc gagcggctgt
     3961 ccgcggccac gagccggtag gtcggctgct tggtcttccc catccgcatg aggcggagtt
     4021 tcacggccac ttcgtcgtca ccgacccttc ttcttcctct tggcgttctt cttcttcatc
     4081 atccggcccg tcacccccat cgacctcatc atctgctgca tctgcgagaa ctgcttgagc
     4141 agcaggttga cctcggaggt gctggagccg ctgccgttgg cgatccgcag gcggcgggac
     4201 ccgttgatga cggtcggggc tcggcgctcg cccggcgtca tcgaccggat gatggcttcg
     4261 acccgggcca gctctcgatc gtcgatggtg gcgttcttga gctccttggg gatcccgggc
     4321 agcataccga tgatcccctg gagaggcccc atcttcttga tctgctgcat ctgctcgagg
     4381 aagtcctcga gggtgaactg gccctcgtgc aacagcgcgg cggccttctc ggccacgtcc
     4441 cggtcgtagg tctgctcggc ccgttcgatg agggtgagca cgtcgcccat ccccaagatg
     4501 cgcgacgcca tgcggtcggg gtggaagacc tcgaagtcgt cgatcttctc gccgatggac
     4561 gcgaacgcga tgggccggcc cacgacctcc ttcacggaca gggcggcacc gccccgggcg
     4621 tcgccgtcga gcttggtcag gatcacgccg tcgagctcga gggtggcgtg gaaggcctcc
     4681 gcggtggcga ccgcgtcctg gccgatcatg gcgtcgacga cgaggaacgt gtaatgcggc
     4741 tgggtcttct cggagatctg gcggacctcg tccatcagct cggcgtcgat ggcgaggcgg
     4801 ccggccgtgt cgatgatcag cacgtcgcgg ccgacgcgct gggcttcggc gagacccttg
     4861 gccgccaccg ccacgggatc cgtcggctcg gagaacatgg ggacgccggc ctgcccggcc
     4921 agcacccgga gctgctctac ggccgccggt cgttgcaggt cggctcccac gacgaggggg
     4981 tggcggccct gctgcttgaa ccagcgggcc agcttggccg cggccgtcgt cttgcccgag
     5041 ccctggagcc ccgccagcag cacggtggtg gggggtctgg aggcataggt gatcttgagc
     5101 gattcgcccc cgaggatgcc ggccagctcc tcgtggacga tcttgatgac ctgctgggcg
     5161 ggcgacaggc tcttggagag ctcggcgccg acagtgcgcg cccgtatgcg tttgacgaac
     5221 tcgccgacca cgacgaagct gacgtcggcc tccaggaggg cgaggcggat ctcgcgcagc
     5281 acctcgtcga cctcggcgtc gcccagccgt cccttgccac gcagccgcgt gaagatggcg
     5341 tcgaagcgat cggagagggt ctcgaacatg cggctgccca gggtaccggg cggctgccgg
     5401 gcgctcccgc cgtgcggagg aggccggggt tggggccccg gccgggccta ttcgagcacg
     5461 gccgccggcg gggctggggt ccccgccgtg cggaggaggc cggggttggg gccccggccg
     5521 ggcctattcg aagagcgctt ccacgaactc ggaggggtcg aagtcgacga ggtcgtcggc
     5581 cgtctcaccg agtccgacga gcttgatcgg caagcccagg tcggcctgga tggccacggc
     5641 gatgccgccc ttggcggtcc cgtcgagctt ggtcaacacg atgccggtca ccccgaccgc
     5701 gtcggtgaac tgccgggcct ggaccagccc gttctggccc gtggtggcgt ccacgaccag
     5761 cagcacctcc tggaggcggg cgggcgggcg ctctgcgatg cggcgcacct tcctcagctc
     5821 ctccatgagg ttgaccttgg tgtggagccg gccggcggta tcggccagga ccaggtcggc
     5881 gccccgggcc cgggcccgct cgaccgcgtc gaagatgacc gatccggggt ccgcgccctc
     5941 ggcgccgcgg acgaggtcgg caccggctcg ttcggaccac atcgcgagct gctcggtcgc
     6001 cgccgcccgg aaggtgtcgc ccgccgccat cacgaccgat cgtccgttgg cgcgctcgcg
     6061 ctgcgcgatc ttgccgatgc tcgtggtctt gccgacgccg ttcaccccga cgaacagcca
     6121 gacgttggga gcgcccggct cgcagtggag ggtgcggtcg ccggccgcca aggtctcctt
     6181 caggtccgcc ttgagggcgt ccacgagggc ctgggagtcc ttgatcccgt cggcgctaac
     6241 gcgggcgcgc agcgcctcga gcaggcgggt ggtagtggcc accccgacgt cggcccggat
     6301 gagcgcctcc tccagctcgc cccaggtctc ggcgtcgatg gccatacggg agagaaccga
     6361 ggagacgtat ccggcgaaca gggcgcgcgc ctggccgagg cggtcccgga accggggctt
     6421 gaccgccccc tcgggaggcg ccaattcggt tggtgcgacc tcgggcggtg ccacctcggg
     6481 cggcggctcc ggccgcggcg gaggctccgg ctcccgctcc ggtcgcggct cggcggaggg
     6541 cggttcctcc cgttgcggta ccgccgcggg cgcggccggc ggcggggccg gcgcgggggg
     6601 cggtcctggc tctgggcgcc gccggcggac ggctacggca gccagggaca cggccaggag
     6661 aacgaccgcc agcgccagca ggaccaggag gagcgccatg agccgcgcag cctaccgaga
     6721 gcgggctcag gccctggccg ccacccgctc gctgacgacg cgtgaagaac cccccggctc
     6781 catggtcacg ccgtacaggc aatcggccgc ttccatggtg cgcttctgat ggctgacgat
     6841 gacgagctgg gcctcggcgc ggaactcgtg caccagctcg aggaaccggt gcaggttgat
     6901 gtcgtcgagg gccgcctcga cctcgtccat gaggtagaag ggcgagggtc gcgaccggaa
     6961 cacggcgaag aggaacgcca tggccgtcag cgagcgctcg ccgcccgaca gcagcgacag
     7021 cttgcggacg ttcttgccgg acggacgggc gtccacctcg acgccggtct cgagcaggtc
     7081 gtcgggatcg gtcagcaaga gcttgcccgt gccgccaggg aacagcgtgg cgaacagctg
     7141 ctcgaagttg gcggccacat cggcgtaggc cgcggcgaag acctcgacga tctcggcgtc
     7201 aacggcgcgg atcaccttcg ccagctcgcg ccgaccggcc ttgacgtcct cgagctggcc
     7261 ctcgaggaag cggttgcgct cctggagagc ggtgaactcc tccagggcca acgggttcac
     7321 gggccccatg aggcgcagct cccgctcgag ctcgcgggcc cgaccggggg cggacgcccc
     7381 ctccggcaac tccgggcagg ccgccgcctt cgccgcgtcg ggctcgcagt cgaggtcccg
     7441 gcgcaccgct tcggcggcag cctccatccg catgcggacc tcggcctggt cgagctcggc
     7501 gcggttggcg cgctcgcgga gctcgcccag ggcgcgctcc gacgcctggc gtgaccgccg
     7561 caggccgtcc aggtgctcgg tgcgggcccg ggccgcatcc tgggcctgcc gacgacggtc
     7621 ccgcagcgcg gcaagattga cctcgatcaa cccgatctgg tccacgacaa cgttggccaa
     7681 ccggtcggtt gcgaggcggg cggcctccag ctgctggcgt cgccgggtgg catgggatcg
     7741 ctgctcgacc ttgtcgcgga ggcggtcctc cacctcggcc aaccggtggg tcacgagcgc
     7801 tcgccgctcc tccaggctgg cggcccggac ttcgagatcg gtccgcagga cggcggcggc
     7861 ggcgcggcgc tggtccaggt gggcccgggc cgaccgctgg gaggcggcgc gaccggtcgc
     7921 ggcggcctcg gcggcatcga ggccaggaag ggcggcttgc agctcgacga ccctcgcctc
     7981 gtcccggctc agccgctcga cgtgctcatg ggcgtcggcg cgcatcgtct cggtctccgc
     8041 tgcggcgtcc cggccgttcg catgcatgcg ctggagggcg tcgcccgccg cggcccgctg
     8101 ggccgcgttg gcggccactg cctcgacgag ctccgcttcg gcgcgccggg cttcggccag
     8161 gcgccgggcc gcgtccgtag ccgccgcggc gccggccccg gccacccggg cagcctcact
     8221 gctctcgtgc cgcgcctcct ccagggccgc agcggtggcg ccggagccat ccgctccggt
     8281 gcgccatccc gcgggcccga accggtcccc ctcccgggtg acgacgatca gcccgggatg
     8341 ggccagggcc acgtccaggg ctcccggcca gcccccctcc acggcgacgg cgccggcaag
     8401 caagcggtca agcagccggc caaccccagg ccgggtaggg c
//
LOCUS       DASWGG010000099         6610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_742334, whole genome shotgun sequence.
ACCESSION   DASWGG010000099 DASWGG010000000
VERSION     DASWGG010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742334"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VF954_01825"
     CDS             <1..956
                     /locus_tag="VF954_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9529848.1"
                     /translation="DGGVGGGVIDLTVIYQAMGRTLAPTPHLESAVIGAGVLRRGTSQ
                     LARDLLGSVLAGDSIVVPAIAEEDAGFGPDAIALAGTPAAGGGLTLTGTKLLVAYANS
                     ASHFVVAARTSDDAADGLSIAVVARDAPGVSLTRLPNVAGMPLYAVTFDSVPVGADAV
                     IGPAGGGWRLLEPILDGAAVLRSAQIVGASERLLEMSVDYAMQRSQFGQVIGSYQAVQ
                     YLCSDIAINSHLAALHMRYAAGLIDAGEPATVAVSEAKVKANFIARMAPERAHAVHAG
                     VAFVMDFDVQLYTRRCHHWELDLGDDRYHRERIAGQLAGVH"
     gene            981..2477
                     /locus_tag="VF954_01830"
     CDS             981..2477
                     /locus_tag="VF954_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX9529849.1"
                     /translation="MTVTGAPDVSFRSADRFFIGGDWVSPSSPAQFEVIEPATENPLF
                     RIAEAKAEDMARAVGAARQAFDRGPWPRLSHHERAGYLRSFAAGVRARQDDLARVQTG
                     EMGVLYPVAKIVVSHGAAGFDYYADLADNFVFEEHHRPSDGVGAGLLVREPVGVVGAI
                     VAWNGPMPQISYKVAPALLAGCTVVLKASPESPGEAYVLAEIAEEIGLPPGVLNVVTA
                     DREVSELLVRDPRVDKITFTGSTAAGRKIASICGERVARCTLEMGGKSAAIVLDDYDI
                     DLAATVLSRQACSLTGQVCSSLTRVVVGRSRHDELVEALRARFVAVQVGDPFDPATGM
                     GPLATRRQRDRVEGYIAKGVAEGATLATGGRRPAHLDRGFFIEPTIFADVDNASTIAQ
                     EEIFGPVTSVIAADSEDQAVEIANGTNYGLASAVFTNDVEHAYRVARQIRAGTVGHNA
                     PRADFGIAFGGFKQSGIGREGGSEGLLPFLETKTILLAGEPTPPKGDA"
     gene            2474..3271
                     /locus_tag="VF954_01835"
     CDS             2474..3271
                     /locus_tag="VF954_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9529850.1"
                     /translation="MSFDPPASLKYETRGGICYITINRPEAMNALNLEVQGALPLAIE
                     EFESDLSLYVGVITGEGGRAFSAGGDLKEMATYVGTDDNPMRRAGRAARPGIQAGGAR
                     KPLIAAIDGYAFAGGCELALGCDIRIATRKSQFAIPEVKRSIIAGPGITHLPRMIPQG
                     EALLMALTGDPITAERAYQVGLIQGLAEDRDELFEKVDKIAATIANNPPLAVQDVKRI
                     VKLGVNMPIEYAVAFRDKYSDVILQTEDAKEGPAAFAEKRAPVWKSR"
     gene            complement(3308..5443)
                     /locus_tag="VF954_01840"
     CDS             complement(3308..5443)
                     /locus_tag="VF954_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX9529851.1"
                     /translation="MELHARLTGVDASPNPVRARLARLLRPKTIAFVGLSDNRVAFLD
                     TIAPTFDSDAEIFVVNPNYPTVLDRPTVPTLSALDRPVDAVMSFLSAERTTQLVEEAA
                     GLDVGGVVLVAAGFAELDSGGEALQERIKAAAAATSMPVIGPNGLGFVNVPAKVSLTI
                     AAPHKRRAGGISVVSQSGAMLSGIAMAAWDYDRVGTNILVSAGNEAITDLADYVDFLA
                     EDDDTKAIALVIEQIRRPAEFFAAVARATEAGKPVAALKLARNERSQRMAASHTGALT
                     GDAWVYDVALQQAGVALAYDPEELIDRLALADQLPRRRWTPVSSLGVLTVSGGFASLS
                     LDLALGHGLNVPPLDGYKEWVSTSMPGITVPNPLDATGVGARIWPEIVDKYVTDPELD
                     AILVIHLMADEDGDRGHISRTLVQEFAKSAQKVDKPCVVANCSGTPAPWVRELLGDSL
                     VFGRGVRSSLRGLATLGAFVRYRDGRRQAPPSGPEIARPASTPIPQPEGLMLPFVDTM
                     ALLAEHGIPVAPCHMVAADADPSGVRPPFAGPYVVKLADVAHRTEHDAVRLKVDDAGL
                     PDAIGGLRKIAAADGLSPLVVVQPMLEITGETLLGIQASSELGPLVVFGLGGIFVEAL
                     NKIGGRMAPFDAHEARALIEEFTGVKVMHGFRGQPPWDLEALSELLVAAGRLAANGRK
                     WISTLDVNPLVYGPTGFHAVDALLLVRPG"
     gene            complement(5422..>6610)
                     /locus_tag="VF954_01845"
     CDS             complement(5422..>6610)
                     /locus_tag="VF954_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529852.1"
                     /translation="LALRKAIEDAGVEPSEVDGLVMTPDVTTGARWPVGEPAPPEVVS
                     AFEQTADPFDGITKLSPEWILKNMPELTNIKLVMTPGICMSMALPAAIQAVGDGLTNT
                     CLVLRGWLNLDGRYYAGGPNGEPAVAGPGRFGSSQAGPACFDTAQQFQRYMHKYGKPH
                     EMMAPFIVNSKKNGLLFPEGYFAQHRPADVTAEDYVSSRWVSEPANLFDNDLPIHSST
                     AYLVTTAERAKDMKQKPAYVLGHAGAGVVKGESYSRVRAHGVIENLEEIEDNAAATGR
                     KIFEASGVTAKDLDFENLYDGFALFHVFHIEGIGYAGIKRGEALDLFQTDISIHGPNP
                     VSPSGGNVGSGRTRCWKWTDSIQQIQGRAGARQISKPANIGISGGFIPSWSNFVVWSS
                     TPG"
BASE COUNT          971 a         2315 c         2371 g          953 t
ORIGIN      
        1 aggacggcgg ggtcggaggc ggcgtcatcg acctcaccgt catctaccag gccatggggc
       61 gcacgctggc gcccacgccg cacctcgagt cggccgtgat cggagcgggc gtgctccgcc
      121 ggggcacctc ccagctcgcg agggacctgc tggggagcgt gctcgccggc gactcgatcg
      181 tcgtgcccgc catcgccgag gaggacgccg ggttcggccc cgacgccatc gccctcgccg
      241 gcaccccagc ggccggcggc ggcctgaccc tgaccggcac caagctgctg gtcgcctacg
      301 ccaacagcgc cagccacttc gtggtggccg cccgcacgtc cgacgacgcc gccgacggcc
      361 tgtcgatcgc cgtggtggcc agggacgcac ccggcgtgtc gctgacccgc ctgccgaacg
      421 tggcgggcat gccgctgtat gccgtcacct tcgactcggt gccggtcggt gccgacgccg
      481 tcatcggccc cgccggcggc gggtggcggt tgctcgagcc catcctggac ggggccgcgg
      541 tgctccgctc ggcgcagatc gtcggggcca gcgagcggct gctcgagatg tcggtcgact
      601 acgcgatgca gcgatcacag ttcggtcagg tcatcggcag ctaccaggcc gtgcagtacc
      661 tgtgctccga catcgccatc aacagccacc tagcggcgct gcacatgcgg tacgcggccg
      721 ggctgatcga cgcgggagag ccggcgacgg tggcggtgtc tgaggccaag gtgaaggcca
      781 acttcatcgc ccggatggcg cccgagcgcg cccacgccgt gcacgccggc gtggcgttcg
      841 tgatggactt cgacgtgcag ctctacaccc gccggtgcca ccactgggag ctcgacctgg
      901 gtgacgaccg gtaccaccgc gagcggatcg cggggcagct cgccggggtc cactgaccgg
      961 agcgaggaga aggagtcgac atgaccgtta ccggcgcacc tgacgtttct ttccgcagcg
     1021 cagaccgctt cttcatcggc ggcgattggg tcagcccgtc gtcgcccgcc cagttcgagg
     1081 tgatcgagcc ggctaccgag aacccgctgt tccggatcgc cgaggccaag gccgaggaca
     1141 tggcgcgagc ggtcggggcg gcgcgccagg ccttcgaccg cggtccctgg ccgcggctga
     1201 gccaccacga gcgggccggg tacctgcgct ccttcgccgc cggggtgcga gcccgccagg
     1261 acgacctggc ccgggtgcag accggcgaga tgggcgtgct gtacccggtg gccaagatcg
     1321 tggtctcgca cggcgccgcc gggttcgact actacgccga cctggccgac aacttcgtgt
     1381 tcgaggagca ccaccggccc agcgacggcg tcggtgccgg gctgctcgtg cgcgagccgg
     1441 tcggcgtggt cggcgccatc gtggcgtgga acggccccat gccacagatc agctacaagg
     1501 tggcgccggc gttgctggcg ggctgcacgg tggtgctgaa ggcatccccg gagtcaccgg
     1561 gcgaggccta cgtcctcgcc gagatcgccg aggagatcgg cctgccgccg ggcgtgctca
     1621 acgtggtcac ggccgaccgc gaggtgtcgg agctgctggt gcgcgacccg cgggtggaca
     1681 agatcacctt caccggatcg accgcggccg ggcgaaagat cgcctccatc tgcggcgagc
     1741 gcgtggcccg gtgcaccctg gagatgggcg ggaagtcagc cgccatcgtg ctcgacgact
     1801 acgacatcga cctcgccgcc acggtcctga gccggcaggc ctgctcgctc acgggccagg
     1861 tgtgctcgtc gctcacgcgc gtggtggtgg gccgctcccg ccacgacgag ctagtcgagg
     1921 ccctccgggc ccggttcgtc gccgtccagg tcggcgaccc gttcgatccg gccacgggga
     1981 tgggcccgtt ggccacccgg cgccagcgcg accgggtcga gggctacatc gccaaaggcg
     2041 tcgccgaggg ggcgacgttg gccaccggag gacgccgccc ggcgcatctc gaccgcgggt
     2101 tcttcatcga gccgaccatc ttcgccgacg tggacaacgc ctcgaccatc gcccaggagg
     2161 agatcttcgg cccggtgacc tccgtgatcg ccgcggacag cgaggaccag gccgtggaga
     2221 tcgccaacgg caccaactac gggctggcat cagcggtgtt caccaacgac gtcgagcacg
     2281 cctaccgcgt ggcccgccag atccgggcgg gcaccgtcgg ccacaacgcc ccccgcgccg
     2341 acttcggaat cgcattcggc gggttcaagc agtccggcat cgggcgggaa ggcggctccg
     2401 agggcctgct tcccttcctc gagaccaaga cgatcctgct cgccggagag cccacccccc
     2461 caaagggaga cgcatgagct tcgatccgcc ggcatcgctg aagtacgaga cgcggggcgg
     2521 catctgctac atcaccatca accgcccgga ggccatgaac gcgctgaacc tcgaggtgca
     2581 gggcgcgctg cccctggcga tcgaggagtt cgagtccgac ctgtcgctgt acgtcggcgt
     2641 gatcaccggt gagggcggtc gggccttctc cgccggcggc gacctcaagg agatggccac
     2701 ctacgtcggc accgacgaca acccgatgcg ccgcgccggc cgcgccgcgc gcccgggcat
     2761 ccaggcggga ggcgcccgca agcctttgat cgcggcgatc gacgggtatg ccttcgcggg
     2821 aggctgcgag ctagcgctgg gctgcgacat ccgcatcgcg acccgcaagt cgcagttcgc
     2881 catcccggag gtcaagcgca gcatcatcgc cggcccgggc ataacccacc tgccgcgcat
     2941 gatcccgcag ggcgaggcgt tgctgatggc gctgaccggg gaccccatca ccgccgagcg
     3001 cgcctaccag gtcggcctga tccagggcct ggccgaggac cgcgacgagc ttttcgagaa
     3061 ggtcgacaag atcgccgcca ccatcgccaa caacccgccc ctcgccgtcc aggacgtcaa
     3121 gcggatcgtg aagctcggcg tcaacatgcc aatcgagtat gccgtcgcct tccgggacaa
     3181 gtactccgac gtcatcctcc agaccgagga cgccaaggag ggcccggccg cgttcgcaga
     3241 gaagcgcgcc ccggtctgga agtcgcgctg aaccctcagg acgcccctca ggacgccgct
     3301 caggtcgtca gccgggccgc accagcagca gggcgtcgac cgcgtggaac ccggtagggc
     3361 cgtacacgag cgggttgacg tccagcgtcg agatccactt cctgccgttc gccgccagcc
     3421 ggcccgccgc caccagcagc tcgctcagcg cttcgaggtc ccagggcggc tgcccgcgga
     3481 agccgtgcat gaccttgacg cccgtgaact cctcgatgag cgccctggcc tcgtgcgcgt
     3541 cgaagggcgc catgcgcccg ccgatcttgt tgagcgcctc gacgaagatg ccaccgaggc
     3601 cgaacaccac caacggcccc agctcggagc tggcctggat gccgagcaga gtctcgccgg
     3661 tgatctccag catgggctgg accacgacca acggcgagag cccgtcggcg gcggcgatct
     3721 tgcgcaaccc gccgatggcg tcgggcaggc cggcgtcgtc gaccttcagg cgcacggcgt
     3781 cgtgctcggt gcggtgggcc acgtcggcca gcttcaccac gtagggcccg gcgaacggcg
     3841 ggcggacgcc ggacgggtcg gcgtcggcgg ccaccatgtg gcagggcgcc acggggatgc
     3901 cgtgctcggc gagcagcgcc atggtgtcga cgaagggcag catcagcccc tcgggctggg
     3961 ggatcggcgt gctcgccggg cgggcgatct cggggcccga cggcggggcc tggcggcggc
     4021 cgtcgcggta gcggacgaag gcacccaggg tggccaggcc gcgcaggctg gaacgcacgc
     4081 cccggccgaa cacgagcgag tcgcccagca gctcccgcac ccacggggcc ggggtcccgg
     4141 agcagttggc gaccacacac ggcttgtcga ccttctgggc ggacttggcg aactcctgga
     4201 cgagggtgcg gctgatgtgg ccccggtcgc cgtcctcgtc cgccatcagg tggatcacca
     4261 ggatggcatc cagctccggg tcggtgacgt acttgtcgac gatctcgggc cagatgcggg
     4321 cgccgacgcc ggtcgcgtcc agcgggttgg gcacggtgat gccgggcatg ctcgtgctga
     4381 cccactcctt gtagccatcg agcgggggga cgttgaggcc gtggccgaga gccaggtcca
     4441 ggctcagcga ggcgaagccc ccgctcaccg tcagcacgcc gagcgacgag accggggtcc
     4501 agcggcggcg gggcagctgg tcggccaggg cgaggcggtc gatgagctcc tccgggtcgt
     4561 aggcgagcgc aacgccggcc tgctgcagtg ccacgtcgta cacccacgcg tccccggtca
     4621 gcgcgccggt gtgagaggcg gccatgcgct gggagcgctc gttgcgagcc agcttgagcg
     4681 cggcgacggg cttgccggcc tcggtggcgc gcgccaccgc ggcgaagaac tccgccggcc
     4741 ggcggatctg ctcgatcacc agggcgatcg ccttggtgtc gtcgtcctcg gcgaggaagt
     4801 cgacgtagtc ggcgaggtcg gtgatggcct cgttgccggc cgagaccagg atgttcgtgc
     4861 cgacccggtc gtagtcccag gccgccatgg cgatgccgct cagcatggcc ccgctctggg
     4921 agaccaccga gatgccgccg gctcgccgct tgtggggggc ggcgatggtc aggctgacct
     4981 tcgccggcac gttgacgaag cccaggccgt tgggcccgat caccggcatc gaggtcgccg
     5041 ccgcggccgc cttgatgcgc tcctggaggg cctcgccgcc ggagtcgagc tcggcgaagc
     5101 cggccgccac caggaccacg ccaccgacgt cgagccccgc cgcctcctcg accagctggg
     5161 tggtgcgctc ggccgagagg aagctcatca ccgcgtccac cgggcggtcg agcgccgaca
     5221 gcgtgggcac ggtcggccgg tcgaggaccg tcggatagtt ggggttgacg acgaagatct
     5281 cggcgtcgct gtcaaaggtg ggtgcgatgg tgtcgagaaa ggcgacccgg ttgtcgctca
     5341 ggccgacgaa cgcgatcgtc ttcggccgca gcagtcgggc caggcgggcg cggaccgggt
     5401 tgggtgaggc gtcgacgccg gtcaacctgg cgtggagctc caaacgacga agttgctcca
     5461 cgacgggatg aacccgccgg agatcccgat gttggccggc ttgctgatct ggcgggcgcc
     5521 cgcccgcccc tggatctgct ggatcgagtc ggtccacttc cagcagcggg tgcgcccgct
     5581 tccgacgttg ccgccgctcg gggacaccgg gttggggccg tggatcgaga tgtcggtctg
     5641 gaacaggtcc agcgcctcgc cccgcttgat cccggcatag ccgatgccct ctatgtggaa
     5701 cacgtggaac agcgcgaacc cgtcgtagag gttctcgaag tccaggtcct tggctgtgac
     5761 gccggacgcc tcgaagatct tcctcccggt ggcagccgcg ttgtcctcga tctcctcgag
     5821 gttctcgatc acgccgtgcg cccggactcg cgagtaggac tcgcccttca caacgccggc
     5881 gccggcgtgg ccgaggacgt aggccggctt ctgcttcatg tccttggcgc gctcggcggt
     5941 ggtgaccagg taggccgtcg acgagtggat cggcaggtcg ttgtcgaaca ggttcgccgg
     6001 ctcggagacc caccgcgagc tgacgtagtc ctcggcggtg acatccgccg ggcgatgctg
     6061 ggcaaagtat ccctcgggga acagcaggcc gttcttcttc gagttgacga tgaacggcgc
     6121 catcatctcg tggggcttgc cgtacttgtg catgtagcgc tggaactgct gggcggtgtc
     6181 gaaacaggcg gggccggcct gcgagctgcc gaatcggccg gggccggcca ccgccggctc
     6241 cccgttgggg ccgcccgcgt agtagcggcc gtcgaggttg agccagcccc gcagcaccag
     6301 gcaggtgttc gtaagcccgt cgccgacggc ctggatggcg gcgggcagcg ccatggacat
     6361 gcagatcccc ggcgtcatca cgagcttgat gttggtgagc tccggcatgt tcttgaggat
     6421 ccactccggg ctcagcttgg tgatgccgtc gaagggatcg gcggtctgct cgaaggcgga
     6481 caccacctcg ggcggagctg gctctcccac cggccagcgc gccccggtcg tgacgtcggg
     6541 cgtcatgacc aggccgtcga cctcgctggg ctcgaccccg gcgtcctcga tcgccttgcg
     6601 cagcgccagg
//
LOCUS       DASWGG010000100         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_369995, whole genome shotgun sequence.
ACCESSION   DASWGG010000100 DASWGG010000000
VERSION     DASWGG010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369995"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="VF954_01850"
     CDS             complement(<1..1334)
                     /locus_tag="VF954_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX9529853.1"
                     /translation="MAGAAFFDLDRTLLHGASGPLLSRALVDAGISPDRRIPGQDLLY
                     RFYEVVGETLPSMALARGAALAARGWSAEAVRKAAEEVAEQLETMVAPFARPLLKEHR
                     AKGRPTVLATTTPHDLVAPLARRLGFDDVVATRYDERDGSYTGRLAGEFVWATGKLAA
                     VRRWAATQDVAMGASFAYSDSIYDLPLLSAVGHPHAVNPDVRLHAVAVLRRWPVLHLD
                     VPPGVPKLAGLEPFDLLRLVVRPELFPYARFDIDGAELVPEDGGAILASNHRSYFDVA
                     ALALTAVRAGRALRFLGKREVFDAPVIGPLARSLGGIPVDRDTTSGGPLRSAALAVEG
                     GEVVAVLPQGTIPRGRAFFEPELKAKSGAARLAAMTGAPVVPVGLWGTEAVWPRRSRL
                     PNVTNLLHPPSVTVRVGGVVQLAYDDPRADTAAIMRAITALLPEAARQPVTPT"
     gene            1555..2469
                     /locus_tag="VF954_01855"
     CDS             1555..2469
                     /locus_tag="VF954_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9529854.1"
                     /translation="MDLRQLQAVLAVADHGTFSAAADALHTVQSNVSTHVARLERELG
                     VTLIDRAAGGLTEEGELVVQRGRRVMAELEALVVDVAALRHEVQGTARLGMIGTIARW
                     LAPQLLDVMATRHPNVHLVIVEGTSNLLEPQLSSGRLDMAVLNLPVPSEDLVTALLFD
                     EDLVLVVPTDDPLARRGRLVLADVATLDLLLPPAGTAFRDELDEATRPAGVVLRAKTE
                     LDGTWLIASLTFEGHGPAILPASAVPGYMRDRWALVNVEGFPRRHVGVAQRRKGLLSA
                     PARATLEVLNELVQGGGTDLPPGIHLRP"
     gene            2484..>3404
                     /locus_tag="VF954_01860"
     CDS             2484..>3404
                     /locus_tag="VF954_01860"
                     /inference="COORDINATES: protein motif:HMM:NF000689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX9529855.1"
                     /translation="MYDAVLLVSFGGPEGPDDVVPFLRRVAAGRAVPDERLAEVAEHY
                     HRFGGVSPINGANRALKAAIEDLLRRDGPPLPVYWGNRNWHPLLEDTVAAMAGDGVRR
                     AVAFVTSAYSSYSSCRQYLEDIERARGAAGAGAPRIDKLRPFYNHPGFIGPFARATGA
                     ALEELGQRGGDAHLVFSAHSIPLAMAETSEYRRQLIQSSELVAGAVARSSGRDHPWRL
                     VFQSRSGPPSVPWLEPDVNSALAELAGAGAQAAVIVPIGFVSDHLEVVYDLGVVALGT
                     ARRLGINAVRAATPGTDPEFVAMARELILER"
BASE COUNT          452 a         1234 c         1253 g          465 t
ORIGIN      
        1 gtcggcgtca ccggctggcg ggcggcttct ggcagaaggg cggtgatggc gcgcatgatg
       61 gccgcggtgt cggcccgggg gtcgtcgtag gccagttgga cgacgccgcc gacgcgcact
      121 gtcacactcg gcgggtgcag caggttggtg acgttgggca gccgggaccg gcggggccac
      181 acggcctcgg tgccccacag gcccaccggg acgactgggg cgcccgtcat cgcggccagc
      241 cgtgccgcgc cgctcttggc cttcagctcg ggctcgaaga acgcccggcc acgcgggatg
      301 gtgccctggg gcaggacggc gaccacctcg ccgccctcga cggcgagggc ggcggagcgc
      361 agcggcccgc ccgacgtggt gtcgcgatcg acggggatcc cgcccaggga ccgggcgaga
      421 gggccgatga ccggtgcatc gaagacctcc cttttcccca ggaaccgaag ggcgcggccg
      481 gcccgcaccg ccgtcaacgc cagggccgcg acgtcgaagt agctgcggtg gttggacgcc
      541 agtatggcgc ctccgtcctc ggggaccagc tcggcgccgt cgatgtcgaa gcgggcgtag
      601 gggaatagct cggggcgcac gaccaggcgc agcaggtcga agggctccag gccggcgagc
      661 ttgggcaccc cgggcggcac gtcgaggtgc agcacgggcc agcgtcgcag cacggccacg
      721 gcgtgcagcc ggacgtcggg gttcacggcg tgggggtggc cgacggccga gagcaggggg
      781 aggtcgtaga tgctgtcgga gtaggcgaaa ctggcaccca tggcgacgtc ctgggtcgcg
      841 gcccatctcc gcaccgcggc cagcttgccg gtcgcccaca cgaactcgcc ggccagcctg
      901 ccggtgtagc tgccgtctcg ctcgtcgtag cgtgtggcca ccacgtcgtc gaagcccagt
      961 ctccgcgcca acggcgccac caggtcgtgc ggtgtggtcg tggccagaac ggtgggccgc
     1021 cccttggccc ggtgctcctt tagcagtggc cgggcgaagg gggcgaccat cgtctcgagc
     1081 tgctcggcca cctcctctgc cgccttccgg acggcctccg ccgaccaccc ccgggcggcc
     1141 agggcagccc cccgggccag cgccatggac ggcagggtct cgccgacgac ctcgtagaac
     1201 ctgtacagca ggtcctggcc cgggatgcgc cggtcgggcg agatgccggc atccaccagc
     1261 gcccggctga gcagcgggcc gctggcgccg tggagcaggg tgcggtccag atcgaagaag
     1321 gcggccccgg ccattgcccc tcaaagtagt agggcacctc acggtaatgg ttggcggccc
     1381 cgaacgggcc gggacgcgac gaagggaccg gcgctggggg aggccggccc cttcgttgga
     1441 cctcgtgcga ccactggtgg ggggattacc gtgccgcggg tctgctattc agtatgcggg
     1501 tcgccggcct atctgtccaa cagttgtgac ggatagccga tatcttgttt ggcgatggat
     1561 cttcgccagc tgcaggccgt gctggcggtg gccgaccacg gcacgttctc cgccgcagcc
     1621 gacgccctcc acacggtgca gtccaacgtc tcgacccacg tggcccgcct cgagcgggag
     1681 ctgggcgtca ccttgatcga ccgggcggcg ggtggcctca cggaggaggg cgagctggtg
     1741 gtccaacggg gccgccgggt gatggcggag ctcgaggcgc tggtcgtcga cgtggcggcg
     1801 ctgcgccacg aggtccaggg caccgcccgc ctgggcatga tcggcaccat cgcccgctgg
     1861 ctggcccccc agctcctcga cgtgatggcc acccggcacc ccaacgtcca cctcgtgatc
     1921 gtggagggga cgagcaacct cctcgagccc cagctgtcgt caggccggct cgacatggcc
     1981 gtgctcaacc tgccggtgcc ctccgaggac ctcgtgaccg ccctgctgtt cgacgaggac
     2041 ctcgtgctgg tggtgcccac cgacgatccg ctcgcccgcc ggggccggct cgtcctcgcc
     2101 gacgtggcga ccctcgacct actgctcccg cccgccggca cggcgttccg ggacgagctg
     2161 gacgaggcca cccggccggc cggggtcgtg ctgcgggcca agaccgagct ggacggcacc
     2221 tggctcatcg cctccctgac cttcgagggc cacggcccgg ccatcctccc cgcctccgca
     2281 gtgccgggct acatgcggga ccggtgggcc ctggtcaacg tcgagggctt cccgcgccgc
     2341 cacgtcggcg tggcccagcg gcgcaaggga ctcctctcgg ctccggcccg ggccaccctc
     2401 gaggtgctca acgagctggt gcagggcggc gggaccgacc taccgccggg catccacctg
     2461 cgcccatgat ggaagatgcg ggcatgtacg acgccgtcct gctggtgtcc ttcggcggtc
     2521 ccgagggccc ggacgacgtg gtcccgttcc tccgccgggt ggcggccggg cgggcggtgc
     2581 cggacgagcg gttggcggag gtggccgagc attaccaccg cttcgggggg gtcagcccta
     2641 tcaacggcgc caaccgggcc ctgaaggccg caatcgagga cctcctccgt cgagacggac
     2701 ctcccctgcc cgtgtactgg ggcaaccgca actggcaccc cctgctcgag gacacggtgg
     2761 ccgccatggc cggcgacggc gtccggcggg ccgtggcgtt cgtcacgtcg gcgtacagct
     2821 cctactcgag ctgccgccag tacctcgagg acatcgagcg ggcccgcggc gcggccgggg
     2881 ccggcgcgcc ccgcatcgac aagctgcggc ccttctacaa ccacccgggc ttcatcggcc
     2941 ccttcgcgcg cgccaccggc gcggccctgg aggagctcgg gcagaggggc ggggacgccc
     3001 acctggtgtt cagcgcccat tccatcccgt tggccatggc cgagacgagc gagtaccggc
     3061 gccagctcat ccagagcagc gagctggtag ccggcgccgt cgcccggagc tcaggccgcg
     3121 accacccgtg gcgcctggtc ttccagagcc gcagcggccc gccctcggtg ccgtggctgg
     3181 aaccggacgt caactccgcg ctggccgagc tggccggggc cggcgcgcag gcagccgtca
     3241 tcgtgccgat cggcttcgtg tccgaccacc tggaggtggt gtacgacctc ggcgtggtgg
     3301 cgctcggcac cgctcgccgg ctgggcatca atgccgtgcg agcggccacg cccgggaccg
     3361 acccggagtt cgtggccatg gcccgcgagc tgatcctcga gcgc
//
LOCUS       DASWGG010000101         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1068685, whole genome shotgun sequence.
ACCESSION   DASWGG010000101 DASWGG010000000
VERSION     DASWGG010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068685"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VF954_01865"
     CDS             <1..517
                     /locus_tag="VF954_01865"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX9529856.1"
                     /translation="DGPALAARVRRLPWVADARVGRSWPSTVRIWVSERKAVAVVAAA
                     GRGVAAVDRTGRVLATGAQTFPGLPLVSGIAPAGPPGTATDAAAQPGLAVAQALSPGL
                     AGRVAQVAVEADGTVTVHLAGGGPVVRLGSPDGLAEKLQALDTILTRVDLRGVNTVDV
                     RVPRAPVLTRQ"
     gene            680..1756
                     /gene="ftsZ"
                     /locus_tag="VF954_01870"
     CDS             680..1756
                     /gene="ftsZ"
                     /locus_tag="VF954_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX9529857.1"
                     /translation="MAGPTQTYLAVIKVVGIGGGGVNAVNRMIDAGLKGVEFIAVNTD
                     AQALLMSDADVKLDIGRQLTRGLGAGADPEVGRQAAEEHRDEIEEVLKGADMVFITAG
                     KGGGTGTGGAPVIAEIAKALGALTIGVVTRPFSFEGRRRAVQAEQGIQRLREKVDTLI
                     VIPNDRLLTVSNDKTSMVNAFKMADEVLLQGVQGITDLITTPGVINTDFADVKAIMNN
                     AGTAIMGIGAASGEGRAVNAARAAITSPLLEASIEGARGILLNIAGGSDLGLFEVNEA
                     AEIIHGVAHPDANIIFGQVIDDAMGDEVRVTVIAAGFERWDEGGRAPEAGLGLGATGG
                     REMFGGDEDLELGDDDFDVPSFLK"
     gene            1761..2474
                     /locus_tag="VF954_01875"
     CDS             1761..2474
                     /locus_tag="VF954_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEX9529858.1"
                     /translation="MAQGSAIWRAPAGIVARWTGRAEGDLGHGGRYVSTVDPDVEGRR
                     RAVLDRSWSWVRQVHGAAVAVADRPGGGAGTVADALVSARADTALAVLTADCAPVALA
                     SPEGVLAAAHGGWRGLLAGVIERTVEAMSGLGATHIVAAVGPCVRAECYEFGVEDLDQ
                     VAARLGRSVRSVTADGKPALDLVAAVAATLQRAGVSDVDLTSAACTACCPDYFSHRAR
                     GEAQRQALVVWRRPPGSHG"
     gene            2467..>2982
                     /locus_tag="VF954_01880"
     CDS             2467..>2982
                     /locus_tag="VF954_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529859.1"
                     /translation="MGDPRAALTAGAVAERLAVVRTRIAAAGGDPGRVRVVAVTKGFG
                     PAAVEAAAGAGLFDIGENYAQELGGKVVATGHLAALRWHFLGPLQRNKVARLAQVVAV
                     WQSVDRRAAVDAIAAASPGASVFIQVNLAEDPNRPGCSWAAVGDLVRRGQGAGLDVQG
                     LMGVAPLGHGSG"
BASE COUNT          395 a         1054 c         1124 g          409 t
ORIGIN      
        1 cgacgggccc gccctcgcgg cccgggtccg gcgcctgccc tgggtcgccg acgcccgggt
       61 agggcgatcg tggccgtcga cggtccggat ctgggtcagc gagcggaaag ccgtcgccgt
      121 ggtggccgcg gcgggccgcg gcgtggccgc cgtcgaccgc accgggcggg tgctggcaac
      181 cggcgcgcag acctttcccg gtcttccgct cgtgtccggc atcgctcccg ccgggccgcc
      241 cggaacggcg accgacgccg cggcccagcc cgggctggcg gtggcccagg ccctgagccc
      301 cggcctggcg ggacgggtgg cgcaggtggc ggtggaggcg gacgggaccg tgaccgtcca
      361 ccttgccggc gggggcccgg tggtccggct cggttcgccg gacgggctgg ccgagaagct
      421 ccaggccctc gacaccatcc tgacccgcgt cgacctccgg ggagtgaaca ccgtggatgt
      481 gcgggttcct cgggcgccgg tcttgacccg ccaatgacca ctcccgtaca gtttgaacca
      541 acccctgagg ttgacataac tctaacccta aaccttaggt tgagcctggg cgccggcccg
      601 ggctgctccg gctctcgtcc cgtctgcggg ggggcgccgg cgcgccgcac ccacccacaa
      661 ccggaggcat gagcgtccta tggccggacc gacccagact tacctcgccg tcatcaaggt
      721 ggtcggcatc ggtgggggcg gcgtcaatgc cgtcaaccgc atgatcgacg ccggcctgaa
      781 gggcgtcgag ttcatcgcgg tgaacaccga cgcccaggct ctgctcatga gcgacgcaga
      841 cgtgaagctc gacatagggc ggcagttgac ccggggcctc ggcgcgggcg cggacccgga
      901 ggtcggccgg caggcggcgg aggagcaccg ggacgagatc gaggaggtgc tgaagggcgc
      961 ggacatggtc ttcatcaccg ctggcaaggg tggcggcacc ggtaccggcg gcgcccccgt
     1021 gatcgccgag atcgccaagg cccttggtgc cctcaccatc ggcgtggtca cgcggccgtt
     1081 ctcgttcgag gggcggcggc gcgccgtgca ggccgagcag ggcatccagc gcctgcggga
     1141 gaaggtcgac accctcatcg tcatccccaa cgaccggctc ctcaccgtgt ccaacgacaa
     1201 gacgtccatg gtcaatgcct tcaagatggc ggacgaggtc ctcctgcagg gtgtccaggg
     1261 catcaccgac ctcatcacca ccccgggcgt gatcaacacc gacttcgcag acgtcaaggc
     1321 gatcatgaac aacgccggta ccgcgatcat gggcatcggt gcggcgtcgg gtgaggggcg
     1381 ggccgtgaac gcggcacgag ccgccataac cagcccgttg ctcgaagcct cgatcgaagg
     1441 ggcgcgcggc atcctgctga acatcgccgg cggcagtgac ctcggcctgt tcgaggtcaa
     1501 cgaggccgcc gagatcatcc acggcgtcgc ccatcccgac gccaacatca tcttcggcca
     1561 ggtgatagac gacgccatgg gcgacgaggt ccgggtcacg gtgatcgctg ccgggttcga
     1621 gcgctgggac gagggtggcc gggctcccga ggccggcttg gggttgggcg ccacgggcgg
     1681 ccgcgagatg ttcggcggcg acgaggatct agagctcggc gacgacgact tcgacgtccc
     1741 atcgttcctc aagtaggccg gtggcccagg ggtcggcgat ctggagggcc ccggccggca
     1801 tcgtggcccg ctggacgggg agggccgagg gcgacctcgg gcacggaggc cggtacgtat
     1861 cgaccgtgga tcctgacgtg gagggccgga ggcgcgccgt gctcgaccgg agctggtcgt
     1921 gggtcaggca ggtccacggc gcggccgtcg ccgtggcgga ccgcccgggc ggcggggcgg
     1981 gcacggtcgc ggacgctctg gtgagcgcgc gagccgacac cgccctggcg gttctgaccg
     2041 cggactgcgc ccctgtggcc ctggccagcc ccgagggcgt cctggccgcg gcccacggag
     2101 gctggcgcgg cctgctcgcc ggcgtcatcg agcgcaccgt cgaggccatg tccggcctcg
     2161 gcgccacgca catcgtcgcc gcggtgggcc cctgcgtccg cgccgagtgc tacgagttcg
     2221 gggtcgagga cctcgaccag gtcgcggcca ggctcggtcg ctccgtgcgc tcggtcaccg
     2281 ccgacggcaa gccggcgctg gacctggtgg ccgccgtggc cgcgaccttg cagcgagccg
     2341 gggtgagcga cgtcgacctg acgtcggccg cgtgcacggc ctgctgcccc gactacttct
     2401 cccaccgggc gcggggggag gcccaacgcc aggccttggt cgtctggcgc cgcccacccg
     2461 gcagccatgg gtgatccccg ggccgccctg actgccggcg cggtcgccga gcggctcgcc
     2521 gtggtccgga cccggatcgc ggccgcaggt ggcgatcccg ggcgggtccg ggtcgtggcg
     2581 gtgaccaagg gcttcgggcc ggcagcggtc gaggccgctg ccggcgccgg cctgttcgac
     2641 atcggcgaga actacgccca ggagctcggt ggcaaggtcg tcgcgaccgg ccacctggcg
     2701 gccctgcggt ggcacttcct cgggcccctc cagcgcaaca aggtggcccg attggcacag
     2761 gtggtggccg tgtggcagtc ggtcgaccgc cgggccgccg tcgacgccat cgccgcggcc
     2821 tcgcccggcg cctcggtctt catccaggtc aacctggccg aggatcccaa tcgccccggc
     2881 tgctcatggg ccgcggtggg cgatctcgtc cgccggggcc agggagcagg tctcgacgtc
     2941 cagggtctga tgggggtggc cccgctcggt catggctcgg gc
//
LOCUS       DASWGG010000102         4606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_745439, whole genome shotgun sequence.
ACCESSION   DASWGG010000102 DASWGG010000000
VERSION     DASWGG010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745439"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VF954_01885"
     CDS             <1..906
                     /locus_tag="VF954_01885"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX9529860.1"
                     /translation="GFFVTPTPVYSGKVRDIYDAGEDHLLFVASDRMSAFDVVMAEPI
                     PDKGRVLTAMTAFWFEELAGVAPSHLVSVDPADFPPEAGELADAAGRTMVVRRAEMLP
                     VECIVRGYLAGSAWKEYRASGTMHGQPLPAGLRESEQLPEPVFTPSTKAVTGHDENIG
                     FEQACELVGSDVAKAAREISMAAYQRGADRAASRGILIADTKFELGFIDGELAICDEV
                     LTPDSSRLWPASGWAPGTTPPSFDKQPLRDWLETTGWDKAPPPPLLPPDVVAATAERY
                     VAGYELITGRRFADWYGVDKARGRS"
     gene            903..1160
                     /gene="purS"
                     /locus_tag="VF954_01890"
     CDS             903..1160
                     /gene="purS"
                     /locus_tag="VF954_01890"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX9529861.1"
                     /translation="MTFDVLVEVSLRPGIADPQGATIERSLPALGFTGVADVRVGKAI
                     RFRVDGADEAAARTEVEDMCNRFLTNPVIEAATVTLVRGAP"
     gene            1231..1935
                     /gene="purQ"
                     /locus_tag="VF954_01895"
     CDS             1231..1935
                     /gene="purQ"
                     /locus_tag="VF954_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX9529862.1"
                     /translation="MSTVGVVVFPGSNCEWDVAEAISGLGGTPRMLWHADRGLSGVDA
                     VILPGGFAHGDYLRPGAIARFSPVMAAVADFARAGGPVVGICNGFQVLTEAGLLPGAL
                     QKNAGLKFLCQVAAVRVESTDSALTRGATLGSVLHIPINHFEGNYTCDQETLAELRAE
                     ERVVLRYLDNPNGSVDAIAAVCNQTRNVVGLMPHPERASHELLGSSDGAVLLASLLGA
                     AAGRSSARASEAPRPA"
     gene            complement(1932..2321)
                     /locus_tag="VF954_01900"
     CDS             complement(1932..2321)
                     /locus_tag="VF954_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529863.1"
                     /translation="MSSEHKEALALGREQGRAVRRYLEALEQHRPRRGRKRTADSIQA
                     RLDKIDAELEAADPLGRLHLTQERMDLELELATKSEAVDLTALEDDFVEAAKDYGRRK
                     GVSYAAWREAGVEAAVLKRAGVGRGAA"
     gene            2421..>4606
                     /gene="purL"
                     /locus_tag="VF954_01905"
     CDS             2421..>4606
                     /gene="purL"
                     /locus_tag="VF954_01905"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX9529864.1"
                     /translation="MSDTVHRALGLTDEEADAIARLLGREPNQLELAMYAVMWSEHCS
                     YKSSRIHLRRLPSEGQNVLVGPGENAGVIDAGDGIAVAFRIESHNHPSAVEPYQGAAT
                     GVGGILRDIFTMGARPIAVMDPLRFGSLDEPRTRWIFEGVVSGISGYGNAVGVPTVGG
                     EVVFDQTYADNPLVNVLCLGVMPTERLVLGQASGAGNLAVLLGSTTGRDGIGGASVLA
                     SAGFGDMADDAAKRPSVQVGDPFEEKRLIEACLALLDESLVVGIQDLGAAGLSGATSE
                     TASRGGVGMDVYLSEVPVREPGMAPFEIMTSESQERMLAIVRPEDLDRVLAICTRWEV
                     RASVVGTVTAGGRLRVLDRFDGEVLADVRAATLHDDAPRYDRPARAPEGRAARLADDP
                     AGLPDPPAGGCASDVLAMLVDPAWVYRQYDHMLFLNTVGAPGGDAAVLRLKAPGLPPS
                     RKGVALSTDGNARWCAVDPRRGTALVVAEAALNVACAGGRPVAAVNCLNFGNPEHSEV
                     MWQLTEAIDGMAEACRAFHIPVVGGNVSLYNESRGRDIDPTPVLGMLGLIDRLELPPP
                     PAALVEASVLLLLGPRGAALGGSRWAVERRGHRGGALPDLDYELHARLLGLVAGLVAD
                     RLVAGIHDVSDGGLGVCLAEMAVRGGVGFAVAGLAGHQELFSEAPSRVVVAVAPSQAG
                     EVEARAAAAGVPAATLGPAGGPRLVVAGLLDVSLLDVSLAEAHDAWR"
BASE COUNT          565 a         1640 c         1725 g          676 t
ORIGIN      
        1 ggtttcttcg tgacacctac gcccgtgtac tccggcaagg tccgtgacat ctatgacgcc
       61 ggcgaggacc acctgttgtt cgtcgcctcg gaccggatgt cggccttcga cgtggtgatg
      121 gccgagccca tccccgacaa ggggcgggtg ctgacggcca tgaccgcgtt ctggttcgag
      181 gagctggccg gggtggcccc gagccacctg gtcagcgtcg acccggcgga cttcccgccc
      241 gaggccgggg agctggccga cgccgcgggg cgcaccatgg tcgtgcggcg ggccgagatg
      301 ctgcccgtag agtgcatcgt gcggggctac ctggccggct cggcgtggaa ggagtaccgc
      361 gcctcgggga ccatgcacgg ccagccgctg ccggccgggc tgcgcgagtc ggagcagctg
      421 cccgagcccg tcttcacccc ctccaccaag gctgtcaccg gccacgacga gaacatcggc
      481 ttcgagcagg cctgcgagct ggtcggctcg gacgtggcca aggcggcccg cgagatctcc
      541 atggccgctt accagcgggg cgccgaccgg gccgcgtcgc gggggatact gatcgccgac
      601 accaagttcg agctcgggtt catcgacggc gagctggcca tctgcgacga ggtcctcacg
      661 ccggactcgt cgcggttgtg gcccgcgtcc gggtgggcgc cgggcaccac gccgccgtcg
      721 ttcgacaagc agccgctgcg ggactggctg gagaccacgg gctgggacaa ggcgccgccg
      781 ccgccgctgc tgccgccaga cgtggtggcg gccacggccg agcgctacgt cgccggctac
      841 gagctgatca cgggccggcg cttcgcggac tggtacgggg tcgacaaggc acggggtcgc
      901 tcgtgacctt cgacgtgctc gtcgaggtgt ccctccggcc cggcatcgcc gatccgcagg
      961 gcgccaccat cgaacggtcg ctgcccgcgc tgggcttcac cggtgtggcc gacgtgcggg
     1021 tgggcaaggc catccgtttc cgggtggacg gcgccgacga ggccgcggcg cgcaccgagg
     1081 tggaggacat gtgcaaccgc ttcctcacca acccggtgat cgaggcggcc accgtcaccc
     1141 tcgtgcgggg cgccccatga gcacggccgc cggcggggtt ggggccccgc cgtgcggagg
     1201 aggccggggt tggggccccg gccgggccaa atgagcacgg tcggcgtggt ggtgttcccg
     1261 gggtccaact gcgagtggga cgtggccgag gccatctccg gcctcggggg cacgccccgc
     1321 atgctgtggc acgccgaccg cggcctgtcc ggcgtcgacg ccgtgatcct gccgggcggc
     1381 ttcgcccacg gcgactactt gcgccccggg gccatcgccc gcttctctcc cgtcatggcc
     1441 gccgtggccg acttcgcccg ggcgggcggg ccggtcgtcg ggatctgcaa cggcttccag
     1501 gtcctgaccg aggccggcct cctgcccggc gccctgcaga agaacgccgg cctcaagttc
     1561 ctctgccagg tggccgccgt gcgcgtggag tcgaccgaca gcgccctcac ccggggcgcc
     1621 acgctcgggt cggtgctgca catccccatc aaccacttcg agggcaacta cacctgcgac
     1681 caggagaccc tggccgagct gcgcgccgag gagcgggtcg tgctgcgcta cctggacaac
     1741 cccaacgggt cggtcgacgc catcgcggcc gtgtgcaacc agacccgcaa cgtggtcggg
     1801 ctcatgccgc accccgagcg ggccagccac gagctgctcg gctcgtccga cggggccgtc
     1861 ctgctggcgt ccctgctggg cgcggcggcg ggcaggtcgt cggctcgggc ctcggaggcg
     1921 ccccggccgg cttaggcggc gccccggccc accccggccc gcttgagcac ggcggcctcc
     1981 accccggcct cccgccacgc cgcgtagctg acccccttgc ggcggccgta gtccttggcg
     2041 gcctcgacga agtcgtcctc cagggctgtg aggtccacgg cctcggactt ggtggccaat
     2101 tccagctcca ggtccatgcg ctcttgggtc aaatgcaggc gacccagcgg atcggcggcc
     2161 tccagctcgg cgtcgatctt gtccagcctg gcctggatgg aatccgcggt ccgcttccgg
     2221 ccccggcggg gacgatgttg ctccagtgct tcgaggtagc ggcgcacggc acgcccctgt
     2281 tcccgcccga gtgccagggc ctctttgtgc tctgacgaca tcggcggctt tgcgcgcttg
     2341 gctttgggag gcataccgac aatatagggg gaaactcggt cgcgtgcagg gatcaattgg
     2401 tcgggtgtta tcgtgcgccc gtgtctgaca ccgttcaccg ggctctgggc ctgaccgacg
     2461 aagaggccga tgccatcgcc cggctgctgg gtcgggagcc caaccagctc gagctcgcca
     2521 tgtacgcggt gatgtggagc gagcactgct cgtacaaatc ttcccgaata catctccgcc
     2581 ggctcccgag tgaggggcaa aatgtgctgg tcggaccggg cgagaatgcc ggggtcatcg
     2641 acgccgggga cggcatcgcg gtcgccttcc gcatcgagag ccataaccac ccgtcggccg
     2701 tcgagcccta ccagggcgcg gccacgggcg tgggcggcat tctgcgcgac atcttcacca
     2761 tgggcgcccg gccgatcgcg gtaatggacc ccctccggtt cggttcgttg gacgagcccc
     2821 ggacccggtg gattttcgag ggtgtggtga gcggcatctc cggctatggg aacgcggtgg
     2881 gtgtcccgac ggtgggcggg gaggtcgtgt tcgaccagac gtatgccgac aatccgctgg
     2941 tcaacgtgct gtgcctcggg gtcatgccga cggagcggct ggtgctgggc caggcctcgg
     3001 gcgccggcaa cctggcggtg ctgctcggct ccaccaccgg ccgcgacggc atcggcgggg
     3061 cgagcgtgct ggcctcggcc ggcttcgggg acatggcgga cgatgcggcc aagcgcccga
     3121 gcgtgcaggt gggcgatccc ttcgaggaga agcgcctgat cgaggcctgc ctggcgctgc
     3181 tggacgagtc cctggtggtg ggcatccagg acctgggggc cgcgggcctg agcggtgcca
     3241 cgagcgagac cgcctctagg ggcggggtgg gcatggacgt gtacctgtcg gaggtgccgg
     3301 tccgcgagcc cgggatggcg cccttcgaga tcatgaccag cgagagccag gagcggatgc
     3361 tggccatcgt ccggcccgag gacctcgacc gcgtgctcgc gatctgcacg cggtgggagg
     3421 tccgggcctc ggtcgtcggg acggtgacgg ccggcgggag gctgcgcgtc ctcgaccgct
     3481 tcgacggcga ggtgctggcc gacgtccggg cggcgaccct gcacgacgac gccccccgct
     3541 acgaccgccc cgcccgggcg cccgagggcc gggcggcgcg gttggcggac gacccggccg
     3601 gcctgcccga cccgccggcg ggcgggtgcg cctccgacgt gctggccatg ctcgtcgacc
     3661 cggcgtgggt ctaccgccag tacgaccaca tgctcttcct gaacaccgtc ggggcgccgg
     3721 gcggcgacgc cgctgtcctg cgcctcaagg cgcccggcct gcctcccagc cgaaagggcg
     3781 tggccctgtc gaccgacggc aacgcccgct ggtgcgcggt cgacccccgg cgcgggacgg
     3841 cgctggtggt ggccgaggcc gccctcaacg tggcctgcgc cgggggtcgg ccggtggccg
     3901 ccgtcaactg cctcaacttc ggcaaccccg agcactccga ggtcatgtgg cagctcaccg
     3961 aggccatcga cgggatggcc gaggcctgcc gcgccttcca catcccggtg gtggggggca
     4021 acgtgagcct ctacaacgag agccggggcc gcgacatcga ccccaccccc gtgctcggga
     4081 tgctagggct catcgaccgg ttggagctcc cgcccccgcc ggccgcgctc gtcgaggctt
     4141 cggtgctgct ccttctcggc ccgcggggcg cggcgctggg cggctcccgc tgggcggtgg
     4201 agcgccgggg ccatcgcggc ggggcgctcc ctgacctgga ctacgaactg cacgccaggc
     4261 tgctcgggct ggtcgccggt ctggtcgccg accgcctggt ggcagggatc cacgacgtct
     4321 cggacggcgg cctgggcgtg tgcctggccg agatggccgt gcgcggcggg gtcggattcg
     4381 cggtggcggg gctcgccggc caccaggagc tgttctccga ggccccctcc cgggtggtgg
     4441 tggcggtggc gccgtcgcag gccggcgagg tggaggcccg ggccgcggcc gcgggcgtgc
     4501 cggccgccac cctcgggccc gccggcgggc cgcgcctggt ggtggccggc ctgctcgacg
     4561 tcagcttgct cgacgtcagc ctggcagagg cccacgacgc ctggcg
//
LOCUS       DASWGG010000103         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_370754, whole genome shotgun sequence.
ACCESSION   DASWGG010000103 DASWGG010000000
VERSION     DASWGG010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370754"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VF954_01910"
     CDS             <1..522
                     /locus_tag="VF954_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529865.1"
                     /translation="GMEAMMEVLGQRLGYSAAWSALQGWKGDDGVVYRSSGHVCVAIG
                     TALDSTAAADRLESAARAWAATLPGAAVTRRGLTVEIRSCDPGTSFRGPAPAAPAPFD
                     VLSARSELLDQVVQVGDLPVPEGVCRVDSIIAQLGPAETTTLMAGQDPAAMNRTLAPA
                     GRVAATICHANRP"
     gene            666..1265
                     /locus_tag="VF954_01915"
     CDS             666..1265
                     /locus_tag="VF954_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9529866.1"
                     /translation="MEQPAENTAALDITGRRLMEAAGRILASDGPAALTVRRVSSEAG
                     CSTMCLYDRFGDKSGVVDRLFIDGFASLEAATVAALAGDDPLGCIRASCGAFRTWALD
                     NRTHYAVMFARAVPDYTPSPAAIDNAKRALRTMERGIQAAIDAGSLAARPACDLAHVI
                     WAALHGLVSLELAGMLSAEAAGRYHQQVVDALLRGLAPE"
     gene            complement(1281..1682)
                     /locus_tag="VF954_01920"
     CDS             complement(1281..1682)
                     /locus_tag="VF954_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9529867.1"
                     /translation="MARPMIGVPFVVLGAEAVKDPGPRAERVTTVGPKVARALHLPED
                     PVSLVRINGAAQVGAGALLAIGRLPRLAALVLAGSLVPTTLTGHPFWEETDKQRRAHQ
                     QVQFLKNVGLLGGLLLAVVDPGRRRRAPRPG"
     gene            complement(1801..>2092)
                     /locus_tag="VF954_01925"
     CDS             complement(1801..>2092)
                     /locus_tag="VF954_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529868.1"
                     /translation="SVPVAWAGDDLVAETGRRTARNAVARSAVSVLWAPLEPGGYSLI
                     VDGEAGVSTLDDPGREGGRIAVRPSAAVLHRSAAGPDGLGACGSDCEPVMRP"
BASE COUNT          289 a          793 c          752 g          258 t
ORIGIN      
        1 gggatggagg ccatgatgga ggtgctcggc cagcggctcg gctacagcgc ggcgtggtcg
       61 gcgcttcagg gctggaaggg cgacgacggc gtcgtgtacc ggtcgtccgg ccacgtttgc
      121 gtcgccatcg gcaccgccct cgactccacg gcggcagccg accggctcga gagcgcggcc
      181 cgggcctggg cggccacctt gcccggcgcc gcggtcacac gccgcggcct caccgtcgag
      241 atccgttcgt gcgatcctgg gaccagcttc cgcggccccg cgccggcggc accggccccc
      301 ttcgacgtcc tgtcggcccg ctccgagctg ctcgatcagg tcgtccaggt gggcgacctc
      361 ccggtgcccg aaggggtttg ccgggtggac tccatcatcg cccagctcgg gccggccgag
      421 accacgacgc tcatggcggg gcaggacccg gcggcgatga accggaccct cgctccggcc
      481 gggcgcgtgg cggcgacgat ctgccatgcc aaccgtccct agcgacggac cccctggcag
      541 cgcctctctc cgacgtcgcg gcggctaatg tcaccggcca gcggatcggc cggtgggacg
      601 gccggtcaca caggcggtag agaacgcagg cggtagagaa acgtagagaa acacaggtgg
      661 agccaatgga gcagccagcg gagaacacgg ccgcgctcga catcaccggc aggcgcctaa
      721 tggaggccgc cgggcggatc ctggcgtcgg acgggcccgc cgccctcacc gtccgacggg
      781 tctcatccga ggccggttgc tccaccatgt gcctgtacga ccgcttcggc gacaagtcgg
      841 gggtcgtcga ccgcctcttc atcgacggct tcgccagtct cgaagcggcc accgtcgccg
      901 ccctcgccgg cgacgacccc ctgggctgca tccgcgccag ctgcggagcg ttccggacct
      961 gggccctcga caaccggact cattacgccg tcatgttcgc cagggccgtc cccgactaca
     1021 cgccgtcgcc ggccgccatc gacaatgcca agcgagcatt gcggaccatg gagcgcggca
     1081 tccaggcggc catcgacgcc ggctcgctgg ccgcccggcc ggcgtgcgac ctggcccacg
     1141 tcatctgggc cgcgctccac ggcctggtga gcctggagct ggcgggcatg ctgtcggcag
     1201 aggccgccgg gcggtaccac cagcaggtcg tcgacgccct gctccggggg ctggccccgg
     1261 agtagccctg cgttcggccc tcagcccggc cggggcgccc gccggcggcg gccggggtcg
     1321 accacggcaa ggagcaggcc cccgagcaga ccaacgttct tgaggaactg gacctgctga
     1381 tgggcccggc gctgcttgtc ggtctcctcc cagaaggggt ggccggtcaa tgtcgtcggc
     1441 acgagcgagc cggccagcac cagggccgcc agccgcggta ggcggccgat cgcgaggagc
     1501 gcaccggcgc ccacctgggc ggccccattg atccgaacga gcgagaccgg gtcttcgggc
     1561 aggtgtaggg cgcgggccac cttgggtccg acggtggtga cgcgctcggc tcggggtccg
     1621 gggtccttca ccgcctcggc gccgaggacc acgaacggca caccgatcat gggtcgggcc
     1681 acgcgccgta gaaggttcac tccgagaccg taccccgtcg ggccgcgccc gacaccacgg
     1741 cgtgccggcc gacccccggg cgccagcgcc ggccagggcg cgcccaacgc ctgaaccggg
     1801 tcaggggcgc atcacgggct cgcagtccga gccgcacgct cccagcccgt cgggcccggc
     1861 cgccgaccgg tgcagcaccg cggccgaggg ccgcacggcg atgcgcccgc cctcgcggcc
     1921 ggggtcgtca agggtcgaga ccccggcctc gccgtccacg atcaggctgt agccgcccgg
     1981 ttccaacggt gcccacagca cgctgactgc ggaccgggcc acggcgttgc gggcggtgcg
     2041 gcgaccggtc tcggccacca ggtcgtcacc cgcccacgcc acggggactg ac
//
LOCUS       DASWGG010000104         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3002329, whole genome shotgun sequence.
ACCESSION   DASWGG010000104 DASWGG010000000
VERSION     DASWGG010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3002329"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VF954_01930"
     CDS             <1..489
                     /locus_tag="VF954_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529869.1"
                     /translation="LAAFSGVLADVGIEVAQAVLATWDDGAALQAFVVRAVPAPDGRE
                     LQARFEASLGRPLCSPPVGDAEVSFDGAPSPLYTPCRVRAADRPGLLHSIAVAIAVAG
                     GDVHAARVVTAGGMARDDFDVSDRDGHKLVAADRAAIRAAIIEGVGARRSGRRPAVGP
                     RR"
     gene            complement(497..847)
                     /locus_tag="VF954_01935"
     CDS             complement(497..847)
                     /locus_tag="VF954_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529870.1"
                     /translation="MDATPRFLQAISSFRGHGLDEYLPLDPPLTYTVPDEAQAQCVYF
                     RGGNSADQLICVVLVRDGRPMRLFPIGARAAIHLPLRVTEDLMSDTHLEVQVAAPEGV
                     AGTVVVDLGLLVFS"
     gene            957..>1605
                     /locus_tag="VF954_01940"
     CDS             957..>1605
                     /locus_tag="VF954_01940"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX9529871.1"
                     /translation="MLEPLAGWTVGVTADRRAAEQVAMLERRGASVRCGPVIRTFPLD
                     AEAGLRATTADLVSRPPDLLVATTAIGIRSWFASAWTWGAGERLPGCLGEARILARGP
                     KAAAAVTGEGLGVAWKAPGETMRSVLDHLLAQPLAGRRVAVQLPGRPQPWFTAALRAA
                     GAEVVEVAVYSATLPADGGPAHRLIDALLAGELDAVTLTTPQALTNLLDIAGPRRE"
BASE COUNT          201 a          570 c          615 g          219 t
ORIGIN      
        1 ctggccgcct tcagcggcgt cctcgccgac gtcggcatcg aggtggccca ggccgtgctg
       61 gccacctggg acgacggtgc tgccctccag gccttcgtcg tgcgggcggt gccggcaccc
      121 gacgggcgcg agcttcaggc ccggttcgag gcctcgctcg gccggccgct gtgttcgcct
      181 cccgtcgggg acgccgaggt cagcttcgac ggcgcgccgt cgccgttgta cacgccgtgc
      241 cgggtacggg ccgccgaccg gcccgggttg ctgcactcga tcgctgtcgc catcgccgtg
      301 gccggcggcg acgtgcacgc agcccgggtg gtaacggcgg gcggcatggc gcgggacgac
      361 ttcgatgtca gcgaccgaga cggccacaag ctcgtcgccg cggaccgcgc tgccatccgc
      421 gccgccatca tcgagggcgt cggcgcgcgg cggtccggtc gtcgccccgc cgtcggaccg
      481 cgaaggtgac cctttctcag gagaagacca gcagccccag gtccacgacc acggtgccgg
      541 ccaccccttc cggggcggcg acctggacct cgaggtgcgt atcggacatg aggtcctcgg
      601 tcacccgcag gggaaggtga atggcggcgc gggcgccgat ggggaacagc cgcatcgggc
      661 gcccgtcgcg caccagcacc acgcagatca gctggtccgc gctgttgccg ccccggaagt
      721 acacgcactg ggcctgggcc tcgtcgggca ccgtgtaggt gagcggcgga tcgagcggca
      781 ggtactcgtc aaggccgtgg ccccggaagg aggagatggc ctggaggaac ctgggcgtgg
      841 cgtccacctc tccacagtgg cacgagtcgg cgagtcaggc ttcggtcagg aagttgtcgc
      901 acgatcctgc cggtagcgga aaccggccgg aaacagcccg ccgagacgat cgaggtgtgc
      961 tggagcctct ggccgggtgg acggtcggcg tcaccgcgga ccggcgcgcc gccgagcagg
     1021 tggccatgct ggagcggcgg ggtgcctctg ttcggtgcgg cccggtcatc cggacatttc
     1081 cgctcgacgc ggaggcgggc ttgcgcgcca ccacggcgga cctggtgtcc cgcccacccg
     1141 acctgctcgt ggcgacgacg gcaatcggca tacggagctg gttcgcgtcg gcctggacgt
     1201 ggggcgcggg cgagcgactg cccggctgcc tgggcgaggc ccggatcctg gcccgcggcc
     1261 ccaaggctgc ggccgcggtg accggcgagg ggctcggcgt ggcgtggaag gcgcctggcg
     1321 agacgatgcg aagcgtgctc gaccacctgc tggcccaacc cctcgcgggg cgccgggtgg
     1381 cggtccagct gcccggtcgg ccgcagccgt ggttcacggc cgcgttgcgg gcggctgggg
     1441 ccgaggtggt cgaggtggcg gtgtactccg cgaccctgcc ggccgacggc ggtcccgctc
     1501 accgcctcat cgatgccctg ctggccggcg agctcgacgc cgtgacgctc acgacgcctc
     1561 aggcccttac caacctgctc gacatcgccg ggccgcgccg ggagg
//
LOCUS       DASWGG010000105         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_746303, whole genome shotgun sequence.
ACCESSION   DASWGG010000105 DASWGG010000000
VERSION     DASWGG010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746303"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VF954_01945"
     CDS             complement(<1..1004)
                     /locus_tag="VF954_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX9529872.1"
                     /translation="MAGPARRHVLLVGMMGAGKTTVGGLLAERLGRPYCDSDLEVEAA
                     TGRSVAEIFADEGEAAFRAQESAALALAVAEPGARVISVAGGAVLAAANRALIEAAGL
                     VVWLRARVGTLAVRVGAGEGRPLLGGDPAAALARLDTARRDLYAEVADLTIDVDDLTA
                     GEVVDAVVAAMDAADAPAAAAARTADGARTGAGGLITVTVDLGERSYPVLVGAGARHR
                     LLEVLPTGARRAAVITQAAVGVPVDPGVEHRSFLIGDGEESKSLETVEDLCRRLARWG
                     LTRGDVIVAVGGGVVTDTAGFVAAVYHRGVAVIHVATTLLAQVDAAIGGKTGVNLPEG
                     "
     gene            complement(1030..>1722)
                     /locus_tag="VF954_01950"
     CDS             complement(1030..>1722)
                     /locus_tag="VF954_01950"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX9529873.1"
                     /translation="VVSKSATRPTPEDLERIDASPLRCFDPDAEAAMLEEVKATAKAG
                     DSLGGVVEVLAYGVPVGLGSHAQWDRRLDALLGQALMSIQAVKGVEIGDGFEMAARRG
                     SEAHDAIEWDAAGRGYLRRTDRAGGVEGGMSNGSLLVARAAMKPLATLNRPTLKTVDV
                     VTKQESVSFKERTDVTAVPAMGVVAETMVALVLAGEALRKFGGDSIAELARNHRAFCA
                     DLAEGDVGGTIG"
BASE COUNT          213 a          679 c          618 g          212 t
ORIGIN      
        1 ttgccctcgg ggaggttgac ccccgtcttg ccgccgatgg ccgcgtcgac ctgggcgagg
       61 agggtggtgg ccacgtggat gacggccacg ccccggtggt acacggcggc cacgaagccc
      121 gccgtgtcgg tgaccacccc gccgccgacg gcgacgatca cgtcgccgcg ggtcaggccc
      181 caccgggcga gacggcggca caggtcctcc acggtctcga gcgacttgga ctcctcgccg
      241 tcgccgatga ggaagctgcg gtgctctacg cccgggtcga cgggcacgcc gaccgccgcc
      301 tgggtgatca ccgcggcgcg gcgcgcgccg gtcggcagga cctcgaggag gcggtgccgg
      361 gcgcccgccc cgacgagcac cgggtaggac cgctcgccga ggtcgaccgt gacggtgatc
      421 agcccgcctg ctccggtccg ggcgccatcc gcggtccggg cggccgcggc ggccggggcg
      481 tccgccgcat ccatggcggc cacgaccgcg tcgaccacct cgccggccgt gaggtcgtcc
      541 acgtcgatgg tcaggtcggc cacttcggcg tacaggtccc ggcgggcggt atcgaggcgg
      601 gccagggctg cggccgggtc gccgcccagc agcggccggc cctcgccggc gcccacccgg
      661 accgccagcg tgcccacccg ggcccggagc cacacgacca ggccggcggc ctcgatgagc
      721 gcccggttgg ccgcggcgag cacggcgccg ccggcgacgg agatgaccct ggctccgggc
      781 tcggccaccg caagggccag agccgcgctc tcctgggccc ggaaggcggc ctcgccctcg
      841 tcggcgaaga tctcggcgac cgagcgccct gtcgcggcct cgacctccag gtcgctgtcg
      901 cagtacggac gccccagccg ctcggccagc aagccgccca cggtggtctt gcccgcgccc
      961 atcatgccca ccagcagcac gtgacgtcgg gccgggccag ccaccttgtc tctctcggcc
     1021 aactcgccct cagccaatcg tgccgccgac gtcgccctcg gccaggtcgg cgcagaaggc
     1081 ccggtggttg cgcgccagct cggcgatcga gtcccccccg aacttgcgca gcgcctcccc
     1141 ggccagcacc agggccacca tggtctcggc cactaccccc atggcgggaa cggccgtgac
     1201 gtcggtgcgc tccttgaacg aaaccgactc ctgcttggtc accacgtcca ccgtcttcag
     1261 ggtgggccgg ttgagggtgg cgagcggctt catggccgcc cgggcgacga gcagagagcc
     1321 gttggacatg cccccctcga ccccgccggc ccggtcggtg cggcgcaggt agccgcgccc
     1381 ggcggcatcc cactcgatgg cgtcgtgggc ctccgagccc cggcgggcgg ccatctcgaa
     1441 gccgtccccg atctcgaccc ccttgacggc ctggatgctc atgagcgcct ggccgaggag
     1501 ggcgtccagg cgccggtccc actgggcgtg gctgcccagc cccacgggca cgccgtaggc
     1561 cagcacctcg accaccccgc ccagcgagtc gcccgccttg gccgtggcct tgacctcctc
     1621 gagcatggcg gcctcggcgt cggggtcgaa gcagcgcagc ggcgaggcgt cgatgcgctc
     1681 gaggtcctcg ggcgtcggcc gggtcgcgga cttggacacg ac
//
LOCUS       DASWGG010000106         2214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2684689, whole genome shotgun sequence.
ACCESSION   DASWGG010000106 DASWGG010000000
VERSION     DASWGG010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2684689"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /gene="hflX"
                     /locus_tag="VF954_01955"
     CDS             <1..1237
                     /gene="hflX"
                     /locus_tag="VF954_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX9529874.1"
                     /translation="VTPEQVEADLDELALLVDTAGADVAARVVQRRDRPDPASFVGKG
                     KAEEIHELAEAVDADTVVFDEDLTPAQQRNLEKIFGRTAIDRTAVILDIFAQNARSQE
                     GKAQVELALLRYRLPRLRGKGRSLSQQAGAMGAGAARAPIGSRGPGETQLEVDRRRLV
                     RRMSRLEQDLEQLSRTRHTQRRGRSRSGLHMVSLVGYTNAGKSTLLNALTQAGVLVED
                     RLFATLDPRVRRLELPGGEAVLLSDTVGFVRKLPHQLVEAFRSTLEVVGESDLLVHVV
                     DSSAPDPEAQIEAVRAVLAEIDADGVPELLAFNKRDVAPDAAGRLARNHPGSVTLSSR
                     TGGGIPELLGATAERLRSLARIVELVVPFDRGDVVAAVHREGEVMSERYEEGGTHLVA
                     RLDAAGAARFRSFARAAGA"
     gene            1258..>2214
                     /locus_tag="VF954_01960"
     CDS             1258..>2214
                     /locus_tag="VF954_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9529875.1"
                     /translation="MPPAYPYDRLSGARAKASAHDGGVVDLSVGTPCDAPPPAVVEAL
                     GRSDAERGYPPSAGSPALREAACEWMRRRLGVRVEPAHVGACIGTKELVATLPQWLHL
                     RHPGRDTVLYPAVAYPTYEMGAVLAGCRAVPVPVDAGFHPVLDAIATDDAARALLLWV
                     NSPGNPCGQVDDLGLAAAWGREHGVPVFSDECYVEFTWSGPRRTILEHGHDGLVAVHS
                     LSKRSNLAGARVGFYAGDPELVHYLSEVRKHAGMMVPGPVQAAAVVALSDDDHVEAQR
                     DRYRARLERMRAVLATVDVHAPQPAGSFYLWAPAPDGDGWGLT"
BASE COUNT          263 a          831 c          837 g          283 t
ORIGIN      
        1 cgtcacgccc gagcaggtcg aagccgacct cgacgagctg gctctgctcg tcgacaccgc
       61 cggcgcggac gtggccgccc gggtggtgca gcggcgggac cggcccgacc cggcgagctt
      121 cgtgggcaag ggcaaggcgg aggagatcca cgagctggcc gaggccgtgg acgcggacac
      181 ggtggtgttc gacgaggacc tgacgccggc ccagcagcgc aacctggaga agatcttcgg
      241 gcgcaccgcc atcgaccgca cggccgtgat cctggacatc ttcgcccaga acgcccggtc
      301 ccaggagggc aaggcccagg tcgagctggc cctgctgcgc taccggctgc cccgcctgcg
      361 cgggaagggc cggtcgctgt cccagcaggc cggcgccatg ggcgccggcg cggcccgggc
      421 gccgatcggc tcccgggggc cgggcgagac ccagctggag gtcgaccgcc gccgcctggt
      481 ccggcgcatg agccgcctcg agcaggacct cgagcagctc tccaggaccc gccacaccca
      541 gcgccggggc cggagccggt cgggcctgca catggtctcg ctcgtgggct acaccaacgc
      601 aggcaagtcg acgctgctca acgccctcac ccaggcgggc gtgctggtgg aggaccgcct
      661 cttcgccacg ctcgacccgc gcgtgcgccg gctggagctt cccggcggcg aggcggtgct
      721 gctctcggac acggtcggct tcgtccggaa gctgccccac cagctggtgg aggccttccg
      781 ctcgacgctc gaggtggtgg gggagtccga tctgctggtc cacgtggtgg acagctcggc
      841 gcccgacccc gaggcccaga tcgaggcggt gcgcgccgtg ctggccgaga tcgacgccga
      901 cggggtcccc gagctgctgg ccttcaacaa gcgcgacgtg gcgcccgacg ccgccgggcg
      961 gctggcccgc aaccatcccg ggtcggtgac gctctcgtcc cggacgggcg gcggcatccc
     1021 cgagctgctg ggcgctaccg cggagcggct gcgatcgttg gcccgcatcg tggagctggt
     1081 ggtgcccttc gaccggggcg acgtggtggc ggccgtgcac cgtgagggcg aggtcatgag
     1141 cgagcgctac gaggagggcg ggacccacct ggtggcccgc ctcgacgccg ccggggcggc
     1201 gcggttccgc tcgttcgccc gggcggccgg ggcgtgaagg ggcgggcgcc gggtttcgtg
     1261 ccgccggcgt acccctacga ccggctctcc ggggccaggg cgaaggcgtc cgcccacgac
     1321 ggcggcgtcg tcgacctgtc cgtcggcacg ccctgcgacg ccccgccgcc ggcggtcgtc
     1381 gaagccctcg gccgctccga cgccgagcgg ggctatcccc cttcggcggg ctcgccggcg
     1441 ctgcgggagg cggcgtgcga gtggatgcgg cgccgcctcg gcgtccgggt cgagccggcc
     1501 cacgtgggcg cctgcatcgg caccaaggag ctcgtggcca ccctcccgca gtggctgcac
     1561 ctgcgccacc cggggcgcga caccgtgctg tacccggccg tggcctaccc gacctacgag
     1621 atgggcgccg tgctggccgg ctgccgggcg gtcccggtcc cggtcgacgc cggcttccac
     1681 cccgtgctcg atgccatcgc caccgacgac gccgcccggg ccctgctgct gtgggtgaac
     1741 tcgccgggca acccctgcgg ccaggtcgac gacctcggcc tggccgcggc gtggggccgg
     1801 gagcacggcg tgccggtgtt cagcgacgag tgctatgtcg agttcacctg gtccgggccc
     1861 cgccggacca tcctcgagca cggccacgac ggcctggtcg cggtgcactc gctgtccaag
     1921 cgatccaacc tggccggggc ccgggtcggc ttctacgcgg gtgaccccga gctggtccac
     1981 tacctgtcgg aggtgcgcaa gcacgcaggg atgatggtgc ccggcccggt ccaggccgcg
     2041 gccgtcgtcg ccctgtccga cgacgatcac gtcgaggccc agcgcgatcg gtaccgggcc
     2101 cggctcgagc gcatgcgcgc cgtgctggcc actgtcgatg tccacgcgcc gcagccggcc
     2161 ggcagcttct acctctgggc gccggccccc gacggcgacg gctgggggct gacc
//
LOCUS       DASWGG010000107         3968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_51729, whole genome shotgun sequence.
ACCESSION   DASWGG010000107 DASWGG010000000
VERSION     DASWGG010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51729"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VF954_01965"
     CDS             <1..1125
                     /locus_tag="VF954_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529876.1"
                     /translation="QTTTSSDGNTTTTGADGSSTTTAPDGNINTTSPDGSQTTTAPDG
                     NTTTTGSDGSQTTTTSDGNNTTTGADGSTTTTGADGTQTTTSADGATTTTTATDGSQT
                     TQGADGSTTTTTADGSKTTTSADGTVTATAADGTTSITNPDGGSTVMQPDGTITVTGA
                     DGSSSTSNPDGSTSTAAADGSSTTTAADGTKTTTTADGTVTTTNPDGTQTTNGPDGSV
                     TVTSADGSQSTIGADGTETVTNPDGSSTVRGADGTVTNYDANSQPVPEPGQTPPAPPA
                     RNPSTVHQSHQPHASHQQPAQPAAHHDHTPHHQPAPHNTHNTHNTHAPHNTHNAHLQL
                     AQHLPHNQHKAPKTQPSPHHPKGGERHPLHEAHRTQMAAS"
     gene            1164..1754
                     /locus_tag="VF954_01970"
     CDS             1164..1754
                     /locus_tag="VF954_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529877.1"
                     /translation="MHSSPEGGRAPALVLAAAMALAGTGILTVAAKLGDRGRPRPRAR
                     ATSTALAPATTAPTAPTTTAAPSALTIIGVGPGVNQDPFYPAAAVSQVSCGTAPAGRF
                     VRLEVPAGGTSSRSALAATTTMTVVPGKAVLRDHTGKVLYEEHLASIATGRAGALVLS
                     LVSTTFVGGDGRAVSQGAVNVSGDYTCPDTDVAFGT"
     gene            1786..3825
                     /locus_tag="VF954_01975"
     CDS             1786..3825
                     /locus_tag="VF954_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX9529878.1"
                     /translation="MTADRPTDRYQPALDGLRACAVAAVIAYHLGMRWMRGGYLGVDL
                     FFVLSGYLITGLLVDEWEKGGAIRLRAFWARRARRLFPALLVVLVAIGAWVAFGGPGI
                     DRYPLRADGLATLFYSANWHAIAAHRSYFARFAAPSPLEHTWSLAIEEQFYALWPLVV
                     IGLLAVSRRIRRFPLVATSALALASAAAMALLFHPGQDPSRVYFGTDTRAFELLVGAG
                     LAFALASRPRLSPSGQRALHATAIVAATAIGGLWATAGGPPAWMFRGGMVGAAGLVAV
                     MVWSVTEADRGWLGRLLSLRPLRWLGRVSYGLYLWHWPVVVIVSPATTTLRGPALDAA
                     RIGLTLALATISFYLVELPIRIGKLAGWPARVLAPTATGATAAVLLMATLPTAAAVAT
                     VTAAPPASTAVAPAASPLPPGTDPGPSPIKLPAGRVISPSDPLRVMLIGDSVMYDDEP
                     AITAALQATGMAQTTELAFPGWGLTRGSGWRTDWPSNLARFRPDIVMGTWSWDDSAAR
                     ADLAGYAELLDEGITVLTSGPHAAAGVVLLEFPRGGPRLGQTAAQAASEESGRGAWNL
                     VASQRAAAHPGQVSFLPVAASLDIDGRFSTWLQNGDGSWARARKIDALHLCPTGAARL
                     ATAVAGQLAVAVGFPAPAPSWWSGRWSRDPRYDNPPGSCPDDQPPAGFAQQLTSA"
BASE COUNT          583 a         1614 c         1310 g          461 t
ORIGIN      
        1 cagacgacca ccagctccga cggcaacacg accaccaccg gggccgacgg cagctcgacc
       61 acgacggcgc ccgacggcaa catcaacacc acctcgcccg acggcagcca gaccaccacg
      121 gcgccggacg gcaacaccac gaccaccggc tccgacggct cgcagacgac caccacctcc
      181 gacggcaaca acaccaccac aggggcggac ggcagcacca cgaccaccgg cgccgacggc
      241 acccagacca ccaccagcgc cgacggggcc acgacgacca ccaccgcgac cgacggctcg
      301 cagacgaccc agggcgccga cggctccacc accacaacca cggccgacgg gagcaagacg
      361 accacgtcgg ccgacggcac ggtgaccgcc acagccgccg acggcaccac gtcgatcacc
      421 aacccggacg gcggctcgac ggtgatgcag ccagacggca cgatcaccgt cacgggggcc
      481 gacggctcct cgtcgaccag caacccggac ggatccacgt ccaccgccgc cgccgacggt
      541 tccagcacca cgaccgccgc cgacgggacg aagaccacca cgacggccga cggcacggtc
      601 acgaccacca atccggacgg cacgcagacc accaacggcc ccgacggctc ggtcaccgtc
      661 acttcggccg acggcagcca atcgacgatc ggggccgacg gcaccgagac ggtcaccaac
      721 cccgacgggt cgagcacggt caggggtgcc gacgggaccg tcaccaacta cgacgccaac
      781 agccagcccg ttcccgaacc gggacagacc ccgccggccc cgccggcgcg gaacccgtca
      841 acggtccacc agagccacca gccgcacgcc tcccaccaac agccggctca gccggcggcg
      901 caccacgacc acaccccgca ccaccagccg gcgccgcaca acacccacaa cacccacaac
      961 acgcatgcgc cgcacaacac ccacaacgcc catctccagc tggcccagca cttgccccac
     1021 aatcagcaca aggcacccaa gacccaaccc agcccgcacc atcccaaggg cggagagcgc
     1081 cacccgctcc atgaagccca ccggacgcag atggcggcca gctagccgct gaccgcctcg
     1141 ggcttgtcag acatacttgg ctcgtgcaca gctctccgga agggggccgc gccccggcgc
     1201 tagtcctggc cgcggccatg gcgctcgccg ggaccggcat cctcacggtc gcggcgaagc
     1261 tgggcgaccg gggccggccc aggccccgag cccgcgccac ctcgacggcg ctcgcacccg
     1321 ccaccaccgc cccgacggca ccgacgacga ccgcggcgcc gagcgccctg accatcatcg
     1381 gagtgggccc gggcgtgaac caggacccct tctacccggc cgcggccgtg tcccaggtca
     1441 gctgcgggac ggctcccgcc gggcggttcg tgcgcttgga ggtgcccgcc ggcggcacct
     1501 cgtctcgatc ggcgctggcc gccacgacca cgatgaccgt cgtgccggga aaggcggtgc
     1561 tgcgcgacca cacgggaaag gtgctctacg aggagcatct ggcatccatc gccaccggtc
     1621 gcgccggggc gctggtgctg agcctggtga gcaccacgtt cgtcggaggc gacggccggg
     1681 ccgtgagcca gggggcggtc aacgtctccg gcgactacac gtgcccggac acggacgtgg
     1741 ccttcggcac ctgagcccgc cggtaccatc tcctggggct ccccgatgac cgcagaccga
     1801 cccaccgatc gctaccagcc cgccctcgac ggcctccggg cctgcgccgt ggccgcggtc
     1861 atcgcctatc acctggggat gcgctggatg cggggcgggt atctcggcgt ggacctgttc
     1921 ttcgttctgt cgggctacct catcaccggg ctgctggtcg acgaatggga gaaaggaggg
     1981 gcgatccggc tacgggcgtt ctgggcccgg cgggcccggc ggctgttccc ggccctgctc
     2041 gtcgtgctcg tcgccatcgg cgcgtgggtg gccttcggcg ggcccggcat cgaccgttac
     2101 ccgctgaggg cggacggcct ggccacgctc ttctactcgg ccaactggca cgccatcgcc
     2161 gcccaccgca gctacttcgc ccgatttgcg gccccctccc ctctcgagca cacctggtcg
     2221 ctggccatcg aggagcagtt ctacgccctg tggcccctgg tcgtgatcgg cctcctcgcc
     2281 gtgagccggc gcatccgccg gttcccgctc gtcgccacct cggcgctggc gctcgcgtcc
     2341 gcggcggcga tggccctgct cttccatccc ggccaggacc cgagccgcgt ctacttcggc
     2401 accgacaccc gggccttcga gctgctcgtc ggggctggcc tcgcattcgc cctcgccagc
     2461 cgcccgcggc tgagcccgtc cgggcagcgc gccctgcatg ccaccgccat cgtggcggcc
     2521 acggcgatcg gggggctgtg ggccacggcc ggagggccac cggcgtggat gttccggggc
     2581 ggcatggtgg gcgcggccgg gctggtcgcc gtcatggtgt ggagcgtgac cgaggccgac
     2641 cgcgggtggc tcggccgctt gctgtcgctc cgcccgctgc gctggctggg ccgggtgtcc
     2701 tacggcctgt acctgtggca ctggcccgtc gtcgtgatcg tgtcgcccgc caccaccacg
     2761 ctgcgcggcc ccgccctcga cgcggcccgc atagggctca ccctcgccct ggccacgatc
     2821 agcttctatc tggtcgagct gcccatccgg atcgggaagc tggccggttg gccggcgcgc
     2881 gtcctcgccc cgacggccac gggagcgacg gccgccgtcc tcttgatggc gacccttccc
     2941 acggcggccg cggtggcgac ggtcaccgcg gccccgccgg cgtccaccgc ggtcgcgccg
     3001 gccgcgtcgc cacttccacc cggcaccgac ccgggcccgt ccccgatcaa gctgccggcc
     3061 ggtcgggtca tttccccgtc cgatccgctg cgggtgatgc tcatcgggga ctccgtgatg
     3121 tacgacgacg agcccgccat caccgccgcg ctccaggcga ccgggatggc gcagaccacg
     3181 gagctggcct tccccggctg ggggctgacc cggggctcgg gctggcggac cgactggccc
     3241 agcaacctgg cgcgattccg ccccgacatc gtcatgggca cgtggagctg ggacgacagc
     3301 gccgcccggg ccgacctggc cggctacgcc gagctgttgg acgagggcat caccgtgctg
     3361 accagcgggc cccatgccgc ggcaggggtg gtgctcctcg agttcccgcg cggcgggccg
     3421 cgcctcgggc agacggccgc ccaggccgcc tccgaggaat cgggacgggg ggcgtggaac
     3481 ctggtcgcgt cgcagcgggc cgcagcccac cccggccagg tgagcttcct ccctgtggcc
     3541 gcctcgctcg acatcgacgg gcgcttctcg acatggctcc agaacggcga cgggtcctgg
     3601 gcgcgggccc gcaagatcga cgccctgcac ctgtgcccga cgggagccgc ccggctggcc
     3661 acggcggtgg cgggccagct ggccgtcgcg gtcgggttcc ccgcgccggc cccctcatgg
     3721 tggagcgggc ggtggtcccg tgatccccgt tacgacaacc cgcccggcag ctgccccgac
     3781 gaccagcccc cggccggctt cgcccaacag ctcacgagcg cctgaccccg gccaggccgg
     3841 gaagctcgaa ccacacctgc ttctcctgcg ccccccgctc gtagccccac ttcgaggcca
     3901 gcgcctcgat gaggtcgacg ccccggccgt gatcggcggc cggcggggcg tgctggggcc
     3961 ggggtgcg
//
LOCUS       DASWGG010000108         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_752694, whole genome shotgun sequence.
ACCESSION   DASWGG010000108 DASWGG010000000
VERSION     DASWGG010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752694"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VF954_01980"
     CDS             complement(<1..714)
                     /locus_tag="VF954_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9529879.1"
                     /translation="MIPTTEIPDLEEYRARARAWLSGNLARREGPPARRSLHHYDASQ
                     KPADRALQRKLYEAGYAGISWPAEYGGQGLPDAYEAAFNDEAEPYVTPNFGPLIITTY
                     GVNVPTILAHGQPAFLRQLIPEVLAGEALFCQLFSEPTGGSDLAGARTRATRDGDRWV
                     ISGQKTWSTFAHHADWGLCLARTNWDVPKHRGLTWFAIPTSLSGVDIRQIRQLDQTDG
                     FCEVFLDDVAVPDLYRIGDV"
     gene            complement(726..>1500)
                     /locus_tag="VF954_01985"
     CDS             complement(726..>1500)
                     /locus_tag="VF954_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9529880.1"
                     /translation="LAAGEQVASWVVADPSGDVAPATAVVARADGDGYSLSGRALVQD
                     GASVDWLLVTAGGGQGLSQFLLPRSAATGVAPRACHDVTLRFSTVTFDGVHVDATSLV
                     GPAGGADDDVEHQLRLATVLVAAETVGAMDALFEMARQYALDRTAFGRPIGSFQAVKH
                     QLADMSLSLEAARAVTANAVEVLDAGSPDAGEVASIAKAWTGDAGIELAQGCLQVFGG
                     IGYTWEHDSHLYLRRITMNSLLYGSPDWHRERICQVHGL"
BASE COUNT          205 a          509 c          573 g          213 t
ORIGIN      
        1 cacgtctccg atgcggtaca ggtccgggac ggccacgtcg tcgaggaaga cctcgcagaa
       61 gccgtcggtc tggtccagct ggcggatctg gcggatgtcg acgcccgaca gcgaggtggg
      121 gatggcgaac caggtgaggc cgcggtgctt ggggacgtcc cagttggtcc gggccaggca
      181 caggccccag tcggcgtgat gggcgaaggt gctccaggtc ttctgcccgc tgatgaccca
      241 tcggtccccg tcccgcgtgg cccgggtgcg ggcgccggcc aggtccgagc cgccggtcgg
      301 ctccgagaac agctggcaga acaacgcctc gcccgccagc acctccggga tcagctggcg
      361 caggaacgcg ggctgcccgt gggccaggat ggtcggcacg ttgacgccgt aggtggtgat
      421 gatcagcggc ccgaagttgg gcgtgacgta gggctcggcc tcgtcgttga acgcggcctc
      481 gtaggcgtcg gggaggccct ggccgccata ctcggccggc cacgagatgc ccgcgtagcc
      541 ggcctcgtag agcttgcgct gcagggcccg gtcggccggc ttctggctgg cgtcgtagtg
      601 gtggaggctt cggcgggcgg gcgggccctc ccgccgggcc aggtttccag acagccaggc
      661 gcgggcccgg gcgcggtact cctccagatc ggggatctcc gtcgtcggta tcacagccgt
      721 cggtgtcaca gcccgtggac ctggcagatg cgctcccgat gccaatcggg gctgccgtac
      781 aggaggctgt tcatggtgat ccggcgcaga tagaggtggg agtcgtgctc ccaggtgtat
      841 ccgatgccgc cgaacacctg gaggcagccc tgggccagct ctattccggc gtctcccgtc
      901 catgccttgg cgatgctggc gacctccccg gcgtcggggc tgccggcgtc gaggacctcg
      961 acggcgttgg cggtgacggc ccgggccgcc tccagggaca ggctcatgtc ggccagctgg
     1021 tgcttgacgg cctggaacga cccgatggga cgcccgaagg ccgtgcggtc cagggcgtac
     1081 tggcgggcca tctcgaacag ggcatccatg gcaccgaccg tctccgcggc caccaggacc
     1141 gtggccagcc ggagctggtg ctcgacgtcg tcgtccgcac cgcccgccgg gcccaccagg
     1201 ctggtggcgt cgacatggac gccgtcgaag gtcacggtgg agaagcgcag ggtgacgtcg
     1261 tggcacgccc ggggcgccac gccggtcgcc gcgctccgcg ggaggaggaa ctggctgagg
     1321 ccctggccgc caccggcggt cacgaggagc cagtcgaccg aggcgccgtc ctgcacgagg
     1381 gcccggccag acagcgagta gccgtcgccg tcggcgcggg ccacgacagc cgtggctggg
     1441 gcgacgtcgc cggaggggtc ggcgaccacc catgacgcca cctgctcccc ggcggccagg
//
LOCUS       DASWGG010000109         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_753610, whole genome shotgun sequence.
ACCESSION   DASWGG010000109 DASWGG010000000
VERSION     DASWGG010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753610"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VF954_01990"
     CDS             <1..649
                     /locus_tag="VF954_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529881.1"
                     /translation="GRSGHGVRMRLRKAIPAGAGLGGGSADAAAAFAAVRRMLEVDIG
                     DEELSELGAALGSDVPFCLRGGAAWMRGRGERLEPVTLEGQLPLVVVLPPFRISTPDV
                     YRAWDELGGPSSTRIVPTPAALAGLGLPDGLVNDLEPAAEHIEPRLRPFREGLEALAG
                     GPALLAGSGSAYVVPVASSAQAAVVATQAGVELRATAFATGLAARGVEAQRGIDQ"
     gene            complement(655..756)
                     /locus_tag="VF954_01995"
     CDS             complement(655..756)
                     /locus_tag="VF954_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEX9529882.1"
                     /translation="MGSLIKKRRKRMRKKKHKKLLKRTRWQRRAAGK"
     gene            802..873
                     /locus_tag="VF954_02000"
     tRNA            802..873
                     /locus_tag="VF954_02000"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Gln,seq:ttg)
     gene            923..>1681
                     /locus_tag="VF954_02005"
     CDS             923..>1681
                     /locus_tag="VF954_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX9529883.1"
                     /translation="MVLAAGEGTRMKSSVPKVLHPLCGRPMLLYVIGALEELDLQRIV
                     VVVGHASERVIKTLHSETDVPLEFVEQRVQRGTGDAVSVGLTVFPDDMEEEDDLIVVP
                     GDTPLLRAETLADLAREHDESGAAATVLTARLPDPTGYGRIVRGKDGRVEAIVDHWDA
                     TEEERAIDEVNTSIYCFRRGLLVPALRRLSPENAQGEYYLTDAIAVLRQAGHKVVAME
                     SPEVAEAMGVNDQVQLAEAEQALRVRINSRWMRDG"
BASE COUNT          225 a          599 c          621 g          236 t
ORIGIN      
        1 cggacgctcc ggtcacggcg tgaggatgcg gctccgcaaa gccatccccg ccggagcggg
       61 actcggcggc ggctccgccg acgccgccgc cgcgttcgcc gcggtccgtc gcatgctcga
      121 ggtcgacatc ggcgacgagg agctcagcga gctcggcgcc gccctcggct ccgacgtccc
      181 gttctgcctg cggggaggcg cggcctggat gcgcggccgc ggcgagcgac tcgagccggt
      241 caccctcgag ggccagctcc cgctggtcgt ggtcctcccc ccgttccgga tctcgacccc
      301 cgacgtctac cgggcgtggg acgagctcgg cggcccgtcc tccacccgga tcgtccccac
      361 gccggcggcc ctggccgggc tcggccttcc cgacggcctg gtcaacgacc tcgagccggc
      421 ggccgaacac atcgagcccc ggctgcggcc gttccgggag gggctggagg cgctggccgg
      481 cgggccggcg ctgctggcgg gaagcggctc ggcctacgtg gtgccggtgg cgtcatcggc
      541 ccaggcggcg gtggtggcga cccaggccgg cgtcgagcta cgggccacgg cgttcgccac
      601 cgggctggcc gcccgaggag tcgaggccca gaggggcatc gaccagtaga acgcttactt
      661 cccggcggcg cggcgctgcc agcgggtgcg cttgagaagc ttcttgtgct tcttcttccg
      721 catgcgcttg cggcgcttct tgatcagaga gcccatggcg ggaggcaggt tagccgccgg
      781 gtgttggtat ggtcgattcg gtcgggggtg gtgtaaccgg cagcacaggg tcctttggag
      841 tccctagacg aggttcgaat cctcgccccc gagctggagg acggaaaggg gctcaatgac
      901 ccactcacga ccgttggccg ccgtggtcct cgccgccggc gagggcaccc ggatgaagtc
      961 gtccgtgccc aaggtgctgc atcccctgtg cggccggccg atgctgctct acgtcatcgg
     1021 cgcgctggag gagctggacc tgcaacggat cgtcgtcgtc gtgggccacg cctcggagcg
     1081 ggtcatcaag accctccaca gcgagaccga cgtccccctg gagttcgtcg agcagcgggt
     1141 ccagcggggg acgggcgacg ccgtctccgt cgggctcacc gtcttccccg acgacatgga
     1201 ggaggaggat gacctcatcg tcgttcccgg cgacacgccc ctcctgcggg ccgagacgct
     1261 ggccgacctg gcccgggagc acgacgagag cggcgccgcg gccaccgtgc tgaccgcccg
     1321 cctgcccgac ccgaccggct acggccggat cgtccggggg aaggacgggc gggtcgaggc
     1381 catcgtcgac cactgggacg ccaccgagga ggagcgggcc atcgacgagg tcaacacgtc
     1441 gatctactgc ttccgccggg ggctcctggt gccggccctg cgccggctgt cccccgagaa
     1501 cgcccagggc gagtactacc tgaccgacgc catcgccgtc ctccgccagg cgggccacaa
     1561 ggtcgtggcc atggagtcgc ccgaggtggc cgaggccatg ggggtcaacg accaggtgca
     1621 gctggccgag gccgaacagg cgctgcgggt ccggatcaac agccggtgga tgcgcgacgg
     1681 c
//
LOCUS       DASWGG010000110         1943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3009955, whole genome shotgun sequence.
ACCESSION   DASWGG010000110 DASWGG010000000
VERSION     DASWGG010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3009955"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1943)
                     /gene="pheT"
                     /locus_tag="VF954_02010"
     CDS             complement(<1..>1943)
                     /gene="pheT"
                     /locus_tag="VF954_02010"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX9529884.1"
                     /translation="VCGAWNFSVGDLVALAQVGTVLPGDFEITRRKMKGVSSDGMLCS
                     GRELRLTDDHEGILVLPPGLTPGQPFAEAMGIETDVVFDLDITPNRPDAMSVAGVARD
                     LAARMRVPFALPAAVVDETGPPTAGLTSVRVEDPELCPRFVARVLTGVRVQPSPAWLA
                     RRLTLAGMRPINSVVDASNYVMLELGQPTHPYDLGLVPGRGLVVRAASPGERLTTLDG
                     TERVLGGTPDARAADCLICDASGAPIGIAGVMGGASSEISAATTEVLLEAAYFQPMAI
                     ARTSKRLGLRSEASARFERGCDPQGIERAVARFCQLVAETSGPGVAVGMGVLDVRGSA
                     PGPLRLRLRTSRVNALLGTDLDDASVAAYLRPLGFGVQPAADGEAEVIVPTFRPDCER
                     EVDLIEEVARHHGYARIPKTVPTTAQVGHLTAAQRQRRAVRDVLVGAGANEAWTPSWL
                     APGDHARAGLPGKPVEIENPLSADESVLRRSMLPGLLRALAFNAGRRNPDVRLFEIGR
                     VFLDVPAGERLPDERELLSAALALPSDDAFAAVGVWRLLAGALRLAPVELRQDEVPGL
                     HPARSAALVAPASGVVVGTVGEVDPDIAAAHGVDGRVGWLEADLGLVLAAPHRSGQAT
                     DPSRFPSSDIDLAFAVDEGTPAA"
BASE COUNT          267 a          715 c          746 g          215 t
ORIGIN      
        1 cgccgcaggc gtgccctcgt ccacggcgaa ggccaggtcg atgtccgacg aggggaagcg
       61 gctggggtcg gtcgcctgcc cgctgcggtg cggagcggcc agcaccagcc ccaggtcggc
      121 ctccagccac cccacccgcc cgtcgacccc gtgggcggcg gcgatgtcgg ggtccacctc
      181 gccgacggtg cccaccacga ccccgctggc cggcgcgacc agggccgcgc tgcgggccgg
      241 gtgcagaccg ggcacctcgt cctggcgcag ctcgaccggc gccaggcgca gggcgccggc
      301 cagcaggcgc cagacgccga ccgcggcaaa ggcgtcgtcg gagggcagcg caagcgccgc
      361 ggacagcagc tcccgctcgt cggggagccg ctcgccggcc gggacgtcga ggaacacccg
      421 cccgatctcg aacagccgca cgtcggggtt gcgccgtccg gcgttgaagg ccagggcccg
      481 caggaggccg ggcagcatgg agcgtcgcag gaccgactcg tcggccgaca gcgggttctc
      541 gatctcgacc ggcttgccgg ggagcccggc ccgggcgtgg tcgccgggtg ccagccacga
      601 cggcgtccag gcctcgttgg cgcccgcacc taccagcacg tcgcgcaccg ctcggcgctg
      661 gcgctgggcg gcggtcaggt ggccgacctg ggcggtggtc ggcaccgttt tcgggatccg
      721 ggcgtagccg tggtggcggg cgacctcctc gatcaggtcc acctcccgct cgcagtcggg
      781 gcggaaggtg ggcacgatga cctcggcctc cccgtcggcc gccggctgca caccgaagcc
      841 gagggggcgg aggtaggcgg ccaccgaggc gtcgtcgagg tcggtgccga gcagggcgtt
      901 gacgcgggag gtgcgcaggc gcaggcgaag cggccccggc gcggacccgc gcacgtcgag
      961 gacccccatg ccgacggcga cgcccgggcc cgacgtctct gccaccaact ggcagaaccg
     1021 ggcgacggcg cgctcgatgc cctggggatc gcagccccgc tcgaagcggg ccgacgcctc
     1081 gctgcgcaac cccaggcgct tggacgtccg ggcgatcgcc atgggctgga agtaggcggc
     1141 ctcgagcagc acctcggtcg tggctgcgga gatctcgctc gaggccccgc ccatgactcc
     1201 cgcgatgccg atcggcgcgc ccgaggcgtc gcagatgagg cagtcggcgg cccgggcgtc
     1261 aggggtcccg cccagcaccc gctccgtgcc gtcgagggtg gtgaggcgct caccggggga
     1321 agcggcccgt accaccaggc cgcggccggg gaccaggccg aggtcgtagg ggtgcgtcgg
     1381 ctggcccagc tccagcatca cgtagttgga ggcgtccacc accgagttga tggggcgcat
     1441 cccggccagg gtcaaccggc gggccagcca ggccggcgac ggctggaccc gaaccccggt
     1501 gagcacgcgg gccacgaacc tcgggcacag ctcggggtcc tcgacccgca ccgacgtgag
     1561 gccggcggtc ggcgggcccg tctcgtccac gaccgccgcc ggcagggcga acggcacccg
     1621 catccgggcg gccaggtcgc gggccacccc ggccacggac atggcgtccg ggcggttggg
     1681 ggtgatgtcc aggtcgaaga ccacgtcggt ctcgatgccc atggcctcgg cgaagggctg
     1741 accgggtgtg aggccgggcg gcagcaccaa tatgccctcg tggtcgtcgg tgaggcgcag
     1801 ctcgcggccc gagcacaaca tgccgtcgga cgagacgccc ttcatcttgc ggcgggtgat
     1861 ctcgaagtcc cccggcagca ccgttccaac ctgggccagc gcgaccaggt cgccgaccga
     1921 gaagttccag gcgccgcaga cga
//
LOCUS       DASWGG010000111         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1394464, whole genome shotgun sequence.
ACCESSION   DASWGG010000111 DASWGG010000000
VERSION     DASWGG010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394464"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VF954_02015"
     CDS             <1..1221
                     /locus_tag="VF954_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9529885.1"
                     /translation="HDGHMLLRRAATLVALFDPDGAARAVTQLRADGVQRDVGIDLPP
                     EAEELRAVTRKVAEEIGALPKEAQRARLIDTGYVMPHWPAPWGRAAKALEQLVIDEEF
                     ARSRVSRPQYGITGWVILTLIQHATADQIERWVRPTLNGELVWCQLFSEPDAGSDAAG
                     IKTKATRVDGGFVISGQKVWTSGAQHCHRGLATVRTDPDKPKHSGITTVVIDMHAPGV
                     EVRPLREASGASMFNEVFFNDVFVPDDDVVGPVDGGWTVARSTLGNERVSIGGGQGGT
                     GAFTIDLLELYRDHGSRLDGAAIAVGANLAEQQTLKLLNLRAAERAVAGGEPGPEGNV
                     TKLVTAENMQRVADLALRLVGPQVAIQDGPGGLIAQVLIFSRAMTIAGGTSEITRNQI
                     GERILGLPRDPLIR"
     gene            1287..>2208
                     /locus_tag="VF954_02020"
     CDS             1287..>2208
                     /locus_tag="VF954_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9529886.1"
                     /translation="MFDLLIMGGTVVDGTGGPARRVDVAVDGERIAAVGTGLGPARRT
                     LDATGLVVTPGWVDIHTHYDGQVTWDQDVAPSSINGVTSLVMGNCGVGFAPARPDRHS
                     WLISLLEGVEDIPGTALSQGLTWGWESFPEYLDVLDQRRWTLDVGTQVPHAALRTYVM
                     GDRGADHTARADADEIASMSRMAEAAVRAGALGFTTSRTWAHRTSLGEQIGTLTASTE
                     EVLGIASALTRAGLGVVQLISDVYQSPDDELVERELRLLGSLALEIGRPMSFTVQQND
                     ETPDRFRQLLAAIGEWNAAGAVARAQVAVRP"
BASE COUNT          321 a          833 c          780 g          274 t
ORIGIN      
        1 cacgacggcc acatgctgct gcggcgtgcg gccacgctcg tggccctctt cgacccggac
       61 ggcgccgccc gggccgtcac ccagctgcgg gccgacggag tccagcgcga cgtcggcatc
      121 gacctgccgc ccgaggccga ggagctgcgc gccgtcaccc ggaaggtggc cgaggagatc
      181 ggggccctgc cgaaggaggc gcagcgggcc aggttgatcg acacgggcta cgtgatgccc
      241 cactggccgg cgccgtgggg gcgcgccgcc aaggcgctcg agcagctggt catcgacgag
      301 gagttcgccc ggtcccgggt ctcccgaccc cagtacggca tcaccggctg ggtcatcctg
      361 acgctcatcc agcacgccac cgccgaccag atcgagcgct gggtccgccc caccctcaac
      421 ggcgagctgg tctggtgcca gctgttcagc gagccggacg cgggctctga cgccgcgggc
      481 atcaagacca aggccacccg ggtggacggc ggcttcgtca tctccggcca gaaggtgtgg
      541 accagcggcg cccagcactg ccaccgcggc ctggcgaccg tgcgcaccga ccccgacaag
      601 cccaagcaca gcggcatcac caccgtcgtc atcgacatgc acgcccccgg cgtcgaggtg
      661 cggcccctgc gcgaggcctc gggcgcctcc atgttcaacg aggtgttctt caacgacgtg
      721 ttcgtccccg acgacgacgt cgtcggaccg gtcgacggcg ggtggacggt ggcccgatcg
      781 accctcggca acgagcgggt cagcatcggc ggcggccagg gcggcacggg cgccttcacc
      841 atcgacctgc tcgagctgta ccgggaccac ggctcgcgcc tcgacggcgc cgccatcgcc
      901 gtgggcgcca acctggccga gcagcagacg ttgaagctgc tgaacctccg ggccgccgag
      961 cgggcggtcg ccggcggaga gccggggccg gagggcaacg tcaccaagct ggtcaccgcg
     1021 gagaacatgc agcgcgtcgc cgacctcgcc ctgcgcctgg tcgggcccca ggtcgccatc
     1081 caggacgggc cgggcggcct catcgcccag gtgctgatct tcagccgggc catgaccatc
     1141 gccggcggca cgtccgagat cacccgcaac cagatcggcg agcgcatcct cggtcttccc
     1201 cgggaccccc tcatccggta gccggcgcct agcagggcag tggccggcgc gggcgccagg
     1261 cccggcgaga gggttacagt cgcaccatgt tcgacctgct gataatggga ggcacggtcg
     1321 tcgacggcac cggcggcccg gcgcgccggg tggacgtggc cgtcgacggc gaacgcatcg
     1381 cggccgtggg gaccggcctc gggccggcga ggcgcaccct cgacgccacc ggcctcgtcg
     1441 tcacccccgg ctgggtcgac atccacaccc actacgacgg ccaggtgacg tgggaccagg
     1501 acgtggcccc atcgtcgatc aacggggtga cgtcgctcgt catgggcaac tgcggggtgg
     1561 ggttcgcgcc ggcccggccc gaccgccaca gctggctcat ctccctgctc gagggcgtcg
     1621 aggacatccc cggcaccgcc ttgagccagg gcctcacgtg gggctgggag agctttcccg
     1681 agtacctcga cgtcctcgac cagcggcgct ggacgctcga cgttgggacc caggtcccgc
     1741 acgccgccct gcgcacctac gtgatgggcg accggggcgc cgaccacacc gctcgggccg
     1801 acgccgacga gatcgcgtcg atgtcccgga tggccgaggc cgccgtgcgg gccggcgcgc
     1861 tcggcttcac cacctcgcgc acctgggcgc accgaaccag cctgggcgag cagatcggca
     1921 ccctcacggc gtcgacggag gaggtcctcg gcatcgcgtc ggccctcacg cgggccggcc
     1981 tcggcgtggt gcagctgatc tccgacgtct accagtcccc agacgacgag cttgtcgagc
     2041 gcgagctgcg gctgctcgga agcctggccc tggagatcgg ccggcccatg agcttcaccg
     2101 tccagcagaa cgacgagacg cccgaccggt tccgccagct gctcgccgcc atcggggagt
     2161 ggaacgcggc cggcgcggtg gcccgggccc aggtcgccgt ccggccca
//
LOCUS       DASWGG010000112         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1395345, whole genome shotgun sequence.
ACCESSION   DASWGG010000112 DASWGG010000000
VERSION     DASWGG010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395345"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VF954_02025"
     CDS             <1..384
                     /locus_tag="VF954_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529887.1"
                     /translation="LAVAAAVGLAGGMLFLPRPGRPRATPALVARQAAIVTASGTRVG
                     TVALVSSPQPHVLVSLTDPRPGAGRLVCQLRAAGGAWVSVGSWTYDDIETGVWAVGVG
                     PSMLQAVAMRVLGAGGDVIATARLD"
     gene            complement(417..1403)
                     /gene="egtD"
                     /locus_tag="VF954_02030"
     CDS             complement(417..1403)
                     /gene="egtD"
                     /locus_tag="VF954_02030"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX9529888.1"
                     /translation="MVERLSVRVCLPADHRRVTLEHDLRTGLARRPKTLSPLWFYDER
                     GSALFDRITRLPEYYLTRAERSILDVAAGEIVGAAGCDQLVELGSGTSEKTRLLLDAM
                     SDAGRLRRFVPLDVSEATLREAAADISRSYGIAVHAVVGDFHQHLDCIPADGNRLVAF
                     LGSTIGNLDPGERSAFLRALAATLGPGDRLLLGTDLVKDPGRLLAAYDDRAGVTAEFN
                     RNMLRVLNAEAGGDFDPDQFDHLAVWDEASHWIEMRLRSRSPQTVRLARFGLEVSFRE
                     GEDLRTEISSKFTAEQVEMELWGAGLAVEQTWTDPDKDFQLSLSRLAGQGRS"
     gene            complement(1413..>1715)
                     /locus_tag="VF954_02035"
     CDS             complement(1413..>1715)
                     /locus_tag="VF954_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX9529889.1"
                     /translation="LGPYVGGRDELAYHPGPLTEDPPSVGAVWEWTASAYLPYPRFAP
                     APGAVGEYNGKFMVNQHVLRGGCCATPPGHLRPSYRNFFPPAARWPFTGLRLARNL"
BASE COUNT          237 a          632 c          625 g          221 t
ORIGIN      
        1 ctcgccgtgg ccgcggcggt cggcctcgcc ggcgggatgc tctttctccc acgccccggc
       61 cgcccccggg ccacccccgc cctggtggcc aggcaggccg cgatagtcac ggcgagcggg
      121 acgcgtgtcg gcacggtggc cctggtgagc agcccgcagc cccacgtcct ggtcagcctc
      181 accgaccccc ggcccggcgc gggccggctg gtctgccagc tccgggcggc gggcggggcg
      241 tgggtctccg tcgggagctg gacctacgac gacatcgaga ccggggtctg ggcggtcggc
      301 gtgggcccgt cgatgctcca ggccgtggcc atgcgcgtgc tcggcgccgg gggcgacgtg
      361 atcgccaccg cccgcctgga ctgaccgacc gacctaccgg ccgacgacct gacggctcac
      421 gacctaccct ggccggccag gcgggacagc gagagctgga agtccttgtc cgggtcggtc
      481 cacgtctgct ccaccgccaa cccggccccc cacagctcca tctccacctg ctcggccgtg
      541 aacttggagg agatctccgt gcgcaggtcc tcgccttccc ggaacgagac ctccagaccg
      601 aacctcgcca gccggacggt ctgcggcgag cgggagcgga ggcgcatctc gatccagtgg
      661 ctggcctcgt cccacacggc gaggtggtcg aactggtcgg ggtcgaagtc gccgccggcc
      721 tccgcgttca ggacccgcag catgttgcgg ttgaactcgg ccgtgacgcc ggcgcggtcg
      781 tcgtaagcgg ccaggagccg cccggggtcc ttgaccaggt cggtgccgag cagcagccgg
      841 tcacccggcc cgagcgtggc ggccagggcg cgcaggaagg cgctccgctc cccggggtcg
      901 aggttgccga tggtggagcc gaggaacgcc accagccggt tgccatccgc ggggatgcag
      961 tccaggtgct ggtggaagtc ccccaccacg gcgtgcacgg cgatgccgta cgaccggctg
     1021 atgtccgccg ccgcctcgcg cagggtcgcc tcgctgacat cgagagggac gaaccggcgc
     1081 agccggccgg cgtcgctcat ggcgtcgagc aggaggcggg tcttctccga cgtccccgag
     1141 ccgagctcga ccagctgatc gcacccggcc gccccgacga tctcgccggc ggcgacgtcg
     1201 aggatggacc gctcggcccg ggtcaggtag tactcgggca ggcgggtgat ccgatcgaac
     1261 agggcgctgc ccctctcgtc gtagaaccac aagggggaca aggtcttggg gcgccgggcc
     1321 aggccggtcc gcagatcgtg ctccagggtc acccgccggt gatcggcggg caggcacacg
     1381 cgcaccgaca agcgctcaac catcgccgcc ccctagaggt tcctggccag ccgcagcccg
     1441 gtgaaaggcc agcgggcggc gggagggaag aagttcctgt agctgggccg caggtgcccg
     1501 ggcggggtgg cacaacagcc tccccgcagc acgtgctggt tgaccatgaa cttgccgttg
     1561 tactcgccga ccgcgccggg agcgggggcg aaccgcgggt acggcaggta cgccgaggcc
     1621 gtccactccc acaccgcccc caccgacggc gggtcctcgg tgagcggccc ggggtggtag
     1681 gcaagctcgt cccgccctcc gacgtacggc cccag
//
LOCUS       DASWGG010000113         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2688978, whole genome shotgun sequence.
ACCESSION   DASWGG010000113 DASWGG010000000
VERSION     DASWGG010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2688978"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..370)
                     /locus_tag="VF954_02040"
     CDS             complement(41..370)
                     /locus_tag="VF954_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529890.1"
                     /translation="MSDDQHRVSDEHRPSLGPDLVEVHFRALRHALRAKRVSNLSDLS
                     LLLASDGVAVADAVVLLDKAASGDRRAVELAQLHVGAIAAHAPRSHAPAGAALLLLAV
                     ERLTASN"
     gene            433..>1929
                     /locus_tag="VF954_02045"
     CDS             433..>1929
                     /locus_tag="VF954_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9529891.1"
                     /translation="MRHVRGARRLARPLALAVAAGLGAPGCSGGPSGAHRHATATTAA
                     TSTTAAASTKGTAASTKGTAASTTGTATTATTATTRAGAPTGADWVTYQHDPARTGRT
                     ADGPGTTQIAQRWATPMGADVYAQPLVVGSQVIVATEADQVAALAAADGHVVWRTSIG
                     TPVPRSALPCGDIDPSGVTSTPAVDRAAGIVWVAEETTSPAQPGQVHHDLVGLDLATG
                     AIKSRRTIDPPGSDPAAQQIRAAVTLWGSTVYVPIGGLFGDCGAYRGYVVGVREDGTG
                     GLAVWQVSDPGGGGGVWTTSGATVDDTGLLVATGNSRSTSSFADGNAVVRLTAGLQKT
                     DEFAPTNWAGLSSTDTDLNSTGPAVVGDSVVQVGKEGVGYLLDRHHLGGIGGQVATAA
                     VCTAFGGTAQLDGVVFEPCTSGLAAVSVGSRTLVRSWTAGGYDGGTPIVVGNVVWQID
                     VTNGRLLAYTAATGTRLTDVAVGPVHHFAPMAAGDGLLLVAGRDRILAF"
BASE COUNT          254 a          691 c          734 g          250 t
ORIGIN      
        1 ccgggtggct cgttggtggc tcgttggtgg ctcgttggtg ctagttgctc gcggtcaggc
       61 gctccaccgc gagcaggagc agggcggccc cggcgggggc gtgtgaccgc ggggcgtgcg
      121 cggcgatggc cccgacgtgg agctgggcca gctcgacggc ccgccggtct ccggaggccg
      181 ctttgtcgag caggaccacc gcgtcggcga cggccacccc gtcgctggcc agcaacaggg
      241 acagatcgct caggttcgag acccgcttcg cccggagcgc atgccgcagc gctcggaagt
      301 ggacctcgac gagatcaggg ccgagagatg gtcggtgctc gtcgctcacc ctgtgttgat
      361 cgtcgctcac gccttgttga ttcgacgctc gcagcgctcg cccctgctcg gtgagcgggc
      421 aggatggagg tcatgcgaca cgtccgcggc gcccggcgcc tggcccggcc cctggcactg
      481 gccgttgccg ccgggttggg ggcgccgggc tgctcgggcg ggccttcggg tgcccaccgg
      541 cacgccactg cgacgaccgc ggccacctcg accacggcgg ccgcctcgac caagggcacg
      601 gccgcctcga ccaagggcac ggccgcctct accacgggca cggccaccac ggccaccacc
      661 gccaccacca gggcaggggc gccgaccggg gcggactggg tgacctacca gcacgacccg
      721 gcccgaacgg gccggaccgc cgacgggccg ggcaccaccc agatcgccca acgctgggcg
      781 acaccgatgg gcgccgacgt gtacgcccag cctctcgtgg tgggcagcca ggtgatcgtc
      841 gccaccgagg cggaccaggt ggcggccctg gcggcggccg acgggcacgt ggtgtggcgc
      901 acgtccatcg ggactcccgt tccccgcagc gccctgccct gcggcgacat cgacccgagc
      961 ggcgtcacct ccacgccggc ggtcgaccgg gcggcgggca tcgtctgggt cgccgaagag
     1021 accacgagcc ccgcccaacc cgggcaggtc caccacgacc tggtcggcct ggacctggcg
     1081 acgggagcga tcaagtcccg gcgcaccatc gacccacccg ggtcggaccc ggcggcgcag
     1141 cagatccggg ccgcggtgac gctgtggggc tcgaccgtgt acgtgccgat cggcgggctc
     1201 ttcggcgatt gcggcgccta tcggggttac gtcgtcggcg tgcgggagga cgggaccggc
     1261 gggctcgccg tgtggcaggt ttccgacccc gggggcggag gcggtgtgtg gaccacgtcg
     1321 ggcgcgaccg tcgacgacac cgggctgctg gtggccacgg ggaacagccg ctcgacgtcg
     1381 tccttcgccg acgggaacgc agtggtgcgg ctgacggcgg gattgcagaa gaccgacgag
     1441 ttcgccccga cgaactgggc cgggctgagc agcacggaca cggatctcaa cagcaccggc
     1501 ccggccgtgg tgggcgactc cgtggtccag gtcggcaagg agggcgtcgg ctacctcctc
     1561 gaccggcacc acctcggggg catcggtggg caggtcgcca cggcggcggt gtgcacagcc
     1621 ttcggcggga cggcccagct cgacggcgtg gtgttcgagc cgtgcaccag cggcctggcc
     1681 gcggtcagcg tgggcagccg gacgctcgtg cggtcctgga cggccggtgg ttacgacggt
     1741 ggcaccccca tcgtggtggg caacgtcgtg tggcagatcg acgtgaccaa cggccggctc
     1801 ctcgcctaca cggcagccac cggcaccagg ttgacggacg tggcggtcgg tcccgtccac
     1861 cacttcgccc cgatggcggc cggcgacggc ctgctcctgg tggcgggcag ggaccggatc
     1921 ctggccttc
//
LOCUS       DASWGG010000114         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2689830, whole genome shotgun sequence.
ACCESSION   DASWGG010000114 DASWGG010000000
VERSION     DASWGG010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2689830"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VF954_02050"
     CDS             <1..778
                     /locus_tag="VF954_02050"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9529892.1"
                     /translation="HNIESQRVPENGSASRRPDRGWERRRYRRLESRLAPGSGPLAVT
                     VSERDARSIGHGIVVPNGADLPATPPPLDPDGPALFVGALDYRPNAEALRWFVERVAP
                     HLGRGRLPLQVVGRAASALPADLARHPALDVVGEVDDVGPYLGAAGIVVAPLREGGGT
                     RLKVVEALAWGRPVVTTTKGAEGLELVAGRDALFADDAPSFAAAMELLATDHACARRM
                     AAAGRRAVQPYSWDVIGAAFAGAVLARAEPWRRGSRGRVG"
     gene            775..>2172
                     /locus_tag="VF954_02055"
     CDS             775..>2172
                     /locus_tag="VF954_02055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9529893.1"
                     /translation="MKAAPEPDEPRARVLAWPAWRTGTNPYTALLSDALEANGVAVEE
                     LSPRRALAARDGVVHVHWPEAFLDRYGLPRAALQAAAQLAVLSCTRRRGNRVVWTVHN
                     LGSHDRRHPRLEGWYLDRFAGLVDGWISLSHAAIDPIVAAFPPLDARPRRVIPHGHYR
                     RADAEPLTRSEARAQLDLPADGAIVLSFGALRDYKGIPDLIRAFRAFGALRAVGEAGA
                     VLRVVGEGDRAVVRACLAAAAGDPSVTITERFVPEPEVSAHFASADLVALPYLRGLNS
                     GAALLALSCNRPVLVPDTPAFRELQAMVGREWVRTFEPPLRAGALAAALDEVRDRTGC
                     EKLDLTPFDLAGIAASTASFYEEMAGARPVTVTACFEPAAVVGGLERSVQATVEPLRA
                     RGYGVRALVNAARPSLTASSLACASPVASTARLRGVVGAVRASDVVHLHAPTTARWSM
                     RTVMVAAALRRRPVLT"
BASE COUNT          241 a          826 c          812 g          293 t
ORIGIN      
        1 gcacaacatc gagtcgcaac gggttcccga gaacggttcg gcgtcccgcc gccccgacag
       61 ggggtgggag cgccggcggt accgccggct ggagtcccgt ctggcgccag gttcgggccc
      121 tctggccgtc accgtctccg aacgcgacgc ccgatcgatc ggccacggga tcgtcgtgcc
      181 caacggtgcg gacctccccg cgaccccgcc gcccctcgac cccgacgggc ccgccctgtt
      241 cgtcggagcg ctggactacc gccccaacgc cgaggcgctg cgctggttcg tggagcgcgt
      301 ggcaccccac ctgggccggg ggcgcctccc gctccaggtg gtgggccggg cggcctcggc
      361 gctgcccgcc gacctggccc ggcacccggc gctcgacgtg gtgggggagg tcgacgacgt
      421 aggcccctac ctgggcgccg ccgggatcgt ggtcgcgcca ctgcgggagg gcgggggcac
      481 gcgcctgaag gtcgtcgaag ccctggcttg ggggaggccc gtcgtgacga ccacaaaagg
      541 agccgagggc ctcgagctcg tagccggccg ggacgccttg ttcgcggacg acgcgccatc
      601 cttcgcggcc gccatggagc tgctggcgac cgaccacgca tgcgcccggc ggatggccgc
      661 cgccgggcgc cgggccgtcc agccctactc gtgggacgtc ataggagcgg ccttcgccgg
      721 agccgtcctg gcccgagccg agccgtggcg gcgagggtcg cgcgggcgcg tcggatgaaa
      781 gccgcaccgg agcccgacga gccccgcgcc cgggtcctgg cgtggccggc gtggcggacc
      841 ggaaccaacc cgtacaccgc gctgttgagc gacgccctgg aggccaacgg ggtcgcggtc
      901 gaggagctct cgccccgccg ggcgctggcc gcccgcgacg gcgtggtcca tgtgcactgg
      961 ccggaggcct tcctcgatcg gtacggcctg ccccgcgccg ccttgcaggc cgccgcccag
     1021 ctggctgtcc tgtcgtgcac ccgccgccgc ggcaaccggg tggtgtggac cgtgcacaac
     1081 ctgggctccc acgaccgccg ccacccccgt ctcgagggct ggtacctgga ccgcttcgcc
     1141 gggctcgtcg atggctggat cagccttagc cacgccgcca tcgacccgat cgtggcggcc
     1201 ttccctccgc tcgacgctcg accgcgccgg gtgatccccc acgggcacta ccggcgcgcc
     1261 gacgcagagc cgctcaccag gtccgaggcc cgcgcccagc tcgacctgcc tgccgacggg
     1321 gcgatcgtgc tgtcgttcgg cgcgctgcgg gactacaagg gcatcccgga cctgatccgg
     1381 gccttccggg ccttcggggc cctccgggcg gtcggcgagg ccggcgccgt gctccgggtc
     1441 gtgggcgagg gcgaccgggc cgtggtgcgg gcgtgcctgg cggcggcggc tggcgaccct
     1501 tcggtgacca tcaccgaacg gttcgtccca gagccggagg tcagcgccca cttcgcctcc
     1561 gccgatctcg tggcgctgcc ctacctgcgc gggctcaact cgggtgcggc cttacttgcc
     1621 ctgtcgtgca accggccggt gctggtgccc gacacgccgg ccttccgcga gctccaggcg
     1681 atggttggcc gtgagtgggt gcgcaccttc gagcctccgc tgcgggccgg agcgctcgcc
     1741 gccgcgctgg acgaggtgcg ggatcggacg ggctgcgaga agctggatct gacgccgttc
     1801 gatctggcgg ggatcgcggc gagcacggcc tcgttctacg aggagatggc cggcgcccgc
     1861 cccgtgaccg tcacggcgtg cttcgagccg gccgcagtcg tgggcgggct cgagcgcagc
     1921 gtgcaggcca cggtcgagcc gctccgggcg cggggctacg gcgtacgcgc cctggtcaac
     1981 gcggcccggc cgtcgctcac cgccagcagc ctggcctgcg cctcgcccgt cgcgtcgacc
     2041 gcccggctgc gaggcgtggt aggggccgtt cgcgccagcg acgtggtcca tctccacgcg
     2101 cccaccacgg cgcgctggtc gatgcgcacg gtcatggtgg ccgcggcgtt gcggaggcga
     2161 cccgtgctga cc
//
LOCUS       DASWGG010000115         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1397535, whole genome shotgun sequence.
ACCESSION   DASWGG010000115 DASWGG010000000
VERSION     DASWGG010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397535"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /locus_tag="VF954_02060"
     CDS             complement(<1..1686)
                     /locus_tag="VF954_02060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9529894.1"
                     /translation="MALATAGALGGAATAAVVVEGRWIGRALFGARYASIGHLAFPYM
                     GAMALFGMCRVVVAERCGRGQARSTGVLLGAVAAGHALLILAVGTDPARIAAATLAAC
                     AALTAGLLVLSPPRRAWGSARGRATLVALPTLPDPVRDRPPRVTPDHLPAPDRRWIRT
                     VAVLAAMSVVALAVRLAVPRGLWLDEATSVLEARLPFSGMLRTLRTGDVHPPLYFSML
                     WLAVRAVGSGEVAVRLPSMLAGVAVVPAAFVTARDLYDRRTGMVAAALAVGAPLLVWY
                     SQEARMYSLFMLLSLLAVWGQVRALRYGRPRDWALYVAASSMLVWNEYFGLLQVVAQQ
                     AGFGWVAWVCRSARDRRDYLVGWALASLALVAMLLPLAPLALHQFRVNQHAGLGFTQV
                     PSQAGNGASAVRSTRLSIYALGANLIWALWGYHADTTIALIAALWPLAMLGSLVLLGR
                     RRRELTTVVLMSVIGPTAALFLLGLLKPELFDVRYLAGVVVLLVVLMARAVTGLSRTG
                     AVIGVVTVLLLATNGAALADQQLNGANPRRYDFRSAFARIRADARPGDLVVYDP"
BASE COUNT          287 a          605 c          664 g          152 t
ORIGIN      
        1 ggggtcgtag accacgaggt ctcccggccg ggcgtcggcg cggatgcgcg cgaacgcgga
       61 gcggaagtcg tagcggcggg ggttggcccc gttgagctgc tggtcggcga gggccgcccc
      121 gttggtcgcc aacagcagga cggtcaccac cccgatcacc gcgccggtgc ggctcaggcc
      181 ggtgacggca cgggccatga gcaccacgag caggacgacc acgccggcca ggtagcgcac
      241 gtcgaagagt tccggcttga gcaggccgag caggaacagg gcggcggtgg ggccgatcac
      301 cgacatgagg accaccgtcg tcagctccct gcggcggcgg cccagcagca cgagcgagcc
      361 gagcatggcg aggggccaga gcgcggcgat gagcgcgatc gtcgtgtcgg cgtggtagcc
      421 ccacagcgcc cagatgaggt tggcgcccag ggcgtagatc gacaggcggg tggagcgcac
      481 ggccgaggcg ccgttgcccg cctgggacgg aacctgggtg aaccccaacc cggcgtgctg
      541 gttgacgcgg aactggtgca gcgcgagcgg ggcgaggggc aacagcatcg ccaccagagc
      601 caggctcgcc agcgcccagc cgaccaggta gtcgcgccgg tcccgggccg agcggcacac
      661 ccacgccacc cacccgaagc cagcctgctg ggcgacgacc tggagcaggc cgaagtactc
      721 gttccagacg agcatggagc tggcggccac gtacagcgcc caatcccggg gccggccgta
      781 ccgcagggcc cgcacctggc cccagacggc cagcagtgaa aggagcatga acagcgagta
      841 catgcgggct tcctgcgagt accacaccag gaggggggcg ccgacggcca gggcggccgc
      901 gaccatgccg gtccggcggt cgtacaggtc gcgggcggtc acgaaggccg cgggcaccac
      961 cgccaccccg gccagcatgg acggcagccg cacggccacc tcgccactgc cgaccgcccg
     1021 gacggccagc cacagcatgg agaagtacag aggggggtgc acgtcgccgg tgcgcagcgt
     1081 gcgcagcatg cccgagaacg gcagcctcgc ctcgaggacg ctggtcgctt cgtcgagcca
     1141 caggccgcgg ggtacggcca gtcgcacggc cagagccacc acgctcatgg cggcgaggac
     1201 ggcgaccgtc ctgatccagc ggcggtccgg ggcgggcagg tggtccggcg tgacgcgggg
     1261 aggacggtca cggacggggt cgggaagggt ggggagggcg acgagggtgg cccgtccccg
     1321 ggcggaaccc caggcgcgcc gggggggaga aagcacgagc agcccggcgg tcagcgccgc
     1381 gcaggcggcg agggtggcgg ccgcgatccg cgccgggtcg gtgcccaccg ccaggatgag
     1441 cagggcgtgg cctgccgcga cggccccgag cagcacgccc gtcgaacgag cctggccgcg
     1501 gccacagcgt tccgccacca ccacccggca catcccgaac aacgccatgg cccccatgta
     1561 cgggaaggcc aggtggccga tcgaggcgta gcgagcgccg aagagcgccc gcccgatcca
     1621 gcggccctcg acgacgaccg ccgcagtcgc cgccccgccg agcgcgccgg ccgtggccag
     1681 cgccaccgcc aggacgtggc cgtggcgg
//
LOCUS       DASWGG010000116         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_758247, whole genome shotgun sequence.
ACCESSION   DASWGG010000116 DASWGG010000000
VERSION     DASWGG010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758247"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VF954_02065"
     CDS             complement(<1..630)
                     /locus_tag="VF954_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX9529895.1"
                     /translation="MAPAPFVDPDEIRRITRQVLSRRELSRPGPSIVQRVEGVIGRAF
                     ERAFGAALGGHGSLLASLFLLCILAVLLLLVLRFATTLRRQSAFPEPHGGSLARPATD
                     WDADAAAHERAERWRDAVRCRYRASVAALAALGLVEERDGRTAGEYRRSVDRDVAAVA
                     GAFDRLTDLFELTWYGDRAAGPADATQAARLRAGVESSARRASRRATRHE"
     gene            complement(637..1581)
                     /locus_tag="VF954_02070"
     CDS             complement(637..1581)
                     /locus_tag="VF954_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529896.1"
                     /translation="MTVSDLLDGAFKLYKANFGSILVVDLVFVAPFQLAAAFAQRNTF
                     GGQSFLRVFSSPSTLSAQRGSSPGSTIARGVVLLLDLLLVPFVAGAISRIVGASYLGQ
                     EISAGPALRATARRWLSLLAAWVMIHLMELGGLVMGLLFVGVAVAFSGPGRVIGVLVA
                     VGAFLVSGPVALAVMALSVMVAPAIVAEDLGPIRGIRRSWRLVRRRFWPVLGTALLAG
                     FMASILGSVIGAGPQIAAFGIGLKWGWIILGAGSVVVSALTLPIVAITATLLYFDARI
                     RTEGFDLQVLAMNLASPIQRPIQPPFQPPSRPPTSPVG"
     gene            complement(1883..>2665)
                     /locus_tag="VF954_02075"
     CDS             complement(1883..>2665)
                     /locus_tag="VF954_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529897.1"
                     /translation="LGARRAGLSCLMLDRLPGPEELAAYRPTVLAGRPGDLMRALQAA
                     RRGARLDRLGTVLSVGASEADRLDDGRRGRLQELAGTGAEVVQAWAPPGVRAMWAQCR
                     GGRGLHTWPMTELVQLVDPLSAAPVPPGADGEVVWTSLGWRGTTLLRLRTGLYGLVDE
                     GPCPTCGRTTPRILPTDRMPAFAAVLDARTEVSAWQAELRTVMGREELVVYLTPAPGG
                     NPSSLVRDLGRQLSATKFVVLDQIELDARLAAHGDARLVDLR"
BASE COUNT          400 a          981 c         1031 g          253 t
ORIGIN      
        1 ctcgtggcgg gtggcccgcc ggctggcgcg ccgcgccgag ctctccaccc cggcgcggag
       61 ccgggccgcc tgcgtggcgt cggcggggcc ggcggcccgg tcgccgtacc acgtgagctc
      121 gaacagatcg gtcaggcggt cgaacgcgcc agccacagcg gcgacgtcgc ggtcgacgct
      181 gcggcggtac tcgcctgccg tcctgccgtc gcgctcctcg accagcccca gggccgcgag
      241 cgcggcgacc gacgcccggt agcggcaccg cacggcgtca cgccagcgct ccgcccgctc
      301 gtgggcagcg gcgtcggcgt cccagtcggt cgccggtcgc gctagggacc cgccgtgggg
      361 ctcggggaag gcggattggc gccggagggt cgtggcgaag cgcagcacca gcagcagcag
      421 gaccgccagg atgcagagga ggaagagcga ggcgagcagc gagccgtggc ccccgagcgc
      481 ggcgccgaag gcacgctcga aggctcgacc gatgacgccc tcgacccgct ggacgatgga
      541 cgggcccggc cggcttaact cgcgacggga cagcacctgc ctggtgatgc gacggatctc
      601 gtcgggatcg acgaacggcg cgggcgccaa ggcggctcag cccaccggtg aggtcggcgg
      661 tcgcgacggc ggctggaacg gcggctggat cggccgctgg atcggcgagg cgaggttcat
      721 ggccagcacc tggaggtcga agccctccgt acggatgcgg gcgtcgaagt acagcaaggt
      781 ggcggtgatg gcgacgatcg ggagggtgag ggcggacacc acgaccgacc cggccccgag
      841 gatgatccag ccccacttga gcccgatgcc gaaggccgcg atctgggggc ccgccccgat
      901 caccgacccg aggatcgagg ccatgaagcc ggccagcaga gccgtgccca ggaccggcca
      961 gaaccgccgc cgtaccagtc gccaggaccg ccggatgccg cggatggggc ccaggtcctc
     1021 ggcgacgatg gccggggcca ccatgaccga cagcgccatc acggccagcg ccaccggccc
     1081 gctcaccagg aacgccccga cggcgacgag gacaccgatc acccgcccag gccccgagaa
     1141 ggcgacggcc accccgacga agaggaggcc catcaccagg ccaccgagct ccatcaggtg
     1201 gatcatcacc cacgccgcca ggagcgacag ccaccgccgg gccgtggcgc gcagggccgg
     1261 gccggccgag atctcctggc cgaggtagga ggcgcccacg atccggctga tggcgccggc
     1321 cacgaacggc acgaggagca ggtcgaggag gagaaccacg ccccgggcga tggtcgagcc
     1381 cggggagctg ccgcgctggg ccgacagcgt cgagggactg gagaacaccc gcaggaagct
     1441 ctgccccccg aaggtgttgc gctgggcgaa cgcggcggcc agctggaacg gggcgacgaa
     1501 caccaggtcg accaccagga tcgacccgaa gttggccttg tacagcttga aggccccgtc
     1561 caggaggtcg ctcacggtca tggggtggag gggcaggggg ccggtgcgaa cgctgcccgc
     1621 cttgggcccc gcaggagggg ggcggtagcc cggagggccc cagccggggg gcgggggcgg
     1681 cgggccccag ccggagggcg gggggggcgg gccccagccg gggggcgggg gcggcggcgg
     1741 ccgttcgggt gcgtcgccgt cgacgggcac ccagggcggg ggcggtgaac ggtcgggttc
     1801 gtcgggcgcc acggcgccac ccatcatgac agccccggcc gaccggccgg cgcgccgccc
     1861 ggccggggcg cgggccggcg cctcagcgga ggtccaccag ccgggcgtcg ccgtgggcgg
     1921 cgaggcgggc gtcgagctcg atctggtcga gcaccacgaa cttggtggcc gacagctggc
     1981 gaccgaggtc gcgcaccaac gaggagggat tgcctcccgg cgccggcgtg aggtacacga
     2041 ccagctcctc ccgacccatc accgttcgca gctcggcctg ccacgcggac acctcggtcc
     2101 tggcgtcgag gacggcggcg aacgcgggca tgcgatcggt cggcagtatg cgtggcgtcg
     2161 tccggccgca cgtcgggcac gggccctcgt ccaccaggcc gtacagcccg gtccgcagcc
     2221 ggagcaacgt cgtgccgcgc cagcccaggc tggtccacac cacctcgccg tcggcgcccg
     2281 gaggcacggg tgcggccgac agcgggtcca cgagctgcac cagctcggtc atcggccagg
     2341 tgtgcagtcc ccgacccccc cggcattggg cccacatggc acgcacgcca ggcggcgccc
     2401 aggcctgcac cacctcggcc ccggtcccgg ccagctcctg cagccggccc cggcggccgt
     2461 cgtcgagacg gtcggcctcg gacgcaccga ccgacagcac ggtgccgagc cggtccaggc
     2521 gcgccccgcg gcgcgctgcc tggagagcgc gcatgaggtc gcccggcctt cccgccagca
     2581 ccgtcggccg gtaggccgcc agctcctccg gaccgggaag gcggtcgagc atgagacagg
     2641 acagccccgc ccggcgcgcc cccag
//
LOCUS       DASWGG010000117         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1084127, whole genome shotgun sequence.
ACCESSION   DASWGG010000117 DASWGG010000000
VERSION     DASWGG010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084127"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VF954_02080"
     CDS             <1..218
                     /locus_tag="VF954_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529898.1"
                     /translation="PTLDPALARDDALAYLRQVGFGGRVLAVSVSPDDRQVQVSIEQT
                     RPTSLLRLFGVGRLTVHAVGTASPREG"
     gene            238..>2277
                     /locus_tag="VF954_02085"
     CDS             238..>2277
                     /locus_tag="VF954_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX9529899.1"
                     /translation="MRIVAGAGLVATALAWPVALGALGHPFTTPHLLNINQVRHFLSS
                     SDPQATGRAVAAALSRVAWLAWAYLIAVFGLGALSRAVRHVDCLYRALTPAPLRNLVV
                     TPLLALVISTGGRGSAPPPPAHLQAPATAPGAPGAPVVPGRPVSLPGAAAAVPGAPGA
                     SGGSDPGTSSAALAARTYLVRPGDSVWRIAERECASPLLWRQIVDLNLGSAQPGGEVF
                     RDPHWIRAGWTLRLPAACDPGEGAEYVVAPGESLSLIAAREYGDASMATAIWEANRGR
                     AMGDGRRFESPNLVYPGWVLSLPARTSASGASTTPHTATPVPGSSQAPTPAPSSTHAT
                     APAGRTPPAAPTPARGVPSPVPAGGVATPAPSLASTVPPPQGGQGSGAGEATPAGGGP
                     ASHQRGAQSQHRWRIPASGVVLPGSVASLLAAGLVLARIQRRRRWRPGESPAPVPEGP
                     VAGEVAASGAVPSLDLLGALSARVLAAWNQAVGSVPRILAAWEAPGAVRFLLSDDHPE
                     SALPEPVELQGVEVGFHRRDGALWAEARGEAEKALRRNLLVLADGLLVPVAHHAGGGC
                     PPGGGVGRAALHLPVLGTPLWVSGAGAAAMVESILIAAGLRAGAGDLRVVAVGDVPPL
                     LEEARVPDALAFPPIERPSASELEAVRTRLERALVLRAGRLAQAEAEGIEEYALHH"
BASE COUNT          236 a          888 c          850 g          303 t
ORIGIN      
        1 tcccgaccct cgatcccgcc ctggcacggg acgacgccct cgcctatctc cggcaggtcg
       61 gctttggggg cagggtcctg gccgtgagtg tcagccccga cgaccggcag gtccaggtga
      121 gcatcgagca gacccggccg acctccctcc tgcgcctgtt cggggtggga cgcctcacgg
      181 tgcatgccgt gggcaccgcc agcccgcgtg agggctgagg cgatgggcgg caggggagtg
      241 aggatcgttg cgggggcagg gcttgtcgcc accgcgctcg cgtggcctgt tgccctcggc
      301 gccctcgggc atcccttcac gacgccgcac ctgttgaaca tcaatcaggt acgccatttc
      361 ctctcgagct ccgaccccca ggcgacgggc cgggcggtgg ccgccgccct ctcccgggtg
      421 gcgtggctgg cctgggcgta cctcatcgcc gtcttcggcc tgggagccct ctcgagggca
      481 gttcgccacg tcgactgcct gtaccgcgcc ctcacccccg ccccgttgcg caacctcgtg
      541 gtgacccccc tgctcgccct ggtgatctcc acgggcggcc gaggatctgc gccgccaccc
      601 cctgcccatc tgcaggcgcc ggccacggcc ccgggagccc cgggagcccc cgtcgtcccc
      661 ggccggcccg tctccctccc cggggccgca gcagctgttc caggggcgcc cggcgcctcc
      721 ggcggttcgg atccgggtac ctcctcggcg gcgctggcgg cgaggaccta cctggtgcgc
      781 cctggtgaca gcgtgtggcg catcgccgag cgtgagtgcg ccagtccgct gctgtggcgc
      841 cagatcgtgg acctgaatct cgggagcgcc cagcccggcg gggaggtctt cagagacccg
      901 cactggatac gggcggggtg gaccctgcgg ctgccggcgg cctgcgaccc gggagagggc
      961 gccgagtacg tcgtggcacc cggcgagtcg ctcagcctga tcgcggcccg ggagtacggc
     1021 gacgcgtcga tggccaccgc catctgggag gcgaaccggg gccgggcgat gggcgacggg
     1081 cggaggttcg aaagccccaa cctcgtctac cccgggtggg tgctgtcgct gccggccagg
     1141 acctcggcct ccggagcctc gacgaccccg cataccgcga cgcctgtacc ggggtcctcc
     1201 caggcgccga cccccgcacc gtcgtcgaca cacgccacgg ccccggccgg ccggaccccg
     1261 ccggcagcgc ccacgccggc ccgtggcgtc ccgtccccgg tcccggccgg tggcgtcgcc
     1321 acgccggcgc cgtccctggc ctccacggtc cccccaccgc aggggggcca gggctccggt
     1381 gccggcgaag ccacccccgc ggggggcggt cctgcctccc accagcgtgg cgcccagtcg
     1441 cagcatcgct ggcggatccc ggcctcgggg gtcgtcctcc cgggctcggt cgcctccctg
     1501 ctggccgcag gcctggtcct cgcccggatc cagcgccgcc ggcgctggcg ccccggcgag
     1561 tcgccggccc cggtgccgga gggtccggta gccggtgagg tcgcagcctc cggggcggtg
     1621 ccgtcgctgg atctcctcgg agccctgagc gcccgggtcc tggcggcgtg gaaccaggcc
     1681 gtgggatccg tcccgaggat cctggccgcg tgggaggcgc ccggtgcagt gcggttcctg
     1741 ctctccgacg accacccgga gtccgccctg ccggagccgg tcgagctgca gggcgtcgag
     1801 gtcggcttcc accgccgcga cggggcgctg tgggccgagg caagagggga ggccgagaag
     1861 gcgctgcgcc gaaacctgct ggtcctggcc gacggcctcc tggtcccggt cgcccaccac
     1921 gccggcgggg gctgcccgcc gggcggggga gtgggcaggg cggcgctgca cctgccggtg
     1981 ctgggcaccc cgctgtgggt gagcggggcg ggggcggccg ccatggtgga gtccatcctg
     2041 atcgccgcgg ggctgcgagc cggcgccggg gacctgcggg tcgtggccgt gggcgacgtc
     2101 cccccgttgc tcgaggaggc ccgggtcccc gacgccctgg ccttcccccc gatcgagcgc
     2161 ccctccgcct ccgagctgga agccgtccgg acccgcctcg agagggccct ggtgctgcgg
     2221 gcggggcgcc tggcccaggc cgaggccgag ggcatcgagg agtacgccct ccaccac
//
LOCUS       DASWGG010000118         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2694736, whole genome shotgun sequence.
ACCESSION   DASWGG010000118 DASWGG010000000
VERSION     DASWGG010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2694736"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VF954_02090"
     CDS             <1..666
                     /locus_tag="VF954_02090"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX9529900.1"
                     /translation="TSMTPMPHLTCVAHRRDEIDDIVAGYRDEGLDNILALGGDPPAD
                     GSQPPGDFRYAVELIDAVRGIGDFCVGVAAHPELHPRSGGDRAADRRRLADKLRLADF
                     AITQFFFDPADYFSMVEEVARLGVTKPIIPGVIPVTNAGQVERFAALSGASFPPALAA
                     RLHAVADRPDEVRKIGVEVATELTAALLAGGAPGIHFYTLNFARATLEIYRNLGMGPG
                     ARA"
     gene            693..1889
                     /gene="icd"
                     /locus_tag="VF954_02095"
     CDS             693..1889
                     /gene="icd"
                     /locus_tag="VF954_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX9529901.1"
                     /translation="MAEKITMGADGTLTVPDEPIIPFIEGDGTGVDIWPASQLVFDAA
                     ATKHGRRIEWKEVLAGEKAFKETGSWLPDETVSAFKEHLIGIKGPLTTPIGGGIRSLN
                     VALRQLLDLYVCLRPVRWFAGVPSPVKHPEKVDMVIFRENTEDIYAGLEAEEGTPEAH
                     KLIAILKDTFGWDIRPDSGVGIKPISETGSKRLIRAAIQHAVAHGRKSVTMVHKGNIQ
                     KFTEGAFRNWGYELVREEFADVAVGWDDCGGEPGDKVLVRDAIADITLQQVLTRPDEF
                     EVIATTNLNGDYISDALAAQVGGIGIAPGGNINYVTGHGIFEATHGTAPKYAGQDKVN
                     PGSVILSGVMMFEHLGWTDMAADVIRALEATISDKVVTYDFARQMEGATQVKTSEFAA
                     AVVDRL"
     gene            2005..>2516
                     /locus_tag="VF954_02100"
     CDS             2005..>2516
                     /locus_tag="VF954_02100"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX9529902.1"
                     /translation="MAAPPEKRTDPVHVTVTGAAGQIGYAVLFRIASGQLLGPDQPVV
                     LRLLEIEPAMKSLEGVVMELEDCAFPLLADVVATSDLKTAFDGTSWALLVGSIPRTAG
                     MERGDLLAINGGIFQPQGQAIAAHAAGDVRVLVVGNPCNTNCLIAQRNAPEIPADRWF
                     AMTRLDQNRA"
BASE COUNT          406 a          929 c          821 g          360 t
ORIGIN      
        1 acctccatga cgccgatgcc gcatctcacc tgcgtcgccc accggcgtga cgagatcgac
       61 gacatcgtgg ccggctaccg ggacgagggt ctcgacaaca tcctggccct tggcggggac
      121 cccccggcgg acggcagcca gccgcccgga gacttccgct acgccgtcga gctgatcgac
      181 gccgtccggg gcatcggcga cttctgcgtg ggcgtggccg cccaccccga gctgcacccc
      241 cgctcgggcg gcgaccgtgc ggccgaccgg cgccggctgg ccgacaagct gcggctggcc
      301 gatttcgcca tcacgcagtt cttcttcgac ccggccgact atttctccat ggtcgaggag
      361 gtggcccggc tgggagttac caaaccgatc atcccgggcg tcatcccggt gaccaacgcc
      421 ggccaggtgg agcgcttcgc agccctgagc ggcgcctcct tccccccggc gctcgcggcg
      481 cggctgcacg ccgtggccga ccggcccgac gaggtccgca agatcggcgt cgaggtggcg
      541 accgagctga ccgccgccct gctggccggt ggggcccccg gcatccactt ctacaccctc
      601 aacttcgccc gggcgaccct tgagatttac cgcaacctcg gtatggggcc gggcgcccgg
      661 gcctgaggtc cccgacggta ggctccccgc ccatggccga gaagatcacc atgggcgccg
      721 acggcaccct caccgtcccc gacgagccca tcatcccctt catcgaaggc gacggcaccg
      781 gcgtcgacat ctggccggcc tcgcagctcg tcttcgacgc cgcggctacc aagcacggcc
      841 ggcgcatcga atggaaggag gtgctggccg gcgagaaggc cttcaaggag acggggagct
      901 ggctgcccga cgagacggtg agcgccttca aggagcacct gatcggcatc aagggccccc
      961 tcaccacccc gatcggcggc gggatccgct cgctcaacgt cgccctccgc cagctcctcg
     1021 acctgtacgt gtgcctgcgc cccgtgcgct ggttcgcggg cgtgcccagc ccggtcaagc
     1081 accccgagaa ggtcgacatg gtgatcttcc gggagaacac cgaggacatc tacgccggcc
     1141 tcgaggccga ggagggcacg ccggaggccc acaagctcat cgccatcctg aaggacacat
     1201 tcggctggga catccggccc gactcgggcg tgggcatcaa gcccatctcg gagaccggct
     1261 ccaagcggtt gatccgggcg gccatccagc acgccgtggc ccacggccgc aagagcgtga
     1321 ccatggtcca caagggcaac atccagaagt tcaccgaagg cgccttccgc aactggggct
     1381 acgagctggt ccgggaggag ttcgccgacg tcgccgtggg ctgggacgac tgcggcggcg
     1441 agcccggcga caaggtgctg gtcagggacg ccatcgccga catcacgctc cagcaggtgc
     1501 tgacccggcc cgacgagttc gaggtcatag ccaccaccaa cctcaacggg gactacatct
     1561 ccgacgccct cgccgcccag gtcgggggca tcggcatcgc gccgggcggc aacatcaact
     1621 acgtcaccgg tcacggcatc ttcgaggcga cccacggcac cgccccgaag tacgccggcc
     1681 aggacaaggt caaccccggc tcggtgatcc tgtcgggcgt catgatgttc gagcacctgg
     1741 gatggaccga catggccgcc gacgtcatcc gggccctcga ggccaccatc tccgacaagg
     1801 tggtcaccta cgacttcgcc cggcagatgg agggggcgac ccaggtcaag acctccgagt
     1861 tcgccgccgc ggtggtcgac cgcctgtagt tcgaagtcgg cacttggctg gggagataac
     1921 acaacatgag acggtgaaat ctccccagct accgccatgg gccctggttt tgcatcgggc
     1981 gcgggcggtt tctagtgttc gggcatggcc gcacctcctg agaagcgcac cgatcccgtc
     2041 cacgtgaccg tcacgggcgc ggccggccag atcggctatg ccgtcttgtt ccgcatcgcg
     2101 tcgggccagc tgctgggccc cgaccagccg gtcgtgctgc gcctcctcga gatcgagccg
     2161 gccatgaagt cgctggaggg cgtggtgatg gagctggagg actgcgcctt cccgctgctc
     2221 gccgacgtgg tggccacgtc cgacctcaag acggccttcg acggcacctc ctgggccctc
     2281 ctggtgggct cgatcccccg cacggcgggc atggagcgcg gggacctgct ggccatcaac
     2341 ggcggcatct tccagcccca gggccaggcc atcgccgccc acgccgccgg cgacgtccgg
     2401 gtcctcgtgg tgggcaaccc gtgcaacacc aactgcctga tcgcccagcg caacgccccg
     2461 gagatccccg ccgaccggtg gttcgccatg acgcgcctgg accagaaccg tgccca
//
LOCUS       DASWGG010000119         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1731183, whole genome shotgun sequence.
ACCESSION   DASWGG010000119 DASWGG010000000
VERSION     DASWGG010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731183"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1835
                     /locus_tag="VF954_02105"
     CDS             <1..>1835
                     /locus_tag="VF954_02105"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEX9529903.1"
                     /translation="LTNADTELLALRSVLHRLPDGFPPVRAANPARLDAAPELEGVRL
                     VIIRLLGGADAWRAPFEALVAGCRHADIPLVAIGGEAAPDADLAARSTVPTAIAHQVH
                     RYLAAGGPDNLAAALRFAADTLLLEGFGFDPPVEVPMTGVFEAATTERRAGRPLVGVV
                     FYRAHLIAGNTTFVEDLARALDEQGADVITVFCYSLRASAQGGVPALDILAGRGVDVV
                     VTTVLAMGAAAEDDEAGWEVPHLEALGVPVLQAPSSSRSSDEWAGDDLGLAPIDVAMG
                     VAWPEFDGRVIGPAFAFREEVDDGGDLGSVITATRADPERTARVARLAVRLGQLRHLP
                     MTDKRIAVVLSAYPTKRSRLGNAVGLDTPASTIALLHRLRAAGVAVSRIPAGGDALMA
                     ELSEHLTYETAALSPAQAARAVGRLPAGEYERWLAQLPVAARDAVEAVWGPAPGEVYV
                     ASGELCFSGLDLGGVLVAIQPPRGFGPDPIGTYHAPDLPPPHHYLAFYRWLDHVWGAH
                     AVIHVGKHGTLEWLPGKANALGPECFPDVALGSVPLVYPFVVNDPGEGTQAKRRAHAV
                     IVDHLPPPMTRADTYDDLARLEQLLDAYASAQAMDPAKLPALRAQ"
BASE COUNT          214 a          733 c          650 g          238 t
ORIGIN      
        1 tgctcaccaa cgccgacacc gagctgctcg ccctgcgctc cgtcctgcac cggctgcccg
       61 acggcttccc gccggtgcgc gccgccaacc cggcccggct ggacgcggcc cccgagctgg
      121 aaggcgtccg cttggtgatc atccggctcc tcggcggcgc ggacgcgtgg cgggccccgt
      181 tcgaggcgct cgtcgccggc tgccgccacg ccgacatacc actggtggcc atcgggggcg
      241 aggccgcgcc cgacgccgat ctggccgccc ggtccaccgt gcccaccgcc atcgcccacc
      301 aggtccaccg ctacctggcg gccggggggc ccgacaacct ggccgccgcg ctgcgcttcg
      361 cggccgacac gctgctgctg gaggggttcg gcttcgaccc gcccgtggag gtgccgatga
      421 ccggcgtctt cgaggccgct accaccgagc gccgggcggg gcggccgctg gtgggcgtgg
      481 tcttctaccg ggcccacctg atcgccggca acaccacgtt cgtggaggac ctggcccgcg
      541 ccctcgacga gcagggcgcg gacgtcatca ctgtcttctg ctactcgctg cgagcgtccg
      601 ctcagggcgg cgtccccgcc ctcgacatcc tcgccgggcg cggcgtcgac gtggtggtca
      661 ccacggtgct cgccatgggc gcggcggcgg aggacgacga agccgggtgg gaggtccccc
      721 acctcgaggc cctgggggtg cccgtcctcc aggcgccatc gagcagccgc tcatcggatg
      781 agtgggccgg cgacgacctc ggcctggccc cgatcgacgt ggccatgggg gtggcctggc
      841 cggagttcga cggacgggtg atcggcccgg cgttcgcctt ccgggaggag gtggacgacg
      901 ggggcgacct cgggtccgtc atcacagcga cccgggccga cccggagcgg accgcccggg
      961 tggcgcggct cgccgtcagg ctcggccagc tccggcacct gcccatgacc gacaagcgga
     1021 tcgcggtggt gctgtccgcc tacccgacca agcgcagccg cctcggcaac gcggtcggcc
     1081 tcgacacccc ggcgtcgacc atcgccctgc tgcaccgcct gcgggccgcc ggcgtcgcgg
     1141 tgtcccgcat cccggccggt ggcgacgccc tcatggccga gctgtccgag cacctgacgt
     1201 atgagacggc ggcgctcagc ccggcgcagg cagcccgggc ggtcgggcgc ctgccggccg
     1261 gcgagtacga gcggtggctg gcccagctgc ccgtcgcggc ccgtgacgcc gtcgaggccg
     1321 tttggggccc ggccccaggc gaggtgtacg tggccagcgg cgagctgtgc ttctccgggc
     1381 tggacctcgg gggggtgctc gtcgccatcc agccgccccg aggcttcggc cccgacccca
     1441 tcgggacgta ccacgcgccc gacctgccgc ccccccacca ctacctggcc ttctaccggt
     1501 ggctcgacca cgtgtggggc gcccacgccg tgatccacgt cggcaagcac ggcaccctgg
     1561 agtggctgcc gggcaaggcc aacgccctgg gcccggagtg cttccccgac gtggccctgg
     1621 gcagcgtccc gctcgtctac cccttcgtgg tcaacgatcc cggggagggg acccaggcca
     1681 agcgccgggc ccacgccgtc atcgtcgacc acctgccgcc cccgatgacc cgggccgaca
     1741 cctacgacga cctcgcccgt ctcgagcagc tcctggacgc ctacgcctcg gcccaggcca
     1801 tggacccggc caagctgccg gccctgcgcg cccag
//
LOCUS       DASWGG010000120         2782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1734408, whole genome shotgun sequence.
ACCESSION   DASWGG010000120 DASWGG010000000
VERSION     DASWGG010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734408"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VF954_02110"
     CDS             <1..1135
                     /locus_tag="VF954_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9529904.1"
                     /translation="HLLLCDDDGDVVRALTAGEWMVDGLDGLDEVSGVVYFSATEASP
                     LERHLYAAPLAGDVPPRRITAEQGTHQVVVDHRGEHFVDTFGALERPWTVTVRALATG
                     AIVHTVHDERDPRIEALGLVAPELFTTGAPDGTVLHGACYPPRGAIRTGGACPVIVSV
                     YGGPHAQRVTNSWSLTADLRAQLLRQEGFAVVMLDNRGSARRGLAFEGAVRHDLGRLE
                     VEDQVAGVRWAVAHLGADPGRVGIYGWSYGGYLAAMALARAPDVFVAGVAGAPVTSWD
                     GYDTHYTERYMSTPTGNPDGYRSSSVMTHAAQVRGRLLLVHGLVDENVHFRHTARLVQ
                     ALIDAGRPHELMVFPKERHVPRAEKDRAFMEARILAFFCDALS"
     gene            complement(1159..>2782)
                     /locus_tag="VF954_02115"
     CDS             complement(1159..>2782)
                     /locus_tag="VF954_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX9529905.1"
                     /translation="STAIAAFEAQVTVGGLGPWSYAGQLTLRRRAGHWLVAWSPAAIH
                     PALRSGQHLARTRTLPPRSDILAADGSKLTTTGTSVTVGLEPDHVKDRQAVLDALQQT
                     IGADPVAVGKSIDGAASHPSQFLAVAEVEEARFAAVKPTLNPIPGVLFRRHPARLAAT
                     GDLAAHVVGSVGPVTAEQVAKLGPTVLAGDLVGQSGLEAAAQRQLAGTPGGDVHVADA
                     IGATVASLQTFPGAPGQAVRTSLNLQIQKAAEHALDGVAQPAALVAIQASTGAVEASV
                     SRPVGQEFDRALDGRYPPGSTFKVITTTALLGKGITPATPVTCPPSIVAGGRKFTNFE
                     GETQPTLSFTQAFAESCNTAFIGLSSRLDAADLVGAANLYGFGLEPGLGLPAVGGRLP
                     QPADATELAADAIGQGRVDASPLAMAEVAAAVDSGAWRPPTLAPGLGPGPAPAAAPRP
                     LDPAPATSLRQLMAEVVAQGTGRAAAVPGPAVSGKTGTAEFGSAQPPQTHAWFIGFRG
                     DLAFAVLVEGGGVGGQVAAPIAARFLEGVPAG"
BASE COUNT          355 a         1013 c         1062 g          352 t
ORIGIN      
        1 ccacctcctg ctctgcgacg acgacggcga cgtggtccgc gccctcaccg ccggcgagtg
       61 gatggtcgac ggcctcgacg gcctcgacga ggtgagcgga gtcgtctact tcagcgccac
      121 cgaggccagc cccctcgagc gccacctgta cgccgcgccc ctcgcggggg acgtgccccc
      181 ccggaggatc acggccgagc agggcaccca ccaggtcgtg gtcgaccatc ggggtgagca
      241 cttcgtggac acgttcggcg cgctggagcg gccgtggacg gtcacggtgc gcgccctggc
      301 cacgggcgcg atcgtccaca ccgtccacga cgagcgggac ccccggatcg aagccctggg
      361 cctggtggcg ccagagctgt tcacgaccgg ggcgccggac gggacggtgc tgcacggcgc
      421 gtgctacccg ccacgaggcg caatccgcac cggtggggcg tgtcccgtca tcgtcagcgt
      481 ctacggcggg ccccacgcgc agcgggtcac caacagctgg tccttgaccg cagacctgcg
      541 ggcccagctg ctgcggcagg aggggttcgc cgtcgtcatg ctcgacaacc ggggcagcgc
      601 ccgccggggc ctggcgttcg agggcgccgt ccgccacgac ctcggccgcc tggaggtcga
      661 ggaccaggtc gccggcgtgc gctgggccgt cgcccacctg ggcgccgatc cggggcgggt
      721 gggcatctat ggctggagct acgggggcta cctggccgcc atggcgctgg cccgggcgcc
      781 cgacgtcttc gtcgccgggg tggccggcgc gccggtgacg tcgtgggacg gctacgacac
      841 gcattacacc gagcggtaca tgtcgacgcc taccggcaac ccggacggtt accggtcgag
      901 ctcggtcatg acccacgcgg cccaggtgcg cggccggctg ctgctcgtgc acggcctggt
      961 ggacgagaac gtccacttcc ggcacaccgc ccgcctcgtg caggccctca tcgacgcggg
     1021 tcggccgcac gagctcatgg tgttccccaa ggagcgccac gtgccccgcg ccgagaagga
     1081 ccgggccttc atggaggccc gcatcctggc cttcttctgc gacgcgctgt cctaggtagt
     1141 cgcaggcccg gccggcgctc agccggcggg cacgccctcg aggaaccggg cggcgatcgg
     1201 cgccgccacc tggccgccca ccccgccgcc ctccacgagg accgcgaagg ccagatcgcc
     1261 gcgaaagccg atgaaccagg catgggtctg gggcggctgg gcgctgccga actcggcggt
     1321 gccggtcttt ccggagacag ccggccccgg cacggccgcg gccctgcccg tgccctgggc
     1381 gaccacctcg gccatgagct ggcgcaacga ggtcgcgggc gccgggtcga gcggcctggg
     1441 ggccgccgcc ggagctggtc ctggcccgag ccccggcgcg agcgtcgggg ggcgccaggc
     1501 cccggagtcc acggccgcgg ccacctccgc catggccagc gggctggcgt cgacccggcc
     1561 ctggccgatg gcgtccgcgg ccagctcggt ggcgtcggcc ggctgcggga gccggccgcc
     1621 gacggcgggc aggcccaaac cgggttcgag gccgaacccg tagaggttgg ccgcgccgac
     1681 gaggtcggcg gcgtccaggc gcgaggacag cccgatgaag gcggtgttgc acgactcggc
     1741 gaaggcctgg gtgaagctca aggtgggctg ggtctcgccc tcgaagttgg tgaacttcct
     1801 gccgccggcc acgatcgacg gcgggcaggt gacgggggtg gctggggtga tccctttccc
     1861 caacagcgca gtggtggtga tcaccttgaa cgtcgagccc ggcgggtagc gcccgtccag
     1921 ggctcgatcg aactcctggc cgaccggacg gctgacggag gcctccaccg caccagtcga
     1981 ggcctggatc gcgaccaggg cggcgggctg ggccacgccg tcgagcgcgt gctccgccgc
     2041 cttctggatc tgcaggttga gcgaggtgcg cacggcctgg ccgggtgcgc ccggaaaggt
     2101 ctggagcgac gccacggtgg cgccgatcgc gtcggcgacg tggacgtccc cgccgggcgt
     2161 gccggccagc tgccgctggg ccgccgcctc caggcccgac tggcccacca ggtcccccgc
     2221 caggacggtt gggcccagct tggccacctg ctcggcggtg accgggccga cggacccgac
     2281 cacgtgggcc gccaggtcgc cggtcgcggc cagacgagcc gggtgccggc ggaacagcac
     2341 gccagggatc gggttgaggg tgggcttcac ggccgcgaac ctggcctcct cgacctcggc
     2401 cacggccagg aactggctgg ggtgggacgc ggcgccgtcg atgctcttgc ccacggccac
     2461 cggatcggcc ccgatcgtct gctggagggc gtcgagcacg gcctggcggt ccttgacgtg
     2521 gtcgggttcg agccccaccg tcaccgacgt ccccgtcgtg gtgagcttcg agccgtcggc
     2581 ggcgaggatg tcggagcggg gcggcagggt cctggtccgg gccaggtgct ggccgctccg
     2641 cagggcgggg tggatggcag ctggcgacca ggccaccagc cagtggccgg ccctgcggcg
     2701 caaggtcagc tgcccggcgt aggaccacgg gccgaggcca cccaccgtca cctgggcctc
     2761 gaacgcggcg atggcggtcg at
//
LOCUS       DASWGG010000121         1754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1092376, whole genome shotgun sequence.
ACCESSION   DASWGG010000121 DASWGG010000000
VERSION     DASWGG010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092376"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..549)
                     /locus_tag="VF954_02120"
     CDS             complement(145..549)
                     /locus_tag="VF954_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX9529906.1"
                     /translation="MAPPRHSSAFVDDSAMDDVAPDVAAEEDAGPAAFCPQFHQAVEL
                     IGRRWSGAIIRALLVGRTRFCEVTVAIPGLSDRLLSERLKELEAEDIVSRTVIPDTPV
                     KIEYHLTAKGAALGEVFAAISAWADQYPVPGR"
     gene            960..>1754
                     /locus_tag="VF954_02125"
     CDS             960..>1754
                     /locus_tag="VF954_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529907.1"
                     /translation="MSLAGLLGRPVRNQAGAEIGRLVDVVARLSGSELYPPVTGLVVK
                     VGRRQAFIDASAIGRLAHQDIVLNTARLDLRDFQRRAGEVMLAKDVLDHQLVDVDGVQ
                     VIRAADLYVAYALGSWRLVGVDVSLQSLVRRLGPARWRTRPTPDRVIDWAAIQPFSDI
                     GPTVKLRTSHEGLHRLRPGELADLLEDLGRPARQELLAALDPEAAADALEEMEPGELE
                     NLLREADPDQAASLVATMEPDEAVDALRDLSRDDRDEILDHMEPATA"
BASE COUNT          263 a          645 c          610 g          236 t
ORIGIN      
        1 gacgcggtcc acggcgaaga cgccccggtc cgtgcgcagc tccaccgtca ggtccggcag
       61 caacagctcc acccgcttgg gccgggagcc ggatgtgggc gccccgctga agtactggct
      121 cggctcggcg cccaccgcgt gacgctatcg acccggaacg gggtactggt ccgcccaggc
      181 cgagatggct gcgaacacct cgcccagggc agcgcccttg gcggtgaggt ggtactcgat
      241 cttcacgggt gtgtccggga tcaccgtgcg cgacacgatg tcctcggcct ccagctcctt
      301 gagccgctcg gacaggagcc ggtcggacag ccccgggatg gccacggtga cctcgcagaa
      361 acgcgtgcgt cccacgagca gcgcccggat gatggccccg gaccagcgcc ggccgatcag
      421 ctcgacggcc tggtggaact gggggcagaa cgccgccgga ccggcgtcct cctcggccgc
      481 gacgtcgggc gcgacatcgt ccatcgcgct gtcgtcgacg aaggccgacg aatgccgagg
      541 tggtgccacg cccgcagctt acccgaagga agcagcttgt cgcccgcgcg ggggacgatc
      601 agggaagctg cttatggaac ataaggaata aggcggggcc cttgctggac cgcggcgcta
      661 gcatggtgaa gtccggcaac cagaaggagg tgaggcgcgt gaaacctggt gaacccccga
      721 gtaccacgtc acccctgccc gcctcctcct tcgccgccgc cctcgtggcc tagccactcg
      781 cctcgcgcgg ctgccgccat aggcggagat gtggggtcca gcccgcttcc acctgcgctt
      841 gcgtgaggga gaacgccatg gcccggcgcc cgactttgcc cacttcgaac ctccggccca
      901 gccggcgtat ccaacggacc cgccggctac taaccacgcg cgccgtcaag gaagccgtcg
      961 tgtccctcgc cggcctgctc gggcgcccgg tccgcaacca ggccggcgcc gagatcggcc
     1021 ggctggtgga cgtggtggcc cgcctgtcgg gctccgagct gtaccccccc gtcaccgggc
     1081 tggtcgtcaa ggtgggccgg cggcaggcct tcatcgacgc gtccgccatc gggcgcctgg
     1141 cccaccagga catagtgctg aacaccgctc ggctcgacct ccgggacttc cagcgccggg
     1201 cgggcgaggt catgctggcc aaggacgtcc tcgaccacca gctggtcgac gtcgacgggg
     1261 tccaggtgat ccgggccgcc gacctgtacg tggcgtacgc cctgggatcg tggcggctgg
     1321 tcggtgtcga cgtgagcctc cagtccctcg tgcgccggct gggcccggcc cggtggcgta
     1381 cccggcccac gccggaccgg gtgatcgact gggcagccat ccagcccttc agcgacatcg
     1441 ggccaaccgt gaagctgcgc acctcccacg agggcctgca ccggctgcgt cccggtgagc
     1501 tggccgatct gctcgaggac ctcggccgcc ccgcccgcca ggagctgctg gccgccctgg
     1561 acccggaggc ggcggccgac gccctggagg agatggagcc gggcgagctg gagaacctgc
     1621 tgcgagaggc cgaccccgac caggccgcca gcctggtggc caccatggag cccgacgaag
     1681 ccgtcgacgc cctgcgggac ctgtccaggg acgaccgcga cgagatcctc gatcacatgg
     1741 agccggccac ggct
//
LOCUS       DASWGG010000122         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_767461, whole genome shotgun sequence.
ACCESSION   DASWGG010000122 DASWGG010000000
VERSION     DASWGG010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767461"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VF954_02130"
     CDS             <1..789
                     /locus_tag="VF954_02130"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HEX9529908.1"
                     /translation="PRDVLELPVMAAATRAVAGGRVRLQALVAEHPERETRRVIVYRR
                     EAGAYRGREAVVAPADVPAVVEALAGGGGEETTRRDVLVCTHGRRDACCGSRGTELFA
                     AAAALSPDPRLRWWRTSHTGGHRFAPTAVLLPEGQSWAYLDPPLLAGVLARAGEVPGR
                     HFRGSAALPTPAVQAVEREAFVAQGWDWLSFARRGWEESPAGAAVTAVVEYRSPAGVH
                     GTYRGRVRMARVLPVPDCGRPVEDATKSEPELRVDSLAHERAED"
     gene            complement(786..1208)
                     /locus_tag="VF954_02135"
     CDS             complement(786..1208)
                     /locus_tag="VF954_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX9529909.1"
                     /translation="MTLKAIDVEVGHTHSQVLVTDLKRTQIVQYAGTSGDYNPLHTDE
                     IFATKVAGYPSVFAHGMLTMGMTGRLVTDYVGDGQLTRFGGRFTSQVFPGDTLTGTLT
                     VEATRDEDGGKVVDLKVSTVNQDGVEVFAGTATARVES"
     gene            complement(1214..1690)
                     /locus_tag="VF954_02140"
     CDS             complement(1214..1690)
                     /locus_tag="VF954_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9529910.1"
                     /translation="MAVDRFPIEYGHILMFARSIGDPNPIYEDPERAIAPPTFVQASA
                     QFDPDYFLRPKIGRPWMGSGKEPTGVTREGGGGGGGGGGGLHAEQHFEYHRHLKPGDV
                     LSATTTPGRTWEKEGRRAGKLVFNEGVTEYRDQNGDLVVTAKVVGVRTERPATAGA"
     gene            complement(1915..>2534)
                     /gene="glnII"
                     /locus_tag="VF954_02145"
     CDS             complement(1915..>2534)
                     /gene="glnII"
                     /locus_tag="VF954_02145"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX9529911.1"
                     /translation="GPQGPYYCGVGADDIFGRDLAEAHLDACLRAGLDISGINAEVMP
                     GQWEFQVGPVGPLEVSDQLWVARWLLHRLGEDLEISVTLDPKPVRGDWNGAGAHTNFS
                     TQAMRSSYDAVITAAEALGKNADAHVPHYGAGIEHRLTGQHETAPWDEFSYGVSDRGA
                     SVRIPWQVEKDQRGYIEDRRPNANVDPYVVTRLIVDTCCSALATP"
BASE COUNT          306 a          916 c          931 g          381 t
ORIGIN      
        1 ccgcgcgacg tcctcgagct gcccgtaatg gcggccgcga cccgggccgt tgccggcggt
       61 cgggtccggc tccaggccct ggtcgccgag caccccgagc gcgagacccg ccgcgtgatc
      121 gtctaccgac gggaagcggg cgcgtaccgc ggccgggagg ccgtcgtcgc cccggccgac
      181 gtgcccgccg ttgtggaggc cctcgccggc ggcgggggcg aagagaccac ccgccgcgac
      241 gtgctcgtct gcacccacgg ccggcgggac gcgtgctgcg ggagcagggg cacggagctg
      301 ttcgccgccg cggccgccct ttctcccgat ccccggctgc gatggtggcg caccagccac
      361 accggcggcc accgcttcgc cccgacggcc gtcctgctgc ccgaaggcca gtcgtgggcc
      421 tacctcgacc cgccgctgct ggccggggtc ctcgctcggg ccggcgaggt cccaggccgc
      481 cacttccggg gcagcgcggc gctgccgacg cccgctgtgc aagcggtcga gcgggaggcc
      541 ttcgtggccc agggttggga ctggctttcc ttcgcccgcc ggggatggga ggaaagcccg
      601 gccggcgccg ccgtgaccgc cgtggtcgag taccgctccc ccgccggcgt acacggcacc
      661 taccgcggcc gggtcaggat ggcgcgggtg ctgcccgtgc ccgattgcgg ccggcccgtc
      721 gaggatgcca ccaagagcga gccggagctg cgcgtggact cactggccca cgagcgagct
      781 gaggactagg actccacccg ggccgtggcc gtcccggcga acacctcgac accgtcctgg
      841 ttgaccgtgc tcaccttgag gtcgaccacc ttgccgccgt cctcgtcgcg ggtggcctcg
      901 accgtcagcg tcccggtgag ggtgtcaccg gggaacacct gcgaggtgaa ccggccgccg
      961 aacctggtga gctgcccgtc cccgacgtaa tcggtcacga gcctccccgt catgcccatg
     1021 gtcagcatgc cgtgggcgaa gaccgacggg tagcccgcca ccttggtggc gaagatctcg
     1081 tcggtgtgca gcgggttgta gtcgccggaa gtgcctgcgt actggacgat ctgcgtgcgc
     1141 ttgaggtcgg tgaccagaac ctggctgtgg gtgtggccca cctcgacgtc gatggctttg
     1201 agggtcatgt ccgtcacgct cctgccgtcg ccggccgctc ggtccgcaca cctacgacct
     1261 tcgccgtcac caccaggtcg ccgttctggt cgcggtactc cgtgacccct tcgttgaaca
     1321 ccagcttgcc ggcccggcgc ccttccttct cccacgtcct gcccggcgtg gtggtggcgc
     1381 tcagcacgtc gcccggcttc aggtggcggt ggtactcgaa gtgctgctcg gcgtgcagcc
     1441 cgccgccgcc gccgccaccg ccaccgccgc cctcgcgtgt gacaccggtc ggctccttgc
     1501 ccgatcccat ccagggccgg ccgatcttgg gccgcaggaa gtagtcgggg tcgaactggg
     1561 cgctggcctg cacgaaggtc ggcggggcga tcgctcgttc ggggtcctcg tagatcgggt
     1621 tcgggtcccc gatgctgcgg gcgaacatca agatgtgccc gtactcgata ggaaagcggt
     1681 caaccgccat gtcggtcccc ctaagaaggt cggtcggcgg gagcctatcc gccgcggcct
     1741 tccccccgcc cgggtcccgc ccgggcctat gagcacggcc cggcgtgggg ttggggcccc
     1801 acgctcggga ggagggcggg gttggggccc cgcccgggcc tacggcagca cggcccggcg
     1861 tggggttggg gccccacgct cgggaggagg gcggggttgg ggccccgccc gggcctacgg
     1921 cgtcgcgagg gccgagcagc aggtgtccac gatgagccgg gtcacgacgt agggatcgac
     1981 gttggcgttg ggccggcggt cctcgatgta gcccctctgg tccttctcca cctgccacgg
     2041 gatgcggacc gaggctccac ggtcggacac gccgtagctg aactcgtccc acggggccgt
     2101 ctcgtgctgg ccggtcaggc ggtgctcgat cccggcgccg tagtggggca cgtgggcatc
     2161 ggcgttcttg ccgagcgcct cggccgcggt gatcacggcg tcgtacgagc tgcgcatggc
     2221 ctgggtggag aagttggtgt gggcgccggc gccgttccag tcgccgcgga ccggcttggg
     2281 gtcgagcgtg acggagatct ccaggtcctc gccgaggcgg tgcagcagcc agcgggccac
     2341 ccacagctgg tccgacacct cgagcgggcc gaccgggccg acctggaact cccactgccc
     2401 gggcatgacc tcggcgttga tgccggagat gtcgagcccg gcccggaggc aggcgtcgag
     2461 gtgggcttcg gccaggtccc gaccgaagat gtcgtccgcg ccgaccccgc agtagtaggg
     2521 gccctgcgga cccg
//
LOCUS       DASWGG010000123         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1737319, whole genome shotgun sequence.
ACCESSION   DASWGG010000123 DASWGG010000000
VERSION     DASWGG010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737319"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VF954_02150"
     CDS             <1..830
                     /locus_tag="VF954_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9529912.1"
                     /translation="LKIPSVGGDSLNYDWSQSPVLFPQGATFSATIIGAVKQLADQGK
                     TKLGVIYCVEARNCQDANKVMVDGGDKVAGATVVYRSQISLAQTGFGGQCQAAKDAGA
                     QQVLLAMDGSAISRVMKSCAQIDYFPAIATSPIAIGSSVTQDPNVRKAGVSLAHIAFP
                     WTQADNPAEQRYQQAMARSAPGVASDGATSQAWVAGEMLRTLVDSLGDAGLGDITTEL
                     VYRGLGNLKGTTLDGLTSPLTFSAGQSKAPENNCYFPVLLTEDKGFVAPNGSRPTCL"
     gene            865..1488
                     /locus_tag="VF954_02155"
     CDS             865..1488
                     /locus_tag="VF954_02155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9529913.1"
                     /translation="MKLLLVRHSEPDPGRDVLDPPLTENGRRLAAATGEWLAGEGIAC
                     VYSSSSRRAVETAELIAGSVDAPVVQREGLVEFGDGQEYVPVEELRRTGDARWTAMAA
                     GDLAVFGTDAETFRAEVSAAVGAIADAHPGQTVAVVAHAGVINAYLGDMLDIDRLLWV
                     ELDYAAICRVAVSRSGVRSVLALNERPHACGLPSDSLLTTPLPGGSS"
BASE COUNT          242 a          590 c          478 g          206 t
ORIGIN      
        1 agctcaagat ccccagcgtg ggcggcgact cgctcaacta cgactggtcg cagagcccgg
       61 tcctgttccc ccagggcgcc acgttctcgg ccaccatcat cggtgccgtg aagcagctgg
      121 ccgaccaggg caagaccaag ctcggcgtca tctactgcgt cgaggcccgc aactgccagg
      181 acgccaacaa ggtcatggtc gacggcggcg acaaggtggc cggcgccacc gtcgtgtacc
      241 gcagccagat ctcgctcgcc cagaccggct tcggcggcca gtgccaggcg gccaaggacg
      301 ccggcgccca gcaggtcctc ctggccatgg acggctccgc catcagccgg gtcatgaagt
      361 cctgcgccca gatcgactac ttcccggcca tcgccacctc acccatcgcc atcggctcct
      421 cggtgacgca ggaccccaac gtccgcaagg ccggcgtctc cctggcccac atcgccttcc
      481 cctggacgca ggccgacaac ccggccgagc agcgctacca gcaggccatg gcccgctccg
      541 cccccggtgt ggcgtccgac ggcgccacct cgcaggcgtg ggtggccggc gagatgctcc
      601 gcaccctcgt cgacagcctc ggcgacgccg gcctcggcga catcaccacc gagctcgtct
      661 atcgcggcct cggcaacctc aagggcacga cgctcgacgg cctgacctcg cccctcacct
      721 tctcggccgg ccagtcgaag gcgcccgaga acaactgcta cttccccgtc cttctgaccg
      781 aggacaaggg gttcgtggcc ccgaacggaa gccggcccac gtgcctgtga agccggagcc
      841 aacccactca gaaagagacc gaccgtgaaa ctgctcctcg tccgccacag cgaaccggat
      901 cccgggcgcg acgtcctcga cccgccgctc accgagaacg gccggcggct ggcggccgcc
      961 acgggggagt ggctcgccgg tgaggggatc gcatgcgtct actcgagctc gagccgacgg
     1021 gcagtcgaga ccgccgagct gatcgccggg tcggtcgacg cccccgtcgt gcagagggaa
     1081 ggactcgtcg agttcggcga cggccaggag tacgtaccgg tcgaagagct gcgccggacc
     1141 ggcgacgccc gctggacggc catggccgcc ggtgacctcg ccgtcttcgg caccgacgcc
     1201 gagaccttcc gggccgaggt gtcggcggcc gtgggggcca tcgccgacgc ccatccgggc
     1261 cagacggtgg cggtggtcgc ccatgccggc gtcatcaacg cctacctcgg cgacatgctc
     1321 gacatcgacc gcctcctgtg ggtcgagctc gactacgccg ccatctgccg cgtggccgtc
     1381 agccggagcg gggtccgcag cgtcctcgcc ctcaacgaac ggccccacgc ctgcggcctt
     1441 ccgtccgatt ccctgctcac cacgcccttg ccaggaggtt cctcgtgaac gactggatcc
     1501 tgaacgacac cccttc
//
LOCUS       DASWGG010000124         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2703825, whole genome shotgun sequence.
ACCESSION   DASWGG010000124 DASWGG010000000
VERSION     DASWGG010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2703825"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..873
                     /gene="hcaB"
                     /locus_tag="VF954_02160"
     CDS             55..873
                     /gene="hcaB"
                     /locus_tag="VF954_02160"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: protein motif:HMM:NF004849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(cis-5,6-dihydroxycyclohexa-1,
                     3-dien-1-yl)propanoate dehydrogenase"
                     /protein_id="HEX9529914.1"
                     /translation="MGWLEGQAALVTGGASGLGRAVVDRFVQEGARVVVLDRSAEHLE
                     KLRADHDRRTVVAVKGDVTSPAANEKAVAAAVKAFGGLDVFVGNAGIWDYSIPLAHLP
                     LNMLGEVFDEVFGVNVKGYILGTKAALPELVKSRGCVIYTLSNAAFDPAGGGVFYTAS
                     KHAVRGLVTELAYELAPKVRVNGVAPGGCGTDLRGPKCLGFDRVSLGGVPGIDDLVKA
                     VTPLGFLPAAEDYAGLYVLLASRENSRTVTGSILRGDGGIGVRGLTQARGGDDL"
     gene            complement(870..1640)
                     /locus_tag="VF954_02165"
     CDS             complement(870..1640)
                     /locus_tag="VF954_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9529915.1"
                     /translation="MDTTTAAGKLLALLDAFGPEQPDFSLAELAVRSGLPKSTAHRVL
                     AVLESWRGVERSESGRYRLGIKFFELGGLVQDRLRLREVALPYMEDLLATTREMVHLA
                     KLDGNDVLYIERLVSHRSVRSPSRVAGRVPATCTGVGKAILAFSPPDVVRSVIAAGLP
                     ALTAYSITNPVQFLETLERIRTTGVSFDSEEASLGLNCVAAPIFNHTGAPVAAMSVSG
                     PVGRLSPEALVPAIRAATLAVGRHLGYQPRSKTTPAAS"
BASE COUNT          276 a          679 c          708 g          267 t
ORIGIN      
        1 acctcagtgg caacagcatg aagtttcatt actcgggaac tggaggcaaa cggcatggga
       61 tggctggagg gacaggcagc gctggtgacc ggcggagcgt ccgggttggg gcgggcggtg
      121 gtcgaccgct tcgtgcagga gggggcccgg gtcgtcgtcc tcgaccgctc ggccgagcac
      181 ctcgagaagc tgcgggccga ccacgaccgc cgcacggtcg tggcggtgaa gggcgacgtg
      241 accagcccgg cggccaacga gaaggcggtg gccgccgccg tcaaggcctt cggcgggctc
      301 gacgtcttcg tcggcaacgc cgggatctgg gactactcga tccccctcgc ccacctcccg
      361 ctcaacatgc tgggcgaggt gttcgatgag gtcttcggcg tcaacgtgaa gggctacatc
      421 ctcggcacca aggcggcgct gccggagctg gtcaagagcc ggggctgcgt gatctacacc
      481 ctgtcgaacg ccgccttcga ccccgccggc ggcggcgtct tctacaccgc ctccaagcac
      541 gccgtgcggg gcctcgtcac cgagctggcc tatgagctgg cccccaaggt gcgggtcaac
      601 ggcgtcgctc ccggcgggtg cggcaccgac ctgcggggcc cgaagtgcct gggcttcgac
      661 cgggtgtcgc tgggcggcgt gccgggcatc gacgacctcg tgaaggccgt cacgcccctg
      721 gggttcctgc cggcggccga ggactacgcc ggcctctacg tcctcctcgc ctcccgggag
      781 aacagccgga cggtcaccgg ctcgatcctc cggggcgacg gcggcatcgg cgtccggggc
      841 ctgacccagg cccggggcgg agacgacctt taggacgcgg ccggggtcgt cttggaccgg
      901 ggctggtagc cgagatggcg gccgacggcc agggtggcgg cccggatggc cggcaccagc
      961 gcctcggggc tcagccgccc gaccgggccc gacaccgaca tggccgccac cggcgccccg
     1021 gtgtggttga agatcggggc ggccacgcag ttcagcccca ggctcgcttc ctcgctgtcg
     1081 aacgacaccc cggtggtgcg gatgcgctcc agcgtctcga ggaactgcac cgggttggtg
     1141 atgctgtagg cggtgagcgc cggcaggccg gcggcgatga ccgaccgcac cacgtcgggc
     1201 gggctgaagg ccaggatggc cttccccacg ccggtgcagg tggccggcac ccggcccgcc
     1261 acccgggacg gcgaccgcac gctgcggtga ctcaccagcc gctcgatgta gaggacgtcg
     1321 ttgccgtcga gcttggccag gtggaccatc tcccgggtgg tggccagcag gtcctccatg
     1381 tagggcaggg ccacctcccg gagccgcagc cggtcctgca ccagcccgcc gagctcgaag
     1441 aacttgatgc cgaggcggta ccggcccgac tcgctgcgct ccaccccccg ccacgactcc
     1501 agcacggcca ggacccggtg cgccgtcgac ttgggcagcc ccgaccgcac ggccagctcg
     1561 gccaggctga agtcgggctg ctcgggcccg aacgcgtcga gcagcgccag cagcttcccg
     1621 gccgcggtgg tcgtgtccat gctctggccg gaagcggcgt tccgtccaga ggaacgagtc
     1681 ggtggcacgc tctccccttt gcgccccaaa ctgtcccctg cagcgggccg gaacctatca
     1741 gtgatagcga tcgttcacca gaggcgttcc atctgctgga acgcccggga ggccggcccg
     1801 tcacgacgtc gggaccacag ggggagcgca tggccgaggc ggaagccatc cattcggtgg
     1861 ccaaggagtt gcggacggcg gaggagcagc ggtcgccggt gccgccggtg gcggcgcgtt
     1921 tcggcgccct
//
LOCUS       DASWGG010000125         3176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2318080, whole genome shotgun sequence.
ACCESSION   DASWGG010000125 DASWGG010000000
VERSION     DASWGG010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2318080"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VF954_02170"
     CDS             complement(<1..1001)
                     /locus_tag="VF954_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX9529916.1"
                     /translation="MRRYLALTAAATTSMVVLAFTVPLAVLVRDKAGQQAVARASLEA
                     QSLVSVLTAVRDPQSRAQVVERLRQRTADDITLYLPDGTTVGVPAAVDAGVTEAMQGK
                     AFTRARQGGRDVLVPVVADNGQTMVVRVGVPASKLRRGLARAYALLALVALSLLGIAI
                     VVADRLARFFVRPIDDLAATARTLEAGDLQARAAVAKPVEVAEVGNALNRLAQRILDM
                     IAAEREALADMSHRLRTPLTALRLEAESITIAEEAARFGASVDELAAALDDLIAEARR
                     PSAGPVELVDLADVVAERARFWSALADEQERPFVLHVDPGELHIAVSRTQLVAAVDA"
     gene            complement(998..1726)
                     /locus_tag="VF954_02175"
     CDS             complement(998..1726)
                     /locus_tag="VF954_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9529917.1"
                     /translation="MALVALIEDDPHIQRALARALEHRGHVVQGFLRPMPALAELTAR
                     PADVVILDLALPDIDGMELLKMLRAVHDTPVIVASARDGEGGIVEALDAGADDYVVKP
                     FSAEQLDARIRAVLRRSSMVRGAPPLVVGGLVVDCRSRQATLDGRTLDLNRKEFDILA
                     YLASRQDEVVSKRDLWRDVWHEVGRGTDKTIDVHLSWLRRKLGESSSAPRYLHTVRGV
                     GVRVVAPDPGGLVPVEAEPISPES"
     gene            2185..2580
                     /locus_tag="VF954_02180"
     CDS             2185..2580
                     /locus_tag="VF954_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529918.1"
                     /translation="MQSTGSAVPGVRTSSQATSTTGPGTGASSPQSTTTTPPAGPSGT
                     TTTTVPTTTTTTTSQVFSYTMRGGSVGIRCNVNQVSLAYATPDNGYSVEVDSSGPNEV
                     AVRFIQSSSTTTFRSTCQDGQPQPSITSS"
     gene            2623..>3176
                     /locus_tag="VF954_02185"
     CDS             2623..>3176
                     /locus_tag="VF954_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9529919.1"
                     /translation="MTSVIDDTTISSDEKLVRERVAELLGTHDPATTELKDFWGAQYD
                     LGLAWVHFPEGSGGLGVSPKLQEVVAEGMRRAGAPVGLNYGRNPIGYGMGAPTVVTHG
                     SEAQKRRYLRPLFTTEEIWCQLFSEPGAGSDVASLSTRAVRDGDEWTVNGQKVWTTLA
                     HVARWGMLVARTDPDQPKHKGLTY"
BASE COUNT          474 a         1175 c         1123 g          404 t
ORIGIN      
        1 aaggcatcga cggcggcgac cagctgcgtg cgggacacgg cgatatggag ctcgcccggg
       61 tcgacgtgca gcacgaaggg ccgctcctgc tcgtcggcaa gggccgacca gaagcgagcg
      121 cgctcggcca cgacatcggc caggtcgacc agctcgacgg gaccggcgga ggggcgccgg
      181 gcctcggcga tgagatcgtc gagcgcggca gccagctcgt cgacggacgc tccgaaccgg
      241 gccgcctcct ccgcgatcgt gatgctctcg gcctccagcc ggagcgcggt gagaggcgtc
      301 cgcagccggt gggacatgtc ggccaaggcc tcccgctcgg ccgcgatcat gtcgaggatc
      361 cgctgggcca gccggttcag cgcgttgccc acctcggcca cctcgacggg cttggcgacc
      421 gcggcgcggg cttgcaggtc gcccgcctcg agcgtgcgcg cggtggccgc caggtcgtcg
      481 atgggccgca cgaagaaccg ggccagccgg tcggccacga cgatggcgat gcctaggagc
      541 gacaaagcca ccagggccaa gagggcgtaa gcccgggcga gaccccgtcg cagcttgctc
      601 gccggcacgc ccacccggac gaccatcgtc tgcccgttgt cggcgacgac gggcaccagc
      661 acgtcccgcc ccccctgccg ggcccgggtg aaggctttcc cctgcatcgc ctccgtcacg
      721 cccgcatcga cggctgccgg cacgccgacc gtcgtgccgt caggcaggta cagggtgatg
      781 tcgtcggcgg ttcgctgccg cagccgttcc acgacctggg cgcgcgactg cgggtcgcga
      841 acggccgtca gcaccgagac cagcgactgc gcctcgaggc tggcgcgcgc caccgcctgc
      901 tggccggcct tgtcgcggac gaggacagcc aacgggaccg tgaaggccag cacgaccatg
      961 gaggtggtcg ccgccgccgt cagcgcgagg tatcgcctca cgactccgga gagatcggct
     1021 ccgcctcgac cggcaccagt ccgcccgggt cgggggccac gacacgaacc ccgacaccac
     1081 gcacggtgtg cagatagcgg ggcgccgagg acgactctcc gagcttgcgc cggagccagg
     1141 agaggtggac gtcgatggtc ttgtcggtgc cccgcccgac ctcgtgccaa acatcgcgcc
     1201 acaggtcccg tttgctcacg acctcgtcct gacgggatgc caggtaggcg aggatgtcga
     1261 actccttgcg gttcaggtcg agggtgcgcc cgtcgagcgt ggcctggcgg gaccggcagt
     1321 ccacgaccag cccaccgacc acgagcgggg gggctccgcg gaccatcgag ctccggcgca
     1381 gcacggcccg gattcgggcg tcgagctgct cggcggagaa gggcttgacg acgtagtcgt
     1441 cggcgccggc gtcgagcgcc tcgacgatgc ccccctcgcc gtcgcgggcg ctggccacga
     1501 tgaccggggt gtcgtgcacg gcccggagca tcttgagcag ctccatgccg tcgatgtccg
     1561 gaagggcgag atccaggatg acgacgtcgg cgggccgggc ggtcagctcc gccagcgccg
     1621 gcatcggccg gaggaacccc tgcacgacgt gaccacggtg ctcgagcgcc cgggccagcg
     1681 cccgctggat gtgcggatcg tcctcgatca acgccacgag cgccatcccg ccacgatagt
     1741 caccggggcc gacggccctg ccggcggccg gcgccgtctc gtccggtcgg ctcgtgcggc
     1801 cagatttggg cgtcccttag cgttctctta ggtgggccct ggggacaacc ctccaacatg
     1861 tcgcgccgcg cgttggtcct cgggctctgg ttgtcggcaa ccggcggcgc caccggcctg
     1921 gtggccacag cggtgcgcat ggcggtcgtc ggcgtggcgt cgccgggcgc gccctccgac
     1981 ccggccaacg ggggtttcgg gccatcgcgg gacgggcgcg gacccgtcct ggccgcagct
     2041 gcgaccggcg agcgcgggcc ccgcccctca ccgacggcag cctcgcccgc cggtccgccg
     2101 ggcggtgcga acgggaccgg ttctccgaac cgcccgaacg gcaaccatgc cgcggtcgcg
     2161 gtggcgggcg cccgtcctcc ggcgatgcag tcgaccggct cggccgtccc gggggtgcgg
     2221 acgagctccc aggcgacatc gaccaccgga ccgggcaccg gagcatcgag cccgcagtcg
     2281 acgaccacga ccccgcccgc cgggccctcc ggcaccacca cgaccaccgt ccccaccacc
     2341 accacgacca cgacgtcgca ggtcttctcc tacaccatgc ggggcgggag cgtgggcatc
     2401 cgctgcaacg tcaaccaggt gtccctggcc tacgccacgc ccgacaacgg ctattcggtc
     2461 gaggtcgaca gctcagggcc caacgaggtc gccgtccgct tcatccagag ctcctccacg
     2521 accacgttcc gctccacgtg ccaggacggc cagccccagc cctccatcac cagcagctga
     2581 gcccatgacg cctggcgtgc tcccaaagta tcgtggctcc ccatgaccag cgtgatcgac
     2641 gacacaacca tctcgagcga cgagaagctc gtgcgcgagc gggtggccga gcttttgggg
     2701 acccacgatc cggccacgac cgagctcaag gacttctggg gggcgcagta cgacctcggg
     2761 ctggcgtggg tccacttccc ggagggctcc ggcgggctgg gtgtgagccc caagctccag
     2821 gaggtggtcg ccgaaggcat gcggcgggcg ggcgcgcccg tcgggctgaa ctacgggcgc
     2881 aatcccatcg ggtacggcat gggcgccccg acggtggtga cccacggcag cgaggcccag
     2941 aagaggcgct acctccgccc cctgttcacg accgaggaga tctggtgcca gctgttcagc
     3001 gagcccgggg cgggctccga cgtggccagc ctctcgaccc gggccgtgcg cgacggcgac
     3061 gagtggaccg tgaacggcca gaaggtgtgg accaccctgg cccacgtggc gcgttggggg
     3121 atgctcgtgg cccgcaccga cccggaccaa cccaagcaca agggcctgac ctactt
//
LOCUS       DASWGG010000126         3399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2705566, whole genome shotgun sequence.
ACCESSION   DASWGG010000126 DASWGG010000000
VERSION     DASWGG010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2705566"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2413
                     /locus_tag="VF954_02190"
     CDS             <1..2413
                     /locus_tag="VF954_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEX9529920.1"
                     /translation="ALRCLEAELKHRERALRQARASDLAEYRRLASDGIDHGSNNQGR
                     NNQGRNNQGGIGQGWMGEGPLQPLPRLVIVIDEFATLATELPEFIDALIGVAQRGRSL
                     GVHLILATQRPSGAVSDNIRANTNIRIALRVQDAAESCDVVGTPDAAALDRRTPGRAV
                     LRLGPSELVVFQAARVSQPTPGPRGEPALRLEPFRLFDGSPGPPWLSPVDDPADPAAE
                     AAGVPPVPRPSEPSDLARIVAAARSAFALRGGRPPRAPWPTPLPPILDRADVVDGRDG
                     ADGISAAEAVILGLADEPDAQRQEPYRWDPSVTNLLVVGPAGSGTTSAIVTAALALAT
                     RRRPDQLHLYVMDFGSQSLAPLARLPHCGGVVAAGDAERQERLIRMLGDSMDRRRGTR
                     PPDGAWPRLVVLIDNYGGLHHAWDDVARLQWRDDLARIVADGPGLGISVVISADRPGS
                     VPVSVSSSIVQKLLFRTADPYDLAAFGISPNRGPAAIPGRAVDAATGRHVQLVRATPT
                     GLDGLAAAVEALRAKAPAGPGTAPGESCRGAASGHVDEPGQVPAAGPACGQAGKTVPE
                     GAAGRMGAQPLPIHRLPSRVAHQEVGTGRLLSSGELILPVGIGDRHLAPACVRLGDGE
                     HLLVAGPARSGKSSVLVHLASKAAEAGLMVSAAAPRRSPLRSAGLAHVATEAGDIVSL
                     FESSFEQAAGPDRRRLLLVDDAELVDDPSGDIDRILSARRPGLHVVAAGRSDVLRTAF
                     GHWTAEVRRSRQGLVLRPQPELDGELWHVSLPRRSPTQWTAGRGYLVIDGEVELVQAV
                     ETRPA"
     gene            2410..>3399
                     /locus_tag="VF954_02195"
     CDS             2410..>3399
                     /locus_tag="VF954_02195"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9529921.1"
                     /translation="MINERYELDRPIGRGSMGVVYRATRLAAPGGVVAIKRVPVASDP
                     MLRERLRAEAEVLALLDHPHIVRVHEVIDDGDGLAVVMPLATGGSLADRLVREGRMDA
                     TVVASFAAEIGDALSCAHRQGVLHCDVKPSNILFTSEGDALLTDFGLARWVSRPSVWG
                     GAVMGTAEYLDPEVADGLQPDSRSDVYSLAVVCYEALTGRVPFTGPTALAVLKRAERG
                     RATAVANLVPDLPVGLSEAVAEGMARKRSRRPESAQALAAALRSSGTLAQPRARPCEA
                     GPGTSAPPAAEMGCSSGPPTARFDFRPLGPHDPGEEPRADARARRSPARPALVA"
BASE COUNT          447 a         1229 c         1237 g          486 t
ORIGIN      
        1 agccctgcgg tgcctcgagg ccgagctcaa gcaccgcgag cgcgccctgc gccaggcgag
       61 agccagcgat ctcgccgagt accggcggct ggccagtgat gggatcgacc acggcagcaa
      121 caaccagggc aggaacaacc agggcaggaa caaccagggc gggatcggcc agggatggat
      181 gggggaaggt ccgctccaac cgttgccgcg cctggtcatc gtcatcgacg agttcgccac
      241 cctcgccacc gagcttcctg agttcatcga cgccctcatc ggcgtggcgc aacggggccg
      301 gagtctcggg gtgcacctga tcctcgcgac gcaacgccca tccggtgccg tcagtgacaa
      361 catccgggcc aacaccaaca tccggatagc gctccgcgtg caggacgccg cggaatcatg
      421 tgacgtggtt ggcacacctg atgccgcggc cctcgatcga cgcacgccag gccgcgccgt
      481 gctgcgcctt gggccttcgg agctggtggt cttccaggcg gccagggtga gccagcccac
      541 gccgggacca cggggtgagc ccgcgcttcg gctggagccg ttccgcctgt tcgacggctc
      601 gcccgggccg ccttggctgt cgcctgtcga cgatcccgcg gacccggcag ctgaggcggc
      661 cggggtgcca cccgtacccc ggccgagcga gcccagtgat cttgctcgca tcgtcgcggc
      721 ggcccggtcg gccttcgcgc tccgtggcgg ccgaccgcct cgcgccccct ggccaacacc
      781 cttgcctccg atcctcgacc gggcggatgt cgtcgatggc cgagacggcg ccgacggaat
      841 ctccgccgcg gaagccgtca tcctcgggct cgcggacgaa cccgacgccc agcgccaaga
      901 gccgtaccga tgggacccgt cggtcaccaa cctgctggtg gtcggcccgg cgggctcggg
      961 tacgacgagc gccatcgtga ctgccgccct ggcgctggcg acgcgccggc ggccggatca
     1021 gctgcatctc tacgtcatgg acttcggcag tcaatcgctg gctccgctgg cccgcctgcc
     1081 ccattgtggt ggcgtggtgg cggcgggcga cgccgagcgc caagagcgcc tgatccggat
     1141 gctgggcgac agcatggacc gccggcgggg cacgagaccg ccggacggcg cctggccccg
     1201 gctggtggtt ctgatcgaca actatggcgg gctgcaccac gcgtgggatg acgtggcccg
     1261 actgcagtgg cgcgacgacc tggccaggat cgtcgccgac ggtccggggc tcggcatctc
     1321 ggtcgtcatt tcagcggacc ggcccgggtc cgtgccggtg tcggtcagct cgtcgatcgt
     1381 ccaaaagctg ctcttccgca cggccgatcc gtacgatctc gccgcgttcg gcatctcccc
     1441 gaatcgcgga ccggccgcca tcccgggccg cgctgtggac gcggcgacgg gtcggcacgt
     1501 gcagctcgtc cgggccacgc cgacggggct ggacggactg gccgccgcgg tggaagcgct
     1561 cagagccaaa gccccagcgg gcccgggcac ggccccgggt gaaagctgcc ggggcgcggc
     1621 atcgggccac gtggacgagc cgggccaggt cccggcggcg ggtccggcgt gcggccaggc
     1681 cgggaagacg gttccagagg gcgcggccgg aaggatgggc gcccagcccc tgcccataca
     1741 ccgacttccg agccgggttg cccaccagga ggtgggaacc ggtcgactgc tgtcgagcgg
     1801 cgagctcatc cttccggtgg gcatcgggga ccgccacctc gctccggcct gcgtccggct
     1861 gggtgacggc gagcacctcc tcgttgccgg acccgcccgc agcgggaaga gctcggtgct
     1921 ggtccacctg gcgagcaagg ccgcggaagc cgggctgatg gtcagcgccg cagcgccgcg
     1981 gcgctcgccg ttgcgatcgg cgggtctcgc ccatgtggcc accgaggccg gcgacatcgt
     2041 ctcgttgttc gagtcgtcgt tcgagcaggc cgccggcccc gaccggcgcc ggctgctgct
     2101 ggtcgacgac gccgagctgg tcgacgatcc ttcgggagac atcgaccgaa tcctgtcggc
     2161 acgccggcct gggttgcacg tggtggccgc cggccggagc gacgtgctcc ggaccgcgtt
     2221 cgggcactgg acggcggagg ttcgacggag ccgccagggg ttggtgttac gaccgcagcc
     2281 tgagctggac ggtgagctgt ggcacgtctc gctcccgagg cgatcgccga cccagtggac
     2341 cgccgggcgg ggctacctcg tcatcgacgg cgaggtcgag ctggtccagg cggtggagac
     2401 gcggccagca tgatcaatga acgctacgag ctcgaccggc ccatcggccg cggttcgatg
     2461 ggcgtggtgt accgcgccac gcgcctcgcg gccccgggtg gggtcgtcgc catcaagcgg
     2521 gtcccggtcg cttcggaccc gatgttgcgc gaacgtctgc gggctgaggc cgaggtgctg
     2581 gcgctcctcg accatccgca catcgtccgg gtccacgagg tgatcgacga tggtgacggt
     2641 ctggccgtcg tcatgcccct tgccacgggc gggagcctgg ccgaccggct ggtccgcgag
     2701 ggccgcatgg acgcgaccgt ggtcgcgtcc ttcgccgcgg agatcggtga cgccctgagc
     2761 tgcgcccatc gccagggtgt cctccactgt gacgtgaaac cttccaacat cttgttcacg
     2821 tcggaaggag atgcgctcct gaccgacttc ggcctggccc gctgggtcag ccgtccctcg
     2881 gtatggggcg gcgccgtgat gggcaccgcc gagtacctgg acccggaggt cgccgatggc
     2941 cttcagcccg actcccgcag cgacgtctac tccctggcgg tggtctgtta cgaggcgctc
     3001 accggccggg tcccgttcac ggggcccacc gccctcgccg tgctgaagag ggcggagcgg
     3061 ggtcgagcca cggctgtcgc caacctcgtg cccgatctgc ccgtcgggct ctccgaggcc
     3121 gtggcggagg gcatggcccg gaagaggtcg cgccggcccg agtcggccca ggccttggcc
     3181 gccgcgctgc ggtcgtcggg cacccttgcg cagccgcgag cccggccgtg cgaggcgggc
     3241 ccggggacct cggcaccgcc cgccgcggag atgggctgct cgtcgggccc tcctacggcg
     3301 cgcttcgact tccgccctct cggcccgcac gatcccggcg aggagccccg agccgacgcc
     3361 cgggcgcgca gatctccggc ccggcccgcc ctcgtcgcg
//
LOCUS       DASWGG010000127         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_383767, whole genome shotgun sequence.
ACCESSION   DASWGG010000127 DASWGG010000000
VERSION     DASWGG010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383767"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VF954_02200"
     CDS             complement(<1..754)
                     /locus_tag="VF954_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9529922.1"
                     /translation="MPTVPATSARRPTASRSTEDLVRLYLNEIGRVPVPSPEAQVALA
                     QRIEAGDDAARGQMLAANLRLVVHWASRYQDRGIDLLDLIQEGTFGLAHAIDKFDWRK
                     GFRFSTYASWWIRKSLQQAVAQSATIRVPAEVGEHRRRLARLHDELHEALGRPPTEDE
                     LETESGLSAGEFVRARQTARVAASLDQAVGEEEDSTIGAFVPSKVDVEGAVFDSQAAR
                     ALAQALETLPPLLRRCPAPGPWSAPCGPGAGHR"
     gene            899..>2266
                     /locus_tag="VF954_02205"
     CDS             899..>2266
                     /locus_tag="VF954_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529923.1"
                     /translation="MSAVRSEPYGSWPSPITPALASTGRPSLAAPQLSDGAVWWTEGR
                     PHEGGRVALVRRAAGGPVTDAVSQPWSVRTRAHEYGGGAFRADGAVAYAVNDADQRIH
                     RVAGPDDTTPLTPEPPAPRSLRYADLRVVPGQPWLVAVREAHGVGGVDNCLVAVPTDG
                     SCQLTVLAEGHDFYSFPRPSPDGRQLAWTSWDHPRMPWDGTELWVAALDVGEHGPALA
                     EPARIAGGADESIFQPEWNPAGELHFVSDRSGWWNLYAASPSAAEPLAPLDAEFGLPQ
                     WVFDQATYAFLADGTIACLWSSQGTVHLGLIDRPGVRRELEVPYTSMNGLRAEGSEVV
                     LVGAGPTRAPAIVRLDLGAGQRERPEPTVVSSVAAPALGPGWTSKPEPIRFPTSGGRT
                     AFALHYPPTHPDRTGTGGERPPLIVVSHGGPTAGVDGAYDLRVQFWTTRGFAVVDVAY
                     GGSTGY"
BASE COUNT          286 a          840 c          827 g          313 t
ORIGIN      
        1 gccgatgccc agcgcccggc ccgcacggtg cgctccacgg cccgggcgct gggcatcggc
       61 gaagcaacgg cggcagcgtc tccagcgcct gagccagggc ccgtgcggcc tgggagtcga
      121 agacggcacc ctcgacgtcg accttcgacg gcacgaaggc gccgatggtc gagtcctcct
      181 cctcgcccac ggcctggtcg aggctggccg cgacccgggc cgtctgccgg gcccgtacga
      241 actcgcccgc gctcaggccg ctctctgtct cgagctcgtc ctcggtggga gggcggccga
      301 gcgcctcgtg aagctcgtcg tggagccggg ccaggcggcg cctgtgctcg cccacctcgg
      361 ccggcacgcg gatggtcgcg gactgggcca ccgcctgctg gagcgatttg cggatccacc
      421 agctggcata ggtcgagaac cggaagccct tcctccagtc gaacttgtcg atggcgtggg
      481 ccaggccgaa cgtgccctcc tggatgaggt ccagcaggtc gatgccgcgg tcctggtacc
      541 ggctggccca gtgcaccacg agccgcaggt tggcggccag catctggcca cgagctgcgt
      601 cgtcgccggc ctcgatgcgc tgggcgagcg ccacctgggc ctcggggctg ggcaccggta
      661 cccgaccgat ctcgttgagg tacaggcgca ccaggtcctc ggtcgaccgg ctggcggtgg
      721 gtcgtctggc ggaggtggcg ggaacagtgg gcatggcggc tcgcctggcg gtcgggattg
      781 ctgtcggcat ggctctcctc atcgaaggaa acccggcccc gcaggtgggc attccggccc
      841 cggacgtgac ctacgtcacg ccgaccggca gctcatcggc ctcactacgc tcatgagggt
      901 gagcgccgtt cggtccgagc cctacggcag ctggccctcg ccgatcaccc cggcgctcgc
      961 gtcgaccggg cgcccgagcc tggccgcccc gcaactgtcc gacggagcgg tgtggtggac
     1021 cgagggccgt ccccacgagg gcggccgggt ggcgctcgta cgccgggccg ccggaggccc
     1081 cgtaaccgat gccgtgagcc agccgtggtc ggtgcgcacg cgcgcccacg agtacggggg
     1141 cggcgccttt cgggccgacg gagccgtggc ctacgccgtc aacgacgccg accaacggat
     1201 ccaccgggtc gcgggcccgg acgacaccac ccccctcacc ccggagccgc cggcgccgcg
     1261 ctcccttcgc tacgccgacc tgcgggtggt gccgggtcaa ccgtggctcg tcgcggttcg
     1321 cgaggcccac ggtgtcggcg gggtcgacaa ctgcctcgtg gcggtgccga cggacggatc
     1381 gtgccagctc accgtgctgg ctgagggtca cgatttctac tcgttccccc gccccagccc
     1441 ggacggccgg cagctggcct ggacgagctg ggaccacccc cgtatgccct gggacgggac
     1501 cgagctgtgg gtcgcggcgc ttgacgtcgg ggagcacggg ccggcgctgg ccgagcccgc
     1561 gcgcatcgcc ggcggggccg acgagtcgat cttccagccg gagtggaacc cggccgggga
     1621 gctgcatttc gtgtccgacc ggtccgggtg gtggaacctg tacgcggcgt cgccatcggc
     1681 cgccgagccg ctcgctcccc tggacgccga gttcggcctc ccccagtggg tcttcgacca
     1741 ggcgacctac gccttcctgg ccgacgggac catcgcgtgc ctgtggagca gccaggggac
     1801 ggtgcacctc gggctgatcg accggcccgg ggtgcggcgg gagctcgagg tcccctacac
     1861 atcgatgaac ggcctccgcg ccgaaggctc cgaggtggtc ctggtcgggg ccggaccgac
     1921 ccgggcgccg gcgatcgtcc gcctcgacct cggtgccggg cagagggagc ggcccgagcc
     1981 aacggtcgtc tcgtcggtcg cggccccggc gttgggaccc ggatggacct cgaagcccga
     2041 acccatccgg ttcccgacct ccggaggccg caccgcattc gccctgcact acccgcccac
     2101 ccaccccgac cgcaccggga ccggtggcga acgcccgccg ttgatcgtgg tcagccacgg
     2161 cggcccgacc gccggcgtgg acggggccta cgacctgcgc gtgcagttct ggaccacccg
     2221 cggcttcgcg gtagtggacg tcgcttacgg gggcagcacg gggtac
//
LOCUS       DASWGG010000128         8561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1099068, whole genome shotgun sequence.
ACCESSION   DASWGG010000128 DASWGG010000000
VERSION     DASWGG010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099068"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VF954_02210"
     rRNA            complement(<1..410)
                     /locus_tag="VF954_02210"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1097..3412
                     /locus_tag="VF954_02215"
     CDS             1097..3412
                     /locus_tag="VF954_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX9529924.1"
                     /translation="MCPVRRWARLPLLAVICAGCAWRSQALAPASQVQPESSRILASD
                     GSLLTTLHGPQNRQTVPLAQIPRTLQDAVVATEDSRFWSNDGVDVRALVRAAYANATK
                     GRVVEGGSTITEQYVKNVLLGNDRTVSRKVKEASLAFQLERRYSKPHILELYLNTIYF
                     GNGSYGVQAAADEYFGLPVSQLSLGQSALLAGVVHAPNDADPFLHPDMAAARRRQVLD
                     RMVALGKATPAAAGAAAAEPLIGHPKPPDQHYPAAYFVEQVKRFILDDPRFGPTPEAR
                     RDLLFEGGITVTTTLDRARQALAEDSVSKVLSEPARDPAAALVSLDPHTGAVRALVGG
                     QDFFASSPQAKFDLATQGQRPAGSSFKPFVLAAAIHKGISLATAYDAPPRIDIPLTNG
                     VWHVDNYEGEPSGPMNLVDATVHSINTVYARLILEVGPETAVDLAARMGITTPLHAYP
                     SAVLGTNDVTPLDMAAAYATLANGGVAVTPSFVDQVLDRNGAVLYHRQPDPRRVLDAA
                     TAATVTAVLRQVVDRGTGINARIGRPVAGKTGTGEQWRDAWFVGYTPETVTSVWVGFA
                     DAQQPMQPPATRILVTGGSWPAQIWQLYMSRALATVPVSQFPPPPAGPNGGQSPTTDA
                     GQSGQPDQAGIVVDQVVGMPAQAAEDQLSRAGFRAVTVAVTSDQYPPGYVTSQSPPAG
                     THAAGGSTVTLRVATGPPARAEVPDVVGLTESAARQAIGATGLKVRVVHAAEPPSAGA
                     AGRKGLVWKQDPPGDTATLATTTVTIDVNPA"
     gene            complement(3465..4061)
                     /locus_tag="VF954_02220"
     CDS             complement(3465..4061)
                     /locus_tag="VF954_02220"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX9529925.1"
                     /translation="MVEPGCPATAALPRSFYRRDPREVAPALLGQTLVRGGRAGRVVE
                     VEAYCGSEDPGSHAYRGRTARNATMFGPPGHLYVYFTYGMHWCANAVCGDEGTGWAVL
                     LRALAPVGGMDEMRAARPAARRERDLCSGPAKLCQAFGIDRSMDGADLVSGSLGIRIC
                     RGARVPAGAVVQTTRVGLSAGAEHPWRWYLAGEHVSRR"
     gene            complement(4054..5436)
                     /gene="argH"
                     /locus_tag="VF954_02225"
     CDS             complement(4054..5436)
                     /gene="argH"
                     /locus_tag="VF954_02225"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX9529926.1"
                     /translation="MTLWHGRFVSGPAEELMAFNRSLAFDQRLAEDDLVGSRAHVRGL
                     GRAGILTKEEVDVLMTALDHVAEELAEGRLAFLDSDEDIHTVIERRVTEIAGETGAKL
                     HTGRSRNDQVATDLRLYARRALRQVARRVLGLQEVLLERSARAGDAYLPGYTHLQRAQ
                     PVLLAHHLLAHGWALSRDIDRLLSTVQRLDVSPLGAGALAGSSLPLDPDAVAADLGFG
                     RRFENSLDAVSDRDFVAEALFDLALLGVHLSRLGEEIVLWSTEEFGFMHLADAYSTGS
                     SMLPQKKNPDIAELVRGKSGRLIGHLTGLLATLKGLPLAYNKDLQEDKEPLFDAIDQL
                     MVALSALRGLLETATLDLDRMRQAADTSTSAAVDLAEWLVERGMPFRQAHVVVGGLVR
                     DSIERHVPLAELVESHPALGTDALPLLEPGVAVSRRVTPGGAGPGPVAEQLQRFRGRL
                     RLDAERIGDG"
     gene            complement(5453..6700)
                     /locus_tag="VF954_02230"
     CDS             complement(5453..6700)
                     /locus_tag="VF954_02230"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX9529927.1"
                     /translation="MAKRVVLAYSGGLDTSVAVRWMGEELGVEVIAVAVDVGQGGDFE
                     AIRQRALAAGAADAVVVDCREEFAADFVAPALRANAKYEGKYPLVSALSRPVIVKHLV
                     EQAREHGADAVAHGCTGKGNDQVRFEVSTAALAPDLETIAPVRGWGLTREQSIEYAER
                     FGIPITVTRSSPYSIDQNLWGRTIECGVLEDPWQQPPEEVFELTTPTASSPTEMVVRF
                     EAGLPVALDGVAMALPSLIGEVTRVVGSYGWGRIDMVENRRVGIKSREVYECPGALAL
                     LMAHADLEDLTLERDVAHEKARIEPRYADLVYDGLWFSPLKAALDAFILESQRYVTGE
                     VRLRCELPGRCFVAGRRSEVGLYDYGLATYEAADAFRHQDAEGFVRLWGLGVQTWAAR
                     QARPGGSGAAVGQPGAAGPAGPA"
     gene            complement(6734..7234)
                     /gene="argR"
                     /locus_tag="VF954_02235"
     CDS             complement(6734..7234)
                     /gene="argR"
                     /locus_tag="VF954_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX9529928.1"
                     /translation="MARQPAGALMAKNQRQHRIARILETSAVASQAQLVELLGGEGVV
                     ATQATVSRDLEDLGAVKVRVPGGETVYAIPEVAKDRIAPSDHLRRVLGDWVVDVGASG
                     NLVVLRTPPGSAHVVASALDRSGLEDVLGTVAGDDTILVVAAPGVPGTAVAGYLAGLA
                     GVEPRT"
     gene            complement(7207..8124)
                     /gene="argF"
                     /locus_tag="VF954_02240"
     CDS             complement(7207..8124)
                     /gene="argF"
                     /locus_tag="VF954_02240"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX9529929.1"
                     /translation="MARHLLEIDDLAPAELDSVLDLAERAGPPAVLAGRGVALVFEKP
                     SARTRSSMEMAVVQLGGHPVTIRGDEVGLDTRETTEDVARTLACFHAVIAARVLAHTT
                     LERMVSVLEGEGHSPVPVVNLLSDQGHPVQALADLLTLRQHFGRLEGLTIAFVGDGNN
                     VARSLALGCALSGATFRIASPDGFALPLAEVARVAAIGGAIDTFSSPPEAVAGADAVY
                     TDTWTSMGQEGEAEDRRAAFAGYRVDRALMDRAAKGAVFLHCMPAHRGEEVASEVMDG
                     PRSLIWQQAENRMHAARGLLWWLANRRGR"
     gene            complement(8130..>8561)
                     /locus_tag="VF954_02245"
     CDS             complement(8130..>8561)
                     /locus_tag="VF954_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9529930.1"
                     /translation="MPIGACWARAEVAAAFVPGDHGSTFGGQPLAAAAARAVLAVMER
                     DDVPARAARAGRRLQAALAAVPGVRAVRGLGLLVAVELDGADAKAVAAACLDAGLVVN
                     AVTGTALRLAPPLLVSDEEIDEAVAVLTGVLATTAAAGAGG"
BASE COUNT         1232 a         3264 c         2941 g         1124 t
ORIGIN      
        1 cacgcggcgt tgctgcgtca ggctttcgcc cattgcgcaa gattccccac tgctgcctcc
       61 cgtaggagtc tgggccgtgt ctcagtccca gtgtggctga tcgtcctctc agaccagcta
      121 cccgtcgaag ccttggtgag ccgttacccc accaacaagc tgataggccg cgagcccctc
      181 ccaaagcaag attcatttcc tcatcaggcc atgcgacccg atgggggcat ccggtattag
      241 ctccggtttc ccggggttat cccagtcttt ggggcaggtt gctcacgtgt tacgcacccg
      301 ttcgccactc ggtcttcacc agggttgccc ctggttggac ctcgttcgac ttgcatgtgt
      361 taagcacgcc gccagcgttc gtcctgagcc aggatcaaac tctccatcga gatctgcgac
      421 ccgcccgaag gcgaggccag agatcagaag agccggccgt cccgggcacc aacccggtcg
      481 ggccaactgg cacagaaaca taaacaatgg tttgtgattg ttgtccgttt cagtgcattg
      541 actatttgca accgcggcaa ctcgcaagcg agccacccgg ttgcccgcac tggcttttgg
      601 ctatctctgt tccgttttca aggagcaccg tggtggaggc acgcgccaac ccagatgagg
      661 attggtgtcg gtgcgaaccc accctgtggc gagggttgcg ggcggtgccc gtacttccgt
      721 cgcgtgggcc tcggacgcag atgctgctcg ttcgaggcgg ctagccagac tacgcccaac
      781 cccgattggc tgtcaactga gcgccccccg ctccaactgg gcgcgccccg ctacccggtg
      841 tgtcgcccag cgcgcccaga acgctaccgc cccgcgttcc ccccggtccg gagaaagcgc
      901 cccgcgtgcc acacagctcc gtggggcggc gcggatcggg ccagacggcc ccggcgaggc
      961 ggcccggcca ccgccctcgc ccaactgggc gcgcccaggt acacagcgcg tcaccgggcg
     1021 cgcccagaag cggcggggcg ctgaccaggg accatgcccc agtcgcaccc ctcccgcccg
     1081 cgacaatgaa cacccgatgt gtcccgtgcg ccgctgggcc cgcctgcccc ttctggccgt
     1141 gatctgcgcc ggctgcgcct ggcgcagcca ggcgctcgcc cccgcgtccc aggtgcagcc
     1201 cgagtcatcc cgcatcctcg catccgacgg ctcgctgctc accaccctcc acgggcccca
     1261 gaaccgccag accgtccccc tcgcccagat cccccggacg ctccaggacg cagtggtggc
     1321 gaccgaggac agccggttct ggagcaacga cggggtggac gtccgcgccc tggtgcgggc
     1381 ggcctacgcc aacgccacca agggcagggt cgtcgagggg ggctccacca tcaccgagca
     1441 gtacgtcaag aacgtgctgc tcggcaacga ccgcacggtc agccgcaagg tcaaggaggc
     1501 gtcgctcgcc ttccagctgg agcggcggta ctccaagccc cacatcctgg agctctacct
     1561 gaacaccatc tacttcggca acgggtccta cggggtccag gccgccgcgg acgagtactt
     1621 cgggctcccc gtaagccagc tcagcctggg ccagtccgcc ctgctggccg gggtcgtcca
     1681 cgcgcccaac gacgccgacc ccttcctgca cccggacatg gccgccgccc ggcgccgcca
     1741 ggtcctcgac cggatggtgg ccctcggcaa ggccactcct gcggcggccg gtgccgcggc
     1801 cgccgagccc ctcatcggcc accccaagcc accggaccag cactaccccg ccgcctactt
     1861 cgtcgagcag gtcaagcggt tcatcctgga cgatccccgg ttcgggccca cgcccgaggc
     1921 gcgccgggac ctattgttcg agggcggcat caccgtgacc accaccctcg accgcgcccg
     1981 ccaggccctg gccgaggact ccgtgagcaa ggtcctctcc gagccggcgc gcgatccggc
     2041 cgcggcgctg gtgtccctcg acccacacac cggggcggtg cgggccctgg tcggcggcca
     2101 ggacttcttc gccagctcgc cccaggccaa gttcgacctg gccacccagg gacagcgccc
     2161 cgcgggctcc agcttcaagc ccttcgtgct ggccgcggcc atccacaagg ggatctcgct
     2221 cgccaccgcc tatgacgccc cgccccggat cgacatcccg ctgacgaacg gcgtgtggca
     2281 cgtcgacaac tacgagggcg agcccagcgg cccgatgaac ctggtcgacg ccacggtcca
     2341 ctccatcaac accgtgtacg cccgtctcat cctcgaggta gggccggaga ccgcggtcga
     2401 cctggcggca cggatgggca tcaccacccc gctgcacgcc tacccgtccg ccgtgctcgg
     2461 caccaacgac gtgaccccgc tcgacatggc cgcggcgtac gccaccctgg ccaacggcgg
     2521 ggtcgcggtg acgcccagct tcgtggacca ggtgctcgat cggaacggcg cggtgctcta
     2581 ccaccgccag cccgacccgc gccgggtcct cgacgccgcc acggccgcga cggtcaccgc
     2641 ggtcctccgc caggtcgtgg accggggcac gggcatcaac gcacggatcg gccggccggt
     2701 ggccggcaag accggtaccg gcgagcagtg gagggacgcg tggttcgtgg gctacacgcc
     2761 cgaaacggtc acctcggtgt gggtcgggtt tgccgacgcc cagcagccga tgcagccacc
     2821 ggccacccga atcctcgtca ccggcgggtc gtggccggcg cagatctggc agctctacat
     2881 gtccagggcg ctcgcgaccg tgccggtgag ccagttcccg cccccgcccg cgggcccgaa
     2941 tggggggcag agtcccacga ccgacgcggg gcagagcggc caacccgacc aggcgggcat
     3001 cgtcgtggat caggtcgtgg gcatgccggc ccaggcggcc gaggaccagc tgtcccgagc
     3061 gggcttcaga gccgtgacgg tggcggtcac cagcgatcag tacccgccgg gctacgtgac
     3121 cagccagagc ccgccggcgg gcacccacgc agccggcggc tccaccgtca ccctgcgcgt
     3181 ggcgaccggc cctcccgccc gggccgaggt ccccgacgtg gtcggcctga cggagtccgc
     3241 cgcgaggcag gccatcgggg ccacggggct caaggtccgc gtggtccacg cggccgagcc
     3301 ccccagcgcg ggggcagccg ggcgcaaggg cctggtctgg aagcaggacc cgccggggga
     3361 tacggccacg ctcgccacca ccacggtgac catcgacgtc aatccggcgt agcggggtgg
     3421 cgccgccgtt ccgtcgggat gtgagccgcc cgagccgacg ggggtcagcg ccgggacacg
     3481 tgctcccccg ccaggtacca ccgccaggga tgctcggctc ccgccgacag cccgacccgg
     3541 gtcgtctgca ccaccgcgcc ggccgggacc cgtgcgcccc ggcagatccg gataccgagg
     3601 gagccgctca ccaggtcggc cccgtccatc gagcggtcga tgccgaacgc ctggcacagc
     3661 ttggccgggc cgctgcagag gtcgcgctct cgccgcgccg cgggccgggc ggcgcgcatc
     3721 tcgtccatgc cgccgaccgg agccaaagcg cgcaggagca cggcccagcc cgtgccctcg
     3781 tcgccgcaca cggcgttggc gcaccagtgc atgccgtagg tgaagtacac gtacaggtgc
     3841 cccggcggcc cgaacatggt cgcgttgcgc gccgttcggc cccggtaggc gtggctgccg
     3901 gggtcctccg acccgcagta ggcctcgacc tccacgacac gccccgcccg gccgccccgc
     3961 accagcgtct ggcccagcag cgccggcgcc acctcacggg ggtcgcgccg gtagaaggac
     4021 cgtggcaggg ccgcggttgc cgggcagcca ggctcaacca tcgccgatgc gctcggcgtc
     4081 gaggcgcagc cggccgcgga agcgctggag ctgctcggcc acggggccgg ggccggcacc
     4141 gcccggcgtg acccgccggg acaccgccac cccgggctcg agcaggggca gggcgtcggt
     4201 gccgagcgcc gggtgggact cgaccagctc cgccagcggc acgtggcgct cgatggagtc
     4261 acggaccagg ccaccgacca cgacgtgggc ctggcggaag ggcatgcccc gctcgaccag
     4321 ccattcggcg aggtcgacgg ccgcggacgt ggaggtgtcg gccgcctggc gcatgcgatc
     4381 gaggtccagg gtggcggtct ccagcaggcc ccgcagcgcg ctcagcgcga ccatcagctg
     4441 gtcgatcgcg tcgaaaagcg gttccttgtc ctcctggagg tccttgttgt aggcgagcgg
     4501 caggcccttg agagtggcca gcagcccggt gaggtggccg atgaggcggc cgctcttgcc
     4561 ccgtaccaac tcggcgatgt ccgggttctt cttctgcggg agcatcgagc tgccggtcga
     4621 gtaggcgtcg gcgaggtgca tgaagccgaa ctcctccgtc gaccacagga cgatctcctc
     4681 gcccaggcgc gacaggtgca caccgagcag ggccaggtcg aagagggctt cggccacgaa
     4741 gtctcggtcg ctgacggcgt cgaggctgtt ctcgaagcgc ctgccgaagc cgaggtcggc
     4801 ggccaccgcg tcggggtcga gcggaagcga cgagccggcc agggcgccgg cgccgagcgg
     4861 cgacacgtcg aggcgctgca cggtggacag cagccggtcg atgtcgcgcg acagcgccca
     4921 cccgtgggcc aggagatggt gggcgaggag cacgggctgg gcccgctgga ggtgggtgta
     4981 gcccgggagg taggcgtcgc cggctcgcgc ggaccgttcc agcagcacct cctggagccc
     5041 cagcacccgg cgggccacct ggcgcaaggc ccgacgtgcg tagaggcgaa ggtccgtggc
     5101 cacctggtcg ttgcggctgc ggccggtgtg cagcttggcg ccggtttctc cggcgatctc
     5161 ggtcacccgc cgctcgatga cggtgtggat gtcctcgtcg ctgtcgagga aggcgaggcg
     5221 gccctcggcc agctcctcgg ccacgtggtc gagggcggtc atcaggacgt cgacctcctc
     5281 cttggtcagg atgccggccc gcccgagccc gcggacatgg gcccgggagc cgaccaggtc
     5341 gtcctcggcc aggcgctggt cgaaggccag gctccggttg aaggccatca gctcctccgc
     5401 cggaccgctc acgaagcggc cgtgccacaa cgtcatcgcc gcagtccggt cgtcacgccg
     5461 gcccagccgg ccccgccgcg cctggctgac cgaccgcggc tcccgagccc cccgggcgag
     5521 cctgacgagc tgcccaggtc tggaccccga gcccccacag ccgcacgaac ccctccgcgt
     5581 cctggtgacg gaaggcgtcg gccgcctcgt aggtggccag gccgtagtcg tacaggccga
     5641 cctcgctgcg ccggccggcc acgaagcagc ggcccggcag ctcgcagcgc agccgcacct
     5701 cgcccgtcac gtaccgctgg ctctccagga tgaaggcgtc gagcgccgcc ttcaggggcg
     5761 agaaccacag cccgtcgtac accaggtccg cgtaccgggg ctcgatcctg gccttctcgt
     5821 gggccacgtc gcgctcgagc gtgaggtcct cgagatcggc gtgggccatc agcagggcga
     5881 gcgccccggg gcactcgtag acctcccggc tcttgatccc cacccgccgg ttctccacca
     5941 tgtcgatccg gccccagccg taggagccca cgacccgggt cacctcgccg atcaaggacg
     6001 gcaacgccat ggcgaccccg tccagcgcca cgggcaggcc cgcttcgaac cgcaccacca
     6061 tctccgtggg actcgacgcg gtgggagtgg tgagctcgaa gacctcctcc ggcggctgct
     6121 gccacgggtc ctccaggacg ccgcactcga tggtccggcc ccacaggttc tggtcgatcg
     6181 agtaggggct cgacctggtc acggtgatgg ggatcccgaa gcgctcggcg tactcgatcg
     6241 actgctcccg ggtgaggccc cagccccgca ctggcgcgat ggtctcgagg tcgggggcca
     6301 gggcggcggt cgacacctcg aagcgcacct ggtcgttgcc cttcccggtg cagccgtggg
     6361 cgaccgcgtc ggcaccgtgc tcgcgggcct gctcgaccag gtgcttgacg atgacgggcc
     6421 gggacagcgc cgacaccaac gggtacttgc cctcgtactt ggcgttggcc cgcagggccg
     6481 gggccacgaa gtcggcggcg aactcctccc ggcagtcgac cacgacggcg tcggcggccc
     6541 cggcggcgag ggcgcgctgg cggatggcct cgaaatcgcc accctggccg acgtcgacgg
     6601 cgacggcgat gacctcgacc ccgagctcct cacccatcca tcgcaccgcg accgaggtgt
     6661 ccaacccccc gctgtaggcc agcactaccc gcttcgccac tggtctgccc tttctcccaa
     6721 atcgatcagg tcctcacgtt cggggctcga cgccggccag cccggccagg tacccggcca
     6781 ccgcagtgcc cggcacgccc ggcgcggcca ccacgaggat ggtgtcgtca ccggcgaccg
     6841 tgcccagcac gtcctccagc cccgaacggt ccagggcgga cgcgacgacg tgcgccgagc
     6901 cgggcggggt ccgcagcacc accaggttcc cggacgcgcc gacgtcgacc acccagtcgc
     6961 ccaggacccg acggaggtgg tcggagggcg cgatgcggtc cttggccacc tcggggatgg
     7021 cgtacacggt ctcgccgccc ggcacccgca ccttgacggc gccgagatcc tctaggtccc
     7081 gcgacaccgt cgcctgtgtg gccacgaccc cttcgcctcc gagcagctcg acgagctgcg
     7141 cctggctggc gacggcgctg gtctccagga ttcgggcgat gcggtgctgg cgctggttct
     7201 tggccatcaa cgcccccgcc ggttggcgag ccaccacagc aggccgcgcg ccgcgtgcat
     7261 gcggttctcg gcctgctgcc agatcaggct ccggggcccg tccatcacct cggaggccac
     7321 ctcttcaccc cggtgagccg gcatgcagtg caggaagacc gctcccttgg cggcccggtc
     7381 catcaacgcc cggtcgaccc ggtagccggc gaaggccgcc cgccggtcct cggcctcacc
     7441 ctcctggccc atcgaggtcc acgtgtcggt gtacaccgcg tcagccccgg cgacggcctc
     7501 gggcggcgag ctgaacgtgt cgatggcccc gccgatggcc gccacccggg ccacctcggc
     7561 aagaggaagg gcgaagccgt ccggggaggc gatccggaag gtggcgcccg acagcgcgca
     7621 gcccagggcg agggagcggg ccacgttgtt gccgtccccc acgaaggcga tggtgaggcc
     7681 ctccagccgg ccgaagtgct ggcgcagcgt aagcaggtcg gcgagcgcct ggacggggtg
     7741 gccctggtcg gagagcaggt tcacgaccgg gaccggcgag tggccctccc cctcgaggac
     7801 gctcaccatt cgctccagcg tggtgtgcgc gagcacccgc gccgcgatga cggcatggaa
     7861 gcaggccagg gtccgcgcca cgtcctcggt cgtctcccgg gtgtcgagac cgacctcgtc
     7921 gccgcggatg gtcaccgggt ggccgccgag ctgcacgacg gccatctcca tggagctgcg
     7981 ggtccgggcc gacggcttct cgaagaccag cgccacccct cgcccggcca gcacggcggg
     8041 agggccggcg cgctcggcca ggtccagcac gctgtcgagc tcggcaggag ccaggtcgtc
     8101 gatctccagc aggtggcggg ccacgggcgt cagccccccg ccccggcggc cgcggtggtg
     8161 gccagcacgc ccgtgagcac ggcaaccgcc tcgtcgatct cctcgtcgct cacgagcagc
     8221 ggcggggcca ggcgcagggc ggtcccggtc acggcgttga cgaccaggcc ggcgtcgagg
     8281 caggcggcgg ccacggcctt ggcgtcggcg ccgtccagct ccaccgccac cagcagcccg
     8341 aggccccgca ccgctctcac cccgggcacg gcggccagag cggcctgcag gcgtcgaccg
     8401 gcccgggcgg cccgggcagg cacgtcgtcg cgctccatca cggccagcac ggcccgggcc
     8461 gcggccgcgg ccagcggctg cccgccgaag gtgctgccgt ggtcgccggg cacgaaggcg
     8521 gccgccacct cggcccgggc ccagcacgcg ccgatcggca t
//
LOCUS       DASWGG010000129         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1414297, whole genome shotgun sequence.
ACCESSION   DASWGG010000129 DASWGG010000000
VERSION     DASWGG010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414297"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VF954_02250"
     CDS             complement(<1..1036)
                     /locus_tag="VF954_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529931.1"
                     /translation="MDRATTPRTAAERDVPDVAGLVRAACGVDPGQAEASDVIIAAVE
                     NGLPLGVLASQAATVDDASWAGPLEAEAARAAVRAATMEVLAQCLNPTDRVAPCTLPW
                     RPSSGRDIDLAVAASDFTRVVEVLDATALVPVAGWRTPTEAGWLRLDEGRVVDLIEAQ
                     AVADRDLVVTEQGWRLNLDAQLMHTTGGVARRGAVRLVDLADLAWLRRQPGFALEGVS
                     TSRGADMVEGAERALELGSASFPWRPPSSVTSRGRRLVRQLARRRPPRLAFSGIDGSG
                     KTSHAWALAGSLERAGVPVRTLWNRPGFELRALERVARTAKAVLGDRSTTPALARQLA
                     DDPQAIDARSR"
     gene            complement(1075..>2052)
                     /locus_tag="VF954_02255"
     CDS             complement(1075..>2052)
                     /locus_tag="VF954_02255"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX9529932.1"
                     /translation="YDLVFGGDDVEVLALSAVRDEVPALFPYGPDATVRRSFDKCELT
                     RAAARAGLAAPPTELHRPDPGWAFPVVVKARLHWTPGAASAEPRMPARVCQSEHDLGE
                     RVAEIEHQGGEAVVQPVVAGQLMAFTALCDRSATIVARSQQRAEALSPHWGTSTLAVT
                     ELVDPALAEGVQRLLTDLGWFGLANLQFLRQPAGEALLIDFNGRFYGSLALSVAAGVS
                     FPRLWADLATGRWTPAGGVVTARPGVRYQALENDLRRAFAERRRGLAVDLLGCALRGL
                     TAAHSTWSWRDPVPGLGRAGAVLSGQLRARVGAAAKGRTRAGRRPHQSG"
BASE COUNT          291 a          781 c          726 g          254 t
ORIGIN      
        1 tgcgtgatcg tgcgtcgatt gcctgggggt cgtcggccag ctggcgagcc agcgcgggcg
       61 tggtcgagcg gtcgccgagc acggccttgg ccgtccgggc gacgcgctcg agggcgcgga
      121 gctcgaagcc gggtcggttc cagagcgtgc ggaccgggac gccagctcgt tcgaggctgc
      181 ccgcgagcgc ccacgcatgg ctggtcttgc cggacccgtc gatgccgctg aaggccagtc
      241 ggggtggtcg ccgacgcgcc agttgacgga cgagccgtcg cccgcgggag gtcacgctgc
      301 tcggcgggcg ccagggaaag ctggccgagc ccagctcgag ggcgcgctcg gcgccctcga
      361 ccatgtcggc gccacggctg gtgctcacac cctccagagc gaacccgggc tgtcgccgca
      421 gccaggccag atcggcgagg tcgaccagcc gcacggctcc tcgccgggcc acacccccgg
      481 tcgtgtgcat cagctgcgca tccaggttca gccgccaacc ctgctcggtg accacgaggt
      541 cccgatcggc gacggcctga gcctcgatca agtcgacgac cctcccctcg tccagccgga
      601 gccagccggc ctcggtcggg gtgcgccagc cggccaccgg taccagggcg gttgcatcga
      661 gcacctcgac caccctcgtg aagtcgctgg cggccacggc caggtcgatg tctcggccgc
      721 tcgaaggtcg ccacggcaac gtgcacggcg cgacccggtc ggtggggttc aggcactgtg
      781 ccagcacttc catcgtcgcg gcccggaccg cggcccgggc cgcctcggcc tcgaggggcc
      841 ccgcccagga ggcatcgtcg acggtcgccg cctgcgaggc gagcacaccg agcgggagcc
      901 cgttctcaac ggccgcgatg atcacgtccg aggcctccgc ctggcccggg tcgacgccac
      961 aggccgcccg gacgagcccg gcgacgtcgg ggacgtcgcg ctcggcggcc gtccgcggcg
     1021 tcgtcgcacg atccattcgg caattatcac cgcccgctcg cccggctacc ggattcaacc
     1081 ggactgatgg ggcctgcggc ccgctcgcgt ccgtcccttc gcggccgctc ccacccgggc
     1141 gcggagctgg cccgaaagga ccgcgccggc ccggcccaac ccgggcacgg ggtcccgcca
     1201 cgaccaggtg gagtgagcgg cggtgagccc gcgaagggcg caacccagca ggtcgacggc
     1261 gagcccccgt cggcgctcgg cgaaggcacg gcggagatcg ttctccaacg cctgataacg
     1321 caccccgggc ctggctgtca cgaccccgcc tgccggtgtc cagcgccccg tagccagatc
     1381 ggcccacagc cggggaaagc tcacgccggc tgcgaccgac agggccagcg agccgtagaa
     1441 ccgtccgttg aaatcgatca acagtgcctc acccgccggt tgcctgagga actggaggtt
     1501 ggccagcccg aaccacccaa ggtccgtgag cagccgttgc acgccctcgg cgagggccgg
     1561 atcgaccagc tcggtcacgg ccaaggtgct ggtcccccag tggggcgaca gcgcctcggc
     1621 gcgctgctgg cttcgggcga cgatcgtggc cgaccggtcg cacaacgcgg tgaacgccat
     1681 cagctggccg gcgacgacgg gctgcaccac cgcctccccg ccctggtgct cgatctcagc
     1741 cacccgttcg cccagatcgt gctcggattg gcacacccgc gccggcatcc ggggctcggc
     1801 actggcggcg cccggcgtcc agtgcagccg ggccttgacc acgaccggga aagcccatcc
     1861 cggatccggc cggtgcagct cggtcggtgg agccgccagc ccggctcgcg ccgcggcgcg
     1921 cgtgagctcg catttgtcga aggagcgccg cacggtggcg tcgggcccgt acgggaacaa
     1981 cgccggcacc tcgtcgcgga ccgcggacag ggccagcacc tcgacatcgt ccccgccgaa
     2041 gaccaggtcg ta
//
LOCUS       DASWGG010000130         5851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1103449, whole genome shotgun sequence.
ACCESSION   DASWGG010000130 DASWGG010000000
VERSION     DASWGG010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103449"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="VF954_02260"
     CDS             complement(<1..1165)
                     /locus_tag="VF954_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9529933.1"
                     /translation="MFDDVTAAELRRRVGTKWRRHGPDVIEAWVAEMDFPPAPAIADA
                     VADAVRRGDAGYPPLEAESDLPAALCDWLRHVYGWSVDPATVFVTSDLLRALETCLDL
                     ITPPGTPVVLPTPSYPPFFEVLALASRPVVDVPMAGCDGRKSDGGAPTLDLAGIEDAL
                     SGGADAIILCNPHNPLGRAFDRDELLALSDVASAYGARVIADEVHAPLVYPGRVHVPF
                     ASLSARAAEQSVTLTSASKGWNIPGLRCAQVVLTNPTDAAAWRKLSWLRTQGAATPGI
                     VANVAAYRSGGPWLGDAVAYLDANRHLLAQLLREFLPELRHRTPEAGYLAWIDCRALP
                     VADPAGFFLRQAKVALHDGALFGPAGKGFVRLNFATSAAILEQMVEAMAGAVRR"
     gene            complement(1235..2071)
                     /locus_tag="VF954_02265"
     CDS             complement(1235..2071)
                     /locus_tag="VF954_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX9529934.1"
                     /translation="MGNPRELTLMAVHAHPDDESSSTGGVLARYSAEGIRTVVVTCTN
                     GEYGDGPGHVKPGADGHDPEQVAKTRLAELELACEHLGVWRSELLGYHDSGMPDWAFR
                     EDDHVFWNADPAEPVARLVELFERHRPDVVVTYDDNAGYNHPDHLRANHVTIAAIEAT
                     DIPSKAYLIARRRHDWDGLRERAQAAGVELPTFPSQTPERIQQAAEREAQITTSIDVS
                     AVVGSKRAALASHASQLDQAWWVDFPDELFAELFGRESFIRYRDRTGAPVPEDDLFAG
                     LR"
     gene            complement(2145..3425)
                     /locus_tag="VF954_02270"
     CDS             complement(2145..3425)
                     /locus_tag="VF954_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9529935.1"
                     /translation="MELLRFATAGSVDDGKSTLIGRLLYDAKSIFEDQLEAVERSSRQ
                     RGLEHTNLALLTDGLRAEREQGITIDVAYRYFATPRRKFIIADTPGHVQYTRNMVTGA
                     STADLAVVLVDARNGVLEQSRRHAFLASLLRIPHLVVCVNKMDLVGWDETVFDAIAAD
                     FAAFAGGLDVHEVTYIPISALRGDNVVDPSINMPWYAGPPLLRHLEEVEIASDRNLCD
                     ARFPVQYVIRPFSDEHHDYRGYAGAVAGGVFRLGDPVVVLPSGQRSTVAGIDTLDGPI
                     GEAFPPMSVTIRLSDDIDVSRGDVVVGDGLAPRVERSLRADVCWMTERPLTTRGRYLL
                     KHTTRTTKAVVESIEWRLDVNSLEHHEPAAGGLALNEIGRVELRVATPLVFDPYSRNR
                     TTGSFILVDEATYATVGAGMIVDPGGAGGGPPST"
     gene            complement(3444..4415)
                     /gene="cysD"
                     /locus_tag="VF954_02275"
     CDS             complement(3444..4415)
                     /gene="cysD"
                     /locus_tag="VF954_02275"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX9529936.1"
                     /translation="MTMTLTPTAAPAPRPAPEPPCPSPFELTHLDALEAEAVFIMREV
                     AAELERPVLLFSGGKDSIVLLRLAEKAFRPGRFPFPVMHVDTGHNFPEVLEFRDRRVA
                     ELGERLIVAQVQDSIDQGRVVEELGPRASRNRLQTTTLLDTIEAQGFDAAFGGARRDE
                     EKARAKERVLSFRDDFGQWDPKHQRPELWELYNGRIRRGEHIRAFPISNWTELDVWQY
                     IARERLEVPSIYFAHRRQVFARDGMLLAVSPHVTPLPEEDVLDATVRYRTVGDMSCTG
                     AVLSEASDIDAIIEEIAATRITERGATRADDKFSEAAMEDRKREGYF"
     gene            complement(4432..5100)
                     /locus_tag="VF954_02280"
     CDS             complement(4432..5100)
                     /locus_tag="VF954_02280"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX9529937.1"
                     /translation="MTDVLIAPDDVPALAASFETAPPGRVLRWAGDAFGSSLALASSF
                     EDPVLLHMAAAAVPGIEVLFLDTQFHFAETLWYMEELRAQLGLNITVMRPTVERSNLW
                     QSDLAGCCGIRKVEPLNRGLAGKAAWVTGLRRVDAPTRATTPVVEWDARRAMVKINPL
                     ATWTDEDMAGYISDHQLLTHPLAERGFGSIGCWPCTRPVAPGEDRRAGRWAGSDKTEC
                     GLHV"
     gene            complement(5097..>5851)
                     /locus_tag="VF954_02285"
     CDS             complement(5097..>5851)
                     /locus_tag="VF954_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529938.1"
                     /translation="GTVSAYAWCRLGDVTAEQFRAVATIQRDLGVDVRVTNRQNFVVR
                     GLSELQLPDLFARLDAAGMAAPGAELARDVVSCPGADTCNLAVTQSRGLADDIGRSLE
                     EAGLADIGGVRINISGCTNSCGQHHVSDIGFMGVERRAHGRSAPGYQMLLGGHVGDAR
                     IEFGAKALRLPAKRVGEATVVVVRRYEEERTPGEAFQSWLSRSGGASAVGASLKELDV
                     FPSPEERPDFYIDFDEQGPYVADVGEGECAAS"
BASE COUNT          807 a         2013 c         2219 g          812 t
ORIGIN      
        1 ctcggcgcac ggcaccggcc atggcctcga ccatctgctc gaggatggcg gccgaggtgg
       61 cgaagttgag ccggacgaag cccttcccgg ccgggccgaa gagcgccccg tcgtgcaggg
      121 ccaccttggc ctggcgcagg aagaaccccg ccgggtcggc caccggaagg gcccggcagt
      181 cgatccaggc caggtagccg gcctcggggg tgcggtggcg cagctccggg aggaactcgc
      241 gcagcagctg ggccagcagg tggcggttgg cgtcgaggta ggccaccgcg tcgcccagcc
      301 agggtccccc cgaccggtag gcggccacgt tggccacgat gcccggtgtg gccgccccct
      361 gggtgcgcag ccaggacagc ttgcgccacg ccgccgcgtc ggtggggttg gtgagcacga
      421 cctgggcgca gcgcaggccg gggatgttcc agcccttgga cgccgaggtc agggtgacgg
      481 actgctcggc cgcccgggcc gacaggctgg cgaagggcac gtgcacgcgg ccggggtaga
      541 cgagcggggc gtggacctcg tcggcgatca cccgggcgcc gtaggccgag gccacgtccg
      601 aaagcgcgag cagctcgtcg cggtcgaacg cccggcccag cgggttgtgc gggttgcaca
      661 ggatgatggc gtcggcgcct ccggacaggg cgtcctcgat gccggccagg tcgagggtcg
      721 gcgccccgcc gtcactcttc cggccgtcgc agcccgccat gggcacgtcg acgacgggcc
      781 ggctggcgag ggccagcacc tcgaagaacg gcgggtacga cggggtgggc agcactaccg
      841 gggtgccggg cggcgtgatc aggtccaggc acgtctccag ggcgcgcagc aggtcggacg
      901 tgacgaagac ggtggccgga tcgacgctcc acccgtagac gtggcgtagc cagtcgcaca
      961 gggcggcggg caggtcgctc tctgcctcca ggggcgggta gccggcgtca ccgcggcgca
     1021 cggcgtcggc gacggcgtcg gcgatcgcgg gcgcgggcgg gaagtccatc tcggccaccc
     1081 acgcctcgat gacgtcgggg ccgtggcggc gccacttggt ccccacccgc cggcgcagct
     1141 ctgcggccgt cacgtcgtcg aacatgggcc ccgagcctag gagcgggcac cccccggtgg
     1201 ccctccggcg ccgggggcgg ttactgcaag ccggttaccg caagccggcg aagaggtcgt
     1261 cctcggggac gggcgccccg gtgcggtcgc ggtagcggat gaagctctcg cgcccgaaca
     1321 gctcggcgaa cagctcgtcc gggaagtcca cccaccacgc ctggtcgagc tggctggcgt
     1381 gggacgccag ggcggcgcgc ttggagccga ccaccgccga cacgtcgatg gacgtggtga
     1441 tctgcgcctc tcgctccgcc gcctgctgga tgcgctccgg tgtctgggac gggaaggtgg
     1501 gcagctcgac gccggccgcc tgcgcccgct cgcgcaggcc gtcccagtcg tggcgccggc
     1561 gggcgatgag gtaggccttg gacgggatgt cggtggcctc gatggcggcg atggtgacgt
     1621 ggttggcgcg caggtggtcg ggatggttgt agccggcgtt gtcgtcgtag gtcaccacca
     1681 cgtcgggccg gtgccgctcg aacagctcga cgagccgggc caccggctcg gccggatcgg
     1741 cgttccagaa gacgtggtcg tcctcccgga aggcccagtc gggcatgccc gagtcgtggt
     1801 agcccaacag ctccgagcgc cacacgccga ggtgctcgca cgccagctcc agctcggcga
     1861 ggcgcgtctt ggccacctgc tccgggtcgt ggccgtcggc gcccggcttc acgtggccgg
     1921 gcccgtcgcc gtactcgccg ttggtgcagg tgaccacgac ggtccggatg ccctcggcgg
     1981 agtagcgggc cagcacgccc ccggtgctcg acgactcgtc gtcggggtgg gcgtgcacgg
     2041 ccatgagggt cagctctcga ggattgccca tggccgtagc ttgccccgtc gggccgcagt
     2101 tctcacgtcg cagttctcac gtcggagctc tcacgtcgga gctctcacgt cgagggcggg
     2161 ccgccgcccg ccccgcccgg atcgacgatc atcccggccc ccaccgtggc gtaggtggcc
     2221 tcgtcgacga ggatgaagct gccggtggtg cggttgcggg agtaggggtc gaacacgaga
     2281 ggcgtggcca cccgcagctc cacccggccg atctcgttga gggccaggcc gccggccgcg
     2341 ggctcgtggt gctccaggct gttgacgtcg agacgccatt cgatggactc caccacggcc
     2401 ttggtggtgc gcgtggtgtg cttcagcagg tacctgcccc gcgtcgtgag cgggcgctcc
     2461 gtcatccagc acacgtcggc gcgaagagac cgctccaccc tgggggccag cccgtcgccg
     2521 accacgacgt cgccccgcga cacgtcgatg tcgtcgctga gcctgatggt caccgacatg
     2581 ggcgggaagg cctcgccgat gggcccgtcc agggtgtcga tgccggccac ggtcgagcgc
     2641 tgcccggagg gcagcacgac caccgggtcg cccagccgga agacgccccc tgccaccgcg
     2701 ccggcgtagc cccggtagtc gtggtgctcg tcggagaagg gccggatgac gtactgcacc
     2761 gggaagcggg cgtcgcagag gttgcggtcc gacgcgatct ccacctcctc caggtggcgc
     2821 agcagcggcg ggccggcgta ccagggcatg ttgatcgacg gatcgacgac gttgtcgccc
     2881 cggagcgcgg agatcgggat gtaggtgacc tcgtgcacgt ccagcccgcc ggcgaaggcg
     2941 gcgaagtcgg cggcgatggc gtcgaacacc gtctcgtccc agcccaccag gtccatcttg
     3001 ttcacgcaca ccaccagatg ggggatgcgc aggaggctgg cgaggaaggc gtggcggcgg
     3061 ctctgctcca gcacgccgtt gcgggcgtcg acgagcacca ccgcgaggtc ggcggtcgac
     3121 gcgccggtga ccatgttgcg ggtgtactgc acgtggcccg gggtgtcggc gatgatgaac
     3181 ttgcgccggg gcgtggcgaa gtaccggtag gcgacgtcga tggtgatgcc ctgctcccgc
     3241 tcggcccgga ggccgtcggt gagcagcgcc aggttggtgt gctccaggcc gcgttggcgc
     3301 gacgagcgct ccaccgcctc gagctggtcc tcgaagatcg acttggcgtc gtacaggagc
     3361 cggccgatca gcgtggactt gccgtcgtcg accgagccgg cggtggcaaa ccgcaagagc
     3421 tccatcaacc cgctcgcaca ggtctagaaa tagccctcac gcttgcggtc ctccatggcc
     3481 gcctcggaga acttgtcgtc ggcccgggtc gcgccccgct cggtgatccg ggtggcggcg
     3541 atctcctcga tgatggcgtc gatgtccgag gcttcggaca gcaccgcgcc ggtgcagctc
     3601 atgtcgccga cggtgcggta acggacggtc gcgtcgagca cgtcctcttc cggcagcggg
     3661 gtcacgtgcg ggctcacggc gagcagcatc ccgtcccggg cgaagacctg ccggcggtgg
     3721 gcgaagtaga tggaaggcac ctccagccgc tcgcgggcga tgtactgcca cacgtcgagc
     3781 tcggtccagt tggagatcgg gaaggcccgg atgtgctcgc cccggcggat gcgcccgttg
     3841 tagagctccc agagctcggg ccgctggtgc ttggggtccc actggccgaa gtcgtcccgg
     3901 aaggagagca cccgctcctt ggcccgggcc ttctcctcgt cccggcgggc ccccccgaag
     3961 gcggcgtcga agccctgggc ctcgatggtg tcgaggaggg tcgtggtctg gagccggttg
     4021 cgggaggccc gcgggccgag ctcctccacc acccggccct ggtcgatgga gtcctggacc
     4081 tgcgccacga tcaagcgctc cccgagctcg gcgacgcgcc ggtcgcggaa ctccaggacc
     4141 tcggggaaat tgtggcccgt gtccacgtgc atcaccggga aggggaagcg ccccggccgg
     4201 aaggccttct cggccaggcg gagcagcacg atcgagtcct tgccgccgga gaagagcagc
     4261 accggccgct ccagctcggc ggccacctcg cgcatgatga agacggcctc ggcctcgagc
     4321 gcgtcgaggt gggtcagctc gaaaggcgac gggcacgggg gctccggcgc cgggcgcggg
     4381 gctggggcgg cggtgggggt cagggtcatg gtcatggaag gtccaggctc ctcagacgtg
     4441 cagtccgcac tcggtcttgt cgctgcctgc ccatcgtccg gcccggcggt cctcaccggg
     4501 ggcgacgggt cgggtgcagg gccagcagcc gatggagccg aagccccgct cggccagggg
     4561 gtgggtcaac agctggtgat cggagatgta gccggccatg tcctcgtcgg tccaggtggc
     4621 cagggggttg atcttgacca tggcgcgccg ggcgtcccac tccacgaccg gcgtggtggc
     4681 gcgggtcggg gcgtcgaccc gccgcaggcc ggtcacccac gccgccttgc cggcgaggcc
     4741 ccggttgagg ggctcgacct tgcggatccc gcagcagccg gccaggtcgc tctgccagag
     4801 gttggagcgc tccacggtcg gcctcatcac ggtgatgttg agcccgagct gggcccgaag
     4861 ctcctccatg taccacagcg tctcggcgaa gtggaactgg gtgtccagga agagcacctc
     4921 gatcccgggc acggccgccg ccgccatgtg cagcagcacc gggtcctcga aggacgaggc
     4981 cagcgcgagc gacgagccga aggcgtcgcc ggcccagcgc agcacgcggc cgggaggggc
     5041 cgtctcgaag gaggcggcca gcgctgggac gtcgtcgggg gcgatgagca cgtcggtcat
     5101 gacgccgcgc actccccttc gccgacgtcg gccacgtagg ggccctgctc gtcgaagtcg
     5161 atgtagaagt cgggccgctc ctccggggag gggaagacgt cgagctcctt gagcgacgcg
     5221 cccacggccg aggccccacc ggagcgggac agccagctct ggaaggcctc gcccggggtc
     5281 cgctcctctt cgtagcggcg gaccacgacc acggtcgcct cgccgacgcg cttggccggc
     5341 agccgaaggg ccttggcccc gaactcgatc cgggcgtcgc cgacgtggcc gccgagcagc
     5401 atctggtagc cgggggcgga gcgcccgtgg gctcgccgct cgacgcccat gaagccgatg
     5461 tcggagacgt ggtgctggcc gcagctgttg gtgcagccgg agatgttgat gcgcaccccg
     5521 ccgatgtcgg ccaggccggc ctcctccagc gaccgcccga tgtcgtcggc cagcccccgg
     5581 ctctgggtga cggccaggtt gcacgtgtcg gcgccagggc aggagaccac gtcgcgggcc
     5641 agctcggcgc ccggcgccgc catgcccgcg gcgtccagcc gggcgaacag gtcggggagc
     5701 tggagctcgg agaggccccg gaccacgaaa ttttgccggt tggtgacgcg cacgtcgaca
     5761 ccgaggtcgc gctggatggt ggcgacggcg cggaactgct ccgcggtcac gtcgcccagc
     5821 cggcaccagg cataggccga caccgtgccg c
//
LOCUS       DASWGG010000131         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1103487, whole genome shotgun sequence.
ACCESSION   DASWGG010000131 DASWGG010000000
VERSION     DASWGG010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103487"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VF954_02290"
     CDS             complement(<1..270)
                     /locus_tag="VF954_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9529939.1"
                     /translation="MGQDAGTIVVVEDDPNIADLVDMYLRREGFRVLQASDGRAGLAL
                     VDRDKPRLVILDIGLPGEMDGLDVCRRLRAVTGIPVLMLTARDGEI"
     gene            352..1158
                     /locus_tag="VF954_02295"
     CDS             352..1158
                     /locus_tag="VF954_02295"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX9529940.1"
                     /translation="MATYLDRILAAHRTAAQADDRPVGPRIEAAAAMAPARPFVQALR
                     ESPHLAVIAEIKRRSPSRGELAPGLDPAAMARAYASGGAVCLSVLTDEEFFGGTPADL
                     AAARAACALPTLRKDFTLSEGDVVDARLMGADAVLLIAAALDDAELRRLHGLATEVGL
                     AALVEVHDHAELERAAAVGAVLIGVNQRDLVTFEVDHARAEQVGAAIPAGVVRVAESG
                     IRDAADACRLAEAGFDAVLVGETLVRAIDPAAVVRALGVARLGQGARPCW"
     gene            1177..1833
                     /locus_tag="VF954_02300"
     CDS             1177..1833
                     /locus_tag="VF954_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX9529941.1"
                     /translation="MWVKICGTTSEQDALLAVAMGADALGFVFAPSPRQVSTAVASDI
                     VKRLPPEIVTVGVFRDEAPERVVDLVRTTGLRGAQLSGRESAEESKWVRRRLPFLIKG
                     FSAGSRAVADAAAYGADLILLDAPSPGSGEVFDWSMADAVPDGQRLVLAGGLTPENVA
                     GAIAQVNPWGVDVATGVEDGPGRKDARKLRAFIAAARAAAPAEYEATGEAPYDWRDDG
                     "
     gene            1830..>2157
                     /locus_tag="VF954_02305"
     CDS             1830..>2157
                     /locus_tag="VF954_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9529942.1"
                     /translation="MTTAMGEPSSEGRFGPFGGRFLPESLVPACEELEAAFRAAWADP
                     AFHAEYEEILRSYAGRPTPVTECHRLSDRLGVRVLLKREDLTHTGSHKINNVIGQALL
                     TLRMGKG"
BASE COUNT          263 a          797 c          802 g          295 t
ORIGIN      
        1 gatctcgccg tcccgggccg tcagcatcag cacgggtatg ccggtgacgg cccgcagccg
       61 gcggcacacg tcgaggccgt ccatctcccc cggcaggccg atgtcgagga tcaccagtcg
      121 cggcttgtcg cgatcgacga gcgccagccc ggcccgcccg tcgctcgcct ggagcacgcg
      181 gaacccctcc cgccgcaggt acatgtcgac caggtcggcg atgttgggat cgtcctcgac
      241 gaccacgatc gtgcccgcgt cctggcccat ccccccaggt tggcaccggc gggttagcga
      301 agtgtgaact cccctggacg gcgggcccgg ccaagtaggc tgcgtcggcc catggctacc
      361 tacctcgatc gcatcttggc cgcccaccgg acggcggcgc aggccgacga tcggccggtc
      421 ggcccgcgga tcgaagcggc cgcggccatg gcgccggcgc gtccgttcgt ccaggcgctg
      481 cgggagtcgc cccacctggc tgtcatcgcg gagatcaagc gccgatcgcc ttccagaggg
      541 gagctggcgc cggggctgga cccggcggcc atggcccggg cctacgcaag cggaggggcg
      601 gtgtgcctct cggtcctcac cgacgaggag ttcttcgggg ggacgccggc tgacctggct
      661 gccgcccggg cggcgtgcgc cttgcccacc ctgcggaagg acttcaccct cagcgagggc
      721 gacgtggtgg atgcccggct catgggggcc gacgccgtgc tgctgatcgc cgccgccctc
      781 gacgacgccg agctccggcg cctgcacggc ctggcgacgg aggtggggct ggcggccctc
      841 gtcgaggtcc acgaccacgc cgagctggag cgcgccgcgg cggtgggcgc cgttctcatc
      901 ggcgtgaacc agcgcgacct ggtcaccttc gaggtcgacc acgcccgggc cgagcaggtc
      961 ggtgcggcca tcccggccgg cgtggtgcgc gtcgcggagt cgggcatacg cgacgcggcc
     1021 gacgcctgcc gcctggccga ggccggcttc gacgccgtgc tcgtcgggga gacgctggtg
     1081 cgggccatcg accccgctgc cgtggtgcgt gccctcggcg tcgcccgcct ggggcagggg
     1141 gcccggccgt gttggtgacg acgtgcgggt gacgacgtgt gggtgaagat ctgcgggacc
     1201 acgagcgagc aggacgccct gctcgcggtc gccatgggcg ccgacgccct cggcttcgtc
     1261 ttcgctccct cgccccgcca ggtcagcacc gcggtcgcct cggacatcgt caagcgcctc
     1321 ccgcccgaga tcgtgaccgt aggggtgttc cgggacgagg cacccgagcg ggtggtggac
     1381 ctggtccgca cgacgggcct gcggggcgcg cagctctccg gtcgggagtc cgcggaggag
     1441 tcgaagtggg tccgccgccg cctcccgttc ctcatcaagg gtttctcggc cggcagccgc
     1501 gcggtcgccg acgccgccgc ctacggcgcc gatctgatcc tgctcgacgc cccctcgccg
     1561 ggatcgggag aggtcttcga ctggtccatg gccgacgccg tgcccgacgg ccagcgcctc
     1621 gtgctcgccg gcggcctcac gcctgagaac gtggccgggg cgatcgccca ggtcaacccg
     1681 tggggcgtcg acgtggcgac tggcgtggag gacgggcccg gccgcaagga cgcccgcaag
     1741 ctccgcgcct tcatcgccgc ggcgcgggcg gccgcgcccg ccgagtacga agccactggc
     1801 gaggcgccgt acgactggag ggacgacggg tgacgaccgc catgggtgag ccctcgtcgg
     1861 aggggcggtt cgggccgttc ggcgggcggt tcctccccga gtcgctcgtg ccggcgtgcg
     1921 aggagctgga ggccgccttc cgggcggcgt gggccgatcc ggcgttccac gccgagtacg
     1981 aggagatcct gcgctcgtac gcgggccggc ctacgccggt gaccgagtgc caccgcctct
     2041 ccgaccggct cggcgtccgg gtcctgctca agcgggagga cctcacccac acgggctccc
     2101 acaagatcaa caacgtcatc ggccaggccc tcctcaccct ccgcatgggc aaggggc
//
LOCUS       DASWGG010000132         3338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1748166, whole genome shotgun sequence.
ACCESSION   DASWGG010000132 DASWGG010000000
VERSION     DASWGG010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748166"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="VF954_02310"
     CDS             <1..1408
                     /locus_tag="VF954_02310"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX9529943.1"
                     /translation="DPARAMQTEIQIITSAPVRQALAAKLGEAPRISASPVGLTDVLS
                     VRAESTDPARAATVANAYANAYIDFKRTEGVNDLLAAAAQIQSKVSDLQHQIDDLNLR
                     ITSAAPLAQAGVRSEVGPQIDTLIQQQGAYKQQLSNLQVQANLDTGGAQLVTPATVTT
                     VPVRPRPKRDAAIAVVVGLILGVGLAFLLEYLDDTLKSKEDVERVVRDVPVLGLVPAV
                     PGWKPKDDPVVVALTDPHAPAAESYRALRTALQFMALDRPMRTLQVTSPTAQEGKTTT
                     LASLGVALAQSGQRVVVVGCDLRRPRIHDFYRLRNEIGFTSVLLGDVPLSAALQPVPG
                     VDNLRLLASGPVPPNPAELLSGRRTVEVLTALQAEADIVLVDCPPVLPVTDAAVLSGR
                     VDATLLVLWAGETTRKELSRAAELLGHVDAPIVGVVLNGIKSESGYGYAYNYKYYGPG
                     DRARKSRWPRRAKEPAPS"
     gene            complement(1417..>3338)
                     /locus_tag="VF954_02315"
     CDS             complement(1417..>3338)
                     /locus_tag="VF954_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEX9529944.1"
                     /translation="WPAASRGTAVLGALAVVLVALPGLGALRARARRRVASVVGAAAA
                     VAGLAGVAGLFALALARPHADLGVQTARTAIDRAASGDVSGARQQIGDTRRAFEAASR
                     YTDAWWAAPGLVLPGAGHDFRAVRDAVRAGRRLAAAGTDLSASTDPSQLRWSGGRLPV
                     DQLRRLEPIAARAVSATAQARADLRSARSPWVLSLLGRRLTPLASRVGRAQSDAEHLM
                     TALQIGPGLVGGDGPRRYFLAVETPAEARATGGFIGNYGEITADGGALHLDALGRTMD
                     LNTRGDPAVRTLAAPADYVTRYGRFDPALNWQNVTMSPDFPTVATVIEGLYPQSGGRP
                     VDGVVAVDPYGLADLLRATGPVSVPSWPVPLTADNAAHVLLFDQYVSFANSPLRVDFL
                     GEVTRAVWDKFVTTPISLADLAKDVGPAVAGRHLLAASTDGAEQAGLERLDAAGRMTP
                     VTDDYVQLVTQNASGNKIDWFQATRLDYRPRVAGDDLTAQAVITLSDGAPAAGLPPYI
                     IGNALNPPAPPGSERVYVSLYTPWALKGATLDGRQLLMESQVEAGRHVYSAFVDLPSG
                     GSRTITVQLAGRRAGPRAYHLDVQRQPAVAVTRVRTAVVGDAAWRCVPPPAAGAGGAV
                     AGDSPLDFAVDCTTA"
BASE COUNT          465 a         1249 c         1228 g          396 t
ORIGIN      
        1 cgacccggct cgcgccatgc agaccgagat ccagatcatc acgagcgcgc ccgtgcggca
       61 ggccttggcc gccaagctgg gggaggcgcc caggatcagc gcctccccgg tcggcctgac
      121 cgatgtgctc agcgtccggg ccgagagcac cgacccggcc cgggcggcca ccgtcgccaa
      181 cgcctacgcc aacgcctaca tcgacttcaa gcgcaccgaa ggcgtcaacg acctgctggc
      241 ggcggccgcc cagatccagt ccaaggtgtc agatctccag caccagatcg acgacctcaa
      301 cctcaggatc acctcggcgg ccccgctcgc ccaagccggg gtgcgctccg aggtcggccc
      361 gcagatcgac accctgatcc agcagcaggg tgcctacaag cagcagctga gcaacctcca
      421 ggtccaggcc aacctggaca cgggcggcgc ccagctggtg acgccggcga cggtgacgac
      481 ggtccccgtc cggccccggc ccaaacggga cgcggccatc gcggtcgtgg tcgggctcat
      541 cctgggcgtc gggttggcct tcctgctcga gtacctcgac gacaccctca agtccaagga
      601 ggacgtggag cgggtcgtgc gggacgtgcc cgtgctgggc ctggtccccg ccgtccccgg
      661 atggaagccc aaggacgacc cggtggtggt cgctctgacc gacccccacg cacccgcggc
      721 cgagtcgtac cgggcgctgc ggacggccct gcagttcatg gccctcgacc ggcccatgcg
      781 gacgctgcag gtcaccagcc cgaccgcgca ggaaggcaag accacgacgc tcgccagcct
      841 gggagtggcc ctcgcccagt ccggccagcg cgtcgtcgtc gtgggctgcg acctgcgccg
      901 gccccggatc cacgatttct accggctccg caacgagatc gggttcacgt cggtcctgtt
      961 gggcgacgtc cccctttccg ccgccctgca gccggtgccc ggggtcgaca acctccggct
     1021 gctcgcctcg ggcccggtgc cgccgaaccc ggccgagctg ctgtcgggcc ggcgcaccgt
     1081 cgaggtcctg accgcgctgc aggccgaagc cgacatcgtc ctcgtcgact gcccgccggt
     1141 cctccccgtc accgacgcgg ccgtgctgtc cggtcgcgtg gacgccaccc tgctcgtgct
     1201 gtgggcgggc gagacgacgc gcaaagagct cagccgggcc gccgagctgc tgggccacgt
     1261 ggacgccccg atcgtcggcg tggtgctcaa cggcatcaag agcgagtcgg gctacgggta
     1321 cgcctacaac tacaagtact acggcccggg tgaccgcgcc cgcaagagcc ggtggccgcg
     1381 ccgggccaag gagcccgctc ccagctgacc cgccggctag gccgtcgtgc agtcgacggc
     1441 gaagtcgagc ggcgagtccc ccgcgacggc cccgccggcc ccagccgcag gcggcggcac
     1501 gcaacgccat gccgcgtcgc cgacgaccgc cgtgcgcacg cgggtcacgg ccacggccgg
     1561 ctggcgctgc acgtcgaggt ggtacgccct gggacccgcc cggcggcccg ccagctgcac
     1621 cgtgatcgtc cgcgaccccc ccgagggcag atcgacgaac gcggagtaga cgtggcggcc
     1681 cgcctcgacc tgcgactcca tcagcagctg ccgcccgtcg agggtcgcgc ccttcagggc
     1741 ccagggcgtg tacaaggaaa cgtagacgcg ttcgctgccg ggcggtgcgg gcggattgag
     1801 ggcgttgccg atgatgtacg gcggcaggcc ggcggccggc gcgccgtcgg agagggtgat
     1861 caccgcctgg gcggtcaggt cgtcgccggc gacgcggggc cggtagtcga gacgcgtcgc
     1921 ctggaaccag tcgatcttgt tgcccgaggc gttctgggtc actagctgga cgtagtcgtc
     1981 ggtgacgggc gtcatcctcc cggccgcgtc caggcgctcg agcccggcct gctcggcgcc
     2041 gtcggtgctg gccgccagga ggtgccggcc ggccacggcc gggccgacgt ccttggccag
     2101 gtcggcgagg gagatcggcg tggtgacgaa cttgtcccac accgcccggg tgacctcgcc
     2161 caggaagtcc acccgcagcg gagagttggc gaagctgacg tactggtcga acagcagcac
     2221 gtgggccgcg ttgtccgccg tcagtggcac gggccatgag ggcacggaca ccgggcccgt
     2281 ggcgcgcagc aggtcggcga gaccgtaggg gtccacggcg accacgccgt cgaccggccg
     2341 gccgccggac tgggggtaga ggccctcgat gaccgtggcc acggtgggga agtcgggcga
     2401 catggtgacg ttctgccagt tgagcgcggg gtcgaagcgg ccgtagcggg tgacgtagtc
     2461 cgcaggggcc gcgagcgtgc gcacggcggg gtcgccccgg gtgttgaggt ccatcgtccg
     2521 ccccagggcg tcgaggtgca gggcgccacc gtcggccgtg atctcgccgt agttgcctat
     2581 gaacccgccc gtcgcccgcg cctcggccgg cgtctccacc gccaggaagt accggcgcgg
     2641 tccgtcaccg ccgaccaggc cgggcccgat ctgaagggcg gtcatgagat gctcagcgtc
     2701 ggactgggcc cggcccaccc ggctggcgag cggggtcaga cgccgcccga ggagggacag
     2761 gacccacggc gaccgggccg agcggagatc ggctcgtgcc tgggccgtgg ccgagacggc
     2821 ccgggccgcg atgggctcga gccggcggag ctggtcgacg gggaggcgcc caccggacca
     2881 tcgcagctgg ctcgggtccg tagacgcgga caggtcggtg ccggctgcgg ccagccgccg
     2941 gccggcccgt accgcgtctc gcacggcccg gaagtcgtga cccgcgcccg gcaggaccag
     3001 gcccggtgcg gcccaccagg cgtcggtgta gcgggaggct gcctcgaagg cgcgccgggt
     3061 atcgccgatc tgctgccgcg ccccggacac gtcgccgctg gcggcccggt cgatggccgt
     3121 ccgggccgtc tgcacgccga gatcggcgtg cggccgggcc agggcgaggg cgaacaggcc
     3181 cgccaccccg gccagtccgg cgacggccgc cgccgcgccc accacggacg ccacccgccg
     3241 gcgcgcccgg gcccggagag cccccaggcc gggcagcgcg accaggacca cggcgagcgc
     3301 gcccagaacg gcggtgcccc gcgacgccgc cggccagc
//
LOCUS       DASWGG010000133         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_65801, whole genome shotgun sequence.
ACCESSION   DASWGG010000133 DASWGG010000000
VERSION     DASWGG010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65801"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="cofD"
                     /locus_tag="VF954_02320"
     CDS             complement(<1..842)
                     /gene="cofD"
                     /locus_tag="VF954_02320"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX9529945.1"
                     /translation="MIAALAGGVGAARFLRGLLGVVPPADVTAIVNTGDDLVLHGLHV
                     SPDLDTVTYTLAGAINPDLGWGLAGETWAAMTALERYRGQTWFRLGDRDLATHLYRTQ
                     RLAEGAALSTVTAEIASAWGLHLRVLPVTDDELTTRVTVAGEGEIGFQEYFVGRHHDV
                     SIEGVRFEGADRARPGPGVLAAIEDAGTVVICPSNPIVSIAPLLAVPGVREAVEAARR
                     RTVAVSPIVAGAALKGPADRMLSELGHESSVVGVARLYAPLAAALVVDTADAGLAGEV
                     EAEG"
     gene            878..1996
                     /locus_tag="VF954_02325"
     CDS             878..1996
                     /locus_tag="VF954_02325"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9529946.1"
                     /translation="MPLSPVPLRVALDATPLLGARTGVGVFTDCALQALAREPGLAVA
                     AFAVSWRRRQGIRPLLPAGCRMVERPMPARPLHWAWRRFPGPPVEWWTGPQDVVHGTN
                     FVVPPSRRAARVVTVHDLTTLRFPELCDDATLRFPALVRRAIAEGAWVHTPSSYVASE
                     VVELLGADPARVRAIHHGIPAIPAPAGPGPAGPYILALGTVEPRKDYPLLVAAFDRIA
                     GDRPQLRLVIAGPDGWGAGALGRALAAARFTDRVTRVGWVGERERARLFHGAAVFAFP
                     SVYEGFGLPPLEAMAAGVPVVASDAGALREVVGDGGWLVPQGDVDSLARALATVVDDA
                     ATAAALVERGRARAGAFSWERCARALAGLYADARDAKG"
     gene            2001..>2326
                     /locus_tag="VF954_02330"
     CDS             2001..>2326
                     /locus_tag="VF954_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529947.1"
                     /translation="MEILVVAEQLRRAVPGGIGTYTSGLFQGLAGLTGAGEPDVVLTL
                     LASRPPAGGDPLCRFGFPVRTVALPGPLMTRAWDRDRLGVPPGFAAVHAVSLASPPPG
                     STPLTV"
BASE COUNT          249 a          894 c          886 g          297 t
ORIGIN      
        1 atcccctcgg cctcgacctc gccggccagg ccggcgtcgg cggtgtcgac gaccagggcg
       61 gcggcgagcg gcgcgtacag ccgggccacc ccgacgaccg acgactcgtg gcccagctcg
      121 ctcagcatcc ggtcggccgg ccccttgagg gcggcgcccg ccacgatcgg ggacaccgcc
      181 acggtccgcc ggcgcgccgc ctccacggcc tcccgcacgc cgggcacagc gaggagcggg
      241 gcgatggaca cgatggggtt cgacgggcag atcaccaccg tgccggcgtc ctcgatggcg
      301 gcgagcacgc cgggcccggg ccgggcccgg tccgcaccct cgaagcgcac gccctcgatg
      361 ctcacgtcgt gatgcctgcc cacgaagtac tcctggaagc cgatctcgcc ctcgcctgcg
      421 acggtgacgc gcgtggtcag ctcgtcgtcg gtcacgggca ggacgcgcag gtgcagcccc
      481 cacgccgacg cgatctcggc ggtcacggtg gacagggcgg cgccctcggc gagccgctgg
      541 gtgcggtaga ggtgggtggc gaggtcccgg tccccgaggc ggaaccaggt ctggccgcgg
      601 tagcgctcga gcgcggtcat ggcggcccac gtctcgccgg ccagccccca gcccaggtcg
      661 gggttgatgg ccccggccag cgtataggtg acggtgtcga ggtcggggct gacgtggagg
      721 ccgtggagga ccagatcgtc gccggtgttg acgatggcgg tgacatcggc cggggggacg
      781 acgccgagca ggccgcgcag gaaacgggcc gcgccgacgc ccccggcgag ggcggcgatc
      841 acccaaccac ggtagtgcac cgggccagcc tcgcccgatg ccgctgagcc ccgtgccctt
      901 gagggtggcc ctggacgcca cgccgctgct cggggcccgc accggggtgg gcgtgttcac
      961 cgactgcgcc ctgcaggccc tggcccggga gcccggcctg gcggtggcgg cgttcgcggt
     1021 gagctggcgc cgccggcagg gcatccggcc cctcctgccc gccggctgcc gcatggtcga
     1081 gcggcccatg ccggcccggc cgctgcactg ggcctggcgg cgcttccccg gcccgccggt
     1141 cgagtggtgg accggccccc aggacgtggt gcacggcacc aacttcgtgg tcccccccag
     1201 ccggcgggcg gcgcgcgtgg tgaccgtcca cgacctcacg accctgcgct ttcccgagct
     1261 ctgcgacgac gccacgctcc ggttccccgc cctggtgcgc cgggcgatag ccgagggggc
     1321 gtgggtccac accccttcca gctacgtggc gtccgaggtg gtcgagctgc tgggcgccga
     1381 tccggcgagg gtccgcgcca tccaccacgg catcccggcg atccccgcgc cggccggccc
     1441 cggaccggcg ggcccgtaca tcctggccct gggcaccgtc gagccccgca aggactatcc
     1501 cctcctggtg gccgcattcg accggatcgc gggcgaccgc ccccagctgc ggctcgtcat
     1561 cgccgggccg gacgggtggg gcgcgggcgc gctgggccgg gccctggccg cggcccggtt
     1621 caccgaccgg gtcacccggg tcggatgggt cggggaaagg gagcgcgctc ggctgttcca
     1681 cggggcagcc gtgtttgcgt tcccctcggt gtacgagggc ttcgggctcc cgcccctcga
     1741 ggccatggcg gccggtgtgc cagtggtggc gagcgatgcg ggcgccctcc gcgaggtcgt
     1801 cggcgacggc ggttggctgg tgccccaggg cgacgtcgac tcgctcgctc gcgccctggc
     1861 cacggtcgtc gacgacgccg cgaccgctgc cgccctggtc gagcggggcc gggcccgcgc
     1921 cggcgccttc agctgggagc ggtgcgcccg cgccctggcc gggctgtacg ccgacgcacg
     1981 ggacgcgaaa gggtagggcg gtggagatcc tcgtggtcgc cgagcagctg cggcgggccg
     2041 tgcccggtgg catcggcacc tacaccagcg gcctgttcca gggcctcgcc ggcctcaccg
     2101 gtgccggtga gcccgatgtg gtgctgaccc tgctcgccag ccggccgccc gcgggcggcg
     2161 acccgctgtg ccggttcggc ttccccgtgc gaaccgtcgc cctcccaggc cctctcatga
     2221 cccgggcgtg ggaccgcgac cggctgggcg tgccgccggg cttcgccgcg gtccacgccg
     2281 tctcgctggc ctcgccgccc ccggggagca ctccgctcac ggtctt
//
LOCUS       DASWGG010000134         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3039326, whole genome shotgun sequence.
ACCESSION   DASWGG010000134 DASWGG010000000
VERSION     DASWGG010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3039326"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VF954_02335"
     CDS             complement(<1..577)
                     /locus_tag="VF954_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529948.1"
                     /translation="MIGTKVPALALLAAMAMTAVACAAKDNGGAFGAPPSSTGPAPPT
                     DPAAARLLPATFQASHQLKAGVDPSHPPLAFLQGSSVQGLDEDLIQAIGAKLGLRITL
                     VQGVPGDQVDVLLPGPPSRVATAPPAAQVVPFLRIGLATVVKAGTSSCAGPVAVVPDV
                     PFPACLGPHVPVATPGDAVAALQQGRVNALVT"
     gene            complement(574..1290)
                     /locus_tag="VF954_02340"
     CDS             complement(574..1290)
                     /locus_tag="VF954_02340"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX9529949.1"
                     /translation="MAQGVGTRGTAKRAKPVTRATVPSLAVERQLWTDGHEVVVGVDE
                     VGRGAWAGPLSVGAAVLPQDRRVYRVRDSKLLTEREREQMYDRVAGWCTAWAVGHATY
                     EECDRLGMSEAQRLAARRAIDGLGVTPDQVLIDGNWDFVGTGNTRRIVKGDAHCLSIA
                     AASILAKVTRDRLMRAEAPHFPSFDFELNKGYPCPRHKCALRAWGPTSIHRRTWVFMD
                     HLVWGVHPVPAVQGELVLES"
     gene            complement(1297..>1572)
                     /locus_tag="VF954_02345"
     CDS             complement(1297..>1572)
                     /locus_tag="VF954_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529950.1"
                     /translation="EHWWGQPVEIHDAPTHHGRLSAALRWHGDRPALLWQLVPAPWRD
                     AEPGRLRAPALDPNWASSEPRGDVLLAPARGASGQRHAARPPGGPLA"
BASE COUNT          214 a          580 c          577 g          201 t
ORIGIN      
        1 cggtcacgag cgcgttcacc cggccctgct gcaaggccgc gaccgcatcg cccggcgtgg
       61 ccacggggac atgcgggccg aggcacgccg ggaacgggac gtcggggacg acggccaccg
      121 ggccggcgca cgaggaggtg ccggccttca ccaccgtcgc caggccgatc cggagaaagg
      181 gcacgacctg tgccgcaggg ggcgccgtgg ccactcgcga cggtggtccc ggcaggagca
      241 cgtcgacctg atcgccgggc accccctgca cgagggtgat cctcagcccc agcttggccc
      301 cgatggcctg gatcaggtcc tcgtccaacc cctgcacgct gctcccctga aggaacgcaa
      361 gcggcgggtg gctcggatcg acccctgcct tcagctggtg gctggcctgg aaggtggcgg
      421 gcaggaggcg ggccgcggcc gggtcggtcg gcggagcagg gccggtgctg ctgggcgggg
      481 cgccgaacgc gccaccgttg tccttcgccg cgcacgcgac cgcggtcatg gccatggcgg
      541 ccagcagggc gagagccggg acctttgtcc cgatcaagac tcgaggacca gctcgccctg
      601 caccgccggc accgggtgca cgccccatac cagatggtcc atgaagaccc aggtccggcg
      661 gtggatcgag gtcgggcccc acgcccgcag ggcgcatttg tgccgcgggc acggatagcc
      721 cttgttgagc tcgaagtcga agctggggaa gtgtggcgcc tccgcccgca tcaggcggtc
      781 ccgcgtcacc ttggccagga tcgacgccgc cgcgatggac aggcagtggg cgtcgccctt
      841 gacgatccgg cgggtgttgc cggtgccgac gaagtcccag ttcccgtcga tgagcacctg
      901 atccggcgtc acgccgaggc cgtcgatcgc gcggcgggcc gccagccgct gggcctccga
      961 catgccgagg cggtcgcact cctcgtaggt ggcgtgaccc accgcccacg ccgtgcacca
     1021 gccggcgacg cggtcgtaca tctgctcgcg ctcccgctcg gtgagcagct tggagtccct
     1081 gacccggtac acccggcggt cctgcggcag gacagccgcg ccgacggaga ggggaccggc
     1141 ccaggcgccc cgtccgacct cgtcgacgcc gaccaccacc tcgtggccgt cggtccacag
     1201 ctgccgctcg accgcgagcg atgggaccgt agcccgcgtc accggcttgg cccgcttggc
     1261 cgttcccctc gtaccgaccc cctgcgccat gggagctcac gctagcgggc cgcccggcgg
     1321 gcgggcggca tggcgttgcc cggaagcgcc ccgggcggga gcgagcagga cgtcaccgcg
     1381 tggctccgag ctggcccaat tcgggtccag ggccggggct cgcagccggc ccggctcggc
     1441 gtcgcgccat ggcgccggca ccagctgcca caacagggcg ggacgatctc cgtgccatcg
     1501 cagcgcggcc gacagcctgc cgtggtgggt gggagcgtcg tggatctcga cgggttgacc
     1561 ccaccagtgc tc
//
LOCUS       DASWGG010000135         6124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1423023, whole genome shotgun sequence.
ACCESSION   DASWGG010000135 DASWGG010000000
VERSION     DASWGG010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423023"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VF954_02350"
     CDS             complement(<1..230)
                     /locus_tag="VF954_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529951.1"
                     /translation="MQLERRGRLERVRPGRSLVAALAVAAALLGSAGAAMAATSGASD
                     ESAFLAATNSARAGHGLAWLASAGDLVALARQ"
     gene            423..1034
                     /locus_tag="VF954_02355"
     CDS             423..1034
                     /locus_tag="VF954_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529952.1"
                     /translation="MSTRPLVELVGPVELEVPGRVPVFVDDSGRRRRYTAWFGRGLAV
                     LVGAYVLLLAASLARAPWVPKVSLPIVGNVLKPPGPQALALGATATTTQAPALAPGAP
                     GAAASAPAGGVSPTGAASGGAGALRPGPTSPGVASTPTATTLPASATSTTVALPGSSG
                     TAPGRTSTTVGSATTTRPSGAGTHNPHDTTTTAPGKGTGTSVP"
     gene            1031..3157
                     /locus_tag="VF954_02360"
     CDS             1031..3157
                     /locus_tag="VF954_02360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9529953.1"
                     /translation="MSTPSRGALRRRRWRPPPHWLAFGAVLGGIVLALLVDGIAHNRV
                     GAASTVARSGRPLGGLGGVGPIADFSSSTIRSAQPPANQVALTFDDGPDPRWTPQVLA
                     LLGRYRVPATFFVVGSKVTTHPGLVRAEVAAGDEVGSHTFTHAELGAVSGTRANLELS
                     LTQVALAGAIGRSSSLLRLPYSSQPQALTASGYRAARAAGRYGYLVVFADRVTEDWRR
                     PGAAVIAARAIPPPGQGAVILLHDGGGDRSQTLAALAQLVPALQARGVQLVTISQLLG
                     RSAQASLAPVGTVQHAQGLALLWSVRSAHWLVVAVFALIVPLTLLVLARTVAVVVLAG
                     RQARASGRRPMRPPPVEELPPVSAVVPAYNEEVGIAATVRSLLGSGLGDLEVVVVDDG
                     STDGTAAAVQAVADPRVRLIRQANAGKPAALNAGVAEATHDILVLLDGDTVFERDTLA
                     WLVGPLRHPGVGAVCGNAKVGNRRRLLGRWQHVEYVLVHNLDRRAQDRLHCITTVPGA
                     AGAFRRQALAAVGGVSDDTLAEDTDLTMALARAGWRVVYEERARAHTEAPAGLNDLWR
                     QRYRWCYGSLQAMWKHRGAVGEGGRLGRVALPWLALFGVLLPLLSPVLDLVAVYALVF
                     GRAREVLAIWLAYNVIQVALAAYALHLDGERTTDAWVVPLQQVVYRQLMYLVVIQSAL
                     SALAGTRLRWHKLARTGEVIVPPLAP"
     gene            3301..3639
                     /locus_tag="VF954_02365"
     CDS             3301..3639
                     /locus_tag="VF954_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9529954.1"
                     /translation="MTSNENDETNGAFWVSTSEMAGVSTIVIQGELDLATAPLVTAKL
                     EEAFSIPGSLVIDVTYMGFMDSSGLGILAKAFRRARTDGRSLALRNPSPPIRRTLEIA
                     GLDFLIERRD"
     gene            3737..4327
                     /locus_tag="VF954_02370"
     CDS             3737..4327
                     /locus_tag="VF954_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9529955.1"
                     /translation="MTIGAEFDTLLAAAQAGGEWAFAALYRDLNPKLLRYLSAHAPGA
                     AEDVAAETWLGVARGLRSFTGGEGAFRSWFFTIAHRQLVQLWRDRGRRPSSPADPESF
                     ADLAAPDDPEAAGVAIVSAAEAARTIRAALSRDQADVVLLRVLAGLDADQVAEVLGKR
                     PATVRVLQHRALRKLEGQFSPEPVTRRPPGAIAPVT"
     gene            4324..5148
                     /locus_tag="VF954_02375"
     CDS             4324..5148
                     /locus_tag="VF954_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529956.1"
                     /translation="MTRHHLDDIEAGRLLAGRLAPEDAPPGYAGLATLVRAAAGPATV
                     AELAGEATVVAAALTVLGAAGAGDARSRRSSRWVRALTAKIAAVAVVTVAGATAAAAA
                     TGSLPAPAQTAVSDAASHLGISVPEADHHGPAASTGAPTGHRGAPTPKVAGSGQDANT
                     FGQCTAFLAGTAAGKDASTAFKDFIAEHDGTVASTTSFCNTFLATRTAGHGQAGPGTQ
                     PSTTDDAGGRSTTSDHPGRPADKPATTSTTHPGDGGGHGAAPAPSSAAPRTGSSHP"
     gene            complement(5208..5798)
                     /locus_tag="VF954_02380"
     CDS             complement(5208..5798)
                     /locus_tag="VF954_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX9529957.1"
                     /translation="MRGTGAGGPVVALVSSAGGLDALARVLSPLPPGYPAAVIALQHL
                     DPIRASGLAAILAHRAQLPVRFAEQGTELTPGVVVVAPPGLHTLVLPDRTLRLIVSGA
                     APPSRPSADLLLTTLAISVGAAAIAVVLSGGGHDGATGATAVHDLGGTVIAADEHSSE
                     QFSMPHATIGRADAVDHVAGVDQIAGLLQSLVVARS"
     gene            complement(5835..>6124)
                     /locus_tag="VF954_02385"
     CDS             complement(5835..>6124)
                     /locus_tag="VF954_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX9529958.1"
                     /translation="GMNLVAKEAVVVNERDGVLLLSENAGADHELGHFALRVHPFDIE
                     QQAAAMWDALTMDARERRDRREACAAVVRRNGLARWVRAQLEDARRNGAAG"
BASE COUNT          733 a         2429 c         2188 g          774 t
ORIGIN      
        1 tgctgccggg ccagggccac caggtcaccg gcggaggcca gccacgccag gccgtggccg
       61 gcccgggccg agttggtggc ggcgaggaag gcggactcgt cggaggcgcc gctcgtggcc
      121 gccatggcgg cgccggccga gcccagcagg gcggcggcga cagccagcgc ggccacgagg
      181 gaccggcccg gcctgacccg ttcgagcctc ccccgtcgtt cgagctgcat gctatcgtcc
      241 ccctcgtcac tttcgactag gactgttatc gctcgaacgg gcctggttct atacccctct
      301 gctgggctat ttgcgaaatg gcacatgtgg gctagtcaac aagttcgacg cccccgcccc
      361 cgtgtcctcg ccgccggcgc ccgtaacgac gcggcatcct ggagcgatgg cctggaccgg
      421 acatgagcac ccgacccctc gttgagctcg tcgggccggt cgagctcgag gtacccgggc
      481 gggtcccggt cttcgtggac gactccggcc ggcggcgccg gtacaccgcc tggttcgggc
      541 gcggcctcgc cgtgctcgtc ggcgcctacg tgctgctgct cgcggccagc ctggcccggg
      601 ccccgtgggt cccgaaggtg tcgctgccga tcgtcggcaa cgtcttgaag ccgcccggac
      661 cgcaggccct ggccctggga gccaccgcca ccaccaccca ggcgcccgcg ctggccccgg
      721 gcgcgccggg cgcggccgca tcggccccgg ccggcggcgt ctcgcccacc ggcgcggcgt
      781 cgggcggcgc cggcgcgctt cgcccgggtc ccacctcgcc aggcgtcgcg tcgactccga
      841 ccgcgacgac cctgccggca tccgccacct cgacgacggt cgccctgccc ggctcgtccg
      901 gcacggcgcc cggccggacc tcgacgaccg tgggctcggc caccaccacc cggccgtcgg
      961 gcgccggtac ccacaacccc cacgacacca ccaccaccgc gccgggcaag ggcaccggga
     1021 catccgttcc gtgagcacgc ccagccgggg cgccctgcgg cgcaggcggt ggcggccgcc
     1081 gccgcactgg ctggccttcg gcgccgtcct gggcgggatc gtgctcgccc tcctggtcga
     1141 cggcatcgcc cacaaccgcg tgggcgcggc gtcgaccgtg gcccggtcgg gccggcccct
     1201 cggcgggctc ggcggggtgg gcccgatcgc cgacttctcc tcctcgacca tccgcagcgc
     1261 ccagccgccg gccaaccagg tggcgctgac cttcgacgac gggcccgatc cgcggtggac
     1321 gccccaggtc ctggccctgc tgggtcgcta ccgcgtcccc gccacgttct tcgtggtcgg
     1381 ctccaaggtg accacccatc ccggcctggt gcgggccgag gtggccgccg gcgacgaggt
     1441 cgggtcccac accttcaccc acgccgagct gggtgcggtg tccggcaccc gggccaacct
     1501 cgagctgtcg ttgacgcagg tcgccctggc cggcgccatc gggcggtcca gctcgctgct
     1561 ccggctcccc tactccagcc agccgcaggc gctgacggcg tcggggtacc gggcggcgcg
     1621 ggcggccggc cgctacggct acctcgtcgt gttcgccgac cgggtgaccg aggactggcg
     1681 ccggcccggc gcggccgtca tcgcggcccg ggccatcccg ccgcccgggc agggcgccgt
     1741 catcctgctg cacgacggcg gcggggaccg gtcccagacg ctggccgccc tggcccagct
     1801 ggtgccggcg ctccaggccc ggggcgtgca gctcgtgacc atctcgcagc tgctcggtcg
     1861 ctccgcccag gcctcgctgg caccggtcgg caccgtgcag cacgcccagg gcctggccct
     1921 gctctggtcg gtgcggtcgg cgcactggct ggtggtcgcg gtcttcgccc tgatcgttcc
     1981 gctgaccctg ctggtcctgg cccgcaccgt ggccgtcgtc gtgctggcgg gccggcaggc
     2041 ccgggccagc ggtcggcggc ccatgcggcc accgcccgtc gaggagctgc cgccggtcag
     2101 cgccgtcgtg cccgcctaca acgaggaggt gggcatcgcg gccaccgtgc gatcgctgct
     2161 cggctccggc ctcggcgacc tcgaggtcgt cgtggtcgac gacggctcca cggacgggac
     2221 cgcggccgcc gtgcaggccg tggccgatcc ccgggtgcgg ctcatccgcc aggccaacgc
     2281 gggcaagccg gccgccttga acgcgggcgt ggccgaagcc acccacgaca tcctcgtgct
     2341 cctagacggc gacacggtgt tcgagcgcga caccctcgcc tggctggtgg gacctctgcg
     2401 ccatcccggc gtcggcgccg tctgcggcaa cgccaaggtg ggcaaccggc ggcgcctgct
     2461 cgggaggtgg cagcacgtcg agtacgtcct ggtccacaac ctcgaccgcc gggcccagga
     2521 ccggctgcac tgcatcacga cggtcccggg cgcggcggga gcctttcgcc gccaggctct
     2581 cgccgcggtc gggggcgtga gcgacgacac cttggccgag gacacggacc tgaccatggc
     2641 gctggcccgg gcgggctggc gcgtggtcta cgaggagcgg gcccgggccc acaccgaggc
     2701 gccggcgggc ctgaacgacc tgtggcgcca gcgctaccgc tggtgctacg ggagcctcca
     2761 ggccatgtgg aagcaccggg gggccgtcgg cgagggcggg cggctcggcc gcgtcgcgct
     2821 gccctggctg gccctgttcg gggtgctgct ccccctgctg tcccccgtgc tcgacctggt
     2881 cgccgtgtac gccctggtct tcggccgggc gcgggaggtg ctggccatct ggctggccta
     2941 caacgtcatc caggtcgccc tggccgccta cgccctgcac ctcgacggcg agcgcacgac
     3001 cgacgcctgg gtggtcccgc tccagcaggt cgtctaccgc cagctcatgt acctggtggt
     3061 catccagtcg gcgctcagcg ccctggccgg gacccgcctg cggtggcaca agctggcccg
     3121 caccggcgag gtgatcgtgc ccccgctcgc cccatgacgc ccacgcgccc cccggctacc
     3181 gcgtccgggc cccaaagtcc gacaattccg actagcgacg cccgcccgag cggcctactc
     3241 tcgggtccgc tgcctcgagc ccacctgggc ggctccgagc agggggcgtc ggaggaggcg
     3301 gtgacctcga acgagaacga tgagaccaac ggagccttct gggtctccac gagcgagatg
     3361 gccggcgtct cgaccatcgt gatccagggc gagctcgacc tggccacggc cccgctcgtc
     3421 acggcgaagc tggaggaggc cttctccatt ccgggctcgc tcgtcatcga cgtgacctac
     3481 atgggcttca tggactcctc ggggctgggc atcctggcca aggccttccg ccgggcccgg
     3541 accgacggcc ggagcctggc cttgcgcaac ccctcgcctc ccatacgcag gacgctcgag
     3601 atcgccggat tggacttcct catcgaacgc cgcgactgag cccgccggtg cgcaagccct
     3661 cagttcggtc ggcgccctgc cgatgtcatg agggccgatc cgatgacggc cggtccgatg
     3721 agggcgagca cgcaggatga ccattggcgc agagttcgac accctgctcg ccgcggccca
     3781 ggccggcggg gagtgggcct tcgcggccct ctaccgtgac ctgaacccca agctgctgcg
     3841 ctacctgagc gcccacgcgc cgggcgcggc cgaggacgtc gctgccgaga cctggctggg
     3901 cgtggccagg gggctgcgct cgttcacggg cggggagggc gccttccgca gctggttctt
     3961 caccatcgcc caccgccagc tcgtgcagct ctggcgcgac cgcggccggc gcccctcgag
     4021 cccggccgat cccgagtcct tcgccgacct cgccgccccg gacgaccccg aggccgccgg
     4081 cgtggccatc gtctcggccg ccgaggccgc ccgcaccatc cgggccgcgc tgtcgcggga
     4141 ccaggccgac gtcgtgctgc tgcgggtgct cgccggcctc gacgccgacc aggtggccga
     4201 ggtgctcggc aagcgaccgg caaccgtccg ggtgcttcag caccgggccc tgcggaagct
     4261 ggagggccag ttcagcccgg agccggtaac gagaaggccc cccggcgcga ttgcaccggt
     4321 gacatgaccc gtcaccacct ggacgacatc gaggccggac ggctgctggc cgggaggctc
     4381 gctcccgagg acgccccgcc gggctacgcc ggcctggcca ccctggtgcg cgcggcggcg
     4441 gggcccgcga cggtggcgga gctggccggg gaggccaccg tcgtggccgc cgccctcacg
     4501 gtgctcggcg cggcaggcgc cggcgatgct cgcagccggc gctcctccag gtgggtcagg
     4561 gccttgacgg cgaaaattgc ggccgtcgcg gtcgtcacgg tggccggcgc caccgcagcc
     4621 gccgcggcga cgggcagcct gcccgccccg gcgcagacgg cggtgtccga tgccgcctcc
     4681 cacctgggca tctctgtgcc cgaggccgac caccacggcc cggccgcctc caccggcgcc
     4741 ccgaccgggc accgcggcgc gcccaccccc aaggtggccg gctcgggcca ggacgccaac
     4801 accttcgggc agtgcaccgc gttcctggcc ggcaccgcgg ccggcaagga cgcctcgacg
     4861 gccttcaagg acttcatcgc cgagcacgac gggaccgtcg cctcgacgac gagcttctgc
     4921 aacacgttcc tggccacccg gaccgccggc cacggccagg cgggtccggg cacccagccc
     4981 tccacgaccg acgatgccgg cggacggtcc acgacctccg accaccccgg caggccggct
     5041 gacaagccgg ccaccacctc caccacgcac ccgggcgacg gcggcggcca tggcgcggcc
     5101 ccggccccga gctccgccgc gcctcgcacc ggcagcagcc acccgtagcc accggcattc
     5161 gagcgcgcca cgccgcccga ggccgtcacg gtcgcccttt ccccggatca ggagcgggcc
     5221 acgaccaggg actggagcag gccggcgatc tggtcgacgc ccgccacgtg gtccacggcg
     5281 tcggcccgcc cgatggtcgc gtggggcatg gagaactgct cggacgaatg ctcgtcggcc
     5341 gcgatgaccg tcccgccgag gtcgtggacc gcggtcgctc ccgtggcccc gtcgtggccg
     5401 cctccggaga gcacgacggc gatggcggcc gcgcccacgc tgatggcgag cgtggtcagg
     5461 agcaggtcgg ccgacggccg gctcggcggc gccgcgcccg agacgatgag ccgcagcgtc
     5521 cggtccggca gcaccagggt gtgcagcccc ggcggggcga ccaccacgac gccgggggtc
     5581 agctcggtgc cctgctcggc gaagcgcacc gggagctggg cccggtgggc caggatggcg
     5641 gccaggccgc tcgcccggat cgggtcgagg tgctggaggg cgatgacggc cgcggggtac
     5701 ccgggcggca gcggcgagag cacccgggcc agcgcgtcga ggccacctgc cgacgacacc
     5761 agggcgacca ccggtccgcc cgctcccgtg cccctcaccc cgacaagcat gccaaagccc
     5821 cgtcccgctg tcgcctaccc cgcagccccg tttcgccggg cgtcctcgag ctgggcgcgc
     5881 acccaccggg ccaggccgtt gcggcgcacg acggccgcgc acgcctcgcg ccggtcccgg
     5941 cgctcccggg cgtccatggt gagggcgtcc cacatggcgg cggcctgctg ctcgatgtcg
     6001 aagggatgga cccgcagcgc gaagtgcccc agctcgtggt cggctccggc gttctccgac
     6061 aggagcagca cgccgtcgcg ctcgttcacc accaccgcct ccttggcgac caggttcatc
     6121 ccgt
//
LOCUS       DASWGG010000136         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1753365, whole genome shotgun sequence.
ACCESSION   DASWGG010000136 DASWGG010000000
VERSION     DASWGG010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753365"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VF954_02390"
     CDS             <1..1170
                     /locus_tag="VF954_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9529959.1"
                     /translation="GSQVPMPRARVAASTAPGAAGSDPSIGAAPGNIPAPGADPSLEP
                     GSNPAAGGPQLPAPSTGGAGGGSASAGAGGGSASDPAAQATAAKVDPGVVDIETVLGY
                     QGGRAAGTGMVLNASGEVLTNNHVVDRATQIVAVSVTTGKRYGATVVGTDPTEDVAVI
                     QLRGATGLPTVPLGDSTALAVGSPVVAIGNAGGAGGTPTASSGRVKALNQTMTASDFG
                     GADAETLHGLIETDAPLQPGMSGGPLATSAARVIGMDTAASVGRRFSSGVSVGFAIPI
                     NHALDIARQIEAGQASATVHIGPVAFLGVSSAAATNGNGVVISGVVPGGPAQSAGLRS
                     GDVVTSVDGQPVSTSQALAAAIRRHQPGETTQLTWTDQSGATRTATVRLGTAPAD"
     gene            complement(1497..1790)
                     /locus_tag="VF954_02395"
     CDS             complement(1497..1790)
                     /locus_tag="VF954_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529960.1"
                     /translation="MEAGDVRSYCEAHGKAVVDGDQAHIMADVAPEALAGLAPVGAAL
                     PQPVRSAQVDQIDLAGDKAVVRITYTGESGAVTVESQWEERDGRPKIVAASVV"
BASE COUNT          314 a          791 c          807 g          288 t
ORIGIN      
        1 ggcagccagg tcccgatgcc ccgggcgcgg gtcgcggcat ccacggcgcc gggtgcggca
       61 ggctcggacc cctccatcgg ggcggcgcca gggaacatcc ctgcgccggg cgccgacccg
      121 tcgctggagc cgggcagcaa cccggcggcg ggcgggccgc agctacccgc cccgtccacg
      181 ggaggcgcgg gcggcggatc agccagtgcg ggcgcgggcg gcggctcggc gtccgatccg
      241 gctgcccagg ccaccgcggc caaggtggat cccggcgtgg tcgacatcga gaccgtgctg
      301 ggttaccagg gaggtcgggc cgccggcacg gggatggtgc tgaacgccag cggcgaggtc
      361 ctgaccaaca accacgtggt ggacagggcg acccagatcg tggccgtgtc ggtcaccacc
      421 gggaagcgct atggcgcgac ggtggtcggc accgacccga ccgaggacgt ggccgtgatc
      481 cagctccggg gggcgacggg gctgccaacc gtcccgctcg gcgactcgac cgcgctcgcg
      541 gtcgggagcc cggtcgtcgc catcggcaac gccggcgggg ctggcggcac cccgaccgcc
      601 agctccggca gggtgaaggc gctcaaccag acgatgacgg cgagcgactt cggcggggcc
      661 gacgcagaga ccctccacgg tctcatcgag accgacgccc ccctccagcc gggcatgtcg
      721 ggcgggccac tggccacgtc cgccgcccgg gtcatcggga tggacacggc ggcctcggtc
      781 ggaaggcgct tcagctcggg ggtgtccgtc gggttcgcca tcccgatcaa ccacgccctc
      841 gacatcgccc gccagatcga ggccgggcag gccagcgcga ccgtgcacat cgggccggtc
      901 gccttcctcg gcgtgtcctc ggcggccgcg accaacggca acggcgtggt catcagcggg
      961 gtcgtgcccg gtgggccggc gcagtccgcc gggctgcgat caggggacgt ggtcacgtcg
     1021 gtcgacgggc agccggtctc gaccagccag gcgctcgccg ccgccatccg gcggcaccaa
     1081 cccggcgaga cgacccagct gacctggacc gatcagagtg gggcgacccg cacggcaacc
     1141 gtgaggctgg ggacggcgcc ggcggactga cggtccggcg gagccatgcg ggccgggtac
     1201 tgagctgggc tgagccgggt cagtcggccg agcaggagga cgctcgctgg tgggcggcca
     1261 ccatgacctc gggctcgagc gttcgagccg acatcagata gcgaccgcag cgcgggcagg
     1321 ggaccgagac cctccactgc gtcgatgagc ccggacggcg ggggcggcga tcaaatctca
     1381 tggctctacc ttccccgatc aaggtcacgt gaagaagtac ggcgtgtaac cagcgcgtga
     1441 cattgcgcgg cgtgacattg cgcggctggg tcgggctgcg ggtggcgaat cggccgtcag
     1501 accaccgagg cggccacgat cttcggccgg ccgtcccgct cctcccattg cgactcgacc
     1561 gtcaccgccc cgctctcgcc cgtgtaggtg atccgcacca ccgccttgtc gccggccaga
     1621 tcgatctggt cgacctgggc ggacctcacc ggctggggca gggcggcacc aaccggcgcg
     1681 aggccggcaa gcgcctcggg ggcgacgtcg gccatgatgt gcgcctgatc gccgtctacg
     1741 acggcctttc cgtgggcctc gcagtagctg cggacgtcgc ctgcttccat ggtgtggctc
     1801 ccctctcgtc ggatgccgcc cgaccaggct gttgtcgctg catcgagtgt cgctggatcg
     1861 agtgttgctg gatcaggtgc cgctggacag ggccggcatg atgaccctat ggccccaaca
     1921 ctatggcatc caccatggca tcgagcaggc ggacgcccgg gcagaccctc agcaccaggt
     1981 cagacggggt taactggcgg ccagacgcca tggtccggct cctaggatcc cgggcatgac
     2041 ctcgtcgcaa ccctcgaagg cggcggggcc ggctgcggag gagccgtccc cggaggggta
     2101 ccgtcagccc cgcttcgccc gcccttccgg ggccaccgag ctgcttctcg tccggcacgg
     2161 agagtctgct cccgccaacc tcgacgagcc cttcccgctc
//
LOCUS       DASWGG010000137         4152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1110743, whole genome shotgun sequence.
ACCESSION   DASWGG010000137 DASWGG010000000
VERSION     DASWGG010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110743"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VF954_02400"
     CDS             <1..962
                     /locus_tag="VF954_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529961.1"
                     /translation="AGLEGADFADGVPKGSSPLRLVTGSAGGFVTARGGLLGKERALE
                     LHVAVNCCHGGPGEDGTLQGVLDSAGVRYTGPTVAGAAIGMDKLAFGALVDAAGLPSL
                     PRVALTAGTDKLEFEGPYIVKPRFGGSSIGIDVVEDLETARQRLSRNVHLAAGAVVEP
                     YRPGSVDLEIALRAFPSLQLSAIDKPERVTAGGEILSYRDKYVGGEGMSSAPRQLPAR
                     IDPAVGARVREAATVVADLVDVRGIARLDFLLEGDDLYVNEINTIPGSLSWHMWVDPA
                     IPFATLLSDMLEEAARRPSIRYSTAGADGTALRSAGTIQGKLG"
     gene            962..1510
                     /locus_tag="VF954_02405"
     CDS             962..1510
                     /locus_tag="VF954_02405"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX9529962.1"
                     /translation="MTHVAVTAVGVDRPGIVAAVTGVLKDEGCNLEDTSMTILRGHFA
                     MMLVVEIPPDASPSLLEAALAPVGEQLRLVLTVRPVDEGTEERTGAAPAGEQWAVAVY
                     GADRPGIVHAVATVLSGVGANITDLTTRVIGESGHPVYAMHLDVVVPAGVDAGALADH
                     LSLTAAELGVDVSIHPAEADIL"
     gene            1529..2029
                     /gene="def"
                     /locus_tag="VF954_02410"
     CDS             1529..2029
                     /gene="def"
                     /locus_tag="VF954_02410"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX9529963.1"
                     /translation="MAVRPVLRLPDPVLKRPAGPVGRVGSAARALGQDLLDTMRASPA
                     CVGLAAPQIGVGLRAFCVDVTGHPKAVSCHGELVMFDPEVLVARDPVLAREGCMSVPD
                     LTGDVARASGVVVRGTGLDGAERVVSVDAFEARAVLHEMDHLDGLLFLDRVGSAAALH
                     ARRVYR"
     gene            2063..2677
                     /locus_tag="VF954_02415"
     CDS             2063..2677
                     /locus_tag="VF954_02415"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX9529964.1"
                     /translation="MAFPRKLLNEGEDVVLDLRPHWSTLTGPVAAVVIVLAGAVAALV
                     LGAPAWTAYPILGLVALAALWLAVRWLRWTTTNFTVTNDRIVYRSGVLAKKGREIPLD
                     RVNDISFNQGVFERIIGAGDLMIESAGQHGQERYTDIPRPSKVQNVIYREMERFRGRE
                     ADRASGHHELSVPEQLEKLDELRQRGVISQEEFDQKKAQLLERM"
     gene            2743..3390
                     /locus_tag="VF954_02420"
     CDS             2743..3390
                     /locus_tag="VF954_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX9529965.1"
                     /translation="MVTTLPQTLANLAPTRRSILLLLKKAGSARAEELAGSLGLTVSA
                     LRQHLSALAADGLVGHEQVRSGPGRPKHLFHLTPAAEGYFPKFHAELTNELLAYVEAE
                     DPELLDRLFTRRRQRRIEGARERLAGRDLRARVEELTAILDGDGYLADWVEVGEGRYR
                     IVEHNCAILGVAMRYGQACSSELDFIRAVLPDAEVERVSHIVHGARMCAYEVRAA"
     gene            3488..>4152
                     /locus_tag="VF954_02425"
     CDS             3488..>4152
                     /locus_tag="VF954_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9529966.1"
                     /translation="MTGPLGGVRIVELAGIGPGPFAGMMLADMGADVIRVDRAAAARP
                     AVDRPPTELLNRSRRSIGVDLKHPDGVETVLKLVEQADAMFEGFRPGVTERLGLGPED
                     CMARNPRLVYGRMTGWGQDGPYAHAAGHDINYIALAGTLGAIGRRGEAPVPPLNLVGD
                     FGGGGMLLAFGLVCAILEAKSSGRGQVVDAAMVDGAAVLATMTHGMRAMGAWKDEWGT
                     NLL"
BASE COUNT          574 a         1455 c         1533 g          590 t
ORIGIN      
        1 acgccggcct cgaaggcgcg gacttcgccg acggcgtgcc gaaggggtcg tcgccgctgc
       61 ggctggtgac gggctcggcc ggggggttcg tgacggccag gggcggcctg ctcggcaagg
      121 agcgggccct cgagctgcat gttgccgtca actgctgcca cgggggcccg ggtgaggacg
      181 gcacgctcca gggtgtgctc gactccgcgg gcgtgcggta cacggggccc accgtcgccg
      241 gcgccgccat cggcatggac aagctggcct tcggcgccct cgtggacgcg gccggcctgc
      301 cctcgctgcc ccgggtcgcg ctgaccgcgg gcacggacaa gctggagttc gaaggcccgt
      361 acatcgtcaa gccccggttc gggggatcgt ccatcggcat cgacgtggtc gaggacctgg
      421 agacggcgag gcagcggctg tcccgcaacg tccacctcgc cgccggcgcg gtcgtggagc
      481 cctaccggcc cggctccgtc gacctggaga tcgccctgcg ggcctttccc tcgttgcagc
      541 tgtcggccat cgacaagccg gagcgggtca ccgcgggggg cgagatcctc agctatcgcg
      601 acaagtacgt cgggggcgag ggcatgtcgt cggcgccgcg ccagctgccg gcccggatcg
      661 atcccgccgt gggcgcccgc gtccgcgagg cggccaccgt cgtcgccgat ctggtcgacg
      721 tcaggggcat cgcccgcctg gacttcctgc tcgagggcga cgatctctac gtcaacgaga
      781 tcaacaccat cccgggctcg ctcagctggc acatgtgggt ggacccggcc atccccttcg
      841 ccaccctgct gtccgacatg ctggaggaag ccgcccggcg accgtcgatc cgctactcga
      901 cggccggggc cgacgggacg gcattgcggt cggcgggcac catccagggc aagctgggct
      961 gatgacccac gtggcagtga cggcggtcgg ggtcgaccgc ccgggcatcg tggcggcggt
     1021 gaccggtgtc ctcaaggacg agggttgcaa cctggaggac acgtcgatga cgatcctccg
     1081 cggccatttc gccatgatgc tcgtcgtcga gatcccgccg gacgcgtcgc cgtccctgct
     1141 cgaagccgcc ctcgcccccg tcggcgagca gctccgcctc gtgctgaccg tgcgccccgt
     1201 cgacgagggg acggaggaac ggaccggcgc ggcaccggcg ggtgagcagt gggccgtcgc
     1261 cgtctacggg gcggaccggc cgggcatagt ccacgccgtc gccacggtgc tctcgggtgt
     1321 gggcgccaac atcaccgatc tgacgacccg cgtcataggc gagtccggcc accccgtcta
     1381 cgccatgcac ctcgacgtcg tcgtcccggc cggggtggac gcgggcgcgc tcgcggacca
     1441 cctctcgctg acggcggcgg agctgggcgt tgacgtgtcc atccacccgg ccgaggccga
     1501 catcctctag tgatcgccta ggacggccat ggcggtcaga ccggtgctcc ggctccctga
     1561 tcccgtcctc aagcgcccgg ccggtcccgt ggggcgggtg ggctcggcgg cacgcgccct
     1621 cggccaggac ctcctcgaca ccatgcgggc gtcccccgca tgcgtcgggc tggccgcgcc
     1681 acagatcggc gtcggactcc gcgccttctg cgtggacgtg acggggcacc cgaaggccgt
     1741 ctcatgccac ggagagctgg tgatgttcga cccggaggtg ctcgtggccc gcgacccggt
     1801 gctcgcccgc gaggggtgca tgagcgtgcc cgacctcacg ggtgacgtcg cccgggcgag
     1861 cggggtagtc gtccggggca cgggtctcga cggagccgag cgcgtcgtgt cggtcgacgc
     1921 cttcgaggcc cgggccgtgc tgcacgagat ggaccacctc gacggccttc tcttcctcga
     1981 ccgggtgggc tcggccgcgg cgctgcacgc ccgccgggtc taccggtagg aagtggctgg
     2041 gtggctggct accctttccc ccgtggcgtt tccccgcaag ctgctcaacg aaggggagga
     2101 cgtggtcctc gacctgcgtc ctcactggtc gaccctcacc gggcccgtcg ccgccgtcgt
     2161 gatcgtgctc gccggggccg tcgccgcgct ggtgctcggg gccccggcct ggacggcgta
     2221 cccgatcctg ggcctggtcg ccctggccgc cctgtggctc gcggtgcgct ggctgcggtg
     2281 gacgacgacc aacttcaccg ttaccaacga ccggatcgtg taccgcagcg gcgtgctggc
     2341 caagaaggga cgggagatcc cgctcgaccg cgtcaacgac atctccttca accagggcgt
     2401 gttcgagcgc atcatcggcg ccggcgacct gatgatcgag tctgccggcc agcacggcca
     2461 ggagcgctac acggacatcc cgcgtccctc caaggtgcag aacgtcatct accgcgagat
     2521 ggagcgcttc cgcgggcggg aggccgatcg cgcctccggg caccacgagc tgtccgtgcc
     2581 cgagcagctg gagaagctcg acgagctgcg ccagcggggc gtcatctcac aggaggagtt
     2641 cgaccagaag aaggcccagc tgctcgagcg gatgtgagcg cggcccggag gccaagaggc
     2701 gacctctggt ttgacaagga gagtctgtgt aaaattgacc cgatggtgac caccctgccc
     2761 cagacgctcg ccaacctggc gcccactcgg cggtcgatcc tcctgctgct gaagaaggcc
     2821 ggctcggccc gggccgagga gctggccggc tcgctcggcc tcacggtgag cgccctgcgg
     2881 cagcacctgt ccgccctggc ggccgacggc ctggtaggcc acgagcaggt gcggtccggg
     2941 ccaggccggc ccaagcacct gttccacctg acgccggcgg ccgagggcta cttcccgaag
     3001 ttccacgccg agctgaccaa cgagctgctg gcctatgtcg aggccgagga ccccgagctg
     3061 ctcgatcggc tcttcacccg gcggcgccag cgccgcatcg agggggcgcg ggagcggctg
     3121 gccggccgcg acctgcgggc gcgggtcgag gagctgaccg ccatcctgga cggggacggg
     3181 tatctcgccg actgggtcga ggtgggcgag gggcgctacc gcatcgtcga gcacaactgc
     3241 gccatcctcg gggtggccat gcgctacggg caggcgtgca gcagcgagct ggacttcatt
     3301 cgggccgtgc tgcccgacgc cgaggtcgag cgggtgtcgc atatcgtgca cggggccagg
     3361 atgtgcgcgt acgaggtgcg ggccgcttga ccaggcggtc aagtctgcag caaagtcgga
     3421 ccatggcgaa cagagggccg aacagagggt cgagcacagg gccgaacgga cacagggtgg
     3481 ggcagacatg accgggccgc tgggcggtgt gcgcatcgtg gagctggccg gcatcggccc
     3541 cggccccttc gccgggatga tgctcgccga catgggcgcc gacgtgatcc gcgtggaccg
     3601 ggccgcggcg gcgcgccccg ccgtggaccg cccgcccacg gagctgctca acaggagccg
     3661 ccgctccatc ggcgtcgacc tcaagcaccc ggacggcgtg gagaccgtcc tgaagctggt
     3721 cgagcaggcc gatgcgatgt tcgagggctt ccggccgggc gtgaccgagc gcctcgggct
     3781 cgggccggag gactgcatgg cgcgcaaccc caggctggtc tacggccgca tgaccgggtg
     3841 gggccaggac ggcccctacg cccacgcggc cggacacgac atcaactaca tagcgttggc
     3901 gggcacgctc ggtgccatcg gccgcagggg cgaggcgccc gtcccgccgc tcaacctggt
     3961 gggcgacttc ggcggcggcg ggatgctgct cgccttcggc ctggtgtgcg ccatcctcga
     4021 ggccaagtcg tccgggcggg gccaggtcgt ggacgcggcc atggtcgacg gggcggctgt
     4081 gctcgcgacg atgacgcacg ggatgcgggc catgggtgcc tggaaggatg aatggggcac
     4141 caacctgctg ga
//
LOCUS       DASWGG010000138         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1110980, whole genome shotgun sequence.
ACCESSION   DASWGG010000138 DASWGG010000000
VERSION     DASWGG010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110980"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VF954_02430"
     CDS             <1..623
                     /locus_tag="VF954_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9529967.1"
                     /translation="LVGEIVSRAASDRVPVRTVARARFDDAARTQAPQGVLAHAEPVA
                     VTPLDELAAGRAPFLVVFDGLTDPQNLGALLRTAEVAGATGAVLPRHRSAHLSPAAVK
                     AAAGAVEHLRFALVPGVPAALLRLGELGVWRVGLDAGGDRSVFELELGREPLALVVGA
                     EGAGLSRLGRARCDVVAAVPQAGALPSLNVAAAGAVACFHILRGRQ"
     gene            823..1476
                     /gene="sigH"
                     /locus_tag="VF954_02435"
     CDS             823..1476
                     /gene="sigH"
                     /locus_tag="VF954_02435"
                     /inference="COORDINATES: protein motif:HMM:NF006145.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HEX9529968.1"
                     /translation="MALRIETIPLQELADDELVARFHQGDAGAVETLLDRYRRFARAK
                     ARGYFLVGADSDDIEQEGMIGLYKAARDFRPDRQASFRAFAELCITRQVITAVKTATR
                     QKHQPLNHYVSISGVRGGDDPAERTIEDLLADHHEADPADTVVSRERMDQMRRSMAEM
                     LSGLEVDVVRLYVEGKSYQEIGEQLGRQTKSIDNALQRIKRKLDVHLQEDAGRAFSG"
     gene            1624..2838
                     /locus_tag="VF954_02440"
     CDS             1624..2838
                     /locus_tag="VF954_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529969.1"
                     /translation="MSTGGIGRALAAPTFTPVVIQPSQGLNLGEPGVAVASDGSAIYV
                     DAPPGIDIPSDVFKSTNGGTSWVTTPLSMRANLPGGGDSNLAADPGSASTLYMTDLWL
                     GSATVSVTHDGASTWSANPVQGVVVQDRQWIATPGGGIAYHLTHQIPAGLVVSRSVDG
                     GTTYPQHVVAATPADQTGCICPPGNLISEGGSLLGGGKAGLVYSTSTGGVNFAYSTNS
                     GLSFTNVPVRASDSASDTTRAFPVVANAGNNHLVAVWLETDANGSAWSQVGFSESTNW
                     GASWSTPTYIVDKTGGSVYPWVAANGTKVAVSLYHTATAGAPDSMAAGAQWFETYLER
                     VSGVWTAPVTVDPTAVKSGPVCTQGTGCSANRELLDFQTAALDPTGHADLTWTHSIDN
                     VSKTELRFAHEA"
     gene            complement(2924..2997)
                     /locus_tag="VF954_02445"
     tRNA            complement(2924..2997)
                     /locus_tag="VF954_02445"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2961..2963),aa:Thr,seq:tgt)
BASE COUNT          514 a         1195 c         1212 g          503 t
ORIGIN      
        1 gcctggtggg cgagatcgtc tcgcgggccg catccgaccg ggtgccggtc cgcaccgtgg
       61 cccgcgcccg attcgacgac gccgcccgga cccaggcccc ccagggcgtg ctcgcccacg
      121 ccgagcccgt cgcggtgacg ccgctcgacg agctcgctgc cggccgggcg ccgttcctgg
      181 tcgtgttcga cggcctgacg gacccccaga acctcggggc cctcctgcgc accgccgagg
      241 tggccggcgc gacaggggcg gtgctgccca ggcaccggtc ggcgcacctg tcgccggcgg
      301 cggtcaaggc ggccgcgggg gccgtggagc acctgcggtt cgcgctcgtg cccggcgtcc
      361 cggccgcgct gctgcgcctg ggagagctgg gtgtctggcg ggtgggcctg gacgcggggg
      421 gcgatcgctc ggtgttcgag ctggagctgg gccgcgagcc gctcgccctc gtggtgggcg
      481 ccgagggcgc cggcttgtcc cggctcgggc gcgcccgctg cgacgtggtg gccgccgtcc
      541 cgcaagccgg cgccctgccc tcgctgaacg tcgccgccgc cggggcggtg gcctgcttcc
      601 acatcctgcg gggccggcag tgactagtcc gttcggggcg gttccgggca aatggggctt
      661 ccatgggcaa gaaaagttgt ccggaaagga gggtttggcc gatttagccg tggtgtgacg
      721 gttgacactc agggaatgga ctggttacaa ctaactagtc cttccagatg tagtcgcctg
      781 cacacgacat caccccctcg tcacaccacg gtaggagcag acatggccct gaggattgaa
      841 acgatccccc tccaggaact ggccgacgac gagctcgtgg cccgcttcca ccagggcgat
      901 gccggcgccg tggagaccct gctggatcgc taccggcgtt tcgccagggc caaggcccgg
      961 ggctacttcc tggtcggcgc cgactccgac gacatcgagc aggaggggat gatcggcctg
     1021 tacaaggcgg cccgcgactt ccgccccgac cgccaggcgt ccttccgggc cttcgccgag
     1081 ctgtgcatca cccgccaggt catcaccgcg gtcaagaccg ccacccggca gaagcaccag
     1141 cccctcaacc actacgtctc catctcgggg gtgcggggcg gcgacgaccc ggccgagcgg
     1201 acgatcgagg acctcctcgc agatcaccac gaggccgacc cggccgacac cgtggtctcc
     1261 cgcgagcgca tggaccagat gcgccgttcc atggccgaga tgctgtcggg gctcgaggtc
     1321 gacgtggtgc ggctctacgt cgaaggcaag tcctaccagg agatcggcga gcaactcggg
     1381 cgccagacca agtcgatcga caatgccctc cagcgcatca agcgcaagct ggacgtccac
     1441 ctccaggagg acgccggccg ggccttctcg ggctgaccgc gcaagattta ccggattgcc
     1501 acgttccctc cggggcgttc gtggggaagg gatcgcatat tcccgcgcgc taggagaaca
     1561 aggatgcaga cacgtcgtcg acgtatcgct cccgccatcg gagcggtcgc cgtgctggtg
     1621 gggatgtcca ctgggggtat cggccgcgcc ctggcggcgc cgacgttcac gccggtggtg
     1681 atccagccct cgcaaggcct caacctcggc gagcccggcg tggcggttgc gtcggacggc
     1741 tcggccatct acgtcgacgc gcccccgggc atcgacatcc cgagcgacgt gttcaaatcc
     1801 accaacggcg gcacgtcatg ggtcacgacg cccttgtcca tgcgtgccaa cctgcccggg
     1861 ggcggcgact ccaacctggc cgccgacccc ggtagcgcta gcaccctgta catgaccgac
     1921 ctgtggctgg gaagcgcgac ggtgtcggtg acccacgacg gcgccagcac gtggtcggcg
     1981 aaccccgtgc agggcgtcgt cgtgcaggac cgacagtgga tcgccacccc cggtggcggg
     2041 atcgcctacc acctgacgca tcagatcccg gccggcctgg tggtgtcgcg gtcggtggac
     2101 gggggcacga cctatcccca gcacgtcgtg gccgccaccc cggccgacca gaccggctgc
     2161 atctgcccgc ccgggaacct catctccgag ggcgggtccc tgctcggcgg cgggaaggcg
     2221 gggctcgtct actcgacctc gaccggcggc gtgaacttcg cgtactccac caacagcggc
     2281 ctgagcttca cgaatgtgcc ggtacgggcc agcgactccg cgtccgacac cacccgggcc
     2341 ttccccgtgg tcgccaacgc cgggaacaac cacctcgtgg cggtgtggct ggagaccgac
     2401 gccaacggca gcgcctggag ccaggtcggc ttcagcgagt cgaccaactg gggcgcgtca
     2461 tggagcaccc cgacctacat agtcgacaag acgggcgggt cggtctaccc gtgggtggcg
     2521 gcgaacggca ccaaggtggc cgtctcgctc taccacacgg ccacggccgg ggcgccggac
     2581 agcatggcgg ccggcgcgca gtggttcgag acctacctcg agcgggtgag cggggtgtgg
     2641 accgcgccgg taacggtcga tcccaccgcg gtcaagtccg ggcccgtctg cacccagggc
     2701 accgggtgca gcgccaaccg tgagctgctc gacttccaga ccgccgcgct ggacccgacc
     2761 ggtcacgccg atctgacctg gacccactcg atcgacaacg tgtccaagac cgagctgcgg
     2821 ttcgcccacg aggcgtaggg aggcgtaggg atcggggtac gccgggtcgg ggggcccggc
     2881 gtacccctcg cccgggcggg gacgggacgg gagggaggga gggagcccga ggtgggactc
     2941 gaacccacga ccacccgctt acaaggcggg tgctctggcc aactgagcta ctcgggcgcg
     3001 ggcgaatcgt agctggtcag aggccccttt cccgacagtg ttcgcctgtg gataagtggg
     3061 gcagtcacgg ggacggaacc agcggaaggg cggatcgaat tgcactcggt cgggtgttgg
     3121 ccacgcaacc cgcctgatcg agcttctcca ttggcgatgc cggaatcttg gccaccagcg
     3181 cccctttctg ctggacggac ccgtggtaga acgagcccgt gcacggcgcg gacagtgatc
     3241 gcgggaggtc gccggtccag gtggtcctgg aggttatggc cgccgccgcc gagggccggg
     3301 tcgaggaggt gctcgcgctc acagaccccg aggtcgtctg gcgtccgata ttccgtcctg
     3361 gccgggccct gtacaggggt cacgcggaat tgggcctctt tctttctgac cggcgttccg
     3421 cgtg
//
LOCUS       DASWGG010000139         5720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_389630, whole genome shotgun sequence.
ACCESSION   DASWGG010000139 DASWGG010000000
VERSION     DASWGG010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389630"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..939)
                     /locus_tag="VF954_02450"
     CDS             complement(25..939)
                     /locus_tag="VF954_02450"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HEX9529970.1"
                     /translation="MLSNESEELLTWMGVERGRSANTLAAYRRDLAAYEEFLRGRGVA
                     LADVPERLVEDYVAFLRAAGRRPASIARALVAVRSLHRFCLDEGQATTDPSSEVGLPR
                     VPQGLPKALTEEEIDGLLAAAAGEGATGRRDRAILEVLYGTGVRISELVGLSLADLVA
                     LDEGSVRVFGKGAKERVVPVGRMARQALADWLTLSGRGALAPERWARRGDAEAVFLNA
                     RGGRLSRQGAWAIVRRYGHRAGLGDRLTPHVLRHSCATHMLDHGADIRVVQELLGHAS
                     VSTTQVYTKVSPERLRAVYEAAHPRAKR"
     gene            complement(950..1492)
                     /locus_tag="VF954_02455"
     CDS             complement(950..1492)
                     /locus_tag="VF954_02455"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9529971.1"
                     /translation="MTRFSKLAEEGLYRGPVISLARGRFAGPEGELFERDVVHHPGAV
                     SVVPVVEDGAAALLVVQYRAAVDAELLEIPAGKRDVDGEEPVATARRELEEEVGRRAG
                     RMELLARFYNSPGFCDEDSWVFLALDLEACEASAQGLEERHMVVRTVTLDDVPGMIAR
                     QEIRDAKTIIGLTLARERLA"
     gene            complement(1467..3146)
                     /locus_tag="VF954_02460"
     CDS             complement(1467..3146)
                     /locus_tag="VF954_02460"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX9529972.1"
                     /translation="MIVSLRRDPLTKHIFVTGGVASSLGKGLTASSLGRLLKSRGMRV
                     TMQKLDPYINVDPGTMNPFEHGEVFVTEDGGETDLDLGHYERFIDENLHRESNATTGS
                     IYQSVIAQERRGDFLGKTVQVIPHVTNEIKDRIKRLATDGVDVVITEIGGTVGDIEIL
                     PFLEAIRQFRKDVGRDNVCYIHLTLVPYIGPSGEQKTKPTQHSVTELRSRGIQPDIIV
                     CRSDKPISEALKRKISMLCDVPVEAVISNVDARNVYEIPLMLHLDGLDDYVCRILRFD
                     DDEHVLDLSAWEALVERVEAATTPVRIGLIGKYVNLPDAYLSVVEALRHAGFAHGAAV
                     EIDWIQAEEVTGLLAEGRLRHLDGMVIPGGFGERGIEGKIAAAGYAREQAVPCLGLCL
                     GLQVMVIEYARSVCGMPDANSREFDTRSNHLVIDLMDEQKELVEMGGTMRLGAYPARL
                     LAGSRVAQAYGEPVVYERHRHRYEVNPRHRRRLEDGGLLCSGTSPDDRLVEFVELPGH
                     PCWMGTQAHPEFKSRPDRPHPMFLELVEAAVARAEGRSPRLLELDAVQQAR"
     gene            complement(3165..4775)
                     /gene="recN"
                     /locus_tag="VF954_02465"
     CDS             complement(3165..4775)
                     /gene="recN"
                     /locus_tag="VF954_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX9529973.1"
                     /translation="MLTEISVHDLGVLADLRLALGPGMTAITGETGAGKTLVVEAIEL
                     LVGGRADSVLVRPGAAEAVVEGRFLVHDDEHVLARVVPSGGGRSRGYLDGRMCTAGAL
                     AELGAGLVDLYGQHAHQSLLGAASQRDALDAFAAVDLGPRTAARSRRREIDEQLASLG
                     GDARARARETDLLRFQLDEIDGAGLADPDEDVRLEAEEDALSAATAHREAAEAARHAV
                     SGDGGVLDGLGQAVGALAGRAPLAGPETRLRDLVAAAQEVGAELRHAAERLQDDPDRL
                     AEVRSRRHRLRELGRKYGATVADIVAYAAETRRRLADLESHGAKVARLEAERQDVDAA
                     LARAEDAVGRQRRTGAGGLAQAVEANLRDLAMPRGRVEVRVGPGAGDEVAFLLGANPG
                     EPVLPLSKVASGGELARAMLALRLVLSAGPPTLVFDEVDAGIGGEAAVAVGRALARLS
                     PQRQVLVVTHLPQVAAFADHHVAVVKGEARGRTVAEAAALDGASRVAELSRMLSGQPA
                     SATARRHAGELLELAASERSHRAAGALR"
     gene            complement(4775..5632)
                     /locus_tag="VF954_02470"
     CDS             complement(4775..5632)
                     /locus_tag="VF954_02470"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX9529974.1"
                     /translation="MGDVVLVIHPDRPEAVRLAVETAGWLGSRGHGVRISERDAATAG
                     LEHAGVADDMLVDGATLAVSLGGDGTMLRAVELAAPHGVPVLGVNLGHLGYLTEVEPA
                     HLALALERFWAGEHTIEERMTLEARWLRAGRDEEETAMLALNEVVVEKGTSGNTVNVA
                     VGIDGRPFLTYAADGLIVATPTGSTAYNLSARGPIVSPHLRAILITPVSPHMLFDRSL
                     VLEPEEEVRLDILDGSRAELLLDGRSTGELVAGDAVTCRAATPAKLVSFGARDFRHIL
                     KAKFGLADR"
BASE COUNT          750 a         2141 c         2068 g          761 t
ORIGIN      
        1 cggcgaagga gcgagcgaga aagctcagcg cttggcccgg gggtgtgcgg cctcgtacac
       61 ggcgcggagc cgttcgggcg agaccttcgt gtacacctgg gtcgtcgaca cgctcgcgtg
      121 accgagcagc tcctgcacga cgcggatgtc ggcgccgtgg tcgagcatgt gggtggcgca
      181 ggagtggcgc agcacgtgag gggtgagccg gtccccgagg ccggcccggt gcccgtatcg
      241 ccggacgatc gcccacgccc cctgccggct gaggcggccg ccccgggcgt tcaggaacac
      301 cgcctcggcg tccccccgcc gggcccaacg ttccggggcg agcgcgcccc gccccgacag
      361 ggtcaaccag tcggccaggg cctggcgggc catcctcccg acgggcacga cccgctcctt
      421 ggcgcctttg ccgaagaccc tgacgctgcc ctcgtccagg gccaccaggt ccgccagcga
      481 cagcccgacc agctcggaga tgcgcacgcc ggtcccgtag agcacctcga ggatggcccg
      541 gtcgcgccgt ccggtggcgc cctcgcctgc cgccgcggcg agcagcccgt cgatctcctc
      601 ctcggtgagg gccttgggca ggccctgggg cacccggggc aggccgacct cgctgctcgg
      661 gtccgtcgtg gcctggccct cgtctaggca gaagcggtgc aggctgcgca ccgcgaccag
      721 ggcccgggcg atggacgccg gccggcgccc ggcggcccgc aggaacgcca cgtagtcctc
      781 caccagccgc tccggcacat ccgccagggc cacgccccgc ccgcgcagga actcctcgta
      841 ggcggccagg tcccggcggt aggccgcgag cgtgttggcc gaccgccccc gctcgacgcc
      901 catccaggtc agcagctcct ccgactcgtt ggagagcacc gtcagggggt cacgccaggc
      961 gctcgcgggc cagggtgagg ccgatgatgg tcttggcgtc gcggatctcc tggcgggcga
     1021 tcatcccggg cacgtcgtcg agggtgacgg tgcgcaccac catgtggcgc tcctcgagac
     1081 cctgggccga cgcctcgcag gcctcgagat ccagggccag gaacacccag gagtcctcgt
     1141 cgcagaaccc cggcgagttg tagaaccggg ccagcagctc cattcggccg gcccgccggc
     1201 cgacctcctc ctccagctcc cggcgggccg tggccaccgg ctcctcgccg tcgacgtcac
     1261 gtttccccgc cgggatctcc agcagctcgg cgtccacggc ggcgcggtac tgcaccacga
     1321 ggagggcggc cgcgccgtcc tccaccacgg gtacgaccga caccgcgccg ggatggtgca
     1381 cgacgtcgcg ctcgaagagc tcgccttcgg gaccggcgaa gcgcccccgg gccaggctga
     1441 tcaccgggcc ccggtacagg ccctcctcag cgagcttgct gaaccgcgtc aagctcgagg
     1501 aggcggggcg accgcccttc tgcccgggcc acggccgcct cgaccagctc gaggaacatc
     1561 gggtgcggcc ggtcgggacg gctcttgaac tccgggtggg cctgggtgcc catccagcac
     1621 gggtgcccgg gcagctccac gaactccacc agccggtcgt ccggcgacgt ccccgagcag
     1681 agcaggcccc cgtcctccag ccgccggcgg tggcgggggt tgacctcgta acggtggcgg
     1741 tggcgctcgt acaccaccgg ctccccgtag gcctgcgcca cccgggaacc ggccagcagc
     1801 cgggccgggt aggcgcccag gcgcatggtg cctcccatct cgacgagctc cttctgctcg
     1861 tccatgaggt cgatgacgag gtggttgctg cgcgtgtcga actcccggga gttggcgtcg
     1921 ggcatgccgc acacgctccg ggcgtactcg atgaccatca cctggaggcc gaggcagagc
     1981 cccaggcagg gaacggcctg ctcccgggcg tagccggcgg ccgcgatctt gccttcgatc
     2041 ccccgctccc cgaacccccc cgggatcacc atgccgtcca ggtggcgcag ccggccctcg
     2101 gccagcagcc ccgtgacctc ctcggcctgg atccagtcga tctccaccgc cgccccatgg
     2161 gcgaacccgg cgtggcgcag ggcctcgacc accgacaggt aggcgtctgg caggttcacg
     2221 tacttgccga tcaggccgat gcgcacgggg gtggtcgcgg cctcgacccg ctccaccagg
     2281 gcctcccacg ccgacaggtc gagcacgtgc tcgtcgtcgt cgaagcgcag gatccggcac
     2341 acgtagtcgt cgagcccgtc gaggtgcagc atcagcggga tctcatagac gttgcgggcg
     2401 tccacgttgg agatcacggc ctccaccggc acgtcgcaca gcatggagat cttgcgcttg
     2461 agggcttcgg agatcggctt gtcgctgcgg cacacgatga tgtcgggctg gatcccccgg
     2521 ctgcgcagct cggtgaccga gtgctgcgtc ggcttggtct tctgctcgcc ggagggcccg
     2581 atgtagggca cgagcgtcag gtggatgtag cagacgttgt cgcggcccac gtccttgcgg
     2641 aactgccgga tggcctcgag aaagggcagg atctcgatgt cgcccaccgt gccgccgatc
     2701 tcggtgatca ccacgtcgac cccgtcggtg gccagccgct tgatgcggtc cttgatctcg
     2761 ttggtgacgt gcgggatgac ctgcaccgtc ttgccgagga agtcgccccg gcgctcctgg
     2821 gcgatcaccg actggtagat cgagccggtg gtggcgttgg actcccggtg caggttctcg
     2881 tcgatgaagc gctcgtagtg gccgaggtcg aggtcggtct cgcccccgtc ctcggtgacg
     2941 aagacctcgc cgtgctcgaa ggggttcatg gtgccggggt cgacgttgat gtaggggtcc
     3001 agcttctgca tcgtgacccg catgccccgg ctcttgagca gccgccccag cgaggaggcc
     3061 gtgaggccct tgccgagcga gctggccacg ccgcctgtca cgaagatgtg cttcgtcaaa
     3121 gggtccctcc ggagcgagac gatcacccga gacaacggac caacctaccg cagggcgccg
     3181 gctgcccggt gggaccgctc ggaggcagcc agctccagca gctcgcccgc gtggcgccgg
     3241 gccgtggcgc tggcgggctg gccggacagc atccgggaca gctccgccac ccgtgacgcg
     3301 ccgtcgagcg cggcggcctc ggccacggtg cggcccctgg cctcaccctt cacgaccgcg
     3361 acgtggtggt cggcgaaggc ggccacctgg gggagatggg tcaccaccag cacctggcgc
     3421 tgcgggctca ggcgggcgag cgcccggccg acggccaccg cggcctcacc gccgatgccg
     3481 gcgtccacct cgtcgaagac cagggtcggc ggccccgcgc tgagcaccag ccgcagggcg
     3541 agcatggccc gggccagctc gccgccgctc gccaccttgc tcaggggcag gactggctca
     3601 ccggggttgg cgcccagcag aaacgccacc tcgtcgcccg cccccggccc gacccggacc
     3661 tccacccggc cccgcggcat ggccaggtcg cgcaggttgg cctccacggc ctgggccagc
     3721 ccgccggccc cggtgcggcg ctgccggccg acggcgtcct cggcccgggc cagggcggcg
     3781 tcgacgtcct gccgctcggc ctcgagacgg gccaccttgg ccccgtggct ctcgaggtcg
     3841 gcgaggcggc gccgggtctc cgctgcgtag gcgacgatgt cggcgaccgt ggcgccgtac
     3901 ttgcggccca gctcgcggag gcgatgtcgg cgggaccgga cctcggccag ccggtcgggg
     3961 tcgtcctgga gccgctcggc ggcgtggcgc agctcggcgc ccacctcctg ggcggcggcc
     4021 accaggtccc gcagccgggt ctcggggccg gcgaggggcg cccggccggc cagcgcgccc
     4081 acggcctggc cgaggccgtc cagcacgccg ccatcgccgc tgacggcgtg ccgagcggcc
     4141 tcggccgcct cccggtgggc ggtggcggcc gagagggcgt cctcctcggc ctcgagccgg
     4201 acgtcctcgt cggggtcggc caggcccgcg ccgtcgatct cgtcgagctg gaagcgcagc
     4261 aggtccgtct ccctcgccct ggcccgggcg tcgccgccga gggaggccag ctgctcgtcg
     4321 atctccctcc ggcgggagcg agccgcggtc ctcggcccga ggtcgaccgc cgcgaaggcg
     4381 tcgagggcgt cccgctggct cgccgccccc agcagcgact ggtgggcgtg ctggccgtag
     4441 aggtcgacca ggcccgctcc caactcggcc agggcgccgg ccgtgcacat gcgcccgtcg
     4501 aggtagcccc gggagcgccc gcctccggac gggaccaccc gggccagcac gtgctcgtcg
     4561 tcgtggacga ggaaacgccc ctcgacgacc gcctcggccg ctccggggcg gacgagaacg
     4621 gagtcggccc ggccgccgac cagcagctct atggcttcga cgaccagcgt cttgcccgca
     4681 ccggtctcgc ccgtgatggc cgtcatgccg gggcccaggg cgagccgcag gtcggccagg
     4741 acaccgaggt cgtgcaccga gatctccgtg agcatcagcg gtcggccaga ccgaacttgg
     4801 ccttcaggat gtgccggaag tcgcgggccc cgaacgacac cagcttggcc ggagtggcgg
     4861 cgcggcacgt gaccgcatcg ccggccacga gctcgccggt ggaccgcccg tcgaggagca
     4921 gctcggcccg ggagccgtcg aggatatcga ggcggacctc ctcctcgggc tccagcacca
     4981 gggaccggtc gaagagcatg tggggcgaca ccggcgtgat caggatcgcc cggaggtggg
     5041 gtgagacgat cggtccccgg gccgagaggt tgtacgccgt cgagcccgtg ggcgtggcca
     5101 cgatcagacc gtcggcggcg taggtgagga aaggacggcc gtcgatgccg accgccacgt
     5161 tcacggtgtt gcccgaggtg cccttctcga cgacgacctc gttgagggcg agcatggcgg
     5221 tttcttcctc gtcgcggccg gcccgaagcc aacgggcctc gagggtcatg cgctcctcga
     5281 tggtgtgctc cccggcccag aaccgctcca gggcgagcgc caggtgcgcg ggctccacct
     5341 cggtcaggta gccgaggtgg cccaggttga cgccgagcac gggcacgccg tggggggcgg
     5401 cgagctccac ggcccgcagc atggtgccgt cgcccccgag gctgacggcc agagtggctc
     5461 cgtccaccag catgtcgtcg gccacgcccg cgtgctccag gccggcggtg gcggcgtcgc
     5521 gctccgagat gcggacgccg tggccccggc tgccgagcca gcccgcggtc tccaccgcca
     5581 ggcgcacggc ctccggccgg tcggggtgga tgacgaggac gacgtcaccc accggtgtca
     5641 cccatcgcgc tggcttcggc gaccgccgcc gcgatcctcg gccccgggtc atggcccgcc
     5701 ggcgcgggct ggacccaaag
//
LOCUS       DASWGG010000140         3639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3042727, whole genome shotgun sequence.
ACCESSION   DASWGG010000140 DASWGG010000000
VERSION     DASWGG010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3042727"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1063
                     /gene="ligD"
                     /locus_tag="VF954_02475"
     CDS             86..1063
                     /gene="ligD"
                     /locus_tag="VF954_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX9529975.1"
                     /translation="MAPLPPSLAPMLATAGALPGRDADWAFEIKWDGVRVLAYVDGRP
                     ATTTLRLESRNRIVITQRYPEIGDLPGAIAGHAAVLDGEAVAFDEEGRPSFSRLQRRM
                     HIADPREAATRTTSYPVTYEIFDLLWLDGEDLTGLPFTQRRARLDGLQLPAGRWQVPA
                     YHVGDGTALLAATRAQGLEGVVAKKLTSPYEPGRRSRNWVKVKNFRGQEVVIGGWLAG
                     SGGRAGRLGALLVGYHDEAGLLRYAGRVGTGFTDRVLRDLQGALEARAATASPFSDPI
                     PERAARFVRPELVAEVAFSEWTPDGLLRHPSYKGLRSDKDPREVVREPS"
     gene            1079..1945
                     /locus_tag="VF954_02480"
     CDS             1079..1945
                     /locus_tag="VF954_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX9529976.1"
                     /translation="MARPMWSGSISFGLVNVPVKLFTAVRKKDVHFNQLHATDGARIS
                     QRRVCSLDGEEVPWEEIAKGYEVAPGQYVMIEPAELDSLDPEATHTIDIEDFVALEGI
                     DPLFFDSSYYLVPDQRGAKAYRLLLDAMLESGQVGIARVVMRTKQYLCAVRPVGEALV
                     LTTMNFADEVVSRDELDDLPGEQSGASERELRMARQLIESLTTDFEPGRYSDTYRSEV
                     LALIERKAEGQEIVAPEAPEKPAPVIDLMAALEASLAAAKDSGKTAADVAGEGSGAGE
                     AKPGSSRRRRAS"
     gene            1952..2875
                     /gene="ligD"
                     /locus_tag="VF954_02485"
     CDS             1952..2875
                     /gene="ligD"
                     /locus_tag="VF954_02485"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX9529977.1"
                     /translation="MVPPAGGTRVDVDGHELTLTNLDKVLYPAAGTTKGEVIGYYQAI
                     APALLAHLAGRPLTLKRYPNGVDASYFYEKRCPSHRPGWLATVAVPSERSKADVPYCV
                     IENRAGLVWVANLASLELHPSLSMADRIEQPTTVVFDLDPGAPATIVECARVGLELRE
                     VFDQLGLECFAKTSGSKGMQLYLPLNSEVTYERTKPFALAVAQLLERRRPELVVSSMK
                     KELRTGKVLIDWSQNDEHKTTICVYSLRARPRPTVSTPLTWDEVEHVASTGEAASVEF
                     VAGEVVARVREMGDLFAPVATLEQELPDLLL"
     gene            3098..>3639
                     /locus_tag="VF954_02490"
     CDS             3098..>3639
                     /locus_tag="VF954_02490"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529978.1"
                     /translation="MAAGALLALSIPPWGFWPLALVGSGVLYAAVRGIGWRGRLAVGL
                     AAGLSQFLLGIFWIASFNLFGLVLLAALEGSFLAMACALTPAGRGRAAAWPAALAAAE
                     ALRGMWPWGGFPMGGLALGQAGSPLGPAARIGGDLLLVGLIGLAGTVLAEAASPAGRR
                     AAVTAGLVVVALAALGRVAP"
BASE COUNT          470 a         1353 c         1325 g          491 t
ORIGIN      
        1 aagcacgcct tttggtgcca agaatgcgcg ggcggggcgg ccacggcccc cggcccacgg
       61 cccccgggcg ggccacaatg agccggtggc gccgcttccg cccagcctgg ccccgatgct
      121 cgccaccgcc ggggcgctgc ccggccgcga cgccgactgg gccttcgaga tcaagtggga
      181 cggcgtgcgg gtcctggcct acgtcgacgg ccgccccgcc accaccacgc tgcgcctgga
      241 gagccgcaac cgcatcgtaa tcacgcagcg ctacccggag atcggggacc tgcccggcgc
      301 catcgccggc cacgcagccg tgctggacgg cgaggcggtc gccttcgacg aggagggccg
      361 gcccagcttc agccggctcc agcgccgcat gcacatcgcc gatccccggg aggccgcgac
      421 ccgcaccacg agctaccccg tcacctacga gatcttcgac ctgctgtggc tggacgggga
      481 ggacctgacc ggcctgccct tcacgcagcg gcgggcccgc ctcgacgggc tccagctgcc
      541 tgccgggcgc tggcaggtgc ccgcctacca cgtgggcgac gggaccgccc tgctcgccgc
      601 cacccgggcc cagggcctcg agggcgtggt ggccaagaag ctgaccagcc cctacgagcc
      661 ggggcggcgc tcccgcaact gggtcaaggt caagaacttc cgcggccagg aggtcgtcat
      721 cggcggctgg ctcgccggct ccggcggccg ggcgggacgc ctcggggcgc tgctcgtcgg
      781 ctaccacgac gaagccggcc tcctgcgcta cgcgggccgg gtcggcaccg gcttcaccga
      841 ccgggtgctg cgcgaccttc agggcgccct cgaggccagg gccgcgacgg ccagcccttt
      901 ctccgacccc atccccgagc gggccgcccg cttcgtgcgc cccgagctgg tggccgaggt
      961 cgccttctcc gagtggacgc ccgacgggct cctgcggcac ccctcctaca agggcctccg
     1021 gtcggacaag gacccccgcg aggtggtgcg ggagccgtct tagggggaga atggagccat
     1081 ggccagaccc atgtggagcg gctcgatcag cttcgggctg gtcaacgtcc cggtgaagct
     1141 gttcaccgcg gtgcgcaaga aggacgtgca cttcaaccag ctgcacgcca cggacggggc
     1201 gcggatctcc cagaggcggg tgtgctcgct ggacggcgag gaggtcccct gggaggagat
     1261 cgccaagggc tacgaggtcg cgcccggcca gtacgtgatg atcgagccgg ccgagctcga
     1321 ctccctcgac cccgaggcca cccacaccat cgacatcgag gacttcgtgg ccctggaggg
     1381 gatcgacccg ttgttcttcg actcgtccta ctacctggtc cccgaccaga ggggtgccaa
     1441 ggcctaccgc ctgctcctcg acgccatgct cgagtccggc caggtcggca tcgcccgggt
     1501 ggtgatgcgg accaagcagt acctctgcgc cgtgcggccg gtcggggagg ccctggtcct
     1561 cacgaccatg aacttcgccg acgaggtcgt gtcccgcgac gagctcgacg accttcccgg
     1621 cgagcagtcg ggcgcgtccg agcgggagct gcgcatggcc cgccagctga tcgagtcgct
     1681 caccaccgac ttcgagccgg ggcgctacag cgacacctac cgctccgagg tcctcgccct
     1741 catcgagcgc aaagccgaag gccaggagat cgtcgcgccc gaagcgccgg agaagccggc
     1801 cccggtcatc gaccttatgg ccgccctgga ggcctcgctg gccgcggcca aggactcggg
     1861 gaaaacggcg gccgacgtgg ccggcgaggg cagtggcgcc ggagaggcca agcccggctc
     1921 gtcccggcgg cgtagggcgt cgtaaacgac ggtggtcccg ccggcgggtg gcacgcgggt
     1981 cgacgtcgac ggccacgagc tgaccttgac caacctggac aaggtcctgt acccggccgc
     2041 cgggacgacc aagggcgagg tcatcggcta ctaccaggcc atcgcgccag ccctgctggc
     2101 ccacctggcc ggccggcccc tgacgctcaa gcggtacccg aacggcgtcg acgcctccta
     2161 cttctacgag aagcgctgcc cgtcgcaccg ccccggctgg ctggcgacgg tggcggtgcc
     2221 cagcgagcgc agcaaggccg acgtccccta ctgcgtgatc gagaaccggg ccgggctggt
     2281 gtgggtcgcc aacctggcca gcctcgagct gcacccttcg ctgtcaatgg ccgacaggat
     2341 cgagcagccc acgacggtgg tgttcgacct cgaccccggg gcgccggcca ccatcgtcga
     2401 gtgcgcgagg gtggggctgg agctgcggga ggtgttcgac cagctggggc tggagtgctt
     2461 cgccaagacc tccgggtcca agggcatgca gctgtacctc ccgctcaact ccgaggtcac
     2521 ctacgagcgg accaagccgt tcgccctggc ggtggcccag ctgctcgaga ggcgccgccc
     2581 cgaactggtg gtgtcgtcga tgaagaagga gctgcgcacc ggcaaggtgc tcatcgactg
     2641 gagccagaac gacgagcaca agaccaccat ctgcgtgtac tcgctgcggg cccggccccg
     2701 gccgaccgtg tcgacgccgc tcacatggga cgaggtcgag cacgtggcgt cgaccggtga
     2761 ggccgcctcg gtggagttcg tggccggcga ggtggtggcg cgggtgaggg agatggggga
     2821 cctgttcgcc ccggtggcca ccctcgagca ggagcttccc gacctcctcc tgtagcccat
     2881 ccagcccggc tggggcccca accccggcct cctccgcacg gcgggggccc cagccccgcc
     2941 ggcggccgtc cgctcatcca gcccgtccgg ggccccaacc ccggcctcct ccgcacggcg
     3001 ggggccccag ccccgccggc ggccgtcgct catctagctt cgggccatcg ctccgggctc
     3061 ccacctctgg tcgtggccgg cgcggctgcc ggcgggggtg gccgcgggcg ccctgctggc
     3121 gctgtccatc ccgccgtggg gcttctggcc cctggccctg gtgggctcgg gcgtgctcta
     3181 cgccgccgtg cggggcatcg gttggcgggg ccggttggcg gtcgggctgg cggccgggct
     3241 gagccagttc ctgctcggca tcttctggat cgcgagcttc aacctgttcg gcctggtcct
     3301 gctggccgcg ctcgaggggt ccttcctcgc catggcgtgc gccctgactc ccgccggccg
     3361 gggccgggcg gcggcctggc cggcggcgct ggcggccgcc gaggccttgc gggggatgtg
     3421 gccgtggggc gggttcccga tgggcgggct ggccctgggc caggccggct ccccgctcgg
     3481 gccggccgcc cgcatcggcg gcgacctgct cctcgtgggc ctgatcggcc tggccgggac
     3541 ggtcctggcc gaggccgcca gcccggcggg gcggcgcgcc gcggtgacgg ccgggctggt
     3601 ggtcgtggcc ctggcggccc tgggccgggt ggcgcccga
//
LOCUS       DASWGG010000141         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1112673, whole genome shotgun sequence.
ACCESSION   DASWGG010000141 DASWGG010000000
VERSION     DASWGG010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112673"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1208
                     /locus_tag="VF954_02495"
     CDS             216..1208
                     /locus_tag="VF954_02495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX9529979.1"
                     /translation="MSEKVKGGWQVEPRRTVTAGLRYLRRPDVRRAPARLVLRRADFE
                     IRRLIGGDRLGRPRTFSLPGGTRVTACLDDCVGRSLYIYGTYEWASARLLEDLLHPGG
                     VFVDVGAHVGSLALPAARRVGPEGLVVAFEPNGRAREQLVSGVRASRVANVVVLGVGL
                     ADRDLRAVLRPGGDGNSGMTRLIEAGGPAGRGRSGGGVECRTLDGIRPEIALPPVDVL
                     KVDVEGWEARVLQGARGLIADDRPAVVFEVNGLRADGGSWHSPAMDWLHEEGYALFGM
                     GPGGAAALRRLWPGEDPRPFEEPWLALNLVAFHPDRPLASFADADRRVLRAAAS"
     gene            complement(1123..>2231)
                     /locus_tag="VF954_02500"
     CDS             complement(1123..>2231)
                     /locus_tag="VF954_02500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9529980.1"
                     /translation="HGGAERVITAAARALPAQGLVPGVVLLAEGPLEDWLHAAGVPYR
                     VLRAPRTRQVHRAVPAVVRLSREWRRTGTDVVLSSLPKGHLYGGLAARLAGVPEVRWQ
                     HTFPGRSWFDRLADRVPAAATICVSHAVADRMKDPTEVVLPGIELAEVRSREGSGRAI
                     RDRLVPPGGWLVGVVSRVHRGKGLETFVEAAALVSARTPGARFVAVGGPHDAASEGLA
                     RQLRARAERLGSTMTFAGHQPDVFPWIDSLDLLVAPSTVEAFGLSVLEALALGTPVVA
                     TDSGGPAEIIGGSGAGCIVAVDEAGAMAAAISELLADPARRARMSELARARAGELSVE
                     AMAAGLATTLRRAARAGPRRRSWRAGGRDGRRRG"
BASE COUNT          297 a          838 c          840 g          256 t
ORIGIN      
        1 acgccggccg atccggccag cacggcgaac ggccggtccc acggccgggc ccacggccgg
       61 gcccaccaag acgcgggccg cgtcaccacc accggggcgc ccatcggtcg aaatgtaccc
      121 gcccttgccc cgacacgggg cgccgcagtg accggccgac gtttcgcacc gcaagttttg
      181 ccccactacc gagttctccc atcggtggag agaaggtgag cgagaaggtg aaggggggtt
      241 ggcaggtgga acccaggagg acggtgacgg cagggctgcg gtaccttcgg cgacccgacg
      301 tgcgccgggc ccccgcccgg ctggtgctgc ggcgggccga tttcgagatc cggcgcctga
      361 tcggcgggga tcggctcggc cggccacgga cgttctcgct gcccggcggg acgagggtca
      421 ccgcctgcct cgacgactgc gtgggccgct ccctctacat ctacggcacc tacgagtggg
      481 cctcggccag gctcctggag gacctgctcc acccgggcgg ggtcttcgtc gacgtcggcg
      541 cccacgtggg ctctctcgcc ctgcccgcgg cccgcagagt cgggcccgag ggcctggtgg
      601 tggccttcga gcccaacggc agggctcggg agcagctcgt ctcgggcgtc cgggccagca
      661 gggtggccaa cgtcgtcgtg ctcggtgtgg gactggccga tcgcgacctg cgggcggttc
      721 tgcgtcccgg cggcgacggc aactccggga tgacccgtct catcgaggcc ggcgggccgg
      781 ccggccgcgg ccggagcggc ggcggagtcg agtgccgcac cctcgacggc atccggcccg
      841 agatcgccct gcccccggtc gacgtcctca aggtcgacgt cgagggatgg gaggcccgag
      901 tcctccaggg cgccagaggg cttatcgccg acgaccgccc agccgtggtc ttcgaggtaa
      961 acgggctgcg cgccgacggg gggtcgtggc acagcccggc gatggactgg ctgcacgagg
     1021 agggctacgc cctgttcggg atgggccccg ggggcgcggc cgcgctgcgg cggctgtggc
     1081 ccggggagga cccgcggccc ttcgaggagc cctggctggc actcaacctc gtcgccttcc
     1141 atcccgaccg cccgctcgcc agcttcgccg acgcggaccg gcgcgtgctg cgcgccgcag
     1201 cgtcgtagcg agccccgccg ccatggcctc gaccgagagc tcaccggcac gggcccgcgc
     1261 cagctcgctc attcgcgccc ggcgcgccgg gtcggcgagc agctccgaga tggcggccgc
     1321 catcgccccg gcttcgtcga cggccacgat gcaccccgcc ccggatcccc cgatgatctc
     1381 ggctgggccg ccggagtcgg tggcgaccac gggcgtgccg agggccagcg cctcgagaac
     1441 ggacaggccg aacgcctcca cagtcgaggg cgccacgagc aggtcgagcg agtcgatcca
     1501 cgggaagacg tcgggctggt gaccggcaaa ggtcatcgtc gaccccaggc gctcggcacg
     1561 ggcccgcagc tgccgggcga gaccctcgga cgcggcgtcg tgcggtcccc cgacggcgac
     1621 gaaccgagca ccgggggtgc gcgccgacac cagcgcggcg gcctccacga aggtctccag
     1681 gcccttgccc cggtggacac gcgacaccac gccgacgagc cagccgcccg ggggcaccag
     1741 ccggtcacgg atggcccggc ccgagccctc ccgggaccgc acctcggcca gctcgatccc
     1801 gggcaggacc acctcggtcg ggtccttcat gcgatcggcc accgcgtggc tgacacagat
     1861 ggtggccgcc gcgggcacgc ggtcggcgag ccggtcgaac cagctacggc cggggaaggt
     1921 gtgctgccat cgcacctcgg gaacgccggc cagccgggcg gcgaggccgc cgtacaggtg
     1981 ccccttgggc aggctggaca gcacgacgtc ggtacccgtc cgccgccact ctcgggacaa
     2041 gcggacgacg gcgggcacgg cgcggtgcac ctggcgcgtc cgcggggcgc gcagcacgcg
     2101 gtaaggaacc ccggcggcgt ggagccagtc ctcgaggggg ccctcggcca gcaggacgac
     2161 gccggggacc aggccctgcg cgggcagggc gcgcgccgcc gccgtgatca cgcgctccgc
     2221 tccgccgtgg g
//
LOCUS       DASWGG010000142         1780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1429041, whole genome shotgun sequence.
ACCESSION   DASWGG010000142 DASWGG010000000
VERSION     DASWGG010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429041"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VF954_02505"
     CDS             complement(<1..287)
                     /locus_tag="VF954_02505"
                     /inference="COORDINATES: protein motif:HMM:NF019320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HEX9529981.1"
                     /translation="MEVGVRGIVFRLLEQAVVDEHGEEMWDTLLDVAQVSGAYTSLGV
                     YDDSELMALVGAASSAFGLDDPGVLRWLGRAALPMLARSYPDFFTPHTSAKA"
     gene            387..1496
                     /gene="prfB"
                     /locus_tag="VF954_02510"
     CDS             387..1496
                     /gene="prfB"
                     /locus_tag="VF954_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153759895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX9529982.1"
                     /translation="MRDFSPELADLRRRLEDARGYLQIDAARARLAELEKEASDPSLW
                     DDPDAARKVTTALARVKDDVDRVDGLERRLEDLETLDELAREEGDESLEGEIAAGVAA
                     LDGDLAALDLQALFTGEHDERDAICEIHSGAGGTDAADWADMMLRMYRRWAERRGFAM
                     EIDEVSPAEEAGISSATFIVKGRHAYGLLLAEKGVHRLVRISPFDSQARRQTSFASFD
                     AVPALDEAEEPEINPEDLRVDTYRSQGAGGQHVNVTDSAVRITHLPTGIVVSCQNERS
                     QHQNRAKAMQILGARLAELARQERRKELDALSGEKRDVAWGSQIRSYVLAPYQLVKDL
                     RTDYETGNVPAVLDGDLDPFMEAYLQWRRREGADS"
     gene            complement(1552..>1780)
                     /locus_tag="VF954_02515"
     CDS             complement(1552..>1780)
                     /locus_tag="VF954_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529983.1"
                     /translation="TTTAPRPIYANPDFLAANFADHIEPRRVSAVTPVGRPPSLPVMT
                     AFGLLALTGGGHAAVRLHSRRSPKARPTPPT"
BASE COUNT          278 a          649 c          635 g          218 t
ORIGIN      
        1 gccttggccg acgtgtgggg cgtgaagaag tcggggtacg accgggccag catgggcagg
       61 gccgcccggc ccaaccagcg cagcaccccg gggtcatcca gcccgaaggc cgatgacgcc
      121 gcgcccacga gcgccatgag ctcgctgtcg tcgtagacgc caagagacgt ataggcgccc
      181 gaaacctgcg cgacatcaag cagcgtgtcc cacatctctt cgccgtgctc gtcgacgacc
      241 gcctgctcca ggagcctgaa cacgatgccc cgcaccccga cctccaccac gcggcccctt
      301 gtggcggagc ataccgccgg gagccgctgc caccagcgaa gctgtagcct cgctggtcga
      361 cctgggacgg ataacgaggc ttctccgtgc gtgacttttc ccccgaactg gccgacctgc
      421 ggcgccgcct ggaggacgcc cgcggctacc tccagatcga cgccgcccgg gcccggctgg
      481 ccgaactgga gaaggaggcc agcgacccgt ccctgtggga cgaccccgac gccgcccgca
      541 aggtgaccac cgcgctggcc cgggtgaagg acgacgtcga ccgggtggac gggttggagc
      601 ggcgcctgga agacctcgag accctcgacg agctggcccg ggaggagggc gacgagtcgc
      661 tcgagggcga gatcgccgcc ggcgtcgccg ccctcgacgg ggacctcgcc gccctcgacc
      721 tccaggccct gttcaccggc gagcacgacg agcgcgacgc catctgcgag atccactccg
      781 gcgccggcgg caccgacgcc gccgactggg ccgacatgat gctgcgcatg taccggcgct
      841 gggccgagcg ccgcggcttc gccatggaga tcgacgaagt gtcccccgcc gaggaggcgg
      901 gcatcagcag cgccaccttc atcgtcaagg gccgccacgc ctacggcctc ctgttggccg
      961 agaaaggcgt gcaccgcctg gtgcggatct cgccgttcga ttcgcaggcc cggcgccaga
     1021 cgtcgttcgc ctccttcgac gccgtccccg ccctcgacga ggccgaggag cccgagatca
     1081 accccgagga cctccgggtc gacacctacc gttcccaggg cgccggcggc cagcacgtca
     1141 acgtgaccga ctcggcggtg cggatcaccc acctcccgac cggcatcgtc gtgtcgtgcc
     1201 agaacgagcg ctcccagcac cagaaccggg ccaaggccat gcagatcctc ggggcccggc
     1261 tggccgagct ggcccggcag gagcgccgca aggagctcga cgccctgtcg ggggagaagc
     1321 gcgacgtcgc ctggggcagc cagatccggt cctacgtcct cgccccctac cagctggtga
     1381 aggacctccg caccgactac gaaaccggca acgtcccggc cgtgctcgac ggcgacctcg
     1441 accccttcat ggaggcctac ctccagtggc ggcgccggga gggtgccgac agttagaccg
     1501 ccaagccccg gaggaaggcc ggccgcggcg ggttgacctg gtcggggtgg gtcaggttgg
     1561 aggcgtgggc cgcgccttcg gcgaccggcg gctgtggagg cgcaccgcgg cgtggccgcc
     1621 tccggtcagg gccagcaggc cgaaggcggt catgacgggg agggacggcg ggcggccgac
     1681 cggggtgacg gccgacaccc gcctcggctc gatgtggtcg gcgaagttgg cggccaggaa
     1741 gtcggggttg gcgtagatcg gccggggggc cgtggtcgtg
//
LOCUS       DASWGG010000143         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1116247, whole genome shotgun sequence.
ACCESSION   DASWGG010000143 DASWGG010000000
VERSION     DASWGG010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116247"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VF954_02520"
     CDS             <1..411
                     /locus_tag="VF954_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529984.1"
                     /translation="FGDPFLPLSVQEERAHRGGFANPFSSLDRAVRDLSGGVHIGTGL
                     HLPWAVLMVGLAVVAARRWPPAYGAFAVAVVVVALSAANLDSAERYALSAFPLVLAGA
                     GLAASERVFRAVLALSSALLVAYATLAFLNLSVP"
     gene            408..1709
                     /locus_tag="VF954_02525"
     CDS             408..1709
                     /locus_tag="VF954_02525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9529985.1"
                     /translation="MTPAGATRRRLTGFHLGLLAISLAGFGVRFVYVWFFHRHIPVGG
                     DSYFYHYGANLLVDGHGFIGPEQYLLGHIVFPSAAHPPLYLLTLAAASLVGLRSYTAH
                     QLVSCAIGAGTVAMVGLAARETAGSRRAGLVAAALAAVYPFMWIIDGAVMSETDMLFT
                     AALVVYLAYRMWHAPSPGRAVALGVAVGLAALTRAEEALLAALVLVPLALWLRGPEMR
                     RRLQLAGIGVLATVVTVAPWCAYNLSRFNQPELLSTGFGPVLAVSYCDNTFYGQYTGW
                     WWLECDRRLPAPVRDESDQDVYLRRAALTYAGHHVRRLPVVMFARLGRTFAFYRAGHQ
                     IFDDQIEQRPLVDSQWGLALYYAMIGPAAYGAVALRRRRQPLAPLLGLVASVVVVGAF
                     VYGNTRFRAPADVALVILAGVGVDTLLSGRARRADEPGQPA"
BASE COUNT          239 a          821 c          716 g          390 t
ORIGIN      
        1 ttcggcgacc cgttcctgcc cctctcggtg caggaggagc gcgcccaccg gggcgggttc
       61 gccaacccct tttcctcgct cgaccgggcc gtgcgcgacc tttccggcgg cgtgcacatc
      121 ggcaccgggc tgcacctgcc gtgggcggtc ctcatggtcg gcctggcggt ggtggccgcc
      181 cggcgctggc cgcccgccta cggcgcgttc gccgtggccg tggtggtcgt cgccctgtcc
      241 gcggccaacc tggactcggc cgagcgctac gccctgtcgg ccttcccgct ggtgctggcc
      301 ggcgccggcc tggccgcctc cgagcgcgtc ttccgcgccg tcctcgccct gtcgtccgcc
      361 ttgctggtgg cctacgccac gctggccttc ctcaacctgt ccgtgccatg acacctgcgg
      421 gtgccacgcg gcgtcgcttg accggcttcc acctgggcct gctcgccatc agcctggcgg
      481 gcttcggcgt gcggttcgtc tacgtctggt tcttccaccg ccacataccc gtcggcggcg
      541 actcgtactt ctaccactac ggcgccaacc tgctggtcga cggccacggc ttcatcgggc
      601 ccgagcagta cctgctcggg cacatcgtct tcccgtcggc ggcccatccc ccgctctacc
      661 tgctgaccct cgccgccgcc tccctggtcg ggctgcgctc ctacacggcc caccagctgg
      721 tgtcgtgcgc cataggggcc gggacggtgg ccatggtggg cctggctgct cgagagacgg
      781 ccggctcccg gcgggccggg ctcgtcgccg ccgccctggc cgccgtctac cccttcatgt
      841 ggatcatcga cggcgccgtc atgtcggaga cggacatgct cttcacggcc gccctggtgg
      901 tgtacctggc ctaccgcatg tggcacgcgc cctcccccgg ccgggccgtc gccctcgggg
      961 tcgccgtggg cctggccgcc ctgacccggg ccgaggaggc cctgctggcg gccctcgtcc
     1021 tcgttcctct cgcgctgtgg ctgcgcggcc ccgagatgcg ccgtcgcctg cagctggcgg
     1081 gcatcggcgt gctcgccacc gtcgtgaccg tggccccgtg gtgcgcgtac aacctgagcc
     1141 gcttcaacca gcccgagctg ctctcgaccg ggttcgggcc ggtgctcgcg gtgtcgtact
     1201 gcgacaacac cttctacggg cagtacacgg gctggtggtg gctggagtgt gaccgccggc
     1261 tgccggcccc ggtgcgggac gagtccgacc aggacgtgta cctgcggagg gccgcgctca
     1321 cctacgccgg ccaccacgtg cgccggctgc cggtggtgat gttcgcccgg ctggggcgca
     1381 ccttcgcctt ctaccgggcc ggccaccaga tcttcgacga ccagatcgag cagcgtccgc
     1441 tcgtcgactc ccagtggggg ctcgccctgt actacgccat gatcgggccc gcggcgtacg
     1501 gcgccgtcgc cctgcgccgg cgtcgccagc cgctcgcacc cctcctcggg ctggtggcct
     1561 cggtggtggt ggtgggcgcc ttcgtgtacg gcaacacccg cttccgtgcc ccggcggacg
     1621 tcgccctcgt catcctcgcg ggcgtggggg tagacaccct gctctctggg cgcgcccggc
     1681 gcgccgatga gccgggccaa cctgcttgag actcacctcc tatggacatt ttcatgtcat
     1741 ttcattttac tggggagata agacggtctg agaccaccaa atctccccag ctacgcctca
     1801 ccgaactggc cgccgccctc gaacgcgggg cgtgagggcg gccggcggtc tagcactact
     1861 cccaatggag cccgccttca tcagagcgcg taaccggcgg ccaacctcca atccctcgcc
     1921 tgggatcggg gcgtgtgggg ccaagcgcca accgccaact acttgcgcgg tgcaactcca
     1981 agctcccggt cggacaattg gcttcgccca ctcggccgcg caagtagtca ttcctgaagg
     2041 agcgctcttt gtcaatacag cgccggccct tcgggccggc tcctttgggt gttgagcgct
     2101 tgtcgcgatg gccgaatttg agcaacgcgg gcgcgtcggg ttagtcccgg agttggagcc
     2161 gagagg
//
LOCUS       DASWGG010000144        10956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_391551, whole genome shotgun sequence.
ACCESSION   DASWGG010000144 DASWGG010000000
VERSION     DASWGG010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10956
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391551"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /gene="secF"
                     /locus_tag="VF954_02530"
     CDS             complement(<1..1177)
                     /gene="secF"
                     /locus_tag="VF954_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX9529986.1"
                     /translation="MSTAKRGLWTRLYHGETAFDFIGRKNQWFALSGAVILIGLASLL
                     VQGLNLGIDFRGGTAWSFRDTHATVAGVRGVMAQQGLGDATVQLFSSGTVTVQADLKG
                     KSVTDRQGIVAAVQDKLAGYAKVTHDAVSVNDVGPTWGHDITAKAERALALFFVAIVI
                     YISLRFEWKMALAAIVAVVHDILVTVGIYSLFGFPVTPATVVAFLTILGYSLYDTIVV
                     FDKVEENTRGLASTGRLTYTDTVNLSMNQVLMRSINTSFVAILPILSILVIGAYILGA
                     ATLKEFGLALFVGLTTGAYSSIFIASPLLAVLKEREPRYATIRQRLASRGATGSLLTP
                     AAAAAAAGAAAAGGGRSTTLLTPKAPRDAVVDDDLGVATAAAPGPPPRPRQGPRPGGG
                     "
     gene            complement(1174..2661)
                     /gene="secD"
                     /locus_tag="VF954_02535"
     CDS             complement(1174..2661)
                     /gene="secD"
                     /locus_tag="VF954_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX9529987.1"
                     /translation="MRRSLTVSLLGVVAVAIVALVATVASGNSPRLGLDLEGGVSVVY
                     APEHPVPTGVLDQAIAIIRNRVDALGVAEPDITRQGQNIVVQLPGVKNPDRVVQTIGT
                     TAELYFRPVIGNQPYNTGATTTTAPKATTTTVAGATTTAGPSTSTTAAGATTTTTAPL
                     GCATPSGGDDPAQQVIIPDKAHTQCYVLAKTYTDASGNQLTGRALSGADAVIDPQSGQ
                     WLVSFTLNGSGSKLFDEMAAANFKKPVAIDLDGEVESAPEINAQSFGGKGQITGTFTS
                     RQAKDLALVLRYGSLPVKLLQQDRSTVSATLGKDSLRAGLVAGIAGLVLVLLYMIFYY
                     RALGIVVLLGLSVSAMLLYSIIVWLGQSNGLALSLAGATGVIVSVGVTVDSYVVYFER
                     LKDEIRSGKTVRSSVDRGFARAYRTIVAADLVSLIAAVLLYLLTVGAVRGFAFFLGLS
                     TLLDMVTAYVFTRPMVVLLGRNRIFTEARWLGVARGLAASTGADR"
     gene            complement(2661..3029)
                     /gene="yajC"
                     /locus_tag="VF954_02540"
     CDS             complement(2661..3029)
                     /gene="yajC"
                     /locus_tag="VF954_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX9529988.1"
                     /translation="MPNLIFLMLIAVVFYTLLVRGPRQRARRRQEVVAAMGVGDEVVT
                     IGGIVGRIAAVDGDRVALEVAPGVTLTFLRAGINRRIEPVDDSPGESGDAVDLTGGGA
                     DLDASGDDDLGGGDLRDGTA"
     gene            complement(3115..4209)
                     /gene="tgt"
                     /locus_tag="VF954_02545"
     CDS             complement(3115..4209)
                     /gene="tgt"
                     /locus_tag="VF954_02545"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX9529989.1"
                     /translation="MTAQLTVDGRDGPARHGRVSTSRGAFSVPCFMPVGTRGSVRTLS
                     AADLDDLGVGVVLANAYHLMLRPGAATVAALGGLHRFTGWEGHVLTDSGGYQVFSLAP
                     AVDDDGVTFRSSYDGSTHRLTPEAAVAVQEALGGDIQMVLDVCSPLPAPAEVLRLAVE
                     RTAAWAARAKRAHHLPGQALFGIVQGGVDPDLRRESAQRTVEIGFDGYGIGGLSVGES
                     RPEMLEALAAAVEELPADRPRYLMGVGDPVGLVEAVALGVDMFDCVLPTRLARHGTVL
                     TSAGRFHVRNAALARDDGPLDPSCGCPVCRRWTRAYLRHLHMVGEPSAARLITLHNLR
                     WLLELVRRVSGAIEAGRLAQLRREVAEVWG"
     gene            complement(4206..5249)
                     /gene="queA"
                     /locus_tag="VF954_02550"
     CDS             complement(4206..5249)
                     /gene="queA"
                     /locus_tag="VF954_02550"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX9529990.1"
                     /translation="MAMDRYDYELPEAAIAQTPREPRHDARLLVATDPGGAVDHLRVR
                     DLAGLVRAGDVIVVNDTKVLPARLRLHKITGGAVEVLLLEPTGRAPEQWHALVRPGRR
                     VPAGTTLVAGERPALTVGGRPGAEDEDEDEEADADGRRVVTLLDPALPEQWGQVALPP
                     YIHRPLEDPGRYQTVYAAHPGAVAAPTAGLHLTAQVLDGCRRAGAHLMTVDLTVGLGT
                     FRPVTAERPEDHRVHTEAYAVPESTLDACRQARSDGRRVIAVGTTTVRALESAAATGQ
                     AAGRTALFIHGDYPFRVVDVLLTNFHLPRSSLLLLLEAFCGPRWRELYAVALGRGYGF
                     LSFGDAMVVARRA"
     gene            complement(5262..6350)
                     /gene="ruvB"
                     /locus_tag="VF954_02555"
     CDS             complement(5262..6350)
                     /gene="ruvB"
                     /locus_tag="VF954_02555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX9529991.1"
                     /translation="MSPRHDSLASPARAVEPAAEPIEAAEEVGLRPRHLGEFVGQPQL
                     KEHLDIVLQAARRRGQPADHVLLAGPPGLGKTSMAGIVATEMGVGLRITSGPALVRAG
                     DLAAILTDLREGDVLFIDEIHRLGRAVEEILYPAMEDFKLDILIGKGPTARAIRLDLP
                     PFTLVGATTRTGLITGPLRDRFGFVARLDYYSAEDLEAIVVRAASILGVHLERGGARE
                     IARRARGTPRIANRLLKRVRDFAEIRADGRVTEETASAGLALFGVDDLGLDKVDRAIL
                     AAVCERFGGGPVGLSTLAVSVGEEPETVEDVYEPYLLQRGLLMRTPRGRVATPQAWHH
                     LGLAAPRREPEPGLFDDPPEPEPRPPGA"
     gene            complement(6347..6973)
                     /gene="ruvA"
                     /locus_tag="VF954_02560"
     CDS             complement(6347..6973)
                     /gene="ruvA"
                     /locus_tag="VF954_02560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX9529992.1"
                     /translation="MIGSLRGILLDRSPRGDVLVEVGAVGYRVVVPTGTSATMGELGS
                     PVFLHVHTHVREDAIILYGFATREERACFEALIGAHGVGPGLALAILSVHSPAGLRRA
                     LLTDDADALTLVPGVGKKTAARLLMELKSRLDLPELDSGLVSVGVGPGGGSIDGGSLD
                     GGRSEVRAALAGLGYAADEVRDAVRELPDDGEVSELLRLALRSLASNR"
     gene            complement(6970..7491)
                     /gene="ruvC"
                     /locus_tag="VF954_02565"
     CDS             complement(6970..7491)
                     /gene="ruvC"
                     /locus_tag="VF954_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX9529993.1"
                     /translation="MFVLGIDPGLSRCGYGVVRRDSSSTLHAVAAGVITTAPDTPLAE
                     RLALLQREITGLIAECRPAAVVVERVFFQTNARTAMSVGQASGLALAAAASAGCEVVQ
                     YTSNEVKQAVVGYGSATKDQVQRMVQAILRLPERPRPPDAADALALAICHQTLLPLAR
                     RVAAARAAQGARS"
     gene            complement(7572..8000)
                     /locus_tag="VF954_02570"
     CDS             complement(7572..8000)
                     /locus_tag="VF954_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HEX9529994.1"
                     /translation="MQPGPWLIDKSALVRLGSSPEAEEWAERIERGLVRITTITLLEV
                     AYSARSAPDMRAGLTGAPIASMPIEYLTPAIEDRALAVITALAERGEHRGPSLPDLLL
                     AATAELSRLTVLHLDKDFELIAQVTGQPVERLDADTASDT"
     gene            complement(8021..8242)
                     /locus_tag="VF954_02575"
     CDS             complement(8021..8242)
                     /locus_tag="VF954_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX9529995.1"
                     /translation="MSDILIRDVPDADVAALDAHAAALGLSRNEYLRRRLHQDARHRA
                     VSVADTDLRRFATLFADLADREVMARAWS"
     gene            complement(8287..9036)
                     /locus_tag="VF954_02580"
     CDS             complement(8287..9036)
                     /locus_tag="VF954_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9529996.1"
                     /translation="MSGHSKWATIKHKKGAQDKARGKLFAKLIRQIEVAAREGGGDLT
                     SNATLRSMFQKARDASVPTDTIDRAIKRGTGELEGVRYESISYEGYAPAGVALIVETL
                     TDNRNRTGADVRSLLTRSGGSMAEPGAVSWQFERKGAIILPKDVSEDDLMLVALEAGA
                     EDLSDQGDTWQLTSPPAELNPLRGALEAAGIAFDSADLTMLPTTTVALDNEGDARKVL
                     RLVDALEDLDDVQNVFANFDIPDEILDGVET"
     gene            complement(9040..9624)
                     /gene="pdxT"
                     /locus_tag="VF954_02585"
     CDS             complement(9040..9624)
                     /gene="pdxT"
                     /locus_tag="VF954_02585"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX9529997.1"
                     /translation="MKVGILALQGDVREHARVLADLGVDTVEVTSPGELGGVDALVLP
                     GGESTTMSMLLESSGLFDALAERLGDGLAAFGTCAGMILLASTVVDGRDDQRCLGAVD
                     LVVRRNAFGRQVDSFETDLPVDGVAGGPVPAVFIRAPVVERAGPAVDVLATVARPDGV
                     RQPVLCRQGRVLVAAFHPELSGDCRLHELFLRGV"
     gene            complement(9679..10590)
                     /gene="pdxS"
                     /locus_tag="VF954_02590"
     CDS             complement(9679..10590)
                     /gene="pdxS"
                     /locus_tag="VF954_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX9529998.1"
                     /translation="MTDPARTTDGPARRTGTERVKRGLAEMLRGGVIMDVVTPEHAKI
                     AEDAGAVAVMALERVPADIRRDGGVARMSDPALIEGIKDTVTVPVMAKARIGHFAEAQ
                     VLQALGVDYIDESEVLTPADEAHHIDKWPFAVPFVCGATNLGEALRRISEGAAMIRSK
                     GEAGTGNIVEAVRHLRAILGDIRKVTQADGAELYAWAKALQAPVGLVAEIAETAGLPV
                     PLFCAGGIATPADAALVMQLGAQAVFVGSGIFKSGDPARRARAIVEATTHYRDPAILA
                     KVSKGLGEPMVGIEIDQVDVRLSERGW"
     gene            complement(10650..>10956)
                     /locus_tag="VF954_02595"
     CDS             complement(10650..>10956)
                     /locus_tag="VF954_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9529999.1"
                     /translation="VVGTMPGGRAALAAAGAWRSSPPSRASRREVAADLAGVALTRYP
                     PGLLAALEKVQVGPAGPAGVPARDAHLWLRPPEARVGEPAAVGPAPLAQRIETLREL"
BASE COUNT         1543 a         3926 c         4049 g         1438 t
ORIGIN      
        1 ggcccccacc ggggcggggc ccctggcgcg gccggggtgg gggcccgggg gcagccgccg
       61 tggccacgcc caggtcgtcg tcgaccaccg cgtcgcgggg ggctttcggg gtgagcaagg
      121 tcgtggaccg cccgccgccc gccgcggccg cgccggccgc cgccgcggcc gccgcggggg
      181 tcagcaggct gccggtggcg ccgcgcgagg ccagccgctg gcggatggtg gcgtagcggg
      241 gctcccgctc cttcagcacg gcgagcagcg gcgaggcgat gaagatggac gagtaggccc
      301 cggtcgtgag acccacgaag agggccagcc cgaactcctt gagcgtcgcc gccccgagga
      361 tgtaggcgcc gatgacgagg atcgacagga tgggcaggat ggccacgaac gaggtgttga
      421 tcgaccgcat cagcacctgg ttcattgaca ggttgacggt gtcggtgtag gtgagccggc
      481 cggtcgaggc cagcccccgg gtgttctcct ccaccttgtc gaagaccacg atggtgtcgt
      541 acagggagta gcccaggatg gtcaggaagg cgacgaccgt ggccggcgtg acggggaacc
      601 cgaacaggga gtagatcccg acggtcacca ggatgtcgtg gacgaccgcc acgatggccg
      661 ccagggccat cttccactcg aagcgcaggc tgatgtagat gacgatggcc acgaagaaca
      721 gggccagggc ccgctcggcc ttggccgtga tgtcgtggcc ccaggtcggg cccacgtcgt
      781 tgacgctgac cgcgtcgtgg gtgaccttgg cgtagccggc cagcttgtcc tggaccgcgg
      841 cgacgatgcc ctggcggtcg gtcaccgact tgcccttgag atcggcctgg acggtgaccg
      901 tccccgagct gaagagctgc acggtggcat cgcccaggcc ctgctgggcc atcacgcccc
      961 ggactccggc cacggtggcg tgggtgtcgc ggaacgacca ggccgtgcca ccccggaagt
     1021 cgatgcccag gttgagcccc tgcaccagca gcgaggccag cccgatcagg atcaccgcgc
     1081 cggacagcgc gaaccactgg ttcttgcggc cgatgaagtc gaaggccgtc tcgccgtggt
     1141 acagccgcgt ccacagaccc cgtttcgcgg tgctcatctg tcggctcccg tgctggccgc
     1201 cagcccccgg gcgaccccga gccagcgggc ctcggtgaag atgcggttgc gacccagcag
     1261 cacgaccatg gggcgggtga aaacgtacgc cgtgaccatg tcgagcaggg tggacaggcc
     1321 caggaagaag gcgaacccgc ggaccgcccc gacggtcagc aggtagagca gcacggccgc
     1381 gatgagcgac accaggtcgg cggccacgat agtgcgatag gcccgggcaa agccccggtc
     1441 gaccgacgag cggacggtct ttcccgatct gatctcgtcc ttgagccgct cgaagtacac
     1501 gacgtaggag tccacggtga cgcccacgga cacgatcacc cccgtggcgc cggccaggga
     1561 cagggccagg ccgttgctct gccccagcca cacaatgatg gagtacagca gcatggcgga
     1621 cacggacagg ccgaggagca ccacgatgcc gagggcgcgg tagtagaaga tcatgtacag
     1681 cagaacgagg accaggccgg cgatgccggc gaccagaccg gcccgcagcg agtccttgcc
     1741 gagcgtcgcc gacaccgtgg agcggtcctg ctggagcagc ttgacgggga gcgagccgta
     1801 gcgcagcacg agggcgaggt ccttggcctg gcgggaggtg aaggtgccgg tgatctggcc
     1861 cttgccgccg aagctctggg cgttgatctc cggcgccgac tcgacctcgc cgtcgaggtc
     1921 gatggccacc ggcttcttga agttggccgc cgccatctcg tcgaacagct tcgaccccga
     1981 gccgttgagg gtgaagctga ccagccactg gccgctctgg ggatcgatca ccgcgtcggc
     2041 gccggacagg gcccggccgg tcagctggtt gccgctcgcg tcggtgtagg tcttggccag
     2101 gacgtagcac tgggtgtggg ccttgtccgg gatgatgacc tgctgagccg ggtcgtcccc
     2161 cccggacggg gtggcgcacc cgaggggcgc cgtcgtggtg gtggtcgccc cggcagccgt
     2221 ggtggaggtg ctcggccccg cagtcgtcgt ggcaccggcg acggtggtcg tggtggcctt
     2281 tggcgcggtc gtggtggtgg cccccgtgtt gtacggctgg ttgccgatca ccggccggaa
     2341 gtagagctcg gcggtggtgc cgatggtctg gaccacccgg tcggggttct tcacgccggg
     2401 cagctgcacg acgatgttct ggccctggcg ggtgatgtcg ggctcggcca cgccgagggc
     2461 gtcgacccgg ttgcggatga tggcgatggc ctggtccagg accccggtcg gcacgggatg
     2521 ctcgggcgcg tacaccaccg acacgccgcc ctcgaggtcg aggccgagac ggggactgtt
     2581 gccggaggcg acggtggcga ccagcgccac gatggcgacc gccaccacgc cgagcagcga
     2641 cacggtgagc gagcgtcgca tcaggccgtt ccgtcgcgga ggtcgccgcc gcccagatcg
     2701 tcgtccccac tggcgtccag gtcggcgcca cctcccgtga ggtccacggc gtcgccactt
     2761 tccccagggc tgtcgtccac gggctcgatc ctgcggttga tgcccgcccg caggaacgtc
     2821 agggtcacgc ctggggccac ctcgagggcg acgcgatcac cgtccacggc ggcgatgcgg
     2881 cccacgatgc cgccgatggt cacgacctcg tcgcccacgc ccatcgccgc caccacctcc
     2941 tggcgccggc gggcccgctg gcgcgggccg cggaccagca gcgtgtagaa caccacggcg
     3001 atcagcatca ggaagatcag gttgggcatg cggcgttcgg gcctttcggg gtccgagggg
     3061 cgaccgacga cgttagcgcc aagcgcagcg ccatcgcccg cctggggcag cccttcaccc
     3121 ccacacctcg gccacctccc gtcgcagctg cgccagccgg cccgcctcga tcgcccccga
     3181 cacccgccgc accagctcca gcagccagcg caggttgtgc agcgtgatca ggcgggccgc
     3241 gctgggctcg cccaccatgt gcagatgccg caggtaggcc cgcgtccagc gccggcacac
     3301 cgggcagccg cacgacgggt ccagcgggcc gtcgtcgcgg gccagggcgg cgttgcggac
     3361 gtggaaccgc cccgccgagg tcagcacggt gccgtgacgg gccagccggg tgggcagcac
     3421 gcagtcgaac atgtcgaccc ccagggcgac ggcctcgacc aggcccacgg ggtccccgac
     3481 acccatcagg tagcggggcc ggtcggcggg cagctcctcg acggcggcgg ccagggcctc
     3541 gagcatctcc gggcgcgact ccccgaccga caggccaccg atgccgtagc cgtcgaagcc
     3601 gatctccacg gtccgttggg cgctctcccg gcggaggtcg gggtcgacgc cgccctgcac
     3661 gatcccgaac agggcctggc ccggaaggtg gtgggcccgc ttggcccggg ccgcccacgc
     3721 cgcggtgcgc tcgaccgcca gccgcagcac ctcggcgggg gcgggcagcg ggctgcacac
     3781 gtcgagcacc atctggatgt caccgcccag cgcctcctgc acggccaccg cggcctccgg
     3841 cgtgagccgg tgggtcgacc cgtcatagct cgaccggaac gtgaccccgt cgtcgtcgac
     3901 ggccggcgcc aggctgaaga cctggtagcc gcccgagtcg gtcaggacgt ggccctccca
     3961 gcccgtgaag cggtggagac cgcccagggc ggcgacggtg gcggccccgg gccgcagcat
     4021 caggtggtag gcgttggcca gcaccacgcc gacgccgagg tcgtccaggt cggcggcgga
     4081 cagcgtgcgg accgagccgc gcgtcccgac cggcatgaag cacgggacgg agaaagcccc
     4141 acgcgacgtg ctcacccggc cgtggcgggc gggaccgtcg cgcccgtcga ccgtcagctg
     4201 ggccgtcatg cccggcgggc gacgaccatg gcgtcgccga acgacaggaa gccgtacccc
     4261 cggcccagcg cgacggcgta cagctcccgc caccggggcc cgcagaacgc ctcgaggagc
     4321 agcagcagcg acgagcgcgg caggtggaag ttggtgagca gcacgtccac cacccggaac
     4381 gggtagtcgc catggatgaa cagggcggtc cgcccggccg cctgcccggt ggcggcggcc
     4441 gactcgaggg cccggacggt ggtcgtcccc acggcgatga cccggcggcc gtcggagcgg
     4501 gcctggcggc acgcgtcaag ggtcgactcg gggacggcgt acgcctcggt gtgcacacgg
     4561 tggtcctcgg gtcgctcggc ggtcacgggc cggaaggtgc ccagcccgac ggtgaggtcc
     4621 acggtcatga gatgcgcccc cgcccggcgg cagccgtcca ggacctgtgc ggtcaggtgg
     4681 agcccggcgg tgggcgcggc caccgcgccg gggtgggcgg catagacggt ctggtagcgg
     4741 ccggggtctt cgaggggccg gtggatgtag ggaggaagcg ccacctgtcc ccactgctcg
     4801 ggcagggcgg ggtccaggag ggtcacgacc cggcggccgt ccgcgtccgc ctcttcgtct
     4861 tcgtcttcgt cttcggcgcc cggccggccg ccgaccgtga gggcggggcg ctcacccgcg
     4921 accagggtgg tgcccgcggg gacgcggcgg cccggtcgga cgagggcatg ccattgctcc
     4981 ggggcccggc cggtgggctc gaggagcagg acctcgacgg cgccgcccgt gatcttgtgg
     5041 aggcgcagcc gggcgggcag gaccttggtg tcgttgacga cgatcacgtc gccggcccgg
     5101 accaggccgg cgaggtcccg cacgcgcagg tggtccacgg ctccgccggg gtcggtggcg
     5161 accaggagcc gggcgtcgtg gcggggctca cggggggtct gggcgatggc cgcctcgggc
     5221 agctcgtagt cgtagcggtc catggccatc gccgcgccag gttaagcgcc gggcggtcgc
     5281 ggctcgggct ccggcgggtc gtcgaacagt cccggctcgg gctcccgacg gggcgcggcc
     5341 aggccgaggt ggtgccaggc ctggggggtg gccacccggc cccggggggt gcgcatgagc
     5401 aggcctcgct ggaggaggta gggctcgtag acgtcctcca cggtctcggg ctcctccccc
     5461 acgctcacgg ccagggtgct gaggcccacg ggcccgccgc cgaagcgctc gcacacggcg
     5521 gccaggatgg cgcggtccac cttgtcgagc ccgagatcgt cgacgccgaa gagggccagg
     5581 ccggccgagg cggtctcctc cgtcacacgc ccgtcggccc ggatctccgc gaagtcccgg
     5641 acccgcttga gcagccggtt ggcgatgcgg ggcgtgcccc gcgcccggcg ggcgatctcc
     5701 cgggccccgc cccgctccag gtgcacgccc aggatggacg cggcccgcac gactatggcc
     5761 tcgaggtcct cggccgagta gtagtcgagc cgggccacga acccgaagcg gtcccgcagc
     5821 ggccccgtga tgagaccggt gcgggtcgtg gcccccacca gggtgaaggg cggcaggtcc
     5881 aaccggatgg cccgggccgt ggggcccttg ccgatcagga tgtcgagctt gaagtcctcc
     5941 atggccgggt agaggatctc ctccacggcc cggccgagcc ggtggatctc gtcgatgaac
     6001 agcacgtcgc cctcgcgcag gtcggtgagg atggcggcca ggtccccggc ccgcacgagc
     6061 gccggccccg aggtgatccg caggccgacg cccatctcgg tggcgacgat ccccgccatg
     6121 ctcgtcttgc cgagcccggg cggaccggcc agcagcacgt ggtcggcggg ctggcccctc
     6181 cgccgggcgg cctggagcac gatgtcgaga tgctccttga gctggggctg gccgacgaac
     6241 tcgccgagat ggcggggccg cagccccacc tcctcggccg cctcgatggg ctccgcggcc
     6301 ggctccacgg cccgggcggg gctggccagg ctgtcgtggc gggggctcac cggttgctcg
     6361 ccagggagcg cagcgccagg cggagcagct cggagacctc gccgtcgtca ggcagctcgc
     6421 ggacggcgtc gcgaacctcg tccgcggcgt agcccaggcc ggcgagggcg gcccggacct
     6481 cggagcgacc cccgtcgaga gagccgccgt cgatggagcc gccacccggg ccgaccccga
     6541 ccgacacgag cccggagtcc agctcgggca ggtcgagacg ggacttcagc tccatgagga
     6601 ggcgcgccgc ggtcttcttg cccacgcctg gcaccagggt cagggcgtcg gcgtcgtcgg
     6661 tgagcaaggc ccggcgcagc cctgccggcg agtggaccga gaggatggcg agcgccaggc
     6721 ccggcccgac cccgtgggcg ccgatgaggg cctcgaagca ggctcgctcc tcgcgggtgg
     6781 cgaacccgta aaggatgatg gcgtcctccc ggacgtgggt gtggacgtgg aggaacacgg
     6841 gcgagcccag ctcgcccatc gtcgccgagg tgcccgtcgg caccaccacc cggtagccca
     6901 cggccccgac ctcgaccagc acgtcaccac gtggggagcg gtcgagcagg atcccgcgta
     6961 gcgaaccgat catgaccggg ctccttgcgc tgctcgggcc gcggccaccc gtcgggcgag
     7021 cggcagcagg gtctgatggc agatggccaa cgccagggcg tcggccgcat ccggtggcct
     7081 gggccgctcg ggcagccgga ggatggcctg gaccatgcgc tggacctggt ccttggtcgc
     7141 cgacccgtag ccgaccacgg cctgtttgac ctcattggac gtgtactgca cgacctcgca
     7201 gcccgcgctg gcggccgccg ccagggccag gccgctggcc tggcccacgc tcatggcggt
     7261 gcgggcgttg gtctggaaga acacgcgctc gaccaccacc gcggccggtc ggcactcggc
     7321 gatcagcccg gtgatctccc gctggaggag agccagccgc tcggcgagcg gcgtgtccgg
     7381 cgccgtcgtg atcacgcccg cggccacggc gtggagggta gaggagctgt cccgccggac
     7441 gacgccgtag ccgcaacgcg acagacccgg atcgatgccg agcacgaaca ctcgttcgag
     7501 actagcggac cgaggcgggc gaggggtgga tcgcaggccg gcccgggtgc tctaggcctg
     7561 cgtccccgtc ctcacgtgtc gctggccgta tctgcgtcaa ggcgctcgac cggctgtccc
     7621 gtgacctgag cgatcagctc gaagtccttg tccaggtgca gaacggtcag acgggacagc
     7681 tcagccgtgg cagcaagcag gaggtcgggc aacgaggggc cacggtgctc gccccgctcg
     7741 gcaagggcag tgatcaccgc cagggcgcgg tcttcgattg ccggagtcag gtattcgatc
     7801 ggcatcgacg cgatcggggc accggtgagc ccggcgcgca tgtcgggcgc cgaccgggcg
     7861 gagtacgcca cctcgagcaa tgtgatggtc gtgatccgca ccagaccgcg ttcgatgcgt
     7921 tcggcccact cctcggcctc gggcgagctg cccagccgga ccagcgcaga cttgtcgatg
     7981 agccatggac cgggctgcat ctaggggcgc cgcgcccgat tcaggaccat gctcgagcca
     8041 tgacctcccg atcggccagg tccgcgaaga gcgtggcgaa acgtcgcagg tccgtgtccg
     8101 cgacggaaac ggcgcgatga cgagcgtcct ggtgcaaccg gcggcgcaga tattcgtttc
     8161 tcgacagacc gagcgcggcc gcgtgcgcgt ccaacgccgc aacatcggcg tcagggacat
     8221 cacggatcag gatgtcggac atggccccat cacctcctgt tgatatcaca tgatatcagg
     8281 tgggcgctac gtctccaccc cgtccaggat ctcgtcgggg atgtcgaagt tggcgaagac
     8341 gttctggacg tcgtcgaggt cctcgagcgc gtcgaccagc cggaggacct tgcgggcgtc
     8401 gccttcgttg tcgagcgcca cggtggtcgt gggcagcatg gtgaggtcgg ccgagtcgaa
     8461 ggcgatgccg gcggcttcga gggcgccccg cagggggttg agctcggcag gcggcgaggt
     8521 cagctgccag gtgtcgccct ggtcggagag gtcctcggcg ccggcctcga gggccacgag
     8581 catgaggtcg tcctcgctga cgtccttcgg caggatgatg gcgcccttgc gctcgaactg
     8641 ccaggacacc gcgcccggct cggccatcga cccgccgctg cgcgtgagca gcgaccgcac
     8701 gtctgcgccg gtccggttgc ggttgtcggt cagggtctcc acgatgagcg ccaccccggc
     8761 cggggcgtag ccctcgtagg agatcgactc gtaccggacc ccctccagct cgcccgtgcc
     8821 ccgcttgatg gcccggtcga tggtgtccgt cggcaccgag gcgtcgcgcg ccttctggaa
     8881 catgctgcgc agggtggcgt tcgaggtcag gtcgcccccg ccctcgcgcg ccgccacctc
     8941 tatctggcgg atcagcttgg cgaacagctt gccccgggcc ttgtcctgcg cccctttctt
     9001 gtgcttgatg gtggcccact tggaatgacc ggacatccgt cacacccctc ggaggaacag
     9061 ctcgtgcagc cggcagtcgc cggacagctc tggatggaag gccgccacca gcacccgccc
     9121 ctggcgacac agcaccggct ggcgtacgcc gtcggggcgc gccaccgtgg ccagcacgtc
     9181 gacggcgggc ccggcccgct cgacgacggg cgcccggatg aagacggccg gcaccgggcc
     9241 gccggcgacg ccgtccacgg gcagatccgt ctcgaaggaa tccacctggc gcccgaaggc
     9301 gttgcgccgc accaccaggt cgaccgcgcc taagcagcgc tggtcgtccc ggccgtcgac
     9361 cacggtcgag gccagcagga tcatcccggc gcacgtgccg aaggcggcca ggccgtcccc
     9421 cagccgctcg gccagcgcgt cgaacagtcc ggacgactcc agaagcatcg acatcgtcgt
     9481 cgactccccc cccggcagca cgagggcgtc gacgccgccc agctcgcccg gggacgtgac
     9541 ctcgacggtg tcgacgccca ggtcagcgag gacccgggcg tgctcccgga cgtcgccctg
     9601 gagggcgagg atgcctactt tcacctggcg gctcgggctc ggcccgcccg cgcgctcggc
     9661 ctctcccgcg ggcgatggtc accagccccg ctccgacaac cgcacgtcca cctggtcgat
     9721 ctcgatgccc accatgggct cgcccagccc cttgctcacc ttggccagga tggcggggtc
     9781 gcggtagtgg gtggtggcct ccacgatggc ccgggcccgg cgggccgggt cgccggactt
     9841 gaagatgccg gacccgacga acaccgcctg ggcgcccagc tgcatcacga gggcggcatc
     9901 ggccggggtg gcgatcccgc cggcgcagaa cagcggcacc ggcagcccgg ccgtctcggc
     9961 gatctcggcc accaggccga ccggcgcctg gagcgccttg gcccaggcgt agagctcggc
    10021 cccgtcggcc tgggtcacct tgcggatgtc gcccaggatg gcgcgcaggt ggcgcacggc
    10081 ctcgacgatg ttgccggtcc cggcctcacc cttggaccgg atcatggccg cgccctccga
    10141 gatccgccgc agcgcctcgc ccaggttggt cgccccgcac acgaacggca ccgcgaaggg
    10201 ccatttgtcg atgtgatggg cctcgtcggc gggggtgagg acctcgctct cgtcgatgta
    10261 gtcgacgccc agggcctgga ggacctgggc ctcggcgaaa tggccgatcc gcgccttggc
    10321 cattacgggg acggtcaccg tgtccttgat gccttcgatc agggccgggt cgctcatgcg
    10381 ggccaccccg ccgtcgcgtc ggatgtcggc cggcacccgc tcgagagcca tcacggccac
    10441 ggccccggcg tcctcggcga tcttggcgtg ctcgggggtg acgacgtcca tgatcacccc
    10501 gccgcggagc atctcggcca ggccgcgctt cacccgctcg gtaccggtgc gccgggcggg
    10561 accgtcggtg gtgcgggcgg gatcggtcat cacgcccagt ctaccggggg gacctcggcc
    10621 cgccccaaca ctttgccccg cgccccgccc tacagctcgc gcagcgtctc tatgcgctgc
    10681 gccagcggcg ccggaccgac cgcggcgggc tccccgacgc gcgcctccgg cggccgcagc
    10741 cacagatggg cgtcgcgggc cggcaccccg gcgggcccgg ccggcccgac ctgcaccttc
    10801 tcgagcgcgg cgagcaggcc cggggggtag cgggtcaggg ccacgcccgc caggtccgcc
    10861 gccacctccc gccgcgaggc ccgcgacggg ggcgaggacc gccatgcgcc ggccgcagcc
    10921 agcgcagccc ggcctccggg catggtcccc acgacg
//
LOCUS       DASWGG010000145         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3047957, whole genome shotgun sequence.
ACCESSION   DASWGG010000145 DASWGG010000000
VERSION     DASWGG010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3047957"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="VF954_02600"
     CDS             <1..1253
                     /locus_tag="VF954_02600"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530000.1"
                     /translation="GVLAYLVDAFRERGVDDVFLVPVSIAYDQLSEVAAMAAEEHGAE
                     KKAESFGWMLRYLRSQGQHLGKAYVNIGEPISLQRVRGEQKLATEKIAFEVLHRINRV
                     TPVTPTSLLTLALLGLGDRALTVAEIRSVLDPLLDYVAVRKLPVAGTWGPHQPPPGPA
                     TSPGGLHPALDALVASKVATRYAGGAEPVYAIGENQHLVAAYSRNRIIHFFVTRAITE
                     LVLLHLVRRPMGHPGEGDLGARAWAEALRLRDLLKYEFFFANKAEFAEDLRAELAILD
                     PDWERKSGDPTAIATGLAELPLLLGHRVLGSFLEAYMVVADRLAARPPGPVDEAAFLD
                     ECIGVGRQYRLQQRIHSTESISKELFRNALALAAGRGLLEGDDPAGRGAFAAELGEVV
                     DRVDSLRRLAVATLERTTAEEGPP"
     gene            1250..>1700
                     /locus_tag="VF954_02605"
     CDS             1250..>1700
                     /locus_tag="VF954_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEX9530001.1"
                     /translation="MTSRNDELQALLAEIEAGPEGPEIGAFFDFDGTLIAGYSAEAFV
                     LDAIRRRKVDPQTMIRSLLAGLDMQLRGSDVTALMEIAAEAVKGRREEALIELGQRLF
                     RERVAGTVYPEARAVVKAHQRKGHTLALASSATRFQAGPLAADLGIDN"
BASE COUNT          234 a          673 c          564 g          229 t
ORIGIN      
        1 tcggcgtgct cgcctacctc gtcgacgcct tccgggagcg gggcgtggac gacgtcttcc
       61 tcgtgccggt gtcgatcgcc tacgaccagc tgtccgaggt ggcggccatg gccgccgagg
      121 aacacggcgc cgagaagaag gccgagagct tcggctggat gctgcgctac ctccgctccc
      181 agggccagca cctcggcaag gcctacgtca acatcgggga gccgatctcc ctccagcggg
      241 tgcggggcga gcagaagctg gcgactgaga agatcgcctt cgaggtcctc caccgcatca
      301 accgggtcac cccggtcacg ccgacgtcgc tcctcacgct ggccctgctg ggcctgggcg
      361 accgggccct caccgtggcc gagatccggt cggtgctcga cccgctgctc gactacgtgg
      421 ccgtccgcaa gctgcccgtg gccggcactt gggggcctca ccagcctccc ccggggcccg
      481 caaccagccc cggcggcctg caccccgccc tcgacgccct cgtggccagc aaggtcgcca
      541 cccgctacgc cggcggcgcc gaaccggtct acgccatcgg cgagaaccag cacctggtgg
      601 cggcctactc ccgcaaccgc atcatccact tcttcgtcac ccgggccatc accgaactgg
      661 tgctcctcca cctcgtccgc cggcccatgg gccatcccgg ggagggcgac ctcggggccc
      721 gggcctgggc cgaggcgctg cggctgcgcg acctcctcaa gtacgagttc ttcttcgcca
      781 acaaggccga gttcgccgag gacctccggg ccgagctggc catcctcgac cccgactggg
      841 aacgcaagtc cggcgatccc accgccatcg ccaccggcct ggccgagctc ccgctgctgc
      901 tcgggcaccg ggtcctcggg tccttcctcg aggcctacat ggtggtggcc gaccgcctcg
      961 ccgcccgccc cccggggccg gtcgacgagg cggccttcct cgacgagtgc atcggcgtcg
     1021 gccgccagta ccggctccag cagcgcatcc actccaccga gtcgatctcc aaggagctgt
     1081 tccgcaacgc cctcgccctg gccgccggcc ggggcctgct ggagggcgac gacccagccg
     1141 ggcgcggcgc cttcgccgcc gagctggggg aggtggtcga ccgcgtcgac agcctgcggc
     1201 gcctggccgt cgccaccctg gagcgcacca ccgccgagga gggcccgcca tgacgagccg
     1261 caacgacgaa ctgcaggcgc tgctggccga gatcgaggcc ggccccgaag gccccgagat
     1321 cggcgccttc ttcgacttcg acggcacgct catcgccggc tactccgccg aggcgttcgt
     1381 gctcgacgcc atccgccggc gcaaggtcga cccccagacc atgatccgct ccctcctggc
     1441 cggcctcgac atgcaactgc ggggcagcga cgtcaccgcc ctcatggaga tcgccgccga
     1501 ggccgtgaag ggccggcggg aggaggcgct catcgagctg ggccagcgcc tcttccggga
     1561 gcgggtggcc ggcaccgtct accccgaggc ccgggcggtg gtgaaggccc accagcggaa
     1621 gggccacacc ctggccctgg cctcgtcggc gacgcgtttc caggccggtc cgctggccgc
     1681 cgatctgggc atcgacaacg
//
LOCUS       DASWGG010000146         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2723209, whole genome shotgun sequence.
ACCESSION   DASWGG010000146 DASWGG010000000
VERSION     DASWGG010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2723209"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /gene="glgP"
                     /locus_tag="VF954_02610"
     CDS             <1..1270
                     /gene="glgP"
                     /locus_tag="VF954_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX9530002.1"
                     /translation="HVLPNWSEADQAAWDRIDTARDDEVWRAREQGRDRLVAFVRTHL
                     RAAHLARGTSPSDVAWCDDVFDERVLTVGFARRFAGYKRATLLMSDPARLKALLLSAD
                     RPVQLAFAGKAHPADDVGKEMIRQVVQFARDPEIRHRFVFIEDYDIAVARTMYQGCDV
                     WLNTPRRPQEACGTSGEKAALNGALNCSILDGWWDEMFDGDNGWAISSAETYEDLERR
                     DQVEAASLFDLLEHQVVPLFYERNEGPVPRRWVGRVKSSLRSLGPRVTASRMVRDYVE
                     RMYEPVAGRADALAAADHREARELAAWKAKVIGAWRELKVAGVDSQTNVTDLGASREV
                     EALVSLGSLSGSDVEVQLLHGPVGPNDELAGGSVVTLALQGPGQEDGQYRYAGRFECN
                     QAGRYGFTLRVVAAHRHLAGFAEVGLVTWA"
     gene            complement(1286..>2074)
                     /locus_tag="VF954_02615"
     CDS             complement(1286..>2074)
                     /locus_tag="VF954_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9530003.1"
                     /translation="MAPGTPEPLVSLDRRDDGVAVVRLDRPKMNALCRDLLRQLQELA
                     EELAATPPGAVVVWGGERIFAAGADISEFGGPAEAREITALFHAALDAVGAIPRPTIA
                     AVTGYALGGGCELALACDLRIAAEDAKLGQPEILLGIIPGGGGTQRLARLVGPSRAKD
                     MIFTGRQVPADEALRIGLVDRVVPKGSAFGAALTLAGQLAAGPRQAFGLAKRAVDAGI
                     DRDLAGGLALERELFEDVFGTEDARIGVESFLADGPGRATFTGR"
BASE COUNT          290 a          754 c          758 g          272 t
ORIGIN      
        1 ccacgtgctg cccaactgga gcgaggccga ccaggcggcg tgggaccgca tcgacacggc
       61 gcgcgacgac gaggtgtggc gggcccggga gcagggccgg gaccgcctgg tggccttcgt
      121 ccgcacccac ttgcgcgccg cccatctggc gcgaggcaca tcgccttccg acgtggcctg
      181 gtgcgacgac gtgttcgacg agcgcgtgct gaccgtcggg ttcgcccgtc ggttcgccgg
      241 ctacaagcgc gccacgctcc tgatgtccga cccggcccgg ttgaaggccc tgctgttgtc
      301 cgccgatcgg cccgtgcagc tcgcgttcgc gggcaaggcc cacccggccg acgacgtcgg
      361 caaggagatg atccgccagg tggtccagtt cgcccgggac cccgagatac ggcaccgctt
      421 cgtcttcatc gaggactacg acatcgccgt ggcccggacc atgtaccagg gctgcgacgt
      481 gtggctcaac accccgcgcc ggccgcagga ggcgtgcggc accagcggcg agaaggccgc
      541 gctcaacggc gccctcaact gctcgatcct cgacggttgg tgggacgaga tgttcgacgg
      601 cgacaacggc tgggccatct cctcggcgga gacctacgag gacctggagc ggcgggacca
      661 ggtcgaggcg gccagcttgt tcgacctgct cgagcatcag gtcgtcccgc tcttctacga
      721 acgcaacgag ggcccggtcc cgcggcgctg ggtggggcgg gtgaagtcgt cgctgcgctc
      781 gttgggcccc cgggtgaccg cgtcgcgcat ggtccgcgac tacgtagagc gcatgtacga
      841 gcccgtcgcc ggccgggccg acgcgctggc cgcggccgac caccgggagg cccgcgagct
      901 ggccgcgtgg aaggcgaagg tgatcggcgc ctggcgcgag ctcaaggtgg ccggagtgga
      961 ctcgcagacc aacgtcaccg acctgggggc cagccgcgag gtggaggccc tggtcagcct
     1021 cgggtcgctg tccggcagcg acgtggaggt gcagctgctg cacggaccgg tcggccccaa
     1081 cgacgagctg gcggggggct cggtcgtcac gctggccctc cagggccccg gccaggagga
     1141 cggccagtac cgctacgccg gacgattcga gtgcaaccag gccggtcgct acggcttcac
     1201 cttgcgggtc gtcgccgccc accggcacct ggcgggcttc gccgaggtcg gcctggtcac
     1261 ctgggcgtga gctggggcgg cccggctacc tgccggtgaa ggtcgcccgg cccggcccgt
     1321 cggccaggaa ggactccacc ccgatccggg cgtcctcggt cccgaacacg tcctcgaaca
     1381 gctctcgctc gagcgccaga cccccggcca ggtctcggtc gatccccgcg tcgacagccc
     1441 gtttggccag cccgaaggcc tgacgggggc cggcggccaa ctggccggcc aaggtgagcg
     1501 cggcgccgaa ggccgatcct ttgggcacca cccgatcgac caggccgatg cgcagggcct
     1561 cgtcggccgg cacctggcgg cccgtgaaga tcatgtcctt ggcccgggac gggcccacca
     1621 accgggccaa ccgttgggtg ccgcccccgc cggggatgat gcccagcagg atctcgggct
     1681 ggccgagctt ggcgtcctcc gcggcgatgc gcaggtcgca ggcgagggcc agctcacacc
     1741 ctccccccag ggcatagccg gtcaccgccg cgatcgtcgg acgggggatg gcccccaccg
     1801 cgtcgagggc ggcgtggaaa agggccgtga tctcccgggc ctcggccggc ccgccgaact
     1861 ccgagatgtc cgccccggcc gcgaagatcc gctcccctcc ccagacgacg accgcacccg
     1921 gcggggtggc cgccagctcc tcggccagct cctggagctg gcgcagcagg tcgcgacaca
     1981 aggcgttcat cttgggccga tcgaggcgca cgacagcgac tccgtcgtcc cggcggtcga
     2041 gggagaccag gggttcgggg gtgcccgggg ccat
//
LOCUS       DASWGG010000147         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_392045, whole genome shotgun sequence.
ACCESSION   DASWGG010000147 DASWGG010000000
VERSION     DASWGG010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392045"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /gene="menC"
                     /locus_tag="VF954_02620"
     CDS             <1..1017
                     /gene="menC"
                     /locus_tag="VF954_02620"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEX9530004.1"
                     /translation="ILLVRAIGFGSADGWAECAALAAPSYTSEYVEGAHRVIREHLAP
                     LVLGAGDLAVDTVAGLLAPVRGHPMAKAAVETAILDAELRAAGVSLSAYLGGVRTEVD
                     AGVSLGIAGSLPELLAAAAAHVELGYRRVKLKIQPGWDLEPVRAVRDHLGDQLMLQVD
                     ANGAYAVADAGHLALLDPYGLALIEQPLAPDDLLGHAELGRRIATPICLDESITSARD
                     AATAITLGACSIVNVKPGRVGGYLEARRVHDVCVAHGVAAWCGGMLETGIGRAANLAL
                     AALPGFTLPGDLSASDRYYRRDITEPFRLHGGRLRVPDGPGIGVVPEPAALKACTTSV
                     EVIR"
     gene            complement(1088..1351)
                     /locus_tag="VF954_02625"
     CDS             complement(1088..1351)
                     /locus_tag="VF954_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530005.1"
                     /translation="MTQREVLEAAVRAQAAERLPWERAEHCVFESALRERLAQVGITP
                     DADLAVALMATAMLLAEASPEWGGDYRDALADVAQAGLALLPE"
     gene            complement(1357..>2134)
                     /locus_tag="VF954_02630"
     CDS             complement(1357..>2134)
                     /locus_tag="VF954_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX9530006.1"
                     /translation="APTTYDKVLALQDWMAANTHYTLNIPDLPGRADAVDQYLFVDRR
                     GFCEQIASSLVVMLRSLGVPARVVAGYAPGQRNPLTGLYEVRASDAHAWAEVWFPGAG
                     WQSFDPTAEVPLAGDAQAGRAAQGLGTYLAARFPHVPKSVGFPVGAAAGLVALLWVGR
                     RPLAAFRSRRRPVSWEERCQRRVEQWGAAQGRPRQPAETLPEYVGVLGAGPPLARAAA
                     IVSRAAFGPVLPSEAEREEVEAALATGPLAEVYAGGGYRG"
BASE COUNT          249 a          840 c          789 g          256 t
ORIGIN      
        1 atcctcctgg tgcgggcgat cggcttcggc tccgccgatg gctgggccga gtgcgccgcc
       61 ctggcggcgc cgtcctacac gtcggagtac gtcgaggggg cccaccgggt catccgtgag
      121 cacctcgccc ccctcgtgct gggagccggc gatctcgccg tggacacggt cgccggtctg
      181 ctggcgccgg tcaggggcca ccccatggcc aaggccgccg tcgagacggc gatcctggac
      241 gccgagctgc gcgccgccgg cgtctcgctg agcgcctacc tcggcggcgt ccggaccgag
      301 gtcgacgcgg gcgtctccct cgggatcgcc gggtcgctgc cggagctgct cgccgccgcc
      361 gccgcccacg tcgagctcgg ctaccgccgg gtcaagctca agatccagcc cggctgggac
      421 ctggagccgg tgcgggcggt gcgggaccac ctcggcgacc agctcatgct ccaggtggac
      481 gccaacggcg cctacgcggt ggccgacgcc gggcacctgg cgctcctcga cccctatggg
      541 ctcgccctca tcgagcagcc cctcgccccc gacgacctcc tcggccacgc cgagctgggc
      601 cggcggatcg ccaccccgat ctgcctagac gagtccatca cctcggcccg cgacgcggcc
      661 accgccatca cgctgggcgc ctgctcgatc gtcaacgtca agccgggccg ggtgggcggc
      721 tacctcgagg cccgccgggt ccacgacgtg tgcgtggccc acggcgtggc ggcctggtgc
      781 ggcgggatgc tcgagacggg gatcgggcgg gccgccaacc tggcgctcgc cgccctcccc
      841 ggcttcaccc tcccgggcga cctgtcggcc agcgaccgct actaccgccg cgacatcacc
      901 gagccgttca ggctgcacgg cggccgcctg cgcgtgccgg acggcccggg catcggcgtg
      961 gtgcccgagc ctgcggccct gaaggcgtgc accacgtcgg tcgaggtcat ccggtgagcc
     1021 gggaggccgg cgtcgggccg gcgcctcgcc tcgccgggcg cccggtcgct gccagtttcg
     1081 gtgcgggcta ttccgggagg agcgccagcc cggcctgggc gacgtcggcc agggcgtcgc
     1141 ggtagtcgcc gccccactcg ggactggcct cggccagcag catggccgtg gccatgaggg
     1201 cgacggccag atcggcgtcg ggcgtgatgc ccacctgggc caaccgctcc cgcagcgcgg
     1261 actcgaacac gcagtgctcg gcccgctccc agggcagccg ctcggcggcc tgcgcccgca
     1321 ccgccgcctc cagcacctcc cgctgcgtca cctgcctcac ccccggtagc caccaccagc
     1381 gtagacctcg gcgagcggtc cggtcgcgag cgccgcctcc acctcctccc gctccgcctc
     1441 ggagggcagg acgggcccga aggcggcccg gctcacgatg gccgcggcgc gcgccagcgg
     1501 cgggccggcg ccgagcacgc cgacgtactc gggcagcgtc tcggccggct gccgcggcct
     1561 cccctgggcg gcaccccact gctccacgcg gcgctggcac ctttcctccc agctcaccgg
     1621 ccggcggcgg gaccggaacg cggcgagcgg ccggcggccc acccacagga gcgccaccag
     1681 cccggccgcg gcgcccaccg ggaagccgac cgacttcggc acgtgcggga accgggcggc
     1741 caggtaggtc cccagtccct gggcggcccg gccggcctgg gcgtcgccgg cgaggggcac
     1801 ctcggctgtc gggtcgaagg actgccagcc ggcgccgggg aaccacacct cggcccaggc
     1861 gtgggcgtcc gacgcccgga cctcgtagag cccggtgagg gggttgcgct gcccgggggc
     1921 gtagccggcc acgacccggg ccggcacgcc gagggaccgc agcatgacga ccaggctgga
     1981 ggcgatctgc tcgcagaacc cccgccggtc gacgaacagg tactggtcca cggcgtcggc
     2041 ccggccgggc aggtccggga tgttcagggt gtagtgggtg ttggcggcca tccagtcctg
     2101 gagggcgagg accttgtcgt aggtggtggg ggcg
//
LOCUS       DASWGG010000148         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1432352, whole genome shotgun sequence.
ACCESSION   DASWGG010000148 DASWGG010000000
VERSION     DASWGG010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432352"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="VF954_02635"
     CDS             <1..1495
                     /locus_tag="VF954_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX9530007.1"
                     /translation="LSRVHRAVWGLHFAGFLTFLVLLPTTKLRHMFTSPMNMYLRARP
                     RPKGAMKPLPNLMETELDSFGASRIEDFTWKQLMDTDACTVCGRCTSVCPAHATGKPL
                     DPREIVLKVGHVMAATASPKVAPPVTVDEEITISAGSVLERITAEEVWACTTCKACDE
                     ICPVNIEILDKILDVRRYYSLMESNFPTELGNAYRGMENSSNPWGLSQGDRAQWAKGL
                     PDEVPIVDPSSGSLDHEYLYWVGCATSFDDRNKKVAVATAKLLQRAGVDFAILGQNEL
                     CTGDPARRSGNEYVFQMLALQNIDALNGLGVQKIIASCPHCFNTLKNEYPDYGGRFEV
                     IHHSQFLSQLVEDGRLSMAGASLGERIAYHDSCYLGRHNDVYLAPRKVIGSIAGVEVV
                     EMPRNGTRGLCCGAGGARMFMEERTGKKVNIERTEEALGTGADRIAVACPFCMIMLDD
                     GVKEKGADEQVKVSDISMVLLEAIEGIPTRAASAAMAPGVTPGGPAS"
     gene            complement(1492..>2080)
                     /locus_tag="VF954_02640"
     CDS             complement(1492..>2080)
                     /locus_tag="VF954_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9530008.1"
                     /translation="DAMVELITVVEGFFACGVAASVYGVDDGAGGVMPDPVFANIGKL
                     LLATKIYDMHRLAHYVSGGLVVALPGPDEDHNPETAASLAAVLGGRADIPAAARLEVA
                     RLVEDLTASYQGGWYSVISLHGGGSPEAMKREIWRNYPVGDKVDLVERLLDRGVAGDA
                     AGGGTAGLPSIRRRVSRQPGRCCDAGCDAREGGAG"
BASE COUNT          327 a          737 c          732 g          284 t
ORIGIN      
        1 gctgtcccgg gtgcaccggg cggtgtgggg gctgcacttc gccggtttcc tcaccttcct
       61 ggtgctgctg ccgacgacca agctgcgcca catgttcacg tcgccgatga acatgtacct
      121 gcgggcccgg ccccgcccca agggcgccat gaagccgctg cccaacctga tggagaccga
      181 actcgactcc ttcggggcgt cgcggatcga ggacttcacc tggaagcagc tgatggacac
      241 cgacgcctgc accgtgtgcg gccggtgcac ctccgtgtgc ccggcgcacg cgaccggcaa
      301 gccgctcgac ccgcgcgaga tcgtgctcaa ggtcggccac gtcatggccg ccaccgcttc
      361 cccgaaggtg gcgccgccgg tcaccgtcga cgaggagatc acgatctcgg ccgggtcggt
      421 gctggagcgg atcaccgccg aggaggtgtg ggcctgcacc acctgcaagg cctgcgacga
      481 gatctgcccg gtcaacatcg agatcctcga caagatcctc gacgtccgcc gctactactc
      541 gctgatggag tcgaacttcc ccaccgagct cggcaacgcc taccggggga tggagaactc
      601 gtcgaacccc tggggcctca gccagggcga ccgggcccag tgggccaagg gcctgcccga
      661 cgaggtgccg atcgtcgacc cctcctccgg gagcctcgac cacgagtacc tctactgggt
      721 cggctgcgcc accagcttcg acgaccgcaa caagaaggtg gcggtggcca cggccaagct
      781 gctgcagcgg gccggggtcg acttcgccat cctgggccag aacgagctct gcaccggcga
      841 ccccgcccgg cgctcgggca acgagtacgt gttccagatg ctggcgctgc agaacatcga
      901 cgccctgaac gggctcgggg tgcagaagat catcgccagc tgcccgcact gcttcaacac
      961 gctcaagaac gagtaccccg actacggcgg ccgcttcgag gtcatccacc actcgcagtt
     1021 cctcagccag ctggtggagg acgggcgcct gtcgatggcc ggggcgtcgc tcggcgagcg
     1081 catcgcctac cacgactcct gctacctcgg ccgccacaac gacgtgtacc tcgccccccg
     1141 caaggtgatc ggatcgatcg ccggcgtgga ggtggtggag atgccccgca acggcacccg
     1201 gggcctgtgc tgcggcgccg gcggggcccg catgttcatg gaggagcgga cgggcaagaa
     1261 ggtcaacatc gagcgcaccg aggaggcgct gggcaccggc gccgaccgga tcgccgtggc
     1321 ctgcccgttc tgcatgatca tgctggacga cggcgtgaag gagaagggcg ccgacgagca
     1381 ggtcaaggtc agcgacatct cgatggtgct gctcgaggcc atcgagggga tcccgacccg
     1441 ggccgcctcg gcggcgatgg cgcccggcgt gacgccgggc gggccggctt cttagccggc
     1501 cccgccctcc cgggcgtcgc agccggcgtc gcagcagcgg cccggctggc gggagacccg
     1561 gcggcggatg gacgggaggc cggcggtccc gccgccggcg gcgtcgccgg ccacgccccg
     1621 gtcgaggagc cgctcgacga ggtcgacctt gtccccgacg ggatagttcc gccagatctc
     1681 ccgcttcatc gcctcggggg agccgccgcc gtggagcgag atcaccgagt accagccgcc
     1741 ctggtaggag gccgtcaggt cctccaccag gcgggcgacc tcgaggcggg cggcggcggg
     1801 gatgtcggcc cgtccgccga gcaccgccgc cagggaggcg gccgtctcgg ggttatggtc
     1861 ctcgtcgggg ccgggcaggg ccacgaccag gccgccggag acgtagtggg ccaggcggtg
     1921 catgtcgtag atcttcgtgg ccaggaggag cttgccgatg ttggcgaaga ccgggtcggg
     1981 catgacgcca ccggcgccat cgtcgactcc gtagaccgac gccgccaccc cgcaggcgaa
     2041 gaagccctcg accacggtga tgagctccac catggcgtcg
//
LOCUS       DASWGG010000149         3983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_70543, whole genome shotgun sequence.
ACCESSION   DASWGG010000149 DASWGG010000000
VERSION     DASWGG010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70543"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VF954_02645"
     CDS             complement(<1..839)
                     /locus_tag="VF954_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530009.1"
                     /translation="MASAGSRAAAARRLDPDWVRPHSWEQGDGGRAERTVIGNLGSGR
                     HATVDGRGLVTAWQDGWSLDWWVRAEDRWHLPSRQAAVRQRLVEATPVVETAMRIPGG
                     DAVERAYAVAPPMGPGRAAGELVVVEVDNQSPVPVAVAFALRPYHPDGLAVVHRVTLE
                     DRTVLVDGLPALFLPRPPAQMAASTFGAGDVAGPVLAGEAGHGWPNGLRCRVGLATAA
                     FVFPVPHRTTIRVAVPLSAERRARRRVSAPLDVASLPASDAVVRGWVAQTRRGMSVVL
                     PPGR"
     gene            complement(823..1830)
                     /locus_tag="VF954_02650"
     CDS             complement(823..1830)
                     /locus_tag="VF954_02650"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX9530010.1"
                     /translation="MPTKVAVVGAGSWGTAVAAIAATRAPAVVWARRAELAREIGEHH
                     ANDSYLPGVRLPAALEATDSLEAALGGAGVVVMAVPSHGFRDIFTRARPWLPVGAPVL
                     SLTKGIEQGTLKRMTQIVSELSPTTPAGVLTGPNLAQEVTAGYPAASVVAFADAVVAR
                     DVQQLFSTGAFRVYTNPDVAGCEIAGALKNVMAIAAGIADGLGLGDNTKAALITRGLA
                     ELTRLGVALGGEPLTFSGLAGMGDLVATCTSRQSRNRHVGEQLGRGKSMDEIVEEMSM
                     VAEGIKTSLVVVELASRVGVEMPIAEQVVAVLYDGKKPADVISSLMQRQAKPELHGIS
                     G"
     gene            1855..2466
                     /gene="cofC"
                     /locus_tag="VF954_02655"
     CDS             1855..2466
                     /gene="cofC"
                     /locus_tag="VF954_02655"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX9530011.1"
                     /translation="MGRDGRIAAVGATVGVLLPVKSFAVAKARLAAVLDGPGREALAR
                     SLAERTLRAAHELPVTVVCDDRDVARWARSRGAAVVADPGGGLSSAVRAGVAYMEGAG
                     LGSVIVAHADLPWAEDLRPVAGFDGVTLVPDRHEDGTNVICVPAGCGFDFSYGPGSFA
                     RHRAAATRTGRPVRIMRDAALAWDVDEPADLPAGEAFGAVRHL"
     gene            complement(2527..2892)
                     /locus_tag="VF954_02660"
     CDS             complement(2527..2892)
                     /locus_tag="VF954_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530012.1"
                     /translation="MAIAKKAAPAAKKTAVKKAAPAAKKAPAKKTTAAKKAPAKKTTA
                     AKAPAKRTTVRKTTAAKKAPARKAPAKRTAVRKTAAAKKAPARRAPTKKATTARKTAA
                     AKKAPARKAPARKAPAAKK"
     gene            complement(3032..3298)
                     /locus_tag="VF954_02665"
                     /pseudo
     CDS             complement(3032..3298)
                     /locus_tag="VF954_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009794840.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     regulatory      3518..3624
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3614..>3983
                     /locus_tag="VF954_02670"
     CDS             3614..>3983
                     /locus_tag="VF954_02670"
                     /inference="COORDINATES: protein motif:HMM:NF025048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530013.1"
                     /translation="MSNSVSRTNVSRAKVYVTGSRRDVRVPFTEVSLSDSPGRSGPQP
                     NEPVRLYDTSGPGSDPAQGLPALRRDWIARRGDTEEYDGRPVRPRDDGRGALRRAGIT
                     SSPDQGRPRRAQPGRTVTQMH"
BASE COUNT          509 a         1429 c         1425 g          620 t
ORIGIN      
        1 cgcccgggtg gcagcacgac gctcatgccc cgccgggtct gcgccaccca gccgcgaacc
       61 accgcatcgc tcgccggcag cgacgccacg tcgaggggcg cggacacccg ccggcgggcg
      121 cgccgctcgg cggacaacgg cacggccacg cggatggtgg tgcgatgtgg caccggaaag
      181 acaaaggcag cggtggccag ccccacccgg cagcgcagcc cgttaggcca cccgtggccg
      241 gcctccccgg ccagcaccgg cccggccacg tcgcccgcgc cgaacgtcga tgccgccatc
      301 tgcgccggag gccgcggcag gaacaacgcc ggcaggccgt cgaccaggac ggtgcgatcc
      361 tcgagggtga cgcggtggac gacggccagg ccgtcggggt ggtaggggcg cagcgcgaac
      421 gcgacggcga cgggcacggg cgactggttg tcgacctcca cgacgaccag ctcgccggcc
      481 gcgcggcccg gtcccatcgg gggggccacg gcgtaggccc gctcgaccgc gtcgcctccg
      541 gggatgcgca tggccgtctc caccaccggc gtggcctcga ccagccgctg ccgcaccgcc
      601 gcctgccggg acggcaggtg ccagcggtcc tcggctcgga cccaccagtc gagggaccac
      661 ccgtcctgcc acgcggtgac caggcctcga ccgtcgaccg tcgcgtgccg gcccgagccg
      721 aggttgccga tcaccgtgcg ctccgctcga ccgccgtccc cctgctccca cgagtggggc
      781 cggacccagt cggggtcgag ccggcgggcc gcggccgccc gactacccgc tgatgccatg
      841 caactctggc ttggcctgcc gttgcatgag cgagctgatg acgtcggccg gcttcttccc
      901 gtcgtaaagg acggccacga cctgctcggc gatgggcatc tcgacaccca cacgagaggc
      961 cagctcgacc acgacgagcg aggtcttgat cccttcggcg accatggaca tctcctccac
     1021 gatctcgtcc atggacttgc cccgaccgag ctgctcgccc acgtggcggt tgcggctctg
     1081 ccggctcgtg cacgtggcga ccagatcgcc catgccggcc aggccggaga aggtcagcgg
     1141 ctcgccaccg agcgcgaccc cgaggcgcgt caactcggcg agccccctcg tgatcagcgc
     1201 ggccttggtg ttgtcgccca ggcccaggcc gtcggcgatc ccggcggcga tggccatcac
     1261 gttcttgagc gccccggcaa tctcgcatcc ggccacgtcg gggttggtgt acacccggaa
     1321 cgcgccggtc gagaaaagct gctggacgtc ccgggccacg accgcatctg cgaaggccac
     1381 gacgctcgcg gccgggtacc ccgcggtcac ctcctgggcc aggttgggcc ccgtgagcac
     1441 gccggccggc gtggtcgggg acagctccga cacgatctgg gtcatgcgct tcaacgtgcc
     1501 ctgctcgatc cccttggtga ggctcaggac cggggcaccg acgggcaacc agggacgcgc
     1561 ccgggtgaag atgtcgcgaa agccgtgcga gggcaccgcc atgacgacca cgccggcgcc
     1621 gcccagagcc gcctcaaggc tgtcggtggc ctctagcgcc gccgggagcc tcacaccggg
     1681 caggtaggag tcgttggcgt ggtgctcgcc gatctcccga gcgagctcgg cgcgcctcgc
     1741 ccacaccacg gccggcgccc tcgtggcggc gatggccgcc accgcggtgc cccacgaccc
     1801 cgccccgact accgccacct tggttggcac ggcgcccacc ctagacttcc gcgtgtggga
     1861 cgagacgggc gcatcgccgc cgtcggtgcg accgtgggcg tgctgctgcc ggtgaagtcc
     1921 ttcgcggtcg ccaaggccag gttggcggcc gtgttggacg ggccggggcg cgaagccttg
     1981 gcccgctcgc tggcagagcg caccctgcgg gccgcccacg agctgcccgt caccgtcgta
     2041 tgtgacgacc gcgacgtcgc ccgctgggcc aggtcgcggg gtgccgccgt ggtcgcggac
     2101 ccgggcggcg ggctgagctc ggcggtccgc gccggggtgg cctacatgga gggcgccggc
     2161 ctgggctcgg tgatcgtggc ccacgccgac ctgccctggg ccgaggatct acgcccggtg
     2221 gcgggcttcg acggggtgac gctagtgccg gaccgccacg aggacggcac caacgtgatc
     2281 tgcgtgccgg cggggtgcgg gttcgacttc tcctacggcc ccggctcctt cgcccgtcac
     2341 cgggcggccg cgacccgaac cggccggccg gttcgcatca tgcgcgacgc ggcgctggca
     2401 tgggacgtcg acgagcccgc cgatctcccc gcgggcgagg cgttcggcgc cgtgcgccat
     2461 ctctagcaat gcgaaaaggg gaggggccgc cgaccacctc cctttccgtg ttgcttcctg
     2521 gcggcgctac ttcttggcgg ccggggcctt gcgggccggg gccttgcgcg ccggggcctt
     2581 cttggcggcg gccgtcttgc gggcggtggt cgccttcttg gtcggcgccc ggcgcgccgg
     2641 ggccttcttg gcggcggccg tcttgcgtac ggccgtcctc ttggccgggg ccttgcgggc
     2701 cggggccttc ttggcggcgg tcgtcttgcg gacggtcgtc ctcttggccg gggcctttgc
     2761 cgcggtggtc ttcttggccg gggccttctt ggccgcggtc gtcttcttgg ccggagcctt
     2821 cttggccgcg ggggcggcct tcttcaccgc agtcttcttg gccgccggag cggccttctt
     2881 tgcaattgcc atggctgtct ctccgctcct cgggttagcg cttcttggtc gacttggcgg
     2941 ccttggccgc cttcttggtg gtcgaagacg ccttcttggc cggtgctgcc ttcttggccg
     3001 gtgcggcctt cttcgctcct cccttggcat tcagggaagt cttgaacgcg gtcccggggg
     3061 cgaaacggac gcccttcgag gcggcgatct tcaccggtgc cccagtctgc gggttgcgcc
     3121 ctgtccgggc tgcgcgagca gtcgggttga acgagccgaa gccgaccagg ctgaccttgt
     3181 tgccggcttt caccgcgccc atgaccgtgc ggacaaatgc gtcgatggct ccttcggcct
     3241 gacgtttgtc gaggccggac gagccactca ccgcttcgat gagctctgat ttgttcaccg
     3301 ccacctcctg gcgtgttggg gaagtccctg aaatgaaaga ggtttcgcgc ctccaggtca
     3361 agcatttccc ttgagacaca agggtttcta ttttcaattc gactgaagac ccttgtgctg
     3421 caagggtttc gggtcccgga gaaacttttg ggcccgaggg cgtaatgggc gcgtctgacg
     3481 gcattggcgg tgctcggcgt cgatcgacgc gcgagactga gagctgcacg ggagcccgcg
     3541 tgcgcgggct gagagggcgt gaaggcgccg accgtagaac ctgatccggc taagaccggc
     3601 ggagggagcg aagatgagca acagcgtttc gaggaccaac gtgtcgagag ccaaggtcta
     3661 cgtcaccggc agccggcggg acgtccgggt gccgttcacc gaggtctcgt tgagcgactc
     3721 gcccgggcgc tcgggcccgc agccaaacga acccgtccgg ctctacgaca ccagcgggcc
     3781 aggctccgac ccggcgcagg gtttgccggc tcttcgccgt gattggatcg cgaggcgcgg
     3841 cgacaccgag gagtacgacg ggcgaccggt gcggccccgc gacgacggtc ggggcgcgtt
     3901 gcgccgtgct gggatcacct cttctcccga ccagggtcgg ccccggaggg cccagccggg
     3961 gcgcaccgtg acgcagatgc act
//
LOCUS       DASWGG010000150         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1121937, whole genome shotgun sequence.
ACCESSION   DASWGG010000150 DASWGG010000000
VERSION     DASWGG010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121937"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VF954_02675"
     CDS             <1..533
                     /locus_tag="VF954_02675"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX9530014.1"
                     /translation="GADPPSKPGADPPASPGPIVDGPVAVYPPGSAPLVTVSPARVLA
                     AHPERLALGPIFDRAAHRAHVPAQLLKAIAWQESGWQNNVFSVVNAQGIGQLMPGTVA
                     FLNALTGQHLDPAKPEDNITLEATLVAFLLYNTRGDQPTAVAGYYQGLASVQQRGLYD
                     DTKAYVANVLALRAKF"
     gene            995..1459
                     /locus_tag="VF954_02680"
     CDS             995..1459
                     /locus_tag="VF954_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HEX9530015.1"
                     /translation="MADDDELDEAEPDLDEAELEDPDLEEADLEDPEDEDLLEDEEVL
                     DVDLDDEVEADAEPAAVVAPAPDEDEEEDEDEDGEDEDVEASLDVILKERLVVPDEED
                     EDDEAPDVDDRSESSARVLPKQPDEFVCQSCFLVKHPSQLADAGRMLCRDCV"
     gene            1544..2068
                     /locus_tag="VF954_02685"
     CDS             1544..2068
                     /locus_tag="VF954_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530016.1"
                     /translation="MGDDKTPLDQLLDLLFYAPVGVALTATEELPRMVEKGRSRVGTQ
                     VSLARMIGRFALAEGQRQATAHLRQVSQRLAAPTRAQAGAASRPAPSGDAAGTPSPWG
                     NGHVPAPPAARRPDGAGLAIPGYDTLSASQVVQRLAGLAAEELDAVLSYESATRGRRT
                     VLNRIAQLQNDEDS"
     gene            2187..2567
                     /locus_tag="VF954_02690"
     CDS             2187..2567
                     /locus_tag="VF954_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9530017.1"
                     /translation="MFLARELGSGDIRGRLQAALTRPTAVVVAGGLGPLTVGVGVAHR
                     EELGDGSRLGRIDVLFVEPGGRGVGVGEAVMAGLLGWCASAGCAGVDATALPGNREAK
                     NFFEAAGFSARLLVMHHRMPGADD"
     gene            2578..>2800
                     /locus_tag="VF954_02695"
     CDS             2578..>2800
                     /locus_tag="VF954_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9530018.1"
                     /translation="MTGSVTGREDEPPAGRPQVCVGAICVDADRLLLVRRGRGPAAGE
                     WSVPGGRVEGGETLAEAVVRELAEETGLEG"
BASE COUNT          412 a         1014 c         1036 g          338 t
ORIGIN      
        1 cgggggccga tccgccctcc aagccgggag ccgatcctcc cgccagcccg gggccgatcg
       61 tcgacgggcc ggtggccgtc tacccgccgg ggtcggcgcc gctcgtcacc gtcagcccgg
      121 cccgcgtcct cgccgcccac ccggagcgcc tggcgctggg gccgatcttc gaccgggccg
      181 cccaccgggc ccacgtcccg gcccagctgc tcaaggccat cgcctggcag gagtccggct
      241 ggcagaacaa cgtcttctca gtggtgaacg cccagggcat cggccagctg atgcccggca
      301 cggtggcctt cctcaacgcc ctcaccggcc agcacctcga cccggccaag cccgaggaca
      361 acatcaccct cgaagccacc ctggtggcct tcctgctgta caacacccgc ggagatcagc
      421 ccaccgccgt cgccggctac taccagggcc tggcgtcggt ccagcagcgc gggctctacg
      481 acgacaccaa ggcgtacgtg gccaacgtgc tcgccctgcg cgccaagttc taggcgcgcc
      541 cagtcccagg gcgcgtcaag ctccagccgg gtcgaacggg tcgcagcccc cgcggcccga
      601 caggccgaga attcctgccg atcggccctt gactgggcgc tcccggcgca ccaagatcga
      661 tccagtccga acgcaccccc aagccaggtc gtgggcctcc cgcgggcagt tccgcccggg
      721 ggagggggca gcagagggca ccgggggtcc aggagaagcc gagtagagaa cgtcccaata
      781 acctgcccga gtcggcccca gaagcctgcg ggaaaggcta ccggacggcc ccaaaggctt
      841 cctggacctc ctccatcgag ggcgtggaat acctgtggtg cagcggcgtt gcacggttgc
      901 ccgtccgttg ggcgcggacc gcgccggcgc agctccggcg acggatatgc tccgccccct
      961 cgtatcccac ggacccgcca cccaaggacc attgatggcc gacgacgacg aactggacga
     1021 agccgagccc gacctggacg aggccgagct cgaggatcct gacctcgagg aggccgacct
     1081 cgaggacccc gaggacgagg acctgctcga ggacgaggag gtcctcgacg tggacctcga
     1141 cgacgaggtc gaggcggatg ccgagcccgc cgcggtcgtg gcgcccgcgc ccgacgagga
     1201 cgaggaggag gacgaggacg aggatggcga ggacgaggac gtggaggcca gcctcgacgt
     1261 catcctgaaa gagcggctgg tggtgcccga cgaggaggac gaggacgacg aggcgcccga
     1321 tgtcgacgac cgctcggaga gctcggcgcg ggtgctgccg aagcaacccg acgagttcgt
     1381 ctgccagtcg tgcttcctgg tgaaacatcc cagccagctg gcggacgcag ggcgcatgct
     1441 ctgccgcgat tgcgtgtgat ggagggtgtc gggccaggcc gctgggcgtc ggtggcgatc
     1501 ggcggcaaga atggagctct gaagtcgggg aaggacgaca aacatgggtg acgacaagac
     1561 gccgctggac cagctgctcg acctgctctt ctacgcgccg gtcggggtgg ccctcacggc
     1621 caccgaggag ctgccccgga tggtcgagaa gggccgcagc cgggtgggca cccaggtgtc
     1681 gctggcccgc atgatcgggc gcttcgcgct ggccgaaggc cagcgccagg ccactgccca
     1741 cctccgccag gtgagccagc ggctggcggc cccgacccgc gcccaggccg gcgccgcctc
     1801 ccgcccggcg ccgtccgggg acgccgccgg tacgccgtcg ccatggggca acggccacgt
     1861 gccagccccg cccgccgccc gccgcccgga cggcgccggg ctcgccatcc ccggctacga
     1921 cacgctctcc gcgtcgcagg tcgtgcagcg cctggccggc ctggccgccg aggagctcga
     1981 tgccgtcctg tcctatgaat ccgccacccg gggccggcgt accgtcctca accggatcgc
     2041 ccagctccag aacgacgaag actcctgagc tgtctacacc tgagctgaga gccgccgccc
     2101 gacccgcgac cacggacgac ctcgagcgcc tggccgagct gtgcgacacc gccgcccggg
     2161 aggccgccga ggcccggggc gggcccgtgt tcctcgcccg ggagctcggg agcggcgaca
     2221 tccggggccg gctccaggcg gcccttaccc gccccaccgc tgtcgttgtg gccggtgggc
     2281 tcggacccct cacggtcggc gtcggcgtcg cccaccgcga ggagctgggg gacggcagcc
     2341 ggttggggcg catcgatgtg ctcttcgtcg agccgggagg gcgcggggtg ggcgtgggcg
     2401 aagccgtcat ggcgggcctg ctgggctggt gcgcgtcggc gggttgcgca ggcgtggacg
     2461 ccaccgccct gcccggcaac cgggaggcca agaacttctt cgaggcggcg ggcttcagcg
     2521 cccggctgct ggtgatgcac caccggatgc cgggcgccga tgattgatgc gtgagccgtg
     2581 accgggtccg tgacggggcg cgaggacgag ccgccggcag ggcggccgca ggtgtgcgtc
     2641 ggggcgatct gcgtcgacgc cgaccgtctc ctcctggtgc ggcgcggccg gggcccggcg
     2701 gcgggggagt ggtcggtccc gggcgggagg gtcgaggggg gcgagaccct cgccgaggcc
     2761 gtggtgcgcg agctcgccga ggagacgggg ctggaggggg
//
LOCUS       DASWGG010000151         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1121970, whole genome shotgun sequence.
ACCESSION   DASWGG010000151 DASWGG010000000
VERSION     DASWGG010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121970"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VF954_02700"
     CDS             <1..223
                     /locus_tag="VF954_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9530019.1"
                     /translation="PLQQAMWERHSFQCGFCTPGFVMQATELLREDPEPTEGQVREAL
                     SGNICRCTGYESIVEGVLLAARRLAAESG"
     gene            279..>2180
                     /locus_tag="VF954_02705"
     CDS             279..>2180
                     /locus_tag="VF954_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9530020.1"
                     /translation="MAERFVGSSVRRVEDHRILTGRGRYVDDVVLPAMVHAAFVRSPF
                     PHARILGIDVAAARALAGVVAVYTGAEMEEMVVSGPGPLAMMVPGGRSPSFTLIATDK
                     VRLVGDLVALVVAESRAVAEDACELIEVDYDPLPAVASAEQALDPSRPAVFEDLGSNV
                     LAGPTLDTWGDVEGVFASSDRVLRTTIRQHRHQNVPMETRGSVASFDAGAGQLTVHAA
                     TQGVGMTQRGMSMQLGLPMEQIRVLAGDIGGSFGLKFGAGREDIAVAAASRALHRPVK
                     WIEDRNENLVASGQAREESFDVQAAFMDDGRVRGLKVKMVLDSGAYPGMGAMLGAMIA
                     RTIPSCYQLEALEFESTTVVTNKASYVAYRGPWAAETFVRERLLDLIARELGMEPIEV
                     RLRNVVTRGQAPLEMVTGQSLAGVTSRQSLERVAELVDVGAFRERQRGAAAEGRHLGL
                     GMATYIEAAPGPRAKGGRRGPTGNELMRMRLEPDGTVSVFTSQMPHGQGHQTTLAQVA
                     ADELGMPLEMVNVVVGDSDVVPAGFGTGGSRSAVMAGGNALHTARKIRQLVLERASQL
                     MEIDAEDLEIVGDAVAARGVPARALTLAEVAVAGDDRLAGVGGILEVENAYDGGEGGW
                     AGGTHCAIVE"
BASE COUNT          311 a          765 c          816 g          288 t
ORIGIN      
        1 ccccctccag caggccatgt gggagcgcca cagcttccag tgcgggttct gcaccccggg
       61 cttcgtgatg caggcgaccg agctgctgcg cgaggaccct gagccgacgg agggccaggt
      121 gcgagaggcg ctgtccggca acatctgccg ctgcaccggc tacgagagca tcgtcgaagg
      181 agtcctcctg gccgcccggc ggctggcggc cgagagcgga tgacggagcg cggatgacgg
      241 aggcacagcc catggtggga ggccctgttg tggaaggcat ggccgagagg ttcgtcggtt
      301 cgagcgtgag gcgcgtcgag gaccatcgca tcctcaccgg ccgcggccgc tacgtcgacg
      361 acgtcgtcct gcccgccatg gtgcacgccg cattcgtccg cagccccttc ccccacgccc
      421 gcatcctcgg catcgacgtg gctgcggcgc gcgccctcgc cggggtggtg gcggtctaca
      481 cgggcgccga gatggaggag atggtcgtct ccggtcccgg accgctcgcc atgatggtgc
      541 ccggcgggcg cagcccgtcc ttcaccctga tcgccaccga caaggtccgg ctcgtcgggg
      601 acctggtggc gctggtcgtc gccgagagcc gggcggtcgc ggaggacgcg tgcgagctga
      661 tcgaggtcga ctacgacccg ctgcccgccg tggccagcgc ggagcaggcc ctggacccgt
      721 cccggccggc cgtcttcgag gatctcggca gcaacgtcct cgccggcccg accctcgaca
      781 cgtggggaga cgtcgagggg gtgttcgcgt cgtccgacag ggtgcttcgc accaccatcc
      841 gccagcaccg ccaccagaac gtcccgatgg agacccgcgg tagcgtggcg agcttcgacg
      901 ccggtgccgg ccagctcacc gtccacgccg ccacccaggg cgtgggcatg acccaacggg
      961 gcatgagcat gcagctgggc ctgcccatgg agcagatccg tgtgctggcc ggggacatcg
     1021 gggggtcgtt cgggctcaag ttcggcgcgg gccgcgagga catcgccgtg gccgccgcct
     1081 cccgggccct ccaccggccg gtcaagtgga tcgaggaccg caacgagaac ctggtggcat
     1141 cgggccaggc gcgcgaggag tccttcgacg tccaggccgc cttcatggac gacggccggg
     1201 tccgggggct gaaggtgaag atggtgctcg actccggcgc ctacccgggc atgggcgcca
     1261 tgctcggggc catgatcgcc cgcaccatcc cgtcctgcta ccagctcgag gccctcgagt
     1321 tcgagtcgac cacggtggtc accaacaagg ccagctacgt ggcctaccgg gggccgtggg
     1381 cggcggagac cttcgtaagg gagcgcctcc tcgatctcat cgcccgggag ctcgggatgg
     1441 agccgatcga ggtgcgcctg cgcaacgtcg tcacccgtgg ccaggcgccc ctggagatgg
     1501 tcaccggcca gagcctggcg ggcgtcacct cacgccagtc gctggaacgg gtcgccgagc
     1561 tggtcgacgt aggcgccttc cgcgagcggc agcgcggcgc agccgccgag ggacggcacc
     1621 tcggcctggg catggcgacc tacatcgagg cggccccggg tccccgggcc aaggggggcc
     1681 gccgcgggcc gacgggcaac gagctgatgc gcatgcgcct cgagcccgat ggcacggtca
     1741 gcgtcttcac ctcacagatg ccccacggcc agggccacca gaccacgctc gcccaggtgg
     1801 cggccgacga gctcggtatg cccctcgaga tggtcaacgt cgtggtgggc gacagcgacg
     1861 tggtcccggc cgggttcggc accgggggga gccgctcggc cgtgatggca ggcggcaacg
     1921 cgctgcacac cgcccgcaag atccgccagc tggtgctcga gcgggcctcg cagctgatgg
     1981 agatcgacgc cgaggacctc gagatcgtcg gcgacgcggt cgcggcgcga ggcgtccccg
     2041 cccgggcgct gaccctggcc gaggtcgccg tggccgggga cgaccggctg gccggggtgg
     2101 gggggatcct cgaggtcgag aacgcctacg acggtggcga gggcggctgg gcgggcggca
     2161 cccactgcgc catcgtggag
//
LOCUS       DASWGG010000152         4263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1436655, whole genome shotgun sequence.
ACCESSION   DASWGG010000152 DASWGG010000000
VERSION     DASWGG010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436655"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VF954_02710"
     CDS             complement(<1..264)
                     /locus_tag="VF954_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530021.1"
                     /translation="MGAPSVRLRVAATLAVVVVGSAGMSACRKHGTGRPSTSAQLVIV
                     SPTPNEVTGPNVTLQVSLTGAQVVPASTGPLRGDQGHIHVWIGD"
     gene            433..1188
                     /locus_tag="VF954_02715"
     CDS             433..1188
                     /locus_tag="VF954_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9530022.1"
                     /translation="MVQLELVAGDQRPEADAGMRAEAAERRLLALTLMMAEAEHRLKN
                     GLAVVAGWATLLADRWDKVSSEERHRAIEIIRRNSVGLTHEAEDVLRELRAEAATAAL
                     DRVVLDLAAVLEVTVGDWPPGPGHRLELVTDGAAPAWVDPGALQQIVGHLLENAVAYS
                     PGGGRIRVHAYADGGRAVLEVCDQGIGVPDGIDIFAPFSRGTGSQVQAVRGSGIGLYV
                     VRTLVEAMGGEVSARRHPDAGSTFTVRLPARRE"
     gene            complement(1196..1846)
                     /locus_tag="VF954_02720"
     CDS             complement(1196..1846)
                     /locus_tag="VF954_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9530023.1"
                     /translation="MTRVLRHGRVELALHRLADGTGRPLLLLHGLGERSVSEPPEWAA
                     RWAGPVLALDFTGHGSSTMPRAGGYSCEVLMGDVDTVVASEGPLTIHGRGLGAYVALL
                     IAGARPAAVRGAILADGPGLAGGGPGPGSPRLLAVDRAAPAPPDPWALAELSLDVRPP
                     DYATSFARQATHLSGLDSPITVAAVVRPPWLEAILDEPGVAEAPVAAALASYAAVD"
     gene            complement(1843..2763)
                     /locus_tag="VF954_02725"
     CDS             complement(1843..2763)
                     /locus_tag="VF954_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9530024.1"
                     /translation="MTSTADETAITFPGARRPDRSRRVDASGVPIAVYEWGDEAAPPI
                     LLSHGGFDFAATFDLLAPLLADGGWRVVAWDQRGHGDSGHSSLYSWEADMRDAVAVMS
                     SVATRAMPVLGHSKGGALMLELADAAPYRVSHLVNLDGLPSKVTHPDVVDRERTRLKA
                     SELSGWLDFRRQAATNQRKPGTIHELAARRGRMNPRLSQDWLRYIVTIGARQDPDGWR
                     WKFDPLLRMGGFGPWRPQWSLTRTPGLGMPMLGMLGMVHEPMGWATQPDDVQPYLPPG
                     ARIVALEDTGHFVHVERPRVIADAVLEFLS"
     gene            2823..3635
                     /locus_tag="VF954_02730"
     CDS             2823..3635
                     /locus_tag="VF954_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9530025.1"
                     /translation="MSDRVVYTLDGGVADVRLNRPDKINAMDLAMFRALVEVGEEIKA
                     NPAVRAVVLSGEGRGFCAGLDFGIFQAIAGQDGAAVSGESAALAPPTGGRITNTGQQA
                     VYGWTEMPAPVVAAVHGPALGAGCQLALGADIRIVAPDATLSVLEIRWGLTPDMTGTQ
                     MLPRLVGLDVAKELTFTGRTVSGEEAVRLGLATRVSETPRDDALSLAQEIAAKSPHAV
                     RGAKHLLNLAGQVSLAEGFAEEFRTIAGLMGSANQLEAVTAYFEQRAPVFTD"
     gene            3747..>4263
                     /locus_tag="VF954_02735"
     CDS             3747..>4263
                     /locus_tag="VF954_02735"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX9530026.1"
                     /translation="MKAVVEPLEGNRVKLSVDIDEDEFEKAVTSAFARIAREVRVPGF
                     RPGKAPRRLLEARLGKDAGRKEALREALPDYYAQAVRDTDVEPITAPEIDITAGEESG
                     PVSFDAVVQVRPVVAIPGYGGLRTTVPAPAVSEEDIDRQIDRLRSNSAELQVVGRPVK
                     GGDFVTIDLHGT"
BASE COUNT          585 a         1546 c         1579 g          553 t
ORIGIN      
        1 gtcgccgatc cacacgtgga tgtggccctg gtcgccgcgc agcggccccg tcgatgcggg
       61 taccacctgg gcgccggtca ggctcacctg aagggtcacg ttggggccgg tcacctcgtt
      121 tggcgtcggc gagacgatga ccagctgcgc cgaggtggag gggcgcccgg tgccgtgctt
      181 tcggcacgcc gacatcccgg ccgagccgac caccacgacg gccagggtgg ccgcgacccg
      241 gaggcgaacg gagggcgcgc ccatgggccg aagttaccgc cggggcgcac tcggccgctg
      301 gccactcccg ctgaccactc ccaacgggcc accggaaaat gacacgttcg ggcggtttgc
      361 agtcagcgca tcttcaggga ccatgtccat gaccactctg aaccactttg acgagatgcg
      421 gggaccgtgc cgatggtgca gctggaactg gtggcaggcg accagcgacc tgaggccgac
      481 gccgggatgc gtgccgaggc cgcggagcgc cggctgctcg ccctgacgtt gatgatggcc
      541 gaggcggagc accgtctcaa gaacgggctc gccgtcgtcg ccggctgggc gacgctgctg
      601 gccgaccggt gggacaaggt cagcagcgag gagcgccatc gtgccatcga gatcatccgc
      661 cgcaactcgg tgggattgac ccacgaggcc gaggacgtgc tgcgtgagct gcgggccgag
      721 gccgccacgg ccgccctgga ccgggtggtg ctggacctcg ccgcggtcct cgaggtcacc
      781 gtgggcgact ggccgcccgg gccgggtcac cggctcgagc tagtcactga cggtgccgcg
      841 ccggcgtggg tggacccggg cgccctccag cagatcgtcg ggcacctcct ggagaatgcg
      901 gtggcctact ccccgggcgg gggccgcatc cgggtgcacg cctacgccga cggcggccgc
      961 gccgtgctcg aggtctgcga ccagggcatc ggagtgcccg acggcataga catcttcgct
     1021 ccgttcagtc gcggcaccgg cagccaggtg caggcggtgc ggggcagcgg catcgggctg
     1081 tacgtggtgc gcacgctggt cgaggccatg ggcggggagg tgtcggcccg gcgtcatccc
     1141 gacgccgggt cgaccttcac cgtgcggctg ccggcgcgcc gggagtgatg agctttcagt
     1201 cgacggcggc gtagctcgcc agggccgctg cgaccggtgc ctccgccacc cccggctcgt
     1261 ccaggatggc ctccagccac gggggacgga ccaccgccgc cacggtgatc gggctgtcca
     1321 ggccggagag gtgcgtcgcc tggcgggcga agctggtggc gtagtcgggg ggccggacgt
     1381 cgagcgacag ctcggccagg gcccaggggt ccggaggcgc gggcgcggcc cggtccacgg
     1441 ccagcaggcg cggtgagccc gggcccgggc cccctcccgc caggccgggc ccgtcggcca
     1501 ggatggcgcc gcgcactgcg gccgggcggg cgccggcgat cagaagcgcc acgtaggcgc
     1561 cgaggccgcg gccgtggatc gtcagcggcc cctcgctcgc gaccacggtg tcgacgtcgc
     1621 ccatcagcac ctcgcacgag tagccccccg ccctgggcat cgtcgaggac ccgtggcccg
     1681 tgaagtcgag ggccaggacc gggcccgccc agcgagccgc ccactccggc ggctcgctga
     1741 ccgaccgctc cccgagaccg tggaggagca gcaggggcct accggtcccg tcagcgaggc
     1801 ggtgcagggc cagctccacc cggccatggc gcagcacccg ggtcatgaca ggaactcgag
     1861 cacggcgtcg gcgataaccc gcggccgctc gacgtggacg aagtggccgg tgtcctccag
     1921 ggccacgatc cgggctccgg gcggcaggta gggctggacg tcgtcgggct gggtggccca
     1981 acccatcggc tcgtggacca tcccgagcat gcccagcatc ggcatgccca gcccgggcgt
     2041 gcgggtcagc gaccactgcg ggcgccacgg cccgaagccg cccatcctga gcagcgggtc
     2101 gaacttccag cgccagccgt ccgggtcctg cctcgccccg atggtgacga tgtagcggag
     2161 ccagtcctgg gagaggcggg ggttcatccg gccccggcgg gcggccagct cgtggatcgt
     2221 ccccggcttg cgctggttgg tagcggcctg gcggcggaag tcgagccagc cgctcagctc
     2281 gctcgccttg agccgcgtcc gctcgcggtc caccacgtcg gggtgggtga ccttcgacgg
     2341 cagcccgtcg aggttgacga ggtggctcac gcggtagggc gccgcgtcgg ccagctccag
     2401 catgagcgcc ccacccttgc tgtggccgag cacgggcatg gcacgggtcg ccacggagct
     2461 catgaccgcc accgcgtccc gcatgtcggc ctcccagctg tagagcgagg agtgcccgct
     2521 gtcgccgtgg ccccgctggt cccatgcgac gacccgccag ccgccgtcgg cgaggagcgg
     2581 ggcgagcagg tcgaaggtgg ccgcgaagtc gaagcccccg tgactcagca ggatgggcgg
     2641 cgcggcctcg tcgccccact cgtacaccgc gatggggacg cccgacgcgt ccacccggcg
     2701 ggagcggtcc ggccggcggg cgcccgggaa ggtgatcgcc gtctcatcgg cggtgctcgt
     2761 catgcgccga gggtgtccca gccgggctcg ccgccgcaac tcaccgctgc cagactggcg
     2821 ccatgtcgga ccgtgtcgtc tacaccctcg acggtggcgt ggcggacgtg cgcctcaacc
     2881 gcccggacaa gatcaacgcc atggacctgg ccatgttccg ggccctggtc gaggtcggtg
     2941 aggagatcaa ggccaacccg gcggtccgcg ccgtcgtgct gtccggcgag ggccggggct
     3001 tctgcgccgg cctcgacttc ggcatcttcc aggccatcgc cggccaggac ggcgcggccg
     3061 tgtcgggaga aagcgccgcg cttgccccgc caaccggggg ccgcatcacc aacaccggcc
     3121 agcaggcggt gtacgggtgg acggagatgc cggccccggt cgtggcggca gtgcacggcc
     3181 ccgccctcgg ggccggatgc cagctggccc tcggcgccga catccggatc gtggcgcccg
     3241 acgccaccct gtcggtcctc gagatccgtt ggggcctgac ccccgacatg acgggcacgc
     3301 agatgctgcc ccgtctcgtc gggctcgacg tggccaagga gctgaccttc accgggcgaa
     3361 cggtgagcgg tgaggaagcc gttcgcctcg gcctggccac ccgcgtgtcc gagaccccgc
     3421 gcgacgacgc cctgtcgctg gcgcaggaga tcgcggccaa gagcccgcac gccgtccggg
     3481 gcgccaagca tctgctcaac ctcgccggcc aggtgagcct ggccgagggc ttcgccgagg
     3541 agttccgcac catcgccggc ctcatgggct cggccaacca gctcgaagcc gttacggcct
     3601 acttcgagca gcgggcgccg gtcttcaccg actgaggctc gggcaggggt ccgccggccg
     3661 cgccggccgc gttttccccg ctccacccga cctccagtgg agtggtgcgc ccacttcggg
     3721 caccggccgg tacagtgggc gccggtatga aagccgtcgt cgagcccctc gagggcaaca
     3781 gggtcaagct ctcggtcgac atcgacgagg acgagttcga gaaggcggtg acgtccgcct
     3841 ttgcccggat tgcccgggag gtccgcgtcc ccgggttccg gccgggcaag gcgccccggc
     3901 ggctgctcga agcccgcctg ggcaaggacg ccggccgcaa ggaggcgttg cgggaggcgc
     3961 tccccgacta ctacgcccag gccgtgcgtg acaccgacgt cgagcccatc accgcccccg
     4021 agatcgacat caccgcaggc gaggagtcgg gcccggtgag cttcgacgcg gtggtccagg
     4081 tccgaccggt cgtcgccatc cccggctacg gcggcctgcg gaccaccgtc cccgctccgg
     4141 ccgtgtcgga ggaggacatc gaccgccaga tcgaccgtct gcgctccaac tcggcggagc
     4201 tccaggtcgt cgggcggccg gtcaagggcg gggacttcgt gaccatcgac ctgcacggca
     4261 ccc
//
LOCUS       DASWGG010000153         9372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_797259, whole genome shotgun sequence.
ACCESSION   DASWGG010000153 DASWGG010000000
VERSION     DASWGG010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797259"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VF954_02740"
     CDS             complement(<1..520)
                     /locus_tag="VF954_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530027.1"
                     /translation="MGLTHPHAMPGRRRAVSVALAAAVLAAVPVWLAARRGGRASADD
                     TATITAGYRIAGSDGSAQPSGGAAGPGAPGPGAWGPGAPGGGAAGLGAASGPGNGPGP
                     AARNIEPTPQGGGSLEASPPSGPPVSPVASVAATQSDPARRGFVTRVGTGFQLDGWPY
                     RFDGMNIYDVNSN"
     gene            complement(621..4076)
                     /locus_tag="VF954_02745"
     CDS             complement(621..4076)
                     /locus_tag="VF954_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9530028.1"
                     /translation="MDTLAPSGGERLILLTRRIWSSDHLSVGQLRYSRPAAAVAVTYL
                     AWAALDLGGDHTTHEMARAMVVVVSAGMVWACTRAARGSEGRFRRGWYFLAAAALCWL
                     VSNGVAGVREVLAHTELAVPTPGDGASLLAVLLQVMGFLAFLDPAATGAGRLRRLTDG
                     LLIASATVFIGWAAFLERAYRQGGNGPGGIVALAYPVSHVVLIALALTVLLRMPAGCK
                     RPAWLLLAGQFALAVSFSAFAYLQIAARYLPGSPETFGWIVGALLLVVAAGLGVEETP
                     PPASAPADDLVVTARGRLEIALPFAPVLVAAAMAAARERSGHLSSFLLVDAGVILLLL
                     LVRQVLAQLDNLELAKSLDHRIKTRTGELHMRERQFRSLVQNATDVLTLVDAGGIICY
                     QSDSVARVLGFATGALLGRPVADLLHPDERSELLERLRGAQPNSPALMTEMHLLRADG
                     TWCWTDTTVSNLLGDAAVRGILLTSRDVGDRRRLEDELRRQALHDPLTGLGNRTVLYD
                     RLSHAIRRASRSPQEVALLLLDLDGFKAVNDTLGHGAGDSLLVEVGSRLQDSVRPGDT
                     VVRMGGDEFAILLEQAEADAAILVAQRVINRLRAPFVVAGTPIVVNGSVGIAHGSTAG
                     ASAEDLMRRADLAMYAAKARGKNRYQVFEAEMQHDARARVELETDLRRALSEGELRVH
                     YQPLVDIPSGRIGGAEALVRWQHPVQGLIHPAVFIPVAEESDLVLRLGRLVLNEACRQ
                     ARRFQELYPTDPPFSISVNVATRQLTSEWLVDEVKTALDYSGLDPDCLVLEITEGALM
                     EHASKTIATIERLRALGVRLAIDDFGTGYSSLSRLRSFPVDELKIDRAFVKEIVSSSD
                     EAPLVAAIVAMGHSLRLTIVAEGVETLEQLTCLHLHGVESVQGYLLSRPISADAFEAM
                     LSHPTGLLEPVTAGDGMADGVAGLGGPDDDLSSARKGFMDVVSQAARTATQAPVAEVV
                     EVVEPVLAELARVTGLDQVYLIEVREQDPSSGAALQVVAAHSWGSVGVPVGSPMPWPG
                     SPGGRLLPDGGIIGDLSAPAPAPAPAPDAYAEVAGLDRLWSEFGLRTFVTVPVRDTDG
                     RLVGALCGVSGRPEAADMSIKVLFELFAGLLTEHVGRLTTHGGEQEGWLPAGTPGPGR
                     ALAAS"
     gene            4142..4993
                     /locus_tag="VF954_02750"
     CDS             4142..4993
                     /locus_tag="VF954_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX9530029.1"
                     /translation="MLKLLRRIKNYFTASATQKFNERADPKVQLEQAIGEAQEQHRRL
                     TEQAANVIANQKQTEMRLNRAMEDMGKANGSARQAVVMADEAGKRGDGAKAVEYTRAA
                     ESFANRLVTIEKEVESLKTLHLQSAQAADQAKQAVAQNSAALQQKLTERQKLLSQLDQ
                     AKMQEQMNKAMATLSLEVGQDVPTFDEVRDKIEARYAKALGQSELQGATVETRMLEVE
                     QATMHSEAAARLDQIKVQLGLATGTSELGGGAEGGELGVGGATAATGAGASEPRPAAT
                     EDGAPGA"
     gene            complement(5094..6191)
                     /gene="proB"
                     /locus_tag="VF954_02755"
     CDS             complement(5094..6191)
                     /gene="proB"
                     /locus_tag="VF954_02755"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX9530030.1"
                     /translation="MIVVAKIGTSSITGAAGEIDQAAVAGLCAEVAGLRRSGHRAVVV
                     TSGAIAAGLPPLGLGGRRPTDLATLQAVAAVGQHHLMRVYEAALSTHSLLGGQILLAP
                     LDFVHRQQYLHARQTLTRLLDLGVVPIVNENDAVADDEIRFGDNDRLAALVAHLVSAE
                     LLVLLTDTPGLYTADPRLDSGASLIEEIVELDHELGVAAGGPGSARGSGGMVSKLAAA
                     KMAAWSGVRTVIAAAGRAGVLGDAVAGVQGVGTVIQPHERRLPARKLWIAFAVGSSGT
                     IVVDEGARRALASGGRSLLPAGVVDVGGDFEVDDAVEIAGPDGRVFAKGLARHRSALV
                     RQWRGSQTAALPPDVPHEVVHRDDLVVLPVH"
     gene            complement(6199..7437)
                     /gene="obgE"
                     /locus_tag="VF954_02760"
     CDS             complement(6199..7437)
                     /gene="obgE"
                     /locus_tag="VF954_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX9530031.1"
                     /translation="MPGFVDEAQVHVKAGDGGAGSVSFRREAHVPRGGPDGGDGGSGG
                     GVWLVADHDVASLLAFRDHPHRRAPSGGHGQGKGRHGATGPDLEVKVPEGTVVRDQAG
                     LALADLVRHGDRWLAASGGRGGRGNSRFLANRRRAPSFAEQGEVGEEAWLNLELKLMA
                     DVALVGFPNAGKSTLISRISAAKPKIADYPFTTLQPHLGVVRFGEHELVVADVPGLIE
                     GAAEGRGLGHRFLRHVERARVLALLLDLAPADGPSPAGQERVLLTELGRYRPELLERP
                     RVVVGSKADLAPPDADFDGMRISAVTGEGISRLLGELAGVVHAARAALPDAPPVVVHR
                     PVPDEVRVARDDDGTFVVRGRAAERAVAVNDLNNLQALAYVQHRLKRLGVDRALARAG
                     AREGDAVRIGPFTFDYEPDR"
     gene            complement(7480..7743)
                     /gene="rpmA"
                     /locus_tag="VF954_02765"
     CDS             complement(7480..7743)
                     /gene="rpmA"
                     /locus_tag="VF954_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX9530032.1"
                     /translation="MSKTKGGGSTRNGRDSRAQRLGVKVFDGTAITAGAIIVRQRGTR
                     FHPGLNVGRGGDDTLFALSPGQVKFGHRKGRRLVDVLPAADQA"
     gene            complement(7755..8063)
                     /gene="rplU"
                     /locus_tag="VF954_02770"
     CDS             complement(7755..8063)
                     /gene="rplU"
                     /locus_tag="VF954_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX9530033.1"
                     /translation="MYAVIKTGGKQERVAEGQRVRVERLPQGAGEAVSFAPVLLVDGP
                     TVVAGADLHGATVSARVLGEVKGPKITGFTYKPKTNQRRRWGHRQHYSEIEITGISRG
                     "
     gene            complement(8164..>9372)
                     /locus_tag="VF954_02775"
     CDS             complement(8164..>9372)
                     /locus_tag="VF954_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX9530034.1"
                     /translation="SLIEYYVSRPQDDVNQIDGNIYIGRVQNVLPGMEAAFVDIGTPK
                     NAVLYRGDVRYDTDDVEGGGRAARDAVRIEHLLRPGQVILCQVTKNPIGAKGARLTQE
                     VSLPGRFVVLIPNSDTYGISKRLPDDERKRLRRILDDVRPSGHGLIVRTAAEGATPDE
                     LSRDVARLVRQWNAIDTQAARSAAAALLYREPDMAVRVIREEFNKEYRGIILDDPGLH
                     QEVQEYVASISPELADRVELYDPAREGLPLLERFHVHEQLHKALDRKVWLPSGGSLII
                     ERTEALTVIDVNTGKNVGTSNLEETVYRNNLEAAEEIARQVRLRDIGGIIVIDFIDME
                     IRHNRDEVIKAFRAALGRDKTRTQVFDISELGLVEMTRKRISEGLVESMSRTCPMCAG
                     RGILIDETLL"
BASE COUNT         1333 a         3377 c         3452 g         1210 t
ORIGIN      
        1 cgttggagtt gacgtcgtag atgttcatcc cgtcgaagcg gtagggccaa ccatcgagct
       61 gaaacccggt gccaacgcgg gtcacgaagc cacggcgggc cggatccgac tgggtggctg
      121 cgaccgacgc gaccgggctg acgggcgggc cggagggcgg gctggcttcg agcgacccac
      181 cgccttgcgg cgtcggctct atgttccgcg cggccgggcc cgggccgttg ccgggcccgg
      241 aggcggcgcc aaggccggcc gcgcctccgc cgggcgcgcc cgggccccac gcgcctgggc
      301 ccggcgctcc tgggccggcc gcgcctccgg acggctgggc cgacccgtcg gaccccgcga
      361 tccggtaccc cgccgtgatg gtggccgtgt cgtcggcgct ggccctgccg ccgcggcgcg
      421 ccgccagcca caccggaacg gcggccagga ccgccgccgc caacgcgacc gacaccgcgc
      481 ggcgccgccc cggcatcgcg tgcgggtgcg tcaggcccac gtctcctccc ggccggccgc
      541 gccgtgcggc cctttggatg tgtttcggcc aaagcgtccg attcccttag ccggtctccg
      601 atcctacggg gcgcgtgcgt ctacgacgcg gcgagtgccc gccccggccc gggggtgccc
      661 gccggcagcc acccctcctg ctcgccgccg tgcgtcgtga gccgcccgac gtgctcggtc
      721 aacagccctg cgaagagctc gaacaacacc ttgatcgaca tgtcggccgc ctcggggcgc
      781 cccgacaccc cgcagagggc gccgaccagc cgcccgtcgg tgtcccgcac agggacggtc
      841 acgaaggttc gcagcccgaa ctccgaccac aggcgatcga ggcccgctac ctcggcgtag
      901 gcgtctggcg cgggcgccgg cgccggcgcc ggcgccgaga ggtcgccgat gatcccgccg
      961 tccgggagga gccggccgcc gggcgagccc ggccacggca tcgggctccc gacggggacc
     1021 cccaccgagc cccaggagtg ggcggccacg acctggaggg cagcgccgct ggaaggatcc
     1081 tgctcacgaa cctcgatcag atagacctgg tccagcccgg tcacccgggc cagctcggcc
     1141 aggacaggct cgacgacctc gacgacctcg gccacgggag cctgggtggc ggtacgggcc
     1201 gcctggctca ccacgtccat gaagcccttt cgggcactgg acaggtcgtc gtcggggccc
     1261 ccgaggccgg cgaccccatc cgccatgccg tcccctgcgg tcacgggctc gagcagtccg
     1321 gtcgggtggg acagcatggc ttcgaaggcg tcggccgaga tgggcctgga cagcaggtac
     1381 ccctgcaccg actcgacccc gtgcaggtgg aggcaggtga gttgctctag ggtctccacg
     1441 ccctcggcca cgatcgtcag ccgcaagctg tggcccatgg cgacgatggc ggccacgagc
     1501 ggggcctcgt cgctggacga gacgatctcc ttcacgaagg cccggtcgat cttgagctcg
     1561 tccaccggga acgagcggag ccggctcagc gacgagtacc ccgtcccgaa gtcgtcgatg
     1621 gccaggcgca cgcccagggc ccgaagccgc tcgatggtcg caatggtctt gctggcgtgc
     1681 tccatcaggg cgccctcggt gatctccagc acgaggcaat cggggtccag cccgctgtag
     1741 tcgagggccg tcttcacctc gtcgaccagc cactcggacg tgagctggcg ggtggcgacg
     1801 ttgacgctga tcgaaaaggg cggatcggtg gggtacagct cctggaaccg cctggcctgg
     1861 cggcacgcct cgttgagcac caggcggccg aggcgcagca cgagatcgct ctcctcggcg
     1921 acagggatga agacggcggg atggatcagg ccctgcaccg ggtgctgcca ccggaccagg
     1981 gcctcggcac ccccgattcg ccccgacggg atgtcgacga gcggctggta gtggacgcgg
     2041 agctcgccct ccgacagggc ccgccggaga tctgtctcga gctcgacccg ggcccgggcg
     2101 tcgtgctgca tctcggcctc gaacacctgg tagcggttct tcccgcgggc cttggccgcg
     2161 tacatggcca ggtcggcccg gcgcatgagg tcctcggcgc tcgcaccggc cgtcgagcca
     2221 tgggcgatgc ccacgctccc gttcacgacg atgggcgtcc cggcgaccac gaacggggcc
     2281 cggagccggt tgatgacccg ctgggccacg aggatggccg cgtcggcctc tgcctgctcg
     2341 agcaggatgg cgaactcgtc gccgcccatc cgcaccaccg tgtcgcccgg cctgacgctg
     2401 tcctggagcc gactgcccac ctcgaccagc aggctgtcgc ccgcgccgtg acccaacgtg
     2461 tcgttgacgg ccttgaagcc gtccaggtcg agcaggagca gcgccacctc ctggggcgac
     2521 cgggaagcgc gccggatggc gtggctcaac cggtcgtaca gaactgtccg gttgccgagg
     2581 ccggtgaggg ggtcgtggag ggcctggcgg cgcagttcgt cctccagccg gcgccggtcg
     2641 cccacgtcgc ggctggtgag caggatgccc cgtacggcgg cgtcgcccag caggttggag
     2701 acggtggtgt cggtccagca ccatgtcccg tccgctcgca gcaggtgcat ctcggtcatg
     2761 agggcagggc tgttgggctg ggcgccgcga agccgttcga gcagctcgga gcgctcgtcc
     2821 ggatggagca gatcggcgac cggccggccg agcagcgcgc cggtggcgaa cccgaggaca
     2881 cgggccaccg agtcgctctg gtagcagatg atgccgcccg cgtcgacgag ggtgaggacg
     2941 tcggtggcgt tctgcacgag cgagcggaac tggcgctccc gcatgtgcag ctcgccggtg
     3001 cgggtcttga tccggtggtc gaggctcttg gccagctcca ggttgtcgag ctgggcgagc
     3061 acctgccgga cgagcagcag cagcaggatc acgccggcgt cgacgaggag gaaggacgac
     3121 aggtgcccgc tgcgctcccg cgccgccgcc atggccgcgg ccacgagcac gggcgcgaag
     3181 ggcagggcga tctccaaccg gcctcgcgcc gtcacgacca ggtcgtcagc gggggccgag
     3241 gcgggtggcg gcgtctcctc cacgcccagg ccggccgcca ccaccaggag cagggcaccg
     3301 acgatccagc cgaaggtctc cgggctcccc ggcaggtacc gcgccgcgat ctggaggtag
     3361 gcgaaggccg agaaggacac ggccagggcg aactggccgg ccaggagcag ccacgccggc
     3421 cgtttgcaac cggccggcat ccgcaacagc accgtgaggg cgagcgcgat gagcaccacg
     3481 tggctgacgg ggtaggccag agcgacgatc ccgccgggcc cgttcccgcc ctgacggtag
     3541 gcccgctcca ggaaggcggc ccagccgatg aacacggtcg ccgacgcgat cagcaggccg
     3601 tcggtcaggc ggcgcagccg gccggccccg gtggctgcgg ggtcgaggaa ggcgaggaac
     3661 cccatcacct gcaagagcac ggcgagcagc gacgctccgt cgcccggggt cggcacggcg
     3721 agctcggtgt gggcgaggac ctcccggacg ccggccacgc cattggacac gagccagcac
     3781 agcgcggccg cggcgaggaa gtaccaaccc cggcgaaagc ggccctcgct gccccgcgcc
     3841 gcccgcgtgc acgcccacac catgccggcc gacaccacga cgaccatcgc cctcgccatc
     3901 tcgtgggtgg tgtggtcgcc cccgaggtcg agcgcggccc acgccaggta tgtgacggcg
     3961 acggccgcgg caggacgcga gtagcgcaac tggcccacgg acaggtggtc ggaactccag
     4021 atgcggcgcg tgagcaaaat gagcctctcc ccacccgatg gggccagggt gtccatggcc
     4081 cactcgggcg cggggccatg cccgggacac tgggcggtgc cggcggcgta gcatcacggg
     4141 catgttgaag ctgctacggc gcatcaagaa ctacttcacg gcttcggcca cgcagaagtt
     4201 caacgagcgg gcagacccca aggtccagct cgagcaggcc atcggcgagg cgcaggagca
     4261 gcaccggcgc ctgaccgagc aggccgccaa cgtgattgcc aatcagaagc agaccgagat
     4321 gcggctcaac cgcgccatgg aggacatggg gaaggccaac ggctcggccc gccaggccgt
     4381 ggtcatggcc gacgaggccg gcaagcgggg agacggcgcc aaggcggtcg agtacacgcg
     4441 ggcggccgag tccttcgcca accggctggt gaccatcgag aaggaggtgg agtcgctgaa
     4501 gaccctccac ctgcagtccg ctcaggccgc cgaccaggcc aagcaggccg tggcccagaa
     4561 ctcggcggcg ctccagcaga agctgaccga gcgccagaag ctgctctccc agctggatca
     4621 ggccaagatg caggagcaga tgaacaaggc gatggccacc ctctccctgg aggtcggcca
     4681 ggacgtcccc accttcgacg aggtccgcga caagatcgag gcgcgctacg ccaaggccct
     4741 gggccagtcc gagcttcagg gcgccaccgt cgagacccgg atgctcgagg tggagcaggc
     4801 gacaatgcac agcgaggcgg ccgcccggtt ggaccagata aaggtccagc tcgggctggc
     4861 gacgggcacg tctgagctcg gcggcggcgc cgagggcggc gagctgggag tgggcggggc
     4921 caccgcggcc acaggggcgg gtgccagcga gcccaggccc gcggcgaccg aggacggcgc
     4981 cccgggggcg tgaccgcctc ggatggtccg ggcccgcgcc gccgggcacg gccgccggcg
     5041 gggctggggt ccccgcggtg cggaggaggc cggggttggg gccccggccg ggcctaatgg
     5101 accggcagca cgacgagatc gtcgcggtgg accacctcgt ggggcacgtc gggaggcagg
     5161 gcggccgtct ggctcccccg ccactggcgc acgagcgcgc tgcgatgacg ggccagcccc
     5221 ttggcgaaca cccggccgtc cggcccggcg atctccacgg cgtcgtcgac ctcgaagtcc
     5281 ccgccgacgt cgaccacgcc cgccggcagc agcgaccggc cccccgaggc cagggcccgg
     5341 cgggcgccct cgtcgacgac gatggtcccc gacgagccca ccgcgaaggc gatccacagc
     5401 ttgcgggccg gcaggcgccg ctcgtgcggc tggatcacgg tcccgacgcc ttgaacgccg
     5461 gccacggcgt cgcccagcac gccggcccgg ccggccgccg cgatcacggt ccggaccccc
     5521 gaccacgccg ccatcttggc cgcggccagc ttggagacca tgcccccgct accccgggcg
     5581 ctgcccggcc cgccggcagc gaccccgagc tcgtggtcga gctccacgat ctcctcgatg
     5641 agcgatgccc ccgagtccag gcgggggtcg gcggtgtaga ggccgggcgt atcggtcagc
     5701 agcacgagca gctcggcgct caccaggtgg gccacgagcg cggccaggcg gtcgttgtcg
     5761 ccgaagcgga tctcgtcatc ggcgacagcg tcgttctcgt tgacgatcgg caccacgccg
     5821 aggtcgagga gccgggtgag cgtctggcgg gcgtgcaggt actgctggcg gtggacgaag
     5881 tccagcgggg cgaggaggat ctggccgccg agcagggagt gcgtggagag ggccgcttcg
     5941 tacacccgca tgaggtggtg ctggcccacg gccgccaccg cctggagggt ggccaggtcg
     6001 gtgggccgcc gcccgcccag cccgaggggc gggagcccgg ccgcgatggc cccggacgtg
     6061 acgaccacgg cgcggtggcc gctccggcgc aggccggcga cctcggcgca cagccctgcc
     6121 acggccgcct ggtcgatctc gccggcggcg ccggtgatcg acgacgtgcc gatcttggcg
     6181 accacgatca ccggcggctc accggtcggg ctcgtagtcg aaggtgaacg gcccgatccg
     6241 caccgcgtcg ccctcccggg cgccggcgcg ggcgagagca cggtccaccc cgagccgctt
     6301 gaggcggtgc tggacgtagg cgagggcctg gaggttgttg agatcgttga cggccacggc
     6361 ccgctcggcc gcccggcccc gcaccacgaa ggtcccgtcg tcgtcccggg ccaccctcac
     6421 ctcgtcgggc acgggccggt ggaccaccac gggcggcgcg tccggcaggg cggcccgggc
     6481 ggcgtgcacc acgccggcga gctcgcccag caggcgcgag atgccctccc cggtgaccgc
     6541 cgagatccgc atcccgtcga agtcggcgtc cggcggggcc aggtcggcct tcgacccgac
     6601 cacgacccgc ggccgctcga gcagctcggg ccggtagcgc cccagctccg tgagcagcac
     6661 ccgctcctgc ccggcggggc tggggccgtc cgccggggcc aggtcgagca ggagggccag
     6721 cacccgggcc cgctcgacgt ggcggaggaa acgatggccc agccccctgc cttcggccgc
     6781 cccctcgatg agaccgggga cgtcggccac caccagctcg tgctcgccga agcgcaccac
     6841 gcccagatgg ggctggaggg tcgtgaacgg gtagtcggcg atcttgggct tggccgcgga
     6901 tatgcgggag atgagcgtgc tcttccccgc gttcgggaag ccgacgaggg cgacgtcggc
     6961 catgagcttg agctccaggt tgagccaggc ctcctccccg acctcgccct gctcggcaaa
     7021 agacggcgcc cggcgccggt tggccaggaa gcggctgttg ccccgcccgc cccggccgcc
     7081 gctcgccgcc agccagcggt cgccgtggcg cacgagatcg gcgagggcca ggccggcctg
     7141 gtcgcgcacg accgtgccct cggggacctt gacctcgagg tcggggccgg tggcgccgtg
     7201 gcggcccttg ccctggccgt ggccgccgct cggcgcccga cggtgcgggt ggtcccggaa
     7261 cgcgagcagc gaggccacgt cgtggtcggc gaccagccac acgcccccgc ccgacccgcc
     7321 gtcgcccccg tccgggccgc ccctggggac gtgggcctcg cgccggaacg acaccgatcc
     7381 ggccccgccg tcaccggcct tgacgtggac ctgggcctcg tcgacgaacc cgggcatggg
     7441 agaaggggcg gccggggcgc cggcgcccgc cgtggcttct tacgcctggt cggccgcggg
     7501 gaggacgtct accagccgcc ggcccttgcg gtggccgaac ttgacctggc cgggcgagag
     7561 cgcgaacagg gtgtcgtcgc cgccccggcc gacgttgagg ccggggtgga accgcgtgcc
     7621 ccgctggcgc acgatgatgg caccggcggt tatggcagtg ccgtcgaaca ccttcacgcc
     7681 gaggcgctgg gctcgggagt cccgcccgtt gcgggtggag ccgccacctt tggtcttgga
     7741 catctcgctc ctacctagcc ccggctgatg ccggtgatct cgatctcgct gtagtgctgg
     7801 cgatgacccc accgccggcg ctggttggtc ttgggcttgt aggtgaagcc cgtgatcttg
     7861 ggccccttca cctcgccgag cacgcgggcc gagacggtcg cgccgtgcag gtcggcgcca
     7921 gccacgaccg tcgggccgtc gaccaggagg accggggcga aggagacggc ctcgccggcg
     7981 ccctggggca gccgctccac ccggacgcgc tggccctcgg ccacccgctc ctgcttgcca
     8041 cccgtcttga tcaccgcata cataggacgc gcagtgtacc cgtcactcag gacccggcgc
     8101 cttcctggca ccgaaaggtg gtgttttcca cgcctttcgg tgccaagaac gggcccccac
     8161 gccctacagg agggtctcgt cgatgaggat gccccggccg gcgcacatgg ggcaggtccg
     8221 ggacatggac tcgaccagcc cctcggagat gcgtttgcgg gtcatctcca ccagcccgag
     8281 ctcggagatg tcgaagacct gcgtcctggt cttgtcccgc ccgagggcgg cccggaaggc
     8341 cttgatgacc tcgtcgcggt tgtggcggat ctccatgtcg atgaagtcga tcacgatgat
     8401 ccccccgatg tcgcggagcc ggacctgccg ggcgatctcc tcggcagcct ccaggttgtt
     8461 gcggtagacg gtctcctcca ggttggaggt gccgacgttc ttgcccgtgt tgacgtcgat
     8521 gacggtgagg gcctcggtgc gctcgatgat gagcgagcct cccgatggca gccagacctt
     8581 gcggtcgagc gccttgtgga gctgctcgtg gacgtggaac cgctccagga gcggtaggcc
     8641 ctcccgggcc gggtcgtaca gctccacccg gtcggcgagc tcggggctga tgctcgccac
     8701 gtactcctgc acctcctggt ggaggccggg gtcatcgagg atgatcccgc ggtactcctt
     8761 gttgaactcc tcccggatca cccggacggc catgtccggc tcccggtaga ggagggcggc
     8821 ggcggcgctg cgagccgcct gggtgtcgat ggcgttccac tgccgcacga gccgggccac
     8881 gtcgcggctc agctcgtcgg gggtcgcccc ctcggcggcg gtccggacta tgagcccgtg
     8941 gcccgagggg cggacgtcgt cgaggatgcg acggaggcgc ttgcgctcgt cgtcggggag
     9001 acgcttggag atgccatagg tgtcgctgtt ggggatgagc accacgaacc ggccggggag
     9061 cgacacctcc tgggtgaggc gggcgccctt ggcgccgatc gggttcttgg tgacctggca
     9121 gaggatgacc tggcccgggc ggagcaggtg ctcgatccgg accgcatcgc gcgccgcccg
     9181 gcccccgccc tcgacgtcgt cggtgtcgta tcggacgtcg ccccggtaga gcaccgcgtt
     9241 cttgggcgtg ccgatgtcga cgaacgccgc ctccatgccg ggaaggacgt tctggacccg
     9301 cccgatgtag atgttgccgt cgatctggtt gacgtcgtcc tgcgggcgcg acacgtagta
     9361 ctcgatgagg ga
//
LOCUS       DASWGG010000154         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_797420, whole genome shotgun sequence.
ACCESSION   DASWGG010000154 DASWGG010000000
VERSION     DASWGG010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797420"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VF954_02780"
     CDS             complement(<1..1121)
                     /locus_tag="VF954_02780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9530035.1"
                     /translation="MKAHTPGRLGSAGVVLTALAALAAPALAASPPAGASTPPANAPS
                     LQQRKAQANQQRQAAQAQLDLARANDDQVQAEVTRLDAAVVSEATKVAGADQALDTAK
                     REVRSAQAAVNALRARTDRARRSLTAVAVSGYMSPPAEDAVTQILTAADLQQAARRQA
                     FLVNVQATTTEVIDALRQAQQDETAAAERLQQVQSTAQARARAEEATQLELRAQQVAQ
                     AAAHSELQKRIAELLGETQAYAAQESQLEALITAAQAAARSVTGPVSNIGLIWPVHGP
                     VTSEFGPRWGGFHPGIDIGAGYGAPIHAAKAGTVIFAGYYGGYGNFVVIDHGGAVATA
                     YAHQSRIAVSQGQTVQQGQVIGYIGSTGYSTGPHLHFEVR"
     gene            complement(1345..>1802)
                     /locus_tag="VF954_02785"
     CDS             complement(1345..>1802)
                     /locus_tag="VF954_02785"
                     /inference="COORDINATES: protein motif:HMM:NF019528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX9530036.1"
                     /translation="IDGFAGLNNHRGKTRTVSHGDYRLDNMLFGTADGGYPLAVVDWQ
                     TVSWGEATADASYFLGAGLLPADRAAHERDLLRVYHEGLGAYGIDGYSWDECWADYRR
                     HAFGGYVMAVVASQLVHDTDRGREMFAVMAERHAAQALDLESETFFGPS"
BASE COUNT          230 a          623 c          688 g          261 t
ORIGIN      
        1 cggacctcga agtggagatg gggcccggtc gagtagccgg tcgagccgat gtagccgatg
       61 acctggccct gctggacggt ctggccttga ctgacggcga tccgggattg gtgggcgtac
      121 gcggtggcga ccgcgccgcc gtggtcgatt accacgaagt tgccgtagcc cccgtagtag
      181 ccggcgaaga tcaccgtgcc cgccttggcc gcgtggatcg gcgccccgta gcccgcaccg
      241 atgtcgatgc cggggtggaa cccaccccat cggggcccga actcggaggt gaccgggccg
      301 tgcaccggcc agatcagccc gatgttggac accgggccgg tcacggaccg ggccgcggcc
      361 tgggccgcgg tgatcagggc ctcgagctgc gactcctggg cggcgtaggc ctgggtctcc
      421 ccgaggagct cggcgatgcg cttctggagc tcggagtggg ctgcggcctg ggcgacctgc
      481 tgggcccgga gctcgagctg ggtcgcctcc tcggctcggg cccgggcctg ggccgtggat
      541 tgcacctgct ggaggcgttc ggcggcggcc gtctcgtcct gctgggcctg gcggagggcg
      601 tcgatcacct cggtcgtggt ggcctggacg ttgaccagga acgcctggcg ccgggcggcc
      661 tgttgcaggt cggccgcggt caggatctgg gtcacggcgt cctcggccgg cgggctcatg
      721 tagccggaca ccgccaccgc cgtgagcgaa cgtcgcgccc gatcggtcct ggctcggagc
      781 gcgttcacgg cggcctgggc gctgcgcacc tcccgtttgg cggtgtcgag ggcctggtcg
      841 gccccggcca ccttcgtggc ctcgctgacc accgcggcgt cgaggcgggt gacctcggcc
      901 tgcacctggt cgtcgttggc gcgggccaga tcgagctggg cctgggcggc ctgtcgctgc
      961 tggttggcct gggccttgcg ctgctggagc gagggggcgt tcgcgggcgg ggtgctggcg
     1021 cctgcgggag gggaggctgc gagcgcgggc gcggcgaggg cggccagcgc cgtgagcacc
     1081 accccggccg accccagccg ccccggggta tgggccttca tgcccggctc atccgggggt
     1141 cgtcccgcgg agtgatcccc gggaccaacc ggggccaacc cggggccaac cccggggcca
     1201 accccggggc caaccccggg gccgagcgga tgccacacgc acccctcact tgtacaggga
     1261 ccacagcagg cacagcaaca acagtgccat cagtcgccac cgagcgtgcg cacccggcgc
     1321 ctctacgagg cctctacgag gcctctaaga ggggccgaag aacgtctcgc tctcgagatc
     1381 gagggcctgg gcggcgtgcc gttccgccat caccgcgaac atctcgcggc cccggtcggt
     1441 gtcgtggacg agctgggagg cgaccacggc catcacatag ccgccgaagg cgtggcggcg
     1501 gtagtcggcc cagcattcgt cccacgagta cccgtcgatc ccgtacgcgc ccagcccctc
     1561 gtggtacacg cgcagcaggt cgcgttcgtg ggccgcccgg tcggccggga gcaggccggc
     1621 accgaggaag tacgaggcgt cggcggtcgc ctcgccccac gagacggtct gccagtcgac
     1681 gacggcgagg gggtacccgc cgtcggccgt gccgaagagc atgttgtcca accggtagtc
     1741 gccgtgggag accgtgcggg tcttgccccg atggttgttg aggccggcga aaccgtcgat
     1801 ca
//
LOCUS       DASWGG010000155         5993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_799294, whole genome shotgun sequence.
ACCESSION   DASWGG010000155 DASWGG010000000
VERSION     DASWGG010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799294"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..372)
                     /locus_tag="VF954_02790"
     CDS             complement(136..372)
                     /locus_tag="VF954_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530037.1"
                     /translation="MKVARQLLLAAVLSAGLAACSGGSGPASAPTATTQPPAPGANVD
                     PNAHGVITDPINRAKTVVTQLNQQQSQTDGAGNS"
     gene            565..2262
                     /locus_tag="VF954_02795"
                     /pseudo
     CDS             565..2262
                     /locus_tag="VF954_02795"
                     /inference="COORDINATES: protein motif:HMM:NF005860.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
     gene            2292..2825
                     /gene="ilvN"
                     /locus_tag="VF954_02800"
     CDS             2292..2825
                     /gene="ilvN"
                     /locus_tag="VF954_02800"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX9530038.1"
                     /translation="MPQTTGHHILSVLVENKAGVLARVASLFSRRGYNIYSLAVAPTD
                     DERFSRITIVVDAESAPLEQITKQLFKLINVIKISELDPAEAVERELLMATVRAEPGT
                     RGQVIELVQVFEGKIVDVGQDELTVMLAGTPAKLDDFEDLMRPYGINELQRTGRVALP
                     RLERQAPRLRSVTGKVS"
     gene            2829..3854
                     /gene="ilvC"
                     /locus_tag="VF954_02805"
     CDS             2829..3854
                     /gene="ilvC"
                     /locus_tag="VF954_02805"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX9530039.1"
                     /translation="MASIYYDKDADPALIAGRKVAILGYGSQGHAHALNLRDSGVDVR
                     VGLRQGSASAAKASSAGLRVLPTDQAAAEADLIMMLLPDTEQAAVYTDAVAPRLAPGD
                     AVFFAHGFNIRFGLIQPPAGVDVGMVAPKGPGHLVRRTYTEGGGVPALIAVSQDPSGK
                     TRDLALSYAAAIGAARAGVLETTFDEETETDLFGEQVVLCGGLTSLIMGGFETLVEAG
                     YQPESAYFECLHEVKLIVDLMYEQGIAGMRFSISDTAEYGDLTRGPRVIDDHVRAEMR
                     RILAEIRDGSFAAEWVAENRAGRPIFNQLRAAGAEHPIEKVGAELRAMMPWISAGRER
                     PQDVSGG"
     gene            complement(3951..4364)
                     /locus_tag="VF954_02810"
     CDS             complement(3951..4364)
                     /locus_tag="VF954_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9530040.1"
                     /translation="MASNAPQPRVLVADDDPDIRRILAVNLEAMGYQVTEVSDGEEAL
                     RLARQMVPAFVVLDVMMPELDGYGVLHSLRGHPVTARIPVVMLSALTTDRDIWDGWQA
                     GADYYLTKPFMLDELLHFVELLELGRPPEGAVLAP"
     gene            complement(4369..>5993)
                     /locus_tag="VF954_02815"
     CDS             complement(4369..>5993)
                     /locus_tag="VF954_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9530041.1"
                     /translation="KIVFNSAQAATASALAATVFRLAGRPAATEPHAWLAVFAAAGVY
                     FLVNVTSVCAAIAISEDRPFAQLLRTTLAPAAVATTGSTSLGVLVAVLAPQGSVVALV
                     VPVLGLLMVSSRSVAVHRAERLRFERLYAASFRTGRLLGVDDALVTLAGEARALVTGS
                     AALCAVDHPDGTCSAVVVDDTGNRPATTDELAAARALLADRAEDQHAVTLSRHQVPAQ
                     LQWVDRDVVEAVAAISPPGDDDRVLMVVLRSLDDARDHGHGDVLAVFCRHAALTVANA
                     RLYQEVEAALHRQVDLNRQKDDFLAAVSHELRTPLTSILGSVDTIARLGERMSEDRRA
                     QFCAMAKNQGLRLKRLIEELLLVAAADAGSMGCAVECIDVPRLMSGLALEFGPLSNDR
                     VVVSATGGDAPLFSDEDKVRRVLVNLIENAVKYAPAGLIEVEAERMGSHWQFEVRDHG
                     PGISIRDRERIFERFVQLDQSSTRRHGGTGLGLYLCRQLAEALGGSLTVSGVNPGGTR
                     FVLTVPSLVSDSGAAEYLAVSTRHAISSADIT"
BASE COUNT          930 a         2061 c         2160 g          842 t
ORIGIN      
        1 ctcgtgacga tggcgacgcc ggggcggccg gtgacatgtg cgtacccctc ggccatgtga
       61 ccggcgcgga gcggggagcg gtcaccgagg cctgatcgcc tcgcggtcgc cctttctccc
      121 gacttctccc gacggtcagg agttgccggc cccgtcggtc tggctctgct gctggttgag
      181 ctgggtgacg acggtcttgg ccctgttgat cgggtcggtg atcacgccgt gggcgttggg
      241 gtcgacattg gcgcccggcg ctgggggctg ggtggtggcg gtcggcgccg aggccggtcc
      301 gctcccgccc gagcaggcgg ccaggccggc ggagagtacg gcggcgagca gcagctggcg
      361 cgcgactttc atgtcgaggt tgtcggcgcg agagaccgag gcctggagcg ctgttgccaa
      421 gcggctcggg ctctgccaga ctgaacggcg atgactcgcc gccccgcgct cgtactcatc
      481 acctagcgca cgccgggtcc atcgcgtgcg cctcacgacc tcccagccgg gaggtcgttt
      541 tcattccgcc gggagttgcc caccatgaag ctgaccgggg cccaagccct cataaagagc
      601 ctggagatgg agcagaccga ggtcgtcttc ggactgcccg gaggcgccat cctcccggcc
      661 tacgacccca tcatcgactc gcccatccgc cacatcctgg tccgtcacga gcagggcgcc
      721 ggtcacatgg ccgaggggta cgcacatgtc accggccgcc ccggcgtcgc catcgtcacg
      781 agcgggccgg cggccaccaa catcgtcacg ccgctgtgcg acgcccacat ggactcggtg
      841 ccgatcgtgg tcgtcaccgg ccaggtcggc cgccccatgc tcggcacgga cggcttccag
      901 gagtgcgaca ccgtcggcat cacccgctcg gtgaccaagc acaacgagct ggtgatggac
      961 cccgacgaca tcccccggat catgcgcgag gcgttccaca tcgccacgac cgggcggccg
     1021 gggccggtgc tggtcgacat cccgaaggac gtcctgtcct ccaccatgga gtggtactgg
     1081 cccacgtcgg tcgacctgcc cgggtaccgg ccgaccacca ggggccatcc ccggatgatc
     1141 aaggaggcgg cccgggtcat ctgcgagtcc cgccgcccgg tgatctacgc cgggggcggc
     1201 atcctcaagg cgagggcgac cgacgccctc aaggagctgg ccgagctgac cggcatccct
     1261 gtcgtgacca ccctcatggc caggggggcc ttcccggaca gccacgagct gtgcctcggc
     1321 atgccgggga tgcacggcaa ctacaccgcc gtcacgtcca tgcagcgggc cgatctgctg
     1381 atcgccctcg ggtcgcgctt cgacgaccgg gtgacgggga aggtgagcgg gttcgcgccc
     1441 gacgcccagg tcatccacgt cgacatcgac ccggccgagc tgggaaagat ccgacgccct
     1501 gacgtcccca tcgtcggcga ctgccggctg gtgatcgagg agctggtcaa ggccatcgca
     1561 gccataggcg gtagcggggc ccagcccgac cgttcgccgt ggatggcgca gatccggcag
     1621 tggcagcgcg acttcccgct gcactacgcc caggactggg acggcaacgg gccgctcaag
     1681 ccccagtacg tgctcgagca gctccgggac aacacgcccg acgacaccat cgtcgtgtcc
     1741 ggcgtcgggc agcaccagat gtgggcctcg cagtactggc gcttcgacca tccctacacc
     1801 tgggtcaact cgggcggcct cggcaccatg ggcttcgccg tcccggccgc ggtgggcgcc
     1861 aaggtgggcc ggcccgaccg tatggtctgg gccgtcgacg gcgacgggtg cttccagatg
     1921 acggcccagg agctggtgac ggcgtcgtcc gagcggatcc ccatcaaggt ggccatcctg
     1981 aacaacgcct acctgggcat ggtccggcag tggcaggaga tgttctacga ggagcgctac
     2041 tccgaggtgt acctgtcgcc cgacctgccc gactacgtca aatgggccga ggccatgggc
     2101 tgtgccggct tccgggtcga cactcccgaa gacgtcctgc ccaccatcga gaaggccaac
     2161 gacatcgacg accggcccgt cgtgatcgac ttccggaccg actccatgga gaaggtgtac
     2221 ccgatggtcg cggccggggc caccaacgac gagatcgtcg tcgaccctgc ccatgggcag
     2281 ggggggcgtt gatgccgcag accacggggc accacatcct gtcggtcctc gtcgagaaca
     2341 aggccggcgt gctggcccgg gtggccagcc tgttctcccg gcggggctac aacatctact
     2401 ccctggcggt ggccccgacc gacgacgagc ggttcagccg gatcaccatc gtcgtcgatg
     2461 ccgagtcggc gcccctcgag cagatcacca agcagctgtt caagctcatc aacgtgatca
     2521 agatctcaga gctcgatccg gccgaggcgg tggagcgcga gctgctcatg gccaccgtga
     2581 gggccgagcc cgggacgcgg ggccaggtga tcgagctggt gcaggtgttc gagggcaaga
     2641 tcgtcgacgt cggccaggac gagctgacgg tgatgctggc cgggacgccg gccaagctcg
     2701 acgacttcga ggacctcatg cgaccctatg ggatcaacga gctgcagcgc accggccggg
     2761 tcgcgctgcc gagactggag cggcaggcgc cccgcctccg cagcgtgacc gggaaggtga
     2821 gctaacccat ggccagcatc tactacgaca aggacgccga tccggccctc atcgccggtc
     2881 gcaaggtcgc catcctgggc tacggctccc agggccacgc ccacgccttg aacctccgcg
     2941 actcgggggt ggacgtgcgc gtagggctgc gccaagggtc cgcatcggcg gccaaggcgt
     3001 cgtccgctgg cctgcgcgtc ctgcccaccg accaggccgc ggccgaggcc gacctgatca
     3061 tgatgctgct ccccgacacg gagcaggcgg cggtctacac cgacgccgtc gcgccccgcc
     3121 tcgcccctgg cgacgccgtg ttcttcgctc acgggttcaa catccgtttc gggctcatcc
     3181 agcctccggc cggcgtggac gtcggcatgg tggcgccgaa ggggcccggg cacctggtcc
     3241 ggcggaccta caccgagggc ggcggcgtgc ccgccctcat cgccgtctcc caggacccgt
     3301 cgggcaagac gcgcgacctc gccctgtcct atgcggccgc catcggcgcg gcccgggccg
     3361 gcgtgctcga gacgaccttc gacgaggaga ccgagaccga cctcttcggt gagcaggtgg
     3421 tgctctgcgg gggcctgacc tcgctgatca tggggggctt cgagaccctg gtggaggccg
     3481 gctaccagcc ggagtcggcg tacttcgagt gcctccacga ggtgaagctg atagtggacc
     3541 tcatgtacga gcagggcatc gccgggatgc ggttctccat ctcggacacc gccgagtacg
     3601 gcgacctcac cagggggcca cgggtgatcg acgatcacgt ccgggccgag atgcgccgca
     3661 tcctggcgga gatccgcgac gggtcctttg cagccgaatg ggtggccgag aaccgggcgg
     3721 gacggccgat cttcaaccag ctgcgggccg cgggggccga gcatcccatc gagaaggtgg
     3781 gcgccgagct gcgggcgatg atgccgtgga tctccgccgg ccgggagcgc cctcaagacg
     3841 tgtcaggggg ctaagaggat gcgccgacag gcgtagcctc ggccggctgg gcagggaccc
     3901 ggccggcaca gcgatgagcc cttcagggcg aagagccatc gagcaaggtg tcagggggcc
     3961 agcacggccc cctcgggtgg gcgcccgagc tcgaggagct cgacgaaatg caggagctcg
     4021 tcgagcatga agggcttggt caggtagtag tcggccccgg cctgccatcc gtcccagatg
     4081 tcgcggtcgg tggtcagcgc ggacagcatc accaccggta tgcgcgccgt cacgggatgg
     4141 ccccggagag agtggagcac gccgtagccg tccagctcgg gcatcatcac gtccagcacc
     4201 acgaaggccg gcaccatctg gcgggccagc cggagggcct cctcgccgtc ggagacctcg
     4261 gtgacctgat agcccatggc ttcgaggttg acggcgagga tgcggcggat gtcggggtcg
     4321 tcgtcggcga cgagcacgcg gggctgggga gcgttggagg ccatgttcct aggtgatgtc
     4381 ggcggacgag atggcgtggc gggtcgacac ggccaggtac tcggccgcgc cggagtcgga
     4441 gaccaggctg gggacggtga gcacgaaccg ggtgccgccg gggttgacac ccgagacggt
     4501 gagggagccg ccgagcgcct cggccagctg ccggcacagg tacaggccca ggccggtgcc
     4561 gccgtggcgg cgcgtcgacg actggtcgag ctgcacgaag cgttcgaaga tccgctcccg
     4621 gtcccggatc gagatgcccg gaccgtggtc tctgacctcg aactgccagt gggagcccat
     4681 cctctcggct tccacttcga tgaggccggc cggagcgtac ttgaccgcgt tctcgatgag
     4741 gttgacgagc acccgccgca ccttgtcctc gtcgctgaag agcggggcgt cgccgcccgt
     4801 cgccgagacc accacccggt cgttggacag cgggccaaac tccagggcga ggccgctcat
     4861 cagccggggg acgtcgatgc actccacggc gcagcccatg gacccggcgt cggctgcggc
     4921 caccaggagc agctcctcga tgaggcgctt gaggcgcagg ccctggttct tcgccatggc
     4981 gcagaactgc gctcgcctgt cctcggacat gcgctcaccg aggcgcgcga tggtgtcgac
     5041 cgagccgagg atggacgtca gcggcgtgcg cagctcgtgg gagaccgcgg cgaggaagtc
     5101 gtccttctgg cggttcaggt ccacctgacg gtgcagggcc gcctccacct cctggtagag
     5161 ccgggcgttg gccacggtca gggcggcgtg ccggcagaac acggccagca cgtcgccgtg
     5221 gccgtggtcg cgtgcgtcgt ccaggctccg gagcaccacc atgaggacgc gatcgtcgtc
     5281 gcccggcggc gagatggcgg ccaccgcctc gaccacgtcg cgatccaccc actggagctg
     5341 ggcggggacc tgatggcgcg acagcgtcac cgcgtgctgg tcctcggcgc gatcggcgag
     5401 cagggcccga gccgcggcca gctcgtcggt ggtggccgga cggttgcccg tgtcgtcgac
     5461 caccaccgca gagcacgtgc catcggggtg gtccaccgcg cacagggcgg cgctgcccgt
     5521 gacgagggcc cgggcctccc cggccagggt gacgagggcg tcgtcgacgc ccagcagccg
     5581 gccggtgcgg aaggacgcgg cgtacaggcg ctcgaagcgg agccgttcgg cgcggtgaac
     5641 ggcaaccgac cggctggaca ccatgagcag gccgaggacc gggaccacca gggcgacgac
     5701 ggagccctgg ggggccagca cggccacgag cacgccgagc gacgtggacc cggtcgtggc
     5761 taccgccgcc ggtgccaggg tggtccgcag cagctgggcg aagggccggt cctcggagat
     5821 ggcgatggcg gcgcacaccg aggtcacgtt gaccaggaag tagacgccgg cggccgcgaa
     5881 cacggccagc caggcgtgcg gttcggtcgc cgccggccgg cccgccagcc tgaacacggt
     5941 ggcggcgagg gcgctggcgg tggccgcctg ggccgagttg aacacgatct tgg
//
LOCUS       DASWGG010000156         3965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2330520, whole genome shotgun sequence.
ACCESSION   DASWGG010000156 DASWGG010000000
VERSION     DASWGG010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2330520"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VF954_02820"
     CDS             complement(<1..1102)
                     /locus_tag="VF954_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9530042.1"
                     /translation="MRVRAAATAMCLIAGGIAGGAAASGAAAVAAGPSPSGPVVVVAA
                     DEAAAARAVAGAGASVDVALPLSNAVAARVTPAQSARLEAEGLTVVADRSLRVNSTDF
                     TPASGDTQLAATDPGSHWSLGSGTGVSVALIDTGVDPSPDLGARLVQGPDLSGEGTGL
                     DSYGHGTFMAGLIAGDGTASADLATRHVGLAPSARIVSVKVAGADGTTSLSRVLEGIG
                     WAVVHRDDLHIGVMNLSFGVAPLGPTVLDPLAGAVEAAWASGITVVASAGNGGPGQVT
                     MPGADPWILTVGAADTHGTATTADDTLAPWSGQAVTSDGTPKPEVVAPGMHTVSLRAV
                     GSTIDVQNPGARVDGAYFVGSGTSMATAIAAGA"
     gene            complement(1345..2580)
                     /locus_tag="VF954_02825"
     CDS             complement(1345..2580)
                     /locus_tag="VF954_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530043.1"
                     /translation="MPHDHQPPALAWIIPRNRSITRFFGALVAMGAAWLVPTLPLAAR
                     TSRAPTSAELARTSTRSPVPARRPEPIGPTTTAAPVPPKAWILVDADTGKVIDGTDVH
                     TPMRPASLTKILTALAVVESVPPDATIPVSARAEGMPAAKINMKAGQVWSFDDAFHSM
                     LLSSANDAAVALAERADGNLDAFSQHLRQVAAEIGLQDNPTLNDPAGLDDAEFSFGGG
                     NLLSARDLAIATRSALASAEVASAVSDPVYSFVGPDGAHHKLGNHNKLLKLYPGAIGV
                     KTGYTRQAGNTYIGAARRDGRTMIVVLLGASDVYATTSAYLDKGFATPVSAEPSTDQL
                     PGLHTVAFHGQDAGPRTADPPAELGRPATVLTAHHQPVWATPPALALELLTATVGVLR
                     LRARRRRRRRVGRRAPASG"
     gene            complement(2643..3776)
                     /gene="serC"
                     /locus_tag="VF954_02830"
     CDS             complement(2643..3776)
                     /gene="serC"
                     /locus_tag="VF954_02830"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX9530044.1"
                     /translation="MASARIPDIRIPPDLLPADGRFGCGPSKVRTDALAALGATGRAY
                     MGTSHRQAPVRSMVGRMRTGLRDLFSLPDGYEVLVGDGGTTAFWDAATFGLIAVRSQH
                     LSFGEFSSKFANAAAAAPHLEAPQVIVAPAGTRPEPEADPAVDLYAFTHNETSTGVMM
                     DIRRLAGADGLVAVDATSAAGGLRVEPDEFDVYYFAPQKCFASDGGLWIALCSPAAVE
                     RITAIKASGRWIPAFFDLTVALDNSRKDQTYNTPSLATVFLFVDQLEWMLANGGLDWA
                     AGRCDRSAERLYGWAEASPFATPFVSKPDERSHVVGTVDFDASVDAAAVAGALRANGV
                     VDTEPYRSLGRNQLRVGMFPAVDPEDVAALTQCIDYVMEALAD"
BASE COUNT          521 a         1322 c         1573 g          549 t
ORIGIN      
        1 ctgcgcctgc ggcgatcgcc gtggccatgg acgtgccgct gcccacgaag taggcgccgt
       61 cgacccgggc gccggggttc tgcacgtcga tggtcgatcc cacggcccgc agcgacacgg
      121 tgtgcatgcc gggggcgacg acctccggct tcggggtgcc gtcggaggtc acggcctggc
      181 cggaccacgg ggccagggtg tcgtccgcgg tggtggcggt gccgtgggtg tccgcggcgc
      241 cgaccgtcag gatccacggg tcggcacccg gcatggtgac ctggcccggg ccgccgttgc
      301 cggccgaggc gaccaccgtg atgccggacg cccacgcggc ttccaccgcg ccggccaggg
      361 ggtcgaggac cgtcgggccg aggggggcga cgccgaagga caggttcatc acgccgatgt
      421 ggaggtcgtc gcggtggacc acggcccagc cgatgccctc gagcacccgc gacagagagg
      481 tggtgccgtc ggccccggcc accttgacgg agacgatgcg ggcgctcggc gccaggccga
      541 cgtggcgggt cgccagatcg gcgctggccg tgccgtcgcc tgcgatcagg ccggccatga
      601 acgtgccgtg cccgtagctg tccagacctg tgccctcgcc cgacaggtcc ggcccctgca
      661 ccagccgggc gccgaggtcg ggggaggggt cgacgccggt gtcgatcagg gccaccgata
      721 cgccggtgcc ggacccgagg gaccagtggg acccgggatc ggtggcggcc agctgcgtgt
      781 cgcccgacgc aggggtgaag tcggtggagt tgacccgaag agagcggtcg gccacgaccg
      841 tcagcccttc ggcttcgagg cgcgccgact gggccggcgt gacgcgcgcc gccacggcgt
      901 tgctcagggg gagggcgacg tcgaccgagg caccggcccc ggcgacggcg cgggccgcgg
      961 ccgcctcgtc ggcggccacc acgaccaccg ggccggaggg cgaggggccg gccgcgaccg
     1021 cggctgcgcc gctcgcggcg gcaccccctg cgatgccccc ggcgatgagg cacattgccg
     1081 ttgctgccgc ccgaacccgc atgagccgtc ccatcctcgt ggaaaccact gtccgtggta
     1141 ttcggtgtga cggagggtgg cggttgagcg ggagtcggta cctatttccg aaatggcctg
     1201 gccgggtcag gtacggggtg gcacgagcgg tctagtaccg ggtcacccgc aggcgggggc
     1261 tggtctagta cggaggggtc acccgctggc gggagctggt ctagtaccag ctcacccgat
     1321 ggcgggggct ggtctagtgc cgggtcaccc gctggcgggg gcccgccggc cgacccggcg
     1381 cctccgccgc cggcgggcgc gcaggcgcag cacgccgacc gtcgccgtca gcagctcgag
     1441 ggccagcgcc ggcggggtgg cccagacggg ctggtggtgg gccgtgagca cggtggccgg
     1501 ccggccgagt tcggccgggg gatcggcggt gcggggaccg gcgtcctgac cgtgaaaggc
     1561 gacggtgtga aggccgggca gctggtcggt ggatggctcg gcggagacgg gggtggcgaa
     1621 ccctttgtcc aggtaggcgc tggtcgtggc gtagacgtcg ctcgcgccca gcaggaccac
     1681 gatcatggtg cggccgtcgc gccgggctgc gccgatgtag gtgttgccgg cctgcctggt
     1741 gtagcccgtc ttgaccccga tggcccccgg gtacagcttg agcagcttgt tgtggttgcc
     1801 cagcttgtgg tgggcgccgt cgggtccgac gaaggagtag accgggtcgc tgacggcgga
     1861 ggcgacctcg gcgctggcga gcgccgatcg cgtcgcgatg gccaggtcac gggcactgag
     1921 caggttcccc ccgccaaagg agaactccgc gtcgtcgagc ccggccgggt cgttgagcgt
     1981 cgggttgtcc tggaggccga tctcggcggc gacctggcgc aggtgctgcg agaacgcgtc
     2041 gaggttgcca tcggcgcgct cggccagggc cacggcagcg tcgttggcgg acgagagcag
     2101 catggagtgg aaggcgtcgt cgaagctcca gacctggccg gctttcatgt tgatcttggc
     2161 ggccggcatg ccctcggcgc gggcgctgac cgggatggtg gcgtccggcg ggacggactc
     2221 caccacggcc agggcggtga ggatcttggt caggctggcg ggccgcatag gtgtgtggac
     2281 gtcggtgccg tcgatgacct tgccggtgtc ggcgtccacc aggatccacg ccttgggcgg
     2341 gaccggggcg gcggtcgtgg tcggcccgat gggttcgggc cggcgcgccg gaaccggcga
     2401 ccgcgtcgag gtgcgggcga gctcggctga tgtcggtgcc ctcgacgtcc gggccgccag
     2461 gggcagggtc ggcacgagcc acgccgcgcc catggccacg agcgcgccga agaagcgggt
     2521 gatcgaccgg ttccggggga tgatccaggc tagggcaggc ggctggtggt cgtggggcat
     2581 cgtggtgggg ggaccgcggg ctcatggggc atccgccggc tggtggggca tccgccggct
     2641 ggtcagtcgg cgagggcctc catcacgtag tcgatgcact gggtgagggc ggccacgtcc
     2701 tccgggtcca cggccgggaa catgcccacc ctcagctggt tccgccccag gctccggtac
     2761 ggctcggtgt cgaccacacc gttggcgcgc agcgctccgg cgacggccgc cgcgtcgacg
     2821 gacgcgtcga agtcgaccgt gccgaccacg tggctgcgct cgtccggttt ggacacgaac
     2881 ggggtggcga aaggagacgc ctcggcccat ccgtagagcc gctcggctga ccggtcgcag
     2941 cggccggccg cccagtcgag ccctccgttc gcgagcatcc actcgagctg gtccacgaac
     3001 aggaacacgg tcgccaggct gggcgtgttg taggtctggt ccttgcggct gttgtcgagg
     3061 gcgacggtga ggtcgaagaa cgcgggtatc caccggccgc tggccttgat ggcggtgatg
     3121 cgctcgaccg cggcggggga gcagagcgcg atccacagcc cgccgtcgga ggcgaagcat
     3181 ttctgcggcg cgaagtagta cacgtcgaac tcgtcgggct ccacccgcag cccgcccgcg
     3241 gcagacgttg cgtccacggc caccagcccg tcggcgccgg ccagccggcg gatgtccatc
     3301 atcacgccgg tggaggtctc gttgtgcgtg aaggcgtaca ggtcgacggc gggatccgcc
     3361 tcgggctcgg gccgcgtgcc cgccggtgcc acgatgacct gaggagcctc gaggtgtggc
     3421 gccgcggcgg ccgcgttggc gaacttggac gagaactccc cgaacgacag gtgctggctt
     3481 cggaccgcta tcagcccgaa ggtggcggcg tcccagaacg ccgtcgtgcc gccgtctccg
     3541 acgaggacct cgtacccgtc gggcagggag aacagatccc gcaggccggt ccgcatccgg
     3601 ccgaccatgg accggaccgg ggcctgacgg tggctcgtgc ccatgtaggc gcggccggtc
     3661 gcgcccaggg cggccagggc gtcggtcctg accttggacg gtccgcagcc gaagcggccg
     3721 tcggcgggga gcagatcggg cgggatgcga atatcgggga tgcgcgcgct ggccactgtg
     3781 ccagcataag agccatggcc cccacccccg ctccctccag agtgtcgagg cgcatagccg
     3841 ccatcgccga gtcggcgacg ctcgccgtgg atgccaggta caaggcgctc caggcggccg
     3901 gagaggacgt gatcggcttc gggtcgggcg agcccgactt cccgacgccc accgccatcg
     3961 tggag
//
LOCUS       DASWGG010000157         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_395957, whole genome shotgun sequence.
ACCESSION   DASWGG010000157 DASWGG010000000
VERSION     DASWGG010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395957"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VF954_02835"
     CDS             <1..576
                     /locus_tag="VF954_02835"
                     /inference="COORDINATES: protein motif:HMM:NF025744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4417 domain-containing protein"
                     /protein_id="HEX9530045.1"
                     /translation="VWTKKASLLPELARQRWDLVLAPNYSMYGSQPRAEHLLNFRRNL
                     LVAAELEDLGVPAVPNIYWFRREDLDRYLVWMADVTPAAIAVNLQTFRTGADWEQMAL
                     PGLTYLSLGMPDKTKLVATGTSRADRIADLGGLFPGRLILVSQNPLQYARHGAVMTPA
                     GRVDRQARVEDLFAANVWHYARVVSQAGGAC"
     gene            570..884
                     /locus_tag="VF954_02840"
     CDS             570..884
                     /locus_tag="VF954_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530046.1"
                     /translation="MLRRVFVLLAHDPATGLATRPVGAIGLEDGRHYASLLAHVPAGE
                     AWRERLALVDVPLAEAVEVWLDAAGGVALDLAEVPAPASPDLAGAVEIVVDELLAMAG
                     VD"
     gene            884..>1981
                     /locus_tag="VF954_02845"
     CDS             884..>1981
                     /locus_tag="VF954_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530047.1"
                     /translation="MGSGAKSKHASYPVAPAESATAVAPSVPASDTQAVTAAGEAYTE
                     AVAQLDGVQDFEGLTQATAAVLEAQSSLVDEGWPPQLAAEALKGLKAQTANYLGNLSI
                     EELQELAAAQGFEHPSLVGWNTPPGGQHPLVHWLDPVYPAESPSKLAIQAKAAERYAQ
                     LAAGQTLNGLSLADVAAQEAGFPGHNQPGSWQATPADVVGAMTSFNQALAEAPPLKAN
                     GEQLVALIAAENHLSASVCPGMAAEQLEAAKAAAKANVDQRLASASWAPRQEAASKLV
                     TEVSAQGAVDPATAKYLSAADQLALVRRSTAAGEREGLQGLASERAGQVATLKDLHQA
                     YTAHGLASLDPKAASPAQVAEWAKTTGAYVAT"
BASE COUNT          293 a          737 c          691 g          260 t
ORIGIN      
        1 gtctggacga aaaaggcctc gcttctcccg gagctcgccc gccagcgctg ggacctggtc
       61 ctggcgccca actactcgat gtacgggtcc cagccccgcg ccgagcacct cctcaacttc
      121 cgtcgcaacc tcctggtcgc cgccgagctc gaggacttgg gggtcccggc ggtgcccaac
      181 atctactggt tccgcaggga ggacctcgac cgctacctcg tctggatggc cgacgtgacg
      241 ccggcggcga tcgccgtaaa cctccagacc ttccgcaccg gggcggactg ggagcagatg
      301 gcgcttccgg ggctcaccta cctgtcgctc gggatgcccg acaagacgaa gctggtggcg
      361 accgggacct cgagggccga ccgcatcgcc gacctgggcg gactgttccc cggccgcctc
      421 atccttgtca gccagaaccc cttgcagtac gcacgccacg gggccgtgat gacccccgcc
      481 ggtcgggtgg accgccaggc ccgggtcgag gacctgttcg ccgcgaacgt gtggcactac
      541 gcccgggtcg tgagccaggc ggggggtgcg tgctgaggcg cgtcttcgtc ctcctcgccc
      601 acgatccggc caccggactc gccacgcgcc ccgtgggcgc catcgggttg gaggatggtc
      661 gccactacgc ctcgctgctg gcccacgttc cggcggggga ggcgtggcgg gagcgcctgg
      721 ccctcgtcga cgtgcccctc gccgaggcgg tggaggtgtg gctggacgcc gccggcggag
      781 tggccctgga cctcgccgag gtgccggcgc cggcgtcccc ggacctggcc ggcgccgtgg
      841 agatcgtggt cgacgagttg ctcgccatgg cgggagtcga ctgatgggca gcggggccaa
      901 gtccaaacac gcctcctatc cggtggcccc ggccgagtcg gcgaccgccg tagcgccgtc
      961 ggtcccggct tccgacaccc aggcggtgac cgccgccggc gaggcataca ccgaggccgt
     1021 ggcacagctc gacggggtgc aggacttcga aggcctcacc caggcgactg cagctgtgct
     1081 cgaggcgcaa tcgtcgctgg tcgacgaggg ctggccgccg cagctggcgg ccgaggccct
     1141 caagggcctg aaggcgcaga cggccaacta cctgggcaac ctgtcgatcg aggagctcca
     1201 ggagctggcg gccgcccagg gcttcgagca ccccagcctg gtgggctgga acacgccacc
     1261 tggtggccag cacccgctcg tgcactggct cgaccccgtc taccccgcgg agtcgccctc
     1321 caagctcgcc atccaggcca aggccgccga gcgctatgcg cagctcgccg ccggccagac
     1381 cctcaacggg ctcagcctgg cggacgttgc cgcgcaggag gctggcttcc ctgggcacaa
     1441 ccagccgggc tcctggcagg ccacccccgc cgacgtggtc ggggccatga cctccttcaa
     1501 ccaggccctg gcggaggcgc caccgctgaa ggcgaacggc gagcagctgg tggcgctcat
     1561 tgctgccgag aaccacctca gtgcatcggt ctgccccggg atggcggcgg agcaactcga
     1621 ggcggcgaag gcggcggcca aggccaacgt cgaccagcgc ctcgcctccg ccagctgggc
     1681 gccgaggcaa gaggcggcgt cgaagcttgt cacagaagtc tccgcccagg gcgccgtcga
     1741 ccccgccacg gccaagtacc tcagcgccgc cgaccagctt gccctggtcc ggcgctcgac
     1801 ggctgcggga gagcgtgagg gcctccaggg cctggccagc gagcgcgccg gccaggtggc
     1861 cacgctcaaa gacctccacc aggcgtacac cgcccacggg ttggcgagcc tcgacccgaa
     1921 agcagcctcc ccggctcagg tggccgaatg ggccaagacc acgggggcat acgtcgccac
     1981 c
//
LOCUS       DASWGG010000158         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2330644, whole genome shotgun sequence.
ACCESSION   DASWGG010000158 DASWGG010000000
VERSION     DASWGG010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2330644"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /gene="nthB"
                     /locus_tag="VF954_02850"
     CDS             <1..673
                     /gene="nthB"
                     /locus_tag="VF954_02850"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR03888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HEX9530048.1"
                     /translation="AGVDGIHDMGGMQDWGRVHYDPDEPAFKAPWEGRAFAMSLLSMN
                     RLTGRNLDAMRHALERLTPLEYLKNGYYGRWLLLSETLLTDSGVIPPGAVDARARTLR
                     GDDVTEPPDVELSKPDYTATGPGSLRQIDDPPRFKVGQQVRAKDMHPTGHTRLPRYVR
                     GRIGTVEHIQPASVLPDTNAHFLGENAQHVYGVAFDSKDLWGEDAEPFVMHVDLFDSY
                     LEPAP"
     gene            670..1266
                     /gene="nthA"
                     /locus_tag="VF954_02855"
     CDS             670..1266
                     /gene="nthA"
                     /locus_tag="VF954_02855"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR01323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX9530049.1"
                     /translation="MSPVPAPSDLPVELRTEALESILVEKGLVDPAVLDKFIKTYAED
                     VGPLNGAKVVAKAWTNPEYKARLLADGTAAIAELGFKGPQGEHFVVLENTPTRHHLVV
                     CTLCSCYPWPVLGLPPGWYKDPAYRARAVREPRALLAEFGLELSPDVEIRVLDSVSEV
                     RYMVLPERPAGTESLSEEELVPLITREAMTGVAKVKAP"
     gene            1266..1625
                     /locus_tag="VF954_02860"
     CDS             1266..1625
                     /locus_tag="VF954_02860"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HEX9530050.1"
                     /translation="MALVDLDVEGPAAPPRSNGELVFAAPWEGRAFAMALTLHDRGFF
                     EWEAFQRQLIQAVARAEAADGEFSYYRCWLEALEGLLDSEGLVDVAAVEERAGVLACR
                     PAGHDHGHAHGDHDHDH"
BASE COUNT          252 a          613 c          564 g          232 t
ORIGIN      
        1 agccggggtg gacgggatcc acgacatggg cgggatgcag gattggggcc gtgtccacta
       61 cgaccccgac gagccggcct tcaaggcgcc gtgggaaggc cgggccttcg ccatgagcct
      121 gctgtcgatg aaccggctga caggccgcaa cctcgacgcc atgcgccacg ccctcgaacg
      181 gctcacgccg ctcgaatacc tgaagaacgg gtactacggc cggtggctcc tcctctcgga
      241 gacgctcctc accgacagcg gcgtgattcc gcccggggcc gtcgacgccc gggcccgcac
      301 gctccgcggc gacgacgtga ccgagccccc tgacgtcgag ctcagcaagc cggactacac
      361 cgccaccggt ccgggatcac tgcgtcagat cgacgacccg ccccgcttca aggtcggcca
      421 gcaggtccgg gccaaggaca tgcacccgac cggccacacc cggctgcccc gctacgtgcg
      481 ggggcggatc ggcaccgtgg agcacatcca gccggcgtcg gtgctgcccg acaccaacgc
      541 ccacttcctc ggcgagaacg cccagcacgt ctacggggtc gccttcgact ccaaggacct
      601 ctggggcgag gacgccgagc ccttcgtcat gcacgtcgac ctgttcgaca gctacctgga
      661 gcccgcacca tgagccctgt tcccgctccg tccgacctgc ccgtcgagct gcggaccgag
      721 gccctcgaat cgatcctggt cgagaagggc ctcgtcgacc cggccgtgct cgacaagttc
      781 atcaagacct atgccgaaga cgtcggcccc ctcaacggcg ccaaggtggt ggccaaggcc
      841 tggaccaacc ccgagtacaa ggcccgcctc ctggctgacg gcaccgccgc catcgccgag
      901 ttgggcttca agggcccgca gggcgagcac ttcgtcgtcc tggagaacac gccgacgcgg
      961 caccacctcg tggtctgcac gctgtgctcc tgctatccgt ggccggtgct cggcctcccg
     1021 ccggggtggt acaaggaccc ggcgtaccgg gcccgggcgg tgcgggagcc ccgggcgctg
     1081 ctggcggagt tcggtctcga gctgagcccc gacgtggaga tccgcgtcct cgactcggtg
     1141 tccgaggtcc gctacatggt gctgcccgag cggccggccg gcaccgagag cttgtcggag
     1201 gaggagctcg tcccgctcat cacccgggag gccatgaccg gcgtcgccaa ggtcaaggcg
     1261 ccctgatggc gctcgtggac ctcgacgtcg agggtccggc cgccccgccc cggagcaacg
     1321 gggagctggt cttcgccgct ccctgggagg ggcgggcctt tgccatggcg ctcaccctcc
     1381 acgaccgcgg gttcttcgag tgggaggcct tccaacggca gctgatccag gccgtcgccc
     1441 gggccgaggc ggccgacggc gagttctcct actaccgctg ctggctcgag gcgctggaag
     1501 gcctcctcga ctccgagggc ctggtggacg tcgcggcggt cgaggagcgg gccggcgtgc
     1561 tggcctgccg cccggccggt cacgaccatg ggcatgcgca cggcgaccac gaccacgacc
     1621 actgacgggt gcgcgacaag acgctgtcgg tcgagtcgac g
//
LOCUS       DASWGG010000159         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3058412, whole genome shotgun sequence.
ACCESSION   DASWGG010000159 DASWGG010000000
VERSION     DASWGG010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3058412"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VF954_02865"
     CDS             <1..410
                     /locus_tag="VF954_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX9530051.1"
                     /translation="SPIAGIARLPVPPGAASTAVSFALSHVGDPYVYGAAGPTQWDCS
                     GLTMVAYQKAGLIIPRVAQDQFDFGPRLPAGATLAPGDLLFFGSSPTAVGHVGMYVGN
                     GEMVDAPHTGAYVEVTSFAARTDFQGATRPTGL"
     gene            663..1844
                     /locus_tag="VF954_02870"
     CDS             663..1844
                     /locus_tag="VF954_02870"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9530052.1"
                     /translation="MLPTFADLGVAPALAAAVQDQGIEEPFPIQAMTIPDALAGRDVC
                     GKAKTGSGKTLAFGLPVLQRTPRAEPARPKALVLVPTRELALQVRDVLRPLGEVVDRR
                     VSALYGGAAMDRQIRALRRGIDVVIATPGRLIDLGDRGEVSVADVGTLVLDEADRMAD
                     MGFMPQVEWVLRRLPAVRQTLLFSATLDGDVDHLVKRYLTDPVRHEVESAQVTVAEME
                     HRFLQVHQLDKVKVAAAIARGAFRTLLFVRTKRGADRLVEQLNKEGVRAAAIHGDLRQ
                     PARERALRDFSAGKLPALVATDVAARGIHIEAIDVVIHYDPAEDHKAYLHRSGRTARA
                     GQAGVVATLLLWNQQLDVERLQKRLRLSEPIVEVFSNDPRLGDLITLNSPAPPRRAAT
                     I"
     gene            1938..>2218
                     /locus_tag="VF954_02875"
     CDS             1938..>2218
                     /locus_tag="VF954_02875"
                     /inference="COORDINATES: protein motif:HMM:NF039351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6112 family protein"
                     /protein_id="HEX9530053.1"
                     /translation="MVMVVVGEAVSLSPNVSSLPGAAALQQVTDGIGGWALVLALVGL
                     LIGAVMWALGSHGNNYQQTYTGRRAVLASGMAAVLIGAAPALVNFFFRIG"
BASE COUNT          326 a          784 c          773 g          335 t
ORIGIN      
        1 cgagccccat cgccggcatc gcccggttgc ccgtcccgcc tggagcggcg tcgacggccg
       61 tgtccttcgc cctgtcccac gtgggagatc cctacgtcta cggggcggcg ggccccacgc
      121 agtgggactg ctcgggcctg accatggtgg cctaccagaa ggccgggctg atcatcccca
      181 gggtggctca ggaccagttc gacttcgggc cccgcctccc ggccggcgcg acgcttgcgc
      241 ccggcgacct gttgttcttc gggtccagcc ccacggccgt cggccacgtc ggcatgtatg
      301 tcggcaacgg cgagatggtc gacgccccgc ataccggggc ctacgtcgag gtcacctcct
      361 tcgcagcccg gaccgacttc cagggcgcga cccggcccac ggggctctga cgcggcgcca
      421 tagggtcgaa cgcaaaacta cttgggcggt cgaagaccat ttccttgtag cgacgggcaa
      481 agttcccgag aaccgcccaa gtagttttgc gcttgaccct aacctgccct gacgaggttc
      541 ccccggtagc atgatcctgg cgagcagggt cacgaggacc cactcgcggg ccacctcgat
      601 cagctccacg tctcgcggca ggggttcgga ggccggccca ccaccgacgc acacgaggag
      661 caatgttgcc gaccttcgcc gatctcggcg tggcccccgc cctggccgcc gccgtccagg
      721 accaggggat cgaagagccc ttccccatcc aggccatgac catccccgac gcgctggccg
      781 ggcgcgacgt gtgcgggaag gccaagaccg gctctggcaa gacgctggcc ttcgggctcc
      841 ccgtgctgca gcgcacgccg cgggccgagc ccgcccggcc gaaggccctg gtgctggtcc
      901 cgacccggga gctggccctc caggtgcggg acgtgctgcg ccccctcggt gaggtcgtcg
      961 accgccgggt gagcgcactg tacggcggcg cggccatgga ccggcagatc cgcgccctgc
     1021 gccggggcat agacgtggtc atcgccactc caggccggct gatcgatctc ggcgaccggg
     1081 gcgaggtctc ggtcgccgac gtggggacgc tcgtgctgga cgaggccgat cggatggcag
     1141 acatgggctt catgccccag gtcgagtggg tcctgaggcg gttgcccgcc gtgcgccaga
     1201 cgctgctgtt ctcggccacc ctcgacggcg atgtcgacca tctcgtgaag cggtatctca
     1261 ccgatcccgt ccgccacgag gtcgagtcgg cccaggtgac cgtggccgag atggagcacc
     1321 gttttctcca ggtccatcag ctcgacaagg tgaaggtggc cgcggcaatc gctcggggcg
     1381 ccttccgaac gctgctgttc gtgcgcacca agcggggggc cgaccggctc gtcgagcagc
     1441 tcaacaagga gggtgtgcgg gcagccgcca tccacggcga cctgcgccag ccggcccggg
     1501 agcgggcgct gcgcgacttc tcggcgggca agctgccagc gctggtcgcc accgacgtgg
     1561 cggcgcgagg gatccacatc gaggccatcg acgtcgtcat ccactacgac cccgccgagg
     1621 accacaaggc gtacctccat cgctccggcc gcacggcgcg cgccggccag gcgggtgtgg
     1681 tcgcgacgct cctgctgtgg aaccaacagc tcgacgtcga gcgcctgcaa aagcgcctcc
     1741 gcctgagcga gcccatcgtg gaggtcttct ccaacgaccc gcgcctgggc gacctcatca
     1801 ccctcaactc cccggcgcct ccgcggcggg cggccaccat ttgagactgg gatataatga
     1861 aatagcttga aaggggcgaa ggtttaggcc atgctggtcc ctggccgcca gaggaggcgg
     1921 tcaggaggag gtgcgggatg gtcatggtcg tggtgggcga ggcggtgtcg ctcagcccca
     1981 acgtgtcgag cctgcccggg gccgcggcgc tccaacaggt gaccgacggg atcggcggct
     2041 gggccctggt gctcgccctg gtcggcctgc tcatcggagc ggtcatgtgg gctctcggct
     2101 cccacgggaa caactaccag cagacgtaca cgggccggcg ggcggtgctg gcgtcgggga
     2161 tggcggcggt gctgatcggg gcggcgcccg cgctggtcaa cttcttcttc cgcatagg
//
LOCUS       DASWGG010000160         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3059279, whole genome shotgun sequence.
ACCESSION   DASWGG010000160 DASWGG010000000
VERSION     DASWGG010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3059279"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1118)
                     /locus_tag="VF954_02880"
     CDS             complement(15..1118)
                     /locus_tag="VF954_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX9530054.1"
                     /translation="MTSMSTERMARARQHMASLGVDCLLLSLGADLPWLTGYEAMALE
                     RLTMLVLAADGDATLVVPRLEAPRVAEDPAAFSVLPWSEGADPVEIVATLVGARRNLA
                     ISDRAWSIFLLRLQAALPGGSWQPSSVVTAPLRAVKDAAEIAALRRASAAADRVAVQL
                     LAGDIPLIGRTEASVSADIGRRLLAEGHQRVNFAIVGSGPNSASPHHEPGARVIGPGE
                     AVVCDFGGALDGYCSDITRTVVTGPPTAELVDLYGVLEQAQAAAVRAAVVGTPCEEVD
                     GAARRPIVAAGYGDAFIHRTGHGIGLEEHEDPYLVAGNSEPLVAGHAFSVEPGIYLAG
                     RFGARIEDIVVATPSGPEALNRADHGLHVVDAT"
     gene            complement(1202..2842)
                     /gene="lipA"
                     /locus_tag="VF954_02885"
     CDS             complement(1202..2842)
                     /gene="lipA"
                     /locus_tag="VF954_02885"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX9530055.1"
                     /translation="MLRVRWLGRVPYRDAHALQRALFTSRGEDHLLLLEHPHVYTLGV
                     RADPDHVLVAPASVGAELVRTDRGGDVTYHGPGQLVGYPVVSVPMGPDAVPCYVHAVE
                     QLVIDVLADVGLPGASRLPGYPGVWVGAGTDAPRKICAIGVRVSRGRSMHGFALNVDP
                     DLGYFGHIVPCGIPDKGVTSLAAEGVAVGMAEVVDAVAGRAAERWAPGGSIERQDVAW
                     RVAPDELAPFTPGDGVGGTSVRLLGRLAAAGVDPDAGVPLAHRKPPWLRAPAHLGPGY
                     RALKATMRSLELVTVCEEAGCPNIFECWADGTATFMINGSRCTRACGFCLVDTQHPVP
                     VDPSEPERVALAVEQLGLAHAVVTAVARDDLADGGAAGFAATVEAIRRRCPDAAVEVL
                     IPDCKGDPDALDRIFAARPDVLNHNLETVARLQRAVRPSASYARSLAVLGRAAAAGLT
                     TKSGLVLGMGETEDEVVGALADLRAVGVGIVTLGQYLRPTARHLPVARWWAPPEFDRL
                     REVGEAMGFGHVQASPLTRSSYHARQAAGAARPAATVG"
     gene            complement(2843..>3335)
                     /locus_tag="VF954_02890"
     CDS             complement(2843..>3335)
                     /locus_tag="VF954_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX9530056.1"
                     /translation="EFPHVNASVGDDELIVHHDVHMGYAVDLNLEGLIVPVVHNADAK
                     RLRAIAREVADLAARARAKKLTADDIAGGTFTITNPGPFGTLVTAPIINQPQVAILST
                     DGVKKRPVVVTLADGTDAIAIHRVGNLAISFDHRAFDGAYASAFLAKVREILETRDWA
                     QEL"
BASE COUNT          460 a         1172 c         1317 g          386 t
ORIGIN      
        1 ggatcacccc gggctcaggt ggcgtcgacc acgtgcaggc cgtggtcggc tcggttgagc
       61 gcctccgggc ccgacggcgt ggcgaccacg atgtcctcga tccgggcgcc gaagcggccg
      121 gcgaggtaga tgcccggctc caccgagaag gcgtggccgg cgaccagggg ctcggagttg
      181 ccggccacga gataggggtc ctcgtgctcc tccaggccga tgccgtggcc ggtgcggtgg
      241 atgaaggcgt cgccgtagcc ggcggcgacg attggccggc gcgcagcccc gtccacctcc
      301 tcgcacggcg tgccgaccac cgcggcccgc acggcggcgg cctgggcctg ctccagcacg
      361 ccgtacagat cgaccagctc ggcggtgggc ggcccggtga cgaccgtgcg agtgatatcc
      421 gagcagtagc cgtcgagggc gccgccgaag tcgcagacga ccgcctctcc gggaccgatc
      481 acgcgggcgc cgggctcgtg gtggggcgag gccgagttgg ggccgctgcc cacgatggcg
      541 aagttgaccc gctggtgccc ctcggccagc agccgccggc cgatgtcggc ggacaccgac
      601 gcctcggtcc ggccgatgag cgggatgtca ccggcgagga gctggacggc gacccggtcg
      661 gcggcggcgg acgcccgccg cagggcggcg atctcggccg cgtccttgac cgcccgcaac
      721 ggcgcggtca ccaccgacga cggctgccac gacccgcccg gcagggcagc ctggagccgg
      781 agcaggaaga tcgaccaggc ccggtccgag atggccaggt tgcgccgggc gccgacgagg
      841 gtggccacga tctcgaccgg gtccgcgccc tcggaccagg gcagcaccga gaaggcggcc
      901 gggtcctcgg ccacgcgcgg cgcctccaag cggggcacga cgagggtggc gtcgccgtcg
      961 gcggccagca cgagcatggt cagccgctcg agcgccatgg cctcgtagcc cgtgagccag
     1021 ggcagatcgg cgccgagcga gaggagcagg cagtccaccc cgagggaagc catgtgctgg
     1081 cgggccctgg ccatccgctc ggtgctcatg gacgtcatcg gccggcagcc gagggcgcgg
     1141 gcgggcagga cgtcatcggc cggcagccga gggcgcgggc gggcggggcg tcatcagccg
     1201 ttcagccgac ggtggcggcg ggccgggcgg ccccggcggc ctggcgggcg tggtagctcg
     1261 aacgggtcag cggcgaggcc tgcacgtggc cgaaccccat ggcctcgccc acctcccgca
     1321 gccggtcgaa ctccggcggc gcccaccacc tcgccaccgg caggtgacgg gcggtgggcc
     1381 gcaggtactg cccgagggtc acgatgccca caccgacggc gcgcaggtca gcgagggcgc
     1441 cgaccacctc gtcctcggtc tcgcccatgc cgaggaccag gcccgacttg gtggtcagcc
     1501 cggcggccgc ggcccggccc agcacggcca ggctgcgggc ataggaggcc gacgggcgca
     1561 cggcccgctg gagccgggcc accgtctcga ggttgtggtt gagcacgtcg gggcgggccg
     1621 cgaagatccg gtcgagggca tcggggtcac ccttgcagtc ggggatgagc acctcgacgg
     1681 cggcatcggg gcaccgccgg cggatggcct cgacggtggc ggcgaagccg gccgcgccgc
     1741 cgtcggccag gtcgtcgcgg gcgaccgcgg tcacaaccgc atgagccagg cccagctgct
     1801 cgacggcgag ggccacccgc tcgggctccg acgggtcgac cggaacgggg tgctgcgtgt
     1861 cgaccaggca gaagccgcac gcccgggtgc agcgcgaccc gttgatcatg aaggtggcgg
     1921 tcccgtcggc ccagcactcg aagatgttgg ggcagccggc ctcctcgcac acggtgacca
     1981 gctcgagcga ccgcatggtg gccttgagcg cccggtagcc ggggccgaga tgggcgggag
     2041 cccggagcca ggggggcttg cggtgggcga gggggacgcc ggcgtccggg tccacgccgg
     2101 ccgcggccag ccggcccagg agccgcacgg acgtgccgcc cacgccatcg cccggcgtga
     2161 agggggccag ctcgtcgggc gcgacccgcc aggcgacgtc ctggcgctcg atcgagccgc
     2221 cgggcgccca gcgctcggcc gcccggccgg ccacggcgtc gaccacctcg gccatgccca
     2281 cggccacccc ttcagccgcc agggaggtga cccccttgtc gggtatgcca cacggcacga
     2341 tgtggccgaa gtacccgagg tcgggatcga cgttgagggc gaagccgtgc atcgagcggc
     2401 cccgcgacac cctgacgccg atggcgcaga tcttgcgggg cgcgtcggtg ccggcgccca
     2461 cccacacgcc ggggtagccg ggcaaccggg aggcgccggg cagcccgacg tcggcgagca
     2521 cgtcgatgac gagctgttcg acggcatgca cgtagcacgg cacggcgtcg gggcccatgg
     2581 gaacgctgac gacggggtag ccgacgagct ggccgggacc gtggtaggtg acgtcgccgc
     2641 cccgatcggt ccgcaccagc tcggcaccga ccgacgccgg tgcgaccagc acgtggtcgg
     2701 ggtcggcccg cacgccgagg gtgtagacgt gggggtgctc cagcaggagg aggtggtcct
     2761 cgccgcggga ggtgaacagc gctcgctgga gggcgtgggc gtcacggtac ggcacccgcc
     2821 ccagccagcg gacgcggagc acctacagct cctgggccca gtcgcgcgtc tcgaggatct
     2881 cccggacctt ggcgaggaac gccgacgcgt aggcgccgtc gaacgcgcgg tggtcgaagg
     2941 agatggccag gttccccacc cggtggatgg cgatggcgtc ggtgccgtcc gccagggtga
     3001 cgaccacggg gcgcttcttg acgccgtcgg tggacaggat ggcgacctgc ggctggttga
     3061 tgatcggcgc ggtgacgagc gtgccgaaag gtcccgggtt ggtgatcgtg aacgtcccgc
     3121 cggcgatgtc gtccgcggtc agcttcttgg cccgggcccg ggcggccagg tcggccacct
     3181 cgcgggcgat ggcgcgcagc cgcttggcgt cggcgttgtg caccaccggg acgatgaggc
     3241 cttcgaggtt gaggtccacg gcgtagccca tgtgcacgtc gtggtggacg atcagctcgt
     3301 cgtcgcccac ggacgcgttg acgtgcggga actcg
//
LOCUS       DASWGG010000161         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1444708, whole genome shotgun sequence.
ACCESSION   DASWGG010000161 DASWGG010000000
VERSION     DASWGG010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444708"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VF954_02895"
     CDS             complement(<1..943)
                     /locus_tag="VF954_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9530057.1"
                     /translation="MPDRDTGQHPLLTLPQAAEFLGLPVEAVQSLTRAGFLACDSSAN
                     YRPGDLKAFLARNADNGAGLDLAGDLDDADPQALLEALDGRSEEMARRAFDIFSTVFP
                     ETKEWTLKQRARFVEHAKGRFEAILAVTSQGAEVDEALVGDLEEVGAGAAWADSPLPQ
                     LLVVLRISRDLVVQTAVELAEQRGRHWGLALSLLLTRVLPAIDRLTDAIAQGYWAAIL
                     AREEEHRARYENVVEHSADGICEIDLEGRIQYANPSLALILGHTIEQLEGSTVGEVVV
                     AADRRPLPAELTSTSVDGGSRVELDIIRADGVRRTIEI"
     gene            1176..2156
                     /locus_tag="VF954_02900"
     CDS             1176..2156
                     /locus_tag="VF954_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX9530058.1"
                     /translation="MATVDDDADAAQPGPAAGAADTPAAPQPRQRWSERLIPRTVLGM
                     STVILAAAVGAAFSGTALYAYYEYRLTKTEHLVNSYVNGDFKKDYANAIDSLKSETAN
                     DKGQIQQQLGPLLKTQAAGATLAELVKQAQGSIWFVTTQDDSGQPSVGSAFVVASDGN
                     QSLMLTSFAVVQSSAKAPGPPEGISVHKGTDAEKATLWTWQPEKDLALLVVAKPNLPK
                     LNFGPADPKVGDRVYALSGLGGSGGAATQGLVADVSAAGIQHDAQIGAAFQGGPLLDG
                     SGNVVGLDSRAYAPLNFNPEAVFFAIPIRTACDKVLRCPSGGGPSAGSKG"
     gene            complement(2236..>2619)
                     /locus_tag="VF954_02905"
     CDS             complement(2236..>2619)
                     /locus_tag="VF954_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530059.1"
                     /translation="SMRNLRAFGQTYPLIFGGGIPAQTWHNFMSEALANVPSTDFNQP
                     PPIQKIVDAINKVARAGIDPGSRLYPGGTGSGGPYEIPAAPPVAVAPPTTTTSTTAPA
                     DQGGGGGGPGPPPPTTTTGLGVPGR"
BASE COUNT          388 a          926 c          934 g          371 t
ORIGIN      
        1 ggatctcgat cgtccggcgc accccgtcgg cccggatgat gtccagctcc acccgggacc
       61 cgccgtcgac cgaggtcgag gtcagctcgg ccgggagagg gcggcgatcg gcggccacca
      121 cgacctcgcc gacggtggac ccctcgagct gctcgatggt gtgaccgagg atcaaggcga
      181 gggacgggtt ggcgtactgg atgcggccct ccaggtcgat ctcgcagatc ccgtcggccg
      241 agtgctcgac gacgttctcg taccgggccc ggtgctcctc ctcccgggcc aggatggcgg
      301 cccagtaccc ctgggcgatg gcgtcggtca ggcggtcgat ggcgggcagc acgcgggtca
      361 gcaacaggga cagggccagg ccccagtgcc ggccccgctg ctcggccagc tcgaccgcgg
      421 tctggaccac caggtcgcgg ctgatgcgca ggacgaccag cagctggggc aggggtgagt
      481 cggcccacgc cgccccggcg ccgacctcct cgaggtcgcc gaccagggcc tcgtccacct
      541 ccgccccctg gctggtgacg gcgaggatgg cctcgaaacg gcccttggcg tgctcgacga
      601 acctcgcccg ctgcttgagc gtccactcct tggtctcggg gaacacggtg gagaagatgt
      661 cgaaggcccg gcgagccatc tcctcggacc tgccgtccag ggcctcgagg agggcctggg
      721 gatcggcgtc gtcgagatcg ccggccagat cgaggcccgc gccgttgtcg gcgttgcgcg
      781 ccaggaatgc cttcaggtcc cccggccggt agttggccga cgagtcgcac gcgaggaacc
      841 cggcccgggt aagggactgg acggcctcga ccggcaaccc caggaactcg gccgcctggg
      901 gaagcgtcag cagtggatgc tggccggtgt ctcggtcggg cattctcctt agttcgacct
      961 ttccgcctcc ggacctacct gcggggcggt ccctgcgcgc gccagaccgc cgcgtcgtca
     1021 cgaccacagg tcctcacgag cagatcctaa cggcccggcc cgctgacgtg gggctggcaa
     1081 ctggctacgc aattgcccgc tgcaaaaaga gacgccggcc ggcgtctcct tctccaggca
     1141 ttttgcgcgg ctcatcgggg ctaccctcgc aggcgatggc cacggtcgac gacgacgccg
     1201 acgcggcgca acccggaccg gcggccgggg cggccgatac cccggccgcg ccgcagccgc
     1261 gccaacgctg gtcggagcgc ctcatccccc gcaccgtgct cggcatgagc acggtgatcc
     1321 tcgccgccgc agtcggggcg gccttcagcg gaaccgccct gtacgcctac tacgagtacc
     1381 ggctgaccaa gaccgagcac ctcgtgaaca gctacgtgaa cggcgacttc aagaaggact
     1441 acgccaatgc catcgacagc ctgaagtccg agacggccaa cgacaagggc cagatccagc
     1501 agcagctcgg tcccctgctg aagacgcagg cggccggcgc cacgctcgcc gagctggtca
     1561 agcaggccca gggctcgatc tggttcgtga cgacgcagga cgactccggc cagccgtcgg
     1621 tgggctcggc gttcgtggtg gcctccgatg gcaaccagag cctgatgctg acgagctttg
     1681 ccgtggtgca gtcgtcggcc aaggcgccgg gcccacctga aggcatctcg gtccacaagg
     1741 gcaccgacgc ggagaaggcg acgctgtgga cctggcagcc ggagaaggac ctggccctgc
     1801 tcgtcgtggc caagccgaac ctgcccaagc tcaacttcgg cccggccgat ccgaaggtcg
     1861 gcgaccgggt gtacgccctg tccggcctcg gtggctcggg aggtgcggcc acccaggggc
     1921 tggtggccga cgtgtccgcc gcgggcatcc agcacgacgc ccagataggc gctgcgttcc
     1981 agggcggccc gctgctcgac gggtccggca acgtggtcgg gctggactcg cgcgcctacg
     2041 ccccgctgaa cttcaacccc gaggccgtct tcttcgccat cccgatccgc acggcgtgcg
     2101 acaaggtgct gcgctgcccg agcggcggcg ggccctcggc cggctccaag ggctgacccc
     2161 gccaagggct gatctggcca agggctgatc tggccaagaa gctgatctgg ccaagaaagc
     2221 gatctcgccg acgcgctacc gccccggcac gccgaggccc gtcgtggtcg tcggcggcgg
     2281 cgggcccggc ccgccacccc ccccgccctg gtccgccggc gcggtggtcg aagtagttgt
     2341 cgtcggcggg gctacggcga cgggtggggc cgccgggatc tcgtagggcc cgccgctgcc
     2401 ggtgccgccc gggtagaggc ggctgccggg gtcgatcccg gcccgggcca ccttgttgat
     2461 cgcatccacg atcttctgga tgggtggcgg ctggttgaag tcggtgctcg gcacgttggc
     2521 cagcgcctcg gacatgaagt tgtgccaggt ctgagcgggg atgccgccgc cgaagatcaa
     2581 ggggtaggtc tggccgaagg cgcggaggtt gcgcatgga
//
LOCUS       DASWGG010000162         4904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2735823, whole genome shotgun sequence.
ACCESSION   DASWGG010000162 DASWGG010000000
VERSION     DASWGG010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2735823"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VF954_02910"
     CDS             complement(<1..709)
                     /locus_tag="VF954_02910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9530060.1"
                     /translation="MRVALVCPYSLTLPGGVQGQVLGLARSLRALGHDTRVLAPSDGP
                     PPDPGVTSLGRSVPLSSNGSVAPIAPDFPCALRTIRTLRDEGFDVVHLHEPLVPGPTL
                     TTLMFSDAPLVGTFHRAGASSAYAFLAPVARSVANRLALRCAVSEHAGATAQAALGGA
                     YELLFNGIEIDRFAKATAWPTSGPTIFFVGRHEPRKGLATLLEAFARLPASTHLWVAG
                     DGPQTAELRRATAGEPRI"
     gene            complement(1046..1951)
                     /locus_tag="VF954_02915"
     CDS             complement(1046..1951)
                     /locus_tag="VF954_02915"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HEX9530061.1"
                     /translation="MPRLRDRAPYLAYRAAAAVGRGLPFPLARATADTASLLASRAMP
                     GRRAMVARHLRRVRPELEGLALERAVAESFASYGRYWLDSFRLPGTPADVIDARMSHV
                     GVDHLDDALALGKGVILAVPHLGGWDFGGAWLAAVGRPPTAVVEPVEPPELFAWFVGL
                     REALGMRIVPLGPRSATEVLRTLRAGGIVLLVSDRDLGGHGVEVELFGERTTLPGGPA
                     TLALRSGAPLMAAAVFLGRTGRHDGVILRPIDTSRGGGPLRDDVARVTQDLAHDLEKL
                     IRMAPEQWHLFQPNWPSDRAGPRGG"
     gene            complement(1973..2743)
                     /locus_tag="VF954_02920"
     CDS             complement(1973..2743)
                     /locus_tag="VF954_02920"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9530062.1"
                     /translation="MIDAHWRTTLERGTKPVGEGLRRAGVRADHLTALGLGMAGATCV
                     VIATGHLGWGLALLILSAVPDMLDGAVAKASGTSWARGSFFDSVADRLTDGLVLGGVA
                     WYLAGRRGGGRAAILPMAVMGASLLISYERAKAESLGYTARGGLMERAERVVALCAGL
                     AFSALLVPVLWLMLGLTAVTAVQRFVMVWRQAGAPPEPAPVVPRWRARADRSEPRWWT
                     RPERDPARETPTARWRARRQAAAASGSRQRVRFRSTRP"
     gene            2935..>4904
                     /gene="fusA"
                     /locus_tag="VF954_02925"
     CDS             2935..>4904
                     /gene="fusA"
                     /locus_tag="VF954_02925"
                     /inference="COORDINATES: protein motif:HMM:NF009891.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX9530063.1"
                     /translation="MKTFPPVRIRNVALVGHGGAGKTTLAEALLHCAGAISRLGRVEE
                     GTTTTDFDPEEVKRHISLSLSLAAFEYQGYKLNLLDTPGYADFVTDVDAALSVADMAV
                     FVVSAVEGVEVQTEAIWRMAAARGLPRMIFVNKLDRERASFDRTLDQLREVFGAGVAP
                     LELPVGEEGAFRGVADLLTDTAITYDTGQPVIGEIPDDMESREHQVHDNLVEGIVVAD
                     DDLMERYLEGDVPNPKELEDTLAHGVASASVFPVVCGSAAKGIAIDRLATFLCEIGTA
                     PTDRPPAAVRAGDTTSEVPCDAAGQPLGYVFKTIADPYVGKISMLKVLSGTIKPDTVL
                     TNPRTHADERLHALFTLRGKEQDTLGEAAAGDLLAVAKLSDTGTGDTLAPKGTPVVVA
                     PPPPPVPVLSVAIRPRSKGDDDKLMTALHRLQDEDPALQVRRDDETHQTLLSGMGETH
                     LAIVTERLQRKFGVDVETEEVRVAYRETITRAAEAEGRYKKQTGGHGQFGVANLRIEP
                     LERGAGFEFADAVVGGAIPRQFIPAVEKGVLETMELGGVFGYPVVDVKVTVHDGKYHS
                     VDSSEMSFRMAGSLGFKEAMEKAGPVMLEPVSLLEVTVPLAYQGDVMGDLNARRGRVQ
                     GTETGEAGEQIIIALVPTSEILRYAIDLRSLTG"
BASE COUNT          765 a         1821 c         1754 g          564 t
ORIGIN      
        1 cgatgcgggg ctcacccgcc gtcgcccgcc gcagctcggc cgtctgcggc ccgtcgccgg
       61 ccacccagag gtgggtcgag gccggcagcc gggcgaaggc ctccagcagg gtggccaacc
      121 ccttccgggg ctcgtgccgg cccacgaaga agatggtggg gccgctcgtc ggccaggcgg
      181 tggccttggc gaagcggtcg atctcgatgc cgttgaagag cagctcgtag gcgccaccga
      241 gggccgcctg cgccgtcgcc ccggcgtgct cggacaccgc gcaccgcagg gccaggcggt
      301 tggcgacgct gcgggccacc ggggccagga aggcgtaggc cgagctggct ccggcccggt
      361 ggaacgtgcc caccaagggg gcgtcgctga acatcagggt ggtcagcgtc ggcccgggca
      421 ccaggggctc gtggaggtgc acgacgtcga acccctcgtc gcgcagcgtg cggatggtgc
      481 gcagcgcgca cggaaagtcc ggcgcgatcg gggcgacgga cccgttggag gaaaggggca
      541 ccgacctgcc gagcgacgtc acgcccggat cgggcggtgg cccgtcggac ggagccagga
      601 cccgggtgtc gtggcccagg gctcgcaggg aacgggccaa gcccagcacc tggccctgga
      661 ccccgccggg cagggtgagg ctgtaggggc agaccagggc cacgcgcacc caaccacgtt
      721 agagcctggc caataccgcc gccgtaccgc cggcgcgacg tcccgtcagt ccgcctggga
      781 ccgcccgcca ctgggccaca gcctggcatc gccctggccg cggcccggct tcgggacacg
      841 aaaaccattc ttcaagcaat aaaggcgcta tagtgatata aaagcatgga agaatggttg
      901 agagggggcc ccgatggcgg ccagaggtgg ccaaagccgc cccggccggg cgccagccgc
      961 cgccgggatc cccccacatc ggtgcccggc ggtgcccggc ggtcgagcgc cgggtcgcgc
     1021 accgccaccc gcaggtccac ccggctcagc cgccgcgggg accagcgcgg tcgctgggcc
     1081 agttgggctg gaacaggtgc cactgctccg gcgccatgcg gatcagcttc tcgagatcgt
     1141 gggcgaggtc ctgggtcacc cgggccacgt cgtcgcgcaa cggtccaccg ccccgcgagg
     1201 tgtcgatggg ccggaggatg accccatcgt ggcggccggt ccgccccagg aacaccgcgg
     1261 cggccatgag cggcgccccg ctgcgcaggg cgagggtggc cggtccgccc ggcagcgtgg
     1321 tccgctcgcc gaagagctcg acctcgacgc catggccccc gaggtcccgg tccgacacca
     1381 gaaggacgat gccgccggcc cggagcgtgc ggagcacctc ggtggccgaa cggggcccga
     1441 gggggacgat cctcatgccc agcgcctctc gcagcccgac gaaccatgcg aacagctcgg
     1501 ggggctcgac gggctcgacc acggcggtgg gcgggcggcc cacggccgcc agccaggccc
     1561 ccccgaagtc ccagcccccc aggtggggca cggccaggat gactcccttg cccagggcca
     1621 gggcgtcgtc gaggtggtcg acgcccacgt gggacatgcg cgcgtcgatg acgtcggccg
     1681 gcgttccggg caggcggaag gagtccagcc agtaccggcc gtaggaggcg aagctctcgg
     1741 ccacggcccg ctccagggcc aggccttcca gctcgggccg gacgcggcgg aggtggcgcg
     1801 ccaccatggc gcgccgcccc ggcatcgccc gtgaggccag caacgacgct gtgtccgcgg
     1861 tggcccgggc gagggggaag ggcaggccgc gcccgaccgc ggccgcggcc cggtaggcga
     1921 ggtacggagc gcggtccctc aggcgcggca cagcccgttc cggcgtaggc ggttacggcc
     1981 gggtgctgcg gaaccgcacc cgctggcgcg acccgctggc ggcggccgcc tgccgccgcg
     2041 cccgccagcg cgccgtcggc gtctccctcg ccgggtcgcg ctcgggccgg gtccaccagc
     2101 ggggctccga ccgatcagcc cgggcccgcc agcggggcac cacgggtgcc ggctcgggcg
     2161 gcgccccggc ctggcgccac accatcacga accgctggac ggccgtgacg gcggtcagac
     2221 cgagcatcaa ccacagcacg gggaccagca gcgcgctgaa cgccagcccg gcgcacaggg
     2281 ccaccacccg ctcggcacgc tccatgagcc cgccccgggc ggtatagccg agggactcgg
     2341 ctttcgcccg ctcataggag atcaacaggg aggcgcccat gaccgccatg gggaggatgg
     2401 ccgcccggcc gccaccccgc cggccggcca ggtaccacgc caccccaccg agcaccagcc
     2461 cgtcggtcag gcggtcggcg acggagtcga agaacgaccc ccgcgcccac gacgtccccg
     2521 acgccttggc caccgcgccg tccagcatgt cgggcacggc cgagaggatc agcagggcga
     2581 gaccccaccc gagatgcccg gtcgcgatca cgacacaggt cgccccggcc atccccagcc
     2641 cgagggcggt caggtggtcg gcgcgcaccc cggcccggcg caggccctca ccgactggct
     2701 tggtccctcg ctcgagggtg gtgcgccagt gtgcatcgat catgaacggg gctccaagag
     2761 gtgctcgaca tccgccaggc taccacccgg gtgcaccctc accgcgaact ccagcaagca
     2821 cctcgcccga cggacggggc agaagcacgg cggcggcggg tccaataatc tggcctcccc
     2881 ggcggcctat cgccacccaa ccgagagcaa ccgaaccgag aacgaggtcc aagcgtgaag
     2941 acgttccctc cggtcaggat ccgcaacgtc gccctggtgg gccacggtgg agcgggcaag
     3001 acaaccctgg ccgaggccct cttgcactgc gccggcgcca tctcccgcct gggccgggtg
     3061 gaggaaggga ccaccacgac cgacttcgac cctgaggagg tcaagcgcca catctcgctg
     3121 tcgctctcgc tggccgcgtt cgagtaccag ggctacaagc tcaacctgct cgacaccccg
     3181 ggctacgccg acttcgtgac cgacgtggac gccgccctgt cggtggccga catggcggtc
     3241 ttcgtcgtca gcgcggtcga gggcgtggag gtgcagaccg aggccatctg gcgcatggcc
     3301 gcggcccgcg gcctgccccg catgatcttc gtcaacaagc tcgaccggga gcgggccagc
     3361 ttcgatcgca ccctggacca gctccgggag gtgttcggcg ccggcgtcgc ccccctcgag
     3421 ctgcccgtag gtgaggaggg cgcgttccgg ggcgtcgccg acctgctgac cgacaccgcc
     3481 atcacctacg acacgggcca gccggtgatc ggggagatcc ccgacgacat ggagtcccgg
     3541 gagcatcagg tccacgacaa cctggtcgag ggcatcgtag tggccgacga cgacctcatg
     3601 gagcgctacc tcgaaggcga cgtgccgaac cccaaggagc tggaggacac gctggcccac
     3661 ggcgtggcgt cggcgtcggt gttcccggtc gtctgcggat cggcggcgaa ggggatcgcc
     3721 atcgaccggc tggccacctt cctctgcgag atcggcaccg cccccacgga ccgcccaccc
     3781 gccgccgtgc gggccggcga cacgacctcc gaggtgccgt gcgacgcggc gggccagcca
     3841 ctggggtacg tgttcaagac catcgccgac ccctacgtgg gcaagatctc catgctcaag
     3901 gtgctgtcgg gcaccatcaa gcccgacacc gtgctgacca atccgcggac acacgccgac
     3961 gagcgcctgc acgcgctctt caccctcagg ggcaaggagc aggacacgct cggcgaggcc
     4021 gcggccgggg acctcctggc cgtggccaag ctatccgaca ccggcaccgg cgacaccctc
     4081 gcccccaagg gcacgcccgt ggtcgtggcg cctccgccgc cgcccgtgcc cgtgctgagc
     4141 gtcgccatcc ggccccggtc caagggcgac gacgacaagc tgatgaccgc cctgcaccgc
     4201 ctccaggacg aggatcccgc cctccaggtc cgccgcgacg acgagaccca ccagacgctg
     4261 ctgtccggca tgggcgagac ccacctggcc atcgtgaccg agcgcctcca gcgcaagttc
     4321 ggcgtcgacg tcgagaccga ggaggtccgg gtcgcctacc gcgagacgat caccagggcc
     4381 gccgaggccg agggtcggta caagaagcag accggcgggc acggccagtt cggggtggcc
     4441 aacctgcgga tcgagcccct ggagcggggc gccggcttcg agttcgccga cgcggtggtc
     4501 ggtggcgcca tcccccgaca gttcatcccg gccgtggaga agggcgtgct cgagaccatg
     4561 gagctgggcg gggtcttcgg ctaccccgtg gtggacgtca aggtcaccgt gcacgacggc
     4621 aagtaccaca gcgtcgactc ctccgagatg agcttccgca tggccggctc cctcgggttc
     4681 aaggaggcga tggagaaggc gggccccgtc atgctcgagc cggtgtcgct gctcgaggtc
     4741 acggtcccgc tcgcctatca gggcgacgtg atgggcgacc tcaacgcccg ccggggaagg
     4801 gtccagggca cggagaccgg ggaagccgga gagcagatca tcatcgccct ggtgcccacg
     4861 tccgagatcc tgcgctacgc catcgacctg cgctcgctca ccgg
//
LOCUS       DASWGG010000163         7619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1447472, whole genome shotgun sequence.
ACCESSION   DASWGG010000163 DASWGG010000000
VERSION     DASWGG010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447472"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VF954_02930"
     CDS             <1..254
                     /locus_tag="VF954_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530064.1"
                     /translation="EAWSSSLLEAPVVLGVDRADQDATVVRVAAKLAVGQSDAFARAL
                     RSRVVARLAHEGLTWAPAAASPASPASPASPASPASPGG"
     gene            416..874
                     /locus_tag="VF954_02935"
     CDS             416..874
                     /locus_tag="VF954_02935"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9530065.1"
                     /translation="MKPGDRAPDFELPDQAGQPVRLQEELAKGPVVLFFYPKAMTSGC
                     TAESCHFRDLAAEFADVGARRLGISADAVDRQAAFAEKNSLDYPLLSDTDRRAARLYG
                     VKRPGPLWNRRTTFVIAQDGVVLDVIASETDMEVHADRALATLRAHPTGD"
     gene            complement(918..1607)
                     /locus_tag="VF954_02940"
     CDS             complement(918..1607)
                     /locus_tag="VF954_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530066.1"
                     /translation="MGLAQVADDLWVDEVVVVAARGPDPLTLLAAAAQVTTTVGLVAG
                     LDASVAVPDATAAAAATALTLGVGRVALLIEGCAGARDRAEALVDACRAASPARLPIY
                     VAGPPLPEVGQLAATVADGWQFPPGLPLDQVGLAATAVGERRLGTGRPRALEVRTVLT
                     PTGTDGADGADAMVWDLGQVAAHFAAGAAVVVADLDDAVARGVDPRVAIVEVAQAFAD
                     RPARPARPARP"
     gene            1813..2625
                     /locus_tag="VF954_02945"
     CDS             1813..2625
                     /locus_tag="VF954_02945"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX9530067.1"
                     /translation="MASLDGFTIGITADRRWKEQADLFTRRGAAVLHGPTLATRFLSD
                     DPDLRAATHALVERPPDLLIATTGVGIRAWLEAAQTWGIADELLAALGAARTVARGPK
                     AANALAQAGLPVHMRAKSERMEEVAELLRSEPAASGRVALQRYGEDSLDAQAVLDARG
                     ADVVALSVYQWQLPADTGPARGLVQAACSGEVDVVTFTSAPAVVNLLGIAAEIGARRE
                     LLGAFAGGRVTAGCVGPVCAAAARDAGIADPIGPAVGRLGLLVRMVEDRLTA"
     gene            3167..4486
                     /locus_tag="VF954_02950"
     CDS             3167..4486
                     /locus_tag="VF954_02950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9530068.1"
                     /translation="MQPGLATVADVVAPVIRAFIGADPPFAFRFWDGSEVGPGTAAAT
                     GAATVVVHGPEAIRRILFAPNELGLGRAYVAGELDIEGDVYAALDLRAYVAARDQRAR
                     VALDVRGWGEVVRAARRLGILGLPLRPPPEEARLHGRRHSKERDAAAVAHHYDAGNDF
                     YRLVLGPTLTYSCAYFASEDQPLDDAQEHKHDLVCRKLGLRPGMRLLDVGCGWGTMVL
                     HAATHHDVHAVGVTLSRQQADLAAARVADAGLTGRVQIRYQDYRDIADGPYDAISSIG
                     MFEHVGAARLSEYFQQLFTLLRPHGRLLNHAISRPPGDAHPDRHSFIERYVFPDGELQ
                     EVGALVSGMQANGFEVRDVQSLREHYALTLRHWVANLDADWEKAQQFAGPARARVWRL
                     YMAASALNFEAGRANLHQVLGVKVDDAGGSGVPLTRHELMQGAVSVR"
     gene            complement(4503..5975)
                     /locus_tag="VF954_02955"
     CDS             complement(4503..5975)
                     /locus_tag="VF954_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9530069.1"
                     /translation="MTYTDAQFLALFEPRGVVVAGASTHPGKFGFVTLHNLLRHDYPG
                     RVFATSLSGDEVLGIPTLTSLDQLPGGEADLVFVCTPVAANPDLLRACAARGVRAAFV
                     TTAGYGEAGEEGRAAERELVALAAELGLLLVGPNGQGVVSTPASLCAQIVAPYPPAGR
                     IGVVSQSGNFVSSFMNYAIQSGIGISRAVSAGNAAATGVTDFLEFYASDPATAVSLSY
                     VEGMTDARAFFERVSPLAARQPLVLLKGGTTAGGQRAAASHTGSLATDDRIFDGACRQ
                     AGIVRAATVEEAFEAAATFASQPLPRGPRTVVLTTAGGWGVVTADAINRTSLVLQPLP
                     DDLRAALDHLLPPRWSRNNPVDLAGGETRDTIPEVLGLIAGHPEVDAVIYLGLGIQSN
                     QARMMRTGRFYPGHGLERIVAYHERQDARFAEAAARISDVTGKPVLTATELAVASPDN
                     PGPATVRDTGRLCYASANRAVIALDHLWRHARWRQARGLA"
     gene            complement(5972..6616)
                     /locus_tag="VF954_02960"
     CDS             complement(5972..6616)
                     /locus_tag="VF954_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX9530070.1"
                     /translation="MTTLSEAQSKRMLAAYGVPVAREETASTAAAAGEAADRIGYPVV
                     VKLCGASIAHKTERGLVRVGLADRPSVEAAAVAMLGMAQPGDGEVEILVAEMVGGSRE
                     LIAGLVRDPQFGPCVMLGVGGILAEAVADVAFRLVPLSGVDAHDLVDELGTRRLFGEF
                     RGEPAVDRDRLAAVVAGLSTLAGEHPEVVSVDINPLIVAGGVPVAVDALVELRP"
     gene            complement(6695..>7619)
                     /locus_tag="VF954_02965"
     CDS             complement(6695..>7619)
                     /locus_tag="VF954_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530071.1"
                     /translation="ISRHGYRFVASDGGVFDEGDAAFFGSTGAIKLNKPVVGQAATST
                     GLGYWNIAADGGMFSFGDAGFFNSLPGLHVTPNKPVVGLLATPFDDGYAAIGSDGGLF
                     AFGAFPFLGSTGGIKLNAPVVGGAINVANPGYYEVASDGGIFAFQAPFLGSMGGQHLN
                     KPIVGMATTPDGGGYWLVASDGGIFSFGDAQFFGSTGGLTLNKPIVGMGASPSGFGYW
                     LVGSDGGIFSFGDAKYQGSTGNITLTQPVVGISTMPATATSSAIIANIPGSPPPFAST
                     TPAFTFADDGGFFPYHCSIHDNMFGVIQVST"
BASE COUNT         1070 a         2842 c         2705 g         1002 t
ORIGIN      
        1 acgaggcctg gtcctcctcg ctgctcgagg cgcctgtggt cctcggcgtg gaccgcgccg
       61 accaggacgc gaccgtcgta cgggtggcgg ccaagctcgc ggtcggccag tcggacgcct
      121 tcgcccgggc gcttcgctcc cgcgtcgtgg cccgattggc gcacgagggg ctcacctggg
      181 caccggcggc cgcctcaccc gcctcgcccg cctcgcccgc ctcacccgcc tcacccgcct
      241 cacccggcgg ttgaccggcc cctgcaatgg gttaaactca ttcttcaagc agaaaaacca
      301 ctgggacggc attaaagcat ggaagaatgg ttggaccgac cctgttccgc ctgcgcccgc
      361 ctggcccatg atcccagtgg tccccatggc aaagatgggc attaggctga ccgccgtgaa
      421 gccgggtgac cgtgcccccg atttcgagct ccccgaccag gccggccagc ccgtccgcct
      481 ccaggaggag ctggccaaag ggccggtcgt gctgttcttc taccccaagg cgatgacctc
      541 cggctgcacc gcggagagct gccatttccg cgacctcgcc gccgagttcg ccgacgtcgg
      601 ggcccggcgg ctggggatca gcgccgacgc cgtagaccgc caggccgcgt tcgccgagaa
      661 gaacagcctg gactacccgc tgctgtccga taccgaccgg cgggcggcgc gcctctacgg
      721 ggtgaagcgg cccggtcccc tttggaaccg gcgcaccacc ttcgtgatcg cccaggacgg
      781 cgtggtgctc gacgtgatcg ccagcgagac cgacatggag gtccacgccg accgggccct
      841 cgctacgctc cgggcccatc cgaccggcga ctagggcgac gagagagcac accgaccggt
      901 gggcgcccgg cggggcgcta cgggcgggcc gggcgggccg ggcgggccgg gcggtcggcg
      961 aacgcctgcg ccacctcgac gatcgccacg cggggatcca cgccccgggc cactgcgtcg
     1021 tcgagatcgg ccaccactac cgccgcaccc gcggcgaagt gggcggcgac ctggccaagg
     1081 tcccagacca tggcgtcggc gccgtcggcg ccgtcggtac cggtcggcgt gagcaccgtg
     1141 cgcacctcca gggcacgggg acgccccgtg cccaagcggc gctcgccgac ggccgtcgcc
     1201 gccagcccga cctggtcgag gggcaggccg ggcgggaact gccagccgtc ggcgaccgtg
     1261 gcggccagct gcccgacctc gggcaggggc gggccggcga catagatggg cagacgagcc
     1321 ggcgacgcag cccggcacgc gtcgaccaga gcctcggccc ggtcccgcgc ccccgcgcac
     1381 ccctcgatga gcaacgccac ccggcccacg ccgagcgtga gagcggtggc cgcggcggcg
     1441 gcggtcgcgt ccgggacggc cacggacgca tccagtcccg caaccaaccc aacggtggtg
     1501 gtgacctggg cggccgcggc cagcagcgtc agcgggtcgg ggccccgggc ggccaccacg
     1561 acgacctcgt cgacccagag gtcgtcggcg acctgggcca ggcccaccag gctacgaacc
     1621 tcggccgcag ccagagtacg actcggcagc atgacggcga ggcggggcag ccggcgggcg
     1681 atgccacgac cgtacagcca ccgcgaccgt gcagcccccg cgaccgtgca gcccccgcga
     1741 ccgtgcagtc cccgcgaccg acacggcccc gtacgtacgg cgcagcccat gtcggccggt
     1801 actgtgatcg gcgtggcctc actcgacggg ttcaccattg ggatcacggc cgaccgcagg
     1861 tggaaggagc aggccgacct gttcacccgc cggggcgcgg ccgtcctcca cgggcccacg
     1921 ctcgccaccc ggttcctctc cgacgacccc gacctccggg ccgcgaccca tgccctggtc
     1981 gagcggccac cggacctgct gatcgccact acgggtgtcg gcatcagggc ctggctcgag
     2041 gccgcccaga cgtgggggat cgcagatgag ctcctcgctg ccctgggagc ggcgcgcacc
     2101 gtggcgaggg gaccaaaggc ggccaacgcc ctggcccagg ccggcctccc cgtccacatg
     2161 cgggccaagt ccgagcgcat ggaggaggtg gccgagctac tcaggtcgga gccggcggcc
     2221 agcgggcgcg tcgccctgca gcgttacggg gaggacagcc tggacgcgca ggcggtgctc
     2281 gacgcccggg gcgcggacgt cgtcgccctg tccgtgtacc agtggcagct gccggccgac
     2341 accggaccgg cccgcgggct ggtgcaggcc gcctgttcgg gtgaggtcga cgtggtgacc
     2401 ttcacgagcg cgcccgcggt cgtcaacctc ctcggcatcg ccgcagagat cggcgcgcgc
     2461 cgcgagctgc tcggcgcgtt cgcgggcggg cgggtcacgg ccggctgcgt cggtccggtg
     2521 tgcgcggcgg cggcccgcga tgccggcatc gccgatccga tcggacccgc cgtgggccgc
     2581 ctcggcctgc tcgtgcggat ggtcgaggac cgcctgacgg cctgaccgcc tgaccgcctg
     2641 acggcctggc cgcccgacgg cctgcgcgcc cagggtcggg ggcgagcccc cggacgcgac
     2701 acgaccccga gtagccgctc ggcgaacctc acgcacttct cggggtcgta cctcagcttg
     2761 gtctccctcc gggcgctcgc cgggtggcgc cgcttccggc cgacccaccg cccagaagga
     2821 cggttggtgc tttcggatcc cttacctgac cggccgttcc acacccggct cgttgttcgg
     2881 agtcaccttg cggcagcccc tcccggcgat cccgttggtt ggcgcttcgc tgtccggctg
     2941 agagcactgt gctcccggcg tcttccggcg tcaagggccc cgcgggaaat cccgagcttg
     3001 tccccagaac ttcttgcccg tccccaggat gtccaccgac cggcgcctcc ttgtcccccg
     3061 gatgtccccc gcccgatctt caacgatcgc ttcgacaatc gcatggccct gcggccgctc
     3121 ccggctcata atctcatcca cgcggaacca acgaggaggt cagtgcatgc agcccggtct
     3181 cgccactgtg gccgacgtcg tcgctccggt catccgggcc ttcatcgggg ccgatccccc
     3241 gttcgcgttc cggttctggg acgggagcga ggtgggcccg ggcaccgcgg ccgctacggg
     3301 tgccgcgacg gtggtcgtgc acggccccga ggccatccgg cgcatcctct tcgccccgaa
     3361 cgagctgggc ctgggccggg cctacgtggc cggcgagctc gacatcgagg gcgacgtgta
     3421 cgccgcgctc gacctgcggg cgtacgtcgc ggcccgcgac cagcgggccc gggtcgccct
     3481 cgacgtccgg gggtggggcg aggtcgtccg ggcggcgcgg cgcctcggca tcctcggcct
     3541 acccctccga ccgccgcccg aagaggcccg cctccacggc cggcgccatt ccaaggagcg
     3601 cgacgctgcg gcggtcgccc atcactacga cgcgggcaac gacttctacc gcctggtcct
     3661 cggcccgacg ctgacctact cgtgcgccta cttcgcctcc gaagaccagc ccctcgacga
     3721 cgcccaggag cacaagcacg acctggtctg ccgcaagctc ggcctccgcc ccggcatgcg
     3781 gctgctcgac gtggggtgcg gctgggggac catggtactc catgcggcca cccaccacga
     3841 cgtgcacgcc gtgggcgtga ccctgtcccg ccagcaggcc gacctggccg cggctcgggt
     3901 cgccgacgcc ggcctgaccg gccgggtcca gatccggtac caggactacc gcgacatcgc
     3961 cgacgggccc tacgacgcca tctccagcat cgggatgttc gagcatgtgg gcgcggcccg
     4021 cctgagcgag tacttccagc agctgttcac gctgctgcgc ccccacggcc ggctgctcaa
     4081 ccacgccatc tcccggccgc cgggcgacgc tcacccggac cgccattcgt tcatcgagcg
     4141 ctacgtcttc cccgatggcg agctacagga ggtcggggcc ttggtctccg gcatgcaggc
     4201 caacggcttc gaggtgcgcg acgtccaatc cctgcgggag cattacgccc tgacgctgcg
     4261 ccactgggtc gccaacctcg acgccgactg ggagaaagcc cagcagtttg ccggaccagc
     4321 ccgcgcccgg gtctggcgtc tctacatggc cgcgtccgcc ctcaacttcg aggccggccg
     4381 ggccaacctg caccaggtcc tcggggtcaa ggtcgatgat gccggcggct ccggcgtgcc
     4441 gctcacgcgc cacgagctga tgcaaggcgc cgtctccgtg aggtagcccc gggttcccgg
     4501 cgtcaggcca ggccacgagc ctggcgccac cgggcgtggc gccacaggtg gtcgagggcg
     4561 atgaccgccc ggttggccga cgcgtagcac agccgcccgg tgtccctgac cgtcgccggc
     4621 ccagggttgt ccggcgaggc gacagccagc tcggtggccg tgaggacggg cttgccggtc
     4681 acgtcggaga tccgggccgc ggcctcggcg aagcgggcgt cctggcgctc gtggtaggcc
     4741 acgatgcgct cgaggccgtg gcccgggtag aagcgcccgg tcctcatcat ccgggcctgg
     4801 ttcgactgga tgccgaggcc taggtagatg acggcgtcca cctccgggtg gccggcgatc
     4861 agccccagga cctccgggat ggtgtcgcgc gtctcgcccc ccgccaggtc cacggggttg
     4921 ttccggctcc accggggcgg gagcagatgg tcgagggccg ccctgaggtc gtccgggagc
     4981 ggttggagca cgaggctggt gcggttgatg gcgtctgcgg tgaccacacc ccaccccccc
     5041 gcggtcgtca ggaccaccgt ccgggggccg cgcggcagcg gctgcgaggc gaacgtggcc
     5101 gcggcctcga aggcctcctc cacggtggcg gcccgcacga tcccggcctg ccggcacgct
     5161 ccatcgaaga tccggtcgtc ggtcgcgagc gagccggtgt gggaggccgc ggcccgctgc
     5221 ccgccggcgg tcgtcccgcc cttgagcagc acgagcggct ggcgggcggc cagggggctc
     5281 acccgctcga agaaggcccg ggcgtcggtc atgccctcca cgtaggacag cgagacggcg
     5341 gtggccggat cggacgcgta gaactcgagg aagtcggtca cgcccgtggc cgcggcgttg
     5401 cccgccgaga ccgcccggct gatcccgatg ccgctctgga tggcgtagtt catgaacgac
     5461 gacacgaagt tgccggactg gctcaccacc ccgatccgcc ccgccggcgg gtagggcgcc
     5521 acgatctggg cgcagagcga tgcgggcgtg gagaccacgc cctggccgtt gggcccgacg
     5581 agcaggagcc cgagctcggc ggccagggcc accagctccc gctcggccgc cctgccctcc
     5641 tcgcctgcct ccccgtagcc ggctgtcgtc acgaaggcgg cccggacgcc cctggccgcg
     5701 cacgcccgca acaggtcggg gttggccgcc acgggggtgc acacgaagac caggtcggcc
     5761 tcgcccccgg ggagctggtc gagcgaggtc agcgtaggga tgccgaggac ctcgtcgccc
     5821 gacagcgacg tggcgaagac ccgccccggg tagtcgtggc gcagcaggtt gtgcagggtc
     5881 acgaagccga acttgcccgg gtgcgtgctc gccccggcca ccaccacgcc ccggggctcg
     5941 aagagggcga ggaactgcgc atcggtgtac gtcacgggcg aagctcgacc agggcgtcga
     6001 cggccaccgg cacccccccc gccacgatca gcgggttgat gtcgaccgag accacctcgg
     6061 gatgctcgcc cgccagcgtt gacaaccccg ctaccacggc cgccagccgg tcccggtcca
     6121 cggccggctc gccccggaac tcgccaaaga ggcgtcgggt gcccagctcg tcgaccaggt
     6181 cgtgggcgtc gacgcccgag agcggcacca gcctgaaggc cacgtcggcc accgcctcgg
     6241 ccaggatgcc tccgacgccg agcatgacgc acggcccgaa ctgcggatcg cgcaccagcc
     6301 cggcgatcag ctcgcggctg ccgccgacca tctcggccac caggatctcg acctccccgt
     6361 cacccggctg cgccatcccc agcatcgcga cggcggcggc ctcgacggag ggccgatcgg
     6421 ccagcccgac tcgcaccagt ccccgctcgg tcttgtgggc gatggaggcg ccgcacagct
     6481 tcaccacgac cgggtagccg atgcggtcag ccgcctcgcc cgcggccgcg gccgtcgagg
     6541 cggtctcctc ccgggcgacg gggacgccgt aggcggcgag catgcgcttg gactgggcct
     6601 cggagagggt ggtcacgggc cgggatcata gggtgggcgc gccgaggccc gggccggcgg
     6661 cccgggcctc atgcgtgcgt cctggccggg cgggtcaggt cgagacctgg atcaccccga
     6721 acatgttgtc gtggatcgag cagtgatagg ggaagaaccc gccgtcgtcg gcaaaggtga
     6781 aggccggcgt cgtgctcgcg aacgggggcg gggagcccgg gatgttggcg atgatggccg
     6841 acgaggtggc cgtggccggc atggtcgaga tgcccaccac cggctgggtc agcgtgatgt
     6901 tcccggtcga gccctggtac ttggcatcac cgaaggagaa gatgccaccg tcggagccga
     6961 ccagccagta gccgaacccg ctcggggagg cgcccatgcc gacgataggc ttgttgaggg
     7021 tgaggccccc ggtcgagccg aagaattggg cgtcaccgaa ggagaagatc cctccgtcgg
     7081 atgccaccag ccagtagccc ccgccatcgg gcgtggtggc catcccgacg atcggcttgt
     7141 tgaggtgctg gccgcccatc gatcccagga acggcgcctg gaaggcgaag atgccgccgt
     7201 ctgaggccac ctcgtagtag cccggattgg cgacgttgat ggcccccccc acgaccggcg
     7261 cgttgagctt gatgccgccc gtgcttccta ggaagggaaa ggccccgaaa gcgaacaggc
     7321 cgccgtctga gccgatggcg gcgtagccgt cgtcgaatgg cgtggccagc aggccgacca
     7381 ccggcttgtt gggggtgacg tgcagcccgg ggagcgagtt gaagaacccg gcgtcgccga
     7441 aggagaacat ccctccgtcg gcggcgatgt tccagtagcc cagtccggtc gaggtggcgg
     7501 cctggccgac gacaggcttg ttcaacttga tggcgccggt gctgccgaag aaggcggcgt
     7561 ccccttcgtc gaacaccccg ccgtcagacg ccacgaaccg gtagccgtgg cgggagatg
//
LOCUS       DASWGG010000164         4205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1448559, whole genome shotgun sequence.
ACCESSION   DASWGG010000164 DASWGG010000000
VERSION     DASWGG010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448559"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..956)
                     /locus_tag="VF954_02970"
     CDS             complement(21..956)
                     /locus_tag="VF954_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9530072.1"
                     /translation="MVTTPDSPDTLRERSLREEALVSAHLHLVRVLVAEMANRVPRHV
                     FRDDLTSAAMSALAVAARHFDPARGVAFDRYAVRRMRGALLDELRAHDWASRSVRTRA
                     RQVSAATESLTAELGRRPSREEIADRCGLDREALASVNQDLYRASIFNYDAMVHDGTG
                     DELLPRDERSPDVVVLDNERKAYLIDAVAALPERLRQVVVGCFFEERSMQQLADSLGV
                     TQSRVCQIRAQALSMIREALESQLDAGPARQPDRGGPRVAKRRAAYLAAVEAGSDFRT
                     RVSYRDTVAGATSRLTRLTSGPPLEAAGAAPTAAA"
     gene            1218..1862
                     /locus_tag="VF954_02975"
     CDS             1218..1862
                     /locus_tag="VF954_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9530073.1"
                     /translation="MAPGLTAVVVDHWPLVRLGMARVIERVGLRVVAECDSGQEAVSH
                     ARRAGVALIALGSDLNDDPVATTTALVGLRDRPRVVVLVDHGDRERLAKLLEAGADGV
                     LVRTVGPDELGDAVQRVLAGERVLGTPLLPALVGMTTDALGAAGAGPEPTLTARERAV
                     LRALAGGRTNRDIAAELYMSEATVKTHLSHIYSKLGVTDRQQAVARALGLRLLA"
     gene            complement(1898..2995)
                     /locus_tag="VF954_02980"
     CDS             complement(1898..2995)
                     /locus_tag="VF954_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEX9530074.1"
                     /translation="MRAAIMEAPSQDLTIASDVDVAPPQAGEVLVRMVACGVCHSDLH
                     FLDASLPAVPPAILGHEAAGVVEEVGPGVSHLQPGDKVILTLRPACGHCYWCIRGEGQ
                     LCPEATAANTATTADGSPRITSGGRAVSQPLGIAAFAERVVTQASGAVKVPEDTPLDI
                     ACVLGCAVQTGVGAALNTARIEEGATVMIVGLGGIGISIAQGARLAGASRVIGVDPLE
                     SRREQSLGFGVTDVVDPAAGDVAAAAMGLTGVGVDYAFDAVGRAALVESCIGATRPGG
                     TAVIVGVPRMDERVSVPALFFALGEKKLKGCFLGSSNFAREVPRLLSLWRAGRLDLEG
                     MITARRPLDDINLAFADMKAGTGLRTVIEMS"
     gene            3085..3426
                     /locus_tag="VF954_02985"
     CDS             3085..3426
                     /locus_tag="VF954_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9530075.1"
                     /translation="MKDGNRAPEVSVDALAVALEGGAPLVDVREVEEYLAGRVPGARL
                     VPLSELEARAREIPEDTRVYLICRTGARSLAAATALNRAGWDTVSVAGGTMAWVESGR
                     PIESAGQGPPT"
     gene            complement(3443..3841)
                     /locus_tag="VF954_02990"
     CDS             complement(3443..3841)
                     /locus_tag="VF954_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9530076.1"
                     /translation="MTRARLAWEGLVVSAVSAALAGPLLGACSHPASPFHPLEAGNPR
                     LGEAAIVHYGCGSCHTIPGVRGADSLMSPPLAHFGKRAFIAGRIGNTEPNLIRWIEDP
                     QAVDPGNVMPNLGVTDTDAANIAAYLERLE"
     gene            complement(3838..4026)
                     /locus_tag="VF954_02995"
     CDS             complement(3838..4026)
                     /locus_tag="VF954_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530077.1"
                     /translation="MTPEGTPEGRPAHGREAETEAEIERQAAREPDDPTTGREELELE
                     LLEEHESEQGGEIGEHLG"
BASE COUNT          561 a         1519 c         1565 g          560 t
ORIGIN      
        1 ggaacgccag cccgggtggc tcaggcggcc gcggtcggag ctgcgccggc ggcctccagc
       61 ggggggcccg acgtcaaccg ggtcaggcgg gaggtcgctc cggccaccgt gtcgcggtag
      121 ctgacgcggg tgcggaagtc cgagcccgcc tccacggccg ccaggtacgc agcccggcgc
      181 ttggccaccc gggggccgcc gcggtcgggc tgccgggccg ggccggcgtc gagctggctc
      241 tccagggcct cccggatcat ggagagcgcc tgggcccgga tctggcagac gcgcgactgg
      301 gtcacgccca gcgagtcggc caactgctgc atggaccgct cctcgaagaa gcagccgacc
      361 accacctggc gaagccgctc cggcagggcg gccacggcgt cgatcaggta cgccttgcgc
      421 tcgttgtcca gcacgacgac gtcgggcgag cgctcgtccc tcggaagcag ctcgtcgccg
      481 gtcccgtcgt gcaccatggc gtcgtagttg aagatcgagg cccggtacag gtcctggttg
      541 acggatgcca aggcctcgcg gtcgaggccg cagcgatcgg cgatctcttc tctggagggg
      601 cggcggccca gctccgcggt gagcgactcg gtagcggccg acacctggcg ggcccgggtc
      661 cgcaccgagc ggctggccca gtcgtgggct cgcagctcgt ccagcagggc gccgcgcatc
      721 cggcggacgg cgtagcggtc gaaggccacg ccgcgggccg ggtcgaaatg ccgagcggcc
      781 acggccagcg cggacatcgc cgccgacgtc aggtcgtccc ggaacacatg gcggggcaca
      841 cggttcgcca tctcggccac gagcacccgc accaggtgca ggtgcgccga cacgagcgcc
      901 tcttcccgca gggaccgctc cctgagggtg tctgggctgt ccggtgtcgt gaccattgcc
      961 ccaattgtcg ggccacgccc atgtcgggcg aatcggccgc acgggtgaaa gccgggccgg
     1021 cgcatggcca ggtcgggcgg tgacaccttc gccgcgcggg ccccccgagc cgatcctgcc
     1081 tctcgttcca tcgacggtga gagcaagaac ctgaggaaac ctccccaact ttgatgagat
     1141 cccttcatct ccgccctcgg cgcgctccgg cgcatcggct gcggggtgcc gagcccgcgt
     1201 ggctacgatc gctggccatg gcccctggcc tgactgcggt ggtggtggac cattggccgc
     1261 tcgtgcgcct cggcatggcc cgcgtcatcg agcgtgtcgg ccttcgggtc gtcgcggagt
     1321 gcgacagcgg ccaggaggcg gtgagccatg cccggagggc gggcgtcgct ctgatcgctc
     1381 taggaagcga cctgaacgac gatcccgtcg cgaccacgac cgccctcgtc ggcctgcgcg
     1441 accggccgcg cgtcgtcgtg ctggtggacc acggcgatcg cgagcggctc gccaagctgc
     1501 tcgaggccgg ggcggacggg gtcctcgtgc ggacggtcgg cccggacgag ctgggagatg
     1561 cagtgcagcg cgtgctcgcc ggcgagcggg tgctcgggac gcccctgttg ccagccctgg
     1621 tcgggatgac cacggacgcg ctcggggccg cgggcgccgg gccggagccg acgctcacgg
     1681 cccgggagcg cgccgtgctg cgcgccctgg cgggcgggcg caccaaccgg gacatcgcgg
     1741 ccgagctcta catgtccgaa gcgaccgtca agacccacct gagccacatc tactccaagc
     1801 tcggcgtcac cgaccgccag caggcggtgg cccgcgccct gggcctgcgc ctgctggcct
     1861 gacggcgcgg cggcgaacgg gcggcccggc cagcggctca ggacatctcg atgacggtgc
     1921 gcaggcccgt gcccgccttc atgtcggcga aggcgaggtt gatgtcgtcg aggggtcgcc
     1981 gggccgtgat catcccctcc aggtcgagcc gcccggcccg ccacagcgag agcagccggg
     2041 gcacctcccg ggcgaagttg gaagagccca ggaagcagcc cttcagcttc ttctccccga
     2101 gggcgaagaa cagcgcgggc accgagaccc gctcgtccat ccgcgggacg cccacgatga
     2161 ccgcggtgcc gcctggccgg gtcgccccga tgcacgactc gaccagcgcg gcgcggccga
     2221 ccgcgtcgaa ggcgtagtcg acgcccaccc ccgtcagccc catcgctgcc gcggccacgt
     2281 cgcccgccgc cgggtccacc acgtcggtga ccccgaagcc gagcgactgc tcgcggcgcg
     2341 actccagggg gtcgacaccg atcacccggc tggcgccggc gagccgcgca ccctgggcga
     2401 tggagatgcc gatgccgccg agcccgacga tcatcaccgt cgcaccctct tcgatccggg
     2461 cggtgttgag cgcggccccg accccggtct ggacggcgca tccgagcacg caggcgatgt
     2521 cgaggggcgt gtcctcgggg accttcaccg cacccgacgc ctgggtcacg acgcgctccg
     2581 cgaaggcggc gatccccagg ggctggctga cggcccggcc ccccgaggtg atccggggcg
     2641 acccgtccgc ggtcgtagcc gtgttggcgg ccgtggcctc cgggcacagc tggccctcgc
     2701 cacgtatgca ccagtagcag tgaccgcagg ccgggcgcag cgtgaggatg accttgtcgc
     2761 cgggttggag gtgcgatacg cccgggccga cctcctcgac gacgcccgcc gcttcgtggc
     2821 cgaggatggc ggggggcacg gccggcaggc tggcatcgag gaaatggagg tcggagtggc
     2881 acaccccgca cgccaccatc cggaccagga cctcgcccgc ctgcggggga gcgacgtcga
     2941 cgtcggacgc gatggtcagg tcctggctcg gggcttccat gatggcggca cgcatgaccg
     3001 gtgttctagc gcaccccggc ggggcccgta ccggcggggc tgcgctacct tcggcggcgc
     3061 ggctgcggac cgaggaggag ccacgtgaaa gacgggaatc gggccccgga ggtgagcgtc
     3121 gacgcgctgg ccgtggcgtt ggagggcggc gcgcccctcg tcgatgtccg cgaggtcgag
     3181 gagtacctgg cggggcgcgt ccccggcgct cggctcgtcc cgctctcaga gctcgaggcg
     3241 cgagcccggg agatccccga ggacacgcgg gtgtacctga tctgccgcac gggcgcgcga
     3301 agcctggcgg cagccaccgc cctcaaccgg gccgggtggg acaccgtctc ggtggccgga
     3361 gggacgatgg cctgggtcga gtcgggccgg cccatcgagt cagccgggca ggggccgcca
     3421 acctgatgcc cgggccgacg ggctattcga ggcgttcgag gtaggcggcg atgttggccg
     3481 cgtcggtgtc ggtcaccccc aggttcggca tgacgttgcc ggggtccacg gcctgggggt
     3541 cctcgatcca ccggatcagg ttgggctccg tgttgccgat gcgaccggcg atgaaagctc
     3601 gctttccgaa gtgggccagc ggcgggctca tcagcgagtc cgcgcccctc acaccgggga
     3661 tggtgtggca gctcccgcat ccgtaatgga cgatggccgc ctcccccagt cggggattgc
     3721 cggcctccag tgggtggaac ggggaggccg ggtggctgca ggcccccagc agcggcccgg
     3781 cgagcgccgc gctcacggcg ctcacgacca gcccctccca cgccaggcgc gcccgggtca
     3841 cccgaggtgc tcgccgatct ccccgccctg ctcggactcg tgctcctcca acagctcgag
     3901 ctcgagctcc tcgcgcccgg tggtcggatc gtcgggctca cgggccgcct gccgctcgat
     3961 ctcggcctcc gtctcggctt cccgaccgtg cgcgggtctc ccttctggtg tcccttctgg
     4021 tgtcatgggg caacgttgta gccgcgttgc ggcccgcatg ggccagcggg tgcagaccag
     4081 cctcctatgg gccgagctcg gccaggaggg caggcagccg agagaagaag accgacctgg
     4141 cgagcaccgg gtcgcagccg gcggcgcggg cggccgcggt tccctccgaa tcgacgtggg
     4201 agatg
//
LOCUS       DASWGG010000165         6565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3064955, whole genome shotgun sequence.
ACCESSION   DASWGG010000165 DASWGG010000000
VERSION     DASWGG010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3064955"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..781)
                     /gene="fabG"
                     /locus_tag="VF954_03000"
     CDS             complement(35..781)
                     /gene="fabG"
                     /locus_tag="VF954_03000"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX9530078.1"
                     /translation="MLFPEGSVALVTGASRGIGRATALELSRQGATVVVNFSRSQEQA
                     EEVVGEIERAGGSAVPWQADVADEDQVRAMMRMVKSRFKRLDILVNNAGSTADGFVVM
                     MSTEKWDHVLRVNLRGTFLCTREALKLMVHRRAGSIVNISSVSGLIGTEGQANYAASK
                     GAVHAFTKTVAREGAGFGVRANAVAPGLVETDMIATIPPALLDTFMNTIPMKRVGTPE
                     EIASVVAFLASDGASYITGTVVSADGGLAP"
     gene            complement(768..1772)
                     /locus_tag="VF954_03005"
     CDS             complement(768..1772)
                     /locus_tag="VF954_03005"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX9530079.1"
                     /translation="MRAFLFPGQGTQRPGMGQRLCAAFAPAREVFAAASDVLGFSVQD
                     VCFGDRTALLASTRYAQPVVFTCSMASLAVLADAGLTPDAVAGHSVGEFAALVAAGVV
                     GFEDAIRSLAVRGALMQQTAPGAMAVVLGLPADDVVALRQKVSAPDAVVVGLYNGPTN
                     VVVSGARAAVEEISALAKAAGALRVIAMATDRAFHSPLMEPVVAAWDAHLEGVTFRPP
                     QLPVALCTTGRTAADPEEIRRATVAQLTSPVRWADVMDELVGLGMDTAVEVGESKTLC
                     GFVRMLDRGLGTETTQDVRWLDTVVREGYTVVGHGATAMERMARVDTMVRRGGFDAVS
                     "
     gene            2059..3087
                     /locus_tag="VF954_03010"
     CDS             2059..3087
                     /locus_tag="VF954_03010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9530080.1"
                     /translation="MRLGLNVMRPSIRFGQWGSDLSETAKIVSLAEDLGYHSVWTAEA
                     SGTDATSPLAWLAAQTTKIQLGTAIMQIAGRSPGTTAMTAATLDLLSGRRFLLGLGVS
                     GAAVVEAWHNQPYGRPLAKTREYVTIVKQILARRAPIDFHGEYYDIPYYGPGSTHLAD
                     PIKLMFRPPRTSVPIYIAAMGPKNVSLAFEVADGIIPAFYSPYNEDKFFGDIPKEKRK
                     SLDIAPFVYVCVGDDIGACRDRLRPGFAFWFGVMGSRRVNYYNEFAQRLGYEEAAAKI
                     AKLYLEGRPGEAALAVPDELIDEVALCGPPARIRDQLAVWSESSVGTMICSGVDRDTI
                     VLLAELAL"
     gene            complement(3103..3705)
                     /locus_tag="VF954_03015"
     CDS             complement(3103..3705)
                     /locus_tag="VF954_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX9530081.1"
                     /translation="MIRHVVPDGVVAVEGTEEDSGRAGGGRTASTLPDHRRREFAHGR
                     ACAAEALAALGCPDVEVPVGSKGEPIWPPGVVGSLAHRRGYAAAAVAPRSRLRGLGID
                     VEIDAALSPGAARRVCAPAELAAAEATGLAAASTVVFSAKESVYKAVYPLTRRRLTYG
                     DVTVRLDPDVAAFEVDGALRASGCFAIIGGRIFTAVPIGP"
     gene            complement(3702..5516)
                     /locus_tag="VF954_03020"
     CDS             complement(3702..5516)
                     /locus_tag="VF954_03020"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX9530082.1"
                     /translation="MPEPPVARGPDWMAGVRALAASVQRPVSEVRADAEVKLAQVATS
                     INPAVCAALAAASGVVHRLTFSNVRYDPEQVSRLAKVMHDRPVALLWTHRSYLDGLLV
                     RSILQAHGLPRTYTAVGANLGFWPLGPLGRFAGAIFVRRGERETPVERFALGEYIAFL
                     LSRGEHLDWAPEGTRSRTGKLMPVKTGLLRHVLAAADRDTVDGVALVPVAIVYDDLQE
                     LDELVAYGQGAKKQAEGLSWMARYATSWRPNRPDVHVRLGDPVLVGGSTDTDGLCRDP
                     NAAAARIARELNGLTPATAPALVLLALHGAPSWGMPVGAVVAEAGALAGYARRWGAEV
                     LIPDIGEPSAVEETVEQAVADLARRGLVLVEQSAAGTAARLAPRRALQASYYRNSVAH
                     HFLVGAVAELAFLVAPSATGAGSVAAHALRIRSLLAGEFTFVPHDDADFVAAVRRDLD
                     MRGADMTSVGRGRPQASRIASLSRMRPLLAHRTLRPFLEGYAVAADTVAAAAGDTAAY
                     AAGDTAFYTAADTAAGCTAEPLLVESAQRRCRQLVADGTLGAADATSWWVLRNGVRLA
                     AARRGHAPRLAAELWHLVDQTEVLASLAVRPQPVGETA"
     gene            complement(5538..>6565)
                     /locus_tag="VF954_03025"
     CDS             complement(5538..>6565)
                     /locus_tag="VF954_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530083.1"
                     /translation="INDSGTYLLRTPRQSELDHVDEVTPEVLMARADAAKIRVLDHRL
                     DCPREFPCYIGRNRYVSNLPGSTILVPVIDLTRQYINGLLYLLSQDDGHRPTPIDDWN
                     FYRKAGVGKWVKNGFLNPDLPLPLGYLGTFRMHVESDLLFQNLLLTIQAMGLGGWVHA
                     AFPDHILLGDPAFAKYGSGLGFRWAPAKRNLMRILRTPITPLPAWRANPVGLDGKLEA
                     YCPPYYPNMSAAVEAILREKYGPTGPYSDPALFDKVFKPGLAQQFLDEVPHYSDDVIE
                     CVKDVCNYIYDTYGRFPAHADAIHVPGLWVQAHHLDLKYYDHLYQGGYSSTQANHHDL
                     WHGNARD"
BASE COUNT          993 a         2334 c         2328 g          910 t
ORIGIN      
        1 tggggggctc gtcgagggtc gggctcggaa ggatctaggg ggccaggccg ccgtcggcgc
       61 tgacgacggt gccggtgatg tacgacgccc cgtccgaggc caggaaggcg acgacggagg
      121 cgatctcctc gggtgtcccg acccgcttca tggggatggt gttcatgaac gtgtcgagca
      181 gggccggggg gatggtggcg atcatgtcgg tctcgaccag gcccggggcc accgcgttgg
      241 cgcggacccc gaatcccgcc ccttcgcgcg cgaccgtctt ggtgaaggca tggacggcgc
      301 ccttcgacgc cgcgtagttg gcctggccct cggtgccgat cagcccgctg accgacgaga
      361 tgttcacgat gctgccggcc cggcggtgga ccatgagctt caacgcctcc cgcgtacaga
      421 ggaaggtgcc ccgcaggttg acccggagca cgtggtccca tttttccgtg ctcatcatca
      481 cgacgaagcc gtcggcggtc gatccggcgt tgttgacgag gatgtcgagg cgtttgaacc
      541 gggacttcac catccgcatc atcgcccgga cctggtcctc gtcggcgacg tcggcctgcc
      601 acgggaccgc ggagccaccg gcccgctcga tctcgccaac cacctcctcg gcctgctcct
      661 ggctgcggga gaagttgacg acgacggtcg caccctgcct ggacagctcc agagcggtcg
      721 cccggccgat gccccggctg gccccggtca cgagggcgac cgacccctca ggaaacagca
      781 tcgaaccccc cgcggcggac catggtgtcg actcgcgcca ttcgctccat ggcagtggcc
      841 ccatgaccga ccacggtgta gccctcccgg accaccgtgt cgagccatct cacgtcctgg
      901 gtggtctccg tgccgaggcc gcggtcgagc atgcgcacga acccgcacag cgtcttgctc
      961 tcgcccacct cgacggccgt gtccatcccg agcccgacga gctcgtccat gacgtcggcc
     1021 caccgcaccg ggctcgtcag ctgggcgacc gtcgcgcgcc tgatctcctc cgggtcggca
     1081 gcggtccggc cggtcgtgca gagcgcgacg ggcagctgcg gcggccggaa cgtcacgcct
     1141 tcgaggtggg cgtcccacgc ggccaccacc ggttccatca gcggggagtg aaacgcccgg
     1201 tcggtcgcca tggcgatgac ccggagcgcc ccggccgcct tggccagcgc cgagatctcc
     1261 tccacggccg cccgggcgcc cgagaccacc acgttggtgg gcccgttgta caggcccacc
     1321 acaacggcgt cgggcgcgga gaccttctgg cgcagggcca cgacgtcgtc ggcgggcagg
     1381 ccgagcacca ccgccatggc cccgggcgcg gtttgctgca tgagcgctcc gcggacggcg
     1441 agggaccgga tggcgtcctc gaagcccacg acgccggcgg cgaccagggc ggcgaactcg
     1501 cccacgctgt gcccggcaac ggcgtcgggc gtgaggccgg cgtcggccag caccgccagc
     1561 gacgccatgc tgcaggtgaa caccaccggt tgggcgtacc gggtgctggc caggagcgcg
     1621 gtccggtccc cgaagcacac gtcctgcacg ctgaagccaa gcacgtccga ggcggccgcg
     1681 aacacctcac gcgccggggc gaaggccgcg cagaggcgct gacccatccc cggtcgctgc
     1741 gtaccctgcc cagggaagag gaaggcgcgc attcccgacc tcaccgtccg gccccccggc
     1801 gcccggagag cgtgcggcca cggcgacacc tatcgcgacc cccgtcgccg ggtcgcgtcg
     1861 agatatgaaa gccggcggca acaaagtacg cggtttcata agatttcatc agatagaggt
     1921 catatccagt aaatccagca acgaccggcc gttcgcccgt ccgccttccg cccaaatcct
     1981 acccgcccgg gggcggctgc gaacacagct cgtgataagt tcgccatcac ctggggggcc
     2041 accagtaagg acggagttat gagactcggc ctgaatgtga tgagaccgtc gatccggttc
     2101 ggccagtggg gatcggacct ctcggagacg gcgaagatcg tgtcgctcgc agaggatctg
     2161 gggtaccact cggtctggac ggcagaggcg tcggggaccg acgccaccag cccgctcgcc
     2221 tggttggcgg cccagacgac gaagatccag ctcgggaccg cgatcatgca gatcgccggc
     2281 cggtcgcccg gcaccaccgc gatgacggcc gccaccttgg acctgctgtc gggccgccgc
     2341 ttcctgctcg ggctgggcgt gtccggggct gccgtcgtcg aggcctggca caaccagccg
     2401 tacggccggc cgctggccaa gacccgagag tacgtgacca tcgtcaagca gatcctggcc
     2461 cgccgcgcgc cgatcgactt ccacggcgag tactacgaca tcccgtacta cggtccgggg
     2521 agcacccacc tggccgatcc catcaagctc atgttccggc ctccgaggac ctccgtcccg
     2581 atctacatcg ccgccatggg ccccaagaac gtgtccctcg cgttcgaggt cgccgacggg
     2641 atcatccccg cgttctacag cccctacaac gaggacaagt tcttcggcga catcccgaag
     2701 gagaagcgca agtccctcga catcgccccg ttcgtgtacg tctgcgtcgg cgacgacatc
     2761 ggcgcgtgcc gcgaccggct ccgcccgggc ttcgcgttct ggttcggcgt gatgggcagc
     2821 agacgggtca actactacaa cgagttcgcc cagcggctcg gctacgagga ggccgcggcc
     2881 aagatcgcca agctgtacct ggaaggaagg ccgggagagg cggcgctggc ggtgcccgac
     2941 gagctgatcg acgaggtggc gctctgcggt cccccggcgc gcatccgcga ccaactggcg
     3001 gtgtggagcg aatcgagcgt cggcaccatg atctgctccg gcgtggaccg cgacacgatc
     3061 gtcctgctgg ccgagctggc gctgtgagcg ggacatcgcg cgtcaggggc cgatcgggac
     3121 ggccgtgaag atccggccac cgatgatcgc gaagcaaccc gacgcccgga gggcaccgtc
     3181 cacctcgaag gccgccacgt ccgggtcgag ccggacggtt acgtcgccgt aggtcagccg
     3241 ccggcgagtc agcgggtaca cggccttgta caccgactcc ttggcgctga acacgacggt
     3301 ggacgcggcg gccagccccg tcgcctcggc ggccgcgagc tcggccgggg cgcagacccg
     3361 gcgcgccgct cccggtgaga gggcggcatc gatctcgacg tcgatgccca gcccgcgcag
     3421 ccggctccgc ggggcgaccg cggcggcggc gtagccgcgc cgatgggcga ggctgcccac
     3481 caccccgggc ggccagatcg gttcaccctt ggagccgacc gggacctcga cgtcgggaca
     3541 gccgagggcg gcgagggcct cggccgcgca cgctctgcca tgggcgaact cgcgccgacg
     3601 gtggtcggga agcgtcgagg cggtgcgccc accgccggcc cggcccgagt cttcctcggt
     3661 gccttccacg gcgaccacgc cgtcgggtac gacgtggcgg atcacgccgt ctcaccgacc
     3721 ggctgcgggc gcaccgccag gctggccagc acctcggtct ggtccaccag gtgccacagc
     3781 tccgccgcca gcctcggcgc gtggccgcgc cgggccgccg ccagtcgcac gccgttgcgc
     3841 aggacccacc acgacgtggc gtcggccgcg cccagggtgc catcggcgac cagctggcgg
     3901 caccggcgct gggcgctctc cacgagcaac ggctccgcgg tgcagccggc tgcagtgtcg
     3961 gcggcggtgt agaaggcggt gtcgcccgct gcgtaggcgg cggtgtcccc cgcggcggcg
     4021 gcgacggtat cggccgccac cgcgtacccc tccaggaacg ggcgaagcgt ccggtgggcg
     4081 agaagtggcc gcatacgtga gagagaggcg atccgggacg cctgggggcg accccgcccc
     4141 acgctggtca tgtccgcgcc cctcatgtcg agatcgcgcc ggacggcagc cacgaagtcc
     4201 gcgtcgtcgt gggggacgaa ggtgaactcc ccggcaagca gcgaccggat acgcagggca
     4261 tgggccgcga ccgagccggc gccggtggcc gacggggcca cgaggaaggc cagctcggcg
     4321 accgcgccga ccaggaaatg gtgggcgacg gagttgcggt agtaggacgc ctggagggcc
     4381 cggcgagggg ccagcctggc cgcggttccg gccgccgact gctcgaccag cacgaggccg
     4441 cgccgggcca ggtcggcaac cgcctgctcc acggtctcct ccacggccga cggctcgccg
     4501 atgtcgggga tcagcacctc cgcgccccat cgccgggcgt agcccgccag cgcgcccgcc
     4561 tccgcgacga ccgccccgac gggcattccc cacgaggggg ctccgtggag ggccagcagc
     4621 accagggccg gggcggtggc gggggtcagc ccgttgagct cccgggcgat gcgcgcggcc
     4681 gcggcgttcg ggtcgcggca caacccgtcc gtgtcggtgc tcccgcccac caggaccgga
     4741 tcaccgagac gcacatgcac gtcgggccga ttcggccgcc agctcgtcgc gtacctcgcc
     4801 atccacgaca acccctccgc ctgcttcttg gcgccctgcc cgtacgcgac cagctcgtcc
     4861 agctcctgga ggtcgtcgta cacgatggcg acggggacga gcgccacccc gtccaccgtg
     4921 tcacggtcgg cggcggccag cacatggcgc agcagccctg tcttgacggg catcaacttg
     4981 cccgtccggc tgcgcgtgcc ctcgggagcc cagtccaggt gctcgccccg cgacagcagg
     5041 aaggcgatgt actcaccgag cgcgaaccgc tcgacgggtg tctcccgctc ccctcgccgg
     5101 acgaagatgg cgcctgcgaa gcggccgagc ggcccgagag gccagaagcc caggttggcg
     5161 ccgacggcgg tataggtgcg gggcaggccg tgggcttgga ggatcgagcg cacgagaagc
     5221 ccgtcgagat agctgcggtg cgtccagagc aacgcaaccg gccggtcgtg catgaccttg
     5281 gccaggcggc tcacctgctc cgggtcatag cgcacgttgc tgaaggtcag gcggtgcacc
     5341 acgcccgacg cggccgccag ggcggcgcac accgccgggt tgatgctcgt ggccacctgg
     5401 gcgagcttca cctcggcatc ggctcgcacc tcggagaccg gccgctgcac gctcgccgcg
     5461 agcgcccgga cgcccgccat ccagtccggg ccccgggcga ccggcggctc tggcatcgcc
     5521 ggccctgtca tcgcggctca gtcacgggcg ttcccatgcc acaggtcgtg gtggttggcc
     5581 tgggtgctcg agtacccccc ctggtagagg tggtcgtagt acttcaggtc gaggtgatgc
     5641 gcctgcaccc acaggcccgg gacgtggatg gcatcggcgt gggcggggaa ccggccgtag
     5701 gtgtcgtaga tgtagttgca cacgtccttg acgcactcga tgacgtcgtc gctgtaatgg
     5761 ggcacctcgt cgaggaactg ctgggcgagc ccgggcttga agaccttgtc gaagagcgcc
     5821 gggtccgagt aggggccggt tggcccgtac ttctcgcgca ggatggcctc gaccgcggct
     5881 gacatgttgg ggtagtaggg cgggcagtag gcctcgagct tcccgtccag gcccaccggg
     5941 ttggcccgcc acgccgggag cggggtgatc ggcgtcctca ggatccgcat caggttccgc
     6001 ttcgcggggg cccagcggaa ccccagcccg gacccgtact tggcgaaggc gggatcgccg
     6061 agcaggatgt ggtcgggaaa cgcggcgtgg acccagccgc cgaggcccat ggcctggatg
     6121 gtcagcagga ggttctggaa gaggagatcc gactcgacgt gcatcctgaa ggtgccgaga
     6181 tagccgagcg gcagcggcag gtccgggttg aggaacccgt tcttgaccca cttgccgacg
     6241 ccggccttgc gatagaagtt ccagtcgtct atgggcgtgg gccggtgccc gtcgtcctgg
     6301 gacagcaggt agagcagccc gtttatgtac tggcgggtga gatcgatgac cggcaccagg
     6361 atcgtggagc cgggcaggtt ggagacgtag cggttccgcc cgatgtagca ggggaactcc
     6421 cgcgggcagt cgaggcggtg gtcgagcacc cggatcttcg ccgcgtcggc ccgcgccatc
     6481 aggacctcgg gcgtcacctc gtcgacatgg tccagctcgc tctgacgggg cgtccgaagc
     6541 aggtacgtcc cgctgtcgtt gatca
//
LOCUS       DASWGG010000166         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3066637, whole genome shotgun sequence.
ACCESSION   DASWGG010000166 DASWGG010000000
VERSION     DASWGG010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3066637"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VF954_03030"
     CDS             complement(<1..601)
                     /locus_tag="VF954_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9530084.1"
                     /translation="MADVLVARGLRKAYRQSLAVDGVDLTIGVGERVALLGPNGAGKT
                     TTLLMLLGAVSPDSGTIELLGHPLPRERSEAMEGVGFAAGYLPLPERLRVGEVLRIFA
                     DLYGVPDPQRAVVAGMERFGVAHLAKRMASELSSGQRTLVGIVKATMHRPQLLVLDEP
                     TASLDPDVAERVRRGVESLWAEDGTALLVTSHNMTDVERV"
     gene            857..2233
                     /locus_tag="VF954_03035"
     CDS             857..2233
                     /locus_tag="VF954_03035"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX9530085.1"
                     /translation="MAENELPGTPNWRIANLGSEHDIEGWADAASVLVGEKVRLHVST
                     PAASYTVHAIRIGWYGGADGREVWSSPPVPGAVQPAATVIEPRHTAVTDWPVSLQVDT
                     TGWPAGDYLLRLDSSAGPQRYVPLQVRRPSAAGTVVVVSADTTWQAYNRYGGYSLYEG
                     PNGSFATRARAVSFDRPYSITTGQGASEYMENMRPLVTLVEQLGLDADYVSDVDLEAD
                     PHVVDGAAAVVMTGHDEYWSLAMRQALLDGRGRGTNLAFLGANSGYRHIRFEPSTAGA
                     ARRIEVCYKVAAEDPLYGKDNADVTDQWRNPPDPRPESAITGVFYQSNPVQADMVVVD
                     PAGWMLQGTGAAVGTRVPSVVAPEYDRVDLSVPTPRPIEIVTHSPLTVHGSPDFSDSA
                     YYTTDSGAGAFATGTIAWINSLQGASGPAAAAFTTQVTKNVLLAFAAGPAGRLHPAQD
                     NVATFPRV"
BASE COUNT          380 a          943 c          860 g          352 t
ORIGIN      
        1 agacgcgctc gacgtcggtc atattgtggg aggtgacgag gagggcggtg ccgtcctccg
       61 cccacaatga ctccacgccc cggcgcaccc gctcggcgac atccggatcg agggacgcgg
      121 tgggctcgtc gaggacgagg agctggggcc ggtgcatggt cgccttcacg atgccgacga
      181 gggtgcgctg gcccgaggac agctcgctgg ccatccgctt ggccagatgg gcgaccccga
      241 agcgctccat gcccgcgacg acggcgcgct gcgggtcggg caccccgtag aggtcggcga
      301 agatcctcag cacctcgccc acccgcagcc gctcgggcag gggcaggtat ccggccgcga
      361 agccgacgcc ctccatggcc tcgctgcgct cacgcggtag cgggtggccg agcagctcga
      421 tggtccccga gtccggcgag accgccccga ggagcatgag cagcgtggtc gtcttgcccg
      481 ccccgttggg accgagcagc gcgacgcgct cgccgacgcc gatggtgaga tcgacgccgt
      541 ccaccgccag cgactggcgg taggccttgc gcaggccgcg agctaccagg acatcggcca
      601 cgtcgttgac tgtgccagag accgtgctcg gcttgcgttc cattttctcc agaacccgtc
      661 gagctacgat gcccggagca tgcgcatgac gcccgccggt cggagctggt gtcagtgcgt
      721 cggcgggttc gcggtcgtcg ccctcgtgtt ggccggctgc tccaacgccg ccggcactca
      781 cccccggagc ccggcacccc ctacatcagc ggggacgaca tctgccacct cgacgacgac
      841 accacctccc gccacggtgg ccgagaacga gcttccgggc acgcccaact ggcgcatcgc
      901 caacctgggc tccgagcacg acatcgaggg ttgggccgat gccgccagcg tgctcgttgg
      961 ggagaaggtc aggcttcatg tctccacgcc ggctgcgagc tacacggtcc acgccatccg
     1021 catcgggtgg tacggcgggg ccgacggccg cgaggtctgg tcctcgccgc cggtccccgg
     1081 ggccgtgcaa ccggcggcca ccgtgatcga gccgcgccac accgcggtca ccgactggcc
     1141 ggtctccctg caagtggaca cgacgggctg gcctgccggg gactacctgc tcaggctcga
     1201 ctcgtcggcc ggcccccagc gctacgtccc cctgcaggtc cgccggccat cggcggcggg
     1261 cacggtggtg gtcgtcagcg ccgacacgac ttggcaggcc tacaaccgct acggcggtta
     1321 cagcctctac gagggcccca acggcagctt cgccacccgg gcgagagccg tgtccttcga
     1381 ccgcccgtac tcgataacca ccggccaggg ggccagcgag tacatggaga acatgcgccc
     1441 gctcgtcacc ctggtagagc agctgggact ggacgccgac tacgtcagcg acgtcgatct
     1501 ggaggccgac ccgcacgtcg tcgacggggc ggcggcggtg gtcatgacgg gccacgacga
     1561 gtactggtcg ctggcaatgc gccaggcgct tctcgacggg cgcggtcgcg gcacgaacct
     1621 cgcgtttctc ggcgccaaca gcggctaccg ccacatccgg ttcgagccgt cgacggcagg
     1681 cgccgcccgg cggatcgagg tttgctacaa ggtggccgcc gaggaccccc tatatggcaa
     1741 ggacaacgcc gacgtcaccg atcagtggcg gaaccccccg gacccgagac ccgagagcgc
     1801 gatcacgggc gtcttctacc agagcaaccc ggttcaggcc gacatggtcg tcgtggaccc
     1861 cgcggggtgg atgctccagg gcaccggtgc ggcggtcggg acccgcgtgc cctccgtggt
     1921 cgcgcccgag tacgaccggg tcgatctctc cgtgccgacc cctcgcccca tcgagatcgt
     1981 cacgcactcg ccgctcacgg tccacggctc gcccgacttc tccgactcgg cttactacac
     2041 caccgacagc ggggcgggag cctttgccac ggggacgatc gcctggatca actccctgca
     2101 gggcgcgagc ggaccggcag cggccgcctt caccacccag gtaaccaaga acgtcctgct
     2161 cgcgttcgcc gccggtcccg ccggccggct acatcccgcc caggacaacg tcgccacctt
     2221 cccccgggtc tgacggggcc gacctcgacc ccacctcgac ccgacctcca cccgatgtcg
     2281 ggccgcagtc gagatgcact aacgtctccc gccgttggga tgacccggcg gagggaacat
     2341 ggcacagctg cgcggcaggg aacctaggca ccggaacacg gcgccgttgg tcctcggcat
     2401 gctggcggcc gggctcgcgg ccgctgcgat ggcgctcgtc ctcgccgcgg tcaccgcccg
     2461 gggcgcggcg gcgtcggtcg ctccctccca cccggtcagc ggctactgga tgggggcctc
     2521 ggacggaggc atctt
//
LOCUS       DASWGG010000167         2956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1137911, whole genome shotgun sequence.
ACCESSION   DASWGG010000167 DASWGG010000000
VERSION     DASWGG010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137911"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VF954_03040"
     CDS             <1..265
                     /locus_tag="VF954_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530086.1"
                     /translation="SAEALDAVMAAGGSLVALGVLVVVLDSLRALFGRGDHAADDPYD
                     GLTLEWATTCPPPPANFDAIPEIRSAQPLADLRVAPGASGTAP"
     gene            265..852
                     /locus_tag="VF954_03045"
     CDS             265..852
                     /locus_tag="VF954_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX9530087.1"
                     /translation="MTAPTLALPPGGRSQPRNTPTFGALVLVVAGAMAVGTLSAVFMA
                     LSFRAHQPPSGVRLDEYPAVVAGLTLILSSVTITWALYGLRLGERRQAMAALTATLLF
                     GLADVSLVWFILAQAGFGVASSPFATLFYALVGTGGLLVAMGLVMVAGCIGKVAGNQL
                     NAFEGEGLRAVLWYWHFAVGAWIVISLTIYPVVKL"
     gene            860..1867
                     /locus_tag="VF954_03050"
     CDS             860..1867
                     /locus_tag="VF954_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530088.1"
                     /translation="MSAASKLFAAVSGTALLLAVAYAMSAQGSVPITLLVALASAAAV
                     AVVVTTAVGGRDLAPAVPLDAPPPESRSITAGAPARPSMWPPVVAAGIGLVAVGAAVG
                     MGWLVAGLVVALAGTAGWYGRTWREHPSWTPRVSERVQNRLVLPAGLFWAILLCVAVV
                     AIAVSRILLAVPEKASVAVALGMAVILLVAFFVIASQSRTRRSVVATLVVVTALALGG
                     AGLASGLKGERSYDLVAPPPSAQLLVAHNTAFAQTQLNGSVGQKVLVDFRNLDKGTYH
                     NFSIYTQRSDGSQGAPVFNGKPINGGTILYSLVAPEGPGSYIFACDFHSNMRGTFTVA
                     S"
     gene            1864..2913
                     /gene="coxB"
                     /locus_tag="VF954_03055"
     CDS             1864..2913
                     /gene="coxB"
                     /locus_tag="VF954_03055"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX9530089.1"
                     /translation="MTRQLRNRRRRVLGAVLTLLVLALTSCGHTNLPQDALDPRGHYS
                     RTTYNLIVPVFVVAGVVFVLVQGLILYSVIRFRRRSEDEAPKQVHGNTRLEITWTVLP
                     FLTLLVIAVFTVVGIVQLSHRPKGPNVVEVNVTGHQWWWELNYPGYGVTTANELHVPT
                     HQPVDIALTSVDVIHNFWPPKLAGKIYAIPNHLNHMTLEADEPGVYYGQCAEFCGLSH
                     ANMRFRVVAQTPEDFQAWAAANRTDSPANASYALPDPETQADANAGAVLFRTKGCAGC
                     HSITGYSAGKVGPNLTHLDQRTVFAGALFDLNDNNLRNWLRDPPGVKPGSKMPNLNLS
                     EDDITKLIAYLDTLK"
BASE COUNT          388 a         1153 c          955 g          460 t
ORIGIN      
        1 ctcggccgag gccctcgacg ccgtcatggc ggccgggggc tccctggtgg ccctcggcgt
       61 gctggtcgtc gtgctcgaca gcctgcgcgc cctgttcggc cggggtgacc atgccgccga
      121 cgacccctac gacggcctca ccctggagtg ggccacgacc tgcccgccac cgcccgccaa
      181 cttcgacgcc atcccggaga tccgctcggc ccagccgctc gccgacctgc gggtcgcgcc
      241 cggcgcttcc gggacggccc cctgatgacc gcccccaccc tcgccctgcc gcccggcggg
      301 cgcagccagc cccgcaacac gccgaccttc ggcgcgctgg tcctcgtcgt ggccggcgcc
      361 atggccgtcg gcaccctcag cgcggtgttc atggcgctga gcttccgcgc ccaccagccc
      421 ccgtccggcg tccgcctcga cgagtacccc gccgtcgtgg ccggcctcac gctcatcctc
      481 tcgtccgtga cgatcacgtg ggccctgtac gggctgcgcc tcggcgagcg gcgccaggcg
      541 atggcggcgt tgaccgccac gctgctgttc ggcctggccg atgtgagcct ggtctggttc
      601 atcctggccc aggccggctt cggcgtggcc agctcgccct tcgccaccct cttctacgcc
      661 ctcgtaggca ctggcggctt gctcgtggcg atgggcctgg tgatggtggc gggctgcatc
      721 ggcaaggtgg ccggcaacca gctcaacgcc ttcgagggcg agggcctccg ggccgtgctg
      781 tggtactggc acttcgccgt cggcgcatgg atcgtcatct ccctgaccat ctacccggtc
      841 gtcaagctgt gacccggcga tgagcgccgc gtccaagctc ttcgccgccg tgtccgggac
      901 ggccctgctg ctggcggtcg cctacgccat gtcggcccag gggtcggtgc cgatcaccct
      961 gctcgtcgcc ctggcctcgg cggcggccgt cgcagtggtg gtgacgaccg cggtcggcgg
     1021 acgcgacctg gccccggccg tgcccctcga cgccccgccg cccgagagcc gctccatcac
     1081 cgccggggcc ccggcccgcc cgagcatgtg gccccccgtc gtcgccgcgg ggatcggcct
     1141 ggtcgcggtc ggcgccgccg tcgggatggg ctggctggtc gccgggctcg tcgtggccct
     1201 ggccggcacg gccggctggt atggccggac ctggcgggag cacccgagct ggacgccgcg
     1261 tgtcagcgag cgggtgcaga accggctggt gctgcccgcc gggctgttct gggccatcct
     1321 gctgtgcgtc gcagtcgtcg ccatcgccgt gtcccgcatc ctgctggccg tgcccgagaa
     1381 ggcgtccgtg gccgtcgccc tcgggatggc cgtgatcctg ctggtcgcct tcttcgtgat
     1441 cgcctcgcaa tcgcgcaccc ggcgctcggt ggtcgccacc ctcgtggtgg tcacagccct
     1501 cgccctcggc ggcgccggcc tggcgagcgg gctgaagggc gagcgcagct acgacctggt
     1561 cgccccgccg ccgtcggccc agctgctggt cgcccacaac accgccttcg cccagacaca
     1621 gctcaacggc tcggtcgggc agaaggtgct ggtcgacttc cgcaacctcg acaagggcac
     1681 ctaccacaac ttctccatct acacgcagcg atccgacggg agccaggggg cgccggtgtt
     1741 caacggcaag ccgatcaacg gcggcacgat cctctacagc ctcgtggctc cggagggtcc
     1801 tgggtcatac atcttcgcct gtgacttcca ctccaacatg cggggcacgt tcacggtggc
     1861 atcgtgacgc ggcagctccg caaccggcgc cgccgggtcc taggggccgt cctgacgctg
     1921 ctcgtgctgg ccctgacgtc gtgcggccac accaacctgc cccaggacgc cctcgacccg
     1981 cgcggccact actcgcgtac gacctacaac ctgatcgtgc cggtgttcgt ggtggccggg
     2041 gtcgtgttcg tcctcgtcca gggcctcatc ctctacagcg tgatccggtt ccggaggcgg
     2101 tcggaggacg aggcacccaa gcaggtccac ggcaacaccc gcctggagat cacctggacg
     2161 gtgctgccct tcctaaccct gctcgtcatc gccgtcttca cggtcgtcgg catcgtccag
     2221 ctgtcgcacc gccccaaggg ccccaacgtg gtcgaggtca acgtgaccgg ccaccagtgg
     2281 tggtgggagc tcaactaccc gggctacggc gtgaccacgg ccaacgagct gcacgtcccg
     2341 acccaccagc cggtcgacat cgccttgacg tcggtcgacg tcatccacaa cttctggccc
     2401 cccaagctgg cgggcaagat ctacgccatc cccaaccacc tcaaccacat gaccctcgag
     2461 gccgacgagc ccggcgtgta ctacgggcag tgcgcggagt tctgcgggct ctcccacgcc
     2521 aacatgcgct tccgggtggt ggcccagacg cccgaggact tccaggcctg ggccgcggcc
     2581 aaccgcacgg actcgccggc caacgcgtcc tacgccctgc ccgacccgga gacccaggcc
     2641 gacgccaacg ccggggccgt gctgttccgc accaagggct gcgcaggctg ccactcgatc
     2701 accgggtaca gtgccggcaa agtgggaccc aacctcaccc acctggacca gcgcacggtg
     2761 ttcgccgggg ccctgttcga cctgaacgac aacaacctgc gcaactggct gcgggacccc
     2821 ccgggggtca agccgggctc gaagatgccc aacctcaacc tgtccgagga cgacatcacc
     2881 aagctgatcg cctacctgga caccctcaag tgaccggaca cctcgtcccc ttcgcgccca
     2941 ccggaggctg ctgatg
//
LOCUS       DASWGG010000168         5073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2014548, whole genome shotgun sequence.
ACCESSION   DASWGG010000168 DASWGG010000000
VERSION     DASWGG010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014548"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VF954_03060"
     CDS             complement(<1..369)
                     /locus_tag="VF954_03060"
                     /inference="COORDINATES: protein motif:HMM:NF036723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530090.1"
                     /translation="MLPDYAEAEQAIGLDWYGADPNLRFVLDHLLPDPDDRAFAEEHV
                     GAFGPLVGQVIAPRAEVTDKHGPQLHGYDRWGVEVNEVVHHPTWTANKSDLIAAGFVG
                     LPHRADRPVPAVVTAALAYLV"
     gene            complement(416..2272)
                     /locus_tag="VF954_03065"
     CDS             complement(416..2272)
                     /locus_tag="VF954_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX9530091.1"
                     /translation="MTDQAVDPGVSSATELDRARAERAAERAGAVVEAAAPPTGGHLP
                     IVVSGRNVGRFDSRPKLGRVLVDQGMVTEDEIEAALQTQRETGERIGRILVEEGIITS
                     VQLTKALAEAAGVGFVDLDDILLDPSVARALPETFARRAKAVAIGTEGEKIVVAMVNP
                     SDVFAVDDMRTVTKSQIRPVMADEGQILRALERVWRSGAEAEAMLQLASDEAEVDDVA
                     LPGGDHAAEDAPVVQFVNELLTRAVHDRASDIHLEPTGQHLRVRFRVDGVVRDVMTVP
                     RQIQGAVVSRLKIMADINIAERRKPQDGRVTMSLDERAVDIRVVTLPTAFGEAVVMRL
                     LDRSAAMRPVADLGFMPESLAAYQAAYTEPYGAILVTGPTGSGKSTTLYATLHELNDP
                     ARTIVTVEDPIEYRLAGIKQVQLNAKAGLTFASALRSILRADPDVVLVGEIRDEETAR
                     IAIEAALTGHKVLSSLHTNSAAATPTRLVEMGVEPYLVISSLGAVLAQRLVRQLCKCR
                     VPFEPKQAELEAAGWPEGFRESELVPSFCRAHGCEHCGGTGYRGRFAVQEVMTMTPAI
                     AHLVLEHASADDLAKQALAEGMISMRLDGLRKAAGGLTTIEEILRVVPRASH"
     gene            2399..3400
                     /locus_tag="VF954_03070"
     CDS             2399..3400
                     /locus_tag="VF954_03070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9530092.1"
                     /translation="MDLARTPVGVTSAPLQALGLPLTLDVASRAAELGYRSFWVAEAS
                     GPEAMSTLGAVSLVAPDLDLGTGVLALQLRTPPLTAMAAASLQALAPDRDILLGIGVS
                     SPVVAGRWHGAPFGDRPLAQVREFVALLRACLSGETVDFTGDFYHVSRFRLGVRLGER
                     RPKIVIGALNQGMLRLAGEIGDGVLLNYVPAAHVPWSVEQVRKGGDAVIYAYVHVGVT
                     ERDEQLDQARRDLFSYAVVDAYARNFARAGFGASVDRVRQAHAARDRDRAIAAVTDAF
                     VDAIDIMGDREQVRAAVEAYVAAGVDVPVVFPLPWGEDRRAVVEATLSAVAPRPRQG"
     gene            3414..4160
                     /locus_tag="VF954_03075"
     CDS             3414..4160
                     /locus_tag="VF954_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX9530093.1"
                     /translation="MCGRFVAATPPSALAERFQAELAPELAGDGELGPRFNVAPTLPV
                     YAVAASRRTGARRLGAFRWGLVPSWAADPSVGSRMINARVETVATRPAFRPALLRRRC
                     IIPADAFYEWQRRGGRTEGHTKQTKGHTRQPFAIRRRDGGLLPFAGLWEMWRAEDAPH
                     GSPLLRTCTIITTPANDAVASLHDRMPAILAPGAWDEWLDPAACDLSRLLRLLQPASP
                     ADFAVYPVSTEVNNARNEGAQLVLPIEPGG"
     gene            complement(4157..4882)
                     /locus_tag="VF954_03080"
     CDS             complement(4157..4882)
                     /locus_tag="VF954_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX9530094.1"
                     /translation="MEPATYLAAIRRESAAFAAAAGRDLTAPVPSCPGWDVAELVRHL
                     GRVQHWVAGGVRAGSRERFARVAPPPRDEVVPWFEAGAADLVAVLEEAGPDRAVWNFI
                     LEDGRVATTGWWFRRQAQEAAMHRWDAEAATGAPPVLEPELAADGVDEFLHIVLPHAP
                     GIGVELVGSLHLHRTDGEGEWMVRFLPAGMEVSREHGKGDTAVRGPASGLLLWLSNRT
                     GSDGLEVFGDRQLVERWPQLVRF"
BASE COUNT          656 a         1855 c         1820 g          742 t
ORIGIN      
        1 caccaggtac gccagagcgg ccgtcaccac cgcgggcacc ggccggtcgg cccgatgcgg
       61 caggcccacg aacccggcgg cgatgaggtc ggacttgttt gccgtccacg tcgggtggtg
      121 cacgacctcg ttcacctcga cgccccaccg gtcgtagccg tgaagttggg gcccgtgctt
      181 gtccgtgacc tcggcgcgag gggcgatgac ctggcccacg agcgggccga aggcgcccac
      241 gtgctcctcg gcgaatgcac ggtcgtcggg gtcgggcagg aggtggtcga ggacgaatcg
      301 caggttgggg tcggccccgt accagtccag cccgatggcc tgctcggctt ctgcgtagtc
      361 cggcagcatc ggtgccgacg ctactgccgc cgactgccga cgcacgccgg ggccctcagt
      421 ggctggcgcg gggcacgacc cggaggatct cctcgatggt ggtcagcccg ccggccgcct
      481 tgcggaggcc gtcgaggcgc atcgagatca tgccctcggc cagcgcctgc ttggccaggt
      541 cgtctgccga ggcgtgctcg agcaccaggt gggcgatcgc aggcgtcatg gtcatgacct
      601 cctgcacggc gaagcggcct cggtatccgg tgccgccgca gtgctcgcag ccgtgggccc
      661 ggcagaagct gggcacgagc tccgactcgc ggaagccctc gggccatccg gccgcctcga
      721 gctcggcctg cttgggctcg aacggaaccc ggcacttgca cagctggcgc acgagccgct
      781 gggcgagcac cgccccgagc gacgagatca ccaggtaggg ctccacgccc atctccacca
      841 ggcgggtcgg agtggccgcg gccgagttgg tgtgcagcga cgaaaggacc ttgtggccgg
      901 tgagggcagc ctcgatggct atgcgggccg tctcctcgtc ccggatctcg ccgacgagca
      961 cgacgtccgg gtcggcgcgc aggatcgacc gcaacgcgct ggcaaaggtc aggccggcct
     1021 tggcgttgag ctggacctgc ttgatcccgg cgaggcggta ctcgatcggg tcctcgacgg
     1081 tgacgatggt gcgagcgggg tcgttgagct cgtgcagggt ggcgtacagg gtcgtcgact
     1141 tcccggagcc cgtcgggccg gtgaccagga tggcgccgta cggctcggtg taggcggcct
     1201 ggtaggcggc caggctctcg ggcatgaagc cgagatcggc cacgggccgc atggcggctg
     1261 accggtcgag cagccgcatc acgacggctt cgccgaacgc cgtcggcagg gtgaccaccc
     1321 ggatgtccac ggcccgctcg tcgaggctca tcgtcacccg gccgtcctgg ggcttgcggc
     1381 gctcggcgat gttgatgtcg gccatgatct tgagccggga gaccaccgcg ccctggatct
     1441 ggcgggggac ggtcatcacg tcccggacca ccccgtcgac ccggaagcgg acccgcaggt
     1501 gctggccggt cggctcgagg tggatgtccg aggcccggtc gtgcaccgcc cgggtcagca
     1561 gctcgttgac gaactgcacg accggcgcat cctcggcggc gtggtcgccg cccgggaggg
     1621 cgacgtcgtc gacctcggcc tcgtcgctgg ccagctggag catggcctcg gcctcggcgc
     1681 ccgagcgcca gacccgctcc agggcccgca ggatctggcc ctcgtcggcc atgaccgggc
     1741 gtatctgcga cttggtcacg gtccgcatgt cgtcgacggc gaagacgtcg gacgggttca
     1801 ccatggcgac gacgatcttt tcgccttcgg tgccgatggc caccgccttg gcccggcggg
     1861 cgaaggtctc cggcagcgcc cgcgccaccg aggggtcgag caggatgtcg tcaagatcga
     1921 cgaacccgac gcccgccgcc tccgccaacg ccttggtgag ctggacgctg gtgatgatgc
     1981 cctcttcgac caggatccgc ccgatgcgct cgccggtctc gcgctgggtc tgaagggcgg
     2041 cctcgatctc gtcctctgtg accatgccct ggtcgacgag cacccgaccg agtttgggcc
     2101 ggctgtcgaa gcggcccacg ttccgccccg acacgacgat gggcaggtgg cctccggtgg
     2161 gtggcgcggc agcctcgacc acggccccgg cccgctcggc ggcccgttcg gcgcgggcgc
     2221 ggtccagctc ggtggccgag ctcacccctg ggtccacggc ttggtcggtc atgtgctcgg
     2281 gctcctcgag cacgaggacg agaacggtcc tgcggtggcc ctcacctgta cctaatcggc
     2341 cgtttggcac caagcctgta gcgggccccg gctcccgccc ggcgggtagg gtcggtccgt
     2401 ggacctcgcc cgcaccccgg tcggcgtcac ctccgccccc ctccaggccc tggggcttcc
     2461 gctgaccctc gacgtggcca gccgggcggc ggagctcgga taccgctcct tctgggtcgc
     2521 cgaggcgagc ggaccggagg ccatgagcac cctgggcgcc gtgtccctcg tcgctcccga
     2581 cctcgacctg ggcacggggg tcctggcgct gcagctgcgc acaccgccgc tgacagccat
     2641 ggccgcagcc agcctccagg ctctcgcgcc cgaccgcgac atcctgctcg gcatcggggt
     2701 ctcctcaccg gtggtcgccg gccgttggca cggtgcacct ttcggggatc gcccgctggc
     2761 gcaggtgcgg gagttcgtgg cgctgctgcg tgcgtgcctg tccggtgaga cggtcgattt
     2821 caccggcgac ttctaccacg tgtccaggtt ccgcctcggg gtgcgtctgg gcgagcggag
     2881 gcccaagatc gtcatcggcg cgctcaacca gggcatgctc cggctggcgg gcgagatcgg
     2941 cgacggcgtt ctgttgaact acgtgcccgc cgcccacgtc ccgtggtccg tcgagcaggt
     3001 gcggaaaggt ggcgacgccg tcatctacgc ctacgtccat gtgggggtca ccgagcggga
     3061 cgagcagctg gaccaggcgc ggcgcgacct gttctcgtac gcggtggtgg acgcgtacgc
     3121 ccgcaacttc gcccgcgccg ggtttggcgc atccgtcgac cgcgtgcggc aggcccatgc
     3181 cgccagggat cgcgaccggg cgatcgcggc ggtcaccgac gcgttcgtcg acgccatcga
     3241 catcatgggc gaccgggagc aggtgcgagc cgccgtcgag gcctatgtcg ccgccggcgt
     3301 agacgtgccg gtcgtgttcc cgttgccgtg gggcgaagac cgccgggcgg tcgtggaggc
     3361 caccctgagc gccgtcgcgc cccgtccgcg gcagggctag aggcggcgcg ccaatgtgcg
     3421 ggcggttcgt ggctgcgacg cccccgtcgg cgctggccga gcgctttcag gccgagctgg
     3481 caccggagct ggccggggat ggcgagctgg gcccgcgctt caacgtggcg ccgacgctgc
     3541 ccgtgtacgc ggtggccgcc tctcgccgta ctggcgcccg ccggctgggc gcgttccggt
     3601 ggggcctggt cccgtcgtgg gccgccgacc cctccgtcgg cagcaggatg atcaacgctc
     3661 gcgtcgagac ggtcgcgacc aggcccgcct ttcgaccggc cctcctccgg cgtcgctgca
     3721 tcatcccggc cgacgccttc tacgagtggc aacggcgggg tgggcgcacc gaggggcata
     3781 ccaagcagac caaggggcat accaggcagc ccttcgccat tcgccggcga gacggagggc
     3841 tcctcccctt tgccggcctg tgggagatgt ggcgcgccga agacgcgccc cacggctcgc
     3901 cccttctgcg gacctgcacc atcatcacca cgccggccaa cgacgccgtc gcgtcgctgc
     3961 acgaccgcat gccggccatc ctggcgcccg gagcctggga cgaatggctg gacccggccg
     4021 cctgcgacct gtcgcggctc ctccgcctgc tccaaccggc atcgcccgcg gacttcgccg
     4081 tctatccggt cagcacggag gtcaacaacg ctcgcaacga gggggcacag ctggtcctgc
     4141 cgatcgagcc cgggggctag aacctcacca gctggggcca gcgctcgacg agctgtcggt
     4201 ccccgaagac ctccagtccg tcgcttccgg tccggttgga cagccagagc aacagtcccg
     4261 acgccgggcc tcgcacggcc gtatcgccct tcccgtgctc gcgggacacc tccatcccgg
     4321 ctggcagaaa gcgcaccatc cactctccct cgccgtctgt ccggtgaagg tggaggctgc
     4381 cgaccagctc gacgccgatg cccggagcat gggggagcac gatgtgcagg aactcgtcca
     4441 caccgtctgc ggccagctcc ggctccagga ccgggggcgc cccggtggcg gcctcggcgt
     4501 cccagcggtg catggccgct tcctgggcct ggcgccggaa ccaccagccc gtggtcgcca
     4561 ccctgccgtc ctcgaggatg aagttccaca cggcccggtc ggggccggcc tcctccagca
     4621 cggcgacgag gtcggccgcg cccgcctcga accacgggac cacctcgtct cggggcggtg
     4681 gcgccacgcg ggcgaagcgc tcccgcgagc cggcccgaac cccgcccgcc acccaatgct
     4741 ggacccgccc caggtgccgg accagctcgg cgacgtccca gccggggcac gacggcaccg
     4801 gggccgtgag gtctcgcccc gcggcagcgg cgaaagccgc gctctcccgt cggatggccg
     4861 cgaggtaagt ggcgggctcc atgggtcggc attctcgcac gccctcgccc gccgccctgc
     4921 tagtcgagct ggaggaccgc gtcgtgtcgt gcggcgcgca ccgcgcccgc cacgcccgga
     4981 ccgtcgaggc cgagatcggc caggagctgg ttggccttgc cctgcgggat gtacgccgcc
     5041 ggggtcccga gcacgagcac cggaggggcc tgg
//
LOCUS       DASWGG010000169         9035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2745571, whole genome shotgun sequence.
ACCESSION   DASWGG010000169 DASWGG010000000
VERSION     DASWGG010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9035
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2745571"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VF954_03085"
     CDS             <1..392
                     /locus_tag="VF954_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530095.1"
                     /translation="SLDVVVVSPPGRCRPADARRLAARARDRHGVLVVVEEVPVGRTG
                     RVGRWPESVDLQLEAGASVWHGLDRGDGTLACREVTVRSFGRRSGGRERSTRLWLPAA
                     DGGPGLIGGEPRTGSGANGPAAGTLAG"
     gene            393..1931
                     /locus_tag="VF954_03090"
     CDS             393..1931
                     /locus_tag="VF954_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530096.1"
                     /translation="MAVRTLALWCPDWPVTAAGFGPNDPVAVLDAGRVVAAAGAARGA
                     GVRAGLRRREAESRCSGLVVLTRDVAAEARAFEAVVAAVATLTPRVEVTRPGLLALDA
                     RGPARYFGGEEMLCQRVAVVAGAAMPGEEVPTPRVGVADGPLAAALAARLSLVVPAGH
                     NAGFLAPFHVSTLGDPQLTDLLGRLGVRTLGDFAGLPEGAVLARFGPDVGRAHALAGG
                     RDDQVLRGEAPAEEMVASTEFDPPAERAETAAFVARALAADLVAQLEGRGLGCARVRI
                     DAETEHGESVSRLWRGDDLLGADAMVERLRWQLDGWLSGTTADPPPTAGITRLVLAAD
                     EVIPAGGRQLGLWGGASDTDRRAARGLDRLRGMLGPESVFTATLTGGRGPADRVVLVP
                     WGEPSPAPGSAALPWPGHHPWPAPALVHPSPIPVEVSGADGRGVTVSARGRLSATPAR
                     MSVAGDPWVAVIDWAGPWTSDERWWDLRARRHRARFQILTVGPRAHLCLLEDGRWWIE
                     ATYD"
     gene            1924..5076
                     /locus_tag="VF954_03095"
     CDS             1924..5076
                     /locus_tag="VF954_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX9530097.1"
                     /translation="MTEHAELHAHSNFSFLDGASHPEELVVEAARLGLSALAMCDHDG
                     LYGAVRAAEAARQVGLPTVFGAELTLGLAKRQNGIADPEGEHLVVLARDPDGYARLSR
                     AIAEAQLAGGAKGKPRFDLATLAATHRGHWLVLTGCRKGAVPAALAAAGPSAAGRALD
                     QLVDAFGRENVAVELWRHDDPVDSARNDALARLAISRGAELVATSNAHYATPAGYHLA
                     AALAAIRARRALDDMDGWLPAGPGAHLRSGAEQSRRFARWPGVVERAGELGRALAFDL
                     RLVAPNLPPFPVPAGHDEMSYLRHLVAEGATARYGSREAEAVPGAWAQIDHELAVIDQ
                     LGFPGYFLVVWDIVAFCRSRDILCQGRGSAANSAVCFALGVTNADAVKLGLLFERFLS
                     PERDGPPDIDVDIESGRREEAIQYVYERHGRHHAAQVANVITYRPRSAIRDAAKALGH
                     APGTVDAWSKQLERHGRLSVLVGERDPDGRPVHDIPDAVISLAAQMESFPRHLGIHSG
                     GMVICDRPIIEVCPVEWATMPGRTVLQWDKDDCAAVGLVKFDMLGLGMLEALHRTIDL
                     VAGHHGVTVDLAALPQEPEVYAMLSRADTVGVFQVESRAQMGTLPRLKPETFYDLVVE
                     VALIRPGPIQGGSVHPYLRRRNNEEEVTYLHPLLEPCLKKTLGVPLFQEQLMQMAIDV
                     AGFTPAEADQLRQAMGSKRSAERMEALRVRFYAGMAERGITGAVADEIFSKMAAFANF
                     GFPESHSVSFAYLVYSSAWLKLHYPAAFTAGLLSSQPMGFWSPRTLVADARRHGVEVR
                     RPDVNTSGAAAGLETVDGDRDHPVVRLGLASVKTLGDESAERVAANQPYRDLEDLARR
                     SGLTRAQLEALATAGATASLDGGDRRRALWAAGAVAQSGPGQLEGVVVGADAPPLPAM
                     TEVEEVVADLWATGITTGTSLTDLARAHLDERGVTLAATLPGVADGERVTVAGVVTHR
                     QRPETARGVTFVSLEDETGLVNVVCSIGVWARYRRVAQSAAAMVVRGRVEAAGGVVNV
                     VAERIEALELVAATRARNFS"
     gene            5130..5561
                     /locus_tag="VF954_03100"
     CDS             5130..5561
                     /locus_tag="VF954_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530098.1"
                     /translation="MRVTMMLCDAVQVADGKLYILGGGWSMTGPEPTPSAVAVKVDVD
                     LVEADQQHHWELWLEDADGRPVMVPTADGDRPVEVRGDFQVGRPVGVPEGSPVDLPLA
                     VNFGPIPLGPAGRYIWRLSIDGNTEENWRLAFSTRAAAPIA"
     gene            5558..6505
                     /gene="coaA"
                     /locus_tag="VF954_03105"
     CDS             5558..6505
                     /gene="coaA"
                     /locus_tag="VF954_03105"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEX9530099.1"
                     /translation="MSGETAWPSSYTDFDREEWSALRAATPLTLDEGDLAQLRGINES
                     ISLDEVSDVYLPLSRLLNLFVAASQDLHTVTDLFLGRLAGRVPYVIGLAGSVAVGKST
                     TARILQAVLSRWPDHPAVDLVTTDGFLYPNEVLEQRGLLDRKGFPESYDVRRLVKFLS
                     DLKSGRPEVSAPLYSHVSYDIVPGELQVVSRPDIVIVEGLNVLQAGKEPGREGRVVVS
                     DFFDFSIYVDADEADIEQWYVERFLTLCETVFQDAHSYFHQFAALDHDDAVATARRIW
                     RQINGTNLRENILPTRDRARLVLTKAPDHSVRRVRLRRM"
     gene            6594..7667
                     /locus_tag="VF954_03110"
     CDS             6594..7667
                     /locus_tag="VF954_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9530100.1"
                     /translation="MGVGELRWRLAADDGGQAALFPDDEIVHRRVGTGEFRGLEFLEV
                     NARRVINEVPAASRMPFRYTINPYRGCSHACAYCFARPTHEYLGLGIGEDFDRKLVVK
                     VNAVERVRAELRSPRWRGEHIAMGTNTDPYQRCEGKYHLTQGIVEALGEAANPFSILT
                     KSTLILRDIDRLAEAARRTEVRANFSIGTLDEDVWRLTEPGTPHPARRVAAVARLNEV
                     GIPCGVLVAPVLPGLSDHPDQLEAVVDAVVGAGAVSVSAVGLHLRPGVREHFMAWLEA
                     NRPELVELYRRRYRRGAYLPKTEQQELSDLVGELVGNARRRFGRPRAILAPDATGSGG
                     GRLPAPAPAARPDPAEQLGLGLM"
     gene            7918..>9035
                     /locus_tag="VF954_03115"
     CDS             7918..>9035
                     /locus_tag="VF954_03115"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9530101.1"
                     /translation="MHDSTAGYAGIDWATDAHAVCVVDDHGRVIVEFEVAHTADGLGD
                     LCRRIERAGARRAAIERPDGPVVDALLAAGLEVVVVASRSVKALRERYGTSGNKSDRS
                     DAYVLADALRTDGHRWRSLEPDSPATVTLRTHVRARKDLVETRVAVANQLRAHLRIVF
                     PGAVGVFRDLDSPISLRFLERFPSATRAAWLSEKRLGAWLRANGYCGRKSPADLHARL
                     VAAPAGIDGDEGDARGAITLAYVTVLKTLRAQIDELNTRLDELLDAHPDAAIFRSLPR
                     CATIRAATLLAEIGDCRARFPDAEALSCLAGVAPSTRASGRHRAVTFRFSSDKKLRDA
                     LCDFAGDSWRGNVWAEQRYRQLRASGKTHPHAERILAR"
BASE COUNT         1210 a         3285 c         3243 g         1297 t
ORIGIN      
        1 actcgctcga cgtggtggtg gtcagcccgc cgggtcggtg ccgaccggcg gacgctcgcc
       61 gcctggcggc tcgggcccgg gaccgtcacg gcgtcctcgt ggtggtcgag gaggttccgg
      121 tcgggcggac cggtcgcgtc ggacggtggc ccgagtcggt ggacctccag ctcgaagccg
      181 gtgcctccgt atggcacggg ctggatcggg gcgatggcac gctggcgtgt cgagaggtga
      241 cggtccgctc gttcggtcgg cgcagcggtg gccgggagcg ctcgacccgc ctgtggctac
      301 cggccgccga cggaggtccg ggtctcatcg gtggcgagcc gcgcacgggg tcgggcgcca
      361 acgggccagc cgccggcacg ttggcagggt aggtggccgt gcgcacgctg gcgctgtggt
      421 gcccggattg gccggtgacg gcggctgggt tcggcccgaa cgacccggtg gcggtgttgg
      481 acgccggtcg ggtcgtggcg gcggctgggg ccgcccgggg tgccggggtc agggccgggt
      541 tgcgtcggcg ggaggccgag agccgctgct ccgggctggt ggtcctcacc cgggatgtgg
      601 cggcggaggc ccgggccttc gaggcggtgg tcgctgcggt ggcgacgctg accccccgtg
      661 tggaggtgac ccgtccgggc ctgttggccc tcgacgcccg aggaccggcc cggtacttcg
      721 ggggtgagga gatgctgtgt cagcgggtgg cggtggtggc tggcgcggcg atgcccggcg
      781 aggaagtacc gacgcccagg gtgggggtgg ccgacgggcc ccttgccgcc gcgctggccg
      841 cccgactgag cctggtcgtg ccggccgggc acaacgccgg gtttctggcg ccgttccacg
      901 tctcgacgtt gggcgaccct cagctgaccg atctgctcgg ccggctcggt gtccgcacgc
      961 tcggcgactt cgccgggttg cccgaggggg cggtgctggc ccggttcggt cccgacgtcg
     1021 gccgagccca cgcgctcgcc ggcgggcgcg acgaccaggt gctccggggc gaggcgccag
     1081 ccgaggagat ggtggcgtca accgagttcg acccgccggc cgagcgggcg gagacggcgg
     1141 ccttcgtcgc ccgcgccttg gcggctgacc tggtggccca gttggagggt cgcgggctgg
     1201 gatgcgcacg ggtgcggatc gacgccgaga ccgagcacgg ggagtccgta tcacggctgt
     1261 ggcggggcga cgacctgctc ggtgccgacg ccatggtcga acggctgcgt tggcagctcg
     1321 acgggtggct ctcgggcacg accgccgatc ctcccccgac tgctggcatc acccgcctgg
     1381 tgctcgcggc cgacgaggtc atccctgccg gaggtcgtca gctcggcctg tggggcggcg
     1441 cctcggacac ggatcgtcgt gccgctcggg gtctcgacag attgcgaggg atgctcggtc
     1501 ccgagtccgt gttcaccgcc accctcaccg gaggccgtgg cccggccgac cgcgtggtgc
     1561 tcgtcccctg gggagagccg tcaccggccc cggggtcggc ggccctgccg tggcccggcc
     1621 atcacccatg gcccgcaccg gcgctcgtcc atccctcgcc tataccggtc gaggtctccg
     1681 gcgccgacgg tagaggggtg acggtgagcg ctcggggacg tctcagcgcc accccggcac
     1741 ggatgtcggt cgccggtgac ccgtgggtgg ccgtcatcga ctgggccggg ccgtggacct
     1801 cggacgagcg gtggtgggac ctccgggcca ggcgccaccg ggcccggttc cagatcctca
     1861 ccgtcggccc gcgcgcccat ctgtgcctgc tcgaggacgg gcgctggtgg atcgaggcca
     1921 cctatgactg agcacgcgga gctgcacgcc cattcgaact tcagcttcct cgacggcgcc
     1981 tcgcaccccg aggagctggt cgtcgaagcg gcgcggctgg gtctgtccgc gctggcaatg
     2041 tgcgatcacg acggtctcta cggtgcggtc cgggccgccg aggcggcccg ccaagtcggg
     2101 ctgccgacgg tgttcggagc cgagctgacc ctcgggctgg ccaaacgcca gaacggcatt
     2161 gccgaccccg aaggcgagca cctggtggtc ctcgcccgcg accccgatgg ctacgcccgg
     2221 ctctctcggg ccatcgccga ggcccagctc gccgggggcg ccaagggcaa gccgcgcttc
     2281 gacctcgcca ccctggcggc gacccaccgc ggtcattggc tcgtcctcac cggttgccgg
     2341 aagggagcgg tcccggccgc gctggccgcg gcaggaccct cggcggcggg acgcgccctc
     2401 gaccagctcg tcgacgcctt tgggcgggag aacgtggccg tcgagctgtg gcgccacgac
     2461 gacccggtcg actcggcccg caacgacgcc ctggcccgcc tggccatctc gcggggcgcc
     2521 gagctggtgg caaccagcaa cgcccactac gccaccccgg ccggctacca cctggcggct
     2581 gcgctggcgg ccatacgcgc ccggcgggcg ctcgacgaca tggacggttg gctcccggcc
     2641 ggccccggtg cccatctgcg ctcgggggcc gagcagtccc ggcggttcgc ccgttggccg
     2701 ggggtggtgg agcgggccgg cgagctcggc cgggccttgg ccttcgacct gcgactggtg
     2761 gcgccgaacc tgcccccgtt ccccgtgccg gcgggacacg acgagatgag ctacctgagg
     2821 cacctggtcg ccgagggcgc cacggcccgc tacggctcgc gggaggccga ggcggtgccg
     2881 ggcgcctggg cccagatcga ccacgagctg gccgtcatcg accagctcgg ctttcccggc
     2941 tacttcctcg tggtgtggga catcgtggcc ttctgccgca gccgcgacat cttgtgccag
     3001 gggcgcggct cggccgccaa cagcgccgtg tgcttcgccc tgggggtgac gaacgccgac
     3061 gccgtgaagc tcggcctgct cttcgagcgg ttcttgagcc ccgaacgtga tggcccgccc
     3121 gacatcgatg tggacatcga gtccggccgg cgggaggagg cgatccagta cgtatacgag
     3181 agacacggcc gccaccacgc cgcccaggtc gccaacgtca tcacctaccg accgcgctcg
     3241 gccatcaggg acgcggccaa ggccctcggc catgcgcctg gcaccgtcga cgcctggtcg
     3301 aagcaactgg aacggcacgg ccggctgagc gtcctggtcg gcgagcgcga ccccgacggc
     3361 cgcccggtcc acgacatccc cgacgccgtg atctccctgg ccgcccagat ggagagcttc
     3421 ccccgccacc tcggcatcca ctccggcggc atggtgatct gcgaccggcc gatcatcgag
     3481 gtctgcccgg tcgagtgggc gaccatgccg gggcgcacgg tgctccagtg ggacaaggac
     3541 gactgcgccg cggtcggcct ggtcaagttc gacatgctcg gcctgggcat gttggaggcg
     3601 ctgcaccgga ccatcgacct ggtggccggc caccacgggg tcaccgtcga cctggccgcc
     3661 ctgccccagg agcccgaggt ctacgccatg ctcagccggg ccgacacggt cggggtgttc
     3721 caggtcgaga gccgcgccca gatgggcacc ctgccccggc tcaagcccga gaccttctac
     3781 gacctcgtcg tggaggtggc gctcatccgg cccggcccga tccagggcgg ctcggtgcat
     3841 ccgtacctgc ggcgccggaa caacgaggag gaggtcacct acctccaccc gctcctcgag
     3901 ccgtgcctga agaagaccct cggggtgccg ctgttccaag agcagctcat gcagatggcc
     3961 atcgacgtgg ccggcttcac cccagccgag gccgaccagc tccgccaggc catgggctcg
     4021 aaacgctcgg ccgagcgcat ggaggcactg cgggtccgct tctacgccgg gatggccgag
     4081 cggggcatca ccggggcggt ggccgacgag atcttctcca agatggcggc gttcgccaac
     4141 ttcgggttcc ccgagtcgca ctcggtgagc ttcgcctacc tcgtctactc ctcggcctgg
     4201 ctgaagctgc actacccggc ggccttcacc gctggcctgt tgagctccca gccgatgggg
     4261 ttctggtcgc cccggaccct ggtggccgac gcccgccgcc acggggtcga ggtgcgccgg
     4321 cccgacgtga acacgagtgg tgccgccgcc ggcctggaaa ccgtcgacgg tgaccgcgac
     4381 catcccgtcg tccggctcgg gctggcgtcg gtcaagaccc tcggggacga gtcggccgag
     4441 cgggtcgcgg cgaaccagcc ctaccgcgac ctcgaagacc tggcccgccg ctccggtctc
     4501 actcgggccc agttggaagc gctcgccacc gccggggcga cggccagcct cgacggcggg
     4561 gaccgccgcc gagcgctgtg ggctgcgggc gcggtcgccc aatccggtcc cggacagctc
     4621 gaaggggtcg tcgtcggggc cgacgcgccg ccgctgccgg cgatgaccga ggtcgaggag
     4681 gtggtcgccg atctgtgggc cacgggtatc acgaccggga cgtcgctcac cgatctggcc
     4741 cgggcgcatc tcgacgagcg gggggtgacc ctggcggcga cgctaccggg cgtcgccgac
     4801 ggggagaggg tgaccgtggc cggggtggtg acgcatcgcc agcggcccga gacggcccgg
     4861 ggcgtcacct tcgtctccct ggaggacgag accgggctcg taaacgtggt ctgctcgatc
     4921 ggggtgtggg ctcgctaccg gcgcgtcgcc cagtccgcgg cggcgatggt ggtgcgaggc
     4981 cgggtcgagg cggcgggcgg ggtggtcaat gtggtggccg aacggatcga ggcgctggag
     5041 ctggtcgccg ccacgagggc gcgcaacttc tcctgaaccg ctccggtgcc gtgcccggcg
     5101 cccaccgcgg acgcactagc ttgctgcggg tgcgggtcac gatgatgctc tgcgacgcgg
     5161 tccaggtggc ggacggaaag ctctacatcc tcggcggagg gtggagcatg accggtcctg
     5221 agcccacacc gtccgccgtc gccgtcaagg tcgacgtcga cctggtcgag gcggaccagc
     5281 agcaccactg ggagctctgg ctcgaggacg ccgacgggcg tcccgtcatg gtgcccaccg
     5341 ccgacggcga ccgcccggtc gaggtgcggg gcgacttcca ggtgggccgg ccggtcggcg
     5401 tccctgaggg cagccccgtc gaccttcctc tcgcggtcaa cttcggtccc atcccgctgg
     5461 gcccggccgg ccgctacatc tggcggctga gcatcgacgg caacaccgag gagaactggc
     5521 gcctcgcctt cagcactcga gccgccgcgc ccatcgcgtg agcggcgaga ccgcctggcc
     5581 gtcgagctac accgacttcg accgggagga gtggagcgcc cttcgcgccg ccacccctct
     5641 cacgctcgac gagggtgatc tggcccagct gcgcggcatc aacgagagca tctcgctgga
     5701 cgaggtcagc gacgtgtacc tcccgctgtc ccggctcctc aacctcttcg tggccgccag
     5761 ccaggacctg cacacggtca ccgacctgtt cctcggccgg ctggcgggcc gggtccccta
     5821 cgtcatcggc ctcgccggct ccgtcgcggt gggcaagagc accacggcgc gcatcctcca
     5881 ggcggtgctg tcccgctggc ccgatcaccc ggccgtggac ctggtgacca cggacggctt
     5941 cctgtatccc aacgaggtcc tcgagcagcg tgggctgttg gaccgtaagg gcttcccgga
     6001 gagctacgac gtgaggcgcc tcgtgaagtt cctgtccgac ctgaagtcag gccggcccga
     6061 ggtcagcgcc ccgctctact cccacgtctc ctacgacatc gtgcccggcg agctccaggt
     6121 cgtcagccgg cccgacatcg tgatcgtcga gggcctcaat gtgctccagg ccggcaagga
     6181 gccagggcgg gagggccggg tggtggtgtc ggacttcttc gacttctcca tctacgtcga
     6241 cgccgacgag gcggacatcg agcagtggta cgtcgagagg ttcctcacct tgtgcgagac
     6301 cgtcttccag gacgcgcact cgtacttcca ccagttcgcc gccctcgacc acgacgacgc
     6361 cgtcgccacc gcccggcgga tctggcgcca gatcaacggc accaacctca gggagaacat
     6421 cctgcccacc cgggaccggg cccggctggt gctgaccaag gcgccggacc acagcgtgcg
     6481 ccgggtgcgt ctccgccgga tgtgacctcc aggtcacgcc cgtcccgggc cgcggcacac
     6541 gacacggcag cgcgccctgg acggggggaa cgcatgttcg ttacaatgcc gcgatgggcg
     6601 tgggcgagtt gcgctggcgg ctggcggccg atgacggggg ccaggccgcc ctgtttcccg
     6661 acgacgagat cgtgcaccgt cgggtgggca cgggcgagtt ccggggcctc gagttcctcg
     6721 aggtcaatgc ccggcgggtg atcaacgagg tgccggccgc ctcccgcatg ccgttccggt
     6781 acaccatcaa cccctaccgg ggatgcagtc atgcgtgtgc ctactgcttc gcccggccga
     6841 cgcatgagta cctcgggctc ggcatcggcg aggacttcga ccgcaagctc gtggtcaagg
     6901 tcaacgcggt cgagcgggtg agggctgagc tgcggtcccc gaggtggagg ggcgagcaca
     6961 tcgccatggg caccaacacc gatccctacc agcgctgcga aggcaagtac cacctgaccc
     7021 aggggatcgt cgaggccttg ggcgaagcgg ccaacccgtt ctcgatcctg accaagtcaa
     7081 cgctgatcct gcgcgacatc gaccgtctgg cggaggcggc gcgccgcacc gaggtgcgcg
     7141 ccaacttctc catcggcacg ctcgacgagg acgtctggag actcacggag ccgggcacgc
     7201 cgcacccggc acgaagggtc gcagccgtgg ccaggctcaa cgaggtgggc atcccgtgcg
     7261 gtgtgctcgt ggcgccggtc ctgcccgggc tctccgacca ccccgaccag ctcgaggcgg
     7321 tcgtcgacgc cgtggtcggg gcgggtgcgg tgagcgtctc ggccgtcggg ttgcacctcc
     7381 ggccgggcgt ccgtgagcac ttcatggcat ggctggaggc caaccgcccc gagctcgtcg
     7441 agctctaccg ccgccgctac cggaggggcg cgtacctgcc caagaccgag cagcaggagc
     7501 tctccgacct ggtgggcgag ctggtcggga acgcgagacg gcgcttcggc cgcccccgcg
     7561 ccatcctggc tcccgacgcg accggctcgg gtggagggcg tctcccggct cccgctcccg
     7621 cggcccgtcc agaccccgcc gagcagctcg ggctcggatt gatgtagacg ctcactggtc
     7681 caccatcccc gtgtcggtgg ccgtcaggct gtcgatgcac ccctggtgtc caccgggcca
     7741 tcgccacgtt cgcttgcggc tcgaccgcca cgatgagtcc cgcgaggtcc ggtggcccag
     7801 gacgtgccga ctggcaatag ggctgagcgc cccagagcgc cgatcagtga gcgaccggca
     7861 gacacgtcct gagaccaccg ggtgcttccc cactgcagca acagcgagga ggctgacatg
     7921 catgattcca ccgccggcta cgccggcatc gactgggcca ccgacgcgca cgccgtctgc
     7981 gtggtcgacg accacggccg ggtgatcgtc gagttcgagg tggcccatac cgccgacggg
     8041 cttggcgacc tgtgccgtcg catcgaacga gccggcgccc gcagggcggc tatcgagcgc
     8101 cccgacggac ctgtcgtcga cgcgctgctc gccgccggcc tggaggtcgt ggttgtggcc
     8161 agccggtcgg tgaaggcgct ccgggagcgc tacgggacgt cgggaaacaa gtcggaccgc
     8221 tccgacgcct acgtcctggc cgacgcgctg cgcaccgacg gccaccgctg gcgatccctc
     8281 gagcccgaca gcccagccac ggtcaccctg cgtacccacg ttcgagcccg caaggacctg
     8341 gtcgagactc gagtcgccgt cgccaaccag ctccgcgccc acctgcgcat cgtgttcccc
     8401 ggtgcggtcg gggtgttccg cgacctcgac agcccgatct cgctgcgatt cctcgagcgg
     8461 ttcccgtccg ccacccgcgc cgcgtggctg tcggagaaac gcctcggcgc ttggctgcgc
     8521 gccaacggct actgcggacg caagagccca gccgacctgc acgcccggct cgtcgctgca
     8581 cccgccggca tcgacggcga cgaaggcgac gctcgcggcg ccatcacgct cgcctacgtc
     8641 accgtcctca aaaccctgag agctcagatc gacgaactga acacccgtct cgacgagctc
     8701 ctcgacgcgc accccgacgc cgccatattc cggtccctgc cgcgctgcgc caccatccga
     8761 gccgccacgc tgctcgccga gatcggcgac tgccgcgcaa ggttccccga cgccgaagcc
     8821 ctgtcgtgtc tcgccggtgt cgctccctcc acccgcgcca gcgggcggca ccgtgccgtc
     8881 accttccgct tcagcagcga caagaagctc cgcgacgccc tctgcgactt cgccggagac
     8941 agctggcgcg gcaacgtctg ggccgaacag cgctaccgac agctgcgtgc gagcggcaaa
     9001 acccaccccc acgccgaacg gatcctcgcc cgcag
//
LOCUS       DASWGG010000170         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1141884, whole genome shotgun sequence.
ACCESSION   DASWGG010000170 DASWGG010000000
VERSION     DASWGG010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141884"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VF954_03120"
     CDS             complement(<1..484)
                     /locus_tag="VF954_03120"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9530102.1"
                     /translation="MTTTCFHHRDQETGRSCTRCGRPACSACLHEAPVGAHCWECIKA
                     ARPPASQRVRRWNATAGPVVTIGLVAVNVIVYVLTLGQPGSSTAASLTDRLSLYGPAV
                     AAGQWYRLVSSGFVHYSLIHIAFNMLLLYQLGIMLESELGRWRFAGLYFAALLCGSFG
                     V"
     gene            complement(548..1426)
                     /locus_tag="VF954_03125"
     CDS             complement(548..1426)
                     /locus_tag="VF954_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9530103.1"
                     /translation="MPTVSVVTGANSGIGRAIAIHLAAHGHEVYGTVRDPNRAEKLRS
                     MSADAGVEVQLVTLDVADDDSVRRGMGEILDRAGQVDVLVNNAGVGGNAVVEEATPAG
                     IVDVVNVNVGGGVRCLQAVLPGMRARHRGAIVNITSVAGRFGAIAQAPYVASKWAFEG
                     VSEELAFELAPFGIRVAIVEPGVTRSAIFAKNIDAPNQTGAYGDHYRRLFEFYAAGVA
                     HATDPFEVAAVVYEAITTSTPRLRYPCSWGGPEIISGRRMLTDEDWVAMGAIGDDEEY
                     RAEFLRRFGVDITPPA"
     gene            1538..1831
                     /locus_tag="VF954_03130"
     CDS             1538..1831
                     /locus_tag="VF954_03130"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX9530104.1"
                     /translation="MILEHAALEVVPGREEAFETAFGRARGIISSAPGFRSLRLGRCI
                     EIPNRYVLLVEWERLEDHTEGFRGSPAFQEWRALLHHFYNPFPTVEHFQDVTT"
     gene            1911..>2612
                     /locus_tag="VF954_03135"
     CDS             1911..>2612
                     /locus_tag="VF954_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9530105.1"
                     /translation="MESFTGKRAVVTGGGSGMGRELVVQLASAGCSVATCDVSPAGLA
                     ETAAKAEAGAPAGTVITTHQCDVSDEAQVLAFRDEVVARHGDKLNLVFNNAGISGGGS
                     FLTDTRQSWEQTFAVDWWGVYHCCRAFLPLLVAGDDGYLVNTSSVNGFWATLGPGMPQ
                     TAYSAAKFAVKGFSEGLIEDLRAHAPHVKVAVVMPGHIGTSIFMNSRRYLGNPDPDEM
                     TEADLEPLRANLARRG"
BASE COUNT          411 a          901 c          928 g          372 t
ORIGIN      
        1 gcaccccgaa cgagccgcac agcagggcgg cgaagtacag cccggcgaag cgccaccggc
       61 cgagctccga ctcgagcatg atgcccagct ggtacagcag cagcatgttg aaggcgatgt
      121 ggatgaggct gtagtgcacg aacccggagc tgaccagccg gtaccactgc ccggcggcga
      181 ccgccggccc gtacagcgac agccggtcgg tcagggaggc cgcggtcgag ctcccgggct
      241 ggcccagggt cagcacgtac acgatgacgt tgacggcgac gagaccgatg gtgacgacgg
      301 gaccggccgt ggcgttccag cggcggacgc gctgcgaggc gggcgggcgg gccgctttga
      361 tgcactccca gcagtgggcg ccgaccggtg cctcgtgcag gcaggccgag caggccggcc
      421 ggccgcagcg ggtgcacgac cgcccggtct cctggtcccg gtggtggaag cacgtggtgg
      481 tcatcgccca ggatcgtacg ggaacggtgc gaggcggctc gcgtcaggcg gtcgcctgcc
      541 acctcggtca ggccggcggg gtgatgtcca caccgaaccg tcgcaggaac tctgcccggt
      601 actcctcgtc gtcgccgatc gcgcccatgg ctacccagtc ctcgtcggtc agcatccgcc
      661 gcccggagat gatctccggc ccgccccacg agcacggata gcgaagccga ggagtgctgg
      721 tggtgatggc ctcatacacg acggcggcca cctcgaacgg gtcggtggca tgggcgacgc
      781 ccgcggcata gaactcgaag aggcgccgat agtggtcgcc ataggcgccg gtctggttcg
      841 gggcgtcgat attcttggca aagatcgccg atctcgtcac gcccggctcg acgatggcga
      901 cccggatgcc gaacggggcc agctcgaacg ccagctcttc gctcactccc tcgaacgccc
      961 acttcgacgc gacgtagggc gcctgggcga tggcgccgaa ccgtccggcc accgacgtga
     1021 tgttgacgat cgcgcccctg tgtcgggcgc gcatcccggg cagcaccgcc tggaggcaac
     1081 gcacgcctcc cccgacgttg acgttcacca catccacgat gccggccggc gtggcctcct
     1141 ctacgacggc gttaccaccc accccggcgt tgttgaccag cacgtccacc tggccggcac
     1201 gatcgaggat ctcgcccatg ccccggcgga ccgagtcgtc gtcggcgacg tcgagcgtca
     1261 cgagctggac ttcgactccg gcgtcggcgg acatggaccg cagcttctcc gcacggttcg
     1321 ggtcccgtac ggtgccgtag acctcgtggc cgtgggccgc gaggtggatg gcgatcgcac
     1381 ggccgatccc cgagttggcg cccgtgacga cactcaccgt cggcatcggc ggactgtatc
     1441 ccggggatcc ccgctcccga cggtcgagcc cgccagctcg cgttcgagca ccgatgcgcc
     1501 ggcggcggcg cctgcccggc gtatggttgg gctccccatg atcctcgaac acgccgcgct
     1561 cgaagtggtc ccgggccggg aggaggcctt cgagaccgcc ttcggccggg ccagaggcat
     1621 catctcgtcc gcgccgggct tccgcagcct ccggttgggc cgatgcatcg agatccccaa
     1681 ccgctacgtg ctgctggtcg agtgggagag gctcgaggac cacaccgagg gcttccgggg
     1741 ctcgcccgcg ttccaggagt ggcgggcgtt gctccaccac ttctacaacc cgttcccgac
     1801 ggtcgagcac ttccaggacg tgaccaccta gcgcgcttga ccggcgcggc cctagggttt
     1861 gccggccaac aaagccaaca gagccaagag ccaatagaga ggagccgggc gtggagtcct
     1921 tcacgggcaa gcgggcggtg gtgaccggcg gcggatccgg aatggggcgg gagctggtgg
     1981 tccagctggc gtcggcggga tgctcggtgg cgacgtgcga cgtgagcccg gccgggctgg
     2041 cggagacggc cgccaaggcg gaggccgggg cgccggccgg caccgtgatc accacccatc
     2101 agtgcgacgt ctcggacgaa gctcaggtgc tggcgttccg ggacgaggtg gtggcgaggc
     2161 acggggacaa gctcaatctg gtcttcaaca acgccgggat cagcggcggc ggaagcttcc
     2221 tgaccgacac gcgccagtcg tgggagcaga cgttcgcggt ggactggtgg ggcgtctacc
     2281 actgctgccg agcgttcctg ccgcttctgg tcgccggcga cgacggctac ctggtcaaca
     2341 ccagcagcgt caacggcttt tgggccacgc tcggcccggg catgccccag accgcgtaca
     2401 gcgcggcgaa gttcgccgtc aagggcttct ccgagggcct gatcgaggac ctgcgagccc
     2461 acgcgccgca cgtgaaggtc gcggtggtga tgcccggcca catcgggacg agcatcttca
     2521 tgaactcgcg ccgctacctg ggcaacccgg accccgacga gatgaccgag gccgatctgg
     2581 agccgctgcg tgccaatctg gcccgccggg gc
//
LOCUS       DASWGG010000171         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1143150, whole genome shotgun sequence.
ACCESSION   DASWGG010000171 DASWGG010000000
VERSION     DASWGG010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143150"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1790
                     /locus_tag="VF954_03140"
     CDS             <1..>1790
                     /locus_tag="VF954_03140"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HEX9530106.1"
                     /translation="TPAPTSRTHISLASQTPWVAPGGIFDARLTVDTDQPLPSLELIV
                     TVYFPLRTRSDFARSLDGHVESPLRSSPSTTLDQLQRDQAGAVSVVMPVQDPAKPTDP
                     TALPLRREGVYPVQVEVRGAGGGPLLDRLTTHLLFFPPIDPTSGQARMDFAWALPLTA
                     PVALQTGGSSQLDPAARATLSDLSQALTSPDHLAEPVDLVPTPETMHALSASADPTDR
                     ATLASLARAAQATEVVDGTYVPVDLDAMVGQGLGDELTAQRDRAERVLQSSLGLAPAP
                     GLRVVNGRLGDAALSRLRDEDVSKLVLPEEAFTPESRVTTLASPFVVDDHRLTHAEAA
                     SGDPGLAAHFAKGDQVLLAHQLLADLAQIYEDSPGAPRAVVALPPPGWVPTASFVNAV
                     LDGMTASPVIAPTTLSTIFNGTVPLSPGKRRGTADLRTLVAPATPAGLPAEAIRAARR
                     RLKAFSSLLDEDATLPDALDQQLLAAEAATLRAHDRQAMVSAVEAFLDKQRGLVHLPS
                     ARSVTLTSRGGNIPLTILSDADYPIRAVIRFQSDKLAFPGGTAVTVDLSPRRNTTEVF
                     AVRARASGSFPVTVRLTSSDGQFDLGQSQLT"
BASE COUNT          245 a          741 c          585 g          219 t
ORIGIN      
        1 accccggcgc cgaccagcag gacccacatc agcctggcgt cccagacgcc gtgggtggca
       61 ccgggcggga tcttcgacgc ccgcctcacc gtcgacaccg accagcccct ccccagcctc
      121 gagctgatcg tcacggtgta cttcccgctg cgcacccggt ccgacttcgc ccgcagcctg
      181 gacgggcacg tggagagccc gctgcgcagc tcgccgagca cgacgctgga ccagctccag
      241 cgcgaccagg ccggcgccgt cagcgtggtc atgccggtgc aggacccggc caagcccacc
      301 gacccgaccg cgctgccgct gcgccgggag ggcgtctacc ccgtccaggt cgaggtgagg
      361 ggcgccgggg gcgggccgct gctcgaccgg ctgaccaccc acctgctgtt cttccccccg
      421 atcgacccca cctcgggcca ggcccgcatg gacttcgcct gggccctgcc cctgaccgcc
      481 ccggtcgccc tccagaccgg cggcagctca cagctggacc ccgcggcccg ggccacgctc
      541 agcgacctgt cccaggcgct cacctcgccc gaccacctgg ccgagcccgt cgacctggtg
      601 cccacccccg agaccatgca cgccctgtcg gccagcgccg accccaccga ccgggccacg
      661 ctcgcctccc tggcgagggc ggcgcaggcc accgaggtgg tggacggtac ctacgtgccc
      721 gtcgacctcg acgccatggt cggccagggc ctcggcgacg agctgaccgc gcagcgcgac
      781 cgggccgagc gagtgctcca gtcgagcctc gggctggcgc ccgcgccggg actgcgggtg
      841 gtgaacggcc gcctcggcga cgccgccctg tcgcgcctgc gcgacgagga cgtgagcaag
      901 ctcgtgctgc cggaggaagc gttcacgccg gagagccggg tgacgaccct ggcctcgccc
      961 ttcgtggtcg acgaccaccg gctcacccat gccgaggccg catccggcga tcccgggctg
     1021 gccgcccatt tcgccaaggg cgaccaggtc ctgctagccc accagctcct cgccgacctg
     1081 gcccagatct acgaggacag tcccggtgct ccccgggcgg tggtcgcact gccgccgccg
     1141 gggtgggtcc cgacggcgag cttcgtcaac gcggtcctcg acgggatgac ggccagcccg
     1201 gtgatcgccc cgaccaccct gtcgaccatc ttcaacggga cggtgccgct gtcgccgggc
     1261 aagagacgcg gcacagcgga cctccgcacc ctcgtcgccc cggccacgcc cgctggcctc
     1321 ccggccgagg ccatccgggc cgcccgccgc cggctgaagg ccttcagcag cctgctcgac
     1381 gaggacgcca ccctgcccga cgccctcgat cagcagctcc tcgcggccga ggccgccacc
     1441 ctccgggcgc acgaccgcca agcgatggtc agcgccgtcg aggccttcct ggacaaacag
     1501 cgcggcctgg tccacctgcc cagcgcccgg tccgtcacgc tgacctcccg cggcgggaac
     1561 atcccgttga ccatcctctc cgacgccgac taccccatcc gagctgtcat ccggttccag
     1621 agcgacaagc tggccttccc gggcggcacc gcggtgaccg tggacctcag ccccaggcgg
     1681 aacacgaccg aggtgttcgc ggtccgcgcc agggcatcgg ggtcgttccc ggtcacggtg
     1741 cggctgacgt cgtcggacgg gcagttcgac ctggggcaga gccagctcac
//
LOCUS       DASWGG010000172         5808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1143289, whole genome shotgun sequence.
ACCESSION   DASWGG010000172 DASWGG010000000
VERSION     DASWGG010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143289"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1603)
                     /locus_tag="VF954_03145"
     CDS             complement(95..1603)
                     /locus_tag="VF954_03145"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX9530107.1"
                     /translation="MASNDETEEGQRQPPRPPAEGVRIIGAEEAAAALDSGQAAGRRP
                     EDAPRYGDVPPAPAGPRPVHRFPLPDSVDPATAVPRTPPERAPIEGAAERPAGDRRPQ
                     PGAEPGSVNVGTGKAPDLPHWTEPATGEVPQILAGEQGETDDLGAWSGLSRGGPRWRG
                     EGHADWQEADYGDAFGGGDLVGVPSREGVADPYGFEEAPAAPDKAALAPAPRPIRTRP
                     RSGHVRPSANGRDVGTAVITGGAIGIGAIILFKLGSATAMFLVTLVVTACAVELFAAF
                     RRAGHRPATLLGLVATFALLVASYEKGETAIPLILAMTVVFSMLWYLFGVVRGRPAVN
                     VAMTVLGVAWVGLLGSFGALLLDPRLVANHNRRGVSELFAAILCTVAFDVAAFFVGSQ
                     IGRRAFAAGVSPNKTWEGTAAGIVAAVFMGAVVGGLHFHPWTFSRGLALGVVVGMVAP
                     LGDLCESMIKRDLGLKDMGQILPGHGGLLDRFDAMLFVLPATYYLVRLLKMG"
     gene            complement(1656..2225)
                     /gene="frr"
                     /locus_tag="VF954_03150"
     CDS             complement(1656..2225)
                     /gene="frr"
                     /locus_tag="VF954_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX9530108.1"
                     /translation="MDDSLIDAALEEARDHMAKAVAHTQSEFASVRTGRASPALVEKL
                     KVDYYGSEVPLQQLAGFNVPEARLLVVTPYDKSAIKAVEKAIQNSDLGINPSNDGLVI
                     RLAFPPLTEERRKEMVKVVRHKAEEGRVAVRNSRRAVRHELEALEKDGDISSDELERA
                     EKELERITHEFVGEIDRVLSHKEQELLEV"
     gene            complement(2225..2971)
                     /gene="pyrH"
                     /locus_tag="VF954_03155"
     CDS             complement(2225..2971)
                     /gene="pyrH"
                     /locus_tag="VF954_03155"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX9530109.1"
                     /translation="MGERGPDGGKPRWRRVVLKMSGEALASAASDETIDGETVERIAV
                     ELAEARRELDVEIAVVMGGGNIWRGATGAVAGMDRATSDYMGMLGTVINALALQDALE
                     RHGQPTRVLSAIQMAQIAEPYIRRRAIRHLEKGRVVIFAAGMGSPFFTTDTPAALRAV
                     EIDAEAILKGTHSGVDGVYSADPRLDPTALRFDEVTFGEVIAQDLRVMDMTAITFCMD
                     NTLPILVFDLMEPGNIRRALVGDPIGTLIR"
     gene            complement(2975..3799)
                     /gene="tsf"
                     /locus_tag="VF954_03160"
     CDS             complement(2975..3799)
                     /gene="tsf"
                     /locus_tag="VF954_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX9530110.1"
                     /translation="MRDMSEFTAKDVQALRQATGAGMMDAKRALEANAGDAEAAAKWL
                     REKGLGKAADRTDRENAQGAVAVSAAGRVAALVELKSETDFVAKSPDFTKLAQEIADL
                     VVEKGEGAAAERADQIDDLKVTLKENISLGRVVRFEAGPDRVLDTYLHVQSGRGVNAV
                     LVEVAGAPAEAANDLAHDIAVHIAFARPQHVAREEVPAADVAAERASLEVQTRNEGKP
                     EAALAKIVEGKLNGWYKRFPGGVLLEQPYAKDDKRSVEQVLGGAQVVRFAQVVIGG"
     gene            complement(3834..4742)
                     /gene="rpsB"
                     /locus_tag="VF954_03165"
     CDS             complement(3834..4742)
                     /gene="rpsB"
                     /locus_tag="VF954_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX9530111.1"
                     /translation="MAVVTMKQLLEAGVHFGHQTRRWNPKMRRFIFGERNGIYIIDLN
                     QTLRRIEVAYTFTRDLVAGGGSVLFVGTKKQTQDPIADYAQRCGMPFVNERWLGGMLT
                     NFATISGRVKKMQEYERMRAAGDFDAMPKKEALILSRELDKLQRNLGGLRSMARLPEA
                     LFVIDTKKEHIAVTEANKLGLPIVAVVDTNCDPDVIDYVIPGNDDAIRSGTLMCRIIA
                     DAVDEGRFIASRKASRAGGGPGAAASAGPGPIDPEEEARRAQHQAEARRQAALQAQER
                     EARLAAAPPADGASPAPAEATDSAEA"
     gene            complement(4900..>5808)
                     /locus_tag="VF954_03170"
     CDS             complement(4900..>5808)
                     /locus_tag="VF954_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530112.1"
                     /translation="DAFRDGLNRQETPPAALPVTVPSDRFTVGTDGAAQTAVIDGGGW
                     GHGIGMSQYGALGKALRGMKAADILAAYYGGLRPVTLQPEQLPARLRVALSTGLASAA
                     VTAPGSFRVLDGAGRPLAVAATGVWTVSPGPAPGQVRVVPPPDQAGPPVVTAARITPS
                     HPDAGEPVTVAFRVAPGARVALTRRDPDGTTAGRDDGVAAGAVTEALVAGTLPGSYGV
                     TVTADAGGGRVTTVPLAFTVPAPAGEPAGRALGRRAALPSGPGTRVTGWPGLLAALGL
                     ASVVVAGLVVGRRRPAADAPGQAQPS"
BASE COUNT          731 a         2119 c         2122 g          836 t
ORIGIN      
        1 aagcgctccg gggcggaggc gatcacctcc agggcctggg tgccgatgga tccggtcgag
       61 cccaccacac tgacgctcgt catcccgctg ggcgtcatcc catcttgagc agccgcacga
      121 ggtagtacgt ggccggcagc acgaacagca tggcgtcgaa gcggtccagc agcccgccgt
      181 ggccgggcag gatctggccc atgtccttga gcccgagatc ccgcttgatc atggactcgc
      241 acaggtcgcc cagtggcgcc accatgccga ccaccacgcc gagggccagg ccccgggaga
      301 aggtccacgg gtggaagtgg aggccgccca cgacggcccc catgaagacg gccgccacga
      361 tgccggcggc cgtgccctcc cacgtcttgt tggggctcac gccggccgcg aacgcccggc
      421 gcccgatctg gctgcccacg aagaaggcgg ccacgtcgaa cgccaccgtg cacaggatgg
      481 cggcgaacag ctcgctgacg ccgcgccggt tgtggttggc gaccaggcgg gggtccagca
      541 gcagggcgcc gaaggagccc aacagcccga cccacgccac gccgaggacg gtcatggcca
      601 cgttgaccgc gggccggccc cgcaccacgc cgaacaggta ccacagcatc gagaacacga
      661 cggtcatggc caggatcagc gggatcgccg tctccccctt ctcgtagctg gcgaccagca
      721 gggcgaaggt ggcgaccagg ccgagcaggg tggcgggccg gtgaccggcc cggcggaagg
      781 cggcgaacag ctccaccgcg cacgccgtga cgaccagggt gacgaggaac atcgccgtgg
      841 ccgagcccag cttgaacaag atgatggcgc cgatgcctat ggccccgccc gtgatgaccg
      901 cggtgcccac gtcccggccg ttggccgagg gccgcacgtg gccggaccgg ggccgggtcc
      961 ggatgggccg cggggccggg gcgagggccg ccttgtcggg ggctgccggg gcctcctcga
     1021 acccgtaggg gtcggccacc ccctcccgcg acggaactcc caccaggtcg ccgccgccga
     1081 acgcgtcgcc gtagtcggcc tcctgccagt cggcgtggcc ctcgccccgc cagcggggac
     1141 cgccccgcga caggcccgac caggccccga ggtcgtcggt ctcgccctgc tcgccagcca
     1201 ggatctgggg cacctcgccc gtggccggct cggtccagtg cgggaggtcc ggggccttgc
     1261 ccgtcccgac gttgacgctg ccgggctcag cgccgggctg gggccggcgg tccccggcgg
     1321 ggcgctcggc ggctccctcg atgggggctc gctcgggcgg ggtgcggggc accgccgtgg
     1381 ccgggtcgac cgagtcgggc agcgggaagc ggtgcaccgg ccggggcccc gcgggcgcgg
     1441 gcggcacgtc gccgtagcgc ggggcgtcct cggggcgccg gcctgcggcc tggcccgagt
     1501 ccagggcggc ggcggcctcc tcggcgccga tgatccgcac cccttcagcc gggggacgag
     1561 ggggttgccg ctgaccttcc tcggtctcgt cgttgctggc catggtgcct ccttgagaga
     1621 agtgggcggc gagaggcaac gtcgccgcga gtccgtcaga cctcgagcag ctcttgctcc
     1681 ttgtgcgaca ggacccggtc gatctccccc acgaactcgt gggtgatgcg ctccagctcc
     1741 ttctccgccc gttcgagctc gtccgacgag atgtccccgt ccttctccag ggcctcgagc
     1801 tcgtgccgga ctgcccggcg gctgttgcgg acggcgaccc gcccctcttc ggccttgtgc
     1861 cggaccacct tgaccatctc cttgcggcgt tcctcggtga gcggcgggaa ggccaggcgg
     1921 atcaccaggc cgtcgttgga cgggttgatg cccaggtcgg agttctggat cgccttctcg
     1981 acggccttga tggcgctctt gtcgtacggc gtgacgacca ggaggcgggc ctcggggacg
     2041 ttgaagccgg ccagctgctg aaggggcacc tccgaaccgt agtagtcgac cttcagcttc
     2101 tcgaccaggg ccggagacgc ccgcccggtc cggaccgacg cgaactcgga ctgggtgtgg
     2161 gccacggcct tggccatgtg gtccctggct tcctcgaggg cggcgtcgat gagggagtcg
     2221 tccatcagcg gataagcgta ccgatcgggt cacccaccag ggcgcgccgg atgttgccag
     2281 gctccatcag gtcgaacacg aggatcggca gcgtgttgtc catgcagaag gtgatcgccg
     2341 tcatgtccat cacccgtaga tcctgggcga tgacctcgcc gaaggtgacc tcgtcgaagc
     2401 gcagggccgt cgggtccagc cgggggtcgg ccgagtacac gccgtccact ccggagtggg
     2461 tgcctttgag gatggcctcg gcgtcgatct cgacggcgcg cagggcggcc ggcgtgtctg
     2521 tggtgaagaa cgggctgccc atgccggcgg cgaagatgac gacccggccc ttctccaggt
     2581 gccggatggc ccgccgccgg atgtatggct cggcgatctg cgccatctgg atggcggaca
     2641 ggacccgggt cggctgcccg tggcgctcca gggcgtcctg gagggcgagg gcgtttatga
     2701 cggtgcccag catgcccatg tagtcggagg tggcccggtc catgccggcc accgcgccgg
     2761 tggcgccccg ccagatgttg cccccgccca tcaccacggc gatctcgacg tcgagctccc
     2821 gccgggcctc ggccagctcg acggcgatgc gctcgacggt ctcgccgtcg atcgtctcgt
     2881 ccgacgccgc gctggccagc gcctcgccgg acatcttcag gacgactcgc cgccagcgag
     2941 gcttgcctcc gtccgggccg cgctctccca cccgtcagcc gccgatgacg acctgggcga
     3001 accgcacgac ctgggccccg cccagcacct gctcgacgct ccgcttgtcg tccttggcgt
     3061 agggctgctc gagcagcacg cctccgggga agcgcttgta ccagccgttg agcttcccct
     3121 ccacgatctt ggccagcgcc gcctccggct tgccctcgtt gcgggtctgc acctcgaggg
     3181 aggcccgctc ggcggccacg tcggcggccg ggacctcctc gcgcgccacg tgctggggcc
     3241 gggcgaaggc gatgtggact gcgatgtcgt gggccaggtc gttcgccgcc tcggcgggag
     3301 cgccggccac ctccaccagc accgcgttga ccccgcgccc gctctgcaca tgcaggtagg
     3361 tgtcgagcac gcggtccggg ccggcctcga agcgcacgac gcggcccaag gagatgttct
     3421 ccttgagggt caccttgagg tcgtcgatct ggtcggcgcg ctcggccgcg gcgccctctc
     3481 ccttctcgac caccaggtcg gcgatctcct gcgccagctt ggtgaagtcg ggcgacttgg
     3541 ccacgaagtc ggtctccgac ttgagctcga ccagggccgc cacgcggccc gcggcggaga
     3601 ccgccaccgc gccctgcgcg ttctcccggt cggtgcggtc ggcggccttg cccaggccct
     3661 tctccctcag ccacttggcg gccgcctcgg cgtcgcctgc gttcgcctcc agggcgcgct
     3721 tggcgtccat catccccgcg ccggtggcct ggcgcagggc ctggacgtcc ttcgccgtga
     3781 actctgacat gtctctcatt ctctccgatc gtgctgggtg atggcgggcc gctctaggcc
     3841 tcggcagagt ccgtcgcctc agccggcgcc gggctggcgc catcggcggg cggcgccgcc
     3901 gccaggcggg cctcccgttc ctgggcctga agggcggcct gccgccgggc ctccgcctgg
     3961 tgctgggccc gccgggcctc ctcctccggg tcgatgggcc cggggccggc gctggccgcg
     4021 gccccggggc cgccgccggc tcgggaggcc ttccgggagg cgatgaagcg cccctcgtcg
     4081 accgcgtcgg cgatgatccg gcacatcaac gtgcccgacc ggatggcgtc gtcgttgccg
     4141 gggatcacgt agtcgatcac gtcggggtcg cagttggtgt cgaccaccgc cacgatcggc
     4201 aacccgagct tgttggcctc ggtgacggcg atgtgctcct tcttggtgtc gatgacgaac
     4261 agggcctcgg ggagccgggc catgctccgc aggccgccga ggttgcgctg gagcttgtcg
     4321 agctcgcggg acaggatcag ggcctccttc ttgggcatgg cgtcgaaatc gccggcggcc
     4381 cgcatgcgct cgtactcctg catcttcttc acccgcccgc tgatggtggc gaagttggtg
     4441 agcatcccgc ccagccaacg ctcgttcacg aacggcatgc cgcagcgctg ggcatagtcc
     4501 gcgatggggt cctgggtctg cttcttggtg cccacgaaca ggaccgagcc acccccggcc
     4561 accaggtcgc gggtgaaggt gtaggcgacc tcgatgcgcc gcagcgtctg gttgaggtcg
     4621 atgatgtaga tgccgttgcg ctccccgaag atgaaccgcc gcatcttggg gttccagcgc
     4681 cgggtctggt ggccaaagtg gaccccggct tcgagcagct gcttcatggt gacgacggcc
     4741 acggtgctcc ttcccatcgg ttggtgagta cccggatgct gcgccctcgc ggcccgaggg
     4801 ccgggaaggg cgaccgtggg tgcggccggg caggtcgtga cccggccgag cgccggatca
     4861 gcgttcagtg tagtggccgc ggttgctgcg gggcaccgct caggaaggct gcgcctggcc
     4921 aggcgcgtcc gcagccgggc gccggcggcc gacgacgaga cccgcgacca ccacggaggc
     4981 cagcccgagg gcggccagaa gcccgggcca gcccgtgacc cgcgtgccgg gaccactcgg
     5041 gagcgccgcc cgccggccca gggccctccc ggcgggttcg ccggcggggg cgggcacggt
     5101 gaaggccagg gggacggtgg tgacccggcc gccacccgcg tcggcggtga ccgtcacgcc
     5161 gtagctgccc ggcagcgtcc cggcgaccag ggcctcggtc acggctccgg ccgccacccc
     5221 gtcgtcgcgc ccggccgtgg tcccgtcggg gtcgcggcga gtcagcgcca ccctggcccc
     5281 gggggccacc cggaaggcga ccgtgaccgg ctccccggca tccgggtgcg acggggtgat
     5341 ccgggccgcg gtcaccaccg gcggtccggc ctggtccggc ggcggcacga cccggacctg
     5401 gcccggggcc gggccgggcg agaccgtcca caccccggtc gccgccaccg cgaggggccg
     5461 gcccgctccg tcgagcaccc ggaacgaccc tggggcggtg acggccgccg acgccaggcc
     5521 ggtcgagagg gcgacgcgga ggcgggccgg cagctgctcg ggctggaggg tcaccggacg
     5581 cagcccgccg tagtaggcgg ccaggatgtc ggccgccttc atgccccgca acgccttccc
     5641 gagggcgccg tactggctca tcccgatgcc gtggccccag ccgccgccgt cgattaccgc
     5701 tgtctgggcg gcgccgtcgg tgccgacggt gaagcggtcc gacggcaccg tgacgggcag
     5761 cgcggcgggc ggggtctcct gacggttgag cccgtcgcgg aaggcatc
//
LOCUS       DASWGG010000173         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2338427, whole genome shotgun sequence.
ACCESSION   DASWGG010000173 DASWGG010000000
VERSION     DASWGG010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338427"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VF954_03175"
     CDS             complement(<1..781)
                     /locus_tag="VF954_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9530113.1"
                     /translation="MDYEQIRYEVADSVLTITLDRPDKLNAFTGLMMGEMIDAFDRSD
                     ADDDVRAVIVTGAGRGFCAGADLSGGGGTFDPRAHARGAGGREAIPRDGGGLLTLRIF
                     DSKKPVIAAINGPAVGVGVTMTLPMDVRLAAEEARIGFVFARRGIVPEAGSSWFLPRL
                     VGISQAMEWVATGRVFDAEEALRGRLVRSVHPASELLPAAHALAREIADNAAPVSVAL
                     SRQMLWRMLGADHPMAAHRADLRGVYYRGQSGDAREGISSFL"
     gene            859..1323
                     /locus_tag="VF954_03180"
     CDS             859..1323
                     /locus_tag="VF954_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX9530114.1"
                     /translation="MSAADLHLLARIAVGFALSFVLGFERELRGAAAGDRTFALVGTG
                     AAAVAAVAGRSSPQALGGIVTGVGFIGAGLVFRSPIGRPHGITSAAAVWAVAAIGMVA
                     GLGHPVLAVCLTAVVLVDLELRHVPLLSRLDSRRYAAMVEPDEPRGSEDAGQ"
     gene            complement(1348..>1878)
                     /locus_tag="VF954_03185"
     CDS             complement(1348..>1878)
                     /locus_tag="VF954_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX9530115.1"
                     /translation="DNVEQIFMLTDDELDGFASDPAKFSQTVREREQAYLELFDLEPP
                     FVVAGEPPAVSTWPRRRRPEAEQARPGAVLSGIPGCAGTATGRARIILDPGDPTALEP
                     GDVLVAPVTDPAWTPLFVPAAAVVVDVGAQITHAVIVSRELGIPCVVSVTDATRTIPD
                     GAIVTVDGTAGTVTVH"
BASE COUNT          236 a          691 c          694 g          257 t
ORIGIN      
        1 cgaggaacga gctgatcccc tcccgggcat cccccgactg gccccggtag tagacgccgc
       61 gcaagtccgc cctgtgcgcg gccatgggat ggtcggcgcc gagcatgcgc cacagcatct
      121 ggcgggacag cgccaccgag acgggcgcgg cgttgtcggc gatctcccgg gccagggcgt
      181 gggccgcggg cagcagctcg ctcgccgggt gcacgctgcg cacgaggcgg ccccggagcg
      241 cctcctcggc gtcgaagacc cggcccgtgg ccacccactc catggcctgg ctgatgccca
      301 ccaggcgggg caggaaccag ctcgagcccg cctccggcac gatgccccgc cgggcgaaca
      361 cgaagccgat gcgagcctcc tccgcggcca ggcgcacgtc catcggcagc gtcatggtga
      421 cgcccacgcc cacggcgggc ccgttgatgg ccgcgatgac gggcttcttg gagtcgaaga
      481 tccgcagcgt cagcagcccg ccgccgtccc gcgggatcgc ctcccggcct ccggcacctc
      541 gggcgtgggc ccgggggtcg aacgtcccgc cgccgcccga caggtcggca cccgcgcaga
      601 agccccggcc ggcgccggtc acgatcacgg cgcgcacgtc gtcgtcggcg tcgctgcggt
      661 cgaaggcatc gatcatctcg cccatcatca agccggtgaa ggcgttcagc ttgtccggcc
      721 ggtcgagggt gatggtgagc acggagtcgg ccacctcgta acggatctgc tcgtagtcca
      781 tgtcctcacc tcgtgtgtcg tgcccgccgc gcagggtatc ccgccgcgcg agtgggcggc
      841 ggggccatca tcgttgacat gagcgcggcc gatctccacc tgctcgcccg gatcgccgtg
      901 ggcttcgcgt tgtccttcgt cctcgggttc gagcgcgagc tgcggggcgc ggctgccggc
      961 gaccggacct tcgccctggt gggcaccggc gcggcggcag tggcggccgt cgccggccgg
     1021 tcctcgcccc aggctctcgg cgggatcgtg accggggtgg gcttcatcgg cgccggcctg
     1081 gtgttccgca gccccatcgg caggccccat gggatcacct cggccgcggc cgtgtgggcc
     1141 gtcgccgcca tcggcatggt cgccggcctc ggccatcccg tgctggctgt ctgccttacg
     1201 gccgtggtgc tggtggatct cgagctgcgc cacgtgcccc tgctgtcccg tctggacagc
     1261 cgccgctacg cggccatggt cgagcccgac gaaccccgag gaagcgagga cgccgggcaa
     1321 tgagccgggg aggagtccgc agccgggtca gtggacggtc accgttcccg ccgtcccgtc
     1381 cacggtgacg atggcgccgt cgggaatggt gcgggtggcg tcggtgaccg acaccacgca
     1441 cgggatgccc agctcccgcg agacgatgac ggcgtgggtg atctgggcgc ccacgtcgac
     1501 caccacggcc gccgccggga cgaacagcgg cgtccacgcc gggtcggtga ccggcgcgac
     1561 gagcacgtcg cccggctcga gcgcggtcgg gtcgcccgga tcgaggatga tgcgggcccg
     1621 ccccgtcgcg gtgccggcgc acccggggat accggacagg acggcgcccg ggcgggcctg
     1681 ctccgcctcc ggacgccggc ggcggggcca ggtgctgacg gccgggggct ctccggccac
     1741 cacgaacggc ggctccaggt cgaacagctc gaggtaggcc tgctcccgct cacggacggt
     1801 ctgggagaac ttcgccgggt cggaggcgaa gccgtcgagc tcgtcgtcgg tgagcatgaa
     1861 gatctgctcg acgttgtc
//
LOCUS       DASWGG010000174         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_816270, whole genome shotgun sequence.
ACCESSION   DASWGG010000174 DASWGG010000000
VERSION     DASWGG010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816270"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..740
                     /locus_tag="VF954_03190"
     CDS             72..740
                     /locus_tag="VF954_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX9530116.1"
                     /translation="MSDQSPEALLTVTDEARQHISSLREGEDGGDKLGLWVEVSGVEA
                     GAYRYDMWFEELADAAPSDAVVHHDDLAVVVPATSVERLRGATLDLVGDGMVIRNPNA
                     PPAVASASAGAGAGADGVAGADMPQGDLSGEVAQAVLRVLEESVNPGIAAHGGRAELV
                     AVEDGAAFLRLLGGCVGCGLATVTLSQGIEVAIRDAVPDIVRVVDVTDHASGTNPYYE
                     PAKK"
     gene            799..1488
                     /locus_tag="VF954_03195"
     CDS             799..1488
                     /locus_tag="VF954_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9530117.1"
                     /translation="MELVQERGTPIGRRVILAMLGLGGAGVLFGSAAQRVLARLGTPA
                     FLPGSNRFRIYSVVGYLPERSAGAYRLAVTGLVDNPLTLSFADLQAMPATRMVKDFQC
                     VTGWRVTGVTWTGVKLADVLDAAHVKAEGHAITFKSFDGEYTESLTMEQARRDDVIVA
                     YAMENGPVSAEHGGPVRMYVAPMYGYKSTKWLQEIEVVRAVDPGYWEVRGYDVDGWVG
                     KSNGRGDRPTS"
     gene            1541..2170
                     /locus_tag="VF954_03200"
     CDS             1541..2170
                     /locus_tag="VF954_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX9530118.1"
                     /translation="MRFDPTERVVHWSNATLFLILIATGASLYVPSLSALVGRRELVR
                     NVHVYAGLALPLPVLLGAAGRRWGSALRGDLARINRWSSDDVRWFRSLSRDPMAELGK
                     FNPGQKLNAAFTGGAILVMLGTGSILHWYKPFPLGWRTGATFVHDWVAVLLFCTISGH
                     ILFAFSDREALGAMVGGRVSARWAARHAPRWRAEVEAAAEAGRAEAAQS"
BASE COUNT          303 a          744 c          813 g          315 t
ORIGIN      
        1 aagtcgggcg cccggtacgt cgaggtgggc agcaggatca gtagacgggc cacccgccta
       61 tcatggtcgc catgagcgac cagtcgcccg aggccctgtt gaccgtcacg gacgaggccc
      121 gccagcacat ctcgtccctc cgagagggcg aggatggcgg cgacaagctc ggcctgtggg
      181 tggaggtgag tggcgtggag gcgggcgcct accgctacga catgtggttc gaggagctgg
      241 ccgacgctgc ccccagcgac gcggtcgtcc accacgacga cctcgcggtc gtggtcccgg
      301 ccacgagcgt cgagcggctg aggggggcca ctctcgacct ggtcggcgac ggcatggtca
      361 tccgcaaccc caacgccccg ccggcggtgg cctccgcatc ggcgggggcg ggcgccgggg
      421 ccgatggggt ggccggggcc gacatgccgc agggcgatct ctccggtgag gtggcccagg
      481 ccgtgctgcg ggtgctcgag gaatcggtca accctggtat cgccgcccac ggcggccggg
      541 ccgagctggt cgcggtcgag gacggtgcgg ccttcctgcg cctcctgggc gggtgcgtcg
      601 ggtgcggcct ggccacggtc accctgtcgc agggcatcga ggtggccatc cgcgacgccg
      661 tgcccgacat cgtccgcgtg gtggacgtca ccgaccacgc cagcggcacc aacccctact
      721 acgagccggc caagaagtaa gcgatccgta aggcggcgcg acggtccata gcggtccggg
      781 cgccataccc tcgcggtgat ggagctcgtc caggagcggg gcacacccat cgggcggcgc
      841 gtcatcttgg ccatgctggg gctcgggggc gcgggcgtgc tcttcggctc cgccgcccag
      901 cgggtcctcg cccggctcgg caccccggcg ttcctgcccg gcagcaaccg cttccggatc
      961 tactcggtgg tgggctacct ccccgagcgc agcgccggcg cctaccggct ggcggtgacc
     1021 ggtctggtcg acaacccgct cacgctgagc ttcgccgacc ttcaggccat gccggcgacc
     1081 cggatggtga aggacttcca gtgcgtcacg ggctggcggg tcaccggggt gacgtggacc
     1141 ggggtcaagc tggccgacgt gctggacgcg gcccacgtca aggccgaggg ccatgcgatc
     1201 accttcaagt cgttcgacgg ggagtacacc gagagcctca ccatggagca ggcccggcgc
     1261 gacgacgtga tcgtggccta cgccatggag aacggaccgg tcagcgccga gcacggcggc
     1321 ccggtccgca tgtacgtcgc ccccatgtac ggctacaagt ccaccaagtg gctccaggag
     1381 atcgaggtgg tgcgggccgt cgatccgggc tactgggagg tccggggcta cgacgtcgac
     1441 ggatgggtcg ggaagtcgaa tggccgcggt gaccggccca catcctgacc cggtcgcacc
     1501 gagcggtgcg gcacagaggc aaggcgcggc ccggcggctg gtgcgcttcg atccgacgga
     1561 gcgggtggtg cactggtcca acgcgaccct cttcctgatc ctgatcgcga ccggggcctc
     1621 cctctatgtc ccgtcgctgt ccgcgttggt gggccggcgc gagctggtgc gaaacgtgca
     1681 tgtatacgcg ggcctggccc tgccgctgcc cgtcctgctg ggcgccgcgg ggcgacggtg
     1741 gggctcggcc ctgcggggcg atctggcccg catcaaccgc tggagcagtg acgacgtgcg
     1801 gtggttccgc tcactcagcc gcgacccgat ggcggagctc ggcaagttca accccggcca
     1861 gaagctcaac gccgccttca ccggcggcgc catcctcgtc atgctgggga cgggctcgat
     1921 ccttcactgg tacaagccgt tcccgctcgg gtggaggacg ggggccacgt tcgtccacga
     1981 ctgggtcgcg gtgctcctgt tctgcacgat cagcgggcac atcctgttcg ccttctcgga
     2041 ccgcgaggcg ctcggggcga tggtgggcgg gcgggtcagc gccagatggg cggcgcgcca
     2101 cgcaccacga tggcgggccg aggtggaagc cgcagccgag gccggccggg ccgaggcggc
     2161 tcagtcgtag acgag
//
LOCUS       DASWGG010000175         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_82537, whole genome shotgun sequence.
ACCESSION   DASWGG010000175 DASWGG010000000
VERSION     DASWGG010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82537"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF954_03205"
     CDS             complement(<1..252)
                     /locus_tag="VF954_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530119.1"
                     /translation="MKACCAAGYQSDAVALILGESYHPGGTALTRRLAQALGLRPGER
                     IADVASGPGTTALLLAAEFGVDVDGIDLAAASVAAANARA"
     gene            complement(309..1883)
                     /locus_tag="VF954_03210"
     CDS             complement(309..1883)
                     /locus_tag="VF954_03210"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9530120.1"
                     /translation="MTIAQKDLSTSRKKVDRDEVFLEYTKSICPVCKIVTDAQVNIRD
                     NKVFLRKRCPTHGPFEALLYSDAQMYMDSLRFNKPGTIPLETQTEVVDGCPLDCGLCP
                     EHKQHACLGIIEVNTGCNLDCPICFADSGHQPDGFSLTLDQVEAGLDAFVRSEGEPEV
                     VMFSGGEPTVHPQILDFLRLAADKGVRHLNLNTNGLRLAHDRKFMAELAALEKRPNIY
                     LQFDGLTEATHLAFRGRDLRAAKAAALDACAEHGLTVTLVAAVERDLNEGELGDIVRF
                     GIAHPAVRSVAFQPVTHSGRHVSFDPLTRLTNADIVHGLVEQCPDWFKASDFFPVPCC
                     FPTCRSITYLLVDGDTVVPIPRLLDIEEHLDYVTNRVVPDLDIRHALEKLWSASAVPG
                     TPTTAAQLECATCGIDLPEALKSLTDKAFMIVIQDFQDPYTLNVKQLMKCCVEEITPD
                     GRLIPFCAYNSVGYREQVREQLSGVAVPTIVPNAGELAPVLLTTRYGSKTAPAANSAG
                     RPVSGRDATNIGGTLR"
     gene            complement(2000..>2739)
                     /locus_tag="VF954_03215"
     CDS             complement(2000..>2739)
                     /locus_tag="VF954_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9530121.1"
                     /translation="RPPRPSPPGALMGVLDRAACPVEADSRAFTDAALAELRAGGWSP
                     AGWARFVAGVAVRSGEQVVAHPRPAAELSLLHGVFLVAARRRGRWWITMSWLMAITHL
                     GLLGRRRSIGWPNAISLTRANLAVTGEPLGRWLGVVAAASDKLDGTLARRQGPTMFGF
                     YADSLADAAFWTWLGARRDPSRLVLAASVAAWAAPVAAMVAASITKGELVESPRPALP
                     RPAAAMQVVLAVRHLRTRCPARAGPTR"
BASE COUNT          393 a          875 c         1064 g          407 t
ORIGIN      
        1 cgcccgggca ttggccgcgg cgacgctggc cgcggccagg tcgatcccgt cgacgtcgac
       61 gccgaactcg gccgccagga gcaaggcggt ggtgccggga ccgctggcga cgtcggcgat
      121 gcgttcgccg ggccgcagcc cgagcgcctg ggcgaggcgg cgggtgaggg cggtcccgcc
      181 cgggtggtag ctctcaccca ggatgagggc gacggcgtcg ctctggtagc cggccgcgca
      241 gcaggccttc acctcctccg gcgatgggcc cgacgtcgcc cacccagacg gcgccggtcg
      301 cgactcgctc atcgcagcgt gccgccgatg ttggtggcgt cgcgaccgga cacggggcga
      361 cccgcagagt tggcggcggg cgcggtcttt gagccgtagc gggtggtgag cagcaccggc
      421 gcgagctcgc cggcgttggg gacgatggtc ggcaccgcca ccccggagag ctgctcgcgc
      481 acctgctcgc ggtagccgac ggagttgtag gcgcagaaag ggatcagccg cccgtcgggg
      541 gtgatctcct ccacgcagca tttcatgagc tgcttgacat tcagggtgta ggggtcctgg
      601 aagtcctgga tgacgatcat gaacgccttg tcggtcagcg acttcaaggc ctcgggcagg
      661 tcgatgccgc aggtggcgca ctccagctgg gcggccgtgg tgggtgtgcc ggggacggcc
      721 gaggcgctcc acagcttctc cagggcgtgg cggatgtcga ggtcgggcac cacccggttg
      781 gtgacgtagt cgaggtgctc ttcgatgtcg aggaggcggg ggatgggcac gacggtgtcg
      841 ccgtcgacga gcaggtaggt gatcgaccgg caggtcggga agcagcacgg gaccgggaag
      901 aagtcagacg ccttgaacca gtcggggcac tgctcgacga ggccgtggac gatgtccgcg
      961 ttggtgagcc gggtgagcgg gtcgaagctg acgtgacggc cggagtgggt gacgggctgg
     1021 aaggcaaccg agcggacggc cgggtgggcg atgccgaagc ggacgatgtc gcccagctcc
     1081 ccctcgttga ggtcgcgctc gacggcggcg accaacgtga cggtgaggcc gtgctcggcg
     1141 caggcgtcta gcgcggcggc cttggcggcg cgcaggtccc ggccccggaa ggcgagatgg
     1201 gtggcctcgg tcagcccgtc gaactgcagg tagatgttgg gccgcttttc gagggcggcc
     1261 agctcggcca tgaacttgcg gtcatgggcc aggcgcagac cgttggtgtt caagttgagg
     1321 tggcgcacgc ctttgtcggc ggccaaccgc aggaagtcga ggatctgggg gtggacggtc
     1381 ggctcgccgc ccgagaacat gaccacctcc ggctcgccct cggagcggac gaaggcgtcc
     1441 agtccggctt cgacttggtc gagggtgagc gagaagccgt cgggctggtg gccggagtcg
     1501 gcgaagcaga tcgggcagtc gaggttgcag ccggtgttga cctcgatgat gcccagacag
     1561 gcgtgctgct tgtgctcggg gcacagcccg cagtcgagcg ggcagccgtc gacgacctcg
     1621 gtctgggttt ccagcgggat ggtgccgggt ttgttgaacc gcagcgagtc catgtacatc
     1681 tgggcgtcgg agtacagcag cgcctcgaag ggcccgtggg tcgggcagcg cttgcgcagg
     1741 aacaccttgt tgtcgcgaat gttgacctgg gcgtcggtta cgatcttgca caccgggcag
     1801 atgctcttgg tgtactcgag gaacacctcg tcccgatcaa ccttcttccg gctggtcgat
     1861 aggtcctttt gggcgatggt cacaacggct tcctcctcgg cgtggggtgg tggccgccgg
     1921 gcggggcgcg ggcggggcgc cccatccggc ccacgaaacc cggtgcggcg accggcctat
     1981 tccaggtatt cgtcgatctt tatcgggttg gccctgctcg tgcgggacat cgggtgcgta
     2041 ggtgccgcac ggccaggacg acctgcatgg ccgccgcggg gcgcggcagt gcgggtcggg
     2101 ggctttcgac cagctcgcct ttggtgatgc tggccgccac catcgccgcg accggcgcgg
     2161 cccacgcagc cacacttgcg gccagcacga gacggctcgg gtcgcgccgg gcgcccagcc
     2221 acgtccagaa ggcggcgtcg gcgagggagt cggcgtagaa gccgaacatg gtcggcccct
     2281 ggcggcgggc cagggttcca tccagcttgt ccgacgctgc ggcgaccacg ccgagccacc
     2341 ggccgagcgg ctcgccggtg acggccaggt tggcccgggt caggctgatg gcgttcggcc
     2401 agccgatcga ccgtcgccgg cccagcaacc ccagatgggt gatagccatc agccagctca
     2461 tggtgatcca ccaccggccc cgacgtcgcg ccgcgacaag gaacacgccg tggaggaggc
     2521 tgagctcggc cgcgggccgg ggatgggcga ccacctgctc gccggaacgc accgcgaccc
     2581 cggccacgaa gcgagcccat ccggccggcg accagccgcc agcccgcagc tcggccaagg
     2641 cggcgtcggt gaaggcccgg ctgtccgcct cgacggggca ggcggcccgg tccagcactc
     2701 ccatcagcgc cccgggtggg ctcgggcggg gcgggcggg
//
LOCUS       DASWGG010000176         5626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2751048, whole genome shotgun sequence.
ACCESSION   DASWGG010000176 DASWGG010000000
VERSION     DASWGG010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2751048"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VF954_03220"
     CDS             <1..836
                     /locus_tag="VF954_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEX9530122.1"
                     /translation="GSAANTAAGVASLGARAGFVGLVRDDLLGGVYAHDLRAAGVAFD
                     VPPAPDGPATGRSLILVTPDAERTMNTFLGAASLLGEAHIDPVTVGRAAVLYLEGYLW
                     DSPSGQRAYRKAIAAARRGGRRVALSLSDPFCVERHREGFRDLIDGGVDLLFGNELEV
                     GLLLGVTDAEDAVAHLQRPGLVTAVTLGGAGSMVVTGDEVVAVPAHPVEHVVDTTGAG
                     DLYAAGFLAGLARGLSWRDCARLGGLAAAEIISHLGARPLASLEKLAVAAGLLPLTPS
                     R"
     gene            833..1735
                     /locus_tag="VF954_03225"
     CDS             833..1735
                     /locus_tag="VF954_03225"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX9530123.1"
                     /translation="MTSRWLERRVLAYAHQGGARQAPSSTLMAMEQALAAGATGLELD
                     VHGTADGHLVVCHDATVDRTTDGTGAIAGLTLAQLQRLDNAYWWAPGEQAVQGRPPGE
                     YPLRGRAPTDPDLGIATLRSVLERFPGVILNLDIKQTAPAVRPYEADLAAVLREHGRV
                     DDVIVASFDDRATDAFSAAAPEIATSAGAMATALFCRAVAVGEQPPAMRHAALQVPAS
                     YSGVELVTREVVAAAHRAGVAVHVWTVDDPAEMERLLDLGVDGIISDVPSVLVGALRK
                     RGVAWPGPAGSTLGQPRPQLGFLP"
     gene            1824..2900
                     /gene="ychF"
                     /locus_tag="VF954_03230"
     CDS             1824..2900
                     /gene="ychF"
                     /locus_tag="VF954_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX9530124.1"
                     /translation="MERLGIVGLPNAGKSALFNALTGGSAAVAPHPFSTTETHVGVAH
                     VPDHRLGALAEMSKSRKVVQATVELVDIAGLVAGAASGEGLGNRFLAGIREADALVLV
                     LRAFEDANVVGGHDPVEDLGVLELELVLADAATVENQLDKRRKASRTDKSLQAGVDAM
                     AEALTALHGGSPIYRSGLGREARQHLRPAFLLTDKPVLAVVNLGEDQVGDADALVKAV
                     DEELGGVADVLGVSVQLEAEAAQLDPSERAELLEGLGLGEGAFPRVVRAAYHLLGRRT
                     FLTTGDKESRAWTFRAGARAPECAGVIHSDLQRGFIRAEVIRWDELIELGSWARAKEV
                     GRLRVEGKDYEVVDGDVLEIRFNV"
     gene            2924..3241
                     /locus_tag="VF954_03235"
     CDS             2924..3241
                     /locus_tag="VF954_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX9530125.1"
                     /translation="MREGEVLATVEVGETWGARLRGLLGRDELEGALLIRPARSVHTF
                     GMRFPIDVAYCSRQLVVVDCACMRPHRIGRPRPKAHCVIEARAGAFERWGLGPGDQLE
                     IKG"
     gene            3238..4086
                     /gene="rsmI"
                     /locus_tag="VF954_03240"
     CDS             3238..4086
                     /gene="rsmI"
                     /locus_tag="VF954_03240"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX9530126.1"
                     /translation="MTGAVGVLVLVATPIGNLGDLSPRAVTTLGASDVIACEDTRHTR
                     KLLSHAGIHGPAVIAVHGHNEAAQLGRVLRLLDQGRRVALVSDAGMPGISDPGSRLVA
                     GVAAAGIRIEVVPGPSAAIAALVVSGLPTDRWCFEGFLPVRGRDRRDRLEAVRGETRT
                     TVLYESPHHLAVTLQDLCDACGPRRRLAIARELTKLHEEVWRGTLEGALARVSGTEPR
                     GEHVLVLAGAPPSQPATDDEVEASLRARLGAGLGARAAVAETAAELGVPKRRAYDIAL
                     RLQGKA"
     gene            complement(4069..4365)
                     /locus_tag="VF954_03245"
     CDS             complement(4069..4365)
                     /locus_tag="VF954_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9530127.1"
                     /translation="MDSHRGVARRIDHLGRIVVPAELRRALGIEPGDELEVATDGPAI
                     TLTKVETRCVFCGAERALRPFRAKLVCGRCASELGESPGPDGGEPGGAGSGLAL"
     gene            4402..>5626
                     /gene="metG"
                     /locus_tag="VF954_03250"
     CDS             4402..>5626
                     /gene="metG"
                     /locus_tag="VF954_03250"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX9530128.1"
                     /translation="MARFFVTTPIYYVNDAPHIGTAYTTVTADGAARWHRLLGDEVFF
                     LTGTDEHGLKVAQTAEANGTTPQDWTDRTSERFREAWKLLDISYDDFIRTTEPRHHAS
                     VQTFLGRIYDRGDIELGTYEGPYCVACEAYYRVDELVDGNCPIHGRPVQLMSEQNYFF
                     KLSRYQDRLLEWYASHPDAVRPESKRNEALGFIRGGLEDISITRTSLSWGVPVPWDDR
                     HVFYVWYDALVNYVTAIGFGTDASSFAAWWPAVHHLIGKDILRFHCVWWPAMCMAAGI
                     DPPAHISVHGFLLVGGEKMSKTRLNQIAPEGLVSDFGVDGFRYHFLRDQAFGPDGEFS
                     YEAMVERYNADLANNLGNLLSRVATVVDKKCGGVGPAPRAGSPLAAVVAPAYEAAASA
                     WARVAPNEALDATWRI"
BASE COUNT          711 a         2072 c         2056 g          787 t
ORIGIN      
        1 gcggctcggc ggccaacacc gccgcgggcg tggcgtccct cggcgcccgg gccgggttcg
       61 tcgggctggt gcgcgacgac ctcctcggcg gggtctacgc ccacgacctc cgggcggccg
      121 gcgtcgcctt cgacgtgccg ccggccccgg acggcccggc gacggggcgc agcctgatcc
      181 tcgtcacgcc ggacgcggag cgcaccatga acaccttcct cggcgccgcc tctctcctgg
      241 gtgaagccca catcgacccg gtgacggtcg ggcgggccgc ggtgctgtac ctcgagggct
      301 acctgtggga ctcgcccagc gggcagcggg cctaccgcaa ggccatcgcc gcggcccgcc
      361 gcggcggccg cagggtcgcc ctgtcgttgt ccgatccctt ctgcgttgag cggcaccgcg
      421 aagggttccg ggacttgatc gacggcggcg tcgacctgct gttcggcaac gagctcgagg
      481 tcggcctgct cctcggcgtg acggacgccg aggacgccgt ggcccacctc cagcgccctg
      541 gcctggtgac ggcggtgacc ctgggcggcg ccggctcgat ggtcgtcacc ggcgacgagg
      601 tggtcgcggt gccggcccat cccgtcgagc acgtggtgga cacgaccggc gcgggtgatc
      661 tgtacgcggc cggctttctc gccggactgg cccgcgggct gtcctggcga gactgcgccc
      721 gcctcggcgg cctcgccgcg gccgagatca tctcccacct cggggcccga cccctcgcgt
      781 cgctcgagaa gctggcggtg gcggccgggc tgctgccgct cacccccagc cgatgaccag
      841 ccggtggctg gagcggcgcg tcctggccta cgcccaccag ggcggggcgc gccaggcgcc
      901 gtcgagcacg ctgatggcca tggagcaggc cctggcggcg ggcgctaccg gcctcgagct
      961 ggatgtccac ggcacggcgg acggccatct ggtggtctgc cacgacgcca cggtcgatcg
     1021 caccaccgac gggacgggcg ccatcgccgg gttgacgctg gcgcagctcc agcggctcga
     1081 caacgcctac tggtgggcgc ccggggagca ggcggtgcag ggccgcccgc cgggcgagta
     1141 cccgctgcgt ggccgggcgc ccaccgaccc cgacctcggc atcgccaccc tgcgctcggt
     1201 cctcgagcgg ttcccgggcg tgatcctcaa cctggacatc aagcagacgg cgccggccgt
     1261 ccgcccgtac gaggcggacc tggccgcggt gctgcgcgag cacgggcggg tcgacgacgt
     1321 gatcgtggcg tccttcgacg accgtgcgac cgacgccttc tccgccgccg cgcccgagat
     1381 cgccacctct gccggcgcga tggccacggc gctgttctgc cgggcggtgg ccgtcgggga
     1441 gcagccgcct gccatgcgcc atgccgccct ccaggtcccg gccagctact cgggcgtgga
     1501 gctggtcacc cgcgaggtgg tcgccgccgc ccaccgggcg ggcgtggccg tgcacgtctg
     1561 gaccgtcgac gaccccgccg agatggagcg cctgctcgac ctgggtgtgg acggcatcat
     1621 ctccgacgtg ccgtccgtgc tggtgggcgc tctccggaaa cggggggtcg cctggcccgg
     1681 ccccgccggc tcaacccttg gtcagccccg cccgcagttg ggcttcttgc cgtgattggc
     1741 cttcttcttg gaccgcaact tccgcttgac cgtccgcttc gacatagggg ggcgagggta
     1801 ccgggggcgg tagcctcggg ccaatggagc gacttggcat cgtcggtctc cccaacgcgg
     1861 gtaagtcggc cctgttcaac gccctgaccg gcggatctgc cgccgtggcg ccccacccct
     1921 tctccaccac ggagacccat gtcggggtgg cccacgtgcc cgaccaccgg ttgggcgccc
     1981 tggccgagat gtcgaagagc cgcaaggtcg tgcaggcgac cgtggagctg gtggacatcg
     2041 ccgggctggt ggccggggcc gcctccggcg aggggctcgg caaccgcttc ctggccggta
     2101 tccgcgaggc cgacgccctc gtgctcgtcc tccgggcctt cgaggacgcc aacgtcgtcg
     2161 gcggccacga ccccgtcgag gacctcgggg tgctcgagct ggagctggtg ctggccgacg
     2221 cggccaccgt cgagaaccag ctcgacaagc ggcgcaaggc ctccaggacg gacaagtccc
     2281 tccaggccgg ggtggacgcc atggccgaag ccctcaccgc gctccacggc gggtccccga
     2341 tctaccggtc gggcctgggc cgggaggcca ggcagcacct gaggcccgcc ttcctgctca
     2401 ccgacaagcc cgtgctcgcc gtcgtgaacc tcggcgagga ccaggtgggc gacgccgacg
     2461 cgctggtcaa ggcggtcgac gaggagctgg gcggggtggc cgatgtcctg ggggtgagcg
     2521 tgcagctgga ggccgaggcc gcccagctcg acccgtcgga gcgggccgag ctgctcgagg
     2581 gcctgggcct gggcgaaggg gcgttccccc gggtcgtgcg ggccgcctac cacctcctcg
     2641 gccggcgcac gttcctcacg acaggggaca aggagtcgcg ggcgtggacc ttccgggcgg
     2701 gcgccagggc gccggaatgc gccggcgtca tccactccga cctgcagcgg ggcttcatcc
     2761 gggcggaggt catccggtgg gacgagctga tcgagctggg ttcgtgggcc agggccaagg
     2821 aggtcggacg gctccgggtc gagggcaagg actacgaggt cgtcgatggc gacgtgctcg
     2881 agatccgctt caacgtctga cggttgctgc catggcctgg ctgatgcgag aaggtgaggt
     2941 gctggccacc gtcgaggtgg gcgagacctg gggcgcccgg ctcagggggc tgctcggccg
     3001 ggacgagctc gagggcgccc tgctgatccg gccggcgcgg tcggtgcaca ccttcgggat
     3061 gcggttcccg atcgacgtgg cctactgctc gcgccagctc gtcgtcgtcg actgcgcctg
     3121 catgcggccc caccggatcg gccggccccg accgaaggcc cactgcgtga tcgaggcccg
     3181 ggctggtgcc ttcgagcgtt ggggcctggg cccgggcgac cagctcgaga tcaaaggatg
     3241 acgggcgcgg tcggggtgct agtgctggtg gccactccga tcggaaatct gggcgacctg
     3301 tcgccccgcg ccgtcaccac gctcggggcg tcggacgtga tcgcgtgcga ggacacccgc
     3361 cacacccgca agctgctgtc ccacgccggc atccacgggc cggccgtgat cgccgtccac
     3421 ggccacaacg aggccgccca gctcggtcgc gtgctgcggc tgctcgatca gggccggcgg
     3481 gtggcgctgg tctccgacgc cggcatgccc ggcatctcgg acccgggcag ccggctggtg
     3541 gccggggtgg ccgcggccgg cattcggatc gaggtggtgc ccgggcccag cgccgccatc
     3601 gccgccctgg tggtgagcgg gctgccgacc gaccggtggt gcttcgaggg gttcctgccc
     3661 gtgaggggcc gggaccggcg cgaccggctg gaagccgttc gcggcgagac ccgcacgacc
     3721 gtgctgtacg agtcgcccca ccacctggcc gtcaccctcc aggacctgtg cgacgcgtgc
     3781 gggccgcggc ggcgcctcgc catcgcccgg gagctgacca agctgcacga ggaggtgtgg
     3841 cgcggcaccc tcgagggggc gctggcgcgg gtgtcgggca ccgagcctcg aggtgagcac
     3901 gtgttggtgc tggccggggc gccgcccagc cagcccgcca ccgacgacga ggtcgaggcc
     3961 tcccttcggg cccgcctcgg cgccggcctg ggcgccaggg cggccgtggc cgagacagcc
     4021 gccgagctgg gcgttccgaa gcgccgggcc tacgacatcg ccctgcgcct acagggcaag
     4081 gcctgagccc gccccacccg gctcgccgcc gtccggtccg gggctttctc ccagctccga
     4141 cgcgcaccgc ccgcacacca gcttggccct gaagggccgc agcgcccgct ccgccccgca
     4201 gaacacgcac cgggtctcca ccttggtcag ggtgatcgcc ggcccgtcgg tggcgacctc
     4261 gagctcgtcg cccggctcga tgcccagggc ccgccgcagc tccgccggga ccacgatgcg
     4321 gcccaggtgg tcgatgcggc gggccacgcc ccggtgcgaa tccatgtccg aacgccctct
     4381 ctccgtcggt actgtacgca ggtggcccgc ttcttcgtga cgacgccgat ctactacgtg
     4441 aacgacgcgc cgcacatcgg caccgcctac accaccgtca cggccgacgg cgcggcccgg
     4501 tggcaccggc tgttgggcga tgaggtgttc ttcctgaccg gcaccgacga gcacgggctg
     4561 aaggtggccc agacggccga ggccaatggc accaccccgc aggactggac cgaccgcacg
     4621 agcgagcgct tccgcgaggc ctggaagctg ctcgacatct cctacgacga cttcatccgc
     4681 acgaccgagc cccggcacca cgcctcggtc cagacgttcc tgggccgcat ctacgaccgc
     4741 ggcgacatcg agctggggac ctacgagggg ccctactgcg tggcctgcga ggcctactac
     4801 cgggtggacg agctggtcga cgggaactgc ccgatccacg gccgccccgt gcagctcatg
     4861 agcgagcaga actacttctt caagctgtcc cggtaccagg accgcctgct ggagtggtac
     4921 gcctcccacc ccgacgccgt gcggccggag agcaagcgca acgaggccct gggcttcatc
     4981 cggggcgggc tggaggacat ctccatcacc cgcacctcgc tctcgtgggg cgtcccggtg
     5041 ccctgggacg accggcacgt cttctacgtc tggtacgacg cgctcgtcaa ctacgtcacc
     5101 gccatcggct tcggcaccga cgcctcgtcg ttcgcggcgt ggtggccggc ggtgcaccac
     5161 ctgatcggca aggacatcct gcggttccac tgcgtgtggt ggccggcgat gtgcatggcc
     5221 gcgggcatcg acccgcccgc ccacatctcc gtgcacggct tcctgctggt cggcggggag
     5281 aagatgtcca agacccggct caaccagatc gcgccggagg gcctggtctc ggacttcggc
     5341 gtcgacgggt tccggtacca cttcctccgc gaccaggcgt tcggtccgga cggggagttc
     5401 tcctacgagg ccatggtgga gcgctacaac gccgacctgg ccaacaacct cggcaacctg
     5461 ctgtcccggg tggcgaccgt ggtggacaag aagtgcgggg gcgtggggcc cgcgccccgg
     5521 gccggctcgc cgctggccgc ggtcgtggcg ccggcctacg aggccgccgc ctcggcgtgg
     5581 gcgcgggtgg ctcccaacga ggcgctcgac gccacgtggc gcatca
//
LOCUS       DASWGG010000177         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1148099, whole genome shotgun sequence.
ACCESSION   DASWGG010000177 DASWGG010000000
VERSION     DASWGG010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148099"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1514
                     /locus_tag="VF954_03255"
     CDS             <1..>1514
                     /locus_tag="VF954_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX9530129.1"
                     /translation="IGQLLVEDGAVTDAVLMGALARQLGLEFVDLAEYPIDRGAVALL
                     PEAMARRLLALPITWADDRLQVAMADPGNVLAIDDLRAVTGAEIQVVVATRTQLAEAI
                     DRFHRLDAEVDEVAQIAADELAEDDDADVAREAVEDAPIVKFVNLLIAQAVADRASDI
                     HVEPTEHDLRIRFRVDGVLHEVMHSPRSIQGGVISRLKVMADINIAERRIPQDGRISL
                     NVAGKPVDLRVATLPTVYGEKVVMRILDKAQARLSLPDLGFHPAVLPRFEACFRKPYG
                     TVLVTGPTGSGKSTTLYATLNVLNSTDKNVITVEDPVEYRLPGVNQVQVNPKAGLTFA
                     SALRSILRSDPDIVLVGEIRDKETATIAIEASLTGHLVLSTLHTNDAASTPMRLVEMG
                     VEPFLVVSSLDCVVAQRLARKLCDRCREEYEPTHGELTEAGWPAALLKAEGAPTLYRA
                     VGCAACSRTGYRGRLAVHEVMVMSEELRRMVVERYSSDDIKKTALAQGMLTLRE"
BASE COUNT          227 a          582 c          490 g          215 t
ORIGIN      
        1 atcggccagc tgctggtgga ggacggcgcc gtcaccgacg ccgtcctcat gggcgccctg
       61 gcccgccagc tcggcctcga attcgtcgac ctggccgagt accccatcga ccggggcgcg
      121 gtggccctgc tccccgaggc catggcccgc cggctcctgg ccctgccgat cacctgggcc
      181 gacgaccggc tccaggtggc catggccgac cccggcaacg tgctggccat cgatgacctc
      241 cgggccgtca ccggggccga gatccaggtg gtggtggcca cccgcaccca gctcgccgag
      301 gccatcgacc ggttccaccg cctcgacgcc gaggtcgacg aggtggccca gatcgccgcc
      361 gacgaactgg ccgaggacga cgacgccgac gtcgcccggg aggccgtcga agacgcccct
      421 atcgtcaagt tcgtcaacct cctcatcgcc caggcggtgg ccgaccgggc gtccgacatc
      481 cacgtcgagc ccaccgagca cgacctgcgg atccggttcc gggtcgacgg tgtgctccac
      541 gaggtcatgc actcgccccg gtccatccag ggcggtgtca tcagccggct caaggtcatg
      601 gccgacatca acatcgccga gcgccgcatc ccccaggacg gtcgcatctc cctcaacgtc
      661 gccggcaagc ccgtcgacct ccgggtggcg acgctgccga ccgtctacgg cgagaaggtc
      721 gtcatgcgga tcctggacaa ggcccaggct cgcctgtccc tccccgacct cgggttccac
      781 ccggcggtgc tgccccgctt cgaggcctgt ttccgcaagc cctacggcac cgtcctcgtc
      841 accgggccca ccgggtcggg caagtcgacg acgctctacg ccaccctcaa cgtcctcaac
      901 tcgaccgaca agaacgtcat caccgtcgag gacccggtcg agtaccgcct gcccggcgtc
      961 aaccaggtgc aggtcaaccc caaggccggg ctgaccttcg cctcggccct gcggtcgatc
     1021 ctgcggtccg atcccgacat cgtcctcgtc ggcgagatcc gggacaagga gacggccacc
     1081 atcgccatcg aggcgtcgct caccggccac ctggtgctgt cgacgctgca caccaacgac
     1141 gccgcgtcta cccccatgcg gctggtggag atgggcgtcg agccgttcct ggtcgtgtcg
     1201 tcgctcgact gtgtggtggc ccagcgcctg gcccgcaagc tgtgcgaccg ctgccgggag
     1261 gagtacgagc cgacccacgg cgaactcacc gaggccggct ggccggccgc cctcctcaag
     1321 gccgagggcg cgccgacgct gtaccgggcg gtgggctgcg ccgcctgctc ccgcaccggc
     1381 taccggggcc gcctcgccgt ccacgaggtc atggtgatga gcgaggagct ccgccgcatg
     1441 gtggtggagc gctactcgtc ggacgacatc aagaagaccg cgttggccca ggggatgctg
     1501 accctccggg agga
//
LOCUS       DASWGG010000178         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2751922, whole genome shotgun sequence.
ACCESSION   DASWGG010000178 DASWGG010000000
VERSION     DASWGG010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2751922"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2488)
                     /locus_tag="VF954_03260"
     CDS             complement(<1..2488)
                     /locus_tag="VF954_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9530130.1"
                     /translation="MGYGTDRRRLGWLEAGTAVTAAAVLAAAALTASRGPDPVALALF
                     AGLLVWAENVAVRLPTSTRVSPSFMVAMATVTAYRGGGALLGVALTGACGGLAIDHLR
                     RRRYRIVVFNCAQYLLAAAAAEGVYATVDRGGFGGAGAAAVMAAAAAFWAVNTGLIVP
                     AIVLDTGTGAADVWRDLAPTIPNYLAFGLLGTLLGYLYRQVGAVAVLVLASPIVIARS
                     VFLAFLRLRQAYARLELLYGFTGAVGPAADHDEDEMVATMLPELRRLLRTDLAQLHLD
                     AGKPLALLSASTGGASTGGASTGGGDRPGLGPASAAAAAIIAEVTRRGRAFRVAAGQD
                     DAFARALADGGLFDSLVAPVRVQGRVLGALLVANRQANEPPFTREDVQLFETVANHAS
                     VSLERGRLIDRLRHESTHDNLTGLANRTVLSAALEAALTRSELRGAGGAVLLMDLDRF
                     KEVNDTLGHDVGDALLREIGHRLAGLPVPGATIARLGGDEFGVLLPEVHGPADSARAA
                     RLLLDALRRPFPVDDIDLELGASIGIAQFPRDGQDAATLLRRADMAMYVAKADRLGWS
                     LYDRSADLSSHRRLAIASDLRRGIEAGELRVHYQPMADLRTGRLASMEALVRWQHPRF
                     GPLAPDEFIPLAEQTGLVRPLTHYVLARALSAVRRWRDLGFDLDVAVNLSVRVLLDPS
                     LPGRVEAALRHHGTPARWLTLEITETAMMSDPAGGDVLRRLAALGVTLSIDDFGTGYS
                     SLAYLRDMPVGEVKIDRSFVANCAENRADAVLVRSIVDLARHLGLRVVAEGVEDQATW
                     NQLEAAQCDVAQGYFLTEPLPEESVAAWCER"
     gene            complement(2671..>3270)
                     /locus_tag="VF954_03265"
     CDS             complement(2671..>3270)
                     /locus_tag="VF954_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530131.1"
                     /translation="LHRAEQAERAAALVAEREEHLRRGLRARSISALRPAGITEAIQA
                     SLAWAGVHTAADFVDVEVADRSAGGSSVLLADGRRARLESLSSGPGRALADWRRRLEA
                     RLIAERAHLSDECEAAILARFEPRRLALADAAAALRADTAREREHIRHAHAARRQALA
                     DDLVAEQRRLAATQHRVETDLADARRALAEAEWRARALR"
BASE COUNT          458 a         1153 c         1260 g          399 t
ORIGIN      
        1 accgttcgca ccaggccgcc acgctctcct cgggcagcgg ctcggtcagg aagtagcctt
       61 gagccacgtc gcactgggcg gcctccagct ggttccaggt ggcctggtcc tcgaccccct
      121 cggcgaccac ccgcagtcct aggtgacggg ccaggtccac gatcgaccgg acgagaacgg
      181 cgtcggcccg gttctcggcg cagttggcca cgaagctgcg gtcgatcttc acctccccca
      241 cgggcatgtc ccggaggtag gccagggagg agtaccccgt gccgaagtcg tcgatggaca
      301 gggtgacgcc gagggccgcc aggcggcgca ggacgtcgcc gccggcgggg tcggacatca
      361 tggccgtctc tgtgatctcg agggtgagcc agcgcgccgg agtgccgtgg tgccggagcg
      421 cggcctccac ccggccgggg agggaggggt cgaggagcac ccgcacggag aggttgaccg
      481 ccacgtcgag gtcgaagccg aggtcgcgcc agcgccgtac ggccgacagg gcgcgagcca
      541 gcacatagtg ggtgaggggc cgcaccaagc cggtctgctc ggcgaggggg atgaactcgt
      601 ccggcgcgag cgggccgaac cgggggtgct gccaccgcac gagcgcctcc atgctcgcca
      661 accgcccggt gcggagatcg gccatgggct ggtagtggac ccgcagctcg cccgcctcga
      721 tgccccgccg caggtccgag gcgatcgcca ggcgccggtg gctggacagg tcggcggagc
      781 gatcgtagag ggaccagccg aggcgatcgg ccttggcgac gtacatggcc atgtcggctc
      841 tccggagcag cgtggcggcg tcctggccgt ccctgggaaa ttgggcgatg ccgatgctcg
      901 ccccgagctc gaggtcgatg tcatccaccg ggaacggccg ccgcagggca tcgagcagga
      961 gccgagcggc ccgggcggag tccgccgggc cgtgcacctc gggtagcagc accccgaact
     1021 cgtcgccgcc caggcgggcg atggtggcac cgggcacggg aagcccggcc agccgatgcc
     1081 cgatctcgcg cagcagggcg tcgccgacgt cgtggccgag cgtgtcgttc acctctttga
     1141 accggtccag gtccatcagc agcaccgccc caccggcgcc ccggagctcg gagcgcgtca
     1201 gagcggcctc gagggcggcg ctgaggaccg tgcggttggc cagtcccgtc aggttgtcgt
     1261 gggtggactc gtggcggagg cggtcgatga gccggccccg ctcgagggag acgctcgcgt
     1321 ggttggccac cgtctcgaac agctgcacgt cctccctggt gaagggtggc tcgttagcct
     1381 gccggttggc gaccaggagg gccccgagga cccggccctg cacccggacc ggggcgacga
     1441 gcgagtcgaa cagccccccg tcggccagcg cccgggcgaa ggcgtcgtcc tgcccggcgg
     1501 ccacgcggaa ggcgcggccc cgccgggtga cctccgcgat gatggcggcg gcggcggcgc
     1561 tggccgggcc caacccgggc cggtccccgc cgccggtgga tgcgccgccg gtggatgcgc
     1621 cgccggtgga tgcgctcagc agggccagcg gtttcccggc gtcgaggtgc agctgggcca
     1681 ggtcggtgcg gagcaggcgc cggagctcgg gaagcatggt ggccaccatc tcgtcctcgt
     1741 cgtggtcggc ggccgggccg accgcgccgg tgaacccgta caacagctcc agccgggcat
     1801 aggcctggcg gagccggagg aaggccagga acaccgaccg ggcgatgacg atcggcgagg
     1861 ccagcaccag gacggccacc gctccgacct gccggtacag gtacccgagg agcgtgccga
     1921 gcaggccgaa cgccaggtag ttcgggatcg ttggcgccag gtcccgccac acgtcagccg
     1981 cgccggtccc ggtgtcgagg acgatggccg gcacgatcaa ccccgtgttg acggcccaga
     2041 atgcggctgc ggcggccatg acggcggccg caccggcccc gccgaagccg ccccggtcga
     2101 cggtggcgta aacgccttcg gcggccgccg cggcgagcag gtactgggcg cagttgaaca
     2161 ccacgatgcg gtaccgccgc cggcggaggt ggtcgatcgc gagtccgcca caggctccgg
     2221 tgagggccac gccgagcagg gccccgccgc cccggtaggc ggtcacggtc gccatcgcca
     2281 ccatgaagct ggggctgacg cgggtcgagg tcgggagccg aacggcgacg ttctcggccc
     2341 agacgagcag acccgcgaag agggccaacg caaccggatc ggggcctcgt gacgcggtca
     2401 gggcggcggc ggcgagcaca gcggcggctg tcaccgcggt gcccgcctcc aaccaaccca
     2461 gcctccggcg gtccgtgccg taacccacct tcgtttgctc cgttcccctg gtcccggccc
     2521 tgacccgaaa tcgtaggggg aggggcgcgc tgaaggggtg ataccaccca acgtggtcgt
     2581 ccggggcctt gccgaccagc gagagtggca ctgaacggtg gttagaagcc tgaacggtgg
     2641 ttggtgaccg gtggtgaccg cttggtgggt tcagcgaagg gcccgggcgc gccactctgc
     2701 ctcggcgagg gcccgccggg cgtcggccag gtcggtctcg acccggtgct gggtggcggc
     2761 cagccgccgc tgttcggcca cgaggtcgtc ggcgagggcc tggcgccggg cggcatgagc
     2821 gtgccggatg tgctcgcgct cccgggcggt gtcggcccgc agggcggcgg cggcgtcggc
     2881 cagggccagc cggcgcggct cgaaacgggc caggatggcc gcctcgcact cgtcggacag
     2941 gtgggcccgc tcggcgatga gccgggcctc cagacgtcgg cgccaatcgg cgagggcccg
     3001 cccgggcccg gagctgaggc tttcgaggcg agccctgcgg ccgtcggcga gcagcaccga
     3061 tgacccgcca gcgctccggt cggccacctc gacatcgacg aagtcggcgg ccgtatggac
     3121 ccccgcccag gccaggctgg cctggatggc ctcggtgatc cccgccggac gcagcgcgga
     3181 gatcgaacgg gcccgcagac cgcgccggag gtgctcctcg cgttcggcca cgagcgccgc
     3241 ggcccgctcc gcctgctccg cccggtgcag
//
LOCUS       DASWGG010000179         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_821448, whole genome shotgun sequence.
ACCESSION   DASWGG010000179 DASWGG010000000
VERSION     DASWGG010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821448"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..907
                     /locus_tag="VF954_03270"
     CDS             122..907
                     /locus_tag="VF954_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9530132.1"
                     /translation="MTTKAATLDMLRTPGLAVERPLELSGQWFNELCLWLQSPIGSLG
                     PLGWPMVLIPEIIDSRYRLGDSALQVYQRVEWSGPVPTGEFTASAHVEWVSGRGGSFE
                     AGLSTEARRGGQRLAHCLLVARMSGDLESYGERDLPPRPELDETTLSSRRTLVVDEAT
                     IRQFAELAGTRYPVHDDERYAWSQGYPTILVQGLLLFMTQLYQYGVGPSGRGEMWFRR
                     AVPAGSLLESCQSTADPMLWAMRLVGGGEVAAVARITSSRPSG"
     gene            937..>1509
                     /locus_tag="VF954_03275"
     CDS             937..>1509
                     /locus_tag="VF954_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530133.1"
                     /translation="MGTFISGDTPGPPGLVDWDYWLNHWPDHPIAVTRANAADGYPRP
                     LTPLSQDLVLTYEEAGVRRFYFDVLGVLTPADAPDPYMLAYYGLVYLNADQLAGLGEA
                     TPGSSRQAMYQQFFGLEADPAYRPPKPTAKARLAEAVTGLKVAPRTLRLARGVVAEID
                     RRKAAILAARPAGDLGSRSDDDLAAWLGRLD"
BASE COUNT          207 a          560 c          530 g          212 t
ORIGIN      
        1 gtcgtcgtcg aggccctccc gaagacctcc atcggcaaga tcgagaagaa ggcgctgcgg
       61 gagcgcctgg gccaggagcg tgcgctatga ttgaacagtc agtcagttca ggaggggcac
      121 ggtgaccacc aaggcagcca ccctcgacat gctgcggacg ccgggcctgg ccgtcgaacg
      181 gccgctcgag ctgtcgggcc agtggttcaa cgagctgtgc ctgtggctgc agtcgccgat
      241 cggctcgctg ggcccgctgg gctggccgat ggtgctcatc ccggagatca tcgactcccg
      301 ctaccgcctc ggcgacagcg ccctccaggt ctaccagcgg gtggagtggt ccgggccggt
      361 gccgacgggg gagttcaccg cctcggccca cgtggagtgg gtgtcgggcc gcggcggctc
      421 gttcgaggcc ggtctgtcca ccgaggcgcg ccgcggcggt caacggctcg cccactgcct
      481 gctcgtcgcc cggatgagcg gggacctgga gtcctacggg gagcgggacc tcccgccccg
      541 acccgagctc gacgagacca cgctgtccag ccggcgaacg ctggtggtcg acgaggccac
      601 catccgccag ttcgccgagt tggccggcac ccgctacccg gtccacgacg acgagcgcta
      661 cgcctggtcg cagggctacc cgacgatcct cgtccagggc ctgctgctgt tcatgacgca
      721 gctctaccag tacggcgtgg ggcccagcgg ccgcggcgag atgtggttcc ggcgggcagt
      781 cccggccggg agcctcctgg agtcgtgcca gagcaccgcc gacccgatgc tgtgggcgat
      841 gcgcctcgtc ggcgggggcg aggtcgccgc cgtcgcccgc atcacatcgt cacggccgtc
      901 gggctgaccg gcggccgccg agggggaggt catccgttgg gcacgttcat ttcaggggac
      961 acccccgggc ccccgggact cgtcgattgg gactactggc tcaaccactg gcccgaccac
     1021 ccgatcgccg tgacgcgggc caacgccgcc gacggctacc cccggcccct caccccgctc
     1081 agccaggacc tggtcctcac ctacgaggag gccggggtcc ggcgcttcta cttcgacgtc
     1141 ctgggggtgc tgacgccggc cgacgcccca gacccctaca tgctggccta ctacggcctc
     1201 gtctacctca acgccgacca gctggccggg ctgggggagg cgacgccggg cagctcccgg
     1261 caggccatgt accagcagtt cttcgggctg gaggccgacc cggcctaccg cccgccgaaa
     1321 ccgacggcca aggcccggct ggccgaagcg gtcaccgggc tcaaggtggc gcctcgcacg
     1381 ctgcgcctgg cccgcggcgt cgtcgccgag atcgaccgcc gaaaggcggc gatcctggcc
     1441 gcccggccgg ccggcgatct cggttcccgg tccgacgacg atctcgccgc ctggctcggc
     1501 cgtctcgac
//
LOCUS       DASWGG010000180         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1464476, whole genome shotgun sequence.
ACCESSION   DASWGG010000180 DASWGG010000000
VERSION     DASWGG010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464476"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VF954_03280"
     CDS             <1..747
                     /locus_tag="VF954_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX9530134.1"
                     /translation="GGTGGDPPALGAKLLVRPGGSLPGGSLGNADLDRVVARDVRAAL
                     EAGLSATRHYGRRGQARQRQVSVFIESFAPPPRMIIFGAVDFTAALARVAKVLGYRVT
                     VCDARPVFATAARFPMADEVVVDWPNRHLTEVGPALGPRDAVCVLTHDPKFDVPAIVS
                     ALATRVGYLGAMGSRRTHHERAARLREAGVTDAQLARVLAPIGLDLGARTPEETAVSI
                     CAEIIALRTGRVAPSLRDTEGPIHRDPAPA"
     gene            744..1304
                     /locus_tag="VF954_03285"
     CDS             744..1304
                     /locus_tag="VF954_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX9530135.1"
                     /translation="MSRAAVVLAAGGGSRFAGPAHKLLAPFRGRPLVTWAVAAAAEAA
                     LDETIVVSGAIDLVGVVPEGVTIIDNPGWAQGISGSLRGAVAWAERQGHEAIVVGLGD
                     QPLVPAEAWRTVAEAHGAAILVATYAGQRRNPVRLDRSVWAMLPVSGDEGARALVRRR
                     PELVAEVACEGDPIDVDTVEDLRRWS"
     gene            1295..>1708
                     /locus_tag="VF954_03290"
     CDS             1295..>1708
                     /locus_tag="VF954_03290"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9530136.1"
                     /translation="MELSNDFRVRVPIEQAWSVLTDVERIAPCMPGAQLEEVAGDEYR
                     GSVKIKVGPITAQYKGKATFLELDAAGHRAVLRAEGRDTRGQGNANATITATLEPHAL
                     GTSVTVLTDLAVTGRVAQFGRGVLADVSANLLRQFV"
BASE COUNT          221 a          614 c          650 g          223 t
ORIGIN      
        1 ggggggacgg gcggggaccc tccggcgctc ggggccaagc tcctggtgcg cccgggcggg
       61 tccctgccgg gcgggtccct gggcaatgcc gacctcgacc gggtggtcgc ccgggacgtc
      121 cgcgccgccc tcgaggccgg gctgtcggcc accaggcact acggccggcg gggccaggcc
      181 cgccagcgcc aggtgagcgt gttcatcgag tcgttcgccc caccgccccg catgatcatc
      241 ttcggcgccg tcgacttcac ggcggccctg gcccgggtgg ccaaggtgct cggctaccgg
      301 gtgacggtgt gcgacgcccg gccggtgttc gccaccgcgg cccggttccc gatggccgac
      361 gaggtcgtcg tcgactggcc caatcgccac ctgaccgagg tgggcccggc gctcgggccg
      421 cgggacgcgg tgtgcgtgct cacccacgat cccaagttcg acgtgccggc catcgtctcg
      481 gccctggcca ccagggtcgg ctatctcggg gcgatgggat cgcggcgcac ccaccacgag
      541 cgcgccgcgc gcctgcgtga agccggcgtc acggacgcgc agctggcccg ggtgctcgcc
      601 cccatagggc tcgacctcgg ggcccgcacc cccgaggaga cggcggtgtc gatctgcgcc
      661 gagatcatcg ccctccgcac cgggcgggtg gcgccctcgc tgcgcgacac cgagggcccg
      721 atccaccggg acccggcgcc ggcgtgagcc gcgccgcagt cgtgctggcc gcgggcggag
      781 gctccaggtt cgccggaccg gcgcacaagc tgctcgcgcc gttccggggc cgcccgctcg
      841 tgacctgggc agtggcggcg gcggccgagg ccgcgctgga cgagacgatc gtcgtgtcgg
      901 gcgccatcga tctggtggga gtggttcctg agggcgtgac catcatcgac aacccgggct
      961 gggcccaggg catctccgga tcgctccgtg gcgcggtggc gtgggcggag cgccagggcc
     1021 acgaggcgat cgtggtgggc ctgggcgatc agcccctcgt gccagccgaa gcctggcgca
     1081 ccgtggccga ggcgcatggc gcggccatcc tggtggccac ctacgccggc cagaggagga
     1141 acccggtgcg gctcgatcgg tccgtgtggg ccatgctgcc cgtctcgggt gacgaagggg
     1201 cccgcgcctt ggtcaggcgc cggccggagc tcgtggcgga agtagcgtgc gaaggcgacc
     1261 cgatagacgt ggacaccgtg gaggacctca ggcgatggag ctaagcaacg acttccgagt
     1321 gcgcgtgccg atcgagcagg catggagcgt gctgaccgat gtcgagcgca tcgcgccgtg
     1381 catgcccggc gcccagctcg aagaggtggc gggtgacgag taccggggga gcgtcaagat
     1441 caaggtgggg cccatcaccg cccagtacaa gggcaaggcc acgttcctcg agctggatgc
     1501 cgccggccat cgggcggttc tccgggccga agggcgcgat acccggggtc agggcaacgc
     1561 caacgccacg atcaccgcca ccctcgagcc ccacgcgctc ggcaccagcg tgaccgtgct
     1621 caccgacctc gccgtcaccg gccgggtggc gcagttcggc cgcggcgtgc tggccgacgt
     1681 gagcgccaac ctcctgcgcc agttcgtg
//
LOCUS       DASWGG010000181         3140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2020161, whole genome shotgun sequence.
ACCESSION   DASWGG010000181 DASWGG010000000
VERSION     DASWGG010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2020161"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VF954_03295"
     CDS             complement(<1..445)
                     /locus_tag="VF954_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530137.1"
                     /translation="MVGDRQRPARIRAEQERVLCSVVRHELYAYSPHHRARLDAAGLA
                     EGRVGSGTELARVEPIRLADIEDPASLVLRPAGASIRRYGGRGLAARLALARLTRRSG
                     ALNRVVIEPLYKPIQWMIEEGVPIGSSALDLEQLAELGRQVLELAG"
     gene            679..1665
                     /locus_tag="VF954_03300"
     CDS             679..1665
                     /locus_tag="VF954_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEX9530138.1"
                     /translation="MSQLRLDPLTGRWVVVSIDRATRPQAFTVRNLAVESSSRPCPFC
                     PGHEESTPPALESYGPSGSWLVRVVPNLFPAFDGEAPVVVSHLGPVFTQAPASGIHEV
                     LVLSPEHDADWGDISDAQAGLIMAAIRDRIEEHASVPSLRYSQAIVNAGREAGASIEH
                     PHGQLLGMPFVPRELVDEQAGFARFEGNCLLCTTIDAEESAGHRVVHSEERVLVICPF
                     WSGLPFEMLVLPRAHDPHLHRARPPDLAAVGRAIRGAIATLRHRLGDVAYNVVFHSAP
                     YRATGAFHWHVHLWPKLTSLAGFELGTGVLVNVVSPELAAEELRSAAVPAAN"
     gene            complement(1767..>3140)
                     /locus_tag="VF954_03305"
     CDS             complement(1767..>3140)
                     /locus_tag="VF954_03305"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEX9530139.1"
                     /translation="NQAWRAPLRAALDGLRDRATEVFERRGGAVLRDPWAARDAYIEV
                     MLGSVGREAFADAWVLGDPVVAFSLLESQRHAMAMYTSCGWFFHDLAGIETVQVMRYA
                     ARCMDLLVDIGEDPDQDGFLTALAAAQSNDPAEGDGRSVWERHVVPFRVDEARAAAEL
                     ALEDLLEARPAPDRVAAWELEVLDRGRLVRGGLVLAHGRVALTHRRTGRRTERVYAGV
                     NLGGFEVFGASRPADPSVGRDEALVHGLRRSFEAGMRVTALLRTVVDDFGPDEFGLDS
                     ALPDAAERIMARAAGQLAARFAAAYDRLYSDHRDTLESLAAAGYPLPPVLRAPAELAL
                     ARRIEAEVVAQAGSLDPADYAAAVGVARQARAGGFVLDTPQARATVERLLLLATERAT
                     GGPDARARAEGVAAARALLDVADELELHPDVDRAQEVLFAALDIAPERAEALRPLAER
                     LWLAPGP"
BASE COUNT          438 a         1202 c         1099 g          401 t
ORIGIN      
        1 ggccggcgag ctcgagcacc tgccggccga gctcggccag ctgctccagg tcgagggccg
       61 agctcccgat gggcacgccc tcctcgatca tccactggat cggcttgtag agcggttcga
      121 tgaccacgcg gttgagcgcg cccgaccgcc gcgtgaggcg ggccagggcc agtcgggcgg
      181 ccagcccccg gccgccgtac cggcggatgg aggccccggc ggggcgcagc accaggctgg
      241 ccggatcctc gatgtcggcg agccggatgg gctcgacccg ggccagctcg gtcccggagc
      301 caacccgtcc ctccgccagc ccggccgcgt cgagccgggc gcggtgatgg ggcgagtagg
      361 cgtagagctc gtggcggacg acggagcaga gcacacgctc ttgctccgcc cggatgcgcg
      421 cggggcgctg tcggtcgccg accactgtcg caccttaggg ccaagccacg gactactggg
      481 gtgacgcttg aacacccagc cgccgagaac ggggcaaaac ggccttcgtt cgcccaagct
      541 gcgcccaagc agcgcccgag tagctctgcg ctcggccctc atcggccgtg gagctcgggc
      601 caacgccaca ttggaccgtg agggcactac cttgagagcc gttgacatgg cctccgatcc
      661 acactcccga ctagcccagt gagccagctc cgcctcgacc ctttgaccgg ccgctgggtc
      721 gtggtgagca tcgaccgggc gacccgaccg caggccttca cggtgcgaaa tctcgccgtg
      781 gagtcgtcct cccgcccgtg cccgttctgc cccggccatg aggagtccac cccgccggcc
      841 ctcgagagct acggcccgtc gggttcgtgg ctggtccgcg tcgtccccaa cctgttcccc
      901 gcattcgacg gcgaggcacc ggtcgtggtc tcccatctcg gacccgtctt cacccaggcg
      961 ccggccagcg gcatccacga ggttctcgtc ctgtcgcccg agcacgacgc cgactggggc
     1021 gacatctccg acgcgcaggc cggcctgatc atggccgcca tccgggaccg catcgaggag
     1081 catgcctcgg ttccctcgct caggtacagc caggccatcg tcaacgccgg ccgcgaggca
     1141 ggagcctcca tcgagcaccc gcacgggcag ctgctgggga tgcccttcgt gccacgcgag
     1201 ctggtcgacg agcaggcggg gttcgcccgc ttcgagggga actgcctgct gtgcacgacc
     1261 atcgacgccg aggagtcggc gggccaccgc gtggtgcaca gtgaggagcg ggtgctggtg
     1321 atctgcccct tctggagcgg cctgcccttc gagatgctcg tcctgccgcg ggcccatgac
     1381 ccgcacctgc acagggcccg cccgcccgat ctggccgcgg tgggccgggc catccggggc
     1441 gccatcgcca ccctcaggca ccgtttgggc gacgtggcct acaacgtcgt gttccattcc
     1501 gccccgtacc gggccactgg cgccttccac tggcacgtcc acctctggcc caagctcacc
     1561 agcctggccg gcttcgagct cgggacgggc gtgctcgtca acgtggtgtc gcccgagctg
     1621 gccgccgagg agctgcgcag cgccgccgtc ccggccgcca actaggccaa ccgcgcaggc
     1681 cgtttcccgg ctgcgaaccc attcttaaag caggaagtgc actggagcga cattgaagcc
     1741 ttgaagaatg ccgaaaaccg gctcccctag ggtcccggcg ccagccagag ccgctcggcc
     1801 agcggacgca gggcctcggc ccgctcgggc gcgatgtcca gcgccgcgaa gagcacctcc
     1861 tgggcccggt ccacgtcggg atgcagctcg agctcgtcgg ccacgtcgag cagcgcccgg
     1921 gcggccgcca caccctcggc ccgcgcccgg gcatcgggcc cacccgtagc ccgctcggtg
     1981 gcgagcagga ggagccgctc gaccgtcgcc cgggcctggg gggtgtcgag gacgaacccg
     2041 ccggcccggg cctggcgggc gacaccgacc gcggcggcgt agtcggccgg gtcgagggac
     2101 ccggcctggg cgaccacctc ggcctcgatc ctccgggcca gggccagctc cgcgggcgcc
     2161 cgcaggactg ggggcagcgg gtagccggcg gccgccagcg actcgagggt gtctcgatgg
     2221 tccgagtaca gccggtcata ggccgccgcg aaccgggccg ccagctggcc ggccgcccgc
     2281 gccatgatcc gctcggccgc gtccggcagc gcggagtcca gcccgaactc gtcaggcccg
     2341 aagtcgtcca cgaccgtgcg cagcagggcc gtcacccgca tgccggcctc gaacgagcgg
     2401 cgcagcccgt gcacgagcgc ctcgtcgcgc cccacggacg ggtcggcggg gcgggacgcg
     2461 ccgaagacct cgaacccgcc caggttcacg cccgcgtaga cgcgctcggt ccggcgaccg
     2521 gtccggcggt gggtgagcgc cacccggccg tgggccagca ccaggccgcc ccgcacgagg
     2581 cggccccggt cgagcacctc cagctcccac gccgccaccc ggtcgggggc gggccgggcc
     2641 tcgagcaggt cctcgagggc gagctcggcg gcggctcgag cctcgtcgac gcggaacggc
     2701 acgacgtggc gctcccagac cgaccggccg tcgccctcgg ccgggtcgtt gctctgggcg
     2761 gcggccaagg cggtgaggaa cccgtcctgg tcggggtcct cgccgatgtc gaccagcagg
     2821 tccatgcacc gcgccgcgta ccgcatgacc tggacggtct cgatcccggc caggtcatgg
     2881 aagaaccagc cgcagctcgt gtacatggcc atggcgtggc gttgtgactc caggagggag
     2941 aaagcgacca ccggatctcc gaggacccac gcgtcggcga acgcctcgcg cccgaccgag
     3001 ccgagcatca cctcgatgta ggcgtctcgt gccgcccagg ggtctcgcag cacggcgccg
     3061 ccgcggcgct cgaacacctc cgtggcccga tcgcgcagcc cgtcgagcgc ggcccgcaac
     3121 ggcgcccgcc acgcctggtt
//
LOCUS       DASWGG010000182         5113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2342333, whole genome shotgun sequence.
ACCESSION   DASWGG010000182 DASWGG010000000
VERSION     DASWGG010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2342333"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VF954_03310"
     CDS             complement(<1..400)
                     /locus_tag="VF954_03310"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HEX9530140.1"
                     /translation="MHFLIEQDLDGALEDVETAFFDPAFLAGLAAVPNLAAPQLLSQE
                     RTDGVIHQRVRYRFTGELNRAVSRAVDRDRLTWIEDSTFRRPLHRTDWTVLPDHYADR
                     LSCSGSFELSPAGSRTHRVADGELTVHVPLV"
     gene            complement(425..2548)
                     /locus_tag="VF954_03315"
     CDS             complement(425..2548)
                     /locus_tag="VF954_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HEX9530141.1"
                     /translation="MAERSTPLLGGLTAAQREAVTIEAAPLCLLAGAGSGKTRVLTRR
                     IAWRVSTGAADGAHVIALTFSRKAAGELRARLAELGVRDQVAAGTFHGLAYAQLRRRW
                     ADHGVPAPTLLDRKAGLLSRLLRDRGERGGGGGGAAAGDVAAEIEWAKARLIPPEGYT
                     RAARLARRRVGLALEEVAELYERYEATKRRRGLVDFDDLLVRCAEALERDSEFAAGQR
                     WRFRHFFVDEFQDANPAQFRLLQAWVGGRPDLCVVGDPNQAIYSWNGADPALLTEFAE
                     RFPGTTVVRLDDNFRSSPQILAVANAVLGQARSGWAPDDDRPPAGRAHLPDGAVPTVR
                     SFATDAAEAAGVAQAVRRVRRPNTIWSRIAVLARTNAQLRVLSSAFRAAAIPCRVTGD
                     RDLLARPDVRAVLDQLPRAPAGMAFLDRFADLVAGGAATPDTAAVGPARGVAAGMAAG
                     IGADSAGTTVLRLAREFADLDSAPTLDGFLAWLQVTSGSDSPATGDAVELTTFHRAKG
                     LEWPVVYLVGLEQGLVPIGHATTAAAEAEERRLLYVAMTRAQSELHCSWAERRSFSGR
                     MVEREPSPWLDVIEVACRTLEHGTVADGATVRRLLADQRDRLGRPRVGSAAARGSRVR
                     PAAAIEVRADPAVLADLKAWRAGQARASGVPAYVIFHDATLAAVAEARPRSAEELLGL
                     PGLGPVKAARYGDQLLALVARHGAA"
     gene            2661..3407
                     /locus_tag="VF954_03320"
     CDS             2661..3407
                     /locus_tag="VF954_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9530142.1"
                     /translation="MLRSFADGQLFGQTHGSAPFRVLALHGWRRSSADWGQVLAGLDG
                     VALDLPGFGATPAPPEPWGSRRYAEAVAPVLDEMAPGPVVIGHSFGGRVALWLAADHP
                     DQVGGLVLTGVPFGRSKEERGPRPSAGFRLGRALHRAGVLSDARMERLREKHGSADYV
                     HATGVMRQVLVAAVGETDRSAYDDALRTITCSVELVWGDDDTAAPLAVAKRAAALVSD
                     ARLTIEPGAGHLTPLSAPRALADAVRRQLA"
     gene            3404..4945
                     /gene="murF"
                     /locus_tag="VF954_03325"
     CDS             3404..4945
                     /gene="murF"
                     /locus_tag="VF954_03325"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX9530143.1"
                     /translation="MTSVAPRDTVVMVAALAASGVAAVRWLRVAQAEHYAPGSVSRFA
                     ARWWWVWQPPNRVLLAAALAGLLGMAVTPAAGMVTTATVATGPFGLTLRGRTSKLVWT
                     RRLRTLAGAWAFIEVLIVAVGLSLGAGPVVAVLAAVAAPVLVDAALVVTAPLERRLAQ
                     PFVDAAAARLRSIGPTVVAVTGSYGKTTTKVYIAHLLAGTKTVLATPKSYNNRNGLAR
                     TINERLAPGTEVLVAEMGTYGPGEIAEMCELVPPDVSAITAIGPVHLERMGSEERITA
                     AKAEILHHARAVVLNVDEPRLAALADRAGADGRRVVRCSAENVAADVLVSPQGPLTLV
                     VANGEEVARLPRLDAPPTNVAIAVAVAFLIGVPADEIATRLADLPVVDNRLSVGNTSS
                     GATVIDDTFNSNPAGAAAALEKLRGLAAPEGQRVVITPGMVELGPRQAPENEVLARAA
                     SSVATTLIVVGRTNRPALVAGGRDGPAAVLCVETRDEAVAWARDHLGAGDVVLYENDL
                     PDIYA"
BASE COUNT          657 a         1875 c         1928 g          653 t
ORIGIN      
        1 cgacgagtgg cacgtggacg gtgagctcac cgtcggcgac ccgatgggtt cggctcccgg
       61 ccgggctcag ctcgaaggac ccggagcacg acaggcgatc cgcgtagtgg tcgggcaaca
      121 ccgtccaatc cgtgcggtgg agagggcggc ggaaggtgct gtcctcgatc catgtcagcc
      181 tgtcccggtc gacggcccgg gagacggcgc ggttcagctc ccccgtgaag cggtagcgga
      241 cacgttgatg gatgactcca tccgtgcgct cctgggagag cagctgcggc gccgccaggt
      301 tgggcacggc cgccaggccg gccaggaagg cgggatcgaa gaacgccgtc tccacgtcct
      361 cgagggcccc gtcgaggtcc tgttcgatca ggaaatgcac cggccgtcag cctatggtgc
      421 cgcatcaagc cgccccgtgc cgggccacca gggcaagtag ctggtctccg taccgggccg
      481 ccttgacggg gcccagccca gggagtccga ggagctcctc ggcggatcgc gggcgggcct
      541 cggcgactgc ggcgagggtg gcgtcgtgga agatcacgta ggcgggcacg cccgatgccc
      601 tggcctggcc ggctcgccac gccttgaggt cggcgagcac ggccggatcg gcccggacct
      661 cgattgccgc ggccggacgc acacgcgagc cgcgggccgc ggccgacccg acccggggcc
      721 gaccgaggcg gtcgcgctgg tcggcgagca ggcgccgcac ggtggcgccg tcggccaccg
      781 tgccgtgctc cagggtccgg caggcgacct cgatgacgtc gagccagggc gatggctccc
      841 tctcgaccat ccgcccggag aacgaccgcc gctcggccca cgagcagtgc agctcggact
      901 gggcccgggt catggccacg tacagcagcc tgcgctcctc ggcctcggcg gccgccgtgg
      961 tggcatggcc gatcgggacc agaccctgct ccagcccgac caggtagacg accggccact
     1021 ccaatccttt ggcgcggtgg aacgtggtca gctcgacggc gtctcccgta gcagggctgt
     1081 cgctccccga tgtgacctgc aaccaggcga ggaagccgtc gagggtgggt gcggagtcga
     1141 ggtcggcgaa ctcccgcgcc aggcgcagga ctgtcgtccc ggcgctgtcg gccccgatgc
     1201 ccgccgccat gcccgccgcc acgcctcgcg cggggcctac cgcggccgtg tcgggtgttg
     1261 cggccccccc ggcgaccagg tcggcaaacc ggtcgaggaa cgccatgccg gccggagccc
     1321 ggggcagctg gtcgagcacg gctcgtacgt cagggcgagc cagcaggtcc cggtctccgg
     1381 tcacccggca cgggatggcg gcggcccgga aggcggagct cagcacgcgc agctgggcgt
     1441 tggtccgggc gagcacggcg atccgggacc aaatggtgtt gggccgccgg acgcgccgga
     1501 ctgcttgcgc cacccccgcc gcttcggccg cgtcggtggc gaaggaccgc accgtgggca
     1561 cggcaccgtc cgggaggtgg gcccggccgg cgggcgggcg gtcgtcgtcc ggagcccagc
     1621 cggagcgcgc ttgaccgagc acggcgttgg cgacggccaa gatctggggt gaggatcgga
     1681 agttgtcgtc gagccggacc acggtggtgc cggggaagcg ctcggcgaac tcggtgagga
     1741 gggcggggtc cgcgccgttc caggagtaga tcgcctgatt ggggtcaccg accacgcaca
     1801 ggtcgggtcg gcctcccacc caggcctgga gcaggcggaa ctgggccggg ttggcgtctt
     1861 ggaactcgtc cacgaagaag tgccggaacc gccaacgttg gcccgcggcg aactccgagt
     1921 cgcgctccaa ggcctccgcg caccgcacca ggaggtcgtc gaagtccacc aggccgcgcc
     1981 gccgcttggt cgcctcgtag cgctcgtaga gctcggccac ctcctccagg gccaggccga
     2041 cccgccgcct ggcgagccgg gcggcgcggg tgtagccctc gggagggatg agtcgggcct
     2101 tggcccattc gatctcggcg gccacgtcgc cggcggcagc gccgccccca ccgcccccgc
     2161 gctcaccccg gtcccggagc aggcgcgaga gcagccccgc cttccggtcc aggagggtcg
     2221 gggccggcac cccgtggtcg gcccagcgcc gccggagctg ggcataggcc agtccgtgga
     2281 acgtcccggc cgcgacctgg tcgcgcacgc cgagctcggc gagccgggcc cgcagctcgc
     2341 ccgcggcctt ccggctgaag gtaagggcga tgacgtgcgc cccgtcggcg gcaccggtgg
     2401 acacgcgcca ggcgatgcgc cgggtgagga cccgtgtctt tcccgagccc gcgcccgcca
     2461 gcaggcaaag gggagcggcc tcgatcgtga ccgcttcgcg ctgcgcggcc gtcagcccgc
     2521 cgagcagcgg agtggagcgt tcagccatcg ttggtcgacc ataacgaggg cctgtgacag
     2581 cccggtggat ggtcggcggg ggtggcccgg gtatcgtgcc ctcgccatgt tgaagtgctc
     2641 gccatgctga agtgcccgcc atgctgaggt cgttcgccga cgggcagctc ttcggccaga
     2701 cgcacggctc cgccccgttc cgcgtgctgg ccctgcacgg ctggcgacgc agctccgccg
     2761 actggggcca ggtgctcgcc ggcctcgacg gcgtggcgtt ggacctgccg ggcttcggcg
     2821 ccacgccggc gccgcctgag ccgtggggct cgcgccgcta cgccgaggcc gtggcccccg
     2881 tgctcgacga gatggcgccg gggcccgtgg tgatcggcca ttccttcggc ggccgggtcg
     2941 cgctgtggct ggccgccgac caccccgacc aggtaggcgg cctggtgctg acgggcgtgc
     3001 cgttcgggcg cagcaaggaa gagagggggc cccggccgag cgccggcttc cgcctcggcc
     3061 gggccctgca ccgcgccggc gtgctgtccg acgcccgcat ggagcggctg cgcgagaagc
     3121 acggctcggc cgactacgtc cacgccaccg gtgtgatgcg ccaggtcctc gtggccgcgg
     3181 tgggggagac cgaccgctcg gcctacgacg acgccctgcg gaccatcacg tgctccgtcg
     3241 agctggtgtg gggcgacgac gacaccgccg cgcccctcgc ggtggcgaag cgggcggccg
     3301 ccctggtgag cgacgcccgc ctgaccatcg agcccggcgc cggccacctg acgccactca
     3361 gcgcgccgcg cgccctggcc gatgccgtcc ggcggcagct ggcgtgacct cggtcgcgcc
     3421 tcgcgacacc gtcgtcatgg tggccgccct cgcagcatcc ggggtggccg cggtccgctg
     3481 gctccgggtg gcgcaggccg agcactacgc gcccggctcg gtctcgcgct tcgcggcccg
     3541 gtggtggtgg gtgtggcagc cccccaaccg ggtcctgctg gcggcggcgc tggccgggtt
     3601 gctgggtatg gcggtcacac ccgcggcggg catggtgacc acggccacgg tcgcgacggg
     3661 acccttcggc ctgacgctgc ggggaaggac gtcgaagctc gtctggaccc gccggctgcg
     3721 caccctggcc ggcgcgtggg ccttcatcga ggtgctgatc gtggccgtcg gcctgagcct
     3781 gggcgccggg ccggtcgtgg ccgtcctcgc cgcggtggcc gcgcccgttc tggtggacgc
     3841 cgccctggtc gtcaccgcgc cgctcgagcg ccggctggcg cagccgttcg tcgacgccgc
     3901 cgcggccagg ctgcggtcga tcggcccgac ggtggtggcc gtgaccggct cctacgggaa
     3961 gacgaccacc aaggtctaca tagcccatct cctggccggc accaagaccg tgctcgccac
     4021 gccgaagagc tacaacaacc gcaacgggct ggcccggacc atcaacgagc ggctggcgcc
     4081 ggggacggag gtgctcgtgg ccgagatggg cacctacggg cccggcgaga tcgccgagat
     4141 gtgcgagctg gtgccgccgg acgtcagcgc catcacggcc atcgggccgg tgcacctcga
     4201 gcgcatgggc agcgaggagc gcatcaccgc ggccaaggcc gagatcctgc accacgcccg
     4261 ggcggtggtc ctgaacgtgg acgagccccg cctggccgcg ctcgcggatc gggccggcgc
     4321 cgatggcagg cgggtggtcc gctgctcggc ggagaacgtg gcggccgacg tgctggtctc
     4381 gccccagggg ccgctcaccc tggtcgtggc caacggcgag gaggtggccc ggctcccccg
     4441 cctcgacgcc cccccgacca acgtcgccat cgccgtggcg gtggccttcc tgatcggcgt
     4501 gcccgccgac gagatcgcca cccgcctggc cgacctcccc gtggtcgaca accgcctgtc
     4561 cgtcggcaac acctcgtcgg gagcgacggt gatcgacgac acgttcaact ccaacccggc
     4621 gggcgcggcc gcggccctgg agaagctgcg gggcctcgcc gccccggagg ggcagcgggt
     4681 cgtcatcacc ccgggcatgg tcgagctcgg cccgcgccag gccccggaga acgaggtcct
     4741 ggcgcgagcc gcttcgtcgg tcgcgaccac gctcatcgtg gtcggccgga ccaaccggcc
     4801 cgccctcgtc gcgggcgggc gcgacgggcc ggctgcggtg ctgtgcgtcg agacccggga
     4861 cgaggcggtg gcctgggccc gggatcacct cggtgccggc gacgtcgtgc tatacgagaa
     4921 cgacctgccc gacatctacg cctgaccaca ggagcagcca tggcccagcc ggccgtcatc
     4981 ttcggcggga tctcccccga gcacgacatc agcatcctga cgggcctggc cgcggcccgg
     5041 gccctggcca cgagcggccg gccggtccac ggcctgtact gggacaagac gggcgagtgg
     5101 cacctcgtcg acg
//
LOCUS       DASWGG010000183         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1154637, whole genome shotgun sequence.
ACCESSION   DASWGG010000183 DASWGG010000000
VERSION     DASWGG010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154637"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VF954_03330"
     CDS             <1..243
                     /locus_tag="VF954_03330"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441992.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX9530144.1"
                     /translation="HAFLASSGRATYVAASDAEVLEALSLLAETEGIIPALEPAHAVA
                     WVARAAGKEIPAGSTVLVTLSGRGDKDAAQVMELLA"
     gene            240..1082
                     /gene="trpA"
                     /locus_tag="VF954_03335"
     CDS             240..1082
                     /gene="trpA"
                     /locus_tag="VF954_03335"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX9530145.1"
                     /translation="MSVSSATGGLLAGRVPVPDPPAGIGPLEAALRAAREEGRKLLVP
                     YLTGGLGPDWPRVLEAVAAAGADAVEVGIPFSDPVMDGPVIQEASTQALAAGATPPGV
                     IATLGGVVVGVPLAVMTYYNLVFHLGHRRFARSLADAGVAAAIVPDLPLDELGEWARA
                     ADDGGVETVLLAAPTTPDDRLARICDRCRGFVYGVGLMGVTGERAALASSASLMARRL
                     KAVTDKPVLIGIGVSSAAQAAEVSEDADGVVVGSALVRRLLEGAGPDGAAAFVAELRR
                     GLDG"
     gene            complement(1121..>2596)
                     /locus_tag="VF954_03340"
     CDS             complement(1121..>2596)
                     /locus_tag="VF954_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX9530146.1"
                     /translation="EVPALTRRRRVPPGGRVLTVGARLGLALAGWYLIERHTGDQSTS
                     RSGLSRRLRRAFERLGPTYIKLGQIISSGEGIFPEELVGQFRLLRDRVPAERFELVRA
                     IVEADLGRPLEAVFRSFERAPLAAASIAQVHAAVLSSGEPVVVKVQRPQVQSLVRRDL
                     AAMSWIAPALVGRIPVAALANPPALVELFAETIVEELDFRLEAENMLDVARILAETEQ
                     RSLVVPRPHPSLVTKRVLVMERLDGFAWEDVAGMHAAGIDTSAVVRAGMVAFLEGAML
                     YGVFHGDLHGGNLFVRPDGRVALLDYGITGRLDEVRRLAFLRLLMAGTVNDARSQIEA
                     LRDLGALPMDTDISEVMRELGVDQPIKDPTAMTPEELTREIQEIVKKLLAYGARLPKE
                     LMLFVKDMLFLDGALATLAPDVDLFAEITEIATYFATRHGDRIARDIGVDPRRTVIDL
                     EGFKASMGLTSDVERITYRDLQERRETIRRRMEDHRRARRR"
BASE COUNT          324 a          919 c         1002 g          351 t
ORIGIN      
        1 cacgccttcc tggccagctc cgggcgggcg acgtacgtgg ccgcctccga cgcggaggtg
       61 ctcgaggcgc tgtcgctgct ggccgagacc gagggcatca tcccggccct cgagcccgcc
      121 cacgccgtgg cgtgggtggc gcgggcggca ggcaaggaga tccccgcggg ctcgacggtg
      181 ctcgtcaccc tttcgggccg gggcgacaag gacgcggctc aggtcatgga gctgctggcg
      241 tgagcgtgtc gtccgccacg ggggggctcc tggccggccg ggtgcccgtc ccggaccccc
      301 ctgcggggat cgggcccctg gaggccgccc tgcgggccgc tcgggaggag ggccgcaagc
      361 tgctggtgcc gtacctcacc ggtggtctcg gcccggactg gccccgggtc ctcgaggcgg
      421 tggccgcggc cggggccgat gccgtcgagg tggggatccc gttctcggac cccgtcatgg
      481 acgggccggt catccaggag gcctcgacgc aggccctggc ggccggggcc accccgccgg
      541 gtgtcatcgc caccctgggc ggcgtcgtgg tcggcgtccc gctggcggtg atgacgtact
      601 acaacctggt cttccacctg ggccaccggc gcttcgcccg gtcgttggcg gacgccggcg
      661 tggcggccgc catcgtgccc gacctcccgc tggacgagct cggcgagtgg gcccgggccg
      721 ccgacgacgg gggcgtggag accgtgctgc tggccgcgcc caccacgccc gacgaccggc
      781 tggcgcggat ctgcgaccgg tgccgcggct tcgtgtacgg ggtggggctc atgggtgtga
      841 ccggggagcg cgccgcgctg gcgagcagcg cctcgctgat ggcccggcgg ctcaaggcgg
      901 tcaccgacaa gccggtgctc atcggcatcg gcgtctccag cgcggcccag gccgcggagg
      961 tgagcgagga tgcagatggc gtcgtggtcg gctccgccct ggtccggcga ctgctggagg
     1021 gagcggggcc ggatggtgcc gccgccttcg tggccgagct gcgccggggc ctggacgggt
     1081 aggggccagg ccggccccgg ggtgccaggc gggggcgcgg tcacctcctc cgggcgcgcc
     1141 ggtggtcctc catgcgccgg cggatggtct cccggcgctc ctgcaggtcg cggtaggtga
     1201 tcctctccac gtcgctggtg aggcccatgg aggccttgaa gccctccagg tcgatcaccg
     1261 tgcgccgtgg gtcgacgccg atgtcgcggg cgatccggtc gccgtgccgg gtggcgaagt
     1321 aggtcgcgat ctccgtgatc tcggcgaaca ggtcgacgtc gggcgccagg gtggccaggg
     1381 ccccgtccag gaacagcatg tcctttacga agagcatcag ctccttcggc aggcgcgccc
     1441 cgtaggcgag gagcttcttg acgatctcct ggatctccct cgtgagctcc tcgggcgtca
     1501 tggccgtcgg gtccttgatc ggctggtcca cgcccagctc ccgcatcacc tcgctgatgt
     1561 cggtgtccat gggcagggcg ccgaggtcgc gcagcgcctc gatctgcgag cgggcgtcgt
     1621 tgacggtgcc cgccatgagc aggcgcagga aggcgaggcg cctgacctcg tcgagccgcc
     1681 cggtgatgcc gtagtccagg agcgcgaccc ggccgtcggg ccggacgaac aggttcccgc
     1741 cgtgcaggtc accgtggaag acgccataga gcatggcgcc ctccaggaag gcgaccatgc
     1801 cggcgcgcac gacggcggac gtgtcgatgc cggcggcgtg catgccggcc acgtcctccc
     1861 acgcgaagcc gtccaggcgc tccatgacca gcacgcgctt ggtgaccagc gagggatgcg
     1921 ggcgggggac gaccagggac cgctgctccg tctcggccag gatgcgggcg acgtccagca
     1981 tgttctcggc ctcgagccgg aagtcgagct cctccacgat cgtctcggcg aagagctcga
     2041 cgagggccgg cgggttggcc agggcggcca ccgggatgcg cccgacgagg gccggcgcga
     2101 tccagctcat ggcggccagg tcccgccgca ccagcgactg aacctggggc cgctggacct
     2161 tgaccacgac gggttcgccg gagctgagca ccgcggcgtg cacctgggcg atcgaggcgg
     2221 ccgcgagggg cgctcgctcg aaggagcgga acacggcctc gagcggccgg cccaggtcgg
     2281 cctccacgat ggcgcgcacc agctcgaacc gttcggccgg aacgcggtcg cgcagcaacc
     2341 ggaactggcc gaccagctcc tcggggaaga tcccctcgcc ggacgagatg atctggccca
     2401 gcttgatgta cgtcggcccc agccgctcga aggcccgccg gaggcggcgg gacagcccgg
     2461 accgcgacgt cgactggtcg cccgtgtgcc gttcgatcag gtaccagccg gccagggcga
     2521 gcccgagacg ggccccgacg gtcagcaccc gccctccggg cgggacccgt cgccggcggg
     2581 tgagggccgg cacctc
//
LOCUS       DASWGG010000184         4286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3085253, whole genome shotgun sequence.
ACCESSION   DASWGG010000184 DASWGG010000000
VERSION     DASWGG010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3085253"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2043)
                     /locus_tag="VF954_03345"
     CDS             complement(<1..2043)
                     /locus_tag="VF954_03345"
                     /inference="COORDINATES: protein motif:HMM:NF039983.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HEX9530147.1"
                     /translation="MTEPLTLGQLVEVADVETVVRLDGTAGRLAQLVLTGDVVQSLTA
                     VLAAAGDSGGVAGAGFFVVGPFGSGKSHFLAAVGEALSDPAAARAQVGADPGLAEHLS
                     RARPATVVAVPLIEYRAHARLEDVVAERAARAVGLASPPAGDDRRAAWDGLLAAAQAA
                     RPDRPALVVLLDELSEFLRAKQGPALTEDLRFLQFLGEWSRDQPVIVVAALQEAIEEV
                     ANVSQRELGRIRDRYRPSLTLSMRHVEDLVRGRLVTLRPGAEAHIEAVWRELGRAFPA
                     SPVPLERFMRSYPLHPETLSVLEGLRFVLSQQRGIVDFICHRVRQALDRPAAALLAPD
                     EVFDHFRGRLHERTETARLADIVVPYFERALPELVDDADRSTALRAVKLLCLLSASPL
                     ERSRSAAELAFMLLVRVSELDPGANASYLEAAVLGPIANHGAYLVARPGPSATYAIEL
                     GADAALVLQARLAQVRAELAAGDRRLVATLVRLGSSAVLPLHLMGEVGPTRRELLWQN
                     TQRAVFVATARVLELDPAELDRMVVQARGAGAEGCFVVGEVEVEEAAAAAERAAALVL
                     AAAPVAIWVPAPLQADELDALLDVHARALVSDQAEVEGHHDLLGVLARSAEADAAVAR
                     ELLRRLYFEGRLVCSPPETAGAAGGTLDLTARPAGPDLPSLAGMSFERQLPALVDPL"
     gene            complement(2040..3098)
                     /locus_tag="VF954_03350"
     CDS             complement(2040..3098)
                     /locus_tag="VF954_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9530148.1"
                     /translation="MATPQLEEALALKRSGDLDGAVIAIEGHLGRHPADAVGLAHLAD
                     VQLRRDRRQEAAAALDRAEERSGTTRFTARLRGDLAFREERWVDAAKAYEVADALGDG
                     GTWSLLRLGRCRLRLKDLAGAKGVALRAADREPDGAGAWVLLGDIATREGQLDDAEVM
                     YDRAVHRATGDELAYARLVEVRLLKLPEESRQREVEILLKSSGGANRHLMGVLAKVRR
                     QQGDDDAAADAWEQRARQGDVFARKMQGFALRRAGRLEEAAALLGPCLVAQPDDLILF
                     RTYVGLQRKRAALDELRATLQAALPGAAARKGAYFGELRKLPEPATAPDGSASSDQPA
                     VSDHPAPSDHPAPSTTGT"
     gene            3481..>4286
                     /locus_tag="VF954_03355"
     CDS             3481..>4286
                     /locus_tag="VF954_03355"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9530149.1"
                     /translation="MTSYLITGGAGFIGSHLCEALVNAGDECVVLDDLSTGISANLVA
                     VEQSTRLRFVHGSVLDELAVDDLVHKVDAVVHLAAAVGVRLIIEQPLRSLRTNIRGSE
                     IVFEAAHRHRKKILLASTSEVYGKSPDIPLSEDADRVLGSPRIARWAYSTAKTVDEIL
                     AYAYEKDRGLDAIVVRLFNTIGPRQTAAYGMVVPRLVDQALSDRPLTVFGDGLQTRCF
                     CHVHDVVDAIVLLLRYDRTGESPFNVGSQEEVSILDLAKRIRILAGSSST"
BASE COUNT          649 a         1494 c         1544 g          599 t
ORIGIN      
        1 cagagggtcg acgagagccg ggagctggcg ctcgaagctc atgccggcga gcgacggcag
       61 gtcgggcccg gccgggcgcg ccgtgaggtc gagggtccca cccgcggcgc cggcggtctc
      121 gggcggggag cacaccagcc ggccctcgaa gtacagccgg cgcagcagct cgcgggccac
      181 ggcggcatcg gcttccgcgc tgcgggccag cacaccgagc agatcgtggt ggccttcgac
      241 ctcggcctgg tcggacacca gggcgcgggc gtggacgtcg aggagggcgt cgagctcgtc
      301 ggcctgcaac ggtgccggga cccagatggc gacgggcgcg gcggcgagga ccagggcggc
      361 ggcgcgttcg gcggcggcgg cggcctcttc gacctcgacc tcgccgacga cgaagcagcc
      421 ttcggctccg gcgccccggg cttggacgac catacggtcc agctcggcgg gatcgagctc
      481 gaggacacgg gcggtggcga cgaagacggc gcgctgggtg ttctgccaga gcagctcgcg
      541 ccgcgtcggc ccgacctcgc ccatcaaatg cagcggcagg accgcggacg agcccagccg
      601 cacgagtgtg gcgaccaggc ggcgatcgcc cgcagccagc tcggcgcgaa cctgggccag
      661 gcgggcctgg agcaccaggg cggcgtcggc gccgagctcg atggcatagg tcgcggacgg
      721 gccggggcgg gcgacgaggt aggcgccatg gttggcgatg ggcccgagga cggccgcttc
      781 caggtaggag gcgttggccc cggggtcgag ctcgctgacc cgaaccagca gcatgaaagc
      841 cagctcggcc gccgagcggg accgctcgag cggcgaggcc gagaggaggc agagcagctt
      901 cacggcgcgc agggcggtgc tgcggtccgc gtcgtcgacc agctccggaa gggcccgctc
      961 gaagtacgga acgacgatgt cggccagccg cgccgtctcg gtgcgctcgt gcagccggcc
     1021 ccggaagtgg tcgaagacct cgtccggtgc cagcagggct gccgcgggcc ggtcgagggc
     1081 ctggcgcacg cggtgacaga tgaagtcgac gatcccgcgc tgctgcgaga ggacgaacct
     1141 cagaccctcg agcaccgaga gcgtctcggg gtgcagcggg tagctccgca tgaagcgctc
     1201 gagcggcacg gggctggcgg ggaaggcccg gcccagctcc cgccacacgg cttcgatgtg
     1261 ggcctccgcg cccgggcgga gggtgaccag gcggccgcgc acgagatcct cgacgtggcg
     1321 catcgacagc gtcaggctgg gccggtaacg gtcgcgtatg cggcccagct cgcgctggct
     1381 gacgttggcc acctcctcga tcgcctcctg gagggcggcc actacgatga cgggctggtc
     1441 ccggctccat tcgccgagga actggaggaa ccggaggtcc tcggtcaacg ccggcccctg
     1501 cttggcccgc aggaactcgc tgagctcgtc gagcaggacg accagcgccg ggcggtccgg
     1561 ccgggccgcc tgggccgcgg cgagcagccc gtcccacgcg gcgcgccggt cgtcgccggc
     1621 cggaggcgac gccagcccga cggcccgggc cgcccgctcg gccaccacgt cctcgagccg
     1681 ggcgtgcgcc cggtactcga tgagcggcac ggccacgacc gtcgccggcc gggcccggct
     1741 caggtgctcc gccaatcccg ggtcggcgcc cacctgggcc cgggccgcgg cggggtccga
     1801 cagggcctcg cccacggcgg ccaggaagtg cgacttgccc gagccgaacg ggccgaccac
     1861 gaagaagccc gcgccggcca cccctcccga gtctcccgcg gcggcgagca cggccgtcag
     1921 gctctgcacg acgtcgccgg tgagcaccag ctgcgccaac cggcccgcgg tgccgtcgag
     1981 gcgcacgacg gtctccacgt cggccacctc gacgagctgg ccgagcgtca gcggctcggt
     2041 cacgtcccgg tggtcgaggg ggcggggtgg tccgaagggg cggggtggtc cgacacggcc
     2101 ggctggtccg aggaggccga tccgtcagga gccgtggccg gctcggggag ctttcgcagc
     2161 tcgccgaagt aggcgccctt gcgggccgcc gcgcccggca gggcggcctg caacgtggcc
     2221 cgcagctcgt ccagcgcggc ccgcttgcgc tggagcccga cgtaggtgcg gaacaggatc
     2281 aggtcgtcgg gctgggccac caggcacggg cccaggaggg cggcggcctc ctccagccgg
     2341 ccggcccggc ggagcgcgaa gccctgcatc ttgcgggcga acacgtcgcc ctgccgggcc
     2401 cgctgctccc acgcgtcggc cgcggcgtcg tcgtcgccct gctggcgacg gaccttggcc
     2461 agcacgccca tgaggtgccg gttggccccc ccgctcgact tcaggaggat ctcgacctcg
     2521 cgctggcggc tctcctcggg aagcttcagc agccggacct cgaccagccg ggcatacgcc
     2581 agctcgtcgc cggtcgcccg atgcacggcc cggtcgtaca tgacctcggc gtcgtcgagc
     2641 tggccctcgc gggtggcgat gtcgcccagc agcacccatg cgccggcgcc gtcgggctca
     2701 cggtcggcgg cccgcagggc cacccccttg gctccggcca gatccttgag ccgcagccgg
     2761 cagcggccca gccgcagcag ggaccacgtc ccgccgtcgc ccagggcgtc ggccacctca
     2821 taggccttgg ccgcgtcgac ccagcgctcc tcccggaagg ccaggtcgcc ccggagccgg
     2881 gcggtgaacc gggtcgtgcc cgaccgctcc tcggcccggt ctagggccgc ggccgcctcc
     2941 tggcgtcgat cgcgccgcag ctgcacgtcc gccaggtgcg ccaagcccac ggcgtcggcc
     3001 ggatggcggc ccaggtggcc ctcgatggcg atcaccgcgc cgtccaggtc gcccgatcgc
     3061 ttgagggcca gggcctcctc gagttgcggc gtggccacgg aagatcagcc tatgcgcagg
     3121 cggcaggtcg gacgggcgac tgttcatatg tgcggaaatt cgtactgtac cctagtatgc
     3181 cctaggagca cattggtggt tagtcgggtt gcagaacgcc cgaccgttga gccaacgaac
     3241 gcccggtgag cccggcaggc gcccgggccg ctttcaacag atcaagtgcg aggcgttcca
     3301 ctgcggtccg tgatacagaa gtcaggatca acgggcaccg caccactgag ggtccaccgg
     3361 gcgacagctc gaggtatagc gaggtgccca ccctcagatc tgacagcccc tgaagacaac
     3421 cacggccagc gcaccggcag ccaccttcgc gtccatagtc gctaccgcta gccttcgctc
     3481 atgacgagct acctcattac ggggggagca ggcttcatcg gctcgcattt gtgtgaagcg
     3541 ctggtcaacg ctggagacga gtgcgttgta ctagacgacc tctcgaccgg gattagcgca
     3601 aatcttgtcg ccgttgaaca gtccactcgc ctccgatttg tacatgggtc ggtgctagat
     3661 gagctggccg tggatgactt ggttcataag gtcgacgcgg ttgtgcacct ggcggcggca
     3721 gttggcgttc ggttgatcat cgagcagccg ctacgctcac tgcgaacaaa tatccgcggc
     3781 tccgaaatcg tcttcgaggc tgcgcaccgg caccgcaaga agattttact tgctagcacg
     3841 tctgaggtgt atggaaagag cccagatatc ccgctatccg aggatgccga ccgagtgttg
     3901 ggctccccgc gtatcgcgcg ctgggcctat tctacggcaa aaacggtaga cgagatcctc
     3961 gcgtacgcat atgagaaaga ccgcgggctg gacgccattg tggtccggct cttcaacacg
     4021 atcggccctc gacaaaccgc tgcatacggt atggtagttc ctcgccttgt cgaccaggcg
     4081 ctaagcgatc gcccgctaac cgtatttggc gacggcctgc agactcggtg cttttgccat
     4141 gttcacgacg ttgtggatgc aatagttcta ttgctgcgat atgaccgtac aggggagtcg
     4201 cctttcaacg tcggctcgca agaggaggtc agcattctgg accttgccaa gcggatccga
     4261 attctggccg gctcctcgtc taccat
//
LOCUS       DASWGG010000185         5888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1156337, whole genome shotgun sequence.
ACCESSION   DASWGG010000185 DASWGG010000000
VERSION     DASWGG010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156337"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VF954_03360"
     CDS             complement(<1..926)
                     /locus_tag="VF954_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX9530150.1"
                     /translation="MSDQVPYRYERTHAVADVRADFDGRLAPGEESGSVVSVAGRLML
                     LRDQGKMVFAELRDSGGALQVWALSALTEDFEAFSALTLGDWVGATGEVVKTRRGELS
                     VKVAKWTLLAKARSQFGDKWRGVTDTDTRYRQRYVDLWANRGSRDVFVLRSRLVSLLR
                     RWLEDRGFVEVETPIFHPIPGGAAARPFVTHHNALDVDLFLRIAPELYLKRLVVGGFE
                     KVFEIARVFRNEGLSPRHNPEFTMLELYWAYADYRDMMVLVEEMVAQLATELRGTTAL
                     SYGGRPLDLTPPWRRATLAELVEEHTGVALSPR"
     gene            complement(923..1690)
                     /locus_tag="VF954_03365"
     CDS             complement(923..1690)
                     /locus_tag="VF954_03365"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX9530151.1"
                     /translation="MLLAIDVGNTQTVIGLYGVDDSLLYHWRISTVPDRTADEHALLV
                     TQLLDLEGVDDADITGIAVCSTVPHLTAVMREMTARWPSIEAVVVEPGVKTGLPILYE
                     NPREVGADRIANAVAAWEMYGGPSIVVDFSGTATIIDAITGKGEYLGGAIIPGIEISL
                     DALFERAASLRQVELVEPRSAIGRNIVESVQSGALYGFASQVDGLCRRFVAELGPATV
                     IATGSLSALMAPLSECIEYQEPWLTLHGLRLIYRKNT"
     gene            complement(1700..2566)
                     /gene="nadC"
                     /locus_tag="VF954_03370"
     CDS             complement(1700..2566)
                     /gene="nadC"
                     /locus_tag="VF954_03370"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX9530152.1"
                     /translation="MSSGEPVSADAHPPALAVREAVARALAEDLLPMGDLTAGLVPPG
                     TQGRALFVARAEGVLAGRRCAEEALRQVDAGLEARWELDDGSPVKAGAVIGEVRGPLR
                     SILTAERTALNFLCHLSGVATLTRRYVDAVRAASASARVLDTRKTTPGLRALEKAAVR
                     AGGGHNHRGSLSEGVLVKDNHLGGLTITEAVARARDRWPGRMLEVECDRVEQVREAIQ
                     AGATMVLLDNMTPAQVAECAQLPRHPPLEVSGRVGLDDAPRFAMAGADFISVGALTHS
                     APVLDIGLDLGP"
     gene            complement(2563..4158)
                     /locus_tag="VF954_03375"
     CDS             complement(2563..4158)
                     /locus_tag="VF954_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9530153.1"
                     /translation="MTAATPLDVLVLGSGVAGLSAAFRAAGAPGMRVGVLTKGELSQS
                     ATRWAQGGVATVLGGDEDSTDLHVSDTLAAGAGLCDADAVRILVDEGPRRVNELIALG
                     AVFDRGPEGTLLLAREGGHSTARVVHAGGAATGAEIERALVEAVRRTAASVLEHWFAL
                     ELIVEGGRCRGVLAVGPDGTLAEVRAAHVICATGGAGQLFAVTTNPAEATADGLAMAI
                     RAGVAVADVEFMQFHPTALHHPAMPRPLLSEALRGHGALLRDARGERFVDELQPRDVV
                     SRAMMARMFAQEVDHLWLDATGLREFDAWFPTIAASVRSIGLDPGRDWLPIAPAAHYL
                     SGGVVTDLDGASAVPGLWACGEVACTGVHGANRLASNSLLEGMVFAPRVVEAIDRGKD
                     HAEPTGAMRSVLAGQSRWVALPPGPPVAGAGRVARGPGASAAPPGPAPDPVKGRDQLQ
                     RAMTSGAGVLRSAASLDVTGAVLEDLDRPGTDRDGWELANLVLAGRALVHTARTREES
                     RGAHTRTDFPATAPHWQVRIVVA"
     gene            complement(4169..4528)
                     /gene="panD"
                     /locus_tag="VF954_03380"
     CDS             complement(4169..4528)
                     /gene="panD"
                     /locus_tag="VF954_03380"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX9530154.1"
                     /translation="MRRRMMKSKIHRATVTDANLNYVGSISLDAGLMAMADIREWEQV
                     AVLDIDNGARFETYAIPGGPGQVCLNGAAARLVQPGDKVIVITYADYEDAELEGFAPR
                     VVHVDERNQAALAAAWP"
     gene            complement(4539..5411)
                     /gene="panC"
                     /locus_tag="VF954_03385"
     CDS             complement(4539..5411)
                     /gene="panC"
                     /locus_tag="VF954_03385"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX9530155.1"
                     /translation="MRLITTVAEMAQAAEIGRKRGNVGFVPTMGFLHAGHESLIRRAA
                     RECDLTAVSIFVNPLQFGAGEDLAAYPRDLDRDLELAERSGAELAFVPPTEEMYPRPM
                     AATVSVQGVGDAWEGESRPTHFAGVATVVAKLFAIVGPSRAYFGDKDFQQLAVIRRMA
                     GDLSLPVEVVGCPTVRDADGLALSSRNIYLSPAERQAAPVLYRALLAGRRRVQEAGER
                     APAAVEDAMRAVVAAEPLAALDYAAAVGASDLSRPERLSGEVRLLIAARVGPARLIDN
                     LGTLSPNPSATSVT"
     gene            complement(5402..>5888)
                     /locus_tag="VF954_03390"
     CDS             complement(5402..>5888)
                     /locus_tag="VF954_03390"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX9530156.1"
                     /translation="RRAAVHPLLALPEPEVGARLLRSGAWFGVGGDPAAADLVAALGG
                     RAVPVADEDRAAYHAAACVASNLLVALLDQAYRLAAQAGMPPAALLDLVRGTVDNVAA
                     LGPAGALTGPVARGDWATVESHRRAVAALGDADLAGYDAMAALAGRLAGHRAGVEVPC
                     A"
BASE COUNT          787 a         2167 c         2206 g          728 t
ORIGIN      
        1 cggggactga gggccacgcc ggtgtgttcc tcgaccagct cggcaagcgt ggcccgtcgc
       61 catggcgggg tgaggtcgag cggccggccg ccgtaggaga gcgcggtggt gccacgcagc
      121 tcggtggcca gctgcgccac catctcctcg accaggacca tcatgtcccg gtagtcggcg
      181 taggcccagt acagctcgag catcgtgaac tcggggttgt ggcggggcga gaggccttcg
      241 ttgcggaaga cgcgggcgat ctcgaagacc ttctcgaagc cgcccaccac cagccgcttg
      301 agatacagct cgggcgcgat gcgcaggaag aggtcgacgt cgagggcgtt gtggtgggtg
      361 acgaagggcc gggccgcggc gccgccgggg atggggtgga agatcggcgt ctccacctcc
      421 acgaagcccc ggtcctcgag ccagcgccgc agcaggctca ccagccggct gcgcagcacg
      481 aagacgtccc gggaacccct gttggcccac aggtccacgt accgctgcct gtagcgggtg
      541 tcggtgtcgg tgaccccccg ccacttgtcg ccgaactggc tgcgcgcctt ggccagcagc
      601 gtccacttcg ccaccttgac cgacagctcg ccccggcgcg tcttgaccac ctcgcccgtg
      661 gcgccgaccc agtcgccgag cgtcagcgcg gagaacgcct cgaagtcctc ggtgagggcg
      721 ctgagggccc acacctggag ggcgccgccc gagtcgcgca gctcggcgaa gaccatcttg
      781 ccctggtccc tgagcaacat gaggcggccg gcgaccgaca ccaccgagcc cgactcttcg
      841 cccggggcca gccgaccgtc gaagtcggcg cggacgtcgg ccacggcgtg ggtgcgctcg
      901 tagcggtacg ggacctggtc gctcacgtgt tctttcggta gatgagccgc aggccgtgca
      961 gggtgagcca cggctcctgg tactcgatgc actccgacag cggcgccatc agggcgctga
     1021 ggctcccggt cgcgatgacc gtagccgggc ccagctcggc cacgaaccgc cggcacaggc
     1081 cgtcgacctg ggaggcgaag ccgtacaggg cgcccgactg cacggactcg acgatgttgc
     1141 ggccgatggc cgagcggggc tcgaccagct ccacctgccg gagggaggcg gcccgctcga
     1201 agagggcgtc caggctgatc tcgatgcccg ggatgatggc cccgccgagg tactcccctt
     1261 tcccggtgat ggcgtcgatg atcgtcgccg tgccggagaa gtcgacgacg atggacggcc
     1321 cgccgtacat ctcccaggcg gccacggcgt tggcgatgcg gtccgcgccg acctcacggg
     1381 ggttctcgta caggatgggc agcccggtct tgaccccggg ctcgacgacc acggcctcga
     1441 tgctgggcca ccgggcggtc atctcgcgca tcacggcggt caggtggggg accgtcgagc
     1501 acaccgcgat cccggtgatg tcggcgtcgt cgacaccctc gaggtcgagc agctgggtga
     1561 ccaggagggc gtgctcgtcc gccgtgcggt cgggcacggt cgagatccgc cagtggtaga
     1621 gcagggagtc gtcgaccccg tacagcccta tgacggtctg ggtgttgccg acgtcgatgg
     1681 cgagcagcac ggccgtgcct cagggcccga ggtcgaggcc gatgtcgagc accggcgcgg
     1741 agtgggtgag ggcgccgacc gagatgaagt cggcgccggc catcgcgaac cggggggcgt
     1801 cgtcgagccc gacccggccc gagacctcca gtggaggatg gcgggggagc tgggcgcact
     1861 cggccacctg tgcgggggtc atgttgtcga gcaggaccat ggtggcgccg gcctggatcg
     1921 cctcgcgcac ctgctccacg cggtcgcact ccacctccag catccgcccc ggccagcggt
     1981 cccgggcccg ggccacggcc tcggtgatcg tcaggccgcc caggtggttg tccttcacga
     2041 gcacgccctc ggacaggctg ccgcggtggt tgtggcctcc cccggcccgg acggcggcct
     2101 tctcgagcgc ccgcagcccg ggcgtcgtct tgcgtgtgtc gaggacgcgc gcgctcgcgc
     2161 tggcggcccg gaccgcgtcg acgtagcggc gggtgagggt ggccaccccc gagaggtggc
     2221 agaggaagtt cagcgcggtc cgctccgcgg tgaggatcga tcggagcggg ccgcgaacct
     2281 cgccgatcac ggcgccggcc ttcaccgggg agccgtcgtc cagctcccac ctcgcttcca
     2341 gcccggcgtc gacctggcgc agggcctcct ccgcgcagcg ccggccggcc agcaccccct
     2401 cggcccgggc cacgaacagg gcccggccct gcgtcccggg cgggaccagc ccggccgtga
     2461 ggtcgcccat gggcagcagg tcctcggcca gggcccgggc cacggcctcc cgcaccgcga
     2521 gggcgggggg atgggcgtcg gcgctcaccg gctcgccgct gctcacgcca ccacgatccg
     2581 gacctgccag tggggggcgg tggcggggaa gtcggtccgg gtgtgggcgc cgcggctctc
     2641 ctcgcgggtt cgggcggtgt gcaccagggc gcggccggcg agcaccaggt tggccagctc
     2701 ccacccgtcg cggtcggtgc ccggccggtc gaggtcctcc aggacggcgc cggtgacgtc
     2761 caggctggca gccgagcgca ggacgcccgc tcccgaggtc atggcccgct ggagctggtc
     2821 ccggcccttg accgggtcgg gcgcggggcc agggggggcc gcggaggctc ccgggcctcg
     2881 ggcgaccctg cccgccccag cgaccggcgg gccgggcggg agggccaccc accggctctg
     2941 cccggccagc accgagcgca tggcgccggt gggctcggcg tggtccttgc cccggtcgat
     3001 ggcctcgacc accctgggcg cgaagaccat gccctcgagc agcgagttcg aggccagccg
     3061 gttggcgccg tggacacccg tgcaggccac ctcgccacac gcccagaggc cgggaacggc
     3121 gctggccccg tcgaggtcgg tgacgacacc gccggagagg tagtgcgcgg ccggggcgat
     3181 cggcaaccag tcccggcccg ggtccaggcc gatggagcgc accgaggccg ctatcgtcgg
     3241 gaaccacgca tcgaactccc gcagcccggt ggcgtccagc cacaggtggt cgacctcctg
     3301 ggcgaacatg cgcgccatca tggcccggct caccacgtcg cggggctgga gctcgtcgac
     3361 gaaccgctcc ccccgggcgt cccgcagcag cgccccgtgg ccgcgcaggg cctcggacag
     3421 cagcggccgg ggcatggccg ggtggtgcag ggccgtgggg tggaactgca tgaactccac
     3481 gtcggcgacg gccacccccg cccggatggc catcgccagg ccgtcagccg tggcctccgc
     3541 cgggttcgtc gtgaccgcga agagctgccc ggcgcccccg gtggcgcaga tgacatgcgc
     3601 cgcccgcacc tcggcgaggg tcccgtcggg accgacggcc agcacccctc ggcatcggcc
     3661 accctcgacg atcaactcga gggcgaacca gtgctcgagc accgaggcgg cggtgcggcg
     3721 cacggcttcg acgagggccc gctcgatctc ggcgcccgtg gccgccccgc cagcgtgcac
     3781 gacgcgcgcc gtggagtggc cgccctcccg agccaggagg agcgtcccct ccgggccccg
     3841 gtcgaacacc gccccgagcg cgatcagctc gttgacccgc ctcggcccct cgtcgacgag
     3901 gatccgcacg gcgtccgcgt cgcagaggcc ggccccagcc gccagcgtgt cggacacgtg
     3961 caggtcggtg gagtcctcgt cgccgccgag gaccgtcgcc acccctccct gggcccagcg
     4021 ggtggccgac tggctgagct cgcccttggt cagcacgccc acccgcatgc cgggcgcgcc
     4081 ggcagcccgg aaggccgccg acaggccggc gacgccgctg cccagcacga gcacgtcgag
     4141 aggcgtggcg gcggtcaccg catcgaggct acggccaggc ggcggccagc gcggcctgat
     4201 tgcgctcgtc cacatggacg acgcgggggg cgaaaccctc cagctcggcg tcctcgtagt
     4261 cggcgtaggt gattacgatc accttgtcgc ccggctgcac gaggcgcgcc gccgctccgt
     4321 tgaggcagac ctggccgggc ccgccgggga tggcataggt ctcgaagcgc gccccgttgt
     4381 cgatgtcgag cacggccacc tgctcccact cgcggatgtc ggccatggcc atgaggccgg
     4441 cgtcgagcga gatcgagccg acgtagttca ggttggcgtc ggtgactgtc gcccggtgga
     4501 tcttggactt catcatgcgg cggcgcataa ctcgctcctc acgtgaccga tgtggctgaa
     4561 gggttggggg aaagggtgcc gaggttgtcg atcaggcggg cgggcccgac ccgggccgcg
     4621 atgagcagcc ggacctcacc ggacagccgc tccggccgcg acaggtcgga cgcgcccacc
     4681 gcggcggcgt agtcgagggc ggccagcggc tcggcggcga ccaccgcgcg catggcatcc
     4741 tccacggcgg cgggcgcccg ctcgcccgcc tcttggaccc gccggcggcc cgcgagaagg
     4801 gcccggtaca gcaccggcgc ggcctgccgc tcggccgggc tgaggtagat gttgcggctg
     4861 gaaagggcga ggccgtcggc gtcccggacg gtggggcagc ccacgacctc gacgggcagc
     4921 gagaggtccc ccgccatgcg ccggatgacg gcgagctgct ggaagtcctt gtccccgaag
     4981 taggcgcggc tgggcccgac gatggcgaac agcttggcca ccaccgtcgc cacgcccgcg
     5041 aagtgggtgg gccgggactc gccctcccag gcgtcaccga cgccttgcac gctgacggtc
     5101 gcggccatcg gccgggggta catctcctcg gtcggcggga cgaaggccag ctcggcgccc
     5161 gaccgctcgg ccagctccag gtcccgatcg aggtcccggg gataggccgc caggtcctcg
     5221 ccggcgccga actgcagcgg gttgacgaag atgctcaccg ccgtgaggtc gcactcccgg
     5281 gcggccctac ggatgagcga ttcgtggccc gcgtgcagga accccatggt cggcacgaac
     5341 ccgacgttgc cacgcttgcg gccgatctcc gcggcctgcg ccatctccgc cacggtcgtg
     5401 atcaggcgca cgggacctcg acccccgccc ggtggccagc cagccgcccg gccaacgcgg
     5461 ccatggcgtc gtagccggcc aggtccgcat cgccgagggc ggcgacggcc cggcggtggg
     5521 actccacggt cgcccagtcg ccccgggcca ccgggccggt gagggcgccg gccggcccga
     5581 gcgccgccac gttgtcgacc gtgccccgca ccaggtcgag gagcgccgcc ggcggcatcc
     5641 cggcctgcgc cgccagccgg taggcctggt cgaggagggc gaccaggagg ttggaggcca
     5701 cgcaggcggc ggcgtggtag gcggcccgat cctcgtcggc gaccggcacg gcgcgcccgc
     5761 ccagggcggc gaccagatcg gcggcggcgg ggtcgccgcc caccccgaac cacgcccccg
     5821 agcgcagcag gcgggccccg acctcgggct cgggcagggc gaggagcggg tgcaccgcgg
     5881 cgcggcgg
//
LOCUS       DASWGG010000186         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2760950, whole genome shotgun sequence.
ACCESSION   DASWGG010000186 DASWGG010000000
VERSION     DASWGG010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2760950"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1595
                     /locus_tag="VF954_03395"
     CDS             <1..1595
                     /locus_tag="VF954_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX9530157.1"
                     /translation="PRSTPAGAGSCGGRSVVLGPVALQVLISRLTGVAEEMGAVLRRA
                     AYSPNIKERADCSAALFTPGGELLVQAEHIPVHLGSMPASVAAAIASLGRDVRAGEHV
                     ILNDPFAGGTHLNDITVVTPCFVDASLVGWAANRAHHADVGGMAPGSIPPEATEIQQE
                     GLRIPPVGLTTEVRALLIAASRTPEERVGDLDAQVGANVVGARRLAEIAASGAPLREV
                     LDYGERRMRAALAEVPDGCFRFADRLDSCGPRPEQQRPATIAVAVTVDGDEITFDFTG
                     TDAQRPGNVNAVEAVTVSAVAFAVRSVTDPTIPPSGGALRPVHVIAPAGTIVAARPPA
                     AVGAGNVEVSQRVADVCLGALSQAAPDRVPAAGQGTMNNLILGGAGWVYYETIGGGQG
                     GRPGGPGRPPRPGQSAMQVGMTNTRNTPAEALERAYPLRVRRYAIRRGSGGAGLAPGG
                     DGIERELEVLEDCTLSLITERRVSRPWGLAGGEPGAVGENWLLPAGDGSRAERLPDKC
                     TVALRAGDVVRILTPGGGGWGR"
     gene            1607..>2000
                     /locus_tag="VF954_03400"
     CDS             1607..>2000
                     /locus_tag="VF954_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9530158.1"
                     /translation="MRYVETRGARLSVIGLGTWQFGSREWGYGAGYASGEAIEIVRRA
                     VELGINLIDTAEIYGFGRSERIVGRAIRGLRPHVFVATKVFPVLPLAPVVAHRGRASA
                     ARLGIKPIDLYQVHWPNPVVPLSTTMHGI"
BASE COUNT          254 a          757 c          733 g          256 t
ORIGIN      
        1 cgccgaggtc cacgccggcg ggagctggct cctgcggagg gcgtagcgtg gttctgggcc
       61 cggtcgccct ccaggtgctg atctcccggc tgaccggcgt ggccgaggag atgggggcgg
      121 tcttgcgccg ggccgcgtac agcccgaaca tcaaggagcg ggccgactgc tcggccgcgc
      181 tgttcacccc cggcggcgag ctgctggtcc aggccgagca catccccgtc cacctcggct
      241 cgatgcccgc gtccgtggcc gccgccatcg cctctctcgg ccgggacgtg cgagccggcg
      301 agcacgtgat cctcaacgac ccgttcgcgg gaggcaccca cctcaacgac atcaccgtcg
      361 tcacgccctg cttcgtcgac gcgtcgttgg tgggctgggc cgccaaccgg gcccaccacg
      421 ccgatgtggg cggcatggcc cccggctcga tcccgcccga ggccaccgag atccagcagg
      481 agggcctgcg catcccgccc gtgggcctca cgaccgaggt tcgcgccctg ctgatcgccg
      541 cgtcccgcac ccccgaggag cgcgtcgggg acctcgacgc ccaggtgggc gccaacgtgg
      601 tcggcgcccg gcgcctggcc gagatcgcag cgagcggggc gcccctgcgc gaggtcctgg
      661 actacgggga gcggcgcatg cgggccgcgc tcgccgaggt gcccgacgga tgctttcgct
      721 tcgcggaccg gctggactcc tgcgggcccc ggcccgagca gcaacggccc gccacgatcg
      781 ccgtcgccgt gaccgtcgac ggcgacgaga tcaccttcga cttcacgggc accgacgccc
      841 agcgcccggg caacgtcaac gcggtcgagg ccgtgacggt cagcgcggtg gccttcgccg
      901 tgagatccgt caccgacccg accatcccgc caagtggcgg cgcgctccgg cccgtgcacg
      961 tgatcgcccc ggccgggacg atcgtcgcgg cccggccacc ggccgcggtc ggtgccggca
     1021 acgtcgaggt cagccagcgg gtggcggacg tgtgcctcgg ggcgctgagc caggccgcgc
     1081 ccgatcgggt ccccgccgcg ggccagggaa ccatgaacaa cctcatcctc ggcggagcgg
     1141 gctgggtcta ctacgagacg atcggcgggg ggcagggcgg acggcccggc gggccgggcc
     1201 gcccaccccg ccccgggcag agcgccatgc aggtcggcat gaccaacacc cgcaacaccc
     1261 cggcggaggc gctggagcgg gcctatcccc tgcgggtgcg ccgctacgcc atccggcggg
     1321 gaagcggggg cgccggcctg gcccccggtg gcgacgggat cgagcgtgag ctcgaggtcc
     1381 tcgaggactg caccctgtcg ctcatcaccg agcggcgggt gagccgcccg tgggggctcg
     1441 caggcggcga gccgggcgcg gtcggtgaga actggctcct ccccgctggc gacgggtcga
     1501 gggccgagcg gcttccggac aagtgcacgg tcgcgctccg cgccggcgac gtggtgcgca
     1561 tcctcacgcc cggcgggggc ggttggggtc ggtaggatcg ctcgccgtgc gctacgtcga
     1621 gaccagaggg gcccggctct ccgtcatcgg gctcgggacg tggcagttcg gctcgaggga
     1681 gtggggttac ggcgccggtt acgcctccgg cgaagccatc gagatcgtcc gccgcgctgt
     1741 ggagctcggc atcaacctga tcgacacagc cgagatctac ggcttcgggc gctccgagcg
     1801 catcgtcggc cgcgccatcc gcggcctgcg ccctcacgtc ttcgtggcga ccaaggtctt
     1861 ccccgtcctg cccctcgccc cggtcgtggc gcaccggggc cgcgccagcg cggcgcgcct
     1921 gggcatcaag cccatcgacc tctaccaggt ccactggccc aatccggtcg tgccactcag
     1981 cacgacgatg cacggcatcc
//
LOCUS       DASWGG010000187         3412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3087571, whole genome shotgun sequence.
ACCESSION   DASWGG010000187 DASWGG010000000
VERSION     DASWGG010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3087571"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VF954_03405"
     CDS             complement(<1..428)
                     /locus_tag="VF954_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX9530159.1"
                     /translation="MFERFTDRARRVLVLAQEEARLLNHNFIGTEHILLGLIHEGEGV
                     AAKALESLGISLEAVREKVEETIGPAGSSTTGSPPFTPRAKKVLELSLREALQLGHNY
                     IGTEHMLLGLVREGEGVAAQVLVNLGADLSRVRQQVIQLLS"
     gene            552..1535
                     /locus_tag="VF954_03410"
     CDS             552..1535
                     /locus_tag="VF954_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9530160.1"
                     /translation="MNVAGLLGLPRLDQDLDRLEEALRGSVGSEDPFLSEVAGHLVAA
                     GGKRLRPALTVAAAAAGGAGGDGAGEEVLRGGVSVELVHLGSLYHDDVMDEALTRRNV
                     ESVNARWGNLVAIVAGDFLLARASELAASLGTEVAGLLAATIARLCEGQVLEVRSTFD
                     PGRTEPAYLASIAGKTAALMASACRIGALTAGLARDQVDAVTAFGSAFGMVFQIRDDI
                     LDVAATDAALGKPTGQDLVQGVYTLPVLRALGDPDVGPELRALLGSPLQPGEREKARA
                     MVRSTPALESSLATARAYAGQAGAAARRVGRGPVVDAMAELGHRILDDLPI"
     gene            complement(1683..2369)
                     /locus_tag="VF954_03415"
     CDS             complement(1683..2369)
                     /locus_tag="VF954_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530161.1"
                     /translation="MTASPGVRGALGAAAATLRRRALGRFEIDEWGADPELVDLLVPA
                     LGAVTRIRVHGATRVPERGPALLVVGSRLGLAEPFVVVAGLRRAAGRRARFAGIPDVA
                     PIGLVLRRLGGAVGRPGEVAGLLHAGHLVLVTLSPEWRRPLRAGTVEPELIHPALGAG
                     APILPVAAMGGEISGRWQVVVGEPIAPPRRRTGRHSPLALAELADAARAGVQELIDEA
                     SPPGPSWLGR"
     gene            complement(2366..3331)
                     /locus_tag="VF954_03420"
     CDS             complement(2366..3331)
                     /locus_tag="VF954_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9530162.1"
                     /translation="MRVLVTGVGGDLGRKVALLLAADPGVSDLVGTDMYPPRGRVRNM
                     TFRLIDPRDRRPLLRLVREVEPTAIVHLGIYEPSARSTPHEAWERTESATLAVLGEAA
                     DLGALERVVLRSGLEVYGRGRGSVTVPDEAVPPVPTSAFGRSLLEVERLAFATGQDGG
                     VPVAALRFAPLVGPRFPSPLGRLLRLPVVPFSALADPPFSVVHADDAAAAVVAALRAG
                     IDGPLNVVAPGAVTVSQAALMGRRLPLGLLGPEWALVRRLTAAAGAPLPSHLMELLHR
                     GRTACGARCRELVPAIAERTTADIVAELHGWSDPARLQLVQGEAA"
BASE COUNT          442 a         1230 c         1320 g          420 t
ORIGIN      
        1 gagaggagct ggatcacctg ctggcggacc cgggacagat cggcccccag gttgaccagc
       61 acctgggcgg ccacgccctc gccctcgcgc accagcccga ggagcatgtg ctcggtgccg
      121 atgtagttgt ggcccagctg gagggcttca cgcagcgaca gctccaggac cttcttggcc
      181 cggggcgtga acggcggtga gcccgtcgtg gacgagccgg ccggaccgat ggtctcctcg
      241 accttctcgc gcacggcctc cagcgagatg ccgagcgact cgagcgcctt ggccgccacc
      301 ccctcgccct cgtggatgag gccgagcagg atgtgctcgg tgccgatgaa gttgtggttg
      361 aggaggcggg cctcctcttg agcgaggacc aggaccctgc gggcccggtc cgtgaaacgt
      421 tcgaacaagt ggtccgcctc ctaggacggc gctgtgtgtg aaagccagtc taccccggtt
      481 ggtgtgcggt taggcattgg ggtggtgccg gattcttggt tgctgtatcg gtcgcctaac
      541 gttctgcctt agtgaacgtc gccgggctgt tgggccttcc ccgactcgac caggacctcg
      601 atcgcctcga ggaggcgttg cgtgggtcgg tggggtccga ggacccgttc ctgtcggagg
      661 tggcgggtca cctggtcgcc gccgggggca agcggctgcg gccagccctg acggtggccg
      721 cggccgcggc aggtggggcc ggtggggacg gcgccggcga ggaggtgctc cggggcggcg
      781 tctcggtcga gctggtgcac ctgggctcgc tgtaccacga cgacgtgatg gacgaggccc
      841 tcacccgccg gaacgtggag agcgtcaatg cccgctgggg gaacctggtg gccatcgtgg
      901 ccggtgactt cctgctggcc cgggcgtcgg agctggccgc ctccctcggc acggaggtgg
      961 ccggcctgct ggccgccacc atcgcccggt tgtgcgaggg gcaggtgctg gaggtgcggt
     1021 cgacgttcga cccgggccgg acggaaccgg cctacctggc ctcgatcgcc ggcaagaccg
     1081 ccgccctgat ggcctcggcc tgccggatcg gggcgctcac ggccgggctg gcgcgggacc
     1141 aggtcgatgc ggtgacggcg ttcggatcgg ccttcgggat ggtcttccag atccgcgacg
     1201 acattctgga cgtggccgcc accgacgccg ccctgggcaa gccgaccggc caggacctgg
     1261 tccagggcgt gtacaccctc ccggtgctgc gggccctcgg cgaccccgac gtgggtcccg
     1321 agctgcgggc cctgctgggc agcccgctcc agccgggcga gcgggagaag gcccgggcca
     1381 tggtgcgctc cacgcctgcg ctggagtcct cgctggccac cgcccgggcc tacgccggtc
     1441 aggcgggcgc ggcggcgcgg cgcgtgggcc gcggcccggt ggtggacgcc atggccgagc
     1501 tgggccatcg catcctggac gacctgccca tctgatccca cccaattaag gagcctttgg
     1561 cattcttcca tgcctttatg tcgctatagc gagttttttg cttgaagaaa ggtcggcccg
     1621 gtgcgctcag cgccccagcc aggacgggcc ggggcgggcg ccgacggcgg gaccggttgc
     1681 gctcagcgcc ccagccagga cgggcccggc gggctggcct cgtcgatcag ctcctgcacg
     1741 ccggcccggg cggcgtcggc cagctcggcc agcgccagcg gcgagtggcg gccggtccgc
     1801 cggcggggcg gggcgatggg ctcgcccacg accacctgcc accggccgct gatctccccg
     1861 cccatggcgg ccacgggcag gatcggggcg ccggcaccca gggcgggatg gatgagctcg
     1921 ggctcgaccg tgcccgctcg cagcggccgg cgccactcgg gcgagagcgt caccagcacg
     1981 aggtgaccgg catggagcag gccggcgacc tcgccggggc ggccgaccgc cccgccgagc
     2041 ctgcgcagca ccaggccgat gggggcgacg tccgggatgc cggcgaaccg ggcccgccgg
     2101 ccggcggcgc gccggagccc ggcgaccacg acgaacggct cggccaggcc cagccggctg
     2161 ccgacgacca gcagggccgg gccccgctcg ggcacgcggg tggcgccgtg cacccggatc
     2221 cgggtgaccg ccccgagggc cgggacgagc aggtcgacga gctcggggtc ggcgccccat
     2281 tcgtcgatct cgaagcggcc cagcgcccgc cggcgcaggg tggcggccgc cgcgcccagc
     2341 gcgccgcgga cgccggggct cgccgtcatg ccgcttcgcc ctggaccagc tggaggcgcg
     2401 ccggatccga ccatccgtgc agctcggcca cgatgtccgc ggtggtcctc tcggcgatgg
     2461 ccggcaccag ctcccggcac cgggcgccgc acgcggtccg gccgcggtgg agcagctcca
     2521 tgagatggga ggggagaggg gcgccggccg cggccgtcag ccggcgcacg agcgcccact
     2581 ccggcccgag caggcccagg ggcaggcgcc ggcccatgag ggcggcctgg gagacggtga
     2641 ccgcgccggg ggcgaccacg ttgagcgggc cgtcgatgcc ggcccgaaga gcggccacca
     2701 ccgcggccgc ggcatcgtcc gcgtgcacca ccgagaacgg tgggtcggcc aacgcgctga
     2761 acggcaccac cggcagccgc agcagtcggc cgagcggcga ggggaaccgg ggcccgacca
     2821 ggggggcgaa gcggagggcg gccaccggca cgcccccgtc ctggccggtg gcgaaggcca
     2881 ggcgctcgac ctcgagcagc gaccggccga acgcggaggt cggcaccggc ggcaccgcct
     2941 cgtcgggcac ggtgaccgat cccctccccc ggccgtagac ctccagcccg ctgcgcagca
     3001 cgacccgctc cagggccccg aggtcggcgg cctcgccgag cacggccagg gtggccgact
     3061 cggtccgctc ccacgcctcg tgcggggtgg agcgggcgga cggctcgtag atgccgagat
     3121 gcacgatggc cgtgggctcc acctcccgca ccaggcgcag caggggccgg cggtcgcggg
     3181 gatcgatgag ccggaaggtc atgttccgca cccgaccccg gggcgggtac atgtcggtcc
     3241 ccaccaggtc gctgacgccc gggtcggcgg ccaggagcag ggccaccttg cggcccaggt
     3301 cgccgcccac gccggtgacc aggaccctca cgaacccgct tcgggccgca gggtcgggaa
     3361 gaggatgacg tcgcggatgg ccgccacgtc ggccagcagc atgaccagcc gg
//
LOCUS       DASWGG010000188         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2024202, whole genome shotgun sequence.
ACCESSION   DASWGG010000188 DASWGG010000000
VERSION     DASWGG010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024202"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VF954_03425"
     CDS             complement(<1..758)
                     /locus_tag="VF954_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9530163.1"
                     /translation="MFTVTFKGLWAHRRRLVGMLAAVVLGVGFLAGALALGDTLSANF
                     NNLFASANAGTDALVRSSTSFGSGVEAVRPTVPLSLVDTVRGVPGVAAAEPSITGFGE
                     IIGSDGTAVGGNGPPRLATYWIPDAALNPYRLAAGRAPASPDEVVINRGAATAGRLHV
                     DDTTTVLMPTPVKVHIVGIATFAGADGLGTATTAAFDLQGAERYIVGSAGGPATGSTA
                     GPNQETGQASNIVVRARSGVSQAEIATRIQKVLPP"
     gene            complement(798..1535)
                     /locus_tag="VF954_03430"
     CDS             complement(798..1535)
                     /locus_tag="VF954_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9530164.1"
                     /translation="MQTLTMAAARAVDATKVYGSADTAVRALDEVSVGFPQGCFTAIM
                     GPSGSGKSTLMHCLAGLDQLSSGKVFLGDVELGSLSDTELTLLRRERVGFIFQSFNLL
                     PTLTAAENITLPLLLAGKRPDRDWLDTVIAEVRLGDRLSHRPSELSGGQQQRVAVARA
                     LVTRPEIIFADEPTGNLDSRSSAELLGFLRRAVQSFDQTIVMVTHDPAAAAAADGVLF
                     LSDGRIVGELREPTAETVLDQLKAIGE"
BASE COUNT          222 a          535 c          601 g          243 t
ORIGIN      
        1 ggcggcagca ccttctggat ccgggtggcg atctcggcct ggctcacgcc ggaccgtgcc
       61 cgcacgacga tgttcgacgc ctggcctgtt tcctggttgg gccccgccgt ggacccggtg
      121 gcgggcccgc cggcggaccc gacgatgtag cgctcggcgc cctggaggtc gaaggcagcc
      181 gtggtggccg tcccgaggcc gtcggcaccg gcaaacgttg cgatgccaac gatgtgcacc
      241 ttcacggggg tgggcatgag caccgtcgtg gtgtcgtcga cgtgcagcct gcccgcggtg
      301 gccgccccgc ggttgatcac gacctcgtcg ggcgaggccg gcgccctgcc cgctgcgagc
      361 cggtaggggt tgagggcggc gtcggggatc cagtaggtgg cgagccgcgg cggcccgttg
      421 cccccgaccg cggtcccgtc cgaaccgatg atctccccga accccgtgat cgacggctcc
      481 gctgcggcga ccccgggcac gccgcggacc gtgtcgacca gcgacagcgg gacggtgggc
      541 cggaccgcct ccacgccgct cccgaaactg gtggagctgc ggaccagggc gtcggtcccg
      601 gcgttggccg aggcgaagag gttgttgaag ttggcgctga gggtgtcgcc gagtgcgagg
      661 gcgccggcga ggaacccgac gccgagcacg acggcggcca gcatcccgac gaggcgccgg
      721 cggtgtgccc acagcccttt gaacgtgacg gtgaacatgg tgatcgtccc ccctttcgtt
      781 ggtagctcct gccgggtcta ctcgccgatg gccttgagct ggtcgaggac cgtctcggcg
      841 gtcggttccc gcagttcgcc gacgatccgc ccgtcggaaa ggaacagcac gccgtcggcc
      901 gccgccgcgg cggcagggtc gtgggtcacc atcacgatcg tctggtcgaa ggactgcacc
      961 gcccgccgga ggaagccgag cagctcggcg ctggagcggg agtcgaggtt gcccgtgggc
     1021 tcgtcggcga agatgatctc cgggcgggtc accagggccc gggccacggc gacccgctgc
     1081 tgctggcccc cggagagctc ggacgggcgg tgcgagaggc ggtccccgag ccgcacctcc
     1141 gcaatcacgg tgtcgagcca atcccggtcg ggccgcttgc ccgcaagcag cagcggcagg
     1201 gtgatgttct ccgcagcggt cagcgtgggc agcaggttga aggactggaa gatgaagccg
     1261 acccgctccc ggcgcaacag cgtcagctcg gtgtcggaga gcgatcccag ctcgacgtcg
     1321 ccgagaaaga ccttgccgga ggagagctgg tcaaggccgg ccaggcagtg catcagtgtg
     1381 gacttgccgg accccgacgg ccccatgatg gcggtgaagc atccctgggg aaaccccacg
     1441 gacacctcgt cgagggcgcg cacggcggtg tcggccgagc cgtagacctt ggtggcgtcg
     1501 acggctcgtg ccgctgccat ggtgagggtt tgcatggaac ctcctccttc atcgttggcg
     1561 tcgtcgttgg cgtcgtcgtt ggcttcgttg gtggcctggt c
//
LOCUS       DASWGG010000189         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1160026, whole genome shotgun sequence.
ACCESSION   DASWGG010000189 DASWGG010000000
VERSION     DASWGG010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160026"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VF954_03435"
     CDS             complement(<1..944)
                     /locus_tag="VF954_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9530165.1"
                     /translation="MATRELDFAERFEAVVDNVELVIQGKDDAVRLALVCMVAEGHLL
                     IEDVPGVGKTSLAKALARSLSGDWNRIQFTPDLLPSDVTGVTVFSRSTETFEFRPGGV
                     FANIVLGDEINRASPKTQSALLESMEERQVTVDATTYRLPSPFMVIATQNPIDHEGTY
                     PLPESQLDRFLMRITMGYPDRDAEISMLEAHATASVIDEIQAVADPEDIDAMADVARY
                     THVAPALRAYIVDLASASRSHPSVALGMSPRAALALQRAGRALAAARGRQYVIPDDLK
                     FLARPVLEHRLMLTPEAQLGGATVADVIDEVLNLVPVPT"
     gene            1053..>1621
                     /locus_tag="VF954_03440"
     CDS             1053..>1621
                     /locus_tag="VF954_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX9530166.1"
                     /translation="MDHHEWHEELALAVDIGGTKLAAGLVDGAGHLSGAVRAPTPGRD
                     GAAIWRVLDELVRRVVAGAPTPPLVCGVGCGGPMELGGETVSPLNIAAWRRFPLRPRL
                     GALTGLPTFVDNDAKALALAEGWVGAAAGRPAFLAMVVSTGVGGGIVLDGRLLDGRLG
                     NAGHVGHVIVEPDGRACRCGGRGCLEAEAS"
BASE COUNT          196 a          555 c          634 g          236 t
ORIGIN      
        1 gtcggcaccg gcaccaggtt gaggacctcg tcgatgacgt cggcgacggt ggctccgccc
       61 agctgggcct ccggcgtcag catcaggcgg tgctcgagca ccggccgggc caggaacttc
      121 aggtcgtcgg ggatcacgta ctggcggccc cgggccgcgg cgagggcccg gccggcgcgc
      181 tgaagggcca gggcggcccg gggtgacatg cccagggcga cgctcgggtg gctgcggctg
      241 gcggaggcca ggtccacgat gtaggcgcgc agggccgggg ccacatgggt gtagcgggcg
      301 acgtcagcca tggcgtcgat gtcctcgggg tcggccaccg cctggatctc gtcgatcacg
      361 ctcgccgtgg cgtgcgcctc gagcatgctg atctcggcgt cgcggtcggg gtaccccatg
      421 gtgatgcgca tcaggaaccg gtcgagctgg ctctccggta gcgggtaggt gccctcgtgg
      481 tcgatcgggt tctgggtggc gatcaccatg aacggcgagg gcagccggta ggtcgtggcg
      541 tccacggtga cctggcgctc ctccatggac tcgagcaggg cggactgcgt cttcggcgag
      601 gcccggttga tctcgtcgcc gagcacgatg ttggcgaaca cgccgccggg gcggaactcg
      661 aaggtctccg tcgaccggct gaacaccgtg accccggtca cgtccgaggg gagcaggtcg
      721 ggggtgaact ggatgcggtt ccagtccccg gagagggacc gggccagcgc cttggccagg
      781 ctcgtcttgc cgacccccgg aacgtcctcg atcaggagat ggccctcggc gaccatgcag
      841 accagcgcca gccggacggc gtcgtccttt ccctggatca cgagctcgac gttgtctacg
      901 acggcctcga agcgctcggc gaagtcgagc tcgcgtgtcg ccatcgatcg ctgcctgggc
      961 aagcgttgcc tcctgggacg gttcgggcgc aagggtacgg gccgggtggt cggggagcca
     1021 gccccagccc cgccgcccgt agcctggccc gggtggacca ccacgaatgg cacgaggagc
     1081 tcgccctggc cgtcgacatc ggcgggacga agctggcggc tgggttggtc gacggcgcgg
     1141 gccacctctc cggcgccgtg cgggcaccca cccccgggcg cgacggcgcc gccatctggc
     1201 gcgtgctcga cgagctggtc cgtcgcgtag tggccggcgc gcccacgccg cccctcgtgt
     1261 gcggcgtcgg ctgcggcggg cccatggagc tggggggcga gaccgtctcg ccgctcaaca
     1321 tcgcggcgtg gcgccggttc ccgctgcgcc cccgcctggg cgccctgacc ggcttgccga
     1381 ccttcgtgga caacgacgcc aaggcgctgg ccctggccga ggggtgggtg ggtgccgcgg
     1441 ccgggcggcc cgccttcctg gccatggtcg tctccacggg cgtgggcggc ggcatcgtgc
     1501 tcgacgggcg cctcctcgac gggcggctcg gcaacgccgg ccacgtgggc catgtgatcg
     1561 tcgaacccga tggccgggcc tgccggtgcg ggggccgcgg gtgcctggag gccgaggcgt
     1621 c
//
LOCUS       DASWGG010000190         6976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3089165, whole genome shotgun sequence.
ACCESSION   DASWGG010000190 DASWGG010000000
VERSION     DASWGG010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3089165"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VF954_03445"
     CDS             complement(<1..260)
                     /locus_tag="VF954_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX9530167.1"
                     /translation="MIGGLRREPSAVAGTCLGVALVAIGTAVLKAAASGTTRGTPAVI
                     LVLPVVLAGIIGGRGAALLTAAAAAAALNLAFIQPLWTFKIN"
     gene            381..2744
                     /locus_tag="VF954_03450"
     CDS             381..2744
                     /locus_tag="VF954_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9530168.1"
                     /translation="MKTEPELTGVSDLPANPPEPLQPTAPAAAGASIEVPETLGYRLK
                     TRLLGQPLYTEDLVHERLGKPTALAVFASDNLSSSAYATEEILRVLVPVVGVAAFSLV
                     VPVTTALLVVLGFLILSYRQTIKEYPSAGGAYIVTKDNFGKVPALVAGVSLLTDYVLT
                     VSVSVAAGTAALASAFSPLTSWVVPISVAFVVLIAYGNLRGVKESGKVFAAPTYFFLG
                     NMVLLLGWGISRLAFGHLPRLATHGSGLVKVGTPGNGLFMGAALFVVLRSFASGGAAV
                     TGVEAISNGVPAFREPAWRNARTTLVWMGSLLGAMFFGLSVLAGAMHVTPFEKGTPTV
                     IAQVGKYVYGSTAFGHLLFFSLQAGTMLILVLAANTSFADFPRLASFAAADSFLPRQL
                     QKRGHRLVFSNGIVFLAGAAIVLVIATEAKVSRLIPLYAIGVFTSFTLSQAGMAKHHI
                     RKREPGWQRGLFINGMGAVLSLVVDIIIAVTKFAEGGWVVIAAVPLLVAVLLRLNRQY
                     REEGDELREDAPKAAAAPILSRHVVLVLVDQMDLAAARAIQYARTLTPDDLRAVHFAV
                     DPVRAHDLRTAWTDLGLARVALDIIECPDRRLTRAAVDLAAGLADGETEVTVLLPRIE
                     HSRGWHRLLHDRTSNTIARALSDLPHTNVTFVPYHLGRVHPPSSGRSGPPRHERRGPA
                     HPVPPVMPAGVSVPSGTVPIGTAAARQRVTVAGRVKAVRVQPRAGVATVECTISDGTG
                     EIVVVFLGRRHVGGWEPGGFVAVTGVVAEHGGRREMLNPDYELLVAS"
     gene            2744..3046
                     /locus_tag="VF954_03455"
     CDS             2744..3046
                     /locus_tag="VF954_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530169.1"
                     /translation="MPSTRRSRPALDRLLAFDEDAAEAVAVMTLLSSGRPEDQALGAS
                     LLADLLARPNGAERAGRGLASVAGGLLALLEFYGGVPPAEALRELGRLVAEAVAAE"
     gene            complement(3086..5380)
                     /locus_tag="VF954_03460"
     CDS             complement(3086..5380)
                     /locus_tag="VF954_03460"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF002895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX9530170.1"
                     /translation="MSLERGPLLANDKLTGAPWCTAHAQLVDAWLARLVTDAAQGRLD
                     GLALVAVGGYGRAELCPQSDIDVYLIHAGRSDAAAVAERVWYPVWDESLHLGHSVCTV
                     RQALGLASDDVELATALLSARHIAGDDNLTAALAKGGLQLWERKSRRWLGELGARVAA
                     RHDKAGEVAFLLEPDLKEGRGGLRDAHALAWAEAAHRIMLDHDETALSQAYSVLLGAR
                     VELQRHTRRSSNVLTLEAQAAVAHALGLADEDALMLRVAEAARTIAWTSDDTWRRIRS
                     ALRGPLGRVARRNRVVDADIVMRDGEISVTESAVFGGPTLVLRAAAAAAANHTVIERG
                     SLERLAARRPAEGDTWPADARAALIALLLSGRAAITVVEALDQRGIWGALLPEWRHVR
                     ARPQRNPYHRYTVDRHLLETVANAAKMAGRVDRADLLVVAALLHDIGKGLAGDHTHHG
                     MALARGVAVRIGLAPEDVETIVALVEHHLLLPEVATRRDLDDPTTVQRVADRVGSPQL
                     LDLLAALAEADGLATGPAAWGPWKAELVARLVNRVEDRMRGVEGGTLAGLFPTEDQLA
                     RLAGLAGGRSCVEADGSILTVMAVDRPGVFSRIAGVVALHGLDVVAAAAHSTEGGRAL
                     AVFTVVDPFRDAIPWDRVKADVHRALDGRLAVSARLAERARTYRRRGPATRPAATQVH
                     FDIDASTAATVIDVHTTDGIGVLYRITRALAELDLDIRSARVHTLGGHVVDAFYVRDA
                     IGAKITDPVILAEIERAVVHGLDT"
     gene            5493..>6976
                     /gene="amt"
                     /locus_tag="VF954_03465"
     CDS             5493..>6976
                     /gene="amt"
                     /locus_tag="VF954_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX9530171.1"
                     /translation="MIRKRLQRLKDKDTRRLIALIVGGKMLGIVALLGVIKGAAWYFD
                     TAATAQAAQTVAHQANDFVSPINTVWTLVTAFLVFFMQAGFMGLEAGFARSRETVNVL
                     LECVFDTCLCGLLYWAFGFAFQFGNGNGIIGHQYFLLHGMTPAYGTTQVAFLAFFLFQ
                     FAFADTASTVTSGAMVGRTGFKGDILYSTCVSGFIYPIFGHWVWGPGGWLGNTMGWFG
                     HGGLALTGNAVFRDFAGSTVVHTVGGFIALGGAIALGPRLGRTFKRDGGGPLPPHDLT
                     IGAIGGVILWFGWYGFNPGSTLSAMDWTGIGRVAANTTLAACAAGMSAVILIYPRTKK
                     WDLGISVNGFLGGLVAITCPCYWVSPFGAVVIGAVAGGVVIIGIDLLEYLRIDDPIGA
                     WPVHGLAGIWGTLSLGLLATGQYGIPTADGFDTTTTVRGLFYGGGGEQLFAQFIGSMT
                     CVVCVLGVTLLIMYAIKSIRGTWTLRISQEGELEGMDIHEHGTPA"
BASE COUNT          942 a         2512 c         2442 g         1080 t
ORIGIN      
        1 ctgttgatct tgaaggtcca gaggggctgg atgaatgcca ggttcaaggc cgccgccgcg
       61 gccgccgcgg tgagcagggc cgcgccgcga ccgccgatga tcccggcgag cacgacgggc
      121 agaacgagga tcacagctgg agtccccctg gtggtgcccg acgcggccgc cttcagcacg
      181 gcggtgccga tcgccaccag cgccacgccg agacacgtgc ccgcgaccgc gctgggctcg
      241 cggcgcaggc ctccgatcat gggctgattg tcgccgatcc ggggcggatg tgcggggcac
      301 ccacccagca ccgcctgtat gaagtattta tgctgtcggg agcatcgcaa gagcccgacc
      361 tgcgaagggg acgccacgta gtgaagaccg aaccggagct gaccggggtc tcggacctcc
      421 cagcgaaccc gcccgaaccc ctccagccca cggcacccgc cgcagccggc gcgagcatcg
      481 aggtgccgga gaccctcggc taccggctca agacccggct cctcggccag ccgctctaca
      541 ccgaggacct ggtgcacgag cggctgggca agcccacggc gctggccgtc ttcgcgtccg
      601 acaacctgtc ctcctcggcg tacgccaccg aggagatcct gcgggtgctg gtgccggtcg
      661 tgggtgtggc ggccttctca ctcgtcgtcc cggtcacgac cgcgcttctc gtcgtgctcg
      721 gcttcctgat cctctcgtac cgccagacca tcaaggagta ccccagcgcg ggcggcgcct
      781 acatcgtcac caaggacaac ttcggcaagg tcccggccct ggtcgcgggc gtgtcgctgt
      841 tgaccgacta cgtcctcacg gtctcggtct cggtcgcggc cggtaccgcg gccctggctt
      901 cggccttcag cccgctgacc tcgtgggtgg tgcccatctc ggtcgccttc gtggtgctga
      961 tcgcctacgg gaacctgcgg ggggtcaagg agtcgggcaa ggtcttcgcc gcccccacct
     1021 acttcttcct gggcaacatg gtcctgctgc tcggatgggg gatctcccgg ctggcgttcg
     1081 ggcacctccc gcggctggcg acgcacggca gcggcctggt gaaggtcggc acgcccggca
     1141 acggcctgtt catgggcgcg gcgctcttcg tcgtgctgcg gtccttcgcg tccggcgggg
     1201 cggccgtcac gggcgtcgag gccatctcca acggcgtgcc cgcgttccgt gagccggcgt
     1261 ggcgcaacgc ccggaccacc ctggtctgga tgggatcgct gttgggcgca atgttcttcg
     1321 ggctgtccgt gctggccggc gccatgcacg tcacaccctt cgagaagggc acgccgacgg
     1381 tcatcgccca ggtgggcaag tacgtgtacg gatcgacggc gttcggccac ctgctcttct
     1441 tctccttgca ggccgggacc atgttgatcc tcgtgctggc ggccaacacc agcttcgccg
     1501 acttcccccg cctggccagc ttcgcagctg ccgacagctt cctgcccagg cagctccaga
     1561 agcgcggcca ccgcctggtg ttctccaacg gcatcgtgtt cctggccggg gccgccatcg
     1621 tgctggtcat cgccaccgag gccaaggtca gccgcctcat cccgctctac gccattggcg
     1681 tgttcaccag cttcaccttg tcccaggccg gcatggccaa gcaccacatc aggaagcggg
     1741 agccgggctg gcagcgaggg cttttcatca acgggatggg cgcggtcctg tccctggtgg
     1801 tcgacatcat catcgccgtc accaagttcg ccgagggtgg atgggtggtc atagccgccg
     1861 tccccctcct cgtagccgtg ctgctgcgcc tcaaccggca gtaccgggag gagggcgacg
     1921 agctgaggga ggacgccccg aaggcagccg cggccccgat cctgagccgc cacgtggtcc
     1981 tggtcctggt ggaccagatg gacctggccg cggcccgcgc catccagtac gcccggacct
     2041 tgacgccgga cgacctgcgc gccgtgcatt tcgccgtcga tccggtgcgg gcccacgacc
     2101 tgaggacggc gtggacggac ctcggcctcg cccgcgtggc cctggacatc atcgagtgcc
     2161 cggaccgccg gttgacccgg gccgcggtgg acctggccgc cggcctcgcc gacggcgaga
     2221 ccgaggtgac cgtgctgctg ccccgcatag agcacagccg cggctggcac aggctgctcc
     2281 acgaccggac ctccaacacg atcgcgcgcg ccctgtccga cctcccccac accaacgtga
     2341 ccttcgtccc gtaccacctg ggccgcgtcc accccccctc atcgggacgg tccggcccgc
     2401 cccggcacga gcgccgcggt ccggcccatc cggtcccgcc ggtgatgcct gccggggtca
     2461 gcgtgccttc cggcacggtg cccatcggca ccgccgcggc ccggcaacgg gtgaccgtcg
     2521 ccggtcgcgt caaggcggtg cgggtgcagc cccgggccgg tgtggcgacg gtcgagtgca
     2581 ccatctccga cggcacgggg gagatcgtcg tggtgttcct gggccggcgc cacgtcggcg
     2641 gatgggagcc gggagggttc gtcgcggtca ccggggtggt ggccgagcac ggcggccgca
     2701 gggagatgct caatcccgac tacgagctcc ttgtcgccag ctgatgccct ccacccgccg
     2761 cagccggccg gcgctcgatc ggctgctggc cttcgacgag gatgcggccg aggcggtcgc
     2821 cgtgatgacg ttgctctcga gcggtcggcc cgaggaccag gcgctggggg cgtccctgct
     2881 cgccgacctg ctcgcccgcc cgaacggggc cgagcgggcg gggcggggcc tggcgtcggt
     2941 cgccggcggc ctgctcgccc ttctcgagtt ctacggcggt gtgcccccgg cggaggcgct
     3001 ccgcgagctg ggccggctcg tggcggaggc tgtcgccgcg gagtgatcct ggggaaagcc
     3061 ccctcggtgc ccgacacgct ccgcgctagg tgtccaggcc gtggacgacg gcgcgctcga
     3121 tctcggccag gatcacgggg tcggtgatct tggcgccgat cgcgtcccga acgtagaacg
     3181 catcgacgac gtggccgccc agggtgtgca cccgggcgga gcggatgtcg agatccagct
     3241 ccgccagggc ccgggtgatg cggtagagca cgccgatgcc gtcggtcgtg tgcacgtcga
     3301 tcacggtggc cgcggtggag gcgtcgatgt cgaagtgcac ctgtgtagcg gccggccgcg
     3361 tcgccggtcc tcgccggcgg taggtcctgg ctcgctcggc cagccgggcg ctcaccgcga
     3421 ggcgcccgtc tagggcccgg tggacgtcgg ccttgacccg gtcccacggg atggcgtccc
     3481 ggaacgggtc gaccacggtg aacaccgcca gggcccgtcc tccctccgtc gagtgggctg
     3541 ccgcggcgac cacgtccagg ccgtgaaggg ccacgacccc tgcgatccgg ctgaacacgc
     3601 ctggccggtc gacggccatc accgtgagga tgctgccgtc cgcctcgacg cagctgcggc
     3661 ccccggccag cccggccagc cgggccagct ggtcctcggt cgggaagagg ccggccaggg
     3721 tcccgccttc cacgccccgc atgcggtcct ccacccggtt gacgagtcgg gcgaccagct
     3781 cggccttcca cggtccccag gcggccggtc cggtcgcgag gccgtccgcc tcggccaggg
     3841 cggccagcag gtcgaggagc tgcggcgatc ccacccggtc ggcgacgcgt tggacggtgg
     3901 tcgggtcgtc gagatcgcgc cgggtggcca cctcggggag cagcaggtgg tgctcgacca
     3961 gcgccacgat cgtctcgacg tcctcgggag ccagaccgat gcgcaccgcc acaccacggg
     4021 cgagggccat gccgtggtgg gtgtggtccc cggccaagcc cttgccgatg tcgtgcagga
     4081 gggccgcgac gaccaacagg tccgcccgat cgacccgtcc ggccatcttg gccgcgttgg
     4141 ccaccgtctc gagcaggtgc cggtccaccg tgtagcggtg gtaggggttg cgttggggcc
     4201 gggctcgcac gtgccgccac tcgggcagca atgcgcccca gatgccgcgc tggtcgaggg
     4261 cctccaccac ggtgatcgcc gcccgtccgg acagcagcag ggcgatgagc gccgcccggg
     4321 cgtctgcggg ccacgtgtcg ccttcggccg gtcgccgggc ggccaggcgc tctagcgaac
     4381 cgcgctcgat gaccgtgtga ttggcggccg cggccgcggc ggcgcgcagc acgagcgtgg
     4441 gcccgccgaa caccgccgac tcggtgacgg agatctcacc gtcgcgcatg acgatgtcgg
     4501 catccaccac gcggttgcgc cgggccaccc ggccgagcgg cccccgcagc gcggagcgga
     4561 tgcgccgcca ggtgtcgtca ctggtccacg cgatggtgcg ggccgcctct gcgacccgga
     4621 gcatcagggc gtcctcgtct gccaggccga gtgcgtgggc cacggcagcc tgggcctcta
     4681 gggtgagcac attggacgac cggcgggtat ggcgttgcag ctccacccgg gcgccgagca
     4741 gaacggagta ggcctgggac agggcggtct cgtcgtggtc gagcatgatc cgatgggccg
     4801 cctccgccca ggccagggcg tgggcgtcgc gcagcccgcc ccggccctct ttcagatcgg
     4861 gctcgagcag gaaggcgacc tcgccggcct tgtcgtggcg agcggcgacc cgcgccccca
     4921 gctcgcccag ccagcgccgg gacttgcgct cccagagctg gaggccgccc ttggcgagcg
     4981 ccgcggtcag gttgtcgtct ccggcgatgt ggcgggcgct caacagcgcg gtagcgagct
     5041 cgacgtcgtc ggacgccagt cccaacgcct ggcggacggt gcacacgctg tggccgaggt
     5101 ggagcgactc gtcccagacg gggtaccaca cccgttccgc gacggcggcg gcgtcgctgc
     5161 gcccggcatg tattaggtag acgtcgatgt cgctctgagg gcacagctcg gcccgcccgt
     5221 agcctcccac cgccaccagc gccaggccat cgaggcgccc ctgggcggca tcggtcacca
     5281 gacgagcgag ccaggcgtct accagctggg cgtgggcggt gcaccacggg gcgcccgtca
     5341 gcttgtcgtt ggccagcaac ggcccgcgct ccaggctcat cacgggcatc gtaggagccc
     5401 ggccccagat gagcggcacc tggataaaat tttcataaat agaaacattg tcgtgacaac
     5461 cccgaaatgt gggctgcata cggtctcggc tcatgatccg aaaacgactg cagcggctca
     5521 aggacaagga caccaggcgg ctcatcgccc tgatcgtggg gggcaagatg ctcggcatcg
     5581 tcgccctcct cggagtgatc aagggggcgg cctggtactt cgacaccgcg gccaccgcgc
     5641 aggcggcgca gaccgtggcc caccaggcca acgacttcgt gagcccgatc aacaccgtgt
     5701 ggaccttggt cacggcgttc ctggtcttct tcatgcaggc cgggttcatg ggcctcgagg
     5761 ccggcttcgc ccggtcccgt gagacggtca acgtgctcct cgagtgcgtc ttcgacacgt
     5821 gcctgtgcgg gctcctgtac tgggccttcg gcttcgcctt ccagttcggc aacggcaacg
     5881 gcatcatcgg ccaccagtac ttcctgctcc acggcatgac cccggcgtac ggcacgacgc
     5941 aggtggcctt cctggccttc ttcctgttcc agttcgcctt tgccgacacc gcgtcgaccg
     6001 tcacctcggg tgccatggtc gggcgcaccg gtttcaaggg cgacatcctc tacagcacct
     6061 gcgtgtcggg cttcatctac ccgatcttcg ggcactgggt gtgggggccc gggggctggc
     6121 tgggcaacac catgggctgg ttcggccacg gtggcctcgc cctcaccggc aacgcggtgt
     6181 tccgcgactt cgccggctcg acggtggtcc acaccgtcgg tgggttcatc gccctcggag
     6241 gcgccatcgc cctgggccct cgcctcgggc gcacgttcaa gcgagacggc ggcggcccgc
     6301 tgccgcccca cgacctgacg atcggcgcca tcggcggcgt catcctgtgg ttcggctggt
     6361 acggcttcaa ccccggctcg accctgtccg ccatggactg gacgggcatc ggccgtgtcg
     6421 ccgccaacac aaccctcgcc gcctgcgcgg ccgggatgtc ggcggtgatc ctgatctatc
     6481 cgcggaccaa gaagtgggac ctcggcatct ccgtgaacgg cttcctgggc ggcctggtgg
     6541 ccatcacctg cccgtgctac tgggtctcgc cgttcggagc ggtcgtgatc ggcgcggtgg
     6601 cgggcggggt ggtcatcatc ggcatcgacc tgctggagta cctgcgcatc gacgacccca
     6661 tcggcgcctg gcccgtgcac ggcctggccg gcatctgggg cacgctgagc ctggggctgc
     6721 tggcgacggg ccagtacggc ataccgacgg ccgacgggtt cgacaccacg accacggtgc
     6781 ggggcctgtt ctacggaggt ggaggcgagc agctcttcgc ccagttcatc ggcagcatga
     6841 cctgcgtcgt atgcgtcctc ggagtcacgc tgctgatcat gtacgccatc aagtccatcc
     6901 ggggaacctg gacgctgcgg atatcccagg agggcgagct cgaggggatg gacatccacg
     6961 agcacggcac gcccgc
//
LOCUS       DASWGG010000191         2929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2346187, whole genome shotgun sequence.
ACCESSION   DASWGG010000191 DASWGG010000000
VERSION     DASWGG010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2346187"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1370)
                     /locus_tag="VF954_03470"
     CDS             complement(6..1370)
                     /locus_tag="VF954_03470"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9530172.1"
                     /translation="MSPTRLAPVAAPRPVPGVAHTPPPRVPTVGDRTLANGLRVIAVR
                     RPGVPRFEIRLRVPLVRGSVADAGRTRLLPEALLSGTAERSSLEIARRLEELGADLNA
                     ASDVETLYVNGSALTSTAGDFFDLLADVVLAPEFPTAEVAIERERVAHELTITQSQPA
                     AVASAAAARRLYGSHPYGRGFTPPDAVRKIGRAGLVRAHADRVRPAGATLVVVGDLRP
                     ERAAAEAERVFGAWSPGAPVTGAPAPPPFSTGPVLLVDRPGAVQTNIRMVGPAVSRRD
                     PWYAALSAALTAFGGYFSSRLTINIRERRGFTYSPGARMDHWQSSSHLFVAADVATEV
                     TAPALAEIDYELARMAASPLDEEELVAVQRYLVGNLLTAVQTQAGLATYLAVLVGQGL
                     GITYLRDQPRLIERVTLASAHEAGARFLGPPRLATILVGDAARILDRVSSLADVVVES
                     LPGV"
     gene            complement(1375..2721)
                     /locus_tag="VF954_03475"
     CDS             complement(1375..2721)
                     /locus_tag="VF954_03475"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9530173.1"
                     /translation="MPSSRSEFTVERFELANGLRVVVVPDRSSPSATVAVYYDVGFRS
                     EPEGRTGFAHLFEHLMFEGSASLPKLAHAKMVQGNGGNFNGSTSFDYTNYFEQLPSGA
                     LELGLFLEADRMRAPRLDLETLHNQIAVVKEEIRVNVLNRPYGGFPWIELPPVLFRSF
                     NNAHNGYGSFVDLESVTVEDAVDFWTRYYAPGNAVLSITGDVTTEAAHRLVERHFGDV
                     PGRKVARPSDTSEPVPDQERRGVHHDPMAPTPALAVAWRVPDPLDTPRYAAMVMAAQV
                     LGGGDASRLHQRLVKKDQLATVVGTSVSAFGDPFEVRDPTFGQLICYYQDAPVERLLT
                     VFDEECARLAHDGLEPGELQRSVNAWASGYYRHLDNQVQRGLTAAVLEQQRGRAELLN
                     ELPSLLGAVTEDDVVSAVGEWLSPSRRAVLEWRAGPAPAPAPRGARARRRPSAGRG"
     gene            2747..>2929
                     /locus_tag="VF954_03480"
     CDS             2747..>2929
                     /locus_tag="VF954_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530174.1"
                     /translation="MLSELVGFTNLSPTTTQEVVMAIQLGDTAPDFTADTTEGTLTFH
                     EYLGDGWGILFSHPKDF"
BASE COUNT          429 a         1034 c         1094 g          372 t
ORIGIN      
        1 cgcgctcaga cgcccggaag gctctccacc accacgtccg cgagtgagct cacccggtcc
       61 aggatgcgcg ccgcgtcgcc gacgagaatg gtcgccagcc ggggcgggcc caggaagcgg
      121 gccccggcct cgtgggcgga ggccagggtc acccgctcga tcagccgggg ctggtcccgc
      181 agataggtga tgccgagacc ctgtccgacc agcacggcga gataggtcgc cagcccggcc
      241 tgggtctgca cggccgtcag caggttgccc accaggtagc gctggacggc gaccagctcc
      301 tcttcgtcga gtggggaggc ggccatgcgg gccagctcgt agtcgatctc ggccagggcc
      361 ggggcggtca cctccgtcgc cacgtcggcg gccacgaaca ggtgcgagga gctctgccag
      421 tggtccatcc gggccccggg cgagtaggtg aacccccgcc gctcgcggat gttgatggtc
      481 aggcgtgagg agaagtaccc gccgaaggcc gtcagggccg cgctcagcgc ggcataccac
      541 gggtcccgcc gggagacggc cgggccgacc atccggatgt tggtctgcac cgcgccggga
      601 cggtcgacga gcaggaccgg cccggtcgag aagggaggcg gggcgggcgc gccggtcacg
      661 ggcgcccccg ggctccacgc tccgaacacc cgctcggcct cggcggcggc ccgttcgggg
      721 cgcagatcgc cgacgaccac gagcgtggcc cccgccgggc ggacccggtc ggcgtgggct
      781 cgcacgagac cggcccggcc gatcttgcgg accgcatccg gcggtgtgaa gccccggccg
      841 taggggtggc tgccgtacag ccggcgggcc gccgccgccg acgccactgc ggccggctgg
      901 ctctgggtga tggtcagctc gtgggccacc cgctcgcgct cgatggccac ctcggcggtc
      961 gggaactcgg gggccagcac cacgtcggcc agaaggtcga agaagtcccc agccgtcgag
     1021 gtcaacgccg agccgttgac gtacaaggtc tcgacgtccg acgccgcgtt caggtcggct
     1081 ccgagctcct cgagccggcg ggcgatctcc aggctcgacc gctcggccgt gccggagagg
     1141 agtgcctccg gcagcaaccg ggtgcgcccg gcatcggcga ccgagccgcg gaccagcggg
     1201 actcgcaggc gaatctcgaa tcggggcacg cccggccggc gcacggcgat gacacgcagg
     1261 ccgttggcga gggtgcggtc tccgacggtc ggcacccggg gcggcggggt gtgggccacc
     1321 ccggggaccg gtcgcggggc ggcgacgggt gcgaggcgcg tcgggctcac cggctcaccc
     1381 ccgtcccgcg ctcggccggc ggcgggcccg ggcaccccgg ggcgccggcg ccggcgcggg
     1441 gccggcccgc cactcgagca cggcgcgccg ggaggggctc agccactcgc ccaccgcgga
     1501 cacgacgtcg tcttcggtga cggcgccgag caggctgggc agctcgttga gcagctcggc
     1561 ccgcccccgc tgctgctcga gcacggcggc ggtgaggccc cgctgcacct ggttgtccag
     1621 gtggcggtag tacccggagg cccaggcgtt gaccgagcgc tggagctcgc cgggctccag
     1681 cccgtcgtgc gccaggcggg cgcactcctc gtcgaagacc gtcagcagcc gctccaccgg
     1741 ggcgtcctgg tagtagcaga tgagctgccc gaaggtcggg tcccggacct cgaagggatc
     1801 cccgaaagcg ctcaccgagg tgccgacgac cgtggccagc tggtccttct tcaccagccg
     1861 ttggtgcagc cgcgaggcgt cgccgccacc gaggacctgc gccgccatca ccatggcggc
     1921 gtaccgcggg gtgtcgaggg gatcgggcac gcgccacgcc acggcgagcg ccggcgtggg
     1981 cgccatcggg tcgtggtgca cgccgcggcg ttcctggtcg gggacgggct cggaggtgtc
     2041 cgaggggcgg gccaccttgc ggcccgggac atcgccgaaa tgccgctcga cgagacggtg
     2101 cgcagcctcc gtcgtgacgt cgccggtgat cgagagcacg gcgttgcccg gcgcgtagta
     2161 gcgggtccag aagtcgaccg cgtcctcgac ggtgaccgac tcgaggtcga cgaaggaccc
     2221 gtagccgttg tgggcgttat tgaaggaccg gaacagcacg ggtggcagct cgatccaagg
     2281 gaacccgccg tagggccggt tgaggacgtt gacccggatc tcctccttca ccaccgcgat
     2341 ctggttgtgc agcgtctcca ggtcgaggcg cggggcgcgc atccggtcgg cctcgaggaa
     2401 cagcccgagc tcgagcgcac cggacggcag ctgctcgaag tagttggtgt agtcaaagct
     2461 ggtgctgccg ttgaagttgc ccccgttgcc ctgcaccatc ttggcgtggg ccagcttcgg
     2521 gaggctggcc gagccctcga acatcaggtg ctcgaagaga tgggcgaagc ccgtccggcc
     2581 ctcgggctcg gaacggaaac ccacgtcgta gtagacggcc accgtggccg acggcgacga
     2641 gcggtccggg acgaccacga cccgcaggcc gttggccagc tcgaagcgct cgacggtgaa
     2701 ctcgctgcgg gacgagggca ccgccggaca ctagcggccg gcggaaatgt tgagcgaact
     2761 ggtcgggttt actaacctga gccccaccac aacccaggag gtagtcatgg ccatccagct
     2821 cggagacacc gcccccgact tcaccgcaga caccaccgaa ggcacgctga ccttccacga
     2881 gtacctgggc gacggttggg gcatcctgtt ctcccacccc aaggacttc
//
LOCUS       DASWGG010000192         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_88974, whole genome shotgun sequence.
ACCESSION   DASWGG010000192 DASWGG010000000
VERSION     DASWGG010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88974"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..601
                     /locus_tag="VF954_03485"
     CDS             203..601
                     /locus_tag="VF954_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530175.1"
                     /translation="MAEQDEVRLALPATPEFLRLARVTATGLASRLGFTYDEVEDLRL
                     AIDELCFTLIGTRGRPGLLKLRYVLTGAALDVEGVGHFDDDHEGLEPKVSDLSRQILS
                     ALVEHHEVRRNTEGLLAFRLRKRHLDPVVR"
     gene            598..1350
                     /locus_tag="VF954_03490"
     CDS             598..1350
                     /locus_tag="VF954_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="HEX9530176.1"
                     /translation="MSLDPADRGELRSRFAEFAHSRDPALRDRLVEAHLGLAEYLARR
                     FSNRGEPLDDLIQVAAVGLLKAVDRFDPDRGVEFSTYATHTVVGELKRHFRDKGWAVR
                     APRRMQELYLRLGKVVANLSQELGRSPTIPELAAEAQVSAEEVLEAMEAGQAYRFASL
                     DAPSPGDDNGDSLGTHLGGDDPSMADAEQRATLSPLVSHLQPRERMIIHLRFFEGLTQ
                     SEIAARLGISQMHVSRLLARSLSQLRAAAEGD"
     gene            1380..>1851
                     /locus_tag="VF954_03495"
     CDS             1380..>1851
                     /locus_tag="VF954_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX9530177.1"
                     /translation="MRILLIVNTSASSVTARTRVVIGKALAADHRVTVAETSRRGHAT
                     RLAQSAAASGADVVVVLGGDGTLNEAANGLAGTPTALGALPGGSTNVFARTIGMTNDP
                     IEATGELLGALARGSVRPIGLGRVNGRYFLFHVGIGFDAAVVDQVERRSHLKRYA"
BASE COUNT          264 a          650 c          677 g          260 t
ORIGIN      
        1 catggccaca gattgttcac gacagacgcc cctctccacc aactgggggt ggcgggcggc
       61 gggccgcggg gtcgcggtgg gcccttcggt gggcttcggc gtcgcggggc ggttttggta
      121 gcgtcgtcag ccggagcggc agccctctgg ccaggcacgg tgagttcatg gccgtcgagg
      181 gctatcggca aggagagggc acgtggccga gcaggacgag gtgaggctgg ccctaccggc
      241 gacccccgag ttccttcggc tggcgcgggt cacggcgacg ggcctggcca gccggctggg
      301 gttcacctac gacgaggtcg aggatctgag gctcgccatc gacgagctgt gcttcaccct
      361 catcggcacc cgggggcggc ccgggctgct caagctgcgc tacgtgctga cgggtgccgc
      421 gctcgacgtc gaaggcgtag gccacttcga cgacgaccac gagggcctcg agccgaaggt
      481 ctcggacctg tcccggcaga tcctgagcgc gctggtcgag caccacgagg tccggcgcaa
      541 caccgagggg ctgctcgcct tcaggctgcg aaagcgccac ctcgacccag tggtgcggtg
      601 agcctcgatc cagccgaccg tggagagctg cggagccggt tcgccgagtt cgcccacagc
      661 cgcgacccgg cgctgcgaga ccggctggtc gaggcccatc tcggcctggc cgagtacctg
      721 gcgcgacgct tctccaaccg gggcgagccc ctcgacgacc tcatccaggt ggcggcggtc
      781 ggcctgctca aggcggtgga ccgcttcgat ccggaccgcg gggtggagtt ctcgacctat
      841 gcgacccaca ccgtcgtcgg cgagctgaag cgccacttcc gggacaaggg gtgggccgtg
      901 cgcgccccgc ggcgaatgca ggagctgtac ctgcggctcg gcaaagtcgt ggccaacctg
      961 tcccaggaac tggggcggtc gccgaccatc ccggagctgg cagccgaggc ccaggtgtcg
     1021 gcggaggagg tcctcgaggc catggaggcc gggcaggcct accggttcgc gtcgctcgac
     1081 gccccgagcc ccggcgacga caacggcgac agcctgggca cgcacctggg cggcgacgac
     1141 ccctcgatgg ccgacgccga gcagcgcgcc accctgtcgc cgctggtgtc gcacctgcag
     1201 ccacgcgaac ggatgatcat ccacctccgg ttcttcgagg ggctgacgca atccgagatc
     1261 gcggcgcgac tcgggatcag ccagatgcac gtctcccgcc tgctcgctcg aagcctctcg
     1321 cagctccggg ccgcggccga gggggactga acgggcggct gtggtcgaat ggctccctcg
     1381 tgcgcatcct gctcatcgtc aacacgtcgg cgtcgtcggt aaccgcccgg acccgcgtgg
     1441 tcatcggaaa ggccctggcc gccgaccacc gcgtcacggt ggccgagacg tcccggcggg
     1501 gccacgccac ccggctggcc cagagcgcgg ccgcgtcggg agccgacgtc gtggtggtcc
     1561 tcgggggcga cggcaccttg aacgaggccg ccaacggcct ggccggcacg cccaccgctc
     1621 tcggagcgct gcccggcggg tccacgaacg tcttcgctcg cacgatcggg atgaccaacg
     1681 acccgatcga ggccacgggc gagctgctcg gggcgttggc ccggggctcg gtccgtccga
     1741 tcgggctggg acgggtcaac gggcgctact tcctcttcca cgtgggcatc ggcttcgacg
     1801 ccgcggtggt ggaccaggtg gagcgccgct cgcacctgaa gcggtatgcc g
//
LOCUS       DASWGG010000193         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1805607, whole genome shotgun sequence.
ACCESSION   DASWGG010000193 DASWGG010000000
VERSION     DASWGG010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805607"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VF954_03500"
     CDS             <1..288
                     /locus_tag="VF954_03500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9530178.1"
                     /translation="PTVAAHWSRPLALLFVDGGHGPEPAWADYRGWAPHVAEGGLLAI
                     HDVFPDPAAGGRPPYELFCHAVASGDFEEQPALSYGSLRVLRRVGGAGPPR"
     gene            complement(380..1447)
                     /locus_tag="VF954_03505"
     CDS             complement(380..1447)
                     /locus_tag="VF954_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530179.1"
                     /translation="MLSASELRSTAASIGEAQLPGGMIPWFAGGHADVWNMVEAAMAL
                     ATAGFTYEAGRAYQWLADAQDAGGAWHHYYVAGGVEEARRDTNCCAYVATGVWHHYLA
                     TGDAGFLEAMWPVVEAALAFVLDHERPGGEILWCREADGSPGRFALLTGCSSIYFSLR
                     CAIAAAEHQGMERPDWELAAGRLAHAVAFRPDCFEPKDRWAMDWYYPVLAGVLTAPAG
                     RARLRERWSEFVLEGRGVRCVADRPWVTAAETAELVMALEAVGCTDEARSLFAWAQQL
                     RHHDGSYWTGCVYPEAKHFPGGERSSYTAAAMILAADVLAGPAGSPASGLFRGEGLPR
                     SASLDLDLDAVDAVAERSDRH"
     gene            complement(1441..>1636)
                     /locus_tag="VF954_03510"
     CDS             complement(1441..>1636)
                     /locus_tag="VF954_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9530180.1"
                     /translation="PYWWLRCAVGLERDDHPLVKAYHSVLVWDITSAPWPTRLAERLL
                     NPWLGKSLVVYASKPGGQPC"
BASE COUNT          220 a          642 c          575 g          199 t
ORIGIN      
        1 cccaccgtcg ccgcccactg gtcgcgccca ctggccctgc tcttcgtcga cggcgggcac
       61 ggcccggagc cggcgtgggc cgactaccgg ggctgggccc cgcacgtcgc cgagggcggg
      121 ctgctcgcca tccacgacgt gttccccgac ccggccgccg gtggccggcc gccgtacgag
      181 ctgttctgcc acgccgtcgc ctcgggcgac ttcgaggagc agcccgccct gtcgtacggc
      241 agcctgcgcg tcctccgccg cgtgggaggc gccgggccac ccaggtgagc gcgtggcggc
      301 attcttggca ccgccaggtg ccatttttca cgcttttcgg tgccaggaac ccgaccacgt
      361 gggggccggg ctcgagccgt caatgccggt cggaccgctc cgccaccgcg tccaccgcgt
      421 ccaggtcgag atcgaggctg gccgaccggg gcaggccctc gccccggaac agccccgacg
      481 ccgggctgcc cgccggcccc gccaggacgt cggcggcgag gatcatcgcc gcggcggtgt
      541 acgagctgcg ctcgccaccg gggaaatgct tggcctccgg gtacacgcag ccggtccagt
      601 aggagccgtc gtggtggcgc agctgctggg cccaggcgaa gagcgagcgc gcctcgtcgg
      661 tgcagcccac ggcctccagg gccatcacca gctcggccgt ctcggcggcc gtgacccacg
      721 gccggtcggc gacgcagcgc acgcccctgc cctcgagcac gaactccgac caccgctccc
      781 tcagccgggc gcgccccgcc ggggccgtga ggaccccggc caggacgggg tagtaccagt
      841 ccatggccca gcggtccttg ggctcgaagc agtcggggcg aaaggcgacc gcgtgcgcca
      901 accggcccgc ggccagctcc cagtcgggcc gctccatccc ctggtgctcg gcggccgcga
      961 tggcgcagcg caggctgaag tagatcgacg agcagcccgt caacagggcg aagcggccgg
     1021 gcgagccgtc cgcctcgcgg caccacagga tctccccgcc cgggcgctcg tggtcgagca
     1081 cgaacgccaa ggccgcctcc accaccggcc acatggcctc gaggaagccg gcgtcgcccg
     1141 tggcgaggta gtggtgccac acaccggtcg cgacgtaggc acagcagttg gtgtcccgcc
     1201 gggcctcctc gacgccgccg gccacgtagt agtggtgcca cgccccaccg gcgtcctggg
     1261 cgtccgcgag ccactggtag gcccgccccg cctcgtacgt gaagccagcg gtggccagcg
     1321 ccatggccgc ctccaccatg ttccacacgt cggcgtggcc gcccgcgaac cacgggatca
     1381 tgccgccggg cagctgcgcc tccccgatcg acgccgccgt ggaccgcagc tcgctggcgc
     1441 tcagcacggt tgaccccccg gcttcgaggc atagacgacc aggctcttgc ccagccacgg
     1501 gttgagcagc cgctcggcca ggcgggtggg ccacggcgcc gaggtgatgt cccacaccag
     1561 caccgagtgg taggccttga ccagggggtg gtcgtcacgc tccaggccga cggcgcagcg
     1621 cagccaccag tacggg
//
LOCUS       DASWGG010000194         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3090848, whole genome shotgun sequence.
ACCESSION   DASWGG010000194 DASWGG010000000
VERSION     DASWGG010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3090848"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VF954_03515"
     CDS             complement(<1..194)
                     /locus_tag="VF954_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530181.1"
                     /translation="MTLIILFVVAAAVLAAATVGAALLVGRRPTAAPAPPKAPPTAPP
                     KAPPTAPPKAPPTAPPKAPP"
     gene            complement(282..>2227)
                     /locus_tag="VF954_03520"
     CDS             complement(282..>2227)
                     /locus_tag="VF954_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9530182.1"
                     /translation="VVADARAAERALQAAGPGEPLGILVATGSDDPMLGGRPLTDLLH
                     PISGPAAAALAGVYLARGSGEAARLAGEHPEAVFVTLDGVMATGRLLVSTSAAAGARA
                     EEAETELAAARERLSATDAEIAAVRRSFEEAAAVLNRADAEIAAASDRAASAERELHA
                     LDREAGVVDEGDQRSAASASTLAERIAALQEGLADGQRRAEAAGRELEAYADEQVSAA
                     RVRGEAAGALDEARLAAARAAERHRLLVERCSELRATLDRATAQAAAIGGRRADLAAR
                     AGRAASVFRAAGILAERAGAWAVEADAAHLAWGREAAEADAGVTALQDRRRSLVAALE
                     ALREKARAEDLSRAELRIRSRIIEERLSAEGRDVEALVRRFGRRLEDEDPASLVDPWD
                     RAAVAEDEALQRRQARLERDLAAMGRVNPLAAAEFESLTEREQFLTAQIADVRTSRRD
                     LLKVVTEVDERIRELFLGAFADVSREYEHVFSTLFPGGVGRLRLTDPADVLETGIEVE
                     ARPGGKNLRRLSLLSGGERALAGLALAFAIFRARPSPFYVLDEVEAALDDVNLHRFLG
                     LLREFRSSSQLIVVTHQKRTMEMADVLYGVSVRSDGASRVISERLSSRSGSPPDPGGE
                     QRGELGRGPGGLEFTARPLPLEG"
BASE COUNT          284 a          859 c          811 g          273 t
ORIGIN      
        1 ctgggcgggg ctttcggagg cgccgtgggc ggggctttcg gaggcgccgt gggcggggct
       61 ttcggaggcg ccgtgggcgg ggctttcgga ggggcggggg ccgcggttgg gcgcctgccg
      121 acgagcagag cggcgccgac cgtcgccgcc gccaggacgg cggccgcgac gacgaacagg
      181 atgatgagcg tcacggcgcc tgcctcgtgt tcacaaaccc tctattctcg atgcgccccc
      241 gcgcacgcga cagcgacggc cgggcacaac aacgtcgtgg cttagccttc cagcgggagt
      301 gggcgggcgg tgaattcgag accgcccggc ccccggccca attccccacg ctgctcccca
      361 ccaggatccg ggggtgaccc cgagcgggaa ctcagccgct ctgagattac ccggctggcg
      421 ccatcggagc gaaccgaaac accgtagagg acatcggcca tctccatggt gcgcttctgg
      481 tgggtgacca cgatcagctg cgaactgctc cggaactccc ggagcaggcc gaggaaccgg
      541 tgcaggttca catcgtccag ggcggcctcc acctcgtcga gcacgtagaa cggtgacggg
      601 cgtgcccgga agatcgcgaa ggcgagcgca agccctgcga gcgcccgctc gccaccggac
      661 agcaacgaca ggcggcgcag gttcttgccg cccgggcgtg cctcgacctc gatgcccgtc
      721 tcgaggacgt cggcggggtc cgtcaggcgg aggcggccca cgccgccggg gaacagggtc
      781 gagaacacgt gctcgtactc gcgggacacg tcggcgaagg cgcccagaaa cagctcacgg
      841 atgcgttcgt cgacctcggt caccaccttc aagaggtccc ggcggctggt ccggacgtcg
      901 gcgatctggg cggtcaggaa ctgctcccgc tcggtgagcg actcgaactc ggcagcggcg
      961 agggggttca ccctccccat cgccgccagg tcccgctcca ggcgggcctg gcgccgctgc
     1021 agggcctcgt cctcggcaac cgcggcgcgg tcccagggat ccacgagcga cgctgggtcc
     1081 tcgtcctcca ggcgtcgccc gaagcgccgg acgagggcct cgacgtcccg gccctccgcc
     1141 gagaggcgct cctcgatgat gcgggaccgg atgcgaagct ccgcccgcga caggtcctcg
     1201 gcccgggcct tttcccggag ggcctccagc gccgccacga gcgagcggcg gcgatcctgc
     1261 aacgccgtga cgccggcgtc ggcttcggcc gcctcccggc cccaggccag gtgggcggcg
     1321 tccgcctcga ccgcccaggc ccccgcccgc tccgccagga tcccggcggc ccggaacacc
     1381 gaggccgccc ggccggctcg ggccgccagg tccgccctgc ggcccccgat ggcggcggcc
     1441 tgggcggtcg cccggtcgag ggtagcccgg agttccgagc accgctccac cagcagccgg
     1501 tggcgctccg cggcccgggc tgccgccagc cgtgcctcgt cgagggcacc ggcagcctcc
     1561 ccccggaccc gggctgccga cacctgctcg tctgcgtagg cctcgagctc cctcccggcg
     1621 gcctcggcac ggcgctggcc atcggcaagg ccctcctgca gggcggcgat ccgctctgcg
     1681 agcgtggatg ccgaggcggc gctgcgctgg tccccctcgt ccaccacccc ggcctcgcgg
     1741 tcgagggcat ggagctcccg ctccgccgat gccgcccggt ccgaagcggc ggcgatctcc
     1801 gcatccgccc ggttcagcac ggcagccgcc tcctcgaagc tccggcgcac cgcggcaatc
     1861 tcggcgtccg tggccgagag gcgctccctc gccgccgcca gttcggtctc cgcctcctcc
     1921 gcgcgggcgc cggccgccgc ggacgtgctg acgagcagcc gtccggtggc catcacgccg
     1981 tcgagggtga cgaacacggc ctcggggtgc tcccctgcca gccgggccgc ctccccggat
     2041 ccgcgggcga ggtagacgcc cgcgagggcg gcggccgccg ggccggagat ggggtggagc
     2101 aggtcggtca ggggcctccc gccgagcatc gggtcgtcgg acccggtggc gacgaggatc
     2161 cccagcggct cgccgggccc cgccgcctgc aacgcccgct cggcggctcg ggcgtcggcg
     2221 accacca
//
LOCUS       DASWGG010000195         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_834770, whole genome shotgun sequence.
ACCESSION   DASWGG010000195 DASWGG010000000
VERSION     DASWGG010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834770"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VF954_03525"
     CDS             <1..854
                     /locus_tag="VF954_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530183.1"
                     /translation="DHSVVEELVREGQISPEEAAVHPQRHVVTRALGIGPGVEPDVWQ
                     ILPYQGDRILLCSDGLPNEVPEDEIASTLRREPDPEVAAARLVALARQHGGADNITVV
                     VVDVVDDDDRARSASAAVGSGERADRRDGQDSGAEHALDARPHRKAAGVEDLSPPVSA
                     ATPLPVAKPRHRRLTFRVALFVVVLVAVLGAGAAAVWFYGRGGYYVGLDRHQVTIYKG
                     RPGGLLWIQPTVAEHTDLTDATVLPAEVAGLRGGHTEASLGAARHFVANLRSEAPAAA
                     PAAGSGP"
     gene            855..2300
                     /locus_tag="VF954_03530"
     CDS             855..2300
                     /locus_tag="VF954_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX9530184.1"
                     /translation="MDRQIRRLGIVLVVAFGVLFLQLNRLQIVDARRLANATGNTRNA
                     TRDFSRARGAIQAADGTVLAQSVPSHDAFSLQRQYPTGELFAGVTGYFSFTYGTDGVE
                     RTYNDDLSGKTLPVRKLSDLLTTRTRTATVTLTLDPRIQLAAKNALGIRRGAVVALDP
                     TNGSILALWSASSYDPNRLAAHDQNQVRQAWAGYQRQADKPMLPRAYRERYPPGSTMK
                     VMDTAAVLDQAPDLLSRTYPTLSQLPLPQSDKPLHNFGGETCGGPIPEMLVISCDTGF
                     AQVGLDLGPKKLTSEAHAFGLDQAPPLDLPAPAASAFPNAAAFTRNLPGLAYSAIGQQ
                     DVAATPLEMALVAAAIADNGVIQKPHVMAEIRDSEGNLVRQWSPGQWLQATSAQTAQA
                     VRDMMIQVVQRGTATNAQIPGVKVAAKTGTAQTGRNTTDAWLIAFAPADAPKVAIAVI
                     VEDQPPQGEVGTGGTVAAPIARAVMSAVLGK"
     gene            2321..>2991
                     /locus_tag="VF954_03535"
     CDS             2321..>2991
                     /locus_tag="VF954_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9530185.1"
                     /translation="MAEAAPRVFNDRYELVRHIARGGMAEVYLARDLRLDRYVALKIL
                     FPELSTDRAFVERFRREAQAAAGLSHPNVVSVYDWGEEDATYFIVMEYVDGQTLSQVI
                     RAEAPVDADWAAGVGADVAAALAFAHRNGVVHRDVKPGNVLITEDGHVKVADFGIARA
                     ASSQENLTQTGAVMGTATYFSPEQARGEAVDARSDVYSLGVVLYEMVTGRPPFSGDSP
                     VAIAY"
BASE COUNT          442 a         1159 c         1013 g          377 t
ORIGIN      
        1 aggaccacag cgtggtcgag gagctggtca gggagggcca gatatcgcca gaggaggcgg
       61 cggtccaccc gcagcgccac gtcgtgacgc gcgccctcgg catcggcccg ggggtcgagc
      121 ccgatgtgtg gcagatcctg ccctaccagg gcgaccgcat cctgctgtgc agcgacgggt
      181 tgcccaacga ggtgccggag gacgagatcg cctccaccct ccgccgcgag cccgaccccg
      241 aggtggccgc ggcccggctc gtcgccctcg cccgccagca cggcggggcg gacaacatca
      301 ccgtggtcgt cgtcgacgtc gtggatgacg acgaccgggc ccgatcggcc agcgcggcgg
      361 tggggtcggg agaaagggcc gaccgtcgcg acggacagga cagtggcgcc gagcacgccc
      421 tcgacgcccg gccgcaccga aaggcggccg gggtcgagga cctgtcgccg cccgtctcgg
      481 ccgccactcc cctaccggtc gccaaacccc gccaccgccg gctgaccttt cgggtcgcgc
      541 tcttcgttgt ggtgttggtg gctgtgctcg gcgcgggggc tgccgcggtt tggttctacg
      601 ggcggggcgg gtactacgtg ggcctcgacc gccatcaggt gaccatctac aagggccggc
      661 cgggcggcct gctgtggatc caaccgaccg tggccgagca caccgacctc accgacgcca
      721 ccgtcctgcc cgcggaggtg gcggggctgc gcggcgggca caccgaggcg tccctgggcg
      781 ccgcccgcca tttcgtcgcc aacctccgca gcgaggcgcc cgccgcggcg ccggcggccg
      841 gctccggccc ctagatggac cgccagatcc gccgcctggg gatcgtgctg gtggtggcct
      901 tcggcgtgct gttcctccag ctgaaccggc tccagatcgt cgacgccagg cggctggcca
      961 acgcgaccgg caacacccgc aacgccaccc gcgacttcag ccgggcccgg ggcgccatcc
     1021 aggccgcgga cggcacggtg ctggcccagt cggtgcccag ccacgacgcc ttcagcctcc
     1081 agcgccagta cccgaccggt gagctgtttg ccggggtgac cggctacttc tccttcacct
     1141 acggcaccga cggcgtggaa cgcacctaca acgacgacct cagcggcaag acgctgccgg
     1201 tccgcaagct gagcgacctg ctcaccaccc ggacccgcac cgccaccgtc accctcaccc
     1261 tcgatcccag gatccaactg gccgccaaga acgccctcgg catccgccgg ggcgcggtcg
     1321 tcgccctcga ccccaccaac ggctccatcc tcgccctgtg gagcgcatcc tcctacgacc
     1381 ccaaccggct ggcggcccac gaccagaacc aggtccgcca ggcctgggcc ggctaccaga
     1441 ggcaggccga caagccgatg ctcccccggg cctaccggga gcgctacccg ccgggctcga
     1501 ccatgaaggt gatggacacc gcggcggtac tcgaccaggc gccggacctg ctctcccgga
     1561 cgtacccgac cctcagccag ctgccgctgc cccagtcgga caagcccctg cacaacttcg
     1621 ggggcgagac ctgcggcggg ccgatcccgg agatgctcgt gatctcctgc gacaccggct
     1681 tcgcccaggt cggcctcgac ctcggcccca agaagctgac ctccgaggcc cacgccttcg
     1741 gcctggacca ggcgccgccc ctcgacctgc cggcgccggc ggcgtcggcg ttcccgaacg
     1801 cggcggcctt cacccgcaac ctgcccgggc tggcctactc ggccatcggc cagcaggacg
     1861 tggccgccac cccgctcgag atggcgttgg tggccgccgc catcgccgac aacggcgtca
     1921 tccagaagcc ccatgtcatg gccgagatcc gcgacagcga gggcaacctg gtccgccagt
     1981 ggagcccggg ccagtggctc caggcgacct ccgcgcagac ggcgcaggcg gtgcgggaca
     2041 tgatgatcca ggtcgtgcag cggggcacgg ccaccaacgc ccagatcccc ggcgtgaagg
     2101 tggcggccaa gacgggcacc gcgcagaccg gccgcaacac gaccgacgcc tggctcatcg
     2161 ccttcgcccc ggccgacgcc cccaaggtgg cgatcgccgt catcgtggag gaccagcccc
     2221 cgcaggggga ggtcggcacc ggcgggacgg tggcggcgcc catcgccagg gcggtcatgt
     2281 ccgcagtgct gggcaagtga agccttaggc tgggtgggcc atggcagaag ccgccccccg
     2341 ggtgttcaac gaccgctacg agctcgtccg ccacatcgcc cgcggcggga tggccgaggt
     2401 ctacctggcc cgcgacctgc gcctcgaccg gtacgtggcg ctcaagatcc tcttcccgga
     2461 gctgtccacc gaccgggcgt tcgtcgagcg cttccgccgg gaggcgcagg ccgccgccgg
     2521 cctgtcgcat cccaacgtgg tctccgtgta cgactggggc gaggaggacg ccacctactt
     2581 catcgtgatg gagtacgtgg acggccagac cctctcccag gtgatcaggg cggaggcgcc
     2641 ggtcgacgcc gattgggccg ccggcgtcgg cgccgacgtg gccgcggccc tggccttcgc
     2701 ccaccgcaac ggcgtcgtgc accgcgacgt caagcccggc aacgtcctga tcaccgagga
     2761 cggccacgtg aaggtggccg acttcggcat cgcccgggcg gccagctcgc aggagaacct
     2821 gacccagacc ggcgccgtga tgggcaccgc cacctacttc tcccccgagc aggctcgcgg
     2881 cgaggccgtc gacgcccgca gcgacgtgta ctccctcggc gtggtcctct acgagatggt
     2941 gaccggccgg ccgcccttct ccggggacag ccccgtggcc atcgcctaca a
//
LOCUS       DASWGG010000196         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1476953, whole genome shotgun sequence.
ACCESSION   DASWGG010000196 DASWGG010000000
VERSION     DASWGG010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476953"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VF954_03540"
     CDS             <1..990
                     /locus_tag="VF954_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9530186.1"
                     /translation="RLPRPRGRRVSTRGPGTRPPAPAPSHVASKAAVLRRLELDVTRR
                     LDGLLSGDHLAASSGPGTEPAGAREYGPGDDARRIDWSLTARSLGPHVRTTEADRELE
                     TWLVIDRSPSLDFGTARWEKREVALAAMAAFGFLALRSGNRLGVVVAGIDQLLVVPAR
                     AGRPHLMAALSTVHDTRRREDRPAPRADLTGGLERVRHLKSRRGQVVVISDFLDPPGW
                     SLPLRGLVLRHQVVAVHVTDPRELTLPAVGLLGVVDPETGRRLHVQSDSERLRERFSV
                     AAASWRAEIRRTILHSGAEYLDLGTERDWLVDVARFIRSRRAARRHVKIGGVR"
     gene            987..1955
                     /locus_tag="VF954_03545"
     CDS             987..1955
                     /locus_tag="VF954_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9530187.1"
                     /translation="MTFLDLSRLALLLVPAGLVVAYLLVQRVRARTLVRFTNVDLLAS
                     VAPRRPGWQRHIPAAALVAALALMVVGLAHPARVVRTPRQKATVVLTLDTSGSMIADD
                     VSPTRLGAAQQAARTFVQALPPGVQLGLVKFESTAEVLVSPTTDRATVLAAINQLTAS
                     GGTATGDGLDLALQAINTVPRAANGKPVPAAIVLMSDGVPMIGHGGLSPSEAVAAAAA
                     SARQQGVKVHTIAYGTQEGAIRFGDRVVAVPSDPAAMAAIASATGGHTFSARSSGQLK
                     SVYRAIGRAVGYDVHRKDVAAWFTVLAFVVAALAAAAALYWTQRIA"
BASE COUNT          252 a          825 c          746 g          281 t
ORIGIN      
        1 cgacttcccc gcccgcgagg ccgccgggtg agcacccggg gaccgggcac ccggccgcct
       61 gcacctgcgc cctcgcacgt ggccagcaag gccgccgtgc tgcgccggct cgagctggat
      121 gtcacccgcc ggctcgacgg cctgctcagc ggcgaccatc tcgcggccag ctccgggccc
      181 gggaccgagc cggccggcgc ccgggagtac ggaccgggag acgacgcccg ccgcatcgac
      241 tggagcttga cggcccggtc gctcgggccg cacgtgcgca ccaccgaggc cgaccgggag
      301 ctggagacct ggctggtgat cgaccggtca ccgagcctcg acttcggaac ggcccggtgg
      361 gagaagcgag aggtcgcgct ggccgccatg gccgcgttcg gcttcctcgc cctgcgctcg
      421 ggcaaccgtc tgggcgtcgt ggtggcgggc atcgaccagc ttctcgtggt cccggcccgc
      481 gccgggcggc cccacctgat ggccgccctg tcgacggtgc acgacacccg ccggcgggag
      541 gaccggcccg cgccgcgcgc cgatctgacc ggcgggctgg agcgggtgcg gcacctcaaa
      601 tcccggcgag gccaggtggt ggtcatctcg gacttcctcg acccgcccgg ctggtcactc
      661 cccctgcggg gcctggttct gcgccaccag gtcgtcgccg tccacgtgac cgacccgcga
      721 gagctcaccc tgccggcggt cggcctgctg ggcgtggtgg accccgagac cgggcgacgg
      781 ctccacgtcc agagcgactc cgagcgactg cgagagcggt tctccgtcgc agccgcctcc
      841 tggcgcgccg agatccgccg caccatcctc cactccgggg ccgagtacct ggatctcggc
      901 accgagcgcg actggctggt ggacgtggcc cgcttcatcc ggtcgcgtcg ggccgcccgt
      961 cgccacgtca agatcggcgg cgtgcgatga ccttcctgga cctctcccgg ctggccctgc
     1021 tgctcgttcc cgccgggctg gtggtcgcct acctgctcgt gcagcgggtg agggcgcgca
     1081 cgctggtccg cttcaccaac gtcgacctgc tggcctccgt ggcgccccgc cggcccgggt
     1141 ggcagcgcca catcccggcg gccgccctcg tcgccgccct ggccctgatg gtcgtcggtc
     1201 tcgcccaccc ggcccgggtg gtgcgcactc cgaggcagaa ggccaccgtg gtcctcacgc
     1261 tagacacttc cgggtccatg atcgccgacg acgtgagccc gacccggctc ggtgcggccc
     1321 agcaggcggc ccgcacgttc gtgcaggccc tgccaccggg cgtgcagctg gggctggtga
     1381 agttcgagtc cactgccgag gtgctggtct cgcccaccac ggaccgggcg acggtgctgg
     1441 cggccatcaa ccagctcacc gccagcgggg gcacggccac cggcgatggc ctggacctgg
     1501 ccctgcaggc catcaacacc gtgccccggg ccgccaacgg caagcccgtg ccggccgcca
     1561 tcgtgttgat gagcgacggc gtcccgatga tcggccacgg cggactttcg cccagcgagg
     1621 cggtggcggc cgcggccgcg tcggccaggc agcagggcgt caaggtccac accatcgcct
     1681 acgggaccca ggagggcgcc atcagatttg gcgaccgcgt cgtggccgtc ccgtccgacc
     1741 cggccgccat ggccgccatc gcctcggcga cgggcggcca cacgttctcg gccaggtcgt
     1801 cggggcagct caagtcggtg taccgggcca tcggccgcgc cgtcggctac gacgtgcacc
     1861 gcaaggacgt ggccgcgtgg ttcacggtcc tggccttcgt cgtcgccgcc ctggccgcgg
     1921 cggccgccct ctactggacc cagcgaatcg cgtgagtgat gtgagcgaga tgacaaccac
     1981 gtggagcgaa cctctcgact ggctcccgcc ggagcccccg cccccgggcg ggccgggccg
     2041 gccaccccct ggggggccac cacctggcgg gccgccacct catggccggc cctccggact
     2101 gcgc
//
LOCUS       DASWGG010000197         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_834899, whole genome shotgun sequence.
ACCESSION   DASWGG010000197 DASWGG010000000
VERSION     DASWGG010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834899"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VF954_03550"
     CDS             complement(<1..366)
                     /locus_tag="VF954_03550"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9530188.1"
                     /translation="MKLLPRRSAGRTTVRRLYWWREALTVLVLYEVYSAIRNLSEGST
                     RQAFRHARQLMHWQHMIGLNHEATLQSWALHSKPLVIVLNYVYGSLHFIITTGAVVWL
                     YRRHPGDYPLWRNTLALTTV"
     gene            complement(368..1288)
                     /locus_tag="VF954_03555"
     CDS             complement(368..1288)
                     /locus_tag="VF954_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX9530189.1"
                     /translation="MASAVMLALLLPRMHLPSLLPPGHHGDTIVYLLLGLLTTLGGIV
                     LSAWRWQRVLAVFDADTRLPSLVSHYLAGQFVGNFLPSTIGGDVLRVSRLTNSSDVPG
                     TSVFASVVIERLTGWIVLPLLTLTGLLLRPSLLRLGVASRLALALSIGTLLLLAGIMA
                     AAGSRRVAGRFAANEGWTRFIGAVHLGVDSLRRRPQAAAGILGVATVYQLSPVLAAWF
                     ATRALGLHLPMMALLAFTPVVAIAQVLPLSLNGLGIREGAFVLFLGPLGVSAGQAVAV
                     GLIVYALTLAVSLLGAPAFAVGGRSRAVSI"
     gene            1431..>1691
                     /locus_tag="VF954_03560"
     CDS             1431..>1691
                     /locus_tag="VF954_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9530190.1"
                     /translation="MDSFGGHPQTPGLGGHSAAPEFVHGGTLAAGALTAAGVDVVFTL
                     SGGHIFPLYDGCVAAGIRLVDTRHEQTAGFAAEGWAKVTRRPG"
BASE COUNT          279 a          559 c          661 g          192 t
ORIGIN      
        1 cacggtggtg agggccagcg tgttgcgcca cagggggtag tcgccggggt ggcggcggta
       61 gagccacacc accgcgccgg tcgtgatgat gaagtggagc gacccgtaca cgtagttgag
      121 gacgatgacg agcggcttgg agtgcagcgc ccaggactgc agcgtcgcct cgtggttgag
      181 gccgatcatg tgctgccagt gcatgagctg ccgggcgtgg cggaaggcct ggcgggtgct
      241 gccctcgctg aggttccgga tcgcggagta gacctcgtag agaacgagaa cggtcagcgc
      301 ctcgcgccac cagtacaggc gccggacggt ggttcggccg gccgatctcc gaggcagcag
      361 cttcacttca gatggagacc gcccggctgc ggcccccgac ggcgaaggcg ggggcgccga
      421 gcaggctcac cgccagcgtc agcgcgtaga cgatcaggcc cacggccacc gcctggccgg
      481 ccgagacgcc gagcgggccg aggaagagca cgaaggcccc ctcccggatg ccgaggccgt
      541 tgagcgacag ggggagcacc tgggcgatgg ccaccaccgg ggtgaaggcc agcagggcca
      601 tcatcggcag gtggaggccg agcgcccggg tggcgaacca ggcggccagc accggggaga
      661 gttggtagac ggtggccacg ccgaggattc cggcggcggc ctggggccgg cggcggaggg
      721 agtcgacccc gaggtggacg gcaccgatga agcgggtcca cccctcgttg gcggcgaagc
      781 gaccggccac gcggcggctg ccggcggcgg ccatgatccc ggccagcagg aggagcgtgc
      841 cgatcgacag ggccagggcc agccggctgg ccacccccag ccgcaggagc gacggccgga
      901 gcaggagccc ggtcagcgtc aggagtggaa ggacgatcca gcccgtcagc cgctcgatca
      961 ccaccgaggc gaagaccgac gtgcccggca cgtcggacga gttggtgagg cggctgaccc
     1021 gcaggacgtc accgccgatg gtcgacggga ggaagttccc cacgaactgg ccggccaggt
     1081 aatgggagac gaggctcggg aggcgggtgt cggcgtcgaa gacggccagg acccgctgcc
     1141 agcgccacgc cgacagcacg atgccgccga gggtggtgag gaggccgagg agcagataga
     1201 cgatcgtgtc gccgtggtgg ccggggggta acagtgacgg caggtgcatc cggggcagga
     1261 ggagcgccag catcacggcg ctggccacga tccggacggc cggccacacc gcgccggccg
     1321 ccgatggctc gcgccccggc ggagggtcag atgcggctcg gccctgcatc cgctttagcc
     1381 taatcgccgc agcccgggcg acatccggat ctgcgacagg aaggtcggga atggactctt
     1441 tcgggggaca cccccaaacc cccgggcttg gtggacactc cgcggccccc gagttcgtcc
     1501 acggcggcac gctggcggcg ggagctctga cggcggccgg cgtcgacgtc gtcttcaccc
     1561 tgtccggcgg gcacatcttt cccctctacg acggctgcgt ggcggccggc atccgcctcg
     1621 tcgacacccg ccacgagcag acggccgggt tcgccgccga gggctgggcc aaggtcaccc
     1681 gccggcccgg g
//
LOCUS       DASWGG010000198         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1806835, whole genome shotgun sequence.
ACCESSION   DASWGG010000198 DASWGG010000000
VERSION     DASWGG010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806835"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VF954_03565"
     CDS             complement(<1..969)
                     /locus_tag="VF954_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9530191.1"
                     /translation="MPRNPSERETAPDTSALMEDALFQIKRVIVGQDRMVERALVCLL
                     ARGHCLIEGVPGVAKTLTVSTMAHVVGGTFHRIQFTPDLVPADIVGTRIWRPSREEFD
                     IEWGPVFANIVLADEINRAPAKVQAALLEIMAERHVSIAGSTRTVPEPFLVLATQNPI
                     ESEGVYALPEAQRDRFLMQLEVGHPSYEEETEIARRMGVHPPSAETVLTAGQLIAIQS
                     EVDEVFVHHAVADYAVRVVMASRDPARWGLPDLAQHVALGASPRATLGLLSASRALAL
                     LRGRRFAVPQDVFDVAPDVLRHRLLLTYDGLAAGVQVNDVVARLLGT"
     gene            1536..>1825
                     /locus_tag="VF954_03570"
     CDS             1536..>1825
                     /locus_tag="VF954_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530192.1"
                     /translation="MALLVAGALVAGFLGEIEVRRHLHQRAATTSPTITIPATTVPST
                     PPDPAHSLLDSMVLKQADVAPDAVVGPVRDGIGLSAPTLDLCQASFPSEALRS"
BASE COUNT          260 a          657 c          660 g          248 t
ORIGIN      
        1 ggtcccgagg agccgagcca ccacgtcgtt gacctggacg cccgcggcca agccgtcgta
       61 ggtgagcagg aggcggtggc ggagcacatc gggcgccacg tcgaacacgt cctggggcac
      121 cgcgaaccgg cgcccgcgca gcagggcgag ggcccgtgac gccgagagca gcccgagggt
      181 ggcccggggg ctggccccga gggccacgtg ctgggccagg tccggcagcc cccagcgggc
      241 cgggtcgcgg ctggccatga ccacacgcac tgcgtagtcg gcgaccgcgt ggtggacgaa
      301 gacctcgtcc acctcggact ggatggcgat gagctgcccg gcggtcagca ccgtctcggc
      361 gctgggtgga tgcacgccca tgcgcctggc gatctcggtc tcctcctcgt aggaggggtg
      421 cccgacctcg agctgcatga ggaaccggtc ccgctgcgcc tcgggcaggg cgtagacgcc
      481 ctcggactcg atggggttct gggtcgcgag cacgaggaag ggttcgggca cggtgcgggt
      541 cgaacccgcg atggagacgt gccgctcggc catgatctcc aggagggcgg cctgcacctt
      601 ggccggcgcc cggttgatct cgtcggcgag cacgatgttg gcgaacaccg ggccccactc
      661 gatgtcgaac tcctcgcgcg acggccgcca gatgcgtgtc cccacgatgt cggccggcac
      721 cagatcgggc gtgaactgga tccggtgaaa ggtgcctccc accacgtggg ccatggtgga
      781 cacggtgagc gtcttggcca ccccgggcac gccctcgatc aggcaatggc cgcgcgccag
      841 gaggcacacc aacgctcgct cgaccatgcg gtcctggccg acgatgaccc gcttgatctg
      901 gaacagggcg tcttccatga gggcgctggt gtccggcgcg gtctcccgtt cgctcgggtt
      961 cctgggcatc agaaggctcc ttggatctct cggaggcacg ggggcacgcc gccacgctgg
     1021 caggcgaagc tgagaggccg gtccatggct gctgtgagtc agatgagaac ccgctcccag
     1081 gtcggtccca ggtgcgacat ccatcatggc agggaaggga aggaccccga acgggccccg
     1141 gtagcctgcg ggagaggagg ttttgcgtcg tggaccgagc gccggcaccg ggctggtacc
     1201 cagatccctg gcagcctgga catcaccgat actgggacgg ttcgggatgg accaccgacc
     1261 agttccccga ggtgccctcg acggtgccgc cgggccgctg ggccatcccg gccgccggga
     1321 ccggcgagcc gggcgcggcg ggcgggagtg gtgcgtggtc ggcgacggcc caagccgtga
     1381 cgacggccac gcctcctggc gtgcaccctc cgaccgcccc gcccccaccg cccggatgga
     1441 tgccgcccag gtgggcggcc ccacccgagc cgcccgcccc cgtggtcccg gaagccgcac
     1501 cggtcgcagg cccgtcccgt tggccacagg ccttggtggc cctcctcgtc gccggggccc
     1561 tggtggcggg cttcctcggt gagatagagg tccggcgcca tctccaccag cgggccgcca
     1621 ccacctcgcc gaccatcacc atccccgcca cgaccgtccc gtcgactccg cccgatcccg
     1681 cccactcgct gctcgacagc atggtgttga agcaggccga cgtggccccc gacgccgtgg
     1741 tcgggccggt acgcgacggt atcgggctca gcgcgcccac gctcgacctg tgccaggcga
     1801 gcttcccgag cgaggcgctg cggtc
//
LOCUS       DASWGG010000199         7703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3096999, whole genome shotgun sequence.
ACCESSION   DASWGG010000199 DASWGG010000000
VERSION     DASWGG010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7703
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3096999"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VF954_03575"
     CDS             complement(<1..355)
                     /locus_tag="VF954_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530193.1"
                     /translation="MIETTTDRTRRGLSILALGFALLLTAAGAGTGLAVASRVAHRAV
                     RLRAADRQQTAVAAAGQEDSVVADLRARLGAAAGTVGTLADPSRLRAAALHVSPQATR
                     AAVLDAGGQALSSWQA"
     gene            498..1514
                     /locus_tag="VF954_03580"
     CDS             498..1514
                     /locus_tag="VF954_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9530194.1"
                     /translation="MRIALLAPPWVPVPPPLYGGTELVIDLLARGYAAAGHDVRLFAT
                     GDSECPVPQAFVYERAQGTSIGMTESELRQVTAAYSALEGYDVIHDHTMLGPFLAPAG
                     RAQVVHTIHLPLEGDRLAIYERVPGHVRLVAVSKSQHRHVPQLNVATVIHHGIDAARF
                     PFQGQAGDYGLFLGRMAQEKGVDIAIRAAQKAGVPLVMAGKMRSAEEILYFEQRIKPL
                     MSDGVTYLGEVSDGEKIDLLRGALCLLFPTQWSEPFGMVMLEAMACGTPVLATIFGAV
                     PEVVTDGETGFLCRDVGEMAEAIGRCATFDRAACRRSVEVYFSAERMTSDYLNLFRLL
                     TGGA"
     gene            complement(1534..2994)
                     /locus_tag="VF954_03585"
     CDS             complement(1534..2994)
                     /locus_tag="VF954_03585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX9530195.1"
                     /translation="MAPRGTSTSTFGVGRRESHDSSEFYARFQAPEITTADAVAGPVR
                     LRQRCILGDARHMDRLEDNSVALVVTSPPYFAGKEYEEALGHGHIPGSYKEYLMLLRD
                     VFRECARALEPGGRIAVNVANLGRKPYRSLASDVIRILQDDLRLLLRGEVIWRNGEGS
                     SGSCAWGSYRSAANPVLRDTTERIVVASKGRFDRAQAVATRQELALPHESTITSDEFM
                     EATLDVWNIAPESARRVNHPAPFPVELPQRLIELYTYRGDLVLDPFMGSGTTLVAAVR
                     AGREHVGYDTDPAYVETASKRVGAELRARAGNLRPVGPDGDVAGPSQEGPDDVQTRAR
                     REGKAAKEIAERVLTRAGFRIVATASRVRGLGLTVDVTALDGHDDPWYFDVTGAFTTT
                     RAGLVRTDTLWRSLGRAHVLAGRGYRPLVLLTSQLPKSRSQGDRVLRAAGPAPFFDAM
                     EMLSASDQDRLAAYAGGGRSLRPLEGFWTGKELAGI"
     gene            complement(3086..3784)
                     /locus_tag="VF954_03590"
     CDS             complement(3086..3784)
                     /locus_tag="VF954_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9530196.1"
                     /translation="MSWTRLRQVALVARDLEPVVADLSDAFGLEVAFRDPGVASFGLH
                     NAVLPLGNQFVEVVSPVRPGTAAGRQLDRLGGDGGYMVICHTDDHARRRDRVAQLGVR
                     VAWQGEDRGYSIMQLHPADTGGSFLEIDFQPGGDDPHGPWMPAGPDWQRAVRAEVVDR
                     IAGVTVQSADAGKAAARWAEILETVVVDGIIALDNATITFVDGTVDSLVGVTVAGPKV
                     TGDRVIAGVRFAAA"
     gene            complement(3781..4551)
                     /locus_tag="VF954_03595"
     CDS             complement(3781..4551)
                     /locus_tag="VF954_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9530197.1"
                     /translation="MPEPETVHYEAAAGLARLTLNRPDAMNAMTNRMVREAHDALVAA
                     GADASVRVLVLTGAGSSFCPGADLKHFTSGGEDERLSARVFKVTTLLHEIPAVTMAAI
                     NGACAGAGLGWALACDLRVMAAGARLNTAFLDVAVAGDMAVPWSLPRLVGAGRARDLS
                     FAPRKVGADEALGIGLVGRVFPDESFRDETEAVLSALLRKSPTALLGLKQNYVAAERM
                     TFADFVDYEAERHLRLAGGPDTREAFRAFVEKREPRFT"
     gene            complement(4544..5359)
                     /locus_tag="VF954_03600"
     CDS             complement(4544..5359)
                     /locus_tag="VF954_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9530198.1"
                     /translation="MLGGSPMSVPEILVEVADPVALITLNRPETLNALTFPMLGAFRE
                     AVDAAATDPRVVGIVVTGTGRGFCSGLDSSALQQTTTAGSAGRMRTDAVPGIFTWLLE
                     VPKPIVSAVNGVAAGGGFVLAVMSDLRIASSRGSFVSVFSRRGLIAEHGTTWMVPRLV
                     GAGAALDLLWSSRKLDAAEAHRIGLVQQVVEPDDLIGAARRYIVELADNVSPASLADT
                     KRLVYAHLGVGYPSALREAEEVQYAALDRVDAKEGVAALIERRPPVFPRLGDA"
     gene            complement(5404..6195)
                     /locus_tag="VF954_03605"
     CDS             complement(5404..6195)
                     /locus_tag="VF954_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9530199.1"
                     /translation="MTYEQILTEQRGEIVLLTLNRPDRLNAWTPRMSAELGDAIEAAD
                     SDPGVGAVVVTGAGRGFCAGADVSEVFETQLLSGAAPAAAPAAPPRRRDWVELVRSTK
                     PIVAAVNGPAIGVGLTMILPFDRIVAAESAKLSLRFVKMGLVPELASSSLLPQRCGWG
                     AASDLMLSGRTVLGTEAAAIGLADEVAPDDAVLDVAMERARTYAENPTPQLRWIKQLL
                     TQNAGETDHKLVQSREVALLRQAYATPEHKEAVAAFLGKRKAIFR"
     gene            complement(6259..6564)
                     /locus_tag="VF954_03610"
     CDS             complement(6259..6564)
                     /locus_tag="VF954_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530200.1"
                     /translation="MSDTLEGLQSVDQSLQYVIQLLNQAVQCLDQLLEWNNQNQPDHQ
                     PDKLQADLADLQRDLHAATDSHNALAAKIGYMQQQLPATQAGSPEGAESESEDSGNN"
     gene            6706..>7703
                     /locus_tag="VF954_03615"
     CDS             6706..>7703
                     /locus_tag="VF954_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9530201.1"
                     /translation="MDDGVLEIREEPVPDPGAEEALVRISTAGVCHSDLHLARGDWAG
                     VRTPRLGHEAIGVVEALGPGGERYAQVGDRVILGLGGSGGGYWCGACEYCLRGQPRHC
                     AQTKGILGTFAEYFTVWSKSLVKLPDSVGDQEAPLACGGLTAYGAVKKLLKHQILPGR
                     PVAVIGAAGGLGHYAVQIASAFGYQVVGIDIGEDRLQFVRSLGAHLAVDAAEAAEVVQ
                     QQLGGVDAALVFSSKLAGFDLGLKLLRRGGLFVAVGIPASSEGNLQLNPFELFLREPT
                     LIYSAVGTVQDMRELVDLAAAGRVRSHVSRVGALSELPAIFDELQAGKYLGRAVIN"
BASE COUNT         1063 a         2771 c         2780 g         1089 t
ORIGIN      
        1 cggcctgcca gctcgatagg gcctggccgc ccgcgtcgag cacggccgcc cgggtggcct
       61 gcggggagac gtggagggcg gcggcgcgga gccggctggg atcggcgagg gtcccgaccg
      121 tccccgcggc ggccccgagc cgggcgcgca ggtcggcgac cacgctgtcc tcctggccgg
      181 ccgcggcgac ggcagtctgc tgccggtcgg ccgcgcgcag ccgcacggcg cggtgggcga
      241 cccgcgaggc cacggccagg ccggtgccag cgccggcggc ggtcaggagc agggcgaagc
      301 cgagggccag gatggacagc ccgcgacgcg tgcggtcggt ggtcgtttcg atcactgtct
      361 gcggttgtcg gcgaaaagga gcgccgggtt gaggacatcg ttctgtgttc ttccagcatt
      421 ttgtgagtcc cggcacgagg cgggcgggcc acgcggccga actttccccc ctccgaggcc
      481 gtggttcaat ggaacacgtg cgcatagcgc tgctcgcccc gccctgggtc ccggtcccgc
      541 caccgctcta cggtggcacc gagctcgtca tcgacctgct ggcccggggc tatgcggccg
      601 cgggccacga cgtgcggctc ttcgcgacgg gtgattcgga atgtcccgtg ccccaggcgt
      661 tcgtctacga gcgggcccag ggcacgagca tcggcatgac cgagtccgag ctgcggcagg
      721 tcacggcggc ctactccgcc ctcgagggct acgacgtcat ccacgaccac accatgctcg
      781 gacccttcct cgccccggcg ggccgcgccc aggtggtcca caccatccac ctgcccctcg
      841 agggcgaccg cttggccatc tacgagcggg tgcccggcca cgtccggctg gtggccgtct
      901 ccaagtccca gcaccggcac gtgccgcagc tcaacgtggc gactgtgatc caccacggca
      961 tcgacgcggc gcgcttcccg ttccagggcc aggccggcga ctacggcctg ttcctcggcc
     1021 gcatggcgca ggagaagggg gtggacatcg ccatccgggc ggcccagaag gcgggcgtgc
     1081 cgctggtgat ggcggggaag atgcgttcgg ccgaggagat cctgtacttc gagcagcgca
     1141 tcaagcccct catgagcgac ggggtgacct acctcggcga ggtcagcgac ggcgagaaga
     1201 tcgacctgct gcggggcgcg ctctgcctgc tgttcccgac gcagtggagc gagcccttcg
     1261 ggatggtgat gctcgaggcc atggcgtgcg gcacgcccgt gctggccacc atcttcggcg
     1321 ccgtccccga ggtggtgacc gacggcgaga ccggcttcct gtgccgggac gtgggcgaga
     1381 tggccgaggc catcggccgc tgcgccacct tcgaccgggc ggcctgccgg cgctccgtcg
     1441 aggtgtactt ctcggccgag cgcatgacgt cggactacct aaacctgttc cggctgctga
     1501 ccggcggggc ctgaaggtca cggtccggcc ccgtcagatg ccggccagct ccttgccggt
     1561 ccagaagccc tccaggggtc gaagcgaacg gccaccgccg gcgtaggccg ccagcctgtc
     1621 ctggtcgctc gccgagagca tctccatggc atcgaagaac ggcgccgggc cggcggcgcg
     1681 cagcacccgg tcgccctgac tccgggactt gggcagctgc gatgtcagca ggaccaacgg
     1741 tcggtagccc cggccggcga gcacgtgggc ccggcccagg gacctccaca gcgtgtcggt
     1801 ccggaccagt ccagcccggg tcgtcgtgaa cgccccggtc acgtcgaagt accacggatc
     1861 gtcgtgccca tcgagggcgg tgacgtcgac ggtcaggccc aggccccgca cccgggaggc
     1921 ggtggcgacg atgcggaacc ccgcacgggt gaggacgcgc tcggcgatct ccttggcggc
     1981 cttgccctcc cgccgggcgc gcgtctgaac gtcgtcgggc ccttcctgcg acggcccggc
     2041 cacatcgcca tcggggccaa ccggtcgcag gttcccggcc cgggcccgca gctcggcgcc
     2101 cacccgcttg gatgccgtct cgacgtaggc gggatcggtg tcgtagccca cgtgctcgcg
     2161 gccggcgcgg acggccgcca cgagcgtcgt gccggacccc atgaacgggt cgaggaccag
     2221 gtcgccccgg taggtgtaga gctctatgag gcgctggggc agctcgacgg ggaagggagc
     2281 cgggtggttg acccggcgtg cgctctccgg ggcgatgttc cagacgtcga gcgtcgcctc
     2341 catgaactcg tcggacgtga tggtgctctc gtgcggaagg gcgagctcct ggcgcgtggc
     2401 gaccgcctgg gcccggtcga agcggccctt gctggccacg acgatgcgct cggtcgtgtc
     2461 cctgagcacc gggttggcgg cgctgcggta cgagccccat gcgcacgacc cggacgaccc
     2521 ctcgccgttc ctccagatca cctcgccccg gagcagcagc cggaggtcgt cctgaaggat
     2581 ccggatcacg tccgaggcca ggctgcggta gggcttgcgg cccaggttgg cgacgttgac
     2641 ggcgatccgc ccgccgggct ccaacgcccg cgcgcactcg cggaagacgt cccgaagcag
     2701 catcaggtac tccttgtagc tgcccgggat gtggccgtgg cctagggcct cctcgtactc
     2761 cttgcctgcg aagtacggcg gggaggtgac caccagggcg accgagttgt cctccagccg
     2821 gtccatgtgc cgggcgtcgc cgaggatgca gcgctggcgg agccggaccg gcccggccac
     2881 ggcgtcggcg gtggtgatct cgggcgcctg gaagcgggcg tagaactcgg acgagtcgtg
     2941 gctctcccgc ctgccgacgc cgaatgtaga ggtggacgtc ccccgcggcg ccacggcgcg
     3001 accctcaagc aatggtcctc ctcggctcgg cctgcgctcg cagggtacgc gagcgcaggg
     3061 gccagctggc ggatcaggcg gcgggtcagg cggcggcgaa cctcacgccg gcgatcaccc
     3121 ggtcgccagt gaccttcggg ccggcaaccg tgacgccgac cagcgagtcg accgtgccgt
     3181 cgacgaacgt gatcgtggcg ttgtccagcg cgatgatgcc gtcgacgacc acggtctcga
     3241 ggatctcggc ccagcgggcc gcggccttgc cggcatcggc cgactgcact gtcacgcccg
     3301 cgatcctgtc caccacctcg gcgcgcacgg cgcgctgcca gtccgggccg gccggcatcc
     3361 acgggccgtg agggtcgtcg ccgccgggct ggaagtcgat ctccaggaac gaccctccgg
     3421 tgtcggccgg gtggagctgc atgatcgagt acccgcggtc ctccccctgc cacgccacgc
     3481 ggacgccgag ctgggcgaca cggtcccgcc ggcgggcatg gtcgtcggtg tggcagatca
     3541 ccatgtaccc gccgtcgcct cccagacgat cgagctggcg cccggccgcc gtgccggggc
     3601 ggaccggcga gaccacctct acgaactggt tcccgagggg gagcacggcg ttgtgcaagc
     3661 cgaagctggc gacgccggga tcgcggaagg ccacctccag cccgaaggcg tcgctgagat
     3721 cggccaccac cggctcgagg tcgcgggcga cgagggccac ctgccgcagc cgggtccagc
     3781 tcacgtgaac ctcggctccc gcttctccac gaaggcgcgg aacgcctccc tggtgtccgg
     3841 gccccccgcc agccgcaggt gccgctccgc ctcgtagtcg acgaagtcgg cgaaggtcat
     3901 gcgctcggcg gccacgtagt tctgcttcaa tccgagcagc gccgtcgggg acttcctgag
     3961 cagcgccgac agcacggcct cggtctcgtc gcggaacgac tcgtcgggaa acacccgacc
     4021 gacgagccct atgccgaggg cctcgtccgc ccccaccttg cggggggcga aggacaggtc
     4081 acgggcccgg ccggcgccga cgagccgtgg gagcgaccag gggacggcca tgtcgcccgc
     4141 caccgccacg tcgaggaacg ccgtgttgag gcgcgccccc gccgccatga cgcgcaggtc
     4201 gcacgccagg gcccagccga gcccggcgcc cgcacacgcg ccgttgatgg cggccatggt
     4261 gacggccggg atctcgtgga gcagggtggt caccttgaac acgcgggcgc tgaggcgctc
     4321 gtcctctccc ccactcgtga agtgcttgag gtcggcgccc gggcagaacg agctcccggc
     4381 gccggtcagc acgaggacgc gcaccgacgc gtcggcgccg gcagccacga gggcgtcgtg
     4441 ggcctcccgg accatccggt tggtcatggc gttcatggcg tcggggcggt tgagggtgag
     4501 gcgagccagc ccggccgcgg cctcgtagtg aaccgtctcc ggctcaggca tcgccgagcc
     4561 gggggaacac cgggggacgc ctctcgatca gggcggccac gccctccttg gcgtcgacgc
     4621 ggtcgagggc ggcgtactgg acctcttcgg cctcgcgcag cgccgacggg tagccgaccc
     4681 cgaggtgggc gtacacgagg cgcttggtgt cggcgagcga ggcgggagac acgttgtcgg
     4741 ccagctcgac gatgtaccgc cgggcggcgc cgataaggtc atcgggctca accacctgct
     4801 ggaccagccc gatgcggtgg gcctcggcgg cgtcgagctt gcgggacgac cacagcaggt
     4861 cgagggccgc gccggcgccg acgagtcgag gcaccatcca cgtcgtgccg tgctcggcga
     4921 tgagcccccg cctggagaag accgagacga acgacccgcg cgacgacgcg atgcgaaggt
     4981 cgctcatcac ggctagcacg aacccgccgc cggccgccac cccgttgacg gcggagacga
     5041 tcggcttggg cacctcgagc agccaggtga agattccggg caccgcgtcg gtccgcatcc
     5101 gacccgcgga gccggccgtc gtcgtctgct ggagcgccga ggagtccagg cccgagcaga
     5161 agccccgtcc cgtgccggtg accacgatgc cgaccacccg cgggtcggtc gcggcggcgt
     5221 cgaccgcctc gcggaaggcg cccagcatcg ggaacgtgag ggcgttcagc gtctcggggc
     5281 ggttcagggt gatcaacgcg accgggtcgg cgacctcgac gaggatctcc gggacgctca
     5341 tcggcgaccc gcccagcacg gttcacgcct tgccaccctt gccatgtgct ccccctccac
     5401 cgtctatcga aagatggcct tgcgcttccc gaggaaggcg gccacggcct ccttgtgctc
     5461 cggcgtggcg tacgcctggc ggagcagcgc cacctcgcgg ctctgcacga gcttgtgatc
     5521 ggtctccccc gcgttctgcg tcagcagctg cttgatccac ctgagctgcg gcgtcgggtt
     5581 ctcggcgtag gtccgggccc gctccatggc gacgtcgagc acggcgtcgt ccggtgcgac
     5641 ctcgtcggcc aggccgatgg ccgcggcttc cgtgccgagg accgtccgcc cggagagcat
     5701 gaggtccgac gccgcccccc acccgcaccg ctgcggcagc aacgagctgc tggccagctc
     5761 cggcaccagg cccatcttga cgaaccggag gctgagcttc gccgactcgg ccgccacgat
     5821 gcggtcgaag ggaaggatca tcgtcagccc cacccctatg gccggcccgt tcacggccgc
     5881 cacgatgggc ttggtcgatc ggaccagctc gacccagtcg cgccggcgcg gcggggcggc
     5941 cggcgcagcc gcaggggccg ccccggacag cagctgggtc tcgaacacct cgctcacgtc
     6001 cgcgcccgcg caaaacccgc ggcccgcgcc cgtcacgacg accgcgccga cgccggggtc
     6061 gctgtccgcg gcctcgatcg catcgccgag ctccgcgctc atgcggggcg tccacgcgtt
     6121 gagccggtcg ggacggttca gcgtgagcag cacgatctcg ccgcgctgct cggtgaggat
     6181 ctgctcgtag gtcacaccgg caccctaccc ctcccgagga aacgccgttc ggggcgcggc
     6241 ccggcgccgc cgggaccctc agttgttgcc gctgtcctcc gactcggact cggcaccctc
     6301 cggcgacccg gcctgcgtgg ccgggagctg ctgctgcatg tagccgatct tggcggcgag
     6361 cgcgttgtgg ctgtccgtgg cggcgtgcag gtcgcgctgc aggtcggcga gatcggcctg
     6421 cagcttgtcg ggctggtggt ccggctggtt ctggttgttc cattcgagca actggtcgag
     6481 gcactggacg gcctggttca gcagctggat cacgtactgc aggctctggt cgacgctctg
     6541 cagcccctcg agtgtgtcgg acatctcgcc ccctctgacg tggtgaccgc gagtgtaaac
     6601 cgtcggccca cgcctcgacc taccacgggc cgccggcacc gcataggcgt agcatcggcc
     6661 ggcaccacac gcgaggaggg ggtcatcacg atgaaggctg cttgcatgga cgatggcgtc
     6721 ctggagatcc gcgaggagcc ggtcccggat ccgggcgcgg aggaggcgct ggtgcgcatc
     6781 agcacggccg gcgtctgcca ctccgatctc cacctggccc ggggcgactg ggccggcgtg
     6841 cgcacgccac ggctcggcca cgaggccatc ggcgtcgtcg aagccctcgg gccgggcggg
     6901 gagcgttacg ctcaagtcgg cgaccgggtc atcctggggc tcgggggcag cggcggcgga
     6961 tactggtgcg gcgcttgcga gtactgcctg cgaggccagc cccgccactg cgcccagacc
     7021 aagggcatcc tcggcacgtt cgccgagtac ttcaccgtgt ggtccaaatc gctggtcaaa
     7081 ctgcccgaca gtgtgggcga ccaggaggca ccgctcgcct gtggcgggct caccgcgtac
     7141 ggcgcggtga agaagctgct caagcaccag atcctgcccg gccggcccgt ggccgtgatc
     7201 ggcgcggccg gcggcctcgg ccactatgcc gtccagatcg ccagcgcctt cggctaccag
     7261 gtggtcggca tcgacatcgg cgaggaccgc ctccagttcg tgaggtccct cggagcccac
     7321 cttgcggtcg acgcggccga ggcggccgag gtggtgcagc agcagctcgg cggggtggac
     7381 gcggccctgg tgttctcctc caagctggcc gggttcgacc tcgggctgaa gctgctgcgc
     7441 cgaggcggcc tgttcgtggc ggtcggcatc cccgccagca gcgagggaaa cctccagctc
     7501 aacccgttcg agctcttcct gcgtgaaccg acgctgatct actccgccgt cggcaccgtg
     7561 caggacatgc gcgagctggt ggacctggcc gcggcgggac gggtcaggag ccacgtctcg
     7621 cgggtgggcg cgctctcaga gctgcccgcc atcttcgacg agctccaggc cggcaagtac
     7681 ctgggccggg ccgtgatcaa cga
//
LOCUS       DASWGG010000200         4807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2772135, whole genome shotgun sequence.
ACCESSION   DASWGG010000200 DASWGG010000000
VERSION     DASWGG010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2772135"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VF954_03620"
     CDS             <1..1093
                     /locus_tag="VF954_03620"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX9530202.1"
                     /translation="HAVVPLATQAVPAVAPTTVQPALPATATVPATAADDERSGGGPP
                     RGNAIWWASLVLLAVVLAGVLYLLGRSTGMFGGTGGTPTGPLVPDVRNQVALIGTQVL
                     KDNGYQVAVSYQPSATVQPGFVIDESPEAKTHAAKGTTVTLVVSQAPNVAVPDETGKD
                     ANQANQELTAAGFQVAQTGAPSATVASGKVVSQTPAANTQAAKGSTVNLVVSTGKGQV
                     VVPDVRGQTATDAANALGQAGLVVGTQTQAPSDTVAQGSVIDTNPSQGTRVQGGSKVS
                     LTVSSGPNSVAVPDVIGETQDQATSDLQSAGFKVAVHQSADANPSDSGRVLDQKPTSG
                     NKAPRGSTVTITVATSPVSTSGPTTSTTQ"
     gene            complement(1172..1756)
                     /locus_tag="VF954_03625"
     CDS             complement(1172..1756)
                     /locus_tag="VF954_03625"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX9530203.1"
                     /translation="MTGPVVVIDNYDSFTYNLVQYLGELGAQPVVHRNDAVTVDDVAA
                     MRPAAIVVSPGPGRPDDAGISKAVIARLGATVPVLGVCLGHQCIGEVFGGQVVAAPRL
                     MHGKTSMIHHRGGGVLAGLPDPFEATRYHSLVVSSDGLPADLEITAEADDGVIMGLRH
                     RSLPIEGVQFHPESILTQAGKPLLSNFLALAAVA"
     gene            complement(1767..2474)
                     /locus_tag="VF954_03630"
     CDS             complement(1767..2474)
                     /locus_tag="VF954_03630"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX9530204.1"
                     /translation="MIRRRWRSALACVAMGFLVVVAATARRADPQSRLPQQFQLAGLI
                     QRQQGETAVLSRQVGELERQVAGLRAAGTTRAADQASIEAALADAGLRAGTVAVGGPG
                     LRVILDDSSVRRAPAGGNLDDLVIHSQDVQAVVNALWRAGAEAIAINDQRLVGTSAVL
                     CVGNTLLLNGTVHSPPYAVLALGAARERFESDDLVKILHTDASAFGLRFSVSHEDNLA
                     LPAFAGATQLRTARVGG"
     gene            2508..2819
                     /locus_tag="VF954_03635"
     CDS             2508..2819
                     /locus_tag="VF954_03635"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HEX9530205.1"
                     /translation="MAERRIRTKGRTAPSGRVTPKATPSPSGRYTPPIPRDQKVSPRW
                     VPVLILTLLLLGALVIVTNYLGLLPVPTGHGFKFRASNWYLLLGLGLITSGFITATTW
                     R"
     gene            complement(2924..3175)
                     /locus_tag="VF954_03640"
     CDS             complement(2924..3175)
                     /locus_tag="VF954_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530206.1"
                     /translation="MVLRLVVSAVVFLVVVRAGIAVLRALARPVPPPLPPGELRRVNM
                     RYRCTICGTEVKMTAAADEEPAPPRHCQEDMQLVAPAWE"
     gene            complement(3262..4455)
                     /locus_tag="VF954_03645"
     CDS             complement(3262..4455)
                     /locus_tag="VF954_03645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9530207.1"
                     /translation="MPNAVIVDVVRTAGGKRNGKLSGWHPADLAAECLKALAERNHLD
                     PELVDDVIMGCVSQVGAQAANVGRNAVLAAGWPESVPATTVDRQCGSSQQSAHFAAQG
                     VMAGAYDIVVAAGVEIMSLVPMGSSMVPSAGQPFGPGVMARYAPVGGLVPQGISAEMI
                     ADKWALSRQDLDGFGARSQQSAERATAEGRFEREIVPVPARTRDKETGQIIESSEMVT
                     ADEGIRPGTTLETLGNLKPAFKPDGKVTAGNSSQITDGASAALIMSEERATALGLTPR
                     ARFHAFSVVGVDPVTMLTGPIPATAKVLERAKMTLDQIDLVEINEAFASVVLAWEKEH
                     HPDMARVNVNGGAIALGHPLGCSGTKLLATLLNELERTGGRWGLETMCEGGGLANGTI
                     IERLG"
BASE COUNT          693 a         1755 c         1732 g          627 t
ORIGIN      
        1 ccacgccgtg gtccccctcg cgacccaggc cgtgcccgcg gtggcgccca cgacggtgca
       61 gccggcgctg cccgcgaccg cgaccgtgcc cgccaccgcc gccgacgacg agcgatccgg
      121 cggcggtccc ccgaggggca acgccatctg gtgggccagc ctggtgctgc tcgccgtggt
      181 gctcgccggg gtgctgtacc tgctgggccg cagcacgggg atgttcggcg ggaccggtgg
      241 gacgccgacc gggccgctgg tcccggacgt gcgcaaccag gtcgccctca tcggcaccca
      301 ggtcctcaag gacaacggct accaggtggc ggtgagctac cagcccagcg ccaccgtaca
      361 gcccggcttc gtcatcgacg agagccccga ggccaagacg cacgcggcca agggcacgac
      421 cgtcaccctc gtcgtcagcc aggcgcccaa cgtcgcggtc cccgacgaga ccggcaagga
      481 cgccaaccag gccaaccagg agctgaccgc ggcgggcttc caggtcgccc agacgggcgc
      541 gccatctgcg acggtggcat cgggcaaggt ggtcagccag accccggccg ccaacaccca
      601 ggcggccaag ggctcgacgg tcaacctggt ggtctccacc ggcaagggcc aggtggtggt
      661 ccccgacgtc cggggccaga cggcgacgga cgcggccaac gccctcggtc aggccggcct
      721 ggtcgtgggc acccagaccc aggccccgtc ggacaccgtg gcccagggca gcgtcatcga
      781 caccaacccg tcgcagggca caagggtgca gggcggcagc aaggtcagcc tcaccgtgtc
      841 cagcggcccc aacagcgtgg ccgtccccga cgtgatcggt gagacccagg accaggccac
      901 ctccgacctc cagagcgcgg gcttcaaggt cgccgtccac cagtccgccg acgccaaccc
      961 ctcggactcg ggaagggtcc tcgaccagaa gccgacctcg ggcaacaagg cgccccgggg
     1021 ctcgacggtg acgatcacgg tcgcgaccag cccggtcagc acctcgggcc ccacgacgag
     1081 cacgactcaa taagcccgac cggggcccca accccggcct cctccgcacg gcgggggccc
     1141 cagccccgcc ggcggccgtg ctctcccggg cctaggcgac ggcggccagg gccaggaagt
     1201 tcgacagcag gggcttgccc gcttgggtga ggatgctctc ggggtggaac tgcacgccct
     1261 cgatgggcag ggaccggtgg cgcagcccca tgatcacccc gtcgtcggcc tcggccgtga
     1321 tctccaggtc ggcgggcagg ccgtcggagg acacgaccag ggagtggtag cgggtggcct
     1381 cgaaggggtc ggggaggccg gccagcacgc cgccgccgcg gtggtggatc atggacgtct
     1441 tgccgtgcat caggcggggc gcggccacga cctggccgcc gaacacctcg ccgatgcact
     1501 ggtggccgag gcagacgccg agcaccggga cggtcgcccc gaggcgggcg atgacggcct
     1561 tgctgatgcc ggcgtcgtcg ggccggcccg ggcccggtga gacgacgatc gccgccggtc
     1621 gcatggcggc cacgtcgtcg accgtcacgg cgtcgttgcg gtggaccacc ggctgggcgc
     1681 cgagctcgcc caggtactgg accaggttgt aggtgaagga gtcatagttg tcgatcacca
     1741 ccaccgggcc ggtcacggcc tcgaggctac ccgccgaccc gggccgtcct gagctgggtc
     1801 gcgcccgcga aggccggcag ggccaggttg tcctcgtggg acacggagaa gcgcaggccg
     1861 aacgccgacg cgtcggtgtg gaggatcttc accaggtcgt cggactcgaa ccgctcacgg
     1921 gccgcgccca gggccagcac cgcgtagggc ggcgaatgca ccgtgccgtt gagcagcagg
     1981 gtgttgccca cgcagagcac cgcgctcgtg cccaccaggc gctggtcgtt gatggcgatg
     2041 gcctcggcgc ccgcccgcca cagcgcgttg accaccgcct ggacgtcctg ggagtggatg
     2101 acgaggtcgt cgaggttgcc gccggcgggc gcccggcgga ccgacgagtc gtcgaggatc
     2161 acccgcaggc cggggccgcc gaccgcgacc gtcccggccc gcaggccggc gtcggcgagg
     2221 gccgcctcga tcgaggcctg atcggcggcg cgcgtggtgc cggcggcgcg gagaccggcg
     2281 acctggcgct ccagctcgcc cacctgccgg ctgagcaccg ccgtctcgcc ctgctggcgc
     2341 tggatgagcc cggccagctg gaactgctgc gggaggcggg actgcggatc ggcccggcgg
     2401 gcggtcgccg ccaccaccac caggaaaccc atggccacgc acgccagcgc gctgcgccac
     2461 cggcgccgga tcacggggcc ctccccggct cgctatcctc gggcgccatg gccgaacgcc
     2521 ggatccgcac caaagggcgg acggccccca gtggccgggt cacgcccaag gcgacgcctt
     2581 cgccgagcgg caggtacacc ccgcccatcc cgcgtgacca gaaggtcagc ccccgctggg
     2641 tgccggttct gatcctgacg ttgctgctgc tcggcgcgct ggtgatcgtg accaactacc
     2701 tcgggctact gccggtgccg accggccacg ggttcaagtt ccgggcttcc aactggtacc
     2761 tcctgctcgg cctgggcctc atcaccagcg ggttcatcac cgccaccacc tggcgctgat
     2821 cgcgatgtcg tcccggtgga tggggcgcgg acgataaacg caagattgct gccaagttac
     2881 atccatgtga cactccccaa cattgtccac aatgtgggga caactactcc cacgccgggg
     2941 ccacgagctg catgtcctcc tggcagtggc ggggcggggc gggctcctcg tcggcggcag
     3001 cggtcatctt gacctcggtc ccgcagatgg tgcagcggta gcgcatgttc accctgcgca
     3061 gctcgcccgg cggcaggggc ggcgggacgg gccgggccag ggcccgcagc acggcgatcc
     3121 cggcccggac gaccaccagg aagaccaccg cgctgacgac cagccgcaac accacggcgc
     3181 gccatcctac ggcggcggct ccggaagggc gcagccggcc gcggccggga gggtcgccct
     3241 ttctcccgag cctctagagc ctcagcccag gcgctcgatg atggtgccgt tggccaggcc
     3301 gccgccctcg cacatggtct cgaggcccca ccgcccgccg gtgcgctcca gctcgttgag
     3361 cagggtggcg agcagcttgg tgcccgagca gccgagcggg tggcccaggg cgatggcgcc
     3421 gccgttgacg ttgacccggg ccatgtcggg gtggtgctcc ttctcccacg ccagcacgac
     3481 cgaggcgaag gcctcgttga tctcgaccag gtcgatctgg tcgagcgtca tcttggcccg
     3541 ctccagcacc ttggcggtgg ccgggatggg gccggtgagc atggtgaccg ggtccacgcc
     3601 gaccaccgag aaggcgtgga aacgggcccg gggcgtgagg ccgagcgccg tggcccgctc
     3661 ctcgctcatg atcagggccg ccgaggcgcc gtcggtgatc tgcgaggagt tgccggcggt
     3721 gaccttgccg tcgggcttga aggcgggctt caggttgccc agcgtctcca gggtcgtgcc
     3781 tgggcggatg ccctcgtccg cggtgaccat ctcgctggac tcgatgatct ggccggtctc
     3841 cttgtccctg gtccgagccg gcacggggac gatctcccgc tcgaagcggc cctcggcggt
     3901 cgcccgctcg gcggactgct ggctccgggc cccgaacccg tcgaggtcct ggcgggacag
     3961 cgcccacttg tcggcgatca tctcggccga gatcccctgg gggaccaggc cgcccaccgg
     4021 ggcgtagcgc gccatgacgc cggggccgaa gggctggccc gcgctcggca ccatggagga
     4081 gcccatgggc accaggctca tgatctcgac gccggccgcg accacgatgt cgtaggcgcc
     4141 cgccatgacc ccctgggcgg cgaaatgggc cgactgctga gacgagccgc actgccggtc
     4201 gacggtggtg gccggcaccg actccggcca accggccgcc agcacggcgt tgcggcccac
     4261 gttggcggcc tgggcgccga cctgggagac gcagcccatg atcacgtcgt cgaccagctc
     4321 ggggtcgagg tggttgcgct cggccagcgc cttcaggcac tcggcggcca ggtcggcggg
     4381 atgccagccc gacagcttgc cgttgcgctt accccctgcg gtgcggacca cgtcgacgat
     4441 cacggcgttg ggcattggac gactccttcg ctttggtgag agctgctttg gtgagaacta
     4501 gaccggccgg tcaagtttgg tgcccgccga ccggcagggt caatctaccg gtggccgcca
     4561 ggcgccgggg ccgcccctcc gggccgggaa ccgggccggg aaccgggccg ggcacccacg
     4621 gtttgatcac ctacctaccg gtcggtaggg tgacgggatg caggacgtgg ccctcgtagc
     4681 cgagctcgaa cctgtggccg gtcagctgct ggaccgccac ctgacgacga cgaaggagtg
     4741 gttcccccac gagctcgtcc cctggtcgct tggacgggac ttcgagcccg gctggtcgtg
     4801 ggagccg
//
LOCUS       DASWGG010000201         4777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_841901, whole genome shotgun sequence.
ACCESSION   DASWGG010000201 DASWGG010000000
VERSION     DASWGG010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841901"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..479)
                     /locus_tag="VF954_03650"
     CDS             complement(15..479)
                     /locus_tag="VF954_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530208.1"
                     /translation="MHLVRGGSARSPALLGAALASAALGVCLWLPWLRTGGTRRSAYG
                     LLTALRAAGLLSSGARRAFFAALAVAPGLTGAAWVLAFARRRRLSGLATAAVGTITAA
                     AGAAVRHYSVRGAQSGTVWAEVVGVVTLAMGVALTGWRAPPGRKGTRDDRRR"
     gene            608..1723
                     /locus_tag="VF954_03655"
     CDS             608..1723
                     /locus_tag="VF954_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530209.1"
                     /translation="MAQVRVLIGTRKGAFILTSDGARAEWKVDGPHFGGWEIYHVKGS
                     PADPDRLYASQSTGWFGQIVQRSDDGGATWTAVGNQFPYEGVPGTHQWYDGTPHPWEF
                     ARVWHFEPSATDPDTVYAGVEDAALFRSTDGGLAWQELPGLRGHGSGPSWQPGAGGMC
                     LHSIIIDPVDAARMYVAISAAGAFRTDDGGKSWRPVNRGLRSEGIPDPTAEVGHCVHH
                     LAMHPSRPDVLFMQKHWDVMRSDDGGGSWREISGDLPTDFGFAIDVHAHEPDTIYVVP
                     ITSDSLHFPPDGSLRVYRSRSGGDEWQPLTAGLPPRHCYVNVLRDAMAVDSLDPCGVY
                     FGTTGGQVYASPDQGDTWAPIVRDLPAVLSVEVQTLP"
     gene            1720..2016
                     /locus_tag="VF954_03660"
     CDS             1720..2016
                     /locus_tag="VF954_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX9530210.1"
                     /translation="MIRVVLPAHLRTLAGVQGEVVLAVPGTASQRTLLDALEARFPML
                     QGTIRDPVSLRRRGFVRFFACEEDLSHEAADAPLPQAVVSGAEPFIVLGAMAGG"
     gene            complement(2013..2432)
                     /locus_tag="VF954_03665"
     CDS             complement(2013..2432)
                     /locus_tag="VF954_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530211.1"
                     /translation="MARKERWKQEPDGHDFPAASDYLTLLLGEPAARAVVERLRAASV
                     THRKAKDLLRASGLELLPADNVHVAKDLAKVKSREKLSPVLVVRGDLASGRPLTVADG
                     YHRICASHHLDEDADIPCRLVDAPTGAAQGTAPRKSN"
     gene            2526..4067
                     /locus_tag="VF954_03670"
     CDS             2526..4067
                     /locus_tag="VF954_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9530212.1"
                     /translation="MPGGLTWRATEVDGRPARYGIGGAGPPVVFLHGWALGSQTYRRS
                     LRRLIDRGCQVVAPALPGLGGTADLPADRVSIAGYAAWVDTFMAAVGIDQPAMVIGHS
                     FGGGVAAKLAHTFPDRVGHLVLLNSVGGVTDRPMWLWGLQFARELLPDRQGVGTILAM
                     RRDLVNNLLSNPGGLLRVGQLARTADLRAELAELRQRGLPVLALTTAEDLVIPRSAFA
                     ALCRAIGTDGQVLAGRHSWLLSDPDTFGEVLGNELEVQVAEHQARGAASRVVEVVDAL
                     RSTRVPSRTARSLLAQAPPLWLMSAPAIVLAGDLVLCHPKLGSGEVRAIARPMSEPGA
                     TRLTVVAPDRPGLLADTAAAVSAHGLSITEASASTWASRHLALHALTVVGAAGFAEPD
                     WDRLGADLRDPSRLRPRPLHPSRVRARVSAYDAATDRKLVRVSAPDQAGLLWAVCDWF
                     AGRMVSIECLDASTVDGVAHDVFLVRGHFDSDDLVADLSRGRRGDRSAPALRRGRTAT
                     RPIAS"
     gene            complement(4128..4430)
                     /locus_tag="VF954_03675"
     CDS             complement(4128..4430)
                     /locus_tag="VF954_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530213.1"
                     /translation="MNCGCPLDWEASLRSRCTNCRAAVARAGRASTSDGQILPVLQAV
                     PGGSEPGDPRRALVGVASVSRLADRAPDLLPDGRGGWQRDQEEASLLSIVLHELAE"
     gene            complement(4517..>4777)
                     /locus_tag="VF954_03680"
     CDS             complement(4517..>4777)
                     /locus_tag="VF954_03680"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9530214.1"
                     /translation="VTLRAVATNRGIDVTGTVRAEGDLDFRGTLAVDRDAPVGFQAIR
                     LAFDLESSAPPEELQRLVATTERYCVVLQTLSRQPDLSVVVT"
BASE COUNT          613 a         1783 c         1694 g          687 t
ORIGIN      
        1 ccccgtcgcc ccggtcagcg tcgtcggtca tctcgcgtcc ccttccgccc gggcggagcc
       61 ctccaccccg tcagcgccac gcccatcgct agggtcacga cgcccaccac ctcggcccac
      121 acggtacccg attgcgctcc ccggacggaa taatggcgga ccgcagcccc ggctgcggcc
      181 gtgatggtcc ccaccgcggc cgtggccagc ccactcagcc tgcgccggcg ggcgaaggcc
      241 agcacccagg ccgcaccggt gaggcccggc gccaccgcca gcgcggcgaa gaaggcgcgc
      301 cgggcacccg aggacaacag gccggcggcc cggagggcgg tgagaagccc gtaggccgat
      361 cgacgagtgc cgccggttcg cagccacggc agccacaggc acacccccag agcggcggat
      421 gccagcgccg ctcccaggag ggcgggcgag cgcgccgacc cgcccctcac gaggtgcacg
      481 tcacgaaggc gcacagtttc gaccgtcccg atggtaccgg gggcgaaggc gaccgatcag
      541 tctggggtcg ggtccgcgtc atatggtggg aaccgcggcc gcgggccgcg caaggaggtc
      601 atccggcatg gctcaggtga gggtgttgat cggcacacgc aagggggcct tcatcctcac
      661 gtcggacggc gcccgggccg agtggaaggt cgacgggccc catttcgggg ggtgggagat
      721 ctaccacgtc aaaggatcgc ccgccgatcc cgacaggctg tacgcgtccc agtccacggg
      781 ctggttcggc cagatcgtcc agcgctccga cgacggcggc gccacatgga cggcggtcgg
      841 aaaccagttc ccgtacgagg gcgtcccggg cacgcaccag tggtacgacg ggactcccca
      901 tccctgggaa ttcgcccggg tctggcactt cgagccgtcg gccaccgacc ccgacaccgt
      961 ctacgccggg gtcgaggacg ccgcgctgtt ccggtcgacc gacggtgggc tggcctggca
     1021 ggagctgccc gggctccgcg ggcacgggtc cggtccctcc tggcagcccg gcgcgggagg
     1081 gatgtgcctc cactccatca tcatcgatcc cgtcgatgcg gcccgcatgt acgtggccat
     1141 ctcggcggcg ggcgccttcc ggaccgacga cggggggaag agctggcggc ccgtcaaccg
     1201 cggcctgcgc tcggagggca taccggaccc gaccgcggag gtcgggcatt gcgtgcacca
     1261 tctcgccatg cacccgtcgc ggcccgacgt gctgttcatg cagaagcact gggacgtgat
     1321 gcgcagcgac gacgggggcg ggtcgtggcg ggagatcagc ggtgacctgc ccaccgactt
     1381 cggcttcgcc atcgatgtcc atgcccacga gcccgacacg atctacgtgg tccccatcac
     1441 cagcgactcg ctgcacttcc cacccgacgg cagcctccgg gtctaccgga gccgcagtgg
     1501 gggcgacgag tggcagccgc tcacggccgg cctgccgccc cgtcactgct acgtcaacgt
     1561 gctgcgagac gccatggcgg tcgactccct cgacccctgc ggggtgtact tcggcacgac
     1621 cggcgggcag gtctacgcct cgcccgacca gggtgacacc tgggcgccca tcgtgcgcga
     1681 cctgcccgcc gtcctgtccg tcgaggtgca gacgctgccg tgatccgcgt ggtcctgccc
     1741 gcgcatctgc ggacgctcgc gggcgttcag ggggaggtgg tgctcgccgt cccggggacc
     1801 gcctcccagc gcaccctcct cgacgccctg gaggcgcgct ttcccatgct ccaagggacg
     1861 atccgcgacc cggtcagcct ccgccgccgg ggcttcgtgc ggttcttcgc ctgtgaggag
     1921 gacctctccc acgaggccgc ggatgccccc ctgccccagg ccgtggtgtc cggtgccgag
     1981 cccttcatcg tgctcggcgc catggccggc ggctagttcg acttgcgagg ggccgtcccc
     2041 tgcgccgccc cggttggagc gtcgaccagg cggcacggga tgtcggcatc ctcgtctagg
     2101 tggtggctgg cgcagatccg gtggtagccg tccgccaccg tgagcggccg cccgctcgcc
     2161 aggtcccctc ggacgacaag cacgggggat agcttctctc ggctcttcac cttcgccagg
     2221 tccttggcca cgtggacgtt gtccgcgggg agcagctcga ggccactcgc ccgcaggagg
     2281 tccttggcct tccggtgggt gacggacgcg gcccggaggc gctcgacgac cgcccgggcg
     2341 gcaggctcgc cgaggagcag ggtgaggtag tccgacgcgg cgggaaagtc gtggccgtcg
     2401 ggctcctgct tccaacgctc ttttctggcc atgtcccctc ctggtcgggc gtttcgtgat
     2461 ggtaacggcc gggagttcat cggctggaaa cacgcgtgcc ggtgcccggg cgtaccgtcg
     2521 cgcaggtgcc cggtggcctg acgtggcgtg cgacggaggt cgacggccgc cccgcgaggt
     2581 acgggatcgg cggggcagga cctcccgtgg tgttccttca cggctgggcc ctcgggagcc
     2641 agacctaccg ccggtccctg cggcggctca tcgaccgggg ctgtcaggtg gtggctccgg
     2701 ccctgcccgg actgggcggc acggccgatc tgcccgccga ccgggtctcg atcgcgggct
     2761 acgccgcctg ggtcgacacg ttcatggccg cggtcggcat cgatcaaccg gcgatggtca
     2821 tcgggcactc gttcgggggc ggcgtggcgg ccaagctggc ccacaccttc cccgaccggg
     2881 tcggccacct ggtgctgctc aactcggtcg gcggcgtcac cgaccggccc atgtggctct
     2941 ggggcctcca gttcgcgcga gagctgctgc cggatcgaca gggcgtgggg acgatcctgg
     3001 ccatgcgtcg ggacctggtc aacaacctgc tcagcaaccc gggcgggctc ctgcgggtcg
     3061 ggcagctggc ccgcaccgcg gacctgcgcg ccgagctggc cgagctccgc cagcggggcc
     3121 tacccgtgct cgcgctgacg acagcggagg acctggtgat cccgcggtcg gccttcgcag
     3181 ctctgtgccg agcgatcggt accgacgggc aggtgctcgc cggcaggcat tcctggctgc
     3241 tgtccgatcc cgacaccttc ggggaggtgc tcggcaacga gctcgaggtc caggtcgccg
     3301 agcaccaggc caggggggcg gccagccgag tggtggaggt cgtcgatgcc ctgcgatcca
     3361 cccgagtccc gagccggacg gcccgctccc tgctggccca ggctcccccg ctctggctca
     3421 tgagcgcgcc ggccatcgtc ctggccggcg acctggtgct gtgccatccc aagctcgggt
     3481 ccggggaggt ccgcgccatc gcccgtccga tgtccgaacc gggggcgacg aggctcaccg
     3541 tcgtcgcgcc cgaccggccc gggctcctcg ccgacacggc cgccgccgtc tcggcccacg
     3601 gtctgtccat caccgaggcg tccgcctcga cgtgggcgtc ccggcacctg gcgctgcatg
     3661 ccctcacggt tgtgggcgcg gccggtttcg ccgagcctga ctgggaccgg ctgggggcag
     3721 acctccggga tcccagccgc ctgcggcccc gaccgttgca tccgtctcgg gtgcgggccc
     3781 gggtctccgc gtacgatgcg gccaccgaca ggaagctcgt gcgcgtcagc gcacccgatc
     3841 aggccggtct gctctgggcc gtctgtgact ggttcgccgg ccggatggtg agcatcgaat
     3901 gcctggatgc ctcgaccgtc gacggcgtgg cccacgacgt cttcctcgtg agggggcact
     3961 tcgacagcga cgacctggtg gccgacttgt cccgagggcg ccgcggcgac cgttcggctc
     4021 ccgcgcttcg tcgcgggcgc accgccacac ggcccatcgc ctcgtaaccg ggtccccatg
     4081 agggccggcc ggcgcggcgg ccccgcgccc cggcgcggca gccggcgcta ctcggccagc
     4141 tcgtgcagaa cgatcgacag cagcgaggcc tcctcctggt cgcgctgcca accgccacgc
     4201 ccgtcgggca ggaggtctgg cgcgcggtcg gccagacggg acacgctggc cacacccacc
     4261 agggcccggc gagggtcgcc gggttcgctc ccgcctggaa cggcctggag cacggggagg
     4321 atctgcccgt cggaggtgct cgcccggccg gcgcgggcca ccgcggcccg gcagttcgtg
     4381 caccggctcc gcaggctggc ctcccagtcc agcgggcacc cacagttcaa acagcgcatc
     4441 gtctctgccc tctctcgccc ccgaagcggc cttgccctgt tcatcgcgcc ggccgccgtc
     4501 tccgttacgg cgccgctcag gtcacgacga ccgacaggtc tggctgtcgg gacaaggtct
     4561 ggagcacgac gcagtacctc tccgtcgtgg ccaccagccg ctggagctct tcggggggag
     4621 cgctcgactc gaggtcgaac gccagtcgta tcgcctggaa gcccacgggc gcgtcccggt
     4681 cgaccgccag ggtgccccgg aagtcgagat cgccctcggc ccgcaccgtt cccgtcacgt
     4741 cgatgccccg gttggtggcc acggcccgca gcgtcac
//
LOCUS       DASWGG010000202         5792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1814168, whole genome shotgun sequence.
ACCESSION   DASWGG010000202 DASWGG010000000
VERSION     DASWGG010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814168"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VF954_03685"
     CDS             <1..580
                     /locus_tag="VF954_03685"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX9530215.1"
                     /translation="IPPLLVLEEIGLFTGATEWVELAVTQLRRRRDELVRPGNAAERL
                     AARIGRTIPLVHSSGAVGTCAAMRWKAQLNENAKSPAFSAIYPELCHNEIQGWGQHGD
                     VTRQLITIVNLRHDAEHPQVSRRFELVGELVREVVHGIEEVHAEGEGDLAQLLDLVLF
                     GDFVSLHAAAREGIDPGPIPTLTDLKRRLAGT"
     gene            634..828
                     /locus_tag="VF954_03690"
     CDS             634..828
                     /locus_tag="VF954_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530216.1"
                     /translation="MPPDHPTATAAAIPAGMIESLLADCGVRQEAVARARARLAGASP
                     CPAEELAATLVDCMVAGRLP"
     gene            1364..2065
                     /locus_tag="VF954_03695"
     CDS             1364..2065
                     /locus_tag="VF954_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9530217.1"
                     /translation="MSDQHSSVSDEVIRVLIADDQALFRRGLYVVLGTEDGIEVVGEA
                     ENGEEAVGKAEDLAPDVVLMDVRMPKVNGIEAARAIRAMAPSTRILMLTVSDEEEDLY
                     EAIKAGANGYLLKEISVEEVAEAIRAVVQGQSLISPSMASKLLSEFNSLVRRAEEKPQ
                     FPAPALTSRELEVLKLVAKGMSNREIADELFISENTVKNHVRNILEKLHLHSRMQAVM
                     YAVREQLIDPDDKER"
     gene            complement(2078..3583)
                     /locus_tag="VF954_03700"
     CDS             complement(2078..3583)
                     /locus_tag="VF954_03700"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEX9530218.1"
                     /translation="MDDEPWWQRAYRHPDAPPVVVLAVMMAAYVAVFGTLTWRQQSHF
                     GTFGFDMGIHDQGIWLLSRFRDPYDTVVGRNYLGHHVNLVSLLFVPAYWLGAGPHFLY
                     LAETLALAVGALPVWLLARDRLGNGWTALPLAAAYLLYPSIEWINWWHFHPDALMVTP
                     LLLAWWLAARARWRWFAAAVAVLLVCKEDAALAVAALGAVLVIRRVRERGPRAWKAGA
                     VTAAVGAGWFLVATKALIPLLNGGQRAFYEDMFPAFGHGLGTIAFNFVAHPSRLWHLA
                     TKPDRLRYYRQLLAPVGAVALGAPLPLLIAAPQLAVNVTASLGYTHDIRYHYSSIVAA
                     TVLIATVEAIGRWRHRPAVVRALVGLVAATALAANVDLSPSPLGTAYHSGIWAPASPR
                     IAALDAAVGEVPPGAGVSASYTLVPHLTHRATAYEFPNPWVLSNWLDRRRRPDPAKVQ
                     FLVLDRTLNPDQAGLIDELTGATGPFEIVYDRDQVIVARRVVPPSGANGGQ"
     gene            3625..>5792
                     /gene="secA"
                     /locus_tag="VF954_03705"
     CDS             3625..>5792
                     /gene="secA"
                     /locus_tag="VF954_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX9530219.1"
                     /translation="MSILSKVLRAGEGKKLRRLQEIVPDINTLEPAFEALSDDALRHK
                     TVEFRERLANGEDLDDLLIEAFATVREGARRVIGQRHFDVQLMGGMALHFGWIAEMKT
                     GEGKTLVSTLPVYLNGLTGQGVHLVTVNDYLARFHAEWMGQIYRWLGISVGLVVPDVD
                     DWAAKREAYHCDVTYGTNTEFGFDYLRDNMARSLDAMVQRKHVYAIVDEVDSILIDEA
                     RTPLIISGPASESARLYYQFAGVVRGLGREADYEVDEEKRTVVPTEEGIDKVERQLGV
                     DNMYDLVSVNYVHQLTQALRAKELYHRDKDYIVQGGEVKIVDEFTGRILEGRRWSDGL
                     HQAVEAKERVKIKEENHTWATITLQNYFRLYHKLAGMTGTAETEASEFANTYNLPVVP
                     IPTNRPMVRDDKADLIYKSEEAKFEAVVGDLVERYETGQPVLVGTASVERSEHLSRLL
                     DKKGVPHAVLNAKQHTREAHIVAQAGRLHGVTVATNMAGRGVDILLGGHPEGLARNDV
                     LAEGLDPDAEEGRARLAELLPKYDAECQAEGDQVRGLGGLYVLGSERHESRRIDNQLR
                     GRSGRQGDLGESRFYLSLEDELMRLFATGAMNWVMGKALPDDVPIEARMVTKAIERAQ
                     NTVEARNAEIRKDVLKYDEVMNEQRKVIYARRMQVLEGEDLRDNTLEVLEDTIDAVVG
                     DHCPNDFPEDWDLDELLAQVNLIYPTKFTAGELRTAATAA"
BASE COUNT          972 a         1974 c         2094 g          752 t
ORIGIN      
        1 catcccgccc ctgctcgtgc tggaggagat cgggctgttc accggcgcga ccgagtgggt
       61 ggagctggcg gtcacccagc tccggcgccg ccgggacgag ctggtcaggc cgggcaacgc
      121 agccgagcgg ctggcggcgc ggatcgggcg caccatcccg ctcgtgcact cgtcgggcgc
      181 cgtggggacg tgcgcggcga tgcgctggaa ggcccagctc aacgagaacg ccaagtcccc
      241 tgcgttctcg gccatctacc cggagctgtg ccacaacgag atccagggct ggggccagca
      301 cggcgacgtc acccggcagc tgatcaccat cgtcaacctc cgccacgacg ccgagcaccc
      361 ccaggtcagc cggcgcttcg agctggtggg cgagctggta cgcgaggtcg tccacggaat
      421 cgaggaggtc cacgccgagg gggagggcga tctggcccag ctgctggacc tggtgctgtt
      481 cggcgacttc gtctcgctgc acgccgcggc gcgagaaggc atcgaccccg gtccgatccc
      541 caccctcacc gatctcaagc gacgccttgc cggcacgtga cgtggtggtt ttcgccactc
      601 aaggtggttg ttgtccggac cgacgatgca agggtgcccc ccgaccaccc cactgcgacc
      661 gccgccgcca tcccggccgg catgatcgag tcgctgctgg ctgactgcgg ggtgcgccag
      721 gaggcggtgg cgcgggctcg cgcccgcctg gccggggcct cgccctgccc ggccgaggag
      781 ctggccgcca ctctcgtgga ctgcatggtg gccggccgcc tgccgtaggg ctcacagagc
      841 ccctggccac ggggcccggg tcgctgagcc ccggggcaga cggcccatac actcggtcga
      901 tgggtggcga gcgggtctgt ggttgcaagt cgatgccagt cgcaggcgaa acgacccacg
      961 taaggcaacc tgtggttgct gagcatggtg cggcttagga gtaagccctg cccgccggcg
     1021 cgccgatccc cggtgctccg ggggagaggg agcgatgcgg gagcatcccg gagcgttggg
     1081 cctagagccg ccgcccgggc ccgtcacctc cccagcaggc gagtgtgggg ccaaagacca
     1141 ggtcagccgc tcgccaccct tttcactgct gatcatactg ggggagggcc cgtgtcggta
     1201 cactttcagc agtccgggct agacacgccg gcaatggctc ggacggacgg gaacatgacc
     1261 aggcctcact cacccaggcg cagcagttgc ctaccgatgt gctaactctc tcgggccaga
     1321 cccggcgccg acctgaccgc actccagcca ccagggggac gccgtgagcg accaacactc
     1381 gagcgtgagc gacgaggtca ttcgcgtcct gatcgcagat gaccaggcgc tgttccgccg
     1441 cggcctgtac gtggtgctcg ggaccgagga cggcatcgag gtggtcggcg aggccgagaa
     1501 cggcgaagag gcggtcggca aggccgagga cctcgccccc gacgtcgtgc tgatggacgt
     1561 gcggatgccc aaggtcaacg gcatcgaagc ggcccgggcc atccgggcca tggcgccgag
     1621 cacccgcatc ctcatgctga ccgtgagcga cgaggaggag gacctctacg aggcgatcaa
     1681 ggccggggcc aacgggtacc tgctgaagga gatctcggtc gaggaggtgg ccgaggccat
     1741 ccgggcggtg gtccagggcc agagcctgat ctcaccgtcg atggcgtcca agctgctgtc
     1801 ggagttcaac tcgctcgtgc gccgggcgga ggagaagccg cagttccctg cgccggccct
     1861 cacgagccgc gagctggagg tgctcaagct ggtcgccaag ggcatgagca accgggagat
     1921 cgccgacgag ctgttcatct cggagaacac cgtcaagaac cacgtgcgca acatcctcga
     1981 gaagctccac ctgcactccc ggatgcaggc cgtgatgtac gccgtgcggg agcagctgat
     2041 cgaccccgac gacaaggagc gctagcagct cacctgctca ctgccccccg ttggcgccgg
     2101 acggggggac gacccgccgg gccacgatga cctggtcccg gtcgtagacg atctcgaacg
     2161 ggcccgtggc gccggtgagc tcgtcgatca gtccggcctg gtcggggttg agcgtgcggt
     2221 ccagcacgag gaactggacc ttggccgggt cgggccgccg ccgccggtcg agccagttgc
     2281 tgaggaccca cgggttcggg aactcgtacg cggtggcccg gtgggtcagg tggggcacga
     2341 gcgtgtagct ggcgctgacg cccgcgccgg gcggcacctc accgacggcg gcgtcgagtg
     2401 ccgcgatccg cggggaggcc ggggcccaga ttccggagtg gtaggcggtg cccagcgggg
     2461 agggggaaag gtcgacgttg gcggcgaggg ccgtcgcggc gaccaggccg acgagcgccc
     2521 ggaccacggc cgggcggtgg cgccagcggc cgatggcctc gacggtggcg atgagcaccg
     2581 tcgcggccac gatcgagctg tagtggtacc tgatgtcgtg ggtgtagccg agcgaggcgg
     2641 tcacgttgac ggccagctgc ggggcggcga tgaggagggg gaggggggcg cccagggcca
     2701 ccgcgccgac cggggctagc agctggcggt agtacctgag acggtcgggc ttggtggcca
     2761 ggtgccagag ccggctcggg tgggccacga agttgaaggc gatcgtgccg agcccgtgcc
     2821 cgaaggccgg gaacatgtcc tcgtagaacg cccgctggcc gccgttgagc agcgggatga
     2881 gggccttggt ggccactagg aaccacccgg ccccgacggc cgcggtgacg gcgcccgcct
     2941 tccacgcccg cgggccgcgc tcgcgcaccc gtcggatgac gaggaccgcc ccgagggcgg
     3001 ccacggccag ggcggcgtcc tccttgcaca cgagcaggac cgccacggcg gccgcgaacc
     3061 accgccaccg cgcccgggcc gccagccacc aggccagcag cagcggcgtc accatcaggg
     3121 cgtcggggtg gaagtgccac cagttgatcc actcgatcga ggggtacagc aggtaggccg
     3181 ccgccagggg cagggcggtc cagccgttgc ccaggcggtc gcgggcgagc agccagacgg
     3241 gcagggcgcc gacggccagg gcgagggtct cggccaggta gaggaagtgg ggccccgccc
     3301 ccagccagta ggccggcacg aacagcaggc tgacgaggtt cacgtggtgg cccaggtagt
     3361 tgcggcccac gacggtgtcg taggggtccc tgaagcggga gagcagccag atgccctggt
     3421 cgtggatgcc catgtcgaac ccgaaggtcc cgaagtggct ctgctggcgc caggtcagcg
     3481 tgccgaacac cgccacgtac gccgccatca tcaccgccag gacgacgacc ggcggcgcgt
     3541 ccgggtggcg gtaggcccgc tgccaccagg gctcgtcgtc cacggcctgg gatggtaggg
     3601 ggccgccgat agactcggat gccgatgagc atcctcagca aggtcctgcg cgcgggcgag
     3661 gggaagaagc tgcgccggct ccaggagatc gtgccggaca tcaacaccct cgaacctgcc
     3721 ttcgaagccc tgtcggacga cgccctgcgg cacaagacgg tcgagttccg ggagcggctg
     3781 gccaacggcg aggacctcga cgacctgctg atcgaggcgt tcgccaccgt gcgggagggc
     3841 gcccggcggg tcatcggcca gcgccacttc gacgtccagc tgatgggcgg catggccctg
     3901 cacttcggct ggatcgcgga gatgaagacc ggtgagggca agaccctcgt gtcgaccctg
     3961 cccgtctacc tcaacggcct gaccggccag ggggtgcacc tggtcaccgt caacgactac
     4021 ctggcccgtt tccacgccga gtggatgggc cagatctacc ggtggctggg catcagcgtc
     4081 gggctggtgg tccccgacgt cgacgactgg gcggccaagc gggaggccta ccactgcgac
     4141 gtcacctacg gcaccaacac cgagttcggc ttcgactacc tccgggacaa catggcccgc
     4201 agcctcgacg ccatggtcca gcgcaagcac gtgtacgcca tcgtggacga ggtcgactcg
     4261 atcctcatcg acgaggcccg gaccccgttg atcatcagcg gcccggcgtc ggagtcggcc
     4321 cggctgtact accagttcgc cggcgtggtg cgggggctcg gccgcgaggc cgactacgag
     4381 gtcgacgagg agaagcgcac ggtcgtgccc accgaggagg gcatcgacaa ggtcgagcgc
     4441 cagctcgggg tcgacaacat gtacgacctg gtctctgtca actacgtgca ccagctcacc
     4501 caggcgctgc gcgccaagga gctgtaccac cgggacaagg actacatcgt ccagggcggc
     4561 gaggtgaaga tcgtcgacga gttcaccggc cgcatcctgg agggccggcg ctggtcggac
     4621 ggcctccacc aggcggtgga ggccaaggag cgggtcaaga tcaaggagga gaaccacacc
     4681 tgggcgacca tcaccctcca gaactacttc cggctctacc acaagctggc cggcatgacg
     4741 ggcacggcgg agaccgaggc ctcggagttc gccaacacct acaacctgcc ggtcgtgccc
     4801 atccccacca accggcccat ggtccgcgac gacaaggccg acctcatcta caagagcgag
     4861 gaggccaagt tcgaggccgt ggtgggcgac ctggtcgagc gttacgagac cgggcagccc
     4921 gtcctggtgg gcacggcgtc ggtcgagcgg tccgagcacc tgtcccggct gctggacaag
     4981 aagggggtcc cgcacgcggt gctcaacgcc aagcagcaca cccgggaggc ccacatcgtc
     5041 gctcaggccg gccgcctcca cggcgtgacg gtcgcgacca acatggccgg ccggggcgtc
     5101 gacatcctgc tgggcggcca ccccgagggc ctggcccgca acgacgtgct ggccgagggc
     5161 ctcgatcccg acgccgagga gggcagggcc cgcctggccg agctgctgcc caagtacgac
     5221 gccgagtgcc aggccgaggg cgaccaggtc cgtgggctcg gcggcctgta cgtcctcggc
     5281 agcgagcgcc acgagagccg gcgcatcgac aaccagctgc ggggccggtc cggccgccag
     5341 ggcgacctgg gcgagagccg gttctacctg tcgctcgagg acgagctcat gcgcctgttc
     5401 gccaccggcg ccatgaactg ggtgatgggc aaggccctgc ccgacgacgt gcccatcgag
     5461 gcccgcatgg tgaccaaggc gatcgagcgg gcccagaaca ccgtcgaggc ccgcaacgcc
     5521 gagatccgca aggacgtgct caagtacgac gaggtcatga acgagcagcg caaggtcatc
     5581 tacgcccgcc gcatgcaggt cctcgaaggc gaggacctcc gggacaacac cctcgaggtc
     5641 ctggaggaca ccatcgacgc ggtggtcggc gaccactgcc ccaacgactt cccggaggac
     5701 tgggacctcg acgagctgct cgcccaggtc aacctcatct acccgaccaa gttcacggcc
     5761 ggcgagctgc gcaccgcggc cacggccgcc at
//
LOCUS       DASWGG010000203         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_416594, whole genome shotgun sequence.
ACCESSION   DASWGG010000203 DASWGG010000000
VERSION     DASWGG010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416594"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1434
                     /locus_tag="VF954_03710"
     CDS             61..1434
                     /locus_tag="VF954_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEX9530220.1"
                     /translation="MEAHAASTARLGPRVVVAGTHSNVGKTTVATGLLAALRKRGVAV
                     AGAKVGPDFIDPGYHSLACGRPPRNLDAWMCGPGAVAPLAARAAAGAELLVVEGVMGL
                     FDGAADGTPASTADVARLLDAPVVLVVDAAAMSSSVAALVHGYLTHDPTVKLAGVILN
                     RVGSDVHEALLREALASQPVPVLGVLRRDDRMVWRDRHLGLVPVAEDPAAVAMTLERL
                     AAAVEAGVDVGAIAEVARAAPEMGTTWPPLPPPSGNPARFRPRVAMASGPAFTFAYTD
                     TVEALEAAGCEVVGFDPLTDEALPEATNGILIGGGFPEVYASQLGANRALLGELRAAG
                     VPVWAECGGLLVLCRSLDGQPMAGLVPADATMTERLTLGYRTATVARACALGPAGTTW
                     RGHEFHYSAVAPGGDALTLASRWGGRPEGWGGPDLLATYLHLHPGGDPAPVAAFAARC
                     FRQAGLA"
BASE COUNT          165 a          582 c          563 g          204 t
ORIGIN      
        1 gcaccggatt cctggcaccg tggcccggcc aacctaccag acgcctgtag cttcccaccg
       61 gtggaggccc acgcagccag caccgcccgc ctcggccccc gggtcgtcgt cgccgggacc
      121 cactccaacg tcggcaagac gaccgtggcc accggcctcc tcgccgcgct ccgcaagcgc
      181 ggcgtcgcgg tggcgggcgc caaggtgggc ccggacttca tagatccggg gtaccacagc
      241 ctggcgtgcg gccggccccc gcggaacctc gacgcctgga tgtgcggccc cggcgccgtc
      301 gcgccgctcg ccgcccgggc cgcggccggc gccgagctgc tggtggtcga aggggtcatg
      361 gggctcttcg acggggccgc ggacgggacg cccgcatcca cggcggacgt ggcccgcctg
      421 ctggatgccc cggtggtcct ggtggtcgac gcggcggcga tgtcatcgtc ggtggccgcg
      481 ttggttcacg gctacctcac ccacgacccg accgtgaagc tggccggagt catcctcaac
      541 cgggtgggct ccgacgtgca cgaggccctc ctgcgggagg cgctggcctc ccagcccgtt
      601 ccagtgctgg gcgtcctgcg acgggacgac cggatggtgt ggcgcgaccg ccacctcggg
      661 ctcgtccccg tcgccgagga ccccgcggcc gtggccatga ccctcgagcg gctggccgcg
      721 gcggtcgagg ccggcgtcga tgtgggcgcc atcgccgagg tggcccgcgc tgctccggag
      781 atgggcacca catggccgcc gctcccgccc ccgtccggaa acccggcccg tttccggccg
      841 cgggtggcga tggcctcggg gccggctttc accttcgcct acaccgacac cgtcgaggcg
      901 ctcgaggcgg ctggctgcga ggtggtcggc ttcgatccgc tcaccgacga ggccctgccc
      961 gaggccacga acgggatcct catcgggggc ggcttccccg aggtctacgc ctcccagctc
     1021 ggggccaacc gggccctgct cggcgagctg cgggccgccg gggtcccggt gtgggccgag
     1081 tgcggcgggc tgctggtgct gtgccgctcg ctcgacggcc agcccatggc gggcctggtg
     1141 cccgccgacg cgaccatgac ggagcgactg accctcggct accggaccgc cacggtggcg
     1201 cgggcgtgcg ccctggggcc ggccggcacg acgtggcggg gtcacgagtt ccactactcg
     1261 gcggtggcgc ccggcggtga cgccctcacg ctggccagcc ggtggggcgg ccggccggag
     1321 ggctggggcg gacccgatct gctggccacc tacctccacc tgcatcccgg tggcgacccg
     1381 gcgccggtgg cggccttcgc ggcccgctgc ttccgccaag ccgggctggc ctgaggctgc
     1441 cctacgtcgt accctacgtc ggtgcggaag acgagcgtct atctgccgga cccgctcaag
     1501 cgggcgctga gcgc
//
LOCUS       DASWGG010000204         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1172333, whole genome shotgun sequence.
ACCESSION   DASWGG010000204 DASWGG010000000
VERSION     DASWGG010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172333"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="VF954_03715"
     CDS             complement(<1..1523)
                     /locus_tag="VF954_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9530221.1"
                     /translation="MEEHQTDSGIDIRPVYTAEDLGDGLDGRLGRPGSAPYTRGVYEE
                     MYRKRRWTMRQYSGFGTADETNQRFHYLLKAGQTGLSCAFDLPTQMGYDSDHPRAEGE
                     VGKVGVAIDSLEDMERLLAGLPLAEVTTSMTINSTAAILLLFYELVAEKQGVPSTAIN
                     GTIQNDLLKEYIARGTYIYPPRQSMRIITDIFAYCRENIPRWNTISISGYHIREAGST
                     AVQEIAFTLSNAIAYVQAAIDAGLAVDEFAPRLSFFFNAHNNFFEEVAKFRAARRMWY
                     RIMTERFGAQDERSKLLRFHTQTGGSTLTAQQPENNVARVTLQALAAVFGGTQSLHTN
                     SFDEALGLPTERAAKIALRTQQIIGFESGVTETADPLGGSYFVESLTDAVEAEAWRYI
                     EKIDELGGSVPAIEAGFLQDEIERASYEWTKAVDDGRKVIVSVNRFAEDEALPAEVFP
                     IDPALERNQAQRLADLRARRDNAGVKAALEEVRAAARGTQNLLVPMREALKRLATLG"
BASE COUNT          230 a          534 c          582 g          274 t
ORIGIN      
        1 tccccgaggg tcgccaggcg cttgagggcc tcccgcatcg gcacgaggag attctgggtg
       61 ccgcgggccg cggcccgcac ctcttccagc gccgccttca cgccggcgtt gtcgcgccgg
      121 gcccgcaggt cggccagccg ctgggcctgg ttgcgctcaa gcgccgggtc gatggggaac
      181 acctcggctg gtagagcctc gtcctcggcg aagcggttga cgctgacgat gaccttccgg
      241 ccgtcgtcca cggccttcgt ccactcgtag gaggcccgct cgatctcgtc ctggaggaag
      301 ccggcctcga tggccggcac cgagccgccg agctcgtcga tcttctcgat gtagcgccag
      361 gcctccgcct ccacggcgtc ggtcagcgac tcgacgaagt acgacccgcc gagcgggtcg
      421 gcggtctccg tcacccccga ctcgaacccg atgatctgct gggtccgcag ggcgatcttg
      481 gccgcccgct cggtgggcag gccgagggcc tcgtcgaagc tgttggtgtg gagcgactgg
      541 gtgccgccga acacggcggc cagcgcctgg agggtcaccc gggcgacgtt gttctccggc
      601 tgctgggcgg tgagcgtgga gccgcccgtc tgggtgtgga accgcagcag cttcgagcgt
      661 tcgtcctggg cgccgaaccg ctcggtcatg atccggtacc acatgcgccg ggcggcccgg
      721 aacttggcca cctcttcgaa gaagttgttg tgggcgttga agaagaacga cagccggggg
      781 gcgaactcgt cgacggcgag gccggcgtcg atggccgcct gcacgtaggc gatggcgttg
      841 gagagggtga aggcaatctc ctggacggcc gtcgagccgg cctctcggat gtggtagccg
      901 gagatcgaga tcgtgttcca gcgggggatg ttctcccggc agtaggcgaa gatgtcggtg
      961 atgatccgca tcgactgccg gggcgggtag atgtacgtcc cccgggcgat gtactccttc
     1021 agcaggtcgt tctggatagt cccgttgatg gccgtcgacg gcacgccctg cttctcggcc
     1081 accagttcgt agaacagcag gaggatggcc gccgtcgagt tgatggtcat cgaggtggtg
     1141 acctcagcca gcggcaaccc ggccaggagg cgctccatgt cctccaggga gtcgatggcc
     1201 accccgacct tgccgacctc gccctcggcc cggggatggt cggagtcgta gcccatctgg
     1261 gtcggcaggt cgaaggcgca cgacagcccg gtctggccgg ccttcaggag gtagtggaac
     1321 cgctggttcg tctcgtcggc ggtgccgaac ccggagtact ggcgcatcgt ccaacgccgc
     1381 ttccggtaca tctcctcata gacgccccgg gtgtagggcg ccgacccggg ccggccgagc
     1441 cggccgtcga ggccgtcgcc gagatcctcg gcggtgtaga cgggccggat gtcgatcccc
     1501 gaatcggtct ggtgttcctc catggacgaa tcgtacgggg gcgcgtggca cgggccggaa
     1561 aaaaaatcgt tgtccccacc ggcgccgggc tgcctacgct gccccacggt ccgggcgctg
//
LOCUS       DASWGG010000205         9597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3101360, whole genome shotgun sequence.
ACCESSION   DASWGG010000205 DASWGG010000000
VERSION     DASWGG010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3101360"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1961)
                     /locus_tag="VF954_03720"
     CDS             complement(6..1961)
                     /locus_tag="VF954_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530222.1"
                     /translation="MSAPASGGRAAAVLAGGAGLAATAVAHWVFPFGSTDNDEGIYRL
                     QAEAMRHGHLFLPVPSAPRPFTPWLATVVDHHYVLKYTPLEPTLLAVSKLVSGSYLPA
                     LALVAASVVAATWALGREVFADGRKALAAAALVAACPLVVVQGGLLLAYLLTLSLVEV
                     FSWATLRAVRVGGSGRWAAPAGLALGLAASVRPYDAVLLGLPVVVWAGVRLHRRGSVA
                     GAARWFLAGGSGPAVGLGVYDRLATGHVFGLPFNLLEHRDNVGFGVRRLFPGDRPHHF
                     GVVEGLAGVGNHLGLLLVWVTGGPLLVLLAIAGWRRLARPRKGRGTPPESDLGNDSAT
                     DRGALWALAAAAGLLTIGYVAFWGVWNAADLWGGITMVGPFYLLPLVVPLCLLAAEGL
                     VALRSRPRLLVAAVAGLAAVTVAALIPAAVGDARASRQDGRLEALARRAPQLGGGAAL
                     VLVPGQGPFVMHPVGRLGNGWKVGGPLVVGAASGTSADLDALASVPGHAPFLLTLSRG
                     YSPRPAHPGGRLVRMVLTEAAAITLEVTPAGGRPAPAAALTVAAAGQTLSCGPADGDG
                     RWRVRLGGDGSVGCGATDDVLGAGALGPAPGGLRLAMSQELGLPPVSVLVATSVANGT
                     VRVLTAGDTVGTTGRPPPPVPLSVTAR"
     gene            complement(1958..2764)
                     /locus_tag="VF954_03725"
     CDS             complement(1958..2764)
                     /locus_tag="VF954_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530223.1"
                     /translation="MSSLNGDRRRIVGALWRGVATLVLAGLGGCLGQVACTGGRHVAV
                     LLYGDSLADEGQHWFEASFPSSVKVEVHVLGGTAICDFLPQLRGDVARARPAAVVFEF
                     AGNAVTRCTQPAPAVAATRQQLDERYRADAATATRLATAIGGEAWWMGAPVNRDPGAE
                     IGNGDIRAIYQGLARQYPRTHYVDAGAAVERDGQFTATLPCLPEEPCARVDGNENVVR
                     ADDGGHLCPSHQLFTDTCSVWSSGAYRFGRAMALPVLASLQLPPARVAMP"
     gene            2838..3803
                     /locus_tag="VF954_03730"
     CDS             2838..3803
                     /locus_tag="VF954_03730"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9530224.1"
                     /translation="MELSWQAGGEISAGLAALKVALTPVRDRRVAGARGVARELSLVC
                     GLYAVWRLAGTLSVMKVTHALARGRALWRLERFLHLPSELALQRGALGHPIWVELCNR
                     YYQLAHGPALMACLAWLFFRHRERYPRARNVIAAVTGAALAIQLIPVAPPRLLTGLGF
                     VDTGLLYHQSVYTALGQGMADQLSAMPSVHVAWAVMIAVFVVEASSSRWRFLVIAHPV
                     LTVWAVVVTANHFWLDGVVGAVLIVPALIAQDGVERWRARSGRQVLDGAGRATDEQPR
                     RDSTLLGASRRTGYESPDVPETAQPLSCSPMQLSGRGTPRPSWHR"
     gene            complement(3960..6275)
                     /locus_tag="VF954_03735"
     CDS             complement(3960..6275)
                     /locus_tag="VF954_03735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX9530225.1"
                     /translation="MAGVTTATTVTAEVEEQRHPDDVLPAGQGAGPGAGTWRHIPALD
                     GLRGVAVAAVLLYHGGIGWAKGGYLGVDAFFVLSGFLITSLLLDETARTGTVALARFW
                     SRRARRLLPAVLVLVAAVAGYAVFFAASGTLDGIRKDAIATLGYVANWRYIFAKASYF
                     ERTAPPSPLRHTWSLAIEEQFYVIWPLLVLAVARGSRSRLKIGVLAGTLAAGSVAAMV
                     LLFHPGQDPSRVYYGTDTRANVILIGALLAVIVASRRDGARKDAAHEDAANEDAAPSG
                     VPRSAVVPWLLGTAALGGAAGWLWAVVRVDGTASWLYRGGLPALALAIAAVIAHAVMV
                     PAGVTAKVLSLAPLRALGRISYGVYLWHWPLFLTLTRARTGLGGWSLFALRIAVTLAV
                     SMASYWAVELPVRRGLLPHWRSALALPAAVAGVCALVLATTASSMVPAAAILPAGTTG
                     GTTPPGTVPVPAGPSHRPPRPPGQPARVLVVGDSVAQTLADGLTPYASQYGLSIDNKG
                     TLGCGVVRGGPYRYFGQISQELPDCETWPDRWAQNVRREDPDVVMAILGRWEVMDRVH
                     DGRWSRIGDLDFDVYLETEIEHAVSVLSAGGAKVAFVTAPYYLRGERPDGGRWPEDDP
                     ARVDEWNRLLREVVSRHPAQASIVDLNYKTGPDGKYVNYIDGVKLRYDGVHFTPQGDH
                     WLAPWLLPQLAALGPPVVNGASPYTPLGTTPTIGSVTPTTRRRYVTSTSVYRSPSTTT
                     ATTRPVATTTHPPTTSPPTTAPYTSTTIRRR"
     gene            6453..7355
                     /locus_tag="VF954_03740"
     CDS             6453..7355
                     /locus_tag="VF954_03740"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HEX9530226.1"
                     /translation="MLTLIALLLAGCGVLTASNQTLLLGAAAIRPLATPTVLDAALAE
                     ASEVAHAPADLPAPKTPPPPPPPAPPARARAPGAPLKVMFIGDSIATTAADGLAPQAA
                     GFGINLIDDGIQGCGVVQGGPYNYFGSQHDPLPQCESWPSAWQADVTRDDPDLAVVVV
                     GRWELMDRFFEGRWTHIGDPAFDAYLANEVERAIAIVTTQGAKVALFTTPYYRRGLRP
                     DGGLFPEDDPARVDQMNGIFRAVAAKHPGTVSLVDFGGRLSPGGQLAMAIDGIPMRSD
                     GVHLTPQAGAWLAPWLLPQLTTIG"
     gene            complement(7413..7550)
                     /locus_tag="VF954_03745"
     CDS             complement(7413..7550)
                     /locus_tag="VF954_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530227.1"
                     /translation="MGRGALSRRRWKNDRVKRKKGRDLRAAVVRGEARKAEGAAAAAP
                     A"
     gene            complement(7583..8848)
                     /locus_tag="VF954_03750"
     CDS             complement(7583..8848)
                     /locus_tag="VF954_03750"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9530228.1"
                     /translation="MTAPNQLGTRRRSALRGLVAAAAISSTGDGLAAVAFPLVAIGLT
                     HDARLIAAVAAASRLPWLVCSLPAGALVDRCSARRVVAVTEGARTVLLLLTAWAVASH
                     AASVPLLCLVAFAVGTCETAFSAAVQSTVPALVAEQDLERANGRMYAAQMGGELFLGP
                     ALGGLAFAIGPSVPFVGDGLSFAVCGAVLLATLPRWRVDLIALERRSATLGADMLEGL
                     RWFLSHRLIRLLAVVVGSLAFFQAMVNGVAVVYAAHVLHLSKAGFGLFIAVGSVGNVL
                     GASVASRVKGRWGTGQLLAVAGVLAAVSYLVEGATSTTAVAVTVCAVESLVVLVGNVA
                     NLSLRQRIVPAQLRGRVSNLFRAVVYGAVPLGALFGGVVTEAAGVRAALVLAGAAQLA
                     VLGLTARALLREVAVYEPARPAVALAVAA"
BASE COUNT         1430 a         3568 c         3422 g         1177 t
ORIGIN      
        1 cgaggttatc gggcggtcac cgagagcggg acgggcgggg gcggccgccc ggtcgtgccg
       61 acggtgtcgc ccgctgtgag gacgcgcacg gtgccgttcg ccacggaggt cgccaccagc
      121 acgctcaccg ggggaagccc cagctcctgc gacatggcga gtcgcaaccc ccccggcgcc
      181 ggccccaggg caccggctcc cagcacgtcg tcggtcgccc cgcatcccac cgagccgtcc
      241 cctccgaggc ggaccctcca gcgcccgtcg ccgtcggccg ggccgcagga gagcgtctgg
      301 cccgcggcgg cgacggtgag ggcagccgcg ggggccgggc gccctccggc gggcgtgacc
      361 tcgagcgtga tcgctgcggc ttcggtcagc accatccgga ccaagcgccc gccaggatgg
      421 gcggggcgcg gcgagtaccc ccgagacagc gtgagcagga acggggcatg ccccggcacc
      481 gacgcgagag cgtccagatc ggcggaggta ccggacgcgg cgccgaccac gagggggccg
      541 cccaccttcc agccgttgcc caaccgcccg accggatgca tgacgaacgg gccctgcccc
      601 ggcacgagca ccaacgccgc acccccgccg agctgcggtg cccgccgggc cagcgcctcc
      661 agcctcccgt cctgccggct ggcccgggca tcccccaccg cagcggggat cagggcggcc
      721 accgtgacag cggccagacc ggcgaccgcc gcgacgagga ggcgggggcg cgacctcaga
      781 gccaccagcc cctccgcggc gagcaggcag agcgggacca ccagggggag aaggtagaag
      841 ggcccgacca tcgtgatgcc gccccacagg tcggccgcgt tccacacccc ccagaacgcc
      901 acgtagccga tggtcaacag accggccgcg gccgccagcg cccagagggc cccccggtcg
      961 gtcgcgctgt cgtttcccag atcgctctcc gggggtgtcc cgcgaccctt gcgcgggcgg
     1021 gccagccggc gccagccggc gatggcgaga agcaccagca acggcccccc ggtgacccag
     1081 acgagcagga gtcccaggtg gttgcccacg ccggccaggc cctccactac gccgaagtgg
     1141 tggggccggt cgccggggaa tagccggcgc acaccgaagc cgacattgtc tctgtgctcc
     1201 aacaggttga acggcagccc gaagacatgc ccggtcgcca gccggtcata gacgccgagc
     1261 ccgacagccg ggcccgaccc tccggcgagg aaccagcggg cggcacccgc cacgctcccc
     1321 cgccggtgaa gccgcacccc tgcccagacg acgaccggga ggccgagcag caccgcgtcg
     1381 tagggccgaa cggaggcagc cagccccaga gccaggccgg cgggcgctgc ccaccggcca
     1441 ctcccgccga cccgcaccgc ccgcagggtc gcccacgaga acacctccac caggctcaag
     1501 gtcagcaggt aggccaggag aaggccgccc tggaccacca cgagcgggca ggcggcgacg
     1561 agggcggcgg cggcgagggc cttgcggccg tcggcgaaga cctcgcgacc gagcgcccag
     1621 gtcgccgcga ccaccgacgc ggccaccagc gccaacgcgg gcaggtagga cccgctcacc
     1681 agcttgctca cggcgaggag ggtcggctcg agcggcgtgt atttcaacac gtagtgatgg
     1741 tcgacgacgg tcgccaacca cggggtgaac ggccggggcg cggacggcac cggaaggaag
     1801 aggtggccgt gcctcattgc ttcggcctgg agccggtaga tgccctcgtc gttgtctgtc
     1861 gagccgaaag ggaacaccca gtgggccacg gccgtcgcgg ccaggccggc accgcccgcc
     1921 aggactgccg cggcccggcc acccgatgcc ggggcgctca cggcatcgcc acgcgggccg
     1981 gggggagctg cagcgacgcc agcaccggca gcgccatcgc ccggccgaag cggtaggccc
     2041 ctgacgacca caccgagcac gtgtccgtga agagttggtg cgacgggcac aggtggccgc
     2101 cgtcatcggc gcgcaccacg ttctcgttgc cgtcgacgcg ggcgcacggc tcctccggca
     2161 ggcacgggag ggtcgccgtg aactgcccgt cgcgctcgac ggccgccccc gcgtccacgt
     2221 agtgggtgcg cgggtactgg cgtgcgaggc cttggtagat cgcccggatg tcgccgttgc
     2281 cgatctccgc gccgggatcc cggttcaccg gcgcacccat ccaccacgcc tccccgccga
     2341 tcgccgtcgc caaccgggtc gccgtggccg cgtcggctcg gtaccgctcg tcgagctgct
     2401 ggcgcgtggc cgccacagcg ggtgcgggct gggtgcaccg ggtgaccgcg ttcccggcga
     2461 actcgaacac gaccgcggcc ggccgcgctc gagccacgtc gccgcgcagc tgcggcagga
     2521 agtcgcagat ggccgtgccg ccgagcacgt gcacctcgac cttcaccgat gaggggaacg
     2581 acgcctcgaa ccagtgctgg ccctcgtcgg cgagcgagtc gccgtagagg agcacggcga
     2641 catggcgccc gccggtgcac gccacctgtc ccaggcagcc ccccaggccg gcgaggacca
     2701 gggtcgccac gccgcgccac agcgcgccga cgatccgccg ccgatcaccg ttgagggagg
     2761 acaccggcgg ccgacgctac cggtctggtc cgcccccctc gcgtagcctg tcgcggcgag
     2821 caagggtggc cgcattggtg gagttgtcct ggcaggccgg cggggagatc agcgccgggc
     2881 tcgccgcgct caaggtagcc ctaaccccgg tccgcgaccg ccgggtcgcc ggtgctcgcg
     2941 gggtggcccg cgagctgtcg ctggtctgcg ggctgtatgc cgtctggcgc ctggcaggga
     3001 ctctgtcggt gatgaaggtg acccacgccc tggcccgggg ccgggcgctg tggaggctgg
     3061 aacggttcct gcacctgccc agcgagcttg cgctgcaacg cggcgcgctg ggtcatccga
     3121 tctgggtaga gctctgcaac cggtactacc agctggcgca cgggccggcg ctgatggcct
     3181 gcctggcgtg gttgttcttc cgccatcgcg agcgctatcc ccgcgcccgc aacgtgatcg
     3241 cagccgtcac gggcgcagcc ctcgccatcc agctcatccc ggtggcgccg ccgcgcctgc
     3301 tgaccggatt gggtttcgtc gacaccgggc tgctctacca ccagagcgtg tacaccgcgc
     3361 tcggccaggg catggccgac cagctgtccg ccatgccctc cgtgcacgtg gcctgggcgg
     3421 tgatgatcgc cgtgttcgtc gtggaggcca gttcgagccg gtggcggttc ctggtcatcg
     3481 cccacccggt gctgaccgtg tgggccgtgg tggtgaccgc caaccacttc tggctcgacg
     3541 gcgtcgtcgg cgccgtcctg atcgtgcccg ccctcatagc ccaggacggc gtcgagcggt
     3601 ggcgggcccg gtcaggccgt caggtgctcg acggggccgg acgggcgaca gacgagcagc
     3661 cgcggcgtga ttcgaccctg ctcggtgcct cacggcgaac gggatacgaa tctccggacg
     3721 tacccgagac cgcccagccg ctcagctgca gcccaatgca gctctcaggg cgcggcacgc
     3781 cgcggccatc ctggcatcgc tgagcttggt caccggcctg cggaacgcct tgcggggcag
     3841 cgtccgcagg ttgtcgaagc tgaccacgga ctcgacgggc atgccgtcgt ccttgctgag
     3901 cggcagctcc gacacgaggt tgcgacgggg ttcgtgctcg ccggacactg ctcggctccc
     3961 tatctcctcc ggatcgtcgt gcttgtgtag ggcgctgtgg tggggggact ggtcgtgggc
     4021 gggtgggtcg tggtggcgac cggtctggtc gtggcggtgg tggtggatgg cgaacggtag
     4081 accgaggtac tggtcacgta ccggcgccgg gtcgtcggtg tgaccgagcc gatcgtcggg
     4141 gtggtcccga gaggcgtgta cggcgaggcg ccgttcacga cgggagggcc gagcgcggcc
     4201 agctggggca gcagccacgg cgccagccag tggtccccct gcggcgtgaa gtgcacgccg
     4261 tcgtagcgca gcttcacgcc gtcgatgtag ttgacgtact tgccgtcagg gccggtcttg
     4321 tagttgaggt cgacgattga cgcctgggcc gggtggcggg acaccacctc gcggagcagg
     4381 cggttccact cgtcgaccct ggcgggatcg tcctccggcc accggccgcc gtcggggcgc
     4441 tcaccccgga ggtagtaggg cgcggtcacg aacgccacct tggcgccgcc ggccgacagc
     4501 accgacaccg cgtgctcgat ctcggtttcg aggtagacgt cgaaatcgag gtcgccgatc
     4561 cggctccacc gtccgtcatg cacgcggtcc atgacctccc agcggccgag gatggccatc
     4621 acgacgtcgg gatcctcccg gcgcacgttc tgggcccacc ggtcgggcca ggtctcgcag
     4681 tcgggcagct cctggctgat ctggccgaag taccggtacg gcccgccccg gaccacccca
     4741 cacccgagcg tccccttgtt gtcgatggac agcccgtact gggaggcata cggcgtgagc
     4801 ccgtcggcga gggtctgggc caccgagtca ccgacgacga ggacgcgggc cggctgcccg
     4861 ggcgggcgcg gcggccgatg gctcggcccg gcaggcaccg ggaccgtgcc cggcggggtg
     4921 gtgccgccgg tggtgccggc cggcaggatg gcagccgcgg ggaccatcga cgacgccgtg
     4981 gtggccagca ccagggcgca cacaccggcg acggcggcgg gaagcgccag ggccgagcgc
     5041 cagtggggca gcagcccgcg cctgaccgga agctcgactg cccagtacga cgccatcgac
     5101 accgccaggg tcacggcgat ccgcagggcg aagaggctcc agcccccgag gccggtccgg
     5161 gcccgggtca gggtcaggaa gagcggccag tgccacaggt agacgccgta ggagatccgg
     5221 cccagggcgc gcagcggggc cagggagagg accttggccg tgaccccggc cggcaccatc
     5281 acggcatggg cgatgaccgc ggcgatggcg agggcgaggg cgggcaggcc gccccggtac
     5341 agccagctcg cggtgccgtc cacccgcacc acggcccaca accaccccgc cgccccgcct
     5401 agggcggccg tccccaggag ccacggcacc acggccgacc gggggacgcc cgacggggcg
     5461 gcgtcctcgt tggcggcgtc ctcgtgggcg gcgtccttcc gggccccgtc gcgccgcgac
     5521 gcgacgatca cggcgagcag ggcgccgatg aggatgacgt tggcccgggt gtcggtgccg
     5581 tagtacaccc ggctcgggtc ctggccggga tggaacagca ggaccatcgc cgccaccgag
     5641 ccggcggcca gggtcccggc cagcacgccg atcttcagcc tgctccggct gccccgggcg
     5701 acggccagca cgagaagcgg ccagatcacg tagaactgct cctcgatggc cagcgaccac
     5761 gtgtggcgca ggggcgacgg cggggcggtg cgctcgaagt agctggcctt ggcgaagatg
     5821 taccgccagt tggcgacgta gcccaaggtg gcgatggcgt ccttgcggat cccgtcgagc
     5881 gtccccgatg ccgcgaagaa cacggcatag ccggccaccg ccgccaccag caccagcacc
     5941 gcgggcagca gccggcgggc ccgcctcgac cagaaccggg cgagggcgac cgtcccggtc
     6001 cgggcagtct cgtcgaggag cagcgacgtg atcaggaacc ctgagaggac gaagaaggcg
     6061 tccacgccca gatagccgcc cttggcccag ccgatgcccc cgtggtacag gagcacggcc
     6121 gccacggcga cgccccgcag cccgtcgagg gccgggatat ggcgccacgt ccccgcgccc
     6181 ggaccggccc cctgcccggc aggcaggacg tcgtcgggat ggcgctgctc ttccacttcc
     6241 gccgtcacgg tcgttgccgt cgtcaccccg gccattggcc tctctcgtag tcgccttgcg
     6301 cccctggcgc ggtcagaaaa aggttacccg cgccaccgtg accgtcgatg catgggccat
     6361 gcggaccacc gaccgccgtg ggtgagcatc tatcattgac gttcgtgcac gagtggacac
     6421 tgggggcaca tcgctcccga cacgcccctc gagtgctgac gctcatcgcg ttgctcctcg
     6481 cgggctgcgg ggtattgacc gcgtccaacc agaccctgct gctcggcgcg gcggccatcc
     6541 ggcccctcgc cacccctacc gtcctggacg cggcgctggc cgaggccagc gaggtggccc
     6601 acgcccccgc cgacctgccg gcgccgaaga cgccccctcc cccgcccccg ccggctccgc
     6661 ccgcacgggc ccgggcaccc ggggcaccac tcaaggtgat gttcatcggg gactcgatcg
     6721 ccaccacggc ggccgacggg ctggccccgc aagccgcggg gttcggcatc aacctgatcg
     6781 acgacgggat ccagggctgc ggggtcgtcc agggcggccc gtacaactac ttcggctccc
     6841 agcacgaccc gctgccgcag tgcgagtcct ggccgtcagc ctggcaggcc gacgtcaccc
     6901 gcgacgaccc cgacctggcc gtcgtggtcg tcgggcggtg ggagctgatg gaccggttct
     6961 tcgagggccg ctggacccac atcggcgacc cggccttcga cgcctacctg gccaacgagg
     7021 tcgagcgggc catcgccatc gtcaccaccc aaggggccaa ggtcgccctc ttcacgacgc
     7081 cctactaccg gcggggcctg cgccccgacg ggggactgtt ccccgaggac gacccggccc
     7141 gggtggacca gatgaacggc atcttccggg cggtggcggc caagcacccc ggcaccgtca
     7201 gcctggtcga cttcggcggc cggctctccc ccggcggcca gctggccatg gccatcgacg
     7261 gcatccccat gcgcagcgac ggggtccacc tcacgccgca ggcgggcgcc tggctggccc
     7321 cctggctgct gccccagctg acgaccatcg gctgacccct acacgagcct gccgatgtgg
     7381 gattagccgg ccggttcagc ccgggcgaac gcctacgccg gcgcggccgc cgcggcgccc
     7441 tcggccttgc gggcctcgcc ccgaacgacc gcggcccgta ggtcacggcc cttcttcctc
     7501 ttgacccggt cgttcttcca gcggcggcgg ctgagcgctc ctcgtcccat ggggccatgg
     7561 tagaggcgca gccggccgaa ccctaagccg cgaccgccaa ggccacggcg ggccgggcgg
     7621 gctcgtacac cgccacctca cggagcaggg cccgggccgt gagcccgagc acggcgagct
     7681 gggccgcccc cgccagcacc agtgccgccc gcacaccggc ggcctcggtc accactcccc
     7741 cgaagagggc cccgagcggc acggccccgt acaccacggc ccggaagagg ttgctcaccc
     7801 gcccccggag ctgcgccggc acgatccgct gccgcagcga aaggttggcc acgttgccca
     7861 ccaggaccac gagcgactcc acggcgcaca ccgtcaccgc cacggcggtc gtcgaggttg
     7921 cgccctccac caggtagctc acggccgcca ggaccccggc gacggcgagc agctggcccg
     7981 tgccccaccg ccccttgacg cggctggcga ccgacgcgcc caggacgttg ccgaccgagc
     8041 cgacggcgat gaacagcccg aaaccggctt tggacaggtg caggacatgg gcggcgtaga
     8101 cgacggccac gccgttcacc atggcttgga aaaaggccag gctgccgacc accaccgcca
     8161 gcagccggat gaggcggtgc gacaggaacc accgcaggcc ctcgagcatg tcggcgccga
     8221 gcgtggccga cctgcgctcc agggcgatca ggtcgacgcg ccagcggggc aaagtggcca
     8281 gaaggacggc gccgcagacc gcgaacgaca ggccatctcc cacgaacggg acgctgggcc
     8341 cgatggcgaa ggccagccct ccgagggcgg gcccgaggaa gagctctccg cccatctggg
     8401 cggcgtacat ccggccgttg gcccgctcca ggtcttgttc tgctaccagg gccggcacgg
     8461 tcgactggac agcagcagag aacgccgtct cgcacgtgcc cacggcgaag gccaccaggc
     8521 agagcagcgg caccgacgcc gcgtggctgg cgaccgccca cgccgtgagg agcagcagga
     8581 ccgtgcgggc gccctcggtc acggccacga cccggcgcgc cgagcaccgg tcgaccaggg
     8641 cccctgcggg cagggagcac accagccagg gcagccggct cgccgcggcc acggcggcga
     8701 tgagccgggc gtcgtgggtc aggccgatgg cgaccagcgg gaaggcgacg gcggccaggc
     8761 cgtcgcccgt gctcgagatc gcagccgcgg ccaccaggcc gcgcagggcg gaacggcgcc
     8821 tggtaccgag ttggttggga gcagtcatcg cgtcctagaa aaaaacactg cttgtggccg
     8881 gatcagcact aaaggtaggt gccgacccgg ccgacaagcc tagaggttgg ttcaccgatc
     8941 gctgtacgct tcgcaaacgt tcgatcggag ttcccttaca tggtggtcgt ctgagacctg
     9001 ctcgacaggt ctcagcccca cgatccgctc cggcgggaca ttggctctca cctccccccc
     9061 gagttcggag ccgtgcccgt tggggcggct gagataagga gaagagcgcg agcgcggctc
     9121 cggcgggtcg ggcccgcgat ttcggacgag attcacggcg tgttacgtcc caccttgaac
     9181 gagatcgaac tggcagccag gccgtgtctg gacccgcgac aacgagagtg cagctcgcat
     9241 tcccgtgccc ccgcagcagc aaggtcattg tggcagccct tggggcccgc gtctacaccc
     9301 gcggggctga tttgatgaaa actggccgtc ccaactaggg ctattcctgg ccaaacccgg
     9361 catcttggct atggccatat gacgccgacg gactagtcct ccatcgaggg gtaaagccgg
     9421 ccaaccccgg tgccgacagg aaagggtgca aagggcatcg ggccgcgcca agaagcaggc
     9481 acgtgcggcc gtggcctcct tcgcggcccc gaccccggtc ctcgtcgtgg tggcgctcct
     9541 cgtcctggcg gcggcggcgc tctgggccgg ccccgccctg cactccttcc ggcccgg
//
LOCUS       DASWGG010000206         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_417935, whole genome shotgun sequence.
ACCESSION   DASWGG010000206 DASWGG010000000
VERSION     DASWGG010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417935"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1561)
                     /locus_tag="VF954_03755"
     CDS             complement(<1..1561)
                     /locus_tag="VF954_03755"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9530229.1"
                     /translation="MTSTVTGPVPALARREPPRATAARRPAWPRRRRPGDWLRLALAS
                     TLALVSAWAADRAAPTIVEINVFRLVNQLPAPVGAPLLGIMQAGALLAVPAFALLALL
                     GGRRRLAAIVLWGGLTAWALAKLGQWLVDEDPPILRLSRVVQHGVAHTGTSFPSTHVA
                     VAAGLAAVAGPYLARPGRRLLWLVTALVAVARLYVGLHLPVDVVGGLALGWGAGAALN
                     LIWGVPAPGPSTAHVADVLGDLGLQARAIEQLPPERDRYRFRCRMGSGPDLLVKAADQ
                     EAAGDDWLWRLWRLVAFRELPDPVVGGTPAERAEHEAYVHLLAQRAGVRTPPLIATRT
                     LDGPAALVVREWVDADAVGARELTDAELLDAWSQLSSAHAGGVVHGGLTASHVLVDRD
                     HRAWLVEWGTGKASTDPAHRAADVAELAVSLSLASPPELVVQAAARALPSDALADAVQ
                     HLQPLALAPFVGRQLADRPGLLDELRELLAATAGVPAPVTLSPARAVGRNLLPLVAVG
                     VAVSVLLPRLSK"
BASE COUNT          244 a          650 c          718 g          154 t
ORIGIN      
        1 ccttggacag ccgcggcagc aggacggaga cggcgacacc gacggccacc aagggcagca
       61 ggttgcggcc caccgcccgg gcgggcgaga gggtcacggg cgcggggacg ccggcggtgg
      121 cggccaggag ctcgcggagc tcgtcgagca ggcccgggcg gtcagccagc tgccggccga
      181 cgaagggcgc cagggccagc ggttgcagat gctgcaccgc gtccgccagc gcgtcggagg
      241 gcagggcccg ggcggcggcc tgcacgacga gctcgggcgg gctggcgagg ctcagcgaga
      301 cggccagctc ggccacgtcc gcagcccggt gggcgggatc ggtggacgcc ttcccggtcc
      361 cccactccac gagccacgcc cggtggtcgc ggtcgaccag cacgtggctg gccgtcagcc
      421 ccccgtggac caccccaccg gcatgggccg acgacagctg gctccaggcg tccagcagct
      481 cggcgtcggt gagctctctc gcgccgaccg cgtcggcatc gacccactcc cgcaccacga
      541 gggcggcggg accgtcgagg gtgcgggtgg cgatcagcgg gggcgtgcgg acaccggccc
      601 gctgggccag gaggtggacg taggcctcgt gctcggcacg ttcggccggg gtgccaccca
      661 ccacagggtc cggcagctcg cggaaggcca ccagccgcca cagccgccac agccagtcgt
      721 cgccggcggc ctcctggtcc gcggccttca cgagcaggtc cgggcccgag cccatgcggc
      781 accggaaccg gtagcgatcg cgctccggag ggagctgctc gatggcccgg gcctgcaggc
      841 cgaggtcgcc cagcacgtcg gcgacgtgcg cggtggacgg gcccggggcg ggcacgcccc
      901 agatgaggtt cagggcggcc ccggcacccc agccaagggc caggccgccg acgacgtcga
      961 ccgggaggtg caggcccacg tagagccggg cgacggccac gagggcggtc acgagccaca
     1021 gcagccggcg gccggggcgg gccaggtagg gcccggcgac cgcggccagg ccggcggcca
     1081 cggccacgtg ggtggagggg aagctggtgc cggtgtgggc cacgccgtgc tggacgaccc
     1141 gggacagccg caggatcggc gggtcctcgt cgaccagcca ctggcccagc ttggccagcg
     1201 cccaggcggt caggccgccc cacagcacga tggcggccag gcgccgtcgg ccgcccagca
     1261 gggcgaggag cgcgaaggcc gggacggcca gcagggcccc ggcctgcatg atcccgagga
     1321 ggggggcgcc cacgggagcc ggcagctggt tgacgagacg gaagacgttt atctcgacga
     1381 tcgtcggcgc agcccggtcg gccgcccacg ccgagaccag cgccagcgtc gaggcgagag
     1441 ccaggcgcag ccagtccccg gggcgacgcc ggcggggcca cgccggccgg cgggcggcgg
     1501 tcgcccgggg cggctctcgc cgggcgagag caggcaccgg cccggtgacg gtcgaggtca
     1561 tcgtgcccag cgtgccaccg aagaggcccc gggtgggggc ggcctagccg ccgcggctgg
     1621 gggtgagcgg ccggcggtcg gcaccacccg gggcggcgtc gagacccagg ccggcgtcga
     1681 gacccagggc ggcgtcgagc tgcccctcga tctcggccca cgtcagcagc tccccacccg
     1741 aacggcgggc cggctcctct gagcgg
//
LOCUS       DASWGG010000207         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1176262, whole genome shotgun sequence.
ACCESSION   DASWGG010000207 DASWGG010000000
VERSION     DASWGG010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176262"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..524
                     /locus_tag="VF954_03760"
     CDS             195..524
                     /locus_tag="VF954_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530230.1"
                     /translation="MPGAASGSRSADHHSLSALPSVRARVAAFVAIVAAGGFGGLIGY
                     GFAGVQCRAGCTNGKGVGLLVGGVIASAGVAVVSVLVLRAMGEWRTIRESAGPGPGAK
                     GPPGGRV"
     gene            complement(526..1269)
                     /locus_tag="VF954_03765"
     CDS             complement(526..1269)
                     /locus_tag="VF954_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX9530231.1"
                     /translation="MAELMADVPGSALAVFAHPDDPEVACGGTLARWSAAGAEVRVVI
                     CGSGDKGSLDPATDRSALVVTRAAEVASAAEHLGLAGHHLLGHPDGELDAGPELRGEL
                     VGLIRRFRPEVVICPDPTAVFFGQHYVNHFDHRNVGWATLDAVAPCAGMPLYFPEAGP
                     PHQVAWLLLSGTLDPDVWVDITSSIERKATAVACHASQLAGGPDGPDGPAGPAGEQAV
                     IAMVRQRAEEAGRAAGVRYAEGYRRIRVG"
     gene            complement(1302..>1556)
                     /locus_tag="VF954_03770"
     CDS             complement(1302..>1556)
                     /locus_tag="VF954_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530232.1"
                     /translation="DHQRARRLAEAVAERWPGAGVDPDRVLTNIVTFPHSDPTALIVH
                     LAAERVLAGTIAPGRMRLVTHHDVDDEGVERAAKALAGAP"
BASE COUNT          191 a          589 c          558 g          218 t
ORIGIN      
        1 acttcaatga ccccgccggg cgtcgaccag tcgagtgagc cgtcccgccg acggttctgg
       61 acgaggagga ctcgcccctc cgcttcgatg agaccaccgg ctaccaccca ctgccgcata
      121 cctcattgtg gcctcatcgc gacacgctct cggcgcgtcc gtgtgggggc gcctccgccc
      181 gtagcatctc tgccgtgccc ggtgccgcca gcggaagccg ctcggccgac caccactcgc
      241 tgtccgccct cccgtcggtg cgcgcccggg tggcggcgtt cgtggccatc gtcgccgccg
      301 gcggcttcgg tgggctcatc ggctacgggt ttgccggcgt ccagtgccgg gcgggatgca
      361 ccaacggcaa aggagtcggt ttgctcgtcg gtggcgtcat cgcctcggcc ggggtcgcgg
      421 tcgtgtcggt cctggtgctc cgggcgatgg gcgaatggcg cacgatccgg gagtccgccg
      481 gccctggccc cggggccaaa ggcccgcccg gagggcgtgt ctagctcagc ccacccggat
      541 ccgccggtac ccctctgcgt agcgcacacc ggccgcccgc ccggcttcct cggcccgctg
      601 gcgcaccatg gcgatcacgg cctgctcgcc cgccggtccg gccggtccat ccggtccatc
      661 cggtccgccc gcgagctggc tggcgtggca cgccacggcg gtcgccttgc gctcgatgga
      721 ggacgtgatg tccacccaga cgtcgggatc gagcgtgccg gagagcagca gccacgccac
      781 ctggtggggg ggcccggcct cggggaagta cagcggcatg ccggcacagg gcgccaccgc
      841 atcgagcgtc gcccacccga cgttgcggtg gtcgaagtgg ttgacgtagt gctggccgaa
      901 gaagacggcc gtggggtccg ggcagatcac cacctcgggg cggaaccggc ggatgaggcc
      961 cacgagctca ccccggagct cgggaccggc gtcgagctcc ccgtcgggat ggccgaggag
     1021 gtggtgcccg gccaggccga gatgctcggc ggcgctggcc acctcggcag ccctggtgac
     1081 caccagcgcc gaccgatcgg tggccggatc gagcgagccc ttgtcgccgc tgccgcagat
     1141 cacgaccctg acctctgccc cggcggccga ccatcgagcc agcgtgccgc cgcacgccac
     1201 ctcgggatcg tccgggtggg cgaacacggc gagcgcgctc cccggcacgt ccgccatcag
     1261 ctcggccatc aaccgagcgt agccacgccg ccgcgccggc cctacggggc gcccgccagc
     1321 gccttggccg cccgctcgac gccttcgtcg tcgacgtcgt ggtgggtgac gaggcgcatc
     1381 cgcccgggcg cgatcgtgcc ggccaggacc cgctccgccg ccaggtggac gatcagcgcc
     1441 gtcgggtcgg agtgggggaa ggtcacgatg ttggtcaaca ccctgtccgg gtcgactccc
     1501 gctcccggcc agcgttcggc gaccgcttcc gcgaggcggc gggcccgctg gtggtc
//
LOCUS       DASWGG010000208         3234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1491546, whole genome shotgun sequence.
ACCESSION   DASWGG010000208 DASWGG010000000
VERSION     DASWGG010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491546"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VF954_03775"
     CDS             complement(<1..1139)
                     /locus_tag="VF954_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9530233.1"
                     /translation="MRRSTLRPLALVGPIVLLAGACGGGGKGGGGGGTAGGPGTTAAA
                     MASQCPKGAVAQSAQKPVQITMWHSMTQANLQALTKLTDRFNASHTDIHVNLVNQTSY
                     ADTLTKYKAVLGGGARPDMVQIQDVDQQLVIDSHSVLPVEACIQAEKIDTSGFVPRVL
                     SYYTVRGVQYAMPFATSNPVLYYNKQSFQKAGLDPEKPPATLDEVRADAQKIVASGAD
                     KYGIALKNEGSLIEEWSAMAGALYANNGNGRTARATGVELGGPTGQGIYSWLSGMARD
                     KLAESTSVTGFDNLLAIGNNVAAMTIETSAALGTIFQVLSSGQYRNVTLGVAPLPGPV
                     AGGPTIQAGSALYVVSGTSPARQEAAFMFAEWLSEPAQTAEWSAAT"
     gene            complement(1153..2019)
                     /locus_tag="VF954_03780"
     CDS             complement(1153..2019)
                     /locus_tag="VF954_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9530234.1"
                     /translation="MSGGRAVSGDRAAARRRRAAIRALRYAVLVAVAAVVLFPVYITV
                     VNSLLTPDVIGHRPPTLFPVHPHGSAYVTAWHQGHMGTYLRNSFVVALAITAGQVATS
                     ILAGYAFALVRFPLRTPLFVVFLSTLMVPFEATIITNRHTIVSLGWVNRYPALVVPFL
                     ATGLGTFLLRQAFLGVPRELQDAARLDGYGHCRFMLRVAVPLARPSVAALGVFSFLGA
                     WNQYLWPLLVTDTDRLRTVQIGLRQLEGESIQQISVTFAGTVLAAVPIFVLLVIFQKQ
                     LVRGLTAGAVKG"
     gene            complement(2027..3019)
                     /locus_tag="VF954_03785"
     CDS             complement(2027..3019)
                     /locus_tag="VF954_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9530235.1"
                     /translation="MADPAEPTAAEPTAAEPTAAEPIATPQPEGPPGRRTTVAQRRRR
                     EAALGYALVAPALVVFGVFVFYPLVRTIQLGFYRAAPFPGLPNKAVGLSQYRRVLGSS
                     DFRSSLKVTVLFGLYTVPTGIVLGTLLALLAHQRLRAIGLFRTIFSSTVATSVAVASV
                     IFFTLLNPQVGLFSYWLGKVGGNGVLQDPRWALPAVAVATVWQNLGFTFVLLSAALQS
                     IPDELLEAARVDGAGAWPRFWQVTLPLLSPTLMFAAVIGGIAAFQAFGQIDLLTQGGP
                     LNHTRVLTYYLYQEAFLRNNDGVAAVLAVALFVISLVLTLLQLRLLERRVFYAR"
     gene            complement(3003..>3234)
                     /locus_tag="VF954_03790"
     CDS             complement(3003..>3234)
                     /locus_tag="VF954_03790"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX9530236.1"
                     /translation="VIAPGGSLPATVAVVESLGHERHVACRLADGALWIALVASSAAC
                     PAIGEPIGLSARPGGLHIFDPADGKRLDGRPG"
BASE COUNT          511 a         1058 c         1236 g          429 t
ORIGIN      
        1 gtggcggcgg accactccgc ggtctgggcc ggctccgaca gccactcggc gaacatgaac
       61 gccgcctcct ggcgggccgg ggaggtcccc gacacgacgt acagagccga gccggcctgg
      121 atggtgggcc ctccggcgac cgggccgggg aggggggcca cgccgagggt gacgttgcgg
      181 tactggccgg agctgagcac ctggaagatc gtgcccaggg cggcggacgt ctcgatggtc
      241 atggcggcca cgttgttgcc gatggcgagc aggttgtcga acccggtgac cgacgtgctc
      301 tcggccagct tgtcgcgggc catgccggac agccacgagt agatgccctg cccggtaggt
      361 ccgccgagct cgaccccggt tgcccgggcg gtgcgcccgt tgccgttgtt ggcgtagagc
      421 gcgccggcca tggcggacca ctcttcgatg agcgagcctt cgttcttgag ggcgatgccg
      481 tacttgtcgg ctcccgacgc cacgatcttc tgggcgtcgg cccgcacctc gtcgagggtg
      541 gcggggggct tctccggatc gaggccggcc ttctggaacg actgcttgtt gtagtacagg
      601 accgggttgg aggtggcgaa gggcatggcg tactgcaccc ctcgaaccgt gtagtaggac
      661 aggaccctcg gcacgaagcc cgaggtgtcg atcttctccg cctggatgca cgcctcgacc
      721 ggcaggacgc tgtggctgtc gatcaccagc tgctggtcga cgtcctggat ctgcaccatg
      781 tcgggccggg ccccgccgcc cagcacggcc ttgtacttgg tcagggtgtc ggcgtagctg
      841 gtctggttga ccaggttcac atggatgtcg gtatgggacg cgttgaaccg gtcggtcagc
      901 ttggtcaggg cctggaggtt ggcctgggtc atcgagtgcc acatggtgat ctgcacgggc
      961 ttctgggccg actgggccac cgcgccctta gggcactggg acgccatggc ggccgcggtc
     1021 gtcccggggc cgcccgccgt gccgccaccc ccgcccccct ttcccccgcc cccgcacgcc
     1081 ccggccagga gcacgatcgg cccgacgagg gccagcgggc ggagcgtgga tcggcgcatg
     1141 aacgtgccct ccctagccct tcacggcacc ggccgtgagg cctcggacca gctgcttctg
     1201 gaagatgacc agcaagacga atatcggtac cgcggccagg acggtacccg cgaacgtcac
     1261 gcttatctgc tggatgctct cgccttccag ctggcgcagg ccgatctgaa cggtgcggag
     1321 cctgtccgtg tcggtgacga ggaggggcca gaggtactgg ttccacgcgc cgaggaagga
     1381 gaagacgccg agcgcggcga cgctcgggcg ggcgagcggc acggccaccc gcagcatgaa
     1441 gcggcagtgg ccgtaaccgt cgaggcgggc cgcgtcctgc agctcgcggg gcacgccgag
     1501 gaaggcttgg cgcaggagga aggtcccgag accggtggcg aggaacggga ccaccagggc
     1561 ggggtagcgg ttgacccacc cgaggctcac gatggtgtgg cggttggtga tgatcgtggc
     1621 ctcgaagggc accatgaggg tggacaggaa gaccacgaag agcggggtgc gcagcgggaa
     1681 gcgcaccagg gcgaaggcgt agccggccag gatcgacgtg gccacctggc cggcggtgat
     1741 ggcgagggcc accacgaagc tgttgcggag gtaggtcccc atgtggccct ggtgccacgc
     1801 cgtcacatag gcgctgccgt gcgggtggac cgggaagagg gtcgggggcc ggtgaccgat
     1861 gacgtcaggc gtcagcagcg agttgacgac cgtgatgtag accgggaaca gcacgacggc
     1921 ggccacggcg acgagcacgg cgtaccggag ggcccggatg gcggcgcgcc gccgcctggc
     1981 cgcggcgcgg tcaccggaca cggcgcggcc accggacatg gcgcggtcac cgggcataga
     2041 agacccgccg ttcgagcagt cgcagctgga ggagcgtgag caccaggctg atcacgaaca
     2101 gggccacggc gagcacggcc gccacgccgt cgttgttgcg caggaaggcc tcctggtaca
     2161 ggtagtaggt gaggacgcgg gtgtggttga ggggaccgcc ctgggtcagc aggtcgatct
     2221 ggccgaaggc ctggaaggcg gcgatcccac cgatcacggc cgcgaacatc aacgtcggcg
     2281 acagcagcgg cagcgtcacc tgccagaacc ggggccacgc gcccgcccca tccacccggg
     2341 ccgcctcgag cagctcgtcg gggatcgact ggagcgccgc gctgagcagg acgaaggtga
     2401 atccgaggtt ctgccagacg gtggcaaccg ccaccgcggg cagggcccac cggggatcct
     2461 ggaggacccc gttgccgccg accttgccga gccagtagct gaacagcccg acctgcgggt
     2521 tcagcagggt gaagaagatg accgacgcga ccgcgaccga cgtggccacc gtcgaggaga
     2581 agatcgtccg gaacagcccg atggcgcgca gccgctggtg ggccaggagg gcgagcaggg
     2641 tgccgagcac gatgccggtc ggcacggtgt agaggccgaa cagcacggtc accttcaggc
     2701 tggatcgaaa gtccgacgac cccagcacgc ggcggtactg gctgagcccg accgccttgt
     2761 tgggcagtcc ggggaaaggg gcggcccggt agaagccgag ctggatggtc cgcaccagcg
     2821 ggtaaaagac gaagaccccg aacaccacca gggccggggc gacgagggcg tagcccaggg
     2881 cggcctcccg ccggcgccgc tgggccaccg tggtccggcg cccgggcggc ccttccggct
     2941 ggggcgtcgc gatgggctcg gccgcggtgg gctcggccgc ggtcggctcg gccgcggttg
     3001 gctcagccgg gtcggccatc gagccgcttg ccgtccgcgg ggtcgaagat gtgaaggcct
     3061 ccgggccggg cgctcagccc gatgggctcg ccgatggccg ggcaggccgc gctcgacgcc
     3121 accagcgcga tccacagcgc gccgtcggcc aggcggcagg ccacgtggcg ctcgtggccg
     3181 agggactcga ccaccgcgac cgtcgccggt agcgagccgc cgggcgcgat cacc
//
LOCUS       DASWGG010000209         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3106507, whole genome shotgun sequence.
ACCESSION   DASWGG010000209 DASWGG010000000
VERSION     DASWGG010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3106507"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..420
                     /locus_tag="VF954_03795"
     CDS             100..420
                     /locus_tag="VF954_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX9530237.1"
                     /translation="MKENHRRSVVNRLRSARGHLDAVLRMVEEDAYCPDVMKQLSAVQ
                     GQLEGANRLVLRNHLETCVAAAMVAGRTGEIVDELMEAMRFDRITTGPDRAGTARSRT
                     KVPS"
     gene            417..>1956
                     /locus_tag="VF954_03800"
     CDS             417..>1956
                     /locus_tag="VF954_03800"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX9530238.1"
                     /translation="MSRSGTAAAERRPTSDAEAVALAGLDLDIGGMTCASCAARIEKV
                     LARQPGVSRAGVNYATARANVAYDPARTGLHALTAEVERIGYQATPVASASERPPDAE
                     RAEERAWLRRVLVAWPLGITVFVLLLVDMGAPWARWTAAVLTLPIQFWAGWPFLRSAA
                     VRARLREANMDTLIAIGTLAAFGFSAWRLFGGGDLYFDTAALIIAFLLLGRFFEARAR
                     GRASSAIRALLELGAKEARLVIDGDGDGDERMVPVEQVGVGDVVRVRPGEKVPVDGEV
                     LEGRSAVDESMLTGESVPVDKGPGDRVTGATVNRQGVLTVRATAVGANTALAQIVRLV
                     EEAQGGKAPVQRLADRVAGVFVPAVMAVAVATALGWALAGDPTQGLVSAVAVLIIACP
                     CALGLATPTAIMVGTGRGASLGVLIKGGEILERSKRIDTVVFDKTGTLTEGQMSLTDL
                     RPAAGVDHDELLALAAAAESGSEHPIGRAVVDGARARGLAVPDVTDFEALAGHGVRAA
                     VAGRT"
BASE COUNT          263 a          724 c          686 g          283 t
ORIGIN      
        1 cgggacgctg tacgtgcgca cggtcatctc ggcccgccct ccatcgcatt cgtcgtcact
       61 gtggaaatat accccacccc ccctggggta gagtaaggca tgaaggaaaa ccaccgtcgg
      121 tcagtcgtca accggttgcg cagtgcccgc ggtcacctcg acgcggtcct gcgcatggtc
      181 gaggaggacg cctactgccc agacgtgatg aaacagctgt ccgcagtgca gggccagctc
      241 gagggggcca accggctggt gctgcgcaac cacctggaga cctgcgtcgc cgcggccatg
      301 gtcgccggcc ggaccggtga gattgtggac gaactgatgg aagccatgcg cttcgaccga
      361 atcaccaccg gccccgaccg agccgggacc gccagatcac gcacgaaggt gccgtcgtga
      421 gccgcagcgg caccgcggcc gccgagcgcc gaccgacgag cgacgccgag gccgtcgctc
      481 tggccgggtt ggacttggat atcggtggga tgacctgcgc gtcgtgcgcg gcgcgcatcg
      541 agaaggtcct cgcccgccag cccggcgtgt cccgcgccgg ggtcaactac gccaccgccc
      601 gggccaacgt ggcctacgac cccgcgcgca ctggcctcca cgcgctcacc gccgaggtcg
      661 agcgcatcgg ctaccaagcc acgcccgtcg catcggcctc cgagcgtccg cccgacgccg
      721 aacgtgcgga ggagcgggcc tggctgcgac gggtgctcgt cgcctggccg ctcgggatca
      781 ccgtgttcgt actgctgctc gtcgacatgg gcgccccctg ggcccggtgg acggccgcgg
      841 tcctcaccct gccgatccag ttctgggccg gctggccctt cctccgcagc gccgcggtca
      901 gggcccggct gcgtgaggcc aacatggaca ccctcatcgc catcggcacc ttggcggcgt
      961 tcggtttctc ggcctggcgc ctgttcggcg gcggcgacct gtacttcgac accgccgcgc
     1021 tgatcatcgc cttcctgctg ttgggccgct tcttcgaggc ccgagcccgc ggccgcgcct
     1081 cgtcggccat ccgggcccta ctcgagctcg gcgccaagga ggcccggctc gtcatcgacg
     1141 gcgacggcga cggcgacgag cggatggtcc cggtcgagca ggtcggcgtc ggcgacgtcg
     1201 tgcgggtccg gcccggtgag aaggtccccg tcgacggcga ggtgctcgag gggcgctcgg
     1261 cggtcgacga gtccatgctc accggcgagt ccgtccccgt cgacaaggga cccggtgacc
     1321 gggtgaccgg cgccaccgtc aaccgccagg gcgtcctcac cgtccgggcc actgccgtcg
     1381 gtgccaacac cgccctggcc cagatcgtgc gcctggtcga ggaagcccaa ggcggcaagg
     1441 cccccgtcca gcgcctcgcc gaccgagtcg ccggcgtgtt cgtccccgcc gtcatggccg
     1501 tcgcggtggc gaccgcgctc gggtgggcgt tggccggcga tccgacgcag ggcctggtgt
     1561 cggcggtagc ggtgctgatc attgcctgcc cgtgcgccct cgggctggcc acgccgaccg
     1621 ccatcatggt cggcaccgga cgcggcgcca gcctcggcgt gctcatcaag ggcggggaga
     1681 ttctggagcg ctccaagcgc atcgacactg tcgtgttcga caagaccggc accctgaccg
     1741 aaggacagat gagtctgacc gacctgcgcc cagccgccgg cgtcgatcac gacgagctcc
     1801 tggcgctggc cgcggcggcc gagtcgggct ccgagcaccc gatcggtcgg gccgtggtcg
     1861 acggcgctcg cgctcggggc ctcgccgttc cggatgtcac cgacttcgaa gctctcgccg
     1921 gccacggggt gcgggccgcg gttgcgggcc gcaccg
//
LOCUS       DASWGG010000210         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3107137, whole genome shotgun sequence.
ACCESSION   DASWGG010000210 DASWGG010000000
VERSION     DASWGG010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3107137"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VF954_03805"
     CDS             complement(<1..966)
                     /locus_tag="VF954_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9530239.1"
                     /translation="MYPGTYATSTPDKPAYIMAATGEAVTFKQLDDRSNQVAQLLWER
                     GLRPGDSVAVFMENNPAFFEITWAAQRSGLYYTAVSSRLTAPEVAYIVNDCGAKAFFT
                     SAARADVAVDVLAEIPDAEVRVMVGDPAAGPPSAAEAAGFEDYAEEVGRQPPEPLEHE
                     IEGQDMLYSSGTTGRPKGVRVPLSNAPAGSGGFGVTLILQGLYGMTADDVYLSPAPLY
                     HAAPLRFSMSAQRLGATVVVMERFDPVEFLEVVQRYRITYTQVVPTMFVRLLKLPEQV
                     RTSFDLSSLVSVVHAAAPCPPAVKRQMIDWWGPIVREYYGGSETGP"
     gene            1086..2375
                     /gene="tyrS"
                     /locus_tag="VF954_03810"
     CDS             1086..2375
                     /gene="tyrS"
                     /locus_tag="VF954_03810"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX9530240.1"
                     /translation="MSALFAELEWRGLVHQVTSPEVADRLDHGALTAYIGFDPTADSL
                     HIGHLLQLCNLRRLQEHGHRPIALAGGGTGMIGDPGGKVEERSLLTEEALTANLAGIR
                     DQLGRFLDFSPSAGAAQARLVDNGDWLWKEGLLAFLRDVGKHFTVNQMITRESVRARL
                     EQREHGISFTEFSYMLLQAYDYLQLFDRYGCTLQMGASDQWGNITLGVELIRKVRGAE
                     AFGLTTPLVLKPDGTKFGKTESGAVWLDGRRTSPYELFQFFVRSADAVVGRYLRYYTW
                     LPLERVEELEKATVEHPERRQAQLALARAVTTLVHGTGEAAAAERASQVLFGGGELAG
                     LDERTLLQVFSEAPSTERSRESLDGPGVSLVEALQESGLVASRSAARTTIGQGGVYVN
                     NRKETDADRRLTAGDLVGGRYVVLRRGKREHHLLRFG"
     gene            complement(2455..2658)
                     /locus_tag="VF954_03815"
     CDS             complement(2455..2658)
                     /locus_tag="VF954_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530241.1"
                     /translation="MVVTPSSTIPAPPPTSAPTNGPSGHISAVFPILSAGGLGLVVAI
                     FGLQAWLTRPRRPRRRGRRRWTL"
     gene            2648..>2912
                     /locus_tag="VF954_03820"
     CDS             2648..>2912
                     /locus_tag="VF954_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530242.1"
                     /translation="MTTTVVVVEEDVVVELARRVVGGPAGRVVDGAARGAVVVGAAGA
                     VVGWDVCVVGVVVGRTWARAPGPDSPAAANATTAATRRPARQAL"
BASE COUNT          420 a          983 c         1110 g          399 t
ORIGIN      
        1 cggcccggtc tccgagccgc cgtagtactc ccgcacgatc gggccccacc agtcgatcat
       61 ctgccgcttc accgccggcg ggcagggtgc cgccgcgtgc accaccgaaa ccagcgagga
      121 caggtcgaag gaggtacgca cctgctcggg cagcttgagc agccggacga acatggtggg
      181 cacgacctgg gtgtaggtga tccggtacct ctgcacgacc tccaggaact cgaccgggtc
      241 gaatcgctcc atcaccacga cggtcgcccc gaggcgctgg gcgctcatgg agaaacgcag
      301 gggggcggcg tggtacagcg gggcgggcga gaggtacacg tcgtcggcgg tcatgccgta
      361 caggccctgc aggatcaggg tcaccccgaa gccgccgctg cccgccgggg cattgctcag
      421 cggcacgcgg acgcccttgg gccgcccggt ggtgccggac gaatagagca tgtcctggcc
      481 ctcgatctcg tgctcgaggg gctccggcgg ctggcggccg acctcctcgg cgtagtcctc
      541 gaagccggcc gcctcggccg cgctgggggg gccggccgcg gggtcgccga ccatgacccg
      601 cacctcggcg tcggggatct cggcgagcac gtccacggcc acgtcggcgc gggcggccga
      661 ggtgaagaag gccttggccc cgcagtcgtt gacgatgtag gccacctcgg gagcggtcag
      721 ccgcgagctc acggccgtgt agtacaggcc ggagcgctgg gccgcccagg tgatctcgaa
      781 gaaggcgggg ttgttctcca tgaagacggc gacgctgtcg cccggccgca gccctcgctc
      841 ccacaggagc tgggccacct ggttggaccg gtcgtccagc tgcttgaagg tcaccgcctc
      901 gcccgtcgcg gccattatgt aggccggctt gtcgggcgtg gaggtggcgt aggtgcccgg
      961 gtacatgcct gtcgacgcta ccgaccgggg gtgaggacgg acaaagccgc tcggtcctct
     1021 ggccagctcg gtgctctggc cggctcggtg ctctggccgg ctcggtcctc tggcaggctg
     1081 gggccgtgag cgcgctgttc gccgagctgg agtggcgggg cctggtccac caggtgacca
     1141 gccccgaggt ggccgaccgc ctcgaccacg gcgctctcac ggcctacatc ggcttcgacc
     1201 cgacggccga cagcctccac atcgggcacc tgctccagct ctgcaacctg cggcgtctcc
     1261 aggagcacgg gcaccggccg atcgccctcg ccggtggggg cacgggaatg atcggcgacc
     1321 ccggcggcaa ggtcgaggag cgcagcctgc tgaccgagga ggccctgacc gccaacctcg
     1381 ctggcatccg cgaccagctg ggccgcttcc tcgacttctc cccctccgcc ggcgcggcgc
     1441 aggcccgcct cgtcgacaac ggcgactggc tctggaagga ggggctcctg gcgttcctcc
     1501 gggacgtggg caagcacttc accgtcaacc agatgatcac cagggagtcc gtccgggccc
     1561 gcctggagca gcgggagcac ggcatctcat tcaccgagtt cagctacatg ctcctccagg
     1621 cctacgacta cctccagctg ttcgaccgtt acggttgcac cctccagatg ggggccagcg
     1681 accagtgggg caacatcacc ctgggcgtcg agctgatccg caaggtgcgg ggggcggagg
     1741 ccttcgggct gacgacgccg ctggtgctga agcccgacgg gaccaagttc ggcaagaccg
     1801 agtccggcgc ggtttggctc gacgggcggc ggacgagccc gtacgagctg ttccagttct
     1861 tcgtgcggtc ggcggatgcc gtcgtggggc ggtacctgcg gtactacacg tggctgccgc
     1921 tcgaacgggt cgaggagctg gagaaggcca ccgtggagca tccggagcgc cgccaggccc
     1981 agctggcgct ggcccgggcc gtcaccaccc tcgtgcacgg gaccggcgag gcggccgcgg
     2041 ccgagcgggc gtcgcaggtc ctgttcggcg ggggagagct ggccgggctc gacgagcgga
     2101 cgctgctcca ggtcttctcc gaggcgccct cgaccgagcg gtcccgggaa tccctcgacg
     2161 ggcccggcgt gagcctggtc gaggccctcc aggagtcggg cctggtggcc tcccgctcgg
     2221 cagcccggac caccatcggc cagggagggg tgtacgtcaa caaccgcaag gagaccgacg
     2281 ccgaccgccg gctcacggcc ggggacctgg tgggagggcg ctacgtggtg ctgcggcggg
     2341 ggaagcggga gcaccatctg ctgcgcttcg gctagctgct agaggcggtg tggcaccaaa
     2401 accattcttc aagcaaaaaa accgctggga cggcattaaa gcatggaaga atgctcaaag
     2461 cgtccagcgg cggcggcccc ggcggcgcgg gcggcgcggg cgcgtcagcc aggcctgcag
     2521 cccgaagatc gccaccacga ggcccaggcc gccggcggag aggatgggga aaacggcgct
     2581 gatgtggccg gacgggccgt tggtcggagc ggacgtgggt ggcggggcgg ggatggtcga
     2641 gctcggtgtg acgaccacgg tggtcgtggt cgaggaggat gtggtcgtcg agctggctcg
     2701 ccgggtcgtc ggggggcccg ccggccgggt ggtggacggc gcggcgcgag gggccgtggt
     2761 ggtcggcgcc gccggggcgg tggtcggctg ggacgtgtgc gtggtcggcg tggtggtggg
     2821 aaggacctgg gcccgggccc cgggcccgga cagcccggcg gcggcgaacg ccacgacggc
     2881 ggccaccagg cgacccgccc gccaggccct ca
//
LOCUS       DASWGG010000211         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3107224, whole genome shotgun sequence.
ACCESSION   DASWGG010000211 DASWGG010000000
VERSION     DASWGG010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3107224"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VF954_03825"
     CDS             complement(<1..337)
                     /locus_tag="VF954_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9530243.1"
                     /translation="MSATVPVVSTRVQILSEALHCFSAHGYEGTSLNDIAAAVGIRRP
                     SLLHHFPSKEALYREVFELYLADWFVRVEKASLDPKDGLAKVDYVLTAAFQFFMDNPE
                     FVRLVRREAL"
     gene            457..1200
                     /locus_tag="VF954_03830"
     CDS             457..1200
                     /locus_tag="VF954_03830"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX9530244.1"
                     /translation="MRPLLLALLIGFALCQLGTLLTTVYLHRALSHRSLTLRPGLRWA
                     CRILLWVTTGIRPRQWVAVHRKHHAFTDVEGDPHSPVLKGFAAVQFGNVGLYRKAARD
                     PETAARFARDLPADRWDRALFDHALLGLGLGIALLVLAFGWEVGLLAAGAHTVLYLLL
                     NAAVNAVGHTWGRQPFANTSHNSQWLAWLTVGEGLHNNHHAAPTSAKLALARREIDPA
                     WPLIALARRLGWLTVRHDEVRLKREKLAA"
     gene            1241..>1682
                     /locus_tag="VF954_03835"
     CDS             1241..>1682
                     /locus_tag="VF954_03835"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX9530245.1"
                     /translation="MSFGSALSQHPVTAHAVGEVAGQVLEATGPGPDLAVLFVTSGHA
                     GALEDAAAAVTSVLAPAALIGCAAVSVVGTGQEVEEQAGVSLWAGAVGPVAPLELDAR
                     RSPSGTAIDGLSEQALSGGRAVLLLADPFSFPAEVAFEALARQCP"
BASE COUNT          214 a          627 c          588 g          253 t
ORIGIN      
        1 cgagggcctc gcgccgcacc aggcgcacga actcggggtt gtccatgaag aactggaagg
       61 cggcggtcag cacgtagtcg accttggcca ggccgtcctt cgggtccagg ctggccttct
      121 cgacccgcac gaaccagtcg gccaggtaca gctcgaagac ctcgcggtac agggcctcct
      181 tcgaagggaa gtggtgcagc aggctgggcc ggcggatgcc gaccgcggcg gcgatgtcgt
      241 tgagagacgt gccctcgtag ccgtgtgcgg agaagcaatg cagggcctcg gaaaggatct
      301 ggacacgcgt cgagaccacg gggacggttg cggacatcgc gcttcagggt acccgggcgc
      361 cgtgcccgga cacggtgacg agggacttcg ggtctgccgg gagatgtccg gctccctacc
      421 taccggtcgg taggcagccc ctggtagcgt gggccgatgc gccccctctt gctggccctg
      481 ctcatcgggt tcgcactgtg ccagctgggc accttgctga ccaccgtcta ccttcaccgg
      541 gcgctgtcgc accgatcgct caccctgcgg ccggggctgc gctgggcgtg ccgcatcctg
      601 ctctgggtca ccaccgggat ccggccccgc cagtgggtgg ccgtgcaccg caagcaccac
      661 gccttcaccg acgtcgaggg cgacccacac tccccggtcc tcaagggctt cgccgcagtg
      721 cagttcggca acgtcgggct ctaccgcaag gcagccaggg acccggagac ggccgcccgc
      781 ttcgcccggg acctcccggc cgaccggtgg gaccgggctc tgttcgacca tgcgctgctc
      841 ggcctgggcc tcggcatcgc cctgctggtg ctggccttcg gatgggaggt cggcctgctc
      901 gccgccggag cccacaccgt cctttacctg cttctcaacg cggccgtgaa cgcggtcggc
      961 cacacctggg ggcgccagcc cttcgccaac acctcccaca acagccagtg gctggcctgg
     1021 ctaacggtcg gcgagggcct gcacaacaac caccacgccg caccgacctc ggcgaagctg
     1081 gccctggcgc ggcgggagat cgacccggcc tggccgctga tcgcgctggc ccggcgtctc
     1141 ggatggctga cggtccgcca cgacgaggtt cgtctgaagc gggagaagct ggcggcctga
     1201 gccgccgcgg ggccgcgccg ccggcgcata tcttcgcgcc atgtccttcg gctccgccct
     1261 gtcccagcat ccggtcacgg ctcacgccgt gggcgaggtg gccggccagg tcctcgaggc
     1321 caccggcccc ggcccggacc tggccgtgct cttcgtcacc tccggtcacg ccggggcgct
     1381 agaggacgcc gccgcggccg tgacctccgt gttggcgccg gccgccctga tcggctgcgc
     1441 ggcggtgtcg gtggtcggca ccggccagga ggtcgaagag caggccggtg tgtcgctgtg
     1501 ggccggcgcc gtcggcccgg tcgcgcccct ggagctcgac gcccgccggt ccccgagcgg
     1561 gaccgccatc gacggcctgt ccgagcaagc cctgtccggc ggccgtgccg tcctgctgct
     1621 cgccgaccct ttctcgttcc cggccgaggt ggccttcgag gccctcgccc ggcagtgccc
     1681 cg
//
LOCUS       DASWGG010000212         3484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2354113, whole genome shotgun sequence.
ACCESSION   DASWGG010000212 DASWGG010000000
VERSION     DASWGG010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354113"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VF954_03840"
     CDS             <1..438
                     /locus_tag="VF954_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530246.1"
                     /translation="PGPAGVRLGIAGVLAAAVDTRVLAVRLRPARRQVNEDWLDRYRG
                     WVYAAGFGAQLGAGLVTIVTSATVYLTVVAAFLTGSARQGVLVGSAFGLSRAVPVLFT
                     GRVRTPLQLRAWHARLASWRGPVEWLTVAGTMGAAAALLGSRW"
     gene            435..908
                     /locus_tag="VF954_03845"
     CDS             435..908
                     /locus_tag="VF954_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530247.1"
                     /translation="MTRLAGRGLALELPVGWEGHIYRRAEDGSGSTQPVMHVASFPLP
                     AARGDFGGGAVERMGPDDVFVALLEFHPSSAGAALFSSPPPAALGLGDFAPSSQQTFV
                     EGNSAAQRFFSVGDRAFCLYAVIGSHARRTALVPLVNRVLSGLAVTPLDRLGGSR"
     gene            905..1441
                     /locus_tag="VF954_03850"
     CDS             905..1441
                     /locus_tag="VF954_03850"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX9530248.1"
                     /translation="MTAAVLTGPLVAAAILLAMAGGIKLFNPSPLARSLRLAGLPAGL
                     ARPSPVRVAAAAEMLLGIATVLGGGRALGGGLAAATLAAYLAFAAFLVAARRRGGPLT
                     SCGCFGRTDTPVTRAHITLDAAAAIVSGGGFTARAPGLPAALASHPGAAPALLAAVAA
                     LVAVAYVAFAVAPAAARP"
     gene            1438..2844
                     /locus_tag="VF954_03855"
     CDS             1438..2844
                     /locus_tag="VF954_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX9530249.1"
                     /translation="MTAPASQRLVDHLAGVLERRTSRRGFLARTAIVGSALAVAPAEY
                     ILRPVSAYAAACGCSGQGCACGSACCDGYTEFCCTINQGANTCPAGSFVGGWWKADGS
                     VYCSGARYYIDCMAMCTGCGCSGGHFCSPACDGLTCRCANGNCNLRKVGCISFRYGQC
                     HQEIACSGRIACRVVTCTPPWMVDPTCTTTVLVDDNTANHDAPCLQEPTVDFGSYGVA
                     VRAQGGYYAVAADGGVFAEGGAPFFGSMGGQVLRAPVLGIAATATGGGYWLVAADGGV
                     FAFGDAPYLGSMGGSYLNGPIIAMAATPTGKGYWLLGRDGGLFCFGDAAFHGSTGAMS
                     LNAPIVGMAPTPTGRGYWLVGSDGGIFCFGDAAFHGSTGDLKLNRPIAGLFPTPSGGG
                     YYLLARDGGVDAFGDAPFFGSYPGLPGDEQTLPPLQSGAFFSLLVKMTGAHVSGYTIY
                     AVSAPAPPPAVASWAFGS"
     gene            2897..3367
                     /locus_tag="VF954_03860"
     CDS             2897..3367
                     /locus_tag="VF954_03860"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9530250.1"
                     /translation="MELTGTAVLAAPARRVLPWVADLSTYPMWLSMVTSADPAPRLVG
                     DDGPAWAVGIGARLGPLRRSKRLRMVRTVHAGSRVRFERREVDGREHGAWLLDAALAE
                     EGARATLLTMRLEYRGAAWVGLLEAVLRQEVRRAGPRLDRLVTGERREGETTQE"
BASE COUNT          365 a         1317 c         1259 g          543 t
ORIGIN      
        1 ccgggccccg ccggcgtgcg cctggggatc gccggcgtgc tggcggccgc cgtcgacacc
       61 cgggtgctcg ccgtcaggtt gagaccggca cggcgccagg tgaacgagga ctggctcgac
      121 cggtaccggg gttgggtcta cgccgccggc tttggcgccc agctcggcgc cgggctggtc
      181 accatcgtca cctccgccac cgtctacctg acggtggtcg ccgccttcct cacgggctcg
      241 gcgcgacagg gtgtgctggt gggatctgcg ttcgggctca gccgggctgt gcccgtgctc
      301 ttcaccggcc gggttcggac tccgcttcag ctgcgggcgt ggcacgcccg gctggccagc
      361 tggcgcggcc ccgtcgagtg gctcacggtc gccgggacga tgggcgcggc agccgccctt
      421 ctggggagcc ggtggtgacc cgcctggccg ggcgaggcct cgccctcgag ctgcccgtcg
      481 gctgggaggg ccatatctac cgccgggcgg aagacggctc cggctcgacc cagcccgtga
      541 tgcacgtcgc cagcttccct ctgccggccg cccggggcga tttcggcgga ggggcggtgg
      601 agcgcatggg cccagacgac gtcttcgtcg ccctgctgga gttccaccct tcgagcgcgg
      661 gcgccgccct gttctcctcg ccgcccccgg ccgccctcgg gctcggggac ttcgccccct
      721 cgtcgcagca gaccttcgtg gagggcaaca gcgccgccca acgcttcttc tcggtcggcg
      781 atcgggcctt ctgcctgtac gccgtgatcg gttcccatgc ccgtcgcacc gcgctggtcc
      841 ccctcgtgaa ccgggttctg tccggtctgg cagtcacccc gctcgaccgg ctcggtgggt
      901 cgagatgacg gcggcggtcc tgaccgggcc tctcgtggcg gcggccatcc tgctggccat
      961 ggccggcggc atcaagctgt tcaacccgtc gcccctggct cgcagcctcc ggctggccgg
     1021 gctccccgcg gggttggcgc gcccgtcccc ggtgcgggtg gcggcggccg ccgagatgct
     1081 cctgggcatc gccacggtcc tcggcggagg gcgggcgctc ggcggcggcc tcgccgccgc
     1141 caccctggcc gcctacctgg cattcgccgc ctttctggtc gcggcccggc ggcgaggcgg
     1201 accgctcacg tcgtgcggct gcttcggcag gacggacacg ccggtgaccc gggcccacat
     1261 aaccctcgac gccgccgccg ccatcgtctc gggcgggggc ttcaccgccc gcgcccccgg
     1321 gctgcccgcc gcgctcgcct cccatccggg cgccgccccc gccctcctcg ccgcggtcgc
     1381 cgccctggtg gcagtcgcct acgtggcgtt cgcggtggcg ccagcggcgg cgcggccgtg
     1441 accgcgccgg ccagccagcg cctggtcgac cacctggccg gcgtgctgga gcggcgtacg
     1501 agccggcggg gtttcctggc ccggaccgcg atcgtcggct ccgctctcgc cgtggccccg
     1561 gccgagtaca tcctgcgccc cgtctcggcc tacgccgcgg cgtgcgggtg ctccgggcag
     1621 ggctgcgcct gcggctcggc gtgctgcgac ggctacaccg agttctgctg caccatcaac
     1681 cagggcgcca acacgtgccc cgcgggctcg ttcgtcggcg gctggtggaa ggccgacggc
     1741 tcggtgtact gcagcggcgc ccgttactac atcgactgca tggccatgtg caccggctgc
     1801 ggctgctcgg gcggtcattt ctgctcgccg gcgtgcgacg gcctcacctg caggtgtgcg
     1861 aacggcaact gcaacctccg caaggtgggg tgcatctcct tccgttacgg ccagtgccat
     1921 caggagatcg cctgctcggg ccggatcgcc tgccgggtcg tgacctgcac gccgccgtgg
     1981 atggtggacc cgacctgcac gacgaccgtg ctcgtcgacg acaacacggc caaccatgac
     2041 gccccgtgcc tccaggagcc cacggtcgac ttcggttcct acggagtagc cgtccgggcc
     2101 cagggtggct actacgccgt cgcggccgac ggcggcgtgt tcgccgaggg cggagcgccc
     2161 ttcttcgggt ccatgggcgg ccaggtactg cgcgcgccgg tcctcggcat cgccgcgacg
     2221 gccaccggcg ggggttactg gctggtcgcg gccgacggcg gcgtcttcgc cttcggcgac
     2281 gccccctacc tcgggtcgat gggagggtcg tacctcaacg gaccgatcat cgccatggcc
     2341 gccaccccga ccgggaaggg ctactggctg ctcggccgcg acggcggcct cttctgcttc
     2401 ggcgacgccg ccttccacgg ctcgaccggt gccatgtcgc tcaacgcccc gatcgtcggg
     2461 atggcgccca cgccgacggg taggggctac tggttggtgg gcagcgacgg cggcatcttc
     2521 tgcttcggcg acgccgcctt ccacggctcg accggcgatc tgaagctcaa ccggcccatc
     2581 gccgggctgt tccccacgcc gtcgggcggc ggctactacc tcctggcccg ggacggcgga
     2641 gtggacgcct ttggcgacgc gcccttcttc ggctcgtacc ccgggctgcc cggcgacgag
     2701 cagacgctgc cgccgctcca gtccggtgcg ttcttctcgc tcctggtcaa gatgaccggc
     2761 gcccacgtca gcggctacac catctacgcc gtgtcggcgc ccgccccacc tcccgcggtg
     2821 gcgtcgtggg ccttcggctc gtgaccgcat ccgagccgcc gccgcctcct ccaggaagcg
     2881 gataggccag ataggcatgg agctgaccgg cacggccgtg ctcgccgcgc ccgcccgacg
     2941 cgtcctgccc tgggtggccg atctgtccac ctacccgatg tggctctcga tggtcacctc
     3001 ggccgatccc gcgccgcgcc tggtgggcga cgacgggccg gcatgggcgg tcggcatcgg
     3061 cgcccggctc ggcccgctgc ggcgcagcaa gcggctgcgg atggtgcgga ccgtccatgc
     3121 cggcagccgc gtgcggttcg agaggcgcga ggtggacggg cgcgagcacg gggcctggtt
     3181 gctggatgcc gccctggcgg aggagggcgc ccgcgccacc ctgttgacga tgcggttgga
     3241 gtaccgcggc gcggcctggg tggggctgct ggaggccgtc ctgcgccagg aggtccgccg
     3301 ggccgggccc cgcctggacc gcctggtcac gggcgagcgc cgggagggcg agactaccca
     3361 ggagtaacac cgcaattcct gggtatggat gtgaccagtg acacacgcgg ccccagtggg
     3421 aggggtagtt ctatgagacg gtccgtgctt gcccggcgct acgcgcccct gatcgccctt
     3481 ggag
//
LOCUS       DASWGG010000213         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2354848, whole genome shotgun sequence.
ACCESSION   DASWGG010000213 DASWGG010000000
VERSION     DASWGG010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354848"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /locus_tag="VF954_03865"
     CDS             <1..1453
                     /locus_tag="VF954_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9530251.1"
                     /translation="FVADMQVPDPDVFYEIEGNVVTTSTYEGLVRYKPDSNQIEPALA
                     ASYTTAPDGMTYTFKLRSGVAFHDGSGTMTSGDVKASFERRTALGSVSAPGYMLADVT
                     GYDTPDPLTFVVHLKNPVAPFLDYLAAPYGPKVEDAAGLAKNAGTDMAQAFLKTHDLG
                     TGPFTMTDFVPGDHYVLSRFAGYWGGQAAVATIHISILPDISTQQLELKGGQLEMILH
                     GLSKDDIASYESDKKFQVVRFPALFKTWLMVNENKGVFKDQALRTALRSAVNKQQIVD
                     QVYGQDATVSTQYYPAGELPPTMAVDKPVYDPTILAKAVAGLANKKVDLAYSSDDARN
                     QRDAEIVQTELQTAGFAATVRGIPIAQVFDLPNHPDQAPDVLLSTVNPDAAHPDTWAR
                     IFSNTKGTLNWLLCSDPAADAQMDVGQHATTVSAVQAAYASAGDELVKSGCFISIADV
                     KDVVVAAAGYSSWAHQLPTVFTVRFGDLKLSGS"
     gene            1509..>2496
                     /locus_tag="VF954_03870"
     CDS             1509..>2496
                     /locus_tag="VF954_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9530252.1"
                     /translation="MTAISAEARIVGSRAAPAAAPVVALDRLHVTFERGGRRIRALRG
                     VSLRIGPGQILGLVGESGSGKSVLGLSLLGLLPTEPAPDVEGQAVVCGLDMVDASPDE
                     RRRLRREHLGAVFQDPMTSLNPTMRVGRQVAEAAGSESEAVRLLDSVGVPEARRRLRA
                     FPHELSGGLRQRVMIAMAVAGQPSLVIADEPTTALDVTVQAQILELVRHLCDQTGTSF
                     LLVTHDLGVAAQVADHIAVLYAGRLAEIGASDDVLRRPAHPYSRGLLQSRLTLTARRE
                     GPLPTLPGEPPDPRAQPPGCPFAPRCDLREDRCEEELPDLEPSGRHPGWAACL"
BASE COUNT          383 a          955 c          814 g          344 t
ORIGIN      
        1 cttcgtggcc gacatgcagg tgccggaccc ggacgtcttc tacgagatcg agggcaatgt
       61 cgtcaccacc tcgacgtacg agggcctggt ccgttacaag cccgactcca accagatcga
      121 gcccgccctg gccgccagct acacgacggc gcccgacggc atgacctaca ccttcaagct
      181 gcgttcgggg gtggccttcc acgacgggtc ggggaccatg acctcgggcg acgtgaaggc
      241 cagcttcgag cggcgcacgg ccctcggctc ggtctcggcg ccgggctaca tgctcgccga
      301 cgtgaccggc tacgacacgc ccgaccccct gaccttcgtg gtccacctga agaacccggt
      361 ggcgcccttc ctcgactacc tcgcggcccc gtacgggccg aaggtcgagg acgccgccgg
      421 cctggccaag aacgccggga cggacatggc ccaggcgttc ctgaagaccc acgacctcgg
      481 aacaggaccg ttcacgatga cggacttcgt acccggcgac cattacgtgc tcagccgctt
      541 cgccggctac tggggcggcc aggccgcggt ggccacgatc cacatctcga tcctcccgga
      601 catctccacc cagcagctcg agctcaaggg cggccagctc gagatgatcc tccacggcct
      661 gtccaaggac gacatcgcca gctacgagag cgacaagaag ttccaggtgg tgcgcttccc
      721 cgccctcttc aagacctggc tcatggtcaa cgagaacaag ggcgtgttca aggaccaggc
      781 cctgcgcacg gccctgcgct cggccgtcaa caagcagcag atcgtcgacc aggtctacgg
      841 ccaggacgca acggtctcca cccagtacta ccccgctggg gagctgccac cgacgatggc
      901 tgtcgacaag cccgtctacg accccaccat cctggccaag gccgtcgccg ggctggccaa
      961 caagaaggtc gacctggcct actccagcga cgacgcccgc aaccagcgcg acgccgagat
     1021 cgtccagacc gagctccaga ccgcgggctt cgcggccacc gtgcgcggca tcccgatcgc
     1081 ccaggtcttc gacctgccca accatcccga ccaggcgccc gatgtcctgc tgtcaaccgt
     1141 caacccggac gcggcccacc ccgacacctg ggcccgcatc ttcagcaaca ccaaggggac
     1201 gctcaactgg ctgctgtgct cggaccccgc ggccgacgcc cagatggacg tgggccagca
     1261 cgccaccacc gtctcggccg tgcaggcggc ctatgccagc gccggcgacg agctggtcaa
     1321 gtcgggctgc ttcatcagca tcgcggacgt gaaggacgtg gtcgtggccg ccgccggcta
     1381 ctcgagctgg gcccaccagc tcccgacggt gttcaccgtc cgcttcggcg acctcaagct
     1441 cagcgggtca tgagaacgca acacggcggt gaccgtgccg acacgaagcc cgcctaccgt
     1501 ccgttgacgt gacggccatc tcggctgagg ctcgcatcgt cgggtctcgc gcggcgccag
     1561 ccgccgcccc cgtcgtggcg ttggaccgtc tccacgtcac cttcgagcgc ggcggccggc
     1621 gcatccgggc cctgcgcggc gtgtccctgc ggatcgggcc ggggcagatc ctcggcctgg
     1681 tcggcgagtc cggctcgggc aagagcgtgc tcgggctctc cctgctgggg ctgctgccca
     1741 cggaaccggc gccggacgtg gagggccagg ccgtcgtgtg cggactggac atggtggacg
     1801 cgtccccgga cgagaggcga cgtttgcggc gagagcatct gggggccgtc ttccaggacc
     1861 cgatgacctc cttgaacccc accatgcggg tcggccgcca ggtggccgag gcggccggga
     1921 gcgagtcgga ggcggtgcgc ctcctcgact cggtcggggt ccccgaagcg aggcggcgcc
     1981 tgcgggcctt cccccacgag ctgtcgggcg gcctacgcca gcgggtgatg atcgccatgg
     2041 ccgtggccgg gcagccgtcg ctcgtcatcg ccgacgagcc gaccaccgcc ctcgacgtga
     2101 ccgtgcaggc gcagatcctc gagctggtgc ggcacctgtg cgaccagacc gggacgtcgt
     2161 tcctgctggt cacccacgac ctcggcgtgg ccgcccaggt cgccgaccac atcgccgttc
     2221 tgtacgccgg ccggctggcc gagatcggcg ccagcgacga cgtgctgcgg cgcccggccc
     2281 acccgtacag ccgcggcctg ctgcagtccc ggctcaccct gacggctcgg cgggaggggc
     2341 cgctccccac cctgcccggc gagccccccg acccacgcgc ccagccgccg gggtgcccct
     2401 tcgccccccg ctgtgacctc cgcgaggacc gctgcgagga ggagctcccc gacctggagc
     2461 cctccggccg ccatcccggg tgggcggcgt gcctgc
//
LOCUS       DASWGG010000214         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3109219, whole genome shotgun sequence.
ACCESSION   DASWGG010000214 DASWGG010000000
VERSION     DASWGG010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3109219"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VF954_03875"
     CDS             complement(<1..200)
                     /locus_tag="VF954_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9530253.1"
                     /translation="MSQKPVSQKPVSQKPLSQKPVSQKRSLLQGLYVISVAAELAGVH
                     PQTLRIYERKGLVEPQRTVGRSR"
     gene            complement(206..1318)
                     /gene="dnaJ"
                     /locus_tag="VF954_03880"
     CDS             complement(206..1318)
                     /gene="dnaJ"
                     /locus_tag="VF954_03880"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX9530254.1"
                     /translation="MAPQREWFEKDYYQVLGVSGTATEKEITRAYRKLAKQYHPDANP
                     GSEDRFKDISAAYDVLGDPARRKEYDEVRRLGPAGGMFGGGGPGGDGAGPFTNVRVED
                     LSDLLGGMFRGGGRTRTRSTSGPRRGEDLEAELYLSFEDAIDGVTTTINVVSEVACAT
                     CHGTGAAPGTSPVICSQCGGRGVTADNQGMFSFSSPCRACAGTGMRVETPCPTCQGTG
                     NTRRPRQVKTKIQPGVEDGKRLRLKARGGSGHNGGPPGDLYVTVHVGRHDLFGRRGKD
                     LLVAVPITFAEAALGATVTVPTLDKPVKVKIPPGTRSGRTFRVRGHGVPDPKGAGDLL
                     ATVEVAVPQHLSDAERKAIEDLRAASDGSPRSHLGV"
     gene            complement(1324..2040)
                     /gene="grpE"
                     /locus_tag="VF954_03885"
     CDS             complement(1324..2040)
                     /gene="grpE"
                     /locus_tag="VF954_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX9530255.1"
                     /translation="MSGEGHAVGTDDDFVAQPHVEAHVVDAPTGDEAAAPRDEAAAPP
                     DAVVPDELPADEAPQGEAPPPPEGDPDEGGTDEGGTVEGDLAELTVRVQGERDEYLDA
                     LRRLQADFANYKKRVLKHQSEQAERAAELLVVKLLPVLDTVDLAVAHTVEGDGPGEAG
                     ALQQVAASLTDTLAKEGLERIDPVGQAFDPMEADAVVHEPGQPGEGGGDDGPIVTGVL
                     RAGYRWKGRVVRPAMVKVTG"
     gene            complement(2037..>3913)
                     /gene="dnaK"
                     /locus_tag="VF954_03890"
     CDS             complement(2037..>3913)
                     /gene="dnaK"
                     /locus_tag="VF954_03890"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX9530256.1"
                     /translation="RLVPMPKAVGIDLGTTNSVVAVLEAGEPVVIPNAEGARTTPSIV
                     GFSKTGEVLVGEVAKRQAITNPDRTIRSVKRHMGETSWSVAIDAKKYSAQEISARVLQ
                     KLKRDAEAYLGDTVNQAVITVPAYFDDAQRTATKEAGQIAGLEVLRIINEPTAAALAY
                     GMDKEGADQTVLVFDLGGGTFDVSVLEIGEGVFEVKSTSGNTHLGGDDWDQRVIDWLV
                     KEFKNTEGVDLGVDKMALQRLKEAGEKAKIELSSVQETTINLPFITATSEGPKHLDLK
                     LTRAKFNELTEDLVQACKGPFEQAIKDAGMTRDDIHHVIMVGGSTRMPAVQELVQSMT
                     GEEPHKGVNPDEVVAVGAAIQAGVLKGEVKDVLLLDVTPLSLGIETKGGIMTKLIERN
                     TTIPTRRTEVFTTAEDMQPSVEIHVLQGEREMAMYNKTLGKFQLVDLPPAPRGLPQIE
                     VTFDIDANGIVHVSAKDRATSKEQSMTITGQSSLNKDDIERMVRDAEAHAEDDRNRRE
                     EAEVRNNADTLVYQTEKLLKEQGDKFSGSDKESVEAALTGVKEALSGSDVEAIKRATE
                     SLMTASQGFAQKLYEQAATDPAGGAAGAGGPGGPSGDGASAPRDDEVVDAEIVDEHGA
                     "
BASE COUNT          448 a         1420 c         1371 g          674 t
ORIGIN      
        1 cgggaccggc ccaccgtccg ctgcggctcg accaggccct tccgctcgta gatccgcagg
       61 gtctgggggt gcaccccggc cagctcggcc gccaccgaga tgacgtacag gccctggagc
      121 aggctgcgtt tctgggacac gggcttctgg gacaagggct tctgggacac gggcttctgg
      181 gacacgggct tctgggacat ggtgctcaca cccccaggtg cgatcggggc gagccgtccg
      241 acgccgcccg gaggtcctcg atggccttgc gctcggcgtc ggacaggtgc tggggcacgg
      301 ccacctcgac ggtggccagc aggtcgccgg cgcccttggg gtcggggacg ccgtggcccc
      361 ggacccggaa ggtgcggccc gagcgggtgc cgggcgggat cttcaccttc accggcttgt
      421 ccagggtggg gaccgtgacg gtggcgccga gggcggcctc ggcgaaggtg atcggcaccg
      481 cgacgagcag gtccttgccc cgccggccga acaggtcgtg gcggcccacg tgcaccgtca
      541 catagagatc gcccgggggc ccgccgttgt ggcccgaccc gccccgggcc ttgagccgca
      601 ggcgcttccc gtcctccacg cccggctgga tcttggtctt gacctgccgg ggccggcggg
      661 tgttgcccgt gccttggcac gtggggcacg gggtctcgac acgcatgccg gtgccggcgc
      721 acgcccggca cggggaggag aacgagaaca tgccctggtt gtccgcggtg acgccccgcc
      781 cgccgcactg cgagcagatg acggggctgg tgcccggcgc cgcgcccgtc ccgtggcagg
      841 tggcgcacgc cacctcgctc accacgttga tggtcgtggt caccccgtcg atggcgtcct
      901 cgaaggacag gtacagctcg gcttcgaggt cctcgccccg ccggggaccg ctggtcgagc
      961 gggtccgggt ccggcccccg ccccggaaca tgccgccgag caggtcgctg aggtcctcga
     1021 cccgcacgtt ggtgaacggc ccggccccgt cgccgcccgg gccgcccccg ccgaacatgc
     1081 cgcccgccgg cccaagccgg cggacctcgt cgtactcctt gcgcctggcc gggtcgccca
     1141 gcacgtcgta ggccgcggag atgtccttga agcggtcctc ggaaccgggg ttggcgtccg
     1201 ggtggtactg cttggccagc ttccggtagg cgcgggtgat ctccttctcg gtggccgtgc
     1261 ccgacacccc gaggacctgg taatagtcct tctcgaacca ctcccgctgg ggcgccatct
     1321 cgtttacccc gtgactttca ccatggcggg tcgcacgacg cggcccttcc accggtagcc
     1381 ggcccgcagc acgcccgtga cgatcgggcc gtcgtcgccg ccgccctcgc ccggctgtcc
     1441 cggctcgtgc acgacggcgt cggcctccat cgggtcgaag gcctggccca cggggtcgat
     1501 gcgctcgagc ccctccttgg ccagggtgtc ggtgaggctg gccgccacct gctggagcgc
     1561 cccggcctcg ccggggccgt cgccctcgac ggtgtgggcc acggcgaggt cgaccgtgtc
     1621 gagcaccggc agcagcttga cgacgagcag ctcggcggcc cgctcggcct gctccgactg
     1681 gtgcttgagc acccgcttct tgtagttggc gaagtcggcc tggaggcggc gcagggcgtc
     1741 gaggtactcg tctcgctcgc cctggacccg caccgtcagc tcggccaggt cgccctccac
     1801 ggtgccgccc tcgtcggtgc cgccctcgtc ggggtcccct tccgggggcg ggggcgcctc
     1861 gccctggggc gcctcgtccg caggcaactc gtccgggacc accgcgtccg ggggcgcggc
     1921 tgcctcgtcc cggggcgcgg ccgcctcgtc cccggtcggg gcgtcgacca cgtgggcctc
     1981 cacgtgcggc tgggctacga agtcgtcgtc ggtgcccacc gcgtgcccct ccccactcac
     2041 gccccgtgct cgtcgacgat ctcggcgtcg acgacctcgt cgtcacgggg ggcgctggcg
     2101 ccgtcgcccg acgggccacc gggcccgccc gcgccggccg ccccgccggc cgggtccgtg
     2161 gccgcctgct cgtacagctt ctgggcgaag ccctggctgg cggtcatcag gctctcggtg
     2221 gccctcttga tggcctcgac gtcgctgccg gacaaggcct ccttgacgcc cgtcaacgcc
     2281 gcctccaccg actccttgtc cgacccggag aacttgtcgc cctgctcctt cagcagcttc
     2341 tccgtctggt acacgagggt gtcggcattg ttgcggacct cggcctcctc ccggcggttg
     2401 cggtcgtcct cggcatgggc ctcggcgtcg cgcaccatgc gctcgatgtc gtccttgttg
     2461 agcgaggact ggccggtgat ggtcatcgac tgctccttgc tcgtggcccg gtccttggcc
     2521 gacacgtgca cgatgccgtt ggcgtcgatg tcgaaggtga cctcgatctg cggcaggccc
     2581 cgcggcgcgg ggggcaggtc gaccagctgg aacttgccga gagtcttgtt gtacatggcc
     2641 atctcccgct cgccctggag cacgtggatc tccaccgacg gctgcatgtc ctcggccgtc
     2701 gtgaacacct ccgtgcggcg ggtcgggatc gtcgtgttgc gctcgatcag cttggtcatg
     2761 atgccgccct tggtctctat gccgaggctc agcggggtga cgtcgaggag caggacgtcc
     2821 ttgacctcgc ccttgagcac gccggcctgg atggcggcgc ccaccgccac gacctcgtcg
     2881 gggttgacgc ccttgtgggg ctcctcgccg gtcatggact ggacgagctc ctgcaccgcc
     2941 ggcatccggg tggagccgcc caccatgatg acgtggtgga tgtcgtcgcg ggtcatgccg
     3001 gcgtccttga tggcctgctc gaacggtccc ttgcacgcct gcaccaggtc ctcggtcagc
     3061 tcgttgaact tggcccgggt cagcttcagg tccaggtgct tcgggccctc ggaggtggcg
     3121 gtgatgaacg gcaggttgat ggtcgtctcc tgaacggagg acagctcgat cttggccttc
     3181 tcgcccgctt ccttcagccg ctggagcgcc atcttgtcca cgcccaggtc gacgccctcg
     3241 gtgttcttga actccttgac cagccagtcg atgacccgct ggtcccagtc gtcgccgccc
     3301 aggtgggtgt tgccggacgt ggacttgacc tcgaagacgc cctcgccgat ctcgagcacc
     3361 gacacgtcga aggtgccgcc gcccaggtcg aacaccagca ccgtctggtc ggcgccctcc
     3421 ttgtccatcc cgtaggcgag ggcggccgcg gtgggctcgt tgatgatgcg cagcacctcg
     3481 agcccggcga tctggccggc ctccttggtg gcggtgcgct gggcgtcgtc gaagtacgcc
     3541 ggcacggtga tcacggcctg gttcacggtg tcgcccaggt aggcctcggc gtccctcttg
     3601 agcttctgca gcacgcgggc ggagatctcc tgggccgagt acttcttggc gtcgatggcc
     3661 accgaccacg acgtctcgcc catgtggcgc ttgaccgagc ggatcgtgcg gtccgggttg
     3721 gtgatcgcct ggcgcttggc cacctcgccg acgagcacct cgcctgtctt ggagaagccc
     3781 acgatcgacg gggtggtccg ggccccctcg gcgttgggga tgacgacggg ctcgcccgcc
     3841 tccaggacgg ccacgacgga gttggtggtg ccgagatcga tgcctacggc cttgggcatg
     3901 ggaactagcc tcc
//
LOCUS       DASWGG010000215         1720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1824502, whole genome shotgun sequence.
ACCESSION   DASWGG010000215 DASWGG010000000
VERSION     DASWGG010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824502"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VF954_03895"
     CDS             complement(<1..312)
                     /locus_tag="VF954_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX9530257.1"
                     /translation="MALRLRARRKEKPIEGRMTLVEHLAELRRRLVVCALAVGAGATV
                     GFFLYGHILALLVHPYCRVLPRAALPPGGRCTGLLLVTDPLEPFVIRLKIAGWAGLFF
                     AS"
     gene            complement(319..672)
                     /locus_tag="VF954_03900"
     CDS             complement(319..672)
                     /locus_tag="VF954_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX9530258.1"
                     /translation="MLNLGPEKVLVVLVVALIVLGPEKLPQAARTVGTAYRELRRITG
                     GLQAEVRDVFSEPLREMRSAFDEPAFDEPAFDEPVAPGAAPESDATHEPAGASPPPPP
                     GPSPVPFRPGDPSLN"
     gene            complement(719..925)
                     /locus_tag="VF954_03905"
     CDS             complement(719..925)
                     /locus_tag="VF954_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530259.1"
                     /translation="MSMLWWLVPTCVAAGLAVPAVVAALRAADEARALGRAVTDLGSL
                     RPALVEVRSGADAVRQHLLDLRSR"
     gene            complement(922..1263)
                     /locus_tag="VF954_03910"
     CDS             complement(922..1263)
                     /locus_tag="VF954_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530260.1"
                     /translation="MATIRASCPTCGDVELTTRDVRVLVCSTTNEGSYAFRCPGCRVQ
                     VSKPAEGRIVDVLVSSGVRLSVWHLPAELDEPHSGPPISYDDLLEFHYELQGEDWFDR
                     VTAKVTGADGP"
     gene            1372..>1720
                     /locus_tag="VF954_03915"
     CDS             1372..>1720
                     /locus_tag="VF954_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530261.1"
                     /translation="MPVPASSHLERLQALTRSFAAGVDSARLLQEAAAAAASGSCAAA
                     TLVVRLSAGQPLPVAVSGVPPEVLMDVVGEAVRAGRLVHRRLAPPDAAAGKRLGRGPV
                     AAAIPLRVDSRVIG"
BASE COUNT          232 a          608 c          656 g          224 t
ORIGIN      
        1 cgaggcgaag aacagtcccg cccagccggc gatcttgaga cggatcacga agggctcgag
       61 agggtccgtc acgagcagca gcccggtgca cctcccgccc ggaggcaggg cggcgcgagg
      121 cagcacccgg cagtacgggt ggacgagcag ggcgaggatg tggccgtaga ggaagaaccc
      181 gacggtcgct ccggccccga cggcgagggc gcagaccacc agccgccgcc gcagctcggc
      241 caggtgctcc acgagggtca tgcgcccctc gatcggcttt tccttgcgcc gggctcgcag
      301 tcggagggcc acggtcgctc agttcaagct ggggtcgccc gggcggaacg gcaccgggct
      361 cgggccgggc ggcgggggcg gcgaggcccc ggcgggctca tgggtcgcgt cgctctcggg
      421 cgccgcgccc ggcgccaccg gctcgtcaaa ggccggctcg tcaaaggccg gctcgtcgaa
      481 ggcgctgcgc atctcgcgga gcggctcgga gaagacgtcg cggacctcgg cctggagccc
      541 gccggtgatg cgcctcagct ctcggtacgc ggtgccgacc gttcgggccg cctgggggag
      601 cttctccggc cccagcacga tcagggcgac cacgaggacc acgaggacct tctcggggcc
      661 gaggttcagc acgccccgaa gtctaccgac ggggctgcct aacgagcgcg gccgggcgtc
      721 atcgggagcg caggtcgagc aggtgctggc ggaccgcgtc cgccccggag cggacctcca
      781 ccagcgccgg gcgaaggctg cccaggtcgg tcacggcccg acccagggcg cgggcctcgt
      841 cggcggcccg cagggcggcc acgaccgcag gtacggcgag cccggccgcc acgcaggtgg
      901 gcaccagcca ccacagcatg ctcatggtcc gtctgcccct gtcaccttgg ccgtcacccg
      961 gtcgaaccag tcctcgcctt gaagctcgta gtggaactcc aggaggtcat cgtaggagat
     1021 cggggggccg gaatggggct cgtccagctc ggcgggcagg tgccagacgg acagccggac
     1081 cccggaggag accaggacgt ccacgatgcg gccctcggcg ggcttggaga cctggacgcg
     1141 gcagccgggg cagcggaagg cgtaggaccc ctcgttggtg gtggagcaca ccaggacgcg
     1201 gacgtctcgg gtggtcagct cgacatctcc gcaggtggga caactcgccc ggatcgtggc
     1261 catcaccgtc tccttgtcgg ccggtacctg cgtctcatcg gcgcctgtgg gcgtcaagtt
     1321 gagcgcccgg cggctttagc cgtgccccta tggctccgat acgggaggag catgcccgta
     1381 cctgcctctt cgcatctcga gcggctccag gccctgaccc gctcgttcgc ggccggcgtg
     1441 gacagcgccc gcctgctcca ggaggcggcc gcggcggcgg cttcgggctc gtgcgccgcc
     1501 gccaccctgg tggtccggct gtcggcaggg cagccattgc ccgtggcggt gtcgggcgtg
     1561 ccccctgagg tgctcatgga cgtggtgggc gaggccgttc gggccggtcg actggtccac
     1621 cgccggctgg cgcctccgga cgcggccgcc ggcaagcggc tgggccgggg gccggtcgcg
     1681 gccgccatcc cgctgcgagt cgactcccgg gtgatcggtg
//
LOCUS       DASWGG010000216         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2355582, whole genome shotgun sequence.
ACCESSION   DASWGG010000216 DASWGG010000000
VERSION     DASWGG010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2355582"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VF954_03920"
     CDS             <1..457
                     /locus_tag="VF954_03920"
                     /inference="COORDINATES: protein motif:HMM:NF021926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HEX9530262.1"
                     /translation="QSTLSAEEVALINQGGLHLGLVIDEHKVEFGPGDFLVRNVMGAD
                     QDNGALQIGAEVDVGATVQFHLRDAAAADADLHAVLDGATADAALLFTCNGRGRRLFG
                     RPGHDAGALAEAVGPVPTAGLFCAGEFGPVSGRNFLHGFTASIALFSRR"
     gene            498..>2349
                     /gene="tkt"
                     /locus_tag="VF954_03925"
     CDS             498..>2349
                     /gene="tkt"
                     /locus_tag="VF954_03925"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX9530263.1"
                     /translation="MSDLDLTQLGINVIRGLAMDAPQKANSGHPGTAMALAPLAHVLW
                     TRVMRHDPAAPDWPDRDRFVLSCGHASILLYSMLYLTGYGPELEDLRQFRQWGSRTPG
                     HPEVRDLPGIEVTTGPLGQGVANGVGMGIAERRLRAHFGPEVCDHHTFVLCSDGDLEE
                     GLSHEAASLAGHLGLGRLVYVYDDNHITIDGPTELAFSDDTPARFEAYGWHVERVGEI
                     ANDTDGLEAALRRAMAERDRPSLIVLRSHIGWPSPTFTDTKEAHGNPLGEDEVRRTKE
                     ILGLPPDKTFWVPDEVLRMYRECIPRGQAMRRAWEGRFEAWSGDRQEWEAAQAGRGVP
                     GWAEKLPTWKAGEQIATRQAIHACLNAVKDLVPGLMSGGADLTGNTGTALDDEPLQSR
                     DHPEGRVMAYGIREHGMGGVMNGMAMHGGVVPVGGTFFCFSDYMRPSVRLAALSGAHV
                     VYSWTHDSIGLGEDGPTHQPVEQLAAMRAMPNLSVIRPADANETAAAVRLAVDGDGPT
                     GLVLSRQKLPVLEGTADHLDDVARGAYVLAEPAGDPELILIGTGSEVALCMGAADRLG
                     ARGLPARVVSMPSWDLFEAQTDAYRDTVLPPELPALAVEAASPFGWDRWAD"
BASE COUNT          323 a          885 c          840 g          301 t
ORIGIN      
        1 ccagtccacg ctcagcgcgg aggaggtcgc cctcatcaac cagggcgggc tccacctggg
       61 gctggtgatc gacgagcaca aggtggagtt cgggccgggc gacttcctcg tccgcaacgt
      121 gatgggggcc gaccaggaca acggcgccct ccagatcggc gccgaggtcg acgtgggcgc
      181 aaccgtgcag ttccacctcc gcgacgccgc cgcggccgac gccgacctgc acgccgtgct
      241 cgacggcgcc accgccgacg ccgccctgct cttcacctgc aacggccggg gccggcgcct
      301 gttcgggcgg cccggtcacg acgccggcgc cctggccgag gcggtgggcc ccgtcccgac
      361 agccgggctg ttctgcgccg gggagttcgg gcccgtgtcc gggcgcaact tcctgcacgg
      421 cttcaccgcg tcgatcgcgc tgttcagccg gcgctagggc cgtcggagcg accggcatcg
      481 tgggtagggt gctggcaatg agcgacctgg acctcaccca gctcgggatc aacgtcatcc
      541 ggggcctcgc catggacgcc ccccagaagg ccaacagcgg ccaccccggc acggccatgg
      601 ccctggcacc gctggcgcac gtgctgtgga cgagggtgat gcgccacgac ccggccgcgc
      661 ccgactggcc cgaccgggac cgcttcgtgc tgtcctgcgg ccacgccagc atcctgctgt
      721 actcgatgct gtacctgacc ggctacggcc ccgagctcga ggacctccgc cagtttcgcc
      781 agtggggcag tcgcacgccg ggccatccgg aggtccggga cctgccgggg atcgaggtca
      841 cgactggccc gctggggcag ggggtggcaa acggggtcgg gatgggcatc gccgagcggc
      901 ggctgcgggc gcacttcggg ccggaggtct gcgaccacca cacgttcgta ttgtgctccg
      961 acggcgacct ggaagagggc ttgagccacg aggcggcgtc gctcgccggg catctgggcc
     1021 tgggccggct cgtgtacgtc tacgacgaca accacatcac catcgacggt ccgaccgagc
     1081 tggccttcag cgacgacacc ccggcgcgct tcgaggcgta cggctggcac gtcgagcggg
     1141 tgggcgagat cgccaacgac accgacggcc tggaggccgc cctccgccgg gccatggccg
     1201 agcgggaccg gccctcgctc atcgtgctcc gcagccacat cggctggccc tcgccgacct
     1261 tcaccgacac caaggaggcg cacggcaacc ccctcggcga ggacgaggtc cgccgcacca
     1321 aggagatcct gggcctgccc ccggacaaga cgttctgggt ccccgacgag gtcctccgca
     1381 tgtaccgcga gtgcatcccc cgcgggcagg ccatgcggcg ggcctgggag gggcgcttcg
     1441 aggcctggtc cggcgaccgg caggagtggg aggccgccca ggccgggcgg ggggtcccgg
     1501 gctgggcgga gaagctgccc acctggaagg cgggcgagca gatcgcgacc cgccaggcca
     1561 tccacgcttg cctcaacgcc gtcaaggacc tggtcccggg actgatgtcc gggggcgccg
     1621 atctcaccgg caacaccggc accgcgctcg acgacgagcc gctccagtcg cgggaccacc
     1681 cggagggccg ggtcatggcc tacggcatcc gcgagcacgg catgggcggg gtcatgaacg
     1741 gcatggccat gcacggcggc gtcgtgcccg tcggcggcac cttcttctgc ttcagcgact
     1801 acatgcggcc ctcggtccgc ctggccgcgc tgtccggtgc ccacgtcgtg tactcctgga
     1861 cccacgactc catcggcctc ggcgaggacg ggcccaccca ccagcccgtc gagcagctgg
     1921 cggccatgcg ggccatgccc aacctgagcg tcatccgccc ggccgacgcc aacgagacgg
     1981 cggctgcggt gcggctggcc gtcgacggcg acggcccgac cggcctggtc ctcagccgcc
     2041 agaagctgcc cgtgctggag ggcacggccg accacctcga cgacgtcgcc aggggcgcct
     2101 acgtgctcgc cgagcccgcc ggcgaccccg agctgatcct gatcggcacg ggctcggagg
     2161 tggcgctgtg catgggcgcg gccgatcgcc tcggcgcccg gggcctcccc gcccgggtgg
     2221 tgtccatgcc gagctgggac cttttcgagg cccagaccga cgcctaccgc gacaccgtgc
     2281 tcccgcccga gctgcccgcc ctcgccgtgg aggccgcctc ccccttcggc tgggaccggt
     2341 gggcggacg
//
LOCUS       DASWGG010000217         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1182914, whole genome shotgun sequence.
ACCESSION   DASWGG010000217 DASWGG010000000
VERSION     DASWGG010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182914"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VF954_03930"
     CDS             complement(<1..221)
                     /locus_tag="VF954_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX9530264.1"
                     /translation="MPEQSGSEPPVVSFLSDYGLTDEFVGVCKAVVLRAAPSAQIIDV
                     THGIPPFDVRAGALALARSVQYLPPGVVLA"
     gene            complement(381..581)
                     /locus_tag="VF954_03935"
     CDS             complement(381..581)
                     /locus_tag="VF954_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9530265.1"
                     /translation="MAGGDRGDRVAQSFEKARFLTVQEVAQLMRVSSMTVYRLIRAGD
                     LPAVRVGRSFRVRDVDVDGYLQ"
     gene            complement(737..1540)
                     /gene="proC"
                     /locus_tag="VF954_03940"
     CDS             complement(737..1540)
                     /gene="proC"
                     /locus_tag="VF954_03940"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX9530266.1"
                     /translation="MTTLALIGGGKMGEALLGGLVDGGWDPEHLAVAEVSAERRHAIE
                     GRFPKVRVVPNPAWAVAEAEVVVVAVKPGDVAAALEMAEPVLEPGALVLSIAAGVTIA
                     ALEAVVPGRPVVRAMPNTPALVGLGAAAIAAGRHAGEEHLARAESILSAVGTVARVPE
                     SLLDAVTGLSGSGPAYIFLVAEALIEAGVHVGLPRPVSQALVTQTLLGSATLLAQGPD
                     GPEALRAAVTSPGGTTAAGVAALESRAVRAAFLEAVAAATARSRELGEN"
     gene            complement(1754..2203)
                     /locus_tag="VF954_03945"
     CDS             complement(1754..2203)
                     /locus_tag="VF954_03945"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX9530267.1"
                     /translation="MKRDEAADLVRDHLTGPVAAQPWVQHVEHDPELDRWYLRFGCDG
                     RDATTVYFDLRPRSLRYEVYFLPDPSGASPAPSEGKRLELYRWLLRRNHHEPYNARFS
                     IGPDGDIYLTGRVLYQHLDEEELDRLIGVCYETVETWFQTAVTLGFR"
BASE COUNT          370 a          790 c          804 g          311 t
ORIGIN      
        1 gccaagacca ccccgggcgg gaggtactgg accgatcgcg ccagcgccaa agcccccgcc
       61 cgaacgtcga agggcgggat gccgtgggtg acatcgatga tctgggccga cggcgcggcc
      121 ctcaggacca cggccttgca cacaccgacg aattcgtcgg tcaggccgta gtcactgagg
      181 aacgagacga caggaggttc tgaacctgac tgttcgggca cggcgcgctc cctgagtact
      241 cagaattcat tccacatcag tgggaacagc atgaattctg agtattcatc agcggggtga
      301 atgacccgtc cgacgctagc ggccggacgg gcccggaagc ggaagccgcg cgctggtgct
      361 cagccggcct gggtgtaccg ctactggaga tagccgtcga cgtcgacgtc ccggacccgg
      421 aaagagcgcc cgacccgcac cgcgggaagg tcgccagccc ggatgagccg gtagacggtc
      481 atcgacgaaa ccctcattag ttgcgcgact tcttgaaccg tcaagaagcg cgccttctca
      541 aaagactgcg ccacgcggtc acccctatct ccacccgcca ccttcgaggg tacgccctgt
      601 tagggcagtt gtctagggcg ggggttgcgc cacatgagcg atttcgcgtc gcagagacgt
      661 ggcacgacca caatgcgtga aaccgttacc gtcagttgcc acgacgcgcc gtgatcactc
      721 cggcgccacg ggaagctcaa ttttcgccca attctcggga acgggcggtg gccgccgcca
      781 ccgcctccag gaaggcggcc cgcacggccc gggactccag ggcggccaca ccggcggccg
      841 tggtcccccc gggggaggtg accgccgccc ggagggcctc cgggccgtcg gggccctggg
      901 ccaggagggt ggccgacccg aggagggtct gggtgaccag cgcctggctg accggccggg
      961 ggaggccgac gtggacgccg gcttcgatga gcgcctcggc caccaggaag atgtaggccg
     1021 ggcccgaccc cgagaggccg gtgacggcgt ccaacaggct ctcggggacc cgggccaccg
     1081 tccccacggc cgagaggatc gactccgccc gggccaggtg ctcctctccg gcgtggcgcc
     1141 cggcggcgat ggcggccgcc cccagcccga ccagcgccgg cgtgttcggc atggcccgga
     1201 caaccggccg gccgggtacc accgcctcga gggcggcgat ggtgacgccg gcggcgatcg
     1261 acaggacgag agcacccggt tcgagcaccg gctccgccat ctccagcgcc gcggccacgt
     1321 cgcccggctt cacggccacc accacgacct cggcttcggc caccgcccag gccgggttgg
     1381 gcacgacccg caccttgggg aagcggccct cgatggcgtg gcgccgctcg gcgctgactt
     1441 cggccacggc caggtgctcc ggatcccagc caccgtcgac gagacctccc aggagtgcct
     1501 cgcccatctt gccgccgccg atcagagcca gtgtcgtcac gcagcgaggg tacttcgcgg
     1561 ccgggggcgc cccgccgcca gaagcacaga cgcgggcggg cccgaggaac cattggtgtt
     1621 actccgcact tccccgaacg gagtagcccc gcctgcacct cgggcctcgc ctggaccgcc
     1681 acccgtcggt tgcggcgagg tgcaccctaa cccgccatct cctttgctgg caaaggattt
     1741 ttcgccgagt gacctagcgg aaacccagag tgacggcggt ctggaaccac gtctcgacgg
     1801 tctcgtagca gacaccgatc aggcggtcga gctcttcctc gtcgaggtgc tggtagagca
     1861 cccggccggt caggtagatg tcgccgtcgg gcccgatcga gaagcgggcg ttgtagggct
     1921 cgtggtggtt gcggcgcagc agccaccggt agagctcgag gcgtttgccc tcggaggggg
     1981 caggtgaagc ccccgaaggg tcgggcagga agtagacctc gtaccgcagc gagcgggggc
     2041 ggaggtcgaa gtagaccgtg gtggcgtcgc gcccgtcgca gccgaagcgg aggtaccacc
     2101 ggtcgagttc ggggtcgtgc tcgacgtgct gcacccaggg ctgggccgcc accgggcccg
     2161 tgaggtggtc ccgaacgaga tcggctgcct cgtcgcgttt caccgcgggc tcagctgcag
     2221 gtgaccggcg tgcgctccac caggtcgctg cagaggcgcc ggaggcgggc ggcgg
//
LOCUS       DASWGG010000218         2476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_422555, whole genome shotgun sequence.
ACCESSION   DASWGG010000218 DASWGG010000000
VERSION     DASWGG010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422555"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VF954_03950"
     CDS             <1..816
                     /locus_tag="VF954_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX9530268.1"
                     /translation="GIVAVVTNVSVDHAEIIGPTLADIAIEKAGIVKPGATLVLGERS
                     PELAPVFLDTPAGQVWARFDDFDLLDNRVAHGGRLLDLRTPAAAYHDMFLGLHGPYQG
                     ENAAVALAAAEAFFAQPLSEEVVAEAFAAVRSPGRMEVVGRKPLTIIDGAHNPAGAHA
                     LAAALDEEFGAVDGRVVLIGLLSGRDPDEMLEPLLEPGDTRLVVACAPPSPRAMAPLE
                     IVGAATRLGVAAVAAREPAEALRLARQAVGEEELLLVTGSLYLVGALRSLVRG"
     gene            828..1241
                     /gene="ndk"
                     /locus_tag="VF954_03955"
     CDS             828..1241
                     /gene="ndk"
                     /locus_tag="VF954_03955"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX9530269.1"
                     /translation="MDRTLVICKPDAVARGLAGEIVARLERRGLRLVAAELRTIDADT
                     AGRHYAEHQGKPFYGELVSFITRSPSLVLVVEGPDGTWSMVRTMMGPTNPADAPPGTI
                     RGDLGVVLTENLVHGSDSAASAEREIDIFFPAVGL"
     gene            1304..2341
                     /locus_tag="VF954_03960"
     CDS             1304..2341
                     /locus_tag="VF954_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX9530270.1"
                     /translation="MSDNFLSSFLGRDMAVDLGTANTLVYVRGRGIVLNEPSVVAVNV
                     KDGRPLAVGAEAKRMIGRTPHHIQAIRPLKDGVIADFEICEKMLRYFIHRVHQRRFAK
                     PRMVICVPSGITGVEQRAVQEAAEYAGARKPAYIIEEPMAAAIGAGLPVHEPTGNMVV
                     DIGGGTTEVAVISLGGIVTSASIRIGGDELDEAIIQFIKKEYSLALGERTSEEIKIAL
                     GSACPLEEELHAEIRGRDLITGLPKTIVTTTEEIREAIEEPVTAIVDAVKVTLDKTPP
                     ELAADIMEQGIVLTGGGALLHGLDARLSAETGMPIVIAKNPLHSVAIGSGQCLEEFEA
                     LKQVLISSSSH"
BASE COUNT          365 a          887 c          862 g          362 t
ORIGIN      
        1 gggatcgtgg ccgtggtgac caacgtcagc gtcgaccacg ccgagatcat cgggccgacg
       61 ctcgccgaca tcgcgatcga gaaggccggc atcgtcaaac cgggcgccac gcttgtcctc
      121 ggcgagcgca gcccggagct ggcgcccgtc ttcctcgaca cgccggcggg acaggtctgg
      181 gcccggttcg acgacttcga cctcctcgac aaccgggtgg cccatggggg ccgtctgctc
      241 gacctgcgca ctccggcggc ggcctaccac gacatgttcc tgggcctgca cggcccgtac
      301 cagggagaga acgcagccgt tgcgctggcc gccgccgagg cgttcttcgc ccagccgctc
      361 tcggaggagg tcgtggccga ggccttcgcg gccgtccgct cgcccggccg gatggaggtg
      421 gtcgggcgca agcccctgac catcatcgac ggggcccaca accccgccgg cgcccacgcc
      481 ctggcggccg cgctggacga ggagttcggc gccgtggacg ggcgggtggt gctcatcggg
      541 ctgctcagcg ggcgcgaccc cgacgagatg ctggagcccc tgctcgagcc gggcgacacc
      601 cgcctcgtgg tggcctgcgc cccgccatcg ccgcgggcca tggcgccgct cgagatagtc
      661 ggggccgcca cccggctcgg ggtggcggcg gtcgcggccc gcgagccggc cgaggcgctg
      721 cgcctggcgc gccaggcggt tggcgaggag gagctgctgc tggtcacggg gtccctctac
      781 ctggtcggag ccctccgctc gctggtccgc gggtagcgtc agccgccatg gaccggacac
      841 tggtcatctg caagcccgat gccgtcgcgc gcgggctggc cggcgagatc gtcgcccgcc
      901 tcgagcgcag aggtctgcgc ctcgtcgccg ccgagctccg cacgatcgac gcggatacgg
      961 ccgggcggca ttacgccgag caccagggca agccgttcta cggcgagctg gtcagcttca
     1021 tcacccggtc gccctccctg gtcctggtgg tcgaggggcc cgacggcacc tggagcatgg
     1081 tgcgcaccat gatgggcccc accaaccccg ccgacgcccc gcctggcacc atccgcggcg
     1141 acctgggtgt cgtgctcaca gagaacctgg tgcacggatc ggactcggcc gcctcggcgg
     1201 agcgcgagat cgacatcttc ttcccggccg tcggcttgta gcggccgcaa ccctccccta
     1261 gcctgacggg tagacccccg cctatcctcg agaaagcgct ggcgtgtccg ataacttcct
     1321 gtcttctttt ctgggccgcg acatggctgt cgacctcggc acggcgaaca ctctggtgta
     1381 cgtccggggt cgcggcatcg tgctcaacga gccgtcggtg gtcgccgtca acgtcaagga
     1441 cggccggccg ttggccgtgg gcgccgaggc caagcggatg atcgggcgca cgccgcacca
     1501 catccaggcc atccgccccc tgaaggacgg cgtcatcgcc gacttcgaga tctgcgagaa
     1561 gatgctgcgc tacttcatcc accgggtgca ccagcgccgc ttcgccaagc cccgcatggt
     1621 gatctgcgtg ccgtcgggca tcaccggcgt ggagcagcgg gccgtgcagg aagccgccga
     1681 gtacgccggg gcgcgcaagc cggcctacat catcgaagag cccatggcgg cggccatcgg
     1741 cgccggcctg ccggtgcacg agcccacggg caacatggtc gtcgacatcg gcggcggcac
     1801 gaccgaggtg gccgtgatct cgctcggggg aatcgtcacc agcgcgtcga tccgcatcgg
     1861 cggagacgag ctggacgagg ccatcatcca gttcatcaag aaggagtaca gcctggccct
     1921 gggcgagcgc acgtcggagg agatcaagat cgccctcggc tctgcctgcc cgctcgagga
     1981 ggagctgcac gccgagatca ggggccggga cctcatcacc ggactgccga agacgatcgt
     2041 gaccaccacc gaggagatcc gggaggccat cgaggagccc gtcacggcca tcgtcgacgc
     2101 cgtcaaggtg acgctcgaca agacccctcc ggagctggcg gccgacatca tggagcaggg
     2161 gatcgtcctg accggcggcg gcgcgctgct gcacggcctc gacgcccggc tgtcggccga
     2221 gacggggatg cccatcgtca tcgccaagaa cccgctgcac tccgttgcca tcgggagcgg
     2281 ccagtgcctc gaagagttcg aggccttgaa gcaggtgctg atctcttcga gctcgcacta
     2341 gaagatctgt ggccgtctac cggcgctctc atcgcacccg cttcgtgctg ctgctgctgg
     2401 tcctgtcgtc agtcaccctg atcaccgtcg gctaccgcgg cggccgggca tcgagcgcgc
     2461 tggagtcggt acgggg
//
LOCUS       DASWGG010000219         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_855343, whole genome shotgun sequence.
ACCESSION   DASWGG010000219 DASWGG010000000
VERSION     DASWGG010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855343"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VF954_03965"
     CDS             <1..754
                     /locus_tag="VF954_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530271.1"
                     /translation="TVRFRFAFLDAGPAGCRPTAPATAAFLAIPGTPVPSPPPARAQL
                     TSSASGEGVYGAPAGFDRAGNWEVEVSVDLDGQTRTAKAAFPVLAHHAIPAVGDPAIP
                     SDNLTVASTGVPPTAIDSRATSAGAIPEPELHRTTIAAALAAHRPIVVVLATPVFCVS
                     RFCGPVTDMVDGLAKRYGDRAAFIHVEIYKDYSKQLINQTAMDWLLRNGDLVEPWVFV
                     IGADGRIVARFDNVTTPGELEPVIQALPAQPG"
     gene            complement(778..1344)
                     /locus_tag="VF954_03970"
     CDS             complement(778..1344)
                     /locus_tag="VF954_03970"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9530272.1"
                     /translation="MSEGPGRGASHTRPDRRRTWLVAAAVVAIAAGGIGAAVALSGSG
                     GSASGGRVGTAAPAFSVDTLRGGGRVSLASAGGRPLVLNFWASWCQPCTQEMPALEAE
                     HRVLGDRVAFVGIDTKDNREDGASFLRKVGVDYVSGFDPLAATFDAYGIIGMPTTFLI
                     RPDGQVLYRHTGGLTQAGLDAVVHRYFG"
     gene            complement(1341..1907)
                     /locus_tag="VF954_03975"
     CDS             complement(1341..1907)
                     /locus_tag="VF954_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530273.1"
                     /translation="MAVIDIAGFVADLKDHAVEHGFHVHDERHFVETYSLRQAWEVDL
                     HPEEGCGGPLDLHLSLEVDPRVLLSFEDLVADLPEDDDPPDEFHFPLTFTWALPPLPA
                     APDLLRLTIDLAGVGGVDLPLEVSAADSFASATDAPERRLTILAHQQVSLARILAGEE
                     LLCETFDRSLAVSRFLLDHADHWLGGAR"
BASE COUNT          302 a          723 c          739 g          257 t
ORIGIN      
        1 gacggtccgg ttccggttcg cgttcctcga cgccgggccg gccggctgcc gtccgacggc
       61 cccggccacg gcggcgttcc tggccatccc gggcacgccc gtgccctcac cgcccccagc
      121 ccgggcccag ctgacctcct cggcgtctgg cgagggcgtg tacggggcgc cggcaggctt
      181 cgaccgggcc ggcaactggg aggtcgaggt gagcgtggac ctcgacggcc agacgcgcac
      241 ggccaaggcg gccttccccg tgctcgccca ccacgccatc ccagcggtgg gtgacccggc
      301 catcccgagc gacaacctga cagtggcgtc cacgggcgtg ccccccaccg ccatcgactc
      361 ccgggccacg agcgccgggg cgatccccga gccggagctg caccgcacga cgatcgccgc
      421 cgccctggcc gcccacaggc cgatcgtggt cgtgctcgcc acgcccgtgt tctgcgtcag
      481 ccggttctgc gggccggtca cggacatggt ggacgggctg gccaagcgct acggcgaccg
      541 ggccgcgttc atccacgtcg agatctacaa ggactacagc aagcagctga tcaaccagac
      601 ggcgatggac tggctgctgc gcaacggcga cctggtggag ccgtgggtct tcgtgatcgg
      661 cgccgacggc aggatcgtgg cccgcttcga caacgtcacc acacccggcg agctcgagcc
      721 ggtgatccag gcgctgccgg ctcagccggg gtagccggct cagccgaagg agcaggctca
      781 gccgaagtag cggtggacca cggcgtcgag gccggcctgg gtcaggcccc ccgtgtgccg
      841 gtacagcacc tggccgtcgg ggcggatcag gaaggtggtc ggcatgccga tgattccgta
      901 cgcgtcgaag gtggcggcga gcgggtcgaa acccgagaca tagtcgactc cgaccttgcg
      961 caggaaggat gcgccgtcct cgcggttgtc cttggtgtcg atcccgacga aggcgacccg
     1021 gtcgcccagc acgcgatgct cggcctccag cgcaggcatc tcctgggtgc agggctggca
     1081 ccacgacgcc cagaagttga gcaccagggg gcgcccgccg gccgaggcga gcgagaccct
     1141 tcctccgcct cggagggtgt cgacagagaa agcgggcgcc gccgtgccca cccgcccgcc
     1201 gctggcgctg ccgcccgagc cggagagcgc caccgcggcc ccgatcccgc cggccgcgat
     1261 ggccacgacg gcggcggcga cgagccaggt gcggcgccgg tccggccggg tgtgcgacgc
     1321 gccccggcca ggcccctcgc tcaccgggcc ccgcccaacc agtggtcggc gtggtcgagc
     1381 aggaacctgc tgacggccag cgagcggtcg aaggtctcgc acagcagctc ctcgccggcc
     1441 agtatccggg ccagagagac ctgctggtgg gcgaggatcg tgagacggcg ctccggcgcg
     1501 tcggtggccg acgcgaacga gtcggcggcc gagacctcga ggggaaggtc gacgccaccg
     1561 accccggcca ggtcgatggt caaccggagc aggtcagggg ccgccggcag ggggggcagg
     1621 gcccaggtga aggtcagggg aaagtggaac tcgtcgggcg ggtcgtcgtc ctcgggcagg
     1681 tcggccacca ggtcctcgaa ggacagcagc acgcgggggt ccacctcgag cgacaggtgc
     1741 aggtcgagcg gcccaccgca accctcctcg gggtgcaggt cgacctccca ggcctggcgc
     1801 agcgagtagg tctcgacgaa gtgccgctcg tcgtggacgt gaaagccgtg ctcgacggca
     1861 tggtccttga gatcggcgac gaacccggcg atgtcgatga cggccatcgc taccgtcctt
     1921 cggtttcctg gacgacgcgt accccctggg cgagcgtgcg gaacgcccgg gcacggtgtg
     1981 agatcgcctg cttctcccgg tcggtcatct ccgcgaaggt a
//
LOCUS       DASWGG010000220         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1827214, whole genome shotgun sequence.
ACCESSION   DASWGG010000220 DASWGG010000000
VERSION     DASWGG010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827214"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VF954_03980"
     CDS             complement(<1..1124)
                     /locus_tag="VF954_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9530274.1"
                     /translation="MTWRPELDELRRREEMARRMGGEANVQRQHDAGRLTVRERVERL
                     LDPGTFDEIGALAGFAEYDAGGSLTSLTPANLVAGTGRIEGRRVVVCGDDFTVRGGAA
                     DASIHEKQVYAERMANELRLPLVRLVDGTGGGGSVKSYEKIGRTYVPANPGWDLVVDN
                     LGKVPVVAACLGPVAGLGAARAVSAHFSVMVRGSSQLFVAGPPVVAAGMRQSVSKEEL
                     GGWEIHTRESGAVDNLAETEDEALAQCRRFLSYLPANAWQLPPVCAPDGDPPGRLVEE
                     LREIVPRSRRLTYDVRRILSLVFDQGTLFEVAPGYGRSCVTALARLAGRPVAVLASDP
                     KVYGGGLTAAASDKMARWVDTADTFHLPVVNFVDQPGFVIG"
     gene            1149..>1562
                     /locus_tag="VF954_03985"
     CDS             1149..>1562
                     /locus_tag="VF954_03985"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX9530275.1"
                     /translation="MKVLVTGGTGLLGSRLVPRMAAAGHQVRVMTHSGRRVVGAPSIT
                     GDLLTGAGVADAVAGAECIVHLASAPQGDTQKVDVLGTRGLVEMARRAGVPHLVFVSI
                     VGVDRVPYAYYKAKLEAEKVVQAGQMPWTILRATQF"
BASE COUNT          225 a          573 c          559 g          205 t
ORIGIN      
        1 ccgatcacga agcccggctg gtcgacgaag ttgaccaccg gcagatggaa ggtgtcggcg
       61 gtatcgaccc aacgggccat cttgtcggag gcggccgcgg tcagcccgcc gccgtagacc
      121 ttgggatccg acgccagcac cgccaccggc cgcccggcca ggcgggcgag ggcggtcacg
      181 caggagcggc cgtaacccgg cgcgacctcg aacagcgtgc cctggtcgaa gaccagcgac
      241 aggatccggc gcacgtcgta ggtcaggcgc cgggagcggg gcacgatctc gcgcagctcc
      301 tccaccagcc gcccgggcgg gtccccgtcc ggcgcgcaga ccggcggcag ctgccaggcg
      361 ttggcgggca ggtaggagag gaaacggcgg cactgggcca gcgcctcgtc ctcggtctcg
      421 gccaggttgt cgaccgcccc tgactcgcgg gtgtggatct cccagccgcc cagttcctcc
      481 ttggagaccg actggcgcat gcccgccgcc accacgggcg ggccggccac aaagagctgg
      541 gaggagcccc gtaccatcac cgagaagtgg gccgagaccg cgcgggcggc acccaggccg
      601 gccaccgggc ccaggcaggc cgccaccacg ggcaccttgc ccaggttgtc caccaccagg
      661 tcccagcccg ggttggcggg cacgtaggtc cgcccgatct tctcgtagga cttgacagag
      721 ccgcccccgc cggtgccgtc gaccaaacgc accaggggca gccggagctc gttcgccatc
      781 cgctcggcat agacctgctt ctcgtggatg ctggcgtcgg cggcgccccc gcggacggtg
      841 aagtcgtcgc cgcagaccac cacccggcgg ccctcgatgc gcccggtgcc ggcgaccagg
      901 ttggccgggg tcagggacgt gagtgaaccg ccggcgtcgt actccgcgaa cccggccagg
      961 gctccgatct cgtcgaaggt gccggggtcg aggagccgct cgacccgctc ccggaccgtc
     1021 agccgcccgg cgtcgtgctg gcgctggacg tttgcctcgc cgcccatccg gcgagccatc
     1081 tcctcccggc gccgcagctc gtcgagctca gggcgccagg tcacgcagga attatggtta
     1141 cccactgtat gaaggtgctg gttaccggcg gcaccgggct gctcgggagc cggctggttc
     1201 cccggatggc ggccgcgggc caccaggtgc gggtcatgac ccattccggg cgtcgggtcg
     1261 tgggagcgcc ctccatcacc ggcgacctgc tcaccggcgc cggggtggcc gacgcggtgg
     1321 cgggcgccga gtgcatcgtc cacctggcca gcgctcccca gggcgatacc cagaaggtgg
     1381 acgtgctggg caccaggggc ctggtcgaga tggcgcgccg ggcaggcgtc ccccacctgg
     1441 tcttcgtctc gatcgtcggc gtcgaccgtg tgccctacgc ctactacaag gccaagctgg
     1501 aggccgagaa ggtggtccag gccggccaga tgccgtggac gatcctgcgc gccacccagt
     1561 tc
//
LOCUS       DASWGG010000221         7694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_855890, whole genome shotgun sequence.
ACCESSION   DASWGG010000221 DASWGG010000000
VERSION     DASWGG010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855890"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2242
                     /locus_tag="VF954_03990"
     CDS             <1..2242
                     /locus_tag="VF954_03990"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX9530276.1"
                     /translation="LSVDDLGAEGIAEVMRVTDAFVEVSLRAIPKVPALRGKTVVSLF
                     YEDSTRTRLSFETAAKRLSADVMSFSVTSSSVKKGESVRDTVETITAMGVDAIVVRHG
                     SAGVPWQVAGWTDAAVINAGDGWHEHPTQALLDCYTIRQARGGLDSEDASAALAGLHV
                     AIVGDVKHSRVARSDVAAFSALGAEVTLVAPPTLLPPSLAGWPVKVSFDLDAVLPTAD
                     VVYLLRIQAERTAEALLPSLREYVAGFGLTGRRARLLRPDALVMHPGPLNRGVEIAAE
                     VADLPRTVITKQVANGVACGWPCSSCSWDRGRALAAEASREVLIRGGTVIDAGGLRPG
                     DVLVHDGMIVAVDEGLAAGRGATVLDAGGALVGPGFVDLHTHLRQPGREEAETVETGS
                     RAAALGGYTAVVAMPNTEPTIDAPGVVREVRELAGGALCEVAVAGAITVGRSGERLAP
                     MAEMAALGVHVFTDDGSGVQDALLMRRAMEYASALGIVLAQHCEDAGLAAGGHMHEGE
                     WSSRLGLAGIPAGAEELMVMRDLALARMTGAPVHFLHLSTAGSVAMVAAAKRGGLTVT
                     AEAAPHHFTLTDASVASYDPVFKVNPPLRSAADVDAVRQGLADGTLDAVATDHAPHPA
                     EAKELPFDQAPPGMLGLETALALGLTHLVADGKGPLSMADLWARMSWGPATIAGLGHH
                     GGGVAPGRPAHLTVVDPSVVWEVRPEALASRSRNTPYAGHKLTGRVRHTVWRGEPVVR
                     DGEAQR"
     gene            2242..3354
                     /gene="carA"
                     /locus_tag="VF954_03995"
     CDS             2242..3354
                     /gene="carA"
                     /locus_tag="VF954_03995"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX9530277.1"
                     /translation="MAGSEAARPAAVPALLVMADGTTFEGEAVGARPVGGVATGELVF
                     NTVMSGYQEVITDPSYAGQVIAFTYPHIGNYGVVPHDDESRRPFCRGVVVRDLARRAS
                     SWRAAEGLDGFLARHGVPAITGVDTRRLTRHVREAGSMPCAFGVADERVLKAAALDEP
                     GTRGVDLVATVTTAVPYRVGKGPRTVVAYDFGIKRTILRHLAGIAEVEVVPAATPAAE
                     VLDRHPDGVFLSNGPGDPAAVAYASDAITGLLGEVPVFGICLGHQLLATALGATTFKL
                     PFGHHGGNHPVRRLAGGAVEITSQNHNYAVDEGSLQGAEVTHVNLNDGVVEGLRARGV
                     PAFGVQYHPEAGPGPHDAEYLFEEFRSLMATMDRRP"
     gene            3358..6666
                     /gene="carB"
                     /locus_tag="VF954_04000"
     CDS             3358..6666
                     /gene="carB"
                     /locus_tag="VF954_04000"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX9530278.1"
                     /translation="MPRRDDLHTILVIGSGPIVIGQACEFDYSGTQACRVLRQEGYRV
                     ILVNSNPATIMTDPDFADRTYVEPLEPDVLAAVIERERPDALLPTLGGQTALNLAMAL
                     WERGVLDAFGVELIGADAEAIATAEDRERFKQAMTEIGLAVPASGFAHTLHEALGIAE
                     AIGYPLIVRPSFILGGAGTGIAHDGEGFGRIAAAGLAASPISEILIERSIAGWKEYEL
                     EVMRDRADNCVVVCSIENFDPMGVHTGDSITVAPAQTLSDVEYQRMRDAAFACIRRIG
                     VDTGGSNIQFAINPDDGDLVVIEMNPRVSRSSALASKATGFPIAKIAARLAVGYTLDE
                     IPNDITRRTPASFEPTIDYVVTKVPRWAFEKFPGTPDILGTRMQSVGEAMAIGRTFPE
                     SLQKGLRSLEHGRWGLNCDPGESQLDAFDDDELVRRASIATPDRPFQLEAALRRGVPL
                     ERLGSATGVDPWFLDQISLIVEERAHLAGLGLGGMERADWRRAKRLGFSDAQLAWLFG
                     AAEAEVRARRLGAGVRATFKTVDTCGAEFEASTPYHYSTYEDTDEVERSAARRVVILG
                     SGPNRIGQGIEFDYCCVHASFALREAGYETVMVNCNPETVSTDYDTSDRLYFEPLTEE
                     DVLNVLEAETAASDPGGEVVGVIVSMGGQTPLKLAGRLPEHLVLGTSPDSIDLAEDRE
                     RWNALCSRLEIPQPAGGTATTVEEALGVVGRIGFPALVRPSYVLGGRAMEITYGEEEL
                     RAAVQQLAGFGSLGREGGLSAGRPVLIDRFLEDAIEVDVDAVRDRTGEVLIGGVMEHV
                     EEAGVHSGDSACVIPPPTLSNQVVGVIGDYTRAIADALDVRGLVNVQYAVKAGQVFVI
                     EANPRASRTVPFVAKATGVPLAKVAARVMLGATLDELRHEGLLRPPADGGHVSVKEAV
                     LPFGRFPDADTILGPEMRSTGEVMGIDATFGLAFAKSQAAAGSRLPEGGTVFLSLADR
                     DKDAGLAAALRFVELGFGIAATRGTARFLEDHGVPVGEIVGKVGHGDGVDAVELIASG
                     KVDLVVNTPRGRGPRADGAHIRRAASVHKVPSLTTVAAARAAAAGIADWAGHPLSVRS
                     LQEYHDRDQLRLPL"
     gene            6677..7624
                     /locus_tag="VF954_04005"
     CDS             6677..7624
                     /locus_tag="VF954_04005"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX9530279.1"
                     /translation="MTRRRGRAPASGVDLTTSIGSLELPNPVMTASGTAGHGAELGAY
                     LDLGSLGAVVVKSLSDEPWPGNPAPRVHPTAGGMLNSVGLQGPGVEVWLEEELPALQA
                     TGARIVASIWARTLEGYYKAASMLAGSPPAVVAVEVNVSCPNIEDRRRMFAHSPSATA
                     EALDAASACGRPRWAKLSPNVTDITEIAAAALGAGAEALTLVNTVMGLAIDVDRRHPV
                     LGGGGGGLSGPAIRPVGVRAVFECHAAFPGAALIGAGGVGSGRDAVEYVMAGARAVQV
                     GTATFADPRAPARVLGEISHWCRRHGVVRLDELIGAAHG"
BASE COUNT         1013 a         2857 c         2794 g         1030 t
ORIGIN      
        1 gctgtcggtg gacgatctcg gcgccgaggg catcgccgag gtcatgcggg tcacggacgc
       61 cttcgtcgag gtcagcctcc gtgccatccc caaggtgccg gcgctgcggg gcaagacggt
      121 ggtgtcgctc ttctacgagg actcgacccg cacccggctg tccttcgaga ccgcggccaa
      181 gcggctgtcg gccgacgtga tgagcttcag cgtcaccagc tcgtcggtga agaaaggcga
      241 gtcggtgcgc gacacggtcg agaccatcac ggccatgggc gtcgacgcca tcgtggtgcg
      301 gcacggctcg gccggcgttc cctggcaggt cgccgggtgg acggacgcgg cggtgatcaa
      361 cgccggcgat ggttggcacg agcaccccac ccaggcgctg ctcgactgct acaccatccg
      421 ccaggcccga ggtggcctcg acagcgagga cgcgtccgcc gcgctggccg ggctgcacgt
      481 cgccatcgtc ggcgacgtca agcacagccg ggtggcccga tcggacgtgg ccgccttctc
      541 cgccctcggt gccgaggtga ccctggtggc gccgcccacg ctgctgccgc cctctctggc
      601 cggctggccc gtgaaggtga gcttcgacct ggacgcggtc ctgcccaccg ccgacgtcgt
      661 ctacctgctg cggatccagg ccgagcgcac ggccgaggcc ctcctgccct ccctgcggga
      721 gtacgtggct ggcttcggcc tcaccgggcg gcgggccagg ctcctgcggc ccgacgccct
      781 ggtgatgcac ccggggccct tgaaccgcgg ggtggagatc gccgccgagg tcgccgacct
      841 cccccgcacg gtgatcacca agcaggtggc caacggcgtg gcgtgcggat ggccgtgctc
      901 ttcctgctcc tgggatcggg gtcgggcctt ggcggctgag gccagtcgtg aggtgctgat
      961 ccggggtggc accgtgatcg acgccggcgg gctccgcccc ggcgacgtgc tggtccacga
     1021 cggcatgatc gtggcggtcg acgaagggct ggccgccgga cggggcgcga ccgtgctcga
     1081 tgccggcggt gcgctcgtag gccccggctt cgtcgacctg cacacccacc tgcgccagcc
     1141 cggccgggag gaggccgaga ccgtcgagac cggctcccgg gccgccgccc tggggggcta
     1201 cacagccgtg gtcgccatgc ccaacaccga gcccaccatc gacgcgcccg gcgtggtgcg
     1261 ggaggtgcgg gagctggcgg gcggcgcgct gtgcgaggtg gcggtggccg gcgccatcac
     1321 cgtcgggcgc agcggcgagc ggctggcgcc gatggccgag atggcggcgc tcggcgtgca
     1381 cgtcttcacg gacgacggct cgggcgtgca ggacgccctc ctcatgcggc gggccatgga
     1441 gtacgcgtcc gcgctcggca tcgtgctggc ccagcactgc gaggacgccg ggctggccgc
     1501 cgggggccac atgcacgagg gcgagtggtc cagccggctg ggcctggcgg ggatcccggc
     1561 gggggccgag gagctgatgg tgatgcgtga cctggcgctg gcccgcatga ccggcgcgcc
     1621 cgtgcacttc ctccatctgt cgaccgcggg gtccgtcgcc atggtcgcgg cggcaaagcg
     1681 cggcgggctg acggtcacgg ccgaggccgc cccccaccac ttcaccctca cggacgcatc
     1741 cgtcgcgtcc tacgacccgg tgttcaaggt caacccgccg cttcgctccg cggccgacgt
     1801 cgacgccgtc cgccagggcc tcgccgacgg gaccctcgac gccgtggcga ccgaccacgc
     1861 gccccacccc gccgaggcca aggagctgcc cttcgaccag gccccgcccg gcatgctggg
     1921 gctggagacg gccctcgccc tgggcctgac gcatctggtc gcggacggta aggggccgct
     1981 cagcatggcc gatctctggg cccgcatgtc ctgggggccg gccaccatcg ccggcctcgg
     2041 ccatcacggc ggcggggtgg cgcccggccg tcccgcccac ctgaccgtcg tcgacccctc
     2101 ggtggtgtgg gaggtccgcc ccgaggccct ggccagccgc agccggaaca cgccgtacgc
     2161 gggccacaag ctgacgggcc gcgtccgcca cacggtgtgg cggggcgagc cggtggtcag
     2221 ggacggggag gcccagcgtt gatggccggg tcggaggcgg ccaggcccgc ggcggtgccc
     2281 gccctgctgg tcatggcgga cgggacgacc ttcgagggcg aggccgtcgg tgcccgtccc
     2341 gtcgggggag tggccaccgg cgagctggtc ttcaacacgg tgatgtccgg ctaccaggag
     2401 gtgatcacgg acccttccta cgccggtcag gtgatcgcct tcacctaccc gcacatcggc
     2461 aactacgggg tcgtgcccca cgacgacgag agccgccggc cgttctgccg gggcgtcgtc
     2521 gtgcgggacc tggcccggcg ggccagcagc tggcgggcgg ccgaggggct ggacggcttc
     2581 ctggcgcgcc acggggtgcc ggccatcacg ggcgtcgaca cccggcgctt gacccgccac
     2641 gtgcgggagg ccggctccat gccgtgcgcc ttcggggtcg cggacgagcg cgtgctcaaa
     2701 gccgccgccc tcgacgagcc gggcacccgc ggtgtcgacc tggtggccac ggtcaccacc
     2761 gccgtcccgt accgggtggg caaagggccg cggaccgtcg tcgcctacga cttcgggatc
     2821 aagcgcacga tcctgcggca tctggccggc atcgccgagg tggaggtcgt acccgcggcc
     2881 acacccgcgg ccgaggtgct ggaccgccac ccggacggcg tcttcctctc caacgggccg
     2941 ggcgacccgg ccgccgtcgc ctacgcgagc gacgccatca ccggcctgct cggcgaggtg
     3001 cccgtcttcg gcatctgcct cggccaccag ctgctcgcca ccgccctcgg ggccacgacc
     3061 ttcaagctcc cgttcggcca ccacggcggg aaccacccgg tccgccgcct ggccggcggg
     3121 gcggtcgaga tcaccagcca gaaccacaac tacgccgtgg acgagggctc cctccagggc
     3181 gccgaggtca cccacgtcaa cctcaacgac ggcgtggtcg aagggttgcg cgcccggggc
     3241 gtgccggcct tcggtgtgca gtaccacccg gaggccgggc ccgggcccca cgacgccgag
     3301 tacctgttcg aggagttcag gtccctcatg gccaccatgg atcggcggcc ctgacgcgtg
     3361 ccccgccgcg acgacctcca caccatcctc gtgatcggct cggggcccat cgtgatcggc
     3421 caggcgtgcg agttcgacta ctccgggacc caggcctgcc gggtgctccg gcaggagggc
     3481 taccgggtca tcctggtcaa ctccaacccg gcgaccatca tgacggaccc cgacttcgcc
     3541 gaccggacct acgtcgagcc cctcgagccg gacgtgctgg ccgccgtcat cgagcgggag
     3601 cgcccggacg cgctgctgcc gaccctcggc gggcagaccg ccctcaacct ggccatggcg
     3661 ctgtgggagc ggggcgtgct ggacgccttc ggcgtcgagc tcatcggcgc cgacgccgag
     3721 gccatcgcca cggccgagga ccgggagcgc ttcaagcagg ccatgaccga gatcggcctc
     3781 gccgtgcctg cttcgggctt cgcccacacc cttcacgagg cgctcggcat cgccgaggcc
     3841 atcggctacc cgctgatcgt gcggccgtcc ttcatcctgg gcggggccgg gaccggcatc
     3901 gcccacgacg gcgaggggtt cgggcgcatc gccgccgccg ggctggcggc cagccccatc
     3961 tcggagatcc tgatcgagcg gtcgatcgcg ggatggaagg agtacgagct cgaggtcatg
     4021 cgcgaccggg ccgacaactg cgtggtggtg tgctcgatcg agaacttcga cccgatgggc
     4081 gtccacaccg gcgactccat caccgtcgcc cccgctcaga cgctctccga cgtcgagtac
     4141 cagcgcatgc gcgacgccgc cttcgcgtgc atccgccgca tcggcgtcga caccggcggg
     4201 tccaacatcc agttcgccat caaccccgac gacggcgacc tggtcgtcat cgagatgaac
     4261 ccacgcgtct cgcggtcctc cgccctcgcc tccaaggcga ccgggttccc gatcgccaag
     4321 atcgccgccc ggctcgccgt cggctacacg ctagacgaga tccccaacga catcacccgc
     4381 aggacgccgg ccagcttcga gcccacgatc gactacgtcg tgaccaaggt gccccggtgg
     4441 gccttcgaga agttccccgg caccccggac atcctgggca cgcggatgca gtccgtcggg
     4501 gaggccatgg ccatcgggcg gacctttccc gagtcgctcc agaagggact gcgctccctc
     4561 gagcacggtc gctggggcct caactgcgat cccggcgaga gccagctcga cgccttcgac
     4621 gacgacgagc tggtccggcg ggcatccatc gccacccccg accgaccctt ccagctggag
     4681 gccgccctgc gccggggcgt cccgctcgag cggctgggtt cggcgaccgg cgtggaccct
     4741 tggttcctcg accagatctc gctgatcgtc gaggagaggg cccacctggc cggcctgggg
     4801 ctgggcggga tggagcgggc cgactggcgc cgggccaagc ggctcggctt ctccgacgcc
     4861 cagctggcct ggttgttcgg cgcggccgag gccgaggtgc gggcccgccg gctgggagcc
     4921 ggtgtgcggg ccaccttcaa gacggtcgac acctgcgggg ccgagttcga ggcgtccacc
     4981 ccgtaccact actcgaccta cgaggacacc gacgaggtcg aacgcagcgc ggcccgccgg
     5041 gtcgtcatcc tcggctcggg ccccaaccgg ataggccagg gcatcgagtt cgactactgc
     5101 tgcgtgcacg ccagcttcgc cctccgcgag gccggctacg agaccgtgat ggtcaactgc
     5161 aacccggaga cggtgtccac cgactacgac acctcggacc ggctctactt cgagcccctg
     5221 accgaggagg acgtcctcaa cgtcctggag gccgagacgg ccgcgtcaga tccgggcggc
     5281 gaggtggtcg gcgtcatcgt gagcatgggc ggccagaccc ccctcaagct ggccggccgg
     5341 ctccccgagc acctggtcct cggcaccagc cccgacagca tcgacctggc cgaggaccgg
     5401 gagcgctgga acgcgctgtg ctcccggctg gagatccccc agccggcggg cggcaccgcg
     5461 accacggtcg aggaggcgct cggggtggtg ggccgcatcg ggttccccgc cctcgtgcga
     5521 ccgagctacg tgctcggcgg gcgggccatg gagatcacct acggcgagga ggagctgcgc
     5581 gcggccgtgc agcagctggc cggcttcggg tccctcggcc gtgagggagg gctgtcggcc
     5641 ggccgcccgg tgttgatcga ccggttcctc gaagacgcca tcgaggtgga cgtggacgct
     5701 gtccgagacc gcaccggcga ggtcctgatc ggcggggtca tggagcacgt cgaggaggcc
     5761 ggggtgcatt ccggcgactc ggcctgtgtc atcccccccc cgacgctgtc caaccaggtg
     5821 gtcggcgtga tcggagacta cacccgggcc atcgccgacg ccctagacgt gcgcggcctg
     5881 gtcaacgtgc agtacgccgt caaggccggc caggtcttcg tgatcgaggc caacccccgg
     5941 gccagccgga ccgtgccgtt cgtggccaag gcgacgggtg tcccgctggc caaagtcgcg
     6001 gcccgggtga tgctcggcgc cacgctcgac gagctccggc acgagggcct cctccgtccc
     6061 ccggccgacg ggggccatgt gagcgtgaag gaggccgtgc tgcccttcgg gcggttcccc
     6121 gacgccgaca ccatcctcgg gcccgagatg cgctcgacag gcgaggtgat gggcatagac
     6181 gccaccttcg gcctggcctt cgccaagagc caggccgccg cgggcagccg gctgccggag
     6241 ggcggcacgg tcttcctgtc gctggccgac cgggacaagg acgccggcct ggcggccgcg
     6301 ctgcggttcg tcgagctggg cttcggcatc gcggccacgc gcggcacggc gcggttcctc
     6361 gaggaccacg gcgttcccgt gggcgagatc gtcggcaagg tgggccacgg cgacggtgtc
     6421 gacgccgtgg agctcatcgc cagcggcaag gtcgacctgg tggtcaacac gccgcgcggc
     6481 cggggccccc gtgccgacgg ggcccacatc cgacgggcgg ccagcgtgca caaggtgccg
     6541 agcctcacga ccgtggccgc ggcgcgcgcc gccgccgccg gcatcgccga ctgggccggt
     6601 cacccgctgt cggtgcgcag cctccaggag taccacgacc gggaccagct ccggctcccc
     6661 ctatgacccg gcccctatga cccggcgccg gggccgcgca ccggcatccg gggtcgatct
     6721 gaccacgtcg atcggatcgc tggagctgcc gaaccccgtc atgacggcat ccgggacggc
     6781 gggccacggc gccgagctgg gcgcctacct ggacctgggc tcgctcggcg ccgtggtcgt
     6841 gaagtccctg tccgacgagc cctggcccgg caaccccgcg ccccgggtgc acccgacggc
     6901 cggcggcatg ctcaacagcg tcgggctcca gggccccggt gtcgaggtct ggctcgagga
     6961 ggagctgccc gcgttgcagg ccaccggcgc ccgcatcgtg gcgagcatct gggcccgcac
     7021 gctcgagggc tactacaagg cggcgtccat gctggccggc tcgcctcccg ccgtcgtggc
     7081 cgtggaggtc aacgtaagct gccccaacat cgaggaccgg cggcggatgt tcgcccactc
     7141 gccgtcggcg acggccgagg ccctcgacgc ggcctcggcg tgcgggcgcc cgaggtgggc
     7201 caagctgtct cccaacgtga ccgacataac cgagatagcg gcggcggccc tgggggccgg
     7261 ggccgaggcc ctcacgctgg tcaacacggt gatgggcctg gccatcgacg tcgatcgccg
     7321 gcacccggtg ctgggcggcg gtggcggcgg gctgtcgggc ccggccatcc ggcccgtcgg
     7381 ggtgcgcgcc gtcttcgagt gccacgccgc cttcccgggc gccgccctga tcggcgcggg
     7441 cggcgtcggg tcggggcgcg acgcggtcga gtacgtgatg gcaggcgccc gggccgtgca
     7501 ggtgggcacg gcgacgtttg ccgatccccg cgccccggcc cgggtcctgg gcgagatctc
     7561 ccactggtgc cggcgccacg gggtggtccg gctcgacgag ctgatcggag cagcccatgg
     7621 ctgagaccgc ccccgccacc gagctgcccg cagaggtccg tgaccgcctc gccgtggcgc
     7681 tggacgtcga cgac
//
LOCUS       DASWGG010000222         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3113380, whole genome shotgun sequence.
ACCESSION   DASWGG010000222 DASWGG010000000
VERSION     DASWGG010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3113380"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VF954_04010"
     CDS             complement(<1..246)
                     /locus_tag="VF954_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530280.1"
                     /translation="MTPSPNAVRRASTETMRRRPARWLVPLLLAASLVSCTHAKTKTS
                     SADCFPSAGGGGPESVIASVASYDVAVGPPSRLIVGLQ"
     gene            complement(249..680)
                     /locus_tag="VF954_04015"
     CDS             complement(249..680)
                     /locus_tag="VF954_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530281.1"
                     /translation="MTASPRACGGWAAVALLSLLAQSGCHRATRRPPDIPGAGVPGAD
                     LAAAVASLCQARDQAGHETGAARDTFYVVAHQRLHLEAQALAEVDRPAAGRLLIDMQR
                     VEADLAPQAAAPALAADLATLATTAAAGLTRLAVPAPRCAS"
     gene            complement(677..1357)
                     /gene="phoU"
                     /locus_tag="VF954_04020"
     CDS             complement(677..1357)
                     /gene="phoU"
                     /locus_tag="VF954_04020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX9530282.1"
                     /translation="MTETRRSFHEELEQLRHDVIVLAAAAEEAIQAGTHALLDRDLTA
                     VERVIADDRTIDDLTHSAEERCYELLARQQPMAVDLRTLVTVMRILHELERIGDLMVN
                     VAKTTRRLFPGELEPRVRGIIDRMRDQASVQVRLAVDAFADRDPARAAALVDMDDVMD
                     DLQKELFRTIFAGPADDDHALQRAVQVALVGRYFERIADHAVNVAERVEFMVTGQFGS
                     PDRTARRA"
     gene            complement(1354..2079)
                     /locus_tag="VF954_04025"
     CDS             complement(1354..2079)
                     /locus_tag="VF954_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9530283.1"
                     /translation="MIEPQPTTVLVVEDEESFVDALTVGLSREGFSVRVARDGSEALA
                     MFDAVQPDLVLLDIMLPKVSGVDVCRDLRARSNVPIIMVTAKGEEIDAVVGLEVGADD
                     YVTKPYRMRELIARVRAVLRRSPTARPVPVGDEEDLLPIEVGDVSLDPDRHEVRVRGE
                     EIKLPLKEFELLELLLANAGRVLTRETLIDRIWGPDYVGDTKTLDVHVKRLRSRVEHD
                     PSNPTRITTIRGVGYRYEAGARR"
     gene            complement(2076..>3163)
                     /locus_tag="VF954_04030"
     CDS             complement(2076..>3163)
                     /locus_tag="VF954_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9530284.1"
                     /translation="VDQLRDRLRQTEHGLSQARREAERFRRALDAGDRAVVIADREGA
                     VVYRNLTANALVDARHGDALAARVVEEALAEARASGHGAVRTLELHGPPPRTLVVTTS
                     SLGAGGEALGTVAMIEDVTQRKQLEAVRRDFVANVSHELRTPVGAVGLLAETMVGETD
                     PAVLRRLSQRITVESERVAQIINELLDLSRMEAGGLEALEPVAIGPVITAAVDRVRPV
                     AEVAGVTIEGPGADDGAQVMGHRAQLVSAISNLVDNAVKYSDAGTTVSVTVTVGDWID
                     VAVGDTGIGIPARELDRIFERFYRVDRARSRATGGTGLGLSIVRHTAQNHGGEVLVTS
                     REGEGSVFTLRLPRSGPAGSGPAEAGA"
BASE COUNT          427 a         1132 c         1167 g          437 t
ORIGIN      
        1 ctggagaccg acgatgagcc gggagggcgg tcccaccgcg acgtcgtagg acgccaccga
       61 ggcgatcacc gattccggcc cgcccccgcc ggcggaggga aagcaatccg cgctggaggt
      121 cttggtcttc gcgtgggtgc aagacaccag cgaggccgcc agcagcaggg gtacgagcca
      181 tcgggcgggg cggcggcgca tggtctccgt gctggctcgg cggacggcgt tcggtgaggg
      241 tgtcatgatc atgaggcgca gcggggcgca gggacggcga ggcgggtgag cccggctgcc
      301 gccgttgtcg ccaacgtagc cagatccgcg gccagggcgg gcgcggccgc ctgcggcgcc
      361 aggtcggcct ccacgcgctg catgtcgatc agcagccggc ccgccgccgg ccggtcgacc
      421 tcggccaggg cctgggcctc gaggtgcagg cgctggtggg ccacgacgta gaaggtgtcg
      481 cgcgccgcgc cggtctcgtg gccggcctgg tcgcgggcct ggcagagcga cgccaccgcg
      541 gccgccaggt cggcgccggg aaccccggca ccggggatgt cgggcggcct cctggtcgcc
      601 cggtggcacc ccgactgggc gagcagcgac agcagcgcca ccgcggccca acccccgcag
      661 gcgcgaggcg acgcggtcac gcccggcgag ccgtgcggtc cggcgacccg aactgccccg
      721 tgaccatgaa ctcgacccgc tcggcgacat tgacggcgtg gtcggcgatc cgttcgaagt
      781 accgcccgac cagggcgacc tgcacggccc gttgcagggc gtggtcgtcg tcggccggcc
      841 cggcgaagat ggtcctgaac agctccttct ggaggtcgtc catgacgtcg tccatgtcga
      901 ccagggcggc cgctcgagcc gggtcgcggt cggcgaaggc gtccaccgcg agccggacct
      961 ggaccgaggc ctggtctcgc atccggtcga tgatgccccg gacgcggggc tcgagctcgc
     1021 cggggaagag ccgacgcgtg gtcttggcca cgttgaccat caggtcgccg atgcgctcca
     1081 gctcgtggag gatccgcatc accgtcacca gcgtccgcag gtccacggcc atgggctgct
     1141 gccgggccag cagctcgtag cagcgctcct cggccgaatg ggtcaggtcg tcgattgtgc
     1201 ggtcgtcggc gatgacccgc tccacggcgg tgaggtcgcg atcgagcagg gcgtgggtgc
     1261 cggcctggat ggcctcctcg gcggcggcgg ccagcacgat cacgtcgtgg cggagctgct
     1321 cgagctcctc gtggaagctg cgccgggtct cggtcatcgc cgggcgccgg cctcgtagcg
     1381 atagcccacc ccccggatgg tagtgatgcg cgtcgggttc gacggatcgt gctcgacccg
     1441 ggaccggagc cgcttgacgt gcacgtccag ggtcttggtg tccccgacgt agtcggggcc
     1501 ccagatccgg tcgatgagcg tctcccgcgt caggacccgc ccggcgttgg ccagcagcag
     1561 ctcgagcagc tcgaactcct tgagcgggag cttgatctcc tccccgcgga cccgcacctc
     1621 atggcgatcc gggtcgaggc tgacgtctcc cacctcgatg gggagcaggt cctcctcgtc
     1681 gcccacgggg accgggcggg cggtcggcga acgccggagg acggcgcgga cgcgggcgat
     1741 cagctcccgc atccggtacg gcttggtgac gtagtcgtcg gcaccgacct ccagcccgac
     1801 gacggcgtcg atctcctcgc ccttggccgt gaccatgatg atgggcacgt tggagcgggc
     1861 ccgcaggtcg cggcagacgt ccactcccga caccttgggc agcatgatgt cgagcaggac
     1921 gagatcgggc tggacggcgt cgaacatggc gagggcctcg gacccgtccc gcgccacccg
     1981 gacagagaac ccctcccggc tcaggccgac ggtgagggcg tcgacgaagg actcctcgtc
     2041 ctcgacgacg agcaccgtgg tgggctgcgg ctcgatcatg ccccggcctc ggcaggccca
     2101 gacccggcag gcccagatct cggcaggcgc aatgtgaaca ccgacccctc cccttcgcgc
     2161 gacgtgacga ggacctcccc gccgtggttc tgcgccgtgt ggcgcacgat ggacagcccc
     2221 agccccgtgc cgcccgtggc ccggctgcgg gcccggtcca cgcggtagaa ccgctcgaag
     2281 atgcggtcga gctcgcgggc ggggatgccg atgcccgtgt cgcccacggc cacgtcgatc
     2341 cagtcgccga ccgtcaccgt cacgctcacc gtcgtgccgg cgtcggagta cttgaccgcg
     2401 ttgtcgacga ggttggagat cgccgagacc aactgggccc ggtggcccat tacctgggct
     2461 ccgtcgtcgg ctccgggccc ttcgatggtg acaccggcga cctcggccac ggggcggacc
     2521 cgatcgaccg cggcggtgat gaccggcccg atggcgaccg gctctagcgc ctcgaggcca
     2581 ccggcctcca tccgcgacag gtcgagcagc tcgttgatga tctgcgcgac ccgctcggac
     2641 tcgacggtga tgcgctggga gagccggcgc agcacggcgg gatcggtctc ccccaccatc
     2701 gtctcggcca gcaggccgac tgcgccgacc ggggtgcgca gctcgtggct gacgttggcc
     2761 acgaagtcgc ggcggacggc ctcgagctgc ttgcgctggg tcacgtcctc gatcatcgcg
     2821 accgtgccca gggcttcacc gcccgcgccc agtgaagagg tcgtgaccac gagcgtccgc
     2881 ggcggagggc cgtgcagctc gagcgtccgg acggcgccgt gccccgacgc tcgagcctcg
     2941 gccagggcct cttcgacgac gcgggcggcg agcgcatccc cgtggcgggc gtcgaccagg
     3001 gcgttggcgg tcaggttccg gtacacgaca gcgccctcac ggtcggcgat gaccacggcg
     3061 cggtcgccgg cgtccagggc ccgccggaac cgttcggctt cccgccgggc ctgggacagg
     3121 ccgtgctcgg tctggcgcag ccggtcgcgc agctggtcca cgt
//
LOCUS       DASWGG010000223         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3113412, whole genome shotgun sequence.
ACCESSION   DASWGG010000223 DASWGG010000000
VERSION     DASWGG010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3113412"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VF954_04035"
     CDS             complement(<1..642)
                     /locus_tag="VF954_04035"
                     /inference="COORDINATES: protein motif:HMM:NF020028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase"
                     /protein_id="HEX9530285.1"
                     /translation="MDVTDVSWDLSDLLGGRGEEAVDELLDDADARAAALATRRGQVA
                     AFAVEDLRGFMAERAELEEVLGRAQAYALLRFATDTVDPARGALLQRVQERTAAIATR
                     LVFFDVEWAGLDDQRSAELLAGDGLDLCRHHLQTVRRYRGHLLTEPEEQVMTEKAVTG
                     RGAWNRLFNQLTSTIEVSLDGATLSLEEGLSRLQHPDREVRRAAAEAVTAGLAS"
     gene            complement(648..>2093)
                     /locus_tag="VF954_04040"
     CDS             complement(648..>2093)
                     /locus_tag="VF954_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530286.1"
                     /translation="MARWRRQVPQIESDWPPLRPILVRSAEDLGALRLFDPDYPERVM
                     VAAGAPWFMTLFGRDSILTSWMALPVDPDLALGVLQTLARFQGTTVDPRHDEEPGRIL
                     HEIRFGEAASLSLGGGRVYYGSADATPLFVMLLGELRRWGLAREIVDQLLPHADRALQ
                     WIEEYGDRDGDGYVEYQRATDAGLQNQGWKDSWDGVRFATGEVARAPIALCEVQAYVY
                     GAYLARAHFAWERGETAIEQRYQARAASLKAAFNRDFWLPDRGWYAMGLDHEKRPIDA
                     LASNMGHCLWTGIVDEDKAPLVASRLLSEEMFSGWGIRTLASTMVGYNPISYHCGSVW
                     PHDTAIVAAGLMRYGFVAEAQRVILALFDAARLQGGRLPELFCGLRRDEFPSVVGYPT
                     SCSPQAWAAASPMLALRTLLRVEPWVPRGKVWIAPVLPDRIKHLRVDRIPLAGGRVSI
                     EVDRGDVKVEGLPPGLELVEEARRPLTAEHD"
BASE COUNT          304 a          780 c          739 g          270 t
ORIGIN      
        1 cgaggccagg ccggccgtca ccgcctccgc ggccgcccgc cgcacctccc gatcggggtg
       61 ctggagccgc gacagccctt cctccagcga caaggtggcg ccgtccaggc tgacctcgat
      121 cgtcgaggtg agctggttga acaggcggtt ccaggccccg cggccggtga ccgccttctc
      181 agtcatgacc tgctcctccg gctcggtcag cagatggccg cggtaacggc ggacggtctg
      241 gagatggtgc cggcagagat cgaggccgtc cccggccagc agctcagcag accgctggtc
      301 gtcgagaccc gcccactcga cgtcgaagaa gaccagccgg gtggcgatcg cggccgtgcg
      361 ctcctggacc cgctggagca gcgcgcccct ggccgggtcg accgtgtcgg tggcgaaacg
      421 cagcaaggcg taggcctggg cccggccgag cacctcctca agctcggccc tctcggccat
      481 gaagccgcgc aggtcctcga cggcgaacgc cgccacctgg ccccggcggg tggccagggc
      541 ggcggcccgg gcgtcggcgt cgtccagcag ctcatcgacc gcctcctcgc cgcgaccgcc
      601 caggagatcg gagaggtccc acgagacgtc tgtcacgtcc atgtgcgtca gtcgtgctcg
      661 gcggtcagcg ggcgccgggc ttcctcgacc agctcgagcc caggaggcag gccctcgacc
      721 ttgacgtcgc cccggtcgac ctcgatcgac acccgccccc cggccagggg gatgcggtcc
      781 accctcaggt gcttgatccg gtcgggcagc acgggcgcga tccacacctt gccccggggc
      841 acccacggct cgacgcgcag cagcgtccgc agggcgagca tcggggaggc cgccgcccat
      901 gcctgggggg agcaggaggt cggatagccc accaccgacg ggaactcgtc gcggcgcagg
      961 ccgcagaaca gctcgggcag ccggcccccc tgcaaccggg ccgcgtcgaa cagcgccagg
     1021 atgacccgct gggcctcggc cacgaacccg taccgcatga gccccgccgc cactatggcc
     1081 gtgtcgtggg gccacaccga cccgcagtgg tagctgatcg ggttgtagcc gaccatcgtg
     1141 gaggccaggg tgcggatgcc ccagccggag aacatctcct ccgagagcag ccgcgaggcc
     1201 accaacgggg ccttgtcctc gtccacgatg cccgtccaca ggcagtggcc catgttggac
     1261 gccagggcgt cgatcggccg cttctcgtgg tccagcccca tggcgtacca gccgcggtcg
     1321 ggcagccaga agtcacggtt gaacgccgcc ttcagggagg cggcccgggc ctggtaccgc
     1381 tgctcgatgg cggtctcgcc ccgctcccag gcgaagtggg cccgggccag gtacgcgccg
     1441 tagacgtagg cctgcacctc gcagagggcg atgggggccc tggccacctc tccggtggcg
     1501 aagcggactc cgtcccagga gtccttccag ccctggttct ggagcccggc gtcggtggcc
     1561 cgctggtact ccacgtaacc gtcgccgtcg cggtcgccgt actcctcgat ccattggagg
     1621 gcccggtcgg cgtggggcag cagctggtcg acgatctcgc gggccaggcc ccagcggcgc
     1681 agctcgccca gcagcatgac gaacagcggg gtggcatcgg ccgacccgta gtagacccgg
     1741 ccgccgccga gcgacaggct ggcggcctca ccgaagcgga tctcgtgcag gatccggccg
     1801 ggctcctcgt cgtggcgggg gtcgaccgtc gtgccctgga agcgggcgag ggtctgcagc
     1861 acccccaggg ccaggtccgg gtctaccggc agcgccatcc acgacgtcag gatggagtcc
     1921 cgcccgaaca gcgtcatgaa ccacggcgcc ccggccgcca ccatcacccg ctccgggtag
     1981 tcggggtcga acagccgcag ggcgcccagg tcctcggcgc tgcgcaccag gatgggccgc
     2041 agcggcggcc agtcgctctc gatctggggg acctgtcggc gccaacgggc cat
//
LOCUS       DASWGG010000224         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1185302, whole genome shotgun sequence.
ACCESSION   DASWGG010000224 DASWGG010000000
VERSION     DASWGG010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185302"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VF954_04045"
     CDS             <1..494
                     /locus_tag="VF954_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9530287.1"
                     /translation="PLVAAVTTRRLRYMGKDSMWKVHWVGAVFTALGAFPVRRGRADR
                     EALRRCVEVLEGGEALVLFPEGSRRSGPTVEEVFEGAAYLAARTGAPIVPVGIGGSEA
                     AMPRGARFIRPVRTRVVVGAPLRPPAGPEGSRVARRAVRELTAELQGALQRLLDEAAV
                     SPR"
     gene            complement(481..1527)
                     /gene="ispH"
                     /locus_tag="VF954_04050"
     CDS             complement(481..1527)
                     /gene="ispH"
                     /locus_tag="VF954_04050"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX9530288.1"
                     /translation="MEVERVLLAEPRGFCAGVEMAIKALAWMVRVFEPPVYCYHEIVH
                     NQIVVDRFRRLGVVFVDDVADVPDGAPLMLSAHGSAPDVVAAARARGGAVVDAVCPLV
                     TKVHHELGVRAKKGYTVLYVGHEGHDEAVGTLAVAPTACRLVETEHDVRALPDPGTPV
                     ALLAQTTLSHDEWAGIKDQALARFPDLWMAGRSDLCFATTNRQAALKAVAGDADAVVV
                     IGSANSSNTIALEKVARASGAARVHRVNSAAELPEDLSGTVAVTAGASAPEELVRAVL
                     DRLAPRQGVHPVRVTDEDEYFPPPPELRDLLRGLSALVSAAVGAPPRTTLPLADDRQV
                     AASDVLDALGANAG"
     gene            1568..>2599
                     /locus_tag="VF954_04055"
     CDS             1568..>2599
                     /locus_tag="VF954_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEX9530289.1"
                     /translation="MSLPRVVAVDAGSPAARAGLVPGDEIVAMNGRSPRDVIQYRLLA
                     DEAELELDVRRGGLDVTIGITKDAGAPLGVEVQSALFDQVRTCDNHCEFCFIYQLPPG
                     MRSSLYLKDDDYRLSFLYGNFTTLTRFTEADLERVITEHLSPLHVSIHATDPAVRSRM
                     LRNRRGATSLRWLRALLDAGIEVRGQVVVCPGVNDGDVLTDTLTGVLDTYPELSTLAI
                     VPLGVSKFSTESALRAHTPAEAGAVVETVEEWQRIYLRTLGRRLAFCADEYYLLAQRP
                     FPPTPAYEGCPMHEDGVGMARTFEQEFLGRAEVAVRPKHGFFQWADTASRPDGYRFAR
                     TSARTTTKES"
BASE COUNT          346 a          927 c          959 g          367 t
ORIGIN      
        1 cccccctcgt ggcggcggtg accacccgcc ggctgcgcta catgggcaag gacagcatgt
       61 ggaaggtcca ctgggtcggc gccgtgttca cggcgctggg cgccttcccg gtgcgccgcg
      121 gcagggccga tcgcgaggcc ctgcggcgtt gcgtcgaggt gctcgagggg ggagaggcgc
      181 tggtgctgtt cccggaaggc tcccggcgca gcgggcccac cgtcgaggag gtcttcgagg
      241 gggccgccta cctggccgcc cggaccggcg cccccatcgt cccggtcggc atcggtggct
      301 cggaggcggc catgccgagg ggggccaggt tcatccggcc ggtgcggacg cgggtcgtgg
      361 tgggcgcgcc gctgcgcccc ccggcggggc ccgagggcag tcgggtcgcc cggcgggccg
      421 tgcgggagct caccgccgag ctgcaagggg cgctccagcg cctcctcgac gaggcggcgg
      481 tcagcccgcg ttagcgccga gcgcgtcgag cacgtcgctg gccgccacct gccggtcgtc
      541 ggccagggga agcgtcgtcc ggggcggggc gccgaccgcc gccgagacga gggccgagag
      601 gccgcgcagc aggtcgcgca gctcgggcgg cggggggaag tactcgtcct cgtcggtcac
      661 gcgcacggga tgcacgccct ggcggggggc gagacggtcg agaaccgccc gcaccagctc
      721 ctccggcgcc gaggcgccgg ccgtcacggc cacggtcccc gacaggtcct cgggcagctc
      781 ggcggcgctg ttgacccggt ggacgcgggc tgcgcccgac gctcgggcca ccttctcgag
      841 ggcgatggtg ttggacgagt tggccgagcc gatgaccacc acggcgtcgg cgtcgccggc
      901 gacggccttg agggcggcct gccggttggt ggtggcgaag cacaggtcgc tgcgccccgc
      961 catccacagg tcggggaagc gggctagggc ctggtccttg atgccggccc actcgtcgtg
     1021 ggacagggtc gtctgggcca ggagggccac gggcgtgccg ggatcgggca gggcgcgcac
     1081 gtcgtgctcc gtctcgacca ggcggcaagc cgtcggagcg accgcgaggg tgccgaccgc
     1141 ctcgtcgtgg ccctcgtggc cgacgtagag cacggtgtag cccttcttgg cccggacccc
     1201 gagctcgtgg tggaccttgg tcacgagggg gcacacggcg tcgacgaccg cgcctcccct
     1261 ggcgcgggct gcggccacca cgtccggggc cgagccgtgc gccgacagca tcaggggggc
     1321 gccgtcgggc acgtcggcga catcgtccac gaagaccacc ccgagccgcc ggaaccggtc
     1381 gaccacgatc tggttgtgca cgatctcgtg atagcagtac acgggcggct cgaagacccg
     1441 caccatccac gccagggcct tgatcgccat ctccacgccg gcgcagaagc cgcggggttc
     1501 ggccagcagc acccgctcca cttccacggg cccaggctag cggcgggcca gcacctatcc
     1561 tgtcgccatg tcactgcctc gggtggtggc cgtggacgca ggttcgccgg ccgcccgggc
     1621 aggcctggtt ccgggcgatg agatcgtcgc catgaacggc cgatcccccc gcgatgtcat
     1681 ccagtaccgc ctgctcgccg acgaggccga gctcgagctc gacgtccgcc ggggcgggct
     1741 cgacgtcacc atcggcatca ccaaggacgc gggcgccccg ctgggggtcg aggtgcagag
     1801 cgcgctgttc gaccaggtca ggacgtgcga caaccactgc gagttctgtt tcatctacca
     1861 gctcccgccg ggcatgcgat cgagcctcta cctgaaggac gacgactacc ggctgtcgtt
     1921 cctctacggc aacttcacga cgctgacccg attcaccgag gccgatctcg agcgggtgat
     1981 caccgagcat ttgtcgccgc tgcacgtgtc gatccacgcc accgatcctg cggtgcggtc
     2041 gcggatgttg cgcaatcgcc gcggcgccac gagcctccgg tggctgcgcg cccttctcga
     2101 cgcgggcata gaggttcgag gccaagtcgt cgtgtgcccg ggcgtcaacg acggcgacgt
     2161 gctcaccgac acgctcaccg gcgtgctcga cacctacccg gagctttcca cgctggcgat
     2221 cgtgccgctc ggggtttcga agttctcgac ggagagcgcg ctgcgcgccc acacccccgc
     2281 cgaggcgggg gccgtcgtcg agacggtcga ggagtggcag cgcatttatc tgcgcacgct
     2341 tgggcggcgc ctggcgttct gtgccgacga gtactacctg ctcgcccagc ggcccttccc
     2401 gcccactccc gcctacgagg gatgtccgat gcatgaggat ggcgtcggga tggcgcgcac
     2461 gttcgaacag gaattcctcg ggcgcgccga ggtggccgtg cggcccaagc acggcttttt
     2521 ccagtgggcc gacacggcgt cgcgccccga cggctaccgg ttcgcccgca cgtcggctcg
     2581 aaccaccacg aaggagtct
//
LOCUS       DASWGG010000225         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3114430, whole genome shotgun sequence.
ACCESSION   DASWGG010000225 DASWGG010000000
VERSION     DASWGG010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3114430"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VF954_04060"
     CDS             complement(<1..301)
                     /locus_tag="VF954_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX9530290.1"
                     /translation="MPYEVSTAVFEGPFDLLLHLITREEVDLYEVSLSRIVDAYVAEI
                     ARMDRLDLDVATEFVLIAATLIELKVRRLLPGRDDVELDDELALWEERDLLLARLL"
     gene            417..1403
                     /gene="trpS"
                     /locus_tag="VF954_04065"
     CDS             417..1403
                     /gene="trpS"
                     /locus_tag="VF954_04065"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX9530291.1"
                     /translation="MARVFSGVQPSGDMHIGNYLGAFRPWVAHQSEHDAIYCVVDLHA
                     LTLEHDPAVLRERTVETVRVLIAVGLDPDVCTIFVQSHVAEHTSLTWLLECTATFGEL
                     RRMTQFKDKSGGQESVRAGLLTYPVLMAADILLYDTDRVPVGDDQRQHLELARDVATR
                     FNHHYGDTFVVPEAAIPPVGARVMDLQEPTRKMSKSVDSPQGTVGVLDGPDLITRKIK
                     RAVTDPGSDVRFDPERQPGVANLLQLLSAATGRSIDSLAGASYRGYGDLKAQVAEALV
                     ETLRPVQQRYAELADDPGAVSDVLTLGAARAEKIAAATLGRACDAIGLLPRP"
     gene            complement(1446..2168)
                     /locus_tag="VF954_04070"
     CDS             complement(1446..2168)
                     /locus_tag="VF954_04070"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9530292.1"
                     /translation="MATNKRRQQNVVLALLGLVLVYLVARRHLISADKVILVAVLVPS
                     VILHEVSHGAVAYLFGDDTAKRAGRLTLNPLPHIDPFGSIILPALLAFSGAPPFGYAK
                     PVPVAVNRLRSPRNQSLLVSLVGPGVNTVLSVSAALAMRMWFSRQAIDRALQFQGPML
                     PRLLVAFGFINAILAAFNLIPIPPLDGSAVIERLLPRSAWPGYLRLRQYSMPVLFVVV
                     LLRPELLDRVFRPALDLWARLL"
     gene            complement(2191..2538)
                     /locus_tag="VF954_04075"
     CDS             complement(2191..2538)
                     /locus_tag="VF954_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX9530293.1"
                     /translation="MSNDAVIAAQRTQLEQERAELRRQLAELGFGDDGGSGLNYDPNF
                     ADSSQVTAERGEAEALANSLREALDEVERALQKVTDGTYGTCESCGDQIAEPRLEAMP
                     AARYCITCASAKR"
     gene            complement(2550..>2734)
                     /locus_tag="VF954_04080"
     CDS             complement(2550..>2734)
                     /locus_tag="VF954_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9530294.1"
                     /translation="VLRHSCATHMLDHGADIRVVQELLGHASIATTQVYTRVSAERLR
                     VVYEQAHPRARGRSGG"
BASE COUNT          429 a          956 c          990 g          359 t
ORIGIN      
        1 cgagcagccg ggccaggagc aggtcccgct cctcccacag ggcgagctcg tcgtccagct
       61 cgacgtcgtc gcgccccggc agcagccggc ggaccttgag ctcgatgagc gtcgccgcga
      121 tgagcacgaa ctcggtggcc acgtccagat cgagccggtc catgcgggcg atctcggcca
      181 cgtaggcgtc gacgatccgg ctgagcgaca cctcgtagag gtcgacctcc tcacgggtga
      241 tgaggtgaag aagaaggtcg aagggcccct cgaagacggc cgtcgacacc tcgtagggca
      301 tcgcgacgag gatagcggcg gcccttcccc ggagcgcgca tcccgcacag ccggagcgcg
      361 catcccgtac agaatgccgg ggagcccgcc gggaccgcgg cgtaccatga ccggccatgg
      421 cacgggtctt ctcgggcgta cagccctcgg gcgacatgca catcgggaac tacctcggcg
      481 ccttccgccc ctgggtggcc caccagagcg agcacgacgc catctactgc gtggtcgacc
      541 tgcacgcgct gaccctcgag cacgatcccg ccgtgctccg ggagcgcacc gtcgagacgg
      601 tccgggtcct gatcgccgtg ggcctcgacc ccgacgtgtg caccatcttc gtgcagagcc
      661 acgtggccga gcacacgtcg ctcacctggc tgctggagtg cacagccacc ttcggcgagc
      721 tgcgccgcat gacccagttc aaggacaagt cgggcggcca ggagtcggtc cgggccgggc
      781 tgcttaccta cccggtgctc atggctgccg acatccttct gtacgacacc gaccgggtgc
      841 cggtgggcga cgaccagcgc cagcacctgg agctggcccg ggacgtggcc acgcggttca
      901 accaccacta cggggacacg ttcgtggtgc cggaggcggc catcccgccg gtcggggcga
      961 gggtcatgga cctccaggag ccgacccgga agatgtccaa gtcggtcgac tccccccagg
     1021 gcaccgtcgg ggtgctcgac ggccccgatc tcatcacccg gaagatcaag cgggccgtga
     1081 ccgatcccgg ctcggacgtg cgcttcgacc cggagcgcca gccgggcgtg gccaacctgc
     1141 tccagctgct ctcggcggcg accggccgca gcatcgactc cctggccggt gcgagctacc
     1201 ggggctacgg cgacctcaag gcccaggtgg ccgaggcgct ggtcgagacc ctgcgacccg
     1261 ttcagcaacg ctacgccgag ctggccgacg atccgggggc cgtctccgac gtgttgacgc
     1321 tgggagcggc ccgggcggag aagatcgccg ccgccaccct cggccgggcc tgtgatgcca
     1381 tcgggctgct gccccgcccc tgaacccagg ccgcgacgcc cgaggaaccg cctggccatg
     1441 gagctctaca ggaggcgggc ccacaggtcc agggcgggcc ggaacacccg gtcgagcaac
     1501 tcgggccgga gcagcaccac cacgaacagc accggcatcg agtactgccg gagccggagg
     1561 tagcccggcc aggccgagcg gggcaggagg cgctcgatga ccgccgagcc gtctagcggc
     1621 gggatgggga tgaggttgaa ggcggccagg atggcgttga tgaagccgaa ggcgacgagc
     1681 agcctcggaa gcatcgggcc ctggaactgg agggcgcggt cgatggcctg cctcgagaac
     1741 cacatgcgca tcgccagcgc ggccgagacc gacagcacgg tgttgacgcc ggggccgacc
     1801 aggctgacca gcaggctctg gttgcgcggg ctgcgcagcc ggttgacggc gaccgggacg
     1861 ggcttggcgt agccgaacgg cggggcgccg ctgaaggcca gcagggcggg caggatgatc
     1921 gagccgaagg ggtcgatgtg cgggagcggg ttcagcgtga ggcgcccggc ccgcttggcg
     1981 gtgtcgtcgc cgaagaggta ggcgaccgcg ccgtgggaca cctcgtgcag gatgaccgac
     2041 ggcaccagca cggcgaccag gatgaccttg tcggccgaga tgaggtgccg gcgggcgacc
     2101 aggtacacca gcacgaggcc cagcagggcc agcaccacgt tctgctgccg gcgcttgttg
     2161 gtggccacta ccagccccgg gcggggtggc tcaacgcttg gccgaggcgc aggtgatgca
     2221 gtagcgggcc gccggcatgg cttccagccg gggctcggcg atctggtccc cgcacgactc
     2281 gcaggtcccg tacgtgccgt ccgtcacctt ctgaagggcg cgctccacct cgtcgagcgc
     2341 ctcccggagg ctgttggcca gggcctcggc ctcaccgcgc tcggccgtga cctggctcga
     2401 gtccgcgaag ttggggtcgt agttgagccc gctgcccccg tcgtcgccaa aacccagctc
     2461 ggccagctgg cggcgcagct cggcgcgctc ctgctccaac tgcgtgcgct gggccgcgat
     2521 gacggcgtcg ttcgacatgg ccaaccagcc tacccgccgg accggcctcg ggcgcgcggg
     2581 tgggcctgct cgtagaccac ccggagccgc tcggccgaga cccgggtgta aacctgcgtc
     2641 gtggcgatcg acgcgtggcc gagcagctcc tgcacgaccc gtatgtcggc gccgtggtcg
     2701 agcatgtggg tggcgcagga gtggcgcagc acgt
//
LOCUS       DASWGG010000226         3152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1501551, whole genome shotgun sequence.
ACCESSION   DASWGG010000226 DASWGG010000000
VERSION     DASWGG010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501551"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..2326)
                     /locus_tag="VF954_04085"
     CDS             complement(119..2326)
                     /locus_tag="VF954_04085"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX9530295.1"
                     /translation="MATVDRVLPWRRQPSPSTNALTPVVTAYRARHPKADTARITRAF
                     ELAATAHSGQFRKSGEEYIHHPTAVAAIVAELGLDDVTIAAALLHDAVEDTGVTVEDI
                     ERDFGAHVSAIVDGVTKLDRVRFDSKEAQQAATMRKMLVAMAKDWRVLIIKLADRLHN
                     MRTIAAMPEWKQKRTAQETLDIYAPLAHRLGIQEVKWQLEDLAFATLHPKRYAEIEQM
                     VATRAPERDIYLTQVLAAVAERLGELRIDAEVTGRPKHLWSIYEKMVVKSREFDEIFD
                     LVGIRVLVDSEKDCWAALGSMHAIWSPVQGRFKDYINTPKFNLYQSLHTTVVGPQGKP
                     LEVQIRTRDMHHRAEFGIAAHWGYKGKASPGEAAWLQRIVDWQQDTPDPAEFLETLKL
                     DLEQDEVYVFTPKGAVLTLPAGATPIDFAYTIHTEVGHRCIGARISGRLVPLDSQLHS
                     GDTVEIFTSKVPNAGPSRDWLKIAVTPRARNKIRQWFSRERRDDAIENGRDELVRALR
                     KEGLPVQKLTSSPMLVKLAESMNYVDLEALHAAIGDGHVSARSVAQRLARELRGGDHD
                     EQLPTTARQPRRPSRRQTVGVYVEGLDDVMIRLSRCCTPVPGDEIVGFVTRGRGVSVH
                     RADCANALSLSSGRNERIIEVEWDRERTGVFTASIEIKALDRARLLADVTHVLSEHHV
                     NIISSSTQVGSDQVSRMRFDFELADPAHLDSMLASLKRIDSVYDGYRILPGRK"
     gene            complement(2334..2864)
                     /locus_tag="VF954_04090"
     CDS             complement(2334..2864)
                     /locus_tag="VF954_04090"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX9530296.1"
                     /translation="MATKVGRLKDLIRDIPDFPRPGVAFKDITPLLADAVAFEEVVEA
                     LSRPWVGAGIDKVLGVEARGFLVAAPVALRLGAGFVPARKAGKLPWQVERQEYALEYG
                     TDLLEIHRDGIAPGETALIIDDVLATGGTAEASIKLVERLGGRAAGLGFVIELGFLGG
                     RLRLGDRPVTSLLSYE"
     gene            complement(2883..>3152)
                     /locus_tag="VF954_04095"
     CDS             complement(2883..>3152)
                     /locus_tag="VF954_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530297.1"
                     /translation="TIRQRLASRGATGSLLTPAAAAAAAGAAASGGGRSTTVLSPRAP
                     ADEVVEDDLGVAATASPGPSPRPRQGPRPGGGRPGPRPRKKGKRR"
BASE COUNT          412 a         1084 c         1171 g          485 t
ORIGIN      
        1 cgcctcgtag cgggccgacg ccggcgggag gacgtcgaag gtgcccttcg gggcctggaa
       61 ggaggggccg ggagccacgc ctggcagact aggccgatcg ctcctgggcc ctggtcgcct
      121 atttcctgcc gggcagtatc cggtacccgt cgtacacgct gtcgatgcgc ttgagcgagg
      181 ccagcatgga gtcgagatgg gccgggtcgg ccagctcgaa gtcgaagcgc atccggctga
      241 cctggtcgga gccgacctgg gtggagctgg agatgatgtt gacgtggtgc tccgacagga
      301 cgtgggtcac gtccgccagc agccgggcgc ggtccagggc cttgatctcg atggaggcgg
      361 tgaacacccc cgtgcgctcg cggtcccact cgacctcgat gatgcgctcg ttgcggccgc
      421 tgctgagcga cagcgcgttg gcgcagtcgg cccggtgcac ggagacccct cgaccccggg
      481 tgacgaagcc gacgatctcg tcgcccggca cgggcgtgca gcagcgggac aggcggatca
      541 tgacgtcgtc gaggccctcg acgtacacgc cgacggtctg gcgccggctg gggcgccggg
      601 gctggcgggc ggtggtgggc agctgctcgt cgtggtcccc gcccctcagc tcccgggcca
      661 gccgctgggc caccgaccgg gccgagacgt ggccgtcgcc gatggcggcg tggagggcct
      721 ccaggtccac gtagttcatg gactcggcca gcttcacgag catgggcgag gaggtgagct
      781 tctggaccgg tagcccctcc ttgcgcaggg cgcgcaccag ctcgtcccgg ccgttctcga
      841 tggcgtcgtc gcgccgctcg cggctgaacc actgccggat cttgttgcgg gccctcggcg
      901 tgaccgcgat cttgagccag tccctcgacg ggccggcgtt ggggacctta gaggtgaata
      961 tctcgacggt gtcgccggag tggagctgcg agtcgagcgg caccaggcgg ccgctgatcc
     1021 gggccccgat gcagcggtgg cccacctcgg tgtggatggt gtaggcaaag tcgatggggg
     1081 tggccccggc cggcaaggtg agcaccgccc ccttcggcgt gaacacgtac acctcgtcct
     1141 gctccaggtc gagcttcagc gtctcgagga actcggcggg gtccggggtg tcctgctgcc
     1201 agtccacgat ccgctggagc caggccgcct cgcccggcga cgccttgccc ttgtagcccc
     1261 agtgggccgc gatgccgaac tcggcgcggt ggtgcatgtc ccgggtgcgg atctgcacct
     1321 cgagcggctt gccctgcggc ccgaccacgg tggtgtgcag cgactggtag aggttgaact
     1381 tgggggtgtt gatgtagtcc ttgaagcggc cctgcacggg gctccagatg gcgtgcatgg
     1441 agccgagggc ggcccagcag tccttctccg agtcgaccag gacgcggatg cccaccaggt
     1501 cgaagatctc gtcgaactcc ctggacttca ccaccatctt ctcgtagatg ctccacaggt
     1561 gcttgggccg gccggtgacc tcggcgtcga tgcgcagctc ccccagccgc tcggccacgg
     1621 ccgccagcac ctgggtcagg tagatgtcgc gctcgggagc ccgggtggcc accatctgct
     1681 cgatctcggc gtaccgcttg ggatggaggg tggcgaaggc caggtcctcg agctgccact
     1741 tgacctcctg gatgccgagg cggtgggcca gcggggcgta gatgtcgagg gtctcctggg
     1801 cggtgcgctt ctgcttccac tccggcatgg cggcgatcgt ccgcatgttg tggagccggt
     1861 cggccagctt gatgatcagg acccgccagt ccttggccat ggccacgagc atcttgcgca
     1921 tggtcgcggc ctgctgggcc tccttggagt cgaagcgaac ccggtccagc ttggtcacgc
     1981 cgtcgacgat ggcggacacg tgggctccga agtcccgctc gatgtcctcc acggtcacgc
     2041 cggtgtcctc cacggcgtcg tgcagcaggg cggccgcgat ggtgacgtcg tccagcccga
     2101 gctcggccac gatggccgcc accgccgtcg gatggtggat gtactcctcg cccgacttgc
     2161 ggaactgccc agagtgggcg gtcgcggcca gctcgaaggc ccgggtgatg cgggcggtgt
     2221 cggccttggg atgccgggcc cggtaggcgg taacgacggg cgtcaaggcg ttggtggacg
     2281 gggagggctg gcggcgccag ggcagcaccc ggtcgacggt ggccatcggc gccctactcg
     2341 tagctcagca gcgaggtgac ggggcggtcg ccgagccgga gccggccgcc gaggaagccc
     2401 agctcgatca cgaagccgag gcccgcggcc cgcccgccga ggcgctcgac cagcttgatg
     2461 ctggcctcgg ccgtcccgcc ggtggccagc acgtcgtcga tgatcagcgc cgtctcgccc
     2521 ggggcgatcc cgtcgcggtg gatctcgagg aggtcggtgc cgtactcgag cgcgtactcc
     2581 tggcgctcga cctgccaggg cagcttgccc gccttccgcg ccggcacgaa gccggcgccc
     2641 agccgcaggg cgaccggtgc ggccaccagg aagcctcgtg cctcgacccc cagcaccttg
     2701 tcgatgccgg cgcccaccca gggccgggac agggcctcga cgacctcctc gaaggcgacc
     2761 gcgtcggcca gcagcggggt gatgtccttg aacgcgaccc cgggccgggg gaagtcaggg
     2821 atgtcccgga tgagatcctt gagacgcccg accttcgtcg ccatgcatcg aagataccgc
     2881 ccctagcgcc gcttgccctt cttcctcggc ctcggcccgg gccgtccgcc gccggggcgg
     2941 ggcccctggc ggggccgggg cgaaggcccg ggcgaggccg tcgcggccac gcccaggtcg
     3001 tcctcgacca cctcgtcagc gggggccctc gggctgagca cggtcgtgga ccgcccgccg
     3061 ccggacgccg ccgcgccggc cgcagccgcg gccgccgcgg gggtcaggag gctcccggtg
     3121 gcgccgcgcg aggccagccg ctggcggatg gt
//
LOCUS       DASWGG010000227         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3117095, whole genome shotgun sequence.
ACCESSION   DASWGG010000227 DASWGG010000000
VERSION     DASWGG010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3117095"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /gene="ispG"
                     /locus_tag="VF954_04100"
                     /gene_synonym="gcpE"
     CDS             complement(<1..1102)
                     /gene="ispG"
                     /locus_tag="VF954_04100"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX9530298.1"
                     /translation="MQAPASVTFERRPTRQIHLGAVPVGGGAPITVQSMTITKTADVD
                     GTLAQIYALAGAGADIVRCTCNTEDAAEGLARIVPRSPVPIVADVHNNWRMGIAALEA
                     GVQCLRLNPGNIRNPAHIKQIASMAKDLGVPIRIGVNAGSLDSKLYQKHGGATPEAMV
                     ESAQMELAYLDEVGFEDVKISVKASNVPLMVEAYRQLADATDHPLHLGVTEAGPPPAG
                     LVKATAGMATLLAEGIGDTIRYSLTADPVEEARAGRMLLEALGLRARKGVDLIACPSC
                     GRAEIDVIKVANDALAAFGEREIPLQVAVMGCVVNGPGEARHADLGIAAGRRKGHLFI
                     KGQNVAVVPEAEMVEALVHYAELIMAEGTDTVL"
     gene            complement(1106..>1619)
                     /locus_tag="VF954_04105"
     CDS             complement(1106..>1619)
                     /locus_tag="VF954_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX9530299.1"
                     /translation="GVEMHLGLSTANPVAAVGTAATQLGSTIWLTMKALGGIFSPHGM
                     SAYANQLTGNPPTGASAANAPRFLSPVGFVRVASQAAHTGLYDVLNLLILINIFVGVF
                     NLVPLLPLDGGHVAIAVYEAARSRRGRRYHADVAKLLPLTYLVLLTIVFLGATSLYLD
                     IVHPLANPFR"
BASE COUNT          228 a          510 c          637 g          244 t
ORIGIN      
        1 cgaggacggt gtcagtgccc tcggccatga tcagctcggc atagtgcacc agggcctcga
       61 ccatctcggc ctcggggacg acggcgacgt tttggccctt gatgaagagg tggcccttgc
      121 gccgcccagc cgcgatgccg aggtcggcgt ggcgcgcctc gcccgggccg ttgaccacgc
      181 agcccatgac cgcgacctgg agcgggatct cccgttcgcc gaaggcggcc agggcgtcgt
      241 tggccacctt gatcacgtcg atctcggccc ggccgcagga ggggcaggcg atgaggtcca
      301 cccccttgcg ggcgcgcagg cccagggctt cgagcagcat gcggccggcg cgggcctcct
      361 cgacggggtc tgccgtgagc gagtagcgga tggtgtcgcc gatgccctcc gccagcagcg
      421 tcgccatgcc cgccgtcgcc ttgaccaggc cggccggcgg gggcccggcc tcggtcacgc
      481 cgagatggag ggggtggtcg gtggcgtcgg ccagctggcg gtaggcctcg accatgagcg
      541 gcacgttgga ggccttgacc gagatcttga cgtcctcgaa gccgacctcg tcgaggtagg
      601 ccagctccat ctgggccgac tccaccatgg cctccggcgt ggctccgccg tgcttctggt
      661 agagcttgga gtcgagcgag ccggcgttga cgccgatccg gatgggcacg cccaggtcct
      721 tggccatcga ggcgatctgc ttgatgtggg ccgggttgcg gatgttgcca gggttgagcc
      781 ggaggcactg cacgcccgcc tccagcgcgg cgatgcccat gcgccagttg ttgtggacgt
      841 cagccacgat cggcacgggc gagcggggca cgatgcgggc caggccctcg gctgcgtctt
      901 cggtgttgca ggtgcagcgc acgatgtcgg cgccggcgcc ggcgagggcg tagatctggg
      961 ccagggtgcc gtccacgtcc gcagtcttgg tgatggtcat ggactgcacg gtgatcggcg
     1021 cgccgccgcc caccggcacc gcgccgaggt ggatctggcg ggtggggcgg cgctcgaagg
     1081 tgacgctggc gggagcctgc acgagctacc ggaaggggtt ggccaggggg tgcacgatgt
     1141 ccaggtacag cgacgtggcc ccgaggaaga cgatggtgag caggacgagg tatgtcaagg
     1201 gcaggagctt ggccacgtcc gcgtggtaac ggcggccccg ccgggagcgg gcggcctcgt
     1261 agacggcgat ggccacgtgg ccgccgtcga ggggcagcag cgggaccagg ttgaacacgc
     1321 cgacgaagat gttgatcagg atcagcaggt tgagcacgtc gtagagcccg gtgtgggccg
     1381 cctgggacgc cacccgaacg aagcccaccg gcgacaggaa cctgggggcg ttggccgctg
     1441 atgcgccggt gggagggttg ccggtgagct ggttggcgta cgccgacatg ccgtgcgggg
     1501 agaagatccc gccgagggcc ttcatggtca gccagatggt gctgccgagc tgcgtggcgg
     1561 ccgtgcccac cgcggcgacg gggttggcgg tgctgaggcc gaggtgcatc tcgacgccg
//
LOCUS       DASWGG010000228         6092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2358681, whole genome shotgun sequence.
ACCESSION   DASWGG010000228 DASWGG010000000
VERSION     DASWGG010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358681"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..628)
                     /gene="folK"
                     /locus_tag="VF954_04110"
     CDS             complement(185..628)
                     /gene="folK"
                     /locus_tag="VF954_04110"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX9530300.1"
                     /translation="MFLGLGSNLGDRLQHLRDAVASLPDVVAVSPVYETDPVGGPLGQ
                     DPYLNLVVELQTDLSARQLLGISHRLESAAGRVRTERWGPRPLDVDLLLVGDATISEP
                     DLVVPHPRMWERRFVLAPLADLAPELVPAGWERRVAGGARMAGTL"
     gene            complement(631..1002)
                     /gene="folB"
                     /locus_tag="VF954_04115"
     CDS             complement(631..1002)
                     /gene="folB"
                     /locus_tag="VF954_04115"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX9530301.1"
                     /translation="MDRIELRGLRQVGTHGLLPEEQTRAQPFEVDLDVELDLEAAGRS
                     DDLGATVDYGALAELAGRVIGEERHGLLERLAQRIAEVVLADRRVVAVSVTVRKLRPP
                     VAADLASAGVHIRRARPGAPS"
     gene            complement(1006..1500)
                     /locus_tag="VF954_04120"
     CDS             complement(1006..1500)
                     /locus_tag="VF954_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530302.1"
                     /translation="MRGKWAAGIPPRFFSWIIKDQLAISERPGGYARNHRRVRRQEEI
                     IWIREQGFTKVVSLLASPHNLHAYDELGVRWAHFPFGPQQDTAAVLPELYTGLRSWLG
                     AGDRVLLHQEELSDKLMGVVAGYLLWSRKLPDGPQAIMVVERIVGRQMGPPGRELVGA
                     VPEG"
     gene            complement(1497..2303)
                     /gene="folP"
                     /locus_tag="VF954_04125"
     CDS             complement(1497..2303)
                     /gene="folP"
                     /locus_tag="VF954_04125"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9530303.1"
                     /translation="MLAQVMGVLNVTPDSFSDGGRWFDHRAAIAHGLDMAAQGADVVD
                     VGGESTRPGARPVDEMEECRRVVPVVEALARRVRVSVDTSKPGVAKAAVAAGATLLND
                     VTATLGAVAADLGVGWVAMHMQGSPATMQACPSYADVVLEVRDYLEERAGAARAAGVA
                     EVWIDPGIGFGKTPAHNLALLRHLRVLVDTGFPVLVGASRKSFLGRVAAGPAEPALGV
                     GDRREASLAAATVALVNGAAMVRAHDVAPTVQAARIVGQPVAPAARGAGR"
     gene            complement(2373..2948)
                     /gene="folE"
                     /locus_tag="VF954_04130"
     CDS             complement(2373..2948)
                     /gene="folE"
                     /locus_tag="VF954_04130"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX9530304.1"
                     /translation="MGIDQARVERAVREILEAIGEDPDRDGLQATPSRVARMYAEVLG
                     GRDEDPGCHLTVTFEANHDEMVMVSPIAFASTCEHHMVPFVGEAHIAYIPNDDGRITG
                     LSKLARLVDAHARRLQVQERLTTAIADSIEAVLAPRGVLVVVQAEHFCMTVRGVKKPG
                     TRATTSAVRGLFRDDARTRAEAMAFIHSQKS"
     gene            complement(2962..5034)
                     /gene="ftsH"
                     /locus_tag="VF954_04135"
     CDS             complement(2962..5034)
                     /gene="ftsH"
                     /locus_tag="VF954_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX9530305.1"
                     /translation="MKRGLRNILIYAALGVVVLIVVTSLLGGGPGREKASLTRLDGWI
                     NDRAVVHAVVVDGDNKVNYELKSGPTRYQTSYPDRYTPDLTARLLSSGAVVESKKHHA
                     NFWLSLLGSLLPFALFLGGFVFILNTVQGGGNRVMQFGKAKPKTVSKDQPKVTFADVA
                     GVDEAVDELQEIKEFLEAPAKFQAIGAKIPKGVLLFGPPGTGKTLLARAVAGEAGVPF
                     FSISGSDFVEMFVGVGASRVRDLFEQAKSSAPAIIFMDEIDAVGRHRGAGLGGGHDER
                     EQTLNQLLVEMDGFDLKTGVILIAATNRPDILDPALLRPGRFDRQIVVDRPDLEGRKA
                     ILRVHAAGKPVSSLVELDVLARRTPGFTGADLANLMNEAALLAARHGHSEISMGELEE
                     AIDRVIAGPERKTRVMSEREKLVIAYHEGGHALVGHVLPQADPVHKVSIVARGRALGW
                     TLSLPTEDKYIVTRSELTQQLAVLMGGRTAEEMVFADPTTGAANDIEKATEIARAMVT
                     QYGMSEVLGPQQLGQQTAEVFLGRDLGHQANYSDDMAARIDDEMRALIDSAHDEAAAI
                     LARHRETLDRLAAALMEKETLDTAELMAILGPLPVWSGQGRRGGNGTRRPPAGRGSRR
                     AAGEQAGSAATAAVTPPPAKPSVAAAAPPARRPKGTGRAARSSAAPAQASAARATPAR
                     AGRRPSPS"
     gene            complement(5075..5626)
                     /gene="hpt"
                     /locus_tag="VF954_04140"
     CDS             complement(5075..5626)
                     /gene="hpt"
                     /locus_tag="VF954_04140"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX9530306.1"
                     /translation="MVPTDPTIGRVVVGEKDLAHRVVEMGEEITADYAGQRPLLVGVL
                     KGAFVFMSDLARAVRLPVEFDFMAVASYGSATKTSGVVRIVKDLDLDLTGRDVIIVED
                     IVDSGLTLSFLRRNLMARNPASLEVCALLVKEGLQRGDPDLRYVGFRIPPDFVIGYGL
                     DVGERYRNLRSVHVYQPDGASVP"
     gene            complement(5636..>6092)
                     /locus_tag="VF954_04145"
     CDS             complement(5636..>6092)
                     /locus_tag="VF954_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530307.1"
                     /translation="DDPSNRDPAHLRNRVRHELLPLMGALAGRDPVPVLVRQAELFRA
                     EAGVLEGLAAAIPVEVASDLAAAPVALSRRRVRQWLRRDGARPAYPPDGATVERVLAV
                     ARLEARACEIGGGLRVRRSGGRLRLEGQGLEGRGLGETRLEGRASTPPR"
BASE COUNT          824 a         2208 c         2238 g          822 t
ORIGIN      
        1 ccagtcacac ccgcgggccg ctccagagag gtcgtcgccc cgccccaggg gaggcagcac
       61 gcgccagccg gacgcagcca gcgcgctggc gaaggaacgc ccggcgcggc cgggcccgat
      121 gagccgcagg gtggtcacaa ggtctcgatt cgcgttccgg cccctggtgg gtgccgttcg
      181 tgatctacag cgtacccgcc atccgggccc cgcccgcaac ccgacgctcc cagccggcgg
      241 ggacgagctc cggggccagg tctgccagcg gtgccagcac gaagcggcgc tcccacatcc
      301 gcgggtgggg cacgacgagg tcgggctcgg agatggtggc gtcgccgacg aggaggaggt
      361 cgacgtcgag ggggcgcggc ccccatcgct cggtacgcac ccgaccggcc gccgactcca
      421 ggcggtgcga gatgcccagc agctgccggg cggacaggtc ggtctggagc tcgacgacca
      481 ggttgaggta cgggtcctgg cccaggggcc cgcccaccgg gtcggtctcg tacacgggcg
      541 agactgcgac cacgtcgggg agggaggcga cggcatcgcg caggtgctgg aggcgatcgc
      601 cgaggttgga ccccaacccg aggaacaccc tcacgacggc gcgccgggcc gggcccggcg
      661 gatgtggacg ccagccgacg ccaggtcggc ggccacgggc gggcggagct tgcggaccgt
      721 caccgacacg gccaccaccc gccggtcggc gagcaccacc tcggcgatgc gttgggccag
      781 ccgctccagg aggccgtggc gctcctcgcc gatcacccgg ccggcgagct ccgcgagcgc
      841 gccgtagtcc accgtggcgc ccagatcgtc gctgcggccc gcggcttcga gatcgagctc
      901 gacgtcgagg tcgacctcga agggctgggc cctcgtctgc tcctcgggca gcaggccgtg
      961 cgttccgacc tgccggaggc cacgcagctc gatgcggtcc acggctcagc cctcggggac
     1021 ggcgccgacc agctcgcgcc cgggggggcc catctgccgg cccacgatgc gctcgacgac
     1081 catgatggcc tgcggcccgt cgggcagctt gcggctccag agcaggtagc cggccaccac
     1141 ccccatcagc ttgtcgctga gctcctcctg gtggagcagg acccggtcgc cggccccgag
     1201 ccaggagcga aggccggtgt aaagctcggg caggacggcc gcggtgtcct gctggggccc
     1261 gaacgggaag tgggcccagc gcacgccgag ctcgtcgtag gcgtgcaggt tgtggggcga
     1321 agccaggagg gatacgacct tggtgaagcc ctgctcacgg atccagatga tctcctcctg
     1381 ccggcgcacc cgccggtggt tgcgggcgta gccgcccggg cgctcgctga tggccagctg
     1441 gtccttgatg atccaggaga agaaccgggg cgggatcccg gccgcccact tgcctctcaa
     1501 cggccggccc cacgggcggc gggcgcgacg ggctggccca cgatgcgggc cgcctggacg
     1561 gtgggcgcca cgtcgtgggc gcggaccatg gcggccccgt tgaccagggc gaccgtggcc
     1621 gccgccagcg acgcctcgcg ccggtccccc acccccaggg ccggctcggc cggcccggcc
     1681 gccacccggc cgaggaagct cttacggctg gcgccgacca gcaccgggaa cccggtgtct
     1741 accagcaccc ggaggtggcg caggagggcc aggttgtggg ccggggtttt tccgaagccg
     1801 atccccgggt cgatccacac ctccgccaca ccggcggcgc gcgccgcgcc ggcgcgctcc
     1861 tccaggtagt cccggacctc gagcaccacg tccgcgtagc tgggacaggc ctgcatggtg
     1921 gcgggcgagc cctgcatgtg catggcgacc cagcccacgc cgagatcggc cgccacggcg
     1981 cccagggtgg ccgtcacgtc gttcagcagg gtggcgccgg ccgccaccgc cgccttggcc
     2041 acgcccggct tgctggtgtc gaccgacacg cgcacgcgcc gggcgagcgc ctccaccacc
     2101 ggcaccaccc gccggcactc ctccatctcg tcgaccgggc gggcgccggg gcgggtcgac
     2161 tcgccgccca cgtccaccac gtcggcgccc tgggcggcca tgtcgagacc gtgggcgatg
     2221 gcggcccggt ggtcgaacca ccgtccgccg tcagagaagg agtccggcgt cacgttgagg
     2281 acgcccatga cctgggcaag catcgacgcc cagcgtatcg gccggcggtt tacggccccg
     2341 gggccgtggc ccggggccgt gccgcggggc cactacgact tctgcgaatg gatgaaagcc
     2401 atggcctccg cccgcgtccg ggcgtcgtcg cggaacagcc cccggacggc ggacgtggtg
     2461 gcccgggtgc cgggcttctt cacgccccga acagtcatgc agaagtgctc ggcctggacc
     2521 acgaccagca cgcccctggg ggcgaggacg gcttcgatgc tgtcggcgat ggcggtcgtg
     2581 agccgctcct ggacctggag ccggcgggca tgggcgtcta ccagccgggc cagcttggac
     2641 aggccggtga tccggccgtc gtcgttgggg atgtaggcga tatgggcctc gcccacgaac
     2701 ggcaccatgt ggtgctcgca cgtgctggcg aacgcgatgg gcgagaccat caccatctcg
     2761 tcgtggttgg cctcgaaggt gaccgtcagg tggcaccccg ggtcctcgtc ccggccgccc
     2821 aggacctcgg cgtacatcct ggccaccctg gaaggggtgg cctggagccc gtcgcggtcg
     2881 gggtcctcgc cgatggcctc gaggatctcc cgcacggccc gttcgaccct ggcctggtca
     2941 atgcccatta cgagagtccc atcacgaggg cgagggccgg cggccggccc gggccggggt
     3001 ggctcgggcg gcagacgcct gggccggggc tgccgacgac cgagcggccc ggccggtccc
     3061 cttgggccgg cgggcgggcg gcgcggcggc ggccaccgac ggcttggcgg gcggcggcgt
     3121 cactgccgcc gttgcggccg agcccgcctg ctccccggcg gcgcggcgcg acccgcggcc
     3181 ggcgggcggg cgccgggtcc cgttcccgcc ccgccgcccc tggccgctcc agacgggcag
     3241 ggggccgagg atggccatga gctcggcggt gtcgagcgtc tctttctcca tgagggcggc
     3301 cgcgaggcga tccagcgtct cccggtgcct ggccaggatg gcggccgcct cgtcgtgggc
     3361 ggaatcgatc agggcgcgca tctcgtcgtc gatgcgggcc gccatgtcgt cggagtagtt
     3421 ggcctggtgg ccgaggtccc ggcccaggaa cacctcggcg gtctgctggc cgagctgctg
     3481 gggaccgagc acctcgctca tcccgtactg ggtcaccatg gcccgggcga tctcggtcgc
     3541 cttctcgatg tcgttggccg cgcccgtcgt cggatcggcg aacaccatct cctcggccgt
     3601 ccggccgccc atcagaacgg cgagctgctg ggtgagctcg gagcgggtca cgatgtactt
     3661 gtcctcggtc ggcagtgaca gcgtccagcc cagggctcgg ccccgggcga cgatggagac
     3721 cttgtggacc gggtcggcct gaggcaggac gtggccgacg agggcatggc ccccctcgtg
     3781 gtacgcgatg accagcttct cccgctctga catcacccgg gtcttgcgct cggggcccgc
     3841 gatcacccgg tcgatggcct cctcgagctc gcccatgctg atctcggaat ggccgtggcg
     3901 ggcggcgagg agggcggctt cgttcatcag attggccagg tcggcgcccg tgaagcccgg
     3961 ggtccggcgg gccaggacgt cgagctccac cagggagctg accggcttgc cggcggcatg
     4021 gacgcgcagg atggccttgc gcccctccag gtccgggcgg tcgaccacga tctggcgatc
     4081 gaatcggccc gggcgcagca gggccgggtc caggatgtcg ggccggttgg tggccgcgat
     4141 gaggatgacg ccagtcttga ggtcgaaccc gtccatctcg accagcagct ggttgagggt
     4201 ctgctcccgc tcgtcgtggc ccccgcccag gccggcgccg cggtggcgcc cgacggcgtc
     4261 gatctcgtcc atgaagatga tggcgggagc cgaggacttg gcctgctcga acaggtcgcg
     4321 aacccggctg gcgccgacgc cgacgaacat ctccacgaag tcggagcccg agatggagaa
     4381 gaacggcacg ccggcctcgc cggccacggc gcgggccagc agggtcttgc ccgtccccgg
     4441 cggcccgaac agcagcacgc ccttggggat cttggccccg atggcctgga acttggccgg
     4501 ggcctccagg aactccttga tctcctggag ctcgtccacc gcctcgtcga cccccgccac
     4561 gtcggcgaag gtcaccttgg gctggtcctt tgagacggtc ttgggcttgg ccttgccgaa
     4621 ctgcatgacc cggttgccgc cgccttgcac ggtgttgagg atgaagacga agccgcccag
     4681 gaacagggcg aacggcagca ggctccccag caggctgagc cagaagttgg cgtggtgctt
     4741 cttggactcg accaccgcac cgctggacag aagcctggcg gtcaagtcgg gcgtgtaccg
     4801 gtccgggtag ctggtctggt accgggtcgg gccgctcttg agctcgtagt tgaccttgtt
     4861 gtcgccgtcc accaccacgg cgtggaccac ggcgcggtcg ttgatccagc cgtcgagcct
     4921 ggtgagcgag gccttctccc ggccgggacc cccgccgagc aaggaggtga ccacgatgag
     4981 gaccacgacg ccgagcgcgg cgtagatcaa gatgttgcgc aggccgcgct tcacgaggcc
     5041 gccgggaacg gggtgcggga tcgcatgacc ttgcctaggg taccgacgcc ccgtccggtt
     5101 ggtacacgtg gaccgatcgc agattgcggt agcgctcgcc cacgtccagc ccgtagccga
     5161 tcacgaagtc gggtgggatg cggaacccga cgtagcgcag atcggggtcg ccccgctgga
     5221 ggccttcctt gacgagcagg gcgcacacct ccaggctggc cgggttgcgc gccatcaggt
     5281 tgcgccggag gaacgacagc gtcagcccgc tgtcgacgat gtcctccacg atgatcacgt
     5341 cccggccggt gaggtccagg tcgaggtcct tgacgatgcg caccacgccg ctcgtcttgg
     5401 tggcgctccc gtaggacgcc acggccatga agtcgaactc gacggggagc cgcaccgccc
     5461 gggccaggtc gctcatgaag acgaacgccc ctttgagcac gcccacgagc aggggccgct
     5521 ggccggcgta gtcggcggtg atctcctcgc ccatctcgac cacccggtgg gcgagatcct
     5581 tctcgcccac cacgacccgg ccgatggttg gatccgtcgg gaccacgctg cgaccctatc
     5641 ggggtggggt cgaggcccgc ccctccaggc gcgtctcccc caggccccgt ccctcgaggc
     5701 cctgcccctc caggcggagg cggccgcccg aacgccgcac ccgaaggccg ccgccgatct
     5761 cgcacgccct cgcctccagc cgggccacgg ccagcacccg ttctaccgtg gccccgtcgg
     5821 gcgggtaggc cggccgggcg ccgtcgcggc gcagccactg ccgcacgcgc cggcggctca
     5881 gggcgaccgg ggccgcggcc aggtcggagg ccacctcgac cgggatggcc gcggccaggc
     5941 cctcgagcac accggcctcg gcgcggaaca gctccgcctg ccggaccagc actggtaccg
     6001 ggtcccgacc ggccagcgcg cccatcaggg gcagcagctc gtggcggacc ctgttgcgga
     6061 gatgggcggg atcgcggttg gacgggtcgt cg
//
LOCUS       DASWGG010000229         3325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1188772, whole genome shotgun sequence.
ACCESSION   DASWGG010000229 DASWGG010000000
VERSION     DASWGG010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188772"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VF954_04150"
     CDS             <1..1254
                     /locus_tag="VF954_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530308.1"
                     /translation="DAAQLTSPTLPADTFATAMAPHGADGLWVDDARGDIFNLGSAPP
                     VGSALAPNQPVTGLAANHAGTGWWAVDADGLVSASSATTVVGDPYIVPTSGGLARLAF
                     TSVALGTSIVSASAGGVSSAPANVQWMPPPSGYWLGAQDGGIFAFGIARFRGSTGAMR
                     LNQPIVGMARTPTGLGYWLVARDGGIFAFGDAGFFGSTGGMHLNKPIVGMAATLDGRG
                     YWLVASDGGIFSFGDARFLGSTGSIHLNQPIVGMAPGFLGFGYWLVASDGGIFAFGDA
                     GFHGSTGSIALNKPIVAMAPTLFGDGYWLVASDGGIFAFGDAGFHGSTGSIHLNQPIV
                     GMAPTAVDDGYWLVARDGGIFAFGTAEFNGSTGSITLNQPIVGMAAIQPFYVPGPISF
                     GSGGRGGSGVRSPNVELARLARGDR"
     gene            complement(1461..1757)
                     /locus_tag="VF954_04155"
     CDS             complement(1461..1757)
                     /locus_tag="VF954_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530309.1"
                     /translation="MRLNHIVAGVLLVGGVGLSAGAAGAATNPSTTGQPSQSCQAQPS
                     SPGQASSSPGAPFNEPSATSPGGTGGAAYNAGNANSPNPKAVSQYDVACYQVSH"
     gene            complement(1832..2671)
                     /locus_tag="VF954_04160"
     CDS             complement(1832..2671)
                     /locus_tag="VF954_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9530310.1"
                     /translation="MLSRRGFLIGVGAAGAVAVGGYVEAGASERRRLLHHLGLAASPD
                     HRVPPSATRVVSGKLDSTFMRRTVGWSVSMPDPPLAGVVYCLHGKGDDHRFAFDTIHL
                     HDVAAAQGARLAVAAVDGGADSYWHPRADGTDALGMLVHEFVPMVEREVGPLARRALI
                     GWSMGGYGALLVAETTPQTFSAVAAASPALWLSAAATAPGAFDDAEDYRRHDVVAGEA
                     RLARLVVRVDCGQGDPFYRAARSFAAGLPGPPQTSFGPGYHDAAYWRSVAPAQIATIM
                     GVT"
     gene            2775..3044
                     /locus_tag="VF954_04165"
     CDS             2775..3044
                     /locus_tag="VF954_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530311.1"
                     /translation="MIVPAGDDARPSAMERELVLASVVEAGVVTVAEVAADTGFRPTR
                     VREALLRLAIDDAVVSDGERWRPGRGARQPARRHASQAGGLAGSA"
     gene            complement(3060..>3325)
                     /locus_tag="VF954_04170"
     CDS             complement(3060..>3325)
                     /locus_tag="VF954_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530312.1"
                     /translation="AEVGIDISREFPKPWTDEIVRAADVVVTMGCGDACPFFPGHRYE
                     DWELEDPAGRPLEEVRPIRDEIGRRVRDLLARLYVPVDVPVTE"
BASE COUNT          444 a         1190 c         1204 g          487 t
ORIGIN      
        1 gacgccgccc agctgacgtc accgacgctg ccggccgaca ccttcgccac ggcgatggcg
       61 ccgcacggcg ccgacgggct ctgggtggac gacgcgcgcg gggacatctt caacctcggg
      121 agcgcgcccc cggtcggctc ggcgctggct cccaaccagc cggtgaccgg gctggcggcc
      181 aaccatgccg gcaccgggtg gtgggccgtg gatgccgacg gtctggtgtc ggcctcgagc
      241 gcgacgacgg tggtcggcga tccctacatc gttcccacgt cgggcgggct ggcccggctg
      301 gcgttcacgt cggtcgccct cgggacctcg atagtctcgg cctccgccgg cggggtcagc
      361 tcggctccgg cgaacgtgca gtggatgccg ccgccgagcg gctactggct gggggcccag
      421 gacggcggca tcttcgcctt tgggatcgcc cgcttccgcg ggtccacggg tgcgatgcgg
      481 ctcaaccagc ccatcgtggg catggctcgc acgcctaccg gtctgggcta ctggctggtg
      541 gcccgggacg gcggcatctt cgccttcggc gacgccgggt tcttcggctc gaccggcggg
      601 atgcacctca acaagcccat cgtcggcatg gcggccaccc tcgacggcag gggctactgg
      661 ctggtggcca gcgacggcgg catcttctcc ttcggcgacg ccaggttcct cggctccacc
      721 gggtccatcc acctcaacca gcccattgtc ggcatggcgc ccggcttctt ggggttcggt
      781 tactggctgg tggccagcga cggcggcatc ttcgcattcg gcgacgccgg cttccacggc
      841 tccaccggga gcatcgcgct caacaagccc atcgtggcca tggcgcccac cctcttcggt
      901 gacggctact ggcttgtggc cagcgacggc gggatcttcg ccttcggcga cgcgggcttc
      961 cacggctcca ccgggtccat ccacctcaac cagcccatcg tcggcatggc cccgaccgcg
     1021 gtcgacgacg ggtactggct ggtggcccgg gacggtggca tcttcgcctt cggcacggcc
     1081 gagttcaacg ggtccacggg gtccatcacg ctgaaccagc ccatcgtcgg gatggcggcc
     1141 atccagccct tctacgtccc cgggccgatc tcgttcgggt cgggcgggag gggcgggtcc
     1201 ggcgtgcgat cccccaatgt cgagctggcc cggctggcgc gaggcgaccg gtagggcgct
     1261 ccgctcacgg acggtgcagg attctttcgg tggcgaggtg gcgtcagagc cggctcggca
     1321 aggtgtcgag agatagctgg ggagattagg tggtctcaga ccacctaatc tccccagcaa
     1381 catgaaataa catgaaaagg gagccaacct cccaggtcct cgggccgagc acttgtccag
     1441 ccgttgctta gggtggccga tcagtgcgag acctggtagc aggccacgtc gtactgggag
     1501 acggccttgg ggttgggtga gttggcgttg ccggcgttgt aggcggcgcc tcccgtccct
     1561 ccggggctgg tggccgacgg ctcgttgaac ggtgcacccg gagacgagct ggcctgcccg
     1621 gggctggacg gctgcgcctg gcaggactgc gacggctggc ccgtggtgct ggggttggtg
     1681 gccgcgcccg cggcgccggc cgacaggccg acacctccga cgagcaggac gccggcgacg
     1741 atgtggttga gtctcatacc aggcatatac gaccccgccg gccggaggga tggcgtccgg
     1801 tgcccgcgcc tttcttccac gtccccgatc ctcatgtgac gcccatgatc gtggcgatct
     1861 gcgcgggtgc gacgctcctc cagtaggcgg cgtcgtggta gccaggcccg aagctcgtct
     1921 gcggcggtcc gggcagcccg gcggcgaagc tgcgggcggc ccggtagaag gggtccccct
     1981 ggccgcagtc gacccgcacc acgaggcggg ccagccgggc ctcgccggcc accacgtcgt
     2041 ggcggcggta gtcctcggcg tcgtcgaagg caccgggcgc ggtggccgcc gccgagagcc
     2101 acagggccgg gctggcggcg gcgaccgcag agaaggtctg cggggtggtc tcggccacga
     2161 gcagggcgcc gtagccgccc atcgaccagc cgatcagggc ccggcgagcc aggggcccga
     2221 cctcgcgctc caccatcggc acgaactcgt gcaccagcat cccgagcgca tctgttccgt
     2281 cggcccgggg atgccagtag gagtccgcgc ccccgtcgac cgcggccacc gccagccggg
     2341 cgccctgggc cgcggccacg tcgtggaggt ggatggtgtc gaacgcgaac cggtggtcgt
     2401 cgcccttgcc gtgcaggcag tacacgacgc cggccaacgg gggatcgggc atcgagacgc
     2461 tccagcccac cgtccggcgc atgaaggtgc tgtccagctt gcccgagacc acccgagtgg
     2521 cggagggagg cacgcggtgg tcggggctgg cggccaggcc gagatggtgc agcaggcgcc
     2581 gacgctcgct ggccccggcc tcgacgtagc cgcccaccgc gaccgcgccg gccgcgccca
     2641 cgccgatgag gaagcccctg cggctcaaca tcgcaacagt gtgccgccca cgcgccgacg
     2701 ccggcggctc cctggcgggc cgccggcgtc acagggccac gcggcggcca ctgcgcccgc
     2761 gtagggtgat cgacatgatc gttcccgcgg gagacgatgc caggccttcg gccatggaaa
     2821 gggagctggt gctcgcttcg gtggtcgagg ccggcgtcgt cacggttgcc gaggtggccg
     2881 ccgacacggg ctttcgaccc acccgggtac gcgaggccct gctccggctc gccatcgacg
     2941 atgccgtcgt ctccgacggt gagcgatggc ggcccgggcg cggcgcccgt caacctgccc
     3001 gccgccatgc gtcgcaggcc ggtgggctgg ccggctccgc gtagccacct gccggggagc
     3061 tactccgtca cgggcacgtc tacgggcacg tacaaccggg cgaggaggtc gcgcacccgc
     3121 cggccgatct cgtcccgtat ggggcggacc tcctcgagcg gccgcccggc gggatcctcc
     3181 agctcccagt cctcgtagcg atgcccggga aagaacgggc acgcgtcgcc gcagcccatc
     3241 gtcaccacga cgtccgcggc ccgcacgatc tcgtcagtcc acggcttggg gaattcgcgg
     3301 gagatatcga tgcccacctc ggcca
//
LOCUS       DASWGG010000230         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2040510, whole genome shotgun sequence.
ACCESSION   DASWGG010000230 DASWGG010000000
VERSION     DASWGG010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040510"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VF954_04175"
     CDS             complement(<1..372)
                     /locus_tag="VF954_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9530313.1"
                     /translation="MPTKSQSKPQAGTSPAPALAPYRVLDLTTAEGWLCGALLADLGA
                     DVVKVEPPGGDPGRAIGPFAGDRATDPDASLAWWFGNRGKRSVVIDTGAAAGRERLDR
                     LIERADVVLESPGEAGRTARLV"
     gene            475..1617
                     /locus_tag="VF954_04180"
     CDS             475..1617
                     /locus_tag="VF954_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9530314.1"
                     /translation="MSGFELSEEHEQLRRVVRDFAEARLAPNVGRWDEQQVFPVDAVL
                     AMGELGLFGLVFPPEWGGSGGDFTSLCVAVEEIGRVDQSLGITLSAGVGLGANPIYRF
                     GDDEQRAKWLPDLVAGRALGAFALTEPDGGSDAGAARSRARRDGDGWVLDGSKAFITN
                     SGTAITSVVTVTARTDVGISAFLVPAGTPGLVVEPPYRKLGWHASDTHGLVLDGCRLP
                     AESLLGEPGAGLHNLLSILDDGRIAISALALGCARACLELSVGYARDREAFGGPIGRF
                     QAIAFALSDVDVAVENARNLTYKAAWLKDAGRPVGRAAAMAKLYSTEAAVAASRVATQ
                     VFGGAGFMEETPVARFYRDAKVLEIGEGTSEIQRLVIARSLGLPVG"
     gene            1679..>2333
                     /locus_tag="VF954_04185"
     CDS             1679..>2333
                     /locus_tag="VF954_04185"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9530315.1"
                     /translation="MPEVLGHVDDGWGKVADAFRSNLEDHDLGAAFCAEVGGRRVVDV
                     WGGVADARTGRPWQEDTIAVVFSVTKGAVAICANRLVERGELDLDEPVATYWPEFAAH
                     GKGAIPVRWLLTHQAGLPVVDVPLTVQDVCAWYPVIRALEAQRPLWEPGSRHAYHAHT
                     FGFLVGEVVRRAHGRTVGAMFAGDVARPLGLSAWIGLPEGEEDRVAHLRAAPPAPPEM
                     "
BASE COUNT          295 a          798 c          899 g          341 t
ORIGIN      
        1 gaccagccgg gcggtgcgcc ccgcctcgcc gggcgactcc agcaccacgt cggcgcgctc
       61 aatgaggcgg tcgaggcgct cccgaccggc cgcggcgccc gtgtcgatga cgacggagcg
      121 cttgccgcga ttgccgaacc accaggccag gctggcatca gggtcggtgg cgcgatcacc
      181 ggcgaacggg ccgatcgccc ggccggggtc gccgccgggg ggctccacct tcacgacgtc
      241 ggcccccaga tcggcgagca gggcgccgca cagccaaccc tcggcggtgg tgaggtcgag
      301 gacccggtac ggggcgagtg ccggcgcggg gctcgtcccg gcctgcggtt tactctggct
      361 cttcgttggc aaggcttggc cctcgctcgc caccagccgg gagcggatgt gtagtcccga
      421 tcggcggctg actgtagcag gcgccaatcg cggttcgacc gaggaggtcc gtggatgagc
      481 gggttcgagc tgtccgaaga gcacgagcag cttcgccggg tggtgcggga ctttgccgag
      541 gccaggctgg ctcccaacgt cgggcggtgg gacgagcaac aggtcttccc tgtcgatgcg
      601 gtgctcgcca tgggggagct ggggctgttc ggcctggtgt tcccgccgga gtggggcggg
      661 tccggcggcg acttcacctc gctgtgcgtc gccgtcgagg agatcggccg ggtcgatcag
      721 tcgctgggca tcaccctctc agccggcgtc gggctcggcg ccaacccgat ctaccggttc
      781 ggcgacgacg agcaacgggc gaagtggctg ccggacctgg tggccggacg cgccctcggg
      841 gccttcgcgc tgaccgagcc ggacggcggc agcgacgccg gggcggctcg gtcgcgggcg
      901 cgccgcgacg gtgacgggtg ggtcctcgac gggtcgaagg ccttcatcac caactccggg
      961 actgcgatca cctcggtggt cacggtgacc gcccgcaccg atgtcggcat ctccgccttc
     1021 ctggtccccg ccgggacgcc ggggctcgtc gtcgagcctc cctaccgcaa gctcgggtgg
     1081 catgccagcg acacccacgg tctggtgctg gacgggtgcc ggctcccggc cgagagcctc
     1141 ctgggcgagc cgggcgccgg tctccacaac ctcctgtcga tcctcgacga cggccgcatc
     1201 gcgatctccg ccctcgcgct cggctgcgcc cgggcatgcc tcgagctgtc cgtcggctac
     1261 gccagggatc gggaggcctt cggtgggccg atcgggcggt tccaggccat cgcgttcgcc
     1321 ctgtccgacg tggacgtggc ggtggagaac gccaggaacc tcacgtacaa ggcggcctgg
     1381 ctgaaggacg ccggacgacc ggtcggtcgg gccgcggcca tggcgaagct ctactcgacg
     1441 gaggcggcgg tggccgcctc gcgggtggcg acgcaggtct tcggcggcgc cggcttcatg
     1501 gaggagaccc cggtggcccg gttctaccgg gacgcaaagg tcctggagat cggcgagggc
     1561 acgagcgaga tccaacggct ggtgatcgcc cgctcgctcg ggctgcccgt cggctgaggg
     1621 gaggcgtggt ggcgacgctc actaggttgt gccgggacgc caatctcagg aggcgcgcat
     1681 gcctgaagtg ctcggccacg tcgacgatgg atggggaaag gtggcggacg ccttccggtc
     1741 caacctggag gaccacgacc tcggggcggc attctgcgcc gaggtcggcg gccgccgggt
     1801 cgtcgacgtc tggggcggcg tggccgatgc ccgcaccggc cgcccttggc aggaggacac
     1861 catcgccgtg gtgttctcgg tcacgaaggg agcggtggcg atctgcgcca accgtttggt
     1921 cgagcgcggc gagctggacc tcgacgagcc ggtggcgacg tactggccgg agttcgcggc
     1981 ccacgggaag ggggcgatcc cggtgcggtg gctgcttacc caccaggctg gcctgccggt
     2041 ggtcgacgtc cccctgacgg tgcaggacgt ctgcgcgtgg tacccggtca tacgggcgct
     2101 ggaggcgcag cgaccgctgt gggagccggg ctcccggcac gcgtaccacg cccacacgtt
     2161 cgggttcctc gtcggcgagg tcgtccgtcg cgcccacggc cggaccgtcg gggccatgtt
     2221 cgccggggac gtggcgcgcc cgctcggcct gagcgcctgg atcgggctcc ctgagggcga
     2281 ggaagatcgg gtcgcgcacc tccgggccgc gccacctgcc ccgcccgaga tgg
//
LOCUS       DASWGG010000231         2874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3119677, whole genome shotgun sequence.
ACCESSION   DASWGG010000231 DASWGG010000000
VERSION     DASWGG010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3119677"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VF954_04190"
     CDS             <1..715
                     /locus_tag="VF954_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9530316.1"
                     /translation="ELKDQGFVVISVAMDTRGEESARPFIEAAHPSYPVLIDQEHRVS
                     ELYNMVNVPQAVWIDEEGDIVRPTEVAGSNDGFRPRTADLTPEQLAELRGTKSRYVEA
                     IKDWVAKGTASEFALSPDEVRARMRLPSEEVGLANAHFRLGLFLRTHGRAEEGDGHLA
                     EAKRLRPESWNFKRQAWNLEAPGDERSSGPDFWAAVAALGDDPYYEPIDMPGMPRPPG
                     WPGPRGQDGGREEGPTPGS"
     gene            complement(728..2113)
                     /gene="radA"
                     /locus_tag="VF954_04195"
     CDS             complement(728..2113)
                     /gene="radA"
                     /locus_tag="VF954_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX9530317.1"
                     /translation="MARAQTIHRCGECGGSTPRWAGRCPACGAWNALVEERAVARAPS
                     RPAARGVAFAAVPLTEIAPEGGAARSTGLPELDRVLGGGLVPGSVTLVGGEPGIGKST
                     LLLQVLAAMAGSGARGLLVSAEESPVQVQLRAERLDALHPSVWLLSETLLPTILGTAE
                     EIRPDVVVVDSIQTVSDPDLDSAPGSVAQVRECAHQLVGLAKQQAVTTLLVGHVTKDG
                     ALAGPRVLEHLVDTVLSFEGERHHALRLLRAVKHRFGATGELGLFEMAEQGLVAVPDA
                     SGLFLADRRQGTPGSVVLPAMEGQRPLLVEVQALVAGTTNPMPRRTASGLDGGRLHLV
                     LAVLERRARLRIPAADIYASVIGGVRVNEPAADLAMALALASAHTGVAVPHEVVACGE
                     IGLGGEVRQVAHLARRLAEAARLGFRTAVVPAASPDGPPGLELIRVNTLGEALASLEV
                     NPRRESLHPRR"
     gene            complement(2189..>2874)
                     /locus_tag="VF954_04200"
     CDS             complement(2189..>2874)
                     /locus_tag="VF954_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530318.1"
                     /translation="VHADGGGVLSATVTLDREAAQAVPDLGVQLQLADLRQAGWVVTG
                     PSQVAGGGFSVEAAKPFASPAEAMADASELSGPAGPVRNLVVRRHASLLSVSTAVSLD
                     ADLSARLEAFADDAFRARLGGHALGVDEAALAAQAGKPVDQLVSFRVEVRAPGAVPRA
                     VDVPLGATRRLAVASTVRRVRLALEAGAAVVASVIALVLAWARRRADRAHWTIGGGKG
                     RRDRWSVRR"
BASE COUNT          408 a         1090 c         1084 g          292 t
ORIGIN      
        1 ggagctgaag gaccagggct tcgtcgtgat cagcgtggcc atggacaccc gcggcgagga
       61 gtcggcccgc cccttcatcg aggccgccca cccgagctac ccggtcctga tcgaccagga
      121 gcaccgcgtc tcggagctgt acaacatggt caacgtgccc caggccgtgt ggatcgacga
      181 ggagggcgac atcgtgcgtc cgacggaggt ggcgggctcc aacgacggct tccggccgag
      241 gacggccgac ctcacccccg agcagctcgc cgagctccga ggcaccaaat cccgctacgt
      301 cgaggccatc aaggactggg tggccaaggg gacggcgagc gagttcgccc tgtcgcccga
      361 cgaggtgagg gcgcgcatgc gcctgccctc agaggaggtg gggctggcca acgcccactt
      421 ccgcctcggg ctgttcctgc gcacgcacgg gcgggcggag gagggcgacg ggcacctggc
      481 cgaggccaag cggctgcgcc ccgagagctg gaacttcaag cgccaggcgt ggaacctgga
      541 ggcgccgggg gacgagcgct cgagcgggcc cgacttctgg gccgcggtcg cggctctcgg
      601 cgacgacccc tactacgagc ccatcgacat gccgggcatg ccccggcctc cggggtggcc
      661 ggggccccgc gggcaggacg ggggccgcga ggaagggccg acccccggct cctgagccgg
      721 gccgcgctca gcgccggggg tggagcgact cccggcgagg gttcacctcc agggacgcga
      781 gcgcctcgcc cagggtgttg acccggatca gctcgagccc gggcgggcca tcgggggagg
      841 ccgccggcac gaccgcggtg cgaaacccga gccgggcggc ctcggccagg cgccgcgcga
      901 ggtgggcgac ctggcggacc tcgccgccca ggccgatctc gccgcacgcc accacctcgt
      961 gcgggacggc cacgcccgtg tgggccgacg ccagcgccag ggccatcgcc aggtcggcgg
     1021 ccggctcgtt gacccggacg cccccgatca ccgaggcgta gatgtcggcg gcggggatcc
     1081 gcagccgggc gcggcgctcg agcaccgcca gcacgagatg gagccggccg ccgtcgaggc
     1141 cggaggccgt ccgccggggc atggggttgg tggtcccggc gacgagggcc tggacctcta
     1201 cgagcagcgg gcgctggcct tccatggcgg gcagcacgac ggagccaggt gtgccctggc
     1261 ggcggtcggc gaggaacagc ccgctggcgt cgggcacggc caccaaaccc tgctcggcca
     1321 tctcgaacag acccagctcg ccggtcgccc cgaagcggtg cttgacggcc cgcagaagcc
     1381 gcagggcgtg gtggcgctcg ccttcgaagg acaggaccgt gtcgaccagg tgctccagca
     1441 cccggggccc cgccagggcg ccgtccttgg tgacgtggcc gacgaggagg gtcgtcaccg
     1501 cctgctgctt ggccaggccc accagctggt gggcgcactc ccgcacctgg gcgaccgacc
     1561 ccggggccga gtccaggtca gggtcgctga cggtctggat cgagtccacg accacgacgt
     1621 cgggccggat ctcctcggcg gtgcccagga tggtcggcag gagcgtctcg gacagcagcc
     1681 ataccgacgg gtgaagggcg tcgaggcgct cggcccgcag ctgcacctgc acgggcgact
     1741 cctcggccga gaccagcagg ccccgcgccc ccgagccggc catggccgcc agcacctgga
     1801 gcagaagcgt cgacttcccg atgccgggct cgccgccgac cagggtcacg gacccgggca
     1861 ccaggccgcc gccgaggacc cggtccagct cgggcaaccc cgtggaccgg gccgctcccc
     1921 cctccggtgc gatctccgta aggggcacgg cggcgaaggc cacgccgcgc gcggcggggc
     1981 gtgagggcgc ccgggcgacc gctcgctcct cgaccaaggc gttccacgcc ccgcacgccg
     2041 ggcatcggcc cgcccaccgc ggcgtggacc cgccacattc accgcaccgg tggatggtct
     2101 gcgcccgcgc catggagccg agcgtacgca gggccagtga caccgccgtg gaccctcggg
     2161 tggcttcgag gccgaggcgg gacggtggtc agcgccgcac cgaccagcgg tcgcggcggc
     2221 ccttgccccc gccgatggtc cagtgggccc ggtccgcccg ccggcgagcc cacgccagga
     2281 ccagggcgat gaccgacgcc acgaccgccg ccccggcctc gagcgccagc cgcacgcggc
     2341 gcacggtcga ggccaccgcc aggcggcggg tggcaccgag gggcacgtcc accgccctcg
     2401 gcacagcacc gggcgcccgc acctccaccc ggaaggacac gagctggtcg acgggcttgc
     2461 ccgcctgggc cgccagcgcg gcctcgtcca cccccagggc gtggcccccg agccgggccc
     2521 gaaaggcgtc gtcggcgaac gcctccagcc gagccgagag gtcggcatcc agcgacacgg
     2581 cggtggacac cgacagcagc gaggcgtggc ggcggacgac gaggttgcgc accggcccgg
     2641 cggggcccga gagctcggac gcgtccgcca tggcctcggc cggcgaggcg aacggcttgg
     2701 cggcctctac cgagaaaccg ccgcctgcca cctgcgaggg gccggtcacg acccagccgg
     2761 cctggcgcag gtcggccagc tggagctgca ccccgaggtc gggcacggcc tgggcggcct
     2821 cccggtcgag cgtcaccgtc gcggagagca ctcccccgcc gtcggcgtgc acga
//
LOCUS       DASWGG010000232         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2360103, whole genome shotgun sequence.
ACCESSION   DASWGG010000232 DASWGG010000000
VERSION     DASWGG010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2360103"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..1766
                     /locus_tag="VF954_04205"
     CDS             357..1766
                     /locus_tag="VF954_04205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX9530319.1"
                     /translation="MLSSILGSFVGIGASRASLPTTLTVKVIVQQWDNSDTTVRALVT
                     QVGGLVTQDLPVVDGFAATLPSAALATVAASPAVRVISPDSMVLPQDLPSTLGPPSPD
                     LAVIDDAPAQRAGADGHGVTVALVDTGVANVADFARRIVPVTGLNGQASTCVNFSSDP
                     DCSDNYGHGTFVAGLIAGSGASSNGKFTGVAPAANLLSVKLAGRDGGSTVSKVLAAIG
                     WVLVNKDAYNIRVLNLSLRVDSTLSYRLDPVNLAVERAWAAGLVVIVSAGNQGSSAAT
                     IAKPADDPWVISVGSVDDQGTANLKDDTPSSFSSRGPTVADGLAKPDLAVSGRHLVSL
                     RSPGSAADLSYPYFYDANYRYGSGTSFSAGIVSGAAAALLSSQPSASNDRVKYQLVGA
                     GRPISGAGPMDVGSGVPDMVAALNAGAGVANADNFHPVFWPTNPGVPADFVGPIVWVG
                     SNWQGSNWQGSNWQSAYWQ"
     gene            1892..>2917
                     /locus_tag="VF954_04210"
     CDS             1892..>2917
                     /locus_tag="VF954_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530320.1"
                     /translation="MTALTAGLAMAGAVAAFAGRPGHQWKAAGPWWTALPLLAALVMA
                     GRLQVDFSYRGHLEALDLFETALVPTVLLFAGPVAVALAVVAKAVSQRLLHVSGRKAA
                     FNVASWAASTGVASWVYLSLGGAGLGATNRVPALVAGMVAAMLVNHLALATVMGLVSE
                     TGLRGALAGLSSVIVPGWLVGGAINLACGVLFAIVAWRAPALVALILVPLVMLNWAER
                     AYAEARTDRARLQALQRATHDLAAPVDPLEGVPDFLASLLDCFQAAGVAVVVDGGAGF
                     HCGEADTEDTCRLIARRVDRSAAAHRVRANDGSAAGQVLAATGRRSALVAPLWRDRDV
                     TGLLVSLD"
BASE COUNT          368 a         1049 c         1046 g          454 t
ORIGIN      
        1 ctggtgccca cccgggtggt ctacgcggat gtcggcgagg ggggctgcga ctaccggctg
       61 gccttctgcg acctggacga gaccgacgcc gcccgtctcg ccgagctcgt cggggtgacc
      121 gcagccacct gaccgccgcc ccacctgccg ggccgcctgg gccgagcggg actggtccga
      181 tcgggtgatc ccgaaatagt tgggacgggc catggtcgtg aagggccgct cccctcatac
      241 ttcatggaga aagttcactc gtcgcgctca ggagggtacg ccccgtggcg gtccgtcgct
      301 ctctcgccaa agcccgcagc accagagccc gcagcaccca ggttcttgtc ggtgtggtgc
      361 tgtcgagcat tctggggagc ttcgtgggga tcggggccag ccgggccagc ctgccgacga
      421 ccctcaccgt caaggtgatc gtgcagcaat gggacaactc cgacacgacc gtccgggccc
      481 tcgtcaccca ggtcggcggg ctggtgaccc aggatcttcc ggtggtcgac ggcttcgccg
      541 ccaccctgcc ctccgccgcc ctggcgacgg tggccgccag ccccgcggtt cgggtcatct
      601 cccccgacag catggtcctg ccccaggacc tgccctccac gctcggcccg ccgtcgcccg
      661 acctggcggt gatcgacgac gcgcctgccc agagggctgg cgccgacggc cacggtgtga
      721 ccgtcgccct ggtcgacacc ggggtggcca acgtggccga cttcgcccgg cgcatcgtgc
      781 ctgtcaccgg cctcaacggc caggcgagca cctgcgtcaa cttctcgtcc gatccggatt
      841 gcagcgacaa ctatggccat ggcacgttcg tcgccggcct gatcgccggc agcggggcct
      901 cgtcgaacgg caagttcacc ggtgtcgccc ccgccgccaa cctgctgtcc gtgaagctgg
      961 ccggccggga cggcgggtcc acggtgagca aggtgctggc ggccatcggc tgggtgctcg
     1021 tcaacaagga cgcctacaac atccgcgtcc tcaacctgtc gctgcgggtc gactccaccc
     1081 tgagctaccg cctcgacccg gtgaacctcg ccgtcgagcg ggcctgggcg gcgggcctgg
     1141 tggtcatcgt gtccgctggc aatcagggct cgagcgccgc caccatcgcc aagcccgccg
     1201 acgacccgtg ggtcatcagc gtgggctccg tcgacgacca gggcacggcc aacctcaagg
     1261 acgacacgcc gtcgagcttc tcgagccggg ggccgacggt cgcagacggg ctggccaagc
     1321 ccgacctggc cgtgtccggg cgccatctgg tgtcgttgcg gtcacccggc agcgcggccg
     1381 acctcagcta cccgtacttc tacgacgcca actaccgcta cgggtcgggc acctcgttct
     1441 cggccgggat cgtcagcggg gcggccgccg ccctgctctc gtcccaaccg tcggcgagca
     1501 acgaccgggt gaagtatcag ctggtcgggg ccggccgtcc catctcgggt gccgggccga
     1561 tggatgtcgg ctccggcgtg cccgacatgg tcgccgccct caacgccggc gcgggcgtgg
     1621 ccaacgccga caacttccat cccgtcttct ggccgaccaa tccgggcgtg ccggccgact
     1681 tcgtcgggcc catcgtgtgg gtgggctcca actggcaggg ctcgaactgg cagggctcga
     1741 actggcagag tgcgtactgg cagtgacgcg ccagttgatc ttgcactgat gccacaccga
     1801 tgccgcccct gaccacgagg ggatcgaggg gacggagata gtgggagagc acagcaagcg
     1861 aagcgccgcc cgccggccgc cagcatggtc cgtgaccgcc ttgaccgccg ggctggcgat
     1921 ggccggtgcg gtggccgctt tcgccgggcg gcccgggcac cagtggaagg ccgccggacc
     1981 ctggtggacg gcgctgccgc tgctggcggc cctggtgatg gcgggtcgcc tccaggtcga
     2041 cttcagctac cgcggccacc tcgaggcgct ggacttgttc gagacggccc tggtgccgac
     2101 tgtgctgctc ttcgccgggc cggttgccgt ggcgctggcg gtggtcgcca aggccgtctc
     2161 gcagcggttg ctgcacgtgt cgggcaggaa ggcggcgttc aacgtggcca gctgggccgc
     2221 gtccacgggg gtggcaagct gggtctacct gtcgctggga ggcgctgggc tcggggcgac
     2281 caaccgggtt ccggctctgg tcgccggcat ggtggcggcc atgttggtca accatctggc
     2341 cctggcgacg gtgatgggcc tggtgtccga gaccgggctg cgcggggccc tggccggtct
     2401 gtcctcggtg atcgtgccgg gctggctcgt cggcggcgcc atcaacctgg cgtgcggggt
     2461 gctcttcgcc atcgtggcgt ggcgcgcccc ggccctggtg gcgttgatcc tggtgcccct
     2521 ggtgatgctc aactgggcgg agagggcgta cgccgaggcc cgcacggacc gggcgcgcct
     2581 tcaggccttg cagcgcgcca cccacgacct ggccgctccc gtcgacccgc tcgaaggcgt
     2641 gcccgacttc ctcgcctccc tgctcgactg cttccaggcc gcgggcgtag cggtggtcgt
     2701 cgacggcggc gcgggcttcc attgcggcga ggcggacacg gaggacacct gccgcttgat
     2761 cgcgcgacgg gtggaccggt cggccgcggc ccaccgggtc cgggccaacg acggcagcgc
     2821 cgcgggccag gtgctcgcag ccacgggccg gcgcagcgcc ctggtggcgc ccctgtggcg
     2881 ggaccgcgac gtcaccggcc tgctcgtgtc gttggac
//
LOCUS       DASWGG010000233         3932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3121648, whole genome shotgun sequence.
ACCESSION   DASWGG010000233 DASWGG010000000
VERSION     DASWGG010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3121648"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1256
                     /locus_tag="VF954_04215"
     CDS             138..1256
                     /locus_tag="VF954_04215"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEX9530321.1"
                     /translation="MTRPTRTTGCFAVIGGGGTGGHVQPALAVGRALVDAGHDRVSIH
                     FVGSRRGLERRLVPEAGFAITLLPGRGIARHLTVDNVGAAWGVAAAVVQAVGVMRRLR
                     PAVALSVGGYAGLPVALAAVVCRVPLVLAEQNAVPGAANRLTGRFARAAAVSFAGTAL
                     PRAVVTGNPLRPEILEVDTSPEGRAKARVDLGLPEGRTVVAAFGGSLGSGTINAAARG
                     LAELWRDRDDLAIYHVVGRRDWGSAAGPGPGPLLYRTVEYEDRMPLLLAAADVAVCRS
                     GGSVAELAAVEVPSVLVPLPGAPGDHQRANAMAFVDRGAAVLVPDAECTAPRLAEVLG
                     ALIGESGRLNAMSSAAAGLARPDAAARVVDLLERHARV"
     gene            1243..2655
                     /gene="murC"
                     /locus_tag="VF954_04220"
     CDS             1243..2655
                     /gene="murC"
                     /locus_tag="VF954_04220"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX9530322.1"
                     /translation="MPAFDLARPRRAHLVGIGGAGMRAYASVLVSMGHTVTGSDLRDS
                     AGLDRLRGLGVTVMIGHDPANVGPAEAVATSTAIPASNVEIVEAHRRGIPVLRRAELL
                     AAICGTRRTVAVSGTHGKTTTTSMLALILRQDGMRPSFIVGGDVNELGTGAAWDHGDW
                     LVVEADESDGTFLEVGAEAVVVTNVEADHLDHYGSLEGLTAAFASFVDGAAGTRVLNA
                     DDPIAATIAARYGATTFGVGETADYQLVGVELARSRARFSIAHEGHAIGPIELPLPGM
                     YNAMNAIAAVAAGIELGASAASAVDALARFGGVARRFQFRGEWNGVSFVDDYAHLPGE
                     VASVLAAAAGGGWARVVAVFQPHRYSRTVALWQDFGASFGGAGLVVVTDVYASDEAPR
                     PGVTGQLIAQAIAGADPDQAVVYQPSRPSLVDFLRHELRPGDLCLTLGAGDITLLADE
                     VRAPDGSSLGRAGGSPAEGA"
     gene            2658..3665
                     /gene="murB"
                     /locus_tag="VF954_04225"
     CDS             2658..3665
                     /gene="murB"
                     /locus_tag="VF954_04225"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX9530323.1"
                     /translation="MGAGAEGGVQEVARRLGPRARRQVPLGPRTTYRVGGAARLFLEA
                     ESEEDLELVRRAVQGTGMPVLVVGQGSNLLVSDAGFPGLALVLGEAFTTIQLPEGGGA
                     RAGGAVALPVLARRTAAAGLTGLEWAVGVPGSVGGGVRMNAGGHGSDMAAVVKAVRLV
                     DLRAPDAAAAGLEWSIHELAPGYRSSAVRPWHVVCSAELALRPGDPARGLAEIAEIVS
                     WRRANQPGGQNAGSVFSNPPGDSAGRLVDQAGLRGHRRGSAHVSEKHANFIQADPGGS
                     ADDVWALILEVQRLVERRLGVRLEPELRLVGFPEGESPSVNGGSASTSSEGSGSGSGG
                     P"
     gene            3662..>3932
                     /locus_tag="VF954_04230"
     CDS             3662..>3932
                     /locus_tag="VF954_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530324.1"
                     /translation="MRGPVVTDPAAPAAPVAPVRVPIDPRIRARRIEVRRGEGRRRLR
                     LLTASLGACALLGAGVGALHSPLFAASTVAVAGNRQTSRASIMAAT"
BASE COUNT          433 a         1426 c         1527 g          546 t
ORIGIN      
        1 gggctgctcc cggacaccgg tgtgcccctg cccttcgtgt ccttcggcgg atcgtcgctc
       61 ctgatcgcca tgggagccgt cgggatgctg ctcaacgtgg ccaggcagga gcaccgcagc
      121 gggtcgccgg cgcggccatg acgcgcccga cccgcacgac gggctgcttc gcggtcatcg
      181 gagggggagg aacgggaggc cacgtgcagc ccgccctggc cgtgggtcgg gccctggtcg
      241 acgccggcca cgaccgcgtg tccatccact tcgtcgggtc caggcgaggc ctcgagcgcc
      301 ggctggtgcc cgaggcgggc ttcgccatca ccctgctgcc cggccggggc atcgctcggc
      361 atctcacggt cgacaacgtc ggcgcggcct ggggcgtggc cgccgccgtc gtgcaggccg
      421 tcggggtgat gcgccgcctc cgtcccgcgg tcgccctctc cgtcgggggc tacgcagggc
      481 tgccggtcgc cctggcggcc gtcgtgtgcc gggtgcccct cgtgctcgcc gagcagaacg
      541 ccgtgcccgg cgcggccaac cggctcaccg gtcgcttcgc ccgggccgcg gccgtgagct
      601 tcgcgggcac ggccctgccc cgggccgtgg tcaccggcaa ccccctgaga ccggagatcc
      661 tggaggtgga cacctcgccg gaagggcgag ccaaggctcg agtcgacctc ggcttgccgg
      721 aggggagaac cgtcgtcgcg gccttcggcg gctccctcgg ctcgggcacc atcaacgccg
      781 cggcgcgggg cctggccgag ctgtggcgtg accgggacga cctcgccatc taccacgtgg
      841 tgggtcgccg cgactggggc tcggcggcgg ggccgggccc gggcccgctc ctctaccgca
      901 cggtcgagta cgaggaccgc atgccgctgt tgctggcggc cgcggacgtc gccgtgtgcc
      961 gctcgggcgg ctcggtcgcc gagctcgctg ccgtggaggt cccttcggtg ctggtcccgt
     1021 tgccgggtgc acccggggat caccaacggg ccaatgccat ggccttcgtg gatcgagggg
     1081 cggcggtgct cgtgccggac gccgagtgca ccgcgccccg gctggcggag gtgctcggcg
     1141 cgctcatcgg tgagagcggc cgcctgaacg cgatgtcgag cgcggcggcc gggctggccc
     1201 ggccggacgc ggcggctcgc gtcgtggacc tcctggagcg ccatgcccgc gtttgacctc
     1261 gcccgtcccc ggcgcgccca tctggtgggg atcggtggcg cgggcatgcg ggcctacgcg
     1321 tccgtcctcg tcagcatggg ccacaccgtc actgggagcg acctgcgcga ctcggccggt
     1381 ctcgaccgcc tgcgcggcct cggggtcacg gtgatgatcg gtcacgaccc cgccaacgtc
     1441 ggcccggcgg aagcggtcgc cacctccacc gccatccccg cgtcgaacgt cgagatcgtc
     1501 gaggcgcacc gacggggcat cccggtgctc cgtcgagccg agctgctcgc cgccatctgc
     1561 ggcacgcgcc ggaccgtcgc cgtctccggg acccacggca agacgacgac aacgtcgatg
     1621 ctggccctca tcctccgcca ggacgggatg cggccctcgt tcatcgtcgg cggcgacgtg
     1681 aacgagctcg gcacgggcgc ggcctgggac cacggcgact ggctggtggt cgaggccgac
     1741 gagagcgacg gcaccttcct cgaggtgggt gccgaggcgg tggtagtgac caacgtggag
     1801 gccgaccacc tcgaccacta cggctcgctc gaggggttga cggcggcgtt cgcctccttc
     1861 gtcgacggcg cagccgggac ccgggtcctc aacgcggacg acccgatcgc ggcgacgatc
     1921 gcggcccggt acggcgccac gacgttcggg gtgggcgaga cggccgacta ccagctggtc
     1981 ggtgtggagc tggcgcgcag ccgggcccgg ttctcgatcg cccacgaggg tcatgccatc
     2041 ggcccgatcg agctgccgct gcccggcatg tacaacgcca tgaacgcgat cgcggcggtg
     2101 gccgcgggga tcgagctcgg cgccagcgcg gcgtcggctg tcgatgccct ggcgcgcttc
     2161 gggggcgtgg cccggcggtt ccagttccgc ggcgagtgga acggcgtgag cttcgtcgac
     2221 gactacgccc acctaccggg cgaggtggcc agcgtgctgg cggcggcggc ggggggtgga
     2281 tgggcgcggg tcgtggccgt gttccaacct caccggtact cgcggacggt cgccctctgg
     2341 caggacttcg gcgcgtcgtt cgggggggcg gggctggtgg tcgtcaccga cgtgtacgcc
     2401 tcggacgagg cgccccggcc gggcgtgacc gggcagctga tcgcgcaggc gatcgccggc
     2461 gccgacccgg accaggccgt cgtgtaccag ccgtcgcggc cctccctggt cgacttcctc
     2521 cgccacgagc tgcgccccgg cgatctgtgc ctgaccctcg gcgcgggcga catcaccctg
     2581 ttggcggacg aggtgcgcgc tccggacgga tcgtcattgg ggcgcgccgg cgggtcgccg
     2641 gcggaaggcg cctgaccgtg ggcgccggcg cagagggcgg cgtgcaggag gtggcccggc
     2701 ggctcgggcc ccgggctcgc cgccaggtgc ccctcggccc gcgcacgacc taccgagtcg
     2761 ggggagcggc ccggctcttc ctcgaggcgg agtcggagga ggacctcgag ctcgtgcgca
     2821 gggcggtcca gggcactggc atgcccgtgc tcgtcgtcgg ccagggttcc aacctgctgg
     2881 tgtcggacgc gggcttcccg ggcctggctc tggtcctcgg cgaggccttc acgaccatcc
     2941 agctccccga gggcggcggg gcccgggcag gtggtgcggt ggcgctgccc gtgctggccc
     3001 gtcgcaccgc cgccgccggc ctcaccgggc tggagtgggc cgtcggggtc ccgggatcgg
     3061 tgggcggcgg cgtgcgcatg aacgccggcg ggcacggctc cgacatggcc gcggtcgtga
     3121 aggcggtgcg cctggtcgat ctccgggcgc cggacgcggc tgccgccggc ctggagtggt
     3181 cgatccacga gctggcgcct ggctaccgca gctcggccgt gcggccctgg cacgtggtgt
     3241 gctcggccga gctcgcgctc cgccccgggg acccggcgcg gggcctggcc gagatcgccg
     3301 agatcgtgag ctggcggcgg gcgaaccagc ccggcgggca gaacgccgga tcggtgttca
     3361 gcaaccctcc cggtgactcg gccggccggc tcgtcgacca ggccggcctg cggggccacc
     3421 ggcgaggatc cgcgcacgtc tccgaaaagc atgccaattt catacaggcc gatcccggag
     3481 gctcggcgga cgacgtgtgg gccctcatcc tggaggtgca gcgcttggtg gagcgacgac
     3541 tgggggtacg gctcgagccc gagctccggt tggtcgggtt cccggagggg gagtccccct
     3601 cggtgaacgg cggatcagcc tcaacctcga gcgaaggctc aggctcaggc tccggcgggc
     3661 catgaggggt cccgtcgtga ccgatccggc ggcgccggcg gccccggtgg cgcccgtgcg
     3721 ggtgccgatc gacccgcgca tccgcgcccg gcgcatcgag gtccgccgtg gcgagggccg
     3781 ccgccggctc cgcctgctga cggcgtcgct gggcgcctgc gccctgctcg gcgcgggggt
     3841 gggtgcactg cactcaccac tgttcgcggc gtccaccgtg gccgtcgccg gcaaccgtca
     3901 gacctctcgg gccagcatca tggccgcgac cg
//
LOCUS       DASWGG010000234         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_427662, whole genome shotgun sequence.
ACCESSION   DASWGG010000234 DASWGG010000000
VERSION     DASWGG010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427662"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..>1544
                     /locus_tag="VF954_04235"
     CDS             274..>1544
                     /locus_tag="VF954_04235"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9530325.1"
                     /translation="MPVHPPTVGQLREIAESFGLVLSDEDVASFQGLILPLLASYTEV
                     EGMAGVTPLAPKYRRGSGFRPDPSDNPYNAWYWRCEIPGAAGGPLAGKRVAIKDSVCV
                     AGVPMMNGSATLEGYVPEVDATIVGKAVCEDLCFSGGSHTAATGPVLNPRDPTRSAGG
                     SSSGSAALLAAGEIDLAIGGDQGGSIRVPAAWTGVVGHKPTYGLVPYTGIFPMELTLD
                     HTGPMAATVADVALYLEVLAGADGLDPRQPAVVPVERYSEGLDGDLSGLRVGVLAEGF
                     GWEGLSEAAVDDAVVEAARRLEKAGAAVGPVSVPMHRQGLAVWMTIAMEGVTDLMVDG
                     NGMGTNWKGHYTTSLLDAYARGRQARVNDLADTVKLVMLLGRYLRSTSGGRHYAMGQN
                     VAPRLRAAYDAALDEVDVLVMPTVPMLPTPIP"
BASE COUNT          208 a          568 c          559 g          209 t
ORIGIN      
        1 ggggcaacaa atagccgcag aaatcactgc cgaaaaacca tcgccgggcc gggtcgtggg
       61 gcggaaccca actccgtccc cgacctggcc cgcgccagcc tcgtcgggcc tcgagccaat
      121 gtcgccgccg gggcgccggc gacaccggcg cacctgaccg ggggacaagc cttccctctc
      181 cccccggcag gtgcgccgcg atgatcgggc ccttcgagcc agtccgtccc ggcgccgggt
      241 agcgtcagcg ccgtcgccga gggggaggtg cccatgcccg tccatccgcc caccgtcggg
      301 cagctacggg agatcgccga atcgttcggg ctcgtgctct ccgacgagga tgtcgcttcc
      361 ttccagggtc tgatccttcc gctgctggcc tcgtacaccg aggtggaggg gatggcgggg
      421 gtgacgccgc tggcgccgaa gtaccgccgc ggctccggat tccggcccga cccgtccgac
      481 aacccgtaca acgcctggta ctggcggtgc gagatccccg gtgcggccgg cgggccgctg
      541 gccggcaagc gggtggccat caaggacagc gtgtgcgtgg ccggcgtccc gatgatgaat
      601 gggtcggcga cgctggaggg ctacgtcccc gaggtcgacg ccaccatcgt cggcaaggcc
      661 gtctgcgagg acctgtgctt ctccggcggc agccacaccg ccgccaccgg gcccgtcctc
      721 aacccccgcg acccgacccg gtccgcgggc ggctcctcct ccggctcggc cgccctgctg
      781 gcagcgggcg agatcgacct ggccatcggc ggcgaccagg gcggctcgat ccgcgtgccg
      841 gcggcctgga cgggcgtcgt cggccacaag ccgacctacg gcctcgtccc ctacaccggc
      901 atcttcccga tggagctgac gctcgaccac accgggccga tggcggccac cgtggccgac
      961 gtcgccctgt acctggaggt gctggccggc gccgacgggc tcgacccccg ccagccggcg
     1021 gtggtgccgg tcgagcggta ctccgaaggg ctcgacggcg acctgtctgg cctgcgggtc
     1081 ggcgtgctgg ccgagggctt cgggtgggag ggcctgtcgg aggcggcggt cgacgacgcg
     1141 gtggtcgagg ccgcccggcg gctggagaag gccggggcgg cggtgggccc ggtgtcggtg
     1201 ccgatgcacc gtcagggcct tgccgtgtgg atgaccatcg ccatggaagg cgtcaccgac
     1261 ctcatggtcg acggcaacgg catgggcacg aactggaagg gccactacac cacgagcctg
     1321 ctcgacgcct acgcccgggg tcggcaggcc cgggtcaacg acctggccga cacggtgaag
     1381 ctggtcatgt tgctcggccg gtacctgcgg tccacctccg gcgggcgcca ctacgccatg
     1441 gggcagaacg tggccccccg cctccgggcc gcctacgacg ccgccctcga cgaggtcgac
     1501 gtgctggtga tgcccacggt gccgatgctg ccgacaccga tccc
//
LOCUS       DASWGG010000235         3520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_427690, whole genome shotgun sequence.
ACCESSION   DASWGG010000235 DASWGG010000000
VERSION     DASWGG010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427690"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..839
                     /locus_tag="VF954_04240"
     CDS             75..839
                     /locus_tag="VF954_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530326.1"
                     /translation="MTLGRPGAAGALAAAGYLAAVALTGMLASRLVRPLFEGFQPAPA
                     YRWVRPPPPFATGNQAPLPGDGSVALRDGAATGSAAGGVVTPDGQVTVSLGPGAFGRH
                     PGDTAATITITPVDPGALAPLPPGLRADGNAYRIDMAYSPSRAGVGALAAPGDVTLAV
                     PVPAKGLARTGPGRPWEALSGLRVAGGTGETAALPGPGVYVAVAAATPSTEIVTPGAG
                     RPVLGTAAVAAATAALAALMVLAPTALLRHRKPGPS"
     gene            950..1933
                     /locus_tag="VF954_04245"
     CDS             950..1933
                     /locus_tag="VF954_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530327.1"
                     /translation="MAATDYRSWLLEISPGTPGLEVTLLDLGRRIRVSNRTGSTFVVV
                     GYSGEPYLRIGPHGVDENLRSPATYQNRVTPAGTTPTTLPAIADAHAEPAWRRVSGGT
                     SAVWRDRRTRWEGPAPQEVRSSPGRAVPVSDWFIELDRGDGTAVVLQGRITFVPAPSA
                     WPWLVLAATLAALAVAAGLARRWGALLSCGLAVLVATDVARNVGGAAARGGSVGAQVA
                     RLLGTSMVPMVLWVVAALAMRPLERSKEAGLIGAGIAALGIGVYSGVSDIPNLFRSQE
                     PVAFAPALARLAVAVSLGLGIGITAAVFLRERRFDRGEGRRSVTRGAHVTP"
     gene            1987..2058
                     /locus_tag="VF954_04250"
     tRNA            1987..2058
                     /locus_tag="VF954_04250"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2019..2021,aa:Pro,seq:tgg)
     gene            2087..2157
                     /locus_tag="VF954_04255"
     tRNA            2087..2157
                     /locus_tag="VF954_04255"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2119..2121,aa:Gly,seq:tcc)
     gene            complement(2337..2537)
                     /locus_tag="VF954_04260"
     CDS             complement(2337..2537)
                     /locus_tag="VF954_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530328.1"
                     /translation="MYHEGRFGRVEDDQFEQVAGLVRVCGEVTRRVVIELHPADQMLE
                     CVGDVLVGHPMPSGSSMDLHTQ"
     gene            2645..2875
                     /locus_tag="VF954_04265"
     CDS             2645..2875
                     /locus_tag="VF954_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX9530329.1"
                     /translation="MIQELADEAEGGYDSAKLQGKPRGRGRPPLGDAAKAVESVRLDP
                     DLQDQTSQRAAEEGVTVSEIIRRALRQYLKSA"
     gene            complement(3091..3213)
                     /locus_tag="VF954_04270"
     CDS             complement(3091..3213)
                     /locus_tag="VF954_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530330.1"
                     /translation="MSDAHEANDEAVTVLDVDDQAWLDDQLREYAELLAYLREY"
BASE COUNT          484 a         1269 c         1237 g          530 t
ORIGIN      
        1 gccgagttcg tggcgaccga ccacctgccc ttcgccaacc ggttgcgggc ggtggtcatc
       61 ttcacggtgc aggggtgacc ctcgggcggc ccggcgccgc cggagcgctg gcggccgccg
      121 gctatctcgc cgccgtcgcc ctcactggga tgctggcgtc ccggctggtg cggccgctgt
      181 tcgaggggtt ccagcccgcg cccgcctacc ggtgggtgcg gccgccgccg ccgttcgcca
      241 ccgggaacca ggcaccgctg ccgggcgacg gctcggtcgc cctgagggac ggcgccgcca
      301 ccggctcggc ggcaggcggc gtggtcacgc ccgacggcca ggtgaccgtg agcctcggcc
      361 cgggtgcctt cgggcgccac ccgggcgaca cggccgccac catcaccatc acgccggtcg
      421 atccgggcgc cctggcgccg ctccccccgg gcctgcgcgc cgacgggaac gcctaccgga
      481 tcgacatggc ctactccccc tcccgcgccg gtgtcggcgc gctcgccgcc ccgggcgacg
      541 tcacgctggc ggtgcccgtc ccggccaagg gcctggcgcg caccggcccg ggccggccgt
      601 gggaggcgct gtccgggctg cgcgtggccg ggggcaccgg ggagaccgcc gccctgccgg
      661 gaccgggggt ctacgtggcg gtggcggcgg cgactccgtc gaccgagatc gtgacgcccg
      721 gtgccggtcg gccggtgctc ggcaccgccg cggtcgccgc cgccaccgcg gccctggccg
      781 ccctcatggt cctcgcgccc accgccctcc ttcgacaccg gaagcccggc ccgtcatgaa
      841 ccgccggcgc gctctgacgg gggggcggcg ggcggcggta gcggcggtcg ccgttctccc
      901 catcctggtg gcaaccgcgg ggccggcatc ggcgcacacc gtcgccgggg tggccgccac
      961 cgactaccgc agctggttgc tcgagatcag ccccggcacg cccggtctcg aggtgaccct
     1021 gctcgatctc ggccggcgca tccgggtcag caatcgcacc gggtcaacct tcgtcgtggt
     1081 cgggtactcg ggcgagccgt acctgcgcat cggcccgcac ggggtcgacg agaacctccg
     1141 gtcacccgcc acctaccaaa accgggtgac acccgccggc acgaccccga ccacacttcc
     1201 ggcgatcgcc gatgcccacg ccgagcccgc gtggcggcgg gtgtcgggcg ggacgagcgc
     1261 ggtgtggcgg gaccggcgga cccggtggga ggggccggcg ccccaggagg tgcgctcctc
     1321 gcccgggcga gccgtgcccg tgtcggactg gttcatcgag ctcgaccggg gcgacggcac
     1381 ggcggtcgtg ttgcaggggc ggatcacttt cgtgccggcg ccgagcgcgt ggccgtggct
     1441 ggtgctcgcg gccaccctgg ccgcgctcgc cgtggccgcc gggctggccc gccggtgggg
     1501 cgccctgctc tcgtgcgggc tggccgtgct cgtggcgacg gacgtcgccc gcaacgtcgg
     1561 cggggccgcg gcgaggggtg gctcggtcgg tgcccaggtc gcccggctgc tggggacgag
     1621 catggtcccg atggtgctct gggtcgtcgc cgcgctggcc atgaggccgc tggagcgctc
     1681 gaaggaggcc gggctgatag gtgcgggcat cgccgccctc gggatcgggg tctacagcgg
     1741 cgtctcggac atccccaacc tcttccggtc tcaggagccg gtcgcgttcg cccccgcgct
     1801 ggcgaggctg gcggtcgccg tctcgctggg cctcgggatc gggatcaccg ccgcggtctt
     1861 cctgcgggag cggcgcttcg accgcgggga gggccggagg tcggtgaccc gcggggcgca
     1921 cgtcacgccg tgagttccca gttgggggcc accaccggga acgaccacgt acgctggcgg
     1981 gcaccacggg gagtggcgca gtggtagcgc tccagctttg ggtgctggtg gtcgagggtt
     2041 caagtccctc ctccccgact cactaactac tacggtgcac cacctcgcgg gcgtagttca
     2101 gaggcagaac ctgagccttc caagctcatg acgccggttc gattccggtc gcccgctctt
     2161 cgacaccgct cttcgacacc gctcttcgac accggtccaa caacccgttt gcgatcccgg
     2221 acgtgtggcg acggttgaaa ctggtccttc ggtctgcgca cccgagatgc tccggcactt
     2281 gtcgcatccc cgtgaggggg tcgggtggtt gtcttctacc atttctcgta ttacgcttat
     2341 tgcgtgtgga gatccatcga gctgcccgac ggcatggggt gtccgacgag aacatcgccc
     2401 acgcattcga gcatctggtc tgctgggtgc agctcgatga cgaccctccg cgttacctcg
     2461 ccgcagaccc ggaccaggcc ggcaacctgc tcgaactggt catcctcgac gcgcccgaaa
     2521 cgcccctcgt gatacacgcc atgtccctca gacggaccac ccaggacgag ttgttcggaa
     2581 ccgaggaccc attacgcaac gcatctacgg acacaccaag agcggcaagc cgatcgacga
     2641 caaggtgatc caagagctcg ctgacgaggc cgaaggtggc tacgactcgg ccaagctgca
     2701 aggcaagccc cgcggcagag gacgaccccc cctcggtgac gccgccaagg ctgtcgaatc
     2761 agtacgtctg gatcccgacc tacaggacca gacgtctcag cgggccgccg aggaaggcgt
     2821 caccgtttca gagatcatcc gccgagcgct ccgccagtac ctaaagagcg cctgaccaag
     2881 cggtatcgcc gaggaccagc gataaccggt ccggtcgcta cgaccgcgac tcgaaggtcg
     2941 cctgccgcca cccccggtgg ggacggtacc tatcggcggg cgactggtca gcgcgctgaa
     3001 ctcgctcaag gtcgtcgggc tcgtcgatcc actcatcgtt gtcacggacg gcgcggttga
     3061 tcgccaccat gaggtcgagg ggcgggagct tcaatactct cgaaggtagg cgagcagctc
     3121 tgcatactct cgaagctggt cgtcgagcca tgcctggtcg tcgacgtcga ggaccgtgac
     3181 ggcctcatca ttcgcctcgt gcgcgtcgct catgaggtcc agggtaccca cgccccaggt
     3241 cccgtctgcc ttcaatcgcc acagcgagcc atgcggacga cttggcctct gacaaaggtg
     3301 agacggctag ctcgacgaca cgacgtcatc ctgcacattc cgcatgcgtt catcggcgac
     3361 tgggtagcga tggagatatg gccaacagcg acgcgggccg gcggtggtgg cttcagacat
     3421 atggcgttcc gccgtggcgc ctcaggcgac tcagggatcg gtggcctgac tgggaccacc
     3481 tcaccccaat cgagcgggcc cagcgagaac gggtggcgtt
//
LOCUS       DASWGG010000236         5229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1195156, whole genome shotgun sequence.
ACCESSION   DASWGG010000236 DASWGG010000000
VERSION     DASWGG010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195156"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VF954_04275"
     CDS             <1..710
                     /locus_tag="VF954_04275"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9530331.1"
                     /translation="PRRGRRWHEGALRRAVARADVLLTAAPETAQALRAAGAARVEVV
                     AHGADHLPPADHEGAHDALARAGVTGPYLLAVGTLEPRKNMARLFSAHALARARLPEP
                     WPLVVVGPNGWGDRVRPVAGVVLLGRVPDAVLAGIYAGARCLAYVPLLEGFGLPAVEA
                     MAACTPVVASPMPSTGGAALEVDPTDTEAMADALVTAAGDDCERRRLVAGGKARAGEL
                     TWAAAARRHVAIWRSLR"
     gene            707..1909
                     /locus_tag="VF954_04280"
     CDS             707..1909
                     /locus_tag="VF954_04280"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9530332.1"
                     /translation="MTPAPLRLALDVTAVPDNPTGAGRYTVNLAAALGRHDEVALQLV
                     TRAHDERWSALVPGADLLAAAPRRRPLRLAWEQAAFPRLLRGIDVDLLHSPHYTMPEA
                     AALRRVVTIHDLTFFEHPEWHEKFKVPFFRRAIRVAARHADGLVCVSQDTADRLRELC
                     RPAGPVHVVPHGVDLSRFRPDEPESGADDAVLAGLGVRTPYVTFVGTLEPRKDVPTLI
                     RAFDKMAAAHAGLSLVIAGGSGWGGNAVEAAAASARNRERIVQPGYVPDDAVPALLRR
                     AAAVAYPSLEEGFGLPALEALACGSPLVTTTGSAMEEIAAGSALLVAPGDADGLAGAL
                     DMLARGDAGMPARRRRGLAIAARHTWEASAEGHVAAYRDVIGRPARGPVHRPAKVSRR
                     VRSRLGGH"
     gene            1936..2829
                     /locus_tag="VF954_04285"
     CDS             1936..2829
                     /locus_tag="VF954_04285"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX9530333.1"
                     /translation="MRVYLTGGHGFVAQWLRRHLEAEGDQVVGSGAEVDVTDPAAIKE
                     AVVAARPDAIYHLAAFTHVGRSWDDPVQVFTVNATGTVNVLQAARACPGPPRVLLVGS
                     AEAYGPVKADQLPLTEDSPLMPVTPYALGKVAAEFAGIQAHLGYQLPVIRVRPFNHVG
                     PGQSPDFVLSALAQRLVEAERTGAPSITVGNLAARRDFTDVRDVVRAYRLLVCGGQAG
                     AVYNVCSGRDLSIAELADELVRLSGRGVALVPDPELTRPVDIPVLRGDPRRLHLATGW
                     EPTVPLEQTLLDVLEAWRVAP"
     gene            complement(2855..3214)
                     /locus_tag="VF954_04290"
     CDS             complement(2855..3214)
                     /locus_tag="VF954_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530334.1"
                     /translation="MPDVGKVAKDTAYLIIGLGVLAFQRAQVQRQELRKRLEGQRKDM
                     EGQLEEIRALLHKVARDIDGRVEPVIEDLESRLDVVEERLPTQARQLVHQAREQAKEA
                     RAQVRSQLTGNGTSRAA"
     gene            3340..>5229
                     /locus_tag="VF954_04295"
     CDS             3340..>5229
                     /locus_tag="VF954_04295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9530335.1"
                     /translation="MDPHAPPVVAVVVTCDPGPWLEEALAALAAQDYPNLSVLVVDAG
                     SADDPTRRVAAILPGAFVRRLGANPGYGPASNEALTVVEGASHYLFCHDDAAPDPDAV
                     RVMVEEAFRSNAGVVSPKYVTWDDPRRLVQVGLAADKSGAPAVLVERGELDQEQHDGV
                     TDVFWAPGGFTLVRADLFAVLRGFDPAISLYGEDLDLSWRAQLAGARVVVAPAARVRH
                     LEADASGIRVAIPASAGLAPGDRSGPERLRRRHQLRVVLKNYSFWRLLVIVPQVALLA
                     LAELVVAVANRRWSEGRALAEAWSWNGRRLGDLRRARREVRRVRTLSDRELRRLQVRG
                     SARLRAFVRSQVSEGGYRDRRAARVAARGSLRQILTVWAVVIFVVGVGSRQLLSRLPA
                     IGELQPLPPAGELFTRYLSGWRPTGLGSAAPAPVALALLGLGGAALGGAVGLLRTMLV
                     LAALPAGAVGAYRLGRALPGLRAPLVLLVAFLAVPLPYDDLARGRVQGLVAWAAAPWV
                     LGALVRAGGLASAEAGGRGGTHRRSLARASITLGVALAAVASVSPALVLATPALGLGL
                     WSGGLMAGRAGSRPGSGPDRPVRLLGVAIGGAAVAVGLLFPWSLDFARPGSQVAALTG
                     VAPAAA"
BASE COUNT          530 a         1996 c         1954 g          749 t
ORIGIN      
        1 tcccccgccg cggccgtcgc tggcacgagg gcgccctgcg ccgggccgtg gcccgggccg
       61 acgtgctgct caccgcggcc ccggagacgg cgcaggccct ccgggccgcc ggtgcggctc
      121 gcgtggaggt ggtcgcccac ggcgccgacc acctgccgcc ggctgatcac gagggtgcac
      181 acgacgccct ggcccgggcc ggtgtcaccg ggccgtacct cctcgccgtc gggaccctcg
      241 agccccgcaa gaacatggcc cgcctgttct cggcccacgc gctcgcccgc gcccggcttc
      301 ccgagccgtg gccgctggtc gtggtcggcc ccaacgggtg gggggaccgg gtcaggccgg
      361 tcgcgggcgt ggtgctgctc ggccgggttc cagacgccgt gctcgccggc atctacgccg
      421 gggcgcggtg cctcgcctac gtcccgctcc tggagggctt cggcctgccc gccgtcgaag
      481 ccatggccgc atgcacgccg gtcgtggcca gcccgatgcc gagcaccggc ggggcggccc
      541 tggaggtcga cccgaccgat accgaggcca tggccgacgc gctggtcacc gccgccggcg
      601 acgactgtga gcgccgccgc ctggttgccg gcggcaaggc ccgcgccggg gagctgacgt
      661 gggcggccgc ggctcgccgc cacgtcgcca tctggaggag cctgcggtga cccctgctcc
      721 gctgcgcctc gccctcgacg tcaccgcggt gcccgacaac cccaccgggg cggggcgcta
      781 caccgtcaac ctggcggccg ccctgggtcg ccacgacgag gtcgccttgc agctggtgac
      841 gcgcgcccac gacgagcgct ggtcggctct cgtgcccggg gccgaccttc tcgcagccgc
      901 gccgcgccgc cggccactgc gcctggcctg ggagcaggcc gcctttcccc gactgctccg
      961 gggcatcgac gtggacctgc tccacagccc ccactacacg atgccggagg cggctgcgct
     1021 gcggcgggtg gtgacgatcc acgacctcac cttcttcgag caccccgagt ggcacgagaa
     1081 gttcaaggtg ccgttcttcc ggcgcgccat ccgggtcgcg gcccgccacg ccgacgggct
     1141 ggtctgtgtc agccaggaca cggccgaccg cctccgggag ctgtgccggc cggccgggcc
     1201 cgtccatgtc gtgccccacg gcgtcgacct gagccggttc cgtcccgacg agcccgagtc
     1261 gggcgccgac gacgccgtgc tcgccggcct cggcgtccgg accccgtacg tcaccttcgt
     1321 cgggacgctc gagccccgca aggacgtgcc cacgctgatc cgggcgttcg acaagatggc
     1381 ggcggcccac gccggcctct cgctcgtgat cgcgggcggc tcgggatggg gcggcaacgc
     1441 cgtggaggcg gctgccgcgt ccgcccggaa ccgcgagcgc atcgtccagc ccggctacgt
     1501 gcccgacgat gccgtgcccg ccctcctgcg gcgagccgcg gcggtggcct acccgtcgct
     1561 ggaggagggc ttcgggctgc cggccctcga ggccctggcc tgcggcagcc cgctcgtcac
     1621 cacgacgggc tcggccatgg aggagatcgc ggccggctcc gccctgctgg tggcgccggg
     1681 ggacgccgac ggcctggccg gcgcgctcga catgctggcc cggggcgacg ctggcatgcc
     1741 cgcccgccgc cggcggggcc tggccatcgc ggcgcgccac acctgggagg ccagcgccga
     1801 ggggcacgtg gccgcctacc gcgacgtgat cggccgcccg gcgcgtggcc cggtccaccg
     1861 gccggcgaag gtctcccgcc gggtgcgcag ccggctcggg ggccattgag cgcgcgttca
     1921 ttagggtggg gcgcaatgag ggtctacctg accgggggac acggcttcgt ggcccagtgg
     1981 ttgcgccgcc acctggaggc ggagggcgac caggtggtcg ggtcgggcgc cgaggtcgat
     2041 gtcaccgacc ccgccgccat caaggaggcg gtcgtcgcgg cccggcccga cgctatctac
     2101 cacctggccg ccttcaccca cgtgggacgg tcctgggacg acccggtcca ggtgttcacc
     2161 gtgaacgcca ccggcacggt gaacgtcctc caggccgccc gggcctgccc cggcccgccg
     2221 cgcgtcctgc tggtgggctc ggccgaggcc tacggccccg tcaaggccga ccagctgccc
     2281 ctgaccgagg actctcccct gatgccggtc acgccgtacg cccttggcaa ggtggcggcc
     2341 gagttcgccg ggatccaggc ccacctgggc taccagctgc ccgtgatccg ggtcaggccc
     2401 ttcaaccacg tcggcccggg ccagtccccc gacttcgtgc tctccgctct ggcccagcgc
     2461 ctggtcgagg ccgaacgcac gggcgccccg tccatcaccg tcggcaacct ggcggcccgc
     2521 cgggacttca cagacgtccg ggacgtcgtg cgggcctacc gtctcctggt ctgcggcggg
     2581 caggccggcg ccgtgtacaa cgtgtgctcc ggccgggacc tctccatcgc cgagctggcc
     2641 gacgagctgg tccgtctcag cgggaggggg gtggcgctgg tgcccgatcc ggagctgacc
     2701 cggcccgtgg acatcccggt cctgcggggc gacccccgcc ggctgcacct ggcgaccggc
     2761 tgggagccca ccgtgcccct agagcagacg ctcctcgacg tgctggaggc gtggcgggtg
     2821 gctccctgac cggcgcggtc gccgggcgcg gtcgctaggc cgcccgggac gtgccgttcc
     2881 cggtgagctg gctgcggacc tgggcccggg cctccttggc ctgctcgcga gcctggtgca
     2941 cgagctggcg ggcctgggtg gggaggcgct cctcgaccac gtcgaggcgg gactcgaggt
     3001 cctcgatcac gggctcgacc cgcccatcga tgtcgcgggc gaccttgtgg agaagggccc
     3061 gtatctcctc cagctggccc tccatgtcct tccgctggcc ctcgagccgc ttgcgcagct
     3121 cctggcgctg gacctgggcc cgctggaagg ccagcacccc gagaccgatg atcaggtaag
     3181 ccgtgtcctt ggcgaccttg cctacgtccg gcatggctcc tcctagcttg tcgtctttcg
     3241 ccgtcttgct agctaccata ggtcttggca taaacaattg caagcgaggc tgggctggtt
     3301 cctctgcccg ggccgccggc ctatagcctc acgagccgaa tggatcccca cgccccgccc
     3361 gtggtggccg tcgtggtgac atgcgacccg ggtccctggc tcgaggaggc gctcgccgcc
     3421 ctggccgccc aggactaccc caacctctcc gtcctggtcg tcgacgccgg cagcgccgac
     3481 gacccgaccc ggcgggtggc ggcgatcctg cccggggcgt tcgtacggag gctgggcgcg
     3541 aaccccgggt acggaccggc cagcaacgag gcgctgaccg tcgtggaggg ggcgtcgcac
     3601 tacctcttct gccacgacga cgccgccccc gatccggatg cggtccgggt catggtcgag
     3661 gaggcgttcc gttccaacgc cggcgtggtc tctcccaagt acgtcacctg ggacgaccca
     3721 cgccggctgg tccaggtcgg cctggccgcg gacaagagtg gcgcccccgc tgtcctggtg
     3781 gagcggggcg agctggacca ggaacagcac gacggcgtga ccgacgtgtt ctgggcgccg
     3841 gggggcttca cgttggtgcg cgccgacctg ttcgccgtgc tgcgcggctt cgaccccgcc
     3901 atcagcctct acggcgagga cctcgacctg agctggcggg cgcagctggc cggggcgcgg
     3961 gtcgtcgtgg cgccggcggc gcgggtgcgc cacctcgagg ccgacgcgtc cgggatccgc
     4021 gtcgccatcc cggcctcggc cggcttggcg ccgggcgacc ggtcggggcc cgagcggctc
     4081 cgccgccggc accagctccg ggtcgtgctc aagaactact cgttctggcg cctgctcgtg
     4141 atagtgccgc aggtcgccct gctcgccctg gccgagctgg tggtggccgt ggcgaaccgg
     4201 cgctggagcg agggccgggc cctggcggag gcgtggagct ggaacggccg ccggctgggc
     4261 gacctgcggc gtgcccgccg ggaggtcaga agggttcgta cgctgagcga ccgcgagctg
     4321 cgccggctcc aggtgcgggg cagcgcccgc ttgcgggcct tcgtgcgcag ccaggtgtcc
     4381 gaaggcggct atcgagaccg ccgggccgcc cgcgtggccg cccggggatc gctgcgccag
     4441 atcttgaccg tgtgggccgt tgtgatcttc gtggtggggg tggggagccg ccagctgctc
     4501 tcccgcctgc cggcgatcgg cgagcttcag ccgctgcccc cggcgggcga gctgttcacc
     4561 cgctacctct ccggttggcg cccgaccggc ctgggctcgg ccgccccggc gcccgtggcg
     4621 ctggccctgc tcggcctggg cggcgccgcg ctgggcggtg ctgtcgggct gttgcggacc
     4681 atgctggtgc tggctgcgct cccggccggc gccgtcggcg cctaccgcct cggtcgggcc
     4741 ctgcccggat tgcgggctcc gctcgtcctg ctcgtcgcct tcctcgccgt gccgctgccc
     4801 tacgacgacc tggcccgggg ccgggtccag gggctggtcg cctgggcggc ggcgccgtgg
     4861 gtcctcggcg ccctcgtgcg ggcgggcggt ctggcctctg ccgaggcggg tgggcggggc
     4921 gggacccatc gccgctcgct ggcccgggcg agcatcacgc tgggcgtggc cctggctgcc
     4981 gtggcctctg tctcgccggc cctggtcctg gccacgcccg cgctcggtct cgggctgtgg
     5041 tcgggcgggc tgatggcggg gcgcgccggc tcccggcccg ggagcggacc tgaccggccg
     5101 gtgcgtctcc tcggcgtggc catcggcggt gcggcggtgg ccgtgggact gctcttcccg
     5161 tggtcgctcg acttcgcccg gccgggaagc caggtggcgg ccttgaccgg cgtggcgccc
     5221 gccgccgcc
//
LOCUS       DASWGG010000237         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_867592, whole genome shotgun sequence.
ACCESSION   DASWGG010000237 DASWGG010000000
VERSION     DASWGG010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867592"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..2322
                     /locus_tag="VF954_04300"
     CDS             124..2322
                     /locus_tag="VF954_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HEX9530336.1"
                     /translation="MSLARRRPGALTAELVREPGGFGLGQVPARLKPDAITSMVCGFC
                     STGCSLDVHVRDGEAVNLSPTTEYPVNLGMACPKGWEALAPLGAADRATTPLWRDGRG
                     RLQPVGWDVAMATFVERFRAILDSHGPESVAFLSTGQIPTEEMALLGALAKFGMGLVH
                     GDGNTRQCMATSVVAYKEAFGFDAPPYTYADLEQSDVIVLVGSNLCIAHPILWERIAR
                     NPHRPEIVVVDPRATETAMAATQHHAVRPKTDLAFLYSVAHVLVRRGWIDRDFVAGHT
                     RGFDELAAHVAGFPPERVAPICGVRPEAIEQLASTVHRGQRVSFWWTMGVNQSHEGTR
                     TAQAIIDLALMTGNIGRPGTGANSVTGQCNAMGSRLFSNTTSLFGGRDFTDASARAEV
                     AALLGMDVGRIPARPSLAYDEIVDGVAAGRIKGLWVVATNPAHSWIDQGHLRHALARL
                     EFLVVQDMYTTSDTARLAHLVLPAAGWGEKEGTFINSERRVGVVKRVARAPGQALADF
                     SIFLMIAEAWGCAPLLAEWTSPEAVWGIITRLSAGRPCDMTGITCYRMIDELGGIQWP
                     FPAGSPLPAVGSERRLFEDGRFHHGDGRARFVFEQPRPVAEPASALYPLVLLTGRGSS
                     SQWHTQTRTAKSAVLRSLSAADPYVEVAPRDAEARRLVTGDWAVVVSAHGSMRARVVV
                     TPTVGEGQVFVPMHYAEANRLTNRSFDPYSRQPSYKSGAVQLTRAGRRPR"
BASE COUNT          315 a          922 c          884 g          375 t
ORIGIN      
        1 gtcctcgctg ggcggtcacc cctcgttggt gtgggtgatg ctggccaccc tcggcctggt
       61 cggtgtggtc gcgggcgagc tggccgagcg gtcgctcttt ttcaccaccg ccagcccgcc
      121 ccgatgagcc tggcgcggcg ccgcccgggc gcgctcaccg ccgagctggt gcgcgagccg
      181 ggcggcttcg ggttgggcca ggtgccggcg cggctcaagc ccgacgccat cacctcgatg
      241 gtgtgcgggt tctgctcgac cggctgctcg ctggatgtgc acgtgcgcga cggcgaggcc
      301 gtgaacctgt cgccgaccac cgagtacccg gtcaacctcg gcatggcctg ccccaagggt
      361 tgggaggcgc tggcgccgct cggggcggcc gaccgggcga cgacgccgct gtggcgcgac
      421 ggcaggggtc gtctccagcc cgtcggctgg gacgtggcga tggccacctt cgtcgagcgc
      481 ttccgggcca tcctcgacag ccacggcccg gagtcggtcg ccttcctctc gactggccag
      541 atcccgaccg aggagatggc tctgctcggc gcgctcgcca agttcggcat gggtctcgtc
      601 cacggcgacg gcaacacccg ccagtgcatg gccacgtcgg tggtcgccta caaggaggcc
      661 ttcggcttcg acgccccgcc gtacacctac gccgacctcg agcagtccga tgtcatcgtc
      721 ctggtcggct ccaacctctg catcgcccat cccatcctgt gggagcgcat cgcccgcaac
      781 ccgcaccggc cggagatcgt ggtcgtcgat ccgcgcgcga ccgagacggc gatggccgcc
      841 acgcagcacc acgcggtgcg ccccaagacc gatctggcgt tcctctacag cgtggcccac
      901 gtcctcgtcc gacgagggtg gatcgatcgc gacttcgtgg ccggccacac ccgcggcttc
      961 gacgagctgg cggcccacgt cgccggcttc cccccggagc gcgtcgctcc gatctgcggg
     1021 gtccggcccg aggccatcga gcagctggcc tccaccgtcc accggggcca gcgggtctcg
     1081 ttctggtgga cgatgggtgt gaaccagagc cacgagggca cccggaccgc tcaggccatc
     1141 atcgacctgg cgctgatgac gggcaacatc gggcggcccg gcaccggcgc caactcggtg
     1201 acgggccagt gcaacgccat ggggtcccgg ctgttcagca acaccacgag cctgttcggc
     1261 gggcgggact tcaccgatgc gagcgcccgg gccgaggtgg ccgcccttct cgggatggac
     1321 gtgggccgca tccccgcccg gcccagcctg gcctacgacg agatcgtgga cggtgtggcc
     1381 gctggccgga tcaagggcct gtgggtcgtc gccaccaacc cggcccactc gtggatcgac
     1441 cagggccacc tgcggcacgc cctggcccgg ctcgagttcc tggtcgtgca ggacatgtac
     1501 accacgagcg ataccgcccg gctggcccat ctcgtgctgc ctgcggccgg atggggcgag
     1561 aaggagggca ccttcataaa ctccgagcgc cgggtcgggg tcgtcaagcg ggtggcgcgc
     1621 gcgcccgggc aggcgctggc ggacttctcc atcttcctga tgatcgccga ggcatgggga
     1681 tgcgctccgc tccttgccga gtggacctcc cccgaggcgg tgtggggcat catcacccgg
     1741 ctctcggcgg ggcggccctg cgacatgacc gggatcacct gctatcgcat gatcgacgag
     1801 ctgggtggca tccagtggcc cttcccggcc ggctcgcccc tgccggcggt cgggagcgag
     1861 cggcggctgt tcgaggacgg gcgcttccac catggcgacg ggcgggcccg gttcgtgttc
     1921 gagcagcccc gtcccgtggc cgagccggca tcggccctct atccccttgt gctcctgacc
     1981 ggccgcggca gctcgagcca gtggcacacc cagacgcgta cggcgaagtc ggcggtgctg
     2041 cggtccctca gcgccgccga cccctatgtc gaggtggctc cacgcgacgc cgaggcgcgc
     2101 cgcctggtga ccggcgactg ggcggtcgtc gtgtccgccc acgggtcgat gcgcgcccgc
     2161 gtggtggtca ccccgacggt gggtgaaggc caggtgttcg tgccgatgca ctatgccgag
     2221 gccaaccgcc tgaccaaccg gtcgttcgat ccctactccc gccagccctc ctacaagagc
     2281 ggcgcggtcc agctcacccg ggccgggcgg cggccccggt gaagctgcgg tcgcggcgct
     2341 cgaccggcgc gaggcaaggt gttctcgcaa gcgcggccca cgtggaggcc cggcgggcgg
     2401 ccgccacccg cctggccggg ggtacggacg tggcggcccg catcgcctcg gcccctcctg
     2461 gctatctgct ggtccatggc ccgagcgaca tcgcgc
//
LOCUS       DASWGG010000238         5041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2793012, whole genome shotgun sequence.
ACCESSION   DASWGG010000238 DASWGG010000000
VERSION     DASWGG010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793012"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..630)
                     /locus_tag="VF954_04305"
     CDS             complement(64..630)
                     /locus_tag="VF954_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530337.1"
                     /translation="MKICPACGLPAPSTTATCAFCEASYPHPAPLSFRLEEHDGAYRW
                     TGEDAVVAVASWAAGMCHVRQPGRDAVSLTLVPMAGDGAVRVAMVDAGARLVSTLQPS
                     AGPATLSTVCDRYEHPRLVVKGDGPTGIHIVDMSGDVVALASRDRGAPGLALDVLVLR
                     PRGDGETVLFGVVLATELLRTGELRPVG"
     gene            complement(635..1711)
                     /locus_tag="VF954_04310"
     CDS             complement(635..1711)
                     /locus_tag="VF954_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX9530338.1"
                     /translation="MPDRSSRSRTGDHDLDELLAAILDRAGATANRDLLLDILADSVR
                     LAGEDVERLDLKIMSAALNEMAEAFRVFAPYRDRPKVTMFGSARTLPDDPLYAQAREL
                     AEAMARSGWMVVTGAGPGIMAAGLEGAGRDMAFGINIRLPHEQGANEFIDNDPKLVEM
                     KYFFTRKLMLMKESDAFVVLPGGFGTLDESFELLTLLQTGKAVPAPLVFLEVPGGTFW
                     QSWTSFLRHEVEPRGLISSEDHHLWMVTDDVGEAAGEVLGFYRNYHSIRWVGRTLVIR
                     MRRGPDDQELAALNATFGELCLKGGIQRAEPFPAEVAGQDHLELDRIALRFDYVHYGR
                     LRQLIDVLNTYPANTSSPDSPQSR"
     gene            complement(1790..4066)
                     /gene="cofH"
                     /locus_tag="VF954_04315"
     CDS             complement(1790..4066)
                     /gene="cofH"
                     /locus_tag="VF954_04315"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEX9530339.1"
                     /translation="MHEELLARPLAELLARAASRRDEITGTRVTYSPKVFIPLTHLCR
                     DRCHYCTFAQPRARVQAPYLVPDEVLAIARSGAAAGCHEALFTLGERPELRYPAAAGW
                     LAEAGYATTVDYLVAMAALVRDETGLLPHGNAGALSADELAQLRPVTASQGMMLESLN
                     AGLACHAGSPDKTPGRRLATLEAAGQLAIPFTTGILCGIGESRADRLAALEAIAASHR
                     RHGHVQEVIVQNFLPKQGTAMHLTPPCPDEELLWSAAVARLVLPDDVHLQAPPNLSDD
                     FGVLLGAGIDDWGGVSPVTIDHVNPERPWPALERLAAVTEGHGFTLAPRLTLYPRYVG
                     APARWLDPAMRFAVMDRSDAEGLGREPGERWFSGADVDPPPLLPVQVPVGRARAGGAV
                     GEVLAGVLLGQEVGVDEIVTLFSARGPEVAAVAAVADDLRRSAVGEVVTWVATRNINY
                     TNICTFKCRFCAFSKGPLSLSLRGDPYLLELDEVTRRVVEAVEAGCTEVCLQGGIHPN
                     FDGDYYLDVIRAVRAGSPDIHIHGFTALEVTEGARRLGEPLAAYLGRLMAAGLKTLPG
                     TAAEILDDEIRAELCPDKITTEEWLEAHRCAHGLGLRSNVTIMFGSIERPRHWARHLV
                     RTRDLQKETGGFTELVPLPFVHMASPIYLQRRSRRGPTFRETVLMHAVGRIAYRGWID
                     NVQASWVKIGPHGARQLLQAGVNDLGGTLMDENISRAAGANHGQMMDEAGFRAVVDPL
                     GRPLEQRTTVYGRVAARA"
     gene            complement(4078..4755)
                     /gene="pgl"
                     /locus_tag="VF954_04320"
     CDS             complement(4078..4755)
                     /gene="pgl"
                     /locus_tag="VF954_04320"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX9530340.1"
                     /translation="MHGELLVVDDVPAEFSRRVAAAFHARPDEEFCLTLSGGDTARRC
                     YEHLADAAASDIDWWRVSVYWGDERCVPPDSPDSNQRLGREALLERVGAANAIYPMSC
                     EEGADAYHLRVAEVGKFDLVHLGLGPDGHTASLFPSSAGLDADPGRLVVVNRDPSGRN
                     PHERMTLTLSAIARSRLVVFTVAGADKQAAMQAVADGADVPAARVRADRVLWLVDPEA
                     APRESLT"
     gene            complement(4784..>5041)
                     /locus_tag="VF954_04325"
     CDS             complement(4784..>5041)
                     /locus_tag="VF954_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530341.1"
                     /translation="KLVPDDHVRITLTTGGDASARYLVERRGGEHVVRARISTPDGEQ
                     QSQLLALPDDSVPWSLGTALTRLRRDPVYEHALLGALTLSG"
BASE COUNT          701 a         1722 c         1951 g          667 t
ORIGIN      
        1 cgaccgggac tgacctaccg ggactgacct accgggactg accgaccggg accggtggcc
       61 ggctcagccg acgggccgca gctcgccggt tcgcagcagc tccgtggcca ggaccacgcc
      121 gaagaggacg gtctcgccgt cccccctcgg gcgcagcacg agcacgtcga gcgccagccc
      181 gggcgcgccg cggtcccggc tggcgagggc cacgacgtct cccgacatgt cgacgatgtg
      241 gatgccggtg gggccgtcgc ccttgaccac gaggcgggga tgctcgtagc ggtcacagac
      301 cgtgctgagc gtggcaggac cggcggaggg ctgcagggtg gacacgagcc gggcgccggc
      361 atcgaccatg gcgacgcgca cggcgccgtc gccggccatc ggcaccaggg tgagcgagac
      421 cgcgtcccgg ccgggctggc gcacgtggca catgcccgcc gcccacgagg ccaccgccac
      481 cacggcgtcc tcgccggtcc agcggtaggc gccatcgtgc tcctcgagcc ggaacgaaag
      541 gggcgctggg tgcgggtagc tcgcttcgca gaatgcgcac gtggcggtcg tagagggcgc
      601 gggaaggccg cacgccgggc agatcttcac cctgttacct cgactgcggg gagtcgggac
      661 ttgatgtatt ggcggggtag gtgttgagca cgtcgatgag ctggcgcagg cgcccgtagt
      721 gcacgtagtc gaagcgcagg gcgatgcgat cgagctccag gtggtcctgg cccgccacct
      781 cggcggggaa gggctcggcg cgctggatgc cgcctttgag gcaaagctcg ccgaaggtgg
      841 cgttgagcgc ggccagctcc tggtcgtccg gcccgcgacg catgcggatg acgagggtcc
      901 ggcccaccca gcggatggag tggtagttgc gatagaagcc gagcacctcg ccggcggcct
      961 cgccaacgtc gtcggtgacc atccacaggt ggtggtcctc ggacgagatc aggccgcgag
     1021 gctcgacctc gtggcgcagg aacgacgtcc acgactgcca gaacgtccct cccggcacct
     1081 ccaggaacac caggggggcg gggacggcct tgcccgtctg gagaagggtc agcagctcga
     1141 aggactcgtc gagggtgccg aacccgccgg gcagcaccac gaaggcgtcg gactccttca
     1201 tcagcatcag ctttcgggtg aagaagtact tcatctccac cagcttcggg tcgttgtcga
     1261 tgaactcgtt ggccccctgc tcgtgcggca ggcggatgtt gattccgaag gccatgtccc
     1321 gcccggcgcc ctccagcccg gcggccatga tgccgggccc ggccccggtc acgaccatcc
     1381 acccggaccg cgccatggcc tcggccagct cccgggcctg ggcgtagagg gggtcgtccg
     1441 ggagggtgcg cgccgagccg aacatggtga ccttgggccg gtcgcggtag ggggcgaaga
     1501 cccggaaggc ctcggccatc tcgttgaggg cggccgacat gatcttgagg tcgaggcgct
     1561 cgacgtcctc gccggcgagg cgcacgctgt cggccaggat gtcgagcagc aggtcccggt
     1621 tggcggtggc gccggcccgg tccaggatgg cggcgaggag ctcgtcgaga tcgtggtcgc
     1681 cggtgcggga gcggctggag cggtcgggca tggtgggctc aggctaacga ccggctggag
     1741 ctacatcggg agaaagggca cccggtgcgc cggagcgccg tgccgccggt caggcccgag
     1801 ccgccacccg gccgtagacg gtcgtgcgct gctcgagcgg gcgtccgagc gggtccacga
     1861 cggcgcggaa gccggcctcg tccatcatct ggccgtggtt ggcgccggcg gcccgggaga
     1921 tgttctcgtc catgagggtg ccgccgaggt cgttgacgcc ggcctggagc agctggcggg
     1981 cgccgtgggg gccgatcttc acccatgatg cctgcacgtt gtcgatccat ccccggtagg
     2041 cgatgcggcc cactgcgtgc atgagcacgg tctcccggaa ggtgggcccc cggcggctcc
     2101 tgcgctggag gtagatgggc gaggccatgt ggacgaacgg caacggcacg agctcggtga
     2161 agccgccggt ctccttctgg aggtcgcgcg tccggaccag gtggcgggcc cagtgccgcg
     2221 gccgctcgat cgagccgaac atgatcgtga cgttggaccg cagccccagc ccgtgggcgc
     2281 accggtgggc ctccaaccac tcctcggtgg tgatcttgtc cgggcacagc tcggcccgga
     2341 tctcgtcgtc gaggatctcc gcggccgtgc cggggagggt cttgagcccg gccgccatca
     2401 gccggccgag ataggcggcg aggggctcac cgaggcggcg ggcgccctcg gtgacctcca
     2461 gggcggtgaa gccgtggatg tggatgtccg gggagccggc ccgcacggcc cggatcacgt
     2521 cgaggtagta gtcgccgtcg aagttggggt ggatgccacc ctggaggcac acctccgtgc
     2581 accccgcctc caccgcctcc accacccgcc gggtgacctc gtcgagctcc aggaggtacg
     2641 ggtcgccgcg cagcgacagc gagagggggc ctttggagaa ggcgcagaag cggcacttga
     2701 aggtgcagat gttggtgtag ttgatgttgc gggtcgccac ccaggtgacc acctcgccga
     2761 ccgccgagcg ccggaggtcg tctgcgactg ccgccaccgc cgccacctcg gggccgcggg
     2821 ccgagaacag cgtcacgatc tcgtccaccc cgacctcctg gccgagcagg acgccggcca
     2881 gcacctcgcc cacggcgccg ccggcccggg ctctgcctac ggggacctgg accgggagca
     2941 ggggcggggg gtcgacgtcg gcgccggaga accaccgctc gccgggctcc cggcccaggc
     3001 cctcggcgtc ggagcggtcc atcaccgcga agcgcatggc cgggtccagc caacgcgccg
     3061 gcgcccccac gtagcgcggg tacagcgtta ggcggggcgc cagggtgaag ccgtggccct
     3121 cggtcaccgc cgccaggcgc tccagggccg gccagggccg ctcggggttc acgtggtcga
     3181 tggtgaccgg cgagacgccg ccccagtcgt cgatgccggc gccgaggagg acgccgaagt
     3241 cgtccgacag gttgggcggg gcctggaggt ggacgtcgtc ggggagcacc agccgggcca
     3301 cggccgccga ccacaggagc tcctcatcgg ggcagggcgg cgtcaggtgc atcgccgtgc
     3361 cctgcttcgg caggaagttc tggacgatca cctcctggac gtggccgtgg cgccggtggc
     3421 tggccgcgat ggcctcgagg gcggccaggc ggtccgcccg gctctcgccg atgccgcaca
     3481 ggatcccggt cgtgaacggg atggcgagct gcccggcggc ctccagggtg gccaggcgcc
     3541 ggcccggggt cttgtcgggc gagcccgcgt ggcaggccag gccggcgttg agcgactcga
     3601 gcatcatgcc ctggctggcc gtgaccgggc gcagctgggc cagctcgtcg gccgaaagcg
     3661 ccccggcgtt gccgtggggg agcagcccgg tctcgtcgcg caccagggca gccatggcga
     3721 ccaggtagtc caccgtcgtg gcgtatcccg cctcggccag ccacccggcc gcggccgggt
     3781 accgcagctc gggccgctcg cccagcgtga agagggcctc gtggcatccg gcggcggcgc
     3841 cggaccgggc gatggcgagc acctcgtccg ggaccaggta gggcgcctgg acccgggcgc
     3901 gcggctgggc gaaggtgcag tagtggcagc ggtcccggca gaggtgggtg agggggatga
     3961 acaccttcgg ggagtaggtg acccgcgtgc cggtgatctc gtcgcgccgg gacgccgccc
     4021 gggcgagcag ctcggcgagc ggccgcgcca gcagttcctc atgcatagcc gcttacctta
     4081 ggtcagtgac tcgcggggcg cagcctcggg gtccaccagc cacagcaccc ggtccgcccg
     4141 gacgcgggcc gcgggcacgt cggcgccgtc ggcgacggcc tgcatggccg cctgcttgtc
     4201 cgcgccggcc accgtgaaga cgaccaggcg ggagcgggcg atggcggaca aggtgagggt
     4261 catgcgctcg tgggggttgc gcccggaggg gtcgcggttg accacgacca ggcggccggg
     4321 gtcggcatcg agcccggcgg agctggggaa gagcgaggcc gtgtgaccgt cgggccccag
     4381 ccccaggtgc accaggtcga actttcccac ctccgccacc cgcaggtggt aggcgtcggc
     4441 gccctcctcg cagctcatgg ggtagatggc gttggccgcc ccgacccgct cgagcagggc
     4501 ctcgcgcccc aggcgctggt tggagtccgg gctgtccggc gggacgcatc gctcgtcgcc
     4561 ccagtacacg ctgaccctcc accagtcgat gtcggacgcg gcggcgtcgg cgaggtgctc
     4621 gtagcagcgc cgggccgtgt cgccgcccga gagcgtcagg cagaactcct cgtcggggcg
     4681 ggcgtggaac gcggcggcga cgcgccggga gaactccgct ggcacgtcgt cgacgacgag
     4741 cagttcgccg tgcatccggc gcacgctaat aggtccgggc ggctcagccc gagagcgtca
     4801 gcgcgccgag aagggcgtgc tcgtagaccg ggtcgcggcg caggcgggtg agggcggtgc
     4861 cgagggacca gggaacggag tcgtcgggca gggcgaggag ctgcgactgc tgctcgccgt
     4921 cgggcgtcga gatccgggcc cgcacgacgt gctcgccgcc ccggcgctcc acgaggtagc
     4981 gcgccgacgc gtcgccgccg gtggtgagcg tgatcctcac gtggtcgtcg ggtacgagct
     5041 t
//
LOCUS       DASWGG010000239         2705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_869524, whole genome shotgun sequence.
ACCESSION   DASWGG010000239 DASWGG010000000
VERSION     DASWGG010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869524"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VF954_04330"
     CDS             complement(<1..481)
                     /locus_tag="VF954_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX9530342.1"
                     /translation="MPTFVRCEVLAIGTELLLGQIVDTNSAWIGEQLALSGIDSHYQT
                     KVGDNQGRIVAALRVALGRSDAVICCGGLGPTQDDITREAIASLLGVALERDQSLVGA
                     IEALFMARGRSMPANNLRQADVPVGAIPIPQTQGTAPGLICQVGASGPRPGKVIYAVP
                     "
     gene            complement(459..1031)
                     /locus_tag="VF954_04335"
     CDS             complement(459..1031)
                     /locus_tag="VF954_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9530343.1"
                     /translation="MSTSSATFGPSALATPANAVTIARLALAPILVATIVDRGPSWVT
                     LALWFLLAVTDGVDGWVARRQGATRSGAFLDPLADKVLVLGAMFALVGRGRFWLAPVV
                     LLASREVGVSAYRAWAGQRGVSIPARWGAKLKTVVEDLAVGFAVMPATADHRLVATTV
                     LWVSVLLALWTGGQYLADARTMAGADIRAV"
     gene            complement(1028..2338)
                     /locus_tag="VF954_04340"
     CDS             complement(1028..2338)
                     /locus_tag="VF954_04340"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HEX9530344.1"
                     /translation="MPGRYWIETLGCPKNTVDSDKLAGTLETDGYAPADDVGSADIVV
                     VNTCAFIEAARQESIDTILALGDARRPGARLVVTGCLAERYGRELARALPEADQVAGF
                     GVPVTVGGRRHEGSRNVPPLDLLNLPRPPARAPWAYLKVAEGCDRTCGFCAIPSFRGK
                     QRSRALRDIVEEAGALGVAEVVLVAQDLASYGRDQGRAGAIGPLVRAVAGSVQRVRLL
                     YLYPSDLTDGLIDVICETGLPYFDLSLQHAAKPLLRAMRRWGDGDRFLARIADIRRRE
                     PRAAFRSSFIVGYPGETEGDHDELLGFLADAQLDWAGFFAFSPEAGTYAEGLAGRVPD
                     GLVRERLQEASELQEGITASRRSALIGETVEVLVDRPGRSRSHREAPEIDGIISVPTQ
                     LPVGQVGPVAITGAAGPDLEAVAVGAGSGSWAAMASAPDVRSRA"
     gene            complement(2421..>2705)
                     /locus_tag="VF954_04345"
     CDS             complement(2421..>2705)
                     /locus_tag="VF954_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9530345.1"
                     /translation="TSLLVGLPGMQLLDSLAVDGEGHVCVGTLVHAGITDISPDGEVV
                     HHALPPEVFDPLVTNICFGGEHLRTAYLTLSGTGRLVACDWPRPGLELAF"
BASE COUNT          400 a          989 c          992 g          324 t
ORIGIN      
        1 ctggaacggc gtagatgacc ttccccgggc gcggccccga agcgccgacc tggcagatca
       61 gccccggcgc ggtgccctgg gtctgcggga tggggatggc ccccaccggg acgtcggcct
      121 ggcgcaggtt gttggccggc atggaccgcc ctcgcgccat gaagagggcc tcgatggccc
      181 caaccaagga ctggtcgcgc tcgagggcaa cccccagcag cgaggcgatc gcctcccgtg
      241 tgatgtcgtc ctgcgtcggg cccaggccgc cgcagcagat gacggcgtcg ctgcgcccta
      301 gcgccacccg cagcgccgcc acgatgcggc cctggttgtc gcccaccttg gtctggtagt
      361 gcgagtcgat gccggaaagg gccagctgct cgccgatcca ggcggagttg gtgtcgacga
      421 tctggccgag cagcagctcg gtgccgatcg cgaggacctc acaccgcacg aatgtcggca
      481 ccggccatgg tgcgagcgtc cgccaggtac tggcctcccg tccagagcgc cagcagcaca
      541 gagacccaga ggacggtcgt ggccacgagg cggtgatcgg cggtggcggg catgaccgcg
      601 aagccgaccg ccaggtcctc gaccacggtc ttgagcttgg caccccaccg ggcggggatg
      661 gagacgcccc gctgcccggc ccaggcacgg taggcgctga cgcccacctc gcgggaggcg
      721 aggagaacga ccggggccag ccagaaccgg cccctcccga ccagggcgaa catcgccccg
      781 agcaccagca ccttgtcggc cagcggatcg aggaacgccc ccgaccgcgt ggcgccctgg
      841 cggcgggcca cccacccgtc cacgccgtcg gtgaccgcca gcaggaacca cagcgcgagc
      901 gtgacccacg agggcccgcg gtctacgatg gtcgccacca ggatcggggc cagggccagc
      961 cgcgcgatcg tcacggcgtt ggcgggggtg gcgagggcgg acggaccgaa ggttgcgctc
     1021 gacgtgctca agccctcgac cttacgtctg gcgccgacgc catggccgcc cacgacccgc
     1081 tcccggcgcc gaccgccacc gcctcgaggt cgggcccggc ggcacccgtg atggccaccg
     1141 gtccgacctg gcccacgggc agctgcgtgg ggacggagat gatgccgtcg atctccggcg
     1201 cctcccggtg gctgcggctc cggccggggc ggtccaccag gacctcgacc gtctcgccga
     1261 tgagggcgga gcggcgggac gcggtgatgc cctcctgcag ctccgacgcc tcctggaggc
     1321 gttcccggac cagcccgtcg gggacgcgcc cggcgagccc ctcggcgtag gtgcccgcct
     1381 ccgggctgaa ggcgaagaag ccggcccagt cgagctgggc gtcggcgagg aagcccagca
     1441 gctcgtcgtg gtcgccctcc gtctcacccg gatagccgac gatgaaagac gaccggaacg
     1501 ccgctcgggg ttcgcgccgg cggatgtcgg cgatccgggc caggaagcgg tcgccgtcgc
     1561 cccaccggcg catggcccgc agcaacggct tggcggcgtg ctggagggaa aggtcgaagt
     1621 acggcagccc ggtctcgcag atcacatcga tcagaccgtc ggtgaggtcc gatgggtaca
     1681 ggtagaggag gcggacccgc tggaccgacc cggccacggc gcgcacgagc ggcccgatgg
     1741 cccccgcccg cccctggtcc cggccgtagg aggcgagatc ctgggcgacg aggaccacct
     1801 cggccacgcc cagggccccg gcctcttcca ctatgtcccg gagggcccgc gaccgctgct
     1861 tgccccggaa cgacgggatg gcgcagaagc cgcacgtccg gtcgcagccc tcggcgacct
     1921 tgaggtaggc ccagggggcc cgggcgggcg gccgcggcag gttgagcagg tccagcggtg
     1981 ggacgttgcg gctcccctcg tgccggcgtc cgccgactgt cacgggcacg ccgaagccgg
     2041 ccacctggtc ggcctccggc agcgcccggg ccagctcccg cccgtagcgc tcggccaggc
     2101 agccggtcac gaccagccgg gcgcccggcc ggcgggcatc tccgagggcg aggatggtgt
     2161 cgatcgactc ctggcgggcg gcctcgatga aggcgcaggt gttgacgacg acgatgtccg
     2221 ccgacccgac gtcgtcggcc ggggcgtagc cgtccgtctc gagcgtcccc gccagcttgt
     2281 ccgagtcgac ggtgttcttg gggcacccga gcgtctctat ccagtagcgc cccggcacga
     2341 gccagcagtg taccgccggg gagggcgtcc ccccggtggc gcatggcgcg gcgcacggcc
     2401 cgtcatgcgc gccccgggcg tcagaaggcc agctcgagcc cgggcctggg ccagtcgcag
     2461 gccaccagcc gaccggtgcc cgacagcgtg aggtaggcgg tgcggagatg ctcgccgccg
     2521 aagcagatgt tggtcaccag cgggtcgaac acctccggtg gcagcgcgtg gtgcaccacc
     2581 tcgccgtcag gcgagatgtc cgtgatgccg gcatggacca gcgtccctac gcacacgtgc
     2641 ccttcaccgt ccacggcaag ggagtcgagc agctgcatgc cgggaagccc gacgagaagc
     2701 gaagt
//
LOCUS       DASWGG010000240         3766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1513981, whole genome shotgun sequence.
ACCESSION   DASWGG010000240 DASWGG010000000
VERSION     DASWGG010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513981"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VF954_04350"
     CDS             <1..564
                     /locus_tag="VF954_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9530346.1"
                     /translation="ETISGTIPGYLAGTWDLDPVHSEVGFSVRHLMVSKVRGKFTGFT
                     GSFTTADDPLRSSVEVEIDLGSVDTGNEQRDDHLRSADFFAADAHPKLTFSSTGVRPQ
                     GREWIVDGELSLNGVTRPVQLAVEVNGFGPDPYGGTRSGFSATAEISRKDFGIAWNAP
                     LETGGVVIGDKVQVAIEVEGVLRPPAA"
     gene            complement(605..1204)
                     /locus_tag="VF954_04355"
     CDS             complement(605..1204)
                     /locus_tag="VF954_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9530347.1"
                     /translation="MLPSSPAEEVPMGEDAIRPEGPRWRGINHLALVTTDMDATVRFY
                     HGVLGARLVATIGTPSFRHYFFELGIENTVAFFEYHHVDLQPFAKPAGVPDARAVQFD
                     HVSFDLPDEEALLSLRAQLKAAGSEVTDVVDHGFIRSVYFTDPNGIALEASWWTLDAT
                     GRPADYGDERLFADPDPVPAVRELVASGHVTEVPRTRLA"
     gene            1336..2100
                     /locus_tag="VF954_04360"
     CDS             1336..2100
                     /locus_tag="VF954_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9530348.1"
                     /translation="MADVQVEDPAAGGAAGDEASALHRQYAAGRAAVDLHTLVERYRP
                     LARSMAHRFCRSPSDLDDLSQVAYLGLVKALKRFEPDRGFSFTTYAWSTILGEMKRWY
                     RDRNWGVRPPRRVQERYLAVSRTMDELTQLLGRSPTLIEVADRLNVDECDVVEAMEAW
                     RARSVASIDAPSADGAPSPEVGEDDHLELVVNRLEVERLLQCLSPRERRIVELRFFAG
                     CSQAAIAREVGLSQMHVSRLLTGSLARLRRAAEGRP"
     gene            2125..>3766
                     /locus_tag="VF954_04365"
     CDS             2125..>3766
                     /locus_tag="VF954_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9530349.1"
                     /translation="MSPGAGGGPPTGLAFAVLPPVLESVARARLLVADTASRWGLAGL
                     REDASLVASELATNAVLHGRTAFRVSVRSRPSGMRIEVWDASDDPVVAPIDPPGPGGA
                     GTLLDEPDDRLDVEPLLTSGITGRGLALVQAVAAAWGVDEGREGGKTVWAELGARSPF
                     GPMPPAARPVAAPPRASGGQRTARLVAVPVDLVLGSEQHLSDVLRELQVMCMGGAGGP
                     DDRPVAALRELLEHRAPWRTAMEEASRQAVARGDQLVDVTLRLTAAAVAWLEAVDRAG
                     AALLDTSRGAAVVSLAPAPQVAAFRRWWAQEVSAQLAGRAPRACPLPGGGWGPMQPGD
                     GGDTGGGGWAADGGPVAGPDADAERAALNARRQVELADLARRLDHVSGAGEVAAAVLE
                     RLLVTVGATAASLCLLGPDHETVEVTHAVGQPDERATRPRAFQLSAAHPACEAIRTGR
                     TVAVRTGAERDGRFPDLSDDAMAAGNALACTPLLGTAGAGGAGGAAGAAGSVRSGVGA
                     VGALVISFPRARDFTQGDVEFLEAISSVMAQALGSARAA"
BASE COUNT          470 a         1319 c         1449 g          528 t
ORIGIN      
        1 gaaaccattt ctgggaccat cccgggttac ctggcaggga cctgggacct cgatccggtg
       61 cactcggagg tgggcttcag cgtccgccac ctgatggtgt cgaaggtgcg gggcaagttc
      121 accgggttca cggggagctt caccacggcg gacgacccgc tgcggtcgtc ggtcgaggtg
      181 gagatcgacc tgggctcggt cgacaccggg aacgagcagc gcgacgacca cctgcgctcc
      241 gccgacttct tcgccgccga cgcccatccc aagctgacgt tctcgtcgac cggcgtgcgc
      301 ccgcagggca gggagtggat cgtggacggc gagctcagcc tcaatggggt gaccaggccg
      361 gttcagctgg ccgtcgaggt gaacgggttc ggcccggatc cctacggcgg gacccgcagc
      421 gggttctccg ccaccgccga gatcagccgg aaggacttcg gcatcgcctg gaacgctccc
      481 ctcgagaccg gcggggtggt catcggtgac aaggtccagg tggccatcga ggtcgagggc
      541 gtgctgcggc cgccggccgc ctgaggcgtc tccgtaccaa gggcgccacc ggatctgccg
      601 cggctcaggc gagcctggtg cgcggcacct cggtgacatg gcccgaggcc accagctctc
      661 ggacggccgg taccgggtcg ggatcggcga agaggcgctc gtccccgtag tccgccggcc
      721 gcccggtggc gtcgagggtc caccacgagg cctccagggc gatgccgttc gggtcggtga
      781 agtacaccga gcggatgaac ccgtggtcca ccacgtcggt gacctccgag ccggccgcct
      841 tcagttgcgc ccgcagcgaa aggagggcct cctcgtcggg caggtcgaac gagacgtggt
      901 cgaactgcac ggcccgcgca tccggcacgc ctgccggctt ggcgaagggc tggaggtcga
      961 cgtggtggta ctcgaagaac gcgaccgtgt tctcgatccc gagctcgaag aagtagtgcc
     1021 ggaacgacgg cgtgccgatg gtggccacca gccgggcccc gagcaccccg tgatagaagc
     1081 gtacggtggc atccatgtcc gttgtcacga gggccaggtg gttgatccct cgccagcggg
     1141 gcccctcggg ccggatggcg tcttcgccca tgggcacctc ctcggcagga ctcgacggta
     1201 acaccttgaa aggggagaaa gggggaagcg gtcgccggtc ggatcggagg ccctggtttc
     1261 accgcggttg ccggggggta cgccttcccc agccgccggc cgctggtatg tcgcgcccgg
     1321 cagaaggaga cccgggtggc tgacgtgcag gtcgaagacc ccgccgccgg cggcgcggcc
     1381 ggagacgagg cgtccgcgtt gcaccgccag tacgcggccg ggcgcgccgc ggtcgacctg
     1441 cacacgctcg tcgagcggta ccggccgctg gcccggtcga tggcccacag gttctgccgg
     1501 tcgccgtcgg acctggacga cctgtcgcag gtggcctacc tggggctggt caaggcgctg
     1561 aagcgtttcg agccggaccg ggggttctcg ttcactacct atgcctggtc caccatcctc
     1621 ggcgagatga agcggtggta ccgggaccgc aactgggggg tccggccgcc ccgcagggtc
     1681 caggagcgct atctggccgt ctcccgcacc atggacgagc tcacgcagct gctgggccgg
     1741 tcgcccacgc tgatcgaggt cgccgaccgt ctcaacgtcg acgagtgcga cgtcgtggag
     1801 gccatggagg cgtggcgggc acggtcggtc gcgtccatcg acgcgccgag cgccgatggc
     1861 gcacccagtc ccgaggtcgg cgaagacgac cacctggagc tcgtcgtcaa ccggttggag
     1921 gtggaacggc tgctccagtg cctctcgccg cgggagcgtc ggatcgtgga gctgcggttc
     1981 ttcgccgggt gctcgcaggc agccatcgcc cgcgaggtcg ggctttcgca gatgcacgtc
     2041 tcccgcctcc tcaccggcag cctggcccgg ctccggcggg ccgccgaggg ccggccgtga
     2101 gagctcggcc gtgagagcac cgctgtgagc cccggcgcag ggggcggtcc gcccaccggc
     2161 ctggccttcg ccgtgctgcc gcccgtgctg gagagcgtgg cccgggcccg cctgctcgtg
     2221 gcggacacgg cctcgcggtg ggggcttgcc ggcctgcggg aggacgcgtc tctggtggcc
     2281 tccgagctgg cgaccaacgc cgtgctccac ggccgcaccg cctttcgggt ctcggttcgg
     2341 tcacgcccgt ccgggatgag gatcgaggtc tgggacgcca gcgacgaccc cgtcgtggcg
     2401 ccgatcgacc cgccgggacc aggaggggcc gggacgctgc tcgacgagcc ggacgaccgc
     2461 ctcgacgtcg agccgctgct cacgtcgggg atcaccggtc ggggcttggc cctggtccag
     2521 gccgtcgccg ccgcgtgggg tgtcgacgag ggtcgcgagg ggggcaagac ggtgtgggcc
     2581 gagctgggtg cgcgctcccc gttcggcccg atgccgccgg cggcgcggcc ggtggcggcg
     2641 ccgcccaggg cgtccggtgg tcagcggacg gcgcggctcg tcgccgtgcc cgtggacctg
     2701 gtcctcgggt ccgaacagca cctgagcgac gtcctgcggg agctccaggt catgtgcatg
     2761 ggcggcgccg gcgggcccga cgaccggccc gtggcggccc tgcgggagct cctcgagcac
     2821 cgcgccccgt ggcggaccgc catggaggag gcgtcgcgcc aggccgtggc gcgcggcgat
     2881 cagctcgtcg acgtcacgct ccgcctgacc gcggccgccg tcgcctggct ggaggcggtc
     2941 gaccgggccg gtgcggcctt gctcgacacg tcgaggggcg ccgccgtcgt ctctctggcg
     3001 cccgcgccgc aggtggcggc cttccggcgc tggtgggccc aggaggtgtc ggcgcagctg
     3061 gccgggcgtg cgccccgagc ctgtcccctc ccaggcgggg gatgggggcc gatgcagccg
     3121 ggggacggtg gcgacaccgg cggcggtggg tgggccgccg acggcgggcc ggtcgccggt
     3181 cccgatgccg acgccgagag ggccgcgctc aacgctcggc gtcaggtgga gctggctgac
     3241 ctggcccgcc gcctcgacca cgtgtcgggt gcgggggagg tggcggcggc ggtgctcgag
     3301 cgcctgctcg tcaccgtggg agcaaccgcg gcatctctgt gcctgctcgg ccccgaccac
     3361 gagaccgtcg aggtcaccca cgccgtcggc cagcccgatg agcgcgccac ccggccacgc
     3421 gctttccagc tgtcggctgc ccacccggcg tgcgaggcca tcaggaccgg acgcaccgtg
     3481 gccgttcgca ccggcgccga acgcgacggg cggttccccg acctgtccga cgacgccatg
     3541 gcggccggaa atgcgctggc gtgcacgccg ttgctgggca cggccggcgc gggcggcgcg
     3601 ggcggcgcgg ccggcgcggc cggctcggtc cgctcgggcg tgggggcggt gggggcactc
     3661 gtcatcagct ttccccgggc ccgggacttc acccagggag acgtcgagtt ccttgaggcg
     3721 atctcctcgg tcatggctca ggcgctcggc tcggcccggg ctgcga
//
LOCUS       DASWGG010000241         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_871108, whole genome shotgun sequence.
ACCESSION   DASWGG010000241 DASWGG010000000
VERSION     DASWGG010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871108"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VF954_04370"
     CDS             complement(<1..676)
                     /locus_tag="VF954_04370"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530350.1"
                     /translation="MVALTKGVLRSLATFKSSGPPVVSLYLDVDGHRYVRARDYEVEL
                     DALLRRAAAGPAAADLSRIEGFVKVGVDRSRTRGLAIFSCAAEGLWEVIGLPVPVHNR
                     LTVDHAPQVRQLEAVLDSNDRFGVLLVDRQRARLFVFELGELVERSELFDQLPRHEDD
                     KGEWDRDHVHDHQATAARAHLRKAAQVAFDADRAQAFDHLVLGGPSEVVADLERELHS
                     YLHHRIA"
     gene            complement(721..1011)
                     /locus_tag="VF954_04375"
     CDS             complement(721..1011)
                     /locus_tag="VF954_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530351.1"
                     /translation="MSQPREESARQALEHLQRAVLETIAAARSALDVAEELVRDPAHL
                     AGLAAAMATIADAVGGIMNDAMTARRHEPGGRPGDNGANHRADRNVEHISVG"
     gene            complement(1008..2192)
                     /locus_tag="VF954_04380"
     CDS             complement(1008..2192)
                     /locus_tag="VF954_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX9530352.1"
                     /translation="MRLLLFTGKGGVGKTSVAAATALLAADAGLRTLVLSTDPAHSLA
                     DALDLPLGSDPTAVVPRLWGQQLDAAERLEDAWGGVQAWLAQLLDWAGVDAIEAEELA
                     VLPGLDEIFSLADIKDHAASGAWDVIIVDCGPTAETIRLLSLPDVLRWYMERVFPVER
                     TVTKLIRPLLQRLSDLPVAGDDVFQAAVRFYGRLDGVRALLTDRDVASVRLVVNPERV
                     VLAEARRTATYLALFGYRVDAVIANRVLPDVVTDPWFRAWKDVHAGHLAAIHDEFDPI
                     PVLRADLVGAELVGVDHLRSFGRHLYSAVEPTAVLHTGDLLTLAADGDERVLDVPLPF
                     VDRDELEVGRSDEDLLVRVGPHRRAICLPDSLRGRSVVSANLDGDRLRVRFGHHGQGE
                     VP"
     gene            complement(2189..>2540)
                     /locus_tag="VF954_04385"
     CDS             complement(2189..>2540)
                     /locus_tag="VF954_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9530353.1"
                     /translation="AADIKRVTVLARDGDGRATQVAFRTAAFGRSTNYTLAYDYELAP
                     REVAWVLINGDLTNKLDGAYIFDPAGDGDTEVTYRLVAELKIPLPGFIKRRAESRIIK
                     TALKQLRPRVESGS"
BASE COUNT          368 a          880 c          951 g          341 t
ORIGIN      
        1 ccgcgatgcg gtggtggagg taggagtgca gctcccgctc gaggtcggcc accacctccg
       61 acgggccccc gaggacgagg tggtcgaaag cctgggcccg gtcggcgtcg aaggccacct
      121 gggcggcttt gcgcaggtgg gcccgggccg cggtggcctg gtggtcatgc acgtggtcgc
      181 ggtcccattc gcccttgtcg tcctcgtggc ggggaagctg gtcgaacagc tcgctgcgct
      241 cgaccagctc gcccaactcg aagacgaaca gccgggcccg ctggcgatcc accagcagca
      301 cgccgaagcg gtcgttcgag tcgagcaccg cctccagctg gcggacctgg ggggcgtggt
      361 cgacggtcag gcggttgtgg accggcacgg ggagcccgat gacctcccac aggccctcgg
      421 ccgcgcagga gaagatggcc aggccccggg tgcgggagcg gtccacgcct accttgacga
      481 acccctcgat ccggctcagg tcggcggcgg ccggcccggc ggcggcccgc cgcaggaggg
      541 cgtccagctc gacctcgtag tcacgggccc ggacgtagcg gtgcccgtcg acgtccaggt
      601 acaggctcac gacgggggga ccggagctct tgaacgtggc cagagagcgg aggacgccct
      661 tggtgagtgc aaccacgcca cctcctaggt ccggggagtc gatggtacgc cgccgccggc
      721 tcagccgacg gaaatgtgct ccacgttgcg atcggctctg tggttggctc cgttatcgcc
      781 cggacggccc ccgggctcgt ggcgccgcgc cgtcatggcg tcgttcatga tcccgccgac
      841 cgcgtcggca atcgtggcca tggccgcggc cagcccggcc agatgggcgg ggtcccggac
      901 gagctcctcg gccacgtcca gcgccgaacg ggccgcggcg atggtctcga ggaccgcccg
      961 ctggaggtgc tcgagtgcct ggcgcgccga ctcctctctc ggttggctca tggcacctct
     1021 ccctggccgt ggtggccgaa ccggacgcgc agccggtcgc cgtcgaggtt ggccgacacc
     1081 accgagcggc cccgcagcga gtcgggcagg cagatggccc gccgatgggg ccccacccgc
     1141 acgagcaggt cctcgtccga ccggccgacc tcgagctcgt cacggtcgac gaacggcagc
     1201 ggcacgtcca ggacgcgctc gtcgccgtct gccgccaggg tgagcaggtc gccggtgtgc
     1261 agcacggcgg tcggctcgac agcggagtac aggtgacggc cgaaggagcg aagatgatcg
     1321 acgccgacga gctcggcgcc gacgaggtcc gcccgcagga cagggatggg gtcgaactcg
     1381 tcgtggatgg cggccaggtg tccggcatgg acgtccttcc acgcccggaa ccaggggtcg
     1441 gtgaccacgt cgggcaggac ccggttggcg atgaccgcgt cgacgcggta gccgaagagg
     1501 gcgaggtagg tggcggtgcg gcgggcctcg gccagcacga cgcgctccgg gttgacgacc
     1561 aggcgcacgc tcgccacatc gcggtcggtc aggagggccc ggaccccgtc cagcctcccg
     1621 tagaaccgaa cggccgcctg gaacacgtcg tcgccggcga cgggcaggtc gctgagacgc
     1681 tggagcaagg gccggatcag cttggtgacg gtgcgctcga cggggaacac ccgctccatg
     1741 taccagcgca ggacatcggg cagcgacagc agccggatgg tctccgcggt agggccgcag
     1801 tcgacgatga tgacgtccca cgcgcccgag gcggcgtggt ccttgatgtc ggcgaggctg
     1861 aagatctcgt ccagcccggg cagcacggcg agctcctcgg cctcgatggc gtccacgccc
     1921 gcccagtcga ggagctgggc cagccacgcc tgcacgccgc cccaagcatc ctccaggcgc
     1981 tcggcggcgt cgagctgctg gccccagagc cggggcacca cggccgtcgg atccgagccg
     2041 aggggcaggt cgagcgcatc ggccagcgag tgggccggat cggtcgagag cacgagcgtg
     2101 cgcagcccgg cgtcggcggc cagcagggcg gtggccgcgg cgacgctcgt cttgccgacg
     2161 ccgcccttgc cggtgaacag cagcagcctc acgagccaga ctcgacccgg ggtcgcagct
     2221 gcttgagggc ggtcttgatg atgcggctct cggcccggcg cttgatgaag cccgggagcg
     2281 ggatcttcag ctcggccacc aaccggtacg tgacctcggt gtcgccgtcg cctgccgggt
     2341 cgaagatata ggcaccgtcc agcttgttgg tgaggtcccc gttgatgagc acccaggcca
     2401 cctcgcgggg cgccagctcg tagtcgtagg ccagggtgta gttggtgctg cgcccgaacg
     2461 cggccgtgcg gaacgcgacc tgggtggccc gaccgtcgcc gtcccgggcc agcacggtga
     2521 cccgcttgat gtccgcggcc
//
LOCUS       DASWGG010000242         2929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1199653, whole genome shotgun sequence.
ACCESSION   DASWGG010000242 DASWGG010000000
VERSION     DASWGG010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199653"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VF954_04390"
     CDS             complement(<1..1250)
                     /locus_tag="VF954_04390"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9530354.1"
                     /translation="MTDSGFTVDVDQNQYLPEGGRDVNAVVTVTSGADVSVAAPGGGA
                     GSAEIIIIDCSGSMDYPPTKMSQARAATAAAIDVVRDGVGFAVVAGTSTAWPVFPSDG
                     SMAVANPASRAAAKQAVTGLRANGGTAIGQWLGLARRIFAGYPAQLRHAILLTDGKNQ
                     HETPGQLAAAIAQCEGTFSCDCRGVGTDWEVKELRTVSTTLLGTVDIVPDPAGLAADF
                     ASMMENAMGKQVADVSLRVWTPQTATLKFVKQVAPAVEDLTGRRTQTGPQAGDFPTGA
                     WGPGESRDYHVGVEVNPAGVGQEMLAARISLVAGAEVLGQGLIRAIWTDDEALSTKIN
                     PRVAHYTGQAELAEAIQEGLEARKQGDEETATAKLGRAVALAHQSGNEDTAKLLAKVV
                     DVVDVESGTVRLKKKVEDADEMAL"
     gene            complement(1247..2281)
                     /locus_tag="VF954_04395"
     CDS             complement(1247..2281)
                     /locus_tag="VF954_04395"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX9530355.1"
                     /translation="MHDGNTGVTCPACGSQAGAADSFCESCGTELAPPVSSAGPPGYS
                     AQCPVCSLDESAAPGAISAEGYCESCGRKVPSGRDHVELNLEILAGVTDRGLRHPRNE
                     DAMALATTETPDGPVAIAVVCDGVSSSPNPEDASLTGAQAAVRMLLTSIRRGDDPDEA
                     SMAGVRQAHEAMAILGSPSRAPSATYVSVIVGADAVTVCWLGDSRAYWLATESESQRL
                     TRDDSLAEELVADGEMTEAQAMASPQAHVITRWLGADVVTPQPHIARFEPPGHGVILV
                     CSDGLWNYRPEAEGLAELTLPEAFNDPTSAADALLKFALEAGGMDNITVVLVPFPLVR
                     PAPLLKEAPS"
     gene            complement(2308..2796)
                     /locus_tag="VF954_04400"
     CDS             complement(2308..2796)
                     /locus_tag="VF954_04400"
                     /inference="COORDINATES: protein motif:HMM:NF028228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9530356.1"
                     /translation="MAGLPLPQVDRSHISAAFGLARICLAAGDRPGAIAALAGVPGTS
                     SHHAAAQIAAVRILVAGAGPGKVSGDDLRQADGRLGQLVVDDVRRQQLTVEILRAALD
                     WCSTGQPSGNGSILGCEPDARALRFGLERSYRALARLTPDEGRRIELVDMANTIRPRT
                     LT"
BASE COUNT          361 a         1064 c         1079 g          425 t
ORIGIN      
        1 tcgagggcca tctcgtcggc gtcctcgacc ttcttcttca gccggaccgt gccggactcc
       61 acatccacca catctaccac cttggccagc agcttggcgg tgtcctcgtt gcccgactgg
      121 tgggccagcg ctaccgcccg gccgagcttg gcggtggcgg tctcctcgtc gccctgcttg
      181 cgggcctcca gcccctcctg gatggcctcg gcgagttcgg cctgcccggt gtagtgcgcc
      241 acccgcgggt tgatcttggt ggagagcgct tcgtcgtcgg tccagatcgc gcggatcagg
      301 ccctggccga ggacttctgc gccggcgacc aggctgatcc gcgcggccag catctcctgg
      361 ccgacgccgg ccgggttgac ctccacgccg acgtggtagt cccggctctc ccccggcccc
      421 caggccccgg tcgggaagtc accggcctgc ggacccgtct gggtacgcct gccggtcagg
      481 tcctcgaccg cgggcgcgac ctgcttgacg aacttgagcg tggcggtctg cggcgtccac
      541 acccgcagtg acacgtcggc gacctgcttg cccatcgcgt tctccatcat cgacgcgaag
      601 tcggccgcca gcccggccgg gtcgggcacg atgtccaccg tgccgagcag cgtggtggag
      661 accgtgcgca gttccttgac ctcccagtcg gtgcccacgc cgcggcagtc gcagctgaag
      721 gtgccctcgc actgggcgat cgccgcggcg agctgaccgg gggtctcgtg ctggttcttg
      781 ccgtcggtga gcaggatggc gtgccgcagc tgcgccgggt agccggcgaa gatccgccgg
      841 gccagcccca gccactggcc gatggcggta ccgccgttgg cgcgcagccc ggtcaccgcc
      901 tgcttggccg cggcccgcga cgccgggttc gcgaccgcca tgctcccgtc ggaaggaaag
      961 accggccagg cggtgctggt ccccgcgacg accgcgaatc ccacgccgtc gcggaccacg
     1021 tcgatcgcgg cggcggtggc ggcgcgcgcc tggctcatct tggtcggcgg gtaatccatc
     1081 gatccggagc agtcgatgat gatgatctcg gcactgcccg cgcctccgcc cggagccgcc
     1141 acggagacgt ccgcccctga cgtcacggtc accacggcgt tcacgtcgcg cccgccctcg
     1201 ggcaggtact ggttctggtc gacgtcgacg gtgaagccgg agtcggtcat gacggagcct
     1261 ccttgagtaa cggcgcgggc cgcacgagcg ggaacgggac cagcacgacg gtgatgttgt
     1321 ccatgccgcc ggcctcgagg gcgaacttca gcagcgcgtc ggccgcgctg gtcggatcgt
     1381 tgaacgcttc cgggagggtg agctcggcca ggccctccgc ctcgggccgg tagttccaca
     1441 gcccgtcgga gcagacgagg atgacgccgt gcccgggcgg ctcgaaccgg gcgatgtgcg
     1501 gctgcggggt gaccacgtcg gcaccgagcc agcgggtgat cacgtgcgcc tggggggagg
     1561 ccatggcctg cgcctcggtc atctcgccgt cggcgaccag ctcctcggcg agcgagtcgt
     1621 ccctggtcag gcgctgggac tcggactcgg tggccagcca gtaggcgcgg ctgtcgccga
     1681 gccagcagac cgtgaccgcg tccgcgccga cgatcaccga tacgtaggtg gccgacggcg
     1741 cgcgggacgg cgagcccaga atggccatgg cctcgtgggc ctggcgcacc ccggccatgg
     1801 acgcctcgtc cgggtcgtcg ccgcggcgga tcgaggtgag cagcatgcgc accgccgcct
     1861 gcgccccggt cagcgacgcg tcttccgggt tcggcgagga cgacacgccg tcgcagacca
     1921 cggcgatcgc caccgggccg tccggggtct ccgtggtcgc gagggccatc gcgtcctcgt
     1981 tgcgcggatg ccgcaggccg cggtcggtga ccccggccag gatctcgagg ttgagctcga
     2041 cgtggtcgcg gccggacggc accttgcggc cgcacgactc gcagtagccc tcggcgctga
     2101 tcgcgccggg agcggccgac tcgtcgaggg agcacaccgg gcactgcgcg ctgtacccgg
     2161 gcggcccggc gctgctgacg ggcggggcga gctcggtccc gcatgactcg cagaagctgt
     2221 ccgcggcgcc ggcctggctg ccgcaggccg ggcaggtgac tcccgtattg ccatcgtgca
     2281 tcaggccatc atccagggca ccggccgtca tgtcagcgtc cggggccgga ttgtgttggc
     2341 catgtcgaca agttcgatcc ggcggccctc gtccggggtc agccgggcca gcgcccggta
     2401 gctccgctcg agcccgaaac gcagtgcccg ggcgtccggt tcgcagccca ggatggagcc
     2461 gttccccgac ggctggccgg tgctgcacca gtcgagcgcg gcccgcagga tctccaccgt
     2521 gagctgctgc ctgcgcacgt cgtccacgac cagctggccg agccggccgt cggcctggcg
     2581 caggtcgtcg ccggagacct tacccgggcc ggcgccggcc accaggatgc ggaccgcggc
     2641 gatctgcgcg gccgcgtggt ggctcgaggt gccgggcacg cccgccaggg ccgcgatcgc
     2701 gccgggccgg tcgccggccg ccaggcagat cctggccagg ccgaacgcgg cgctgatgtg
     2761 ggaccggtcc acctgcggga gcggcagccc cgccacgatt tccgccccgg tggggcgcag
     2821 gaccggactg gggggacttg tcccccccaa ggggggcccc gggggtatgg gggggcgggc
     2881 agcccctccc aaggggggtc cggggggatg ggtccctccc aaggggggt
//
LOCUS       DASWGG010000243         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3128842, whole genome shotgun sequence.
ACCESSION   DASWGG010000243 DASWGG010000000
VERSION     DASWGG010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3128842"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VF954_04405"
     CDS             complement(<1..749)
                     /locus_tag="VF954_04405"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530357.1"
                     /translation="MGHANLVVHGHFYQPPRENPWTEEVAREASAGPFHDWNARITAE
                     CYRPNGWARVIDDRGRVVAIVNNYEHLSFDMGPTLMSWLATEAPETYARVLEADRRTG
                     RAIAQAYGHMILPLATPRDLRTQVRWGLADFAHRFGRPAAGMWLPETAVNDAVLAALA
                     EEGVAFTILAPNQAAAVRRIDGVQEQLLVDGADAGWQEISDRSLDTSSTYRWTHPDRR
                     SLGVDIVFYDGELAHAMAFGLAGMSSEQLVDR"
     gene            complement(807..3020)
                     /gene="glgB"
                     /locus_tag="VF954_04410"
     CDS             complement(807..3020)
                     /gene="glgB"
                     /locus_tag="VF954_04410"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX9530358.1"
                     /translation="MAASRATPAPDAATEILPAALQQLVDGEHGDPHRILGFHEGVVR
                     AYRPGAASLRVLLAGGGGPVAMRAVHDAGLFEASCPPAEAGYRLEATYPDGEVVAFDD
                     PYRCWPTLGELDVYLLGEGRHRRIWQVLGARHRVHEGLAGTSFAVWAPNARSVRVVGD
                     FNLWDGRLHPMRALGSSGVWELFLPGVAPGARYKYEIVTPGHELRLKADPVARAAEVP
                     PGTASVVAAPDAYVWNDREWLERRSTGDPQAAPMSVYEIHLGSWRRVPEDRDRPLTYA
                     ELADQLADYVVGMGFTHVEFMPVAEHPFGGSWGYQVSSYFAPTARYGEPDELRALVDT
                     LHARGVGVLVDWVPAHFPRDDWALARFDGTALYEHDDPRKGTQPDWGTLVFNLGRNEV
                     RNFLISNALYWIEEFHVDGLRVDAVASMLYLDYSRKDGEWVPNQYGGRENLDAVGFLK
                     EMNETVYGEHPGVVTVAEESTAWPAVSRPTYVGGLGFGLKWNMGWMHDTLEYFSKEPI
                     HRRYHHNELTFGLLYAFSENFVLPLSHDEVVHGKGSLLAKMPGDRWQQLANLRALLAW
                     MWAHPGKQMLFMGGEIAQEREWSHDRSLDWHLLSVPGHAGVQHLVRALNAAYREAPAL
                     WQRDSAPDGFRWIDASDVEHGVLSFLRLGEAPSQPLVCIANLTPVPRHGYRIGLPTGG
                     AWRELLNTDASDFGGSGVGNGGAVWAGEDGWHGLPASAELSLPPLGVLWLAPADQ"
     gene            complement(3044..>3634)
                     /locus_tag="VF954_04415"
     CDS             complement(3044..>3634)
                     /locus_tag="VF954_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX9530359.1"
                     /translation="PGRADAWAGTIERELGPIASEGLDPRAARDVVDRLRHAKDPGLE
                     IRVHGDYHLGQVMRTDTGWFVLDFEGEPARPVEERAAPWSPLKDVAGMLRSFQYAAAA
                     ALNERGEPERTEALYALGGAWEMHNRGAFLDGYRSTRGISELVPGDDESFDAVLAAFE
                     LEKAIYELAYELAYRPDWALIPRAALERILSERGTS"
BASE COUNT          508 a         1277 c         1341 g          508 t
ORIGIN      
        1 cggtccacga gctgctcgct cgacatgccg gccagcccga acgccatggc gtgggcgagc
       61 tctccgtcgt agaagacgat gtcgacgccg agcgagcgcc ggtcggggtg ggtccaacgg
      121 taggtgctcg acgtgtcgag cgagcggtcg gagatctcct gccacccggc atcggcgccg
      181 tcgacgagca gctgctcctg gacaccgtcg atccggcgca cggccgcggc ctggttgggg
      241 gcgaggatgg tgaaggccac gccctcctcg gcgagggccg cgagcactgc gtcgttgacg
      301 gccgtctccg gcagccacat gcccgccgcc ggccggccga agcggtgggc gaaatcggcc
      361 aggccccatc ggacctgcgt gcgaaggtcc cggggggtgg cgagcgggag gatcatgtgg
      421 ccgtaggcct gggcgatggc ccggcccgtc ctgcggtcgg cctccaggac gcgggcgtag
      481 gtctcgggcg cttcggtggc cagccaggac atgagggtgg ggcccatgtc gaaggagagg
      541 tgctcgtagt tgttcacgat ggccaccacc cggccccgat cgtcgatcac ccgggcccag
      601 ccgttgggcc ggtagcactc ggcggtgatg cgcgcgttcc agtcgtggaa gggccccgca
      661 ctggcctcgc gggccacctc ctcggtccag gggttctccc tgggcggctg gtagaagtgg
      721 ccgtggacga cgaggttggc gtgacccacg agcggagggt accggcccgc agccaggtcg
      781 cccggcgggc ttactggtcg gcgggcttac tggtcggcgg gcgccagcca gagcacaccg
      841 agggggggca gactcagctc ggccgacgcc ggcaggccgt gccagccgtc ctcgccggcc
      901 cataccgcgc ccccgttgcc cacaccgctg cccccgaagt cggaggcgtc ggtgttgagc
      961 agctcgcgcc atgcaccgcc ggtcggcagg ccgatgcggt agccgtggcg ggggacggga
     1021 gtcaggttgg ctatgcacac cagcggctgc gacggtgcct ccccgagacg gaggaacgac
     1081 agcacgccgt gctccacgtc gctggcgtcg atccaccgga acccgtcggg cgcggagtcg
     1141 cgttgccata gcgcgggtgc ctcccggtac gcggcgttga gggcccggac caggtgctgg
     1201 acgccggcgt ggccgggcac ggacagcagg tgccagtcga ggctccggtc gtggctccat
     1261 tcacgctcct gggcgatctc gccgcccatg aacagcatct gcttgccggg gtgcgcccac
     1321 atccaggcca gcagggcgcg caggttggcg agctgctgcc accggtcgcc gggcatcttg
     1381 gcgaggagcg agcccttgcc gtgcacgacc tcgtcgtggg acagcgggag gacgaagttc
     1441 tcgctgaacg cgtacagcag cccgaaggtc agctcgttgt ggtggtagcg ccggtggatg
     1501 ggctccttgg agaagtactc gagcgtgtcg tgcatccagc ccatgttcca cttgagcccg
     1561 aacccgagcc cgccgacgta ggtggggcgc gacacggccg gccacgccgt ggactcctcc
     1621 gccactgtga ccacgccggg gtgctcgccg tagacggtct cgttcatctc cttgaggaag
     1681 cccacggcgt cgaggttctc gcggcccccg tactggttgg ggacccattc cccatccttg
     1741 cgggagtagt cgaggtagag catggaggcg acggcgtcga cccgcagccc gtcgacgtga
     1801 aactcctcga tccagtacag ggcgttgctg atgaggaagt tgcgcacctc gttgcggccc
     1861 aggttgaaca ccagcgtgcc ccagtcgggc tgggtgcctt tccgcgggtc gtcgtgctcg
     1921 tacagggcgg tgccgtcgaa gcgggccagg gcccagtcgt cgcgggggaa gtgcgccggc
     1981 acccagtcga cgagcacgcc gaccccccgg gcgtggaggg tgtcgacgag ggcccgcagc
     2041 tcgtctggct cgccgtagcg ggcggtcggg gcgaagtagg aagacacctg gtagccccac
     2101 gacccgccga agggatgctc ggccaccggc atgaattcca cgtgggtgaa gcccatgccc
     2161 accacgtagt cggcgagctg gtcggcgagc tcggcgtagg tgagcggccg gtcccggtcc
     2221 tcgggcacgc gccgccacga gccgaggtgg atctcgtaca ccgacatcgg cgccgcctgc
     2281 gggtcgccgg tgctgcgccg ctccagccac tcccggtcgt tccacacata ggcgtcgggc
     2341 gcggccacca cgctcgccgt cccgggcggg acctcggcgg cgcgtgccac ggggtccgcc
     2401 ttcaggcgca gctcatggcc cggggtcacg atctcgtact tgtaccgggc gcccggggcc
     2461 acgccgggga ggaagagctc ccagacgccg gaagacccca gggcgcgcat cgggtggagg
     2521 cgcccgtccc acaggttgaa gtcgccgacg acgcgcaccg accgggcgtt cggggcccag
     2581 accgcgaacg acgtgcccgc cagcccctcg tggacgcggt gccgggcacc gagcacctgc
     2641 cagatccggc ggtggcggcc ttccccgagg aggtacacgt cgagctcccc gagggtcggc
     2701 cagcaacggt aggggtcgtc gaacgccacg acctcgccgt ccgggtaggt ggcctccagg
     2761 cggtagccgg cctcggccgg cgggcagctg gcctcgaaga ggccggcgtc gtggaccgcc
     2821 cgcatggcga ccgggccccc gccccctgca agcaggacgc gcagggaggc cgcccccggc
     2881 cgataggcgc gcaccacacc ctcgtggaac cccaggatgc gatgaggatc gccgtgctcc
     2941 ccgtcgacca gctgctggag ggcggcgggc aggatctcag tggcggcgtc gggtgctggc
     3001 gtagctcggc tggcggccat ggggatgtcc ctacccactt cggtcaactc gtgccccgct
     3061 cggacaggat gcgctccagg gcggcgcggg ggatcaaggc ccagtcaggc cggtaggcca
     3121 gctcgtaggc cagctcgtag atcgccttct ccagctcgaa ggcggccagc acggcgtcga
     3181 aggactcgtc gtctccgggc accagctcgg agatgccccg ggtcgagcgg tagccgtcca
     3241 ggaaggcgcc ccggttgtgc atctcccacg ccccgcccag cgcgtagagc gcctcggtcc
     3301 gctcgggctc gccccgctcg ttgagcgccg ccgccgccgc gtactggaac gagcgcagca
     3361 tgcccgccac gtccttgagc ggcgaccacg gcgcggccct ttcctccacc ggccgggccg
     3421 gctcgccctc gaaatcgagc acgaaccatc ccgtgtcggt ccgcatgacc tggcccaggt
     3481 ggtagtcgcc gtggacgcgg atctcgagac ccgggtcctt ggcgtggcgg agacggtcga
     3541 ccacgtcccg tgccgcgcgg gggtcgagac cctccgacgc gatcgggccg agctctcgct
     3601 cgatcgtgcc cgcccatgca tcggcgcggc ccgg
//
LOCUS       DASWGG010000244         6312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1516140, whole genome shotgun sequence.
ACCESSION   DASWGG010000244 DASWGG010000000
VERSION     DASWGG010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516140"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1221)
                     /gene="alr"
                     /locus_tag="VF954_04420"
     CDS             complement(82..1221)
                     /gene="alr"
                     /locus_tag="VF954_04420"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX9530360.1"
                     /translation="MGEPARHFRPAWAEVDLGAIRANASALAAAAAPAQLCAVVKANG
                     YGHGAVPAARAALAGGATWLAVALVEEGRELRDAGIGAPILLLSEPVPDAMDEVVACD
                     LTPTLYSRPAAAALERAARASDRTLDVHFKIDTGMHRVGASPAHAVEAIRAVISSGVL
                     RLAGVWTHLAVADEPEDGFTEHQLQVLEGVRADLEALGIRPPLVHAANSAGALLHPRS
                     RGDLVRCGIGLYGYAPAPGLDGGLRLRPALALKARVTLARGVAAGDRVSYGLRYRLDA
                     DSVVATVPLGYADGVPRRLSATGAHVLIRGRRRPVAGTITMDQLMVDCGPGGGVAAGD
                     EVVLLGDQQGLRITAEDWASRLDTISYEIVCGIGPRVPRVYVGGP"
     gene            complement(1214..2578)
                     /locus_tag="VF954_04425"
     CDS             complement(1214..2578)
                     /locus_tag="VF954_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX9530361.1"
                     /translation="MQPVLTVAEMAEVDALARRSVAVEALIERAGRAVAREARRMLGG
                     TYGRRVVVVAGKGHNGDDGRVAAHRLTAAGARVLLVDAREAPRSLPPCDLVVDAAYGT
                     GFAGEYRAPVASAPVLAVDIPSGVMGDTGEASPEAVRADVTVTFVALKPGLLLGEGRA
                     RAGQVRVADIGLDASDATAHRLEDADLAALPTRPPEAHKWQTALAVVAGSPGMMGAAR
                     LCSRAAMRAGAGMVRLGVPGAEGRDLPAGEVVAQPLPAARWERTVLDALKRCRALAVG
                     PGLGRSEEALDAVRAVVTAAERPVVVDADGLAALGERAAAAAVLAPRPLPAVVTPHEG
                     EYAVLARSPVGPQRLADVRRLARDLGAVVLLKGSTTVISDPGGAAFFVTAGGPRLATA
                     GTGDVLTGILGAFLARGVPPLLAAGLAAHAHGSAAALGPAEGLVAGDLLDLLPRYLSA
                     AGGG"
     gene            complement(2590..3024)
                     /locus_tag="VF954_04430"
     CDS             complement(2590..3024)
                     /locus_tag="VF954_04430"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX9530362.1"
                     /translation="MTGPGEGRVPGPGEGRVPGEGRVLGLGIDVVDLDRFRLAIGRTP
                     GLVERVFTPGERAYALRKRDPAERLAARFAAKEATMKALGVGLGAFRFHDVEVTKARS
                     GAPSLALTGAAAALAERRGVGAWHIALTHSALIAEAVVLALG"
     gene            complement(3021..>6312)
                     /locus_tag="VF954_04435"
     CDS             complement(3021..>6312)
                     /locus_tag="VF954_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX9530363.1"
                     /translation="LVRHPALAPQVRARIDEVEGWVAAREAGLDAQPTLTGAPTLTGA
                     PTLTGANTLTGGPTLTGAALEDANAALLRLKDAAWAVSRDRLRVAAAVAELLGGHGES
                     PARAAPEAVDAFLAIEVALSALDRLEVRGRDSAGLHVMVHGTGLDLAGADADLLRGRD
                     DPLFTSMAARAPDGCLSVVYKAAAEIGELGDNVRALRAAITADPLLHRAVASPGAWAT
                     VLAHTRWASVGVISEANAHPLNQEEADGDGPYVVAALNGDVDNYAELAARDGLTLPAE
                     LTTDAKVIPALVSRRMAQGASGLEAFRATVGEFHGSVAIGANTAGSAGEVLLALKGSG
                     QALYVGLAEDAFVVASEPYGLVEQTSSYVRMDGETTGGQIVVLDAEKAGALAGVARYR
                     YDGTPDPIGEEEVQTAEITTRDIDRRGYPHYLLKEISEAPSSLAKTLRGKVVAGSDGL
                     WEVRLGEEAVPEGVRSRLAEGAIRRIVVIGQGTAAVAGHSVAAALRTALSGAAIAVSA
                     LPATELSGFGLADDMSDTLVVAISQSGTTTDTNRTVDLVRSRGATVLAVVNRRNSDLV
                     EKSDGVLYTSDGRDVEMAVPSTKAFYAQVAAGFLLALAVADAAGLAERHSASAVLAGL
                     RALPAAMTRVLETRDEIAALAHRHAPARRSWAVVGNGPNRVAAEEVRIKLSELCYKSI
                     ACDVTEDKKHIDLAAEPLILVCAAGLTGANADDVAKEIGIYRAHKAFPVVIATEGVEA
                     LDIPGHAITVPDVAPEVAFVLSAMAGHLFGYEAALSIDAQARPLRQARAAIEGALPAL
                     DAGEDPFPRLAPVLAPLARAFLDGVRAGAYNGHLEASTALRLGNLLRFTTGQTPIEGY
                     EAEIGAPATPGAFLADVTDALSAAIEELTRPIDAIKHQAKTVTVGISRSEDALFGSPL
                     VARVLAVGARRDRLPYRVVRTLAAIDPAVTEVLGYTRYRIEGDVGLGTASIGVLDKGG
                     IAAGIPSRAEHDPTLRGTKHRAAKEQEVILAVGRQDGRPVLLVPEVKDQHVVGLALLH
                     IRFADRLPAATARSVLAGYRKDRYAALVDTVTETEPTFDESRLGEVAVVDLLLQPVVQ
                     LADRWRRA"
BASE COUNT          774 a         2373 c         2444 g          721 t
ORIGIN      
        1 ccgggaagag caccaccgtg cagcccgtcg cggcggcccc gtcggtccag tgcccgaccc
       61 gaaccccggc cacgtcggtg atcacgggcc gccgacgtag acgcggggga cgcgggggcc
      121 gatgccgcag acgatctcgt agctgatggt gtcgaggcgc gaggcccagt cctcggcggt
      181 gatgcgcagc ccctgctggt cgccgaggag caccacctcg tcacccgcgg cgacgccgcc
      241 gccgggaccg cagtcgacca tgagctggtc catggtgatg gtgccggcga ccggccggcg
      301 ccggccgcgg atgagcacgt gggcgcccgt ggccgacagc cggcggggca cgccgtcggc
      361 gtagccgagg ggcaccgtgg ccaccaccga gtcggcgtcc agccggtacc gcaggccgta
      421 ggagacccgg tcgccggcgg cgaccccgcg ggccagcgtg acccgggcct tgagcgcgag
      481 cgcgggccgc aggcgcagcc cgccgtccag ccccggggcc ggggcgtagc cgtacagccc
      541 gatgccgcaa cgcaccaggt cgccccggga ccgggggtgg agcagggccc cggcggagtt
      601 ggcggcgtgg acgagcgggg gccggatgcc gagggcctcg aggtcggctc gcaccccctc
      661 caggacctgg agctggtgct cggtgaagcc gtcctcgggc tcgtcggcca ccgccagatg
      721 ggtccagacc ccggccaggc gcagcacccc ggaggagatc acggcccgga tggcctcgac
      781 ggcgtgggcc ggcgaggccc ccacccggtg catgccggtg tcgatcttga agtgcacgtc
      841 cagcgtgcgg tcgctggccc gggcggcccg ctcgagggcc gccgccgccg gccggctgta
      901 cagggtgggg gtcaggtcgc aggccaccac ctcgtccatg gcgtcgggca cgggctcgga
      961 gagcaggagg atgggggcgc cgatcccggc gtcgcgcagc tcccgtccct cctcgaccag
     1021 ggcgacggcc agccaggtgg ccccgccggc gagcgccgcc cgggcggcgg gcacggcgcc
     1081 gtggccgtag ccgttggcct tgaccaccgc gcacagctgc gccggggcgg ccgcggcggc
     1141 cagggcggac gcgttggccc ggatggcacc gaggtcgacc tccgcccacg ccgggcggaa
     1201 gtggcgggcc ggctcaccca ccgccggccg ccgacaggta gcggggcagc aggtccagca
     1261 ggtcgccggc gacgagcccc tcggccggcc cgagcgcggc cgccgacccg tgggcgtggg
     1321 cagccagccc ggcggccagc aacggcggca cgccccgcgc caggaaggcc ccgaggatgc
     1381 cggtcagcac gtcgcccgtg cccgcggtgg ccagccgggg tccgccggcg gtcacgaaga
     1441 aggcggcccc gccgggatcg gagatcacgg tggtggagcc cttcagcagc accaccgcac
     1501 ccaggtcgcg ggccaggcgc cgcacatcgg ccagccgttg gggtccgacg ggcgagcggg
     1561 cgagcacggc gtactcgcct tcgtggggcg tcaccacggc cgggaggggc cgcggcgcca
     1621 gcaccgccgc ggcggccgcc ctttctccca gagcggccag gccgtcggcg tccacgacca
     1681 cggggcgctc ggcggccgtg accacggctc gcaccgcgtc cagtgcctcc tccgaccggc
     1741 ccaggcccgg gcccaccgcc agcgcccggc accgcttcag ggcgtcgagg acggtgcgct
     1801 cccaccgcgc cgccggaagg ggctgggcca cgacctcgcc cgccggaagg tcgcgtccct
     1861 cggcgccggg gacgcccagg cggaccatcc ccgctccggc ccgcatggcc gctcgactgc
     1921 acagccgggc cgcccccatc atgccggggg aaccggcgac cacggccagc gccgtctgcc
     1981 atttgtgggc ctcgggcggg cgggtcggca gcgcggccag gtcggcgtcc tccagccggt
     2041 gggcggtggc gtccgaggcg tccaggccga tgtccgccac gcggacctgg ccggcccggg
     2101 cccggccctc gccgagcagc agccccggct tgagggccac gaaggtcacc gtcacgtcgg
     2161 cccgcaccgc ttcgggggac gcctcgccgg tgtctcccat gacacccgac gggatgtcga
     2221 ccgccaggac gggcgcgctc gccacggggg cgcggtactc gccggcgaac ccagtgccgt
     2281 atgcggcgtc gaccacgagg tcgcacggcg gcagggagcg gggcgcctcc cgggcgtcga
     2341 cgagcaggac gcgggcgccg gccgccgtca gccggtgggc ggccacccgg ccgtcgtcgc
     2401 cgttgtggcc cttgcccgcg accaccacca cccgccgccc gtaggtgccg cccagcatcc
     2461 ggcgggcctc ccgggccacc gcccggccgg cgcgctcgat caaggcctcg acggccaccg
     2521 accgccgggc gagggcgtcc acctcggcca tctcggcgac ggtgaggacc ggctgcacga
     2581 cccgcgccct cagcccagcg ccaggacgac ggcctcggcg atgagggcgg agtgggtgag
     2641 ggcgatgtgc cacgcgccga cgccgcgccg ctcggccagc gcggcggccg cgccggtgag
     2701 ggcgagcgac ggcgcgcccg agcgcgcctt ggtgacctcg acgtcgtgga acctgaacgc
     2761 ccccagcccc acccccagcg ccttcatggt ggcctccttg gccgcgaacc tggcggccag
     2821 gcgctcggcc gggtcgcgct tgcgcagggc gtaggcccgc tcgcccggtg tgaagacgcg
     2881 ctcgaccagg cccggcgtcc gcccgatggc gagccggaag cggtccaggt cgacgacgtc
     2941 gatcccgagg cccagcaccc gtccctcgcc cggcacccgt ccctcgcccg ggcccggcac
     3001 ccgtccctcg ccgggccctg tcacgcccgg cgccaccggt cggcgagctg cacgaccggc
     3061 tggagcagga ggtcgaccac ggccacctcc cccagccgcg actcgtcgaa cgtcggctcg
     3121 gtctcggtca cggtgtccac cagggcggcg tagcggtcct tgcggtaccc ggcgaggacc
     3181 gagcgggccg tggccgcagg cagccggtcg gcgaaccgga tgtgcagcag ggccaggccg
     3241 accacgtgct ggtccttgac ctcgggcacc agcagcaccg gccggccgtc ctggcggccg
     3301 accgccagga tgacctcctg ctccttggcg gcccggtgct tggtgccgcg cagggtggga
     3361 tcgtgctcgg cccgcgacgg gatgccggcg gcgatgcccc ccttgtcgag cacgccgatg
     3421 ctcgccgtgc ccagcccgac gtcgccctcg atccggtagc gggtgtagcc gagcacctcg
     3481 gtcacggccg ggtcgatggc ggccagggtc cgcaccacgc ggtacggcag ccggtcgcgc
     3541 cgggcgccca cggcgagcac gcgggccacg agcggggagc cgaacagggc gtcctccgac
     3601 cgggatatgc cgacggtgac ggtcttggcc tggtgcttga tggcgtcgat gggccgggtc
     3661 agctcctcga tggccgcgga gagggcgtcg gtcacgtccg cgaggaacgc gccgggggtg
     3721 gccggagcac cgatctcggc ctcgtagccc tcgatcgggg tctggcccgt ggtgaagcgc
     3781 agcaggttcc cgagccggag ggcggtggac gcctccaggt ggccgttgta ggcaccggcc
     3841 cggacgccgt cgaggaaggc ccgggccagg ggcgcgagca cgggggccag gcgcgggaag
     3901 gggtcctcgc cggcgtccag cgccgggagg gcgccctcga tggcagcccg ggcctggcgg
     3961 aggggccggg cctgggcgtc gatggagagc gcggcctcgt agccgaagag atgaccggcc
     4021 atggccgaca gcacgaaggc cacctcgggg gccacgtcgg gcacggtgat ggcgtggcca
     4081 gggatgtcga gcgcctcgac tccctcggtg gcgatcacca cggggaaggc cttgtgggcc
     4141 cggtagatgc cgatctcctt ggccacgtcg tcggcgttgg cccccgtgag cccggccgcg
     4201 cagaccagga tgagcggctc ggcggccagg tcgatgtgct tcttgtcctc ggtgacgtcg
     4261 caggcgatgg acttgtagca cagctccgac agcttgatgc gcacctcctc ggccgccacc
     4321 cggttgggcc cgttgccgac gacggcccag gagcgccggg cgggcgcgtg ccggtgggcg
     4381 agggcggcga tctcgtcacg ggtctcgagc acgcgggtca tcgccgccgg cagggcgcgc
     4441 aggccggcca gcacggcgga ggcgctgtgc cgttcggcga gcccggccgc gtcggccacc
     4501 gccagcgcga gcaggaaccc cgcggccacc tgggcgtaga acgccttggt cgagggcacc
     4561 gccatctcca cgtcccgccc gtcggaggtg tagaggacgc cgtcggactt ctccaccagg
     4621 tcgctgttgc ggcggttgac gacggccagc acggtcgccc cccggctgcg caccaggtcg
     4681 accgtgcggt tggtgtccgt cgtggtcccc gactggctga tggccaccac cagcgtgtcg
     4741 ctcatgtcgt cggccaggcc gaagccggac agctcggtgg cgggcagagc cgagacagcg
     4801 atggctgcgc cgctgagcgc ggtgcgcagg gcggcggcga cgctgtggcc ggccaccgcc
     4861 gcggtgccct ggccgatgac gacgatgcgc cggatcgcgc cctcggccag ccgggagcgc
     4921 acaccctcgg ggacggcctc ctccccgagg cgcacctccc acaggccgtc ggagccggcc
     4981 accaccttgc cccggagggt cttggcgagc gaggacggcg cctccgagat ctccttgagc
     5041 aggtaatggg ggtagccccg ccgatcgatg tcgcgggtcg tgatctcggc ggtctgcacc
     5101 tcctcctcgc cgatcgggtc gggcgtcccg tcgtagcggt accgcgccac cccggccagg
     5161 gcgcccgcct tctcggcgtc cagcacgacg atctgcccgc cggtggtctc cccgtccatc
     5221 cgcacgtacg acgaggtctg ctccaccagg ccgtagggct cgctggcgac cacgaaggcg
     5281 tcctcggcca ggccgacgta gagcgcctgg ccgctgccct tgagggcgag cagcacctcg
     5341 ccggcggagc ccgccgtgtt ggccccgatg gccaccgacc cgtggaactc gcccaccgtc
     5401 gcccggaacg cctccaaccc gctcgcgccc tgcgccatcc ggcgcgacac cagcgccggg
     5461 atgaccttgg cgtcggtggt gagctcggcg ggcagcgtga gcccgtcgcg ggcggccagc
     5521 tcggcgtagt tgtccacgtc gccgttgagg gcggccacga cgtagggccc gtccccgtcg
     5581 gcctcctcct ggttgagggg gtgggcgttg gcctcggaga tgaccccgac gctggcccac
     5641 cgggtatggg ccaggaccgt ggcccaggcc ccgggactgg caacggcccg gtgaagcagc
     5701 gggtcggccg tgatggcggc gcgcagggcg cggacgttgt cgccgagctc gccgatctcg
     5761 gctgcggcct tgtacaccac gctgaggcag ccgtcggggg cccgggccgc catggaggtg
     5821 aagagcgggt cgtcccggcc tcgcaacaga tccgcgtcgg caccggcgag gtcgagaccg
     5881 gttccgtgga ccatgacgtg caagccggcc gagtcccggc ccctgacctc gaggcggtcg
     5941 agggcggaaa gggcgacctc gatggccagg aaggcgtcga ccgcctcagg cgcggcgcgg
     6001 gccgggcttt cgccatgccc gccgagcagc tcggcgacgg ctgccgcgac ccggaggcgg
     6061 tcgcggctga ccgcccacgc cgcgtccttg agcctgagga gggcggcgtt ggcgtcctcc
     6121 agggcggcgc cggtcagggt ggggccgccg gtcaaggtgt tggcgccggt cagggtgggg
     6181 gcgccggtca gggtgggggc gccggtcagg gtgggctggg catccaggcc cgcctcccgg
     6241 gctgcgaccc agccttcgac ctcgtcgatg cgggcgcgga cctggggcgc cagcgcgggg
     6301 tgccgcacca gg
//
LOCUS       DASWGG010000245         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3130327, whole genome shotgun sequence.
ACCESSION   DASWGG010000245 DASWGG010000000
VERSION     DASWGG010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3130327"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..2575)
                     /locus_tag="VF954_04440"
     CDS             complement(176..2575)
                     /locus_tag="VF954_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530364.1"
                     /translation="MTGSAIAGRLLALWTAYGVPCLEALGLAIVGVLAGAMLRVSRRR
                     RRIAAMATTGSWIRVTPPPEVDAAGARDFWAALGELRRRGEPWVVWHFQATGGAGAGV
                     WLWVPQVIPVENIRRAVQGAWPKARTEESAPPPVEAPKLPSRREMAAERDRYRRAQAD
                     GETPEEPKTPPARLVGAELALRSSAMHALDTDSAESLHRHLIAQVQHLEVGEAVVVQV
                     LARPAPGKSYGRLLRHAGQAEAEAHRLRSLNAPGATRAMAQVRKAWPKLDDALWLTRV
                     RVAVLGNHPGRATGRIDGVLSAFGVVRAPAQHLTRRRCTVVPGDLRHDRGDLLSAGEL
                     ATLAGLPSASILEASRARVVAAGPEVPSKGMRLGISVGRPVAIAFPDVLYHAHVIGPT
                     GVGKTTLLTNMALDLLRANWSLVTADIAKGDLVDWILARLPESMVGKVCVIDPTAESV
                     PGLNVLATDNPVLTADQLVGTFSRLYSSAWGPHLEQVLRQAFLTLAYGGGTLVELELL
                     LTDESFRSKMVGRVLADAGRRRSSYAMAKLEGYWQEFDGKSDSERGRLLDPVLYKLDG
                     FLPASVAAVIGQVNPKGDPLSLIEEGGLVVVRAPAGEVGDDPARLLASLLAIRNWQRL
                     QARSAVPEDERPRLQRVAFLADEAQDMFGSRAKGQGEVAERMLVQARGLGGAFIVGHQ
                     HLAQLTPDFAKALGSNATTKVLFRLDEDDAKKFAPRVSPEFREQDLMALEFGQAVCKP
                     CINRGQGAAFTFRTEPLPPGDQRRLEAATAASAQRWGRPRAEVEEEMVARHRGHLQVV
                     R"
     gene            complement(2576..>2813)
                     /locus_tag="VF954_04445"
     CDS             complement(2576..>2813)
                     /locus_tag="VF954_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530365.1"
                     /translation="DPEVTYDTSGCHPPLDRGDRGLLVDPDTNARAAWRLASSGRGGF
                     DDWSTYTNGAYSAWLPQAAAAAAAYATATATGGT"
BASE COUNT          398 a         1012 c         1027 g          376 t
ORIGIN      
        1 gggtcaggcc ggttgcagcg gaccgagcca cgcacaaatc gggggttgca gtgaccgcct
       61 ccccactttg gcagaatctc cagtcctccg tgaccgccga ccaccccacc gaccacccca
      121 ccgaccaggc tgcatgtaga cgtccacccg ttcactcgta cacgtgaacg agtcgtcatc
      181 gcaccacctg gaggtggccg cggtggcggg ccaccatctc ctcctccacc tcggcccggg
      241 gacggcccca gcgctgtgca gacgctgccg tggcagcctc gagacggcgc tggtcgccgg
      301 ggggcagggg ctcggtgcgg aaggtgaacg cagcaccctg gccccggttg atacacggct
      361 tgcagaccgc ctggccgaac tccagcgcca tcaggtcctg ctcccggaac tcgggcgaca
      421 cccggggggc gaacttcttg gcgtcgtcct cgtccaggcg gaacaggacc ttggtggtgg
      481 cgttggaccc gagcgccttg gcgaagtccg gcgtgagctg ggcgaggtgc tggtggccga
      541 cgatgaacgc cccgcccagc ccccgggcct gcacgagcat ccgctcggcg acctcgccct
      601 ggcccttggc ccgggagccg aacatgtcct gcgcctcgtc cgccaagaac gcgacccgct
      661 gcagcctggg ccgctcgtcc tcggggacgg cggagcgggc ctggaggcgc tgccagttgc
      721 ggatcgccag gagcgacgcg aggagccggg ccggatcgtc gcccacctcg ccggccgggg
      781 cccgtacgac caccaagccg ccctcttcga tcagggacag gggatcgccc ttggggttga
      841 cctgcccgat gacggcggcg accgaagccg gcaggaaccc gtccaacttg tagagcacgg
      901 ggtccagcag gcggccgcgc tccgagtccg acttgccgtc gaactcctgc cagtacccct
      961 cgagcttggc catggcgtac gagctgcgcc ggcggcccgc atcggccagg acgcggccga
     1021 ccatcttgga gcggaaggat tcgtcggtca ggagcagctc gagctcgacg agcgtgccgc
     1081 caccataggc cagggtcagg aacgcctggc gcagcacctg ctcgaggtgc gggccccatg
     1141 ctgacgagta gaggcggctg aaggtgccga cgagctggtc agccgtgagc accgggttgt
     1201 cggtggcgag cacgttcagg cccggcacgg actccgccgt cgggtcgatc acgcagactt
     1261 tgcccaccat cgactcgggg agtcgggcga ggatccagtc caccaggtcc cccttggcga
     1321 tgtcggcggt gaccagggac cagttagccc tgagcaaatc gagggccatg ttggtcagca
     1381 gggtggtctt gccgacgccg gtggggccaa tgacgtgggc gtggtagagc acgtccggga
     1441 aggcgatcgc cacggggcgg ccgaccgaga tccccagccg catgcccttg ctcggcacct
     1501 ccggcccggc cgccacgacc cgggcgcggc tcgcctccag gatcgacgcc gagggcaggc
     1561 ccgccagggt ggccagctca ccggccgaca gcaggtcgcc ccggtcgtgg cggaggtcgc
     1621 ccggcaccac ggtgcagcgg cggcgggtga ggtgctgggc cggggcgcgg accacgccga
     1681 aggcggacag gaccccgtcg atccgccccg tggcgcgccc ggggtggttg ccgagcaccg
     1741 ccacccgcac ccgcgtcagc cacagggcat cgtcaagttt cggccaggcc ttgcgaacct
     1801 gcgccatcgc cctggtggcc ccgggggcat tgagggagcg gaggcggtgc gcctcggcct
     1861 cagcctgccc ggcgtggcgc agcaggcgtc cgtaggactt ccccggggcg gggcgggcga
     1921 ggacctggac gacgaccgcc tccccaacct cgaggtgctg gacctgcgcg atcaggtgcc
     1981 ggtgcagcga ctcggcgctg tcggtgtcga gggcgtgcat ggccgaggac cgcagcgcca
     2041 gctcggcgcc gacgaggcgg gccggcggcg tcttgggttc ctcgggggtc tcaccgtcgg
     2101 cctgggcgcg ccggtaccgg tcacgctcgg cggccatctc ccggcgcgag ggcagcttcg
     2161 gggcctcgac gggtggcggt gcggactcct cggtacgggc cttcggccat gcgccctgga
     2221 ctgctcggcg gatgttctcc acggggatga cctgggggac ccagagccac acgccggcgc
     2281 cggctccgcc cgtggcctgg aagtgccaca cgacccaggg ctcccccctg cggcgcagct
     2341 cgccgagggc ggcccagaaa tcccgggctc cggcggcgtc gacctccgga ggcggcgtca
     2401 cccggatcca gcttccggtg gtggccatgg cagcgatgcg ccggcgccgg cgtgataccc
     2461 ggagcatcgc tcccgccagc acccccacga tcgccaggcc cagcgcctcg aggcagggca
     2521 ccccgtacgc ggtccacagt gccaagagac gccctgcgat tgccgacccg gtcatctacg
     2581 ttcctccggt cgcggtggct gtggcatagg cggccgcagc ggctgcggcc tggggcagcc
     2641 aggccgagta agcgccgttg gtgtaggtgc tccagtcgtc gaaaccaccg cggccgctcg
     2701 aggcgagccg ccaggcggcc cgtgcgttgg tgtcggggtc gacgagcagg ccacgatcgc
     2761 cccggtccag cggcggatga cagccgctcg tgtcgtaggt cacctcgggg tcc
//
LOCUS       DASWGG010000246         3967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2798428, whole genome shotgun sequence.
ACCESSION   DASWGG010000246 DASWGG010000000
VERSION     DASWGG010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2798428"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2012)
                     /locus_tag="VF954_04450"
     CDS             complement(<1..2012)
                     /locus_tag="VF954_04450"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX9530366.1"
                     /translation="MAARRFGAPQSTRRLFATFALITLLPVLLLGAVLAASYRREARR
                     RGVAEGRQEASLVARAAVEPLLAGHDLGAGLSPDEGAALRRLVGRTTSGHDVLRLRLE
                     DLSGRVVYSEDGSGFADKPEDEALQAAHGEIVTRLTRLNADSNDRGPKGVDAVEAYRP
                     LYGGSPARQVGVLEVYLPYAPISHDVTDGLNSLYRDLALGLAALYLAVFTVSALLSRG
                     LRRQLALNAFLAEHDALTELPNRTLFHRRAEEAVSGATAGAQNVAIAIIDLDRFREVN
                     DTLGHHNGDRLLTELGLRLAAFTQRGNTVARLGGDEFGVILRDVADPDAALRQLRNVI
                     ELEVEVSGVPLTVEASVGYVLAPDDGEDIDELLQHADMAMYVAKAGHTGVARYDRRQD
                     HYDPANLGLIAELRHAIDDGQLVLHYQPKVALGDGHVEAVEALVRWQHPSLGLLAPDR
                     FIPLAEQTDLIDKLTYWVVESALADLQDLGARAAGLSVAVNVSARNLARPDFASRVVD
                     ILDRDGVPAGRLIVEITETALLVDPARAATILADLANAGARVSIDDFGRGQTSLGYLS
                     ELPVHELKIDRIFVADMLVNPAHAAIVRSIVDLGHNLSLRVVAEGVETDAVLSALRDT
                     GCDVAQGFLLARPMAVGALGEWLRGPGSHGGHLSAGWTSAGSAPATV"
     gene            complement(2086..2382)
                     /locus_tag="VF954_04455"
     CDS             complement(2086..2382)
                     /locus_tag="VF954_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530367.1"
                     /translation="MPFIQTIEYQTTRIDELNTLVDGWLEKTKGKRAATRGTQTRDRD
                     AANTYMQIVEFPSYEDAMLNSKLPETSELAAQMAALCDGPPKFRNLDVTRADQM"
     gene            complement(2443..3243)
                     /locus_tag="VF954_04460"
     CDS             complement(2443..3243)
                     /locus_tag="VF954_04460"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX9530368.1"
                     /translation="MTIEDRPTVDAPAPTTDLDQARRDLEATGLCVVADVLTGDALTE
                     VRDAAYRAAASDRRRGRVQRFGGDFDVTNQRVWNLPSRDPVFLDVVEHPLALSFVREV
                     IGWPALLSNISANLTGPGGGEMYFHCDQFYMPQPWSGVQGINMIWCVDDFTEDNGATR
                     VVPGSHALNRPPLQDDRLVATVPLEARRGSMVVMDGRVWHRTGTNRTRDEHRCGLFAW
                     YTPPIYLPQENWWLSLDPSVRLFGSDELLTLFGFKATSFGRVYDRSPA"
     gene            complement(3240..3908)
                     /locus_tag="VF954_04465"
     CDS             complement(3240..3908)
                     /locus_tag="VF954_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9530369.1"
                     /translation="MTERRPRLRGRPTNGSSEQTRRRILDGARRVFSSQGYSRATMRA
                     IAAEADVTAKAVYKYSPSKAALFVAVWEDAIEQIYADYEHVVAAQGSLLGEVEALLDR
                     SRQLLVERPDHTRLSLRVLLDHEHPDLVGADLQPRAALEFYRNLAARGVQRGEITDLN
                     REHVVVFIVTLLWGIMTLGAFDPATLDQSVAAAKWAARRQLQPVTTPIEALTREALTP
                     GGRT"
BASE COUNT          550 a         1328 c         1484 g          605 t
ORIGIN      
        1 accgtcgcag gcgcgctgcc ggcgctggtc catccggcag aaaggtggcc cccgtgcgaa
       61 ccggggccgc gcaaccattc gcccagcgcg cccacggcca tgggccgggc gagcaggaag
      121 ccctgggcca cgtcgcagcc cgtgtcgcgg agcgcgctga gcacggcgtc ggtctccacg
      181 ccctccgcca cgacgcgcag ggacaggttg tggccgaggt cgacgatgga acggacgatg
      241 gccgcatgcg ccgggttcac cagcatgtcg gccacgaaga tcctgtcgat cttgagctcg
      301 tggaccggca gctcggagag gtagcccagg gaggtctgtc cccggccgaa gtcgtcgatg
      361 ctcacgcggg cgcctgcgtt ggccaggtcg gcgaggatgg tcgcggcccg tgccgggtcc
      421 acgagcaggg cggtctcggt gatctccacg atgaggcggc cggccggcac cccgtcacgg
      481 tcgagtatgt cgaccaccct ggacgcgaag tcagggcggg ccaggttgcg ggcggagacg
      541 ttgaccgcca ccgacaggcc ggccgctcgc gcccccaggt cctgcaggtc ggcgagagcg
      601 gactcgacga cccagtaggt gagcttgtcg atcaggtcgg tctgttcggc gagcgggatg
      661 aagcggtcgg gggcgagcag accgagactg ggatgttgcc agcgcaccag ggcctcgacg
      721 gcctcgacat ggccgtcgcc gagggccacc ttcggctggt agtgcaacac cagctggccg
      781 tcgtcgatgg cgtggcgcag ctcggctatg agcccgaggt tggccgggtc gtagtggtcc
      841 tgtcggcggt cgtagcgcgc gacgcccgtg tgcccggcct tggcgacgta catggccatg
      901 tcggcgtgct ggagcagctc gtcgatgtcc tcgccgtcgt ccggcgcgag gacgtagccc
      961 acgctggcct cgacggtcag gggcacgccg ctgacctcga cctcgagctc gatgacgttg
     1021 cggagctggc gcagcgccgc atccggatcc gcgacgtcgc gcaggatcac gccgaactcg
     1081 tcgccgccca ggcgcgccac cgtgttgcct cgctgggtga acgcggccag gcggaggccc
     1141 agctcggtga gcaaccggtc cccgttgtgg tggccgagag tgtcgttgac ctcccggaag
     1201 cgatcgagat cgatgatggc gatggccacg ttctgggcgc ccgcggtcgc gcccgacacg
     1261 gcctcctcgg cacgccgatg gaacagcgtc cggttgggta gctcggtcag ggcgtcgtgc
     1321 tcggccagga aggcgttcaa ggcgagctgg cggcgcagcc cccggctcag gagagcggac
     1381 accgtgaaca cggcgaggta cagcgcggcc agacccaggg ccaggtcgcg gtacagactg
     1441 ttgagcccgt ccgtcacgtc gtggctgatg ggcgcgtagg gcagatagac ctcgaggact
     1501 ccgacctggc gggccgggct cccgccgtac aacggtcggt aggcctcgac cgcgtccacg
     1561 cccttggggc cgcggtcgtt gctgtcggcg ttcagccggg tgagccgcgt cacgatttcg
     1621 ccgtgagcgg cctgcagggc ctcgtcctcc ggtttgtcgg cgaagcccga tccgtcttcg
     1681 gagtacacca cccggccgct gagatcctcc agccggagcc ggagcacgtc atgtccggac
     1741 gtggtccgac ccaccagccg ccgcagggcc gcgccctcgt cggggctcag cccggcgccc
     1801 aggtcatgcc cggccaggag cggctcgacc gccgcccggg cgaccagcga ggcctcttgt
     1861 cggccctcgg ccaccccgcg acgccgggct tcgcgacggt agctggcggc cagcacggcg
     1921 ccgagcagca ggaccggcag cagggtgatc agcgcgaagg tcgcgaacag ccgccgggtc
     1981 gactggggcg cgccgaaccg tcgagctgcc atccctcatg ttcggcgcgt cccgggcccg
     2041 gcttgacccg cctggcctgc gactaccggg ccgccaccgc gtttgctaca tctggtcggc
     2101 ccgggtgacg tcgaggttgc ggaactttgg cggcccgtcg cacagcgcgg ccatctgggc
     2161 ggccagctcg ctggtctcgg gcagcttcga gttgagcatg gcgtcctcgt aggaggggaa
     2221 ctcgacgatc tgcatgtagg tgttggccgc gtcccggtcc cgggtctgtg tgccccgggt
     2281 ggcggcgcgc ttgcctttgg tcttctccag ccagccgtcc acgagcgtgt tcagctcgtc
     2341 gatgcgggtg gtctggtact cgatggtctg gatgaagggc atcgcgctcc ccctggtcaa
     2401 ggccatgacg tcatggccgg gcgcgatcat aggccgaacc ggtcaggcag gcgatcggtc
     2461 gtatacgcgg ccgaaggacg tggccttgaa gccgaagagg gtgagcagct cgtcggagcc
     2521 gaagaggcgc acgctcgggt cgagggagag ccaccagttc tcctggggca ggtagatcgg
     2581 cggcgtgtac caggcgaaca ggccgcaccg gtgctcgtcg cgggtgcggt tggtgccggt
     2641 gcggtgccag acccggccgt ccatgaccac catcgagccc cgccgggcct cgagcggcac
     2701 cgtcgccacc agccggtcgt cctggagcgg aggccggttg agggcgtggc tgccgggcac
     2761 gacccgggtg gcgccgttgt cctcggtgaa gtcgtcgacg caccagatca tgttgatgcc
     2821 ctggacgccg ctccatggtt gcggcatgta gaactggtcg cagtggaagt acatctcccc
     2881 gccgccgggc ccggtgaggt tggccgagat gttggacagc agcgccggcc agccgatcac
     2941 ctcgcggacg aagctcagcg ctagcgggtg ctcgacgacg tcgaggaaga ccgggtcgcg
     3001 gctggggagg ttccagaccc gctggttggt gacgtcgaag tcgcccccga agcgctgcac
     3061 gcggccgcga cggcgatctg atgccgcggc gcggtaggcc gcgtcgcgca cctcggtgag
     3121 cgcgtcgccg gtgaggacgt cggccaccac gcagagcccg gtggcctcca ggtcgcgccg
     3181 ggcctgatcg aggtcggtgg tcggggccgg ggcgtcgacg gtggggcggt cctcgatggt
     3241 catgtgcgtc ctcctggtgt cagcgcttct cgtgtcaggg cttcgatcgg cgtggtcacc
     3301 ggctggagct gccgccgggc tgcccatttg gccgccgcca cgctctggtc gagggtcgcc
     3361 gggtcgaagg cgccgagcgt catgatcccc cacagcaacg tgacgatgaa caccacgacg
     3421 tgctcgcggt tgagatctgt gatttccccc cgttgcaccc ctcgggccgc caggttcctg
     3481 tagaactcga gagccgcgcg cggctggagg tcggcgccca cgagatcggg gtgctcgtgg
     3541 tcgagcagca cccgcaggga gaggcgggtg tggtcggggc gctcgaccag cagctggcgc
     3601 gaccggtcca ggagcgcctc gacctcgccg agcaacgagc cctgggcggc cacgacgtgc
     3661 tcgtagtcgg cgtagatctg ctcgatggcg tcctcccaca ccgccacgaa cagggcggcc
     3721 ttcgacgggg agtacttgta gacggccttg gccgtgacgt ccgcttcagc ggcgatggcg
     3781 cgcatggtgg cgcgggagta cccctgcgag gagaagaccc gccgggcgcc gtcgaggatg
     3841 cgacggcggg tctgctcgct gctgccgttg gtcggtcgac cgcggagcct cggtcgcctc
     3901 tcggtcacgg tgagccgacc ccggccgcgc taagcggtga ccgcggtggg tcgaacggtg
     3961 accttcg
//
LOCUS       DASWGG010000247         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2800019, whole genome shotgun sequence.
ACCESSION   DASWGG010000247 DASWGG010000000
VERSION     DASWGG010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2800019"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VF954_04470"
     CDS             complement(<1..378)
                     /locus_tag="VF954_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9530370.1"
                     /translation="MAIAITEAHRDLESVARSFLSRAGARAAARALLDAPDEALPAFW
                     ADLVELGWLGLHLPEELGGSGYGLEELVVVIQELGRALAPGPFLPTVLASATIRLAAS
                     KEQQAMLLPGLADGSTTAALGLDG"
     gene            539..1636
                     /locus_tag="VF954_04475"
     CDS             539..1636
                     /locus_tag="VF954_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9530371.1"
                     /translation="MTTAARIAVLPAGPGPLRIDDVVLPDPGPRQVVVRQFASGVCHS
                     QLHQMHGPRQAPTVLGHESTGVVLAAGREVSHVGEGDRVMVTWVPRRPEEAGRSPESA
                     RLPLPDGTTAVSQNVFTWADHTIADEQFVVALPDDVKTDVTSIIGCAVMTGAGAVRNT
                     AGVRRGESVAVFGVGGVGLSAVVAARVVGADPIIAVDLRDDKLEMARRFGATETVNAS
                     AGDAVARVRELTADPNLVDMLGRPVGGVDYAFDCIGVKATMEQIVPAARSGVLGAARG
                     GAGVLVGVPRGSVELNAIDMLVNEKRFVASLGGSCQPERDFPVFLDWYRDGRLDLDAL
                     VTARYALEDINEATTDLEEGRIFGRSILVFD"
     gene            complement(1662..2066)
                     /locus_tag="VF954_04480"
     CDS             complement(1662..2066)
                     /locus_tag="VF954_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX9530372.1"
                     /translation="MSFSVHRDDFSAPFFDAAASGSLLIRRCPRCGRHYPPQQRRCPD
                     GEEAEWAPAAGTGVLVTWGVEHGPPPDPDLAGPDGATSVFGYVELDEGPWYPAAIVDA
                     DPASLAAGDQMAVKFLRLGDGEPVPVFAPPAS"
     gene            complement(2078..>2554)
                     /locus_tag="VF954_04485"
     CDS             complement(2078..>2554)
                     /locus_tag="VF954_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9530373.1"
                     /translation="LAQPPVYPWGWAQTHPGYSMARDSEFGLTTGAAIAGPAAMRMAG
                     ITPADVDVREIYDCYTYTCLVTLEDYGFCAKGEGGELAASGALGPGGTLPTNTGGGQL
                     SGYYLWGMTPLSEAVIQARGQGGARQILRHEVVLVSGNGGTLDYHSALVLGTEPRP"
BASE COUNT          341 a          904 c          957 g          352 t
ORIGIN      
        1 cccgtcgagg ccgagggcgg cggtcgtgga gccgtcggcc aggcccggca gcagcatcgc
       61 ctgctgctcc ttggaggcgg ccaggcggat ggtggcggag gcgaggacgg tcggcaggaa
      121 cggcccaggg gccagggcgc gcccaagctc ctggatcacg acgacgagct cctcgagccc
      181 atagccgctg ccgcccagct cctcgggcag gtgcagcccc agccatccga gctccacgag
      241 gtccgcccag aaggcgggga gagcctcgtc gggggcgtcg agcagggccc tggcggcggc
      301 gcgtgcgccg gcccggctca agaacgagcg ggcgaccgat tcgaggtcgc gatgggcttc
      361 ggtgatggcg atcgccatgg gggtcccctc tcggtgctgc ggaagtgccc gttgacgaca
      421 ccctccagat cccggcggtc aggctacttg tcggtatgaa aggggagcaa ttgctgcgtc
      481 ggcctcgcgt gcccctttca tcccgcgccc gcgagggcgt tggagtacgt tccgtggcat
      541 gaccactgcc gcccggatcg ccgtgctccc tgcaggccct ggcccgctcc gcatcgacga
      601 cgtcgtgctc cccgacccgg gcccccgcca ggtcgtggtg cggcagttcg cgagcggcgt
      661 gtgccactcg cagctccacc agatgcacgg gccccgccag gcgccgacgg tcctcggcca
      721 cgagtcgacc ggcgtcgtcc tggccgccgg ccgggaggtc tcccatgtcg gcgagggaga
      781 ccgggtgatg gtcacctggg tgccccgccg cccggaagag gcgggccgat cgccggagtc
      841 agcccggttg ccgcttcccg acgggacgac ggcggtgtcg cagaacgtgt tcacctgggc
      901 cgatcacacc atcgccgacg agcagttcgt cgtggccctg ccggatgacg tgaagaccga
      961 cgtcacgtcg atcatcggct gcgcggtcat gacgggcgcg ggggccgtgc gcaacaccgc
     1021 cggtgtccgc cggggcgaga gcgtcgctgt gttcggcgtg ggcggcgtgg gcctgtcggc
     1081 cgtggtcgcg gccagggtcg tgggcgcgga cccgatcatc gccgtggatc ttcgcgacga
     1141 caagctcgag atggcccgga ggttcggcgc cacggagacg gtgaatgcgt ccgcgggcga
     1201 cgccgtggcc cgggtccgcg agctgaccgc cgaccccaac cttgtcgaca tgctcggccg
     1261 ccccgtcggc ggcgtcgact acgccttcga ctgcatcggc gtcaaggcca ccatggagca
     1321 gatcgtgccc gcggcacgct cgggcgtgct cggcgcggcg cggggaggcg cgggggtgct
     1381 cgtcggtgtc ccgcgcggct ccgtggagct caacgccatc gacatgctcg tcaacgagaa
     1441 gcggttcgtc gccagcctgg gcgggtcgtg ccagcccgag cgggatttcc cggtgttcct
     1501 ggactggtac cgggacggtc gactcgacct ggacgcgctg gtcacggccc gctacgcgct
     1561 cgaggacatc aacgaggcca ccacggacct ggaggagggg cggatcttcg gccgctcgat
     1621 cctggtcttc gattgagctc cacccgcggc gcctgcaggc gtcaggacgc cggcggcgcg
     1681 aacacgggca cgggctcccc gtcgccgagt cggaggaact tcacagccat ctggtcgccg
     1741 gcggcgagcg acgccgggtc ggcgtcgacg atcgccgccg ggtaccacgg gccctcgtcc
     1801 agctcgacgt agccgaagac gctcgtcgcc ccgtccggcc cggccaggtc gggatcgggt
     1861 ggagggccgt gctcgacacc ccacgtgacc agcacgccgg tgccggccgc cggcgcccat
     1921 tccgcctctt cgccgtcggg gcacctccgc tgctgcggcg ggtagtggcg gccgcagcgg
     1981 gggcagcgcc ggatgagcag cgagccggag gcggcggcgt cgaagaaggg cgccgagaag
     2041 tcgtcgcggt gcaccgagaa ggacacgtcc gctcccgtca gggccggggc tccgtgccga
     2101 gcacgagggc cgagtggtaa tcgagcgtcc cgccgttgcc gctcaccagg accacctcgt
     2161 gacggaggat ctggcgcgcc ccgccctggc cgcgggcctg gatgacggcc tccgacagcg
     2221 gggtcatgcc ccagaggtag tagcccgaca gctggccgcc tccggtgttg gtgggcagcg
     2281 ttccgccggg cccgagggcg ccgctggcgg ccagctcccc gccctcaccc ttggcgcaga
     2341 agccgtagtc ctccagggtg accaggcacg tgtaggtgta gcagtcgtag atctcgcgca
     2401 cgtcgacgtc cgcaggggtg atgccggcca tcctcatggc cgccgggccg gcgatcgccg
     2461 cgccggttgt gagcccgaac tcggagtcac gcgccatcga gtagcccggg tgcgtctgcg
     2521 cccaccccca cggatagacc ggaggctggg ccag
//
LOCUS       DASWGG010000248         4451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2800022, whole genome shotgun sequence.
ACCESSION   DASWGG010000248 DASWGG010000000
VERSION     DASWGG010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2800022"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VF954_04490"
     CDS             <1..198
                     /locus_tag="VF954_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530374.1"
                     /translation="ELAQGHAPYRMNGFVTVSAATGSQLETDCQSAEQAAARAHLELR
                     RLYGRQAEALTWVLPLARGMG"
     gene            226..1422
                     /locus_tag="VF954_04495"
     CDS             226..1422
                     /locus_tag="VF954_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX9530375.1"
                     /translation="MTTAQLGAAFPFIGGIPGGSGPLLGVDRWSQPFRFDVFALYRHG
                     ALTNPNVAVFGQLGRGKSSMVKSWVRRLAAAGCRVWVLDPKGEYRRLARALGATHIEL
                     RPGGGMRLNPLQHPVHGLDQAASLCAALAETGLGRPLRPVERAGLDGALATAHPSSLV
                     GVVGALLEGGAEVRPPGMGPEAWAAEGREMVLELRRMVAGDLAGMFDGPTNQGLDPGR
                     RAVVVDLSAVYDTPALAPVMVCAMAWMRSQLAGGAGGETPPTYLVLDEAWAVLAQLGA
                     ARWLRSSWKLARSMGLANVAVLHRLSDLSASGPAGSEQTRLAEGLLADTETRVLFGQS
                     PDEARVAGERLGLSPTEVAVLPRLPRGMALWSVAGRSAVIRHVLDAEDLAIVDTDHRM
                     APGAAP"
     gene            1427..3115
                     /locus_tag="VF954_04500"
     CDS             1427..3115
                     /locus_tag="VF954_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEX9530376.1"
                     /translation="MAALVAPTAAVSVVVDAWLTGKGCAWLATGRWPGLSLGGGLAFA
                     FRLVGRPGDPSGDWRAVTGGRWGPSAGPFYALLVALPAAGAALLTGAISAVSRRRPVP
                     RLGPPRPARRGSLARRRRWAPDRAGRWATVRDLRPLVVRRAAPGRVVLGRHGRGLVAG
                     EAGHSVLVVGPTQTYKTSGLVVPALLEWEGPVLAASVKSDLARVTAGWRERRGRAWVF
                     DPTATTGLPAARWSPLGEAANWAGAQRMAAWLCESGRSAMEDGDFWYATAQKLLAPLL
                     LAASVGARTMADVVRWVDEQEVAEVAGILDEAGEEDALRAAEATWRREPRQRGSVFTT
                     AETVLAAFADPAVARATESCDIAPDAVLDGGWHSLYLCAPSHEQDRMRPVFTALVQQI
                     VRRAYALSASGAAPLDPPLLVVIDEAAHVAPLADLDRTAATAAAHGIQLVTVWQDLAQ
                     VAARYGARAATVVNNHRARLVLSGVADAPTLEQISRLAGEAEVPASSVTTARDGGRST
                     TASHERRPLLAPGALRRLPPGDAVLLYGHLPPARVRLRPWFQDPALARRGQAAR"
     gene            3208..3282
                     /locus_tag="VF954_04505"
     tRNA            3208..3282
                     /locus_tag="VF954_04505"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3240..3242,aa:Lys,seq:ttt)
     gene            3378..>4451
                     /locus_tag="VF954_04510"
     CDS             3378..>4451
                     /locus_tag="VF954_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9530377.1"
                     /translation="MASIRTTPERTFEVLYRDPAGRQRSKTFKRKTDATQFSRTVEAD
                     KFRGEWMDPRLAKAPFADYAAVWWESVQLAPKTRQLYKSQLRNWVLPRFGTVPVAAID
                     APILRGWAKQITDAGRSASHRAGCINVVRLVLATAVEKGAIKVNPAYRLRLPTPSPAG
                     QMHFLSPSEIEALAHAMDDPFDALLVRFAAYTGLRAGEIYGLRVRSLSKGRVAVLDSL
                     SELQGGELVLRGTTKTGRNRVVPMPAALAEEMAALVAGRAPKDLVFAGPDGRPRSHSS
                     YYKNRFKPALAKAGLPNEVRFHDLRHTCAAMLIGLGAHPKAIMERLGHASITVTLNTY
                     GHLFPELEEALTDGLQGLWDSALP"
BASE COUNT          550 a         1598 c         1636 g          667 t
ORIGIN      
        1 gagctcgccc agggccatgc cccgtaccgg atgaacggct tcgtcaccgt gtcggccgcg
       61 acgggcagcc agctcgagac cgattgccag agcgccgagc aggccgcggc ccgggcccat
      121 ctggagctgc ggcggctcta cggccggcag gccgaggcgc tgacctgggt gctgccgctt
      181 gcccggggga tgggatagcg cgtgcgcccg cctccggctc attcggtgac gacggcgcag
      241 ctgggggccg cgttcccgtt catcggcggg ataccggggg gctccggccc gctgctgggc
      301 gtcgaccgtt ggtcgcagcc gtttcgcttc gatgtcttcg cgctgtaccg gcacggcgcg
      361 ctgaccaacc ccaacgtcgc cgttttcggc cagctcggta ggggcaagag cagcatggtc
      421 aaatcgtggg tgcggcgcct ggcggcggcc gggtgccggg tctgggtcct cgaccccaaa
      481 ggcgagtacc gccggctggc ccgcgcccta ggcgccacgc acatcgagct tcgccccggc
      541 ggcgggatgc gcctgaatcc cctccagcat cccgtccatg ggctcgacca ggccgcctcg
      601 ctctgcgcgg cgctggccga gaccgggctg ggccggccgc tgcgtcccgt ggagcgagcc
      661 ggtctcgacg gcgccctggc caccgctcac ccgtccagcc tggtcggggt cgtgggcgct
      721 ctgctggaag gcggcgccga ggtccgcccg cccggcatgg gcccggaggc gtgggcggcc
      781 gagggccggg agatggtgtt ggagttgcgg cgcatggtgg cgggggacct ggccggcatg
      841 ttcgacgggc cgaccaacca gggcctggac cccggccggc gagccgtggt ggtggacctg
      901 tcggctgtgt acgacacgcc ggcgctcgcc ccggtcatgg tctgtgccat ggcctggatg
      961 cggtcccagc tcgcgggcgg cgcgggcggc gagaccccgc ccacgtacct cgtcctggac
     1021 gaggcgtggg ccgtcttggc gcagctgggg gcggcccgct ggttgcggag cagctggaag
     1081 ctggcccggt cgatggggct ggcgaacgtg gccgtgttgc accgcctgtc cgacctgtcg
     1141 gcgtcggggc cggccggctc ggagcagacc cggctggccg aggggctgct ggccgacacc
     1201 gagacgcggg tgctcttcgg ccagtcgccc gacgaagctc gcgtggcggg cgaacggctg
     1261 gggctcagcc cgaccgaggt cgccgtgctg ccccgcctgc cgcgcggcat ggccttgtgg
     1321 agcgtggcgg gccgttccgc tgtcatccgc cacgtgctgg acgccgagga cttggcgatc
     1381 gtcgacaccg accaccggat ggcgcccggt gccgcgccct gatcgcatgg ccgcgctggt
     1441 ggcgccgacc gccgcggtct cggtcgtcgt cgatgcctgg ttgacgggga aagggtgcgc
     1501 ctggctggcg actggtcggt ggccgggact cagcctgggc ggcggcctgg cgttcgcctt
     1561 ccggctcgtc ggccggccgg gcgacccgtc cggcgattgg cgggccgtca ccggtgggcg
     1621 atggggcccg agcgccgggc cgttctacgc gctgctcgtg gcacttcccg ctgcgggcgc
     1681 agccctgctc actggtgcca tctcggccgt gagccgccgc cgccccgtac ctcggctcgg
     1741 cccgcctcgt ccggcccggc gtggcagcct ggcccgtcgc cggcgctggg ccccggaccg
     1801 ggccggccgg tgggcgaccg tcagggacct gcggcccctg gtggtgcgcc gggcagctcc
     1861 cggtcgggtg gtcctgggcc ggcacggccg ggggctggtg gcgggtgagg ccggccattc
     1921 ggtgctggtg gtcgggccca cccagaccta caagacgtcc ggtttggtcg tgcccgccct
     1981 cctggagtgg gaggggcccg tgctcgccgc cagcgtcaag tccgacctgg cccgggtgac
     2041 cgcggggtgg cgggagcggc gggggcgggc ctgggtgttc gatccgacgg cgaccacagg
     2101 tctgcccgcg gcccgttggt cgcccctcgg cgaggcggcg aactgggccg gtgcccagcg
     2161 catggcggcg tggctgtgcg agagcggccg gtcggcgatg gaggacggcg acttctggta
     2221 cgccacggcc cagaagctgc tcgctcctct tctcctggcc gcctcggtcg gtgcccgcac
     2281 catggccgac gtcgttcgct gggtcgacga gcaggaggtg gcggaggtgg ccggcatcct
     2341 cgacgaggcg ggcgaggagg atgccctccg cgccgccgag gccacatggc ggcgggagcc
     2401 caggcagcgg ggctcggtgt tcaccacggc cgagaccgtg ctcgccgcgt ttgccgaccc
     2461 cgccgtggcc cgggcgacgg agagctgcga catcgcgccg gacgccgtgc tcgacggggg
     2521 ttggcactcg ctctacctgt gcgcgccgag ccatgagcag gaccggatgc ggccggtgtt
     2581 cacggcgctg gtccagcaga tcgtgcggcg ggcctacgcc ctgtcggcca gcggcgctgc
     2641 gcccctcgat ccccctctgc tcgtcgtcat cgacgaagcg gcccacgtcg cgcccctcgc
     2701 cgacctggac cgcaccgccg ccactgctgc tgcccacggc atccagctgg tcaccgtgtg
     2761 gcaggacctg gcccaggtgg cggcccgcta cggggcccgg gcggccaccg tggtcaacaa
     2821 ccaccgcgcc cgcctggtgc tgtccggcgt ggccgacgcg cccaccctcg agcagatcag
     2881 ccgccttgcc ggcgaagccg aggtcccggc ctcgtcggtg acgaccgccc gcgacggggg
     2941 ccggtccacc accgcctcgc acgagcgacg cccgctgctg gctcccggcg cgctgcgccg
     3001 gctgccgccg ggtgacgcgg tgttgctcta cggtcacctg cctccggccc gggtgcggtt
     3061 gcggccctgg ttccaggacc ccgccctggc ccggcggggg caggccgctc gctgatgccg
     3121 accgggcccg ggcccgcagc cgcgccctgc cgggcggcgc attgctggcc cctcgcaccg
     3181 gggagcatat ggttgaccat ccccaccggg ggcgtaggtc agtggaagac cggcagactt
     3241 ttaatctgcg cgtcgggagt tcgatcctcc ccgcccctac caagaacgtc caggtcagga
     3301 ggtagtttcg ggattacgtt cgttcagcag atagcaaatc cccaacaaat ccccaacatc
     3361 tgaactacct ttgcgggatg gccagcatcc ggaccacgcc ggagaggacg ttcgaagttc
     3421 tgtaccgcga ccctgcgggt cggcaacgct cgaagacctt caaacgcaag accgacgcca
     3481 cccagttctc tagaacggtg gaggccgaca agttccgcgg cgagtggatg gacccacgac
     3541 tggccaaggc tccgttcgcc gactacgccg ccgtctggtg ggagtcggtg caactcgccc
     3601 ccaagacccg ccagctctac aagagccaac tgaggaattg ggtgctgccg cgctttggca
     3661 ccgtgcccgt cgccgcgatc gatgcgccga tccttcgggg ctgggccaag caaatcaccg
     3721 acgcggggcg ctcggcgagc caccgggccg gttgcatcaa cgtcgtccgg ctcgtgctgg
     3781 cgacggcggt cgagaaggga gcgataaagg tcaaccccgc ctaccgcctg cgactgccga
     3841 cgccatcgcc ggcgggtcag atgcatttcc tctcgccatc tgagatcgaa gcactggcgc
     3901 atgcgatgga tgatcccttc gatgctctgc tcgtgcgatt cgcggcctat acgggcctca
     3961 gggccggcga gatctacggc ctgcgcgtgc ggtcgctgtc caaaggccgg gtcgccgtgc
     4021 tcgacagtct gtccgagctt caaggtggcg agctcgtgct ccgcggaacg acgaagaccg
     4081 ggcgcaaccg cgtcgtgccg atgcctgcgg ccctcgccga ggagatggcg gctcttgtcg
     4141 ccggacgcgc tccgaaggac ctcgtgttcg ccggacccga tggccggccc cgatcccact
     4201 ccagctacta caagaaccgg ttcaagccgg ccctggcgaa ggcaggcctc ccaaacgagg
     4261 ttcgcttcca cgatctccgg catacctgcg cggcgatgct catcggtctc ggcgcgcacc
     4321 cgaaggccat catggagcgt ctcggtcacg cctcgatcac ggtcacgctc aacacctacg
     4381 gccatctgtt ccccgagctg gaggaggccc tcaccgatgg gttgcagggg ctttgggact
     4441 cagccctccc g
//
LOCUS       DASWGG010000249         3408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_875360, whole genome shotgun sequence.
ACCESSION   DASWGG010000249 DASWGG010000000
VERSION     DASWGG010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875360"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VF954_04515"
     CDS             <1..859
                     /locus_tag="VF954_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9530378.1"
                     /translation="YAPIAVNGHPVSALALRPERSAVDPSLISGRSPEQPDEITLGAK
                     TLHAIHAKVGDTVAVRASDTTRQLRVVGKAVYPANGPSDIGGVGEGAGLTLEGLRTLV
                     PDAPENVFPIVLARGVSRASALAHFNDELGQGQIGVQAPSPSTDVENYRSVRALPLAL
                     AGLLVAMALGALIHTLVSSVRRRRRDLAVLKTLGFVRRQVGATVAWQATVLALIALAA
                     GVPLGLATGRWAWTLYAIQQGVIPEVHLPVLIVAAAFPLLIALANVAAALPARSAAAT
                     PPAALLRSE"
     gene            991..2634
                     /locus_tag="VF954_04520"
     CDS             991..2634
                     /locus_tag="VF954_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530379.1"
                     /translation="MSLLDWEGIEVAVRRPRGGAALGRIARGLASTTDRWSRPATGAC
                     AVTLALAVTGATVRLRPIGTASAAFPAAASPGGSGMGGQPDPIEGRLAVARSSNGGAT
                     VVNSAAPALPGLVPVAAYSPAAAQAGEAGAEAAALATVEEGGGIPLRAGDLAPTLTQP
                     LLSRPGTCPFDRLRVLWSAPQGSYDVGGYLAPLGPAPTAAATAVNGVVVCDGSTFEYI
                     GFEAFWDGQHWSAAGVPSAADDHGPRLPSDDTVAPPTPAQPAVTPTAGAGPASVVTGS
                     WGPAIEDLAPYVPQSLCDPTPKPGAVGLRTLLLHSFPGSRDLGIGQACDTPDGVSEHK
                     EGRAFDWGVRADVPAEDAMADRFLAWLFAPDQYGNQYAMIRRLGIMYMIWDDHIWGSY
                     AADQGWRPYGGPNPHTDHIHISFSWAGALAQTSFWTGHVADVALVAPPPGAIPPPAPA
                     PIAALAAGSAPVPPPAASAAPAPGPAPAPGSSGAPSGTGSAPPGSAAGPGGGTAGSST
                     TTSTTTTTTTAPPAAPPPAPPPSGGPVPPPPVTVTIPIP"
     gene            2657..>3408
                     /locus_tag="VF954_04525"
     CDS             2657..>3408
                     /locus_tag="VF954_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX9530380.1"
                     /translation="MYVRGGWFPTGEEAAIFRHRLGLDPEELARAAIDPGTLRRAWGF
                     ARRYRARMLLYLATIIAAALIGIVPPLIFKELIDRAIPNRDVGLVDLLFAAAVGLALF
                     QTGLGLVNRWYASAIGEGLIFDLRVELFEHVQRQPVAFFTRTQTGALLSRLSNDVVGA
                     QQTVSTLASVISDVFTLAFTLAAMVAFSWQVTLLSLVVVPGIVLLDRRLGRRLAALSR
                     QRMTLNADMTTTMTERFNVAGAMLVKLFGRPRA"
BASE COUNT          425 a         1327 c         1177 g          479 t
ORIGIN      
        1 atatgccccg atcgcggtga atggccaccc ggtctcagcg ctggccctac gaccggagcg
       61 cagcgcggtc gatccgtcgc tcatctcggg gcggtcgccg gagcaacccg acgagatcac
      121 cctgggggcc aagaccctgc acgccatcca tgccaaggtc ggtgacaccg tggccgtgag
      181 ggcatccgac acgacccggc agctccgggt ggtgggcaag gccgtctacc cggccaacgg
      241 gccatccgac atcggcgggg tcggcgaggg cgccgggctg acgctggagg ggctcagaac
      301 actggtcccc gatgcccccg agaacgtctt tccgatcgtg ctggcccgcg gggtgagccg
      361 ggcctcggcc ttggcccatt tcaatgacga gctcgggcag ggccagatcg gcgtccaggc
      421 tccgagcccg tcgaccgacg tcgagaacta tcgctcggtt cgggcgctgc ccctcgccct
      481 cgccgggctc ctcgtcgcca tggcgctcgg cgccttgatc cacaccctgg tgagctcggt
      541 ccggcgccgg cgccgagacc tggccgtcct gaagaccctg ggcttcgtgc gccgtcaggt
      601 gggcgcgacc gtggcctggc aggcaaccgt gttggccctg atcgcgctcg ccgcaggcgt
      661 gcccctcggg ctggcgacgg gtcggtgggc ctggacgctc tacgccatcc agcagggcgt
      721 cattcccgag gtgcacctgc ccgtgttgat cgtggcggcc gccttcccgc tcttgatcgc
      781 tctggccaac gtggccgccg ctctcccggc ccgttcggcg gccgccactc cgccggccgc
      841 cttgctgcga tccgagtagc gcccgtcaga gcccggccgc ggggccgcca tccgttctag
      901 tcaaaacggg ctatttccca aatagctcca ctccgtggga ggaaaacgca ccctcggtgg
      961 ggaagaccta gccaccgccg ctgaacttgc atgagcttgc tggactggga gggaatcgag
     1021 gtggcagtgc gcagaccccg ggggggcgcg gccctcggcc ggatcgcccg gggcctcgca
     1081 tcgaccactg accgctggag ccgccccgcg acaggggcct gcgccgtcac gctggcgctg
     1141 gccgtgaccg gggccaccgt gcggctgcgg cccatcggga ccgcgtcggc ggccttccca
     1201 gcggcggcct cgcccggcgg gtcggggatg ggcggccaac ccgacccgat cgaaggccgg
     1261 ctggccgtgg cccgctcgag caacggcggc gccacggtcg tcaacagcgc cgcgcccgcc
     1321 ctgcccgggc tcgtcccggt tgctgcctac tcccccgcag ccgcccaggc gggcgaggcc
     1381 ggcgcagagg ccgccgccct ggccaccgtc gaggaagggg gcgggatccc gctgcgggcc
     1441 ggcgacctgg cccccaccct cacccagccg ctgctgtcca ggccgggtac ctgcccgttc
     1501 gaccggctgc gggtgctgtg gtcggcccca cagggcagct atgacgtcgg cggctacctc
     1561 gcgcccctcg gtccggcgcc cacggcggcg gcgaccgccg tgaacggcgt ggtggtctgc
     1621 gacgggtcga cgttcgagta catcggattc gaggcgttct gggacggcca gcactggtcg
     1681 gcggcgggcg tcccctcggc cgccgacgac cacggaccgc gccttccgag cgacgacacc
     1741 gtggccccgc cgaccccggc ccaacctgcg gtcaccccga ccgccggtgc ggggccggcc
     1801 tcggtcgtga ccggttcctg ggggcccgcc atcgaggacc tggcgcccta cgtgccgcag
     1861 tccctgtgtg accccacccc gaagccaggc gcagtgggcc tgcggacgct gctcctgcac
     1921 agcttccccg ggtcccggga cctcggcatc ggccaggcgt gcgacacccc cgacggggtc
     1981 tccgagcaca aggagggccg ggccttcgac tggggggtgc gggcagacgt gcccgccgag
     2041 gacgccatgg cggaccgctt cctggcctgg ctcttcgccc cggaccagta cggcaaccag
     2101 tacgccatga tccgccggct cggcatcatg tacatgatct gggacgacca catctggggc
     2161 tcctacgcag ccgaccaggg ctggcgcccg tacggcgggc ccaacccgca caccgaccac
     2221 atccacatca gcttcagctg ggccggcgcc ctggcccaga cgagcttctg gaccggccac
     2281 gtggccgacg tagcgctggt ggcgcccccg cccggggcca ttcccccgcc ggcgccggcg
     2341 cccatcgcgg cgctggcggc gggctcggca cctgtgcccc cgccggccgc ttcggccgct
     2401 cccgctcccg gcccggctcc cgctcctggc tcttcgggag ctccgagcgg gacggggtcg
     2461 gcaccacccg ggagcgcggc cgggccgggc ggcggcaccg ccgggtcgag cacgacgaca
     2521 tctacgacca ccaccaccac gaccgcacca ccggcggcac ccccgcctgc gcccccgccc
     2581 tccggcggac cggtgccgcc cccacccgtc actgtgacca tcccgatccc gtagcgcccc
     2641 gggcgcacat ggcggaatgt acgtccgtgg cggctggttc cccacgggcg aggaggccgc
     2701 cattttccgc catcgactgg ggctcgaccc cgaggagctc gcccgggccg ccatcgaccc
     2761 cggaaccctg cgacgggcgt ggggcttcgc gcggcgctac cgggcccgga tgctgctgta
     2821 cctggcgacg atcatcgccg ccgccctgat cggcatcgtg ccgccactga tcttcaagga
     2881 gctgatcgac cgggccatcc cgaaccgcga cgtcgggctg gtggacctgc tctttgccgc
     2941 ggccgtgggc ctggccctgt tccagaccgg gctcgggctg gtcaaccgct ggtacgcgtc
     3001 ggccatcgga gaggggctca tcttcgacct ccgggtagag ctgttcgagc acgtccagcg
     3061 ccagcccgtg gcgttcttca cccgcacgca gaccggggcg ctgctgtccc ggctgtccaa
     3121 cgacgtcgtc ggcgcccagc agacggtgtc caccctggcg agcgtcatct ccgacgtctt
     3181 cacgctggcc ttcaccctgg ccgccatggt cgcgttcagc tggcaggtga ccctgctgtc
     3241 gctcgtggtc gtgcccggca tcgtcctgct cgatcgccgc ctgggccggc gcctggccgc
     3301 cctgtcgcgg cagcgcatga ccctcaacgc ggacatgacc acgaccatga ccgagaggtt
     3361 caacgtggcc ggcgccatgc tggtcaagct gttcggccgg ccccgggc
//
LOCUS       DASWGG010000250         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3132273, whole genome shotgun sequence.
ACCESSION   DASWGG010000250 DASWGG010000000
VERSION     DASWGG010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3132273"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..881)
                     /locus_tag="VF954_04530"
     CDS             complement(81..881)
                     /locus_tag="VF954_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9530381.1"
                     /translation="MAYRHQRVQRLRRLLQRPATRQAEGAFVIEGAKLLAEALDAGVR
                     VESVYLEPVAEPGSSGLAALADRALADGARVFTLAGDVMARVADTTTPQPVMAVVETV
                     TAGLEELKGAKLVLVLADVRDPGNAGTVVRCAEAAGAEGVVFCRGSVDVYNPKTVRAS
                     AGSLFHVRVVVGGGPLDVLDRMGGWGMQRLAAVARTGRAHTEADLTRPTAFLLGNEAG
                     GLDPASVAACDDTISIPMVGRAESLNVGMAAAVLCFEATRQRAQVPHG"
     gene            complement(893..1375)
                     /gene="rplT"
                     /locus_tag="VF954_04535"
     CDS             complement(893..1375)
                     /gene="rplT"
                     /locus_tag="VF954_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX9530382.1"
                     /translation="MARVKRAVHGKKHRRAVLEAASGYYGNKSRSFRAANEQVMHSLQ
                     YAYRDRRARKGDFRQLWIQRINAASRQHGMSYSRFIAGLREAEIEVDRKVLADLAVTD
                     PAAFATLVRLARGERVPEAAMAPSSASSADPASTSTSTSGEAAPAEAADEASPEEQTA
                     "
     gene            complement(1433..1627)
                     /gene="rpmI"
                     /locus_tag="VF954_04540"
     CDS             complement(1433..1627)
                     /gene="rpmI"
                     /locus_tag="VF954_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX9530383.1"
                     /translation="MPKMKTDKGAAKRIKITGTGKLRRRHAFMNHILEKKPSTRTRRL
                     NRESDVAPGDARQVKRLLGL"
BASE COUNT          200 a          582 c          598 g          247 t
ORIGIN      
        1 ccgtcgcggc cgggttggcc acgacgatcg tgcggtcccg atcgagcacc agcacggcgt
       61 cggggagcag ctccgtgtag tcagccatgg ggcacctgcg cccgctggcg ggtcgcctcg
      121 aagcacagca ccgccgcggc cattccgacg ttgagcgact cggcccggcc caccatcggg
      181 atggagatgg tgtcgtcgca cgccgccacc gacgccggat cgaggccacc ggcctcgttg
      241 cccaggagga aggcggtcgg tcgggtcagg tcggcctcgg tgtgggcgcg gcccgtgcgg
      301 gccaccgccg ccagccgctg catcccccac cctcccatcc gatcgagcac gtccagtggc
      361 ccgcctccga ccacgaccct cacatggaac agcgagccgg ccgacgcgcg cacggtcttg
      421 gggttgtaca cgtccaccga cccgcggcag aacactaccc cctcggcgcc ggcggcctcg
      481 gcgcagcgga cgaccgtccc cgcattgccc ggatcgcgca catcggccag cacgagcacc
      541 agtttggccc ctttcagctc ctccaggccg gccgtgaccg tttcgaccac cgccatgacc
      601 ggttgggggg tggtggtgtc ggccacccgg gccatcacgt cgccggccag ggtgaacacc
      661 cgggccccgt cggcgagcgc ccggtccgcc agcgcggcca gcccgctcga tcccggctcc
      721 gccaccggct cgaggtagac cgactcgacc cgcacgccgg catcgagagc ctcggcgagc
      781 agcttggctc cctcgatgac gaacgccccc tcggcctgac gggtggcggg gcgctgcaag
      841 aggcggcgca gccgctgcac ccgctggtgg cggtaggcca acgcgggctg gttcaggccg
      901 tctgctcctc ggggctggcc tcgtcggcgg cttcggccgg tgccgcttcg ccggaggtgg
      961 aggtggatgt ggaggcgggg tcggctgagg aggccgagga gggggccatg gccgcctcgg
     1021 gcacccgctc gccccgcgcc aggcggacca gggtggcgaa ggccgccggg tcggtgacgg
     1081 ccagatcggc gaggaccttg cgatcgacct cgatctcggc ttcccgcagc ccggcgatga
     1141 agcggctgta gctcatcccg tgctggcggc tggcggcgtt gatgcgctgg atccagagct
     1201 ggcggaagtc gcccttgcgc gccctgcggt cgcggtaggc gtactgcagg gagtgcatga
     1261 cctgctcgtt ggcggcccgg aagctgcggc tcttgttgcc gtagtatccc gacgctgctt
     1321 cgagcaccgc ccggcggtgc ttcttgccgt gcacggcgcg cttgaccctg gccatcggct
     1381 ggctcctttc gtgagggcgg gacctgcgtc ccgcaaggtg gggggaaggg gctcagagcc
     1441 cgagcaggcg cttgacctgc cgggcgtcgc cgggcgccac gtcggactcg cggttgagcc
     1501 ggcgggtgcg cgtcgagggc ttcttctcga ggatgtggtt catgaaggcg tggcggcgcc
     1561 ggagcttgcc cgtcccggtg atcttgatgc gcttggcggc gcccttgtcg gtcttcatct
     1621 tcggcat
//
LOCUS       DASWGG010000251         5257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1519984, whole genome shotgun sequence.
ACCESSION   DASWGG010000251 DASWGG010000000
VERSION     DASWGG010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519984"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VF954_04545"
     CDS             complement(<1..766)
                     /locus_tag="VF954_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9530384.1"
                     /translation="MSITEDSHQVVIIGAGPAGLTAAYCLARRGQTATVLEADRVVGG
                     ISRTVERDGWRFDIGGHRFFTKVGPVEELWHEILPDEEFLMRPRMSRIYYGGKFFDYP
                     LKASNALRNLGVVEACKCVASYAWAQVRPPKDQTNYEGWLVARFGWRLYRTFFKTYTE
                     KVWGIPVAQMPADWAAQRVKSLSLGKAITNSLLPRRNQKEITSLIEEFQYPKLGPGMM
                     WERAHELVRRAGTEVVFDTKVTAIRHRGGRAHAVATT"
     gene            complement(805..2004)
                     /locus_tag="VF954_04550"
     CDS             complement(805..2004)
                     /locus_tag="VF954_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530385.1"
                     /translation="MSTSIATPVPEPEGAPEPQAAPTRPRPAGASLWADLRAALPGWV
                     VARVVVGVALVGTRWYLRLTREGDPTVLTRSRQGLMSWDAAWYRGIALHGYAGVGREG
                     LRFFPLWPLAGRALAAPFGGGGAVTVGLLLAANVLSLGLGALVHRLVVAETGNVPLAR
                     RAAWLTAIAPPASVLVMGYSEPMAISLAVLAFLALRSRRWWWAASAGLLYGLSRPVGV
                     LLVVPAAIEAARHLRRVPAGEKVARAAAVLAAPAGAALYLAWVGGRFGDAMLPYRVQG
                     AGHLRGALADPAEVLVHAGARLLRGDLGSQGHYTWALGLVALAVVCGRRWPGSYTAFV
                     AATLLAVLASHNLNSLERYLLGAFPIVLTGAGLVRTRRGEVAAAAASLALLGVYAAAA
                     FSSQYVP"
     gene            complement(1973..3562)
                     /locus_tag="VF954_04555"
     CDS             complement(1973..3562)
                     /locus_tag="VF954_04555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9530386.1"
                     /translation="MTEVRDRTAALPALDVPDVGPGLRGRDWTRWVVAAAALAVAVGV
                     VLRFFATSDLWLDESLSVNISRLPIARIPAALRQDGAPPAYYVLLHLWMQLFGDGNVA
                     VRALSGIFSVAALPLAWRAGLRLGGRVAAGATLVLLATAPYSTRYATEGRMYSLVSLL
                     VLGGFLLVSRCLTRPTPGRLLALSLLTGTLLLTHYWGFYLLLVVGGWLLWQGFGGTRV
                     AGATDARNARMVLLALGGGGLLFLPWLPTFLFQLAHTGTPWGGGAALNAMVDTVSEYA
                     GGNATGGPFLFLLLVGLIALALFARPIDDHRVELDFRTRPRARPLAIAGFATLALAVT
                     VGSVTNSAYEVRYTAVAFPLVILLAGLGATALVDRRVRNLFLAAAALLGLTTGMSVAT
                     TDRTQAGQVGRIISAQARADDVVAYCPDQLGPAVSRVVKAPVTQLTYPRWLPPDRIDW
                     VDYAQVNRSADAASFALRLVQAAGPHDIWLVWQNGYKTFRGRCAALAKDLALLRPGDV
                     PEVIARTSVLEHEYLDRYPSP"
     gene            complement(3722..4945)
                     /locus_tag="VF954_04560"
     CDS             complement(3722..4945)
                     /locus_tag="VF954_04560"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9530387.1"
                     /translation="MSWSTYRMQSTFADLGVSPGLAGRLESRGILAPFPIQEAAIPPA
                     LAGRDIAGRAPTGSGKTLAFGLPIAQRVERARPGRPRALILAPTRELAAQIRDELAPL
                     LATRQRSVAAFYGGTSYVPQRKLLNRGVDVLVACPGRLADLMARGNVTLDEVQTVVVD
                     EADRMADMGFLPDVRRILDKVPGQRQTLLFSATLDGAVDVLIRRYQRDVLRCDVAAPD
                     PADDATVHRFVMVAQQQRIGLTADLIGEHGSTLVFCRTKRGADRVAAQLAKAGVGAVA
                     IHGDRSQSQRERALAAFSGRRVQALVATDVAARGIHVSDVGCVVHFDPPADVKDYVHR
                     SGRTGRAGASGVVVSLVTDADKLKVRQLQRALGLAREEGPAVRPDREAHRPAQVASRR
                     ARGTAGSRRRAQRPA"
BASE COUNT          797 a         1889 c         1967 g          604 t
ORIGIN      
        1 ccgtcgtggc caccgcgtgg gccctgccgc cccggtgacg gatggcggtc accttggtgt
       61 cgaagacgac ctcggtccct gcacgccgca ccagctcgtg ggcccgctcc cacatcatcc
      121 cgggcccgag cttggggtac tggaactcct cgatgaggct ggtgatctcc ttctggttgc
      181 gcctcgggag cagggagttg gtgatcgcct tgccgagcga gaggctcttg acccgctggg
      241 cggcccagtc ggcgggcatc tgggcgacag ggatgcccca caccttctcg gtgtaggtct
      301 tgaagaaggt gcggtagagc cgccacccga agcgggccac gagccagccc tcgtagttgg
      361 tctggtcctt cggcgggcgg acctgcgccc acgcgtacga cgccacgcac ttgcacgcct
      421 cgaccacgcc gaggttgcgc agggcgttgg aggccttgag cgggtagtcg aagaacttgc
      481 cgccgtagta gatccggctc atccggggcc gcatgaggaa ctcctcgtcg ggcaggatct
      541 cgtgccacag ctcctcgacc gggcccacct tggtgaagaa acggtgcccg ccgatgtcga
      601 agcgccaccc gtcccgttcc acggtgcggc tgatgccccc gaccacccgg tcggcctcga
      661 ggacggtggc cgtctgtccc cgccgggcca ggcagtaggc cgcggtcagc ccggccggtc
      721 ccgccccgat gatcacgacc tggtggctgt cctcggtgat gctcacagcg ggccatgctc
      781 gctgctccgg cccgtggccg ccagtcacgg cacgtactgc gacgagaagg ccgccgcggc
      841 gtacacgccg agcagcgcga gcgacgcggc cgccgcggcc acctcgcccc ggcgggtccg
      901 gaccagcccg gcgccggtca gcacgatggg aaaggcgccg agcaggtagc gctcgagcga
      961 gttcaggttg tgggacgcca gcacggcgag cagggtggcc gccacgaacg ccgtgtagga
     1021 cccgggccat cgccggccgc acaccacggc cagcgccacc agcccgaggg cccacgtgta
     1081 gtggccctgg gagccgaggt cgccgcgcag cagccgcgcg cccgcgtgga ccaggacctc
     1141 ggccgggtcc gccagggcgc cgcgcaggtg cccggccccc tggacccggt acggaagcat
     1201 cgcgtccccg aagcgcccac cgacccaggc caggtacagc gcggcgccgg ccggtgcggc
     1261 caggaccgct gccgcccggg ccaccttttc ccccgcggga acgcggcgca ggtgacgggc
     1321 ggcctcgatg gccgcgggaa cgaccagcag gacgccgacg gggcggctca gcccgtacag
     1381 caggccggcc gaggcggccc accaccaccg gcggctgcgc agggcgagga aggcgagcac
     1441 ggccagggag atggccatcg gctcggagta ccccatgacc agcacggacg ctggcggcgc
     1501 gatcgccgtg agccaggcag ccctcctggc cagggggacg ttcccggtct ccgccacgac
     1561 gagccggtgg accagggcgc cgaggcccag ggacagcacg ttggcggcca gcagcagccc
     1621 gacggtcacg gcccccccgc ctccgaacgg tgcggccagg gcgcgcccgg cgagcggcca
     1681 caacgggaag aagcgaagac cctctctgcc cacccccgcg tacccgtgca gggcgatgcc
     1741 gcggtaccac gccgcgtccc agctcatcag gccctgccgg gaccgggtga gcaccgtggg
     1801 atcgccctcg cgcgtcagcc gcaggtacca ccgggtgccg accagcgcca ctcccaccac
     1861 gacccgggcg acgacccagc ccgggagggc ggcgcgcagg tcggcccaga ggctggcgcc
     1921 ggccggccgt gggcgcgtgg gagcggcctg gggctcaggg gcgccttcgg gctcagggac
     1981 tggggtagcg atcgaggtac tcatgctcga ggaccgacgt ccgggcgatg acctcgggta
     2041 cgtcgcccgg gcgcagcagg gccaggtcct tggccagggc ggcgcagcgg ccccggaacg
     2101 tcttgtagcc gttctgccag acgagccaga tgtcgtgggg gccggccgcc tgcaccaggc
     2161 gcaacgcgaa cgaggcggcg tcggccgacc ggttgacctg ggcgtagtcg acccagtcga
     2221 tgcgatcggg ggggagccac cgcggatagg tgagctgggt cacgggcgcc ttcaccaccc
     2281 ggctgacggc gggcccgagc tggtccgggc agtaggccac cacgtcgtcg gcccgcgcct
     2341 gggccgagat gatgcggccg acctgcccgg cctgggtgcg gtcggtggtc gccacgctca
     2401 tgccggtcgt gaggccgagg agcgcagcgg ccgcgaggaa caggttgcgg acccggcggt
     2461 ctaccagggc ggtggccccc agaccggcga gcaggatgac gagagggaac gccaccgccg
     2521 tgtaccgcac ctcgtaggcc gagttggtga ccgagccgac ggtgacggcc agggcgaggg
     2581 tggcgaagcc ggcgatggcg agagggcggg cccgcggccg ggtccggaag tcgagctcca
     2641 cccgatggtc gtcgatgggc cgggcgaaca gggccagggc gatcaggccc acgagcagca
     2701 ggaagaggaa cggaccgccg gtcgcgttcc cccccgcgta ctcgctgacg gtgtcgacca
     2761 tggcgttgag ggccgctccc ccgccccagg gcgtgcccgt gtgggccagc tggaacagga
     2821 aggtcggcag ccagggaagg aacagaagcc caccgccacc cagggccagc agcaccatcc
     2881 gggcattgcg ggcatcggtg gcgccggcca cacgggtacc gccgaaaccc tgccacagca
     2941 gccacccgcc gaccaccagg agaaggtaga aaccccagta gtgggtcagc agcagcgtcc
     3001 ccgtgagcag gctgagggcg agcagccggc ccggcgtggg gcgggtcagg caccggctga
     3061 ccagcaggaa gccgccgagg accagcagcg acacgagcga gtacatccgg ccctcggtcg
     3121 cgtaccgggt gctgtagggc gcggtggcca gcaggaccag ggtggcaccg gctgcgaccc
     3181 ggcccccgag gcgcaatccc gcccgccagg caaggggcag ggcggccacg gagaagatgc
     3241 ccgacagggc gcgcaccgcg acgttgccgt caccgaacag ctgcatccac aggtggagca
     3301 gcacgtagta cgccggcggg gccccgtcct ggcggagcgc ggcggggatg cgggcgatcg
     3361 gcaggcgcga gatgttgacc gagagcgact cgtccagcca caggtccgag gtggcgaaga
     3421 agcgcagcac caccccgacg gccacggcca gggcggcagc ggccacgacc cagcgggtcc
     3481 agtcccggcc gcggagaccg ggccccacat caggcacgtc gagcgcgggg agggctgcgg
     3541 tccggtcccg gacctcggtc atctccacgg ccgtgaggct aattcggcgc gggcgcgagc
     3601 gacattcatg cccggctccg cagtggaggc tcgcggccgc tcgtgaaacc attctgttga
     3661 cgcggggtgc ccttataggg ctctacgcgt caacagaatg gccaggacca gaactggcgg
     3721 gtcaggcggg ccgctgcgcc cggcgcctcg accccgccgt ccctcgggcg cggcgcgacg
     3781 ccacctgggc cggccggtga gcttcgcggt ccggccgtac cgccggtccc tcctcgcggg
     3841 ccaggccgag cgcccgttgc agctggcgca ccttgagctt gtccgcgtcc gtgaccagcg
     3901 acaccaccac ccccgatgcg ccggcccggc ccgtgcgccc cgagcgatgg acgtagtcct
     3961 tcacgtcggc cggcgggtcg aaatgcacga cgcagcccac gtcggagacg tggatgccgc
     4021 gagcggccac atcggtcgcc accagcgcct gcacccggcg ccccgagaag gcggccagcg
     4081 cccgctcgcg ttgggactgg ctgcggtcgc cgtggatggc cacggcgccg acccccgcct
     4141 tggccagctg ggccgccacc cgatcggcgc cccgcttcgt gcggcagaac accagcgtgg
     4201 acccgtgctc gccgatgaga tcggccgtga gcccgatgcg ctgctgctgg gcgaccatca
     4261 cgaaccggtg gaccgtggcg tcgtcggccg ggtcgggggc tgccacgtcg cagcgaagta
     4321 cgtcgcgctg gtagcgccgg atgagcacgt cgacggcgcc atcgagggtg gccgagaaca
     4381 gcagcgtctg gcgctggccc ggcaccttgt cgaggatacg gcgcacgtcc ggcaggaagc
     4441 ccatgtcggc catgcggtcg gcctcgtcca ccaccacggt ctggacctcg tcgagggtca
     4501 cgttgcccct cgccatgagg tcggcgaggc ggcccgggca agccaccaag acgtccacgc
     4561 cgcggttcaa cagctttcgt tggggcacgt agctggtgcc gccgtagaac gcggccacgc
     4621 tgcgctggcg ggtggccagc agcggcgcca gctcgtctcg tatctgggcg gcgagctcgc
     4681 gcgtcggagc caggatcagg gctcgggggc ggcccggacg cgcccgctcg acgcgctggg
     4741 cgatgggcag cccgaaggcc agcgtcttgc ccgagccggt gggcgcgcga ccagcgatgt
     4801 cccggccggc gagggcgggc gggatggccg cctcctgaat ggggaacggg gccaggatcc
     4861 cccgggactc gaggcggccg gcaaggccgg gagagacgcc caggtcggca aaggtcgact
     4921 gcatgcggta ggtgctccaa ctcatcgtgc gccgcacgga ggcggcgggc cgcgacaggc
     4981 ccagcggtga cctcggcaca cgatgccgcc ccgtcggagc gcatcgcggc tgcgtcgcgg
     5041 cagcagaggc gtgaccgcag gacaggagcc ccgaacggcc cgtcggcttg caccgacgcc
     5101 agcgggccgt gagcccactg cttgtctctc atggtaccag cccgatcagc tggcgggcag
     5161 gggccgggcg aagctcacct catggccctg cggatcgcgc atgtggaagt accgctcacc
     5221 ccacggagca tcggctggtg cggcctcggg caccagg
//
LOCUS       DASWGG010000252         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1204797, whole genome shotgun sequence.
ACCESSION   DASWGG010000252 DASWGG010000000
VERSION     DASWGG010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204797"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VF954_04565"
     CDS             <1..593
                     /locus_tag="VF954_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9530388.1"
                     /translation="LGPAVSAVAGDVADGAHREALVAEARRLGPVRALVNNASTLGPG
                     PLPQLATVGPGELAAVLDVDVVAPLALAQLVLPDLRANRGVVVNVTSDAAVEAYEGWG
                     VYGSAKAALEQWSAVLAVEEPDVRVYAVDPGDMRTRMHQDAFPGEDISDRPEPGASVP
                     GLVALLDGTRPSGRYRAREVEVAVPATPAVNAGAGS"
     gene            590..1762
                     /locus_tag="VF954_04570"
     CDS             590..1762
                     /locus_tag="VF954_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX9530389.1"
                     /translation="MTPAASLVGGALGAGPLDFVLPPGLEAHEPPEARGVARDGVRLL
                     VAAGGRMAHSVASALPGFLEPGDLLVVNLSSTLAAAVEGMGDDGRPLAVHFSGPHPGG
                     GWVVELRGVITAGASAAFAAADAGEVVRLPAGARLRLVEPEAMAPGMEAPRLWRADLD
                     QPGLRSRVGRRSLSTADDLARVGGWLAQHGAPIRYAHVPRPWPLSAYQTVFARVPGSA
                     EMPSAGRPFTAALLAALAARGVGVAPVVLHAGVSSQEAGEGPLTEPFAVPGPTAERVN
                     DAHRRGHRVVAVGTTVVRALESAVGSTGAVEARRGWTDLVVTPDRGVGAVDGLVTGWH
                     EPRASHLALLEAIAGRPLLEASYRAALAEGYLWHELGDLHLILPPIGGRQGAVAPR"
     gene            1795..>2072
                     /locus_tag="VF954_04575"
     CDS             1795..>2072
                     /locus_tag="VF954_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530390.1"
                     /translation="MVLDGGAVVVVVVRRGGAVVVEAGGVVVVGPGGLVVAVAKGTVV
                     SAALPPGAGPPVPAPPPEPGAAGKVVPADAVVMVGPTVPSAPEDRRRAS"
BASE COUNT          211 a          763 c          838 g          260 t
ORIGIN      
        1 agctgggccc cgccgtgtcg gccgtggcgg gggacgtcgc tgacggcgcg caccgcgagg
       61 cgctcgtggc cgaggcccgc cgcctgggac ccgtccgggc gctggtcaac aacgccagca
      121 ccctcgggcc gggcccgttg ccccagctgg ccaccgtagg cccgggggag ctggcggccg
      181 tgctcgatgt cgacgtcgtg gcgccgctcg ccctggccca gctcgtgctc cccgacctgc
      241 gcgccaaccg gggcgtcgtg gtcaacgtca catcggacgc ggcggtcgag gcctacgagg
      301 gatggggcgt ctacggctcg gccaaagccg ccctcgagca gtggagcgcg gtgctggccg
      361 tcgaggagcc ggatgtccgg gtctatgccg tggacccggg cgacatgcgg acccggatgc
      421 accaggacgc cttcccgggc gaggacatct ccgaccggcc cgagccgggc gcgagcgtgc
      481 ccggcctggt cgccctcctt gacggcacca ggccgagcgg tcggtaccgg gcccgggagg
      541 tcgaggtggc ggtccccgcg acgcccgccg tcaatgcagg agccgggtca tgacgccggc
      601 ggcctccctg gtggggggag ccctgggcgc cggcccgctc gacttcgtgc tcccgcccgg
      661 gctggaggcc cacgagccgc ccgaggcccg cggcgtcgcc cgagacggcg tgcggctcct
      721 ggtggcggcg ggcgggcgga tggcccactc ggtggcgtcg gccctgccgg ggttcctcga
      781 gcccggcgac cttctcgtgg tcaacctgtc ctcgaccctc gccgccgcgg tcgagggtat
      841 gggcgacgac ggccgccccc tggccgtcca cttctccggc ccgcaccccg gcggggggtg
      901 ggtggtcgag cttcgcggcg tgatcaccgc cggagcctcc gccgccttcg cggcggccga
      961 cgccggcgag gtcgtccggc tcccggcagg agcgcggctg cgcctcgtcg agccggaggc
     1021 gatggccccg gggatggagg cgccccgcct gtggcgggcc gacctagatc agcccggcct
     1081 ccgcagccgc gtggggcgga ggagcctgtc gacggccgac gatctggccc gggtcggggg
     1141 gtggctcgcc caacacggcg ccccgatccg ctacgcccac gtgccgcggc cgtggccgct
     1201 gtccgcgtac cagacggtgt tcgcccgggt gccgggaagc gccgagatgc cgagcgcagg
     1261 ccggcccttc accgcagccc tgctcgcggc gctcgcggcc cgcggcgtcg gagtggcacc
     1321 ggtcgtgctc cacgccggcg tctcgtccca ggaggcggga gaggggccgc tgaccgagcc
     1381 cttcgccgtg cccggcccga cggccgaacg cgtgaacgac gcccaccggc gaggccaccg
     1441 cgtggtcgcg gtcggcacga ccgtggtgcg cgccctcgag agcgccgtgg gcagcaccgg
     1501 tgccgtcgag gcccggcggg gctggaccga cctggtcgtg accccggaca ggggcgtggg
     1561 cgcggtggac gggctggtga cgggctggca cgagccgcgc gcttcacacc tggccctgct
     1621 cgaggccatc gccggacgcc cgctcctcga ggcgtcctac cgggccgccc tggccgaggg
     1681 gtacttgtgg catgagctcg gtgacctgca cctgatcctc cccccgatcg gggggaggca
     1741 gggcgcggtc gcgccgcgat aggggaggga gccgctcagg ccaaggccgt cgtggtggtg
     1801 ctggacggcg gtgctgtggt ggtggtcgtg gtccgacggg gcggggccgt ggtcgtcgag
     1861 gccggtggcg tggtcgtggt cggcccgggc gggctggtcg ttgcggtcgc gaaggggacg
     1921 gtggtgtcgg ccgcgctccc gccgggtgcc gggccgccgg tccccgcgcc cccacccgag
     1981 cccggagcgg cgggcaaggt cgtgcccgct gacgcggtcg tcatggtcgg cccgacggta
     2041 cccagcgctc cggaagaccg acggagggcg tc
//
LOCUS       DASWGG010000253         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2047271, whole genome shotgun sequence.
ACCESSION   DASWGG010000253 DASWGG010000000
VERSION     DASWGG010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047271"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="VF954_04580"
     CDS             <1..1543
                     /locus_tag="VF954_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530391.1"
                     /translation="FEGVSIDAALTPADPARPVELRVAVRGLRVEGKVLPPVVLDPAR
                     LDRDVVPLLQNLLQDRLGRLAGEPGAPQPLRALAEHLLPLVGLGGDVPRLPVEGIGID
                     PSVVARWFTTVLDSPPAGGGTPGSAWLGHLAGLLGSGAPVAGTGAEADPYRVLLATFG
                     GARVDLTLATRTAAGGRDLLAGVALLLGGPAGAVLEARGMLLSLPLTGPGPPRAVPSA
                     AVTIRAPGDAAGTLVAGGGLGVRFVRAGLAWDGIALGPILELGGVEFEGASHELINLS
                     NVDSIRAAAAEAARASIAAALGTDGPGSHLAVLVGLIPPPGEPGAPVADLVALLSAPT
                     RELARVHRSRLHDAAHSWATMLAHLAALLGLPEPVAGTGSPAEPWHVPVAKDGPLTLE
                     LAAWTGDHDLHLGLRAVATPTVGAATIDATMLSEVVHLTFPDSGPTTARLLGRHQGRL
                     AVTGPFTLAPVGGIGLTVQGGDLTIDWSGTGARAGRDTVRAHRAPRRRRRDAAGVAGA
                     AGRWL"
     gene            1479..>2286
                     /locus_tag="VF954_04585"
     CDS             1479..>2286
                     /locus_tag="VF954_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530392.1"
                     /translation="MHLDDGDVTLPALRVPPAAGFDPALPDLGLGIPVTDLDRVARAL
                     LARLWGSVAGDDGFLASALLGLRHGFGDLPADWPVPELPAGGLGALLADPLGVLRTWL
                     TRLLLDLSVNRVPFALGALDGLRRLLSPGANADLGAEGAGTRAAPWRVDLADPGSSQL
                     LLWLEPDGPPAAWATDAADQATDDLTGDGLLALMSRVGQPLAPLAALFGGPQGPSTPD
                     RLSDAFAALVSRCSDGDGVVPLAAQFPDDSAWALGETLVDAAHHRLPEHAD"
BASE COUNT          234 a          895 c          827 g          330 t
ORIGIN      
        1 cttcgagggc gtctcgatcg acgccgcgct cacaccggcc gaccccgccc ggccggtgga
       61 gttgcgtgtg gccgtacgcg gccttcgggt cgaggggaag gtgctcccgc ccgtggtcct
      121 cgatccggcg cggctggacc gtgacgtcgt accgctgctg cagaacctgc tgcaggaccg
      181 gctgggccgc ctggccggcg agccgggcgc gccccaaccg ctccgtgcgc tcgccgagca
      241 tctcctgccg ctcgtcggtc tcggcggcga cgtaccgcga ctgccggtcg aaggcatcgg
      301 catcgacccg tccgtggtcg cccggtggtt caccaccgtg ctggactcgc ccccggccgg
      361 cggcgggaca cccggatcgg cgtggctcgg ccacctcgcc ggtctgctcg gctccggcgc
      421 cccggtcgcc ggcacggggg ccgaggccga tccgtatcgg gtgctcctgg ccacgttcgg
      481 cggcgcccgg gtcgacctga ccctcgccac ccgaaccgcc gccggcggtc gcgatctgct
      541 ggccggggtc gccctgctgc tgggcggccc ggccggggcc gtcctggagg cccgcggcat
      601 gctgctctcg ttgcccttga ccgggccggg accgccccgt gcggtgccgt cggccgccgt
      661 cacgatccgg gcgcctggcg atgcggcggg cacgctggtt gccggcggcg ggctgggagt
      721 ccgctttgtt cgggccggcc tggcgtggga cgggatcgcc ctgggaccga tcctcgagct
      781 gggcggtgtc gaattcgagg gcgcttctca cgaactgatc aatctatcca atgtggacag
      841 catccgggcc gccgccgcgg aggccgcacg ggcatcgatc gcggccgccc tgggcaccga
      901 cggaccgggt tcacatctcg cggtgctcgt cgggctgatc cctccgcccg gcgagcccgg
      961 cgcgccggtg gccgacctgg tggccctgct gtcggcgccg acccgggaat tggcccgggt
     1021 tcaccgatcc cgcctgcacg acgcagccca ctcctgggcg accatgctgg cccacctcgc
     1081 tgccctgctc ggcctgcccg agcccgtagc cggcaccggc tcgccagcgg agccgtggca
     1141 cgtcccggtg gcgaaggacg gacccctcac cctggagctg gccgcctgga ccggcgacca
     1201 cgaccttcac cttggcctgc gcgccgttgc caccccgacc gtgggcgccg cgacgatcga
     1261 cgcgaccatg ctgtccgagg tcgtccacct caccttcccg gactccgggc cgaccactgc
     1321 ccgcctgctc ggccggcatc agggccgcct cgcggtgacg ggaccgttca cgctggcgcc
     1381 ggtcggtggc atcggtctca cggtccaggg cggcgacctc accatcgact ggagcgggac
     1441 aggtgctcgc gccggtcgcg acactgtccg ggctcaccgt gcacctcgac gacggcgacg
     1501 tgacgctgcc ggcgttgcgg gtgccgccgg ccgctggctt tgatccggcc ctgcccgacc
     1561 ttggcctcgg gatcccagtc accgacctcg accgggtggc gcgggcgctg ctggcccggc
     1621 tctggggatc ggtggccggc gacgacgggt tcctggcgag cgcgctgctc ggcctgcggc
     1681 acggcttcgg cgacctgccc gccgattggc ccgtacccga actccccgcg ggcggtctcg
     1741 gtgccctgct ggccgatccg ctcggcgtgc tccggacctg gctgacccgg ctcctgctcg
     1801 acctgtcggt gaacagggta ccgttcgcgc tgggtgcgtt ggacgggctg cgccggctgc
     1861 tgagccctgg cgcgaacgcg gacctcggcg ccgagggggc gggcacccgg gccgcaccat
     1921 ggcgggtcga cctcgccgat ccgggcagtt cgcagctgct gctgtggctg gagcccgacg
     1981 gaccgcccgc cgcgtgggcg accgacgcgg ccgaccaggc gaccgacgat ctcaccggcg
     2041 acgggctgct cgccctcatg agccgggtcg gccaaccact cgccccgctc gccgccctct
     2101 tcggagggcc gcagggaccg tcgacccccg accggctgtc cgacgccttc gccgcgctgg
     2161 tcagccggtg ctccgacggc gacggcgtgg tccccctcgc tgcgcagttc ccggacgact
     2221 cggcatgggc gctgggcgag acgctggtcg acgccgccca ccaccggctc cccgagcacg
     2281 ccgaca
//
LOCUS       DASWGG010000254         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_877068, whole genome shotgun sequence.
ACCESSION   DASWGG010000254 DASWGG010000000
VERSION     DASWGG010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877068"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VF954_04590"
     CDS             <1..1017
                     /locus_tag="VF954_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX9530393.1"
                     /translation="NGAAQLAVRARAGLRERFLQAHLQGPVEEQCRFTVERLGAYVGG
                     GLAAREIAKVDQHLASCDACRARQAELEDLGGSLRRALIPLPLGLAAFALNRWKLASS
                     IASVADRPATGVARVLGRIERPLLATSLGMASLGIVAAAVVGQRVPTSPAGGQLAAPP
                     GGGAAAPAPISSPPVVTFTDAVATVPAPAAPAAVATSGVAPAATQAAAASAVATAPAP
                     LPAPPGPPVTGSGGGTTDPTDPPPGGGTTNPTNPPSPPPPLASAGAALVAGPLQTTAA
                     VTADPSCTGTSTTGATAGSASVGGQTVGCSAPAPAPSSTTGAASATVTTGGSLTGTQT
                     VQVP"
     gene            complement(1104..1304)
                     /locus_tag="VF954_04595"
     CDS             complement(1104..1304)
                     /locus_tag="VF954_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX9530394.1"
                     /translation="MATGTVKWFNAEKGYGFISQSDGADVFVHFSAIQMNGYRSLEEG
                     QQVEFEVQEGPKGLQAASVRPV"
     gene            complement(1436..>2385)
                     /locus_tag="VF954_04600"
     CDS             complement(1436..>2385)
                     /locus_tag="VF954_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HEX9530395.1"
                     /translation="GVYLAAEQIPGYGLLALQSLGSMRSGTRAEVTEEKRSPVDFALW
                     KKAKPGEPEWQSPWGPGRPGWHTECVVMSVDKLGPEFDLHGGGLDLRFPHHENERAQA
                     VGMGFPFSRRWMHNGLVVDQDGRKMSKSEGNTLSLSDLLARYDGRAYRMLVLQSSYRS
                     PLRVGETGLRRAAETLVRLDTFAGRTAGLEPARPSLEVVRRFVERMDDDLNTPEAMAI
                     VFELVTEGNRALDAGQQSRASEAVASVRHLCAAVGLDLRARAVEIDAHAASLVAERDR
                     ARAAQDFGRADQLRADLMARGWMVEDGAEGTEVRRKPEL"
BASE COUNT          312 a          922 c          824 g          327 t
ORIGIN      
        1 aacggggcgg cccagctggc ggtgcgcgcc cgagccgggc tgcgggaacg ctttctccaa
       61 gcccatctgc agggccccgt cgaggagcag tgccgcttca cggtcgagcg cctgggggcg
      121 tacgtcggcg gcgggctggc cgcccgtgag atcgccaagg tcgaccagca cctggcctcc
      181 tgcgacgcgt gccgggcccg ccaggcggag ctcgaggacc tgggcggctc cctgcgccgg
      241 gcactgatcc cgctgcccct cgggctggcg gccttcgctc tcaaccggtg gaagctcgcc
      301 agctcgatcg cctccgtcgc cgatcgaccg gccacgggcg tggcccgggt gctcggccgg
      361 atcgagcgtc ctcttctcgc cacctcgttg ggcatggcga gcctcgggat cgtggcagcc
      421 gctgtcgtgg gccagcgggt gccgaccagc cccgccgggg ggcagcttgc cgcgcccccc
      481 ggcggcgggg cggcggcgcc ggcgccgatc tcgtcgccgc cggtggtcac cttcaccgat
      541 gccgtggcca cggtcccggc cccggccgcc ccggccgccg tagccacctc gggtgtggcg
      601 ccggccgcga cccaggcggc ggctgcgagc gctgtggcta ccgcgccggc gccccttccg
      661 gcccctccgg gccctccggt cacgggctcg ggtgggggca cgaccgaccc gaccgacccg
      721 cccccgggtg ggggcacgac caacccgacc aacccgccca gcccgccccc gcccctggcc
      781 agcgcgggcg cagccctcgt cgccggcccg ctccagacga cggcggctgt gaccgccgac
      841 ccgagctgca ccgggaccag caccacgggc gccaccgccg gctcggccag cgtcggaggc
      901 cagacggtgg gctgctcggc gccggctccc gcgccgtcgt cgaccacggg agcggcgagc
      961 gccacggtga ccactggcgg ctccctcacg gggacccaga ccgtccaagt gccctagccg
     1021 ccgggcaccg acccccggac ttcagcccgg acatgaagaa acggcgccca ggggcgccgt
     1081 ttcctcgcac gtggccgaac gaatcagacg ggacgaacgc tcgcagcctg caggcccttg
     1141 gggccctcct ggacctcgaa ctcgacctgc tggccctcct cgagggagcg gtagccattc
     1201 atctggatgg cggagaagtg gacgaacacg tccgctccgt ccgactggga gatgaatccg
     1261 tagcccttct ctgcgttgaa ccacttcacg gtgccggtcg ccattgtggc ggaccccctt
     1321 ctgctgactg actgaaacga ggggtgcctg cacgtgcgac tgcttgccga ccgattgaag
     1381 cgttctccct caaacgcgca ccgtaccagg gagggcctgc cgtcgtcagc cgggctcaga
     1441 gctcgggctt ccggcggact tcggtgcctt ccgcgccgtc ttccaccatc cagccccggg
     1501 ccatcaggtc ggcccggagc tggtcggccc gcccgaaatc ctgggcggcc cgggcacggt
     1561 cccgctcggc caccaggctc gcggcgtggg cgtcgatctc caccgcccgg gcccgcaggt
     1621 ccaggccgac cgcggcgcac aggtggcgca cggacgcgac cgcttcgctg gctctggact
     1681 gctgcccggc gtccagggcc cggttgccct cggtcaccag ttcaaagacg atggccatgg
     1741 cctcgggggt gttgagatcg tcgtccatcc gctccacgaa ccgccggacc acctcgagcg
     1801 acgggcgcgc cggctccaac ccggccgtgc gcccggcgaa ggtgtcgaga cgcaccaggg
     1861 tctcggcggc tcgccgcagc ccggtctcgc cgacgcgcaa cggggagcgg tagctcgact
     1921 gtaggaccag catgcggtag gcccgtccgt cgtagcgggc cagcagatcg ctgagcgaga
     1981 gagtgttgcc ctccgacttg ctcatcttgc ggccgtcctg gtcgaccacg aggccgttgt
     2041 gcatccatcg ccgggagaac ggaaatccca tgcccacggc ctgggcccgc tcgttctcgt
     2101 gatggggaaa gcggagatcc aacccgccgc cgtgcaggtc gaactctgga ccgagcttgt
     2161 cgaccgacat gacgacgcac tcggtgtgcc agcccggacg gcccggtccc cacggcgact
     2221 gccattccgg ctcccccggc ttggccttct tccagagggc gaagtccacg ggcgagcgct
     2281 tctcctccgt cacctcggcc ctggtgccgg agcgcatgga gccgagcgac tggagggcca
     2341 gcaggccgta gcccggtatc tgctccgccg ccaggtacac cccgt
//
LOCUS       DASWGG010000255         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1206305, whole genome shotgun sequence.
ACCESSION   DASWGG010000255 DASWGG010000000
VERSION     DASWGG010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206305"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VF954_04605"
     CDS             complement(<1..1220)
                     /locus_tag="VF954_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX9530396.1"
                     /translation="MTTSTVPWDELIEADRVHGSLYTDPAIYEAELERIWYRTWVYVG
                     HESEVPEPNDYVLKSIGPQPVIMTRDRQGDIHLLLNRCSHRANLVCDAPQGNSSAFRC
                     PYHGWTFANTGALLGYPFSSGYGGRELKSELGLAPVPRMERYRGFVFGSFAEDGPSLV
                     EHLGAAAGEIDRLVRLSPEGRIQLTAGWLQHKVQANWKMLLENETDGYHPQFVHASIF
                     AVADSGIGDLYGEKSTAVSRDLGGGHTENDLRPEFRRLGEPLRWFGTTAERLPDYVTR
                     MRAAYGDAAEEILVEGSPHVMIFPNLFIAEIQIFVLQPVAVDETVQHVTAIQFAGAPD
                     LNRRMLQQTLGSVGPAGFLLADDSEMYERNQRGVQVRRPEWLVLKRGLHRERVDERGH
                     LVGDATDEVPMRAM"
     gene            complement(1244..>1610)
                     /locus_tag="VF954_04610"
     CDS             complement(1244..>1610)
                     /locus_tag="VF954_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9530397.1"
                     /translation="MQQGGWGRIVNMSSEAGRYGGKADAVYSATKGAIIAFTRSIAME
                     NARYGVTVNAVAPGPIETPLLRRMDPRAIDMVAAGTLLRRLGEPDEVAAAVAFLASAE
                     ASYITGETLAVSGGMGLGA"
BASE COUNT          230 a          539 c          589 g          252 t
ORIGIN      
        1 cacatggccc gcatcggcac ctcgtcggtg gcgtcgccga cgaggtggcc ccgctcgtcg
       61 acccgttccc ggtgcaggcc ccgcttgagc accagccact cgggccggcg gacttgcacg
      121 ccccgctggt tgcgctcgta catctcggag tcgtcggcca ggaggaaccc ggccgggccg
      181 acggagccga gcgtctgctg cagcatgcgc cggttgaggt cgggggcgcc ggcgaactgg
      241 atggcggtca cgtgctggac ggtctcgtcc accgccaccg gctgcagcac gaagatctgg
      301 atctcggcga tgaacaggtt ggggaagatc atcacgtggg gcgagccctc gacgaggatc
      361 tcctcggcgg cgtcgccgta cgccgcccgc atcctcgtca cgtagtcggg caggcgctcg
      421 gcggtggtgc cgaaccagcg cagcggctcg ccgaggcgac ggaactccgg ccgcaggtcg
      481 ttctcggtgt ggccgccgcc gaggtcgcga ctcaccgccg tcgacttctc cccgtagagg
      541 tcgccgatgc cggagtcggc cacggcgaag atcgaggcgt ggacgaactg ggggtggtag
      601 ccgtcggtct cgttctccag cagcatcttc cagttggcct gcaccttgtg ctggagccag
      661 ccggcggtga gctggatgcg accttcgggg gagagccgga cgaggcggtc gatctccccg
      721 gcggcggcgc cgaggtgctc gaccaggctc ggcccgtcct cggcgaagga gccgaagacg
      781 aacccccggt agcgttccat ccggggcacg ggggccaggc ccagctccga cttgagctcc
      841 cggccgccgt agccggagct gaacgggtag ccgagcagcg ctccggtgtt ggcgaacgtc
      901 cacccgtggt acgggcaccg gaaggcactg gaattcccct ggggagcgtc gcagaccagg
      961 ttggcccggt gcgagcaccg gttgagcagc aggtggatgt cgccttggcg gtcccgggtc
     1021 atgatcaccg gctgggggcc gatcgacttg aggacgtagt cgttcggttc ggggacctcg
     1081 ctctcgtggc cgacgtagac ccacgtccgg taccagatgc gctccagttc cgcctcgtag
     1141 atggccgggt cggtatagag ggatccgtgg acccgatcgg cctcgatcag ctcatcccag
     1201 ggaacggtcg aggtggtcat gctcgctctc tcctctttag cgttcaggcg cccagcccca
     1261 tgccgccgga gacggccagg gtctcgccgg tgatgtacga cgcctccgcc gacgcgagga
     1321 aggccacggc ggcggccact tcgtcaggtt caccgaggcg gcgcaacagc gtgccggcgg
     1381 caaccatgtc gatggcccgg ggatccatgc gccgcaggag cggcgtctcg atcgggccgg
     1441 gggcgacggc gttgacggtg acgccgtagc gggcgttctc catggcgatc gaccgggtga
     1501 aggcgatgat ggcgcccttg gtggccgagt agacggcgtc ggccttgccg ccgtaccggc
     1561 cggcctccga cgacatgttc acgatgcggc cccagccgcc ctgctgcatg
//
LOCUS       DASWGG010000256         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3134585, whole genome shotgun sequence.
ACCESSION   DASWGG010000256 DASWGG010000000
VERSION     DASWGG010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3134585"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1476)
                     /locus_tag="VF954_04615"
     CDS             complement(172..1476)
                     /locus_tag="VF954_04615"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX9530398.1"
                     /translation="MTTTVGIGVLGCGNVGGALVSLLAADAAFIEARTGLRLDVRRVA
                     VRNTAKERTIELPPGCLTHDAESVVNDAGVEVVVEVIGGIEPARSLILDALKAGKPVV
                     TANKELLANVGAELFQAAEAAGVDLLYEAAVAGGIPLMRPLRESLAGERITRVMGIVN
                     GTTNYILTRMTEAGASYADALAEAQGLGFAERDPTADVEGFDAGAKAAIIATIAFGQQ
                     VVAGDVYREGISGITAADIAFATRLGYVVKLLAIAERSEPDDAADQDAVAVRVHPAMI
                     PRAHPLASVRESFNAVFIEGQAVGDLMLYGRGAGGLPTASAVVGDLLDAAHNLVSGGR
                     GRVGSLARANLRPMDELSSQYYLNLEVVDRPGVLAAVAEVFGRHGVSIRSMEQEGLGD
                     EARLVFITHRARERDMQATMHDLRHLASVERVGGLLRVMGPE"
     gene            complement(1500..>1712)
                     /locus_tag="VF954_04620"
     CDS             complement(1500..>1712)
                     /locus_tag="VF954_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX9530399.1"
                     /translation="SGDVVVRDACVPASLAPGDLLATPVTGAYGHSMASTYNKVPRPA
                     VLFVRRGQVRVVVRRETLDDLLRLDC"
BASE COUNT          235 a          645 c          598 g          234 t
ORIGIN      
        1 tgagcccttc gtacttgagc cacacgtcgg cgcccacctg cgtcgagagg cgcggcgccg
       61 ggagcagggg cgtgccaccc tcctgcaacg tgaccacggg ggtggccccc cggacgggga
      121 ggaagtcgcg gtactcctcg atgaggcccc gccaccccgg ccgggccatc cctattcggg
      181 ccccatgacc cgcagcaggc cgccgacgcg ttcgacggag gcgaggtggc gcaggtcgtg
      241 catcgtcgcc tgcatgtccc gctcgcgggc ccggtgggtg atgaagacca ggcgggcctc
      301 gtcccccagg ccctcctgct ccatggaacg gatggagacc ccgtgccggc cgaacacctc
      361 ggcgactgcc gccagcacgc ccggccggtc caccacctcg aggttgaggt agtactgcga
      421 gctgagctcg tccatgggcc ggaggttggc ccgggccagc gatccgacgc ggccccgccc
      481 ccccgagacc aggttgtggg cggcgtccag gaggtcgccc accactgcgc tcgccgtggg
      541 cagcccgccc gctccccgcc cgtacaacat caagtccccc accgcctggc cctctatgaa
      601 caccgcgttg aacgactcgc gaaccgacgc caggggatgc gccctcggga tcatggcggg
      661 gtgcactcgc acggccaccg cgtcctggtc ggctgcgtcg tcgggctcgc tccgctcggc
      721 gatggcgagc agcttcacca cgtagccgag ccgggtggcg aaggcgatgt cggcggcggt
      781 gatcccgctg atcccctcgc ggtagacgtc gccggccacc acctgctggc cgaacgcgat
      841 ggtcgcgatg atcgccgcct tggcgccggc gtcgaagccc tcgacgtccg cggtcgggtc
      901 gcgctcggcg aagcccaggc cctgggcctc ggcgagcgca tcggcgtagc tcgcgcccgc
      961 ctcggtcatc cgcgtgagga tgtagttggt ggtgccgttg acgatcccca tcacgcgcgt
     1021 gatgcgctcc cccgccagtg actcgcgcag cggccgcatc agcggtatgc cgcctgcgac
     1081 cgcggcctcg tagagcaggt cgaccccggc ggcctcggcc gcctggaaca gctcggcgcc
     1141 cacgttggcg agcagctcct tgttggccgt gaccaccggc ttgcccgcct tgagggcgtc
     1201 caggatgagc gatcgagccg gctcgatccc gccgatgacc tcgaccacga cctcgacccc
     1261 ggcgtcgttg accacggact cggcgtcatg ggtcaggcag ccggggggga gctcgatggt
     1321 gcgttccttg gccgtgttgc gcaccgccac ccgcctgacg tcgaggcgca ggccggtgcg
     1381 ggcctcgatg aaggcggcgt cggccgcgag caggctgacc agggccccgc ccacgttgcc
     1441 gcagcccagc accccgatcc cgacggtcgt cgtcacggca tgcagactag gccgtgggct
     1501 cagcagtcga ggcggaggag gtcgtcgagc gtctcgcgcc gcaccaccac cctcacctgc
     1561 ccacggcgca cgaacagcac cgccgggcgg ggcaccttgt tgtacgtgga cgccatcgag
     1621 tggccatagg cgccggtcac cggcgtggcc agcaggtcac cgggggccag gctcgccggc
     1681 acgcacgcgt cgcggacgac gacgtcgccc ga
//
LOCUS       DASWGG010000257         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2802853, whole genome shotgun sequence.
ACCESSION   DASWGG010000257 DASWGG010000000
VERSION     DASWGG010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2802853"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="VF954_04625"
     CDS             complement(<1..1605)
                     /locus_tag="VF954_04625"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX9530400.1"
                     /translation="MPGPDAFRLPRTVEPVRYELTITPDVQSSTFSGEERIRLRVAEP
                     VDRIVLNAAELAVTAAELVPAAGGPGTGQRVLVAHDDAAERVTLSPNEPLGPGTWDLL
                     LRFDGRLNDKLRGFYRSTFKDDAGTEHVIATTQFEATDARRAFPCWDEPDCKAVFSVA
                     LVVDPGLTAVSNGRVVAEEVRPDGRRRITFEDTMPMSTYLVAFVVGALSATAATDVDG
                     VPVRVVHVAGKEHLTGFAREVAAHALRFFTRWFGLPYPSGKLDHIAIPDFAFGAMENL
                     GAVVYRESVLLIDPETATRDEMERVAEVIAHETAHMWFGDLVTMRWWNGIWLNEAFAT
                     FMEMLCVDAFRPEWERWSTFAVSREGAMAIDGLGSTRPIEFPVGQPDEAEAMFDVLTY
                     QKGAGVLRMLERYLGAEAFRQGIGRYLEAHAHGNTETRDLWDALEGSSGQPARAIMDS
                     WILQGGHPLVTARLAADGLHLRLTQRRFRYLGGGYLGGGHPGGGQDPPDEVWQIPLRM
                     RLGAADDDRDDREVRLLMTGAAIDVEL"
BASE COUNT          234 a          587 c          591 g          226 t
ORIGIN      
        1 gagctcgacg tcgatggcgg ccccggtcat cagcagccgt acctcgcgat cgtcgcgatc
       61 gtcgtccgcg gcccccagcc gcatgcgcaa cgggatctgc cacacctcgt cgggcgggtc
      121 ctggccgcca cccgggtggc cgccacccag gtagccgcca cccaggtacc ggaagcgccg
      181 ctgggtgagc cgcaggtgca gcccgtcggc cgccagccgg gccgtgacca gcgggtggcc
      241 gccctggagg atccagctgt ccatgatggc ccgggcgggc tggccggagg acccctccag
      301 ggcatcccag aggtcacggg tctcggtgtt gccgtgggcg tgggcctcca ggtagcggcc
      361 gatcccctgc cggaacgcct ccgccccgag gtagcgctcg agcatgcgca gcactcccgc
      421 ccccttctgg taggtcagca cgtcgaacat ggcctcggcc tcgtccggtt ggccgaccgg
      481 gaactcgatg ggcctggtcg agcccagccc gtcgatcgcc atggcgccct cccggctcac
      541 ggcgaaggtc gaccaccgtt cccactcggg ccggaaggcg tccacgcaga gcatctccat
      601 gaaggtggcg aaggcctcgt tgagccagat cccgttccac cacctcatgg tcaccaggtc
      661 gccgaaccac atgtgggccg tctcgtgggc gatgacctcg gccacccgct ccatctcgtc
      721 gcgggtggcc gtctccgggt cgatcagcag gaccgactcc cggtagacga cggcgcccag
      781 gttctccatg gcgccgaagg cgaagtcggg gatggcgatg tggtcgagct tgcccgacgg
      841 gtaggggagg ccgaaccatc gggtgaagaa ccgcagggcg tgcgccgcca cctcgcgcgc
      901 gaaccccgtc aggtgctcct tgccggcgac gtggacgacc cggacgggca cgccgtcgac
      961 gtcggtggcc gctgtggccg agagcgcccc caccacgaac gccaccaggt aggtggacat
     1021 cggcatggtg tcctcgaagg tgatgcgccg ccgcccgtcc ggtcgcacct cctcggccac
     1081 gacccggccg ttggacaccg ccgtgagccc cgggtccacg accagcgcca cggagaacac
     1141 ggccttgcag tcgggctcgt cccagcacgg gaaggcccgg cgggcgtcgg tggcctcgaa
     1201 ctgggtggtg gcgatcacgt gctcggtgcc ggcgtcgtcc ttgaaggtcg accggtagaa
     1261 gccccggagc ttgtcgttga gccggccgtc gaagcgaagc aggaggtccc acgtgcccgg
     1321 gccgagcggc tcattgggag aaagggtgac cctctcggct gcgtcgtcgt gagccacgag
     1381 cacgcgctgg cccgtcccgg gcccgccggc ggcggggacg agctcggcgg cggtcacggc
     1441 cagctcggcc gcgttgagca cgatccggtc caccggctcg gcgacgcgga gccggatgcg
     1501 ctcctccccg gagaaggtgg agctctgcac gtcgggcgtg atggtcagct cgtagcggac
     1561 gggctccacc gtccggggaa ggcggaaggc atcggggccc ggcatggacg gggtagccta
     1621 ccggcccggc tacggtct
//
LOCUS       DASWGG010000258         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2048251, whole genome shotgun sequence.
ACCESSION   DASWGG010000258 DASWGG010000000
VERSION     DASWGG010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048251"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VF954_04630"
     CDS             <1..618
                     /locus_tag="VF954_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530401.1"
                     /translation="GDGTVSVLGGPGPLTNAALGHDDDALLAVGLLGAGPGWSVVLAG
                     PAPGTTGGVGSGRRDLVSLVSRRVKLALLQLAIAFVVVVLWRSRRLGRVASEPQPVQI
                     EASELVAAVGNLLHQGRRRDEAARLLRADLARRLTARLGLPGRPSADRLADAVAERTG
                     LDPALVLRALEPGPVPHDDALVALARAADEIEAELARAGEVAHAG"
     gene            608..>1567
                     /locus_tag="VF954_04635"
     CDS             608..>1567
                     /locus_tag="VF954_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9530402.1"
                     /translation="MPAEPRTRRAAPDETDPRAAVQAVREEVAKVVVGQDAVLTGVVS
                     ALLVGGHVLLEGVPGVAKTLLVKAVAAAHALEFKRIQFTPDLMPSDVTGQLVLDAGAG
                     RTGAADFRFRPGPVFTNLLLADEINRTPPRTQAALLEAMEEAQVSVEGAPRALPSPFC
                     VVATQNPIEYEGTYPLPEAQLDRFLFKLLVGYPSAEQEAEVLARHDEGLDPHDMAAAG
                     VRAVAGAADLAAARREVGALRVDPVVRAYMVAIARATRQSPSLRLGVSPRGATMLLHA
                     AKAWAWLSGRDFVTPDEVKAVVKPTFRHRLHVRPELELEGTTAD"
BASE COUNT          175 a          612 c          580 g          200 t
ORIGIN      
        1 ggagacggca ccgtgtccgt gctcggtggg ccgggcccgc tcaccaacgc cgccctcggc
       61 cacgacgacg acgccctgct cgccgtcggg ctgctcgggg ccggcccggg gtggtcggtc
      121 gtcctcgccg gcccggcccc cggaaccacc ggcggagtcg gcagcggccg ccgcgacctg
      181 gtgagcctgg tgtcccgccg ggtgaagctg gcgctgctcc agctcgccat cgccttcgtc
      241 gtcgtggtgc tgtggcggtc ccggcggctc gggcgggtcg cgtccgagcc ccaacccgtg
      301 cagatcgagg cctccgagct ggtcgcggcc gtgggcaacc tgctccacca gggccggcgg
      361 cgggacgagg cggcgcggct gctgcgggcg gatctggctc ggcggctcac cgctcgcctg
      421 ggcctgccgg gccgcccgag cgccgaccgg ctcgccgatg ccgtcgccga gcgcacgggt
      481 ttggaccccg cgctggtcct gcgcgcacta gaacctgggc cggtgcccca cgacgacgcc
      541 ctggtggccc tggcccgagc ggccgacgag atcgaagccg agctggcccg cgcgggcgag
      601 gtggcccatg ccggctgagc cgcgcacgcg ccgggcggca ccggacgaga ccgacccccg
      661 cgccgccgtg caggccgtcc gggaggaggt ggccaaggtc gtggtgggcc aggacgccgt
      721 gctgaccggg gtggtgtcgg cgctgctggt cggcggtcac gtcctgctcg aaggcgtccc
      781 cggcgtggcc aagactctcc tcgtcaaggc cgtggccgcg gcccacgccc tcgagttcaa
      841 gcgcatccag ttcacgcccg acctcatgcc ctccgacgtg accggccagc tcgtcctcga
      901 cgccggcgcc ggccgcaccg gggccgcgga cttccgcttc cgcccgggcc ccgttttcac
      961 caaccttctc ctcgccgacg agatcaaccg gacgccgccc cggacgcagg cggccctcct
     1021 cgaggccatg gaggaggccc aggtctccgt ggagggggcg ccccgcgcgc tgcccagccc
     1081 cttctgcgtg gtggcgacgc agaacccgat cgagtacgag ggcacctacc cgctgccaga
     1141 ggcccagctc gaccggttcc tcttcaagtt gctcgtcggg taccccagtg ccgagcagga
     1201 ggcggaggtg ctcgcccgcc acgacgaggg cctcgatccc cacgacatgg ccgcggccgg
     1261 ggtgcgggcc gtcgccggcg ccgccgacct ggccgcggcc cggcgcgagg tgggcgccct
     1321 ccgcgtcgat cccgtcgtac gcgcctacat ggtggccatc gcccgcgcca cccggcagtc
     1381 gccgtcgctg cggctggggg tctcgccccg gggcgccacc atgctgctgc acgcggccaa
     1441 ggcctgggcg tggctgtcgg gacgggactt cgtgacgccg gacgaggtga aggccgtggt
     1501 caagccgacc ttccgccacc gcctgcacgt gcggcccgag ctcgagctgg agggcaccac
     1561 cgcggac
//
LOCUS       DASWGG010000259         3324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3135536, whole genome shotgun sequence.
ACCESSION   DASWGG010000259 DASWGG010000000
VERSION     DASWGG010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3135536"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VF954_04640"
     CDS             complement(<1..381)
                     /locus_tag="VF954_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530403.1"
                     /translation="MIRPIRRGVMVLLLLLVAEYLVLPQLAGARRSAHLLTRVNGLYL
                     GAGVLLELSALVAYAELTRAVLLDGHRLSLWTLFRIDLSTLAVSHMVPGGNAAGTSLG
                     YRLLTEHGVSGADAGFGLATQGMGS"
     gene            complement(454..1245)
                     /locus_tag="VF954_04645"
     CDS             complement(454..1245)
                     /locus_tag="VF954_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9530404.1"
                     /translation="MHAPALRLTDVVLRLGAADVLLDVNWTVGPGERWVVLGPNGSGK
                     TSLLRLAAGYLHPTEGTVEILGATLGRVDVRRLRTRIGVTSAAMTKLLRPSITALDVV
                     MTGKEAALEAWWHEWSDADRERARGLLAGAGFGYAAGRKWDALSEGERQQVQLARTLM
                     ADPELLLLDEPNAGLDLGGRERLVRHLGALAADAASPPIVLVTHHVEEIPRAFTHALL
                     LRSGRVLAAGDVDHVLTDAALSEAFELKLRVQRQDGRFSCQAVAE"
     gene            1342..2580
                     /locus_tag="VF954_04650"
     CDS             1342..2580
                     /locus_tag="VF954_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9530405.1"
                     /translation="MAEDSAPTPTPARVPLRTILALDALVLAVVLGLYLAYHLRRVLV
                     LLAISTFLAVVLSPAVDLLHRKGIRRSLATSMVFFGGMASLAGLGYLFVRPIYRAGRH
                     FADDLPSLVRQAQSGQGRIGELIRRYHVEKFVNDNAPKLSNALSHAGGPALALLQSLI
                     SGLAGLFLIAVLTFLILLEGPALSSAALRLLPAAQAGRVRRVARDVSRSVTGYVIGNL
                     ATSLIAGAVIYVTLLIVGVPFAFLFGLWVAMVDLLPLVGGLLAGVPTVAIAFLHSPEA
                     GFVTLVVFLVYQQLENHILNPLIMSRTVRLNPLWVMLSVLVGADLGGIVGALLAIPAA
                     GAIQVVARDVWDTRQGRLKPEPTVGEDGAAYQRDPAPTPDTNGKPGVELRHPDEAVVA
                     QRADVEPVPGEDLGQAREGA"
     gene            complement(2488..3234)
                     /locus_tag="VF954_04655"
     CDS             complement(2488..3234)
                     /locus_tag="VF954_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9530406.1"
                     /translation="MRGGPRSATIGVATGLAAVAVAAQSLPVGATSLRALRVRAFPRL
                     AGLGRRDHVALTFDDGPDPASTPAFLEALAGLGWHATFFMLGSMVRKAPGLAAEVAAA
                     GHEVAVHGDEHRSQLWRTPGAVAADLDRARHEVAGATGSAPRWFRPPYGVLTLGGVQA
                     ARGAGLSPVLWTAWGRDWRAEATPATVAGDVARDLGPGATVLLHDSDCTSAPGAWRSA
                     LGALPRLAEVFAWHGLDVGPLRDHGLVGVA"
BASE COUNT          432 a         1240 c         1212 g          440 t
ORIGIN      
        1 cgagcccatg ccctgggtgg ccaggccgaa gccggcgtcg gcgccggaca caccgtgctc
       61 ggtcagcagg cggtatccga ggctggtccc ggcggcgttg cccccaggga ccatgtggct
      121 gaccgccagg gtggacaggt cgatccgaaa gagcgtccac agcgagagac ggtggccgtc
      181 cagcagcaca gcgcgggtca gctcggcgta cgccaccagg gcgctgagct ccagcagcac
      241 gccggccccc aggtacaggc cgttcaccct ggtcaggagg tgagccgagc gacgggcccc
      301 ggccagctgc ggcaggacca ggtactcggc cacgagaagg agcagcaaca ccatcacgcc
      361 ccggcggatc ggccgtatca cggaccagcg ccgggggccc gtcgtggccg ggccgtcatc
      421 catcggcgcg gcgatcggcg agcggcgcgg tcgtcattcg gcgaccgcct ggcacgagaa
      481 gcggccatct tggcgctgga ctcgcagctt gagctcgaat gcctcgctga gcgcggcgtc
      541 ggtgagcacg tggtcgacgt cgccggcggc cagcacccgc cccgaccgga gcagcagggc
      601 gtgggtgaag gctcggggga tctcctccac gtggtgggtc accaggacga tcggtggtga
      661 cgccgcgtcc gccgccagcg cgccgaggtg gcgcaccagc cgctcgcggc ccccgaggtc
      721 gagaccggcg ttgggctcgt cgaggaggag cagctcgggg tcggccatga gggtacgggc
      781 cagctggacc tgctggcgct ccccttcgga gagcgcatcc catttccgcc cggcggcgta
      841 cccgaacccg gcgccggcca gcagcccgcg ggcccgctcc cggtcggcgt ctgaccactc
      901 gtgccaccat gcctcgaggg cggcctcctt gccggtcatc accacgtcga gggccgtgat
      961 cgacggccgg aggagcttgg tcatggccgc gctggtcacg ccgatgcggg tgcgcaggcg
     1021 gcggacgtcg acgcggccca gggtggcgcc gaggatctcg accgtgcctt cggtgggatg
     1081 gagatagcca gccgccagcc ggagcaagga cgtcttgccc gacccgttgg ggccgaggac
     1141 gacccagcgc tcgccgggcc ccacggtcca gttgacgtcg agtagcacgt cagccgcccc
     1201 gagccgcagg accacgtcgg tcaggcgcag ggcgggcgcg tgcatggctg gaccttagag
     1261 gcccgtcacc tgctgccgtg ggccgatcag tcgccagcgg gatcatgggt ggcgtcgagg
     1321 gcggggcata gcctgagccc gatggccgag gacagcgcgc cgaccccgac ccctgcccgg
     1381 gtcccgctgc gcaccatcct ggccctggac gccctcgtgc tcgccgtcgt cctcggtctg
     1441 tacctcgcct atcacctccg ccgggtcctc gtgctgctgg cgatctcgac gttcctggcc
     1501 gtcgtgctca gcccggcagt ggacctcctc caccgcaagg gcattcggcg gagcctggcg
     1561 acgagcatgg tcttcttcgg cggcatggcg tccctcgccg gcctgggcta cctcttcgtc
     1621 cgcccgatct accgcgccgg gcggcacttc gccgacgacc tgccgtcgct ggtccggcag
     1681 gcccagagcg gtcagggccg gatcggcgag ctgatccgcc ggtaccacgt cgagaagttc
     1741 gtgaacgaca acgcccccaa gctgagcaac gccctgagcc acgccggcgg gccggccctg
     1801 gccctgctcc agtcgctgat ctccgggctg gccgggttgt tcctcatcgc cgtgctgacc
     1861 ttcctgatcc tgctcgaagg cccggcgctg tcgagcgccg cgctccggct gctgcccgcg
     1921 gcgcaggccg ggcgcgtccg gcgggtggcc agggacgtct cccggtcggt aaccggctac
     1981 gtgatcggca acctggccac gtcgctcatc gccggcgcgg tgatctacgt cacgctgctg
     2041 atcgtcgggg tgcccttcgc gttcctgttc gggctgtggg tcgccatggt cgatctgctg
     2101 ccgctcgtgg gcgggctgct ggccggcgtc ccgacggtgg ccatcgcgtt cctgcattcg
     2161 cccgaggccg ggttcgtgac gctcgtggtc ttcctcgtct accagcagct cgagaaccac
     2221 atcctcaacc cgctcatcat gagccggacc gtccggctca acccgctctg ggtcatgctg
     2281 tcggtgctgg tcggcgccga cctcggcggc atcgtcggcg ccctgctggc catcccggcc
     2341 gccggcgcca tccaggtcgt ggcccgcgac gtgtgggaca cccgccaagg gcggttgaag
     2401 cccgagccca ccgtcggcga ggacggcgcg gcctaccaga gggacccggc cccgaccccg
     2461 gacaccaacg gcaagcccgg ggtggagcta cgccaccccg acgaggccgt ggtcgcgcaa
     2521 cgggccgacg tcgagcccgt gccaggcgaa gacctcggcc aggcgcggga gggcgcctag
     2581 ggccgatcgc caggcgcccg gcgccgaggt gcagtccgag tcgtggagca gcacggtcgc
     2641 tccgggtccg aggtcgcggg cgacgtcgcc cgcgacggtg gccggagtgg cctcggcccg
     2701 ccagtcccgg ccccacgccg tccacagcac cgggctcagc ccggccccgc gggcggcctg
     2761 caccccgccg agggtgagca cgccgtaggg ggggcggaac cagcgcggcg cgctgccggt
     2821 ggccccggcc acctcgtggc gcgctcggtc aaggtctgcg gcgaccgcac ctggcgtgcg
     2881 ccacagctgc gagcggtgct cgtcgccgtg caccgcaacc tcgtgaccgg cggccgcgac
     2941 ctcggctgcc agcccgggcg cctttcggac catggacccg agcatgaaga acgtcgcgtg
     3001 ccacccgagc ccggccaggg cctcgaggaa ggccggcgtc gaagccgggt cggggccgtc
     3061 gtcgaaggtc agggccacgt ggtcccggcg tcccagcccg gccaggcggg gaaaggcccg
     3121 cacccgcagg gcccgcaggc tggtcgcgcc cacggggagg ctctgggcgg ccaccgccac
     3181 cgccgccagc ccggtggcca cgccgatcgt ggccgaccgc ggcccgcccc tcacgtcaac
     3241 tgcgcaagcg gcgtcggtgc gaggtcggct tgcgccagcg ctgcctgcag ggcggtcagc
     3301 ccggcgtcca acgaagggcc cccg
//
LOCUS       DASWGG010000260         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3136157, whole genome shotgun sequence.
ACCESSION   DASWGG010000260 DASWGG010000000
VERSION     DASWGG010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3136157"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VF954_04660"
     CDS             <1..311
                     /locus_tag="VF954_04660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9530407.1"
                     /translation="VDPAWHRHKIATRLMLNSARVALRRGCRHLTLEVRLGNAGAQAL
                     YHLFGFHPAGIRRNYYVETNEDALVMWADDIDTSAYTRRLAGIEVCVPGTTTVEEIKP
                     "
     gene            308..1318
                     /gene="tsaD"
                     /locus_tag="VF954_04665"
     CDS             308..1318
                     /gene="tsaD"
                     /locus_tag="VF954_04665"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX9530408.1"
                     /translation="MTPRQLVLGIETSCDETAAAVVADGHDVLSSVVSSQVDVHARYG
                     GVVPEIASRAHVELLTPVVAEAMVEAGVDGAAVAAVAATAGPGLIGSLLVGVSAAKAM
                     ALVWGVPFVGVNHLEGHLYASFLEEPDLTPPLVMLLVSGGHTMLVTMEGPGAYRLLGQ
                     TIDDAAGEAFDKVARFLGLGYPGGPAIDRVAMDGDPAAIAFPRSLMGEGYDFSFSGLK
                     TSVITYVRKHPDATVQDVAASFQEAVVDVLVGKARAAAREVGATGICLAGGVAANSVL
                     RERTIDACIADGLRPFVPSRSMCTDNAAMIAAAGYWQLQSHGPSPLDLGADPNLRLPL
                     LG"
     gene            1535..>1664
                     /locus_tag="VF954_04670"
     CDS             1535..>1664
                     /locus_tag="VF954_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX9530409.1"
                     /translation="MNLQPLDDRIVVRPGESEETTASGLVIPDTAKEKPQQGEVLAV"
BASE COUNT          237 a          608 c          585 g          234 t
ORIGIN      
        1 ccgtcgaccc cgcctggcac cgccacaaga tcgccacgcg gctcatgctc aacagcgccc
       61 gggtggccct ccggcggggc tgccggcacc tcaccctcga ggtccggctc ggcaacgccg
      121 gcgcccaggc gctgtaccac ctcttcgggt tccaccccgc cggcatccgc aggaactact
      181 acgtcgagac caacgaggac gccctggtca tgtgggccga cgacatcgac acctcggcgt
      241 acacccgccg cctggccggc atcgaggtgt gcgtgccggg caccaccacc gtcgaggaga
      301 tcaagccgtg acgccccgcc agctcgtgtt ggggatcgag acgtcctgcg acgagaccgc
      361 cgcggccgtg gtggcggacg gccacgacgt cctgtcctcg gtcgtcagca gccaggtcga
      421 cgtccacgcc cgctacggcg gggtggtgcc cgagatcgcc agccgggccc acgtcgagct
      481 gctgaccccg gtcgtcgccg aggccatggt cgaagccggc gtcgacggcg cagccgtggc
      541 ggcagtggcc gcgaccgcgg ggcccggcct catcgggtcg ctcctcgtgg gcgtgagcgc
      601 cgccaaggcc atggcgctgg tgtggggcgt gccgttcgtg ggcgtcaacc acctcgaggg
      661 ccacctgtac gccagcttcc tggaggagcc cgacctcacg ccgccgctgg tgatgctcct
      721 ggtctcgggc ggccacacca tgctcgtgac catggagggc ccgggcgcgt accgcctgct
      781 gggccagacc atcgacgacg ccgcggggga ggcgttcgac aaggtggccc gcttcctcgg
      841 gctcgggtac ccgggcgggc cggccatcga ccgggtggcc atggacgggg accccgccgc
      901 catcgccttc ccgaggtcgc tgatggggga ggggtacgac ttctccttca gcgggctcaa
      961 gacctcggtg atcacctacg tgcgaaagca tcccgacgcc accgtgcagg acgtggccgc
     1021 cagcttccag gaggcggtcg tcgacgtgct cgtgggcaag gcccgggccg cggcccggga
     1081 ggtgggcgcc accgggatct gcctggccgg cggggtggcg gccaactccg tgctccgcga
     1141 gaggacgatc gacgcgtgca tcgccgacgg gctgcggccg ttcgtgccca gccggtccat
     1201 gtgcaccgac aacgccgcca tgatcgcggc ggcggggtac tggcagctcc agtcccacgg
     1261 gccgagcccc ctcgacctcg gggcggaccc caacctccgc ctgcccctgc tggggtaggg
     1321 cccggccggg ccgggctcac cccggcggcg caaccgctgg gctcgtttcg ggaaccagcc
     1381 attctgttga cgccaatcgc gctatagccg tcattggcgt cgacagaaac ctgcgggccc
     1441 cgccgaggtt cgcggttgcc gactcgaatg ggggccgcta tctttagcac tcgcaaggtc
     1501 tgagtgctaa cgagtgccac aggaggcagc tctgatgaac ctgcagcccc tcgatgatcg
     1561 gatcgtcgtt cgtcccggcg agtccgagga aaccaccgcg tcgggactcg tgatcccgga
     1621 caccgccaag gagaagcccc agcagggcga ggtcctggcc gtcg
//
LOCUS       DASWGG010000261         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3136728, whole genome shotgun sequence.
ACCESSION   DASWGG010000261 DASWGG010000000
VERSION     DASWGG010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3136728"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VF954_04675"
     CDS             <1..625
                     /locus_tag="VF954_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9530410.1"
                     /translation="RPYEIGDDLRRVHWPSSAHTGDLMIRQDEMPWQGRATVVVDLRH
                     PVHSGESLELVISAAASVVTASWQRRALVRLVTTAGTDTGFAAGHDHVEAILERLAGA
                     SSTSAATMTAVLASLHRHGGGGAVVAITTAGASHGDLQAIARLRARYGSVTLVMFERA
                     AYAPGSPAANQPPRRVPSVSRLVRVDAARSFPTAWSDEMARPAGRRA"
     gene            622..>1556
                     /locus_tag="VF954_04680"
     CDS             622..>1556
                     /locus_tag="VF954_04680"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEX9530411.1"
                     /translation="MNTTGASAPRTLATTASLAVLSVGAAVGMGRLFTGTSWILPVLG
                     AAVAAHAVAWIARRADLTPAAAVAVSAAGVLLYAAWVVVPASTVYGLPWDGTLHTAAR
                     ILRQAWAAYPNISPPTRPLPGFMLATTVAFGTLAVLADFAAFRIKATIEACIPAFGLF
                     VFATALDRAHRHRATYAAVFLVPLVAFVLLHQADLRAGGSAWFASRARGGPIALLQGG
                     AAIGSAAVLIAVAAGPLLPTARQPGVYSYHHYSASPGRAGGVVVVSPLVDIRDGLVER
                     SAIELFTVRSTEPEYWRLTSLDAFDGTIWSANESYR"
BASE COUNT          168 a          617 c          534 g          237 t
ORIGIN      
        1 gcggccctac gagattggcg acgacctgcg ccgggtgcac tggccgagct cggcccacac
       61 cggcgatctg atgatccgcc aggacgagat gccctggcag ggccgggcca ccgtcgtggt
      121 cgacctgcgc catcccgtgc attccggcga gtcgctggag ctggtgatct cggccgcggc
      181 gagcgtggtc accgcgtcct ggcagcggcg ggccctggtc cggctggtga cgaccgccgg
      241 cacggatacc ggcttcgccg ccggccacga ccatgtcgag gccatcctcg agaggctcgc
      301 cggcgcctcg tcaacctcgg cggccaccat gaccgcggtc ctcgccagcc tgcaccgcca
      361 cggcgggggc ggggccgtgg tggccatcac caccgcgggc gcctcccatg gcgatctcca
      421 agccatcgcc cgcctccgag ccaggtacgg ctcggtgacc ctggtcatgt tcgagcgcgc
      481 cgcctacgct ccaggctccc ccgccgccaa ccagcccccg cgccgcgtcc cctctgtgtc
      541 acgcctcgtg cgcgtcgacg ccgcccggtc gttcccgacc gcctggtccg acgagatggc
      601 ccggcctgcc ggcaggcggg cgtgaacacg accggcgcgt ctgcgccccg caccctggcc
      661 accacggcct cgctggccgt cctctccgtc ggcgcggccg tcgggatggg gcggctgttc
      721 accggcacct cgtggatact ccccgtcctg ggcgcggcgg tggccgccca tgccgtggcc
      781 tggatcgccc ggcgggccga cctgacacct gcggccgcgg tcgccgtgtc cgccgcgggc
      841 gtcctgctgt acgccgcgtg ggtcgtggtc ccggcctcca ccgtctacgg gctgccgtgg
      901 gacggcaccc tgcacaccgc cgccaggatc ttgcggcagg cctgggcggc gtatcccaac
      961 atctcgccgc cgacgcggcc cctccctggg ttcatgctgg cgaccacggt cgcgttcggc
     1021 accttggccg tgctggcgga ctttgccgcc ttccggatca aggcgaccat cgaggcgtgc
     1081 atcccggcct tcgggttgtt cgtcttcgct acggcgctgg accgggccca ccgtcaccgg
     1141 gcgacgtatg ccgccgtgtt cctcgtcccc ctggtcgcgt tcgtcctgct ccatcaggcc
     1201 gacctgcgcg ccggtggctc ggcgtggttc gcgagccggg cccggggcgg cccgatcgcc
     1261 ctgctccagg gcggcgcggc catcggatcg gcagccgtgc tcatcgcggt ggccgccggt
     1321 cccctcctgc ccaccgcccg ccagcccggc gtctacagct accaccacta ctccgccagc
     1381 ccgggcaggg ccggcggagt agtggtggtg agcccgctgg tggacatccg cgacgggctg
     1441 gtggagcggt ccgcgatcga gctcttcacg gtcaggagca cggagccgga gtactggcgc
     1501 ctgacctccc tcgacgcctt cgacggcacc atctggtcgg ccaacgagtc gtaccg
//
LOCUS       DASWGG010000262         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3138068, whole genome shotgun sequence.
ACCESSION   DASWGG010000262 DASWGG010000000
VERSION     DASWGG010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3138068"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="VF954_04685"
     CDS             <1..1485
                     /locus_tag="VF954_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9530412.1"
                     /translation="WPVEPEGAPGPVPARVIDVGYRYREGRDLALDGVSMEVEVGQYV
                     AIVGPNGSGKSTLGRVVAGLPPTAGAVARPGPAGLGRLGGTAMVFQRPESQVLGARVK
                     DDIRWGLPAGAGMDTGTALEAVGLAGLADRETATLSGGELQRVALAAALARRPALLVA
                     DEATAMLDADGRRDVVATLHSLVGQGLTVIAITHHLDEVGPEDVLVRLDAGRVVSGAV
                     TGAGSGGANGRPAGSATGSATGDARGAGEATGPGATPDARPGSPEGRSLRLRGVGYVH
                     AWRSPWAHRALEGIDLDVAPGEAVLVVGPNGAGKTTLGWVVAGLVPPTEGTVRFGPGP
                     PSPGDVGIGFQHARLQLFRPTVEEDVGLGGAAPADVDTALRSVALDPDAYRHRLIDAL
                     SGGEQRRVALAGLLVRKPTLLVLDEPFAGLDPSGRAVLVSVLSWLVGSGLALLLISHD
                     VVEGRSVCGRVLGLSAGRVSFDGPMGGADLDSLMATAPAGPAGT"
BASE COUNT          172 a          600 c          653 g          234 t
ORIGIN      
        1 tggccggtcg agcctgaagg agcgcccggg ccggtgccgg cacgcgtcat cgatgtcggc
       61 taccgctacc gcgaggggcg cgacctcgcc ctcgacggcg tgtcgatgga ggtggaggtc
      121 ggtcagtacg tggccatcgt cgggcccaac ggctcaggca agtcgaccct gggccgggtc
      181 gtcgccggcc tgccgccgac cgcgggggcc gtcgcccgcc cgggaccggc agggctcggc
      241 cggctcggcg gcacggccat ggtcttccag cggcccgaga gccaggtgct gggggcgcgg
      301 gtcaaagacg acatccggtg gggcttgccc gctggcgcgg gcatggacac gggcacggcg
      361 ctcgaggcgg tgggcctggc cggtctggcc gatcgggaga ccgcgacgct ctccggcggc
      421 gagctccagc gggtcgccct ggccgccgcc ctggcccgcc ggcccgccct cctggtggcg
      481 gacgaggcca ctgccatgct cgacgccgac ggccgccgcg acgtggtcgc gacactccac
      541 tcgttggtgg gccaagggct gaccgtcatc gccatcaccc accacctcga cgaggtcggt
      601 cccgaggatg tcctggtccg gctcgacgcc gggagggtgg tctcgggggc ggtcaccggc
      661 gcgggttccg ggggggccaa cgggcgccct gctggctcgg ccactggctc ggccactggg
      721 gatgcccgcg gggcgggcga ggcgaccggg cccggcgcca ccccggatgc caggcccggg
      781 tcgccggagg gccggtcatt gcgacttcgc ggggtgggct acgtccacgc gtggcgttcg
      841 ccgtgggccc accgggccct ggagggcatc gacctcgacg tcgctcccgg tgaggcggtg
      901 ctcgtcgtcg gccccaacgg ggcagggaag acgacgctcg gctgggtcgt ggcgggcctg
      961 gtgcccccga cggaggggac ggtgaggttc gggccaggtc cgccgtcacc cggcgacgtc
     1021 ggcatcggct tccagcacgc ccggctccag ctcttccgcc cgacggtcga ggaggacgtc
     1081 gggctgggcg gagcggctcc ggccgacgtc gacacggcat tgcggtccgt cgccctcgac
     1141 ccggacgcgt accgtcaccg gctgatcgac gccctcagcg gcggcgaaca gcgccgggtc
     1201 gccctcgccg gcctgctggt ccggaagccc acgctgctgg tgctcgacga gcccttcgcc
     1261 gggctcgacc cctcgggtcg ggccgtcctg gtgagtgtgc tctcatggct ggtcggctcc
     1321 ggcctggcgt tgctgttgat ctcacatgac gtcgtagagg gccggtccgt gtgcggacgg
     1381 gtgctcggcc tgtcggccgg ccgggtgagc ttcgacggcc cgatgggcgg ggccgacctc
     1441 gactcgctga tggccaccgc gccggccgga ccggcgggga catgagccgg gccagcgtgc
     1501 gcagaccggt cgagctgcac ctgttgcgct tcctgcccgg ccactccccg gtgcatcgca
     1561 tgtggccggg cacgaagctc ctggtcgcgg ccgcgctcgg cctgtccgtc gggatctttc
     1621 cgacctggcc ggccgcggcc gcggtggcgg tcctgctcg
//
LOCUS       DASWGG010000263         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3139681, whole genome shotgun sequence.
ACCESSION   DASWGG010000263 DASWGG010000000
VERSION     DASWGG010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3139681"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VF954_04690"
     CDS             <1..375
                     /locus_tag="VF954_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX9530413.1"
                     /translation="VRAVLEGIAHRGADLVEAAETDSGHRIGSLRVDGGMSANGVFLQ
                     ALADAAARPVEVSPVLEATTLGAAYLAGMAVGVWGSEDEVADAWKPRAVVEPARVSDR
                     GRWLQARAKAEQTVPELSGLDF"
     gene            complement(473..>2531)
                     /locus_tag="VF954_04695"
     CDS             complement(473..>2531)
                     /locus_tag="VF954_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530414.1"
                     /translation="EHEGHLYVVRRTISGSAFTVRAQAFADGAHIAEGVRDTSRYVHS
                     ILGMDDAAFRASVFAEQKQIAAFSENAPADRRKLVLQLLGVTPLDGARDAARRDARAA
                     RDQVDRLRGLLPDAMEIEDAVADAQSRAAAADAGAAAARERADAARSELALAVERYEA
                     LDQLGRRHERLVQEGKACRDERDALAGRLDALRTELEELAGAAGRLERLRPLAATSAA
                     AEARLRAIEGVLAAMAARDEIPMVPEPAPLDEAALSELRQASTEAAAALAAINGELAG
                     ARGELDRARIAAERSAALSVEGDCPLCGQALGESFAQVQAHRALELDESQVRTRSIEG
                     RGSAAAKSAERARRAADDAERAARRAQAELAEWQLYAERRATAEAAVETALESFGAAV
                     PDEQDRQRAVAALSEARRAGSDAARLEGRLTRQPVAEAELAVGQARVDALDHDLEELR
                     GALKRLSFSPTELAGAKTALGAARSTAEDADRLQARSSGDLAAAIATLAGEGKRLEDA
                     RRQHDRLAGAEDEARHLGRAADLLNAFRNSVVATVGPRLSVQAAELFGELTDHEYDRL
                     EVDADTYEIQIRDGGRLYGMDRFSGSETDLANLALRVAISEHVRFQSGGAVGLLVLDE
                     VFGPLDEDRRERMLLAPERLRGRFRQVLVVTHASEIKDQLPAAVEVVKRPGRRATARL
                     VGQ"
BASE COUNT          285 a          981 c          946 g          319 t
ORIGIN      
        1 gtgcgcgccg tgctcgaggg aatcgcccac cgcggggcgg atctggtgga agcggccgag
       61 acggacagcg ggcaccgcat cgggtccttg cgggtcgacg gcggcatgag cgccaacggc
      121 gtgttcctcc aggccctggc cgacgccgcg gcccggcccg tcgaggtctc tcccgtcctc
      181 gaggccacca cgttgggggc cgcctacctg gccggcatgg ctgtcggggt gtggggatcc
      241 gaggacgagg tggccgacgc ctggaagcct cgcgccgtcg tcgagccggc ccgggtctcg
      301 gaccggggcc gctggctcca ggcccgggcc aaggccgagc agacggtgcc cgagctgtcg
      361 gggttggact tctagggaac cagccacccg ggcctgccgg ccgggccgag cgactggccg
      421 ccgcgctgcc ggccggccgc ggccaatggt gacgcggcca ctgccggccg ggctactggc
      481 cgacgaggcg ggcggtcgcc cggcgccctg gccgcttgac gacctcgacg gccgcgggga
      541 gctgatcttt gatctcgctg gcgtgggtca cgaccagcac ctggcggaac cggccccgca
      601 gccgctccgg ggcgaggagc atgcgctcgc gccggtcctc gtcgagaggg ccgaacacct
      661 cgtccagcac cagcaggccc accgccccgc ccgactggaa ccgaacgtgc tcgctgatcg
      721 ccacccggag ggcgaggttg gccaggtcgg tctcggaccc cgagaaccgg tccatcccgt
      781 acagccgccc cccatcgcgg atctggatct cgtaggtgtc ggcatcgacc tcgaggcggt
      841 cgtactcgtg gtcggtgagc tcgccgaaca gctcggccgc ctggaccgac agccggggcc
      901 cgacggtggc gaccacggag ttgcgaaagg cgttgagcag gtcggccgcc cgcccgaggt
      961 gacgggcctc gtcctcggcg ccggccagcc ggtcgtgctg ccggcgggcg tcctccaggc
     1021 gcttcccctc gccggcgagg gtggcgatgg cggccgccag gtcgccactg gaccgggcct
     1081 gcagccggtc ggcgtcctcg gcggtcgaac gggccgcccc gagcgcggtc ttggcgcccg
     1141 ccagctcggt cggcgagaag ctcagccgct tcaacgcgcc gcgcagctcc tcgagatcgt
     1201 ggtcgagcgc gtccacccgg gcctggccca cggccagctc ggcctcggcg acgggctggc
     1261 gggtgagccg gccctcgaga cgcgccgcgt cggatccggc ccgccgcgcc tccgacagcg
     1321 cggccacagc ccgctgccgg tcctgctcgt cgggcaccgc cgctccgaag ctctcgagcg
     1381 cggtctccac cgccgcctcg gcggtcgccc ggcgctcggc gtagagctgc cactcggcca
     1441 gctcggcctg ggctcgccgg gccgcccgct cggcatcgtc agccgcccgc cgggcccgct
     1501 cggcgctctt ggccgccgcg ctgccgcggc cttcgatcga ccgggtgcgg acctgggact
     1561 cgtccagctc gagggcgcgg tgggcctgca cctgggcgaa gctctcgccc agggcctgcc
     1621 cgcacagagg gcagtcgccc tcaaccgaca atgcggccga cctctcggcc gcgatccggg
     1681 cccgatcgag ctcgccgcgg gcaccggcga gctcgccgtt gatggcggcg agggcggcag
     1741 cagcctccgt gctcgcctgg cgcagctcgg agagggccgc ctcgtcgagg ggggcaggct
     1801 cgggcaccat cgggatctcg tcccgggccg ccatcgcggc cagcacgccc tcgatggccc
     1861 gaaggcgggc ctcggccgcg gcggaggtgg cggcgagcgg ccggagccgc tccaggcggc
     1921 cggcggcgcc cgccaactcc tccagctcgg tccggagggc gtccagccgg cccgccaggg
     1981 cgtcgcgctc gtcccggcac gcctttccct cttggaccag gcgttcgtgc cggcgcccga
     2041 gctggtcgag cgcctcgtac cgctcgaccg cgagcgcgag ctccgagcgc gccgcgtcgg
     2101 cccgctcccg cgccgcggcc gcgcccgcgt cggccgccgc cgcccggctc tgcgcgtcgg
     2161 ccacggcgtc ctcgatctcc atcgcgtcgg gcagcaagcc gcggaggcga tccacctggt
     2221 cccgggccgc cctggcgtcc ctgcgagccg cgtccctcgc cccgtccagc ggcgtgacac
     2281 cgagcagctg gagcacgagc ttgcgcctat cggcgggcgc gttctcggag aaggcggcga
     2341 tctgcttctg ctcggcgaac accgacgccc ggaaggcggc gtcgtccatt ccgaggatcg
     2401 aatggacgta gcggctggtg tcccgcacgc cctcagcgat gtgggcgccg tcggcgaagg
     2461 cctgggccct gacggtgaag gcggagcccg agatggtccg ccgcaccacg tacaggtggc
     2521 cctcgtgctc g
//
LOCUS       DASWGG010000264         3595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1854912, whole genome shotgun sequence.
ACCESSION   DASWGG010000264 DASWGG010000000
VERSION     DASWGG010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854912"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VF954_04700"
     CDS             complement(<1..1033)
                     /locus_tag="VF954_04700"
                     /inference="COORDINATES: protein motif:HMM:NF025744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4417 domain-containing protein"
                     /protein_id="HEX9530415.1"
                     /translation="MGPRLPIIAPAAGCDCRTCAFYTGNPTASEPICSGCNTDCAYCG
                     CARAGGGSKCARCPIRCGSRVDIGAWMADAGGTLAFDDVVLGLELPAQLPAFVPQVDG
                     GELAELDVGLEWPAYALGLRRVISPKTLAVYPRFAGRSAAEGMGLRPGQLAVLVGYGL
                     DPLVEAVWTRKASLLPELARQRWDLVLAPNYSMYGSQPRAEHLLNFRRNLLIAAELED
                     LGLPAVPNVYWFRKEDLDRYLAWMADVAPAAIAVNLQTFRTEADWEQMALPGLTYLST
                     GMPEATRLVVTGTSRADRLAELGALFGGRLILVSQNPLQYARHGAVMTPGGRVERRAR
                     VEDLFAANVW"
     gene            complement(1033..2211)
                     /locus_tag="VF954_04705"
     CDS             complement(1033..2211)
                     /locus_tag="VF954_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX9530416.1"
                     /translation="MSTPASVGELSSPPVQTPGSALDAARRLSASPCLVRRRLELLPL
                     SGPGSVFSDVQGRRPGASTTPEDLAELVSSIASVGVLQPILVERLPDETLRLVAGERR
                     LRAARWGAANLPPNPYFESIPAVVCPGPLPEEERRTWQIAENLVRADLAPGELAAALV
                     FERCAVLTTKLLAAGVPLPRSTVEIEDPLQRFRDLERIRLEAHLHKIGAPWEEVLGRL
                     GIQLGADSARQLYRAFSSLPPEVSTDMDAAGIGLATRLDYLRVDRGCRGAARELWEAV
                     KARGRPELFSGALRQRLDHPGLDATAALGAAEAVRDDANRARARALRPEPTGEVADPA
                     TVSAALEVMRSLVAQLRRGVRLSRYDEGTIRLCAEEILAQLPGDDVSGEGFADEGAVA
                     "
     gene            complement(2434..3270)
                     /locus_tag="VF954_04710"
     CDS             complement(2434..3270)
                     /locus_tag="VF954_04710"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEX9530417.1"
                     /translation="MTPWQTVALRRMNLDRDPWILLDLAEAGVLSTSQTRLLHFGTVE
                     ACRRRMRVLRALRLVTGFRPGGSRGAETMWALTDRGATLLGELLGETVRAGAWGRGGA
                     SARALPHRRAVTEFFMALVGKGSGPRLTPIRAAGHHAIRAAAPGEVSTLWLGERGCRT
                     VFGDPARRPLGVRAALVPDGAALLSVGEGFSPIALEMDRGTESLHVLGEKLARYAAVG
                     GLTVVVCFTDPARALWRPPDLGVDVLVGDLGSHLAEPWGPVWTGRDGRHLSLGDALEP
                     VG"
BASE COUNT          552 a         1292 c         1327 g          424 t
ORIGIN      
        1 gccacacgtt ggcggcgaag aggtcctcga cgcgcgcccg gcgctccacc cggccgcccg
       61 gagtcatcac cgcaccgtgg cgggcgtatt gcagcgggtt ctggctcacg aggatgaggc
      121 ggccaccgaa cagggccccc agctcggcca ggcggtcggc cctcgaggtc ccggtcacca
      181 caagcctcgt cgcttccggc atgcccgtcg acaggtaggt gaggcccggc agtgccatct
      241 gctcccagtc cgcctcggtg cggaaggtct ggaggttcac ggcgatcgcc gccggggcca
      301 cgtcggccat ccaggccagg tagcggtcga ggtcctcctt gcggaaccag tagacgttgg
      361 gcaccgccgg cagacccagg tcctcgagct cggcggcgat gaggaggttg cgccggaagt
      421 tgaggaggtg ctcggcgcgg ggctggcttc cgtacatcga gtagttgggc gccagcacca
      481 ggtcccagcg ctggcgggcg agctcgggga gcagcgaggc cttgcgtgtc cagaccgcct
      541 cgacgagggg atccaggccg tagccgacga gcaccgctag ctggcctggc cgcagcccca
      601 tcccctccgc cgcggagcgc ccggcgaagc gcgggtagac cgccaaggtc ttggggctga
      661 tcacccgtcg cagccccagg gcgtacgccg gccactcgag gccgacatcg agctcggcca
      721 gctctccccc gtcgacctgc gggacgaacg ccggcagctg cgcgggcagc tcgaggccca
      781 gcacgacgtc gtcgaaggca agcgtcccgc cggcgtcggc catccaggcc ccgatgtcga
      841 cccggctccc gcatcggatg gggcaccggg cgcacttgga gccgccgccc gcccgggcgc
      901 acccgcaata ggcgcaatcg gtgttgcagc cagaacagat gggctccgac gccgtcgggt
      961 tgcccgtgta gaacgcgcac gtgcggcagt cgcagccggc tgccggggcg atgatcggaa
     1021 gtcgaggccc catcaggcca ccgccccctc gtccgcgaag ccttcccccg acacgtcgtc
     1081 gccggggagc tgggcgagga tctcctcggc gcagaggcgg atggtcccct cgtcgtagcg
     1141 ggacaggcga accccgcgcc gcagctgggc cacaagggag cgcatcacct ccagggccgc
     1201 cgacacggtg gcgggatccg ccacctcgcc ggttggctcc ggacgcagcg cccgggcacg
     1261 agcccggttc gcgtcgtccc ggaccgcctc ggcggcgccg agcgcggctg tggcgtccag
     1321 gcccgggtga tcgaggcgct ggcgcagcgc tcctgagaag agctctggcc ggccgcgagc
     1381 cttgacggcc tcccagagct cccgggcggc gccccggcag ccccggtcaa cgcggagata
     1441 gtcgagacgg gtggcgaggc cgatcccggc ggcgtccatg tcggtggaca cctcgggggg
     1501 cagcgaggag aaggcgcggt agagctggcg ggcggaatcg gcacccagct ggatcccgag
     1561 ccggccgagc acctcctccc agggggcgcc gatcttgtgc aggtgcgcct cgaggcggat
     1621 ccgctcgaga tcccggaagc gctgcagcgg gtcctcgatc tccaccgtcg atctcggcag
     1681 cgggacgccg gcggcgagga gcttggtggt gagcaccgcg cagcgctcga agaccagcgc
     1741 cgcggcgagc tcgcccgggg cgaggtccgc cctgaccagg ttctccgcaa tctgccacgt
     1801 gcgccgttcc tcctcgggaa gcggtcccgg gcacaccacg gcggggatcg actcgaagta
     1861 cggattgggc gggaggttgg ccgcccccca cctcgccgcc cggaggcgac gctcccccgc
     1921 cacgaggcgg agcgtctcgt cggggaggcg ctcgaccagg atcggctgca gcaccccgac
     1981 cgaggcgatg gagctgacga gctcggccag gtcctccggc gtcgtcgagg ccccgggccg
     2041 gcggccctgc acgtcgctga agaccgaccc gggtcccgac agcggcagca gctcgaggcg
     2101 gcgtcggacg aggcacggcg aagccgacag gcgccgggcc gcgtccaggg cagaccccgg
     2161 ggtctgcacg ggaggggagg agagctctcc cacgctcgcc ggggtgctca cggcgcccac
     2221 cggatcggct ccagggagtc gcccgggcag aggccgttgc cgtgccaggg aatcacgcga
     2281 cacccccggc ccggacctgt gcacccattc acatgcacgt gttcacgtgt ccgggtgcac
     2341 gtgtacgggt tgccaaagga acgctgatgg caccacgacc tatagggcct ggtccgtaac
     2401 gcgaggagaa gtctccctcc ctcgtcgacg tagtcatcca accggctcca gggcgtcgcc
     2461 cagggaaagg tgacggccgt cccggccggt ccacaccgga ccccacggct ccgccaggtg
     2521 cgagcccagg tcgcccacca gcacgtccac gcccaggtcg ggcggccgcc agagggcccg
     2581 cgcggggtcg gtgaagcaca ctacgaccgt aaggccgccc accgcggcgt agcgggcgag
     2641 cttctcgccg aggacgtgca gggactcggt cccgcggtcc atctcgaggg cgatcggcga
     2701 aaagccctcc cccaccgaga gcagcgccgc gccgtcgggg accagggcgg ccctgacgcc
     2761 gaggggacgc cgtgccgggt cgccgaagac cgtccggcat ccgcgctcgc ccagccacag
     2821 ggtcgagacc tcgccggggg cggctgcacg gatggcgtgg tggccggcgg cccggatggg
     2881 ggtgaggcgt gggccgctgc ccttccccac cagggccatg aagaactccg tgacggcccg
     2941 gcggtgggga agcgcccgtg cggacgcgcc gccgcggccc catgccccgg ccctgaccgt
     3001 ctcgccgagc agctcgccga gcagcgtggc cccacggtcg gtgagcgccc acatcgtctc
     3061 ggcgccgcga gagccgcccg gacggaagcc cgtgacaagc cggagagccc gaaggacgcg
     3121 catccggcgg cggcaggcct cgacggttcc gaagtgcagc aggcgggtct ggctggtcga
     3181 gagaacgccg gcctcggcaa gatccaagag gatccacggg tcccggtcga gattcatccg
     3241 gcgcagcgcc acggtctgcc agggggtcac ctgggggcct ctggggagca cgtgggcggg
     3301 gcaccgcagg ggcagagcag gagggccgga ggcgatgccg gaagcgaagg aggccgggac
     3361 cgagcggagc gagggacaga agacgagccg aaaatgggtc cgctagccga agctgaacta
     3421 caaacaacac ctagcggacg tattttcgcc cttttgcccg cccggccaag gatcacaagg
     3481 ggcggccctc cacgatgttc gtcttcccgg ccgagatctg cgcctcctgt ccgctgcggg
     3541 agtgctgcac caaggccaag cacgccagcc ggacggttgc cgtcggcgtg cacga
//
LOCUS       DASWGG010000265         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_436450, whole genome shotgun sequence.
ACCESSION   DASWGG010000265 DASWGG010000000
VERSION     DASWGG010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436450"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /gene="thrS"
                     /locus_tag="VF954_04715"
     CDS             <1..1417
                     /gene="thrS"
                     /locus_tag="VF954_04715"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX9530418.1"
                     /translation="SAYRNTESFVDLCRGPHVPTTARLGSFKLTRVAGAYWRGDEKRP
                     QLQRIYGTAWESDKALAEHLHRLEEAEKRDHRRLGAELDLFSFPDEIGSGLAVFHPKG
                     GLVRRLMEDYSRQRHEAAGYAFVNSPHIAKSTLFETSGHLQWFADSMYPPMELEGADY
                     YAKPMNCPFHMLIFRARGRSYRELPLRMFEFGAVYRYEKSGVVHGLTRVRGMTQDDAH
                     IFCTPEQMPEELSSLLGFVLSVLRDYGLKDFYLELSTKPEGKAIGTDEEWASATEALR
                     QAAEREALELVMDEGGGAFYGPKISVQAKDAIGRSWQLSTIQLDFQEPQRFGLEYAGA
                     DNERHQPVMIHRALFGSVERFFAVLLEHYAGAFPAWLSPVQVAVLPVRDDHGAYATSV
                     SERLRAGGFRVEVSAADEPLGARIRKAKLERIPYILVAGDDDVAAGTVGVNDRVSARP
                     ERDVPVEVFLDRLRVEVETRA"
     gene            1414..1932
                     /locus_tag="VF954_04720"
     CDS             1414..1932
                     /locus_tag="VF954_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX9530419.1"
                     /translation="MSLERLWAGWRSGYVSGLTGGSADAPSGGATGCVFCRILSSGEP
                     DSATYVVWRGGCTAAVLNAFPYTSGHVLVLPLRHVAEPGELAADEAAELWASVTAAVA
                     ALRGAYRPQGINLGANLGEAAGAGVPGHFHVHALPRWNGDTSFMTSLAEVRVLPEALP
                     DTWERLRSAWPS"
     gene            1956..>2350
                     /locus_tag="VF954_04725"
     CDS             1956..>2350
                     /locus_tag="VF954_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530420.1"
                     /translation="MKDEPVEDEQGDELPAELDVTGLVGPYTFPDNSRRRIAAALYLG
                     AAVLCVWLWAARHHNGVLVNGGFLAVGVALAAVGLYQWAAGYRLATTETDALVAASRQ
                     VGFPVGHASAQLGWRGLRSRPTWRILLYSA"
BASE COUNT          318 a          831 c          862 g          339 t
ORIGIN      
        1 cagcgcatac cgcaacacgg agagcttcgt cgatctgtgc cgcgggccgc acgtcccgac
       61 caccgcccgg ctgggctcgt tcaagctcac ccgggtggcg ggcgcgtact ggcgggggga
      121 cgagaagcgc ccccagctcc agcgcatcta cggcacggcg tgggagtcgg acaaggcgct
      181 cgccgagcac ctccaccggc tcgaggaggc cgagaagcgg gaccaccggc ggctcggcgc
      241 ggagctcgac ctcttctcct tccccgacga gatcggcagc gggctggccg tgttccaccc
      301 caaggggggc ctggtccgca ggctgatgga ggactactcc aggcagcgcc acgaggcggc
      361 cggctacgcc ttcgtcaaca gcccccacat cgccaagtcg acgctgttcg agacgtcggg
      421 gcacctgcaa tggttcgccg actccatgta cccgcccatg gagctggaag gagcggacta
      481 ctacgccaag cccatgaact gcccgttcca catgctcatc ttccgggcgc ggggccgctc
      541 gtaccgggag ctgccgctgc ggatgttcga gttcggcgcc gtctaccgct acgagaagtc
      601 gggggtggtg cacggcctca cccgggtgcg gggcatgacg caggacgacg cccacatctt
      661 ctgcaccccc gagcagatgc ccgaggagct gtcgtcgttg ctcggcttcg tgctgtcggt
      721 cctgcgcgac tacggcctga aggacttcta cctcgagctg tccaccaagc ccgagggcaa
      781 ggccatcggc accgacgaag agtgggcctc ggccaccgag gccctccgcc aggcggccga
      841 gcgggaggcc ctggagctgg tgatggacga gggcggcggg gccttctacg ggcccaagat
      901 ctcggtgcag gccaaggacg caatcggtcg cagctggcag ctgtcgacca tccagctcga
      961 cttccaggag ccccagcgct tcggcctgga gtacgccggc gctgacaacg agcggcacca
     1021 gccggtgatg atccaccgcg ccctgttcgg ctcggtggag cgcttcttcg ccgtgctgct
     1081 cgagcattac gcaggggcct tcccggcgtg gctgtctccc gtgcaggtgg cggtcctgcc
     1141 cgttcgcgac gaccacggcg cttacgccac gtcggtgagc gagcgcctcc gggccggcgg
     1201 cttccgggtc gaggtgagcg cggccgacga gccgctcggc gcgcgcatcc gcaaggccaa
     1261 gctggagcgg atcccgtaca tcctggtggc gggcgacgac gacgtggccg cggggaccgt
     1321 gggggtcaac gatcgggtgt cggcgcggcc ggagcgtgac gtgcccgtcg aggtgttcct
     1381 ggaccggctc cgcgtcgagg tggagaccag ggcgtgagcc ttgagcggct ctgggcgggc
     1441 tggcgcagcg gctacgtcag cgggctcacc ggcgggtccg ctgacgcccc gagcggaggg
     1501 gcgaccggct gcgtcttctg ccggatcctc tcgagcgggg agcccgactc ggccacctat
     1561 gtggtgtggc ggggagggtg cacggccgcg gtgctcaacg ccttccccta caccagcggg
     1621 cacgtgctcg tgctgcccct gcgccacgtg gccgagcccg gggagctcgc cgccgacgag
     1681 gccgccgagc tgtgggcgtc ggtgaccgcc gcggtcgccg ccctgcgggg cgcctaccgg
     1741 ccccagggga tcaacctcgg ggccaacctg ggggaggcgg ccggcgccgg cgtgcccggg
     1801 cacttccacg tccacgccct gccccggtgg aacggggaca ccagcttcat gacctccctc
     1861 gccgaggtcc gggtcctgcc cgaggcgctg cccgacacct gggagaggct ccggtcggct
     1921 tggccgtcct gagcccgcca tcttacggtg atggggtgaa ggacgaaccc gtggaggacg
     1981 aacagggcga cgagctcccg gccgagctcg acgtcaccgg cctggtcggg ccctacacct
     2041 tccccgacaa cagccgccgg cgcatcgcgg cggcgctgta cctgggcgcc gccgtcctgt
     2101 gcgtgtggct gtgggccgcc cgccatcaca acggcgtcct ggtcaacggc ggcttcctgg
     2161 ccgtgggcgt ggccttggcg gcggtcgggt tgtaccagtg ggcggccggg taccgcctgg
     2221 cgaccaccga gaccgacgcg ctggtggcgg ccagccgcca ggtcggcttt cccgtgggac
     2281 acgcctcggc gcagctgggc tggcgggggc tgcggagccg gccgacgtgg cgcatccttc
     2341 tctactcggc
//
LOCUS       DASWGG010000266         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2369542, whole genome shotgun sequence.
ACCESSION   DASWGG010000266 DASWGG010000000
VERSION     DASWGG010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2369542"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /locus_tag="VF954_04730"
     CDS             complement(<1..1560)
                     /locus_tag="VF954_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX9530421.1"
                     /translation="MSLVEGRADLRSDPRLWAALMAGGLVYWAWNGIAVTAAIALSQG
                     LNWWTVYRKGLLLSLLVAAGNTTTGVLLVVIAKTNPVALVGVPMLCGVLLLAYRGYIK
                     AMRERDTWQVLQEVSNDLLQLDEEALAHVVIDGARRLFGAEFAELVIAPGGPTDAVIA
                     FGQVAGKPMSRDHGSPFVVAAPFWGRVLADREPFQVSGRRAAAPQREELTRLGLATCA
                     VIPLISGRGCIGCLRIGFQGDVSLGGRELQVFATYGSHVTAAVSNAQLFEEMRNLALH
                     DVLTGLPNRSLLQDRLLQALERSRRTRTHTAVLFVDLDRFKVINDSLGHETGDQVLQA
                     VAGRLVDHLRPGDTATRFGGDEFVVVCEGVVDAAEAQRLGDRLVEIFETPFVLPTGRE
                     AFVSASVGVVLAEAGMDDPGALLGHADAAVYRAKERGRACCALFDEALSTRVVTRLER
                     EEELRHAIGANELHLHYQPLVRLSDQRIVGSEALLRWRHPRLGALAPAEFIPLAEETG
                     LIVTVGTWALRL"
BASE COUNT          242 a          574 c          588 g          213 t
ORIGIN      
        1 caggcgcagc gcccaggtcc cgacggtcac gatgaggccc gtctcctcgg cgaggggtat
       61 gaactcggcc ggcgccagcg cgccgagcct ggggtgccgc cacctcagga gggcctcgga
      121 gccgacgatg cgctgatcgg acagccgcac gaggggctgg tagtgcaggt gcagctcgtt
      181 ggcgccgatg gcgtggcgca gctcctcctc tcgttcgagg cgggtgacga cgcgggtcga
      241 cagcgcctcg tcgaacagcg cgcagcaggc ccgcccgcgc tccttggccc ggtagacggc
      301 cgcgtctgcg tggcccagca gggccccggg gtcgtccatc cccgcctcgg cgaggaccac
      361 gcccacgctg gcgctgacga acgcctccct ccccgtcggg agcacgaagg gcgtctcgaa
      421 gatctccacc agacggtcgc ccaggcgctg ggcctcggcc gcgtcgacca cgccctcgca
      481 cacgaccacg aactcgtcgc cgccgaaacg ggtggcggtg tcgcccgggc gcaggtggtc
      541 gacgagccgg ccggcgaccg cctgaaggac ctggtcgccc gtctcgtgtc cgagcgagtc
      601 gttgatgacc ttgaagcgat cgaggtcgac gaacagcacg gcggtgtggg tcctcgtgcg
      661 ccgcgaccgc tccagcgcct ggaggaggcg gtcctggagc agcgagcggt tcggcagccc
      721 ggtgaggacg tcgtggaggg cgaggttgcg catctcctcg aagagctggg cgttgctcac
      781 ggccgcggtg acatggctgc cgtaggtcgc gaacacctgg agctcccggc ctccgaggct
      841 gacgtcgccc tggaagccga tgcgcaggca tccgatgcaa ccccggccac tgatgagggg
      901 gatgacggcg caggtcgcca gcccgagccg ggtcagctcc tcgcgctggg gcgcggccgc
      961 tctacggccc gagacctgga agggctcgcg gtcggcgagc acccgacccc agaacggggc
     1021 cgcgaccacg aacggggacc cgtggtcgcg gctcatgggc ttgccggcga cctgaccgaa
     1081 ggcgatcacc gcgtcggtcg gcccgcccgg tgcgatcacg agctcggcga actcggcgcc
     1141 gaacagccgg cgggcgccgt cgatgaccac gtgggccagg gcctcctcgt cgagctggag
     1201 caggtcgttg gagacctcct ggagcacctg ccaggtgtcc cgctctctca tggccttgat
     1261 gtagccccgg taggcgagga gcaggacgcc gcacagcatc gggaccccga ccagcgccac
     1321 cgggttggtc ttggcgatga cgacgaggag gacgcccgtg gtggtgttgc ccgcggcaac
     1381 cagcaggctg agcaacaggc ccttgcggta caccgtccac cagttgaggc cctgcgacag
     1441 ggcgatggcg gccgtgacgg cgatcccgtt ccaagcccag tacaccagtc cgcccgccat
     1501 gagcgccgcc cacagcctcg ggtcgctgcg gaggtcggct cggccttcga cgaggctcat
     1561 caccgtgcgg gccaggacga ccccggtagc cgccacggag gcgttgaacc ccacctt
//
LOCUS       DASWGG010000267         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1856226, whole genome shotgun sequence.
ACCESSION   DASWGG010000267 DASWGG010000000
VERSION     DASWGG010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856226"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VF954_04735"
     CDS             complement(<1..327)
                     /locus_tag="VF954_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9530422.1"
                     /translation="MAGTGTEVGKTWVASGLARRLQSAGLTVAARKPAQSDSPGTDEE
                     ETDAGRLAAATGATPGEVCPRHRWYPLAMAPPMAADALGREPVRVADLAAETTWPPSV
                     EVGLVET"
     gene            complement(341..1492)
                     /locus_tag="VF954_04740"
     CDS             complement(341..1492)
                     /locus_tag="VF954_04740"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX9530423.1"
                     /translation="MTSWATWAGTELSAIRAAGRWRVVRDLDGRGQVHSLAGVEGAVV
                     SFASNDYLGLTTHPTVVSAAHEALDRWGAGSGSARLIVGSRPVHTALETELAGWKETE
                     AALLFPTGFAANLGVLSALGTADVTIVSDELNHASIIDGCRLARSEVAIARHGDLDHF
                     AALVAGARARGRRPLVVTETVFSMDGDRAPLAGLASLCADHDALLVLDEAHSVLGPAP
                     AQSAGGGLLRVGTLSKTLGALGGFAAGPRALIDLLRNRARPFIFTTASTPADTAAALA
                     ALHVLRGPEGAALVTRLRGLVARLRPGHGSPIIPVVLGGEADALAAADALLGRGMLVP
                     AIRPPTVPVGTSRLRIALSAAHTDHQLDQLSLALGELGLHDRGAVGVAT"
     gene            complement(1489..>2306)
                     /locus_tag="VF954_04745"
     CDS             complement(1489..>2306)
                     /locus_tag="VF954_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9530424.1"
                     /translation="AGGFGTDVFGPLRFPVLRAPDFDDPRCFDQAVAMVAAHASSLAA
                     VVIEPLVQGAAGIMLAAPADVSRLGEACRTHGVLLVADEVATGFGRTGTLFASEQCGI
                     RPDLLCLGKGLTGGYLPMSATVASQRVFDGFEGPDLSDRTLYHGHSYSGNALAAAVAL
                     RHLQLVTEGAVLKNVRARAAQLEGLLRREIGGHGGVRSVRQRGLMIGVELAPPAEGLR
                     WGRQVCAAAVRRGVLLRPIGDVVVVMPPLTVTASEVERIVTTLAEAIAEVCGG"
BASE COUNT          316 a          847 c          892 g          251 t
ORIGIN      
        1 cgtctcgact aggcccacct cgaccgaggg cggccaggtg gtctccgccg ccagatcggc
       61 gacccggact ggttcccgcc cgagagcgtc ggcggccatc ggaggcgcca tggcgagcgg
      121 gtaccagcgg tggcgggggc agacctctcc aggggtggcg ccggtggcgg ccgccaggcg
      181 gccggcgtcg gtctcctcct cgtcggtgcc aggggagtcg gactgggccg gcttgcgcgc
      241 cgccactgtg agccccgcgc tttgcagccg gcgggccagt ccggatgcca cccaggtctt
      301 gcccacctcc gtgcccgtgc cggccacgag gacgagaagc tcaggtcgcg acgccgacgg
      361 ctccgcggtc gtgcagcccg agctccccga gcgcgagcga aagctggtcg agttggtggt
      421 ccgtgtgggc cgccgagagg gcgatgcgca gccggctggt cccgaccggg accgtgggcg
      481 gccggatcgc cggcacgagc atcccgcgcc cgaggagggc gtcggcggca gccagggcgt
      541 cggcctcgcc gccgagcacc acggggatga tcggcgaacc atgcccaggg cggagccggg
      601 ccaccaggcc gcggaggcgg gtcaccagcg cggctccctc ggggccgcgc agcacgtgca
      661 gcgcggccag ggccgcggcc gtgtcggccg gcgtggaggc ggtggtgaag atgaacgggc
      721 gggcccggtt gcgcagcagg tcgatgagag cccgcggccc ggccgcgaag ccgcccagcg
      781 ccccgagggt cttggacagc gtgccgactc ggagcaggcc accgcccgcc gactgcgccg
      841 gcgccggccc gagcacggag tgggcctcgt cgagcacgag cagggcgtcg tggtcggcgc
      901 acagcgacgc caggccggcg agcggcgccc ggtctccatc catcgagaag accgtctcgg
      961 tgacgaccag gggccgccgt ccccgggccc gggcgccggc gaccagggcg gcgaagtggt
     1021 cgaggtcgcc gtggcgggcg atggccacct ccgagcgcgc cagccggcag ccgtcgatga
     1081 tcgaggcgtg gttgagctcg tcgctgacga tggtcacgtc ggctgtgccg agcgccgaca
     1141 ggacgccgag gttggccgcg aacccggtcg ggaagaggag ggccgcttcg gtctccttcc
     1201 acccggccag ctcggtctcg agggcggtgt gcaccgggcg cgacccgacg atgaggcggg
     1261 ccgaccctga ccctgcgccc caccggtcga gcgcctcgtg ggcggcggac acgacggtcg
     1321 gatgggtcgt gagcccgagg tagtcgttgg acgcgaagga gaccacggcc ccctcgacgc
     1381 cggcgaggga gtggacttgg ccccgcccgt cgaggtcgcg aaccactcgc caccggccgg
     1441 cggcccggat ggccgagagc tcggtgcccg cccacgtggc ccaggacgtc acccgccgca
     1501 cacctcggcg atggcctcgg cgagggtggt cacgatgcgc tcgacctcgg acgcggtcac
     1561 ggtcaacggc ggcatgacga ccaccacgtc gccgatcggc cgcagcagca cgccccggcg
     1621 cacggcggcc gcgcagacct ggcggcccca gcgcaggccc tcggccggtg gcgccagctc
     1681 cacgccgatc atcagccccc gctgccggac cgaacgcacc ccgccgtggc cgccgatctc
     1741 gcgccgcagc agcccctcca gctgggcggc ccgggcccgc acgttcttca gcacggcgcc
     1801 ttcggtgacc agctgcaggt gccggagggc gacagcggcg gccagggcat tgccgctgta
     1861 cgaatgcccg tggtacagcg tgcggtcgct caggtccggt ccctcgaacc cgtcgaacac
     1921 ccgctgcgag gccaccgtgg cggacatcgg caggtacccg ccggtgaggc ccttgcccag
     1981 gcacagcagg tcggggcgga tgccgcactg ctcgctggcg aagagcgtgc cggtgcggcc
     2041 gaagccggtg gccacctcgt cggcgacgag cagcacgccg tgggtgcggc acgcctcgcc
     2101 gaggcgggac acgtccgccg gcgccgccag catgatgccg gccgcgccct ggaccaaagg
     2161 ctcgatcacc acggctgcga ggctcgaggc gtgggccgcg accatggcga cggcctggtc
     2221 gaagcaccgg ggatcgtcga aatcgggagc gcgcaggacg gggaagcgca gtggcccgaa
     2281 cacatcggtg ccgaacccgc cggctc
//
LOCUS       DASWGG010000268         3689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1530390, whole genome shotgun sequence.
ACCESSION   DASWGG010000268 DASWGG010000000
VERSION     DASWGG010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530390"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VF954_04750"
     CDS             <1..321
                     /locus_tag="VF954_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEX9530425.1"
                     /translation="PEFINRIDEIVRFRQLTRDDIARIVEIQLRHLDRRLAERRLSLE
                     VTDGARAWLAEHGYDPIYGARPLKRLISRAVSDTLALALLEGRYPEGGTVVVDADESG
                     ILLR"
     gene            389..1219
                     /locus_tag="VF954_04755"
     CDS             389..1219
                     /locus_tag="VF954_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9530426.1"
                     /translation="MSAAEGARRVTVGLVDDLDDVRFLLRLAFERDERFEVVGEAADG
                     RQAVELAARCQPDLLVLDSNMPVMGGVEAIPHIRRASPRTAVLLYTAGVDRTTAQAAV
                     AAGAVDVLEKMPPGPTMVDMVAHVLVDHWAGPDAAIHVEVGPVPQAAARVWLANTSRL
                     VAAVRAHPELFAEPIPADVLDDFEGFLATWRSLAGEGEEFHWIARAKPADVRRLVTHW
                     AALDRMTDEQLAALEVTWSPPEGTPFFEALVEGVLAALSAHDETQQLAVALTRQWATA
                     "
     gene            1263..2486
                     /locus_tag="VF954_04760"
     CDS             1263..2486
                     /locus_tag="VF954_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530427.1"
                     /translation="MRALAALVALLLVAGALGAVSVDVQGGGSSPRLAAVPPPTTTAA
                     SAAPTAKTAAPATTPLATAVAPVPPALEAVIHQLMAFVEQARGLKFKQDVPVELLGAA
                     AFQARLDAGAGVSQADATKTEKVLRALGLLPAHVDVAAEERKLTTGAVIGLYDPRSRS
                     LLIRGAEPTPLVRFTLVHELTHALQDQWFGLDRPDIAKRNDESSLAFSSLIEGDAVRI
                     QNAYLRSLPAAQQDAANKEEQAVAPDLGSIPHVLVELMYFPYTYGLRFVTSVVGVAGQ
                     SRLDAAFRDPPTTSSQILHPDRFVAGDPPVPVAQPPAGGTLVDDGPLGQIGLFLVLSS
                     QLGTDTAAHATDGWAGDDYAAYDAGAATCVRDAVVMDSATDAAALDRALHAYARKRAG
                     VTIEGGDPYQWTACG"
     gene            2596..>3689
                     /locus_tag="VF954_04765"
     CDS             2596..>3689
                     /locus_tag="VF954_04765"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX9530428.1"
                     /translation="MPATVVVGTQWGDEGKGKLTDLLAKEMSMVVRYQGGHNAGHTIV
                     VDGERFALQLLPSGVLYGHITPVIGNGVVVDPGVLLDEVDALEARGVDCSRLRVSGNA
                     HLIMPYHQELDRLTERFLGKNKLGTTKRGIGPAYADKATRIGLRVQDLLDPKIFREKL
                     HLVVKEKNLVLAKVYNRLPASAEEIAARYLDEYAPRLEPMIVDAVSLVHEALEAGQHV
                     LLEGAQATFLDLDHGTYPFVTSSNPVAGGGCVGAGVGPLDISRVVGIAKSYVTRVGSG
                     PFPTEMNGSEADDLIERGHEWGTNTGRRRRVGWFDCVMMRQAVRLNSLSEIALTKLDL
                     LGTFDTVKVCVAYRHDGIRHDTLPYHQSTL"
BASE COUNT          526 a         1407 c         1262 g          494 t
ORIGIN      
        1 ccggagttca tcaaccggat cgacgagatc gtccgcttcc ggcagctcac ccgggacgac
       61 atcgcccgca tcgtggagat ccagctacgg cacctggacc gccggctggc ggagcggcgc
      121 ctgtcgctgg aggtgaccga cggggcccgg gcgtggctgg ccgagcacgg ctacgacccg
      181 atctacgggg cccggccgct gaagcggctg atcagccggg cggtcagcga caccctggcc
      241 ctggccctgc tcgaggggcg ctaccctgag ggcggcaccg tggtcgttga tgccgacgag
      301 agcgggatcc tcttgcgcta gatcgttcga caccttttcc ctcaaaaagc gggcccgggt
      361 gccgatggtc acctgggagg gagacggagt gagcgcggct gaaggcgccc ggcgggtgac
      421 ggtgggcttg gtcgacgacc tggacgacgt gcgcttcctg ctgcgcctgg ccttcgagcg
      481 cgacgagcgc ttcgaggtgg tcggcgaagc cgccgacggc cgccaggccg tcgagctggc
      541 cgcccgctgc cagcccgacc tcctggtgct ggacagcaac atgcccgtca tgggcggcgt
      601 ggaggccatc ccgcacatcc ggcgggcgtc gccgcgcacc gccgtgctcc tctacaccgc
      661 cggtgtcgac cggaccacgg cccaggccgc cgtcgccgcg ggtgcggtcg acgtgctcga
      721 gaagatgccg cccggtccca ccatggtcga catggtggcc catgtgctgg tcgaccactg
      781 ggccggtccc gacgcggcca tccacgtcga ggtcggcccc gtcccccagg ccgcggcccg
      841 ggtgtggctg gccaacacgt cccggctggt ggcggccgtg cgggcccatc cggagctgtt
      901 cgccgagccc atacccgccg acgtgctcga cgacttcgag ggcttcctgg ccacgtggcg
      961 gagcctggcc ggcgagggcg aggaattcca ctggatcgcc cgggccaagc ccgccgacgt
     1021 ccgccgcctg gtcacccact gggccgcgct cgaccgcatg accgacgagc agctggccgc
     1081 cctggaggtc acctggtcgc cgcccgaggg cacgcccttc ttcgaggccc tggtggaggg
     1141 cgtgctcgcc gccctctcgg cccacgacga gacccagcag ctggccgtgg ccctcacccg
     1201 gcagtgggcc accgcctgag ggccccggcc tgaccaccac ggtcgccccc tcccgcggcg
     1261 ccatgcgggc cctggccgcc ctggtggcgc tcctgctggt ggccggggcg ctgggcgcgg
     1321 tgtcagtcga cgtgcagggc gggggctcca gcccgcgcct ggcggccgtc ccgccgccca
     1381 ccaccactgc cgcgagcgcc gcgcccacgg ccaagacggc tgcgcctgcg accacgcccc
     1441 ttgcaaccgc ggtagccccc gtgcccccgg ccctggaagc cgtgatccac cagctcatgg
     1501 ccttcgtgga gcaggcccgg ggcctcaagt tcaagcagga cgtgcccgtc gagctgctcg
     1561 gcgccgccgc cttccaggcc cggctcgacg ccggcgccgg cgtcagccag gccgacgcca
     1621 ccaagacgga gaaggtgctg cgggccctcg ggctgctccc cgcccacgtg gacgtggccg
     1681 ccgaggagcg caagctcacc accggggcgg tgatcggcct ctacgacccc aggtccagga
     1741 gcctgctcat ccggggcgcc gagccgacgc cgctggtgcg cttcacgctc gtgcacgagc
     1801 tgacccatgc cctccaggac cagtggttcg gcctggaccg gcccgacatc gccaagcgca
     1861 acgacgagtc gtccctcgcc ttctcctcgc tgatcgaggg cgacgccgtg cgcatccaga
     1921 acgcctacct gcgctccctc ccggccgccc agcaggacgc ggccaacaag gaggagcagg
     1981 ccgtggcccc cgacctgggc tccatcccgc acgtgctcgt cgagctgatg tacttcccgt
     2041 acacctacgg gttgcgcttc gtgaccagcg tggtcggcgt cgccggccag agccggctgg
     2101 acgcggcgtt cagggacccg cccacgacca gctcccagat cctgcacccc gaccgcttcg
     2161 tggcgggcga cccgcccgtg ccggtggccc agccgcccgc gggcgggacc ctggtcgacg
     2221 acggccccct gggccagata gggctcttcc tcgttctgag cagccagctc ggcaccgaca
     2281 ccgcggccca cgccaccgac gggtgggcgg gagacgacta cgcggcctac gacgccggcg
     2341 ccgccacgtg cgtgcgcgac gcggtcgtca tggacagcgc caccgatgcc gccgcgctcg
     2401 accgcgccct gcatgcctac gcccgcaagc gggccggcgt gaccatcgag gggggcgacc
     2461 cctaccagtg gaccgcctgc gggtgaggcc gccaacctgg tgaggggcgc gacccttgcc
     2521 cggcaccggc gccccttttc ccccaacggg tatattgacc ggaccgcagc accacctttt
     2581 ggaaggaatc cgcgcgtgcc cgcgaccgtg gtcgtcggaa cccagtgggg agacgaaggc
     2641 aagggcaagc tcaccgacct cctggccaag gagatgtcga tggtggtgcg ctaccagggt
     2701 ggccacaacg ccggccacac gatcgtggtc gacggcgagc gcttcgcact ccagctgctc
     2761 cccagcggcg tgctctacgg ccacatcacg ccggtgatcg gcaacggggt ggtggtcgac
     2821 cccggggtcc tgctcgatga ggtcgacgcc ctcgaggccc ggggcgtgga ctgcagccgc
     2881 ctgcgcgtca gcggcaacgc ccatctgatc atgccctacc accaggagct ggaccggctc
     2941 acggagcgct tcctcggcaa gaacaagctg ggcaccacca agcggggcat cggcccggcc
     3001 tacgccgaca aggccacccg catcggcctg cgcgtccagg acctcctcga ccccaagatc
     3061 ttccgcgaga agctccacct ggtggtcaag gagaaaaacc tggtgctggc caaggtctac
     3121 aaccgcctgc ccgcctcggc cgaggagatc gcggcccgct atctcgacga gtacgcaccc
     3181 cgcctcgagc ccatgatcgt ggacgccgtg agcctggtgc acgaggccct ggaggcgggc
     3241 cagcacgtgc tgctcgaggg ggcccaggcc accttcctcg acctcgacca cggcacctat
     3301 ccgttcgtga cctcgtccaa cccggtggcg ggcgggggct gcgtcggcgc cggcgtcggg
     3361 cccctcgaca tctcccgggt ggtgggcatc gccaagtcct acgtcacccg ggtgggcagc
     3421 gggccgttcc cgaccgagat gaacggctcc gaggccgacg acctgatcga gcggggccac
     3481 gagtggggga ccaacacggg ccggcgccgg cgggtcgggt ggttcgactg cgtgatgatg
     3541 cgccaggccg tgcgtctcaa ctcgctctcg gagatcgccc tgaccaagct cgacctgctc
     3601 ggcaccttcg acaccgtcaa ggtctgcgtg gcctaccgcc acgacggcat ccgccacgat
     3661 accctgccgt accaccagtc gaccctgca
//
LOCUS       DASWGG010000269         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2811597, whole genome shotgun sequence.
ACCESSION   DASWGG010000269 DASWGG010000000
VERSION     DASWGG010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2811597"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VF954_04770"
     CDS             complement(<1..264)
                     /locus_tag="VF954_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9530429.1"
                     /translation="MPVAAAVYPVHLVVAGRRVLVVGGGRVAARKVAGLLPCGADVTV
                     VAPELGPELATLHQADQPDDPDRPGRLQVARRPYERGEAASYEL"
     gene            complement(282..974)
                     /locus_tag="VF954_04775"
     CDS             complement(282..974)
                     /locus_tag="VF954_04775"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX9530430.1"
                     /translation="MRRIPEATVARLPVYLRCLLELAGSATPTVSSERLAELAGVNAA
                     KLRKDLSYLGSYGTRGVGYDVEYLLYEIQRELGLTQDWPVVIVGLGNLGQALANYRGF
                     PARGFRVVALVDADPQKVGGRIGDLPIHAVDDLPRIVAEHAVAIGIIATPAPVAQDVA
                     DRLVAAGVGSILNFAPAVVTVPSHVPLRKVDLSIELQILTFYQQRHDELRRANDPSAN
                     GPQAIGQPVVSL"
     gene            1159..2106
                     /locus_tag="VF954_04780"
     CDS             1159..2106
                     /locus_tag="VF954_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530431.1"
                     /translation="MTLQIALAVAAALVALAFGMSTFERWLDRRRPHELAWSVALGLF
                     CLAASAMAAGGAAGWSGATFRAFYLFGAVLDVPFLALGTIYLLLGRRQGDRWALLIGL
                     AAAFAVGVVLQAPFRRGHTPAGAVLPQGSQVFGALPRVLAAFASAAGAGVVLAGAAWS
                     AVRFWRAAREARAAPAGRAAPAGAPPSGPPAGRMATANAMIALGTLILGASGLLNSVA
                     DAMTAFSASLLVGITVLFAGFLVASPDRAATAAGPHPGRLSPDPPGDPGAPGAATDGA
                     ATQEGPGAEPCPPPLAGGSRRTSPSSGTCSGPAATRPRR"
     gene            complement(1988..3568)
                     /locus_tag="VF954_04785"
     CDS             complement(1988..3568)
                     /locus_tag="VF954_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9530432.1"
                     /translation="MANLSTILDGHPDGAAALVSRGQVTTYGELVEQVGRLRGGLVGL
                     GVRPGERVALLAANNWYFVVSYLAALGTGAAIVPLNPTSPTAELHGELAAVRARLAIA
                     SPSAVAALSGVDRAAVGLEQVVVGAAEGASVDGAVTLESVMAGSPAPAVERADGDIAV
                     MIFTAGTAGAPKPAMLSHGALLANLEQVQRHPGRTVRADDVVLGVLPMFHIFGLNVML
                     DLALLAGASVVLVERFEPDAALETIAARGVTVVGGSPTMFAALAAVPGATGAELRSVR
                     LAVSGAAALTDEVADSFTARFGIPLWQGYGLTEAGPVVTSSVVGGEGAGGEGAGGEGA
                     GGEGAGGEGGGGDVRRSSIGVPIPGVDVRLVDEEGEDALEGDPGEIWVKGPNLFSGYW
                     NDPEATDRALTADGWLRTGDMAVADDDGYLYIVDRVKDVIIVSGFNVYPAEVEEVLLA
                     HPGIAEVAVAGRPHPYSGETVVAFVVPERGAAIEEDDVIAWAGSRLARYKCPTKVTFV
                     ESLPQGVAGKVLRRALPG"
     gene            complement(3751..>4175)
                     /locus_tag="VF954_04790"
     CDS             complement(3751..>4175)
                     /locus_tag="VF954_04790"
                     /inference="COORDINATES: protein motif:HMM:NF042701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM hydroxide adenosyltransferase"
                     /protein_id="HEX9530433.1"
                     /translation="PTFAGRDVFAPVAAHLCNGVDLASLGEELDPATLRPGLLPLPRQ
                     EDGWLLAEVLWVDRFGNAQLNVGPEEVTAFGERLALRCKDQLRTGRRCGAYAELGPGE
                     IGVLVDSYGLLALSMDQASAADQLGLRAGDEVAIGEAS"
BASE COUNT          538 a         1579 c         1536 g          522 t
ORIGIN      
        1 cagctcatag cttgccgcct cgccgcgctc ataggggcgt cgcgccacct gcagccggcc
       61 gggccggtcg ggatcgtcag gctggtcagc ctggtggagg gtcgccagct ccgggccgag
      121 ctccggcgcg accaccgtca cgtcggcgcc gcagggcagc agcccggcca ctttgcgggc
      181 cgccacccgg cccccgccga ccaccaggac gcggcgcccc gccaccacca ggtgcacggg
      241 gtacacggca gctgcgacgg gcacggctgt cctcgaggcc ctcagaggct caccacgggc
      301 tggccgatgg cctggggccc gttggcactg gggtcgttgg cccggcggag ctcgtcgtgg
      361 cgctgctggt agaaggtgag gatctggagc tcgatcgaga ggtccacctt ccgcaggggc
      421 acgtgggagg ggacggtcac caccgccggg gcgaagttga ggatggagcc gacgccggcg
      481 gccaccaggc ggtcggccac gtcctgggcc acaggggccg gggtggcgat gatgccgatg
      541 gcgaccgcgt gctccgcgac gatccggggc aggtcgtcca cggcgtggat gggcagatcg
      601 ccgatgcgcc cgccgacctt ctgcgggtcg gcgtccacga gggcgacgac ccggaagccg
      661 cgggccggga agccccggta gttggccagg gcctggccta ggttgcccag accgacgatg
      721 acgaccggcc agtcctgggt gaggcccagc tcccgctgga tctcgtacag caggtactcg
      781 acgtcgtagc cgacgccccg cgtgccgtag ctgcccaggt aggagaggtc cttgcgcagc
      841 ttggcggcgt tgactccggc cagctcggcc aggcgctcgg acgagaccgt cggcgtggcc
      901 gacccggcca gctcgagcag gcagcgcagg tacaccggga ggcgggcgac cgtggcctcc
      961 gggatgcgcc tcacccctcc ctccttcatg ccgatcacca taccgagccg cggtgggcat
     1021 tcacaatgtc actagcgggc ctgggcccgt acgggcggcg gcgccgcggt tccgagcacc
     1081 gaaagggggg gaagacggcg ccaaacggtg ccaggaatgc ccgaccggtg cgggtccggc
     1141 tctagcctgg cgaccgacat gacgttgcag atcgcgctgg cggtggccgc cgccctcgtc
     1201 gcgctggcct tcgggatgtc gaccttcgag cgctggctgg accggcggcg cccccacgag
     1261 ctggcgtggt cggtggcgct ggggctgttc tgcctggcgg cctcggccat ggcggccggc
     1321 ggcgcggccg gatggtcggg tgcgacgttc cgggctttct acctcttcgg cgcggtcctg
     1381 gacgtcccgt tcctggcgtt ggggaccatc tacctgctgt tgggccgacg gcagggcgac
     1441 cggtgggcgc tgctcatcgg gctggccgcc gccttcgccg tcggggtcgt actgcaggcc
     1501 cccttccggc ggggccacac cccggccggg gccgtgctgc cccagggctc ccaggtcttc
     1561 ggggcgctcc cccgggtgct ggccgccttt gcgtcggccg ccggcgccgg cgtggtgctg
     1621 gccggggcgg cgtggagcgc ggtgcggttc tggcgggcag cgcgggaagc acgggccgcg
     1681 ccagccggga gggccgctcc agccggcgcc ccgccgtcgg gtccccccgc cggccggatg
     1741 gcgaccgcca acgccatgat cgccttgggc accctgatcc tcggggcgag cggcctgctc
     1801 aactcggtcg ccgacgccat gaccgccttc agcgccagcc tgctcgtggg catcacggtg
     1861 ctcttcgccg ggttcctggt ggccagcccc gatcgggcgg ccacggcggc cggcccacat
     1921 ccgggccgcc tttcacccga ccctcccggc gatcccggcg ctcccggcgc tgcgaccgac
     1981 ggggcggcta cccaggaagg gcccggcgca gaaccttgcc cgccacccct tgcgggaggc
     2041 tctcgacgaa cgtcaccttc gtcgggcact tgtagcgggc cagccgcgac ccggcccagg
     2101 cgatgacgtc gtcctcctcg atggcggcgc ccctctcggg cacgacgaag gccacgaccg
     2161 tctcacccga gtaggggtgc ggccggcccg ccaccgccac ctcggcgatc cccggatggg
     2221 cgagcagcac ctcttcgacc tcggcggggt acacgttgaa ccccgacacg atgatcacgt
     2281 ccttcacccg gtccacgatg tagaggtacc cgtcgtcgtc ggccacggcc atgtcgccgg
     2341 tgcgcagcca gccgtcggcg gtcagggccc ggtcggtggc ctcggggtcg ttccagtacc
     2401 cggagaacag gttgggcccc ttcacccaga tctcgcccgg gtcgccctcc agcgcgtcct
     2461 cgccctcctc gtcgaccagc cgcacgtcca cgccgggtat gggcaccccg atcgagctgc
     2521 gccggacatc gccccccccg ccctcgcccc ccgcgccctc gccccccgcg ccctcgcccc
     2581 cagcgccctc gccccccgcg ccctcgcccc cgacgaccga ggacgtcacg accggcccgg
     2641 cctcggtcaa cccgtagccc tgccagagcg ggatgccgaa gcgggccgtg aagctgtccg
     2701 ccacctcgtc ggtgagcgcc gccgcgccgg agacggctag ccgcacggac cgcagctccg
     2761 ctccggtcgc gcccggcacg gcggccaggg cggcgaacat cgtcggggac ccgccgacga
     2821 ccgtcacccc ccgggcggcg atcgtctcga gggcggcgtc gggctcgaaa cgctcgacca
     2881 gcacgaccga cgcgcccgcc agcaacgcga ggtcgagcat cacgttgagc ccgaagatgt
     2941 ggaacatcgg cagaaccccg aggacaacgt cgtcggcccg gaccgtgcgc cccggatggc
     3001 gctggacctg ctcgaggttg gcgagcaggg cgccgtggct gagcatcgcc ggcttgggcg
     3061 cgccggccgt cccggcggtg aagatcatga ccgctatgtc cccgtccgcc cgctccaccg
     3121 cgggcgcggg cgacccggcc atgaccgatt cgagcgtgac cgccccgtcc acggacgccc
     3181 cctcggcggc gcccacgacc acctgctcga ggcccaccgc ggcccggtcg acgccgctca
     3241 gggccgccac ggccgaaggg ctggcgatgg ccaggcgggc gcgcaccgcg gcgagctcgc
     3301 cgtggagctc ggccgtcggg ctggtggggt tgagcgggac gatcgccgcg ccggtgccga
     3361 gcgcggccag gtagctgacc acgaagtacc agttgttggc ggcgaggagg gcgacgcgtt
     3421 cacccggcct gaccccgagg ccgaccagcc cgccccgcag ccggcccacc tgctcgacca
     3481 gctcgccgta ggtggtgacc tggccgcggc tgacgagggc cgcggcgccg tcggggtggc
     3541 cgtcgaggat ggtcgacagg ttggccatca ggcgagcacc gggccgagcg ggctgaacgg
     3601 gatcacgggg cgcaccgggc tcaccggagg acgaggaact ggacaagcgc ggccacgaag
     3661 agggcggcca gcaggaccgc cagggtgatg gtggtcgccg gcctcacggc gctcatgctg
     3721 gcacagcccg ccgccgcccg gcaccccggc tcaggaggcc tcgccgatcg ccacctcgtc
     3781 gccggcgcgc aacccgagct ggtccgcggc cgaagcctgg tccatgctga gcgcgagcag
     3841 cccgtaggag tcgacgagga cgccgatctc gcccggtccc agctcggcat aggcgccgca
     3901 gcgccggccg gtgcgaagct ggtccttgca ccgcagggcg aggcgctcgc cgaaggcggt
     3961 gacctcctcg ggcccgacgt tgagctgggc gttgccgaag cggtccaccc acagcacctc
     4021 ggcgagcaac cagccatcct cctggcgcgg gagggggagg aggccagggc gcagcgtggc
     4081 ggggtccagc tcctccccga gggaggccag gtcgaccccg ttgcacaggt gggcggccac
     4141 cggcgcgaag acgtcacgtc cggcgaaggt cggcc
//
LOCUS       DASWGG010000270         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1215888, whole genome shotgun sequence.
ACCESSION   DASWGG010000270 DASWGG010000000
VERSION     DASWGG010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215888"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VF954_04795"
     CDS             complement(<1..278)
                     /locus_tag="VF954_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-binding domain-containing protein"
                     /protein_id="HEX9530434.1"
                     /translation="MAVPFLAATAPSATSAAFPLLTTLVLLPAVAALIVTLLPRTRPE
                     LVKLVGFLAALAEAALCVVLLWQFRAGEGGFQLVTQHSWISDFGISWKL"
     gene            complement(278..>1878)
                     /gene="nuoL"
                     /locus_tag="VF954_04800"
     CDS             complement(278..>1878)
                     /gene="nuoL"
                     /locus_tag="VF954_04800"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX9530435.1"
                     /translation="GFPRFFVYLNLFAFSMLMLVLGNSFLVMFLGWEGVGACSYWLIS
                     FWFQRDRPPPAGKKAFVTNRIGDFGFMLAIFLVFQHTGSLQFDQVFTHLGGMTRGTAT
                     AICLLFLLGAAGKSAQLPLYLWLPDAMEGPTPVSALIHAATMVTAGVYLMCRINPLLA
                     LAPGAATTVAWIGAATALFAATIACAQDDIKRVLAYSTISQLGYMFLAVGCGAYVSGV
                     FHMVTHAFFKALLFLGAGSVIHALHDEQNLKRMGGLRKLMPVTYGTFLVAWLAISGIP
                     PFAGFWSKDDILLNAWDKSIALWAIGLVTAGLTAYYMSRQVFLAFYGDERWRHAAPAE
                     AADAHLEGPGAPAPHAADGDPHESPWTMSGPLVVLAALAAAGGALNLPFVARRYQLLD
                     SWLGPVFAERLHQVHRGTDFKIILAAAATAAAAAGIAGAWGWLSRAERPELEPVVLRR
                     AWLYDEAVSAFVDGPGIAAAGFTADVVDHEIVDGAVDGVGTLVRAASTTLRRVQTGYV
                     RNYALGVAAGAVLLLGYAVLWAGR"
BASE COUNT          327 a          594 c          734 g          223 t
ORIGIN      
        1 agcttccagg agatgccgaa gtccgagatc cagctgtgct gggtgacgag ctggaagccc
       61 ccctcccccg ccctgaactg ccacaggagc acgacgcaca aggcggcctc ggccagggcg
      121 gccaggaagc cgaccagctt gacgagctcg ggtcgggtgc ggggcaggag ggtcacgatc
      181 agggcggcga ccgccggaag caggaccagg gtggtgagga ggggaaaggc ggccgaggtg
      241 gcagagggcg ccgtggccgc gaggaagggc actgccatca ccggcccgcc cacagcacgg
      301 cgtagccgag gagcagcacg gcgcccgccg ccaccccgag ggcgtagttg cgcacgtagc
      361 cggtctggac ccggcgcagc gtcgtgctgg ccgcccgcac gagcgtgccg accccgtcga
      421 cggcgccgtc gacgatctcg tggtcgacga cgtcggccgt gaagccggcg gcggcgattc
      481 cggggccgtc cacgaaggcg ctcaccgcct cgtcgtagag ccatgcccgg cgcagcacga
      541 cgggctcgag ctcggggcgc tccgcccggc tgagccagcc ccacgccccc gcgatgccgg
      601 ccgccgccgc cgccgtggcc gccgccgcca ggatgatctt gaagtcggtg ccgcgatgga
      661 cctggtggag ccgttcggcg aagacgggcc cgagccagga gtcgaggagc tggtaccgcc
      721 tggccacgaa gggcaggttc aacgcgccgc cggcggcggc gagggcggcc agcacgacga
      781 gcgggccgct catggtccag ggcgactcgt ggggatcgcc gtcggcggcg tggggggcgg
      841 gggcgcccgg cccttcgagg tgggcatcgg ccgcttcggc gggcgcggcg tggcgccacc
      901 gctcgtcccc gtagaaggcg aggaacacct ggcggctcat gtagtaggcg gtgaggccgg
      961 cggtgacgag cccgatggcc cacagggcga tgctcttgtc ccatgcgttg aggaggatgt
     1021 cgtccttgga ccagaacccg gcgaagggag ggatgccgga gatggccagc caggcgacga
     1081 ggaaggtgcc gtaggtgacc ggcatgagct tgcggagacc gcccatgcgc ttgaggtttt
     1141 gttcgtcgtg gagggcatgg atgaccgacc cggcaccgag gaagagcagc gctttgaaga
     1201 aggcgtgggt gaccatgtgg aacacgccgc tcacgtaggc gccgcagccc acggccagga
     1261 acatgtagcc cagctgcgag atcgtcgagt aggccaggac ccgcttgatg tcgtcctggg
     1321 cgcaggcgat ggtcgccgcg aacagcgcgg tggcggcgcc gatccaggcc acggtggtgg
     1381 ccgcgccggg ggccagcgcg agcagcgggt tgatgcggca catgaggtag acgccggctg
     1441 tgaccatggt ggcggcgtgg atgagggccg acaccggggt ggggccttcc atggcatcgg
     1501 gcagccagag gtacagcggc agctgggcgg acttgcccgc cgccccgagc aggaacagca
     1561 ggcagatggc ggtcgcggtc ccccgggtca tcccgcccag gtgcgtgaag acctgatcga
     1621 actgcaacga gccggtgtgc tggaacacga ggaagatggc cagcatgaag ccgaagtcgc
     1681 cgatgcggtt ggtgacgaag gccttcttgc ccgcgggcgg gggccggtct cgctggaacc
     1741 agaacgagat cagccagtag gagcaggcgc ccaccccctc ccaaccgagg aacatcacga
     1801 ggaagctgtt gcccagcacc agcatgagca tcgagaaggc gaacaggttg aggtacacga
     1861 agaagcgggg aaagccgg
//
LOCUS       DASWGG010000271         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_437485, whole genome shotgun sequence.
ACCESSION   DASWGG010000271 DASWGG010000000
VERSION     DASWGG010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437485"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VF954_04805"
     CDS             <1..398
                     /locus_tag="VF954_04805"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX9530436.1"
                     /translation="GHTGHIKGVLTDQAVVAGVGNAYSDEALHAAKLSPYRSAARLEE
                     EEIGRLHGSLVGVLRDAVGRAAGLPAKGLKSEKKSGLRVHGRAGQPCPVCGDDIREVS
                     FAAKSFQYCPTCQTGGKALADRRLSRLLK"
     gene            451..>1655
                     /locus_tag="VF954_04810"
     CDS             451..>1655
                     /locus_tag="VF954_04810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9530437.1"
                     /translation="MQRLSGLDAGFLYLETPNNHMHVASTAVFDPSTMPGGYSFEKVR
                     QLVLERLPLLPPFRRRLAEIPFGLHHPLWIEDPDFDLDYHVRRAALPAPGGKRELADL
                     AAELMGRPLHRERPLWELHVVEGLEYGMIAIVTKTHHAAIDGVSGAELTVNLLDLTPE
                     VAPPPVEDPPWQPDHVPTDFELVAYALSSLARQPVHGLRVARRTFETALRLRRRNRQP
                     NVTPPPAPFSAPRTSLNTSITPHRRFAFTTVSLDEVREVKNAFGGTVNDVVLAMCAGT
                     LRRYLDDRDEHPDGALVAMVPVSIRTEDEKGSMGNKLSPMLVSLATDIDDPVERLRAI
                     SEGTVAAKEQERAIGAATLTDWVEFMAPAVAARAARLASSTKIFDRVRPLYNVTISNV
                     PGPKFPLYSA"
BASE COUNT          254 a          605 c          558 g          238 t
ORIGIN      
        1 ccggccacac gggccacatc aagggggtgc tgaccgacca ggccgtggtg gcaggggtgg
       61 ggaacgccta ctccgatgag gccctgcacg cggccaagct ttctccctac cggtccgctg
      121 ccaggctcga agaggaggag atcggccgcc tgcacggcag cctcgtcggc gtcctgcgcg
      181 acgccgtggg gcgcgccgcc gggctgccgg cgaagggtct caagtcggag aagaagtccg
      241 ggctgcgggt ccacggccgg gcaggtcagc cctgcccggt gtgcggcgac gacatccggg
      301 aggtctcctt cgccgccaag tcgttccagt actgcccgac ctgccagacg ggcggcaagg
      361 ccctcgccga ccggaggttg tcccgcctcc tgaagtagcg cgagctgcca gaatgggcgc
      421 agctcttacg cctgagaagg aggccagccc atgcagcgtc tcagcgggct cgacgccggg
      481 ttcctgtacc tggagacgcc taacaaccat atgcatgtgg cttccacggc ggtgttcgac
      541 ccgtcgacca tgcccggcgg gtactccttc gagaaggtcc gccagctggt gctggagcgg
      601 ctcccgctcc taccgccgtt ccggcgccgg ctcgccgaga tccccttcgg tctgcaccat
      661 ccgctctgga tcgaggaccc cgacttcgat cttgactacc acgtccgccg ggccgcgctg
      721 cccgcgccgg gaggcaagcg ggagctggcc gatctggcgg ccgagctaat gggtcgcccg
      781 ctgcaccgcg aacgcccgct ctgggagctg cacgtggtcg aggggctcga gtacggcatg
      841 atcgccatcg tcaccaagac ccaccatgcc gccatcgacg gcgtgtcggg cgccgagctc
      901 accgtcaacc tcctcgatct cacgccggag gtggcccccc cgccggtcga agacccgccg
      961 tggcaaccgg accacgtgcc gacggacttc gagctggtgg cctatgccct cagctccctg
     1021 gcccgtcagc cggtccacgg gctcagggtg gcccgccgca ccttcgagac cgcgctccgc
     1081 ctgaggcgcc ggaaccgcca gcccaacgtg acgccgcctc cggctccgtt cagcgcgccc
     1141 cggacctcgc tcaacacctc gatcacccca caccggcgtt tcgccttcac gaccgtcagc
     1201 ctggacgagg tccgggaggt gaagaacgcg ttcggcggca cggtcaacga cgtggtgctc
     1261 gccatgtgcg ccgggacgct gcgccgctac ctcgacgacc gggacgagca ccccgacggc
     1321 gccctggtag ccatggtgcc cgtgtcgatc cggaccgagg acgagaaagg atccatgggg
     1381 aacaagctgt cgcccatgct cgtgtcgctc gccaccgaca tcgacgaccc cgtcgaacgg
     1441 ctgcgggcca tcagcgaggg caccgtcgcg gccaaggagc aggagcgggc catcggcgcg
     1501 gcgacgctga cggactgggt ggagttcatg gcgccggcgg tggcggcgcg ggcggcccgg
     1561 ctggcgtcgt cgaccaagat cttcgaccgg gtcaggccgt tgtacaacgt gaccatctcc
     1621 aacgtgcccg ggcccaaatt cccgctgtac tcggc
//
LOCUS       DASWGG010000272         4383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_887253, whole genome shotgun sequence.
ACCESSION   DASWGG010000272 DASWGG010000000
VERSION     DASWGG010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887253"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..497)
                     /locus_tag="VF954_04815"
     CDS             complement(294..497)
                     /locus_tag="VF954_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530438.1"
                     /translation="MPWCEGCDRFYNPTSLGKVGECPTCGRVLAKPPSPAAPWHFKLM
                     IVAVSVYLAYRLVQGVVWIAHHA"
     gene            complement(525..2084)
                     /locus_tag="VF954_04820"
     CDS             complement(525..2084)
                     /locus_tag="VF954_04820"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX9530439.1"
                     /translation="MPDADADADGDPLARLASAMVRARAGNLAREGEKLTRQHKLFVR
                     DRIALLVDPGTFVEDGLLANALAPAGDLPADGVVTGRGLVDGRPACVMANDPTVKAGS
                     WGARTVEKIVRLTEYALSHEVPVFWLVDSAGARITDQVELFPGRRGAGRIFANQVRLS
                     GKVPQICCLFGPSAAGGAYIPAFCDVVFMVEGNASMYLGSPRMAEMVIGESATLEEMG
                     GARMHATVSGCGDNLVADDRGAIEAARQYFSYLPGSWQEDPPHEPAAEPATRLTASTV
                     PIDVRLGYDMHGVIAGLVDEGSFFEIKPDFATEVIVGFARLEGNAVGVVASNPSRKGG
                     VLFVDSADKVARFVWCCDAFNVPLVFLCDVPGFMIGTQVERQGIIRHGAKMITAVAEA
                     TVPKVSVIVRKAYGAGLYAMAGPGFDPDACIALPSAQIAVMGPEPAVNAVYANRIAAI
                     DDLEARAEFVAARRAEYEADVDLLRLAADLVVDAVVEPEDLRDELVTRLALAAGKDRH
                     FSHRRHGVPPV"
     gene            complement(2094..3185)
                     /locus_tag="VF954_04825"
     CDS             complement(2094..3185)
                     /locus_tag="VF954_04825"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX9530440.1"
                     /translation="MLHHPVLLAVAAACMVAAYVWGRSTRRLQRACSRIQRDVMAGKA
                     SSEALVLARSTFRKELHTTVLYGLLAAMTTIAAFLADRFLELPALLILVPVAISLRYA
                     RSFMTAARLAEERSIIERRAEEVLAQEELAPLRWAARLAPEDLPQIDGFEVGRVYEPG
                     TGAMAGDFYDVHPTGGQRLAAVIGDVTGHGIEPSITAFQVKYLLRVFLRQYRDPGQAV
                     EELNQVLSASGRADELVSLAVVVFDQVAGTLRYASAGHPPAWLWHDGAVRPLRSTGPL
                     LTLDPHGSFTSREVGLEVGDVLLMYTDGLTEARSGEQLFGEDRVAAILRRDPGEDVEV
                     LCKTLLEAARDFASEPLGDDVAILAIRRT"
     gene            complement(3264..>4383)
                     /gene="der"
                     /locus_tag="VF954_04830"
     CDS             complement(3264..>4383)
                     /gene="der"
                     /locus_tag="VF954_04830"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX9530441.1"
                     /translation="LDAQVSAQAERAVRDADVILLVVDAVVGVTEEDERVAALLRRAG
                     RPVLVVANKADSEHRDPDSWLFARLGLGDPWSVSALHGRGTGDLLDAVVRHLPAERPQ
                     TAEPAPVEPEAEASPAVPAVAIAGRPNVGKSTLFNRLIGDDRSVVHDLPGTTRDTVDT
                     VVDTPEGPLRFVDTAGMRRKSRVDEGAEYYSLVRALQAIDRADAAIFVIDSTEGVTHQ
                     DQRLAERIDAAGSPIVILLNKWDLLGAEARARVTGEVEDRLAFLGYAPVLKVSARTGL
                     GTHRLLPALGEAIAAYHRRVPTGELNKAVQAAQSAHPSPAGRVLYATQGATDPPTFTL
                     FVSRPLPAPYLRYLERKIREHFGFGPTPLKLRVRRRAG"
BASE COUNT          632 a         1566 c         1614 g          571 t
ORIGIN      
        1 ttcgtcagtc aactgaaaag ccacggaaga cgttcaaaag tccgcacgtg cggactcgtc
       61 cacaggccca ccgacccgtg tggacccctt ctcgacacgc gtcgtggtcg ggccgccaca
      121 tcccacccac gagcgatgcc ggtcgacagt ccgcacgtgc ggactcgtcc acaggcaagt
      181 ggtcaccagg gtcccgagcg aggaccagct acgcgaccac gactacccag ggctggctcg
      241 acaatgcccg cgccgggctc gaaccagtag caccccggcg cgccgggcgc cgctcacgcg
      301 tggtgggcga tccagaccac gccctgcacc agccggtagg ccaggtagac cgacaccgcg
      361 acgatcatca gcttgaaatg ccagggcgcc gccgggctgg gcggcttggc cagcacccgc
      421 ccgcaggtcg ggcactcccc caccttcccg aggctcgtcg ggttgtagaa gcggtcgcag
      481 ccctcgcacc acggcatggc gtcaggcccc tgccgccgcc ggcatcatac cgggggcacg
      541 ccgtggcgac gatggctgaa gtggcggtcc ttgccggccg ccagggccag ccgggtcact
      601 agctcgtcgc gcaggtcctc cggctccacc acggcgtcga ccaccaggtc cgcggcgagg
      661 cggagcaggt cgacgtcggc ctcgtactcg gcccggcggg cggcgacgaa ctcggcccgc
      721 gcctcgagat cgtcgatcgc cgcgatgcgg ttggcataca cggcgttcac cgcgggctcg
      781 gggcccatca ccgcgatctg ggcggacggc agggcgatgc acgcgtccgg gtcgaacccg
      841 ggcccggcca tggcatacag gcccgccccg taggccttgc ggacgatgac cgacaccttg
      901 ggcacggtcg cctcggccac cgcggtgatc atcttggccc cgtggcggat gatgccctgg
      961 cgctccacct gggtcccgat catgaacccg ggcacgtcgc agaggaacac cagcggcacg
     1021 ttgaaggcgt cgcaacacca cacgaagcga gccaccttgt cggccgagtc gacgaacagc
     1081 acgccgccct tccgggacgg gttgctcgcc accaccccga ccgcgttgcc ctctaggcgg
     1141 gcgaagccga cgatcacctc ggtggcgaag tccggcttga tctcgaagaa cgacccttcg
     1201 tcgaccaacc cggcgatcac cccgtgcatg tcgtagccca gccgcacgtc gatcggcacg
     1261 gtcgacgccg tgagcctggt cgctggctcg gcggctggct cgtggggcgg atcctcctgc
     1321 cacgagcccg gcaggtagga gaagtactgc cgggccgcct cgatggcgcc gcggtcgtcg
     1381 gccaccaggt tgtccccgca acccgagacg gtggcgtgca tgcgcgcccc gcccatctcc
     1441 tcgagcgtcg ccgactcgcc gatcaccatc tcggccatgc gcggcgagcc caggtacatg
     1501 gaggcgttgc cctcgaccat gaagaccacg tcgcagaagg cggggatgta ggcgccgcct
     1561 gcggccgacg ggccgaagag gcagcagatc tgcggcacct tgcccgacag gcgcacctgg
     1621 ttggcgaaga tgcggcccgc cccccgccga ccggggaaca gctcgacctg gtcggtgatg
     1681 cgggcccccg ccgagtcgac cagccagaag accggcacct cgtggctgag ggcgtactcg
     1741 gtgagccgga cgatcttctc caccgtgcgg gcaccccagg agccggcctt gacggtgggg
     1801 tcgttggcca tcacgcacgc cggccgcccg tcgaccaggc ccctcccggt gaccacgccg
     1861 tcggccggca ggtcgcccgc gggcgccagg gcgttggcca gcaggccgtc ctccacgaag
     1921 gtgcccgggt cgacgagcag ggcgatgcgg tcccggacga acagcttgtg ctggcgggtg
     1981 agcttctcgc cctcccgggc caggttgccg gcccgggccc gcaccatggc cgaggccagc
     2041 cgggcgagcg gatcgccgtc ggcgtcggcg tcggcgtcgg gcatcaggcc gattcaggtc
     2101 cggcggatgg ccaggatggc gacgtcgtcg ccgaggggct cgctggcgaa gtcccgggcg
     2161 gcttcgagga gggtcttgca caggacctcg acgtcctcac ccgggtcgcg gcgcaggatg
     2221 gcggccaccc ggtcctcgcc gaagagctgt tcgcccgagc gggcctcggt gagcccgtcg
     2281 gtgtacatga gcagcacgtc gcccacctcc aggcccacct cccggctggt gaagctgccg
     2341 tgcgggtcga gggtgaggag ggggccggtg gagcgcagcg ggcggacggc cccgtcgtgc
     2401 cacagccacg ccggggggtg accggcggag gcgtagcgca gcgtgccggc cacctggtcg
     2461 aagacgacca ccgcgagcga caccagctcg tcggcgcggc ccgacgccga gaggacctgg
     2521 ttgagctcct ccacggcctg gccggggtcg cggtactggc ggaggaagac gcgcagcagg
     2581 tacttgacct ggaaggccgt gatcgagggc tcgatgccgt ggccggtgac gtccccgatg
     2641 accgcggcca gccgctgccc gcccgtcggg tggacatcgt agaagtcgcc ggccatcgca
     2701 cccgtgccgg gctcgtagac gcgccccacc tcgaagccgt cgatctgcgg gaggtcctcg
     2761 ggtgccaggc gggccgccca gcgcagcggc gccagctcct cctgggccag gacctcctcg
     2821 gcccgccgct cgatgatcga ccgttcctcg gccagccggg ccgcggtcat gaagctgcgg
     2881 gcgtaccgca gcgagatggc cacggggacc aggatcagca gcgccggcag ctcgaggaac
     2941 cggtcggcca agaaggccgc gatggtggtc atggcggcca gcagcccgta caggacggtg
     3001 gtgtgcagct ccttgcggaa ggtggaccgg gccagcacca gggcctcgct gctggccttg
     3061 ccggccatga cgtcgcgctg gatgcggctg cacgcccgct ggagccgccg ggtggagcgg
     3121 ccccatacat aggccgccac catgcaggcg gccgccaccg ccaggaggac tgggtggtgc
     3181 agcatcacac gcgacgctac ctggcgggcg ggccgctccg gggaggaact ttcgccgtcc
     3241 ccactgacgg tggtgggccg ggctcagccg gcccggcgcc gcacccggag cttgagcggg
     3301 gtgggaccga acccgaagtg ctcccggatc ttgcgctcca ggtaacggag gtacggggcg
     3361 ggcagcggcc tgctcacgaa cagcgtgaag gtgggcggat cggtcgcccc ctgggtggcg
     3421 tagagcaccc ggccggccgg cgacgggtgc gccgactggg ccgcctgcac ggccttgttg
     3481 agctcaccgg tcgggacccg ccggtggtag gcggcgatgg cctccccgag ggcgggcagc
     3541 aggcggtggg tgccgaggcc ggtccgggcg ctgaccttca gcaccggggc gtagccgagg
     3601 aacgccagcc ggtcctcgac ctcgccggtg acccgggcgc gggcctcggc gccgaggagg
     3661 tcccatttgt tgagcaggat gacgatcggg ctgccggctg cgtcgatgcg ctcggccagg
     3721 cgctggtcct ggtgggtgac gccttcggtc gagtcgatga cgaagatggc cgcgtcggcc
     3781 cggtcgatgg cctgaagggc gcggaccagc gagtagtact cggccccttc gtccacccgc
     3841 gacttgcgcc gcatccctgc ggtgtcgacg aaccggaggg gcccctccgg tgtgtcgacc
     3901 acggtgtcga cggtgtcgcg ggtcgtgccc ggaaggtcgt gcaccaccga ccggtcgtcg
     3961 ccgatcagcc ggttgaagag ggtggacttc cccacgttgg gccggccggc gatggcgacg
     4021 gcggggacgg ccggcgaagc ctccgcctcg ggctcgacgg gtgcgggctc ggccgtctgc
     4081 ggtcgttcgg ccgggaggtg gcgaaccacg gcgtcgagga ggtcgcccgt gccccgcccg
     4141 tgcagagcgc tgaccgacca ggggtcgccc agaccgagcc gggcgaagag ccacgagtcc
     4201 gggtcgcggt gctccgagtc ggccttgttg gccacgacca gcacgggccg gccggcccgg
     4261 cggagcaggg cggcgacgcg ctcgtcctcc tccgtgacgc cgacgacggc gtccacgacc
     4321 agcaggatga catcggcgtc gcggaccgct cgttcggcct gggcgctgac ctgcgcgtcg
     4381 agg
//
LOCUS       DASWGG010000273         3987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2813254, whole genome shotgun sequence.
ACCESSION   DASWGG010000273 DASWGG010000000
VERSION     DASWGG010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2813254"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VF954_04835"
     CDS             <1..434
                     /locus_tag="VF954_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX9530442.1"
                     /translation="AMVDEAQSLRRRRADLEDQTLTVMEELEPVDAELAAMDEQMEAL
                     DARATDALAALAESEAVIDAETAVVVEVRAVAASRLPAELSERYERLRGRLGGIGAAR
                     LANGSCTGCHLHLPATELDRIRRAPADSVLTCDQCGRILVR"
     gene            410..931
                     /locus_tag="VF954_04840"
     CDS             410..931
                     /locus_tag="VF954_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530443.1"
                     /translation="MRPHPGALRPAVLLWFAGMAIVIVWQIFHDPAIDHRLVVLGVLL
                     PDVVDGPWGGARVMHTVAASASLLAAVMVVSRRRRLLRRQLLALPIGTFLHLVLDGIW
                     TPAGVFWWPFLGWGFHRATLPSAAHPLILTLAEESAGAAALAWVWLRFGLGDRGRRAE
                     FLRTGRFAREPVR"
     gene            1001..1999
                     /locus_tag="VF954_04845"
     CDS             1001..1999
                     /locus_tag="VF954_04845"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9530444.1"
                     /translation="MIILVRHGQTAANAEGRLLGRLDPPLTELGRRQAMALAASVGRP
                     ARVITSPLGRARETAALLGLPARIPVTVDDRWIEIDYGSYDGCPLRDVPAELWARWRS
                     DSNFRPPDGESLAAVGRRVRAACEELAEEATSSDVVVVSHVSPIKAAVAWALEVDEGV
                     AWRMRLDVAAVCRLGVGPLGPSLLGFNGTGHLARVDAAPPAAPAPPEGPRSHGAAIAP
                     GDPAAEAAAVFAAVGRERAGWTSVVDGVRNGTVDLDGALGRQELAGIKAVKIIEALPG
                     VGKVKARRAMSKAGIGDRQRLGQLGPDQIDALRAALGHGEGAHGEDGPGEGSAAVT"
     gene            complement(2018..2365)
                     /gene="crcB"
                     /locus_tag="VF954_04850"
     CDS             complement(2018..2365)
                     /gene="crcB"
                     /locus_tag="VF954_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX9530445.1"
                     /translation="MAGAIGAPARYLLDSRIQGRAQSAFPWGTLLVNASGSLVLGLIT
                     GAALYHAFPAAPKVWLGTGFCGAYTTFSTFTFETLRLLEEGAIGDAVLNASLSLLSGA
                     GAAAIGLAVAAGL"
     gene            complement(2430..2879)
                     /gene="crcB"
                     /locus_tag="VF954_04855"
     CDS             complement(2430..2879)
                     /gene="crcB"
                     /locus_tag="VF954_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX9530446.1"
                     /translation="MSQGNGAGLRPRWKTGPAPADVVAAVAAGGALGAPARYEVAQLI
                     HVASGTFPWATFWTNVTGSFALGLLLILIIERFPPSRYLRPFVATGFLGAFTTFSTFA
                     VEADVLAKDGHVPIAVAYAAASLLCGLVAAWAGMLVARLVPSTTSTP"
     gene            3053..3286
                     /locus_tag="VF954_04860"
     CDS             3053..3286
                     /locus_tag="VF954_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530447.1"
                     /translation="MSEPTPIPGQRLVLADGRGALRATWHPDGGVVVISLWRGDRCFG
                     TFRAAPAEVSALIGFLANALVSGAPPSGWADTA"
     gene            complement(3316..3679)
                     /gene="rnpB"
                     /locus_tag="VF954_04865"
     ncRNA           complement(3316..3679)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF954_04865"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            3776..>3987
                     /locus_tag="VF954_04870"
     CDS             3776..>3987
                     /locus_tag="VF954_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX9530448.1"
                     /translation="MHVDLVEALAGGAVGFTVGLTGMGGGALMTPMLILLFGVSPATA
                     VSSDLLTSLVMKPVGGIVHVRRHTVS"
BASE COUNT          516 a         1345 c         1524 g          602 t
ORIGIN      
        1 aagcgatggt cgacgaagcg cagtcccttc gccggcggcg tgccgatctc gaggaccaga
       61 ccctcacggt gatggaggag ctcgagcccg tcgatgcgga gctggcggcg atggacgagc
      121 agatggaggc gttggatgcc cgggccacgg acgcgctggc cgccctggca gagtccgagg
      181 ccgtgatcga cgccgagacg gccgtcgttg tcgaggtccg ggcggtcgcg gcgtcgcggc
      241 tgcccgccga gctgtccgag cgctatgagc ggctgcgcgg ccgcctcggt ggcatcggcg
      301 ccgcccggct cgccaacggg tcctgcaccg gctgtcatct tcacctgccc gcgacggagc
      361 tggaccgcat ccggcgggcc ccggccgact cggtgctcac ctgcgaccag tgcggccgca
      421 tcctggtgcg ctgaggccgg ccgtgctgct gtggttcgcc ggcatggcga tcgtgatcgt
      481 gtggcagatc tttcacgacc cggcgatcga ccaccgcctg gtggtgctcg gcgtcctgct
      541 tcccgatgtc gtcgacggac cgtggggagg ggcgcgggtg atgcacaccg tggccgccag
      601 cgcgagcctg ttggcggccg tcatggtggt cagccggcgg cggcgcctgt tgcgccggca
      661 gctcctggcg ttgccgatcg gcacgttcct gcacctggtg ctggatggga tatggacgcc
      721 ggcgggcgtg ttctggtggc cgttcctggg atgggggttc caccgcgcca ccctgccgag
      781 cgcggcacac ccgctcatcc tcacgctggc cgaggagtcg gccggggcgg cggccctggc
      841 gtgggtgtgg cttcggttcg ggttgggcga ccgggggcgg cgggcagagt tcttgaggac
      901 gggccggttt gcccgcgagc cagtgcgatg aggccccatg gagacgaggc ccgtggcgtg
      961 atgcccgagc gggggcccgg ccgatgaggg ggcgtctcgg atgatcatcc tggtgcgcca
     1021 tggtcagacg gcggccaatg ccgagggccg gctgctcggc cgcctcgacc cgccgctcac
     1081 cgagctgggc cggcgccagg cgatggccct ggccgcctcc gtcgggcggc ctgcccgcgt
     1141 gatcacaagc cctctcgggc gggcgcggga gacggctgcg ttgctgggtc tgcccgcccg
     1201 aatccctgtc accgtcgacg accgctggat cgagatcgac tacggcagct acgacggctg
     1261 cccgctcagg gacgtgccgg ccgagctgtg ggcccgctgg cgctcggact ccaacttccg
     1321 cccgccggac ggcgagtcgc tcgccgcggt cggccgcagg gtccgggccg cgtgcgaaga
     1381 gctcgccgaa gaggcgacct cgtcggacgt ggtggtggtg agccacgtgt cgcccatcaa
     1441 ggccgcggtg gcatgggccc tcgaggtgga cgaaggggtg gcctggcgca tgcgcctcga
     1501 cgtcgccgcg gtgtgccgcc tcggcgtcgg cccgctcggc ccttcgctcc tggggttcaa
     1561 cggcacgggc catctggcca gagtcgacgc ggccccgccg gccgccccgg ccccgccgga
     1621 gggaccacgc tcgcacggcg ctgccatcgc gcccggcgac cccgcagctg aggccgcggc
     1681 tgtcttcgcc gcggtgggcc gggagcgggc gggatggacc tcagtggtgg atggggtcag
     1741 gaacgggacg gtcgatcttg acggtgcgct cgggcggcag gagctggcgg gcatcaaggc
     1801 cgtcaagatc atcgaagccc tgcccggggt cgggaaggtg aaggcccggc gggccatgtc
     1861 gaaggccggg atcggagacc gtcagcggct cgggcagctg ggcccggacc agatcgacgc
     1921 cctgcgggcc gccctggggc acggggaagg ggcgcacggg gaagatgggc ccggggaggg
     1981 gtcggcagcg gtgacgtgat cctttccccc gagatggtca caaacctgca gccaccgcca
     2041 ggccgatcgc cgccgcaccc gcgcccgaga gaaggctgag ggaggcgttg agcacggcat
     2101 cgccgatcgc gccctcctcg agcagccgca gcgtctcgaa ggtgaaggtc gaaaacgtcg
     2161 tgtaggcacc acagaagccg gtgccgagcc agaccttcgg tgcggccggg aaggcgtggt
     2221 acagcgccgc cccggtgatg aggcccagga ccagggatcc cgaggcgttg acgagcaacg
     2281 tcccccaggg gaaggcgctc tgggctcggc cctggatacg tgagtccagc aggtagcggg
     2341 cgggggctcc gatcgctccg gccactacca gcccagccca ggtggccgcg ccggtcacga
     2401 cgaggccagc ggcgggcggc cgacgggcgt cacggcgtcg acgtcgtcga cgggacgagg
     2461 cgagccacca gcatgcctgc ccacgctgcc acaaggccgc acagcaggct ggcggcggcg
     2521 taggccacgg cgatggggac gtggccgtct ttggccagca cgtcggcttc gacggcgaac
     2581 gtcgagaacg tggtgaaggc cccgaggaag ccggtggcga cgaagggccg aaggtaccgg
     2641 ctcggtggaa agcgctcgat gatcaggatg aggaggagcc ccagggcgaa gctgccggtc
     2701 acgttcgtcc agaacgtggc ccaggggaag gtgccgctgg ccacatggat cagctgggcc
     2761 acttcgtacc tcgcgggcgc gcccaacgcg ccgcctgcgg cgacggcggc cacgacgtcg
     2821 gcgggcgcgg ggcccgtctt ccacctgggg cgcaatcctg cgccgttgcc ctgtgacatc
     2881 gctccacctc cctgacctgg gtgctgtcta gcgccggttg tcctctcttg ttgcccttgt
     2941 ctgtagaaaa ggcgaccgac gcgccctctc cgccgtcggg gcggactcga ccggcgccag
     3001 ataggcctga gggtcgatgg acactctccg ggcggcggtc catcatgggc atgtgagcga
     3061 gccgaccccc attccgggcc agcgtctggt gctggcggac gggcggggcg cgttgcgtgc
     3121 gacctggcat cccgatggcg gcgtggtggt catcagcctg tggcgtgggg accggtgctt
     3181 tggcacgttc cgggccgccc cggccgaggt ctcggcgttg atcgggttcc tggccaacgc
     3241 gctggtctcc ggcgccccgc caagtggctg ggccgatact gcgtagtcgg acgcggtttg
     3301 gcagggggcg tggtgacggt gagccggcct gtaagcgggg ttctgtcgcc cggtccgttg
     3361 ccgggccggg cgggtggcca tccatctaag cggcctacct gaggacatcg gccgggccag
     3421 ccgtcctcat cttggccttg ctccgggtgg ggtttgccga gccgtccggg tcgccccggg
     3481 cgctggtgcg ctcttaccgc accgtttcac ccttacctgt ggccgccccg gatgcccggg
     3541 gatcggccca tcggcggtct gttctctgtg gcactttcct gcgggtcgcc ccgactggcc
     3601 gttagccagc accttgccct atggagcccc gactttcctc gacacgatca gctcgtgccg
     3661 cggccacccg gccggctcac cgtcaccgct cattgtcgca cgcgcccggc gacgccgcga
     3721 cacggtctta tgtaacaaag ttgacggaga aagtcgggaa tggtagaccc gggcggtgca
     3781 cgtcgacctg gtcgaggcct tggcaggcgg ggccgtcggc ttcaccgttg gcctgaccgg
     3841 catgggcggc ggggcgctca tgacccccat gctcatcttg ctcttcgggg tcagcccggc
     3901 gaccgcggtg tcgagcgacc tgctgacgtc gctggtgatg aagcccgtcg gcgggatcgt
     3961 gcacgtgcgc cgccacacgg tgagctg
//
LOCUS       DASWGG010000274         3449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1217152, whole genome shotgun sequence.
ACCESSION   DASWGG010000274 DASWGG010000000
VERSION     DASWGG010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217152"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VF954_04875"
     CDS             complement(<1..302)
                     /locus_tag="VF954_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530449.1"
                     /translation="MKLQAPRATGLARPRLGDALAGAWEHRLSLVTAPAGSGKTTLLA
                     QFAAAAPVPVAWYRAESTESGADRVLDHIAAALEATDPRWAGLPSSSPEALASGLG"
     gene            complement(556..1335)
                     /locus_tag="VF954_04880"
     CDS             complement(556..1335)
                     /locus_tag="VF954_04880"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEX9530450.1"
                     /translation="MIHEADESLVALFADAEFGYKSPAFSFEAPTAEWRGKQAAGPVV
                     NLFLHHVVEMVESRVASSRTVRDTDGRVSERRPPVRRYELAYLVSAWAANVEEEHRLL
                     GDVLRHLAEWESLPPQYRRGSLAQAELPIPMFVGTPVTGVAPANRWDIWGTLGGGAKT
                     SFDLVLSVPLIPRPDVDIGPPAESRRFDFGLVGTKAPGDNRESLVSPNRDTTAGARNA
                     EQHQAEVEAERRAAEKANPPAPAGKVRSRIVREGPAPAQER"
     gene            1523..3037
                     /locus_tag="VF954_04885"
     CDS             1523..3037
                     /locus_tag="VF954_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEX9530451.1"
                     /translation="MPTYLAPGVYMEEVSSGSKPISGVGTAVAAFVGLAEKGPVNSPT
                     LVTNWTQFVENFGEFVEGSYMAHSVYGYFLNGGGVAYVVRVGANGAADKPAARAELPS
                     GADKAVSAYKISALEPGATGDDISVEIADPAEAGDDNFKLIVRKGQKVEETFDTVSTK
                     KGPINVVTQVKQKSKLIKVEESGGTVAVPEKGRFPLGGGNAPVPVNLSPDAYVGSSAD
                     RTGFGGLEAIDEITMLSVPDLMAAYQQGVIDMEGLKAVQLAMIAHCELMNNRLAILDP
                     PPGLNAQQIKEWRMDLAGYDSKQAALYWPWIKVMDPLKGEAVFMPPSGHMAGIWARTD
                     NTRGVHKAPANEIVRGAITLELQITRGEQEQLNPNGVNCIRSFPGKGIRVWGARTLSS
                     DPEWRYVNVRRLFNFVEQSILNSTDWVVFEPNDFRLWISVQRNVIAFLRNVWRSGALF
                     GEIENAAFWVKCDAENNPPESRDVGELHIDIGIAPVKPAEFVVFRIQQIASGGE"
     gene            3078..>3449
                     /locus_tag="VF954_04890"
     CDS             3078..>3449
                     /locus_tag="VF954_04890"
                     /inference="COORDINATES: protein motif:HMM:NF018543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX9530452.1"
                     /translation="MATPDVITAARFELNVNELGQAEFSELGGISMEIDVVEFQDNDA
                     QGMIRLHKLPGKAKPATITLKRPMNGTMLLWEWHASINNFSHKAEMPLSKAQRGGTLK
                     MYSMEGGEPVAVYEFLGGWPSK"
BASE COUNT          552 a         1200 c         1218 g          479 t
ORIGIN      
        1 tcgccgaggc ccgacgccag ggcttcgggc gacgagctgg gcaggccggc ccagcgagga
       61 tcggtcgcct ccagggcggc ggcgatgtgg tcgagcaccc ggtcggcgcc gctctcggtc
      121 gactcggcgc gataccacgc caccgggacg ggcgcggcgg cggcgaactg ggcgagcagg
      181 gtggtcttgc cggaccctgc cggcgccgtt accaggctca ggcgatgctc ccaggcgccc
      241 gccagggcgt cgccgagtcg agggcgggcc aacccggtgg cgcggggggc ctggagcttc
      301 accgcgagca ccggtggggc ggggggccgc gccgcgaggc gccctggtcc cgccgccgca
      361 tcgtgctgag ctggctgtac cacctgtgtc gcctccgcca ctccccggac cttggataac
      421 gacgctgcct tctgccgata ccgtacacgg gcaggccgcc cgcgggcgcg aacgggcagc
      481 cggatgtgcc cggaaggccg ggccggcctg ccccagtcgt cttcggggcg tcgggggaaa
      541 ggccgacccg gtgccctacc gctcttgcgc cggcgccggg ccctcgcgca cgatccggga
      601 gcggaccttg cccgccgggg ctgggggatt ggccttctcg gccgcccggc gctcggcttc
      661 cacctcggcc tggtgctgct cggcgttgcg agcgcccgcg gttgtgtccc ggttgggcga
      721 gaccagggac tccctgttgt ccccgggcgc cttggtgccg accaggccga agtcgaagcg
      781 ccgggactcg gcggggggcc cgatgtcgac gtcggggcgg gggatcaacg ggacggagag
      841 gaccaggtcg aaggacgtct tggcgccgcc gccgagcgtg ccccagatgt cccaccggtt
      901 ggccggggcc acgccagtca cgggcgtgcc gacgaacatg gggatcggca gctcggcctg
      961 ggccagagag cccctccggt actggggcgg cagcgactcc cactcggcga ggtggcgcag
     1021 gacgtcaccg aggaggcggt gctcctcctc cacgttggcc gcccacgccg acaccaggta
     1081 ggccagctcg tagcggcgga ccggcgggcg gcgctccgag acccggccgt ccgtgtcccg
     1141 caccgtccgg gagctggcca cgcggctttc gaccatctcg accacgtggt ggaggaacag
     1201 gttcacgacc ggtccggcgg cctgcttacc ccgccactcc gcggtgggtg cctcgaagct
     1261 gaacgccggg ctcttgtagc cgaactcggc gtcggcgaag agggcgacaa gggactcgtc
     1321 cgcctcgtgg atcatcgccg cccaccatcg cgcccgggcg ggcgcggggc aaccaccagc
     1381 tggctgccga ggtgtccgtc ccaggggacg gctgcccgaa cgggcaccgg tccgcgccct
     1441 cgtggctggc cgaagggcgc taccgcgatc gagtcccgcc cgccacgctg gcgttcgtcc
     1501 atctggcacg aggagcttgc ccatgccgac ttatcttgcg cccggcgtct acatggagga
     1561 ggtctcctcc ggctcgaagc ccatctcggg tgtcggcacg gcagtggccg ccttcgtcgg
     1621 tctggccgag aaaggccctg tcaacagccc gaccctggtc acgaactgga cgcagttcgt
     1681 cgagaacttc ggcgagttcg tcgaggggtc ctacatggcc cactcggtct acggctactt
     1741 cctcaacgga ggcggcgtgg cctacgtcgt ccgcgtcgga gccaacggcg cggcggacaa
     1801 gcccgccgct cgggccgagc tgccgagcgg cgccgacaag gccgtttccg cctacaagat
     1861 cagcgcgctg gagcccggag ccacgggcga cgacatcagc gtggagatcg ccgacccagc
     1921 ggaggcggga gacgacaact tcaagctgat cgtgcgcaag ggccagaagg tcgaggagac
     1981 cttcgacacg gtgtccacca agaagggccc catcaacgtc gtcacgcagg tcaagcagaa
     2041 gtccaagctg atcaaggtcg aggagagcgg cggcacggtc gccgtcccgg agaagggccg
     2101 cttcccgctc ggcggcggca acgcccccgt cccggtgaac ctgagccccg acgcctacgt
     2161 gggcagctcg gccgatcgca ccggcttcgg cggcctcgag gccatcgacg agatcaccat
     2221 gctctcggtc ccggacctca tggccgccta ccagcagggc gtcatcgaca tggagggcct
     2281 gaaggccgtc cagctggcga tgatcgccca ctgcgagctc atgaacaacc gcctggccat
     2341 cctcgacccc ccgcccgggc tgaacgccca gcagatcaag gaatggcgga tggacctggc
     2401 cggctacgac tccaagcagg ccgccctgta ctggccgtgg atcaaggtca tggacccgct
     2461 gaagggcgag gcggtgttca tgccgccgtc gggccacatg gccggcatct gggcccggac
     2521 cgacaacacg cggggcgtgc acaaggcccc ggccaacgag atcgtgcgcg gcgccatcac
     2581 cctggagctg cagatcaccc ggggcgagca ggagcagctg aaccccaacg gcgtgaactg
     2641 catccgctct ttccccggca agggaatccg ggtgtggggc gcccggacac tctcgagcga
     2701 cccggaatgg cgctacgtca acgtgcgccg gctgttcaac ttcgtggagc agtcgatcct
     2761 caactccacc gactgggtcg tgttcgagcc caacgacttc cgcttgtgga tctcggtgca
     2821 gcgcaacgtc atcgccttcc tgcgcaacgt gtggcggtcg ggggcgttgt tcggcgagat
     2881 cgagaacgcc gccttctggg tgaagtgcga cgccgagaac aacccgcccg agagccgcga
     2941 cgtgggcgag ctgcacatcg acatcgggat cgcgccggtc aagccggcgg agttcgtggt
     3001 gttccgcatc cagcagatcg cctccggcgg cgagtagccg accggccacc cacacgatcc
     3061 atagatagga gacagacatg gcgacgcccg atgtcattac cgctgccaga ttcgagctca
     3121 acgtgaacga gctcggccag gccgagttct ccgagctcgg cggcatctcg atggagatcg
     3181 acgtggtcga gttccaggac aacgacgccc agggcatgat ccggctgcac aagctgccgg
     3241 gcaaagccaa gccggcgacc atcacgctca agcggcccat gaacggcacc atgctgctct
     3301 gggagtggca cgcgtccatc aacaacttca gccacaaggc cgagatgccg ctctcgaagg
     3361 cccagcgggg cgggacgctg aagatgtaca gcatggaggg aggcgagcct gtggccgtct
     3421 atgagttcct cggcgggtgg ccgtccaag
//
LOCUS       DASWGG010000275         7990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3147335, whole genome shotgun sequence.
ACCESSION   DASWGG010000275 DASWGG010000000
VERSION     DASWGG010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3147335"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VF954_04895"
     CDS             complement(<1..262)
                     /locus_tag="VF954_04895"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530453.1"
                     /translation="MPVPGATHGDGLPPELVGWLAGTRFGDVRWFDTIDSTNRYVLDQ
                     ARSGAPEGAVAVAEHQTAGRGRMGRSWVAPPEASLLLSVLLRP"
     gene            316..2079
                     /locus_tag="VF954_04900"
     CDS             316..2079
                     /locus_tag="VF954_04900"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9530454.1"
                     /translation="MLTKVLIANRAEIAVRVIRTCRELGIATVAVYSELDRDALHVRL
                     ADEAYALGGQTAAESYLNTTAIIDALNRSGADGVHPGYGFFAENADFARAVQAAGVTW
                     IGPPPAAIEVMGDKVSSRLAAARAEVAAVPGTTEVLTGPEDVVAFGTTHGWPVAIKAA
                     YGGGGRGMKVVPSADAAAELLESARREAKAAFGRDECYLERYLTWPRHIEMQIFGDTH
                     GNYVWLGERDCSSQRRHQKLIEESPAPLFPEAVRVAMGEAAVKVAAACGYQNAGTVEF
                     MFQDGEFFFLEMNTRLQVEHPVTEQVVGRDLVELQLRIAAGEPLGFDQADIEHHGHAI
                     ECRINAEDPAGGRFVPSPGTISRFRRPDGYGVRTDTGYDEGDTVSQYYDNLIAKLIVW
                     GQDRERARRRMIRALEEMEVEGVATTVPAHLAILRHPDFIESKHSTRWVEDVLDLSSL
                     NPPPAPVPPPDDEPRVLREVDAEVDGRRYKVRLWVPDVAPALAVGGPAPARATRSRPA
                     AAAAAAGSGSGNVTVPMQGTIVKVLVVAGDEVEAGQAVCVLEAMKMENNISAERSGRI
                     TEVKVVPGDTVGAGDIVAVIA"
     gene            2113..3288
                     /locus_tag="VF954_04905"
     CDS             2113..3288
                     /locus_tag="VF954_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX9530455.1"
                     /translation="MQASEAKDAARRSLGSAADALVALSHTIHAHPELAFEEERSSGW
                     VADALAGAGFAVETGVGGLPTALAATAGSGPLTLAICAEYDALPGIGHACGHNIIAAA
                     AVGAGLALAPIADDLGLTVKVMGTPAEEGGGGKIIMLERGVFDGVNASMMVHPAPLEQ
                     AQMHCLAVSHFDVHYTGKEAHASAFPERGVNAGDALTVAQVALGLLRQHVDTGDQFHG
                     IVTKGGEAPNIVPAHTSAKYYVRASSLERLAHIEPRVRRCFEAGALATGATLAIKPAS
                     PPYSEFLADQQLLDTYVRNASALGRRFSATDGGLRGGSTDMANLSLAMPAIHPMLGIE
                     SLPAVNHQPEFAAACITPPADQAVTDGALAMAWTAIDLALEPEPRARLLAFSVGAGP"
     gene            complement(3297..4196)
                     /locus_tag="VF954_04910"
     CDS             complement(3297..4196)
                     /locus_tag="VF954_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9530456.1"
                     /translation="MSRMPAVSLVAVPGRRNRTVELARQIEDRGFTGVYCPSIMGDAI
                     SLCVSMAHATERLEFGTSIQPIYLRRPQELAGAASYLHEVSGGRFRLGLGVSHAPAMQ
                     RMGVETGRPLGDTRDYVGSLRAAEKQVGPLPPIVLATLRDKMLALATEISDGAVWANA
                     ARSHMAAQLSAVEVGGEFFVGNMIPTVIDEDREAAAAVNRKSLTGYLALPNYRNYWKA
                     AGYVEEMEAIEAALAAGDRDRLPALMTDRWLSDCTLYGSAAQVREGVEAWIEAGVKAP
                     ILVPSSTSGGQLKALEELFAAYD"
     gene            complement(4220..5584)
                     /locus_tag="VF954_04915"
     CDS             complement(4220..5584)
                     /locus_tag="VF954_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9530457.1"
                     /translation="MPVSLAIIGGGPAGVQAATHAARLGADVTVIERDIVGGAAHLWD
                     CIPSKAMIAAGGIMNLSRRAEQLGLGALQPTLDPAALKDRIAGVSRHLERSVTELLSS
                     QGVEVVRGTARLVSPHRLVADTEEGARELDADAVLLSTGSRPRIPPWADIDGERVLTT
                     RDAYPPKELPAHLVVIGSGVTGVEFTHMFRSLGARVTLIVSRQQVLPNKDPEVAAALE
                     NDFISTGVRLLKGARAEAIERTEDGGVAVRCDDGRVAPGSHALLCIGSLPNSEGLGLE
                     AAGVEVDAGGYVPVNQHCQTNVPHVYAAGDVSGRLPLASVAFMQGRKVAEHVMGLHTR
                     EHRHLDYDKAASAIFTVPEIADVGLAEAEAFAMGRKIRVTKVPFAANARALIDGDPRG
                     FVKILSDPATGVVLGGSIVGRRAAELISVIALAVTANLKVTDIVESLLVHPALAEALA
                     DAAE"
     gene            complement(5609..7192)
                     /locus_tag="VF954_04920"
     CDS             complement(5609..7192)
                     /locus_tag="VF954_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9530458.1"
                     /translation="MARDPRRTDSGIEIQPVYAAGDLAGWDPATKLGEPGSYPYTRGI
                     RKDLYRGRLWTMRQYSGFGNAEATNERWKQLTAAGSTGLSCAFDLPTQMGYDSDHPRA
                     EGEIGKVGVAIDSVEDMRLLLADLPLDQVTTSMTINATAAILLLLYQLVAEEHGVPAA
                     RVGGTIQNDVLKEYIARGTYIYPPRPSMRLVTDIFAYCRENLPSWNTISISGYHIREA
                     GSTAVQEIAFTIANGIAYVQAALDTGLAIDEFAPRLSFFWNAHNNFFEEVAKYRAARR
                     MWARIMTDRFGARDAKSKLLRFHTQTGGSTLTAQQPENNIVRVTLQALAAVMGGTQSL
                     HTNGFDEALGLPTQRAATMALRTQQVVGFESGVADTVDPLGGSYFVERLTDEVEDAAW
                     AYIDKIDAMGGAVAAIEAGYMQDEIEQAAYAYTKAVDDGEKVIVGVNQYATDEVQDAD
                     VFPIDPELARRQVERTRRVRAERDQAGVDAALADVAAAARGSQNLLVPMKEALSRRAT
                     LGEVSDVLRGEFGVYQPSR"
     gene            complement(7286..7570)
                     /locus_tag="VF954_04925"
     CDS             complement(7286..7570)
                     /locus_tag="VF954_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530459.1"
                     /translation="MGALRAGNNAVGDQTFERRGGRHAEEARYRDPAVRDDHLLALAG
                     SFKPVTQVRPQLAHGYIHATSVQHIVPLSVRRRPAPDPRRPVDESARADF"
     gene            7652..>7990
                     /locus_tag="VF954_04930"
     CDS             7652..>7990
                     /locus_tag="VF954_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9530460.1"
                     /translation="MPLVYFDSSALVKLLVEEAGSELAAEIWDGCDAAIASRLAYPEV
                     RAALAAANRNNDLDDDGLGLAEREWEEFWAATRPVELSAAVERHAGRLARLFVNTGAN
                     LTPFHRLKTDP"
BASE COUNT         1154 a         2889 c         2781 g         1166 t
ORIGIN      
        1 tggggcgcag caggaccgac agcaggagcg acgcttccgg cggcgccacc cacgaccggc
       61 ccatccggcc gcggcccgcg gtctggtgct cggccaccgc cacagccccc tcgggggcgc
      121 cggaccgggc ctggtcgagg acgtagcggt tcgtcgagtc gatagtgtcg aaccaccgca
      181 cgtcgccgaa ccgtgtcccg gccagccacc cgaccagctc gggaggcagc ccatcgccgt
      241 gggtggcgcc gggcacgggc atgccgtaac gatatggcgt ctcgaacgat atggcgtctc
      301 gatggaaagg aagtcgtgct caccaaggtt ctgatcgcca accgggccga gatcgccgtc
      361 cgggtcatcc ggacgtgccg ggagctgggc atcgcgaccg tcgcggtcta ctccgagctc
      421 gaccgggacg cgctgcacgt ccggctcgcc gacgaggcgt acgccctggg cggtcagacg
      481 gcggccgaga gctacctcaa cacgaccgcc atcatcgacg ccctgaaccg cagcggggcc
      541 gacggcgtgc accccgggta cggcttcttc gccgagaacg ccgacttcgc ccgcgccgtg
      601 caggcggcgg gcgtcacctg gatcggccct cctcccgcgg ccatcgaggt gatgggcgac
      661 aaggtcagct cccgcctggc cgccgcccgg gcggaggtag cggccgtgcc gggcaccacc
      721 gaggtcctca ccggtcccga ggacgtcgtg gccttcggca ccacccacgg gtggccggta
      781 gccataaagg cggcctacgg cggcggtggg cgggggatga aggtggtccc gtcggcagac
      841 gccgccgccg agctgctgga gtcggcccgc cgagaggcca aggccgcctt cggccgggac
      901 gagtgctacc tggagcgcta cctgacctgg ccccggcaca tcgagatgca gatcttcggc
      961 gacacccacg gcaactacgt ctggctcgga gagcgggact gctcgtcgca gcgccggcac
     1021 caaaagctga tcgaggagag cccggccccg ctgttccccg aggcggtccg ggtggccatg
     1081 ggcgaggcgg ccgtcaaggt ggcggcggcg tgcggctacc aaaacgcggg caccgtcgag
     1141 ttcatgttcc aggacggcga gttcttcttc ctggagatga acacccgcct ccaggtcgag
     1201 cacccggtca ccgagcaggt cgtcggccgc gacctggtcg agctgcagct gcggatcgcc
     1261 gcgggcgagc ccctgggctt cgaccaggcc gacatcgagc accacggcca tgccatcgag
     1321 tgccgcatca acgccgagga cccggcgggc ggccggttcg tgccctcgcc cggcaccatc
     1381 tcacggttcc gccggccgga cggttacggg gtgcggaccg acaccggcta cgacgagggc
     1441 gacacggtca gccagtacta cgacaacctg atcgccaagc tgatcgtctg gggacaggac
     1501 cgcgagcgcg cccgccggcg catgatccgg gccctcgagg agatggaggt cgagggcgtg
     1561 gcgaccacgg tgccggccca tctcgccata ctgcgccacc ctgacttcat cgagtccaag
     1621 cactcgacca ggtgggtcga ggacgtcctc gacctctcct cgctgaaccc tcctccggcg
     1681 ccggtccctc ccccggacga cgagccccgc gtgctgcgag aggtcgacgc cgaggtggac
     1741 ggaaggcgct acaaggtcag gctgtgggtg cccgacgtgg cacccgccct ggcggtcggc
     1801 ggcccggcgc ccgcccgggc cacccgctcc cgccccgccg ccgcggccgc ggcagccggc
     1861 tcgggcagcg gcaacgtcac cgtccccatg caggggacca tcgtcaaggt cctggtcgtg
     1921 gccggcgacg aggtcgaggc cggccaggcg gtctgcgtgc tcgaggccat gaagatggag
     1981 aacaacatca gcgccgagcg ctccggtcgc atcaccgagg tcaaggtcgt cccgggcgac
     2041 accgtgggcg ccggcgatat cgtcgccgtc atcgcctgat cgccaggcgc cgaggtgcca
     2101 agggggccga ccgtgcaagc cagcgaggcc aaggacgcag cccggcggtc cctcgggtcg
     2161 gcggccgacg cgctggtggc gttgagccac accatccacg cccatcccga gctggccttc
     2221 gaggaggaaa ggtcatcggg gtgggtcgca gacgccctgg ccggcgcggg cttcgcggtg
     2281 gagacagggg tcggcggcct gcccaccgcc ctggcggcca cggccgggtc gggccccctg
     2341 accctcgcca tctgcgccga gtacgacgcc ctgcccggaa tcggtcacgc gtgcgggcac
     2401 aacatcatcg cggccgcggc agtcggtgcg ggcctggcgc tggcgccgat cgcggatgac
     2461 ctcggcctga ccgtcaaggt gatgggaacc cctgccgagg agggcggcgg cggcaagatc
     2521 atcatgctcg aacggggcgt cttcgacggg gtcaacgcct cgatgatggt gcaccccgcg
     2581 ccgctcgagc aggcccagat gcactgcctg gcggtgtcgc acttcgacgt ccactacacg
     2641 ggcaaggagg cgcacgcgtc ggcattcccg gagcgcggcg tcaacgccgg cgatgccctc
     2701 accgtcgccc aggtggccct cgggctgctc cgccagcacg tcgacaccgg tgaccagttc
     2761 cacggcatcg tgaccaaggg aggtgaggct cccaacatcg tgcccgccca cacctcggcc
     2821 aagtactacg tgcgcgcctc gtcgttggag cgcctggccc acatcgagcc gcgcgtgcgc
     2881 cgctgcttcg aggccggcgc cctcgccacc ggcgccaccc tcgccattaa gccggccagc
     2941 ccgccctaca gcgagttcct cgccgaccaa cagctgctgg acacgtacgt gcggaacgcc
     3001 tcggcgctcg ggcgccgctt ctcggccacc gatggagggc tgcgcggcgg ctcgacggac
     3061 atggccaacc tttcactggc gatgccggcc atccatccca tgctcggcat cgagtcgctg
     3121 cccgccgtca accaccagcc cgagttcgcg gcagcgtgca tcaccccacc cgccgatcag
     3181 gcggtcaccg acggcgccct cgccatggcc tggacggcga tcgacctggc cctcgagccc
     3241 gagccgcggg ctcggctcct ggccttctcc gtcggggccg gcccgtagat ccagcatcag
     3301 tcgtaggccg cgaaaagctc ctcgagggcc ttgagctgcc cgcccgacgt ggacgacggc
     3361 acgaggatcg gagccttgac ccccgcctcg atccaggctt cgaccccttc ccgcacctgg
     3421 gccgcgctgc cgtacagagt gcagtccgac agccagcggt cggtcatcag ggccggcagc
     3481 cggtcgcggt cgcccgcggc cagcgccgcc tcgatggcct ccatctcctc gacgtagccc
     3541 gcggctttcc agtagttgcg gtagttgggc agcgccaggt agccggtgag cgacttgcgg
     3601 ttgacggccg ccgccgcctc ccggtcctcg tcgatcaccg tcgggatcat gttgccgacg
     3661 aagaactcgc cacccacctc cacagctgac agctgggcgg ccatgtggct ccgtgcggcg
     3721 ttcgcccaca cggccccgtc gctgatctcg gtcgccaggg cgagcatctt gtcccggagc
     3781 gtggccagca cgatcggagg caggggcccg acctgcttct cggcggcccg caggctgccc
     3841 acgtagtcgc gtgtgtcgcc cagcggcctg cccgtctcca cgcccatgcg ctgcatggcc
     3901 ggggcgtggc tcacgccgag ccccagccgg aagcgtccgc cgctcacctc gtgcaagtag
     3961 gaggcggccc ccgccagctc ctggggccgg cggaggtaga tcggctggat cgacgtcccg
     4021 aactccaggc gctccgtggc gtgcgccatc gacacgcaca ggctgatcgc atcgcccatg
     4081 atgctcgggc agtacacgcc ggtaaaccca cgatcctcga tctggcgagc cagctcgacg
     4141 gtgcggttcc gccgccccgg aacggcgaca agcgacacgg caggcattcg agacatggcc
     4201 gggaggctag ccaggggagc tactcggccg catccgcgag ggcctcggcc agcgccgggt
     4261 ggacgaggag gctctccacg atgtcggtga ccttgaggtt ggcggtgacg gccagggcga
     4321 tcaccgagat cagctcggcc gccctccggc ccacgatcga cccgcccagc accacgccgg
     4381 tcgccgggtc ggacaggatc ttcacgaagc cccggggatc gccgtcgatg agcgcccggg
     4441 cgttggccgc gaagggcacc ttggtcaccc ggatcttgcg gcccatggca aaggcctcgg
     4501 cctcggccag gccgacgtcc gcgatctcgg gcacggtgaa gatcgccgag gccgccttgt
     4561 cgtagtcgag gtggcggtgc tccctcgtgt gcagccccat cacgtgctcg gccaccttcc
     4621 ggccctgcat gaacgccacg ctcgccagcg ggagccggcc cgacacgtcc ccggcggcgt
     4681 agacgtgcgg gacgttcgtc tggcagtgct ggttgacggg cacgtagccg ccggcgtcca
     4741 cctccacgcc cgccgcctcc agccccaggc cctctgagtt gggcagcgag ccgatgcaca
     4801 gcagggcgtg cgagccgggc gcgacccgcc cgtcgtcgca ccggacggcg acgccaccgt
     4861 cctcggtgcg ctcgatggcc tccgcccggg cacccttgag gagccgcacg ccggtcgaga
     4921 tgaagtcgtt ctccagggcg gcggccacct cggggtcctt gttgggcagg acctgctggc
     4981 ggctcacgat cagggtgacg cgggcgccca gcgaccggaa catgtgggtg aactccacgc
     5041 ccgtcacccc cgagccgatg acgaccaggt gcgccggcag ctccttcggc gggtaggcgt
     5101 cgcgggtggt cagcacccgc tcgccgtcga tgtccgccca cggggggatg cgaggccggc
     5161 tcccggtgga cagcagcacg gcgtcggcgt cgagctcccg ggcaccttcc tcggtgtccg
     5221 ccaccagccg gtgcggcgac accagacggg ccgtgccccg caccacctcg acaccctggc
     5281 tggagagcag ctcggtgacc gaccgctcca gatgcctgct gacccccgcg atccggtcct
     5341 tcaaggcggc ggggtcgagg gtcggctgca gggcgcccag cccgagctgc tcggcccggc
     5401 gggacaggtt catgatcccg ccggccgcga tcatggcctt ggacgggatg cagtcccaca
     5461 ggtgggcagc gccgcccacg atgtcccgct cgatgaccgt gacgtcggcg ccgagacggg
     5521 cggcgtgggt ggcggcctgg acgcccgcgg gcccgccccc gatgatggcc aggctcacgg
     5581 gcatgggccg acgctacccg gcagcctgtc agcgggaggg ctggtacacc ccgaactcgc
     5641 cccgcaggac gtcgctcacc tcgccgagcg tggcccttcg gctcagggcc tctttcatcg
     5701 ggaccagcag gttctggctg cccctcgcgg ccgccgccac gtcggccagg gcggcgtcca
     5761 ccccggcctg gtcgcgctcc gcccggaccc gcctggtcct ctccacctgc cggcgggcca
     5821 gctccgggtc gatggggaac acgtccgcgt cctggacctc gtcggtggcg tactggttca
     5881 ccccgacgat gaccttctcg ccatcgtcca ccgccttcgt gtaggcatag gcggcctgct
     5941 cgatctcgtc ctgcatgtac ccggcctcga tggcggccac cgctcccccc atggcgtcga
     6001 tcttgtcgat gtaggcccac gcggcgtcct cgacctcgtc ggtcaacctt tcgacgaagt
     6061 acgagccccc cagtgggtcc accgtgtcgg ccacgcccga ctcgaagccg acgacctgct
     6121 gggtgcgcag cgccatcgtc gcggcccgct gggtcggcag gccgagggcc tcgtcgaagc
     6181 cgttggtgtg caggctctgg gtcccgccca tgacggccgc cagggcctgg agggtgacgc
     6241 gcacgatgtt gttctcaggc tgctgggcgg tgagcgtgga gccgccggtc tgggtgtgaa
     6301 agcgcagcag cttgctcttg gcgtccctgg cgccgaagcg gtcggtcatg atccgggccc
     6361 acatgcgccg ggcggcccgg tacttggcca cctcctcgaa gaagttgttg tgggcgttcc
     6421 agaagaagct cagcctcggg gcgaactcgt cgatggccag cccggtgtcc agcgcggcct
     6481 ggacgtaggc gatgccgttg gcgatggtga aggcgatctc ctgcaccgcc gtcgagccgg
     6541 cctcccggat gtggtagccg gagatcgaga tggtgttcca gctgggcagg ttctcgcggc
     6601 agtaggcgaa gatgtcggtg accagccgca tcgacggcct cggcgggtag atgtaggtcc
     6661 cccgggcgat gtactccttc agcacgtcgt tctggatggt gccacccacc cgggcggccg
     6721 gcaccccgtg ctcctccgcc accagctggt agagcaggag caggatggcc gccgtggcgt
     6781 tgatggtcat ggaggtcgtg acctggtcca ggggcaggtc ggccagcagc agccgcatgt
     6841 cctcgacgga gtcgatggcc acgcccacct tcccgatctc tccctcggcc cggggatggt
     6901 cggagtcgta gcccatctgg gtcgggaggt cgaacgcgca cgacaggccc gtggaccctg
     6961 ccgccgtcag ctgcttccaa cgctcgttgg tggcctcggc gttgccgaac ccgctgtact
     7021 gccgcatcgt ccacagccgg ccccggtaaa ggtccttgcg gatgccccgc gtgtagggat
     7081 agctgcccgg ctcccccagc ttggtggcgg ggtcccaccc ggccaggtcg ccggccgcgt
     7141 agacgggctg gatctcgatc cccgaatcgg tgcgccgtgg gtcgcgtgcc atagcagcag
     7201 gttacgccga gcaactggcg ggctcacagc ggtagctcga gccggccagg cggctcgctg
     7261 gtgttgcggg cgtcgcggaa tgcgctcaaa agtccgcacg tgcggactcg tccacgggac
     7321 gacgcggatc gggggcgggt cggcgacgta cactcagtgg tacgatgtgt tgtacacttg
     7381 tggcatggat gtagccgtga gcgagctgcg ggcgcacctg agtgactggc ttgaacgagc
     7441 cagcgagggc gaggaggtgg tcgtcacgga ccgcggggtc ccggtagcgc gcctcctcgg
     7501 catgacggcc accgcgacgc tcgaacgtct ggtcgccgac ggcgttattg cccgcccgga
     7561 gcgcgcccat cggccgcgtg catctggtcg cccgcgcccc cgtgtccgcc gtccggtcgc
     7621 cgatgtcata agcgatgaac gacgctgacg attgccactc gtctatttcg actccagcgc
     7681 tctggtgaag ctcctcgtgg aggaggccgg tagcgagctc gcggccgaga tctgggacgg
     7741 ctgtgacgcc gctattgcga gccgcctggc atatcccgaa gtccgagccg cgttggctgc
     7801 ggcgaaccgt aacaatgacc tcgacgatga tggcctcgga ctggccgaac gagaatggga
     7861 ggagttctgg gcagctactc gtcccgtcga gctcagcgcc gctgtggagc gccacgctgg
     7921 tcgattggcg cggttatttg tcaacaccgg cgcaaatttg acccccttcc accggttgaa
     7981 aactgacccc
//
LOCUS       DASWGG010000276         4705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3147354, whole genome shotgun sequence.
ACCESSION   DASWGG010000276 DASWGG010000000
VERSION     DASWGG010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3147354"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VF954_04935"
     CDS             complement(<1..425)
                     /locus_tag="VF954_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530461.1"
                     /translation="MIATVTDSGLRVYRSRRRQWLMASALVLALAVGWASILYPDPVT
                     HTVAGPGEYLWEVVPGVILVLVLAGRGLRSRLVTSRRGLLATRVTGRDWLPWSRVRRF
                     EVRKMAGGRAAAVVARLDDERVVRLWSYPAVRKEGGRSD"
     gene            complement(422..3487)
                     /locus_tag="VF954_04940"
     CDS             complement(422..3487)
                     /locus_tag="VF954_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEX9530462.1"
                     /translation="MKLDPVRDERIRQVLAAWVRPALCGPSAPLEVLASHVHGEPIGY
                     GEAVAGAFEPFPIGGAWGPPWGTTWFHLLGRVPPGWAGEEVVMRFEIGNAGDTGFGAE
                     ALVWCGDFPAQGLSPNHSRFRIAPRASGHEQVDLYVEAAANPRPPQHLTTWPLLLADP
                     HGAPAFTLARADLAVFRRQLFAFHQAFAALYGLYRDLPADEPRAAQLGAALDAACRAL
                     DLDDIPASYRQAKPILDDQLRPATASAHHMSAVGHAHIDTAWLWPLRETIRKCARTFS
                     TAVTLMDRYPEYRFACSQPQQYEWMKEHYPSLYRRMVEKVATGQLVPTGAMWVEADCN
                     VPSGESLVRQIVHGKRFFADELGVEVEEVWLPDVFGYAAGLPQIMRQGGVRWFLTQKL
                     SWNQYNRLPHHTFLWEGIDGSRVLTHFPPADTYNGAVTAEELRHAAGNFADHDRSGRS
                     LYLFGYGDGGGGPTEEMLETARAVADVEGLPRLTMDSPRAFFAAVEEELEDPPVWVGE
                     LYLEMHRGTYTTQAATKAGNRRGELALREAELWSALAARAPYPAAELDAAWKTLLLHQ
                     FHDIIPGSSIHWVYQDTERDHAAVIAFGERAARQALESVAAEASTEGMAEPVLLANSL
                     GWRRTGVIEGPAGPITVDLPACGYSVVDLADRPEPAASVHADPHGLENDAVRIAWDDQ
                     GSLSSIWDKGAGREVLAGPGNRFHLHPDHPNNYDAWDIDRFAFDTVTEIDGLVSMEVV
                     EDGPLRAAVRTVRRFSESTITQVISLSNAGLRIDFATEVDWHESHRLLKVAFPVAVRS
                     QRATYEVQFGHVERPTHANTSWDEARFEVCAHRWADLSEPGYGVALLNTAKYGYDIRG
                     HVMRLSLLRAPGWPDPQADRGRHRFTYSLLPHQGDLRDAGVIEAGYELNVAVQCVRVA
                     PGQAGPRPTSASFLSVDTPHVVLETVKQADDGDGLIVRGYEAWGRRGRARVACAADLR
                     GAEAVDLLERSLGPVAWDACGVDLEVRPFELFTLRLDVGPGPPGTLAPP"
     gene            3666..>4705
                     /locus_tag="VF954_04945"
     CDS             3666..>4705
                     /locus_tag="VF954_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530463.1"
                     /translation="MAAALTGAVVLLLPSTAFAARAQVARSRSAPRTAATPALTIGVD
                     NASPSGHNYLYTQFYPGSASTPTIHSGDVVDFQWNQGAIDGFHTATMLGSGENATPGD
                     PSSAWAKHPFFTPDADNGEAPLVNQQTDVAFGTFPPAGSGAPGACGDAATPCPFAADT
                     APSDLNSGAAPTAPGNDFFVKFTAAGPTTVTFVCLIHPGMKGHLTVDSGAAATTPAEA
                     SAAATTAYDADNTAAAAQIATVPAPTPTANGDGTKTWTLTAGTATPHVEILEMLPAGF
                     KIGKNDKVKWTTGTIEDVHTVTFPPGLGSISVDPFRLPPMCEAASGPDTVAPDLGGAP
                     PFGCADTASLEFP"
BASE COUNT          674 a         1724 c         1684 g          623 t
ORIGIN      
        1 ctgtccgagc gtccgccctc cttgcgcacg gccgggtagc tccacaaccg caccacccgc
       61 tcgtcgtcga ggcgggccac gacggccgcg gcccgcccac cggccatctt gcggacctcg
      121 aagcgccgga cgcgggacca cggcagccag tcgcgacccg tcacccgggt cgccagcagc
      181 ccgcgccgcg aagtcaccag ccggctccgc aggccccggc cggcgagaac gagcaccagg
      241 atgactccgg gcacaacctc ccacaggtac tcgcccggac cggcgacggt gtgggttaca
      301 gggtcgggat acaggatgga ggcccacccc acggccagcg caagcaccag ggccgacgcc
      361 atgagccact gccggcggcg gctgcggtat acccggagcc cggagtcggt gactgtcgcg
      421 atcatggcgg cgccagggta cccggcggcc cgggcccgac gtcgaggcgg agggtgaaca
      481 gctcgaacgg tcgcacctcc agatcgacgc cgcatgcgtc ccacgcgacc ggcccgaggg
      541 agcgctccag caggtccacc gcttcggcgc ccctgaggtc ggcggcgcag gcgacccggg
      601 cccggccccg ccggccccac gcctcgtagc cccgcacgat cagcccgtcg ccgtcgtcgg
      661 cctgcttcac cgtctcgagc accacgtggg gcgtgtccac cgacaggaag gacgccgacg
      721 tcgggcgagg tcccgcctgc ccgggcgcca cgcgcacgca ctgcaccgcc acgttgagct
      781 cgtagcccgc ttcgatcacg cccgcgtccc gcaggtcacc ctggtgcggc agcagcgagt
      841 aggtgaaccg gtggcggccc cggtcggcct ggggatcggg ccagcccgga gcacgaagca
      901 ggctgaggcg catgacgtgg ccgcggatgt cgtagccgta cttggccgtg ttgagcaggg
      961 cgaccccgta gccgggctcg gagaggtcgg cccagcggtg ggcgcacacc tcgaagcggg
     1021 cctcgtccca gctggtgttg gcgtgggtgg gccgctcgac gtggccgaac tgcacctcgt
     1081 aggtggcccg ctgcgagcgc accgctaccg ggaaggccac cttgagcagg cggtgcgact
     1141 cgtgccagtc gacctctgtg gcgaagtcga tgcgcaagcc ggcgttggag agcgagatca
     1201 cctgggtgat ggttgattcg gagaaacggc gcaccgtgcg cacggcagcc cggaggggcc
     1261 cgtcctcgac cacctccatc gacaccaggc cgtcgatctc tgtgacggtg tcgaaggcga
     1321 atcggtcgat gtcccacgcg tcgtagttgt tggggtggtc ggggtggagg tggaagcggt
     1381 tgccggggcc tgccagcacc tcgcgcccgg cgcccttgtc ccagatcgag ctgagcgagc
     1441 cctggtcgtc ccaggcgatg cggacggcgt cgttctccag gccgtggggg tcggcgtgga
     1501 cggacgccgc gggctccggc ctgtcggcaa ggtcgactac cgaatagccg cacgccggta
     1561 ggtcgacggt gatggggccg gccgggccct cgatcacgcc ggtgcggcgc cagccgagcg
     1621 agttggccag cagcaccggc tcggccatgc cctcggtgga tgcttcggcg gccaccgact
     1681 cgagggcctg ccgggccgcc ctttccccga aggcgatcac cgcggcgtgg tcccgctcgg
     1741 tgtcctggta cacccagtgg atgctcgagc ccgggatgat gtcatggaac tggtgcagaa
     1801 gcagggtctt ccaggccgcg tccagctcag cggccgggta cggcgccctc gccgccagcg
     1861 ccgaccacag ctccgcctcc cgcagcgcca gctcgccccg gcggttgccc gccttggtgg
     1921 cggcctgggt ggtgtaggtg ccgcggtgca tctcgaggta gagctcgccc acccacaccg
     1981 gcgggtcctc gagctcttcc tctacggcgg cgaagaacgc tcgcggcgag tccatcgtca
     2041 gccgcggcag cccctccacg tcggccaccg cccgcgccgt ctccagcatc tcctcggtcg
     2101 ggccgccgcc gccgtcgccg tagccgaaca ggtacagcga ccggccggag cggtcgtggt
     2161 cggcgaagtt ccccgccgcg tgccgcagct cctcggcggt gaccgcgccg ttgtaggtgt
     2221 ccgccggcgg gaagtgggtg agcacgcggg acccgtcgat cccctcccag aggaaggtgt
     2281 ggtgggggag ccggttgtac tggttccagg acagcttctg ggtcaggaac cagcgcacac
     2341 cgccctggcg catgatctgg ggcaggccgg cggcgtagcc gaacacgtcc ggcagccaga
     2401 cctcctccac ctcgacaccg agctcgtcgg cgaagaaccg tttgccgtgc acgatctggc
     2461 gcacgagcga ctcgcccgag ggcacgttgc agtcggcctc gacccacatg gccccggtcg
     2521 gcacgagctg gcctgtggcc accttctcca ccatgcgccg gtacaggctc gggtagtgct
     2581 ccttcatcca ctcgtactgc tggggctgcg agcaggcgaa gcggtactcg gggtagcggt
     2641 ccatcagcgt gacggccgtg gagaaggtcc gggcgcactt gcggatcgtc tcccgcaggg
     2701 gccatagcca cgcggtgtcg atgtgggcat ggccgaccgc gctcatgtgg tgggcgctcg
     2761 ctgtcgccgg ccggagctgg tcgtcgagga tgggcttggc ctggcggtag ctcgcgggga
     2821 tgtcatcgag gtcgagggcc cggcaggccg cgtccagggc cgcccccagc tgcgcggcgc
     2881 ggggctcgtc ggcgggcagg tcgcggtaca ggccgtagag ggccgcgaag gcctggtgga
     2941 aggcgaacag ctggcggcgg aacacggcca ggtcggcccg ggcgagggtg aaggcgggcg
     3001 cgccgtgcgg gtcggccagc agcaggggcc aggtcgtcag gtgctgcggc ggcctggggt
     3061 tggcggcggc ctcgacgtag aggtcgacct gctcgtggcc gctggccctg ggggcgatgc
     3121 ggaaccggct gtggttcggc gacagcccct gggccgggaa gtcgccgcac cacaccaggg
     3181 cctcggcgcc gaagccggtg tcgccggcgt tgccgatctc gaagcgcatg accacctcct
     3241 cgccggccca gcccggcggg acccgcccga ggaggtggaa ccacgtagtg ccccacggcg
     3301 ggccccacgc acccccgatc gggaagggct cgaaggcccc cgccacggcc tcgccgtagc
     3361 cgatgggctc gccgtggacg tggctcgcca gcacctcgag cggcgcgctc gggccgcaca
     3421 gcgccgggcg cacccacgcc gccagcacct ggcggatgcg ctcgtcgcgc accgggtcga
     3481 gcttcatcgg cccgaacggt agtgcggccc gggcccgggc ccgcctgccc gaaggtccgc
     3541 gccggctgcc cgaaggggca gtatccccac acggcaggtt cccagcgggt aagactctgc
     3601 gggccggcga cggatcggcg tcacaacagg gggttctcat gcggacatcg ggcaggcttc
     3661 gaatcgtggc agcggccctc accggcgcgg tggtgctgct cttacccagc acggctttcg
     3721 ccgctcgggc ccaggtcgcg agatcgaggt cggcgccgcg cacggcggcc actccggccc
     3781 tcacgatcgg ggtcgacaac gcctcgccca gcggccacaa ctacctgtac acgcagttct
     3841 accccgggtc cgcgtcgacc ccgacgatcc acagcggcga cgtggtcgac ttccagtgga
     3901 accagggtgc gatcgacggt ttccacaccg ccaccatgct cggatcgggc gagaacgcca
     3961 cgcccggcga ccccagcagc gcgtgggcca agcatccctt cttcacgcct gatgcggaca
     4021 acggcgaggc tcccctggtg aaccagcaga ccgacgtcgc cttcggcacc ttcccgcccg
     4081 ccggtagcgg cgcgcccggg gcgtgcggcg acgccgccac cccgtgcccg ttcgcggccg
     4141 acacggctcc aagcgacctc aactcggggg cggcgcccac cgccccgggc aacgacttct
     4201 tcgtcaagtt caccgcggcc gggcccacga cggtcacctt cgtgtgcctg atccacccgg
     4261 gcatgaaggg ccatctcacc gtcgacagcg gcgccgcggc caccaccccc gctgaggctt
     4321 cggccgcggc cacgaccgcc tacgacgccg acaacaccgc ggcggcggcc cagatcgcca
     4381 ccgtcccggc gccgacgccg acggccaacg gggacggcac caagacatgg acgttgacgg
     4441 cgggcaccgc caccccccac gtggagatcc tcgagatgct cccggccggc ttcaagatcg
     4501 gcaagaacga caaggtcaag tggaccaccg gcaccatcga ggacgttcac acggtgacgt
     4561 tcccgcctgg gctgggcagc atcagcgtcg atcccttccg cctgccaccg atgtgcgagg
     4621 cggcgtcggg tccggacacg gtcgcgcccg atctgggcgg ggcgccgcct ttcggctgtg
     4681 ccgacacggc atcgctggag tttcc
//
LOCUS       DASWGG010000277         4977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_894799, whole genome shotgun sequence.
ACCESSION   DASWGG010000277 DASWGG010000000
VERSION     DASWGG010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894799"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /gene="thrC"
                     /locus_tag="VF954_04950"
     CDS             <1..710
                     /gene="thrC"
                     /locus_tag="VF954_04950"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX9530464.1"
                     /translation="EGNYDDVNRLCAELASERPLWAFVNVNVRTYYAEGSKTLAFETA
                     EQLGWQAPDHVVVPVASGSQLTKIAKGFAELYQVGLLPGDGSHVRVSGAQAEGCSPVA
                     TAFATGADHIRPVKPSTIAKSLAIGNPADGWYALEAVRSSGGALASVTDAEILEGILL
                     LARTEGIFAETAGGVTIATLAKLAASGVVRADERVVAYVTGIGLKTIEAVAPIVSPTA
                     TIAPTLEAFNAALPVED"
     gene            726..1001
                     /locus_tag="VF954_04955"
     CDS             726..1001
                     /locus_tag="VF954_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX9530465.1"
                     /translation="MSVTVRIPTQLRPLTGGAGEVALDGSTVGEVLKALDAAHPGFGE
                     RLFDDGGRLRRFVNVFLADEDVRHLQGVDTPVAPGQTLSIIPAVAGG"
     gene            1047..1514
                     /locus_tag="VF954_04960"
     CDS             1047..1514
                     /locus_tag="VF954_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9530466.1"
                     /translation="MAEVLIVDDEPDIRGVLAFTLEDAGYAVREAANGAEAITALGAH
                     APDLVVLDLMMPGVDGFGVLRSRRQLGLAADARVIILTAKTAERDFVRGWELGADEYL
                     TKPFDPDVLLAKVRSLMACAPEELRSRREHELQKAELLERLESAFSRPRVSPR"
     gene            1638..3260
                     /gene="groL"
                     /locus_tag="VF954_04965"
     CDS             1638..3260
                     /gene="groL"
                     /locus_tag="VF954_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX9530467.1"
                     /translation="MPKMIAFDEQARRALESGMNQLADAVRVTLGPKGRNVVLEKKWG
                     APTITNDGVSIAKEIELEDPYERIGAELVKEVAKKTDDVAGDGTTTATVLAWAMVHEG
                     LRNVAAGANPMSLKRGIEAAVEAAIASLKDGAKEITDKEQIAQVGAISAADTEIGAMI
                     ADAIDKVGKDAVITVEESQTFGMEMDLVEGMRFDKGYISPYFVTDPERMESVLEDPYI
                     LYVGSKISAVRDLLPVLEKVMQGGKALAIVAEDVEGEALATLVVNKIRGTFKSAAIKA
                     PGFGERRKAMLQDMAILTGGQVITEEVGLKLENVTLDLLGRAAKVVVTKDETTIIDGA
                     GDENDIKGRINQIKNEIDNTDSDYDREKLQERLAKLSGGVAVIKVGAATEVELKEKKH
                     RIEDAVSTTKAAVEEGVVPGGGVALLRSQARILDVAEKLEGDEATGARIVARAVEEPL
                     KQIAVNAGLEGGVVIEKVKGLSGAAEGLNAATGEYEDLFKAGVIDAAKVTRSALQNAA
                     SIAALFLTTEAVVVDKPEKDAPPMPGGGMDDF"
     gene            complement(3318..>4977)
                     /locus_tag="VF954_04970"
     CDS             complement(3318..>4977)
                     /locus_tag="VF954_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX9530468.1"
                     /translation="FPIVAAAALLAGLSPAGAGAEVPILSISGKGFGHGVGMAQDGAY
                     WMGRQGATTPQILGQFYPGTAIGRAIGQVRVSLLATSTPDTDLVFPTGGQVRDSPAQA
                     QSPGFPVAVPARATAHVHFDGSRYSVTVSTASAVGPRGPRRGGGGGGQLAMGAAQAPL
                     PTLPSPSSTAPTSPATTAPVAAPTTTTAPPARPTTTSTAAPAPGSSTTTTTGVPAVSS
                     TRPLWAIGNGANGLVGIGGTPQRFRGVMETVADQSPAGAPVLRLVNQLDVEQYLRGMG
                     EVLAPAWPPASLRTQAIAARTYAMRAIAAGGEICPDTRCQVYLGQQVEYPAMDKAVAD
                     TAGQVLFYGGQLASTVYSANGGGYSASRAEGFGAEGATFAAAYPYLRAAPYPTNDPLP
                     WTVQVALTDVAARLAYHGTITGVTVSQAGPSGRALVVSLDGSAGRVGVTGLQMAAAFG
                     LRSTLFSTTVAATAAAPPPPPPPAAPAVQAPPDAAAAITAAAASPAAPPDPAPVTATA
                     KQAGTSGRHRGQAGLYLLGVLTVLNMGAGAAWTLWRRGLFDRLR"
BASE COUNT          764 a         1719 c         1861 g          633 t
ORIGIN      
        1 tcgagggcaa ctacgacgac gtcaaccggc tgtgcgccga gctggcgagc gagcgccccc
       61 tgtgggcgtt cgtcaacgtc aacgtgcgca cctactacgc cgagggctcc aagaccctgg
      121 cgttcgagac ggccgagcag ctgggctggc aggcccccga ccacgtggtc gtgccggtgg
      181 cgtcgggcag ccagctgacc aagatcgcca aggggttcgc cgagctgtac caggtcgggc
      241 tgctgcctgg cgacgggtcg cacgtgcgcg tgtccggcgc acaggccgag ggctgctccc
      301 ccgtcgccac cgcgttcgcg accggagccg accacatccg ccccgtaaag ccgtcgacca
      361 tcgccaagtc cctggcgatc ggaaacccgg cggacggctg gtacgcgctg gaggcggtgc
      421 ggtcgagcgg cggggcgctg gcctcggtga ccgacgccga gatcctggag ggcatcctgc
      481 tgctcgcccg gaccgagggc atcttcgccg agacggccgg tggtgtcacc atcgccaccc
      541 tggccaagct ggccgcgtcg ggggtggtgc gggccgacga gcgggtcgtg gcctacgtga
      601 ccggtatcgg gctcaagacg atcgaggcgg tggctccgat cgtgagcccc accgctacca
      661 tcgccccgac cctggaggcg ttcaatgccg cccttcccgt cgaagactga tcgaggagca
      721 tccgcatgag cgtgaccgtg cgcatcccga cccagctgcg ccccttgacc gggggcgcgg
      781 gagaggtcgc tcttgacggc tcgacggtgg gcgaggtgct caaggccctg gacgcggccc
      841 atcccggctt cggcgagcgg ctcttcgacg acggcggcag gctgcgccgg ttcgtgaacg
      901 tgttcctggc cgacgaggac gtgcgccatc tccagggcgt ggacacgccg gtcgcgccgg
      961 ggcagaccct ctcgatcatc ccggccgtcg ccggggggta agggccaggg ctcaaccggg
     1021 cgccccggcg cgccgatagg tgcgatatgg ctgaggtgct gatcgtcgac gacgagccag
     1081 acatccgggg agtcttggcc ttcacgctgg aggatgccgg gtacgccgtg cgcgaggccg
     1141 ccaacggcgc cgaagccatc accgccctgg gagcccacgc gcccgatctc gtggtgctcg
     1201 acctgatgat gcccggcgtc gacgggttcg gcgtgctgcg cagccggcgc cagctgggct
     1261 tggccgccga tgcccgggtg atcatcctca ccgccaagac cgcggagcgg gacttcgtgc
     1321 gggggtggga gctcggggcg gacgagtacc tgaccaagcc tttcgatccc gacgtcctgc
     1381 tggcgaaggt gcggtccctg atggcctgcg cccccgagga gcttcgcagc cgccgggagc
     1441 acgagctcca gaaggcggag ctgctggagc ggctcgagtc ggcgttcagc cggccccggg
     1501 tctccccccg ctagctcggc ctccccctcg cggcaggcgg cggcggccgg cgcttgcgaa
     1561 gcgttccgtc acgtggtttg ctggttagca ctcacagggt tcgagtgcta acggccaccc
     1621 acacggaggg aaaccaaatg ccgaagatga tcgcctttga cgagcaggcc cgccgggctc
     1681 tcgagagcgg catgaaccag ctggctgacg cggtacgggt cacgctgggc cccaagggcc
     1741 gcaacgtggt cctggagaag aagtggggcg cccccaccat caccaacgac ggggtgtcga
     1801 tcgccaagga gatcgagctg gaggacccgt acgagcggat cggcgccgag ctcgtcaagg
     1861 aggtcgccaa gaagaccgac gacgtcgcgg gcgacggcac caccaccgcc acggtgctgg
     1921 cctgggccat ggtgcacgag ggcctgcgca acgtggcggc cggcgccaac ccgatgtcgc
     1981 tcaagcgggg gatcgaggcc gcagtcgagg ccgccatcgc cagcctcaag gacggcgcca
     2041 aggagatcac cgacaaggag cagatcgccc aggtaggggc catctcggcg gccgacaccg
     2101 agatcggcgc catgatcgcc gacgccatcg acaaggtggg caaggacgcg gtcatcaccg
     2161 tcgaggagtc gcagaccttc ggcatggaga tggacctggt cgaggggatg cgcttcgaca
     2221 agggctacat ctccccctac ttcgtgaccg accccgagcg gatggagtcg gttctcgagg
     2281 acccctacat cctctacgtg ggctccaaga tctccgcggt gcgcgatctg ctcccggtgc
     2341 tcgagaaggt catgcagggc ggcaaggcgc tcgccatcgt cgccgaggac gtggagggcg
     2401 aggccctggc caccctggtg gtcaacaaga tccggggcac cttcaagagc gccgccatca
     2461 aggcccccgg cttcggcgag cggcgcaagg ccatgctcca ggacatggcc atcctgaccg
     2521 gcggccaggt catcaccgag gaggtcggcc tcaagctcga gaacgtcacc ctcgacctgc
     2581 tgggccgcgc cgccaaggtg gtcgtcacca aggacgagac gaccatcatc gacggcgccg
     2641 gcgacgagaa cgacatcaag ggccgcatca accagatcaa gaacgagatc gacaacaccg
     2701 actccgacta cgaccgggag aagctccagg agaggctggc caagctctcg ggcggcgtcg
     2761 ccgtcatcaa ggtcggggcc gccaccgagg tcgagctcaa ggagaagaag caccgcatcg
     2821 aggacgccgt gtcgaccacc aaggccgcgg tggaggaggg cgtggtgccc ggcggcggcg
     2881 tggccctgct acggagccag gcccgcatcc tcgacgtggc cgagaagctc gagggcgacg
     2941 aggccaccgg ggcacgcatc gtggcccggg cggtggagga gcccctcaag cagatcgccg
     3001 tcaacgcagg tctcgagggc ggcgtggtca tcgagaaggt caaggggctg tcgggtgcgg
     3061 ccgagggcct caacgccgcc accggcgagt acgaggacct gttcaaggcc ggcgtcatcg
     3121 acgccgccaa ggtgacccgc tcggccctcc agaacgcggc ctccatcgcc gcgctgttcc
     3181 tgaccaccga ggcggtcgtg gtcgacaagc ccgagaagga cgccccgccc atgccgggcg
     3241 ggggcatgga cgacttctag gccacatccg ccatcggtcg tgaggcgggg cgccccttcg
     3301 ggggcgcccc gccggcctta ccgcaaccgg tcgaacaggc cccgccgcca cagcgtccac
     3361 gccgcgcccg cacccatgtt gagcaccgtg agcacgccga ggagatagag cccggcctgg
     3421 ccccggtggc ggcccgaggt accggcctgc ttggccgtgg cggtgacggg cgcggggtct
     3481 ggcggggccg ccggtgaggc cgcggcggcg gtgatggcgg cggcggcgtc gggcggggcc
     3541 tgcaccgccg gggccgcggg cggaggcgga ggcggagggg ccgccgccgt ggctgccacc
     3601 gtggtcgaga agagcgtcga gcgcagcccg aaggccgcgg ccatctgaag accggtgacg
     3661 cccacccggc ctgcgctgcc gtccaggctc acgaccagcg ctcgacccga ggggccggct
     3721 tggctcaccg tcacgcccgt gatcgtgccg tgataggcca gccgggcggc tacgtcggtc
     3781 agggccacct gtacggtcca gggcaggggg tcgttggtcg ggtaaggggc ggcccggaga
     3841 taggggtagg cggccgcgaa ggtcgcgccc tccgccccga agccttcggc ccggctggcc
     3901 gagtacccgc caccgttggc cgagtacacc gtggatgcca gctgaccgcc gtagaagagg
     3961 acctgtccgg cggtgtcggc gacggccttg tccatggccg ggtactcgac ctgctggccg
     4021 aggtagacct ggcaccgggt gtcggggcag atctcgccgc cggccgcgat ggcccgcatg
     4081 gcgtaggtgc gggccgcgat cgcctgggtg cgaagtgagg ccggcggcca ggccggagcg
     4141 agcacctcgc ccatgccgcg caggtactgc tccacgtcga gctggttgac gagtcgcagc
     4201 accggcgccc ccgcgggcga ctggtcggcc accgtctcca tgaccccccg gaaccgctgc
     4261 ggcgtccccc cgatccccac caacccgttg gcgccgttgc caatggccca cagcggccgg
     4321 gtcgacgaca cggccggcac ccccgtggtc gtggtggtcg acgagccggg ggccggtgcc
     4381 gcggtggacg tggtcgtcgg gcgggccgga ggggccgtgg tcgtcgtggg cgcggcgacg
     4441 ggggcggtcg tcgccgggct ggtcggggcc gtgctggagg ggctcggcaa cgtggggagc
     4501 ggggcctggg cggcgcccat cgccagctgg ccgcctccgc cgcctccccg ccggggcccg
     4561 cggggaccca ccgccgatgc cgtcgagacg gtcacggaat accgggaccc gtcgaagtgg
     4621 acgtgggccg tcgcccgggc cgggacggcg acgggaaagc cgggggactg ggcctgcgcc
     4681 gggctgtcgc ggacctgccc gccagtcggg aacaccagat cggtgtcggg cgtcgacgtg
     4741 gcgaggagcg agacccgcac ctggccgatg gcgcggccga tggccgtgcc cgggtagaac
     4801 tggccgagga tctgcggcgt ggtcgctccc tggcgcccca tccagtaggc accgtcctgg
     4861 gccatgccca cgccgtgccc gaaccctttg ccggagatgc tcaggatcgg aacctcggcg
     4921 ccggccccgg cgggcgacag gccggcgagc aaggccgccg cggccacgat gggaaag
//
LOCUS       DASWGG010000278         4241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3154765, whole genome shotgun sequence.
ACCESSION   DASWGG010000278 DASWGG010000000
VERSION     DASWGG010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3154765"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VF954_04975"
     CDS             <1..842
                     /locus_tag="VF954_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9530469.1"
                     /translation="ALCEVGDEVVMFEPYYDSYGACVAMAGAGRRVVTLRPPDWSFDP
                     GALAAAITPRTRLVLLNTPHNPTGKVFGRDELECIASLCVERDLVAVTDEVYEHLVFE
                     GEHVPLATLPGMRERTLSISSAGKTFSVTGWKIGWVSGPEPLVDAVRTAKQFLTYVNG
                     APLQPAVAAGLGLADGMLRALADDLRAKRDLLSDGLATLGLEVFRPAATYFVMTDIRA
                     LGYDDGISFCRDLPGRCGVVAIPAVVFYDDKAAGRSLVRWAFCKQDEALHEALRRLDG
                     LHP"
     gene            942..1484
                     /locus_tag="VF954_04980"
     CDS             942..1484
                     /locus_tag="VF954_04980"
                     /inference="COORDINATES: protein motif:HMM:NF039480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6529 family protein"
                     /protein_id="HEX9530470.1"
                     /translation="MLRWRLVVALGVGSAVSIALGSYGRLHDPTRHALFTLFFTRTLN
                     MKAWLATLALALGGVQVVTALRMYGRLGWPRTLPPWWGDLHRLTGTLAFIVSLPVAFH
                     CLWSLGFKAHASQTRVFVHSVLGCAFYGAFAAKMVVVRSREAPDWALPVIGGAVFSVL
                     VGLWLTSSLWFFRHHGFPAV"
     gene            1487..1843
                     /locus_tag="VF954_04985"
     CDS             1487..1843
                     /locus_tag="VF954_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9530471.1"
                     /translation="MFRKLVDATEVLALAAAAAFVALMLFYRPTAARVGPVPGSGPSS
                     GAAIFAADCASCHGPRGEGELGPQLSGGAVMRAFPNEADQVALVTDGRGGMPAWAGRL
                     TPEQIKAVVDYTRSGL"
     gene            1980..2468
                     /locus_tag="VF954_04990"
     CDS             1980..2468
                     /locus_tag="VF954_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530472.1"
                     /translation="MDIEDFYDSDPRRRAVDEITFGLDWSDGEDAEHLGDLFWSSATG
                     ELYLMRKPHPRGLMGWDMYERRDVLRDVEDIGHHILGAAAHLLHPRQLRAKTGQAPRH
                     ARKDALTQELTVEVVAVVPDEAAARALLDGWEAEVDKPDSLGWLRARLAEHPAYAPPA
                     AG"
     gene            2499..3980
                     /locus_tag="VF954_04995"
     CDS             2499..3980
                     /locus_tag="VF954_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX9530473.1"
                     /translation="MTIVHRVLYPTPVESLEHYLEINGGVGWQLARKMDPADIIQVVA
                     DSGLRGRGGAGFPTGRKWRTVVDYEAGEMAKPSVVVNGAEGEPGTFKDRTILRNNPYQ
                     VVEGALIAARAVGADIVFFGLKHSFGREVARLRRAVDEIMAAGWDPAVELVVFEGPGE
                     YLYGEETALLESIDGRYPFPRIAPPFRRGVREIVETAGDVDAGSGLSAHVEMAGPSPE
                     TIAAPTLVNNVETMANVPRILARGAAWFRSEGTDESPGTVVCTVTGDTPRAGVGEVVM
                     GTPLRAAIEAIGGGARPGRTIRAVLSGVSNPVITAELLDTPLSYEAMAAAGTGLGSAG
                     FMVFDDSSDMAAVAAGVSRFLAVESCGQCSACKGDGLRISAALDRIVGDGPDPHDVAD
                     VDRRLAAVADGARCYLASQHQAVVGSLLTQFRSDLEGHLRGTAGPRRRALVAELKDVA
                     GEVAVVDPRFPSRQPDWTYDPEYSGQWPAARLDEHRRPEALED"
     gene            4015..>4241
                     /locus_tag="VF954_05000"
     CDS             4015..>4241
                     /locus_tag="VF954_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530474.1"
                     /translation="MAAVATAEGLAIVLLAVLVAGLLRSHAEILRRLHELGAGMDPAP
                     GAVPGRTSVALPATPPPDRRHPVPDLTGTTP"
BASE COUNT          539 a         1524 c         1561 g          617 t
ORIGIN      
        1 tggccctgtg cgaggtcggc gacgaggtgg tgatgttcga gccgtactac gactcgtacg
       61 gggcgtgcgt ggccatggcg ggggcggggc gacgcgtcgt gacgctccgc ccgcccgact
      121 ggtcgttcga tcccggcgcc ctggccgcgg ccatcacacc gcggacgcgc ctggtgcttc
      181 tcaacacccc gcacaacccg acgggcaagg tgtttggccg cgacgagctg gagtgcatcg
      241 ccagcctctg cgtcgagcgc gacctggtcg ccgtcaccga cgaggtctac gagcacctgg
      301 tgttcgaggg cgagcacgtc ccgctggcca ccctgcccgg gatgcgagag cggacgctga
      361 gcatctcctc ggcgggcaag accttctccg tcacgggctg gaagatcgga tgggtgagcg
      421 gtccggagcc gctggtcgac gcggtgcgca cggccaagca gttcctgaca tacgtgaacg
      481 gggcgccgct ccagcccgcc gtggccgccg ggctcggtct cgcggacggt atgctccggg
      541 ccttggccga cgacctgcgc gccaagcggg accttctttc cgatgggctg gccacgctgg
      601 gcctggaggt cttccggccg gcggccacgt acttcgtgat gaccgacatc cgggctctcg
      661 gctacgacga cggcatctcg ttctgccgcg acctgcccgg gcgctgcggc gtggtggcca
      721 tccctgccgt ggtcttctac gacgacaagg cggccgggcg ctcgctcgtg cgctgggcgt
      781 tctgcaagca ggacgaggcg ttgcacgagg ccctgcgccg gctcgacggc ttgcatcctt
      841 gaaccggccc ggccttgacc ggcgcccgcg cagcggcgag gctggcttgc cgtagcgact
      901 gccgaagggg gaccgtggcg accgaggcga cctcacaacc cgtgctccga tggcggctcg
      961 tggtcgcgct cggagtgggg tccgcggtat cgatcgccct cggctcctac ggccggctgc
     1021 acgatccgac gcgccacgcc ctcttcaccc tgttcttcac caggaccctg aacatgaaag
     1081 cctggctggc gaccctggcg ctggccctgg gcggggtcca ggtcgtcacc gccctgcgca
     1141 tgtacggccg gctgggttgg ccccgcaccc tcccgccgtg gtggggcgac ctccaccgcc
     1201 tcaccggcac cctcgccttc atcgtgagcc tgcccgtcgc cttccactgc ctgtggtcgc
     1261 tcggcttcaa ggcccacgcc agccagaccc gggtgttcgt gcactccgtg ctgggctgcg
     1321 ccttctacgg tgcgttcgcg gccaagatgg tcgtggtgcg gtcgcgggag gcgccggatt
     1381 gggcgctgcc cgtgatcggc ggcgcggtgt tcagcgtgct ggtcgggctg tggctgacga
     1441 gctccctgtg gttcttccgg caccacggat tcccggccgt gtgacgatgt tccgaaagct
     1501 cgtcgacgcc accgaggtgc tcgctctggc cgccgccgcc gccttcgtcg ccctcatgct
     1561 cttctaccgg ccgaccgccg cgagggtggg gcccgtgccg gggagcgggc cttcctcggg
     1621 cgcggcgatc ttcgcggccg actgcgcgtc gtgccacggc ccgcgcggtg aaggcgagct
     1681 gggcccccaa ctgtcgggcg gggccgtcat gcgggcgttt cccaacgagg cggaccaggt
     1741 cgccctcgtg accgacggca ggggcggcat gccggcgtgg gcgggccggc tcacgcctga
     1801 gcagatcaag gccgtggtgg actacacccg gtccgggttg tagccactgg ggtcgccggc
     1861 ccaccggggc gctacggtgg cgcgcatcac ccggtgggcc cggggagaac ggcccgtggg
     1921 gctgaccggg gcggagcatt ttcggcctgc gccccgggcc caccttctag gatcccgccg
     1981 tggacatcga agacttctac gactccgatc cccgccggcg agccgtggac gagatcacgt
     2041 tcgggctgga ctggagcgac ggggaggacg cggagcatct gggcgacctg ttctggagct
     2101 ccgcgaccgg tgagctgtac ctcatgcgca agccccaccc ccgcggcctg atgggctggg
     2161 acatgtacga gcgcagggac gtcctgcgcg acgtcgagga catcggccac cacatcctcg
     2221 gcgcggccgc ccacctgctg cacccccgcc agctccgggc caagaccggc caggcgcccc
     2281 gccacgcccg caaggacgcc ctcacccagg agctcaccgt cgaggtcgtc gccgtcgtgc
     2341 ccgacgaggc cgccgcccgg gcgctgctcg acggatggga ggccgaggtc gacaagcccg
     2401 acagcctggg ttggctgcgg gcccggctgg ccgagcaccc ggcctacgcc ccgcccgcgg
     2461 ccggctgacc ggcgcggcgg cctgaaagac tgcgagccat gaccatcgtt caccgtgtcc
     2521 tgtacccgac gccggtcgag tccctggagc actacctgga gatcaacggc ggggtgggat
     2581 ggcagctggc ccgcaagatg gacccggccg acatcatcca ggtcgtggcg gactcgggcc
     2641 tgcggggccg cggcggcgcc ggtttcccca ccggccgcaa gtggcggacc gtggtcgact
     2701 acgaggccgg cgagatggcc aagccctccg tcgtggtcaa cggcgccgag ggcgagccag
     2761 ggaccttcaa ggaccgcacg atcctgcgca acaaccccta ccaggtcgtc gagggggcgc
     2821 tcatcgcggc gcgtgcggtc ggtgccgaca tcgtgttctt cggcttgaag cacagcttcg
     2881 gccgggaggt cgcccgcttg cgcagggcgg tggacgagat catggcggcc ggttgggacc
     2941 cggccgtgga gctggtcgtg ttcgagggcc cgggcgagta cctctacggc gaggagaccg
     3001 cgctcctcga gtcgatcgac ggcaggtacc cctttcccag gatcgcccct ccgttccgcc
     3061 ggggtgtgcg cgagatcgtc gagaccgccg gcgacgtgga tgccgggtcc gggctctcgg
     3121 cgcacgtgga gatggccgga ccgagcccgg agaccatcgc cgcgcccacc ctggtcaaca
     3181 acgtcgagac gatggccaac gtgccgcgca tcctcgcccg gggcgcggcc tggttccgga
     3241 gcgagggcac cgacgagtcg cccgggacag tcgtctgcac cgtcaccggg gacacgccac
     3301 gcgccggcgt gggcgaggtc gtgatgggca cgccgctgcg cgccgcgatc gaggccatcg
     3361 gcggcggggc ccggccgggg cgcaccatcc gcgccgtgct ctcgggggtg tcaaaccccg
     3421 tgatcaccgc ggagctgctc gacacgccgc tgtcctacga ggccatggcc gcggccggga
     3481 cggggctggg gtccgccggg ttcatggtgt tcgacgactc ctcggacatg gcggcggtcg
     3541 cggccggggt ctcgcggttc ctggccgtgg agtcctgcgg ccagtgctcg gcctgcaagg
     3601 gggacggcct gcgcatctcg gccgccctcg accgcatcgt cggggacggt cccgacccgc
     3661 acgacgtggc cgacgtcgac cggcggctgg ccgccgtggc cgacggggcc cggtgctacc
     3721 tggccagcca gcaccaggcc gtggtcggca gcctgctcac gcagttccgg tcggacctgg
     3781 agggccacct ccgcgggacc gcaggcccgc gccgccgtgc cctagtcgcc gagctgaagg
     3841 acgtggccgg cgaagtggcg gtcgtggacc cccggttccc gtcgaggcag ccggactgga
     3901 cctacgaccc cgagtactcg gggcagtggc cggcggcgcg cctcgacgag caccggcggc
     3961 ccgaggccct cgaggactga gccaggcccg cgccagaacg atagtgtcgg acgggtggct
     4021 gcggtagcaa cggccgaagg gctggcgatc gtgctgctcg cggtgcttgt ggccgggctg
     4081 ctgcgcagcc acgccgagat ccttcgccgc ctgcacgagc tgggcgcggg gatggaccct
     4141 gcgccggggg cggtgccggg ccggacgtcg gtcgcgttgc cggcaacccc cccaccggat
     4201 cgtcgccatc cggtgccgga cctcacaggg accacgcccc a
//
LOCUS       DASWGG010000279         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3154971, whole genome shotgun sequence.
ACCESSION   DASWGG010000279 DASWGG010000000
VERSION     DASWGG010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3154971"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1105
                     /locus_tag="VF954_05005"
     CDS             146..1105
                     /locus_tag="VF954_05005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9530475.1"
                     /translation="MVRHGVTIPFDGAPLADQREMITELVDLGYTDVWSSEANGTDAF
                     IPLALASAWSPTLRLGCAIVPAYTRGPALMAQTVAAMEEAAPGRFAFGIGTSSNVIVE
                     NWNGIPFEEPYKRTRDMVRFLKAALTGAKIDEKYDTFTVKGFRLGRKPPSVPPILVAA
                     LRPGMLHLAAREAEGAIINWLSAEDVTKVAAEVGPGKEIVARIFVCPSEDADAVRSAG
                     RFAIAAYLNVPVYAAFHEWLGRGPQLAAMWDAWKAGDRKAATAAIPDEVVDALIVHGS
                     PASCRAHIQRYIDNGVTTTALAIMAFGIDSRQAVRDLAPAATT"
     gene            1181..>2185
                     /locus_tag="VF954_05010"
     CDS             1181..>2185
                     /locus_tag="VF954_05010"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9530476.1"
                     /translation="MRIRGNRAAGAERAVASPIHLELIGDRQALDLVRRLARAAPDIH
                     HVTRLLASHVTALGESGGVWLLDPASRRLRPAAIVGPGLGDAELGDQLQRALGTGRAV
                     HLGDQILGMGIELRGRMAGAVAVARPPGGPSFGEAEAAALRDAALQAALAIDNSRLYA
                     SSTAETAERRRVEGDLRSHAVQQAALAELSQRALGGADPADLLRDTVIVVAQTLDVPV
                     TALVEAKGDQLVLRAGVGWDAPGAPTGHAQGAAAVERDEPVIVNDGSESAAAVPVPGR
                     IHPFGALEVYGRARAFVPEDVSFLQAVANVVALAVERAADEAEILHQALHDSLTGLPN
                     R"
BASE COUNT          303 a          824 c          767 g          291 t
ORIGIN      
        1 cgggccggcg caagaaggag tcgaccaaag ccgctccggc aaccgacccg tccaccacgc
       61 aacgtagtca gaaagtcgcc tgaaacccct tgacatcagt tagggactca gccactgccg
      121 cggcccgggc cgtagagtcc cagtcatggt gcgtcacggt gtcacgatcc ccttcgacgg
      181 agcacccctc gccgaccagc gggagatgat caccgagctg gtcgacctgg ggtacaccga
      241 cgtgtggtcc agcgaggcca acgggaccga cgccttcatc cccctcgccc tggcctcggc
      301 gtggtccccg accttgcgcc tgggctgcgc catcgtgccg gcctacacgc ggggcccggc
      361 gctcatggcc cagaccgtgg cggccatgga ggaggccgcc cccggccggt tcgcgttcgg
      421 catcggaacg tcctccaacg tcatcgtcga gaactggaac ggcatcccct tcgaggagcc
      481 ctacaagagg acccgcgaca tggtgcggtt cctcaaggcc gcgctcacgg gggcgaagat
      541 cgacgagaag tacgacacct tcaccgtcaa gggcttccgc ctcggtcgca agcccccttc
      601 ggttccgccc atcctcgtgg ccgccctgcg gccgggcatg ctgcacctgg ccgcacggga
      661 ggcggagggc gccatcatca actggctctc ggccgaagac gtgaccaagg tggcggccga
      721 ggtcgggccg ggcaaggaga tcgtggcccg gatcttcgtg tgcccgtccg aggacgccga
      781 cgctgtccgg tccgcggggc gcttcgccat cgcggcctac ctcaacgtcc ccgtctacgc
      841 cgccttccac gagtggctgg ggcggggccc gcagctggcc gccatgtggg acgcgtggaa
      901 ggccggcgac cgcaaggcgg ccaccgcggc cattcccgac gaggtggtcg acgccctgat
      961 cgtgcacggc tcgccggcct cgtgccgggc gcacatccag cgctacatcg acaacggggt
     1021 caccaccacg gccctggcca tcatggcctt cgggatcgac agccgccagg ccgtccggga
     1081 cctggccccc gccgccacca cctgagcatc cacccgcggc cccgcgccgg ctgggtacgg
     1141 tgggtgggca cctgaccact gatcgtcaga gaggttctgc atgcgcatca ggggaaacag
     1201 ggcggccggt gccgaacggg cggtcgcgtc ccccatccat ttggagctca tcggggaccg
     1261 ccaagccctc gacctggtcc gccgcctggc gcgggccgcg cccgacatcc accacgtcac
     1321 ccgcctcctg gcctctcacg tgaccgccct gggcgagagc ggcggcgttt ggctgctcga
     1381 cccggcgtcc cggcggctcc ggccggctgc gatcgtgggc ccgggtctgg gcgacgccga
     1441 gctcggcgac cagctccaac gtgccctcgg caccggccgc gccgtgcacc tcggcgacca
     1501 gattctcggc atgggcatcg agctgcgggg ccggatggcg ggcgcggtgg ccgtggcccg
     1561 gccgccgggc ggtccgtcgt tcggggaggc ggaggcggcg gccctgcgtg acgccgccct
     1621 ccaggctgcc ctggccatcg acaactcccg cctgtacgcg tcctcgacgg ccgagacggc
     1681 cgagcgccgc cgggtcgagg gcgacctgcg cagccacgcg gtgcagcagg cggcgctcgc
     1741 cgagctgtcg cagcgggccc tgggcggcgc cgaccccgcc gacctgctca gggacacggt
     1801 tatcgtcgtg gcccagaccc tcgacgtccc ggtcaccgct ctggtcgagg ccaagggaga
     1861 ccagttggtg ctgcgcgccg gggtcgggtg ggacgccccc ggcgccccca ccggccatgc
     1921 ccagggcgcg gcggctgtcg agcgcgacga gcccgtgatc gtgaacgacg ggtcggagag
     1981 cgccgccgcc gtgcccgtcc cgggccggat ccatcctttc ggagcgctgg aggtgtacgg
     2041 ccgggcccgc gccttcgtgc ccgaggacgt gagcttcctc caggccgtgg ccaacgtggt
     2101 ggccctcgcg gtcgagcggg ccgcggacga ggcagagatc ctgcaccagg cgctccacga
     2161 ctcgctgacc gggctgccca accgg
//
LOCUS       DASWGG010000280         7813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2376376, whole genome shotgun sequence.
ACCESSION   DASWGG010000280 DASWGG010000000
VERSION     DASWGG010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2376376"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VF954_05015"
     CDS             <1..864
                     /locus_tag="VF954_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530477.1"
                     /translation="EVLSEGQMAPVPGIGLDDLLKTDPDSQDDHLAAFPRIMVLQPPA
                     IDPFAPADGQQVTVAWSESNDGADSGGHLSTVGWWLSDHWADPIQEVICDPMTHGQTV
                     QHSITMNGVAAGDWMIVVTANADGAQDTTAPPTATGFGTMVSTPVRVGGGNASDRPAS
                     PGDANWTGYGDALRFLSQAYSAEGNDNALSAAAQALYSFAGSLDSGGVADPETRKPGD
                     AGSIACVQRAQALEAKVGTIEWDREVTTAYQTELGAAIEALSAITDPSEGGAAAVAAM
                     LGIGLDSLGLR"
     gene            complement(927..1011)
                     /locus_tag="VF954_05020"
     tRNA            complement(927..1011)
                     /locus_tag="VF954_05020"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(975..977),aa:Tyr,seq:gta)
     gene            1039..1527
                     /locus_tag="VF954_05025"
     CDS             1039..1527
                     /locus_tag="VF954_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX9530478.1"
                     /translation="MPTFDVVSEVDMQEVRNAVDQASREVGTRFDFKDTGSSIELSGQ
                     DMHLTSSTEDRLRALTQVLEEKLVKRSVSLKALDRGKVEEAARSTVRQTIGLQAGISA
                     ENARRLNKFIKEHAPKGVSSQTQGEQLRVSGKKRDDLQAVIAALKAEDFGIPLQFSNF
                     RE"
     gene            1524..2036
                     /locus_tag="VF954_05030"
     CDS             1524..2036
                     /locus_tag="VF954_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530479.1"
                     /translation="MRSRRGGTWSGVAVVLLGAACSARGINGEASDDRAAGELRAAAG
                     VSLGASFRFRGTEAKGGTDLPDVNGTFSPTSRVRLDRPAPGALPAVADPALLLSDIEG
                     TRYVTRSSGSLRFNLVATDLDALVGGGAADDDDFAGEAFVSRGKVARVRFQATRAGVH
                     YLGDVAISPA"
     gene            complement(2165..3028)
                     /locus_tag="VF954_05035"
     CDS             complement(2165..3028)
                     /locus_tag="VF954_05035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX9530480.1"
                     /translation="MSKNTIKTYVLLAALGGLMIVIGGAIGGGNGALIGLLIGVAFSG
                     GSYWFSDTLAIKAARAVPVSEAEMPEYYQVVRELTQRAGMPMPRLYMTPDMQPNAFAT
                     GRNPEHAAVAVTQGILQTLDWEELRGVLAHEISHVGNRDILLGSVAAAIAMGITFIAR
                     MALWGGALFGGGGGGRDRRDNILADLALLILAPIAATMLQMALTRSREYEADRSGARL
                     IGDGEPLARALMKIEAGVKAVPMQVMPAEASMFIVNPLTGRQVQFKKLWMTHPPTADR
                     IERLRSGEWRR"
     gene            complement(3075..3581)
                     /locus_tag="VF954_05040"
     CDS             complement(3075..3581)
                     /locus_tag="VF954_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530481.1"
                     /translation="MTAPPRRGAEPDNWRWRAGPVEAGPAVVFDIDGVLSDAASRQHY
                     IESGRRAWDSFFDACGEDPLIEEVARLLELLDPSLRVVLLTGRPIRVQPQTLAWLRRY
                     ELRWDVLIMRESGDYSRAWEFKRWTVAELRAYGFALRLAFEDDRRNLEMFRDEGVPCV
                     YIHSGYFD"
     gene            complement(3578..5551)
                     /gene="acs"
                     /locus_tag="VF954_05045"
     CDS             complement(3578..5551)
                     /gene="acs"
                     /locus_tag="VF954_05045"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX9530482.1"
                     /translation="MSDASPAAIEALQLESRTFPPSEATRRQALVSDAGIYQEAEADW
                     QGFWARQAGELDWYRPWDTVLEWDLPFAKWFVGGKLNVSYNCLDRHVAAGRGDRIAYN
                     WEGEPGDARSITYSDLLDEVCRFANVLKDLGVARGDRVAIYLPMIPELPVAMLACARI
                     GAPHSVVFGGFSSDALRDRINDAEAKVVVTADGGWRRGSIVPLKANVDAALAETPSIR
                     HAVVVRRTGAPGGGEVPMAEGRDVWWHDLTPAASMECPPEEMDSEDLLYLLYTSGTTA
                     KPKGIMHTTGGYLTQVSFTHKYVFDLHADTDVYWCAADIGWVTGHSYIVYGPLANGAT
                     SLMYEGTPDFPDKDRLWSMVERYGVTILYTAPTAIRTFMKWGPELPARHDMSSLRVLG
                     SVGEPINPEAWVWYWQHIGGGRCPVVDTWWQTETGSIMISPLPGATVLKPGSATFPLP
                     GISAEVVDDQGKPVERGGGYLTIDRPWPAMLRGIYGDPERYRDTYWSRFEGRYFAGDG
                     ARVDEDGYFWLMGRVDDVMNISGHRVSTTEVESALVDHPTVAEAAVVGAKDDITGQAI
                     IAFVTVKAGETSDEVHGQALRQHVAAKIGAIARPKTIIFTDELPKTRSGKIMRRLLRD
                     VAEGRDLGDTTTLADAAVVEEIRRRASTSPTEE"
     gene            complement(5621..6829)
                     /locus_tag="VF954_05050"
     CDS             complement(5621..6829)
                     /locus_tag="VF954_05050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9530483.1"
                     /translation="MPPDGAGSLLLRLNERIRRQGPLPFSAFMEACLYDERDGFYTSG
                     RGAGRQRDFLTSPEVGPLFGEVVARALDERWARLGRPDPFVVIECGAGAGTLARDVLA
                     AGPLCGPALRYLLVERSAALRDAQAAVVALELPTFVLGPTNAEDDPEGERVGERGRGP
                     LAAGLAELPADPVTGVVLANELLDNLAFDLLQRAEPGWEEVRVGVEPAGPTRLIEPAG
                     PTRLIEVPVPAAGHLSALADALAPDAPVGGRIPVQAEARRWLLAALGALSRGAVIAFD
                     YADSTASLGSRPWTEWVRTYRGHGRGGRPLDDPGGQDVTCEVAVDQLAAVAVPTSVRR
                     QAEWLADHGLAELADRARAEWQERAAVGDLAAVRARSRLGEAAALADESGLGAFTVLE
                     WEVGSRGGAR"
     gene            complement(6837..>7813)
                     /locus_tag="VF954_05055"
     CDS             complement(6837..>7813)
                     /locus_tag="VF954_05055"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX9530484.1"
                     /translation="ATGVMLYGMSLIIGVTGTSVLSQISAAIGTTAHSTPVVSIGIVF
                     ILVGFSFKISAVPFHFWAPDTYEGSPTPVTAFLSVASKTGGFVALMELVYAGFFPRHD
                     VWMPLLWGLAALTMTVGNLVALRQTNIVRMLAYSSIAQAGYILVPFAVAASNATTDHV
                     RSVQTASIVYLLIYSAMNLGAFAVVIAVARKTRSGEISSYGGLIDHAPGLTVVMTVFL
                     FSLAGIPPFGGWFAKFYVFRAVIDASTPAATVLGVIVAVNSVIALFYYAGVARQMWFS
                     DVPDEDRSPIRVPPPLAAALGLTAAMTVAIGIYPNLFARMGDVSSLVH"
BASE COUNT         1175 a         2690 c         2878 g         1070 t
ORIGIN      
        1 gaggtgctct ctgagggcca gatggcgccg gtgccgggca tcgggctcga cgacctgctg
       61 aagacggacc cggacagcca ggacgaccac ttggccgcct ttcccaggat catggtcctc
      121 cagcctcctg cgatagatcc cttcgccccg gcggacggcc agcaggtcac ggtggcctgg
      181 agcgagagca acgacggcgc cgactcgggc ggccatctgt cgaccgtcgg ctggtggctc
      241 agcgaccact gggccgaccc gatccaggag gtcatctgcg atcccatgac ccacgggcag
      301 acggtgcagc actccatcac gatgaacggc gtcgcggcgg gcgactggat gatcgtcgtg
      361 acagccaacg ccgatggcgc ccaggacacg accgctccgc ccacggccac gggcttcggg
      421 acgatggtct ccacccccgt tcgagtcggt ggcggcaacg cgagcgaccg gccggcgagc
      481 ccgggggatg ccaactggac gggctacggc gacgccctgc gcttcctgag ccaggcctac
      541 tcggcggagg gcaacgacaa cgccctgagc gcggcggccc aggccctcta ctccttcgcc
      601 ggcagcctcg actccggtgg agtagccgat cccgagacac gcaaaccggg tgacgccggc
      661 tccatcgcgt gcgtgcaacg agcccaggcg ctcgaggcaa aggtcggcac gatcgagtgg
      721 gaccgcgagg tgaccaccgc ctatcagacc gagctgggcg cggccatcga ggccctgtcg
      781 gccatcaccg acccgagcga aggcggggcg gccgcggtgg ccgcgatgct gggcataggc
      841 ctcgattcgc tcgggttgcg ctgagccgtc acttggaaga gcgcggcatc tactccgtct
      901 gatcagccaa ccacggcgga cggaaatggt gggccgggaa ggatttgaac ctccgtaggc
      961 tgtgccggca gatttacagt ctgctccctt tggccgctcg ggcaccgacc caacggggca
     1021 ggaggatagc gtttcgccat gcccaccttc gatgtggtct cagaggtcga catgcaagag
     1081 gtccgcaacg ccgtggacca ggcgtcgcgc gaggtgggta cccgcttcga cttcaaggac
     1141 acgggctcgt ccatcgagct gagcggccag gatatgcacc tcacctcctc gaccgaggac
     1201 cgcctccgcg ccctgaccca ggtgctcgag gagaagctgg tgaagcggtc ggtgtcgctc
     1261 aaggccctgg accggggcaa ggtcgaggag gccgcccgga gcacggtgcg ccagacgatc
     1321 gggctccagg ccgggatctc cgcggagaac gccaggcggc tcaacaagtt catcaaggag
     1381 cacgccccga aaggggtcag ctcccagacc cagggtgagc agctgcgcgt gtccggcaag
     1441 aagcgcgacg atctccaggc cgtgatagcc gccctgaagg cagaggactt cgggatcccg
     1501 ctccagttca gcaacttccg ggagtgagga gccgccgggg gggcacgtgg tcgggtgtcg
     1561 ccgtggtgct tctcggggcc gcgtgcagcg cgcgcggcat caacggggag gcgagcgacg
     1621 accgggcggc cggtgagctg cgggcggctg cgggggtgtc gctgggggcg tcgttccggt
     1681 tccggggcac cgaggccaag ggcggcaccg acctgcccga cgtcaacggg accttttccc
     1741 ccacctctcg cgtgcgcctc gatcggcccg cgccgggtgc gctgcccgcg gtcgccgacc
     1801 cggccttgct cctctccgac atcgaaggca ccaggtacgt gacccgctcg tcgggatcgc
     1861 tgcgcttcaa cctggtggcc accgacctgg acgccctggt gggaggcggg gccgccgacg
     1921 acgacgactt cgccggggaa gccttcgtct cccggggcaa ggtcgcccga gtccgcttcc
     1981 aggcgacccg ggccggcgtg cactacctgg gcgacgtggc gatctcgccg gcgtagcccg
     2041 ggaggcgcgg cagcgctgtc ctcgcgccga accgggcgtt gctggggaga ttaggtggtc
     2101 tgagaccgcc tcatctcccc agtaaaatga aatgctatga aattcggcct cggggcggtg
     2161 gcccctaccg gcgccactcc cccgagcgga gccgctcgat ccggtcggcg gtcggcgggt
     2221 gggtcatcca cagtttcttg aactggacct gccgaccggt cagcgggttg acgatgaaca
     2281 tcgacgcctc ggccggcatg acctgcatgg ggacggcctt gacgcccgcc tctatcttca
     2341 tgagggcccg ggcgaggggc tccccgtcgc cgatgaggcg ggcgccggag cggtcggcct
     2401 cgtactcccg gctgcgggtc agcgccatct ggagcatggt ggcggcgatc ggcgccagga
     2461 tgagcagcgc caggtcggcg aggatgttgt cgcgccggtc ccggccgccg cccccgccgc
     2521 cgaagagggc gccgccccac agcgccatcc gggcgatgaa ggtgatgccc atggcgatgg
     2581 ccgccgcgac cgacccgagc aggatgtccc ggttgcccac gtggctgatc tcgtgcgcca
     2641 gcacgccgcg cagctcctcc cagtcgaggg tctgcaggat cccctgggtc accgccacgg
     2701 cggcgtgctc cgggttgcgg ccggtggcga aggcgttggg ctgcatgtcc ggcgtcatgt
     2761 agaggcgggg catcggcatc ccggcccgct gggtcagctc ccgtaccacc tgatagtact
     2821 ccggcatctc ggcctcgctc accggcaccg cccgggcggc cttgatggcc agggtgtcgg
     2881 agaaccagta ggagccgccg ctgaaggcca cgccgatgag caggccgatg agcgcaccgt
     2941 tgccgccacc gatggcgcca ccgatgacga tcatcagccc gcccagggct gccagaagca
     3001 cgtaggtctt gatggtgttc ttgctcatgt caccttcggt aacgccggtc cggctggcaa
     3061 tagttccggg gcggtcagtc gaagtagccg gagtggatgt agacgcacgg gacgccctcg
     3121 tcgcggaaca tctccaggtt acggcgatcg tcctcgaacg ccagccgcag ggcgaagccg
     3181 taggccctca gctccgcgac cgtccaacgt ttgaactccc aggcccgcga gtagtcgccg
     3241 gactcgcgca tgatgagcac gtcccaccgc agctcgtagc gccggagcca ggcgagggtc
     3301 tggggctgga cccgtatcgg ccgcccggtg agcagcacca cccgcagcga cgggtcgagc
     3361 agctcgagca gccgggccac ctcctcgatc agcgggtcct cgccgcacgc gtcgaagaac
     3421 gagtcccacg cccgccggcc cgactcgatg tagtgctggc ggctggccgc gtcggacagg
     3481 acaccgtcga tgtcgaagac gaccgccggc cccgcctcga ccggtccggc tcgccagcgc
     3541 cagttgtcgg gctcggcgcc acggcggggc ggcgcggtca ctcctcggtc gggctggtcg
     3601 acgcccggcg gcggatctcc tccaccacgg cggcgtcggc gagggtggtg gtgtcaccga
     3661 ggtcgcggcc ctcggccacg tcgcgcagca gccgccgcat gatcttgccg ctgcgggtct
     3721 tcggcagctc gtcggtgaag atgatggtct tgggccgggc gatggcgccg atcttggcgg
     3781 ccacgtgctg gcggagggcc tggccgtgga cctcgtcgga ggtctccccc gccttcaccg
     3841 tgacgaaggc gatgatggcc tggccggtga tgtcgtcctt ggccccgacc acggcggcct
     3901 cggcgaccgt cgggtggtcg accagggcgg actcgacctc ggtggtggag acgcggtggc
     3961 ccgagatgtt catcacgtcg tcgacgcggc ccatgagcca gaagtagccg tcctcgtcga
     4021 cgcgggcgcc gtcgccggcg aagtaccggc cctcgaagcg gctccagtac gtgtcccgat
     4081 agcgctcggg atcgccgtag atcccccgaa gcatggcggg ccagggccgg tcgatggtga
     4141 ggtagccccc gccccgctcg accggcttgc cctggtcgtc gacgacctca gccgagatgc
     4201 cgggcagggg gaaggtggcc gagccgggct tgagaaccgt ggcaccgggc agcggcgaga
     4261 tcatgatcga gcccgtctcc gtctgccacc acgtgtccac caccgggcag cggccgccgc
     4321 cgatgtgctg ccagtaccag acccaggcct cggggttgat gggctcgccc accgagccga
     4381 ggacgcgcag gctggacatg tcgtggcggg cgggaagctc cgggccccat ttcatgaacg
     4441 tgcggatcgc ggtcggggcc gtgtagagga tggtgacccc gtagcgctcg accatggacc
     4501 acaggcggtc cttgtcgggg aaatcgggag tcccctcgta catgaggctg gtggccccgt
     4561 tcgccagggg cccgtagacg atgtagctgt gaccggtgac ccagccgatg tcggccgcgc
     4621 accagtacac gtcggtgtct gcatgcaggt cgaacacgta cttgtgggtg aagctcacct
     4681 gcgtgagata gccgccggtg gtgtgcatga tgcccttggg cttggccgtg gtgcctgagg
     4741 tgtacagcag gtagagcagg tcctcgctgt ccatctcctc gggcgggcac tccatggacg
     4801 cggccggcgt gaggtcgtgc caccagacgt cgcggccctc ggccatgggc acctcgccgc
     4861 ccccgggggc gccggtgcgg cggaccacca cggcgtggcg gatcgacggg gtctcggcca
     4921 gggcggcatc cacgttggcc ttgaggggga cgatcgagcc tcgccgccac ccgccgtcgg
     4981 cggtgacgac gaccttggcc tcggcgtcgt tgatgcggtc gcgcagggcg tcggaggaga
     5041 agccgccgaa gaccacggag tggggcgccc cgatccgggc gcaggcgagc atggccacgg
     5101 gcagctcggg gatcatcggc aggtagatgg cgacccggtc ccccctggcc acgccgaggt
     5161 ccttgaggac gttggcgaag cggcagacct cgtcgagcag gtcggagtag gtgatgctgc
     5221 gggcgtcgcc cggctcgcct tcccagttgt aggcgatccg gtcgccccgc ccggccgcca
     5281 cgtgccggtc caggcagttg tacgagacgt tgagcttgcc gcccacgaac cacttggcaa
     5341 agggcaggtc ccactccagg accgtgtccc acgggcggta ccagtccagc tcgcccgcct
     5401 gccgggccca gaagccctgc cagtcggcct cggcctcctg gtagatgccg gcgtcgctga
     5461 ccagggcctg gcgcctggtg gcctcggacg gcgggaaggt ccggctctcc agctggaggg
     5521 cctcgatggc ggccgggctc gcatcggaca tgccgcggag cctagcggcg gggggtgccg
     5581 gcgctcacct ggccgccggc gctcatccgg ccgccggcgc tcacctggcc ccgccgcggg
     5641 agcccacctc ccactcgagg acggtgaacg ctcccagccc cgactcgtcg gccagggcgg
     5701 cggcctctcc cagccggctg cgggcccgaa cggcggccag gtcgccgacc gcggcccgtt
     5761 cctgccactc ggcccgggcc cggtcggcca gctcggccag cccgtggtcc gccagccact
     5821 cggcctggcg gcgcaccgag gtgggaacgg ccactgcggc gagctggtcg accgccacct
     5881 cgcaggtcac gtcctgcccc ccggggtcgt cgaggggccg cccgccccgg ccgtggccgc
     5941 ggtaggtgcg cacccactcc gtccagggcc gggaccccag cgaggccgtc gaatcggcgt
     6001 agtcgaaggc gatcaccgcg ccccggctca gcgccccgag ggcggcgagc agccaccgcc
     6061 gggcctcggc ctgcaccggg atgcgtccgc ccaccggggc gtccggagcc agggcgtcgg
     6121 ccagggccga gaggtgcccg gccgccggca ccggcacctc gatcaggcgg gtcgggccgg
     6181 cgggctcgat caggcgggtc gggccggcgg gctcgacccc cacccgcacc tcctcccagc
     6241 cgggctcggc ccgctggagg aggtcgaagg ccaggttgtc gagcagctcg ttggcgagca
     6301 cgacgccggt caccgggtcg gccggcagct cggccagccc ggccgccagc gggccccggc
     6361 cccgttcgcc gacccgctcg ccctcgggat cgtcctcggc gttggtgggc ccgagcacga
     6421 aggtgggcag ctccagggcc accacggccg cctgggcgtc gcggagggcg gcgctgcgct
     6481 ccacgagcag gtaccgcagg gccgggccgc acagggggcc ggcggctagg acgtcgcggg
     6541 cgagggtgcc ggcgccagcg ccgcactcta tgaccacgaa ggggtcgggc cggcccagcc
     6601 gggcccaccg ctcgtcgagg gcgcgggcca ccacctcgcc gaagaggggg ccgacctcgg
     6661 ggctggtcag gaagtcgcgc tgccggccgg cgccacggcc cgaggtgtag aagccgtccc
     6721 gctcgtcgta caggcaggcc tccatgaacg ccgagaaggg cagggggcct tgacggcgga
     6781 tgcgctcgtt gaggcgcagg aggagggagc ccgcgccgtc aggcggcatg cccgtgtcag
     6841 tgcaccagcg acgagacgtc gcccatgcgg gcgaagaggt tgggatagat gcctatggcg
     6901 accgtcatcg ccgcggtgag gcccagggcg gctgcgaggg gcgggggcac ccggatgggg
     6961 ctgcgatcct cgtcggggac gtcgctgaac cacatctggc gggccacgcc ggcgtaatag
     7021 aacagagcga tcaccgagtt gacggccacg atcacaccga ggacggtggc ggccggcgtg
     7081 ctggcgtcga tcacggcccg gaagacgtag aacttggcga accagccgcc gaagggcggg
     7141 atcccggcca gggagaagag gaacaccgtc atcacgaccg tgagcccggg ggcgtggtcg
     7201 atgagaccgc cgtaggagga gatctcgccg gaacgggtct tgcgggccac ggcgatgacg
     7261 acggcgaagg cgcccaggtt catggccgag tagatgagga ggtacacgat gctggccgtc
     7321 tgcacgctgc gcacgtggtc ggtggtggcg ttggacgcgg ccaccgcgaa gggcaccagg
     7381 atgtagccgg cctgggcgat cgacgagtag gcgagcatgc gcacgatgtt ggtctggcgc
     7441 agggcgacca ggttgccgac ggtcatggtg agcgccgcca gcccccacaa cagcggcatc
     7501 cacacgtcgt ggcgcgggaa gaagccggcg tagacgagct ccatgagggc cacgaagccg
     7561 cccgtcttgg acgctacgga caggaaggcg gtgaccggcg tgggcgatcc ctcgtaggtg
     7621 tcgggcgccc agaagtggaa gggcaccgcc gatatcttga aagagaagcc gaccaggatg
     7681 aaaacgatgc cgatgctcac cacgggcgtg gagtgcgcgg tggtgccgat ggcggcggag
     7741 atctggctca gcaccgaggt ccctgtcacc ccgatgatga gcgacatccc gtagagcatc
     7801 acgccggtcg cca
//
LOCUS       DASWGG010000281         8719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1545998, whole genome shotgun sequence.
ACCESSION   DASWGG010000281 DASWGG010000000
VERSION     DASWGG010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545998"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VF954_05060"
     CDS             complement(<1..212)
                     /locus_tag="VF954_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX9530485.1"
                     /translation="MAPSAGNSLHVPASDLETVLVEVARGGDRQLLREVESLTEALST
                     RTIIANAVGLLMASGAVTRDEAFDQLR"
     gene            278..1324
                     /gene="selD"
                     /locus_tag="VF954_05065"
     CDS             278..1324
                     /gene="selD"
                     /locus_tag="VF954_05065"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:335..337,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX9530486.1"
                     /translation="MSDHDRSSRRLTEYSHGAGUACKLAPGELAQVLRHLTPTPHPDL
                     LVGTATGDDAAVWRIDQGRALVVTTDFITPVVDDARTWGRIAAANAVSDIYAMGGRPF
                     LALNLVAWNRDELPLELLGEVLAGGADIAAGSGFVIAGGHTVDDPEPKYGLAVVGEAD
                     PDRLLTNAALRAGDRLILTKPLGVGLAATALKRGDAAPALVDAATRSMVRTNAAAAEV
                     ALASGATGATDVTGFGLLGHLGRMLEESGLDAEVRVERVPLLAGVLALAEAGVVPGGT
                     RRNLEWARHRLDPGGVAEPMQLVLADAQTSGGLLFGVAPQEAGRALHALEDSGHQAAD
                     IGEVAAGAGRIALL"
     gene            1366..3504
                     /gene="ppk1"
                     /locus_tag="VF954_05070"
     CDS             1366..3504
                     /gene="ppk1"
                     /locus_tag="VF954_05070"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX9530487.1"
                     /translation="MSSLATTWQPPAAPPEPLLPIGADDELRFLNRELSRLDFHSRLL
                     DLAHDGSVRMLERAKFLAIFSSNLDEFFQVQVANMQDLVAGGLGAPSPDGRSPAAQLH
                     EIRSKVEQLIACQGGIFREELLADLAGAGIRLSEWGSLDDGDRSHLVDVFEHRIFPVL
                     TPLSVDPAHPFPEISNLSLNLAVMVVNPHSGERRFARVKVPPLLPRFVVMPDGERFVP
                     LEQVIAAHLPALFRGMEIESQFPFRVTRNADVMVEEEETDDLLEAMEIELRRRRFGKA
                     VRLEVDARMTDEVRDLLLRELELEPEDLYAIEGPLDLSGLWAVYDLDRPDLKDDPWAP
                     MTQPSLATPDGEPLELFGVLRERDVLVHHPYDSFTTSVEELIKQAAADPHVLAIKQTL
                     YRTGGDSEIVQALIAAAVAGKQVVVLVELKARFDEERNIGWARQLEDAGVHVVYGVVG
                     LKTHAKIALVVREEDDGIRRYCHVGTGNYNSRTARQYSDLGLLSADPAFGADLTELFN
                     FLTGYSRSVDYERILVAPTSLRNDIIELIQAEARAGSDGRIVIKANHLVDPATIDALY
                     DASRAGVDIDLVIRSICCLRPGVPGLSDHIRVRSIVGRYLEHSRIFSFGGRSGRALRM
                     YIGSADLWQRNLDRRIEVVAPVHDPQLQAGLWEVLDVNLRDDTNAWELQPDGSWRRTE
                     MTEGTSTFRQLQDAAMERARRRRDPESLAR"
     gene            3512..5014
                     /locus_tag="VF954_05075"
     CDS             3512..5014
                     /locus_tag="VF954_05075"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HEX9530488.1"
                     /translation="MGVEREVKLAADGAFELPDLAGVVEGAAAVALPEEKLDASYHDT
                     ADLRLARWGVTVRFRREGRAGAWWTVKLPDDATLRDGLARDELAFPGGPGAMPPDALR
                     LLTAYVRGASLAPVARLVTRRRRTQLVDAAGAAIAEVDDDVVAVMDGRHVVARFREIE
                     IEALAPGGDRVLGPVRDALVRAGARPTVPVPKVIRALGARAADAPEVAAVEVGHWATA
                     GQVVRAAIGSGVTRMLRHDPGVRLGEDPEAVHQARVGTRRLRSDLRTFRRLLEAEWVG
                     PTREALGWVAGALGAVRDDDVLLERLRGQVLVLPAADRNAARALLRRLETQRALHREE
                     LLAVLDSPRYTAFLDRLVGAAAAPPLTGAAAAPALEVLPDLVRRPWRHLARAVDRLPE
                     DPEPEALHRVRVRAKRSRYAAESAEAVMGKPARRLAAAVADVQAVLGDLNDAVVAEAW
                     LRQAAHGSAAQALVAGQLVALQREEVAAARAAWPAAWAAASRKKLRSWLT"
     gene            5040..5510
                     /locus_tag="VF954_05080"
     CDS             5040..5510
                     /locus_tag="VF954_05080"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX9530489.1"
                     /translation="MAVLLVRHAKAGHRDRWAGDDRLRPLTPRGKDQAEALADLLVPY
                     QPALLLSSPFVRCVQTVEPLAAALGVPVEEDGALAEGQAAAAVQLAARLAGSSVAVCS
                     HGDVIPAMLDALARAAGIRLPPGPACAKGSVWVVEGSGTRAAMARYLPAPSRSS"
     gene            5565..7133
                     /locus_tag="VF954_05085"
     CDS             5565..7133
                     /locus_tag="VF954_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX9530490.1"
                     /translation="MRLAALDMGSNSFHLLVVQVREDGGFLPLTREKEMLRLGDVVTR
                     HGYVTPEAEDRAVAAVRRLCGLADAAGAEELVVCATSAIREAANSGEVVDRIREETGV
                     RARVIGGLEEARLIFRAVQASILIDPGPALALDLGGGSLEVMVGDARALHWARSAKLG
                     VARLTAELVRHDPASTGDRKRLEKRVDEVLEPLAGEVAAFAPRMVVGTSGTFCTLARM
                     IEARRSGTLPTSVNQLTVTRAEIDALADELASSSVAARRRLPGLDERRADLILAGAVI
                     AQGALRTFAGEGLTVGDWALREGMVLDAIDRHDPTEFSGDPRAIRRASVLSLCRRCGW
                     DEVHSSQVARLALQLFDGTAGLHRLGGADRELLEHGALLHDIGEHVSPEGHHKHTAYL
                     IEHGRLRGFEPAEVAVLATLGRYHLRGDPKAVFEPFGRLDTDRQRQVRVLVGLLRVAD
                     GLDRSHAATVGDLDVMAGRPSIRIVVGAHGDPELEVWGARRKLDLLERALGRPVMVDA
                     AHPGTLARQRLRGA"
     gene            7200..8066
                     /gene="pstB"
                     /locus_tag="VF954_05090"
     CDS             7200..8066
                     /gene="pstB"
                     /locus_tag="VF954_05090"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX9530491.1"
                     /translation="MVDSKLKAPVRIAAPAPSREVGRAGREPTGEPAFSARDVRFFYG
                     SHLALQGVTLDIPTRQVTALIGPSGCGKSTFLRCLNRMNDEIRGARMEGSVTYRGVDL
                     YADGVEPTAVRRTIGMVFQRPNPFPKSIYDNVAFGVRALGLRKGDLEDAVEQALRRVA
                     LWDEVARKLKASALGLSGGQQQRLCIARALAVDPDVILMDEPCSALDPIATLKIEQLI
                     GELRLDYTIVIVTHNLQQAARVSDRTAFFTVEATGPGARTGLLVEVGPTAQIFGSSKD
                     QRTDDYVAGRFG"
     gene            complement(8071..>8719)
                     /locus_tag="VF954_05095"
     CDS             complement(8071..>8719)
                     /locus_tag="VF954_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEX9530492.1"
                     /translation="RTGETITTLAGAGDAVLAAAVGETEPDAALADVLATDARVDALL
                     EWLEGEVFDLVARQSPVGRDLRFLTASLRIGQALERVGDLLVSTGRRVEVVRPLVAGH
                     GLEGPLRRLGDGALDLLRRAGAAYAVFDAEAASQLAADDDAVDAAHGELRRQLFSLGE
                     VPAEPVVELSMVGRFFERVADHAVVIGERVCFIASGVMHPGDADESTIWAHPDDP"
BASE COUNT         1141 a         3224 c         3157 g         1197 t
ORIGIN      
        1 cgcagctggt cgaaagcctc gtcgcgggtc accgcgcccg aggccatcag caggccgacg
       61 gcgttggcga tgatggtgcg ggtgctgagc gcctcggtga ggctctccac ctcccggagc
      121 agctggcggt cgccgccgcg ggccacctcc accagcaccg tttcgaggtc gctcgcgggg
      181 acgtgcaggg agttgccggc gctcggtgcc acggcgctca gtgtacggca tgggcgccgg
      241 tacagtcggc ccaccgcccg ccgaagggag ggacgacatg agcgatcacg accggtcgag
      301 ccgacggctc accgagtaca gccacggagc gggctgagcc tgcaagctcg cccccggcga
      361 gctggcgcag gtgctgcgcc atctgacacc gacgccgcac cccgacctgc tggtggggac
      421 ggcgacagga gacgatgcgg ccgtctggcg catcgaccaa ggtcgcgccc tcgtggtcac
      481 caccgacttc atcacccccg tcgtggacga cgcgcgaacg tggggccgga tcgcggccgc
      541 caacgccgtg tcggacatct acgccatggg agggcgaccg ttcctcgccc tcaacctggt
      601 ggcgtggaac cgcgacgagc tgccgctcga gctgctcggc gaggtgctcg cgggcggtgc
      661 cgacatcgcc gcggggtcgg gctttgtgat cgccgggggg cacacggtcg acgaccccga
      721 gcccaagtac ggcctcgccg tggtaggaga ggccgacccg gaccgccttc tcaccaacgc
      781 cgccctccgc gccggcgacc gcctcatcct caccaagccg ctcggcgtgg ggctggcggc
      841 cacggcgctc aagcgaggcg acgccgcccc cgcgctggtc gacgccgcca cgcgctcgat
      901 ggtgcgcaca aacgctgcgg ccgccgaggt ggctctggct tccggcgcga ccggtgcgac
      961 cgacgtcacc gggttcggtc tactcggcca cctcggccgg atgctcgagg agtcgggtct
     1021 ggatgccgag gtccgcgtgg agcgggtgcc gctccttgcg ggggtgctgg cgctggccga
     1081 ggcgggtgtc gtaccgggtg gcacgaggcg caacctggag tgggcccggc accgcctgga
     1141 cccgggcggg gtggccgagc ccatgcagct ggtgctggcc gacgcccaga cctccggcgg
     1201 tttgctcttt ggggtggccc cgcaggaggc gggccgggcc ctgcacgcgc tggaggacag
     1261 cgggcatcag gcggcagaca tcggggaggt ggccgccggg gccgggcgga tagctctgct
     1321 gtgaggacgt aagtgacgga taagaggacg taggtgacgg ataaggtgag ctccttggct
     1381 actacctggc agccacccgc cgcccccccc gagcccctcc tgccgatcgg cgccgacgac
     1441 gagctgcggt tcctcaaccg tgagctctcc cgcctcgatt tccactcgag gctgctcgac
     1501 ctggcccacg acggctccgt caggatgctc gagcgggcca agttcctggc catcttctcg
     1561 tccaacctgg acgagttctt ccaggtccag gtggccaaca tgcaggacct ggtggccggc
     1621 gggctgggcg ccccctcccc cgacgggcgg tcgcccgcgg ctcagctcca cgagatccgg
     1681 tccaaggtcg agcagctcat cgcgtgccag ggcggcatct tccgggagga gctcctggcg
     1741 gacctggccg gagccggcat ccggctctcc gaatgggggt ccctggacga cggggaccgc
     1801 tcccacctgg tggacgtctt cgagcaccgc atcttcccag tgctcacgcc gctctccgtg
     1861 gacccggctc accctttccc cgagatctcc aacctctcgc tcaatctcgc cgtgatggtg
     1921 gtcaatcccc acagcggcga gcgccgcttc gcccgggtca aggtgccacc gctgctgccg
     1981 cggttcgtgg tcatgcccga cggcgagcgc ttcgtgccgc tcgagcaggt catcgcagcc
     2041 catctcccgg cgctgttccg gggcatggag atcgagtcgc agttcccgtt ccgggtcacg
     2101 cgcaacgccg acgtgatggt ggaggaggag gagaccgacg acctgctcga ggccatggag
     2161 atcgagctgc gccggcggcg gttcggcaag gccgtccggc tggaggtcga cgcccggatg
     2221 accgacgagg tccgggacct gctgctccgg gagctggagc tcgagcccga ggacctctac
     2281 gccatcgagg ggcccctcga cctgtcgggg ttgtgggccg tctacgacct ggaccgcccg
     2341 gacctcaagg acgatccctg ggccccgatg acccagccga gcctggccac ccccgacggg
     2401 gagcccctgg agctgttcgg cgtcctccgg gagcgtgacg tcctcgtcca tcatccctac
     2461 gactcgttca ccacctcggt cgaggagctc atcaagcagg ccgcggccga cccgcacgtc
     2521 ctggccatca agcagacgct ctaccgcacg ggcggcgaca gcgagatcgt ccaggccctg
     2581 atcgccgcag ccgtggcggg caagcaggtc gtggtcctgg tcgagctcaa ggcccgcttc
     2641 gacgaggagc gcaacatcgg ctgggcccgc cagctcgagg acgccggggt ccacgtggtc
     2701 tacggggtcg tgggcctgaa gacccacgcc aagatcgcgc tcgtcgtgcg ggaggaggac
     2761 gacggcatcc gtcgctactg ccacgtcggc accggcaact acaactcccg cacggcccgc
     2821 cagtactccg acctcggtct gctgtccgcc gacccggcgt tcggcgccga cctgacggag
     2881 ctgttcaact tcctgaccgg ctacagccgc agcgtcgact acgagcgcat cctggtggcc
     2941 ccgacctcgc tgcgcaacga catcatcgag ctgatccagg ccgaggcccg ggccggctcc
     3001 gacggccgga tcgtgatcaa ggccaaccac ctggtcgacc cggccaccat cgatgccctc
     3061 tacgacgcgt cccgcgccgg ggtcgacatc gacctcgtca tccgctccat ctgctgcctg
     3121 cggcccggcg tgccggggct ctcggaccac atccgtgtcc gcagcatcgt gggccgctac
     3181 ctcgagcact cgcggatctt cagcttcggg ggccggtccg ggcgggccct gcgcatgtac
     3241 ataggctcgg ccgacctctg gcagcgcaac ctcgaccggc ggatcgaggt ggtggcgccg
     3301 gtccacgacc cccagctcca ggcgggcctg tgggaggtcc tcgacgtcaa cctgcgcgac
     3361 gacaccaacg cctgggagct ccagcccgac gggtcgtggc gccggacgga gatgaccgag
     3421 ggcacgagca ccttccggca gctccaggac gcggccatgg agcgggcccg ccgccgccgc
     3481 gatcccgagt cgctcgcccg ctgacgcacc ggtgggcgtc gagcgcgagg tgaagctggc
     3541 cgccgacggt gccttcgagc tccccgacct ggcgggcgtg gtcgaagggg ccgctgccgt
     3601 ggcccttccc gaggagaagc tcgacgcctc ctaccacgac accgccgacc tccgcctggc
     3661 ccggtggggg gtcaccgtgc gcttccggcg ggaggggagg gcgggggcgt ggtggacggt
     3721 gaagcttccc gacgatgcca cgctccgcga tgggctggcc cgcgacgagc tggcctttcc
     3781 cggtggcccg ggcgccatgc ctcccgacgc cctccggctg ttgacggcgt acgtcagggg
     3841 cgcctcgctc gctccggtcg cccggctggt cacccggcgg cggcgtaccc agcttgtgga
     3901 cgccgccggc gcggccatcg ccgaggtgga cgacgacgtg gtggcggtga tggacggccg
     3961 ccacgtggtc gcccggttcc gggagatcga gatcgaggcg ctcgctccgg gcggcgatcg
     4021 tgtcctgggt ccggtgcgcg acgccctggt gcgcgccggc gcccgcccga ccgtgccggt
     4081 ccccaaggtc atccgagctc taggtgctcg cgccgccgac gcgccggagg tcgcggccgt
     4141 ggaggtgggg cactgggcga ccgccggcca ggtcgtgcgg gccgccatcg ggtccggcgt
     4201 gacccgcatg ctgcgccacg acccgggggt gcgcctgggt gaggacccgg aggccgtcca
     4261 ccaggcccgg gtgggcacgc gccggctgcg ctcggacctt cggacgtttc gccgccttct
     4321 ggaggccgag tgggtggggc cgacccgtga ggcgctggga tgggtggcgg gtgccctggg
     4381 ggccgtccgc gacgacgacg tgctcctgga gcggctccgg ggccaggtgc tggtactgcc
     4441 ggcggcggac cgcaacgcgg cgcgcgccct gctgcgacgg ctcgagacgc agagagccct
     4501 ccatcgcgag gagctgctgg cggtcctcga cagcccgcgg tacacagcgt tcctcgaccg
     4561 gctggtgggc gccgccgcgg ccccgccgct gaccggcgcg gccgccgccc ccgccctcga
     4621 ggtgctgccg gacctggttc gccgcccgtg gcgccacctc gcccgggccg tggaccgcct
     4681 gcccgaggat cctgagcccg aggccctgca ccgggtgagg gtccgggcca agcgcagccg
     4741 ctatgccgcc gagtcggccg aggcggtcat gggcaagcct gcccgccgac tggcggccgc
     4801 ggtcgccgac gtgcaggcgg tcctcggcga cctcaacgac gccgtggtag ccgaggcctg
     4861 gctgcgccaa gccgcccacg gctcggccgc ccaggccctg gtcgcgggcc agctcgtcgc
     4921 cctccagcgg gaggaggtgg ccgccgcccg ggcggcgtgg ccggcggcgt gggcggcggc
     4981 cagccgcaag aagctgcgct cctggctgac ctgagcacag cgggccacaa tggcagctca
     5041 tggccgtgct cctcgtccgc cacgccaagg cgggccaccg cgaccgctgg gccggtgacg
     5101 accgcctccg gccgttgacg ccgcggggaa aggatcaggc cgaggccctg gccgacctcc
     5161 tggtcccgta ccagccggcc ctgctgctct ccagcccgtt cgttcgctgc gtgcagacgg
     5221 tggagccgct ggcggcggcc ctcggggtgc ccgtcgagga ggacggggcc ctggccgagg
     5281 ggcaggcggc ggcggccgtc cagctggccg ctcggctggc cgggtcctcg gtcgcggtct
     5341 gcagccacgg cgacgtgatc cccgccatgc tggacgccct ggcccgggcc gccggcatcc
     5401 gcctgccgcc gggaccggcg tgcgccaaag ggtcggtgtg ggtggtcgag gggtccggga
     5461 cgcgggctgc gatggctcgg tacctcccgg cgccgtcccg atcgtcctga accggcaggg
     5521 ggctccgcgg tacccagtcg gctttcccct acggtaggag caccatgcgc ctggccgccc
     5581 tcgacatggg cagcaactcg ttccatctcc tcgtcgtcca agtccgtgag gatggtggtt
     5641 tcctcccgct gacccgggag aaggagatgc tccggctcgg cgacgtggtc acccggcacg
     5701 gctacgtcac ccccgaggca gaggaccggg cggtggcggc agtgcgccgg ctctgcggcc
     5761 tggccgacgc ggcgggtgcg gaggagctgg tggtgtgcgc gacgagcgcc atacgggagg
     5821 ccgccaactc tggcgaggtg gtcgacagga tcagggagga gaccggcgta cgggcccgag
     5881 tcatcggcgg gctggaggag gcccgcctca tcttccgcgc cgtgcaggcg agcatcctga
     5941 tcgaccccgg cccggccctg gccctggacc tgggcggcgg gagcctcgag gtgatggtgg
     6001 gcgacgcccg ggccctccac tgggcgcgca gcgccaagct gggcgtggcc cggctcacgg
     6061 ccgagctggt ccgccacgac cccgcttcga caggggaccg caagcggctg gagaagcggg
     6121 tcgacgaggt gctcgaaccc ctcgccggcg aggtggccgc cttcgcgccc cggatggtgg
     6181 tgggcaccag cgggacgttc tgcacgctcg cccggatgat cgaggccagg cggtccggca
     6241 ctctgcccac gtcggtcaac cagctgaccg tgacgcgggc cgagatcgac gccctcgccg
     6301 acgagctggc ctcctcgtcg gtcgccgccc gccgccgcct gccggggctc gacgagcgcc
     6361 gcgccgatct catcctggcc ggcgcggtga tcgcccaggg cgctctgcgc accttcgccg
     6421 gcgaagggct gacggtgggg gactgggccc tgcgcgaagg catggtgctg gatgcgatcg
     6481 accgccacga cccgaccgag ttctcgggcg acccccgggc catccggcgg gcgtcggtgc
     6541 tctccctctg ccggcgctgc gggtgggacg aggtccacag cagccaggta gcccgcctgg
     6601 ccctccagct cttcgacggc acggccgggc tgcaccgtct gggcggggcg gaccgggagc
     6661 tgctcgagca cggcgccctg ctgcacgaca tcggcgagca cgtgtcgccg gaaggtcatc
     6721 acaagcacac cgcctacctc atcgagcacg gccgccttcg cgggttcgag cccgccgagg
     6781 tcgccgtgct cgccacgctc gggcgctatc acctccgggg cgatccgaag gccgtgttcg
     6841 agcccttcgg gcggctcgac accgaccgcc agcgccaggt ccgcgtcctg gtcggactgc
     6901 tccgggtcgc cgacgggctg gaccgcagcc atgccgcgac cgtcggcgac ctcgacgtga
     6961 tggcggggcg gccctcgatc aggatcgtgg tcggggcgca cggcgacccc gagctcgagg
     7021 tgtggggcgc ccgccgcaag ctggacctgc tcgaacgggc cctcgggcgc ccggtgatgg
     7081 tcgacgccgc ccacccgggc actctggctc gccagcgcct ccggggcgcg tgacggggta
     7141 tcgttcgtcc catcgcctca tgatcgacgc gctcccggct ccaggatgtg aaccctctga
     7201 tggttgacag caaactgaag gctcctgtcc ggatcgccgc gccggccccg tcccgggagg
     7261 tgggccgagc cgggcgcgag ccgacgggcg agcccgcctt ctcggcccgg gacgtccggt
     7321 tcttctacgg ctcgcacctg gccctccagg gcgtgaccct ggacatcccc acccggcagg
     7381 tcaccgccct gatcgggccg tcgggctgcg ggaaaagcac cttcctgcgg tgcctcaacc
     7441 ggatgaacga cgagatccgc ggtgcccgga tggaggggtc cgtcacctat cgcggcgtcg
     7501 atctctacgc cgacggcgtc gagcccaccg cagtgcgccg gaccatcggc atggtcttcc
     7561 aacgtcccaa tcccttcccg aagtcgatct acgacaacgt ggcgttcggc gtgcgggctc
     7621 tgggcctgcg caagggcgac ctcgaggacg cggtcgagca ggcgctgcgc cgggtggccc
     7681 tgtgggacga ggtcgccagg aagctcaagg ccagcgccct ggggctgtcc ggcggccagc
     7741 agcaacggct ctgcatcgcc cgggccctcg ccgtggaccc ggacgtgatc ctcatggacg
     7801 agccctgctc cgccctcgat cccatcgcca cgctcaagat cgagcagctc atcggcgagc
     7861 tacgcctcga ttacaccatc gtgatcgtca cccacaacct ccagcaggcc gcccgggtga
     7921 gcgaccgcac cgccttcttc accgttgagg ccaccgggcc gggcgcccgc accgggctgc
     7981 tggtcgaggt cgggcccacc gcccagatct tcggcagctc caaggaccag cgcaccgacg
     8041 actacgtcgc cggccggttc ggctgatctc tcagggatcg tccgggtggg cccagatggt
     8101 ggactcgtcg gcgtcgcccg ggtgcatcac cccggacgcg atgaagcaca cgcgctcgcc
     8161 gatgaccacg gcgtggtcgg cgacccgctc gaagaaccgc ccgaccatgc tgagctccac
     8221 gaccggctcg gctgggacct cgccgaggct gaacagctgc cgtcgcagct ctccgtgggc
     8281 ggcgtccacg gcgtcgtcgt cggccgctag ctgcgacgcc gcctccgcgt cgaacacggc
     8341 gtacgccgcc ccggcccgcc gcaacaggtc gagcgccccg tcgcccaacc ggcggagtgg
     8401 cccctcgagg ccgtggccgg ccaccagcgg gcgcacgacc tcgacccgcc gaccggtgga
     8461 cacgagcagg tcgccgacgc gctcgagggc ctggccgatc cgaaggctgg cggtcaggaa
     8521 gcgcaggtcc ctccccacgg gggactgccg ggcgaccagg tcgaagacct cgccctccag
     8581 ccactcgagc agcgcgtcca cccgggcgtc ggttgccagc acgtcggcca gggcggcgtc
     8641 gggctcggtc tcgccgaccg cggccgccag cacggcgtcg ccggctcccg ccagggtcgt
     8701 gatggtctcg cccgtgcgg
//
LOCUS       DASWGG010000282         1849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2828097, whole genome shotgun sequence.
ACCESSION   DASWGG010000282 DASWGG010000000
VERSION     DASWGG010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2828097"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..520
                     /locus_tag="VF954_05100"
                     /pseudo
     CDS             44..520
                     /locus_tag="VF954_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            670..1461
                     /locus_tag="VF954_05105"
     CDS             670..1461
                     /locus_tag="VF954_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9530493.1"
                     /translation="MGDGEGSDPFTLGGRHALVTGAGQGIGRCIALTFAEHGAGTIVV
                     NDLFQDRADAVAAEIRDAGGRAVAVAADVTDLAAVEAMAEVTRQLDAPVQVLVNNAGL
                     PPGPVQMMPFLETEPAQWEPMLRLNLYGVLQVTRTFAPAMVEAGWGRVITMISDASRV
                     GDPFQAVYAAAKAGAAGFSRSLASEIGKRGVTVNCIALATVLPGYDPAVGLSDDMQRR
                     FKPYAMRRPGRPEDVAPTALLLASEAGQWITGQVYPVNGGYSYAL"
     gene            complement(1485..>1849)
                     /locus_tag="VF954_05110"
     CDS             complement(1485..>1849)
                     /locus_tag="VF954_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530494.1"
                     /translation="TGPKTLALVREFADWWNVHTGAVAKVPELREQVGDARVSIQQVL
                     ALAPRGGDREKVTEVASRRFPYSKPVIGSGPELLDHYGGLAGQGIERVYTWFADFAPP
                     ETVAEFGDEVVARLRGSG"
BASE COUNT          267 a          662 c          662 g          258 t
ORIGIN      
        1 cagcaacgcc tacggcgcca ccgaggcgtc tctgatctcg tggctgccgc caggccggcc
       61 cgggcgacct ggctcggcgg ggatcgtgaa ctccgagacg ttcatcgtga agatcttcga
      121 cgacaacgac cgggaggtcc cggccggccg ggagggcgag atcgtctgcc gcccccggat
      181 gccgcacgtc atgttcgagg gctactggat gcggcccgag gcgaccgtgg ccgcctggcg
      241 gaacctctgg ttccacaccg gcgacatcgg gcggatcgac tccgacggct acctgttctt
      301 cgtcgatcgc aaggccgact acctgcggcg ccggggcgag aacatctcga gctgggaggt
      361 ggagaaggtg ttccactccc accccgacgt gcaggatgta tgcgtgcacg cggtaccgag
      421 tgacgtgggc gaggacgacg tgaaggtgac cgtcgtgctg cgggaggggg aatcgctgac
      481 cgaggagcgg ctgtgccggt gggcgatcga ccagctcccc tacttcgcgg tgccgcgcta
      541 cttcgagttc cgcggcggcc tgccgctcag cgagaccggc cgggcgacca agaacgtcct
      601 gcgggatcag ggtgtcaccc ctgccacctg ggatcgcgag gcggccggcg tgacgatgga
      661 gcgccggtta tgggagacgg cgaggggagc gacccgttca ccctcggcgg ccggcatgcg
      721 ctggtcaccg gcgctggcca gggcatcggg cggtgcatcg ccctcacctt cgccgagcac
      781 ggcgccggca cgatcgtcgt caatgacctg ttccaggacc gggccgacgc cgtcgccgcc
      841 gagatccgcg acgcgggcgg ccgggcggtg gccgtggcgg ccgacgtcac cgacctggcc
      901 gcggtcgagg cgatggccga ggtgacgcgc cagctcgatg cgccggtcca ggtcctggtc
      961 aacaacgccg gcctgccccc cggcccggtc cagatgatgc cgttcctcga gaccgagccg
     1021 gcccagtggg agccgatgct gcgactcaac ctctacggcg tgctgcaggt gacccggacc
     1081 tttgccccgg ccatggtcga ggccggctgg gggagggtca tcaccatgat ctccgacgcc
     1141 tcccgggtcg gcgacccctt ccaggccgtc tacgccgccg ccaaggcggg ggcggccgga
     1201 ttcagccggt cgctggccag cgagatcggc aagcgcggcg tcaccgtcaa ctgcatcgcc
     1261 ctggccacgg tgctccccgg ctacgacccg gccgtcgggc tcagcgacga catgcagcgc
     1321 cggttcaagc cgtacgcgat gcggcgcccg ggccggccgg aggacgtcgc gcccaccgcc
     1381 ctcctgctgg ccagcgaggc cgggcagtgg atcaccggcc aggtgtaccc ggtcaacggc
     1441 ggctacagct acgcactgta gatctgccgc ggggtcgcga gcggtcagcc gctcccgcgg
     1501 aggcgggcca cgacctcgtc gccgaactcc gccacggtct cgggcggggc gaaatcggcg
     1561 aaccacgtgt agacccgctc gatgccctgc ccggccaggc cgccgtagtg gtcgagcagc
     1621 tcgggcccgg agccgatgac gggcttcgag tacggaaagc gcctgctggc gacctccgtg
     1681 accttctcac ggtccccgcc gcgtggcgcc agggcgagta cctgctggat cgacacccgg
     1741 gcgtcgccta cctgctcgcg cagctcggga accttcgcca ccgcgccggt atgcacgttc
     1801 caccagtcgg cgaactcacg caccagcgcc agcgtcttcg gcccggtgc
//
LOCUS       DASWGG010000283         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2828617, whole genome shotgun sequence.
ACCESSION   DASWGG010000283 DASWGG010000000
VERSION     DASWGG010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2828617"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VF954_05115"
     CDS             <1..393
                     /locus_tag="VF954_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530495.1"
                     /translation="LGPGGGRGQPSARAVTVERMRSLSATLGSADGVAAPDAAAGLRR
                     VADSVASGGGRSQAEDLMASVAAWQAEGRLTPTAAGAVEQVLAEVPGAQVPPTTIATT
                     TTIAPAASGHHGHRGDGNGGGAGGGSGD"
     gene            411..1421
                     /locus_tag="VF954_05120"
     CDS             411..1421
                     /locus_tag="VF954_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX9530496.1"
                     /translation="MAALDRIAFILERRREPTYRVRAFRNAASVLRELGPEELTRLSG
                     AGQLRTLPGIGDTTAKVVSEALAGRVPSYLAELQGETEWGPDIGEGGPLLAALRGDCH
                     THSDWSDGGSPIAEMADAARQLGHEYLVLTDHSPRLTVAHGLNPERLRRQLEEVAAVN
                     ERMAPFRVLTGIEVDILEDGSLDQEDDLLARLDVVVASVHSKLRMETRAMTKRMVAAL
                     ANPHTDILGHCTGRLVVGRGRPPSEFDADEVFGAAAAHGKAVEINSRPERLDPPRALL
                     RRALELGCLVSIDTDAHAPGQLTWLPYGCARAAECGVSPDRVVNTMAADDVVAWAAGH
                     DR"
BASE COUNT          201 a          574 c          598 g          178 t
ORIGIN      
        1 ctggggccgg ggggcgggcg ggggcagccg agcgcccggg cggtcacggt ggagcggatg
       61 cggtcgctgt ctgcgacgtt gggcagcgca gacggagtgg ccgccccgga cgccgcggcc
      121 ggcttgcggc gcgtggcgga ctcggtggca tcgggcggag gtaggtccca ggccgaggac
      181 ctcatggcgt ccgtcgcggc ctggcaggcg gagggccggc tgaccccgac ggccgcaggc
      241 gccgtcgagc aggtgctggc ggaggtccca ggcgcgcagg tcccaccgac gaccatcgcc
      301 acgacgacga ccatcgcacc ggcggcgtcc ggccaccacg gccaccgggg tgacggcaac
      361 ggtggcggcg ccggtggcgg cagcggggac tgaggctcgg cttggacccc gtggcggcgc
      421 tggaccgcat cgccttcatc ctcgagcgcc gccgcgagcc gacctaccgg gtccgggcct
      481 tccgtaacgc cgcctcggtg ctgcgcgagc tgggcccgga ggagctgacc cggctgtccg
      541 gggccgggca gctgcgcacg ctgcccggca tcggcgacac caccgccaag gtcgtgtcag
      601 aagcgctcgc cggtcgcgtg ccgtcctacc tggccgagct gcagggcgag acggagtggg
      661 gccccgacat cggcgagggc gggcccctgc tggccgcgct gcggggcgac tgccacaccc
      721 actccgactg gtccgacggc ggcagcccga tcgccgagat ggccgacgcg gcacgccagc
      781 tgggccacga gtacctggtc ctcaccgacc actcgccccg cctgacggtc gcccacgggc
      841 tcaaccccga gcgtctccgc cgacagctcg aggaggtcgc ggcggtgaac gagcggatgg
      901 cgccgttccg ggtcctgacg gggatcgagg tcgacatcct ggaggacggt tccctggacc
      961 aggaggacga cctgctcgcc cgcctcgacg tggtggtcgc cagcgtgcac tcgaagctgc
     1021 ggatggagac gcgggccatg acgaagcgca tggtcgccgc cctggccaac ccccacaccg
     1081 acatcctggg ccactgcacg ggaaggctcg tcgtgggccg gggccggccc ccgtcggagt
     1141 tcgacgccga cgaggtcttc ggcgccgccg ccgcccacgg caaggcggtg gagatcaaca
     1201 gccggccgga gcggctcgac ccgccccggg cgttgctgcg ccgcgccctc gagctcggct
     1261 gcctcgtctc gatcgacacc gacgcccacg ccccagggca gctcacgtgg ctcccgtacg
     1321 gctgcgcccg ggcggccgaa tgcggggtct cgcccgatcg ggtcgtcaac accatggcag
     1381 ccgacgacgt ggtggcctgg gcagccggcc acgaccggta gctgcgccac ggggccaggg
     1441 cacggggggc gcggtcggcg ggcgtaggat cgccgtctgc ccatgggccc cgccgaacgc
     1501 ttcaccgccc tcgtccgccg gccggaggag ctggtacccc tcgacgaggc g
//
LOCUS       DASWGG010000284         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2832269, whole genome shotgun sequence.
ACCESSION   DASWGG010000284 DASWGG010000000
VERSION     DASWGG010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2832269"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VF954_05125"
     CDS             complement(<1..207)
                     /locus_tag="VF954_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX9530497.1"
                     /translation="MSLPLPSTLSPSKMAAFKECALAFRLGAIDKLPEPPSPAATRGS
                     LVHRALQELFWSHPAGARTVAASEA"
     gene            complement(256..1125)
                     /locus_tag="VF954_05130"
     CDS             complement(256..1125)
                     /locus_tag="VF954_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9530498.1"
                     /translation="MAPAPARITTSDGVDLAVHDLGGDGPPLLMAHATGFHGQVFAPL
                     ARLLAPFFHCWSFDFRGHGDSAGPPGGDFAWAGFGRDVIGVVDGVGLVRPFSVGHSAG
                     GAGLLIAELDRPGTFRAMYAYEPIVFPPGPGPSAQASGAQPNRGHLLAASARRRREVF
                     ASRAEALANFAAKPPLADLDPLALEAYVAHGFEDLPDGSVRLKCRREDEARVYEMAGQ
                     HGTYDRLGQIRCPVTLACGARTESLGEDAMRAAAQRMPAARVDVLEGLGHFGPMQDPA
                     AVAAGVHHAFGGS"
     gene            1306..1689
                     /locus_tag="VF954_05135"
     CDS             1306..1689
                     /locus_tag="VF954_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9530499.1"
                     /translation="MAANRRFYEAFEHGDIDAMSDIWEHSDRALCTHPGWPTLRGWAA
                     ISASFYSLFRGGSAMQFVLTNEQAEVVGEAAWVSVDENLLGDQGGATLAAINVFVQSE
                     GGTGWRMVCHAGSVVSFPIGEGPRR"
     gene            complement(1655..2602)
                     /locus_tag="VF954_05140"
     CDS             complement(1655..2602)
                     /locus_tag="VF954_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX9530500.1"
                     /translation="MVDRAFPPGRVLTGAASWSDRSLVRDGGWYPRRTMTATERMAFY
                     AARLPVVEIDSTFRFPPTPDVCRQWADRTPDGFTINVVAWSLLTANSALPDSLWPDLQ
                     TEVRPELQDRRRLYAHHLNDDAWAECWTRFRHAIEPLVRAGRLGGVMLRYPHWLRPGD
                     TGRALLRRARQGLPGVPLAAHFANPHWLEGDQCEATLGFLEDHSLGLVCTDDGGALER
                     QPVVAATAELAVVRFEGCSPGRWDDPEMPWPERFAYRYCDDELSPWVPRLQALASSAS
                     AVHAVFANTWRDDAVAGALRLAALLDDPVTVGGLHRSES"
BASE COUNT          400 a          924 c         1008 g          328 t
ORIGIN      
        1 ggcctcggag gcggcgacgg tgcgagcccc ggccgggtgg gaccagaaca gctcctggag
       61 ggcgcggtgg accagggacc ctctggtcgc ggccggtgac gggggctcag ggagcttgtc
      121 gatggcgccc agccggaagg ccagggcgca ctccttgaac gcggccatct tcgagggtga
      181 cagcgtggac gggaggggca agctcatcgc agccaagggt aagccgggcg ggtgacgtcg
      241 cggtggtacc cccgctcacg acccgccgaa ggcgtggtgc accccggcgg ccaccgccgc
      301 cgggtcctgc atgggcccga agtggccgag cccctcgagc acgtcgaccc tcgccgccgg
      361 catgcgctgg gcggccgccc gcatagcgtc ctcacccaag gactccgtgc gcgccccgca
      421 ggccagggtg accgggcagc ggatctggcc cagccggtcg taggtcccgt gctggccggc
      481 catctcgtag acccgggcct cgtcctctct ccggcacttc agccgcacgg agccgtctgg
      541 caggtcctcg aagccgtggg ccacgtaggc ctcgagggcg agcgggtcga ggtcggccag
      601 tggcggcttg gccgcgaagt tggcgagcgc ctcggcccgg gacgcgaaga cctcccgccg
      661 gcgccgggcc gaggcggcca ggaggtggcc ccggttgggc tgcgccccgg atgcctgcgc
      721 ggacggcccg ggccccggcg ggaacacgat cggctcgtag gcgtacatgg cccggaaggt
      781 gccggggcgg tccagctcgg cgattagcag accggcccca ccggcggagt gcccgaccga
      841 gaagggccgg acgaggccca cgccgtcgac cacgccgatc acgtcccggc cgaagccggc
      901 ccaggcgaag tcgccccccg gcgggccggc agagtcgcca tggccccgga agtcgaacga
      961 ccagcagtgg aagaacggcg ccaggagccg ggcgagcggg gcgaacacct ggccgtggaa
     1021 gccggtggcg tgggccatga gcagcggcgg cccgtcaccg cccagatcgt gcacggcgag
     1081 gtcgacgccg tcggacgtgg tgatgcgggc gggggctggc gccacaccgt gcgaccttag
     1141 tgccaagcgc aaaactactt cgggcggctt ctttggactc aggtgtcgcg agaccgggaa
     1201 gatggcctac ggccgcccaa gtagctttgc gttcggcact aaccggcgaa aggtagcgtc
     1261 gggcccatcg acaccccgga ccccgcccca gacgtcgcct cggtcctggc ggccaaccgc
     1321 aggttctacg aggccttcga gcacggtgac atcgacgcca tgtccgacat ctgggagcac
     1381 tccgaccgcg ccctgtgcac ccaccccggt tggcccaccc tgagggggtg ggcggccatc
     1441 tcggcgtcgt tctacagcct gttccgcggg ggttcggcga tgcagttcgt gctgaccaac
     1501 gagcaggcgg aggtggtggg cgaagccgcc tgggtgtcgg tggacgagaa cctgctcggc
     1561 gaccagggag gcgccaccct ggccgccatc aacgtgttcg tccagagcga agggggaaca
     1621 ggatggagga tggtctgcca tgcagggtcg gtcgtcagct ttccgatcgg tgaaggcccc
     1681 cgacggtgac cgggtcgtcg agcagcgccg ccagccggag ggcaccggcc acggcgtcgt
     1741 cacgccacgt gttggcgaac acggcgtgca ccgcgctcgc ggacgaggcc agggcctgca
     1801 acctgggcac ccacgggctc agctcgtcgt cgcagtagcg gtatgcgaac cgttccggcc
     1861 acggcatctc ggggtcgtcc cagcggcccg ggctgcagcc ctcgaagcgc acgaccgcca
     1921 gctcggcggt ggcggccacg acgggctggc gttcgagggc gccgccgtcg tcggtgcaga
     1981 ccagaccgag ggagtggtcc tccaggaacc cgagggtggc ctcgcactgg tcgccctcca
     2041 gccagtgggg gttggcgaag tgcgcggcca gcggaactcc cggaagcccc tggcgggctc
     2101 ggcgcagcag ggcgcggccg gtgtcaccgg gacgcagcca gtgggggtac cgcagcatga
     2161 cgccgcccag ccgcccggcc cgcacgaggg gctcgatggc gtggcggaac ctcgtccagc
     2221 actccgccca ggcgtcgtcg ttgagatggt gggcgtacag ccggcgccgg tcctgcagct
     2281 cggggcgcac ctcggtctgg agatccggcc agagcgagtc gggcagggcg gagttggcgg
     2341 tgaggaggga ccaggccacc acgttgatcg tgaacccgtc cggggtccgg tccgcccact
     2401 ggcggcagac gtcgggagtc ggcgggaagc ggaaggtgct gtcgatctcg accaccggca
     2461 gccgggccgc gtagaacgcc atccgctccg tcgccgtcat ggtgcgccgc gggtaccagc
     2521 ccccgtcgcg gaccagcgac cggtccgacc acgacgccgc gccggtcagg actcggccgg
     2581 gcgggaaagc acgatccacc attccacctt gccacgcgcg ccagggaagg cgtggtgacg
     2641 ctcgatccac agatagaccc
//
LOCUS       DASWGG010000285         4439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1236417, whole genome shotgun sequence.
ACCESSION   DASWGG010000285 DASWGG010000000
VERSION     DASWGG010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236417"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..573
                     /locus_tag="VF954_05145"
     CDS             142..573
                     /locus_tag="VF954_05145"
                     /inference="COORDINATES: protein motif:HMM:NF025382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4232 domain-containing protein"
                     /protein_id="HEX9530501.1"
                     /translation="MDELNVTSPGTQGAAGHQAAVIVFENRGSRTCVLTGYPGVAALD
                     QSGHQVAQAERTPTGSIGGLSSAGEALPVVTVAPAGKASATVEGTSVPVGGASGCPTY
                     SALEVTAPGETRSARLDVGLPGCSPLQVHPVVAGTTGSERH"
     gene            923..2494
                     /locus_tag="VF954_05150"
     CDS             923..2494
                     /locus_tag="VF954_05150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX9530502.1"
                     /translation="MALEEVAPQAPTAIDEYRMRTVRVAVAATYVAVALLLCIPILPG
                     RADINTVPYGFLVGLTAVGGTAAALLPWRRLFAAGWGQRLLYGWSVLDIVLVTSGMAV
                     SGGPQSDVIFLYVLTTVFFAISYTRGPQAWLFAMTCAAYLLLVLGWHAGTPVAAVVVR
                     LGTLATVWFMVAYLSQERNAQLSAYLSSQELAEHRAELLRAVARTAASITILDADRVM
                     DEVTTSLIDLGFDVCNFDVLEEGARTYRVSHARGLPEAFTEAVHPAAMGIPGLVMARR
                     GTVVLEDYNSYALAVPALIHLGVQVVVGVPVFVDGQPEAVLVAARLHTRHLPAPDIEV
                     VEILATQVGRALENAKRFRAEHDVAEAASLASLRDELTGVGNRRQANLMLERLRPGDA
                     LVLIDLDHFKEVNDRDGHAAGDKVLRVLGQHLRDAVRDGDDVARYGGEEFLLLLRRAG
                     AEAFPAVERILESWRARHMATSFSAGVAFHDGNRPATITIGQADAALYAAKRTGRNRV
                     CMYGSGLEDDEVERV"
     gene            2560..3096
                     /locus_tag="VF954_05155"
     CDS             2560..3096
                     /locus_tag="VF954_05155"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530503.1"
                     /translation="MMRKAVTAGCSVVAVLLAGCGSSAKTSTTSATTSATTPATTPPV
                     SSPTTAGAPSANATVKLSQSSLGSIVTDSAGRTLYLLDKDTPTMATCTGACASTWPPL
                     AAAGAPTAGANLDAAKLSTLTRADGTVQVEYAGHPLYHYSGDASPGDTNGEGFGGVWF
                     AVTAGGTAAQKPSTGGGY"
     gene            3307..>4439
                     /gene="egtB"
                     /locus_tag="VF954_05160"
     CDS             3307..>4439
                     /gene="egtB"
                     /locus_tag="VF954_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX9530504.1"
                     /translation="MAVATSAPAPALDQGQRFASVRRLTETLASPLSPEDQTVQTMAD
                     VSPTKWHRAHTSWFFETFLLEPSFPGYRAFDPEYGYLFNSYYEAIGSRHPRAERGLLS
                     RPGIAEIAAYRRHVDEAMAALIESLPLDPVPPAAGLVELGLHHEQQHQELLLMDIKHV
                     LSRNPLQPGYQCPADGPRRDAQPSARPSRSNPSLGWLTHAGGLADVGAGAEGFAFDNE
                     RPRHVAHLEPFAIADRPVTCGDWLEFLADGGYERPECWLSDGWAAVRSGLIQAPLYWS
                     DGHRGWELFTLRGPQPVDPAEPVCHVSYYEADAYAHWAGARLPTEVEWEAVLGPFVGG
                     RDEIAYHPGPLTGDPPFVGAVWEWTASAYLPYPRFAPAPGAVG"
BASE COUNT          587 a         1718 c         1506 g          628 t
ORIGIN      
        1 tgcacgtcga cccaccatgg cggcgcaccc gccaccacct ccacggcgcc gaccgcctct
       61 gcgacgacga gcacgagcgc ctcgacgacc acctcgggac cagctcggac gacgaccacc
      121 gcaagaggcg ccggttgcac gctggacgag ctgaacgtga catcgcccgg cacgcagggc
      181 gccgcgggtc atcaggccgc ggtcatcgtc ttcgagaacc ggggcagccg gacgtgcgtc
      241 ttgaccggct acccgggcgt ggcggctctt gaccagtcgg gccaccaggt agcccaggcc
      301 gagcggactc cgaccggctc catcggcggt ctctcgtcgg ccggtgaagc cctcccggtg
      361 gtcaccgtgg cgcccgccgg caaggcctcg gcgacagtgg agggcacgtc ggtcccggtc
      421 ggcggggcgt cgggttgccc gacctacagc gccctggaag tcacggcgcc gggggagacc
      481 cgctccgccc gcctggacgt cggcctcccg gggtgcagcc cgctccaggt ccaccccgtc
      541 gtggccggga cgacgggcag cgagcgccac tgaaggtcca aggcggtacc ctcacgccgg
      601 tgtccagccc cgacgcccgg gaggagcctg cgatccagcc gcccgggcat cgtggtgtcc
      661 atgcgcgacg tctccgatcg caacgccgcg gcccgagtcg agcgggccgc tcacgaggcc
      721 aggcgcggct tcgtcgccct ggcctcgggc tggcggtggc gccagccctg gcccgggcca
      781 acggtggcga cacctggtac gagcccaagg cgccggccgg gtcgaggttc ggggtccggc
      841 tgacgactcc cggggattga cgccggcggc gggaccgggg cctccgcggg ctccgcgggg
      901 gtgaagcctc tacccttgcg agatggctct ggaggaggtc gcgccccagg cgccgaccgc
      961 catcgacgag taccggatgc gcaccgtccg ggtggcggtg gcggccacct acgtcgccgt
     1021 cgccctgctg ctctgcattc ccatccttcc tggtcgggcc gacatcaaca ccgtgccgta
     1081 cgggttcctg gtcgggctca cggccgtcgg cggcacggcc gccgccctcc ttccctggcg
     1141 gcgcctcttc gcagccggat ggggccagcg gctcctctac ggctggtcgg tgctcgacat
     1201 cgtcctggtc acgtcgggga tggccgtctc cggtggtccg cagtccgacg tcatcttcct
     1261 ctacgtcctg accacggtgt tcttcgccat ctcctacacg cgtggccccc aggcgtggct
     1321 ctttgccatg acctgcgccg cgtacctgct cctcgtgttg ggatggcacg ccggcacccc
     1381 ggtggccgcg gtggtcgtgc gcctcggcac cctcgccacc gtgtggttca tggtcgccta
     1441 cctctcccag gagcgcaacg cccagctctc ggcctacctg tcgtcccagg agctcgccga
     1501 gcaccgcgcc gagctgctcc gcgccgtggc ccgcaccgcg gcgtcgatca cgatcctcga
     1561 cgccgatcgg gtcatggacg aggtgaccac ctcgctcatc gacctgggct tcgacgtctg
     1621 caacttcgac gtgctcgagg agggcgcccg cacctaccgg gtcagccacg cacggggcct
     1681 gccggaggcg ttcaccgagg ccgtgcatcc cgcggccatg ggcatacccg gcctggtcat
     1741 ggcccggcgg gggaccgtcg tgctcgagga ctacaactcc tacgccctgg ccgtgccggc
     1801 gctgatccac ctcggcgtcc aggtggtcgt cggcgtcccc gtcttcgtgg acggccagcc
     1861 cgaggccgtg ctcgtcgccg cccgcctcca caccaggcac ctcccggcgc cggacatcga
     1921 ggtggtcgag atcctcgcaa cgcaggtggg ccgggccttg gagaacgcca agcgtttccg
     1981 ggccgagcac gacgtggccg aagccgccag cttggcgtcc ctgcgcgacg agctcaccgg
     2041 tgtgggcaac cggcgccagg ccaacctcat gctcgagcgg ctgcggccgg gcgacgcgct
     2101 ggtgctgatc gatctcgacc acttcaagga ggtcaacgac cgagatggcc acgccgcagg
     2161 ggacaaggtg ctgagggtcc tcggtcaaca cctgcgcgac gcggttcgtg acggcgacga
     2221 cgtggcccgt tacggcggcg aggagttcct gttgctcctg cgccgggccg gcgccgaagc
     2281 cttccccgcg gtcgagcgga tcctcgagtc ctggcgcgcc aggcacatgg ccaccagctt
     2341 cagcgccggc gtcgccttcc acgacggcaa ccgcccggcg accatcacca tcggccaggc
     2401 ggacgccgcc ctctacgcgg ccaagcgcac ggggcgcaat agggtctgca tgtacggcag
     2461 cggcctggag gacgacgagg tcgagcgggt atagccgcca ccacggcgat ctgttcgggc
     2521 ccggcacccg tacctacggg catgcgaaag gatggttcca tgatgcgcaa ggctgtgact
     2581 gccggctgtt ctgtagtcgc cgtgctgctc gccggctgcg gcagcagcgc caagacctct
     2641 acgacatcgg cgacgacatc ggcgacgaca ccggccacga ccccgccggt gagctcgccc
     2701 acgaccgcgg gcgctccgtc ggcgaacgcg acggtcaagc tgagccagag ctcgctcggc
     2761 tccatcgtca ccgactcggc cggacgcacc ctctacctgc tcgacaagga cacccccacc
     2821 atggccacct gcacaggggc gtgcgcctcc acgtggccgc cgctcgcggc cgcgggagcg
     2881 cccaccgccg gcgccaacct ggacgccgcc aagctctcga ccctcacgcg cgccgacggc
     2941 actgtgcagg tcgagtacgc cggccacccc ttgtaccact actccggcga cgcctcgccg
     3001 ggcgacacca acggtgaggg cttcggcggc gtgtggttcg ccgtgacggc cgggggcacc
     3061 gccgcccaga agccgtcgac cggcgggggg tactagctgg ccgcccgggg aggcggcgtc
     3121 gccgaactag cccggcttgg ggcgtagggt cttgtggacc gtccctctct ccccccggag
     3181 gccccccatg ccacgccacg cacccgcccg gccctgctgg aggccccgcc cgccgagcag
     3241 cgtcacgcct tggctcgccc ccgtccgcaa ccaggactgg gtcgccgccc agatcggcgt
     3301 cgggtaatgg ccgtggccac ctccgcgccc gcaccggccc tcgaccaggg gcagcgcttc
     3361 gcctccgtcc ggcgcctgac cgagacgctg gcatcgccgc tctcgcccga ggaccagacc
     3421 gtccagacca tggcggacgt gagcccgacc aaatggcacc gggcccacac ctcgtggttc
     3481 ttcgagacct tcttgctcga accgtccttc cctggctacc gtgcgttcga cccggagtac
     3541 ggctacctgt tcaactccta ctacgaggcg atcgggtccc ggcacccccg agccgagcgg
     3601 ggcctgctgt cccgcccggg gatcgccgag atcgccgcct accgccgcca cgtcgacgag
     3661 gccatggccg cgctgatcga gtcgctgcca ctcgaccctg tcccgcccgc cgccgggctg
     3721 gtcgagctgg gactccacca cgagcagcag caccaggagc tgctcctcat ggacatcaag
     3781 cacgttctgt cgcgcaaccc gctccagcct gggtaccaat gcccggccga cggcccccgg
     3841 cgcgacgccc agccctccgc tcggccgagc cggtccaacc cgtcgttggg atggctgacc
     3901 catgccggtg ggctggccga cgtcggcgcc ggagcggagg gcttcgcctt cgacaacgag
     3961 cggccccgcc acgtggccca cctcgagccg ttcgccatcg ccgaccgccc ggtcacctgt
     4021 ggcgactggc tcgagttcct cgccgacggc gggtacgagc gacctgagtg ctggctttcc
     4081 gacggctggg ccgcggtgcg gtccgggctc atccaggccc ccctctactg gtccgacggg
     4141 caccgggggt gggagctctt cacgctccgc gggccccagc ccgtcgaccc ggcggagccg
     4201 gtgtgccacg tgtcctacta cgaggcggac gcctacgctc actgggccgg ggcccgcctg
     4261 cccaccgagg tcgagtggga ggccgtgctg ggccccttcg tcggcgggcg cgacgagatc
     4321 gcctaccatc ccgggccgct caccggggac ccgcccttcg taggggcggt gtgggagtgg
     4381 acggcttcgg cctacctgcc gtacccgcgg ttcgcccccg ctcccggcgc ggtcggcga
//
LOCUS       DASWGG010000286         1937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2834485, whole genome shotgun sequence.
ACCESSION   DASWGG010000286 DASWGG010000000
VERSION     DASWGG010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2834485"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..908
                     /locus_tag="VF954_05165"
     CDS             129..908
                     /locus_tag="VF954_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530505.1"
                     /translation="MPKTPLDRPHEWISFADPGEDRTWVFDVTFLLSPWTCIFGRGCQ
                     GVLTGPAPELSQGCCSYGAHLTDEADVARVTAAAATLTASQWQFRARGRKAGISRTNA
                     DGDVVTREVEGACVFLNRPGFAAGPGCALHRAALDRGVPPLSLKPNVCWQLPLRREDE
                     VAGTGHVTSTVRQWDRRDWGDGGFEFHWWCTEAPEAFAGHRPVYQELRDELVELVGPE
                     VYDLVAGYLSGRSAGPASPLVHPAVAVAAPKRRKREKAAKA"
     gene            complement(949..1122)
                     /locus_tag="VF954_05170"
     CDS             complement(949..1122)
                     /locus_tag="VF954_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530506.1"
                     /translation="MKKGRHRRFEEARDLKRAPKEIVEPCPECGAMPGADHATWCLAE
                     QDQDVEEEQGGAD"
     gene            1411..1914
                     /locus_tag="VF954_05175"
     CDS             1411..1914
                     /locus_tag="VF954_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9530507.1"
                     /translation="MADERDYLATYAEAAPDRLAVVEDDVVLDYATFNRRVNRMANVL
                     AGAGVRTADKVLWVGQNSADVVVVVNALRKVKAVGVPMNYRLTAEEAQYVIDNSDSVA
                     VLFDPEQTAQLDGLQDQCPKVKHWLAFRCPPGDQPGWATNLDDRMAVADEGEPVSHGE
                     DGTAGRR"
BASE COUNT          257 a          710 c          689 g          281 t
ORIGIN      
        1 cggtgaccag ctgcgctgcg ggtcgcccgt gcacgtctcc agactgtcac gtgccccgtg
       61 cgagggccat gacctcggcc cgcggtcatc gcggccggcg ccgggcgtca cgtactgtgg
      121 gcccgacgat gccgaagacc cccctcgatc ggccccatga gtggatcagc ttcgccgacc
      181 cgggcgagga ccggacctgg gtcttcgacg tcaccttcct gctgtccccg tggacgtgca
      241 tcttcggccg gggctgccag ggcgtgctga ccggacccgc gccggagctg tcgcagggct
      301 gttgctccta cggggcgcat ctcaccgacg aggccgacgt ggcccgggtg accgcggccg
      361 cggccaccct gacggcgagc cagtggcagt tccgcgccag gggccgaaag gcgggcatct
      421 cccggaccaa cgccgacggc gacgtggtca cgcgtgaggt cgagggcgcg tgcgtgttcc
      481 tcaaccgtcc cggcttcgcc gccgggcccg gctgcgccct gcaccgggcc gctctggacc
      541 ggggggtgcc accgctgtcg ctgaagccca acgtgtgctg gcagctgccc ctccgccggg
      601 aggacgaggt ggcgggcacg ggacacgtga cctcgacggt gcgacagtgg gaccggcggg
      661 actggggcga cgggggcttc gagttccact ggtggtgcac cgaggccccc gaggccttcg
      721 ccggccaccg gcccgtgtac caggagctac gcgacgagct ggtcgagctg gtgggccccg
      781 aggtgtacga cctggtcgcc ggctacctct ccggccgctc ggccggcccg gcctccccgc
      841 tcgtgcaccc cgcggtggcg gtggccgcgc cgaagcggcg caagcgcgag aaggctgcaa
      901 aggcctgagc ccgaacgccc cgtcaggccg gcccgtcagg ccgccccgtc agtcggcgcc
      961 cccctgctcc tcctcgacgt cctggtcctg ctcggccagg caccacgtgg cgtggtcggc
     1021 gccgggcatg gcaccgcact ccgggcaggg ttcgacgatc tccttgggtg cacgcttgag
     1081 gtcgcgcgcc tcttcgaagc ggcggtgccg tcccttcttc acgtcgtgct ccccggtgtc
     1141 gagaacggtc aggactccgg cgcattctag ccgcggtcag gggcggccca ggacccggtc
     1201 cacccggcta cccggcgggc cgtccccctt gctcccgggg tttgcccggt gggggttcgg
     1261 gaaggcttgc ggtatggaac tgttcctcgc ggtcgtggtg gtcgccatga tgctcttcgg
     1321 cggccaccgc tactggtacg accgctcccg aggctgagag gcccacgccc ttcgcgtgta
     1381 ggttgaccca cgcacgcgag ggggacacct gtggccgacg aacgcgacta tctggctacc
     1441 tacgctgaag ccgcgcccga tcgcctggcc gtggtcgagg acgacgtcgt actcgactac
     1501 gcgacgttca accgccgggt caaccggatg gccaacgtcc tcgccggggc cggcgtccgc
     1561 accgccgaca aggtgctgtg ggtgggccag aacagcgccg acgtcgtcgt ggtggtgaac
     1621 gccctgcgca aggtcaaggc cgtcggcgtc ccgatgaact accgcctcac cgcagaggag
     1681 gcgcagtacg tcatcgacaa ctccgactcg gtcgccgtgc tcttcgaccc ggagcagacc
     1741 gcccagctcg acggcctcca ggaccagtgc ccgaaggtca agcactggtt ggccttccgc
     1801 tgcccgccgg gcgaccagcc cgggtgggcc accaacctcg acgaccggat ggcggtcgcc
     1861 gacgagggcg agcccgtgtc gcacggcgag gacggaaccg ccgggcgacg atgatctaca
     1921 cgtccggcac caccggc
//
LOCUS       DASWGG010000287         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2834749, whole genome shotgun sequence.
ACCESSION   DASWGG010000287 DASWGG010000000
VERSION     DASWGG010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2834749"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1685
                     /locus_tag="VF954_05180"
     CDS             <1..1685
                     /locus_tag="VF954_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX9530508.1"
                     /translation="YAELYGPTVGDRVRLADTDLWISPTEDRCTPAGPGDETVFGGGK
                     VMRESMGQALTSTVDLVITNAVVLDHWGVVKADVGVRNGRITAVGKAGNPDIMDGVHP
                     DLVVGPDTEVLAGEGRILTAGIVDCHVHLICPQVIEVALAAGTTTLVGGGTGPPEGTR
                     ATTVTPGAWNLALMLKALDRWPVNVALLGKGNTVSQEALEAQARAGASGYKLHEDWGT
                     TPAAIDACLTACDRLGVQVAIHTDTLNEAGFVADTLAAIAGRSIHAYHTEGAGGGHAP
                     DIITVASAPNVLPSSTNPTRPHTTNTIDEHLDMLMVCHHLNPKVPEDLAFAESRIRPS
                     TIAAEDWLHDLGAISMIGSDSQAMGRIGEVATRTWQTAHVMKQRAGALPGEPVAGADN
                     LRAQRYVAKITICPAVAHGLDGEIGSVEAGKLADLVLWDPGWFGIRPDVVVKGGLVAR
                     AQMGDANASIPTPQPIMSRPMFGDTAAGALSVAWVAPIAIDDGLAERLGLSRRLLPVA
                     NTRSLTKSDLPQNTATPLIEVDPSTFAVRIEGELVKPDPVAVLPLAQRYCLF"
BASE COUNT          271 a          711 c          619 g          244 t
ORIGIN      
        1 gctacgcgga gctgtacggc cccacggtcg gcgaccgcgt ccgtctggcc gacaccgacc
       61 tgtggatcag cccgaccgag gaccgctgca ctcccgccgg gcccggtgac gagaccgtct
      121 tcgggggcgg caaagtcatg cgcgagtcga tgggccaagc gctgacgtcc accgtcgacc
      181 tggtcatcac caacgccgtc gtcctcgacc actggggcgt cgtgaaggcg gatgtgggcg
      241 tgcgcaacgg gcgcatcacc gccgtcggca aggccgggaa ccccgacatc atggacggcg
      301 tccaccccga cctcgtcgtc ggccccgaca ccgaggtgct ggcgggcgag gggcgcatcc
      361 tcacggcggg catcgtcgac tgccacgtcc acctgatctg cccgcaggtg atcgaggtgg
      421 cgctcgcagc cgggacgacg acgctcgtgg ggggcggcac cgggccgccg gaggggaccc
      481 gggcgacgac cgtcacgccg ggggcctgga acctcgccct catgctgaag gccttggacc
      541 gctggccggt caacgtcgcc ctccttggga agggcaacac cgtgtcgcag gaggccctcg
      601 aagcccaggc ccgggcaggg gcgtcgggct acaaactcca cgaggactgg gggacgacgc
      661 ccgccgccat cgacgcctgc ctcaccgcgt gcgaccgcct cggcgtccag gtcgccatcc
      721 acaccgacac gctcaacgag gcgggcttcg tcgccgacac cctcgccgcc atcgccggga
      781 ggtcgatcca cgcctaccac accgagggcg ccgggggcgg gcacgccccc gacatcatca
      841 ccgtagcgtc cgcccccaac gtcctgccgt cctcgacgaa cccgacccgg ccgcacacga
      901 ccaacaccat cgacgagcac ctcgacatgc tgatggtctg ccaccacctg aacccgaagg
      961 tccccgagga cctcgccttt gccgagagcc gcatccgtcc ctcgaccatc gccgccgagg
     1021 actggctgca cgacctcggc gccatctcga tgatcggttc ggactcgcag gcgatgggcc
     1081 gaatcggcga ggtcgccacc cgcacctggc agaccgccca tgtgatgaag caacgggccg
     1141 gcgccctgcc cggcgagccc gtggccgggg ccgacaacct gcgggcgcag cgctacgtcg
     1201 ccaagatcac catctgcccg gccgtcgccc acggcctcga cggcgagatc ggctcggtcg
     1261 aggcgggcaa gctcgccgac ctcgtcctgt gggacccggg ctggttcggc atccgccccg
     1321 acgtcgtggt gaaggggggc ctcgtcgccc gggcacagat gggcgacgcc aacgcctcca
     1381 tcccgacccc ccagcccatc atgtcccgcc cgatgttcgg cgacaccgct gcgggcgccc
     1441 tgtccgtcgc gtgggtggcc ccgatcgcca tcgacgacgg tctggcggag cgtctcggcc
     1501 tctcccggag gctgctgccg gtcgccaaca cgcgcagcct cacgaagtcg gacctcccgc
     1561 agaacaccgc gacgccgttg atcgaggtgg atccgtcgac cttcgcggtg cggatcgagg
     1621 gggagctggt caagcccgac ccggtggccg tcctgccgct cgcccagcgc tactgcctct
     1681 tctagcggca tgacctcgac cgcgctgttg ctgctcgccg atggacggtt cccgggtggg
     1741 ggccacgccc actcgggcgg cgtcgagccg gcggtcgccg acggctcggt gttcgacctg
     1801 gcgtcgctgg agagcttcct cgccggccgg ctcctgacca ccggc
//
LOCUS       DASWGG010000288         6680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_447572, whole genome shotgun sequence.
ACCESSION   DASWGG010000288 DASWGG010000000
VERSION     DASWGG010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447572"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /gene="hisG"
                     /locus_tag="VF954_05185"
     CDS             complement(<1..829)
                     /gene="hisG"
                     /locus_tag="VF954_05185"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX9530509.1"
                     /translation="MLRLVLPKGSLEKATLELFEAADLAVTRSSEVEYRASIDDPRVA
                     YVRILRPQEIPRYVAEGLFDLGITGRDWIEETGSEVVSLGQLHYSKATARPANIVLAV
                     AADSPWATVKDLPAEGLRVSTEYPELTRRFLEKHGISADIRMSYGATEAKIPDIADVV
                     VDLTETGRALRAAGLRVIDTILSSYSELIANPVAAGDAEKRHAMAQLHTLLSGALEAR
                     AKVLVKLNVAEADLDGVIGVLPSMKSPTVSKLFGTDGYAVEAVVPKAQINILIPALKD
                     "
     gene            complement(831..1334)
                     /gene="ribH"
                     /locus_tag="VF954_05190"
     CDS             complement(831..1334)
                     /gene="ribH"
                     /locus_tag="VF954_05190"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX9530510.1"
                     /translation="MTSSERRYEGQMRGDGLRVALACSRFNDFITERLLAGARDGLLR
                     HGVDPASITEAWAPGAFELPLVAQRLAASGEYDAVICLGAVIRGATGHYELVAGQCAA
                     GVAAAGLSTGVPVVFGVLTTETVEQAIERAGTKAGNKGYEAAETAIEMADLLRQLPKP
                     QVGKEGA"
     gene            complement(1321..2544)
                     /locus_tag="VF954_05195"
     CDS             complement(1321..2544)
                     /locus_tag="VF954_05195"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX9530511.1"
                     /translation="MPFAKIEAAIDAVARGEVVVVVDDEDRENEGDLIMAAEFATPEK
                     IAFFLAHTSGLICVPLTGERLDDLDLPLMVSTNTESQRTAFTVTVDYRHGTTTGISAA
                     DRASTIRSLIDAATRPNDLSRPGHILPLRYRPGGVLKRAGHTEAAVDLARAAGLYPAG
                     VLCEVVTHDKTGMARLEELELFAEEHGLLLISIADLIRHRRQKDKLVRRIAEARLPVG
                     GYGDFTCYAYESVINRDQHLALVKGDVHGEDNVLVRVHSECLTGDVFGSLRCDCGPQL
                     DAALRLIATEGRGAVVYLRGHEGRGIGIAHKLRAYTLQEQGRDTVDANLELGLPVDSR
                     EYGIGAQILVDLGITTMRYMTNNPAKYGGLEGFGLEMVERVPLETVPNPENIAYLRTK
                     RERMGHLLEGLDDVL"
     gene            complement(2546..3115)
                     /locus_tag="VF954_05200"
     CDS             complement(2546..3115)
                     /locus_tag="VF954_05200"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX9530512.1"
                     /translation="MFTGIVEELGCLEGRDGARFRFAGPLVAGDAAPGDSIAVNGCCL
                     TVVAVGQGWWEADAVDETLARTNLGDLRAGDGVNLERPVRPTDRLGGHIVQGHVDGVG
                     EVVKPAPDLRVRAPEGLRRYLVEKGSVTVDGCSLTVASVLDDGFAVAVIPHTAAVTTL
                     GRRCPGDRVNLEVDILAKYLERLVLERER"
     gene            complement(3115..4095)
                     /gene="ribD"
                     /locus_tag="VF954_05205"
     CDS             complement(3115..4095)
                     /gene="ribD"
                     /locus_tag="VF954_05205"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX9530513.1"
                     /translation="MRDEAGMERALALAASVRATAHPNPWVGCVLATPAGERFEGATQ
                     PPGGPHAEAVALAAAGDRARGATAYVTLEPCAHTGRTGPCADALIQAGVRRVVIGLED
                     PDSRVAGAGMERLRQAGVAVEAGVGSAAVADQLAPYLKHRRTGRPWVVLKLAASLDGR
                     IAAPDGTSRWITGAAARADGHRLRAESDVVIVGAGTVRRDDPALTARSATGPDPRRVV
                     LGAIPPGARVLPAEAAAGDLGALLDRLGSEGSVQVLVEGGAHVAHQFHAAGLVDRYVV
                     YLAPALFGGSDAVPLFEGPGVATAAELWRGRFVEVRQVGGDVRVELAGSC"
     regulatory      complement(4134..4261)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(4481..4960)
                     /locus_tag="VF954_05210"
     CDS             complement(4481..4960)
                     /locus_tag="VF954_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX9530514.1"
                     /translation="MNGPGLAAKVLTVSDGVVGGTREDRSGAALESRLAEAGYRIASR
                     RAITDGIESVRDGLVEMSVRFAGLIVTTGGTGFGPRDLTPEGTRAVLERDAPGLAEAM
                     RLANPLGRLSRGLAGTRGQSLILNTPGSPAGAVECLEAVLDVVPHALTLLAGRPAGH"
     gene            complement(4957..5631)
                     /gene="rpe"
                     /locus_tag="VF954_05215"
     CDS             complement(4957..5631)
                     /gene="rpe"
                     /locus_tag="VF954_05215"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX9530515.1"
                     /translation="MARIAPSILSADFAALGAAIDEVAPEADLLHVDVMDGHFVPNLS
                     IGPPVVRAIRRHTDLYLDCHLMMTNPGEYLEAFQAAGANGCTVHVEIGGTEDLIGHGR
                     ALGLDVGLAANPSTPFAAVAPYLDRIDLLLLMTVVPGFGGQRFMAEVLPKVREARAEI
                     DRQGLPVAIEVDGGIDCDTGPLAAEAGAGIFVAGSSIFGQPSPRQAAAGLRAAVDGSA
                     ARARTG"
     gene            complement(5644..>6680)
                     /locus_tag="VF954_05220"
     CDS             complement(5644..>6680)
                     /locus_tag="VF954_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX9530516.1"
                     /translation="DRGFATELVYGTTRMRRACDWLVDRHVPAGVDLEVRALLRLGAY
                     QLAFAGTPAHAAVSATVAEAPFKVRGLLNAVLRKVARALPPAWPDRATELSYPDWVVA
                     RLEADLGADRAVASMRSMNLPAVVHERPDGYVQDPSSQAVAGYVAGLVPAGGVVVELC
                     AAPGGKATALASSGAGLVVAGDVRPRRSALVAANSRRLALDRVAVVVADGRAPALRGA
                     SADVVLVDAPCSGLGALRRRPDARWRARPAAVNELAGLQRQLLGAAAALTRPGGALVY
                     SVCTLTAAETLDQDRWLAAAFPGLGALPPPSARGWDAGWEEAGRGGLLLPQAGDNDGM
                     YCLGLRAPCR"
BASE COUNT          879 a         2451 c         2474 g          876 t
ORIGIN      
        1 ggtccttgag ggcggggatc aggatgttga tctgggcctt cggcacgacg gcctcgaccg
       61 cgtagccgtc ggtgccgaag agcttcgaga cggtcggcga cttcatggag ggcagcaccc
      121 cgatgacgcc gtcgaggtcg gcctcggcca cgttgagctt gaccaggacc ttcgcccggg
      181 cctccagcgc cccagacaga agggtgtgga gctgggccat ggcgtggcgc ttctcggcgt
      241 caccggccgc gaccgggttg gcgatcagct ccgagtagga gctgaggatg gtgtcgatca
      301 cccgcagccc ggccgcccgc agggcccggc ccgtctcggt gaggtcgacc accacgtcgg
      361 cgatgtcggg gatcttggcc tcagtcgccc cgtacgacat gcggatgtcg gccgagatgc
      421 cgtgcttctc caggaacctg cgggtcagct cggggtactc ggtcgagacc cgcaacccct
      481 cggcgggcag gtccttgacc gtggcccacg gggagtcggc ggccacggcc agcacgatgt
      541 tggccgggcg ggccgtggcc ttggagtagt gcagctggcc gagggagacg acctcgctgc
      601 ccgtctcctc gatccagtcc cggcccgtga tgccgaggtc gaagaggccc tcggcgacgt
      661 agcgggggat ctcctggggg cgcaggatcc gcacgtaggc cacccgggga tcgtcgatgc
      721 tcgcccggta ctcgacctcc gagctgcgcg tcaccgccag gtcggccgcc tcgaacagct
      781 cgagggtcgc cttctccaac gagcccttcg ggagcacgag gcgcagcatc tcaggctccc
      841 tctttcccca cctgcggctt gggcagctgt cgcagcaggt ccgccatctc gatcgccgtc
      901 tcggccgcct cgtagccctt gttgcccgcc ttggtgccgg cccgctcgat ggcctgctcg
      961 acggtctctg tggtcagcac gccgaagacg accggcaccc cggtcgacag gccggccgcc
     1021 gccacccccg ccgcgcactg gccggcgacg agctcgtagt ggcccgtcgc gccccggatg
     1081 acggctccga gacagatgac cgcgtcgtac tcgccgctcg cggcgagccg ctgcgccacc
     1141 agcggcagct cgaacgcgcc gggcgcccag gcctcggtga tggacgccgg gtccacgcca
     1201 tggcgcagca gcccatcccg ggcgcccgcg aggagccgct cggtgatgaa gtcgttgaag
     1261 cgtgagcagg cgagggcgac ccgcagcccg tcgcctcgca tctggccttc gtagcggcgt
     1321 tcagaggacg tcatcgagtc cctccaggag gtgtcccatc cgctccctct tggtgcggag
     1381 ataggcgatg ttctcggggt tgggcacggt ctcgagggga acgcgctcca ccatctccag
     1441 cccgaagcct tccaggccac cgtacttggc cgggttgttg gtcatgtagc gcatcgtcgt
     1501 gatgccgagg tccaccagga tctgggcgcc gatgccgtac tcccggctgt cgacgggcag
     1561 gcccagctcg aggttggcgt ccacggtgtc gcggccctgt tcctggagcg tgtaggcccg
     1621 cagcttgtgg gcgatgccga tgccccggcc ctcgtggccg cgcaggtaca cgaccgcgcc
     1681 gcgtccctcg gtcgcgatga ggcggagggc ggcgtcgagc tgcgggccgc agtcgcagcg
     1741 cagcgagccg aagacgtctc ccgtcaggca ctcggagtgc acccgcacga gcacgttgtc
     1801 ctctccgtgg acgtcgcctt tgaccagggc caggtgctgg tcgcggttga tgacggactc
     1861 gtaggcgtag caggtgaagt cgccgtagcc gccgacgggg aggcgggcct cggcgatgcg
     1921 gcgcacgagc ttgtccttct gccggcgatg gcggatcagg tcggcgatgg agatcagcag
     1981 cagcccgtgc tcctcggcga agagctcgag ctcctccagc cgggccatgc ccgtcttgtc
     2041 gtgggtgacc acctcgcaca gcacgcccgc cgggtacagg cccgccgccc gggccaggtc
     2101 cacggccgcc tcggtgtggc cggcccgctt cagcacgccg cccgggcggt agcgcagcgg
     2161 caggatgtgg cccggtcggg acaggtcgtt gggccgggtg gcggcatcga tcagggaccg
     2221 gatggtcgac gcccggtcgg ccgccgagat gccggtcgtg gtgccgtggc gatagtccac
     2281 cgtgaccgtg aaggcggtgc gctgcgactc ggtgttggtc gacaccatga gcggcaggtc
     2341 gaggtcgtcg aggcgttcgc cggtgagcgg cacgcagatc aggcccgagg tgtgggcgag
     2401 gaagaaggcg atcttctccg gcgtggcgaa ctcggccgcc atgatgaggt cgccctcgtt
     2461 ctcccggtcc tcgtcgtcga ccacgaccac cacctcgcct cgggcgacgg cgtcgatggc
     2521 ggcctcgatc ttggcgaacg gcatgtcagc gctccctctc gaggaccagg cgctcgaggt
     2581 acttggccag gatgtcgact tcgaggttga cccggtcgcc ggggcagcga cgacccagcg
     2641 tggtgaccgc cgccgtgtgc gggatgacgg cgaccgcgaa cccgtcgtcg aggaccgacg
     2701 caacggtcag gctgcagccg tcgacggtga ccgagccctt ctcgaccagg tagcgcctca
     2761 agccctccgg agcgcggacc cgcaggtccg gggcgggctt caccacctcg cccacaccgt
     2821 ccacgtgacc ttgcacgatg tggccgccga ggcggtccgt gggtcggacc ggccgttcca
     2881 ggttgacccc gtcgccagcc cggaggtcgc ccaggttggt ccgggccagg gtctcgtcca
     2941 cggcgtcggc ctcccaccag ccctgcccga ccgccaccac cgtcaggcag cagccgttga
     3001 cggcgatcga gtcgccgggc gcggcgtcac cggcgaccag cggcccggcg aagcggaacc
     3061 gggccccgtc ccggccctcc aggcagccca gctcctccac gatgccggtg aacatcagca
     3121 gctcccggcc agctcgaccc gcacgtcgcc ccccacctgc cggacctcga cgaaccgccc
     3181 ccgccacagc tcggccgccg tggccacccc gggcccctcg aacagcggca cggcgtcgga
     3241 gccgccgaaa agcgccgggg ccaggtagac cacgtagcgg tcgacgaggc cggcggcgtg
     3301 gaactggtgg gccacgtggg cgcccccttc caccaggacc tgcaccgagc cctcggaccc
     3361 cagccggtcg agcagggcgc ccaggtcccc ggcggcggcc tcggccggca gcacgcgcgc
     3421 cccgggtggg atggcgccga gcaccacgcg acggggatcc gggccggtgg ccgagcgggc
     3481 ggtgagggcc gggtcgtcgc ggcgcacggt ccccgccccg acgatgacga cgtcgctctc
     3541 ggcccggagc cggtggccgt cggcccgggc ggccgcaccg gttatccacc tgctcgtccc
     3601 gtccggcgcg gcgatgcgcc cgtcgaggct ggcggccagc ttgagcacga cccacggccg
     3661 gccggtgcgg cggtgcttga ggtagggcgc cagctgatcg gccaccgccg ccgaacccac
     3721 gccggcctcc acggcgacgc ccgcctggcg cagccgctcc atgccggcgc cggcgacccg
     3781 ggagtcgggg tcctcgaggc cgatcaccac ccggcgcacc ccggcctgga tcagggcatc
     3841 ggcgcagggc ccggtgcggc cggtgtgggc gcacggctcg agggtgacgt acgcggtggc
     3901 gccccgggcc cggtcgcccg cggccgccag ggcgacggcc tcggcgtggg gcccgcccgg
     3961 gggttgggtg gccccctcga agcgctcgcc ggcgggagtg gcgaggacgc agcccaccca
     4021 cgggttgggg tgggcggtgg cccgcacgga ggcggccagg gccagggctc gctccatgcc
     4081 tgcctcgtcc cgcatcgcca agctccgttc cggcgcgcgc ggcgaaaccg ttcgcgccct
     4141 cgctcccatc cggactgtca ccgtcggccc cggggttcca ccgggtcggc ccccggcgtg
     4201 cgccgggggt tcgcgggctc tcaccgccgg tcggggattg cacccaaccc cgcgaggggc
     4261 tcttgtcctg ctcttggttg tatgagttgt ggtcgtatga ctggttcttg accactcagt
     4321 ctaccgggcg cgctggtcgc tttccatccg atcggatgga aagcgaccgc tagcgaccgc
     4381 tcgcggccgg ggacacgctg aaaatcattc ttccagcaaa aaagtcgcta taacgacatt
     4441 gaagcatgga agaatgggcg gcacacccgc cgctggcctg ctagtggccg gcgggacggc
     4501 cggcgaggag ggtgagggcg tggggcacca cgtcgagcac ggcctccagg cactcgacgg
     4561 cgcccgccgg ggagcccggt gtgttgagga tgagcgactg ccccctggtc cctgccaggc
     4621 cgcgcgacag gcgccccagg gggttggcga gccgcatcgc ctcggccagc ccgggcgcat
     4681 cccgctcgag gacggcgcgc gtcccctccg gggtgaggtc ccggggcccg aacccggtgc
     4741 cgcccgtggt cacgatgagg cccgcgaacc tgacgctcat ctcgaccagc ccgtcacgca
     4801 ccgactcgat gccgtcggtg atcgcccgcc gggacgcgat gcggtagccg gcctcggcca
     4861 gcctgctctc cagggcggcg cccgagcggt cctcccgggt gccccccacg acgccgtcgg
     4921 agacggtgag caccttggcg gccaggccgg gcccgttcat ccggtgcggg cccgggcggc
     4981 ggacccgtcc accgcggcgc gcaggcccgc ggccgcctgg cgcggggagg gctggccgaa
     5041 gatcgacgag ccggccacga agatgcccgc cccggcctcg gccgcgagcg gcccggtgtc
     5101 gcagtcgatg ccgccgtcca cctcgatggc gacgggaagg ccctgccggt cgatctcggc
     5161 gcgggcctcc cgcaccttcg gcagcacctc ggccatgaag cgctgcccgc cgaagcctgg
     5221 caccaccgtc atcagcagca gcaggtcgat ccggtcgagg tagggggcca cggccgcgaa
     5281 gggcgtggac gggttggcgg ccagcccgac gtcgaggccg agggcccgcc catggccgat
     5341 gaggtcctcg gtgcccccga tctcgacgtg caccgtgcac ccgttggccc ccgccgcctg
     5401 gaacgcctcg aggtactccc ccgggttggt catcatcagg tggcagtcca ggtagagatc
     5461 ggtgtggcgc cggatggccc gaaccacggg cggtccgatg ctgaggttgg gcacgaagtg
     5521 gccgtccatg acgtcgacgt gcaggaggtc ggcctcgggc gcgacctcgt cgatggcggc
     5581 gcccagggcg gcgaagtccg ccgacaggat cgagggcgcg atgcgggcca tcgacgggcg
     5641 agcctatcgg cagggggcgc gcagaccgag gcagtacatg ccgtcgttgt ctccggcctg
     5701 gggcagcagg agcccacccc gccccgcctc ctcccacccg gcgtcccacc cccgggcgct
     5761 gggtggtggc agggcgccca gcccggggaa ggcggccgcc agccaccggt cctggtcgag
     5821 ggtctcggcc gcggtgaggg tgcacacgct gtagaccaac gctccgcccg ggcgggtcag
     5881 ggccgccgcc gccccgagca gctggcgctg gagccctgcc agctcgttga ccgcggcggg
     5941 ccgcgcccgc caccgggcgt cgggccggcg gcggagggca cccagcccgg agcagggcgc
     6001 gtcgaccagc accacgtcgg ccgaggcgcc acgcagggcc ggggcccggc cgtcggccac
     6061 cacgaccgcg acccggtcga gggcgaggcg gcgggagttg gcggccacca gggcggaccg
     6121 gcggggtcgc acgtcgccag ccaccaccag cccggctccg gaggacgcca gcgcggtggc
     6181 cttgccgccg ggggcggcgc agagctcgac caccactccg ccggcgggca ccaggccggc
     6241 gacgtagccg gccacggcct gggaggacgg gtcctggacg tagccgtcgg ggcgttcgtg
     6301 gaccacggcc ggcaggttca tgctcctcat ggaggcgacg gcccggtccg cgcccaggtc
     6361 ggcctccagc cgggccacga cccagtccgg gtagctgagc tcggtggccc ggtcgggcca
     6421 cgccggcggg agggcccggg ccaccttgcg gagcaccgcg ttgagcaggc cccgcacctt
     6481 gaagggcgcc tcggccacgg tggccgacac cgcggcatgg gcgggcgtgc cggcgaaggc
     6541 cagctggtag gcgcccagcc ggagcagggc gcgcacctcg aggtcgacgc cggccgggac
     6601 gtggcggtcg accagccagt cgcacgcccg ccgcatgcgg gtggtcccgt agaccagctc
     6661 ggtcgcgaag cctcgatccc
//
LOCUS       DASWGG010000289         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3165912, whole genome shotgun sequence.
ACCESSION   DASWGG010000289 DASWGG010000000
VERSION     DASWGG010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3165912"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VF954_05225"
     CDS             complement(<1..1118)
                     /locus_tag="VF954_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9530517.1"
                     /translation="MTALDAVNRTVDSGAARAAAVIAAVLLLVAASCQGGALTGADAI
                     PTFRVGTAQPASLDPVLASQPQETLVARQLFDGLVRYDDTTAAVAPDVATSWEIDATS
                     TVFTFHLRARSRFSDGEYVTAASFVRGMTRALTPAVYRAPGSLGYELDGIAGAADVVS
                     GRSPTLSGARALDPLTLEIRLSAPDAQFLVRCGDVAFSPLPSAAAVAARQPSWARFPL
                     GNGPFKLSRPMVANQAIVLTPNALHAGGHPKVAQVALEIYNDVAASYAAWRAGRLDWS
                     AYPPDRMAEVRRHYRSSSVIRPTAGLDGLVLDEGSAPASNPLFRQAVSLAVDRAAIAR
                     GVLANAVLPAAGLVPALVPGAAAGGGASPCPSCGFDPAR"
     gene            complement(1200..>2090)
                     /locus_tag="VF954_05230"
     CDS             complement(1200..>2090)
                     /locus_tag="VF954_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX9530518.1"
                     /translation="AGESAAVSAARLDAILLGPLREDLGDGPLVLVPTGALHALPWGL
                     LATCRGRPVSVAPSATLWHRASARRAPAGPSAPAGRAAPGASSVPADPSDPAGRAGRA
                     AASVLAGGQGRVVLVAGPGLPGAAAEVAALRRRYPGATSFTPRRARVEDVLSALDGAS
                     LAHIAAHGRFRSDNPLFSSLALGDGPLMVYDIERMRRAPRRIVLSACDSGVSGVRPGD
                     ELMGLAAALFAQEAATLVASVVPVSDEATRPLMVAFHKALQDTGGPAAALARVAASQE
                     GADPAAYAASAAFVCFGAGW"
BASE COUNT          296 a          766 c          820 g          208 t
ORIGIN      
        1 cgggcggggt cgaagccaca cgacgggcac ggcgacgccc ccccaccggc ggcggcgccg
       61 gggaccagcg ctgggacgag cccggccgcc gggagcacgg cgttggcaag gacccccctg
      121 gcgatggccg cccggtccac ggcgagggag acggcctggc ggaagagcgg gttgctcgcc
      181 ggcgccgacc cctcgtcgag gaccaggccg tcgaggccgg ccgtcggacg gatgaccgaa
      241 ctggaacggt agtggcgcct gacctccgcc atcctgtccg gtgggtacgc cgaccagtcg
      301 agtctgcccg cccgccaggc ggcgtacgac gctgcgacgt cgttgtagat ctccagcgcc
      361 acctgggcca ccttggggtg acccccggcg tggagggcgt tgggcgtcag cacgatggcc
      421 tggttggcca ccatcggccg gctcagcttg aacggcccgt tgccgagcgg gaaccgggcc
      481 caggagggct ggcgggccgc gaccgcggcg gcgctcggca gcggcgagaa tgcgacgtcg
      541 ccgcagcgca cgaggaactg ggcatccggt gcggacagcc ggatctccag cgtcagggga
      601 tcgagggccc gggcacccga cagcgtcggg gagcgcccgc tgacgacgtc ggcggcaccg
      661 gcgatcccgt cgagctcgta gccgagcgac ccgggagcgc ggtagacggc gggcgtcagc
      721 gccctcgtca tcccccggac gaagctggcc gccgtcacgt actcgccgtc cgagaagcgg
      781 gacctcgccc ggaggtggaa cgtgaagacg gtcgaggtgg cgtcgatctc ccacgaggtg
      841 gcgacgtcgg gcgcgacggc ggccgtggtg tcgtcgtagc gcacgagacc gtcgaagagt
      901 tgccgggcca ccagcgtctc ctgcggctgg gacgccagga cgggatcgag ggaggcgggc
      961 tgcgccgtgc cgacccggaa cgtcgggatg gcatccgccc cggtgagggc gccaccctgg
     1021 caggaggcgg cgaccaggag cagcacggcg gcgatcaccg ccgcggcgcg ggccgctccg
     1081 gaatccactg ttcggttcac cgcgtccagc gcggtcacac gaacactcgg ttgggaaacg
     1141 gccccgggct cctggtcacc ggggcatcct agtggtggtt ctggccccgg ctggggcggc
     1201 taccagcccg ccccgaagca cacgaacgcc gccgacgccg cataggcggc cgggtcggca
     1261 ccctcctggg acgccgccac ccgggcgagg gccgccgcgg ggccgcccgt gtcctgcagc
     1321 gccttgtgga aggccaccat gaggggccgg gtcgcctcgt cggagacggg cacgacactc
     1381 gcgacgaggg tagccgcctc ctgggcgaac agcgcggcgg cgaggcccat cagttcgtcg
     1441 ccgggccgga cgccggacac gccggagtcg caggcggaga ggacgatccg ccgtggcgcc
     1501 cggcgcatac gctcgatgtc gtagaccatc agcggcccgt ccccgagggc gagcgaggag
     1561 aacagcgggt tgtcgctccg gaaccgcccg tgggcggcga tgtgggcgag agaggcgccg
     1621 tcgagcgccg agaggacgtc ctccacccgg gctcggcggg gcgtgaaact cgtggccccc
     1681 gggtaccgcc ggcgcagggc ggcgacctcg gccgcagccc cggggagtcc cggcccggcg
     1741 acgagcacca cccgcccctg accccccgcc aacacggagg cagccgctcg cccggccctg
     1801 cccgctgggt cggaagggtc cgccggcacg gaagaggccc ccggcgcggc ccggcccgcc
     1861 ggcgcggagg ggcccgccgg cgctcgccgc gcggaagccc ggtgccacag ggtcgccgac
     1921 ggggcgacac tcaccggccg tccccggcac gtcgccagca gaccccacgg cagggcgtgc
     1981 agggcgccgg tcggcaccag gaccaggggc ccatcgccga ggtcctcccg cagcggaccg
     2041 agcagtatgg cgtcgagccg ggcggcggag acggcggcgg actcccccgc
//
LOCUS       DASWGG010000290         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3167185, whole genome shotgun sequence.
ACCESSION   DASWGG010000290 DASWGG010000000
VERSION     DASWGG010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3167185"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VF954_05235"
     CDS             <1..550
                     /locus_tag="VF954_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX9530519.1"
                     /translation="RSSPARAEIVVHVDWPALVRGYAQGDERCELVGVGPVPVSLVRD
                     LANDCWLKVIVTNGVDVMAVSHGGRNVPAHIRSALEARDPTCVVPGCHVRAGLEIDHW
                     KIPYHQGGPTELWNLCRMCRAHHFDKTHRGARIEGGPSHWVWHPPPSAGSPSRTTPTA
                     PAGGRAWPQPLAFDQLRHRPPE"
     gene            562..1833
                     /locus_tag="VF954_05240"
     CDS             562..1833
                     /locus_tag="VF954_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530520.1"
                     /translation="MGSIVVLSTTAVTVEPGGTATVGVKITNTGQVVDEFTIGVVGEA
                     AAWATAEPATVSLFPNAQGEATVRFHPPRSADSTAGAVAFGVRVASKEDAAGSRVEEG
                     TLTVSPFRDVFVELVPRTSRGRRGAKHSFRVDNRGNGPLVASVVASDPDSLLRFRPKP
                     AALDLGPGLAAFVSVEVRPRARFWRGPAQLKPFQVWAQEEGQPPVLSDGSFLQEPVLP
                     PWLVKAALVVAALAIVLFVLWQTVLKPKIKSLAKVEATNAVAAQLGTTPAAAAAAAKT
                     KAGGGALTPAGGAPSSGSGSSGTGTSGSASTGTGTAAPAVATTKQPIDGRLFLTASGT
                     APYVVPTGKQLQLTDIVLENPQGNTGQLLIQRSGTPLLVTELADFRDLDYHFVSPIVF
                     GPGQKLELVANCTSPTCTPGAYFSGSLVDAS"
     gene            complement(2120..2371)
                     /locus_tag="VF954_05245"
     CDS             complement(2120..2371)
                     /locus_tag="VF954_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530521.1"
                     /translation="MVELVAVVDVVAVTPGLVVVVANVVDVVVVGAEVVEVVVVGGGH
                     GAVKVVADRVDATGPLPLSQYAATTWLPGPVALNVAVPL"
BASE COUNT          359 a          969 c          850 g          358 t
ORIGIN      
        1 gcggtcctcg ccggcccggg ccgagatcgt ggtgcacgtc gactggccgg cgctggtgcg
       61 cggctatgcc cagggcgacg agcgctgcga gctggtcggg gtgggaccag tgccggtgtc
      121 gctggtgcga gacctggcca atgattgctg gctcaaggtc atcgttacca acggcgtgga
      181 cgtgatggcg gtcagccacg gcgggcgcaa cgttccggcc cacatacgct ccgccctcga
      241 agcccgcgat cccacctgcg tggtgccggg ctgccacgtg cgggcgggcc tggagatcga
      301 ccactggaag atcccctacc accagggtgg gcccaccgag ctgtggaact tgtgccggat
      361 gtgcagagcc caccatttcg acaagaccca ccggggcgcc cgcatcgagg gcggtcccag
      421 ccattgggtg tggcaccccc cacccagcgc cggctcgcct tccaggacaa caccaaccgc
      481 gccagccggc ggccgggcct ggccacaacc gctcgccttc gatcaactcc gccatcggcc
      541 gcccgagtag ccttgcatgg cgtgggttcc atcgttgtgc tttcgaccac ggccgtgacc
      601 gttgaacccg gcgggacggc gaccgtcggc gtgaagatca ccaacaccgg gcaggtggtg
      661 gacgagttca ccatcggggt ggtgggcgag gcggcggcct gggccacggc cgagccggcc
      721 acggtctccc tgttccccaa cgcccagggc gaggccaccg tccgcttcca cccgccccgg
      781 tccgccgaca gcaccgccgg cgcggtggcc ttcggtgtcc gggtggcgtc caaggaggac
      841 gcggccggct cgcgggtcga ggagggcacg ctcaccgtgt ctcccttccg cgacgtcttc
      901 gtcgagctgg tgccccggac gagccggggc cgccgcggcg ccaagcactc cttccgcgtc
      961 gacaaccggg gcaacgggcc gctggtcgcc agcgtggtcg cctcggaccc cgacagcctg
     1021 ctgcggttcc ggcccaagcc cgccgccctc gacctcgggc ccggtctggc cgccttcgtc
     1081 tccgtggagg tccggcccag ggcccggttc tggcgcgggc cggcgcagct gaagcccttc
     1141 caggtctggg cccaggagga gggccagccc cccgttctgt cggacggctc cttcctccag
     1201 gagccggtcc tgccgccatg gctcgtgaag gccgctctcg tggtggcggc gttggcgatc
     1261 gtgctgttcg tgctgtggca gacggtcctc aagcccaaga tcaagtccct ggccaaggtc
     1321 gaggccacga acgccgtggc cgcccagctg ggcacgacac cggccgcggc cgcggctgca
     1381 gccaagacca aagccggggg cggcgccctc acgcccgccg gcggggctcc gtcgtccggg
     1441 agcgggtcgt caggcaccgg gacttccggt tcggcgtcga cgggtaccgg cacggcggcg
     1501 ccggccgtgg cgacgacgaa gcagccgatc gacggtcgtc tgttcctcac cgcttccggc
     1561 acggcgccct acgtcgtccc caccggcaag cagctgcagc tcaccgacat cgtcctcgag
     1621 aacccccagg gcaacaccgg ccagctgctc atccagcgca gcggcactcc cctgctcgtg
     1681 accgagctgg ccgacttccg ggacctcgac taccacttcg tctcccccat cgtcttcggg
     1741 cccggccaga agctcgagct ggtggccaac tgcacgagcc cgacgtgcac ccccggcgcg
     1801 tacttctccg ggtccctcgt cgacgcatcc tgatgcgact gcgggccact tccgcaccgg
     1861 cgcgcatcct cgcggccgca ctggtcgccg gggtcgtggc cctgccctcg gtggcgcccg
     1921 cggcgaccgc cccgcccccg ccaccaacaa cgatcatgat ctcgccgaat tgcggcgatg
     1981 cagccgcttc gacctggaca ttcactgcat ccggttctgc cccgtcagcg cctgtcggtg
     2041 gtttcagcga cgtggacatc gtccttgacg acggagctcc gaactcccag aggacccaga
     2101 tcagatactc gggtacgagc tacagcggta ccgcaacgtt caaggccact gggcccggca
     2161 gccatgtcgt ggccgcatac tgggacaacg gcaacggtcc ggtcgcgtcc acgcgatcgg
     2221 caaccacctt cacggcgccg tgtccgcctc cgaccacgac cacctcgacc acctccgcgc
     2281 ccacgaccac gacgtccacc acgttggcca cgaccaccac caagccgggg gtgacggcga
     2341 ccacgtcgac cacggccacg agttcgacca cctcgacgac cgcggcggcg ccggcccacc
     2401 tggcgaccga cccggcgctg ggacctccgg ggacggtggt acgggtgacc ggggcgggct
     2461 ttcaccccgg gccggtcacg ctggcgtggt cacccggtga gggaggggcg accgcgatcg
     2521 cggacgccgc cggcac
//
LOCUS       DASWGG010000291         4418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1240223, whole genome shotgun sequence.
ACCESSION   DASWGG010000291 DASWGG010000000
VERSION     DASWGG010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240223"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VF954_05250"
     CDS             <1..466
                     /locus_tag="VF954_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530522.1"
                     /translation="AEEAGVALDPAALVPLSHWTPPPTAPRRFTTWFFLTRWPEGATV
                     EVDGAEIHAHRWLSPADALRRRDEGELSLAVPTCVTLWGLAGAQNVAVALGRAERADP
                     ARYTTHLVHGEAGPVALWFGDAAYDDGDLSRPGGRHRLVMGRTPWRYEGPVE"
     gene            528..1298
                     /locus_tag="VF954_05255"
     CDS             528..1298
                     /locus_tag="VF954_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9530523.1"
                     /translation="MPGPLADTAASRSATLTSAARVVDGVKIYGRGETEVRALDCITT
                     DFTRGAFSAIMGPSGSGKSTLMHCLAGLDRLTSGSVIIGGTEIGMLNDRALTALRRDR
                     IGFVFQTYNLVPTLTAVENITLPLDLAGRKPDPAWLDAIVTTLAIGDRLKHKPAELSG
                     GQQQRVAVGRALVARPEIIFADEPTGNLDSHASAEVLGLLKDAVARLGQTVVMVTHEP
                     VAAAYAERVIFLADGRIGDEMTSPTAEAVLDRMKDMGA"
     gene            1303..3837
                     /locus_tag="VF954_05260"
     CDS             1303..3837
                     /locus_tag="VF954_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9530524.1"
                     /translation="MLRATLKNLAAHKLRLALTGLAVVLGVSFMAGTFVLTDTIKRTF
                     DNLFTQTSAGKDVIVRGATAFTGGRGPEGAFGGNRPLVPASVLATVRSTPGVQFADGS
                     VQGQATLLATNGKAIVKHGPPTLAFAWLPDRQLSSFTLRSGRGPSAPDELTVDSGTVK
                     DQHFHLGQRLTVVSNQPPRQFTLVGVTGFGAADNVAGATLLAFTPGTAQELVGKTGYF
                     SEIDVARRSDSTVDALQSTIAGRLPANMEAVTATSVAQQAASAVATAANLFNKVLLSF
                     ALIALFVGAFLIFNTFSILVGQRTRELALLRAVGASRGQVNRSVLTEALVVGLGGSLV
                     GVGLSLPLSAGLYGLLRSVGFGLPSSGLRLLPRTVVVSMLTGTAITMFSAILPARRAS
                     RVPPVAAMREDVLEPETSLRRRALIGGAVLAVGLVLLFGGLFLGFGIALVGAGCAVTF
                     VGVAMLTPFISAPLARLIGAPLVRAGALPSRLARENSARNPRRTAGTASALMIGLAVV
                     TAVATIGSSARASFSAQFDRSIRADYVVTSSREGFPGAVEPVVRHAPGVTAFSPVRNA
                     SWHDGKAGKDLTGISPTDGPGLVALHMVTGSAAALGAGRLLVDDKVARSRHLSLGQTV
                     EMTFGATGTVPIIIGGTYASNQFLGNYLASDDFVAANVNQVQDVAILVDTSAASPAEE
                     QKLNDAIAPYGNVKAQTGAQFKNQQKGQLDTALLAVYLLLALSILIALIGVVNTLALS
                     VMERTREIGLLRGIGMQRRQTKRMIRGEAVVVSLIGAVLGLALGVGLGAAVVRAIGTG
                     TISTIVVPVSTIVVVVVLTGIFGVVASWLPARRAARLDVLQAIATT"
     gene            3870..>4418
                     /locus_tag="VF954_05265"
     CDS             3870..>4418
                     /locus_tag="VF954_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9530525.1"
                     /translation="MADIHVPDIDLNDIELWSGPLSVRDDAFARLRAEQPIAWMGVPG
                     YAFGEAGGYWSLTRHAHVLEASKRPEDFCSGRGTNLFDMPPDFLEFFGSMINMDDPKH
                     ARLRRIVSRGFTARKLDEARESVERAAAEIVEAIAARGECDFVTDVAALLPLRIIMDM
                     LGIPRSYEELILERTNRIVGGSD"
BASE COUNT          591 a         1666 c         1505 g          656 t
ORIGIN      
        1 ggccgaggag gcgggcgtcg cgctggaccc ggccgcgctg gtcccgctct cgcactggac
       61 cccgcccccc accgcaccgc ggcggttcac gacgtggttc ttcctgaccc gctggcccga
      121 gggcgccacc gtggaggtag acggcgcgga gatccacgcc caccggtggt tgagccccgc
      181 cgacgcgctc cgccgccgcg acgaaggcga gctcagcctg gccgtgccga cctgcgtcac
      241 gctctggggg ctggccggag cccagaacgt cgccgtcgcc ctgggccgag ccgaacgggc
      301 cgacccggcg cggtacacga cccacctggt gcacggcgag gccgggccgg tggcgctgtg
      361 gttcggggac gccgcctacg acgacggcga cctgagtcgc cccggtgggc gccaccggct
      421 cgtcatgggc cgcactccgt ggcgctacga gggcccggtc gaatagcgca tcccacggtg
      481 aacggacccg acccgcggcg accggtccga ggaggcaaag tggaggcatg ccaggtccac
      541 tcgccgatac cgccgcgtcc cgttcggcca ccctgacctc ggcggcccgg gtcgtcgacg
      601 gggtcaagat ctacggtcgg ggcgagacgg aggtccgtgc gctcgactgc atcacgaccg
      661 acttcacccg gggcgccttc agcgccatca tgggcccgtc cggctcgggc aagtcgaccc
      721 tgatgcactg cctggccggc ttggaccgcc tgacgagcgg gtcggtgatc atcggcggca
      781 ccgagatcgg catgctcaac gaccgggccc tcacggcgct gcggcgggac cgcatcggct
      841 tcgtcttcca gacctacaac ctggtgccga ccctgaccgc agtggagaac atcaccctgc
      901 ccctcgacct cgccggccgc aagccggacc cggcctggct cgacgccatc gtgaccacgc
      961 ttgccatcgg cgaccggctc aagcacaagc ccgcggagct ctccgggggc cagcagcagc
     1021 gggtggccgt ggggcgggcg ctggtggccc ggcccgagat catcttcgcc gacgagccca
     1081 ccggcaacct ggactcgcat gccagcgccg aggtcctcgg cctgctcaag gatgcggtcg
     1141 cccggctcgg ccagacggtg gtcatggtga cccacgagcc ggtggcggcc gcctacgccg
     1201 agcgggtgat cttcctcgca gacggccgga tcggcgacga gatgacctcc ccgacggccg
     1261 aggccgtgct cgaccgcatg aaggacatgg gggcctgatc aggtgttgag ggcgaccctc
     1321 aagaacctgg ccgcccacaa gctgcggctg gccctcaccg gtcttgcagt cgtgctcggc
     1381 gtgtccttca tggccgggac cttcgtgctg accgacacca tcaagcgcac cttcgacaac
     1441 ctgttcaccc agacctcggc cggcaaggac gtcatcgtgc gcggggccac ggccttcacc
     1501 ggcgggcggg ggccggaggg cgccttcggc ggcaatcgcc cgctcgtccc cgcgtccgtg
     1561 ctcgccaccg tccggtccac cccgggggtc cagttcgcgg acggctccgt ccagggccag
     1621 gccaccttgc tcgccaccaa cggcaaggcg atcgtcaagc acggcccgcc gacgctggcc
     1681 ttcgcctggt tgccggaccg gcagctgtcc tcgttcaccc ttcgctcggg ccggggcccc
     1741 tcggcccccg acgagctcac cgtcgactcg ggcaccgtca aggaccagca cttccacctg
     1801 ggccagcgcc tcaccgtggt gagcaaccag ccgccgcgcc agttcacgct cgtgggcgtc
     1861 accgggttcg gcgcggccga caacgtcgcc ggcgccacgt tgctcgcctt cacgcccggc
     1921 acggcccagg agctggtcgg caagaccggc tacttcagcg agatcgacgt ggctcgccgg
     1981 agcgactcga ccgtcgacgc cctccagtcg acgatcgcgg gccgcctccc cgccaacatg
     2041 gaggccgtca ccgcgaccag cgtcgcccag caggccgcca gcgcggtggc caccgcggcc
     2101 aacctcttca acaaggtgtt gctcagcttc gccctcatcg cccttttcgt cggggccttc
     2161 ctgatcttca acaccttcag catcctggtc ggccagcgca cccgggagct ggcgctgctg
     2221 cgggccgtcg gcgccagccg cggccaggtg aaccggtcgg tgctgaccga ggcgctggtc
     2281 gtcggcctgg gcggctcgct cgtcggcgtc ggcctgagcc tgcccctgtc ggccgggctg
     2341 tacggcctgc tgcgcagcgt cgggttcggg ttgccatcgt cgggcctgcg cctcctgccc
     2401 cggaccgtcg tcgtgtcgat gctgacgggc acggccatca ccatgttctc tgccatcctc
     2461 ccggcgcgcc gggcctcccg ggtcccgccg gtggcggcca tgcgcgagga cgtgctggag
     2521 ccggagacct cgctgcgccg gcgggccctg atcggcggtg cggtgcttgc cgtcgggctg
     2581 gtgttgctgt tcggcggcct cttcctcggc ttcggcatcg ccctggtcgg cgccggctgc
     2641 gcggtgacgt tcgtcggggt ggccatgctc acgcccttca tcagcgcgcc cctggcccgg
     2701 ctcatcggcg caccgctcgt ccgggccggc gccctcccct cccggctggc gagggagaac
     2761 tcggcccgca acccccggcg caccgccggg accgcctcgg cactaatgat cggcctggcc
     2821 gtggtcaccg cggtggccac catcgggtcg tcggccaggg cgtcgttctc ggcccagttc
     2881 gaccgcagca tccgggccga ctacgtggtg acgtcttccc gggaaggctt tccgggcgcg
     2941 gtggagcccg tcgtgcggca cgcgcccggc gtcaccgcct tcagcccggt gcgcaacgcc
     3001 agttggcacg acggcaaggc gggcaaggac ctgaccggga tcagcccgac cgacgggccc
     3061 ggcctggtcg cgctccacat ggtgaccggc tcggcggccg ccctcggggc cggccggctg
     3121 ctcgtcgacg acaaggtggc ccggagccgc cacctctcgt tgggccagac ggtcgagatg
     3181 accttcggcg ccaccggcac cgtcccgatc atcatcgggg ggacctacgc gtccaaccag
     3241 ttcctgggga actacctggc ctctgacgac ttcgtcgccg ccaacgtcaa ccaggtccag
     3301 gacgtggcca tcctggtcga cacctcggcg gcttccccgg ccgaggagca gaagctcaac
     3361 gatgcgatcg ccccatatgg caacgtgaag gcgcagaccg gcgcgcagtt caagaaccag
     3421 cagaagggcc agctcgacac cgccctcttg gcggtatatc tcctgctggc cctgtccatc
     3481 ctcatcgccc tcatcggcgt ggtgaacacc ctcgccctgt cggtcatgga gcggacccgc
     3541 gagatcgggc tcctgcgggg catcgggatg cagcgccgcc agaccaagcg catgatccgg
     3601 ggcgaggccg tggtcgtctc cctcatcggg gcggtgctcg gcctggccct cggcgtgggc
     3661 ctcggggccg cggtggtccg cgccatcggc accggcacca tctcgaccat tgtcgtgccg
     3721 gtctccacca tcgtcgtcgt ggtggtcctc accggcatct tcggggtggt cgcgtcctgg
     3781 ttgccggcgc gccgggccgc gcggctggac gtgctccagg ccatcgccac gacctgaccc
     3841 acgccccgca ggcgaactac cctcggcccg tggcagacat acacgttccc gacatcgacc
     3901 tgaacgacat cgagctctgg agcgggccac tgtcggtgcg agacgacgcc ttcgcccgcc
     3961 tccgggccga gcagcccatc gcgtggatgg gcgttcccgg ttatgccttc ggcgaggcgg
     4021 gcggctactg gtcgctgacc aggcacgcgc acgtgctgga agccagcaaa cggcccgagg
     4081 acttctgctc gggccggggc accaacctgt tcgacatgcc gccggacttc ctggagttct
     4141 tcgggtcgat gatcaacatg gacgatccca agcacgcccg gctccgccgg atcgtctcgc
     4201 ggggcttcac cgcccgcaag ctggacgagg cccgcgagtc ggtggaacgg gcggcggccg
     4261 agatcgtcga ggccatcgcg gcgcggggcg agtgcgactt cgtgaccgac gtggccgccc
     4321 tgcttccgct gcggatcatc atggacatgc ttgggatccc tcgcagctac gaggagctca
     4381 tcctcgagcg gaccaaccgc atcgtcggcg gcagcgac
//
LOCUS       DASWGG010000292         3623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_911394, whole genome shotgun sequence.
ACCESSION   DASWGG010000292 DASWGG010000000
VERSION     DASWGG010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911394"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VF954_05270"
     CDS             complement(<1..391)
                     /locus_tag="VF954_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX9530526.1"
                     /translation="MRTVEGAAAGQAARVAFPVFGTGAVVLVADPDRAAAARAAVDTV
                     LDRVDRSCSRFRDDSELSALNRANGRPVPASATFMDAVEVALRGARLSDGTVDPTVGQ
                     AVRLIGYDRDFRSVHPDDPRPVARVAPV"
     gene            complement(391..693)
                     /locus_tag="VF954_05275"
     CDS             complement(391..693)
                     /locus_tag="VF954_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530527.1"
                     /translation="MPSPADRDLGLRRLARATRWVAAGSVALTGLLAGVAAQAFAGHP
                     AAATTSAGTSSSANATTTTTTGTGLASSSSADDGLQQPASVPQATTRRGRVTSGGS"
     gene            818..1804
                     /locus_tag="VF954_05280"
     CDS             818..1804
                     /locus_tag="VF954_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530528.1"
                     /translation="MDEQFVHQVPEPLPRVATWHEHWRESLFFIMHRPDGPGDVLILT
                     LAHFPARQEMDALQLGRVGGQPVFARVARPYGDDPHTMAVGPVRIDIAEPYRTVHLHV
                     DEDPAAAVALELTFAARTRAHGLRRGTMKWRHETVWDQSHMIQSGTFNGTVSRGGETF
                     EIDNWWGQRDHSWGIRDHARCPLWIWLAIQFPEGMLGVWHWEYPNGARVYTDGCFAPA
                     GDADPVPVVDFQHDLRWLDAAGAEVSYGRDGEKVTGLAGQVVFTLEGGRRITVDAEGR
                     WAQRYGPLGGGLSEMAVRTSDGWQGTAIYEVTGAFHHRYFPEARAERLPPGE"
     gene            1904..2290
                     /locus_tag="VF954_05285"
     CDS             1904..2290
                     /locus_tag="VF954_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX9530529.1"
                     /translation="MPHPFLSSTWFDEMDKLRAEAPEPTGATADLTVNIVVTGAPEGD
                     AEVHIKGGQFDRGLLEGAPTKLTVPYAVAKSIFIDGNQQAGMQAFMSGQIKVEGDMTK
                     LMAMQAGGTPSAEQQAFQQKLKDLTE"
     gene            complement(2320..2964)
                     /locus_tag="VF954_05290"
     CDS             complement(2320..2964)
                     /locus_tag="VF954_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530530.1"
                     /translation="MAVRSATGRPRRPALRAAAVAAAVAVAGPGAAVTACGSHPHYSG
                     VAPKKWGQTVCEAWNAWQQDTASRLAAFRTKTATLTLAADQRDQLVGLFDALGQTSAQ
                     MVTTVRDAGHPAVKEGPAAEDAYVSTLAAFQTTYSDAKTRAAGLPLDDNAKYTQDVQA
                     ISAMLDSALKQITARISQIGRTYRDPKLTSALGQPACRSLSLPAATTTTTTPGP"
     gene            3063..3386
                     /locus_tag="VF954_05295"
     CDS             3063..3386
                     /locus_tag="VF954_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530531.1"
                     /translation="MRVPGDERLTARSGRGGPAPVTVSAWEAHLGRRSYVPWQCGDCE
                     AVLPFPKEAEVACPRCGQGAEASARLASRLESAAGAASDPATRAWLLHTASAWRGLPG
                     RTPAG"
BASE COUNT          470 a         1289 c         1381 g          483 t
ORIGIN      
        1 ccacaggcgc gacgcgcgcg accggccgcg gatcgtccgg atggaccgag cggaaatcgc
       61 ggtcgtagcc gatcagccgg accgcctggc cgacggtcgg gtcgacggtc ccgtcgctga
      121 gccgggcgcc cctgagagcg acctcgacgg cgtccatgaa ggtggcgctg gccggcacgg
      181 gacggccgtt cgcccggttg agagcggata gctcggagtc gtcgcggaag cggctgcacg
      241 agcggtccac ccggtcgagg accgtgtcga ccgcggcccg ggctgcggcc gcccggtcgg
      301 ggtcggccac caggaccacg gccccggtgc cgaacaccgg gaacgccacg cgcgccgcct
      361 gccccgcggc ggccccctct accgtgcgca tcacgaaccc cccgaggtga cccggccgcg
      421 cctcgtggtt gcctgcggga cgctcgccgg ctgctggagg ccgtcgtcgg cggagctaga
      481 gctggccagg ccggtgccgg tcgtggtggt agtggtcgcg ttggcggagg agctcgtgcc
      541 ggcgctggtg gtcgccgccg cggggtggcc ggcgaaggcc tgggccgcca ccccggcgag
      601 caggccggtc agcgccacgc tccccgcggc cacccagcgg gtggcgcggg ccagccggcg
      661 gaggccgaga tcgcggtcgg cgggcgaagg cacggggtca gtctgacggc cacggctgaa
      721 gatctgctgt gcacttgctc aaagtgccct ccgatatcgg ggcccggcag cgggcccggc
      781 agcctgctag aagggagcga tgccccgcct gacgagcatg gacgagcaat tcgtccatca
      841 ggtccccgag cccctcccgc gcgtggccac ctggcacgag cactggcgcg agagcctgtt
      901 cttcatcatg caccggccgg acgggccagg agacgtgctc atcctcacgc tcgcccattt
      961 ccccgcccgc caggagatgg atgccctcca gctgggacgg gtcggcggcc agccggtctt
     1021 cgcccgcgtc gcccgcccct acggcgacga cccccacacc atggccgtcg gcccggtgcg
     1081 catcgacatc gccgagccct accggaccgt gcacctgcac gtggacgagg accccgcggc
     1141 cgcggtggcg ctggagctga ccttcgccgc ccggacccgc gcccatggcc tgcggcgcgg
     1201 caccatgaaa tggcggcacg agacggtctg ggaccagagc cacatgatcc agtcgggcac
     1261 gttcaacggc accgtcagcc ggggcggcga gaccttcgag atcgacaact ggtggggcca
     1321 gcgcgaccac tcctggggca tccgggacca cgcccgctgc ccgttgtgga tctggctcgc
     1381 catccagttc cccgagggga tgctgggggt gtggcactgg gagtacccca acggcgcccg
     1441 ggtctacacc gacggctgct tcgccccggc cggcgacgcc gacccggtgc ccgtcgtcga
     1501 cttccagcac gacctgcggt ggctggacgc ggccggcgcc gaggtgtcct acggccggga
     1561 cggggagaag gtgaccggcc tggccggcca ggtggtgttc accttggagg gggggcggcg
     1621 gatcaccgtc gacgccgagg gccggtgggc gcagcgttac ggcccgctcg ggggaggcct
     1681 gtcggagatg gcggtgcgga cctccgacgg gtggcagggc acggcgatct acgaggtgac
     1741 cggcgccttt caccaccggt acttcccgga ggcccgggcc gagcggctgc cacccggcga
     1801 gtagccctcg gcgatcgact ggacccctgg gccgtcgccg tgacaacctg acgcccgatc
     1861 gtccgcccag gcccggacaa gggggcccga acaaggggga gagatgcctc atccgttcct
     1921 gtccagcacg tggttcgacg agatggacaa gctccgggcc gaggccccgg agcccaccgg
     1981 tgccaccgcc gacctgacgg tcaacatcgt cgtcaccggg gccccggagg gcgacgcgga
     2041 ggtccacatc aagggtgggc agttcgaccg aggcctgctg gagggtgcgc cgaccaagct
     2101 gaccgtgccg tatgccgtgg ccaagtcgat cttcatcgac ggcaaccagc aggccgggat
     2161 gcaggccttc atgtccgggc agatcaaggt cgagggcgac atgaccaagc tcatggccat
     2221 gcaggccggt ggcactccgt cagcggagca gcaggcgttc cagcagaagc tgaaggactt
     2281 gaccgagtag gtggcaccgg gccggccggc gcccggccgt cacgggccgg gcgtggtggt
     2341 ggtcgtcgtg gccgccggca gggagagcga tctgcacgcc ggctggccga gggcggaggt
     2401 gagcttgggg tcccggtagg tccggccgat ctggctgatg cgggccgtga tctgcttgag
     2461 cgcggagtcg agcatcgcgc tgatggcctg cacgtcctgg gtgtacttgg cgttgtcgtc
     2521 gaggggcagg ccggcggccc tggtcttggc gtcggagtac gtggtctgga acgccgccag
     2581 ggtcgacacg taggcgtcct ccgccgccgg gccctccttc accgcgggat ggcctgcgtc
     2641 gcgcaccgtg gtgaccatct gggccgacgt ctgccccagc gcgtcgaaga gcccgaccag
     2701 ctggtcccgc tggtcggccg ccagggtcag ggtcgcggtc ttggtccgga acgcggccag
     2761 acggctggcc gtgtcctgct gccaggcgtt ccacgcctcg cagacggtct ggccccactt
     2821 cttcggcgcc accccgctgt agtgggggtg gctcccgcag gcggtcacgg ccgccccggg
     2881 cccggccaca gctaccgcgg cggcgaccgc cgccgcccgg agggcgggcc gccgcggcct
     2941 gccggtcgcc gatcggacag ccatgggtgg caccgtagcg gccctgcccg tctgatggct
     3001 ctggtcgctt tggtctcggt ccggtcgtgc cgatacctcc tgggagcgga ggaggtctcg
     3061 cagtgcgcgt gcccggagac gagcggttga cagcccgtag cggtcggggg ggcccggcgc
     3121 cggtgacggt gtcggcatgg gaggcccatc tgggccggcg gtcctatgtg ccctggcagt
     3181 gcggcgactg cgaggccgtc ctgccgttcc cgaaggaggc cgaggtcgcc tgcccgcggt
     3241 gcggccaggg cgcggaagcc tcggcccgcc tggcgtcacg gctcgaatcg gccgccggtg
     3301 ccgcgtcgga ccccgccacc cgggcctggc tgctccacac cgcctcggcc tggcgcgggt
     3361 tgcccgggcg gacgccggcc ggctaggccg gaccggccgg accggcctga ggggtcgccc
     3421 tgggggcgtg cacctgccag gatcggcggt atgcgcgccc tcaccgtggt accgggccag
     3481 gcgggctccg cccggctcga cgacgtcgac gatccgcccg actccgacgg ccccgtgctg
     3541 gtggagaccc tggccatcgg ggtctgcggc accgacgccg agatcatcgg gggcgcctac
     3601 gggtgggccc caccgggccg gga
//
LOCUS       DASWGG010000293         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_448975, whole genome shotgun sequence.
ACCESSION   DASWGG010000293 DASWGG010000000
VERSION     DASWGG010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448975"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..620)
                     /locus_tag="VF954_05300"
     CDS             complement(186..620)
                     /locus_tag="VF954_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530532.1"
                     /translation="MTGRVRAAAAVVGLVACGGGHSPSGVPATTRPTTTTMATTAAPA
                     PTTSSVAAVVAGGSSPEDAAGHLLAAWKAGDRAAALTEADRTAVDSLFARPYPSAGFQ
                     ARGCDQNPNASNCFYRIGDNGLNLHTVHAPVGWRVDAAQLVQ"
     gene            783..>1636
                     /locus_tag="VF954_05305"
     CDS             783..>1636
                     /locus_tag="VF954_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9530533.1"
                     /translation="MPQETEAEFRARVQAFFSAHAPRRRVRPDPDAAEPVAPEAAEGQ
                     DEGGHGFLSADDGDMVSRSKAFQAAMFDAGLAGLTWPREYGGQGLTNREIQIWGEESA
                     AYELPASIYTIGHGMCGPTILQLGTEAQKQRYIRPMLRGDEVWCQLFSEPGAGSDVAS
                     LQTRAVRDGEEWVVNGQKVWSSGAHYCDWGVVITRTNLDNPKHRGISMFIVDMHSPGV
                     TVRPLRQITGGSNFNEVFFDDVRIPAENLIGEVDEGWRVALGTLMVERFAVNQLLDNA
                     VPAIEHAR"
BASE COUNT          226 a          577 c          617 g          216 t
ORIGIN      
        1 ggcccatacg cggctggctg gaggcgggcg ggctggccga cgccggcgac gtgctggcca
       61 ccatcctcgg gtggcgttcg acgccccggg ccgggcggtg gatggtgctg gccgcggcga
      121 gcggcggggt ggccgccgcc gccgccctgg cgccgcgggt ggactgagcc acagcacgag
      181 ctgggctact gcacgagctg ggcggcgtcc acccgccagc cgacaggggc gtgaacggta
      241 tggaggttga ggccgttgtc gccgatccgg tagaagcagt tggacgcgtt cgggttctgg
      301 tcgcacccgc gggcctgaaa gcccgcgctg gggtagggcc gggcgaacag agagtcgacg
      361 gcggtccggt cggcttcggt cagcgccgcc gcccggtcgc ctgccttcca ggccgccagc
      421 aggtgcccgg ccgcgtcctc cggcgacgac cccccggcca ccaccgccgc aacgctcgac
      481 gtggtgggcg ccggcgcagc cgtcgtggcc atggtcgtgg tggtcggccg cgtcgtcgcc
      541 ggcacgcccg agggcgaatg gccgccgccg cacgccacca gcccgacgac ggccgccgcc
      601 gcccgcaccc ggcccgtcac gggttctcag catagggcgg cagcatcggg gcagcagcat
      661 cggcctgggg cggcgcggcg gcccgctggg caccggcgcc atttccccct acgacagcgc
      721 cctttgggag gaccgcaccc ggtccaggta gggtcgtgag cagattccac ggaggagggg
      781 caatgccgca ggagaccgag gccgagttcc gagcgcgggt gcaggccttc ttttcggccc
      841 acgcgccccg ccggcgggtc cgtcccgatc ccgacgccgc cgagccggtc gctcctgagg
      901 cggccgaggg gcaggacgag ggcggccacg gctttctctc ggcggacgac ggggacatgg
      961 tctcccgcag caaggccttc caggcggcga tgttcgacgc cggcctggcc gggctgacgt
     1021 ggccgcggga gtacgggggt caggggctca ccaaccgcga gatccagatc tggggcgagg
     1081 agtccgccgc ctacgagctg cccgccagca tctacaccat cggtcacggc atgtgcggcc
     1141 ccaccatcct ccagctgggc accgaggccc agaagcagcg ctacatccgg cccatgctcc
     1201 ggggcgacga ggtgtggtgc cagctgttct ccgagccggg cgcgggctcg gacgtggcct
     1261 ccctccagac ccgggccgtg cgcgacggcg aggagtgggt cgtcaacggg cagaaggtct
     1321 ggtcctccgg cgcccactac tgcgattggg gtgtggtcat cacccgcacc aacctcgaca
     1381 accccaagca ccgggggatc tcgatgttca tcgtcgacat gcactcgccg ggagtgaccg
     1441 tgcggcccct gcgccagatc acgggcgggt ccaacttcaa cgaggtgttc ttcgacgacg
     1501 tgcggatacc ggccgagaac ctgatcggcg aggtcgacga gggctggcgg gtggcattgg
     1561 gcacgctgat ggtcgagcgc tttgcggtga accagctgct cgacaacgcg gtgccggcga
     1621 tcgagcacgc ccgcca
//
LOCUS       DASWGG010000294         2547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2839335, whole genome shotgun sequence.
ACCESSION   DASWGG010000294 DASWGG010000000
VERSION     DASWGG010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2839335"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VF954_05310"
     CDS             <1..835
                     /locus_tag="VF954_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9530534.1"
                     /translation="KKDSKVYEPSGRFGAVTSTDGRIGTQTMPGIFAQIGMEYGNRYG
                     GTSFELFARISEKNHAHSTLNPLAAYTKKMSLDEIMNDVMIAYPNTRPMCSANCDGAA
                     AAVVVNGETLKTLSGEQQRRAVKVSASVLTTDPWQEACQVLPDINTLTRNAATQAYEQ
                     AGVAPEDLDLVELHDCFATAELVHYDNLMLCPTGGAVELFESGATWRDGRIPVNVSGG
                     LESKGHPIAATGIANIWEICHHLRGEAGDRQIAGAKVGLAQVIGLGSACGVHILEKAA
                     A"
     gene            complement(891..>2547)
                     /locus_tag="VF954_05315"
     CDS             complement(891..>2547)
                     /locus_tag="VF954_05315"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9530535.1"
                     /translation="GAYEVVPARSDSIMITVTPNAPAVFAWMIEAGFEEVNSLLEVPL
                     EQLVRRIADVMKVTSSFAQLDDASVLWLAAQQRRLAWAEVCSVPKVAANPQHLFRQFF
                     RDVDWAGPRVQVPGPLARFGATPAPPPRPPDPEPVAIESLVREWAQPRPDAGRTGPDP
                     GAKPLDGLRVLDLTHVLAGPHATRLLGDLGADVVKLQTAERATSVNDPAFPYFYVWNR
                     SKRDISLNMAHPRAAEMARRLVEHSDVVIENFSAGVLDRWGLSYETVRSWNPRIVYVT
                     MSGCGHEGPWSHLLTYAPTIHALSGLTYLSNPRGRTDVGPGFSLNDHAAGLSAAVAVL
                     AALEARERTGEGQHVDISQMETGTYIVGAALLDLLSNGRDTEPDGNVDPFSDVAPNEC
                     YPTADHEWVAVTCRDDDEWRRLLAATGIDADPSLAAAPARRQRLAEVDAAVAAWALTV
                     TADEAQERLQAVGVPAGKVQHPGHLMEDPQLRARGLWRTRDHAVFGERPYDRFPAIWS
                     GTTLEPYLPSPTYVGEHNFEVYGALAGLDETEIAVGISDNLFA"
BASE COUNT          389 a          881 c          936 g          341 t
ORIGIN      
        1 caagaaggac tccaaggtgt acgagccctc cggacggttc ggcgccgtga cgtcgaccga
       61 cgggcgcatc ggcacccaga cgatgcccgg catcttcgcc cagatcggca tggagtacgg
      121 caaccgctac ggcgggacca gcttcgagct cttcgcccgg atcagcgaga agaaccacgc
      181 ccactcgacg ctcaacccgc tggccgcgta caccaagaag atgtcgctcg acgagatcat
      241 gaatgacgtc atgatcgcct atcccaacac ccgcccaatg tgctcggcca actgtgacgg
      301 cgccgcggcg gccgtggtcg tcaacggcga gacgctcaag acgctctcgg gcgagcagca
      361 gcggcgagcg gtgaaggtca gcgcgtcggt cctcacgacc gatccctggc aggaggcctg
      421 ccaggtcctg cccgacatca acaccttgac ccgcaacgcc gcaactcagg cctacgagca
      481 ggcaggagtc gccccggagg atctcgacct ggtggagctg cacgactgct tcgccaccgc
      541 cgagctggtg cactacgaca acctcatgct gtgcccgaca ggcggcgccg tcgagctgtt
      601 cgagtcgggc gccacctggc gcgacggccg catcccggtc aacgtgtcgg gcggcctgga
      661 gtccaagggc catcccattg ccgccaccgg catcgccaac atctgggaga tctgccacca
      721 cctgcggggc gaggccggcg accgccagat cgccggggcc aaggtgggcc tggcccaagt
      781 catcggccta gggtccgcct gcggcgtgca catcctggag aaggccgcgg cctagcgagc
      841 gccgccaccg gccccattcg gggccgctcg gcgatcatac gaacaggcga tcaggcgaac
      901 aggttgtccg agatcccgac ggcgatctcc gtctcgtcga ggccggcgag cgcgccgtac
      961 acctcgaagt tgtgctcgcc gacgtaggtc ggcgacggga ggtagggctc cagggtggtg
     1021 ccgctccaga tggccgggaa ccggtcgtag ggccgctcgc cgaacacggc gtggtcacgc
     1081 gtgcgccaca gcccccgggc ccgcagctgc gggtcctcca tgagatggcc ggggtgctgg
     1141 accttgcccg cgggcacccc gacggcctgc aaccgctcct gggcctcgtc cgcggtgacc
     1201 gtgagcgccc aggccgccac ggcggcatcc acctccgcca ggcgctggcg ccgcgccggg
     1261 gcggcggcga gcgacgggtc ggcgtcgatg ccggtcgcgg cgaggaggcg gcgccactcg
     1321 tcgtcgtccc ggcaggtgac ggccacccac tcgtggtcgg ccgtggggta gcactcgttg
     1381 ggcgccacgt cggagaaggg gtcgacgttg ccgtcgggct ccgtgtcgcg gccgttgctc
     1441 agcaggtcga gcagcgccgc gccgacgatg taggtgccgg tctccatctg cgagatgtcg
     1501 acgtgctggc cctcgccggt gcgctcccgg gcctcgagcg cggccaggac ggcgaccgcg
     1561 gccgacagcc cggcggcgtg gtcgttgagg gagaacccgg gcccgacgtc ggtccggccc
     1621 cgggggttgg agaggtacgt gagccccgac agggcgtgga tggtgggggc gtaggtgagc
     1681 aggtgcgacc atgggccctc gtggccgcag ccggacatcg tcacgtacac gatgcgaggg
     1741 ttccaggacc ggacggtctc gtacgacagg ccccatcggt cgagcacgcc ggcggagaag
     1801 ttctcgatga ccacgtcgct gtgctcgacc agccggcgcg ccatctccgc cgcccgcggg
     1861 tgggccatgt tgagcgagat gtctcgcttg gagcggttcc acacgtagaa atagggaaac
     1921 gccgggtcgt tcacgctcgt ggcccgctcc gccgtctgca gcttgaccac gtccgcgccg
     1981 aggtcgccca gcaggcgcgt ggcgtggggg ccggccagga cgtgggtgag atccaggacg
     2041 cgcaggccgt cgagcggctt ggcgccgggg tccgggccgg tccgccccgc gtcgggccgg
     2101 ggctgggccc actcccgcac cagcgactcg atggccacgg gctcggggtc cggcgggcgg
     2161 ggcggcggtg cgggcgtggc gccgaagcgg gccagggggc cggggacctg gacccggggg
     2221 ccggcccagt cgacgtcccg gaagaactgc cggaagagat gctgggggtt ggcggccacc
     2281 ttgggtaccg agcacacctc cgcccatgcc aggcgccgct gctgggcggc gagccagagc
     2341 acggaggcgt cgtcgagctg ggcgaacgac gaggtcacct tcatgacgtc ggcgatcctc
     2401 ctcacgagct gctcgagcgg cacctccaga agcgagttga cctcctcgaa gccggcctcg
     2461 atcatccagg cgaagacagc cggggcgttg ggcgtgaccg tgatcatgat cgagtcggac
     2521 cgggcgggca cgacctcgta ggcgccg
//
LOCUS       DASWGG010000295         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2839445, whole genome shotgun sequence.
ACCESSION   DASWGG010000295 DASWGG010000000
VERSION     DASWGG010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2839445"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VF954_05320"
     CDS             complement(<1..585)
                     /locus_tag="VF954_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX9530536.1"
                     /translation="MDLVDAPFLRACRNLPVARRPAWFMRQAGRSLPEYRALRGEGSM
                     LDTLTKPELAAELTVQPVRRYGVDAAILYSDIMVPVAAIGFGVDIVPGVGPVVDQPFR
                     GPSDLERLRPLEPGVDTPHVLETIRILREELTVPLIGFAGAPFTVASYLLEGRPSRDY
                     VRTKQLLHSDPALWDQLLDRLADLALASLRSQVEA"
     gene            761..2917
                     /locus_tag="VF954_05325"
     CDS             761..2917
                     /locus_tag="VF954_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEX9530537.1"
                     /translation="MRESLRERIASVIDQRSGTPQAMAERDIVVRTSLLRIEQLDLGR
                     ESLCFGRIDREASDGDLGESFYIGRLAVSGDDMEPLVVDWRAPVAEPFYRATGRHTMG
                     LRRRRHFATDGARLLGIEDEVFSVDGAGGLPDTDGLEVLDGHPGGRGGGDGDGNGADR
                     AAFTATGALLAALERSRSGHMRDIVATVQREQDEIIRAPLPGVLVVQGGPGTGKTAVA
                     LHRAAYLLYTHRFPLERQGVLVVGPNPVFLRYISQVLPSLGESGVALTTVEGLVPQAR
                     VRADDAPDVAFLKGDERMAMVLARAVRDRQRPLRVPLEVPYGSYLLRLTTDDSASVVA
                     AARRRPGTHNARRRFVEAQVAQRLYRRLQDAADRALRTGVRPIGGDSGEVDGESFARD
                     VRRRPEVMAALDRMWPRLLPEELLHDLYGAPALLHLAAGHALSGEEVRRLHRPRRDAL
                     DSIPWTPGDLPLLDEAAVLLGPVRAGSPGVDGVRTYGHIVVDEAQDLSPMQLRMLTRR
                     SISGSMTVVGDIAQATGARSPSGWDEITPHLPARKPVMVAELSVNYRTPAEIMELAGR
                     VLREAAPDLRPPRSARVTERPPAVVAAAPADLTERVVAEAAELSAWLRNGMVAVIAPD
                     SLMAELRSRFGASGLPVGEADRDGLDRPITLVPVAEAKGLEFDGVIVVEPGRILAEMP
                     QGLRSLYVALTRATQRLVVVHAEALPAPLQGTSGRG"
BASE COUNT          396 a         1098 c         1134 g          380 t
ORIGIN      
        1 ggcctcgacc tgcgaacgca gcgacgcgag cgccaggtcg gccaggcggt ccaacagctg
       61 gtcccacagc gccgggtccg agtggaggag ctgcttggtg cggacgtagt cccgggacgg
      121 gcgaccttcc aacaggtagc tggccacggt gaacggcgcc ccggcgaacc cgatcagcgg
      181 gaccgtgagc tcctcgcgga ggatccggat ggtctccagg acgtggggcg tgtcgacccc
      241 gggctccagc gggcggagcc tctcgaggtc cgacggcccg cgaaagggct ggtcgacgac
      301 cgggccgacg ccggggacga tgtccacccc gaagccgatg gcggcgacgg gcaccatgat
      361 gtccgagtac aggatggcgg cgtcgacccc gtagcggcgc acaggttgca ccgtgagctc
      421 ggcggccagc tccggcttgg tcagcgtgtc cagcatgctg ccttcgcccc ggagcgcccg
      481 gtactcgggg agcgaccgtc ccgcttggcg catgaaccag gccggacggc gcgccaccgg
      541 caggttgcgg cacgcccgca ggaagggtgc gtcgaccagg tccacccgcc cgacagtacc
      601 tggggccccg tgccgagccg gaagcgggca gtacactttt gcgttccccg ttccggaacg
      661 acgctcgggc ccggggcccc agaaagacgc cgtggcgaca cacccagacc tccaggcgga
      721 gcaggcgtac atcgacgagg cctaactgcg cctgggcgcc atgcgggagt cgttgcggga
      781 gcggatcgcc tccgtcatcg accagcgcag cggcacgccc caggcgatgg ccgagcgcga
      841 catcgtcgtg cgcaccagcc tgctgcgcat cgagcagctg gacctgggcc gggagtcgct
      901 gtgcttcggg cgcatcgaca gggaagcctc ggacggcgac ctgggggaga gcttctacat
      961 cggacggctc gccgtatccg gagacgacat ggagccgctc gtggtggact ggcgggcgcc
     1021 ggtggccgag cccttctacc gggcaaccgg ccggcacacg atggggctgc gccggcggcg
     1081 gcattttgcg accgacggcg cccgcctcct gggcatcgag gacgaggtct tctcggtcga
     1141 cggcgccggc gggctcccgg ataccgacgg cctcgaggtc ctcgacggcc acccgggcgg
     1201 gcggggcggc ggggacgggg acggcaacgg cgcggacaga gcggcgttca ccgcgacggg
     1261 cgccctgctg gccgcgctgg agcggtcgag gtcgggccac atgcgggaca tcgtggcgac
     1321 cgtgcagcgc gagcaggacg agatcatcag ggcacccctg cccggcgtcc tggtggtcca
     1381 gggcggcccg ggcacgggca agacggccgt tgccctccac cgggccgcgt acctgctcta
     1441 cacccaccgc ttcccgctcg agcgccaggg ggtgctggtc gtcgggccca acccggtgtt
     1501 cctgcgctac atatcccagg tgctgccctc gttgggcgag tcgggcgtcg cgctcaccac
     1561 cgtggagggc ctggtgcccc aggcccgggt gcgggccgac gacgcgcctg acgtggcgtt
     1621 cctcaaaggc gacgagcgca tggccatggt cctggcccgg gcggtgcgtg accgccagcg
     1681 gcccctccgc gtcccgctcg aggtcccgta cggctcctac ctgctccgcc tgaccacgga
     1741 cgactcggcg tccgtcgtcg ccgcggcccg gcgcaggccg ggcacccaca acgcccgccg
     1801 acggttcgtg gaggcccagg tggcccagcg gctgtaccgc cgcctccagg atgcggccga
     1861 ccgggccctg cggaccggcg tccgccccat cggcggggac agcggcgagg tcgacgggga
     1921 gagcttcgcc cgggacgttc ggcgccggcc cgaggtcatg gccgcgctgg accgcatgtg
     1981 gccccgcctc ctgcccgagg agctgctcca cgacctctac ggggcgccgg cgctgctcca
     2041 tctcgccgca gggcacgcgt tgagcggcga ggaggtccgc cgcctccacc ggccgcgccg
     2101 cgacgccctg gactcgatcc cgtggacgcc cggggacctc ccgctcctcg acgaggccgc
     2161 cgtgctgctc ggccccgtgc gggccggctc gcccggtgtc gacggggtcc ggacctacgg
     2221 ccacatcgtg gtcgacgagg cccaggacct gtcacccatg cagctgcgga tgctgacccg
     2281 ccgctccatc tccggctcga tgaccgtcgt cggcgacatc gcccaggcca ccggtgcccg
     2341 ctcgccctcc ggttgggacg agatcacgcc gcacctgccg gcccgcaagc cggtcatggt
     2401 ggcggagctc agcgtcaact accgcacgcc cgccgagatc atggagctcg ccggccgcgt
     2461 cctgcgggag gcggcgccgg acctgcggcc accgcggtct gcgagggtga ccgagcgacc
     2521 gcccgccgtg gtcgcggcgg cacccgccga cctgacggag cgggtggtgg ccgaggccgc
     2581 ggagctgtcg gcctggttgc gcaacgggat ggtggcggtc atcgcacccg actcgctcat
     2641 ggcggagctc cgctccaggt tcggcgcctc cgggctgccg gtcggagagg ccgatcgcga
     2701 cggcctcgac cggcccatca ccctcgtgcc cgtggccgag gccaagggtc tcgagttcga
     2761 cggcgtcatc gtggtggagc caggccgcat cctggccgag atgccccagg gtctgcgctc
     2821 gctgtacgtg gcgctcaccc gcgccaccca acggttggtg gtggtccacg ccgaggccct
     2881 gccggcgccc ctccagggca cgagcggccg ggggtgaggg ggggcgcggt gggtgagggg
     2941 ggccgggcgg ccgggcgggc ctcccggcgg ggcggagctc aactggggac agaaacccac
     3001 cgtggtgg
//
LOCUS       DASWGG010000296         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_449598, whole genome shotgun sequence.
ACCESSION   DASWGG010000296 DASWGG010000000
VERSION     DASWGG010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449598"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..>1778)
                     /locus_tag="VF954_05330"
     CDS             complement(167..>1778)
                     /locus_tag="VF954_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX9530538.1"
                     /translation="RAVTWGEPGWESEDPAGAQAAYDQLAGLTVKKAQDQIVAQLRQS
                     GDLVGEPRPVTHAVKFWENGRRALEIITNRQWFIRTMAHRDELLALGRQLRWYPDWMQ
                     VRYENWVNGLNGDWNITRQRFFGVPFPLWYPLGPDGVPDYERPIVPDRARLPVDPSSD
                     VPEGYTEDQRDRPAGFTGDPNVMDTWATSSVTPQIVGGWAEDPDLFARVFPMDLRPQA
                     HEIIRTWLFYSVVRSHFEHGVLPWREAAISGFVIDPDRKKLSKSKGNSPDDPVALIAD
                     YGSDAVRYWSANGRMGVDTAFDRNQFKIGRRLATKVLNVSCFVLPRLGTDGPDLGVTE
                     PLDAAALVRLARVVEEATGAFDTYDYTRALERTESFFWELCDDYVELVKGRAYGTAGE
                     AGAASASRALGAALSTLLRLFAPFLPFVTEEVWSWWQEGSIHRARWPDAAELRSLGGN
                     GDPRVLEVTAAVLAQVRKAKSQAKLSMRAPVAHLSVRCDASQAALLGSALADLADAAG
                     VVDGVGGVEVRVVTPEVGLPVAEVTLVE"
BASE COUNT          254 a          652 c          629 g          243 t
ORIGIN      
        1 ctctgcaggc gagaggaccg cgacgccagc tcgagcggca ccaccaccgc gcagccccat
       61 ggcgggggcc ggcgcaggtc gagctcgggc gccgggcccg gccggcggcg tcgccatctc
      121 atgctggcac gatcgaccct ctggctggga agggcctgag agctacctac tccacgagtg
      181 tgacctcggc gacgggcagg ccgacctccg gcgtcacgac gcgcacctcg acgcccccca
      241 ctccatcgac cacgccggcc gcgtccgcca ggtcggcgag cgcggagccc agcaaggcgg
      301 cctgggaggc gtcgcagcgg accgacaggt gcgcgaccgg cgcgcgcatc gacagcttgg
      361 cctggctctt ggccttgcgc acctgggcca ggacggcggc ggtgacctcg aggacgcgag
      421 ggtcgccatt tcccccgagg cttcgcagct cggccgcatc cggccaccgc gcccggtgga
      481 tcgacccctc ctgccaccac gaccacacct cctcggtcac gaacggcagg aacggggcga
      541 acagccgcaa cagcgtcgac agcgccgcgc cgagcgcccg gctggccgag gccgcgcccg
      601 cctcgccggc cgtcccgtag gcccggccct tgaccagctc cacgtagtcg tcgcagagct
      661 cccagaagaa gctctccgtc cgctccagcg cccgcgtgta gtcgtatgtg tcgaaagctc
      721 cggtcgcctc ctccacgacc cgcgccaggc ggaccagcgc ggcggcgtcc aggggctcgg
      781 tcacgccgag gtcgggaccg tcggtgccca gccggggcag cacgaagcag ctgacgttga
      841 gcaccttggt ggccagccgg cgcccgatct tgaactggtt gcggtcgaag gccgtgtcca
      901 cgcccatgcg cccgttggcc gaccagtagc ggaccgcgtc cgagccgtag tcggcgatga
      961 gggcgaccgg gtcgtcgggg ctgttgccct tggacttgga cagcttcttg cggtcgggat
     1021 cgatgacgaa gccggagatg gccgcttccc gccacggcag gaccccatgc tcgaagtgcg
     1081 aacgcaccac gctgtagaac agccacgtcc tgatgatctc gtgggcctgg ggccgcaggt
     1141 ccatggggaa tacccgggcg aagaggtcgg gatcctccgc ccacccgccc acgatctggg
     1201 gggtgaccga cgaggtggcc cacgtgtcca tcacgttggg gtccccggtg aagccggcgg
     1261 gccggtccct ctggtcctcg gtgtagccct cggggacgtc ggaggacggg tcgaccggca
     1321 gccgcgcgcg atcggggacg atgggccgct cgtagtccgg caccccgtcg gggcccagcg
     1381 ggtaccacag ggggaagggc acgccgaaga accgctggcg ggtgatgttc cagtcgccgt
     1441 tgaggccgtt gacccagttc tcgtagcgga cctgcatcca gtccgggtac cagcgcagct
     1501 gccggccgag cgccagcagc tcatcgcggt gggccatggt gcggatgaac cactgccggt
     1561 tggtgatgat ctccagggcc cggcggccgt tctcccagaa cttcaccgca tgggtgaccg
     1621 gccggggctc gcccaccagg tcgcccgact ggcgcagctg ggcgacgatc tggtcctgcg
     1681 ccttcttcac cgtgagcccg gccagctggt cgtaggctgc ctgcgcgccg gccgggtcct
     1741 cgctctccca gccgggctcg ccccaggtga cggcccgc
//
LOCUS       DASWGG010000297         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1244272, whole genome shotgun sequence.
ACCESSION   DASWGG010000297 DASWGG010000000
VERSION     DASWGG010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244272"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VF954_05335"
     CDS             <1..560
                     /locus_tag="VF954_05335"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530539.1"
                     /translation="SLSAGAALVTFSGDKLLGGPQAGIIAGRAELVDRCTRHPLARAL
                     RPGRLVLGAMQDVALAYLRRDGAAIPFWRMAALSEESLRARAAALGVGEVVACESVPG
                     GGAVPGRTIPSAGVAVDGDVTAALRAWDPPIVARVSEGRTVLDLRTVDEADDDVVAAA
                     LSHEPGRGPNPGLLPDVGPQPHAGP"
     gene            577..>1831
                     /locus_tag="VF954_05340"
     CDS             577..>1831
                     /locus_tag="VF954_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9530540.1"
                     /translation="MHVVATAGHVDHGKSTLVLALTGTDPDRFAEEKARGLTIDLGFA
                     WTVLPSGRELAFVDVPGHVRFIKNMLAGVGAVDACLFVVAATEGWKPQSEEHLRILEL
                     LGVQHGVVALTKAGLVDEDWLELARLDLADHVAGTFLAGAEVAAVDAPSARGLEELTG
                     ALDRLLATAPTAVDRDRPRLWVDRVFTARGSGTVVTGTLAGGPLRLDDELLVVPTSGG
                     RPLPARVRGLQTHQMPRGDVGPGHRVAVNLAGVARDRLVRGDALVRPGQWEPTRTFDA
                     SLTVLAGLGHEVSRRGAFQAYVGSGEQPVRVRVLGSDAIEAGHTGAVRLHLPVALPLL
                     PGDRYVLRESGRAETVGGGQVLDVAPVLPAARARPDRSVERVVAERQWVDAGLLARLT
                     GTHPAPTVGRWVASPEALAGARARLR"
BASE COUNT          195 a          704 c          703 g          229 t
ORIGIN      
        1 agtccctctc cgctggcgcg gccctggtca ccttctccgg ggacaagctg ctcggcgggc
       61 cccaggcggg gatcatcgcg ggccgggccg agctggttga ccgctgcacc cgccacccgc
      121 tggcccgggc cctccgcccc ggccgcctgg tcctgggggc gatgcaggac gtggccctgg
      181 cctacctgcg ccgggacggg gcggccatac cgttctggcg gatggcggcg ctgagcgagg
      241 agagcctgcg cgcccgggcc gccgcccttg gggtcggcga ggtggtggcg tgcgagtcgg
      301 tgcccggcgg gggcgccgtg ccggggcgca cgatcccttc ggccggcgtc gccgtggacg
      361 gcgacgtcac cgccgccctg cgggcctggg accccccgat cgtcgcccgg gtcagcgagg
      421 ggcgcaccgt gctcgacctg cgcaccgtag acgaggccga cgacgacgtc gtcgccgccg
      481 ctctgtcaca tgagcccggc cggggcccca accccggcct cctcccggac gtggggcccc
      541 aaccccacgc cgggccgtga cgcgccacag gccgtagtgc acgtcgtcgc caccgccggc
      601 catgtcgacc acgggaagtc gaccctggtg ctggccctga ccgggacgga cccagaccgc
      661 ttcgcggagg agaaggcccg gggcctgacc atcgacctcg ggttcgcgtg gacggtgctg
      721 ccgtcggggc gggagctggc attcgtggac gttccgggcc acgttcggtt catcaagaac
      781 atgctggccg gcgtgggcgc agtcgacgcc tgcctcttcg tggtggccgc caccgagggc
      841 tggaagccgc agtccgagga gcacctgcgg atcctcgagc tgctcggggt gcagcacggc
      901 gtcgtcgccc tgaccaaggc cggcctggtg gacgaggact ggctggagct ggccaggctc
      961 gatctggcgg accacgtggc cgggacgttc ttggccggcg ccgaggtggc cgcggtcgac
     1021 gcgcccagcg cccgggggct ggaggagctg accggcgccc tcgaccgcct gctggccacc
     1081 gcccccacgg ccgtcgaccg cgaccggccc cggctgtggg tcgaccgggt cttcacggcc
     1141 cgcgggtccg gcacggtcgt gaccgggacc ctggccggcg gcccccttcg cctggacgac
     1201 gagctgctcg tggtcccgac gtcgggcggg cggcccctcc cggcgcgggt gcgcggcctc
     1261 cagacccacc agatgccccg gggcgacgtg ggacccggcc accgggtcgc cgtcaacctg
     1321 gccggggtgg cccgcgatcg gctggtccgg ggcgacgccc tggtccgacc gggccagtgg
     1381 gagccgaccc gtaccttcga cgcttcgctc actgtgctcg ccggcctcgg ccacgaggtg
     1441 agcaggcggg gagccttcca ggcctacgtc ggctccggcg agcaacccgt aagggtccgg
     1501 gtgctcggga gcgacgccat cgaggccggc cacaccggcg ccgtacgcct ccacctgccc
     1561 gtcgccctgc cgttgctgcc aggcgaccgg tacgtcctgc gggagagcgg ccgggccgag
     1621 accgtcggcg gcggccaggt gctcgacgtg gcccccgtgc tgcccgccgc ccgggcccgg
     1681 cccgaccggt ccgtcgagcg ggtggtcgcc gagcgccagt gggtcgacgc cggcctgctg
     1741 gcccgactca ccgggacgca cccggccccg acggtgggcc ggtgggtcgc cagccccgag
     1801 gccctcgccg gcgcccgagc gcgcctgcgt g
//
LOCUS       DASWGG010000298         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1886550, whole genome shotgun sequence.
ACCESSION   DASWGG010000298 DASWGG010000000
VERSION     DASWGG010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1886550"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VF954_05345"
     CDS             complement(<1..595)
                     /locus_tag="VF954_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9530541.1"
                     /translation="MARILVTEEIAERGLAALRDAGYDVTVQLGLSPEELLEAVPGAA
                     ALIIRSATKVTADVLEAGGDLVVVGRAGIGLDNIDVPSATRRGVMVVNAPQSNILSAA
                     EHTIALLLAQARNLPQADSALRAGKWERSRWEGVELYGKTLGIVGLGRVGALVAQRAL
                     AFGMRLVAADPYVAPDRARQMGVELLSLEDVFGEADFL"
     gene            complement(643..>1581)
                     /locus_tag="VF954_05350"
     CDS             complement(643..>1581)
                     /locus_tag="VF954_05350"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9530542.1"
                     /translation="YPVAAAQVLVTNGAKQAIEEAFATLLDPGDEVLLPAPYWTTYPE
                     TVRLAGGVPVVIPTAAATGFRVTVDQLSAVLTPRTKVLLFVSPSNPTGAVYPRDEVEA
                     IGRWALEHGIWVVTDEIYEHLVYGSAVHYSMPVLVPELESQCIVVNGVAKTFAMTGWR
                     VGWMIGPLDVIKAATNLQSHATSNVCNVAQRAALAALTGDLDAALAMRAAFDRRRQTM
                     VAMLNEIPGVSCVEPEGAFYAFPSFEAVLGHTVAGRATRTTLELASTILDEARVAIVP
                     GEAFGFPGYARLSYALGDDELVEGLSRMGKLLAGAV"
BASE COUNT          266 a          549 c          536 g          230 t
ORIGIN      
        1 taagaaaatc ggcctccccg aatacatctt ccagcgagag cagctcgact cccatctggc
       61 gggctcggtc cggcgccacg taggggtcgg ccgcgaccag ccgcatcccg aacgccaacg
      121 cccgctgggc gacgagggcg ccgacgcggc ccagaccgac tatgccgagc gtcttgccgt
      181 acagctcaac gccctcccac cgggaccgct cccacttgcc ggcccttagg gcggagtcgg
      241 cctggggcag gttgcgggcc tgagccagga gcagggcgat ggtgtgttcg gcggccgaga
      301 ggatgttgga ctggggcgcg ttgacgacca tgacgccgcg acgggtagca gacggaacgt
      361 cgatgttgtc caacccgatc ccggcccggc cgaccaccac gaggtcgccg ccggcctcca
      421 ggacgtctgc tgtgactttg gtcgcagagc ggataatcag ggcggcggcg cccgggacgg
      481 cctcgagcaa ctcttcgggg gacaggccca actgcaccgt cacgtcgtag ccggcgtctc
      541 gaagggccgc cagccctcgc tcggcgatct cttcggtcac cagaatgcgg gccatgtcgc
      601 catcctcgcg gaccgcggga gtaatgagcg agccagcagt ggttagaccg ccccggcgag
      661 caattttccc atccttgaca acccctcgac gagttcgtcg tcgcccaggg cgtacgacaa
      721 ccgggcgtag ccaggaaacc cgaacgcctc tccgggcacg atggccaccc gcgcctcgtc
      781 caggatcgtc gacgccagct cgagcgtcgt ccgggtggcc cggcccgcca cggtgtggcc
      841 gagcacggcc tcaaaactcg ggaaggcgta gaaggctccc tcgggttcga cgcagctcac
      901 gccggggatc tcgttgagca tggcaaccat ggtctggcgg cggcggtcga aggccgcccg
      961 catcgccagc gcggcgtcaa ggtcgccagt cagggcggca agggccgctc gctgggcgac
     1021 gttgcacaca ttggaggtgg catgcgattg caagttcgtc gccgccttga tcacgtcgag
     1081 cggaccgatc atccacccca ctcgccagcc cgtcatggcg aaggtcttgg cgaccccgtt
     1141 gacgacgatg cactgcgact cgagctccgg caccagcacg ggcatcgagt aatggacggc
     1201 cgaaccgtag accaggtgtt cgtagatctc gtcggtcacc acccagatgc cgtgttcgag
     1261 ggcccaacgg ccgatggctt cgacctcgtc gcgcgggtac accgcgccgg tcgggttcga
     1321 cggcgacaca aacaacagca ccttggttcg gggcgtcagc acggccgaca gctggtccac
     1381 cgtgacccgg aacccggtcg ccgcggcggt gggaatcacg accgggacgc cgcccgccag
     1441 ccggacggtc tcggggtacg tcgtccagta cggcgccggg aggagcacct cgtcgccggg
     1501 gtcgagcaac gtcgcgaagg cctcttcgat ggcctgcttg gcgccgttgg tgaccagcac
     1561 ctgggccgcc gcgaccgggt a
//
LOCUS       DASWGG010000299         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1562479, whole genome shotgun sequence.
ACCESSION   DASWGG010000299 DASWGG010000000
VERSION     DASWGG010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562479"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VF954_05355"
     CDS             <1..459
                     /locus_tag="VF954_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9530543.1"
                     /translation="YTHSPSRELLADLAGGDVPRSTAADYEYFLGSAPHRAEMFYTGD
                     ADPAVVAADTAAAVESPSGEIQSVWSQPSGKVLGRITVPVYLQLGQQDRLFPPEYLPA
                     EGASFRSSPHVVTDVVPGAGHTFMLHPIGAESTFHLVSWVRNLPLTPPCS"
     gene            519..1493
                     /locus_tag="VF954_05360"
     CDS             519..1493
                     /locus_tag="VF954_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9530544.1"
                     /translation="MRRLLHVVACMVVTVSGFALAGRAPAATRGVGLLKQRVEFTVKN
                     PAENGATHQIVGYRYDTFCTSPTAVVLMHGLSYTKEAWDVPGYSVAQKLAEAGYAVIA
                     IDRLGYGESKLPNGYDVTHEAYADMAHQIVDQLRSQGFSHVVLGGHSAGAGTTELEAG
                     LYGGVDAIMALGWHHRPSNQLGQDFFTGDYLRAAQDNYEYFLGTPAHRAEMFYTPTAD
                     PDVVEADTKAAVPTPSGEIFSIGKQPSRLVVGNIKVPVFLQFGENDRLFEVQYAQMHA
                     EEFRSSPSVTVDIVKDAGHTFMLTKNGRIGADKMVEWLKSRPETPSCR"
     gene            1677..>2916
                     /locus_tag="VF954_05365"
     CDS             1677..>2916
                     /locus_tag="VF954_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9530545.1"
                     /translation="MQMTGAQALIKSLEMEGTEVMFGLPGGAILPVYDPLLESTIRHI
                     LVRHEQGAGHMAEGYAHATGRPGVAMVTSGPAATNIVTPLCDAMLDSIPFVCITGQVP
                     LAAIGTDAFQECDTTGITMPVTKHNWLIQRAEDIPTVVREAFYVATTGRPGPVLVDIP
                     KDISNQTMEWYWPDGVDLPGFRAPKPPDPQLAREAARLILAAERPVIYAGGGILKARA
                     AEALRRLAELTGIHVVTTLMARGAFPDDHPQCLGMPGMHGNYTAVTAMQQSDLLIALG
                     SRFDDRVTGKVPTFAPLAKIIHVDIDPAEIGKVRRADVGIAADCRAAIEAITAAVEAE
                     LAETATVAPDRADWNATLAGWQRAHPLSYDQPEDGPLKPQFCIEQLRDHTPDDTIVVS
                     GVGQHQMWSSQYWKFNHPYTW"
BASE COUNT          454 a         1121 c          928 g          413 t
ORIGIN      
        1 tacacccact cgccgtcccg ggagctgctg gccgacctgg ccggcggcga cgtcccccgg
       61 tcgaccgccg ccgactacga gtacttcctc ggctcggccc cgcaccgggc cgagatgttc
      121 tacaccggcg acgccgaccc ggccgtggtg gccgccgaca ccgccgccgc cgtggagtcg
      181 ccgtcgggcg agatccagtc ggtctggagc cagccgtcgg gcaaggtgct gggccggatc
      241 accgtccccg tctacctcca gctcggccag caggaccggc tcttcccccc ggagtacctg
      301 ccggccgaag gggcttcgtt ccgctcgtcg ccccacgtgg tcaccgacgt cgtccccggc
      361 gccggccaca ccttcatgct ccatcccatc ggcgccgaga gcacgttcca cctcgtcagc
      421 tgggtccgga acctccccct caccccgccg tgtagctgat cctggcgcga tctaccatgg
      481 agtaacactg tcgtctggtt ccaaggggga gggaagccat gcgccggttg ctccacgtcg
      541 tcgcttgcat ggtggtcacc gtcagcgggt tcgccctggc ggggcgggcg ccggccgcga
      601 ccaggggagt cggcctgctc aagcagcggg tcgagttcac ggtcaagaac ccggctgaga
      661 acggcgccac ccaccagatc gtgggctacc gctacgacac gttctgcacc tcgccgacgg
      721 ccgtcgtgct catgcacggc ctgtcctaca ccaaggaggc gtgggacgtc cccggctact
      781 cggtggccca gaagctggcc gaggcgggct acgccgtcat cgccatcgac cggctcggct
      841 acggcgagtc caagctgccc aatggctacg acgtgaccca cgaggcctac gccgacatgg
      901 cccaccagat cgtggaccag ctgcggagcc agggcttctc ccacgtcgtc ctcggcgggc
      961 actcggcggg ggcgggaacg accgagctcg aggccggcct gtacggcggg gtcgacgcca
     1021 tcatggccct gggctggcac caccggccct cgaaccagct cggccaggac ttcttcaccg
     1081 gggactacct ccgggcggcc caggacaact acgagtattt cctcggcaca ccggcgcacc
     1141 gggcggagat gttctacacg ccgaccgccg acccggacgt cgtggaggcc gacaccaagg
     1201 ccgccgtccc caccccgagc ggcgagatct tctccatcgg caagcagccg tcccgcctcg
     1261 tggtcgggaa catcaaggtg ccggtcttcc tccagttcgg cgagaacgac aggctgttcg
     1321 aggtgcagta cgcccagatg cacgccgagg aattccgctc ctcgccgtcg gtcaccgtcg
     1381 acatcgtgaa ggacgccggc cacacgttca tgctgaccaa gaacgggcgg atcggcgccg
     1441 acaagatggt cgagtggctc aaatcgcgcc cggagacccc gtcctgccgg taacggttgc
     1501 gggattcccc ggctctcggc gtagactgag ggccgtgttg gtcaccgccc tcattctcgt
     1561 agttccgtag cgcaccgagg cgtcccgcct cgctgcgctc tccgaccctc cgctctgcgg
     1621 ggggtctttc atttgctccc ccggagcccc gccttcccga cgaagggatc aggaccatgc
     1681 agatgaccgg cgcccaggcg ctcatcaaga gcctggagat ggaagggacg gaggtcatgt
     1741 tcggcctccc cggcggggcc atcctgcccg tctacgaccc cctcctcgag tcgaccatcc
     1801 gccacatcct ggtccgccac gagcagggcg ccggccacat ggccgagggc tacgcccacg
     1861 ccaccggccg gcccggcgtg gccatggtga ccagcggccc ggccgccacc aacatcgtca
     1921 cgccgctgtg cgacgccatg ctcgactcca tccccttcgt ctgcatcacg gggcaggtgc
     1981 cgctggcggc catcgggacc gacgccttcc aggagtgcga cacgaccggc atcaccatgc
     2041 cggtgacgaa gcacaactgg ctgatccagc gggccgagga catcccgacg gtggtgcggg
     2101 aggccttcta cgtggccacc accgggcggc ccgggcccgt cctcgtcgac atccccaagg
     2161 acatcagcaa ccagacgatg gagtggtact ggcccgacgg cgtcgacctg cccggattcc
     2221 gggcgccgaa gccacccgac ccccagctgg cccgggaggc ggcccggctc atcctggccg
     2281 ccgagcggcc ggtcatctac gccggcggcg gcatcctcaa ggcccgggcg gccgaggccc
     2341 tgcgccgact ggccgagctc accggcatcc acgtggtcac cacgctcatg gcccggggcg
     2401 ccttccccga cgaccatccg cagtgcctcg gcatgcccgg catgcacggc aattacaccg
     2461 cggtgacggc catgcagcag tcggacctcc tcatcgccct cgggtcccgc ttcgacgacc
     2521 gggtgaccgg caaggtcccc accttcgccc ccctggccaa gatcatccac gtcgacatcg
     2581 acccggccga gatcggcaag gtccgccggg ccgacgtcgg catcgccgcc gactgccggg
     2641 cggccatcga ggccatcacc gccgccgtcg aggcggagct ggccgagacg gccaccgtcg
     2701 cccccgaccg ggccgactgg aacgccaccc tggccggctg gcagcgggcc cacccgctgt
     2761 cctacgacca gccggaggac gggccgctca agccgcagtt ctgcatcgag cagctccgcg
     2821 accacacccc cgacgacacg atcgtcgtgt ccggcgtggg ccagcaccag atgtggagct
     2881 cgcagtactg gaagttcaac cacccctaca cctggg
//
LOCUS       DASWGG010000300         4607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3172887, whole genome shotgun sequence.
ACCESSION   DASWGG010000300 DASWGG010000000
VERSION     DASWGG010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3172887"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VF954_05370"
     CDS             <1..947
                     /locus_tag="VF954_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530546.1"
                     /translation="GAARPAPGSAGPGAPAQPGAMQPEAMQPAAPKARPSAAPAKPTK
                     QLREGDLICGQCGEGNDATRKFCSRCGNSLATAAKAHISWWRKIIPHRAPKKALKAGE
                     TKDKQKKDKLSSTIATGRKIFTYGGIAILLGAVAISLLQKADPSFKNPITDKVASARH
                     WVGHEYTTLKNFLLPSYEPVHSSGDIPTSALPDHPGVLAIDGFKNTYWAADGSRDKTP
                     TLGVHFDKKVTLKKMLITSGDSDNFQDEPRPQHIHIIYSNNKTQDVVLVDKADPQTVT
                     LSGAKDITDVHIQIVDTYTSLKGHDVSIAEVEFFRLKL"
     gene            complement(963..1727)
                     /locus_tag="VF954_05375"
     CDS             complement(963..1727)
                     /locus_tag="VF954_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530547.1"
                     /translation="MSPGDGERDGEGTFQLEDISFDGPNLDVPNPPAGTPYEEFEDYR
                     TARLLEANDLPTTADGIVHALQDQTGVLLAAAAHAAGSQSIGAAIPRLRQVVEGSDDQ
                     AAVEAAYALGRLGDAAARQTLVEAVSRPADAYLSALVAAGYLAQLGDPIGFPKVEEGL
                     GSGLSAIRMLACKQLYFFMPFDGANLPSGGPIDAAGAYQRALQDPDPDIQWQALAELR
                     LLRSPATKSLLDAYVERAQDEGLRRVARETLESLGS"
     gene            complement(1724..2866)
                     /locus_tag="VF954_05380"
     CDS             complement(1724..2866)
                     /locus_tag="VF954_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530548.1"
                     /translation="MVSPHISAVGADVEEDPRMTDWHRSGDSSDPLRSSRRTRPPGSA
                     PDAARQASALLARLAPGDRDQHLAQADMARLQHIAGNAAVSSLLERAPVTVDADGATT
                     ATVGAATTAAEEHVLPAPTLSFTATIKRADRDAVPGSEDTSELSGDDVVGTAGGEASD
                     IQEGQTASLPDIETPEDLALDTDAVDGSLTYAPSVAQSGTVSPFGSTTWSKFNITGIS
                     VTASSGTFKVSATVQNPITFNVDGGGDTDIASDSDSALTAANYATAASDLTPNMGDLG
                     GRPPRTQFWAKDLTVRHENFHATERQTFGGQGVTAAQTWLASQTASSAADVQSLLTQV
                     PGKVIAVSQAAMPYPAKEERAYGDGADAYKARADAITTKGAAGGYT"
     gene            complement(2937..>4607)
                     /locus_tag="VF954_05385"
     CDS             complement(2937..>4607)
                     /locus_tag="VF954_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9530549.1"
                     /translation="PDLDPRLERAYGYLHDDVSRRRASVGLAIELCGGDLVHPASRAR
                     LSPDAPLVAGGLLMIEDGERPFLTRSLRVPDIVAGHLLGDDVADPVVAPIVTTAVPVP
                     GYDTAPITRALEGGVRLVYLRDHPGTAALSYAAQSLRAAGRPLLALDLRRLTTGEDSA
                     ALAKAAIRRVRMDGGVLVADGIEVLAEMGPGCVRDFAEAACEVILIGSRGWDPLWSRR
                     PALCLDAPAIVTSLDDRLAFRLTPEQAERARQAARDKALAMAVPPDLALLSAGAREQN
                     AAALDRLALRIAPAVGWDDLVLPEALLVQLRELLGRVQHRHRVFSEWGMRRGEVKGLG
                     VTALFSGESGTGKTLAAEVVAGALGLDLYVIDLSTVVDKYIGETEKNLDRIFREADQV
                     NGVLLFDEADAIFGKRSEVQDARDRYANIEVSYLLQRMESFHGTAILTTNLRANIDKA
                     FMRRLDAVLEFPVPDVGMRRRIWEGHLPPGLPRDNSPDLEFLAARFDLAGGNIRNVAL
                     TAAFLAAAEDAPVSMAMLVKATAAEYRKLGRLCSEREFGPYFALVAPP"
BASE COUNT          692 a         1604 c         1644 g          667 t
ORIGIN      
        1 ctggtgctgc tcgaccggcg cccggcagcg ccggtccagg tgccccggcg caacccggcg
       61 ccatgcagcc cgaagccatg cagccggccg caccgaaggc ccggccctcg gccgccccgg
      121 ccaagccgac gaagcagctt cgcgagggcg atctgatctg cgggcagtgc ggtgagggca
      181 acgacgcgac ccgcaagttc tgcagccggt gcggcaacag cctggccacc gcggccaagg
      241 cccacatctc ctggtggcgc aagatcatcc cgcaccgggc cccgaagaag gcgctgaagg
      301 ccggcgagac caaggacaag cagaagaagg acaagctcag cagcaccatc gcgaccggac
      361 gcaagatctt cacctacggc gggatcgcca tcctgctcgg ggccgtcgcc atcagcctgc
      421 tgcagaaggc tgacccgtcg ttcaagaacc ccatcaccga caaggtggcc agcgcccggc
      481 actgggtcgg ccacgagtac acgaccctga agaacttcct cctgccgagc tacgagccgg
      541 tccactcgag cggcgacatc ccgacctcgg cgctgcccga ccatccgggc gtcctcgcca
      601 tcgacggctt caagaacacc tactgggccg cggacgggtc cagggacaag acgccgacgt
      661 tgggcgtgca cttcgacaag aaggtgacgc tcaagaagat gctgatcacg tcgggggact
      721 cggacaactt ccaggacgag ccccggccgc agcacatcca catcatctac tccaacaaca
      781 agacccagga cgtggtcctg gtcgacaagg ccgacccgca gacggtcacc ctgagcgggg
      841 ccaaggacat cacggacgtc catatccaga tcgtcgacac ctacacgtcc ctgaagggcc
      901 atgacgtttc gatcgccgag gtggagttct tccggctcaa gctctgagcg ggcggcgtcg
      961 ggctacgagc cgagcgactc gagcgtctct cgggccaccc gccgcagccc ctcgtcctgg
     1021 gcccgctcga cgtaggcgtc gagaagggac ttggtggcgg gggagcgcaa caagcgaagc
     1081 tcggcgagcg cctgccactg gatgtcgggg tcggggtcct gcaaggcccg ctggtacgcg
     1141 cccgcggcgt cgatcggtcc ccccgacggc aggttggcgc cgtcgaaggg catgaagaag
     1201 tacagctgct tgcacgccag catccgtatg gcgctcagcc cgctgcccag tccctcctcc
     1261 accttgggga aaccgatcgg gtctccgagc tgggccaggt agcccgcggc aaccagggcg
     1321 ctgaggtacg cgtccgccgg ccgcgagacc gcctcgacga gcgtctggcg ggcggctgcg
     1381 tcacccaggc ggcccaacgc gtaggccgcc tcgacggcgg cctggtcgtc ggagccctcg
     1441 accacctgcc ggagccgggg gatggcggcg ccgatggact ggctgccggc ggcgtgggcg
     1501 gccgcggcca gcaggacgcc ggtctggtcc tgcagggcgt gaacgatgcc gtccgccgtg
     1561 gtgggcaggt cgttggcctc gagcagccgc gccgtccggt agtcctcgaa ctcctcgtac
     1621 ggcgtgccgg ccggcgggtt gggcacgtcg aggttcgggc cgtcgaagga gatgtcctcg
     1681 agctgaaagg tcccttcgcc gtcccgctcg ccgtcgcctg ggctcacgta tagccgccgg
     1741 cggcaccctt ggtggtgatg gcgtcggcgc gggccttgta cgcgtccgcc ccgtccccgt
     1801 aggcccgctc ctccttggcc gggtacggca tggcggcctg ggacacggcg atcaccttgc
     1861 cgggcacctg cgtgagcagg ctctgcacgt cggcggcgct ggatgccgtc tgggacgcca
     1921 accaggtctg cgccgcggtg acgccctgcc cgccgaaggt ctggcgctcg gtggcgtgga
     1981 agttctcgtg gcgaaccgtc aggtccttgg cccagaactg ggtccgggga ggccggccgc
     2041 cgaggtcgcc catgttgggc gtcaggtcgc tggccgcagt ggcgtagttg gccgcggtca
     2101 gggcggagtc gctgtcggag gcgatgtccg tgtcgcctcc tccgtcgacg ttgaaggtga
     2161 tggggttctg caccgtggcg ctgaccttga aggtccccga gctggcggtc acgctgatcc
     2221 cggtgatgtt gaacttcgac catgtggtcg acccgaaggg gctgaccgtc ccgctctggg
     2281 cgaccgacgg cgcgtaggtg aggctgccgt cgacggcgtc ggtgtcgagg gccaggtctt
     2341 caggtgtctc gatgtcgggc aggctggccg tctggccctc ctggatgtcg gatgcctctc
     2401 ctcccgccgt tccgaccacg tcgtcaccgc tcagctcgct ggtgtcctcg gagcccggca
     2461 cggcgtcccg atcggcccgc ttgatggtgg cggtgaagct gagcgtcggc gctggcagga
     2521 cgtgctcctc ggcggcggtg gtggcggcgc cgactgttgc cgtcgtcgcg ccgtcggcgt
     2581 cgacggtcac cggcgctcgc tccaggagcg agctcaccgc ggcgttgccg gcgatgtgct
     2641 ggagccgggc catgtcggcc tgcgccaggt gctgatcccg gtctcccggt gcgagccggg
     2701 cgagcagggc cgacgcctgc ctggcggcat cgggcgcgct acccggcgga cgggtccgcc
     2761 gtgagctccg aagtgggtcg ctgctgtcgc ccgaacggtg ccagtcggtc atccgtgggt
     2821 cctcctcgac gtccgccccc acggccgaga tgtgtgggga aaccatctag agcatagacc
     2881 tctggggccg gtcgcggccg cccgttccca ggtctctcga gagtttccca agtggcctac
     2941 ggcggcgcca ccagggcgaa gtagggcccg aactcacgct ccgagcacag gcgcccgagc
     3001 ttgcggtact cagcggccgt agccttcacg agcatcgcca tcgagaccgg cgcatcctcg
     3061 gcggcggcca ggaatgccgc cgtgagggcc acgttgcgga tgttgcctcc tgcgaggtcg
     3121 aaccgggcag ccaggaactc gaggtccggc gagttgtcgc gcggcaggcc cggggggagg
     3181 tgcccctccc agatccgccg ccgcatcccg acatcgggca ccgggaactc cagcaccgcg
     3241 tccagccggc gcatgaaggc cttgtcgatg ttggcccgaa ggttggtggt caggatggcc
     3301 gtgccgtgga agctctccat gcgctggagc aggtaggaca cctcgatgtt ggcgtagcga
     3361 tcgcgagcat cctggacctc cgagcgcttg ccgaagatgg cgtcggcctc gtcgaagagg
     3421 agcactccgt tgacctgatc ggcctcgcgg aagatgcggt cgaggttctt ctccgtctcg
     3481 ccgatgtact tgtcgacgac ggtcgacagg tcgatcacat agagatcgag gcccagagcg
     3541 ccggcgacga cctcggcggc cagcgtcttg cccgtgccgg actccccgga gaagagcgcc
     3601 gtcaccccga ggcccttgac ctcgccccgt ctcatccccc actcggaaaa gacccgatgg
     3661 cggtgctgca cgcggccgag cagctcgcgc agttgcacga gcagagcttc cgggagcacc
     3721 aggtcgtccc atccgacggc aggtgcgatc cgaagggcga gccggtccag agcggccgca
     3781 ttctgctcac gcgcgccggc ggagagcaga gccaggtcgg gcgggaccgc cattgccagc
     3841 gccttgtccc gggcggcctg gcgcgcccgc tcggcctgct cgggagtgag ccggaaggcc
     3901 aggcgatcgt cgagcgaggt gacgatcgcc ggagcgtcca gacacagcgc cgggcgccgc
     3961 gaccagaggg ggtcccaccc gcgactgccg atgaggatca cctcgcaggc ggcctcggcg
     4021 aagtcccgga cgcagcccgg ccccatctcg gcgaggacct cgatgccgtc ggccacgagg
     4081 acgccgccgt ccattcgaac ccggcgaatc gcggccttgg ccaacgccgc gctgtcctcg
     4141 cctgtggtca ggcgccgcag gtcgagggcc aggaggggcc gcccagcagc ccggaggctc
     4201 tgtgccgcgt aggagagcgc ggcggtgcca ggatggtccc ggaggtagac cagccgcacg
     4261 ccgccctcga gggcccgggt gatcggcgca gtgtcgtagc ccggcaccgg taccgccgtg
     4321 gtgactatcg gggcgacgac cggatcggcg acgtcgtcgc cgaggaggtg gccggccacg
     4381 atgtccggga cgcggagcga ccgcgtgagg aagggtcgct cgccgtcctc gatcatgagc
     4441 agcccgcccg ccaccaaggg ggcgtccggc gacaaccgcg cccggctggc cgggtgcacg
     4501 agatcaccgc cgcacagctc gatggcgagc cccacgctgg ctcggcgcct gctgacgtcg
     4561 tcgtgcaggt agccgtaggc gcgctccaac ctgggatcga gatccgg
//
LOCUS       DASWGG010000301         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1888155, whole genome shotgun sequence.
ACCESSION   DASWGG010000301 DASWGG010000000
VERSION     DASWGG010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888155"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..1617
                     /locus_tag="VF954_05390"
     CDS             385..1617
                     /locus_tag="VF954_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX9530550.1"
                     /translation="MRPVRGQLAWLAHGLIGPDTASARQGFYALLISSGGDLLAGVTL
                     GSIQHTLEQLPGLLVLIPAAIGMRGNIFGALGSRLGTAIHTGTFTLSRRRSTIVGQNV
                     LSSLTLTFVVSLALAALAKSIAVAFNLRSISLLDFVVISMVGGVISSAFVLAITVGVA
                     AGSARYGWDMDSVAAPIITAAGDMVTLPSLFLATYCVGVRFLTPFLGALTAGLAAVSV
                     VGVLRSGLPLLRRIVAESLPILLIAGAIDIIAGISIEKRLAAFTTLPALLVLVPPFLE
                     DTGALGGILASRLSSKLHLGIIEPARWPQRAARADFRLVAVFAIPVFTLVAISADVVS
                     LFLGLGSPGPVRMIAISLIGGLLATSACLAITYYGAIAAYRLGLDPDNHGIPLVTSSM
                     DLIGAVALIFAIVIIRVG"
BASE COUNT          201 a          624 c          538 g          297 t
ORIGIN      
        1 tcgaggtcgt gtcggtgccg aggcccggat cggggctccc gacgtccgct acggcgtggg
       61 cggcggggag gctgaccaga gccatgaaca tggtggtcag ggccagggct cgcccaactc
      121 gcctcaacaa cactcggatc tccttcccgg ggttcggggt gtcccccgga ctgacagcga
      181 ctcgaacggc atcgctgtgg agcacatcgg tgcgtagtgg tcgttcgtca cgaaacggaa
      241 tgttccaaac agatgacggc gccgggagca ctccgctcgc cggttggcgg gtctgcccgg
      301 tcagcccggg ggttcggggg tgtcccccgt agataaactc ctcgcccgtg atcgccgtcc
      361 agccgctgca ggagcctccg agtcgtgcgc cccgtacggg ggcagttggc gtggctggcg
      421 cacggcctga tcgggcccga cacggccagc gcccggcagg gcttctacgc cctgctcatc
      481 tcgtcgggcg gtgatctcct cgccggtgtg accctcggga gcatccagca cacgctggag
      541 cagctccccg gcctgctggt gctcatcccg gccgccatcg gcatgcgggg caacatcttc
      601 ggggcgctcg gcagccggct cggcaccgcc atccacaccg gcacgttcac cctgtcgcgg
      661 cgccgctcca ccatcgtcgg ccagaacgtg ctgtcgtccc tcacgctgac attcgtcgtg
      721 tccctggcgc tggcggcgct ggccaagtcg atcgccgtgg cgttcaatct ccgctcgatc
      781 tcgttgctgg acttcgtcgt catctccatg gtgggcggcg tgatctcctc ggccttcgtc
      841 ctggccatca cggtgggcgt ggccgccgga tcggcgcgct acgggtggga catggacagc
      901 gtggccgccc cgatcatcac cgccgccggc gacatggtga cgctcccgtc cctgttcctg
      961 gccacctact gcgtgggggt ccggttcctc accccctttc tcggcgccct caccgccgga
     1021 ctggccgccg tcagcgtggt cggcgtgctg cggtcgggcc tgccgctcct gcgaaggatc
     1081 gtggccgagt cactgcccat cctgctcatc gccggggcga tcgacatcat cgccggcatc
     1141 agcatcgaga agcgcctggc ggcgttcacc accctgccgg cgctgctggt gctggtaccc
     1201 ccgttcctgg aggacaccgg cgccctcggc gggatcctgg cctcccgcct gtcgtcgaag
     1261 ctccacctcg gcatcatcga gccggcccgc tggccgcagc gggccgcccg ggccgacttc
     1321 cggctcgtgg ccgtcttcgc cattcccgtc ttcaccctgg tggcgatctc ggccgacgtc
     1381 gtgagcctgt tcctcggcct ggggagcccc gggcccgtcc gcatgatcgc catctccctc
     1441 atcggcggtc tgctggccac gtccgcctgc ctggccatca cctactacgg cgccatcgcc
     1501 gcctaccgcc tcggcctcga ccccgacaac cacgggatcc cgctggtcac ctcgtcgatg
     1561 gacctgatcg gtgccgtggc cctgatcttc gccatcgtca tcattcgggt gggctagaag
     1621 cggtcgcccc tcagcaccac ccggcggcca ggaccggagc
//
LOCUS       DASWGG010000302         7210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_127847, whole genome shotgun sequence.
ACCESSION   DASWGG010000302 DASWGG010000000
VERSION     DASWGG010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127847"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..979
                     /locus_tag="VF954_05395"
     CDS             35..979
                     /locus_tag="VF954_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9530551.1"
                     /translation="MVSLGVHLGQQNLAMDELRALWRRCDASMDWISAWDHFYEAPPQ
                     GGTVPHFEAVATLAALACETSRARIGCLVFYVGYRNPAQLAKVATTLDHLSNGRFELG
                     LGAGWHAWEAAAYGYDFPPAGRRLDMLEEAAPLIRRLLTEERTTHRGEWFRTEDASCL
                     PPPVQPRLPIWIGGVGEKRTLRIAAREADGWNAAYVPPEEFRRLNGVLDEWCEREGRD
                     PSTVRRTVNLQFMLAVDDRSADRVRAQVSGQWGALAPRVMAGGLTGVPETAVERIAEY
                     VDAGADGINIALRAPWPPETLDAWFDEVVPSVRAETHS"
     gene            1066..1497
                     /locus_tag="VF954_05400"
     CDS             1066..1497
                     /locus_tag="VF954_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9530552.1"
                     /translation="MAKTNEKLDRLATFELFRHVARKDLPRVADMVGNEMTVPAGHVL
                     TEQGRVAEDAYLVVDGTADVIVGDRSVAEIGPGESVGEMAVVDNWPRSAYVVARTPMK
                     VYRLSGPTFLKLLDEVPTVARALIESLSVRLRRLDVRPPGF"
     gene            complement(1739..3007)
                     /locus_tag="VF954_05405"
     CDS             complement(1739..3007)
                     /locus_tag="VF954_05405"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9530553.1"
                     /translation="MSRLASAGANTFRSLRVRNYKLFFFGQLVSVCGTWMQTIAQAWL
                     VLRLSHDSGVAVGVVTALQFTPTLLFGMWGGVIADRFDKRRLLVGTQLAMTAAAAALA
                     TLTLTGAVQLWHVYALALVTGLATAVNAPAHQSFVVEMVGPQDVGNAVGLNSAGFNAG
                     RIVGPALAGLVIVTVGTGWCFAVNAASFVAAIGGLMAMRPGELWRGAPVARAKGQVRE
                     GFRYVRRTPELRSNVILLTIVGTLAYNFQVVIPILAKVTFHGSAGTYSAMTVAMGSGS
                     LVGALLAASRRRPSGELLFGASLAFGAANCLAAVSPNLVTVLPALVLLGLCSITYVST
                     ANTLLQLRSDAEMRGRVMAIYALVVIGSTPIGGPLLGWICQQWGARWGLGLGGLSTLA
                     GAVFFGTSLLRQRRLGGGMDRDLVGEPAAA"
     gene            complement(3004..3477)
                     /locus_tag="VF954_05410"
     CDS             complement(3004..3477)
                     /locus_tag="VF954_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9530554.1"
                     /translation="MSQATTPHAATPGLGTSQLASRLRIAVARLSRRLRQQTPEAHGD
                     ITASQLAALGSIERLGPISLGELASIEQVQPPSMTRIVGRLEDLGFASRLVDPTDRRV
                     ARASATDAGRRFLARARTRKDTYLSRRIDRLEPAERDQLLRGIEILERLLGGDEA"
     gene            complement(3541..4785)
                     /locus_tag="VF954_05415"
     CDS             complement(3541..4785)
                     /locus_tag="VF954_05415"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX9530555.1"
                     /translation="MAGDPWGAVLERLDDAAKLTGIDPDVHRMLRAPRRVLEVSVPVR
                     MDDGRVEVFTGWRIHHDTTRGPAKGGIRFHPDLDAREVAALAASMTFKTAVANLPFGG
                     GKGGVRCDPGRLSLGELERLTRRWTVEVSPLLGPDRDIPAPDVNTDGRVMAWLMDTLS
                     MMRGADLPGSVTGKPLSIGGTRGHAGATSTGVLICARAAFKELDMGFAGGRVVIQGFG
                     KVGGPLAFLLSSAGMRVIAVSDVGGAVHNPAGLDIPALADHVAHAGSVANFERAESVD
                     ADGIWDLECELVVPAALSGVIDSHVAQRMGGKVIVEAANGPTLPEADSILDRRDIVVV
                     PDILANVGGVTASYFEWAQSRQGYAWDDDVVASRLNKVMESAFMDVWAKSDALSVSLR
                     RAAFSLALERVADAISARGLFP"
     gene            complement(4828..5136)
                     /locus_tag="VF954_05420"
     CDS             complement(4828..5136)
                     /locus_tag="VF954_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530556.1"
                     /translation="MGQPIVVVEKPSSKPGVVRFEINRSLTGMAHERYRSPDDISEDR
                     PPDRLARLLFSRGGIDGVHIYSNVITVDLAKGATSDGLADLIRELYIHYREGVTPSLP
                     "
     gene            5240..6340
                     /locus_tag="VF954_05425"
     CDS             5240..6340
                     /locus_tag="VF954_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9530557.1"
                     /translation="MAAPASQVPARTVPVRTALTVIAVGLATVGALEMLLQLRHIVLW
                     VAIAAVLAVGLAPPVTRVQRWLHLPRAAATLLVFVVVSALAFGAAYVFFRPLVAELQD
                     FINHFPRYVSDARAGRGPVGSLVKHYKLDAWVDRNQDKVQAALKSAEKPALAAAKGLV
                     NTVAALVTIVVLAFLLILEGPRMVENAINALPDASRSRVRAVVKVSARSVSGYLGGTV
                     AISFIAGAVIYLFLLATGVPYRGALALWMGFADLVPIVGVTIGAIPVVVFAFLNSTPI
                     GIGAVAMVLVYQVVEVRFLRRLAFSHSMRLNPLAVAVGILAGLQLFGLLGAVLALPVV
                     SVIKVLGRALVEWRRPDLVEAGLARPVELAEE"
     gene            complement(6362..6757)
                     /locus_tag="VF954_05430"
     CDS             complement(6362..6757)
                     /locus_tag="VF954_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9530558.1"
                     /translation="MLERTSGDLTLPQYRVLAMVARGDERASHLADRLALAKPTITAV
                     VDGLAERGLLSRSTVDEDRRAARIQVTADGRKALLAAEAAMGERVQSLLDRTTDPPAV
                     LAALVDLGRALDDASAERMDAMGAARPAV"
     gene            complement(6735..7049)
                     /locus_tag="VF954_05435"
     CDS             complement(6735..7049)
                     /locus_tag="VF954_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530559.1"
                     /translation="MSRRHANLLRASAGWALWVWAVLIRNMVVDHTHSLGFRAVHVGL
                     AVVSLGFAAATLWVASRGRRAARSGAAVNQSIGILQLREGPVARRPDPGPPGAGARTH
                     LR"
BASE COUNT         1050 a         2558 c         2597 g         1005 t
ORIGIN      
        1 cgttcgcacg cgtcctgctc ctatcatcgg cgcggtggta tcgctcggcg ttcacctcgg
       61 tcaacaaaac ctcgccatgg acgagctgcg cgccctgtgg cgccgctgcg acgcgtcgat
      121 ggactggatc tccgcctggg atcacttcta cgaggcgccc ccgcagggcg gcaccgtccc
      181 gcacttcgag gcggtggcga cgctcgccgc cctggcgtgc gagaccagcc gggcccgcat
      241 cggctgcctc gtgttctacg tcggataccg caacccggcg cagctggcca aggtggcgac
      301 caccctggac cacttgagca acgggcgctt cgagctcggg ctcggcgccg gttggcacgc
      361 gtgggaagcc gccgcgtacg gctacgactt cccgccggcg ggccggcgcc tggacatgct
      421 cgaggaagcc gcgccgctca tccggcggct cctgaccgag gaacgcacga cccaccgggg
      481 ggaatggttc cgcaccgagg acgcgtcctg cctgccgccg ccggtgcaac cccggcttcc
      541 catctggatc ggcggcgtcg gggagaagcg cacgctacgg atcgcggcac gggaggccga
      601 cggatggaat gccgcgtacg tgcccccgga ggagttccgc aggctcaacg gcgtgctgga
      661 cgagtggtgc gagcgcgagg gccgggaccc ctctaccgtg cggcgtacgg tcaaccttca
      721 gttcatgctg gcagtcgacg accgctcggc tgatcgtgtc cgagcccagg tgagcggcca
      781 gtggggcgcc ctggcacctc gcgtgatggc aggtggtctc accggggtcc ccgaaacggc
      841 ggtggaacgc atcgccgagt acgtcgacgc cggcgccgac gggatcaaca tcgccctccg
      901 cgcgccatgg cccccggaga cgctggacgc gtggttcgac gaggtcgtgc ccagcgtccg
      961 ggctgaaacg cattcctgag gacgaccgcc gaagagcaga cggctcgcgg gcgcggacct
     1021 agcggtacat tccttcggag cgcggcagag cgacgaggga gatgcatggc caagaccaac
     1081 gagaagctgg accggctggc gacgttcgag ctcttccgcc acgtggccag gaaggacctc
     1141 ccccgcgtcg ccgacatggt gggcaacgag atgaccgtcc ctgccggtca tgtgctcacg
     1201 gagcagggcc gggtggccga ggacgcctac ctggtggtcg acgggacggc cgacgtgatc
     1261 gtgggcgacc ggtcggtggc cgagatcggc cccggcgagt cagtcggcga gatggccgtg
     1321 gtcgacaact ggccccgatc ggcctacgtg gtcgcccgca ccccgatgaa ggtgtaccgc
     1381 ctgtcgggcc cgacgttcct caagctgctg gacgaggtgc cgacagtggc ccgcgccctg
     1441 atcgagtccc tctcggtccg gctgcgccgg ctcgacgttc gcccgcctgg gttctgatct
     1501 ggtctgagaa gcggcgcgct tgcagtcccg accaggcccg gtccggtggg caacccactc
     1561 gtggaccggg cccgatcggc gtgtccggag agactgggga ctccaggcac ccgttccccc
     1621 gcccgagggg atcggctcag gcgggcgacc gtcagtgggg gaacgggcgg ggtggtggca
     1681 ccttgcggtc gcccgcggga tttccgccgg cggcagagcg tgcaggaggc ccctcggctc
     1741 aggccgcggc cggctccccc accaggtccc ggtccatccc gcccccgagc cggcgctgcc
     1801 ggagaagcga ggtgccgaag aacacggcac ccgccagggt cgagaggccg ccgaggccga
     1861 ggccccacct ggcgccccat tgctggcaga tccaacccaa cagcgggcca ccgatcggcg
     1921 tcgacccgat gaccacgagg gcgtagatgg ccatcacccg accgcgcatc tcggcatcgg
     1981 accgcagctg cagcaaggtg ttggcggtcg agacgtacgt gatggagcag agcccgagca
     2041 ggaccaacgc cgggagcacg gtgacgaggt tgggcgatac cgccgccagg cagttggcgg
     2101 cgccgaaggc gaggctggca ccgaagagca gctcgcccga gggccgccgg cggctggccg
     2161 cgaggagggc tcccaccaga gaccccgatc ccatggcgac ggtcatggcg ctgtatgtgc
     2221 cggccgagcc gtggaaggtc accttggcca ggatggggat cacgacctgg aagttgtaag
     2281 ccagcgtccc gacgatggtc aggaggatca cgttcgagcg cagctcgggg gtccgccgca
     2341 cgtaccggaa gccctcacgc acctggccct tggcgcgcgc caccggagcg ccccgccaca
     2401 gctcgcccgg gcgcatggcc atcaggccgc cgatggcggc gacgaaggat gcggcgttca
     2461 cggcgaagca ccatcccgta ccgaccgtca cgatgaccag gccggcgagg gccggcccga
     2521 cgatccggcc cgcgttgaag ccggcgctgt tgagccccac ggcattgccc acgtcctgcg
     2581 gaccgaccat ctcgaccacg aagctctggt gggccggggc gttgaccgcg gtggccaacc
     2641 cggtcaccag ggccagcgcg tagacgtgcc acagctggac cgcgccggtg agcgtgaggg
     2701 tggcaagggc ggccgcggcc gcggtcatgg ccaactgggt cccgaccagc agccggcgct
     2761 tgtcgaagcg gtcggcgatc acgccgcccc acatcccgaa cagcagcgtg ggcgtgaact
     2821 ggagggcggt caccacgccg actgcgacgc cgctgtcgtg ggacagcctg aggaccagcc
     2881 acgcctgggc gatcgtctgc atccaggtgc cgcagacgga tacgagctgg ccgaagaaga
     2941 acagcttgta gttgcggacc cggagggagc ggaacgtgtt ggccccggcc gacgccagcc
     3001 ggctcacgcc tcgtcccccc cgagcagccg ctcgaggatc tcgatgcccc ggagcagctg
     3061 gtcgcgttcg gccggctcca gccggtcgat gcggcgcgac aggtaggtgt ccttccgggt
     3121 gcgcgcccgg gccaggaacc ggcgcccggc atcggtggcc gacgctcggg cgacccgccg
     3181 gtcggtggga tcgacgaggc ggctggcaaa gccgagatcc tcgagacgtc ccacgatgcg
     3241 cgtcatgctc ggcggctgca cctgctcgat cgaggccagc tcgcccagcg agatgggccc
     3301 cagacgctcg atggacccga gggcggccag ctgagatgcg gtgatgtcgc cgtgggcctc
     3361 gggggtctgc tgccggagcc tccgggacag ccgggccacc gcgatccgca ggcgcgaggc
     3421 cagctgagac gtgccgagac ctggggtggc ggcgtgggga gtcgtggcct ggctcatgtt
     3481 tacctcaact aattagctga ggtaattatt ccacgtccgg gccggcgaga caacccgggc
     3541 tcacgggaac aggccccggg ccgagatggc gtcggctacc cgttcgaggg ccagtgagaa
     3601 ggcggcccgg cgcaaggaca cgctgagggc gtcggacttg gcccacacgt ccatgaaggc
     3661 gctctccatg accttgttga gccggctggc caccacgtcg tcgtcccacg catagccctg
     3721 ccggctctgg gcccactcga agtaggaggc cgtgaccccg ccgacgttgg cgaggatgtc
     3781 cggcacgacc acgatgtcgc gccgatccag gatgctgtcc gcttccggga gggtaggccc
     3841 gttggccgcc tctacgatca ccttgccgcc catccgctgg gccacatggc tgtcgatgac
     3901 accggacagg gcggccggga cgaccagctc gcactccagg tcccagatcc cgtcggcatc
     3961 gacgctctcg gcccgctcga agttggccac cgagccggca tgggcgacgt ggtcggccag
     4021 agccggtatg tcgaggccgg cggggttgtg aacagcccca ccgacgtcgc tgacggcgat
     4081 gacgcgcata ccggccgagc tcagcaggaa ggcgagcggt ccccccacct tgccgaagcc
     4141 ctggatgacc acgcgtccgc ccgcgaaacc catgtcgagc tccttgaacg cggcccgggc
     4201 gcagatcagc actccggtgg acgtggcccc ggcgtggccg cgggtgccgc cgatggacag
     4261 cggcttgccc gtgaccgacc ccggcaggtc ggctccccgc atcatcgaca gcgtgtccat
     4321 cagccaggcc atcacccggc cgtcggtgtt gacgtccggg gccgggatgt cccggtccgg
     4381 gcccagcaga ggcgacacct ccacggtcca ccggcgcgtc agccgctcca gctcgccgag
     4441 agagaggcgg cccggatcgc agcgcacgcc acccttgccc ccaccgaagg gaaggttggc
     4501 caccgccgtc ttgaacgtca tgctggcggc cagggcggcc acctcccggg cgtcgaggtc
     4561 ggggtggaag cggatcccgc ccttggccgg gcccctggtg gtgtcgtggt ggatccgcca
     4621 acccgtgaac acctcgaccc gtccgtcgtc catccggacc gggaccgaca cctcgagcac
     4681 ccggcgcggc gcccggagca tcctgtgcac gtccgggtcg atgccggtga gcttggcggc
     4741 gtcatccagc cgctcgagca ctgcccccca cggatctcca gccatcgcct ccccctcccg
     4801 actccctgtg gctcaggggt gatgggctca gggtaacgac ggtgtcacgc cctcgcggta
     4861 atggatgtag agctcccgga tcagatccgc caggccgtcg ctcgtggccc ccttggccag
     4921 gtccacggtg atgacgttgg agtagatgtg cacgccgtcg atgccgccgc gggagaacag
     4981 gagccgggcc agacggtcag gtggacgatc ctcgctgatg tcgtccggcg agcggtaccg
     5041 ctcatgggcc atgcccgtga ggctccggtt gatctcgaag cggacgacac ctggcttgga
     5101 cgacggcttc tcgacgacga cgatcggctg acccacgagg gcacgaggct acgcccggga
     5161 gcccgtgccc cgccaccacc ctccaccacc tcgctcggtg ccacgggcag gcggtcggga
     5221 catgggtagg ctggcggcaa tggccgcccc agcgtctcag gtgccggccc gaacggtccc
     5281 cgtgcggacc gcgctgacgg tcatcgccgt cgggctggcc acggtaggcg ccctggagat
     5341 gctgctccag ctccgccaca tcgtgctgtg ggtcgccatc gcggccgtgc tggccgtcgg
     5401 gttggcgccg ccggtcacga gggtgcagcg ctggctccac ctgccgcggg cggccgccac
     5461 cctcctcgtc ttcgtggtcg tctccgccct ggccttcggc gccgcgtatg tcttcttccg
     5521 gccgctcgtc gccgagctgc aggatttcat caaccacttc ccgcgctacg tgagcgacgc
     5581 ccgggccggc cggggcccgg tcgggtcgct cgtcaagcac tacaagctcg acgcctgggt
     5641 ggaccgcaac caggacaagg tgcaggccgc gctcaagtcg gccgagaagc ccgccctggc
     5701 cgcggccaag ggcctggtca acacggtggc cgcgctggtc acgatcgtcg tgctcgcgtt
     5761 cctcctgatc ctggaggggc ccaggatggt cgagaacgcc atcaacgcgc tgcccgacgc
     5821 cagccggagc cgggtgcggg ccgtcgtgaa ggtcagcgcc cgcagcgtgt cgggctacct
     5881 cggcggcacc gtcgccatca gcttcatcgc cggcgcggtc atctacctgt tcctgctcgc
     5941 caccggcgtg ccctaccggg gagcgctggc gctgtggatg ggcttcgccg acctggtgcc
     6001 catcgtcggt gtgaccatcg gcgccatccc cgtggtcgta ttcgccttcc tcaactccac
     6061 gcccatcggg atcggcgccg tcgccatggt gctcgtgtac caggtggtcg aggtccggtt
     6121 cctgcgccgg ctggcgttct cccactcgat gcggctcaac cccctggccg tggcggtcgg
     6181 gatcctggcc gggctccagc tcttcggcct gctcggagcc gtcctcgccc tgcctgtcgt
     6241 gagcgtgatc aaggtcctgg gccgggccct ggtcgagtgg cgccgcccgg acctggtcga
     6301 ggccgggttg gcccggcccg tggagctggc cgaggagtga tcgctggttg cggcctcggg
     6361 ctcagaccgc cgggcgggcg gcgcccatgg cgtccatgcg ctcggctgac gcgtcgtcca
     6421 gagcccgccc gaggtcgacc agcgcggcga gcacggcggg gggatcggtg gtccggtcga
     6481 ggagggactg gacccgctcg cccatggccg cctcggcggc gaggagagcc ttgcgaccat
     6541 cggccgtgac ctggatgcgc gccgctcggc ggtcttcgtc gacggtggaa cgcgagagca
     6601 ggccgcgttc ggccaggccg tccaccaccg ccgttatggt gggcttggcg agcgccaggc
     6661 ggtcggcgag atgggaagcc cgctcgtcgc cgcgcgccac catggccagg acccggtact
     6721 ggggcagggt caggtcaccg gaggtgcgtt cgagcacccg cgccaggcgg gccagggtcc
     6781 ggacggcggg cgacaggtcc ttcacgcagc tgcaggatac cgattgactg gttgaccgcg
     6841 gcgccggagc gcgccgcccg gcggcccctg ctggccaccc acagcgtggc cgccgcgaag
     6901 ccgagcgaga cgaccgccag gccgacgtgc acggcccgga agccgaggga gtgggtgtgg
     6961 tcgacgacca tgttccggat gaggacggcc cagacccaca gggcccaccc ggccgaggcc
     7021 cgcaggaggt tggcgtggcg gcggctcacc ggcccgaacc taccgcggcg gcctggagac
     7081 cccacgtcgc caggacctgt tggtacgtgc cgtcggcttc gatcgaggcc agcgcggcct
     7141 gcagggccgg gcgaaggccg gcgctggtca cgatcagccc gacgggctcg gcgtcgacgg
     7201 ggccgccgag
//
LOCUS       DASWGG010000303         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1888882, whole genome shotgun sequence.
ACCESSION   DASWGG010000303 DASWGG010000000
VERSION     DASWGG010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888882"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1097
                     /locus_tag="VF954_05440"
     CDS             144..1097
                     /locus_tag="VF954_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9530560.1"
                     /translation="MKTVTGEDVTLEQLGGATSHATKSGVATFVAADEKSCLDEVRYL
                     LTFLPSNNLEEPPWVAPGDDPERRCADLVDIIPASANQPYDMKRVVASIVDDGEYFEY
                     FPNWAMSLTCGFARLNGHVVGIVGNQPAVLAGVLDIESSEKGARFVRTCDAFNIPLVT
                     FVDVPGYMPGTDQEYGGIIRHGAKILYAYCESTVPRIQVITRKAYGGAYVVMNSKSIG
                     ADLAFAWPSAELAVMGPQGAVEIVYRRELAEAVDPVARRNELVEEYTERYANPYVAAE
                     RGYVDDVIDPAETRAVLIRSLEMLRTKREELPKRKHGNVPL"
     gene            1084..1272
                     /locus_tag="VF954_05445"
     CDS             1084..1272
                     /locus_tag="VF954_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530561.1"
                     /translation="MSPSDRLAPDDPAVVAAIAAAVEVVWPRPAAAAQPDTRPAWRFA
                     GRWWARPVAARRDRPWTS"
     gene            1309..2364
                     /locus_tag="VF954_05450"
     CDS             1309..2364
                     /locus_tag="VF954_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9530562.1"
                     /translation="MIFDDGPPVLELTTVADDHVVLHVPAGADEPGSPARALRFYGLQ
                     PDRDYTLEGVEVRTLPRPPGERLATFATVNDVHFGETECGVVEGTEVGPILSSAAGED
                     PYPETMNRAAVAEILALGPDAVVVKGDLTSRGTDGEFEAFLACYGAAFGDRLHWVRGN
                     HDVAHGDHFAGKAPFAVQLPGVTLAVFDSAVPGQAGGRVPAETLTWLDQVAASAGHPV
                     LMFAHHHPWSPESSARSSTYFGINPDDSERLVEVVARQAHIVGYFAGHTHRNRVRHFA
                     ATGRVPWAEVACVKDFPGTWAEYRVFEGGVLQVHHRISSPEALAWSERTRVLYAGLYP
                     EYSFGRLEDRCFAIPLR"
BASE COUNT          370 a          967 c          853 g          373 t
ORIGIN      
        1 accaagtccg gggtggccaa cttcaccgcg gcggacgacc gcgcctgcct cgacgacgtc
       61 cgctacctgc tcagcttcct gccctccaac aacctcgagg agccaccccc ggtcgagacc
      121 gcggacgacc cggcccggac gtggtgaaga ccgtcaccgg cgaggacgtc acgctcgagc
      181 agctgggcgg cgcgaccagc cacgccacca agagcggcgt cgccaccttc gtggccgcgg
      241 acgagaaatc ctgcttggac gaggtgcggt acctgctgac ctttctcccg tcgaacaacc
      301 tggaggagcc tccgtgggta gcccccggcg acgaccccga gcgccggtgc gcggacctgg
      361 tcgacatcat cccggccagc gccaaccagc cctacgacat gaagcgggtg gtcgcctcca
      421 tcgtcgacga cggcgagtac ttcgagtact tccccaactg ggccatgagc ctcacctgcg
      481 gcttcgcccg gctcaacggg cacgtggtcg gcatcgtcgg caaccagccc gccgtgctgg
      541 ccggcgtgct cgacatcgag agctccgaga agggcgcccg gttcgtgcgc acctgcgacg
      601 ccttcaacat ccccctcgtc accttcgtgg acgtgcccgg ctacatgccc ggcaccgacc
      661 aggagtacgg cggcatcatc cgccacggcg ccaagatcct gtacgcctac tgcgagtcga
      721 ccgtcccccg catccaggtc atcacccgca aggcctacgg cggcgcctac gtggtcatga
      781 actccaagtc catcggcgcc gacctggcct tcgcctggcc gtccgccgag ctggccgtga
      841 tgggacccca gggcgcggtc gagatcgtct accgccgcga gctggccgag gcggtcgacc
      901 cggtggcccg gcgcaacgag ctggtggagg agtacaccga gcgctacgcc aacccctacg
      961 tggccgccga gcggggctac gtcgacgacg tcatcgaccc ggccgagacc cgggcggtgc
     1021 tgatccgcag cctggagatg ctgcgcacca agcgggagga gctccccaag cgcaagcacg
     1081 gcaatgtccc cctctgaccg gctggccccc gacgaccccg ctgtggtggc cgccatcgcc
     1141 gcggccgtgg aggtggtgtg gccccgcccg gccgccgccg cgcagcccga cacccgcccg
     1201 gcgtggcgct tcgccggccg gtggtgggcc cggcccgtcg ccgcccggcg cgaccggccc
     1261 tggacgtcct gaatgacctg gacgctctga atgacctgga cgtcctgaat gatcttcgac
     1321 gacggtcccc ccgtcctcga gctcacgacc gtcgccgacg accacgtcgt gctccacgtc
     1381 ccggcggggg ccgacgagcc cggcagcccg gcccgggcgc tccgcttcta cggcctccag
     1441 cccgaccggg actacacgct cgagggcgtg gaggtccgga ccctgccccg cccgcccggc
     1501 gagcgcctgg ccaccttcgc gacggtcaac gacgtgcatt tcggcgagac cgagtgcggt
     1561 gtcgtcgagg gcacggaggt gggccccatc ctgtcgagcg cggccggcga ggacccctac
     1621 cccgagacca tgaaccgcgc cgccgtggcc gagatcctcg cgctggggcc ggacgcggtg
     1681 gtggtgaagg gcgacctgac ctcacggggc accgacggtg agttcgaggc cttcctggcc
     1741 tgctacgggg cggccttcgg cgaccggctg cactgggtga ggggcaacca cgacgtggcc
     1801 cacggcgacc atttcgccgg gaaggccccg ttcgccgtgc agctgccggg cgtgacgctg
     1861 gcggtgttcg actccgccgt gccgggccag gccggcgggc gggtacccgc cgagaccctg
     1921 acctggttgg accaggtagc cgcgtccgcc ggccatcccg tgctgatgtt cgcccatcac
     1981 cacccgtgga gccccgagtc cagcgcgcgc agctcgacgt acttcggcat caaccccgat
     2041 gactccgagc gcctggtgga ggtcgtggcg cggcaggcgc acatcgtcgg ctacttcgcc
     2101 ggtcacaccc atcgcaaccg ggtgcgccac ttcgccgcca cgggccgtgt gccctgggcc
     2161 gaggtggcgt gcgtgaagga cttccccggc acctgggccg aataccgggt cttcgagggc
     2221 ggcgtgctcc aggtccatca ccgcatctcc agccccgagg cgctggcgtg gtccgagcgc
     2281 acccgggtgc tgtacgccgg tctctacccg gagtattcct tcggccggct ggaagaccgc
     2341 tgcttcgcaa tcccgctgcg ctgatccccg aggccgaccc cctgaggccg accccctgag
     2401 gctgaccccg cccaacctgg ggataacggt gttccagttc ggtgaccagt acgttctggg
     2461 tgccgcgtcc tggctcgatt tcatgtcccg tacatctagg actagtcacc acaggcgctt
     2521 gaccggcccg tttgctatgg gtcgggccgg ttcaggggtg cta
//
LOCUS       DASWGG010000304         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3173930, whole genome shotgun sequence.
ACCESSION   DASWGG010000304 DASWGG010000000
VERSION     DASWGG010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3173930"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1643)
                     /locus_tag="VF954_05455"
     CDS             complement(<1..1643)
                     /locus_tag="VF954_05455"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEX9530563.1"
                     /translation="MRGGLHTFGRPPEGDAEIDLLAALLRLPQGSVPSLRSAVAHGLG
                     LDLRELLAEPGRRLDPAETGDHAWTTASDAVDTIDAEAKRLLRAGRDAAWPAEPAFLC
                     RDTAEIERQKPGAAGAAATGVPLPESSGNRTPDAEATEPAFISAAGPRSGTPVGGRHG
                     GDGGVGEVGRALRFAGDVLVPNLAATTDEVTNTLRALDGRYVPAGPSGAPTRGMAHVL
                     PTGRNFYSVDPKTLPTKAAWDVGRALADAVCARHRQETGKWPASVGLVVWGTAAMRTH
                     GDDIGEALALLGARPVWAEESGRVTGVEPVPLEELGRPRIDVTLRISGFFRDAFPHLV
                     HLVDEAVELVRHLDEPPEQNFVRAHGDDHRIFGAKPGAYGSGILALLDSKDWESDDDL
                     ATVYLAWSGYAYGRQGYGVPAPEAMQRRFALIDVAVKNQDNREHDIFDSDDYLQEHGG
                     MVATVRALRGGKAPQAFFGDSADPERPRVRTLAEEARRVVRSRVLNPRWISAMMRHGY
                     KGAFEMAATVDYLYGYDATARIGEDWMYEQVTERYVADP"
BASE COUNT          217 a          572 c          624 g          244 t
ORIGIN      
        1 tcggggtcgg cgacgtagcg ctcggtgacc tgttcgtaca tccagtcctc gccgatgcgg
       61 gccgtggcgt cgtagccgta gaggtagtcg acggtggccg ccatctcgaa ggcccccttg
      121 tagccgtggc gcatcatggc cgagatccag cgggggttga gcacccggga gcgcacgacc
      181 cggcgggcct cctcggccag ggtccggacc cggggccgct cggggtcggc ggagtcgccg
      241 aagaaggcct gcggcgcctt cccgccccgc agggcccgga cggtggccac catcccgccg
      301 tgttcctgga ggtagtcgtc ggagtcgaag atgtcgtgct cccggttgtc ctggttcttc
      361 accgccacgt cgatcagggc gaagcggcgc tgcatggcct ccggggccgg gacgccgtaa
      421 ccctggcggc cgtaggcata gccgctccag gccaggtaca cggtggccag gtcgtcgtcg
      481 ctctcccagt ccttggaatc gaggagggcc aggatgccgg agccgtaggc gccgggcttg
      541 gcgccgaaga tgcggtggtc gtcgccgtgg gcccggacga agttctgctc cggtggctcg
      601 tcgaggtggc gcacgagttc gacggcctcg tcgacgagat gcacgagatg ggggaaggcg
      661 tcccggaaga acccgctgat ccgcaacgtg acgtcgatgc ggggtcggcc gagttcttcg
      721 agggggacgg gctcgacgcc ggtgacccgg cccgactcct cggcccagac gggacgagcc
      781 ccgaggagag caagagcttc gccaatgtcg tcgccatggg tccgcatggc cgcggtgccc
      841 cacaccacga ggccgacgga cgccggccat ttcccggtct cctgccggtg gcgggcacag
      901 acggcatcgg ccagggcccg accgacgtcc cacgccgctt tcgtgggcag ggtcttcggg
      961 tcgacggagt agaagttccg ccccgtcggg agcacatggg ccatgccccg ggtcggtgcc
     1021 cccgacggcc ccgccggcac gtaccgccca tcgagggccc gcaacgtgtt ggtcacctcg
     1081 tcggtcgtgg cggcgaggtt cgggacgagc acgtcccctg cgaaccgcag cgcccgcccg
     1141 acctcgccca ccccgccgtc cccaccgtgg cggccgccga ctggcgttcc cgagcgcggt
     1201 cctgccgcac tgatgaacgc cggttcggtc gcttcggcat ccggcgttcg atttccgctg
     1261 gattccggta acggaacgcc agttgccgct gccccggccg cgcccggctt ctggcgctcg
     1321 atttccgcag tatcccggca aaggaacgcc ggttcggcgg gccaggcggc gtcgcggccg
     1381 gcgcgcagga ggcgcttggc ttcggcgtcg atggtgtcga cggcgtcaga cgccgtggtc
     1441 caggcgtggt cgccggtctc ggcgggatcg aggcggcggc ccggttcggc caggagctcc
     1501 ctgaggtcga ggcccagacc gtgcgccacg gccgagcgca gggaggggac cgagccctgg
     1561 gggaggcgca acagggcggc caggaggtcg atctcggcat cgccctccgg ggggcggccg
     1621 aaggtgtgga ggccgccgcg gatctgggcg tccttca
//
LOCUS       DASWGG010000305         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1889180, whole genome shotgun sequence.
ACCESSION   DASWGG010000305 DASWGG010000000
VERSION     DASWGG010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889180"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..897
                     /locus_tag="VF954_05460"
     CDS             214..897
                     /locus_tag="VF954_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX9530564.1"
                     /translation="MIEPVTPTTGWGVLHLFCRAGPGVEAEAVVAAVKATEAADHQVV
                     CVALLGHKAELGFMALGPDLWVLRRFQAALQGAGLEMAGSYLSLTEVSEYAQAMPEHA
                     KRARLYPRLPPDGKPAFCFYPMSKRRDSAHNWYALPFAERKELMAGHGEVGRTFAGRI
                     VQLITGSAGLDDWEWGVTLFGARVDDLKDVVYTMRFDPASAHFAEFGPFVTGLVGDVE
                     EILAELGVH"
     gene            972..1799
                     /gene="larE"
                     /locus_tag="VF954_05465"
     CDS             972..1799
                     /gene="larE"
                     /locus_tag="VF954_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX9530565.1"
                     /translation="MPADQLSLRDALAALRDELRRLERVVVAFSGGADSAFLAWVAHD
                     TLGPDRVLAVTAVSPSLASEELRECEQLAAEWGLRWQPVRTGELHDERYVTNAGDRCY
                     WCKTSLMEMAGPVAARERATVVLGVNLDDLGEHRPGQQAAAERGAAFPLVAAGFTKDD
                     VRAASRLLGLRTWDKPAAACLASRVPYGTKVTLGVLTSVAESESGLRGLGFRQVRVRH
                     YGDLARVEVPVADLARVTEHADEVVAAVRAAGYTYVTLDLEGFRSGNLNAALDRESP"
     gene            1796..2035
                     /locus_tag="VF954_05470"
     CDS             1796..2035
                     /locus_tag="VF954_05470"
                     /inference="COORDINATES: protein motif:HMM:NF020940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HEX9530566.1"
                     /translation="MTNVRVKLTFPEHLIKQPIIARMVREFDVMPNIRRANVEETVGW
                     IVCELGGDSGSIERAIEWLRDLGVAVNRLGDVVES"
     gene            2056..2559
                     /locus_tag="VF954_05475"
     CDS             2056..2559
                     /locus_tag="VF954_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530567.1"
                     /translation="MTGPAAEGDTALAQISASLADAIEASLAGWVTGCVERVVSAWVG
                     AVPETVRAEAEIAGQAAAAEVGPVVRTLLLRDIDDQSGTPLAILRRAVAYPTAVLLGA
                     GVPPVRRDDYSLERFPADIYDLTPANLADVDPALAELGLAWGAAKAWSHRRRHAGPAG
                     GPSGSGG"
BASE COUNT          363 a          956 c          959 g          377 t
ORIGIN      
        1 accaagtcca ctgccaccaa gtccactgcc accaagtcca ctgccaccaa gagggcgccc
       61 ggcgccaccg gcgcggccaa ggacaggtcg accacgaccg cgaccgccaa gcgcggccgc
      121 gccgcgccgc gctcccagga gacgtaggcc tcctccggcc ctcccggcgg tgcacgacct
      181 gcggttgccc cggcgcccct agaatcggcg ccgatgatcg agcccgtgac cccgaccacc
      241 ggctggggag tcctgcacct gttctgcaga gcgggccccg gcgtggaggc cgaggccgtg
      301 gtcgccgcgg tcaaggccac cgaggccgcg gaccaccagg tggtctgtgt ggccctgctc
      361 ggccacaagg cggaattggg gttcatggcc ctgggccccg atctctgggt cctgcgccgc
      421 ttccaggccg ccctccaggg cgccgggctg gagatggcgg gctcgtacct gtcgctcacc
      481 gaggtctccg agtatgccca ggcgatgccc gagcacgcca agcgagccag gctgtacccc
      541 cgcctcccgc cggacggaaa gccggccttc tgcttctacc ccatgtccaa gcgccgggac
      601 tcggcgcaca actggtacgc cctgccgttc gcggagcgca aggagctcat ggcgggccac
      661 ggcgaggtcg gccgcacctt cgcggggcgc atcgtgcagc tgatcaccgg ctcggccggg
      721 ctggacgact gggaatgggg cgtcacgctc ttcggggccc gcgtcgatga cctcaaggac
      781 gtggtctaca cgatgcggtt cgacccggct tcagcccact tcgcagagtt cgggcccttc
      841 gtcaccgggc tggtgggcga cgtcgaggag atcctggccg agctcggcgt gcactgacag
      901 cgtcggccag tgacaaatgc ggatggctgc cgcatgggcg agcggtcggg ccgacgagcc
      961 agaatggacg ggtgccggcc gaccagctct cccttcggga cgccctcgcc gccctccgcg
     1021 acgagctgcg ccgcctcgag cgggtcgtgg tggccttcag cggcggagcc gactccgcct
     1081 tcctggcctg ggtggctcac gacaccctgg gccccgaccg ggtgctggcc gtcaccgcgg
     1141 tgtcgccctc cctcgcgtcc gaggagctgc gggaatgcga gcagctggcc gcagagtggg
     1201 gcctgcgctg gcagccggtg cggacgggcg agctccacga cgagcgctac gtcaccaacg
     1261 ccggtgaccg gtgctactgg tgcaagacgt cgctcatgga gatggcagga ccggtcgccg
     1321 cacgcgagcg tgcgaccgtg gtgttggggg tgaacctcga cgatctcggg gagcaccgcc
     1381 ccggccagca ggccgcggcc gaacggggag cggcgttccc cctcgtcgcg gcggggttca
     1441 ccaaggacga cgtccgggcc gcatcccggc tgctgggcct gcgaacttgg gacaagccgg
     1501 cggcggcctg cctggcgtcg cgggtcccgt acgggaccaa ggtcaccctg ggcgtgctca
     1561 cgtcggttgc cgagtcggag tccggcctgc gcggcctggg tttccggcag gtccgggtca
     1621 ggcactacgg cgatctggcg cgcgtcgagg tacccgtcgc cgaccttgcc cgggtcaccg
     1681 agcacgccga cgaggtggtg gcggccgtcc gggcggctgg ctacacctac gtcaccctcg
     1741 atctggaagg cttccggtcg ggcaacctca acgccgccct cgacagggag tccccgtgac
     1801 caatgtgcga gtcaagctga ccttccccga gcacctcatc aaacagccga tcatcgcccg
     1861 catggtccgg gagttcgacg tcatgcccaa catccggcgg gccaacgtgg aggagacggt
     1921 tggatggatc gtgtgcgagc tgggcggcga ttcaggctcc atcgagcgcg ccatcgagtg
     1981 gctgcgcgac ctcggcgtcg ctgtcaaccg gctcggagat gtcgtcgaaa gctgacccgc
     2041 agcccggatg ccgccgtgac cggaccggcc gctgagggcg acaccgccct cgcccagatc
     2101 tcggcctctc tggccgacgc catcgaggcc agcctggccg ggtgggtgac cggttgcgtg
     2161 gagcgggtcg tgtcggcctg ggtgggcgcg gtgcccgaaa cggtgcgggc tgaggccgag
     2221 atcgccggcc aggcggccgc ggccgaggtc ggcccggtgg tgagaaccct tctgctgcgc
     2281 gacatcgacg accagtcggg cacgccgctg gccatcctgc gtcgagcggt ggcctatccg
     2341 acggcggtgc tcctcggcgc aggtgtgcct ccggtccggc gcgacgacta ctccctcgag
     2401 cggtttcccg cggacatcta cgacctcacg ccggccaacc tcgccgacgt cgacccggcc
     2461 ctcgccgagc tgggcctggc gtggggtgcg gccaaggctt ggagccaccg gcgccggcac
     2521 gcgggccccg ccgggggccc gtcggggagc ggcgggtgag ccgtatcctg gcctacgtgc
     2581 ccgatctgat ggaccggtcg cgtctcgcgg ggatccaggg cgtcgagctc gtcgcccggg
     2641 cgaccgacct cgcca
//
LOCUS       DASWGG010000306         3721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3176056, whole genome shotgun sequence.
ACCESSION   DASWGG010000306 DASWGG010000000
VERSION     DASWGG010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3176056"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1948)
                     /gene="typA"
                     /locus_tag="VF954_05480"
     CDS             complement(83..1948)
                     /gene="typA"
                     /locus_tag="VF954_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX9530568.1"
                     /translation="MPTRHDLRNVAIVAHVDHGKTTLVDAMLWQSGAFRANQDVAERV
                     MDSLDLEREKGITILAKNTTVRYRGVTINIVDTPGHADFGGEVERGLAMVDGVLLLVD
                     ASEGPLPQTRFVLRKALEQRLPVILVVNKVDRPDARIDEVVDEVYELFLDLDADEGQI
                     EFPIVYCNARAGWARLERPENGAAAPPTTEAAGLAPLMDLVLARIPAPIYEEGHPFQA
                     LVTNLDASPYVGRLALCRVHQGRVRKGQQVAWCRLDGTSERVKITELYLTEALDRVDA
                     AEAGPGDIIAVAGLAEVTIGETLADPDDPRPLPALTVDEPSLSLTIGVNTSPAAGQEG
                     AKLTARLIKSRLDAELVGNVSLRVLPTDRPDTWEVQGRGELQLAVLVEMMRREGFELT
                     VGKPQVLTRIVDGKLAEPMERMTVDVPEDFLGVVTQLLALRKGRMEHMVNHGTGWARL
                     EYLVPARALIGFRTEFLTETRGTGLLHHVFEAYEPWMGELRTRPTGSLVADRRGPTTG
                     YALSSLQERGVLFVGPGTEVYEGMIVGENARADDMDVNPTKEKKLTNHRASGAEVLER
                     LVPYQQLSLEQALEFCRDDECAEVTPASVRLRKVVLSQQDRVRQFRRQVRQTVQG"
     gene            2213..3187
                     /locus_tag="VF954_05485"
     CDS             2213..3187
                     /locus_tag="VF954_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX9530569.1"
                     /translation="MDRVERLTNLVLVLLHTRVPMTLQEVADALEGAYPDRREARRQA
                     FERDKRLLREEGIDVSVLPVGPDGSGQPQFGYLIRPEDYYLPDLELDADEQIALNLAV
                     AAVRLDEGSGRDVLWKLGGLARDAPPPLAALPALPQLPVLHEAIRSRRRAAFCYRGRP
                     DARHLEPYGMVFRGGFWYVVGRDRDRGEQRVFRVDRIEGDVEVGAPAAFERPEGFDAV
                     AAVRGDPWRFGEGDERRALVLVDRVQAPKVEAELGSAAVVERRDGGEIVVTLQVTSVA
                     GFRPWLLGLVDHAVVIGPPPLRAEVVSWLEAMAAAPAGGPAAGSLEPA"
     gene            3184..>3721
                     /locus_tag="VF954_05490"
     CDS             3184..>3721
                     /locus_tag="VF954_05490"
                     /inference="COORDINATES: protein motif:HMM:NF039255.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530570.1"
                     /translation="MTPPAPPRPTSADRLRRLLAMLPWLASAGGASVRDLAERFGLPD
                     DEVVAELEMAACCGLPPYSPDQLIELIIDGDRVEANFGGLLARPQRFTPAEGFTLAAS
                     ARALLAVPGADPTGALARAVAKLDAVLGHRAVAVDLDEPPLLSEVRAAIERRESAEIE
                     YYAATRDELTTRRVDPYVV"
BASE COUNT          455 a         1324 c         1397 g          545 t
ORIGIN      
        1 cgacgaacgg cgacaccgtg ggacatttct cccgggcttc ccgcgaaccg ccggatccgc
       61 ccgacccgcc cgaccgctcg gctcagccct ggacggtctg gcggacctgg cggcggaact
      121 gccggacccg atcctgctga gacaggacga ccttgcgcag ccgtaccgac gccggggtga
      181 cctcggcgca ctcgtcgtcc cggcagaact ccagcgcctg ctcgagggag agctgctggt
      241 agggcaccag ccgctccagg acctcggcgc cggaggcccg gtggttggtc agcttcttct
      301 ccttggtggg gttgacgtcc atgtcgtcgg cccgggcgtt ctcaccgacg atcatcccct
      361 cgtacacctc ggtgccgggc cccacgaaga gcacccctcg ttcctgcagg ctggacaggg
      421 cgtagccggt cgtcgggccc cgccggtcgg ccaccaggct gccggtggga cgggtccgca
      481 gctcacccat ccacggctcg taggcctcga acacgtgatg gagcaggccg gtgccgcggg
      541 tctcggtcag gaactccgtc cggaacccga tcaacgcccg cgccggcacc aggtactcga
      601 gccgggccca gcccgtgccg tggttgacca tgtgctccat ccgccccttc cggagggcga
      661 gcagctgggt gacgaccccg aggaagtcct cgggcacgtc gacggtcatg cgctccatcg
      721 gctcggcgag cttgccgtcc acgatcctgg tcaatacctg gggcttgccg acggtgagct
      781 cgaagccctc ccggcgcatc atctcgacca gcacggcgag ctggagctcg ccccggccct
      841 ggacctccca ggtgtcgggc cggtcggtcg gcagcacgcg cagcgacacg ttgccgacca
      901 gctcggcgtc gaggcggctc ttgatgagcc gggccgtcag cttggcgccc tcctggccgg
      961 cggcggggct ggtgttgacc ccgatcgtca gcgacaggct cggctcgtcg acggtgaggg
     1021 ccggaagcgg acgggggtcg tccgggtcgg ccagcgtctc cccgatggtg acctcggcca
     1081 ggccggcgac ggcgatgatg tcgccgggcc cggcctcggc ggcgtccacg cggtccaggg
     1141 cctcggtgag gtacagctcg gtgatcttca cccgctccga cgtgccgtcc aggcggcacc
     1201 aggccacctg ctggcccttg cggacgcggc cttgatggac gcggcacagg gccagccggc
     1261 cgacgtaggg cgaggcgtcg aggttggtga ccagggcctg gaacgggtgg ccctcctcgt
     1321 agatcggggc cgggatccgc gccaggacca ggtccatgag cggcgccagg ccggcggcct
     1381 cggtcgttgg cggggcggcg gcgccgttct ccggtcgctc gaggcgcgcc cagccggccc
     1441 gggcgttgca gtagacgatc gggaactcga tctggccttc gtccgcgtcc aggtcgagga
     1501 acagctcgta gacctcgtcc accacctcgt cgatgcgtgc gtcgggccgg tccaccttgt
     1561 tgaccacgag gatgacgggc aggcgttgct ccaaggcctt gcgcagcacg aagcgggtct
     1621 gcgggagcgg gccctcgctg gcgtcgacga gcagcaggac gccgtccacc atggccagcc
     1681 cgcgctcgac ctcgccgccg aagtcggcgt ggccgggcgt gtcgacgatg ttgatggtga
     1741 cgccccggta ccggacagtc gtgttcttgg ccaggatggt gatgcccttc tcccgctcga
     1801 gatcgagcga gtccatcacc cgctcggcga cgtcctgatt ggcccggaac gcgccggact
     1861 gccacagcat ggcgtcgacg agcgtcgtct tgccgtggtc gacgtgggcg acgatggcca
     1921 cgttgcggag gtcgtggcgc gtgggcatgg gttctccgag tcgggcgggg gaagtccggg
     1981 gcgggccgcc accggtcgtg cgaggccgcg ggcaggagtc acgcccgccg agcagccatg
     2041 gtacccgcta ggcgggctcg cccggcccct tgtgatcagc cgggccgggc aggaccggcc
     2101 ggtcccacgg gtggcctggt cgagatccgg ccagcccgtg aggtcgagcg cggcgtgtgg
     2161 ccgcgccgcc cgccagcgcc ggtacgcctc tggatcgtta ctctggccgg ccatggatcg
     2221 ggtcgagcgc ttgacgaacc tggtcctggt gttgctccac acccgcgtgc ccatgacgtt
     2281 gcaggaggtt gccgatgccc tggagggcgc ctaccccgat aggcgcgagg cccgccggca
     2341 ggccttcgag cgggacaagc gtctcctgcg ggaggagggc atcgatgtca gcgtcctgcc
     2401 cgtgggcccg gatgggtccg gccagcccca gttcggctac ctgatccggc cggaggacta
     2461 ctacctgccg gacctggagc tcgacgccga cgagcagatc gccctaaacc tcgccgtggc
     2521 cgcggtgcgc ctcgacgagg gcagcgggcg tgacgtcctg tggaagctcg gtgggctggc
     2581 gcgagacgcg ccgccgccgc tcgccgccct gcccgccctg ccccagctcc cggtgctgca
     2641 cgaggccatc cgctcccgcc gccgggcggc cttctgctac cggggccggc cggatgcccg
     2701 ccatctggag ccgtacggga tggtcttccg cggcgggttc tggtacgtgg tgggccgcga
     2761 ccgggaccgg ggcgagcagc gggtcttccg tgtcgaccgc atcgagggcg acgtggaggt
     2821 gggcgcgccc gccgcgttcg agcggccgga gggcttcgac gcggtcgcgg ccgtgcgggg
     2881 cgatccctgg cgtttcgggg agggcgacga acggcgggcg ctcgtcctcg tcgaccgggt
     2941 ccaggccccg aaggtggagg ccgagctcgg ctcggcggcg gtcgtcgaac ggcgggacgg
     3001 gggcgagatc gtggtcaccc ttcaggtgac gagcgtggcc gggttccggc cgtggctgct
     3061 ggggctggtc gaccacgcag tggtgatcgg gcccccgccg ctgcgggccg aggtggtgag
     3121 ctggctcgag gccatggctg cggcccctgc gggcgggccg gccgccggga gcctggagcc
     3181 ggcgtgaccc cgccggcgcc gccgcgcccc acgtcggccg accggctgcg acgcctgctg
     3241 gccatgctgc cgtggctggc cagcgcgggt ggggcgtcgg tgcgcgacct ggccgaacgc
     3301 ttcgggctgc ccgacgacga ggtcgtggcc gagctcgaga tggccgcctg ctgcgggctg
     3361 cctccctact cgcccgacca gctcatcgag ctgatcatcg acggtgatcg ggtggaagcc
     3421 aacttcggtg gcctgctggc ccggccccag cgcttcacgc cggccgaggg gttcaccctg
     3481 gcggcctcgg cccgggccct cctcgccgtc cctggagccg acccgaccgg cgccctggcc
     3541 cgggcggtcg ccaagctgga cgccgtgctg ggccaccggg cggtggcggt ggacctggac
     3601 gagccgcccc tgctcagcga ggtgcgggcc gccatcgaac gccgcgagtc cgccgagatc
     3661 gagtactacg cggcgactcg cgacgagctg accacccggc gggtggatcc ctacgtcgtc
     3721 t
//
LOCUS       DASWGG010000307         5079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3176126, whole genome shotgun sequence.
ACCESSION   DASWGG010000307 DASWGG010000000
VERSION     DASWGG010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3176126"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..909)
                     /gene="whiG"
                     /locus_tag="VF954_05495"
     CDS             complement(130..909)
                     /gene="whiG"
                     /locus_tag="VF954_05495"
                     /inference="COORDINATES: protein motif:HMM:NF004935.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HEX9530571.1"
                     /translation="MDTDDVKAIDHLWAEYKATGSRDARDRLIVHYSPLVKYVAGRVS
                     VGLPQNIEQADLVSYGIFGLIDAIDKFDPARNIKFETYAIARIKGAIIDELRSIDWVP
                     RSVRSKARNVEKAYAKLEANLLRTPTDLEVASEMGISENDLHNVFNQISFVGLVALDE
                     MLSGGDRGESTTLGDTIADKGEGPVALYEVAEMKQILASAINRLADREKIVLTLYYYE
                     GLTLAEIGQVLGVTESRVCQIHTKAVLQLRSKMTEPEREPA"
     gene            complement(918..1748)
                     /locus_tag="VF954_05500"
                     /pseudo
     CDS             complement(918..1748)
                     /locus_tag="VF954_05500"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
     gene            complement(1881..3155)
                     /locus_tag="VF954_05505"
     CDS             complement(1881..3155)
                     /locus_tag="VF954_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEX9530572.1"
                     /translation="MTAGPASRQPLPEEAFAVALARLPAMGWRRMGALIAGRSGQEAW
                     EVVKAGRVLEVVPPIGPASPSRVLDRASDSWRARACGLDVAASWSRTCALGITVRARG
                     RAGYPEVLDEDPEPPAVVFHLGAVPSPGHRRVAVVGTRHCSYEGREAARSIGYGLAEA
                     GVTVVSGLALGIDGAAHQGALAAGADGEGVGPPFGVVASGLDVPYPARHAGLWRQVAV
                     AGGLLSEYPPGSPATRMRFPARNRLIAAFAEIVVVVESRVAGGSRHTVEAAIARGRTV
                     MAVPGSIRNPAAAGTNRLIAEGCAPVCDVADILVALDLERAGRPEPAPRPDRRAGAAA
                     QPGRRPVSTSGPAGITGEGVGAPSPARRTRPDLSRAEQLVLDAVGGTPASLDTLLLRT
                     GAGPLALTAALERLRAAELVVEDAGWWSLRPR"
     gene            complement(3152..4654)
                     /locus_tag="VF954_05510"
     CDS             complement(3152..4654)
                     /locus_tag="VF954_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX9530573.1"
                     /translation="MLASIPAASLVGVEGRSVLVEVHVSTGLPTFTVVGLPDAACRES
                     RDRVRAAVLSSGLRWPQQRITVNLAPSGVRKGGAALDLAIAIGVLVASDVLAPDLVSD
                     CAFVGELGLDGSIRRVPGILSLTGAIRAGAVVVAASAAPEAGLAGGPVVRPVTSLRHL
                     LSALRGLEGWSDLGPSPAMPAVALPDLADVRGQPTGRWALEVAAAGGHHLLMMGPPGA
                     GKTMLAERLPSLLPALGRDEAIEVLRIQSVAGASPSGHLPRWPPFRAPHHSASAVAVV
                     GGGSSVLRPGEISLAHRGVLFLDELGEFPAVVLDQLRQPLEEGVIRVARAAGSASFPA
                     RFLLVAATNPCPCGQDGGPLSCQCPPAARLRYLRRLSGPLLDRFDLRVPVFRPDAAQL
                     LGAPPGEASAVVAARVARARERARARGWPCNAAIRAGELDRWAPLSAAGSRLLGRRLT
                     AGGLSARGLDRIRRVGLTLADLEGDDVPLTEAHLSAALALRSSLAVQQAA"
     gene            complement(4765..>5079)
                     /locus_tag="VF954_05515"
     CDS             complement(4765..>5079)
                     /locus_tag="VF954_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9530574.1"
                     /translation="RAKELAFLAEIISAKEAAEIGVVNRVVPAADLDAFVDDWARRLA
                     QGPPLALSLTKAMLNNSLGLSMDEALEAEAHAQAIALTSVDGREAIAAFRDKREPHFQ
                     GR"
BASE COUNT          698 a         1883 c         1914 g          584 t
ORIGIN      
        1 ccggcaagtg accccaccag cagcgcgagc ccggcgaggc gggcgggcgg ccgcatccgg
       61 ggatggtagg ccgcacggcc ccgccgggct caagttggcg gccggccggc gcggcgacgg
      121 gcaaccccgt caggccggct cgcgctccgg ttcggtcatc ttggaccgca gctggagcac
      181 ggccttggtg tggatctggc agacccggct ctcggtgacg ccgaggacct ggccgatctc
      241 ggccagggtc aggccctcgt agtagtagag ggtcaaaacg atcttctccc ggtcggccag
      301 ccggttgatg gccgaagcca ggatctgctt catctcggcg acctcgtaga gcgccaccgg
      361 gccctcacct ttgtccgcga tggtgtcgcc caaggtggtc gactcaccac ggtcgccccc
      421 cgacagcatc tcgtcgagcg ccaccaggcc gacgaaggag atctggttga acacattgtg
      481 caggtcgttc tcgctgatcc ccatctcgga cgccacctcg aggtcggtcg gggtccgcag
      541 gaggttggcc tccagcttgg cgtaggcctt ctccacgttg cgtgccttgg accgcaccga
      601 gcggggcacc cagtcgatcg agcgcagctc gtcgatgatg gcccccttga tccgggcgat
      661 ggcgtaggtc tcgaacttga tgttccgggc cgggtcgaac ttgtcgatgg cgtcgatcaa
      721 gccgaagatc ccgtagctga ccaggtcggc ctgctcgatg ttctggggca gacccaccga
      781 cacccggccg gccacgtact tgacgagcgg cgagtagtgg acgatcagcc ggtcccgagc
      841 gtcccgggag ccggtcgcct tgtactcggc ccacagatga tcgatggcct tcacgtcgtc
      901 ggtgtccaag tcgagtccta ggctcggggg tgcgtgcggt cgtagacctg caggaggcgt
      961 tccttgctta catgagtgta gacctgcgtc gtggacaggc tggcgtgccc tagcagctcc
     1021 tgcacgacgc gcaggtcggc gcccccatcg aggaggtggg tggcgaaggt gtggcgcaga
     1081 gcgtgagggt gggtcggccg gggagcgcgg tgatcgagga tgcgctgcac gtcccgccgg
     1141 ccgaggcggt ggccccgccg gttcaggaag acggcgctcg gaggggagtc ggaccgtgcc
     1201 aggcgcgacc gcccccgctc cagccaaccg tccagggcgt cggcggccgg ccggctgacc
     1261 gggacctggc gggccttcga gcctttgccg acgaccgtga ccacgccccg tggccggtcg
     1321 atcccgtcca gcgtcagctg gcacagctcg gacacccgga gcccgctgcc gtagagaagc
     1381 tcgagcacgg cgtcgtcccg gagccggacc gcggcgtcgt cgtcccccac cggagcgggc
     1441 ggctcgtcca gcagcacgac gagctcggcc ccgctcagga cccgcgggag gcgcccctcc
     1501 ccggtggggg ccgtcaggcg gagggcgggg tcggccggga tggccccggt gcgggccagc
     1561 cacgcgaagt agcgccgcac cgccgacgcc ttgcggcgga tgctgcgccg ggcgtacccg
     1621 caggtcgcca ggtaggccag ccaccggcgc agcacgagcc gggtcaccga gtccggttgg
     1681 cggtggccac cgcgctcggc ccactccacg aagccctcca cgtcgcgcga gtaggccgtc
     1741 acggttgccg gcgcagccga cgtcagcgat cgggcgaagc cctcgacctg ccatcccacc
     1801 aacagaaggg tacccaccgc gctgggcgcg aacgggcatc ggggtcccgt cggccgtgga
     1861 tcgacccgcc atctgcacct tcatctgggc cggaggctcc accagccggc gtcctcgacc
     1921 accagctcgg cggcccggag ccgctccagc gcggcggtga gggcgagggg gccggcaccg
     1981 gtccgcagca acagggtgtc cagggaggcc ggcgtcccgc cgacggcgtc cagcaccagc
     2041 tgctcggcac gcgacaggtc cggccgggtc ctccgagccg gcgagggcgc cccgacgccc
     2101 tcgcccgtga tcccggccgg gcccgacgtc gacacagggc gacggcccgg ctgagcggcc
     2161 gcgccggccc gacggtccgg gcgcggggcc ggctccggcc ggcccgcccg ttcgaggtcg
     2221 agggcgacca ggatgtcggc cacgtcgcac accggagcgc agccctcggc gatgaggcgg
     2281 ttggtgccgg ccgccgccgg gttgcggatc gagccgggca cggccatcac cgtgcggccc
     2341 cgggcgatgg ccgcctccac ggtgtggcgg gacccgccgg ccaccctgga ctctacgacc
     2401 accacgatct cggcgaaggc ggcgatcaag cggttgcggg ccgggaaccg catccgggtg
     2461 gcgggagagc cgggcgggta ctccgacagg agcccgccgg cgactgccac ctggcgccac
     2521 aagccggcat ggcgagccgg gtagggaacg tcgaggccgc tcgccaccac gccgaacggg
     2581 ggcccgaccc cttccccgtc cgccccggcg gccagggcgc cctggtgggc ggcgccgtcg
     2641 atcccgagag ccaggccgga cacgacggtc acgcccgcct cggccaggcc gtagccgatc
     2701 gagcgggccg cctcacgccc ttcataggag cagtgccggg tccccaccac cgcgacacgc
     2761 cggtgcccgg gcgacgggac ggccccgagg tggaagacca cggcgggcgg ctccgggtcc
     2821 tcgtccagca cctcggggta gcccgcccgg ccgcgggccc gcacggtgat gccgagcgcg
     2881 cacgtccggc tccacgacgc ggccacatcg aggccgcaag cccgggcccg ccagctgtcc
     2941 gatgcccggt cgaggaccct gctgggactg gccggcccga tgggtggcac gacctcgagc
     3001 acccgaccgg ccttcaccac ctcccacgcc tcctggccag agcgcccggc gatcagggca
     3061 cccatccggc gccagcccat cgccggcagg cgagccaggg ccacggcgaa ggcctcctcc
     3121 ggtagcggct ggcgcgatgc cgggccggcc gtcacgccgc ctgctggact gccagagacg
     3181 agcgcagcgc gagggccgcg ctcaggtggg cctcggtcag gggcacgtcg tcgccctcca
     3241 ggtcggcgag cgtgagcccg acccgccgga tgcggtcgag gccccgggcg ctgagcccgc
     3301 ccgccgtgag ccgccggccg agcagccggg accccgccgc cgacagcggc gcccaccggt
     3361 ccagctcccc tgcccggatg gcggcgttgc acggccagcc ccgggcgcgc gcccgctccc
     3421 gggcccgggc gacccgtgcg gccaccacgg cggacgcctc gcctggcggg gcacccagca
     3481 gctgggcggc gtcgggacgg aacaccggca cccggagatc gaaccggtcg agcagcggcc
     3541 ccgacagccg gcggaggtag cggagccggg cagccggcgg gcactggcac gacagcggac
     3601 cgccgtcctg cccgcacggg cagggattgg tcgccgccac cagcaggaac cgggccggga
     3661 agctggccga gccggcggcc ctggcgaccc ggatcacccc ctcctcgagc ggttggcgaa
     3721 gctggtcgag cacgaccgcc gggaactcgc ccagctcgtc gaggaacagg accccgcggt
     3781 gggcgaggct gatctccccg gggcgcagga cggaggagcc gccgccgacc acggccacgg
     3841 cggaggcgct gtggtgcggg gcccggaacg ggggccaccg cggcaggtgg cccgatgggc
     3901 tcgcacccgc cacggactgg atgcggagca cctcgatcgc ctcgtcgcgg cccagagccg
     3961 gcagcagcga cgggaggcgc tcggcgagca tggtcttgcc ggcacccggc gggcccatca
     4021 tcagcaggtg gtggccgccg gcggcggcca cctccaacgc ccagcgcccg gtcggctggc
     4081 cccggacgtc ggccaggtcg ggcagcgcga cggccggcat cgccgggctc ggccccaggt
     4141 cggaccagcc ctccaggcca cgcagcgcac tcagcaggtg gcggaggctg gtcacgggcc
     4201 ggaccaccgg gcccccggcc aggccggcct cgggggcggc ggacgccgcg accacgaccg
     4261 ccccggcccg gatggcgccg gtcagcgaca ggatgcccgg cacccggcgg atgctcccat
     4321 ccagccccag ctcgccgacg aaggcgcagt cggacaccag atccggcgcc agcacgtcgg
     4381 aggccaccag cacgccgatg gcgatggcca ggtcgagggc cgcgccgccc ttgcgcacgc
     4441 ccgacggggc caggttgacg gtgatgcgct gctggggcca gcgcaacccg gaggacagga
     4501 cggcggcccg cacccggtca cgcgactccc ggcaggcggc gtcgggcagg cccacgaccg
     4561 tgaaggtggg caatccggtg gacacgtgga cttctaccag gacggagcgt ccttcgacgc
     4621 cgaccaggct ggcggcgggg atgctggcga gcacggggat ctcctgcgtc ggggtgcggg
     4681 caggagggcc gtggcgccgg ccgctcgagg cgtgtccctc agtctagagc aggtctgtgt
     4741 caggcccccg ccggcccgtc ggggtcaccg gccctggaag tgcggctcgc gcttgtcccg
     4801 gaaggccgcg atggcctcgc gcccgtcgac gctggtcagg gcgatggcct gggcgtgggc
     4861 ttcggcctcg agagcctcgt ccatcgacag gccgaggctg ttgttgagca tggccttggt
     4921 gagggagagg gcgagcggcg ggccctgggc caggcgccgg gcccagtcgt cgacgaaggc
     4981 gtcgaggtcg gcggcgggca cgacccggtt gaccacgccg atctccgccg cttccttggc
     5041 ggagatgatc tcggcgagaa aggccagctc cttggcccg
//
LOCUS       DASWGG010000308         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3176895, whole genome shotgun sequence.
ACCESSION   DASWGG010000308 DASWGG010000000
VERSION     DASWGG010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3176895"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VF954_05520"
     CDS             <1..316
                     /locus_tag="VF954_05520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379272.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX9530575.1"
                     /translation="DRKVTPPPRARIDESMEALIHHFKVFTEGFKVPPGETYVAVENP
                     RGELGCYLVSDGSGRPYRMHIRGASFYNLQTLSTMVQGGLVADGIAIISSIDPIMGDV
                     DR"
     gene            313..780
                     /locus_tag="VF954_05525"
     CDS             313..780
                     /locus_tag="VF954_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX9530576.1"
                     /translation="MIPADEILARYPSKRSALMPLLYEVAERDGYVTEAGMEEVAEHI
                     GLTAAEVLGVCSFYSMFKRSPQGKLLVSVCTSVSCMVNGGEELLAALRTRYADDPDVT
                     VEEVECLAADDGAPVMQVNYEFHERLTPESAAGIIEDYKAGRLTPRGPSGVRR"
     gene            777..>1969
                     /gene="nuoF"
                     /locus_tag="VF954_05530"
     CDS             777..>1969
                     /gene="nuoF"
                     /locus_tag="VF954_05530"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX9530577.1"
                     /translation="MTATETRIVTKRLRERPDDSWTLAAVKATGGYDSLRKALAMEPS
                     AIAEEVKASGLRGRGGAGFPTATKWSFLPAGVEPRYLVVNSDEAEPSTFKDRMLVERD
                     PHQLVEGIAIASYALNVHHAFVYVRGEFALGAERLAQACREARADGLIGPDMCGSGFG
                     LEITVHRGAGAYICGEETALLNSLEGDRGMPRIRPPFPAIAGLYAKPTVVNNVETIST
                     LPAIIQMGGAAYGALGVERSRGSRIFSLSGTVNRPGNYELELGLTFRQLIDDFGGGLR
                     GPGPVKFFIPGGASSQWLTHEHLDVPLDMDLVTQQYGVMLGSGAVMIYNQDVCAVRVA
                     LRLAKFFAHESCGKCTPCREGTGWIEKVLYRIEHGEGRSTDLDLLLDVGDNISPGLNA
                     PFSQTT"
BASE COUNT          294 a          682 c          694 g          299 t
ORIGIN      
        1 ggaccggaag gtgacgcccc cgccccgagc ccgcatcgac gagtcgatgg aggctctcat
       61 ccaccacttc aaggtgttca ccgagggctt caaggtgccg ccgggggaga cctacgtggc
      121 cgtcgagaac ccccggggcg agctgggctg ctacctcgtg tcggacgggt cgggccgtcc
      181 ctaccggatg cacatccggg gcgcctcgtt ctacaacctg cagaccctgt cgacgatggt
      241 ccagggcggc ctcgtggccg acggcatcgc catcatctcc agcatcgacc cgatcatggg
      301 ggacgtggat cgatgatccc cgccgacgag atcctggcgc ggtatccgtc gaagcggtcg
      361 gcgttgatgc cgctcctgta cgaggtggcc gagcgcgacg gctacgtcac cgaggcgggc
      421 atggaggagg tggcggagca catcgggctc acggcggccg aggtgctcgg ggtctgctcc
      481 ttctactcca tgttcaagcg ctcgccgcag gggaagctgc tggtgtcggt gtgcacgtcg
      541 gtgtcgtgca tggtcaacgg cggcgaggaa ctgctcgccg ccctgcggac gcgttacgcc
      601 gatgatccgg acgtcacggt ggaagaggtg gagtgcctcg ccgccgacga cggggcgccg
      661 gtcatgcagg tcaattacga gttccacgag cggctgacac ccgagtcggc cgccgggatc
      721 attgaggact acaaggccgg ccggctcacg ccgcggggtc cgtcgggggt gcgccgatga
      781 cggccaccga gacgcggatc gtcacgaagc ggctgcggga gcggcccgac gactcgtgga
      841 ccctggccgc cgtgaaggcg accggcggct acgacagcct ccgcaaggcc ctggccatgg
      901 agccttcggc catcgccgag gaggtcaagg cgtcggggct gcggggccgg ggcggggcgg
      961 ggttcccgac cgccaccaag tggtcgttcc tccccgccgg cgtcgagccc cgctacctcg
     1021 tcgtcaacag cgacgaggcc gagccgtcga cgttcaagga ccggatgctc gtcgagcgcg
     1081 acccccacca gctggtggag ggcatcgcca tcgccagtta cgccctcaac gtgcatcacg
     1141 ccttcgtcta cgtccggggc gagttcgccc tcggggccga gcggctggct caggcctgtc
     1201 gcgaggcccg ggccgacggg ctcatcgggc ccgacatgtg cgggtcgggc ttcggcctcg
     1261 agatcaccgt ccaccggggc gccggggctt acatctgcgg cgaggagacg gcgctgctca
     1321 acagtctgga gggtgaccgg ggcatgcccc gcatccgccc gccgttcccg gccatcgccg
     1381 gtctctacgc caagccgacc gtggtcaaca acgtcgagac gatctcgacc ctgccggcca
     1441 tcatccagat gggcggcgcg gcctacgggg cgctcggggt cgagcgttcc cgggggagcc
     1501 gcatcttctc cctgtcgggc accgtgaacc ggcccggcaa ctacgagctg gagctcggtc
     1561 tcaccttccg gcagctcatc gacgacttcg gcggcggcct gcgggggccg ggcccggtca
     1621 agttcttcat ccccggcggc gcctcgtcgc agtggctgac ccacgagcac ctcgacgtcc
     1681 ccctcgacat ggacctcgtc acccagcagt acggcgtgat gctggggtcg ggggcggtca
     1741 tgatctacaa ccaggacgtc tgcgccgtgc gggtggcgct gcggctggcc aagttcttcg
     1801 cccacgagag ctgcggcaag tgcaccccct gccgggaggg gacgggctgg atcgaaaagg
     1861 tgctgtaccg gatcgagcac ggcgagggtc ggtcgacgga cctcgacctc ctcctcgacg
     1921 tcggcgacaa catctcgccc ggcctcaacg cccccttttc ccagaccac
//
LOCUS       DASWGG010000309         4535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_921147, whole genome shotgun sequence.
ACCESSION   DASWGG010000309 DASWGG010000000
VERSION     DASWGG010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921147"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="VF954_05535"
     CDS             complement(<1..1389)
                     /locus_tag="VF954_05535"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HEX9530578.1"
                     /translation="MRRPSGAALAAAIVILGVAGITLWQLHPSLVFLDTTPAGGDTGA
                     HVLVPAFLRDHLLPHGRLTGWSPSWYDGFPALTFYFPLPSLIIVAFNLVLPYGVAFKL
                     VTVLGLLTLPLAAYAFGRMMDMRDPGPACLAAATLPFLFDRTFTIYGGNVASTLAGEF
                     AFSISLSLALVFLGVVAKGQRSGRHRALAAVLLAVTALCHVIPTFFALAGWAVLTLMR
                     PARRTLWWTASTGVVGGALVGFWAVPFLARLAYTNDMGWEKITTYHKTLLPASLDWLV
                     ALALVGVVASVALRRRTGIFLSVMAVLSWAGFCLVPQSRLWNARLLPFWFLCLYLLAG
                     VAVAELGAAAVALWRGTWRELWDGGGGRWVYLESFHEHLEGGPIDDGWVTRTAAQVAA
                     ALRPLHDAGRGHGSVRPRTILIRSDGPVDVRAGVDGEPLLEVLGYASPEGIDGKAATP
                     TSDVWSLGAVVYE"
     gene            complement(1465..1605)
                     /locus_tag="VF954_05540"
     CDS             complement(1465..1605)
                     /locus_tag="VF954_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530579.1"
                     /translation="MEISLCVADKQVVMRSCSHCETRWWAGDGQRLGLGHVLELAAVG
                     RR"
     gene            complement(1732..2412)
                     /locus_tag="VF954_05545"
     CDS             complement(1732..2412)
                     /locus_tag="VF954_05545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX9530580.1"
                     /translation="MPAWGAGVAVGGFLVGTVLGSVAAALWVAVSGDRSGLNGYGAEI
                     AGLIGLWVGLVGSAVVVAAPRGGLGPALGLRFRPSDLPVGVAVGLLCQFPMVWALYLP
                     VRLLAPRTYRHLGDAATQMTRVAHGAGFVVLGALIAVGAPIAEEIFFRGLFQRSLGAL
                     YPSPVGVGAAAVFFGLAHFEALQLLALVAFGIVLGALAHRSGRLGPGIVAHMTFNAAT
                     VVVLAGLR"
     gene            complement(2430..2642)
                     /locus_tag="VF954_05550"
     CDS             complement(2430..2642)
                     /locus_tag="VF954_05550"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HEX9530581.1"
                     /translation="MRPGCGGSASATLTYDYARRTVWLDSMDEELEPGAWAMCSAHAD
                     GLRVPIGWARDDRRTPVIPLRPQIAV"
     gene            2869..>4535
                     /gene="ftsH"
                     /locus_tag="VF954_05555"
     CDS             2869..>4535
                     /gene="ftsH"
                     /locus_tag="VF954_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX9530582.1"
                     /translation="MIAVLVLVVLVLLFSGSLGGGKSKTSISYNDFITDVSHGQVDSA
                     TYNNDTGSISGTLKAGGRFSTGGPHPAIPADVQTMRENGVKLTFKNNSSNVLGSLLLY
                     LLPIVLLIGFFVYMNRRAQGQMSGIMSIGRSKAKVYTTERPRTTFNDVAGYTGVKQEI
                     REVVDFLKTPARFKDIGAKIPKGVLLVGPPGTGKTLLARAVAGEAGVPFMSVTGSDFM
                     EMFVGVGASRVRDLFQTARKQAPAIIFVDEIDSIGRKRGAGLGGGHDEREQTLNQMLA
                     EMDGFETTEGVVMMAATNRPDILDPALLRPGRFDRQIVVPLPDLEERLPILQVHSRDK
                     KVGPDVDLNLVARGTPGMSGADLANLVNEAALHAVRRGSDSIQMQDFEAARDRVIMGQ
                     RRETMALSMDEKERIAYHEGGHAVLAYVLPTADPVHKVTILPTGMALGVTQQLPMEER
                     HIYPRDYIEDSLAARMGGRVAEMLTYGDLSTGANNDLVGNTELARKMVREWGMSEAIG
                     PMAWGSQGQVFLGEDLMHTRDYSDETSCVIDEEVERILREQEDRATKA"
BASE COUNT          774 a         1618 c         1632 g          511 t
ORIGIN      
        1 ctcgtacacc accgcaccca gcgaccagac gtcggaggtg ggcgtggccg ccttaccgtc
       61 gatgccttcg ggggaggcgt agccgaggac ctccaggagg ggctcgccat ccaccccggc
      121 ccgcacgtcc accggcccgt ccgaccggat gaggatggtg cgcggccgga cgctgccgtg
      181 gccgcgaccg gcgtcgtgca gggggcgcag ggcggcggcg acctgggccg ccgtccgcgt
      241 gacccagccg tcgtcgatcg gaccgccctc gaggtgctcg tggaagctct ccaggtagac
      301 ccagcggccg ccgcccccgt cccacagctc ccgccacgtg ccccgccaca gggccaccgc
      361 ggcggcgccg agctcggcga cggcaacgcc cgccagcagg tacaggcaca ggaaccagaa
      421 cgggagcagc cgggcgttcc agagccggct ctggggcacg aggcagaacc cggcccagga
      481 gagcacggcc atgacggaaa ggaagatccc cgtgcgccgg cgcagggcga ccgacgccac
      541 cacgccgacc agggccagcg cgaccagcca gtcgagcgag gcgggcagga gggtcttgtg
      601 gtaggtggtg atcttctccc agcccatgtc gttggtgtag gccagccggg ccaggaaggg
      661 gacggcccag aacccgacga gggcgccgcc gaccacgccg gtcgaggcgg tccaccacag
      721 cgtccggcgc gccgggcgca tgagcgtgag caccgcccat ccggccaggg cgaagaaggt
      781 cgggatgacg tggcacaggg ccgtcacggc cagcaggacc gcggccagcg cccggtggcg
      841 cccgctgcgc tggcccttgg ccaccacgcc gaggaacacc agcgccagcg acaggctgat
      901 ggagaaggcg aactcccccg ccagcgtgga ggccacgttg ccgccgtaga tggtgaaggt
      961 gcgatcgaag aggaacggga gggtggccgc ggccaggcag gccgggcccg ggtcgcgcat
     1021 gtccatcatc cggccgaagg cgtaggcggc gaggggcagg gtcagcaggc ccaggacggt
     1081 caccagcttg aaggcgacgc cgtagggcag caccaggttg aaggcgacga tgatcaggct
     1141 gggcagcggg aagtagaacg tgagagccgg gaacccgtcg taccacgagg ggctccaccc
     1201 ggtgaggcgg ccgtgcggga ggaggtggtc ccgcaggaag gccggcacga ggacgtgggc
     1261 gcccgtgtcg ccgcccgccg gcgtggtgtc gaggaagacg agcgacgggt gcagctgcca
     1321 gagcgtgatc cccgccaccc cgaggatgac gatggcggca gccagggccg cgccggacgg
     1381 gcgtctcacc cgtccattgt tgcgtgcgcg cccctcgaac ggtggtcagg gcgaacggtg
     1441 gtcaggcggc gtgcggagac gcggtcagcg gcggccgacg gcggccagct cgagcacgtg
     1501 gccgaggccc agccgctgtc catcgccagc ccaccaccgg gtctcgcagt gcgagcagct
     1561 ccgcatgacc acttgcttgt cggccacgca cagcgagatc tccaccagct ttccgtccct
     1621 gcacaacggg cagtccatcg tcgaggcctc cgaggcgtgg cggtgtcgct ccgccgtcct
     1681 tccatcttcg gcccgacggc gccaaagctt gagcccggcc gggatctcca ttcaacgcaa
     1741 cccggcgagg acgaccacgg tcgcggcgtt gaaggtcatg tgggccacga tgcccggacc
     1801 gagacggccg ctgcggtggg ccagggcgcc gagcacgatg ccgaaggcga ccagggcgag
     1861 cagctgcaac gcctcgaaat gggccaggcc gaagaacacg gccgcggctc ccacgcccac
     1921 cggcgagggg tacagggcgc ccagcgaccg ctggaacagc ccccggaaga agatctcctc
     1981 ggcgatcgga gcgccgaccg cgatgagcgc accgagcacc acgaagccgg cgccgtgggc
     2041 gaccctggtc atctgcgtgg cggcatcgcc caggtgccgg tacgtgcgcg gcgcgagcag
     2101 ccggacgggc aggtacaggg cccagaccat ggggaactgg cagagcaggc cgacggccac
     2161 gccgacgggg aggtccgacg ggcggaagcg caagcccagc gccggcccca gcccaccacg
     2221 gggggcggcc accacgaccg ccgagccgac caggccgacc cagaggccga tcagcccggc
     2281 gatctccgcc ccgtagccgt tgagaccgga ccggtccccc gacaccgcga cccagagggc
     2341 ggcggccacc gagccgagca cggtcccgac caggaagccg ccgacggcga cgcccgcacc
     2401 ccaggcgggc aacggcggat cgggagcgct cagactgcga tctggggccg cagcgggatc
     2461 accggcgtgc gccggtcgtc ccgggcccac ccgatcggga cccgaagccc gtcggcgtgc
     2521 gccgagcaca tcgcccatgc tcccggctcg agctcctcgt ccatgctgtc gagccacacc
     2581 gtacggcgcg cgtagtcgta ggtgagcgtc gccgaggcgg acccgccaca gccggggcgc
     2641 acacatcggc gggcacgatc catagccggg cagcctaccg gcgggcggga cgcgaggggt
     2701 ggatgccgag ccgctccggg ttggtcccga gcgccgcgta ccatggctcc catgagccgc
     2761 caaccagggg acctgcgccc cggccgatcc ggcccacagg accgtcaggg ccggcctggc
     2821 cccccgggcg gagggcgggc cgggcccgag cagaactggc gttgggccgt gatcgcggtg
     2881 ctcgtgctcg tggtgctcgt gctgctgttc tcgggctcgc tcggcggggg caagtccaag
     2941 acgtcgatca gctacaacga cttcatcacc gacgtgtccc acgggcaggt cgactccgcc
     3001 acctacaaca acgacaccgg gtccatctcg ggcacgttga aggcgggggg ccgcttctcc
     3061 acgggcggcc cgcaccccgc catcccggcg gacgtgcaga ccatgcggga gaacggcgtc
     3121 aagctgacct tcaagaacaa ctcgagcaac gtcctcggct cgctgctcct gtacctgctg
     3181 ccgatcgtgc tgctcatcgg gttcttcgtg tacatgaacc gccgggccca gggccagatg
     3241 agcgggatca tgtccatcgg gcgctccaag gccaaggtgt acaccaccga gcggccccgc
     3301 acgacgttca acgacgtggc cggctacacc ggggtcaagc aggagatccg cgaggtggtc
     3361 gacttcctca agacgcccgc ccggttcaag gacatcggcg ccaagatccc caagggcgtc
     3421 ctgctggtgg ggccgcccgg caccggcaag acgctgctgg cccgggcggt ggcgggcgag
     3481 gcgggcgtgc cgttcatgtc cgtgaccggc tccgacttca tggagatgtt cgtgggggtg
     3541 ggcgccagcc gggtgcggga cctgttccag acggccagga agcaggcgcc ggcgatcatc
     3601 ttcgtcgacg agatcgactc catcggccgc aagcggggcg ccggcctcgg cggaggtcac
     3661 gacgagcgcg agcagaccct caatcagatg ctggccgaga tggacgggtt cgagaccacc
     3721 gagggtgtcg tcatgatggc cgccaccaac cgccccgaca tactcgaccc ggcgctgctg
     3781 cgaccgggtc ggttcgaccg ccagatcgtg gtgcccctcc ccgacctgga ggagcgcctc
     3841 ccgatcctcc aggtgcacag ccgcgacaag aaggtcggcc ccgacgtcga tctcaacctg
     3901 gtcgcccggg gcacgccggg catgagcggc gcggatctcg ccaaccttgt caacgaagcc
     3961 gccctccacg ccgtgcggcg gggcagcgac agcatccaga tgcaggactt cgaggccgcc
     4021 cgcgaccggg tgatcatggg ccagcgccgg gagacgatgg ccctgtcgat ggacgagaag
     4081 gagcgcatcg cctaccacga gggcggccac gccgtgctcg cctacgtgct cccgaccgcc
     4141 gaccccgtcc acaaggtcac catcctgccc accggcatgg ccctcggcgt gacccagcag
     4201 ctgcccatgg aggagcgcca catctacccc cgcgactaca tcgaggactc gctcgcggcg
     4261 cgcatggggg gccgggtcgc cgagatgctg acctacggcg acctgtccac gggcgccaac
     4321 aacgacctgg tcggcaacac cgagctggcc cgcaagatgg tgcgcgaatg gggcatgagc
     4381 gaggccatcg gccccatggc gtggggatcg cagggccaag tgttcctcgg cgaggacctc
     4441 atgcacaccc gcgactacag cgacgagacc agctgcgtca tcgacgagga ggtcgaacgc
     4501 atcctccgcg aacaagagga ccgggccacc aaggc
//
LOCUS       DASWGG010000310         4117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2848467, whole genome shotgun sequence.
ACCESSION   DASWGG010000310 DASWGG010000000
VERSION     DASWGG010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2848467"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VF954_05560"
     CDS             <1..574
                     /locus_tag="VF954_05560"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9530583.1"
                     /translation="GHGDPELHPDGRAQAELAADRLVHEDISAIYVTTLRRTVETAAP
                     LARRLGITPMVEADLREVHLGEWEGAAFRKHVAEGHPTALRMFQEQRWDAIPGAEAAG
                     TFAARVRAGIGRIASRHPEATVVVFTHGGVIGQILADAAGAARGFAFVGSDNGSISQL
                     VVVGDRWIIRRYNDTAHLGLGLSTAAQPLT"
     gene            complement(589..846)
                     /locus_tag="VF954_05565"
     CDS             complement(589..846)
                     /locus_tag="VF954_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530584.1"
                     /translation="MEDRPNSPPAAATAQDAAEAVVNAHAWLEANSGWSPPDGDTLAE
                     WEADGVCRCPDECLVTPRGWCAHGLASWRLVLDALSPGEPA"
     gene            complement(1555..2592)
                     /locus_tag="VF954_05570"
     CDS             complement(1555..2592)
                     /locus_tag="VF954_05570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9530585.1"
                     /translation="MKLSTSLAYGGDPKVAAAQARDLERAGIDMIWVAELYSFDAVSL
                     LGYLAAHTERAELASGILPLYSRTPTLTAMTAAGLDAVSNGRFVLGLGASGPQVIEGW
                     HGVPYDKPVQRTREIIDICRQVWRRERVVYDGEVYHLPLPPDQGTGLGKALKIINTPV
                     RPDIPVYIASLGAKNVQLTAELADGWLPAFFHPGKARQVFGADLDKGAGRRDPGLNPL
                     EIVAGGTVCVCDPATAEKLRNRGRPGTALYVGGMGAKGKNFYNNVFRRYGYESEAEKI
                     QDLYLSGQKAEAEALVPEEFLAATTLVGDEGQVREQIQAYRDAGVTRLSVRPVGEDVL
                     GLIEKVRGWVG"
     gene            complement(2757..3785)
                     /locus_tag="VF954_05575"
     CDS             complement(2757..3785)
                     /locus_tag="VF954_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03857 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX9530586.1"
                     /translation="MPAPLEQLGFYTLAGAPRSPRDLVGEVAAAEELGLGWAFISERF
                     NIKEASTLSGAVGAVSSSLTIATAATNHNTRHPIVTASYATTMHRLTGGRFVLGLGRG
                     IAPLMVALGLPPVTTAQLEDFASLMRRLWHGETILGHDGPAGRYPFLRLDPTFHEDIP
                     LALTAFGPNSLALGGRAFDHVVLHTFFTDETLARSVEAVKVAAEKAGRDPASVKVWSC
                     FATVGDHLPEPLRLKKTVGRLATYLQAYGDLLVATNRWDPEILARFRADPMVASFPGA
                     IDDKATTEQLQHVAGLLPAEWLAPSATGTPAQCVAKVQGQFDLGADGVILHGASPQEL
                     APILAAYP"
     gene            complement(3820..>4117)
                     /locus_tag="VF954_05580"
     CDS             complement(3820..>4117)
                     /locus_tag="VF954_05580"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9530587.1"
                     /translation="ASGAVFGLFGAAAMGLRQRGINVMQTNIGVLLVINLVFTFVIPG
                     ISVGAHLGGLAGGALCGGVMLRERPSPTATVRGVLVAAALTLAAGAGAVAIAHP"
BASE COUNT          589 a         1440 c         1510 g          578 t
ORIGIN      
        1 cggccatggc gacccggagc tgcatccaga tggccgggcc caggccgaat tggcggccga
       61 ccggctggtc cacgaggaca tctccgccat ctacgtcacg accctccggc gcaccgtgga
      121 gaccgccgcg cccctcgccc gccgactggg gatcaccccg atggtggaag cggacctgcg
      181 tgaggtccac ctgggggagt gggagggcgc ggcttttcgc aagcacgtcg cggaaggcca
      241 cccgaccgcg ctccggatgt tccaggagca gcggtgggac gccatccctg gcgcggaggc
      301 ggccgggacc ttcgccgccc gggttcgggc cggcatcggc cggatcgcgt cgcggcatcc
      361 tgaggccacc gtggtggttt tcacccacgg cggggtcatc gggcagatcc tcgccgacgc
      421 cgccggagcg gcacggggct ttgccttcgt cgggtcggac aatggctcga tctcccagct
      481 cgtggtcgtg ggcgaccgct ggatcatccg gcggtacaac gacacggcgc acctgggcct
      541 cgggttgtcg accgcagccc aaccgctcac gtagcggcgg tgtccgagtc aggcgggctc
      601 gccgggcgat agcgcgtcga gaaccagccg ccacgatgcg agaccgtggg cgcaccaccc
      661 gcgaggagtg accaggcact cgtcggggca cctgcacacg ccgtccgcct cccactccgc
      721 aagggtgtcc ccgtccgggg gcgaccaccc gctgttggcc tccaaccaag cgtgggcgtt
      781 gacgacggcc tccgccgcgt cctgggcggt ggcggcggcc ggtggcgagt tgggtcgatc
      841 ctccactctt ttgcacccta tggcgacttg ccctagtgga ggaatgggcc gacccccggc
      901 cacacctggt cggagggctg tccgacgagc aggtcgacga tgagactcga cgatgagact
      961 cgacaatggg atcgacaact atgtcgacaa tatgccgacc atgacgtcga ccgtcgatcg
     1021 cccctgacgt cgataatgat gtcggaaata acgccgtgcc ctcaggcaca tggctacccg
     1081 tggacggcca agatttcctt caacaacgga tggcgctggc aggtcggcgg ttgtaccgtt
     1141 gcacttgggg ggcgggggcc gctccgtccg ccgtggagcg gcccgccccc ttcattcacc
     1201 ccgacccggc gaccggttgc acggcgccca ccgcccgctg cggaggcaac tgcgctggcg
     1261 tccgtccgga gtggaggcac ccggttgtcg acgtgcccgg cgtcgcctga ccgggcgtga
     1321 ccgggtcgcg tgctgcggcg agccgctcac gctgcgtggc gggcgattct gggcgcgccc
     1381 aggaacacgg tgtgtaccaa accgcgccca gttcggggcg tgtcgcccga tgggtgtttc
     1441 tgggcgcgcc agtggacatc ccaggcaccc aaccgcgccc agttcggcgg agggacgcca
     1501 ctcccccacc gcccccgcgc ccccaccgcc ccctggctcg cgcccggcgg tggctcagcc
     1561 cacccagcca cgcaccttct cgatcaggcc gagcacgtcc tcgcccacgg ggcgcacgct
     1621 gaggcgggtc acgccggcgt ccctgtatgc ctggatctgc tccctgacct ggccctcgtc
     1681 gccgaccagg gtcgtggcgg ccaggaactc ctccggcacc agggcctcgg cctccgcctt
     1741 ctgcccggac aggtagagat cctggatctt ctcggcctcc gactcgtagc cgtaccggcg
     1801 gaacacgttg ttgtagaagt tcttgccctt ggcacccata ccaccgacgt acagggcggt
     1861 cccgggccgg ccgcgattgc gcagcttctc ggcggtggcc gggtcgcaga cacagaccgt
     1921 gccaccggcg acgatctcga gggggttcag gcccgggtcc cgacgccccg cccctttgtc
     1981 caggtcggcc ccgaagacct ggcgggcctt gcccggatgg aagaacgccg gcagccagcc
     2041 gtcggccagc tccgcggtca gctgcacgtt cttggcgccg aggctggcga tgtacacggg
     2101 gatgtcgggg cgcacgggcg tgttgatgat cttcagggct ttgcccaggc ccgtcccctg
     2161 gtcgggcggg agggggaggt ggtagacctc gccgtcgtac acgactcgct cgcggcgcca
     2221 gacctggcgg catatgtcga ttatctccct ggtccgctgc accggcttgt cgtagggcac
     2281 gccgtgccag ccctcgatca cctgcgggcc ggaggcgccg agaccgagca cgaagcggcc
     2341 gttggagacg gcgtcgagcc cggccgcggt catggcggtc agcgtcgggg tccgggagta
     2401 cagcggcagg atgcccgagg ccagctcggc ccgctcggtg tgggcggcga ggtagccgag
     2461 gaggctcacg gcgtcgaagc tgtagagctc ggcgacccag atcatgtcga tcccggcccg
     2521 ctcgaggtcg cgggcctggg ccgcggccac cttcgggtcc ccgccgtagg cgagcgacgt
     2581 cgaaagcttc atggcaacct ccccatctcg atgtgggccc acgctacgcc ggcacgtcgg
     2641 ccccctgcgg ggcgcgctcc ggccccctgc ggggcgcacg cataggcggc cagcccgcgg
     2701 cgcgctcccc gggggccggg ggaggcggcc ggtatcggca ccgccccgtc gagcgctcag
     2761 gggtaggcgg cgaggatcgg tgccagctcc tggggcgagg ccccgtgcag gatcacgccg
     2821 tcggcgccga ggtcgaactg gccctggacc ttggcgacgc actgcgccgg cgtgccggtg
     2881 gcggatgggg ccagccactc ggcgggcagg agcccagcga cgtgctggag ctgctcggtg
     2941 gtcgccttgt cgtcaatggc cccggggaaa gaggccacca tcgggtcggc ccggaaccgg
     3001 gccaggatct cggggtccca ccggttggtc gcgaccagaa ggtcgccgta agcctggaga
     3061 tacgtggcca ggcgaccgac ggtcttcttc agccgcagcg gctcgggcag gtggtcgccg
     3121 acggtggcga agcaagacca gaccttcacc gaggccgggt cgcggccggc cttctcggcg
     3181 gcgaccttga cggcctcgac gctgcgggcc agggtctcgt cggtgaagaa agtgtggagc
     3241 acgacgtggt cgaacgctcg gccgcccagc gccagggagt tgggcccgaa cgcggtcagg
     3301 gcgagcggga tgtcctcgtg gaaagtgggg tcgagccgca ggaacgggta ccggcccgcg
     3361 ggcccgtcat ggcccaggat cgtctcgccg tgccacagcc ggcgcatgag gctggcgaag
     3421 tcctcgagct gggcggtggt gaccggcggg aggcccagcg ccaccatgag cggtgcgatc
     3481 ccccggccca acccgaggac gaaccggccg ccggtgaggc ggtgcatggt ggtcgcgtac
     3541 gaagcggtga ctatcgggtg gcgggtgttg tggttggtgg ccgcggtcgc gatcgtgagg
     3601 ctggacgaca ccgcacccac cgcgcccgag agggtggagg cctccttgat gttgaagcgc
     3661 tccgagatga aggcccagcc caggcccagc tcctcggccg cggcgacctc gcccacgagg
     3721 tcccggggtg atcgcggggc cccggcgagg gtgtagaagc cgagctgttc gagaggagcg
     3781 ggcatcccgg ccacactaac ggttgcccac ggcgtggggt caggggtggg cgatggcgac
     3841 cgcgcccgcg cccgccgcca gcgtgagggc ggcggcgacc agcacgcccc gcaccgtggc
     3901 cgtcggcgag ggtcgctccc gcagcatgac acccccgcag agggcgccac cggcgaggcc
     3961 cccgaggtgg gcgccgacgg agatcccggg gatgacgaag gtgaacacga gattgatcac
     4021 cagcaggacc ccgatgttgg tctgcatgac gttgatgccc cgctgccgca ggcccatggc
     4081 cgccgcgccg aagagcccga agacggcgcc tgaggcg
//
LOCUS       DASWGG010000311         4514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3178481, whole genome shotgun sequence.
ACCESSION   DASWGG010000311 DASWGG010000000
VERSION     DASWGG010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3178481"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VF954_05585"
     CDS             complement(<1..182)
                     /locus_tag="VF954_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530588.1"
                     /translation="MAINLNIAPREGEAGDLGAHQDGAATFVSAMLTTLGEALGGFDE
                     RLARLEQAVGQALFEAG"
     gene            complement(221..1090)
                     /locus_tag="VF954_05590"
     CDS             complement(221..1090)
                     /locus_tag="VF954_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9530589.1"
                     /translation="MTSEAGGMAVADVGSGRAVLLLHGQPGLGRDWAAVSTLLDDRYR
                     LIAPDRPGYGGTGGAATGLFGNAAAAAGLLDELGIEEAVVVGHSLGGGIALAMAQRHP
                     ERVSALVLVASVGAPSSVDLLDRVLAWPVVGEMLAFVAFRYLGRFLKSPPALHRSNRF
                     APELAVLEPEKLRVRAARWQAERVWEAFSVEQRALVAELPVVVARMGDIRVPTVVVAG
                     RRDSIVGPAASAALAHAITGAELRWVDGGHLLTVEAPEAVAAAVTAAASLPPGAGPGE
                     GPDPPPPARGRVS"
     gene            complement(1174..1872)
                     /locus_tag="VF954_05595"
     CDS             complement(1174..1872)
                     /locus_tag="VF954_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9530590.1"
                     /translation="MDPATAEARVLVVDDEPYITDLIAAALRYEGFHVDVAGNGRAAL
                     ASAATGRHDLVILDVMLPDLDGLEVCRRLRADGESMPVLFLTARDATEDKVAGLTVGG
                     DDYVTKPFSLEELIARVRAILRRSRPEASRGRLQFADLVMDEDTHEVWRGDAPVELTA
                     TEFNLLRYLLLNSRRVLSKSQILDHVWQYDFGGDPNIVETYISYLRKKVDRHLPPLIH
                     TIRGVGYSLRLPRS"
     gene            complement(1947..2888)
                     /locus_tag="VF954_05600"
     CDS             complement(1947..2888)
                     /locus_tag="VF954_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9530591.1"
                     /translation="MEHRKLGSLDVSLVGLGTNNFGMRCDEEQSAAVVRAALDAGVTF
                     FDTADGYGGTKSEGYLGRALAGVRDEVVIATKFGPPIGGEGTGGGSARWVRQAVEDSL
                     RRLGTDRIDLYQQHFPDTAVPIEETLGALNELVEAGKVREIGNSNYSAEQIRQADAAA
                     RAHGWARFVSAQNELNLLRRQPLVEVVPACEQAGLGFLPYFPLASGLLTGKYRRGQDP
                     PADSRLALMPEDRRAQAFTDENFDLVEGLTAFAADHGHSLLDLAFAWLAGQPTIASVI
                     AGATRPEQVRANAAAAAWTLGPSDLSEVQAVIDRNRS"
     gene            3035..>4514
                     /locus_tag="VF954_05605"
     CDS             3035..>4514
                     /locus_tag="VF954_05605"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9530592.1"
                     /translation="MATSVLIVSASMGAGHDGAARELAARLEARGHATKVVDFMACAP
                     LGIGTLVRVLYELQLRVAPWAYEATYRVWYLLPLLISPVVRFVSFLTSRRVRRHIREI
                     DAGLVVTTYPLASLTLGRMRARGQVDVPVSTFVTDFAVHPLWVHPGVDLNVCVHPQSA
                     ARAAQMTQRPSCAPGPLVPDRFRRNATSRAAARRQLGLPDDETIVLVVAGSWGVGDLE
                     GTFADLLATGRYYPVAVCGRNDRLKGRLERQRGGRALGWTDQMPTLMAAADAIVQNAG
                     GLTCMEAFAAGLPVVSFRPIPGHGRENAIEMERAGVAGFARSPGELVAILDRATTLAG
                     RRMVGRARAMFTGDATDHVLALGPATEDAAGEEALPLAAGAEDLAGPMAARAGHRRRR
                     GRRVGMAAAAMALAYTGVNVAADAATAMGYAGGHADRHSAGAYVAVRLGPSALLDPGT
                     GPRLAASHISAIIGGTLAARHPAEVRALKAAGVDVVNGGWGRP"
BASE COUNT          583 a         1680 c         1646 g          605 t
ORIGIN      
        1 ccggcctcga agagggcctg gccgaccgcc tgctccaggc gagccagccg ctcgtcgaag
       61 ccgccgagcg cctcgcccag ggtcgtgagc atcgccgaga cgaacgtcgc cgccccgtcc
      121 tggtgcgcgc cgagatcgcc cgcttcgccc tcacggggtg cgatgttgag gttgatggcc
      181 atgcaccctc ctgccgcccg ggtcggggct cagtgggttc ctacgatacg cggccgcggg
      241 ccggcggcgg cggatccggg ccctcacccg ggccggcgcc gggtgggagc gatgccgccg
      301 cggtgaccgc ggcggccacg gcctccggcg cctcgacggt cagcaggtgg ccgccgtcga
      361 cccaccgcag ctccgccccg gtgatggcgt gggcgagcgc cgccgaggcc gccgggccca
      421 cgatcgagtc cctgcggccc gccaccacga ccgtcggcac ccgaatgtct cccatgcggg
      481 cgacgacgac cggcagctcg gccaccaggg cccgttgctc gacggagaac gcctcccaca
      541 cccgctcggc ctgccagcgg gccgcgcgca cacgaagctt ctcgggctcc aggacggcca
      601 gctcgggcgc gaaccggttc gaccggtgga gggccggcgg cgacttcagg aaccggccca
      661 ggtaccggaa cgcgacgaag gccagcatct cgccgaccac cggccaggcc agcacccggt
      721 cgagcaggtc gaccgaggac ggggcgccga cggaggccac caggaccagg gccgacaccc
      781 gctccgggtg gcgctgggcc atcgccagcg cgatgccgcc gccgaggctg tggccgacca
      841 ccaccgcctc ttcgatgccc agctcgtcga ggaggccggc ggcggcggcg gcattcccga
      901 acaggccggt ggccgcgccg ccggtgcccc cgtagcccgg tcggtccggg gcgatgagcc
      961 ggtagcggtc atccagcagc gtggacaccg ccgcccagtc gcggcccagg ccgggctggc
     1021 cgtggaggag caggaccgcc cgcccggagc ccacgtccgc cacggccatc ccgcctgcct
     1081 ccgaggtcat ggcccgatga cgctcatggc ggggggccca tggcaggatg ctcgtggcgg
     1141 gatgctcgtg gaggggtgct cacgaccggg tgctcacgac ctgggaagcc gcaggctgta
     1201 ccccacgccc cggatggtgt ggatgagggg cgggagatgc cgatcgacct tcttgcgcag
     1261 atagctgatg taggtctcca cgatgttggg atctcccccg aagtcgtact gccagacgtg
     1321 atccaggatc tgcgacttgg acagcacccg ccgggagttg agcaggaggt aacgcagcag
     1381 gttgaactcc gtggcggtga gctcgaccgg cgcatccccg cgccacacct cgtgggtgtc
     1441 ctcgtccatc accaggtcgg cgaactggag ccgcccgcgg ctggcctccg ggcgggaccg
     1501 gcggaggatg gcccggaccc gggcgatcag ctcctccaag ctgaagggct tggtgacgta
     1561 gtcgtccccg ccgacggtga ggccggccac cttgtcctcg gtcgcgtcgc gggccgtgag
     1621 gaacagcacc ggcatcgact cgccgtcggc tcgcagccgg cggcacacct cgagcccgtc
     1681 gagatcgggg agcatcacgt ccaggatgac caggtcgtgg cgtccggtcg cggcgctggc
     1741 cagggcggcc cggccgttgc ccgccacgtc cacgtggaac ccctcgtagc gcagggcggc
     1801 tgcgatgagg tcggtgatgt acggctcgtc gtcgacgacg agcaccctgg cctcggcggt
     1861 ggcgggatcc atcaccctcc atgctgcctg ctggcgctgg gaagttcatg tgaggcgcgg
     1921 gcggcgcctt cttggacggg gcgagctcag gaccggttgc ggtcgatgac ggcctggacc
     1981 tcggacaggt cgctgggccc cagcgtccac gccgcggccg ccgcgttggc ccggacctgc
     2041 tcgggccggg tcgctccggc gatgaccgac gcgatggtgg gctggcccgc cagccacgcg
     2101 aacgccaggt ccagcaggga gtggccgtgg tcggcggcga aggcggtcag gccctcgacc
     2161 aggtcgaagt tctcgtcggt gaacgcctgg gcgcggcggt cctcgggcat gagggcgagg
     2221 cggctgtccg ccggcgggtc ctggccgcgc cggtacttgc ccgtcagaag gccgctggcc
     2281 agcgggaagt agggcaggaa gcccaggccg gcctgctcac acgccggcac cacctcgacg
     2341 aggggttggc gccgcagcag gttcagctcg ttctgggccg atacgaaccg ggcccagccg
     2401 tgcgcccgag cggcggcgtc ggcctgccgg atctgctcgg cggagtagtt ggagttgccg
     2461 atctcccgga ccttgcccgc ctccaccagc tcgttgagag ccccgagcgt ctcctcgatc
     2521 gggaccgcgg tgtcgggaaa gtgctgctgg tagagatcga tgcggtcggt gcccagccgg
     2581 cgcaggctgt cctcgacggc ctggcggacc cagcgggcgc tgccaccacc cgtgccctcg
     2641 cccccgatcg gggggccgaa cttggtggcg atgaccacct cgtcccggac gccagccagg
     2701 gcccgcccca ggtacccctc cgacttggtc ccgccgtagc cgtcggcggt gtcgaagaag
     2761 gtgacgcccg catccagggc cgcacgcacg accgcggccg actgctcctc gtcgcagcgc
     2821 atcccgaagt tgttggtgcc caggccgacc agcgacacgt cgagcgatcc cagcttgcga
     2881 tgttccatcg tcacctccgt gaccgcttct tgacccgcgg gtcatgtcga cagggatatt
     2941 acgaggagcc gggatgacag cgccatgccg cgccatcggt tggaccccgg catgcctggc
     3001 tttcagcttt ctctcaggta ccctctgcgt ccgtatggcc accagcgtcc tgatcgtcag
     3061 cgccagcatg ggcgccggcc acgacggggc ggcgcgcgag ctggcggcgc gcctcgaggc
     3121 gcgcgggcac gccaccaagg tcgtcgactt catggcctgc gccccgctcg gcatcggcac
     3181 cctggtgcgg gtcctctacg agctgcagct ccgggtcgct ccctgggcct acgaggccac
     3241 ctaccgcgtg tggtacctgc tgccgctgct gatctcgccg gtcgtgcggt tcgtgagctt
     3301 cctcaccagc cggcgggtgc gccgccacat ccgggagatc gacgccgggc tggtcgtgac
     3361 cacgtacccc ctggcctcgc tgaccctcgg gcgcatgcgc gccaggggcc aggtcgatgt
     3421 cccggtgtcc acgttcgtca ccgacttcgc cgtgcacccc ctgtgggtcc acccgggcgt
     3481 cgacctcaac gtctgcgtcc acccccagtc ggccgcccgg gccgcacaga tgacgcagcg
     3541 cccgagctgc gcgcccggcc cgttggtccc ggaccgcttt cggcgcaacg ccacgagccg
     3601 ggcggccgcc cgcaggcagc tcggcctgcc cgacgacgag acgatcgtcc tcgtggtggc
     3661 cggctcgtgg ggggtcggtg acctggaggg caccttcgcg gacctgctcg ccaccgggcg
     3721 ctactacccc gtcgccgtgt gcggccgcaa cgaccggctc aagggccgtc tcgagcgcca
     3781 gcggggcggc cgggccctcg gatggaccga ccagatgccc accctgatgg ccgccgccga
     3841 cgccatcgtg cagaacgcgg ggggcctcac ctgcatggag gccttcgccg caggcctccc
     3901 ggtcgtcagc ttccggccga tccccgggca cggccgggag aacgccatcg agatggagag
     3961 ggccggcgtg gccggcttcg cccgctcgcc cggcgagctg gtggccatcc tcgaccgggc
     4021 gacgaccctc gccgggcggc ggatggtcgg tcgggcccgg gccatgttca cgggcgacgc
     4081 gaccgaccac gtgctggccc tggggccggc gaccgaggac gcggcaggcg aggaggcgct
     4141 gccgctcgcg gccggggccg aggatctcgc cggcccgatg gcggcccggg ccgggcaccg
     4201 gcgtcgtcgc ggccgacggg tgggcatggc cgcggccgcc atggcgctcg cctacaccgg
     4261 cgtcaacgtg gctgccgacg ccgcgaccgc catgggctac gccggcggcc acgccgaccg
     4321 ccactccgcc ggggcgtatg tggcggtgcg cctcgggccg agcgcgctgt tggatccggg
     4381 gaccggtccg cgcctcgccg cctcgcacat ctccgccatc atcggcggca cgctggccgc
     4441 ccgccacccg gccgaggtcc gcgccctcaa ggccgcgggc gtcgacgtcg ttaacggcgg
     4501 gtggggccgc ccga
//
LOCUS       DASWGG010000312         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_453863, whole genome shotgun sequence.
ACCESSION   DASWGG010000312 DASWGG010000000
VERSION     DASWGG010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453863"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VF954_05610"
     CDS             <1..440
                     /locus_tag="VF954_05610"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX9530593.1"
                     /translation="DWLFAALWRAGYANQPTSVSADDGLRLSDAYVAAAVRCAPPANK
                     PTPAERDRCLPYLGRELALLGRVRVVVALGQFGYDAVARLLEVQKRPSFGHGVEAAAA
                     EGGRTIICSYHPSQQNTFTGKLTEPMLDGVFARARQLTSAGGR"
     gene            complement(418..1227)
                     /locus_tag="VF954_05615"
     CDS             complement(418..1227)
                     /locus_tag="VF954_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9530594.1"
                     /translation="MLAGLATGAMSGAIGIGGASLSTPAVRLLGASAYLAVGTTLPSI
                     FPGAAVGTVRFVRGGLVDARAVLWSVPPGLLGAVAGSLASHALPGHGRALMIATAVLL
                     AVSAVRIARSSPPEAAPGGLAAVPAGDGPDGGGPRASNGGGPPRPWVLGAIGLGAGLL
                     SGLLGVGGGTVLVPGLVELGRYPIKTAVATSLVCVGLLAIPSTVAHAALGDIDWRFAA
                     FLTLGVMPGARMGAAGAVRAGEGRFRLAVAGFLGMLALFFGAGEIIALLRR"
     gene            1304..1786
                     /locus_tag="VF954_05620"
     CDS             1304..1786
                     /locus_tag="VF954_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9530595.1"
                     /translation="MPQAPDHPRLCDRLKSTVRDLVALTANVGPARLRRAPGPDEWSA
                     AMVIAHLADSEMAYGVRLRLLLTEPRPVLAPFDDNAWVERFSSLDQDPKDTLTRWRVL
                     REANVRIFESLEDTEWKQAGVQVERGEQTVEQIAVLLADHDRNHLDQIRRTLAEGGRG
                     "
     gene            complement(1783..>2412)
                     /locus_tag="VF954_05625"
     CDS             complement(1783..>2412)
                     /locus_tag="VF954_05625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9530596.1"
                     /translation="AEATAKGYLRSQIMPIEIAANGHAAEVMDRDEGIRPNSTYEAVS
                     ALPPAFNPEHTITAGNSSQITDGAAAVLLMSKEKAAELGLKPRARIIASSVSGVAPEI
                     MGVGPIEAVTRVLRQAGMTIKDIDVVELNEAFAAQVLPVCDELGISIEDQLNPHGGAI
                     ALGHPFGMTGARIMTTLINDLQTLDKTYGVETMCIGGGQGMAMIIERLN"
BASE COUNT          356 a          878 c          868 g          310 t
ORIGIN      
        1 gcgactggct cttcgccgcc ctgtggcggg ccgggtacgc caaccagccc acgagcgtct
       61 cggccgacga cggactgaga ctcagcgacg cctatgtcgc cgctgccgtg cgatgcgccc
      121 caccggccaa caagccgaca ccggccgagc gggaccgctg cctgccctac ctcggccgcg
      181 agctcgccct gctgggccgg gtccgggtcg tggtggcgct cggccagttc ggctacgacg
      241 ccgtcgcccg gttgctggag gtccagaagc gaccgtcctt cggccatggt gtggaggccg
      301 ccgccgcgga gggcggtcgc acgatcatct gctcgtacca cccgagccag cagaacacct
      361 tcaccggcaa gctgaccgag cccatgctcg acggggtctt tgcccgggcg cggcagctca
      421 cctccgcagg agggcgatga tctcgccggc cccgaagaag agggcgagca tcccgaggaa
      481 gccggcgacg gcgaggcgga agcggccctc tcccgcccga acggcgccgg cggcgcccat
      541 gcgggcgccc ggcatgacgc cgagcgtgag gaaagccgcg aagcgccagt cgatgtctcc
      601 gagcgcggcg tgggcgacgg tggagggaat ggcgagcagg ccgacgcaca ccaggctggt
      661 ggcgaccgcg gtctttatcg ggtagcgacc cagctcgacg aggccgggca cgagcacggt
      721 gccgccgccg acgcccagca gccccgacag caggccggcg cccaacccga tggcgcccag
      781 cacccaggga cgcggtggac cgcctccgtt tgaggcccgc ggcccgccgc cgtcgggccc
      841 gtctcccgca ggcactgccg ccagcccgcc gggcgcggcc tccgggggcg acgaccgggc
      901 gatgcgcacg gccgagacgg cgaggagcac ggcagtggcg atcatcagcg cacgcccgtg
      961 gcccggcagg gcgtgcgacg ccagcgaacc ggccacggca cccaggaggc cgggtggcac
     1021 ggaccacagc acggcgcgag cgtcgaccag cccgccccgc acgaaccgga ccgtgcccac
     1081 ggccgcgccg gggaagatcg agggcaaggt ggtcccgacg gcgagatagg ccgacgcgcc
     1141 caacagccgg acggcggggg tcgacagcga cgccccaccg atgccgatgg cgcccgacat
     1201 tgcacccgtg gccaggcccg cgagcatggt caatccgtcg cgaacgggtc ccgggtgcac
     1261 cgcctgcttg tctagccgct cccccgcgtg ggaagttcag gccatgccgc aggcccccga
     1321 ccatccccgc ctctgtgatc gcctcaagtc cacggtgcgc gacctggtcg ccctgacggc
     1381 aaacgtggga ccggcgcggc tgcggcgggc gccggggccc gacgagtggt ccgcggccat
     1441 ggtgatcgcc cacctcgccg actcggagat ggcctacggc gtgcggcttc gcctcctgct
     1501 caccgagccc cgccccgtgc tggccccctt cgacgacaac gcctgggtgg aacgcttctc
     1561 ctcgctggac caagacccca aggacaccct gacgcggtgg cgggtcctgc gggaggccaa
     1621 cgtccggatc ttcgagtcgc tggaggacac ggagtggaag caggcgggcg tccaggtcga
     1681 gcgcggtgag cagaccgtgg agcagatcgc ggtcctgctc gccgaccacg accgcaacca
     1741 tctcgaccag atccgccgca ccctggccga gggcggccgc ggctagttga gccgttcgat
     1801 gatcatggcc atgccctggc ccccgccgat gcacatcgtc tcgaccccgt aggtcttgtc
     1861 cagcgtctgc aggtcgttga tgagcgtcgt catgatccgg gcgccggtca tgccgaacgg
     1921 gtggcccagg gcgatggcgc cgccgtgggg gttgagctgg tcctcgatgg agatcccgag
     1981 ctcgtcgcac accgggagga cctgggccgc gaaggcctcg ttaagctcga cgacgtcgat
     2041 gtccttgatg gtcatcccgg cctggcgcag caccctggtc acggcctcga tcgggcccac
     2101 gcccatgatc tccggcgcga cgcccgagac gctcgacgcg atgatccgcg cccgcggctt
     2161 gagcccgagc tcagcggcct tctccttcga catcaggagc acggcggcgg cgccgtccgt
     2221 gatctgcgag gaattgccgg cggtgatggt gtgctcgggg ttgaaagcgg gcggcagggc
     2281 ggacaccgct tcgtaggttg agttggggcg gatgccctcg tcgcggtcca tgacctcggc
     2341 ggcgtgcccg ttggcggcga tctcgatggg catgatctgc gagcgcaggt agcccttcgc
     2401 ggtcgcctcg gc
//
LOCUS       DASWGG010000313         3470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2849648, whole genome shotgun sequence.
ACCESSION   DASWGG010000313 DASWGG010000000
VERSION     DASWGG010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2849648"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1602
                     /locus_tag="VF954_05630"
     CDS             247..1602
                     /locus_tag="VF954_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX9530597.1"
                     /translation="MDFETGHFSHRWHHHALGHQHPAGEVAQVGHERDDLGRMGVVLS
                     AGRAAGGLAALAWTVYAARRLQGDWGQLSAVLAYASVASIGTDLGIPLALTRLACRHP
                     TLDRAAVEAAIRRRAAAGTLAAGALILAWVNTKSGAGRWGLAAVYGISVTVTPVTSSY
                     LALLRGRANAGIEAIYDVVRQVALPALGIAAIETGLGVFGVLGAYVAVDAASAAVIAR
                     LARGRLRFVPGPDAGEEDELRLRSTVPLSATTIVGSAYERVDSAMLAPLAGTAAIGIY
                     RMISPIYGAVLMPARALGDTAAVGAGRGDGGASGTTRATVAPFALRAAVVTVPLAVLL
                     AVVGPPLLPHVLGVKASVHNPHPINWSRAATPLRILMAATVPSAALAVLTPVAVMARR
                     GRVFDLALGALGANIVMNLALVPHWAAGLGASGAALAFLITETALAAALWAGLPAKAV
                     S"
     gene            1613..3307
                     /locus_tag="VF954_05635"
     CDS             1613..3307
                     /locus_tag="VF954_05635"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEX9530598.1"
                     /translation="MDVLVVVGDGEPPQRLEVRARYIRRHRRGDEVASLVGDLRARIP
                     RRVGVWGPLGEAVPIAAELHRAGLPTELFTDDTVPERERASLAGVDVRRVADPGPPMD
                     VAESLLDLIGRTPMVRLDRTARNLECTLVAKLELFNPGGSSKDRPARLMIEQAERDGR
                     LRPGGTIVEPTSGNTGVGLAIVAARRGYRCIFVCPDKVAADKVALLRAYGAEVVVCPT
                     SVPPEHPESYYSVSDRLVGEVPGAWKPDQYHNPDNPRAQYETAGPEIWEQTRGRVTHF
                     VAGVGTGGTVSGIGRYLKERDPAVQVIGADPEGSVYSGGSGRPYLVEGIGEDFWPSTY
                     DPSVVDAVVAVSDAESFATARRVTREEGLLLGGSGGTAVAAALRVGRDLPADAVVVVH
                     IPDSGRGYLSKLYNDAWMADYGFLHAAGATVGELLDARDPSLPALVHTHPEETVRQAI
                     SILHEYSVSQMPVVKHEPPVVVAEVVGGVSERELMDRAFADPAVLDRPVGEVMGAPLP
                     TIGAGEPVSVAVERLEGASAVLVLDRGHPIGVVTRSDVLFALAAGPHAPRAPGGRS"
BASE COUNT          412 a         1285 c         1304 g          469 t
ORIGIN      
        1 tggcccccgg ccagcacccg ggcctcgtcg ccgtgctcgg ccaggagcga gagcacctcg
       61 tccacggaga ccggcgcccg gtaggcgaac ggcggcagct tcatgagacc cgcggacgcc
      121 acatggcggg cgagcgtaac acctgggccg tgccgtcctc cgcgcccggg cggccaggtc
      181 tcctatcctt cgcagccgtg accgtgcgcc cggcccatcc gcagcccctg ccggtctagg
      241 cctcggatgg acttcgagac cggccacttc agccaccgct ggcaccacca cgccctcggg
      301 caccagcacc ccgcgggaga ggtggcgcag gtcggccacg agcgcgacga cctgggccgc
      361 atgggtgtgg tgctctccgc cggccgggca gctgggggcc tggccgcgct tgcctggacg
      421 gtgtacgccg cccggcgcct ccagggggac tggggccagc tgtccgccgt gctcgcctac
      481 gcctcggtcg cgtccatcgg caccgacctc ggcatcccgc tcgccctcac ccgcctggcg
      541 tgccgccacc cgacgctcga ccgggccgcc gtcgaggcgg ccatccgtcg ccgggcggcg
      601 gccggcaccc tcgccgccgg ggccctgatc ctggcgtggg tgaacaccaa gagcggcgca
      661 ggccgctggg gactggccgc cgtctacggc atctccgtga cggtcacccc ggtgaccagc
      721 tcctatctgg ccctcctgcg gggccgggcc aatgccggca tcgaggccat ctacgacgtg
      781 gtccgccagg tcgccctgcc ggcgttgggg atcgccgcca tcgagacagg gctcggcgtg
      841 ttcggggtgc tcggcgcgta cgtggcggtg gacgcggcat cggcggcggt catcgcccgc
      901 ctggcccgcg gccgccttcg gttcgtaccg gggcccgacg ccggcgagga ggacgagctg
      961 cggctgcgct cgacggtgcc cttgtcggcg acgaccatcg tcggcagcgc gtacgagcga
     1021 gtcgactcgg ccatgctggc ccccctcgcc ggcaccgcgg ccatcgggat ctacaggatg
     1081 atcagcccca tctacggcgc ggtgctcatg cccgcccggg ccctcggcga caccgccgcc
     1141 gtcggtgccg ggcgtggcga cgggggcgcg tccggcacga cccgggccac agtcgcgccc
     1201 ttcgccctcc gggcggcggt cgtcaccgtc ccgctggcgg tgctgctggc ggtcgtcggg
     1261 ccgccgctgc tgcctcacgt cctcggcgtg aaggcgtccg ttcacaaccc gcaccccatc
     1321 aactggagcc gggccgccac gcctctgcgg atcctgatgg ccgccaccgt gccgtccgcg
     1381 gctctcgccg tgctcacccc ggtcgcggtg atggctcgtc gaggaagggt gttcgacctg
     1441 gccctgggcg cgctcggcgc caacatcgtc atgaacctgg cgctggtccc tcactgggcc
     1501 gcggggctgg gcgcgagcgg cgccgccctg gccttcctga tcacggagac ggcgctggcc
     1561 gcggcgctgt gggccgggct gccggcgaag gcggtgagct agacagaggc ccatggacgt
     1621 gctggtggtc gtgggcgacg gcgagccgcc gcagcggctc gaggtccggg cccggtacat
     1681 ccggcggcac cggcgaggcg acgaggtggc gagcctggtg ggcgacctcc gggcgcgcat
     1741 cccccggcgg gtgggcgtgt ggggccccct cggcgaggcc gtgccgatcg ccgccgagct
     1801 gcaccgggcg gggttgccga cggagctgtt caccgacgac accgtgccgg agcgcgagcg
     1861 ggcgtcgctc gccggcgtag acgtgcgccg ggtggccgac ccgggcccgc cgatggacgt
     1921 ggccgagtcg ttgctcgacc tgatcggccg tacgccgatg gtccgcctgg accgcacggc
     1981 gaggaacctc gagtgcaccc tcgtggccaa gctcgagcta ttcaaccccg gggggtcgtc
     2041 caaggaccga cccgcccggc tgatgatcga gcaggccgag cgggacgggc ggctccggcc
     2101 gggcggcacg atcgtcgagc ccacgagcgg caacaccggc gtgggactgg cgatcgtcgc
     2161 cgcccggcgc ggctaccggt gcatcttcgt gtgcccggac aaggtcgcag ccgacaaggt
     2221 ggccctgctg agggcgtacg gggccgaggt ggtggtctgc ccgacatcgg tgccacccga
     2281 gcaccccgag tcgtactact ccgtgtccga tcgcctggtg ggggaggtcc cgggcgcgtg
     2341 gaagcccgac cagtaccaca accccgacaa cccgagggcc cagtacgaga ccgccggccc
     2401 ggagatctgg gagcagacca ggggccgggt cacgcacttc gtggccggcg tcggcacggg
     2461 cgggaccgtg tccgggatcg gccggtacct gaaggagcga gatccggccg tccaggtcat
     2521 aggcgccgac ccggagggct cggtgtactc gggggggagc ggccggccgt acctggtcga
     2581 gggcatcggt gaggacttct ggccgtcgac ctacgacccg tcggtcgtgg acgccgtcgt
     2641 cgcggtgtcc gatgccgaga gcttcgccac cgcccgccgg gtcacccggg aggaggggct
     2701 gctgctcggc ggctctgggg gcacggcggt ggcggccgcc ctgcgggtcg ggcgggacct
     2761 gcccgccgac gccgtcgtgg tggtgcacat ccccgactcc ggccggggtt acctctccaa
     2821 gctgtacaac gacgcgtgga tggcggacta cgggtttctc cacgccgcgg gggcgacggt
     2881 cggggagctg ctggatgccc gggacccttc cctgcccgcc ctcgtccaca cccatcccga
     2941 ggagaccgtg cgccaggcca tctcgatcct gcacgagtac tcggtgagcc agatgccggt
     3001 cgtcaagcac gagcccccgg tcgtggtcgc cgaggtcgtc ggcggggtga gcgagcggga
     3061 gctgatggac agggccttcg ccgaccccgc cgtcctcgac cggccggtcg gcgaggtgat
     3121 gggtgcgccg ctgcccacca tcggggccgg cgagcccgtg agcgtcgccg tcgagcggct
     3181 ggagggggcc tccgccgtgc tggtgctcga ccgcggccat cccattggcg tggtcacgcg
     3241 gtccgacgtg ctgttcgcgc tggctgccgg gccgcacgcg ccccgcgccc ccggtggccg
     3301 gtcgtgagcg agcgccccga gaccgacggg tggggcttcg agacccgggc catccacggc
     3361 ggcaccggcc cggacccggc aagcggggcg gtcgtcacgc cggtccacct ttcctccacc
     3421 ttcaggcagg atgccgtcgg cgtccatcgg ggctacgagt acgcccgctc
//
LOCUS       DASWGG010000314         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_130716, whole genome shotgun sequence.
ACCESSION   DASWGG010000314 DASWGG010000000
VERSION     DASWGG010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130716"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VF954_05640"
     CDS             complement(<1..662)
                     /locus_tag="VF954_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530599.1"
                     /translation="MGTIRFTEGGDDGLYDHAGHVAQIWDDGTIHGGGLVEEVDRRTV
                     ALRAACSCGWLGETVLPRDPADPDGAWREWEQVPGLEAEWVAHTDPLLQELGREARKR
                     RAGEPSAADLAGRIGDDVRALRDTLGGVSRLEAPQDVSHIVSSLADVPGRIANVLREC
                     DRWLRRGIEDFAADDGDDPAAWVLDAVEAMEAAAGHLAEAAAPLHRAAADLSHLALTP
                     EAA"
     gene            complement(664..987)
                     /locus_tag="VF954_05645"
     CDS             complement(664..987)
                     /locus_tag="VF954_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530600.1"
                     /translation="MNPTSAPKPPWPAPKPLLPPGPGILLPGASGDVDTGKIRRQLEV
                     PERATEAPLTALEGAEAVARQAGRLDRADRLGEVVRRVASAHASLAGALAALSEFERA
                     EKGKH"
     gene            complement(984..1364)
                     /locus_tag="VF954_05650"
     CDS             complement(984..1364)
                     /locus_tag="VF954_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530601.1"
                     /translation="MNPHGAKETGLRGGDLQTQVAKGWMNGHGRDTARILGTIAEQLR
                     DVAAFAGEGITREPFASVQFSAAGLALLVAAQAFRAAAEVTTAGPDGDLSEAEAMSVE
                     MVRLAHELGDYVANLKARFPGGQR"
     gene            complement(1460..1729)
                     /locus_tag="VF954_05655"
     CDS             complement(1460..1729)
                     /locus_tag="VF954_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530602.1"
                     /translation="MTDKPTARPALRPRPHPLDVEPPALPAPPVQAVAAPLPAPKTAL
                     NARIDLYIHGAVRHAALDLRRTVAEITEEALGEWLERNGRGVTRP"
     gene            complement(1734..2516)
                     /locus_tag="VF954_05660"
     CDS             complement(1734..2516)
                     /locus_tag="VF954_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9530603.1"
                     /translation="MDNVWAITNQKGGVGKSSTALHLAHALSTAGRRVLLVDGDPQAT
                     STATTNTEPADGVLSMFDVLSGTAKIAEVIVSAPTWGFDVAPAELAMARLEQVSALGS
                     EFRLAEALTGVADRYDLVLVDCPPSLGRLALAALVASTRALIVTEPSAPALKGVGDLL
                     ETLDVVAKRYAPGLALAGVVVNRVTPTREANLRLEELRAFFGDGQVWEPLIPQRTAVS
                     EAMGAHCPVTALTGDGARAAAEVFATLAARLQASTQATKEGE"
BASE COUNT          343 a          938 c          925 g          388 t
ORIGIN      
        1 gccgcctccg gtgtgagggc gaggtgggac agatccgcgg ccgcacggtg cagcggcgct
       61 gccgcctcgg cgaggtggcc ggctgcggcc tccatcgcct cgaccgcgtc aaggacccag
      121 gccgcagggt cgtcaccgtc gtcggctgcg aagtcctcga ttccgcggcg gagccaccgg
      181 tcgcactccc ggaggacgtt ggcgatgcgc ccgggcacgt cggccagcga gctgacgatg
      241 tgcgagacgt cctgcggggc ctcgagccgg ctgacgccgc ccagcgtgtc ccgcagtgca
      301 cggacgtcgt ctccgatgcg cccggccaga tccgcagcgg acggctcccc cgcccggcgc
      361 ttgcgcgcct ccctgccgag ctcctggagc agcgggtcgg tgtgtgccac ccactcggcc
      421 tcgagtcccg gcacctgctc ccactcccgc caagccccgt cggggtccgc cgggtcccgg
      481 ggaaggaccg tctccccgag ccagccgcag gagcaggccg cccgaagcgc cacggttcgt
      541 cgatcgacct cctcgacgag gccaccgccg tggatcgtac cgtcatccca aatctgggca
      601 acgtggccgg catggtcgta cagcccgtcg tcgccgcctt cggtgaagcg gatggtgccc
      661 atatcagtgc ttccctttct cggctcgttc gaactcggac agcgccgcga gggcgccagc
      721 cagggaggcg tgggcgctgg cgacgcggcg caccacctcg ccgaggcggt cggctcggtc
      781 gagacggccc gcttgacggg cgaccgcctc ggcgccctcc agggcggtca aaggggcctc
      841 ggtcgcgcgc tcgggcacct cgagctggcg gcggatcttg ccggtgtcga cgtcaccgga
      901 tgcaccgggc agcaggatgc cggggccagg cgggagtagc ggcttcggcg ccggccaagg
      961 tggcttcggc gcggaggtcg ggttcatcgc tgccctcccg gaaaccgggc cttgaggttc
     1021 gcgacgtaat caccgagttc gtgggcgaga cggaccatct cgacggacat ggcctcggct
     1081 tcgctgaggt cgccgtccgg ccccgctgtc gtgacctccg ccgcagcccg gaacgcctga
     1141 gcagcaacca gcagggccag gcccgcggcc gaaaactgga ctgaggcgaa cggctctctg
     1201 gtgatgccct cgccagcgaa cgccgccacg tcccggagct gctcggcgat cgtgccgagg
     1261 atgcgcgcgg tgtcccggcc atgcccgttc atccacccct tcgccacctg cgtttggagg
     1321 tcgccgccgc gcaggcccgt ctccttcgct ccgtgggggt tcatcgggcg acggccgctg
     1381 tcgacgacga cgccgagaga ccccagcgcc cgggcgcagc tcgctgggcg acggtaaagg
     1441 ggccgggccg agacagcggt catggccggg tgactccgcg cccgttcctc tccagccact
     1501 caccgagggc ctcctcggtg atctcggcca ccgtgcgccg caggtcgagc gcggcgtgcc
     1561 tgaccgcgcc gtggatatag aggtcgattc gtgcgttcag ggcggtcttg ggagcgggca
     1621 ggggcgccgc aaccgcctgt accggcggtg cgggtagtgc tggcggctcc acgtcgagcg
     1681 ggtgtggcct ggggcgcagc gccggccgcg ctgtcggttt gtctgtcatg gcgctactcc
     1741 ccttccttcg tcgcttgcgt gcttgcttgc aaccttgccg ccaaggtggc gaacacctct
     1801 gccgccgccc gggcaccgtc gcccgtcagg gccgtcaccg ggcagtgcgc ccccatggcc
     1861 tcggagacgg cggtccgctg ggggatcagt ggctcccaca cctggccgtc cccgaagaac
     1921 gcccgcagct cctccaggcg caggttggcc tcacgggtcg gcgtgacccg gttgaccacg
     1981 acgccggcga gcgccagccc gggcgcgtac cgcttggcga cgacgtcgag ggtctcgagc
     2041 aggtccccga cgcccttgag tgccggggcg gagggctcgg tcacgatcag cgcccgggta
     2101 gacgccacga gggcggctag ggcgagacgg ccgagtgaag ggggacagtc gacgaggacc
     2161 aggtcgtagc ggtccgccac tcccgtcagg gcctccgcca gccggaactc gctgcctagg
     2221 gccgacacct gctcgaggcg ggccatggcc agctcggccg gggcgacgtc gaacccccac
     2281 gtgggggcag agacgatgac ctccgcgatc ttcgccgtgc cggacaggac gtcgaacatg
     2341 ctcagaacgc cgtctgccgg ctcggtgttc gtcgtggcgg tgctcgtggc ctgggggtcg
     2401 ccgtcgacga gcagcacccg acgccccgcg gtgctcagtg cgtgtgcgag atgcagcgcc
     2461 gtggaggact tgcccacccc acccttctgg ttcgtgatcg cccaaacgtt gtccatgtcg
     2521 gggaggctac cacatagctt gcttgctacc aagctttgtt gcacgaacga tcgcttgcca
     2581 cctttcaccg cagc
//
LOCUS       DASWGG010000315         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3180712, whole genome shotgun sequence.
ACCESSION   DASWGG010000315 DASWGG010000000
VERSION     DASWGG010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3180712"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VF954_05665"
     CDS             complement(<1..722)
                     /locus_tag="VF954_05665"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEX9530604.1"
                     /translation="MPRETFPGERRVALTPNALPALTKVGLRIIVESGAGAEAGFFDE
                     AYRHHGAEIGSRGDAFGADIVLQVRTYPANPDAGKPDLDLLRPDQLVIGLANPLANPE
                     GVAELARRKVTFFAMELIPRITRAQSMDALSSQANIGGYKAVIRAARLLPKMMPMMTT
                     AAGTIPPAKVFVLGVGVAGLQAIATARRLGAVVQAFDVRPETKEQVESLGAKFVEVPL
                     DTAEAKGTGGYAKEQSEEFLRR"
     gene            complement(846..>1512)
                     /locus_tag="VF954_05670"
     CDS             complement(846..>1512)
                     /locus_tag="VF954_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9530605.1"
                     /translation="WSHDSIGQGEDGPTHQPVEHLASLRAMPNLMLIRPADANETMEA
                     WRVALIHHDRPVGLVLTRQNVPVLDRTRLAPASGLAKGAYTLYDPPASNGGSPDPDLI
                     IIATGSEVSISVEAAERLAGEGIRARVVSMPSWDLFAAQPQSYRDEVLPPAVRGRLAV
                     EAAASFGWERWIGQDGACVCLDHFGASAPGARAMTEFGFTADNVAEHARRLVQRVASG
                     AKK"
BASE COUNT          200 a          533 c          554 g          225 t
ORIGIN      
        1 tggcggcgca ggaactcctc gctctgctcc ttggcgtagc cgcccgtgcc cttcgcctcg
       61 gccgtgtcga gggggacctc gacgaacttg gcgccgaggc tctccacctg ctccttggtc
      121 tccggccgga cgtcgaaggc ctggacgacg gcgccgaggc ggcgggcggt ggcgatcgcc
      181 tgcaggccgg cgaccccgac cccgagcacg aacaccttgg ccggcgggat cgtgccggcc
      241 gccgtggtca tcatcggcat catcttgggc aggagccggg cggcccggat caccgccttg
      301 tagccgccga tgttggcctg cgacgacagg gcgtccatgc tctgggccct ggtgatgcgt
      361 gggatcagct ccatggcgaa gaaggtcacc ttccgccggg ccagctcggc gacgccctcg
      421 gggttcgcga gagggttggc gaggccgatc acgagctggt cggggcggag gaggtccagg
      481 tcgggcttgc cggcatcggg attggccgga taggtgcgga cctgcagcac gatgtcggcc
      541 ccgaaggcat cgccccggga accgatctcc gccccgtggt gccgatacgc ctcgtcgaag
      601 aagccggcct cggcgccggc gccgctctcg acgatgatgc gcagcccgac cttcgtgagc
      661 gccgggaggg cgttcggggt cagggctacc cgacgctcac cggggaatgt ctcccgcggc
      721 actccgaccg acaatgaggc ctcatccgcc attcagccac ccctctccgc gcgcggccca
      781 ccggacgccc gggaggtcgc cgcatgccga cccccgccta ccccgatgag ctcatttctt
      841 cgccctcatt tcttggctcc cgacgcaact cgctgcacca gcctccgggc gtgctcggcg
      901 acgttgtccg ccgtgaaccc gaactcggtc atcgccctgg cccccggcgc cgaggcgccg
      961 aagtggtcga ggcagacgca ggcaccgtcc tggccgatcc agcgctccca gccgaagctg
     1021 gccgccgcct cgacggcaag gcggccccgg accgccggag gcaggacctc gtcccggtag
     1081 ctctggggct gggcggcgaa caggtcccac gagggcatgc tgacgacgcg ggcgcggatg
     1141 ccctcccccg cgaggcgctc ggcggcctcg acggagatcg agacctccga cccggtggcg
     1201 atgatgatga ggtcggggtc gggggatccg ccgttgctgg cgggcgggtc gtacagcgtg
     1261 taggcccctt tggcgagccc ggaggccggg gccagccggg tccggtcgag gaccggcacg
     1321 ttctggcggg tgaggacgag gccaaccgga cggtcgtgat ggatgagcgc gacccgccag
     1381 gcctccatgg tctcgttggc gtcggcgggc cggatgagca tgaggttggg catggccctg
     1441 aggctcgcca ggtgctcgac cggttggtgg gtcgggccgt cttcgccctg cccgatcgag
     1501 tcgtgcgacc ag
//
LOCUS       DASWGG010000316         3004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1570721, whole genome shotgun sequence.
ACCESSION   DASWGG010000316 DASWGG010000000
VERSION     DASWGG010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570721"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /gene="ahcY"
                     /locus_tag="VF954_05675"
     CDS             <1..1238
                     /gene="ahcY"
                     /locus_tag="VF954_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX9530606.1"
                     /translation="LGAELRWCSCNIFSTQDHAAAAVAVGPTGSVADPAGVPVYAWKG
                     ETLEDYWWCTEQVLRWPDGGGPNMILDDGGDATLLVHKGVEYEKAGSVPDPTGADNDE
                     WKVVLSTLRRSLADDANRWTTVASGIKGVTEETTTGVHRLYQMFEDGSLLFPAINVND
                     SVTKSKFDNLYGIRHSLIDGINRGTDVMLGGKVAVVCGYGEVGKGCAQALRGQGCRVI
                     VTEVDPICALQAVMEGFEVTTLERVVEQADIIVTATGNRGILLAGHMAKMKHNAIVGN
                     IGHFDNEIDMAGLARMSDVRHVSIKAQVDEFVFPDGHSVIVLSEGRLLNLGNATGHPS
                     FVMSMSFTNQVLAQIELFTRTSDYPLGVYVLPKQLDEKVARLHLSHLGVRLTELSQDQ
                     ADYLGVPRQGPYKHDHYRY"
     gene            complement(1256..2197)
                     /locus_tag="VF954_05680"
     CDS             complement(1256..2197)
                     /locus_tag="VF954_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX9530607.1"
                     /translation="MSARTRDLPRRSCLSIPGSSEKMLGKGPGLPADMVFLDLEDSVA
                     PLEKEPARAKAVDAIKNQDWGDKILCVRINSWDTKWTYADVIEVVGNGGERLDEVMLP
                     KVQSAAEVVALDLLLSQVEVNAGLDRGHIGIEAQIETTRGLINVEEICAASPRLEAII
                     FGPADFAASMQMPVLTGGVQIPEYPGDHFHYVFSKILMAGRANGLQVIDGPFLKIREL
                     DAFREYCNRTKILGYDGKWALHPDQVAILNEVFSPTPEQFERAWAILDAYQAATEGER
                     KGAVMFGDEMIDEASRKMATKFVSRGERAGLTRTPPE"
     gene            complement(2207..>3004)
                     /locus_tag="VF954_05685"
     CDS             complement(2207..>3004)
                     /locus_tag="VF954_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530608.1"
                     /translation="LRYVARADGAGPSLLVREAAGALAAFADDPAGLVTAARRLVDRH
                     PTAGPMWWLAARVLGAPDAMREAWLAAEELDDDPTASVLAGLLPSELTVVALGWPENV
                     AEALRRRGDLEALIVDACGEGGALARRLEASGVDAVDVPESGLAAAVAHAGLVLLEAT
                     AVGPDGFVATAGSRAAASVARSAGVPVWLVAGTGRVLPGRIWQALLRRLDLDDSEAWD
                     RIEEVVPLGLVDQVVGPSGPQPPGEAFRRADCPVAPELLKPVGGWSA"
BASE COUNT          461 a         1096 c         1031 g          416 t
ORIGIN      
        1 agctcggcgc cgagctgcgc tggtgcagct gcaacatctt ctcgacccag gaccacgccg
       61 ccgctgcggt cgcggtcggc ccgacgggca gcgtcgccga cccggccggc gttcccgtct
      121 acgcgtggaa gggcgagacc ctcgaggact actggtggtg caccgagcag gtcctgcgct
      181 ggcccgacgg cggaggcccc aacatgatcc tcgacgacgg cggggacgcc accctgctcg
      241 tgcacaaggg ggtcgagtac gagaaggccg gctcggtgcc ggacccgacc ggggccgaca
      301 acgacgagtg gaaggtcgtg ctctccaccc tgcgccgctc gctcgcggac gacgccaacc
      361 ggtggaccac tgtggcttcg gggatcaagg gggtcaccga ggagaccacg acgggcgtgc
      421 accggctgta ccagatgttc gaggacggga gcctgctctt cccggccatc aacgtgaacg
      481 actcggtgac gaagtcaaag ttcgacaacc tttacggcat ccggcactcg ctcatcgacg
      541 gcatcaaccg cggcaccgac gtgatgctgg gcggcaaggt cgccgtcgtc tgcggctacg
      601 gcgaggtcgg caagggctgc gcccaggccc tgcgggggca gggctgccgg gtgatcgtca
      661 ccgaggtcga ccccatctgc gccctccagg cggttatgga gggcttcgag gtcaccaccc
      721 tggagcgggt ggtggagcag gccgacatca tcgtcaccgc caccggcaac cgcggcatcc
      781 tcctcgccgg ccacatggcc aagatgaagc acaacgccat cgtcggcaac atcgggcatt
      841 tcgacaacga gatcgacatg gccgggctgg ccaggatgag cgatgtccgg cacgtgagca
      901 tcaaggccca ggtcgacgag ttcgtcttcc ccgacggcca ctcggtcatc gtcctgtcgg
      961 agggccgcct cctcaacctg ggcaacgcca ccggccaccc cagcttcgtg atgtcgatga
     1021 gcttcaccaa ccaggtgctg gcgcagatcg agctgttcac gcgcacctcg gactatcccc
     1081 tcggggtcta cgtcctgccc aagcagctcg acgagaaggt cgcccgcctc cacctgtccc
     1141 acctcggggt gcgcctcacc gagctgagcc aggaccaggc cgactacctg ggcgtgccga
     1201 ggcaggggcc gtacaagcac gaccactacc ggtactaggg ctcagctggc ggctgtcact
     1261 ctggcggggt gcgggtcagg ccggcgcgct ccccccggct gacgaacttg gtggccatct
     1321 tccggctggc ctcgtcgatc atctcgtcgc cgaacatcac ggcccccttg cgctccccct
     1381 cggtggccgc ctggtaggcg tcgaggatgg cccaggcccg ctcgaactgc tcgggggtgg
     1441 gcgagaacac ctcgttgagg atggccacct ggtccggatg gagggcccat ttgccgtcgt
     1501 agcccaggat cttggtgcgg ttgcagtact cgcggaaggc gtcgagctcg cggatcttca
     1561 ggaacggccc gtcgatcacc tgcagcccgt tggcccggcc ggccatgagg atcttggaga
     1621 acacgtagtg gaagtggtcg cccgggtact ccgggatctg gacaccgccg gtcagcacgg
     1681 gcatctgcat cgatgccgcg aagtcggccg gtccgaagat gatggcctcg agtcggggcg
     1741 acgccgcgca gatctcctcc acgttgatga ggccccgggt cgtctcgatc tgcgcctcga
     1801 tgccgatgtg gccccggtcc aggccggcgt tgacctcgac ctggctgagc agcaggtcga
     1861 gggccaccac ctcggccgcg gactggacct tcggcagcat gacctcgtcg aggcgttcgc
     1921 cgccgttgcc caccacctcg atcacgtcgg cgtaggtcca cttcgtgtcc cacgagttga
     1981 tccgcacgca gaggatcttg tcgccccagt cctggttctt gatggcgtcg accgccttgg
     2041 cccgggccgg ctccttctcc agcggggcca ccgagtcctc caggtcgagg aacaccatgt
     2101 cggcgggcag gcccggaccc tttcccagca tcttctcgct cgatccgggg atcgacaggc
     2161 aggagcgccg gggcaggtcc cgtgtgcgtg cggacattcc cgaagcctag gcggaccagc
     2221 cgcccaccgg cttgagcagc tccggcgcga ccgggcaatc ggcccgcctg aacgcctcgc
     2281 cgggcggctg gggcccggac ggccccacca cctggtcgac cagcccgagc ggcacgacct
     2341 cctcgatgcg gtcccaggcc tcgctgtcgt cgaggtccag ccgccgcagc agcgcctgcc
     2401 agatccgccc cggcaggacc cgcccagtcc cggcgacgag ccagacggga acgccggcgg
     2461 accgggccac cgacgccgcc gcccgcgagc cggctgtggc gacgaagccg tcggggccca
     2521 ccgccgtggc ctcgagcaac accaggccgg cgtgcgccac cgccgcagcc agcccagact
     2581 cgggcacgtc cacggcgtcg acccccgacg cctccagccg gcgggccaac gccccgccct
     2641 cgccgcaggc gtccacgatc agggcctcga ggtcgccgcg gcggcggaga gcctcggcga
     2701 cgttctcggg ccagccgagg gccacgacgg tcagctcgga gggtaggagg cccgccagca
     2761 ccgacgcggt gggatcgtcg tcgagctcct cggcggccag ccaggcctcc cgcatggcgt
     2821 caggggcacc cagcacccgg gcggccagcc accacatggg cccggccgtg ggatggcggt
     2881 ccaccagccg ccgggcggcc gtcaccaggc cggcgggatc gtcggcgaag gcggccaggg
     2941 cgccggcggc ctcgcgcacc agcagcgagg ggccggcgcc gtcggcccgg gccacgtacc
     3001 ggag
//
LOCUS       DASWGG010000317         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1573750, whole genome shotgun sequence.
ACCESSION   DASWGG010000317 DASWGG010000000
VERSION     DASWGG010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573750"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..953)
                     /locus_tag="VF954_05690"
     CDS             complement(12..953)
                     /locus_tag="VF954_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX9530609.1"
                     /translation="MSVGATIGLAVLVLVGIVFLVALRGMLNIVQQGQVGVVKRLGEY
                     RRTHEPGLVIIAPFIDSLQDVDMRETPVPGDRQEVITKDNVVVTVNATIFTQVVDAKQ
                     ALFSVKNFDIAIDALARTALRSVIGTMTLDEALSERERINTDVQQQMEVVTDKWGIRI
                     SRIEIVEIAPPPQILQALALQKTADQEKRAKILQSEGQQQSAINVAEGEAQAAVKAAD
                     GQRQSAVLRAEGARKAAILEAEGRAQAIETVYAAIKKAGPDPTLVAILQLDTLARFAD
                     SDNAKIVVPYESAALLGAVQALKGVMDGVSADGAAKK"
     gene            complement(950..1504)
                     /locus_tag="VF954_05695"
     CDS             complement(950..1504)
                     /locus_tag="VF954_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX9530610.1"
                     /translation="MVLAWLVIAAVLLLFELHHLAFYALFGTVGCLAAAAVALVAPDA
                     IAAQGAVAVGVALAGVVTVRPLVSKAFARHRGGGVARGVHGGLAGQEAITLDEVADAH
                     VVGHVRLAGERWLAMTGDGRRIGPGTRVVVAAVQGTTLVVWPTDQGAGELAETGAPDR
                     EGHREDDREDDREATPDGADGRAR"
     gene            complement(1564..2274)
                     /locus_tag="VF954_05700"
     CDS             complement(1564..2274)
                     /locus_tag="VF954_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX9530611.1"
                     /translation="MPAVACPNGHESATSDYCDQCGARIEGAGPPPPAAPTPEPAAGA
                     APPPAPPPATGAAEDLECPNCRSPRSTEEKFCEVCGYDFVLGTLPAAPKVEAAVAPSG
                     PPWEAVVSADRDYFERNDVDDVTFPDNCPDRVFTLSEGELLIGRRSERRGVRPQIDLS
                     GAPEDPGVSHAHALLRRQADGSYAVVDPGSTNGTTLNEGTAPLPQNVVTPLKDGDTLY
                     LGAWTRILFHAPAPHPPP"
     gene            complement(2274..>2688)
                     /locus_tag="VF954_05705"
     CDS             complement(2274..>2688)
                     /locus_tag="VF954_05705"
                     /inference="COORDINATES: protein motif:HMM:NF036903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530612.1"
                     /translation="KPAAVGAEMLAGRVSLVVNDEVASQALVKATWTDDAALSTKINN
                     AVAHYTGQAELAQVIQEGLEARKAGDTATATTKLGRAVQLAAESGHDGTTRLLAKVVE
                     VEDANTGKVRLKSGVDDADEMALDTRSTKTTRVKH"
BASE COUNT          348 a          968 c          935 g          437 t
ORIGIN      
        1 cccagggtca gctacttctt tgccgctccg tccgcggaca ccccgtccat cacacccttg
       61 agggcctgta ccgcgcccag gagcgccgcg ctctcatagg gaacgacgat cttggcgttg
      121 tcgctgtcgg cgaaccgggc cagggtgtcg agttggagga tggccacgag cgtggggtcg
      181 ggcccggcct tcttgatggc ggcgtacacc gtctcgatgg cctgagcccg cccttcggct
      241 tccaggatgg ctgccttgcg agctccctcg gcgcgcagga cggcggactg gcgctggccg
      301 tcggccgctt tcaccgcggc ctgggcctcc ccctcggcca cgttgatggc cgactgctgc
      361 tggccctcgg actggagaat cttcgcccgc ttctcctggt cggccgtctt ctggagcgcc
      421 agggcctgga ggatctgggg cgggggggcg atctcgacga tctcgatccg gctgatccgg
      481 atgccccact tgtcggtgac cacctccatc tgctgctgga cgtcggtgtt gatgcgctcc
      541 ctctcggaga gcgcctcgtc gagggtcatg gtgccgatca cggagcgcag ggccgtgcgg
      601 gccagggcgt cgatggcgat gtcgaagttc ttcacgctga acagcgcctg cttggcgtcg
      661 accacctggg tgaagatcgt ggcgttgacg gtgacgacca cgttgtcctt cgtgatcacc
      721 tcctggcggt cgccgggcac cggcgtctcc cgcatgtcga cgtcctggag cgagtcgatg
      781 aacggggcga tgatcaccag gccgggctcg tgcgtgcggc ggtactcgcc caggcgcttg
      841 accacgccca cctggccctg ctggacgatg ttgagcatgc ctctgagagc caccaggaac
      901 acgatgccga ccaggaccag gacggccaat ccaatggtgg cgccgacgct catcgcgctc
      961 tcccgtccgc cccgtcgggc gtcgcttccc ggtcgtcttc ccggtcgtct tctcggtggc
     1021 cttcccggtc gggtgccccc gtctccgcca gctcgcccgc gccctggtcg gtgggccaca
     1081 ccacgagcgt ggtcccctgg accgcggcca ccacgaccct cgtgcccggg ccgatgcggc
     1141 ggccgtcgcc ggtcatcgcc agccaccgct cccccgccag ccgcacgtgg ccgacgacat
     1201 gggcgtcggc cacctcgtcg agggtgatcg cctcctgccc ggccagtccg ccgtgcaccc
     1261 cgcgcgccac cccgccgccg cggtgcctgg cgaaggcctt gctcacgagg gggcgcacgg
     1321 tcaccacgcc ggccagcgcc acgccgaccg cgacggcccc ctgcgcggcg atggcgtcgg
     1381 gggccaccag ggcgacggcc gccgcggcca ggcacccgac ggtgccgaac agcgcgtaga
     1441 aggcgaggtg gtgcagctcg aagaggagga gcacagccgc gatcaccagc caggccaaga
     1501 ccatggcttt cacctccttg ccaagagggt agctcgcagc gaccggccgg cctcgggctc
     1561 gccctagggc ggtgggtggg gcgccggcgc gtggaagagg atcctggtcc acgcgccgag
     1621 gtacagcgtg tccccgtcct tgagaggcgt caccacgttc tgcggcagcg gcgcagtccc
     1681 ctcattgagc gtcgtgccat tggtggatcc cgggtccacc accgcgtagg acccatcggc
     1741 ttgccggcgg agcaaggcat gggcgtgtga gacacccggg tcctcgggcg ctccgctcag
     1801 gtcgatctgg ggccggaccc cgcgacgctc gctgcgacga ccgatcaaca gctcaccctc
     1861 gctcagcgtg aagacgcgat ccggacagtt gtcggggaag gtcacgtcgt cgacatcgtt
     1921 gcgttcgaag tagtcacggt ccgcgctcac gaccgcctcc cacgggggtc ccgacggtgc
     1981 gaccgcagcc tccaccttcg gagcggccgg caaggtgccc aggacgaagt cgtacccgca
     2041 cacctcgcag aacttctcct cggtcgaccg cgggctccgg cagttggggc actcgagatc
     2101 ctcggcagca cccgtggcag gcggaggcgc gggcggcgga gcagcccccg cggcgggctc
     2161 gggcgtgggc gcggcgggag ggggcggccc agcaccctcg atgcgggctc cgcattggtc
     2221 gcagtagtcg ctcgtcgccg actcatggcc attggggcac gcgacggcgg gcatcagtgc
     2281 ttgacgcgcg tggtcttggt agagcgcgtg tcgagggcca tctcgtcggc gtcgtcgacg
     2341 ccgctcttga gccggacctt gcccgtgttg gcgtcctcga cctcgaccac cttggccagc
     2401 agacgcgtgg tgccgtcgtg gccggactcg gccgccagct ggacggcgcg ccccagcttg
     2461 gtcgtcgccg tcgcggtgtc gcccgccttg cgcgcctcca gcccctcctg gatgacctgg
     2521 gccagctcgg cctgcccggt gtaatgagcg accgcgttgt tgatcttggt ggacagcgcc
     2581 gcgtcgtcgg tccaggtggc tttcaccagg gcctgggacg caacttcgtc attgaccacg
     2641 aggctgacgc ggccggccag catctcggca cccacggctg ccggcttg
//
LOCUS       DASWGG010000318         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1574583, whole genome shotgun sequence.
ACCESSION   DASWGG010000318 DASWGG010000000
VERSION     DASWGG010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574583"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VF954_05710"
     CDS             <1..511
                     /locus_tag="VF954_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530613.1"
                     /translation="GHGAHVHSAGLLEGGRAVTDDGLSVMAARGLDTSGHLSRCISPA
                     MLSAADLILGMTRDHVREAVLLVPLVWPRTFTLKELVRRGRAVGPRPGGMALRGWLDL
                     VGRGRERGDLLGDSPADDVADPVGQGRDAYQRTADELDGLTRQLAALVWARCAPASAT
                     TVRGVIPCP"
     gene            502..>1707
                     /gene="glyA"
                     /locus_tag="VF954_05715"
     CDS             502..>1707
                     /gene="glyA"
                     /locus_tag="VF954_05715"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX9530614.1"
                     /translation="MPLGEPTDPELFALVDRELERQSTTFQLIASENFTSRAVMAATG
                     SVLTNKYSEGYPGRRYYGGNHVIDEVEDLARERAKALFGAQHANVQPHSGANANLAAY
                     LAVLDAGDTVLGMRLDQGGHLTHGSPVNVSGRFYRFVSYGVTDSDERIDLDQLAELAR
                     AERPKLIVAGATAYPRVIDPEPFRRIADEVGALFLFDAAHVAGLIAGGVHPNPVPFAD
                     IVTLTTHKTLRGPRGGCVLSTAAHAAAVDKAVFPGLQGGPLEHVVAAKAVAFHEAAQP
                     DFAEYARRIVVNARALAAGLAAEGFRLVSGGTDNHLLLVDLRAFDEDLTGKVAQEVLD
                     RAGITTNKNTVPNDPRSPFVTSGLRLGTAATTTTGMGPDEMGEIARLIGHTLRHRDSG
                     AELASVKDEV"
BASE COUNT          237 a          658 c          595 g          217 t
ORIGIN      
        1 tgggcacggc gcccacgtcc actcggccgg gctcctcgag gggggccggg cggtgaccga
       61 cgacggcctg agcgtcatgg cggcccgggg cctggacacc tccggccatc tcagccggtg
      121 catcagcccg gccatgctca gcgccgcgga cctgatcctc ggcatgaccc gggaccacgt
      181 gcgggaggcc gtgctgctcg tgcccttggt ctggccccgg accttcaccc tgaaggagct
      241 cgtccggcgg ggccgcgccg tcggtccccg cccgggcggg atggcgcttc ggggctggct
      301 ggacctggtc ggccggggcc gagagcgggg cgacctgctc ggcgactccc cggccgacga
      361 cgtggccgac cccgtcgggc agggccggga cgcgtaccag cgcactgccg acgagctgga
      421 cgggctcacc cggcagctgg cggccctggt ctgggcccgg tgtgcccccg cctccgctac
      481 caccgttcga ggagtgatcc catgccccta ggggagccga ccgaccccga gctgttcgcc
      541 ctcgtcgacc gggagctcga gcgccagagc acgacgttcc agctgatcgc ttcggagaac
      601 ttcacctcgc gcgccgtgat ggccgcgacg ggctcggtgc tgaccaacaa gtacagcgag
      661 gggtacccgg gcaggcgcta ctacggcggc aaccacgtca tcgacgaggt cgaggacctg
      721 gcccgagagc gagccaaggc cctgttcggc gcccagcacg ccaacgtcca gccccactcg
      781 ggggccaacg ccaacctggc cgcctacctg gcggtgctgg acgcgggcga cacggtgctg
      841 ggcatgcgcc tcgaccaggg cggccacctg acccacggct cgccggtcaa cgtcagcggc
      901 cgcttctacc ggttcgtctc ctacggggtg accgacagcg acgagcgcat cgacctcgac
      961 cagctcgccg agctggcccg ggccgagcgg ccgaagctga tcgtcgccgg cgccaccgcg
     1021 tacccccgcg tcatcgaccc cgagcccttc cggaggatcg ccgacgaggt cggggcgctc
     1081 ttcctcttcg atgccgccca cgtggcgggc ctgatcgccg gaggggtgca tcccaacccg
     1141 gtgccgttcg cggacatcgt gacgttgacc acccacaaga cgctgcgcgg cccgcgcggc
     1201 ggttgcgtcc tgtccaccgc cgcccacgcc gcggccgtcg acaaggccgt gttccccggc
     1261 ctgcagggcg gccccctaga gcacgtcgtg gcggccaagg cggtcgcctt ccacgaagcg
     1321 gcccagcccg acttcgccga gtacgcccgg cggatcgtgg tcaacgcccg ggccctggcg
     1381 gccggcctgg ccgccgaggg cttccggctc gtctcgggcg ggaccgacaa ccaccttctc
     1441 ctcgtcgacc tccgcgcctt cgacgaggac ctcaccggga aggtcgccca ggaggtgctc
     1501 gaccgggcgg ggatcacgac caacaagaac acggtgccca acgatccccg gtccccgttc
     1561 gtcaccagcg ggttgcggct cggcaccgcg gccaccacga ccacgggcat gggccccgac
     1621 gagatgggcg agatcgcccg cctgatcggc cacaccctgc gccaccggga cagcggcgcc
     1681 gagctggcct cggtgaagga cgaggtc
//
LOCUS       DASWGG010000319         1829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2070598, whole genome shotgun sequence.
ACCESSION   DASWGG010000319 DASWGG010000000
VERSION     DASWGG010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2070598"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(426..1673)
                     /locus_tag="VF954_05720"
     CDS             complement(426..1673)
                     /locus_tag="VF954_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9530615.1"
                     /translation="MTRPFDEIDASTLAFWELSAEERDARLAVLREKRPVSWQRPAEG
                     MATLPGRDDPGYWAVVRHADVMTVSRDPETYCSSKGFQIEDIPEEILEAAGSFLALDH
                     PRHTKLRRLVSVAFSPRRVRALEDKIAARAATLVDDLLEQGEGDFVEVVSTPMPSATF
                     AEMMGVGEEDRERVTGLANDMVSWNDPDVLDGRDGLTLLLETLVGLYGEAAALSDARR
                     AQPADDILTGLVTAEVDGERLTDEEIAAFFVLLSVAANDTTRQSISHGMRALCRFPDQ
                     RALLASDLDRHLPGAVEEMVRWATPVMTFRRTATRDAELGGMPIAVGDKVVMLYVSAN
                     RDESVFAGPDRFDIRRHPNPHLVFGHGPHFCLGAHLARVQMRALFAEVLARTSALAPA
                     GPPSYLRSNFQRGVKRLPVAWTA"
BASE COUNT          280 a          607 c          648 g          294 t
ORIGIN      
        1 gccgtgccgc gccgtccttc ggttcgtacg tgaccaaggc ctcttcccat caccgattct
       61 gccccagtcc aacttagcgg taccccctga catttccgga gatgattagg agcctgggct
      121 ggacggccgt tacggccctg acgagtggga aaggccaagg gcgggccatc gatgagcgaa
      181 tcggggaacc cagaacgccg ctgaagccgt cgggccgccg cggcctcagc tggacgtctc
      241 acttccccca ttgtcctcat caacagctag aatcgctcac ctgtacgagt gatcttaact
      301 tttgcgtgga ggattcgggc ttcgtggagg atttagggta ctggagtgca ctggatcctc
      361 cacggaaccc ttttcttcca tgatcttttg cggcggaacg caccgtggcg ccggagaacg
      421 gctggctagg cggtccaggc gacggggagg cgcttgacgc cgcgctggaa gttggaccgc
      481 aggtaggacg gcgggccggc cggggcgagg gcggacgtgc gggccagcac ctcggcgaag
      541 agcgcgcgca tctggacccg ggccaggtgc gcgcccaggc agaagtgcgg gccgtggccg
      601 aacaccaggt gcgggttggg gtggcggcgg atgtcgaagc ggtccgggcc ggcgaagacg
      661 gactcgtcgc ggttggccga gacgtagagc atcaccacct tgtcgccgac ggcgatcggc
      721 atgccgccga gctcggcgtc gcgggtggcg gtgcggcgga aggtcatcac cggggtggcc
      781 cagcggacca tctcctccac ggcgccgggc aggtggcggt cgaggtcgga ggccagcagg
      841 gcccgctggt cggggaaccg gcacagggcc cgcatgccgt ggctgatcga ctggcgggtc
      901 gtgtcgttgg ccgccaccga caacagcacg aagaaggcgg cgatctcctc gtcggtcagg
      961 cgctcgccgt cgacctcggc cgtcacgagg ccggtcagga tgtcgtcggc cggctgggcc
     1021 cgccgggcgt cggacagcgc cgccgcttcg ccgtagaggc cgaccagcgt ctcgagcagc
     1081 agcgtgaggc cgtcgcgccc gtcgaggacg tcgggatcgt tccacgacac catgtcgttg
     1141 gccaggccgg tgacccgctc ccggtcctcc tcgccgacgc ccatcatctc ggcgaaggtg
     1201 gccgacggca tcggcgtcga caccacctcg acgaagtcgc cctcgccctg ttccagcaga
     1261 tcgtcgacca gggtcgccgc ccgcgccgcg atcttgtcct ccagggcccg cacccgccgg
     1321 ggcgagaagg ccaccgagac cagccggcgg agcttggtgt gccgggggtg atcgagggcc
     1381 aggaaggatc ccgccgcttc caggatctcc tcggggatgt cctcgatctg gaaccccttg
     1441 ctggagcagt acgtctccgg atcacggctg accgtcatca cgtcggcgtg gcggacgacc
     1501 gcccagtagc cgggatcgtc ccgaccgggc agcgtcgcca tgccctcggc cggtcgctgc
     1561 cacgacaccg gtcgcttttc ccggaggacc gccagtcggg cgtcccgctc ctcggcgctg
     1621 agctcccaga aggccagggt cgacgcgtcg atctcgtcga agggtcgggt catcaccata
     1681 tgatgacata tgttatgcca cggcggtagg gccaacgctt acacgccgtc tcgcgggcta
     1741 cgaagcaacg tcttgtcggc gttcgggttc gggtcccaca tcgaggcgta cgacatggcg
     1801 ccgttgtacc cagggccgtt gtcgtggtg
//
LOCUS       DASWGG010000320         6309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2071031, whole genome shotgun sequence.
ACCESSION   DASWGG010000320 DASWGG010000000
VERSION     DASWGG010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2071031"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VF954_05725"
     CDS             complement(<1..322)
                     /locus_tag="VF954_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530616.1"
                     /translation="MDRDALDRDVMDADLLLDPPSALPDPVRRLARRAGTDRGLRRLW
                     AGVAMLFLLGLASCMRAGANHPADPYLSNERVPSGVGSFAKVGFRIVHVVDGAEARGP
                     EHCAL"
     gene            complement(343..1224)
                     /gene="panB"
                     /locus_tag="VF954_05730"
     CDS             complement(343..1224)
                     /gene="panB"
                     /locus_tag="VF954_05730"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX9530617.1"
                     /translation="MAQQITVPAVRARKAAAGAPPLVMVTAYDAPGARMADEAGVDLI
                     LVGDSVAMVVLGYDDTLQVTIDDLVHHTAAVARAKPRPLVVADLPWLSYHLDAADTVR
                     NAARLIRAGAAAVKLEGGTKRTPMVGALLDAEIPVMGHLGLTPQSVHAMGGFKVQGKE
                     RAAAEALVADAVALAEAGCFSIVLECVPDAVARLVTDAVAVPTIGIGAGPSCDGQVLV
                     LHDVLGLEDRIAPKFVRRYAQLKADGVAALAAYAADVRAGRFPSDAESYHLSAASSAE
                     LASIAGLYGSGGITQSA"
     gene            1305..1493
                     /locus_tag="VF954_05735"
     CDS             1305..1493
                     /locus_tag="VF954_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530618.1"
                     /translation="MKATCESCGGLDDDLVAVHRLYVVPEAWDTPGSVTRVEEIERWC
                     FPCRSMYPHEVAGERAGS"
     gene            complement(1499..3601)
                     /locus_tag="VF954_05740"
     CDS             complement(1499..3601)
                     /locus_tag="VF954_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX9530619.1"
                     /translation="MRPKSWLWRHRKPLFLLTLVATGGLAAAAYAVLAIPLPPAHVEA
                     QTSMLFDANNHLLASLSGTENRVNQPLSAVPKVVRDAVVATEDHGYYHHGGVDPIGLV
                     RAAVTDVRSGRGAQGGSTITQQYVKNVYVGNRRTLFRKLREAMLAIKVERTSTKQQIL
                     ERYLNTIYFGRGAYGVGAASQAYFGRPVSALGLPEAAYLAGLIRAPVVADYSNAPAAA
                     TRRRNLSLQSMERYRFITPAARAGAEAVPLATYVIAPVAQSDAALAPGEPAGSEYFVQ
                     YVRAQLVRRFGETAVSAGGLRVRTTLDPALQQHAYDAVYGPGGLRPGEPAGALVATDD
                     GGHIRAMVGGRDYQVSKVNLALGQAGGGSGRQAGSTFKPFLLAEAVNEGYTVQSQFDA
                     PDKLVIPKADNGKDYTVGNFGGESFPGQINLIDATKSSVNTVYAQLQYLIGPRHLVDM
                     AHALGVQSTLEPNVSLVLGTSDVSVLEMASAYSTLANQGLSYFSQSSAYDLDPHSILE
                     VGTADGVALDKPRITPVRVLDARKAAVVTYCLQQVVSSGTGQGARIGRPLAGKTGTTE
                     DFGDAWFIGYTPRLTAALWMGYPQGSDHKMTDVRGIPVQGGGIPAELFRRFMAAALKD
                     EPAYGTGQFPVVTSFPGRSLSSSQLPFTVSPATTTTTSTTTTTLPGGTTTTSTSVKPG
                     ATTTTASAATTTTTAKPG"
     gene            complement(3611..4027)
                     /locus_tag="VF954_05745"
     CDS             complement(3611..4027)
                     /locus_tag="VF954_05745"
                     /inference="COORDINATES: protein motif:HMM:NF036456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HEX9530620.1"
                     /translation="MSFDPQSVRGGSPLGDLPGQIDYRLARNSVISEFRKGRLSRLDV
                     CDAQAELLRAAQNVGEPTGETCPICEEAALVHVSYAFGPRLPPSGTCVSSRRELVRLT
                     RSTAQVACYVVEVCPDCRWNHLAKTFVVGGQRARTS"
     gene            4198..4824
                     /locus_tag="VF954_05750"
     CDS             4198..4824
                     /locus_tag="VF954_05750"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9530621.1"
                     /translation="MLELAILGVLKETELHGYELKKRLADALGPFSSVSFGSLYPALA
                     RLEAAGAVKAVEAFAPAGGPATPMTGSLSGELAAFRARRASTRGQRGKKVYGITERGE
                     QLFEELLAAGSGPEDERAFNLRLAFARYLPDDERLGMLERRRAQLVERLAKARAALRA
                     GRERLDGYGRSLIDHSTESTERDISWLDRLIAAERTPKDGKPKHLEDN"
     gene            4826..5905
                     /locus_tag="VF954_05755"
     CDS             4826..5905
                     /locus_tag="VF954_05755"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX9530622.1"
                     /translation="MTKIRVAIAGVGNCASSLVQGVEHYRNADPAESVPGLMHVSLGG
                     YHVGDVEFVAAFDVDAAKVGLDLGKAIHSGQNNTIRFANVGYLGVTVQRGPTFDGLGK
                     YYRQTIEESPFEPVDVARVLEDTKADVLVSYLPVGSEAAQKHYAQAAIDARVGFVNAI
                     PVFIASDPVWAAKFTEAGVPIVGDDIKSQVGATIVHRILARLFEDRGMVLDRTYQLNV
                     GGNMDFKNMLERERLESKKISKTQAVTSQIDNGIAADDVHIGPSDHVPWLEDRKWAYI
                     RMEGRNFGDVPLNVELKLEVWDSPNSAGVIIDAVRAAKIARDRGIGGPVLSAASYFMK
                     SPPEQYGDDVARDLVEKFIHGECER"
     gene            5962..>6309
                     /locus_tag="VF954_05760"
     CDS             5962..>6309
                     /locus_tag="VF954_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9530623.1"
                     /translation="MTVLAGFRERLRGRGTGSGSSFLGDLKTVLRERNFRRLLATRLI
                     SQGGDGAFTAGVGAYVFFSKATFPNPAAAAAAFAVLYLPYSLVGPFAGVFIDRWSRRQ
                     ILVWSALIRAAFVV"
BASE COUNT          908 a         2186 c         2323 g          892 t
ORIGIN      
        1 ggagcgcaca gtgctcggga ccccgagcct ccgcgccgtc cacgacgtgg acgatgcgaa
       61 agccgacctt ggcgaacgac ccgacccctg acgggacccg ctcgttgctg agatagggat
      121 cggccgggtg gttggccccg gcccgcatgc agctggccaa cccgagcagg aacagcatcg
      181 ccaccccggc ccacagccgc ctcagccccc ggtcggtccc ggcccggcgg gccaggcgac
      241 gcacagggtc ggggagcgcc gaggggggat cgagcagcag gtcggcgtcc atgacatctc
      301 ggtccagggc gtctcggtcc acgccgccat gttggcgcgg gctcaggccg actgcgtgat
      361 cccccccgag ccgtagagcc cggctatgga ggccagctcg gccgagctgg ccgccgacag
      421 gtggtaggac tcggcgtcgc tggggaagcg cccggcccgc acgtcggcgg cgtaggcggc
      481 cagggcggcc accccgtcgg ccttgagctg ggcgtagcgg cgcacgaact tcggcgcgat
      541 ccggtcctcc agccccagca cgtcgtggag caccagcacc tggccgtcgc acgagggccc
      601 ggcgccgatg ccgatggtgg gcaccgccac cgcgtcggtc acgaggcggg cgaccgcgtc
      661 gggcacgcat tcgaggacga tggagaagca cccggcctcg gccagggcca cggcgtcggc
      721 gaccagggcc tcggccgcgg cgcgctcctt gccctgcacc ttgaagccac ccatggcgtg
      781 caccgactgc ggggtcagcc cgaggtggcc catgacgggg atctcggcgt cgagcagggc
      841 gccgaccatc ggggtgcgct tggtgccgcc ctccagcttg accgcggccg cgccggcccg
      901 gatcagccgg gccgcatttc tcaccgtgtc ggcggcgtcc aggtggtacg acaaccatgg
      961 caggtcggcc acgaccagcg gccggggctt ggcccgcgcc accgccgcag tgtggtgcac
     1021 gaggtcgtcg atggtgacct ggagggtgtc gtcgtagccc agcacgacca tggcgacgga
     1081 atcgccgacg aggatgaggt ccacccccgc ctcgtccgcc atacgggcgc cgggggcgtc
     1141 gtaggcggtg accatcacga gcggtggcgc gccggcggcc gccttgcggg cgcgcacggc
     1201 cgggaccgtg atctgctgag ccatgattgg ccctcctttt caacccgtcc agcgccccac
     1261 ggctgccggc ggtgctcgcg agtgtaggcg gtatcgtcgg gcgcgtgaaa gccacgtgtg
     1321 agagctgcgg cgggctcgac gacgacctcg tggccgtcca ccggctctac gtcgtgcccg
     1381 aggcctggga cacccccgga tcggtcaccc gggtcgagga gatcgagcgc tggtgcttcc
     1441 cctgccggtc catgtacccc cacgaggtgg ccggggagag ggccggctcc tagggcggct
     1501 accccggctt ggcggtcgtc gtggtggtcg ccgccgaggc ggtggtggtg gtggccccgg
     1561 gcttcacgct ggtcgacgtg gtggtggtcc cgcccggaag cgtcgtggtg gtcgtgctgg
     1621 tggtcgtggt ggtcgccggc gacaccgtga agggcagctg ggacgacgag agggaacggc
     1681 cggggaagct ggtcaccacc gggaactggc cggtgccgta cgccggctcg tccttcaggg
     1741 cggcggccat gaaccggcgg aacagctcgg ccgggatgcc gcccccctgc accgggatcc
     1801 cccgcacgtc ggtcatcttg tggtcgctgc cctgggggta gcccatccag agagccgccg
     1861 tcagccgtgg tgtgtacccg atgaaccagg cgtcgccgaa gtcctcggtc gtgcccgtct
     1921 tgccggccag cgggcggccg atccgggcgc cctggccggt gccggaggac acgacctgct
     1981 ggaggcagta ggtcaccacg gcggccttgc gggcgtcgag gacccggacc ggggtgatcc
     2041 tcggcttgtc cagcgccacg ccgtcggccg tgcccacctc caggatgctg tgcgggtcca
     2101 ggtcgtaggc cgaggactgg gagaagtacg acagcccctg gttggccagc gtcgagtagg
     2161 cggacgccat ctccagcacg gacacgtccg aggtgccgag gaccagggac acgttgggct
     2221 cgagggtgga ctgcacgccc agggcatggg ccatgtccac caggtggcgc ggaccgatca
     2281 ggtactggag ctgggcgtag acggtgttga ccgagctctt ggtggcgtcg atgaggttga
     2341 tctgcccggg gaaggactcg cccccgaagt tgcccaccgt gtagtccttg ccgttgtcgg
     2401 ccttggggat gaccagcttg tcgggcgcgt cgaactgcga ctgcaccgtg tagccctcgt
     2461 tcaccgcctc ggccagcagg aagggcttga aggtcgaccc ggcctggcgc cccgagcccc
     2521 cgccggcctg gcccagggcc aggttgacct tggagacctg gtagtcgcgg cccccgacca
     2581 tggcccggat gtggccgccg tcgtcggtgg ccaccagggc gccggcgggc tctccgggtc
     2641 gcagcccgcc ggggccgtag acggcgtcgt aggcgtgctg ctggagagcc gggtcgagcg
     2701 tggtcctgac ccgcagcccg cccgccgaga ccgccgtctc cccgaagcgg cgcaccagct
     2761 gggcgcgcac gtactgcacg aagtactccg agccggcggg ctcccccggg gccagggcgg
     2821 cgtcgctctg ggccaccggc gcgatcacgt aggtggcgag gggcacggcc tcggctcccg
     2881 cgcgcgccgc aggcgttatg aaccggtagc gctccatcga ctggaggctc aggttgcgcc
     2941 gccgggtggc tgccgccggc gcgttcgagt agtcggccac gaccggagcc cggatcaggc
     3001 cggccaggta ggcggcctcg ggcaggccga gggcgctgac cggtcggccg aagtaggcct
     3061 gcgacgccgc cccgaccccg taggcgcccc gcccgaagta gatggtgttg aggtagcgct
     3121 cgaggatctg ctgcttggtc gaggtgcgtt cgaccttgat ggccagcatc gcctcccgca
     3181 gcttccggaa cagcgtgcgc ctgttgccga cgtagacatt cttcacgtac tgctgggtga
     3241 tggtggagcc gccctgggcg ccccggccgc tgcgcacgtc ggtgaccgcg gcgcggacca
     3301 gcccgatcgg gtccacgccc ccgtggtggt agtagccgtg gtcctcggtg gcgaccacgg
     3361 cgtcgcgcac gaccttgggc acggccgaga ggggctggtt gacgcggttc tcggtgccgg
     3421 agagcgacgc cagcaggtgg ttgttggcgt cgaacagcat cgaggtctgg gcctcgacgt
     3481 gcgcgggagg cagggggatg gccagcacgg cgtaggccgc ggccgccagc ccacccgtcg
     3541 cgaccagggt caggagaaag agcggcttgc ggtggcgcca cagccagctc ttgggtcgca
     3601 ccggtgggcg tcagctggtg cgggcccgtt ggccaccgac gacgaaggtc ttggccagat
     3661 ggttccaacg gcagtccggg cacacctcga cgacgtagca cgccacctgg gccgtggacc
     3721 gggtcaggcg caccagctcc cggcggctgc tgacgcaggt cccgctgggc ggtaggcggg
     3781 ggccgaaggc gtaggagacg tgcacgagcg cggcctcctc gcagatcggg cacgtctcgc
     3841 cggtgggctc gccgacgttc tgggcggccc gcagcagctc ggcctgggcg tcgcagacgt
     3901 cgaggcgcga caggcggccc tttcggaact cgctgatgac ggagttgcga gccaggcggt
     3961 agtcgatctg gccgggcagg tcgcccaggg ggctcccgcc gcggaccgac tggggatcga
     4021 agctcacggc gtcagggtag cccgggcccg cccgcggttc tcgggaccgg gcggggctcg
     4081 gcgacgggcc agagcgacca gttggttgac aacaggcgaa caacgggtag agtaggaatc
     4141 tcccgatata tcgatccgat acatcgtcag tctcaagcga cggaccgaaa ggcagacgtg
     4201 ctggaactcg ccatcctcgg cgtgctcaag gagaccgagc tgcacggcta cgagctcaag
     4261 aagcgcctgg ccgacgcgct cgggcccttc tcgagcgtgt cgttcggctc cctctacccg
     4321 gcgctcgccc gcctcgaggc cgccggggcg gtcaaggcgg tcgaggcctt cgcccccgcc
     4381 ggcgggccgg ccacgccgat gacggggtcc ctgagcgggg agctggccgc cttccgggcc
     4441 cggcgggcgt ccacccgggg ccagcggggc aagaaggtgt acgggatcac cgagcggggc
     4501 gagcagctct tcgaggagct gctggctgcg gggagcgggc ccgaggacga gcgggcgttc
     4561 aacctgcggc tggccttcgc ccgctacctg cccgacgacg agcgcctcgg catgctcgag
     4621 cgccggcggg cccagctggt cgagcgactg gccaaggccc gggccgccct gcgcgccggc
     4681 cgggagcgcc tggacggcta cggccggtcg ctcatcgacc acagcaccga gagcaccgaa
     4741 cgcgacatct cctggctcga ccgcctcatc gccgccgagc ggacacccaa ggacggcaag
     4801 cccaagcacc tggaggacaa ctgacatgac caagatccgc gtcgccatcg ccggggtggg
     4861 caactgcgcc agctcgctgg tgcagggcgt cgagcactac cgcaacgccg atccggccga
     4921 gagcgtcccg ggcctgatgc acgtcagcct cggcggctac cacgtcgggg acgtggagtt
     4981 cgtggccgcc ttcgacgtcg acgcggccaa ggtcggcctc gacctcggca aggccatcca
     5041 ctccgggcag aacaacacca tccgcttcgc caacgtcggt tacctcggcg tgaccgtgca
     5101 gcggggcccc accttcgacg gcctgggcaa gtactaccgc cagaccatcg aggagtcccc
     5161 gttcgagccg gtcgatgtcg cccgcgtgct cgaggacacc aaggccgacg tcctggtcag
     5221 ctaccttccc gtcggctccg aggcggccca gaagcactac gcccaggcgg ccatcgacgc
     5281 ccgggtcggg ttcgtcaacg ccatcccggt gttcatcgcc agcgacccgg tgtgggccgc
     5341 caagttcacc gaggccggcg tgcccatcgt gggcgacgac atcaagagcc aggtgggcgc
     5401 gaccatcgtg caccgcatcc tggcccgcct gttcgaggac cgggggatgg tgctggaccg
     5461 gacctaccag ctcaacgtcg gcggcaacat ggacttcaag aacatgctcg agcgcgagcg
     5521 gctcgagtcc aagaagatct ccaagaccca ggccgtcacc agccagatcg acaacggcat
     5581 cgcggccgac gacgtccaca tcggcccgtc ggaccacgtg ccgtggctcg aggaccgtaa
     5641 gtgggcctac atccgcatgg agggtcgcaa cttcggcgat gtgcccctca acgtcgagct
     5701 caagctggag gtgtgggact cgcccaactc cgccggcgtg atcatcgatg ccgtccgcgc
     5761 cgccaagatc gcgcgcgacc gcgggatcgg cggcccggtc ctgtccgccg cgtcgtactt
     5821 catgaagtcc ccgcccgagc agtacggtga cgatgtcgcc agggacctgg tggagaagtt
     5881 catccacggc gagtgcgagc gctgatccag agctggagcg cggggcagct tccgggagcc
     5941 ggacggcaat aggctgagcg cgtgacggtg ctggctggct tccgggaaag gctgcgcggc
     6001 cggggcacgg gttccggctc gtcgttcctc ggcgacctga aaaccgtgct gcgggaacgg
     6061 aatttccgcc gcctgctcgc gacccggctg atctcccagg gcggggacgg ggcattcacc
     6121 gccggagtcg gcgcgtacgt tttcttcagc aaggcgacct ttcccaaccc ggccgccgcg
     6181 gccgccgcgt tcgccgtcct ctacctgccg tactcgctgg tcggcccgtt cgccggggtg
     6241 ttcatcgatc gctggtcccg gcggcagatc ctggtctggt cagccctgat ccgggcggcg
     6301 ttcgtggtg
//
LOCUS       DASWGG010000321         1952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3186362, whole genome shotgun sequence.
ACCESSION   DASWGG010000321 DASWGG010000000
VERSION     DASWGG010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3186362"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /gene="xseB"
                     /locus_tag="VF954_05765"
     CDS             <1..226
                     /gene="xseB"
                     /locus_tag="VF954_05765"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX9530624.1"
                     /translation="DTWNEDDGYDELSFEQLLSLLEELIERMASADVGIEEATTLYER
                     ADHIHALATGRLNQVRERVERLAPGGPTGG"
     gene            complement(168..1334)
                     /locus_tag="VF954_05770"
     CDS             complement(168..1334)
                     /locus_tag="VF954_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9530625.1"
                     /translation="MGGGSHGAHVFAVEDTSVQVVFAHLPAGDWQVEAGGQIARLHQA
                     RRGPAAATVHGLPAATRIDVTLSGPGVPRTPVARARTLEPPPGRLLSRLATMSDIHLG
                     SYAFGLRNTITEDPEPGWHPFPWRCAHAALTEAVAWGAEMLVVKGDLTATGRRSQLND
                     VGQLFSAAPVPVHLMLGNHEITRRGTDPWAVLAAWGLDTLRDVRVIDQPGIRVVLAHT
                     PAPISGRGHLTAPVKAKVLEAAAGAPGGVLLLLHHYLQAWPLPTFYPISIPSPVANPF
                     LAALGRSHPDTLISSGHSHRNRRYERRGLTLTEVASTIHYPGVWAGYAIYEGGIRQVV
                     RRIVEPSATEWVERTRRAVGGFWGRWAAGRLSDRCFSLLWAHPAPAAPPAPGPD"
     gene            complement(1346..>1952)
                     /locus_tag="VF954_05775"
     CDS             complement(1346..>1952)
                     /locus_tag="VF954_05775"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HEX9530626.1"
                     /translation="DPGTVARAGAIHRSLLEDRQAVEPNLAGAVVGVVAHSGGATEHR
                     QFVDRWHEAATPQEEIRYLYALADFPSAELMAETLRIAGAEARTQNAPFLIARALANP
                     RAGQVAWAHVRDHWDELLARLPDATVSRMLGGIVWLWDDAGLLAPEVETFLDTHPFPP
                     GDKTIAQLRDRLMVNATFARRRGDAVRAALGAASRPAAAAG"
BASE COUNT          266 a          687 c          759 g          240 t
ORIGIN      
        1 cgacacgtgg aacgaagacg acggttacga cgagctcagc ttcgagcagc ttctctccct
       61 gctcgaggag ctgatcgagc gcatggcgtc cgccgacgtg ggcatcgagg aggccacgac
      121 tctctacgag cgggccgacc acatccacgc cctggccacc ggccggctca atcaggtccg
      181 ggagcgggtg gagcggctgg cgccgggtgg gcccacagga ggctgaagca gcggtcgctc
      241 aggcggcccg ccgcccaccg cccccagaac ccccccacgg cgcgccgggt acgctccacc
      301 cactcggtgg cgctgggctc cacgatccgg cgcaccacct ggcggatgcc gccctcgtag
      361 atggcgtagc ccgcccacac gcccggatag tggatggtcg aggccacctc ggtcagcgtc
      421 aggccccggc gctcgtagcg acggttcctg tggctgtggc cgctcgagat cagcgtgtca
      481 ggatgggacc ggccgagggc ggccaggaac gggttggcca ccggcgacgg gatggagatc
      541 gggtagaagg tcggcagcgg ccaggcctgg aggtagtggt ggagcaagag caggacgccg
      601 cccggggcgc cggcggcggc ctcgaggacc ttggccttga ccggggccgt taggtgcccc
      661 cgcccggaga tcggggcggg cgtgtgggcc agcaccaccc ggattccggg ctggtcgatc
      721 acccgcacgt cgcgaagggt gtcgaggccc cacgccgcca gcaccgccca cggatcggtc
      781 ccgcgcctgg tgatctcgtg gttgccgagc atcaggtgca ccgggacggg cgcggccgag
      841 aacagctggc ctacgtcgtt gagctggctg cgccttcccg tggccgtcag gtcgcccttg
      901 acgacgagca tctcggcgcc ccacgccacc gcctcggtga gggccgcgtg ggcgcagcgc
      961 cacgggaacg ggtgccagcc cggctcgggg tcctccgtga tggtgttgcg gagcccgaag
     1021 gcgtacgagc cgagatggat gtcgctcatg gtggccagcc ggctcaacag ccggccggga
     1081 ggcggttcga gcgtgcgtgc ccgggcgacc ggcgtgcgcg gcacgcccgg gccggagagg
     1141 gtgacgtcga tgcgggtcgc ggccggcaac ccgtggaccg tcgctgccgc cgggccccgg
     1201 cgggcctggt ggagccgggc gatctgaccg cccgcctcga cctgccagtc gcccgccggg
     1261 aggtgggcga acacgacctg caccgaggtg tcctcgacgg cgaacacgtg cgccccgtga
     1321 gagccgccgc ccaccggagg caggctcagc ccgcggccgc ggcagggcgc gacgctgcgc
     1381 ccagggccgc acgcaccgcg tcgccgcggc gccgggcgaa ggtggcgttg accatgagcc
     1441 ggtcgcggag ctgggcgatg gtcttgtcgc ccggcgggaa cggatgggtg tcgaggaagg
     1501 tctccacctc gggggcgagc aggccggcgt cgtcccacag ccacacgatg ccgcccagca
     1561 tgcgcgagac cgtggcgtcg gggagacggg cgagcagctc gtcccaatgg tcccggacgt
     1621 gggcccacgc cacctgaccg gctcgggggt tggcgagggc gcgggcgatc aggaacggcg
     1681 cgttctgggt gcgcgcctcg gccccggcga tccgcagggt ctcggccatc agctcggccg
     1741 aggggaagtc cgccagggcg tagagatagc ggatctcctc ctgcggggtg gccgcctcgt
     1801 gccagcggtc gacgaactgg cggtgctccg tcgcgcctcc cgagtgggcc accacgccga
     1861 ccaccgcgcc ggccaggttg ggctcgaccg cctggcggtc ctcgagcagc gaccggtgga
     1921 tggcgcccgc ccgggcgacg gtcccggggt cg
//
LOCUS       DASWGG010000322         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2857746, whole genome shotgun sequence.
ACCESSION   DASWGG010000322 DASWGG010000000
VERSION     DASWGG010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2857746"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="VF954_05780"
     CDS             <1..1168
                     /locus_tag="VF954_05780"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9530627.1"
                     /translation="GGGGVGAGRRRGGGVTMRVGINAEPLFQRIPTGVGVYALALCEG
                     LVEIGHGDDLVLFHADHDEAPSEVGALSIDRVAFTLSRDRLYDAWLADRRPAPQSITG
                     DLDVVHSTGPAIPPSGGAVLVVTVHDLAPIRFADRYPRRARALHKRGAHIAAAEAARI
                     ICPSRSTALDVEEFYGVERDRIRVVPHGVDVAGLGVSDAGRRWERRGIAEPYVLWVGT
                     QEQRKNVVAVLDAFTHLAGRHPELSLVLHGPNGWLGDEVGEGLQHRGLHGRTIVSEGS
                     LPRNELAALYARASVFVYPSLYEGFGMPVLEAMACGTPVVTSNISALPETAGDAALLV
                     DPLDDVALAEAIARILEDPVLAEDLSRRGQKRANALTWGETARRTWAIYEEARG"
     gene            1161..>1627
                     /locus_tag="VF954_05785"
     CDS             1161..>1627
                     /locus_tag="VF954_05785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9530628.1"
                     /translation="MADGWTAVVVNYNAGAALAACVASILAEDPRPELVVVDNASADG
                     SVEGLLRAHPGVRVVRSGGNLGYARAANLGIAATGAPVVAVLNPDTVLTPGVGAALAA
                     RFAAEPDLGAVGPRLHNQDGTVYPSARRIPTMPDAVGHGLLFFVWRDNPFTRR"
BASE COUNT          194 a          649 c          560 g          224 t
ORIGIN      
        1 tggcggcggc ggcgtcggag ccggccgccg ccggggcggg ggcgtgacga tgagggtcgg
       61 gatcaacgcc gaaccgctct tccagcggat cccgaccggg gtcggcgtct acgccctggc
      121 cctgtgcgag ggcctggtgg agatcggcca cggcgacgac ctcgtccttt tccacgccga
      181 ccacgacgag gccccgtccg aggtcggggc cctctccatc gaccgggtcg ccttcacgct
      241 cagccgcgac cggctgtatg acgcatggct ggccgatcgg cgaccggcac cgcagtcgat
      301 caccggcgac ctcgacgtcg tccactccac cgggccggcc atcccgccgt ccggcggggc
      361 ggtgttagtg gtgaccgtcc acgacctggc cccgatccgc ttcgccgacc gctacccccg
      421 ccgggcccgg gccctccaca agcgcggcgc ccacatcgcc gccgccgagg cggcccgcat
      481 catctgcccg tcccgctcca ccgccctcga cgtcgaggag ttctacggcg tcgagcggga
      541 tcggatccgg gtcgtaccgc acggcgtcga cgtggccggg ctcggtgtct ctgacgccgg
      601 gcggcgctgg gagcgccggg ggatcgccga gccctacgtc ctgtgggtcg gcacccagga
      661 gcagcgcaag aacgtcgtcg ccgtgctcga cgccttcacc cacctggccg gccgccaccc
      721 cgagctgtcg ctcgtcctcc acggccccaa cggctggctg ggcgacgagg tgggggaggg
      781 gctgcagcac cggggcctcc acggccgcac gatcgtcagc gagggctcgc tgccccgcaa
      841 cgagctcgcc gccctctacg cccgggcgtc ggtgttcgtc tacccctcgc tctacgaggg
      901 gttcgggatg ccggtgctgg aggccatggc ctgcggcacc ccggtcgtga cctccaacat
      961 ctcggcgctg cccgagacgg ccggcgacgc cgccctgttg gtcgaccccc tcgacgacgt
     1021 cgccctggcc gaggcgatcg cccgcatcct cgaggatccg gtcctggccg aggacctgtc
     1081 gcgccggggg cagaagcggg ccaacgccct cacctggggc gagaccgccc gccgcacttg
     1141 ggccatctat gaggaggctc gtggctgacg gctggaccgc cgtcgtcgtc aactacaacg
     1201 ccggggccgc cctggcggcc tgcgtcgcct cgatcctggc cgaggacccc cgcccggaac
     1261 tggtcgtggt cgacaacgcc tcggccgacg gttccgtcga agggctcctc cgggcccatc
     1321 ccggcgtgcg ggtcgtgcgc tcgggcggca acctcggcta cgcccgggcc gccaacctcg
     1381 gcatcgccgc caccggcgct ccggtggtgg cggtcctcaa ccccgacacg gtgctgaccc
     1441 ccggggtggg cgccgccctg gccgcccgct tcgccgccga gcccgacctc ggcgccgtcg
     1501 ggccacgatt gcacaaccag gatggcaccg tgtacccgtc ggcccggcgg atcccgacca
     1561 tgcccgacgc cgtcggccac ggcctcctct tcttcgtctg gcgggacaac ccgttcaccc
     1621 gccgcta
//
LOCUS       DASWGG010000323         2571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_931148, whole genome shotgun sequence.
ACCESSION   DASWGG010000323 DASWGG010000000
VERSION     DASWGG010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931148"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VF954_05790"
     CDS             <1..879
                     /locus_tag="VF954_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530629.1"
                     /translation="NGGCISATDPRQDLDQIGTGQEMFRAYFFQSWATTPTGQRDWTA
                     MDRTVAAAAAHGERVVAVLANEWAYCDGPQKFLGWWQSGYRTTVSPGEATTYRQWVSD
                     VVSRYADNPAIGIWDLVNEGEGRAFDGICAEAAATAALSAFSNDVGSLVHSIDRQHVV
                     TLGTVSGQCGSNEGDYATIYSSAGSDLCDYHNYGAPTVPMGNSDPYNGLQVSIDRCHA
                     DGKPLAVLETGIHYTQLSPPTTANRADLFAQKFAAQFSAGVVGELLWDYSPNPQVRWD
                     YEIGPNDPALGVLSRF"
     gene            940..2544
                     /locus_tag="VF954_05795"
     CDS             940..2544
                     /locus_tag="VF954_05795"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX9530630.1"
                     /translation="MSPGHYVEIFLRRWPVLAVCIVASVIGAVAVTAVSAKQYEASSR
                     VFIDVPVPQDVGQALQGFELSSQLLSSYTKIATSSTALGRISARVGGRLSPDQVRSRL
                     TVSSVTGTLLIDVKARDSHPQVARDLADAGAATLQQMVEQLDPTRQNPVDAKIIDPAS
                     LPTTPVRPRPRVNVLLGGLVGLFAGAGLVVALESLDRAVRTPAQAAVAFGAPVLATIP
                     RDRRLQGAPVIPAERSTTPIGEAFRSLRTAIRHLDDDGLRTLLVSSPVAGAGTTTVAA
                     NLATAIAAAGERAILIDADLRQPRLSTWFGLADRAGLTSVLLGEATLSDALHWVGPRL
                     SVLPSGPVPQNPAELVRSQRMVEVLVAASELADVVVVDAPPVLTAADAAELGALADGV
                     LLVVRSGATDAAAGPATVSVLAPVGGRIVGLALNDTRGRRPGPYAPRPSAPPEHTPAE
                     LAGLVRDAVASALVANPAPPGEARSSRSQPPGAQPAPATRGVTRRGAPSRSAKPVAKP
                     VAKPTATRARRRSAPPPDDPPGRDQG"
BASE COUNT          344 a          987 c          868 g          372 t
ORIGIN      
        1 aacggcggct gcatctcggc caccgacccc cgacaggacc tcgaccagat cggcacggga
       61 caggagatgt tccgtgccta cttcttccag agctgggcca ccaccccgac cggtcagcgg
      121 gattggacag cgatggaccg gaccgttgcc gcggcggcgg cccacgggga acgggtggta
      181 gccgtgctgg cgaacgagtg ggcctactgt gacgggcccc agaagttcct cggttggtgg
      241 cagtccggct accgcaccac cgtcagcccg ggcgaggcga ccacctaccg gcagtgggtc
      301 agcgacgtgg tgtcgcggta tgccgacaac ccggccatcg gcatctggga cctggtcaac
      361 gagggcgagg ggcgcgcctt cgacggcatc tgcgccgagg cggcagccac tgcggccctg
      421 tccgcgttca gcaacgacgt cggctcgctc gtgcactcca tcgaccgcca gcacgtggtc
      481 accctgggca ccgtctccgg ccagtgcggc tcgaacgagg gcgactacgc cacgatctac
      541 tcctcggccg gctccgacct gtgcgattac cacaactacg gggcacccac cgtgcccatg
      601 ggcaacagcg acccttacaa cgggttgcag gtgagcatcg atcgctgcca tgccgacggc
      661 aaacccttgg cagtgctgga gaccggcatc cactacaccc agctgtcgcc tccgaccacc
      721 gccaaccggg cagatctgtt tgcccagaag ttcgccgcgc agttctcggc gggcgtcgtc
      781 ggcgagctct tgtgggacta ctcacccaat cctcaggtcc ggtgggacta cgagatcggg
      841 cccaacgacc cggcgctggg tgtgctcagc cgtttctgac cagctcggcc atgatggcga
      901 tccgtccccg cgaccgccga ccctctaccg tgtcccaccg tgagccccgg gcactacgtc
      961 gagatcttcc tccgccgatg gccggtcctt gccgtctgca tcgtcgcctc ggtcatcgga
     1021 gccgtcgccg tcacggccgt gtcggccaag cagtacgagg cctccagccg ggtgttcatc
     1081 gacgtccccg tcccccagga cgtcgggcag gcgctgcaag gattcgagct gtcctcacag
     1141 ctgctgtcca gctacaccaa gatcgcgaca tcgagcaccg cgctcggtcg gatctccgcc
     1201 cgggtgggcg gacgtctctc gcccgaccag gtgcgtagcc gcctcaccgt ctcctcggtg
     1261 accgggacgc tgttgatcga cgtcaaggcc cgggactcgc atccccaggt ggcgcgcgac
     1321 ctggccgacg cgggcgcggc caccctccaa cagatggtgg agcagctcga cccgacgcgg
     1381 cagaacccgg tcgacgccaa gatcatcgac cccgcctcgc tgcccaccac acccgtacgg
     1441 ccccgccctc gggtcaacgt cctcctcggg ggcctcgtgg gactcttcgc cggggccggt
     1501 ctggtcgtcg ccctcgagtc tctcgatcga gccgtccgga cacctgccca ggcggccgtg
     1561 gccttcggcg cccccgtgct ggccacgatc cctcgcgacc ggaggcttca gggtgccccg
     1621 gtgatcccag ccgagcggtc gaccaccccg atcggcgagg cattccggtc gctccgcacg
     1681 gccatccgcc acctggacga tgacggcctc cggacgctgc tggtctcgtc gccggtggcc
     1741 ggggccggca ccaccacggt ggccgccaac ctggcgaccg ccattgccgc ggcgggagag
     1801 cgggccatcc tgatcgacgc cgacctgcgc cagccgcgcc tatcgacctg gttcggcctc
     1861 gccgatcgtg ccggcctgac gtcggtgttg ctgggcgagg cgacgctgtc cgacgcgctg
     1921 cattgggtcg ggccgcggtt gagcgtgctg ccgagcgggc ctgtgcccca gaaccccgcc
     1981 gagctggtcc ggagccagcg catggtcgag gtcctggtgg cggcctcgga gctggccgac
     2041 gtggtggtgg tggacgcgcc tccggtgctc acggcggcgg acgctgccga gctgggcgcc
     2101 cttgcggacg gcgtgctgct ggtggtgcgg tcgggcgcga ccgacgccgc ggcggggccg
     2161 gcgaccgtgt cggtgctcgc gcccgtcggg gggcggatcg tcgggctcgc cctcaatgac
     2221 acccggggcc ggcggccggg cccgtacgcg ccccgcccct ccgctccgcc tgagcacacc
     2281 cctgccgagc tggccggcct ggtccgcgac gcggtggcgt cggccctggt ggccaacccc
     2341 gcgccgcccg gtgaggcccg gtcctccagg tcacagcccc ctggggcgca gcccgcccca
     2401 gccacgaggg gcgtcacccg ccgcggcgcg ccctcgcgct ccgcgaagcc tgtcgccaag
     2461 cctgtcgcca agcccacggc tacgcgggcc aggcgcaggt ccgccccgcc gccggacgac
     2521 ccgcccggcc gggaccaggg ctgacccagc ccgctcacgc gttctcgcag c
//
LOCUS       DASWGG010000324         3345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1903796, whole genome shotgun sequence.
ACCESSION   DASWGG010000324 DASWGG010000000
VERSION     DASWGG010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903796"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VF954_05800"
     CDS             complement(<1..677)
                     /locus_tag="VF954_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530631.1"
                     /translation="MPDTPAFDTPTSDPPPVGVSTAAPAKTGLTRRRFLAGGLAAGAV
                     GAAGAVVARHLAAPAPAHHRGLSGAGTLVLVTLYGGNDGLNTVIPYEDGSYLGGRAQL
                     GYQPNEVLPLADGLGLHPNLKGLKGLWEARQLAIVRGVGYPNPNRSHFRSMDIWQSAA
                     PDRAVGSGWLGRWLDTTSRDPLLGLAVGPTLPLALTGDKVLGGAVPVGNLRLPGTTAL
                     QQGYASL"
     gene            complement(696..1997)
                     /locus_tag="VF954_05805"
     CDS             complement(696..1997)
                     /locus_tag="VF954_05805"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX9530632.1"
                     /translation="MAEDDIRAQVAHLYRRAGFGATAAELDQAASQGVEATVAKLMAG
                     LGEPDHGADAVADPLLGAAGAGAVGGAGQDRAARAQLAADRKALALWWLDRMAASTGP
                     LREKLALLWHGHFATGIQKVRLAAYMFRQNQIFRALGAGSFETLTQAVAKDPAMLVWL
                     DSNTNVRSHPNENFARELMELFTLGIGNYTEDDVKEAARCFTGWAVNRSADSFVFRAN
                     QHDGGVKTVLGRSGNFGGEDVVTVLTRSPAAARLVPARLWSHLAYPVSATDPVVSELA
                     TGYARDLSVDSLVRAILSHRKFMAPQARTGLVKQPVEYVVGAFRALGLKPSEHPEVLA
                     ALAGLGQVPFEPPSVGGWLQNGYWLSTAAALTRLRFAALVAGRADLSAIAGAAPAARP
                     AALERLLSVNWTPSTANTLANATDPRLVVALGLVAPEYVLN"
     gene            2117..2557
                     /locus_tag="VF954_05810"
     CDS             2117..2557
                     /locus_tag="VF954_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530633.1"
                     /translation="MDELDPFKVLGLPDGATPADASDAYRRRVALAEEARRLAPSAED
                     RARIERQQLLLARAYEMIDAELEDGPSRMLIGPADRPPALLASIALGAVGLAILVVAL
                     AAGSSAVYVLAIFCGAASLASALIWRHQLVESWAARGLPRSRTS"
     gene            complement(2474..3070)
                     /locus_tag="VF954_05815"
     CDS             complement(2474..3070)
                     /locus_tag="VF954_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX9530634.1"
                     /translation="MNREEALAVLGVNGQPDGAALRRAYRRLLRRHHPDVAGADTQAA
                     TVRIIQAYRVLRGQADAEPAPAPPPSPPPPGGRAEGDAVCLALPPHEAFLLLLDVAQR
                     YGEVTYLDRENGLFETVIEFEGYGPCSVMATMQGRATGVTEAFLCCEPLIPSRPSPAI
                     PEVAAWLSAACSGGAAPGEAPGRPGLDQLVPPDQGGCQ"
     gene            complement(3128..>3345)
                     /locus_tag="VF954_05820"
     CDS             complement(3128..>3345)
                     /locus_tag="VF954_05820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9530635.1"
                     /translation="RFGHELAVVTEDGHREGGLARRLVAQAARRVVAEGAVPTYLHSD
                     SNVASAHVAEAAGFADQGWRVVSLGRG"
BASE COUNT          442 a         1209 c         1242 g          452 t
ORIGIN      
        1 tcgagggagg cgtagccctg ctggagggcg gtggtgccgg gcagcctgag gttcccgacc
       61 ggaacggcgc ccccgagaac cttgtcaccc gtcaaggcca ggggcagcgt cggaccgacg
      121 gccaggccca gcagcgggtc cctggacgtt gtgtctagcc accgaccgag ccagccagat
      181 cccacggccc ggtcgggcgc cgcgctctgc cagatgtcca tcgaccggaa gtggctccgg
      241 ttcgggttcg ggtagcccac gccgcgcacg atggcgagct ggcgcgcctc ccacaggccc
      301 ttgaggccct tgaggttggg gtgcaggccc agcccgtcgg cgagggggag cacctcgttc
      361 ggttggtagc cgagctgggc ccggccgccc aggtagctgc cgtcctcgta cgggatgacg
      421 gtgttgagcc cgtcattgcc accgtagagg gtgaccagga ccagcgttcc cgcacccgaa
      481 aggccccggt gatgcgccgg cgccggtgcg gccaggtgcc gggccacgac ggcgccggcc
      541 gctccgaccg cgccggccgc caggcccccg gcgaggaacc ggcggcgcgt gagcccggtc
      601 ttcgccgggg cggccgtgga gacacccacg ggcggggggt cggaggtcgg ggtgtcgaat
      661 gccggggtgt cgggcatggg cgctcgtggg tctcttcagt tgaggacgta ctcgggggcg
      721 accaggccca gggcgacgac gagacgggga tcggtggcgt tggcgagcgt gttggccgtc
      781 gagggcgtcc agttgaccga caggaggcgc tccagcgcgg ccgggcgggc ggccggggcc
      841 gcgccggcga tggccgacag gtcggcgcgc cccgccacca gggcggcaaa gcgcagccgg
      901 gtcagcgcgg ccgcggtgga cagccaatag ccgttctgga gccacccgcc gacgctcggc
      961 ggctcgaacg ggacctggcc caacccggcc agagccgcca ggacctcagg gtgctccgac
     1021 ggcttgagcc caagggcgcg gaaggctccg acgacgtact cgacgggttg cttcaccaac
     1081 cccgtccgag cctgcggggc catgaacttc cggtgcgaca ggatggcccg cacgagcgag
     1141 tccacgctca ggtcgcgggc atagcccgtg gccagctcgc tcaccaccgg gtcggtggca
     1201 cttaccgggt acgccaggtg gctccacagc ctggccggga cgaggcgggc cgcagcgggg
     1261 ctgcgggtca gcacggtgac gacgtcctcg cccccgaagt tgccgctacg gcccagcacg
     1321 gtcttgacgc ccccgtcgtg ctggttggcc cggaagacga agctgtcggc ggagcggttc
     1381 acggcccatc cggtgaagca gcgggcggcc tccttgacgt cgtcctccgt gtagttgccg
     1441 atgccgaggg tgaacagctc catcagctcg cgggcgaagt tctcgttcgg atgggacctg
     1501 acgttcgtgt tggagtcgag ccagacgagc atggccgggt ccttggcgac ggcctgggtg
     1561 agggtctcga agctgcccgc gcccagcgcc cggaagatct ggttctgccg gaacatgtac
     1621 gcggccagcc ggaccttctg gatccccgtg gcgaaatggc cgtgccagag gagggccagc
     1681 ttctcccgca gcggcccggt ggacgcggcc atgcggtcga gccaccacag cgccagcgcc
     1741 ttgcggtccg cggcaagctg cgctcgcgcc gccctgtcct ggccggcgcc cccgaccgcg
     1801 ccggcgccgg ccgcgccgag caagggatcg gcgacggcgt cggccccgtg atccggttcc
     1861 ccgaggcccg ccatcagctt ggccacagtg gcttccacac cctggctcgc ggcctggtcg
     1921 agctccgccg cggtggcgcc gaagccggcc cggcggtaga ggtgtgcgac ctgtgctcgg
     1981 atgtcgtcct cggccattgc cctcttcctc cgggaccagg ctgcacgaag ggtatgaggg
     2041 aagtgtgacc gggtggtggc gcgccggtca gggccggcta ccgctctcga ccctagcgcc
     2101 ggtagcctcc tgggcgatgg atgagctcga tccgttcaag gttctgggct tgccggacgg
     2161 agcgactccg gccgacgcct ccgacgccta caggcggcgg gtcgccctcg ccgaggaggc
     2221 ccgtcgcctg gcaccatccg ccgaggaccg ggcgcggatc gagcgccagc agctgctgct
     2281 ggcccgggcc tacgagatga tcgatgccga gctggaggac gggccgtccc ggatgctgat
     2341 cggaccggcc gaccgcccgc cggccctgct cgcctccatc gccctcggag cggtcgggct
     2401 ggcgatcctg gtcgtggccc tcgccgccgg atcctcggcg gtgtacgtcc tggccatctt
     2461 ctgcggagcg gcctcactgg catccgccct gatctggcgg caccagctgg tcgagtcctg
     2521 ggcggcccgg ggcctccccc ggagccgcac ctcctgagca ggctgcgctc agccacgcgg
     2581 ccacctccgg gatcgccggg gatggccggc tggggatcag cggctcgcag cagaggaacg
     2641 cctcggtcac gcccgtggct cgcccctgca tcgtcgccat gaccgagcat gggccgtagc
     2701 cctcgaactc gatcaccgtc tcgaacagcc cgttctcccg gtccaggtac gtcacctccc
     2761 cgtagcgctg ggccacgtcg agaaggagca ggaaggcctc gtgcggcggg agagcgagac
     2821 agacggcgtc cccttcagcg cgcccgcccg gcggcggcgg ggacggcggc ggcgcgggtg
     2881 cgggctcggc gtcagcctgg ccgcgcagca cccggtacgc ctggatgatc cggaccgtcg
     2941 ccgcctgggt atcggccccc gccacgtcgg gatggtggcg ccggagcagg cggcgatacg
     3001 cccggcggag agccgcgccg tcgggctggc cgttcacacc cagcacggcg agggcctctt
     3061 cccggttcat ggctcactcc cgcaggccgt cctgcaagct gcgtcgccgg gcgcgggcgc
     3121 gccgcgatca gccacgaccc aggctgacca cccgccagcc ctggtcggca aagcccgcgg
     3181 cctcggcgac atgggccgag gccacgttgc tgtccgagtg caggtaggtc gggacggcgc
     3241 cttcggcgac gacccggcgg gccgcctgtg cgaccaaccg ccgcgccagg ccgccctccc
     3301 ggtgcccatc ctcggtcacc accgccagct catggccgaa ccggt
//
LOCUS       DASWGG010000325         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1262998, whole genome shotgun sequence.
ACCESSION   DASWGG010000325 DASWGG010000000
VERSION     DASWGG010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262998"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1402)
                     /gene="murD"
                     /locus_tag="VF954_05825"
     CDS             complement(5..1402)
                     /gene="murD"
                     /locus_tag="VF954_05825"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX9530636.1"
                     /translation="MAPVLVIGLGVTGEAVARRMVADGDHVVVVDDRASPERRALADA
                     VGVELVEAPGPAALAALVGAADVVVVSPGVPVSHPVFAVPGADLVSEVELAARRCRAP
                     IVGVTGTNGKTTVTTLITDMLLHAGRRAVAAGNIGTALIEVVGGGPPGPGGAPLSGAA
                     APEVVVAPEVVVAELSSFQLFLTSSFRPAVSVWLNFTEDHLDWHPSLEHYRAAKARIW
                     ANTHDDDVVIGNADDPVVSAELARAGGRPVTFGLAAQGLVGVPHYGLADGWLVTPEGE
                     RVAAADELPRALPHELANGLAATAAAVAAGATPADCRAVLLRFAGLPHRIELVGESGG
                     VRWYDDSKATTPASVVAALRGFPSAVLIAGGRNKGLSFARLRAEAPRLRAAVAIGEAA
                     GEIDLALHGVCPVVRAPSMTEAVAAAARLAHPGDAVLLSPGAASFDWYDSYAERGDDF
                     ARAVREHHQTEGASR"
     gene            complement(1402..>1670)
                     /locus_tag="VF954_05830"
     CDS             complement(1402..>1670)
                     /locus_tag="VF954_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX9530637.1"
                     /translation="ALKLNLDLLLPLVCALFVLETVSVILQVFAFRVFHRRVFLMAPI
                     HHHFELRGWPETTVTIRFWIMAALCTALALGIFYADFLTTGRID"
BASE COUNT          252 a          610 c          621 g          187 t
ORIGIN      
        1 ccgatcatcg agatgccccc tcggtctggt gatgctcgcg gaccgcccgg gcgaagtcgt
       61 cgccgcgctc ggcgtaggag tcgtaccaat cgaagctcgc cgcgccgggc gagagcagca
      121 ccgcgtcgcc ggggtgggcc aggcgggcgg ccgcggcgac ggcttcggtc atcgacggcg
      181 cccgcaccac ggggcacacg ccgtggaggg ccaggtctat ctcgccagcg gcctcgccga
      241 tggccaccgc ggcccgcagc cgcggcgcct cggcgcggag ccgagcgaag ctcaggccct
      301 tgttgcggcc gcccgcgatg agcactgcgg aagggaaccc cctcagggcg gcaacgaccg
      361 atgccggggt cgtcgccttg gaatcgtcgt accaccgcac cccaccagat tcgcctacca
      421 gctcgatccg gtgggggagc cccgcgaacc gcagcagcac ggcccggcag tcggccgggg
      481 tggcgccggc cgcgaccgcc gccgcggtgg ccgccagccc gttggccagc tcgtggggca
      541 gggcgcgcgg cagctcgtcc gcggcggcca cgcgctcgcc ctcgggcgtc accaaccatc
      601 cgtctgccag cccgtagtgc gggaccccga ccaggccctg ggcggccagg ccgaacgtga
      661 cgggccgccc accggcgcgg gccagctcgg ccgaaacgac cgggtcgtcg gcgttgccga
      721 tcaccacgtc gtcatcgtgg gtgttggccc agatcctggc cttggccgcc cggtagtgct
      781 cgagcgacgg atgccagtcc aggtggtcct cggtgaagtt cagccagacc gagacggcgg
      841 ggcggaacga cgaggtcagg aacagctgga agctggacag ctcggcgacc acgacctccg
      901 gggcgaccac gacctccggg gcggccgccc ccgacagcgg cgccccgccg ggcccgggcg
      961 gcccaccgcc gaccacctcg atcagcgccg tgccgatgtt gccggcggcg acggcgcgcc
     1021 ggccggcgtg gagcagcatg tcggtgatga gggtggtcac ggtggtcttg ccgttggtgc
     1081 ccgtgactcc gacgatcggg gcccggcagc ggcgggcggc cagctccacc tcgctcacca
     1141 ggtcggcgcc cgggacggcg aacacgggat gggacaccgg aacgcccggg ctgaccacga
     1201 ccacgtcggc ggccccgacg agtgcggcca gggcggccgg gcccggtgct tcgaccagct
     1261 ccacgccgac cgcatcggcg agcgccctgc gctccgggct ggcccggtcg tcgacgacca
     1321 cgacgtggtc cccgtcggcg accatccgcc gggcgacggc ctcaccggtc acgccgagcc
     1381 cgatgacgag gaccggcgcc atcagtcgat ccggccggtg gtgaggaagt cggcgtagaa
     1441 gatgcccagg gcgagggcgg tgcagagagc ggccatgatc cagaagcgga tggtgaccgt
     1501 cgtctccggc caccccctga gctcgaagtg gtggtggatg ggcgccataa ggaacacccg
     1561 ccggtggaac acccggaagg cgaacacctg gaggatcacc gacacggtct ccagcacgaa
     1621 cagggcgcac accagcggca gcaacaggtc gaggttgagc ttcagggcga
//
LOCUS       DASWGG010000326         4225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_933861, whole genome shotgun sequence.
ACCESSION   DASWGG010000326 DASWGG010000000
VERSION     DASWGG010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933861"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..2189)
                     /locus_tag="VF954_05835"
     CDS             complement(66..2189)
                     /locus_tag="VF954_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530638.1"
                     /translation="MIRERGGPAPAGLPGPEALERVGSDWLRVGGALCVTLKVASWPR
                     EVSPGWLTPLLKLGVAVDLALHVEPEAPEAAVAHLRRQFTRMRTTQISAEDGGDLPDP
                     RVEASADDAQRMHAAIGRDVTRSAQVGLYLTVWADTPEALQTAAARVRARASSMLLEL
                     RPTSFRPKSGWLSTQPLCLDLVGGRRRFDTAPLAATLPLSTGEVRSDPGGSFAGLNLS
                     TGTPVFVDRFALDAHRPNFNKLSVAGSGRGKSFESKHEAISLLLEGVDVLIVDPENEY
                     LRLADALGGTVLRLGVDGARVNPLELAEAGQSSAVTEQALFVGSLVATLTGPLSATGR
                     AQLDTAILATYRAAGISADPATHDRTPPVLADLLGCLESAGAGDLATRLGPWVSGAYR
                     GLFSGQTTVAAEGRLVVYALGDLPEEEEALMAAGILLAAQGIWRAVARGPRRRRVVIL
                     DEAWKITGASQAAGVVLQRLVRRLAKGARKYLAGINAVTQDLEEFLETPLGRTVLNNS
                     AIRWLPGQEAQALPLVARTFGLTEADARFLRTAARGQGLLVFGRQRVRLGVVATPGEH
                     RLCTSDPEELAVLERQDLGEEVDAALEAFFRVLSAASRHLDADILPEVATGPALILLV
                     AEIEGLVARRSPIGLGISYRVVELTGDRLAGRAAASLELRRRRQVLDPAGAGPARGHT
                     AESWAETATYNLRRLGGTWKVTGIA"
     gene            complement(2186..3109)
                     /locus_tag="VF954_05840"
     CDS             complement(2186..3109)
                     /locus_tag="VF954_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEX9530639.1"
                     /translation="MRELDAMRAEVPADTNLPDQLVFGLSAHQLLVVVPAVVSGVGLF
                     FLGRLLPTWALVGAVAAWVILAMASLAFAVADHDGEALDSYLAQVLRERRRPRAEVQA
                     PGGLPALPAWAGGARPDAPGPMTLPWEGVDPAGHIALGRDERGRRLGVACVLAASPLD
                     YEAMAPADLEAFCQSAEEVLKSLEAPLQLLVRAEAQDLAGPVGALRARAGELGAGALG
                     RIAAERAGMLEAVAGEAHMALSSYLCVRAEDTAALDDRVAHLSESLGRLDVALRRLGC
                     GELEALLRRGALGPGPSLAGQAGAGRERRSA"
     gene            complement(3106..>4225)
                     /locus_tag="VF954_05845"
     CDS             complement(3106..>4225)
                     /locus_tag="VF954_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530640.1"
                     /translation="PGPATSPGAAASPGPDVPSATPQPAATQPGAPTATTTPGTTSAG
                     SGGSHGGILGSIIGAVTDPGGAIVRSVGDYLSGLATSVIQPVFDYFARSLFHTPDLGA
                     NPSLRPLWFVSLAVVDAGVGLVIVIGALKLTWGGLRESLEAKEIFERLAVGAIGAHMS
                     LLVFGPLSSFSNALARAFIGVHPVDAKPQLLGMLATTTSGAGGSLSVLLVLAVAVVGI
                     LVIFWDVVRIITLALLVVAGPFGNFSYSLSETEGFATGWWRAVIYLSLVPVAQALLYS
                     VGLWLFLFSPTPVLSFGGSGLVEAMVLFVVLYLIAKIPVLALRAAAAPVHQAYSNSKR
                     AVRTVVLVAAFGRLPGFAGALGRIAGGRRAAGGAKAAP"
BASE COUNT          603 a         1533 c         1623 g          466 t
ORIGIN      
        1 gttccgccat ccccgggttt ggggtcgtgg aggtttcagc agtgctgagc tcacggcttc
       61 ccccctcatg cgatgccggt cactttccag gtaccaccca ggcgccgcag gttgtaggtg
      121 gcggtctccg cccaggactc ggcggtgtgg ccccgggcag gcccggcacc ggcgggatcg
      181 aggacctggc ggcgccgtcg cagctcgagc gaggccgccg ccctgcccgc gaggcgatcg
      241 ccggtgagct cgaccacccg gtaggagatg ccgagaccga tcggcgaacg gcgagcaacc
      301 agtccctcga tctccgccac gagcaagatc agcgccgggc cggtcgcgac ctcggggagg
      361 atgtcggcgt cgaggtgccg gctcgcggcg gagagcaccc ggaagaaggc ctccagggcg
      421 gcgtcgacct cctcgcccag gtcctggcgc tccagcaccg ccagctcctc ggggtcggag
      481 gtgcagaggc ggtgctcgcc gggcgtggcc acgacgccga ggcgcacgcg ctggcggccg
      541 aagacgagca ggccctggcc ccgggccgcg gtgcgcagga acctcgcatc cgcctcggtg
      601 aggccgaagg tcctggcaac gaggggcagc gcctgggcct cctggccggg cagccaccgg
      661 atggccgagt tgttgagcac cgtccgcccc aggggggtct ccaggaactc ctcgaggtcc
      721 tgggtgacgg cgttgatccc ggccaggtac ttgcgggctc ccttggcgag gcggcgcacc
      781 aggcgctgca gcaccacgcc cgcggcctgc gaggcgccgg tgatcttcca ggcctcgtcc
      841 aggatcacga cccgacgccg gcgcggcccg cgggcgacgg cccgccagat gccctgggcc
      901 gccagcagga tgccagcggc catgagggcc tcctcctcct cggggaggtc gcccagggcg
      961 tagacgacga ggcggccctc ggccgcgacg gtggtctggc cggagaacag cccgcggtag
     1021 gccccgctca cccagggacc gagacgcgtc gccaggtcgc cggcgccggc tgactcgagg
     1081 catcccagca ggtcggccag caccgggggg gtgcggtcat gcgtcgctgg gtccgcgctg
     1141 atccccgccg cccggtaggt cgccaggatg gccgtgtcca gctgggcacg cccggtcgcg
     1201 ctgaggggcc ccgtgagggt ggccacgagc gagccgacga acagtgcctg ctcggtgacc
     1261 gccgaggact gcccggcctc cgccagctcg agggggttca cccgggcccc gtccaccccc
     1321 aggcgcagca ccgtcccgcc gagggcgtcg gcgaggcgga ggtactcgtt ctcgggatcg
     1381 acgatgagca cgtcgacccc ctcgagcagc agggagatcg cctcgtgctt gctctcgaag
     1441 ctcttgcccc ggcccgaccc cgctaccgag agcttgttga agttgggccg gtgggcgtcg
     1501 agggcgaagc ggtccacgaa caccggcgtg cccgtcgaca ggttgagccc ggcgaaggag
     1561 ccacccgggt cggagcggac ctcgccggtg gacagcggca gggtggccgc cagcggggcg
     1621 gtgtcgaatc gccggcgccc gccgaccagg tccaggcaca gcggctgggt cgacagccac
     1681 cccgacttgg ggcggaacga ggtgggccgg agctcgagca gcatcgagga ggcccgggcg
     1741 cgcaccctgg cggcagcggt ctgaagcgcc tcgggcgtgt ccgcccagac ggtcaggtac
     1801 aggcccacct gggcgctgcg ggtgacgtcg cgcccgatgg cggcgtgcat gcgctgggcg
     1861 tcgtcggcgg aggcctcgac ccgggggtct gggagatcgc ccccgtcctc ggcggagatc
     1921 tgggtcgtgc gcatcctggt gaactggcgg cggagatgcg ccacggcggc ctcgggggcc
     1981 tcgggctcga cgtgcagggc caggtcgacc gccacgccga gcttgagcag cggggtgagc
     2041 caacccgggg agacctcccg gggccacgag gccaccttga gggtgacgca cagcgccccg
     2101 ccgacccgca gccagtcgct gcccacgcgc tcgagtgcct cggggccggg caggccggcg
     2161 ggggcgggcc ccccgcgctc gcggatcacg cggaccgcct ctcacgcccc gcaccggcct
     2221 gcccggccaa gctcggcccc gggcccagcg ccccccggcg cagaagggcc tccagctcgc
     2281 cgcagccgag gcgccggagg gccacgtcca ggcggccgag ggactcgctc aggtgtgcca
     2341 cccggtcgtc gagggcggcg gtgtcctccg cgcgcacgca gaggtagctc gacagggcca
     2401 tgtgggcctc gccggcgacg gcctccagca tcccggcgcg ctccgccgcg atgcgcccca
     2461 gcgcgcctgc accgagctcg ccggcccggg cccgcagtgc cccgacgggc ccggccaggt
     2521 cctgcgcctc ggcgcgcacc aggagctgca gcggtgcctc cagggacttg agcacctcct
     2581 cggcggactg gcagaaggcc tcgagatcgg ccggcgccat cgcctcgtag tccagggggg
     2641 aggccgccag cacgcaggcc acccccaggc ggcgtccccg ctcgtcgcgg ccgagcgcga
     2701 tgtggccggc ggggtcgacc ccctcccacg gcagggtcat cggcccgggc gcgtccgggc
     2761 gggcgccgcc ggcccatgcg ggcagggccg gcaggccgcc gggggcctgc acctcggccc
     2821 gtgggcgccg gcgctcgcgc agcacctgcg cgaggtagga gtcgagggcc tcgccgtcgt
     2881 ggtcggcgac ggcgaaggcg aggctggcca tggcgaggat cacccacgcc gcgacggccc
     2941 ccacgagtgc ccatgtgggg aggaggcggc cgaggaagaa gaggccgacg cccgacacca
     3001 ccgccggcac caccaccagc agctggtggg cggagagccc gaacaccagc tggtcgggga
     3061 ggttcgtgtc cgccgggacc tcggccctca tggcatcgag ctccctcatg gcgccgcctt
     3121 ggcgccgcct gcggccctgc gccccccggc gatgcgcccg agcgctccgg cgaagcccgg
     3181 aagccggccg aaggcggcga cgagcaccac ggtgcggacg gcccgcttgg agttggagta
     3241 ggcctggtgg acgggggcgg cggcggcccg gagggcgagc acggggatct tggcgatgag
     3301 gtacaggacg acgaacagca ccatggcctc caccaggccc gagccgccga aggagaggac
     3361 cggggtgggg gagaacagga acagccacag ccccaccgag tacagcagcg cctgggcgac
     3421 gggcacgagc gagaggtaga tcaccgcccg ccaccagccc gtggcgaagc cctccgtctc
     3481 cgacagggag tagctgaagt tgccgaaggg gcccgccacc acgagcaggg ccagggtgat
     3541 gatgcgcacg acgtcccaga agatgacgag gatcccaacg accgccacgg cgagcaccaa
     3601 cagcacgctg agggagccgc ccgcccccga cgtcgtggtg gcgagcatgc cgaggagctg
     3661 gggcttggcg tcgacggggt gcaccccgat gaatgcccgg gcgagggcat tggagaacga
     3721 gctcagcggc ccgaagacga gcaggctcat gtgcgccccg atggcgccca ccgcaaggcg
     3781 ctcgaagatc tccttggcct cgagggactc tctgaggccc ccccaggtga gcttgagcgc
     3841 cccgatgacg atcaccaggc ccacaccggc gtcgaccacc gccagggaga caaaccacag
     3901 cgggcgcagg ctcgggttgg caccgaggtc gggggtgtgg aacagggacc tggcgaagta
     3961 gtcgaagacc ggctggatga ccgaggtggc cagccccgac aggtagtcgc cgacgctgcg
     4021 cacgatggcc ccgccggggt cggtcaccgc cccgatgatc gagcccagga tgccgccgtg
     4081 cgagccccct gacccggcgc tggtcgtccc cggcgtggtg gtcgcggtcg gcgccccggg
     4141 ctgggtcgcg gcgggctggg gggtcgccga cggcacatcc gggccgggcg aggccgccgc
     4201 gccgggcgag gttgctggtc cgggc
//
LOCUS       DASWGG010000327         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2073944, whole genome shotgun sequence.
ACCESSION   DASWGG010000327 DASWGG010000000
VERSION     DASWGG010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2073944"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VF954_05850"
     CDS             <1..998
                     /locus_tag="VF954_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX9530641.1"
                     /translation="SAVTGAGVSQAFAGPDGSSDVAVAGPLPAGGELVVVVPLTASAF
                     QAPLAGARLGATGFAQLVDPAGTVVASTTGSGVGLRDQTGSSPAIVTAIQPLGAGPGS
                     WHVVVAQSRAEIQAETNAAGLGWALLLVVAGLLAGVGTMVATIVRGRARTATAGAEVL
                     GAQAVPAAPDPVPNLRALASPQDLLVATGDALAASAPGDAARVGVVCVRVDGLDHLVG
                     HVGRAVVEDALAIVGRRLQRTVPDPGLVARLADDEFGVLCPAGIASEDVVVVALRVEQ
                     VVQRAIAISGWTLNLSATAGAADGSGADGTSAEALVQQAHQAVRSARCATPPTPS"
     gene            1149..1427
                     /locus_tag="VF954_05855"
     CDS             1149..1427
                     /locus_tag="VF954_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530642.1"
                     /translation="MVPEPVPPAPGAAPSHPLATATRVVYLVAAVVVTVCLVFITFQQ
                     RHQTQLLRRQGCLQQASYIRELEPSTESSQQLLAQARVEVRACVGMKP"
     gene            1603..2592
                     /locus_tag="VF954_05860"
     CDS             1603..2592
                     /locus_tag="VF954_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530643.1"
                     /translation="MVSPGRRRRGPRWLFVGVALTALVLAVNAAISARSPAPARRLSQ
                     LAYLDRVRPLITRSSDEGSRVSDVRSRALALGRAGIVSHLDGVVRDAQSVEASVGTAS
                     PPATLRRAHDLLVAALAIRAKAAVALQTSMTSALATSPVGATVSDLGNAGQDMVAADR
                     AYQLFLDDIPSDVRAEGALPVSQWVTDPATWGRAQLGVFVAALRSSQTLAPVHDVAVV
                     LAEPSPSALGQDGQAQVMATTRSIKVNAVVADTGNEPEKHVTVTASLTGPGNSVESVR
                     DFVDLAAGQSQALTLGDLHPIQGATEVLTVRVVPVDGETDLADNQQVLTLEFR"
BASE COUNT          323 a         1119 c         1033 g          386 t
ORIGIN      
        1 cgtcggccgt gaccggtgcc ggcgtgtcgc aggctttcgc cgggcccgac ggcagctccg
       61 acgtggcggt ggccgggccc ctcccggccg gcggtgagct ggtcgtcgtc gtcccgctca
      121 cggcgtcggc cttccaggcg ccgttggccg gcgcccgtct gggcgccacc ggcttcgccc
      181 agctcgtcga cccggcgggc acggtcgtgg cctccaccac cggcagcggc gtgggcctgc
      241 gcgaccagac ggggtcgtcc ccggcgatcg tgacggccat ccaacccctg ggcgctggac
      301 cggggtcgtg gcacgtcgtg gtcgcccaat cccgggccga gatccaggcg gagacgaacg
      361 ccgccggcct gggttgggcg ctgctgctcg tcgtcgccgg tctgctcgcg ggggtgggaa
      421 cgatggtggc cacgatcgtt cgcggccggg cccgcacggc cacggccggg gccgaggtgc
      481 tcggtgccca agcggtgccg gcggcgcccg atccggtgcc caacctccgc gccctggcct
      541 cgccccagga cctgctggtc gccaccggcg acgcgctcgc cgccagcgcg cccggcgacg
      601 cggcacgcgt cggggtggtc tgcgtccgcg tcgacggcct cgaccacctg gtgggccacg
      661 tcgggcgcgc cgtggtcgag gacgccctcg ccatcgtcgg ccggaggctc cagcgcaccg
      721 tgcccgatcc cggcttggtg gcccggctgg cggacgatga gttcggcgtc ctgtgcccgg
      781 ccggcatcgc gtccgaggat gtcgtcgtgg tcgccctgcg cgtcgagcag gtggtgcaac
      841 gtgcgatcgc catctcgggc tggacgctca acctgagcgc caccgccggc gcagccgacg
      901 gctccggcgc ggacggcacc tccgccgagg cgctcgtcca gcaggcccac caggcggtgc
      961 gctctgccag gtgcgccacg ccccccaccc cgagctgacc cccgtcgcca ccgcggggaa
     1021 gcggcggcag ggagggccga taaagtcgat caatgagcga ctacctgcct cctccccccg
     1081 cgccggagcc ccggcccgag ccatctaaca ctgagccggc gccgcctcac cccgagcccg
     1141 gggttggctt ggtcccggag cccgtaccgc ccgcccccgg ggcggccccg tcccacccgc
     1201 tggcgacggc gacgcgggtc gtctatctgg tggccgcggt ggtggtcaca gtgtgcctgg
     1261 ttttcatcac gttccagcag cggcaccaga cccagctgct gcgccggcag ggctgcctcc
     1321 agcaggcgag ctacatccgg gagctggagc cgtcgaccga gtcgtcccag cagctgctcg
     1381 ctcaggcgag ggtcgaggtc cgggcctgcg tggggatgaa gccctgacgc atcgtcatta
     1441 ggcccggccg gggccccaac cccggcctcc tccgcacggc gggggcccca gccccgccgg
     1501 cggccgttcc catcaggccc ggccggggcc ccaaccccgg cctcctccgc acgccggggc
     1561 cccagccccg ccggcggccg ttcccattag ggtgcggcgg cgatggtgtc tcccggccgc
     1621 cgccggcgcg gcccccgctg gctcttcgta ggcgtggctc tgaccgccct ggtgctcgcc
     1681 gtcaacgcgg ccatctcggc ccgctcgccg gccccggcgc ggcgcctgag ccaactggcc
     1741 tacctggacc gggtccgccc cctgatcacg cgctcgtccg acgagggcag ccgggtctcg
     1801 gatgtccgca gccgggccct ggccctgggg cgggcaggca tcgtcagcca tctcgacggg
     1861 gtggtgcgcg acgcccagtc ggtggaggcg tcggtcggca ccgcctcccc gccggccacg
     1921 ctgcgcaggg cgcacgacct gctcgtggcc gcgctcgcca tccgggccaa agccgccgtc
     1981 gccctccaga cttctatgac ctcggcgctc gccacctcgc cggtgggcgc caccgtgtcc
     2041 gacctgggca acgccggcca ggacatggtc gccgccgacc gggcctacca gctcttcctc
     2101 gacgacattc cctccgacgt ccgggccgag ggcgccctgc ccgtgtcgca gtgggtgacc
     2161 gacccggcga cgtgggggcg ggcgcagctg ggggtgttcg tggcggccct gcggtccagc
     2221 cagacgctgg caccggtcca cgacgttgcc gtcgtgctcg ccgaaccttc gccgtcggcg
     2281 ctgggccagg acggccaggc ccaggtgatg gcgaccacca ggtccatcaa ggtcaacgcc
     2341 gtggtggccg acaccggcaa cgagccggag aagcacgtca ctgtgaccgc ctccctcacc
     2401 gggccgggca acagcgtcga gtcggtgcgg gacttcgtcg acctggccgc gggacagtca
     2461 caggcgctga cgctgggcga cctccacccg atccaggggg cgacggaggt cctcacggtg
     2521 cgggtggtgc ccgtggacgg ggagaccgac ctggcggaca accagcaggt gctgacgctc
     2581 gagttccggt gattctcagc gccgaacgca aagctacttg ggcggccgga ggccatcttc
     2641 ccggtcgcga cgccccggtc taagggagcc cgcccaagta gttttgcgct tggcactcag
     2701 ctggcgtcgt cgtcctcggg gccccggtcg cgccgccact tcagctcggc cagggtcacg
     2761 acctcgcgga gcgggcgccc ggtgcggcgc gccacccggg cggcgtcgtc gtgctcggcc
     2821 ttgacccggc ccgggctgac cttgacccgc accggcagcc c
//
LOCUS       DASWGG010000328         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_461084, whole genome shotgun sequence.
ACCESSION   DASWGG010000328 DASWGG010000000
VERSION     DASWGG010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461084"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VF954_05865"
     CDS             <1..507
                     /locus_tag="VF954_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX9530644.1"
                     /translation="DCVLSHLMVDHAGGVGCLAPGTRVWVQRAEWDYAHGPSPDRLAY
                     RPADLPPLSAGELDVRLIEGDAAPWPGIRLLLTPGHCPGHMSMLVELPDGWIALAADA
                     ADTQRNLTERIAPGILLAGRDQALASLDRFREEATALGALMIPGHDPEVWDLIPRSFG
                     SSATERVP"
     gene            504..806
                     /locus_tag="VF954_05870"
     CDS             504..806
                     /locus_tag="VF954_05870"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX9530645.1"
                     /translation="MRLSPTDVDKLLLSVAAMVARDRRARGVKLNVPEATALLSSFVL
                     EGARDGRSVADLMAAGRAVLSRDDVMEGVPELLHEVQVEATFPDGTKLVTLHQPIP"
     gene            913..1203
                     /locus_tag="VF954_05875"
     CDS             913..1203
                     /locus_tag="VF954_05875"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEX9530646.1"
                     /translation="MIPGEVVAADGTIELNPGRERRRVLVENTGDRPVQVGSHFHFAD
                     VNSALAFDRAAAQGFRLDVPAGTGVRFEPGMSKEVGLVALAGRRVVPGLQRR"
     gene            1217..>2234
                     /locus_tag="VF954_05880"
     CDS             1217..>2234
                     /locus_tag="VF954_05880"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX9530647.1"
                     /translation="MNIGRRRYAELYGPTVGDKVRLGDTDLWISPTEDRCSPSGAGDE
                     TVFGGGKVIRESMGQATFSTVDLVITNAVVLDHWGVVKADVGVRDGRITAIGKAGNPD
                     IMDGVDPDLVVGPGTEVLAGEGRILTAGIVDCHVHLICPQVIEVAMAAGTTTIIGGGT
                     GPAEGTRATTVTPGAWNLALMLKALDRWPVNVALLGKGNTVSQEALEAQARAGASGYK
                     LHEDWGTTPAAIDACLTACDRLGVQVAIHTDTLNEAGFVADTLAAIDGRSIHAYHTEG
                     AGGGHAPDIITVASAPNVLPSSTNPTRPHTTNTIDEHLDMLMVCHHLNPRVAEDLAFA
                     ESRIR"
BASE COUNT          330 a          839 c          778 g          287 t
ORIGIN      
        1 gactgcgtgc tcagccacct catggtggac cacgccggcg gcgtggggtg cctggcgccc
       61 ggcacgaggg tctgggtcca gcgggccgag tgggactacg cgcacggccc ctcgcccgac
      121 aggctggcct accgcccggc ggacctccca ccgctgtccg ccggagaact cgacgtccgc
      181 ctgatcgagg gcgacgcagc cccgtggccc ggcatccggc tgctgctgac gccgggccac
      241 tgcccggggc acatgtcgat gctcgtcgag ttgcccgacg gctggatcgc cctcgccgcc
      301 gacgccgccg acacgcagcg caacctcacc gagcgcatcg cgcccgggat cctgctcgcc
      361 gggcgggacc aggcccttgc ctcgctcgac cgcttcaggg aggaggcgac ggccctcggc
      421 gccctgatga tcccgggcca cgaccccgag gtctgggacc tgatcccgcg gtcgttcggc
      481 tcgagtgcca ccgagcgcgt gccgtgaggc tgtcccccac cgacgtcgac aagctgctcc
      541 tctcggtggc ggcgatggtc gcccgggacc ggagggccag aggggtgaag ctcaacgtcc
      601 ccgaggcgac ggccctgctc tcctccttcg tgctggaggg cgcccgggac ggccgctcgg
      661 tggccgacct catggccgcg ggccgggcgg tgctgagccg ggacgacgtg atggaggggg
      721 tgcccgaact cctccacgag gtccaggtcg aggcgacctt ccccgacggc acgaagctcg
      781 tgaccctcca ccagccgatc ccatgacgaa cgcccagctc caggccgcag gcccctggag
      841 acgaactttg agtacaaact gttgctctac gccggttttt actcaaagaa cgtcacgacc
      901 cggggttacc cgatgatccc cggcgaggtc gtcgcggcag acggcacgat cgagctcaac
      961 ccgggccggg agcggcgccg ggtgttggtc gagaacaccg gcgaccggcc ggtccaggtc
     1021 ggctcgcact tccacttcgc cgacgtgaac tcggccctcg ccttcgaccg cgccgcggcg
     1081 caggggttcc gcctcgacgt ccccgccggc accggcgtgc gcttcgagcc gggcatgtcg
     1141 aaggaggtcg gcctggtcgc cctggcggga cgccgcgtgg tccccgggct gcagcggcga
     1201 tgaacgccgg gcggcgatga acatcgggcg ccggcgctat gccgagctgt acggccccac
     1261 cgtgggcgac aaggtccgcc tcggcgacac cgacctgtgg atctcgccga ccgaggaccg
     1321 gtgctcgccg tccggggctg gcgacgagac ggtcttcggg ggcggcaagg tcatccggga
     1381 gtcgatgggc caggcgacgt tctcgaccgt cgacctcgtc atcaccaacg ccgtcgtgct
     1441 cgaccactgg ggcgtggtga aggccgacgt cggggtgcgg gacggccgca tcaccgccat
     1501 cggcaaggcg gggaaccccg acatcatgga cggcgtcgac ccggacctcg tcgtcggccc
     1561 cggcaccgag gtgctagcgg gcgagggccg catcctgacc gcggggatcg tcgactgcca
     1621 cgtccacctg atctgcccgc aggtcatcga ggtggcgatg gcggcgggga ccacgacgat
     1681 catcgggggc ggcaccgggc cggccgaggg gacccgggca acgaccgtca cgccgggagc
     1741 ctggaacctc gcgctcatgc tgaaggccct ggaccgctgg cccgtcaacg tcgccctgct
     1801 gggcaagggc aacaccgtgt cgcaggaggc gctggaggcg caggcccgag ccggggcgtc
     1861 gggctacaag ctccacgagg actgggggac gacccccgcc gccatcgacg cctgcctgac
     1921 cgcgtgcgac cgcctcggcg tccaggtcgc catccacacc gacacgctga acgaggcggg
     1981 gttcgtcgcc gacacgctcg ccgccatcga cggcaggtcg atccacgcct accacaccga
     2041 gggcgccggc gggggccacg cccccgacat catcacggtc gcctcggccc ccaacgtcct
     2101 gccgtcctcg accaacccga cccgcccgca caccaccaac accatcgacg agcacctcga
     2161 catgctgatg gtctgccacc acctgaaccc gagggtcgcc gaggacctcg ccttcgccga
     2221 gagccgcatc cggc
//
LOCUS       DASWGG010000329         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1908578, whole genome shotgun sequence.
ACCESSION   DASWGG010000329 DASWGG010000000
VERSION     DASWGG010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908578"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VF954_05885"
     CDS             <1..210
                     /locus_tag="VF954_05885"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX9530648.1"
                     /translation="YPPNFKLRLAVKDMDLVHDTAARLGLELRVAESARSWFKDAAAR
                     GLGEMDYSAVVALITGAPAELPPPE"
     gene            324..1501
                     /locus_tag="VF954_05890"
                     /pseudo
     CDS             324..1501
                     /locus_tag="VF954_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          215 a          569 c          503 g          214 t
ORIGIN      
        1 tacccgccga acttcaagct ccggctggcc gtgaaggaca tggacctcgt ccacgacacg
       61 gcggcccgcc tgggcctcga gctgcgggtt gccgagtccg cgcggtcctg gttcaaggac
      121 gcagcggcca ggggcctcgg cgagatggac tactcggcgg tcgtcgccct catcaccggc
      181 gcgcccgccg aactgccgcc gcccgaatag ccgcccgctt gccgtagccc tgtccgcggc
      241 actaccgtac gccggggtcc ccttggggca acaatgggac cttcaacgac aggaggccct
      301 acatggcgga cgaggtagtg gagatgctgc gcgaggcggc ccgggaggcg cttcctccgg
      361 tggaagggga gctccaggtc ggcggcctga ccggcccagt cgaggtccgc cgggaccgct
      421 ggggggtccc gcacatcggc gcctccaacc tccacgacct cttcctcgcc cagggcttcg
      481 tcgccgcctc cgaccggtac ttccagatcg agctgctgat gcgctactcc tagggccgcc
      541 tctcggagct catcggggcc ctcacgctgc cgctggaccg cttcgtccgg acgctcggct
      601 ggcccgcgat ggccgcccgc cacgtcgccg ggtgggacca ggagtcgaag gaaaacgtcg
      661 gcgccttctg gtcgggggtt gccgcctggg gcagccagat ggcggcgccg ccggtcgagt
      721 acgcgatcct cgaggcggac ccctggatcc cgacggtgga gcagggctgc ctggcgaccg
      781 ccggcttcgc cctgctcctc ggctacacgc tcacgcgcaa ctgggacgcc gaactcctgc
      841 gggccgaggt cgccgagcgc ctcggggtcg acgccatgct cgacctcttc ccggacgtct
      901 cgaacctccc cgcgttcatc caggcgggca agagcacgca tcccgctcgc agcgcgctcc
      961 tgacccacgc ccacctgccg acctccgggc agggctcgaa cgagtgggtg gtggccgggg
     1021 agcgcagcgc caccggcaag ccgctgctcg ccaacgaccc gcacctggcg atcctgctgc
     1081 cggccatgtg gtacgagtgc gacctggtgg cccccggctt ccatgccgcg ggggcatcgc
     1141 ttcccttcgc cccgggcatc gtcatcggcc acaacgagcg catcgcctgg ggcgtcaccg
     1201 acaccgagtc cgacgtcgcc gacctctacc tggaacgcct gtccggtgac gggacgtcgg
     1261 tggagtacaa ggggggctgg gagccactca ggatccggac cgagaccatc acgatccgcg
     1321 acggcgacga cgtcgtcctc gaggtcaagg agacccggca cggcccgctg ctcgactcgt
     1381 acctgatcgg catcgccgac cctgcctcca tcgagggcgg gatcacgaag tcctacgccc
     1441 tgcgctggac ggcggcccgg gcgtcggtcc agccctccaa cctcacccgc atcaacgccg
     1501 c
//
LOCUS       DASWGG010000330         4537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1584868, whole genome shotgun sequence.
ACCESSION   DASWGG010000330 DASWGG010000000
VERSION     DASWGG010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584868"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VF954_05895"
     CDS             complement(<1..467)
                     /locus_tag="VF954_05895"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HEX9530649.1"
                     /translation="MSMGRTVRRSRDGRYHLRLSDEERALLAGLGDDLLADLEDDDDE
                     GIQRLFPTAHHDDPVGDAEYHLRHHDELLTGRRATIAAFVQTAESDTLGEEQLQIWMT
                     VINDIRLVVGTHLDVSEDMDDIVAPDPRAPQYALYMYLGGLLSDLIDAAFGGLP"
     gene            complement(519..824)
                     /gene="clpS"
                     /locus_tag="VF954_05900"
     CDS             complement(519..824)
                     /gene="clpS"
                     /locus_tag="VF954_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX9530650.1"
                     /translation="MATAPPDLAPTGVELPIDQDVTTPDLPWIVIVWNDPINLTSYVT
                     YVFQKLFGYSKEKATRLMLEVHHKGRAVVSNGTREEAERDVARLHAHGLWATMERES"
     gene            complement(837..1796)
                     /locus_tag="VF954_05905"
     CDS             complement(837..1796)
                     /locus_tag="VF954_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX9530651.1"
                     /translation="MSPPLRRIWLRVPRISPETYRGITLLAAIALGFIIVTGGAVRLT
                     GSGLGCPDWPTCARHHVVAPVEYHAMVEFVNRVVTGAVSVLVVVAVLGALVREPRRRD
                     LTWLAGGLVAGVVGQIVLGGLAVLEKLAPPFVMAHFLLSLALLCDAVVLHYRAGETVG
                     PARLLVDRWHLALSRALVVLTTVAVFLGTVVTSTGPHGGDPKAPRFHFSLHRVAQLHG
                     GAVTALLALTLYLAWSLRRAGAPAAVQARLRALVQIMVLQAGVGYLQYFTGVPVFLVG
                     IHVAGAASVWAGVVWFHAGLASRSPSGAPATVTVSVQPALAGT"
     gene            complement(1809..2039)
                     /locus_tag="VF954_05910"
     CDS             complement(1809..2039)
                     /locus_tag="VF954_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX9530652.1"
                     /translation="MAVSHRLRVDPIACDGRGLCADLFPERVRLDDWGFPIIDPAPVG
                     GDLVEHARRAVAACPKLALILERQGRSARQRA"
     gene            complement(2030..3364)
                     /locus_tag="VF954_05915"
     CDS             complement(2030..3364)
                     /locus_tag="VF954_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX9530653.1"
                     /translation="MTPGAALAAQAETVPGDAPAGRARLLADLPAAGTWRSLAEHRRR
                     YPPPPAISGRARRAVIAEVERAGLRGRGGAGFPTAVKLAAVAKGRGRSYVVANGAEGE
                     PASGKDKLLLTAAPHLVLDGAQWAAAAVGASEIVVAVDRRATGALAHVERALAERAGG
                     PGAVPIRLVATPPRYIAGEESALVNWLNGGPAKPTLVPPRPFERGMNGRPTLVDNVET
                     LAHLAQICRWGAPWFRGLGTDEEPGTALLTISGGVERPGVYEAALGTPVGQVLSAAGA
                     GPASAVLVGGYFGTWIPGPSAVTTPVSRAGLQPLGAAVGCGVLAVLPEGLCGVHDSAR
                     VLNWLAAETAGQCGPCVHGLAAIAGTLSQLYHGRGGLAARDRLHRWAGMVEGRGACRH
                     PDGAVRFLRSALSTFDHELAVHATTGGCSFGPLGGQRPVLPLPRAEEVPEWR"
     gene            complement(3361..3954)
                     /locus_tag="VF954_05920"
     CDS             complement(3361..3954)
                     /locus_tag="VF954_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX9530654.1"
                     /translation="MIPVAASVAPKALWYLTRATGVVCLVLLTASVVLGITTSVGWSA
                     PRWPRYLLEGLHRNVSLLVVVFLGVHILTTVVDGYAPIGWLDAVVPFRSPYRSLWLGL
                     GAVACDLLLAVAVTSLVRVRLGYRVWRGIHWLAYACWPVALAHGLGTGSDTRQSWMLL
                     LDAAAVVAVGAAIWWRVALAWPAPAAVRDATSAGSRR"
     gene            complement(3951..>4537)
                     /locus_tag="VF954_05925"
     CDS             complement(3951..>4537)
                     /locus_tag="VF954_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX9530655.1"
                     /translation="PVARVAPVAGWKVVEVDRHRSTIRVPAGVALDLGATAKAWAADQ
                     ASAAAADAAGCGVLVSLGGDVAVAGPVPGRGWPVRVAEAHDAPAEAPGQTVNLTSGGL
                     ATSSTTVRRWQRAGVEQHHLVDPATGRAAAGYWRTVTVAAGSCADANVAATAAIIRGE
                     GAPRWLEQLGLPARLVRRDGQLAHAAAWPAEVPA"
BASE COUNT          623 a         1663 c         1731 g          520 t
ORIGIN      
        1 ggcaggcccc cgaacgcggc gtcgatcaga tcggacagca gcccgcccag gtacatgtac
       61 agcgcgtact ggggcgcccg cgggtcgggg gccacgatgt cgtccatgtc ctcgctcacg
      121 tcgaggtgcg tgccgaccac gaggcggatg tcgttgatga cggtcatcca gatctggagc
      181 tgctcctcgc cgagggtgtc ggactcggcc gtctggacga aggcggcgat ggtggccctg
      241 cgcccggtca gcagctcgtc gtggtggcgg aggtggtact cggcgtcgcc caccgggtcg
      301 tcgtggtggg cggtcgggaa gaggcgctgg atgccctcgt cgtcgtcgtc ctccaggtcg
      361 gccaggaggt cgtccccgag gccggcgagc agcgcccgct cctcgtcgga gagccggagg
      421 tggtagcggc cgtcgcggga gcgccgaact gtccggccca tgctcatgcc ggcgccatgc
      481 tcattgccgg cgccatgttc atgccggtac cacgcggctc acgactcgcg ctccatggtg
      541 gcccacagcc cgtgggcgtg caggcgggcg acgtctcgct cggcctcttc cctcgtgccg
      601 ttggacacga cggcccggcc cttgtggtgc acctccagca tcagcctggt ggccttctcc
      661 ttgctgtagc cgaacagctt ctggaacacg taggtcacgt acgacgtgag gttgatgggg
      721 tcgttccaca cgatgacgat ccacggcagg tccggcgtgg tgacgtcctg gtcgatcggt
      781 agctcgactc cggtcggggc cagatcggga ggggcggtgg ccatctgttg cgggcctcac
      841 gtgcccgcga gcgcgggctg gacggagacg gtgacggtgg ccggtgcccc cgaaggggac
      901 cggctcgcca ggccggcgtg gaaccacaca actcccgccc ataccgacgc ggccccggcg
      961 acgtggatcc cgacgaggaa caccgggacg ccggtgaagt actggaggta gccgacgccg
     1021 gcctgaagga ccatgatctg cacgagcgcc cggagccggg cctgcacggc ggccggggcg
     1081 ccggcccggc gcagcgacca cgccaggtac agggtcaggg cgagcagggc ggtgaccgcg
     1141 ccgccgtgca gctgggcgac acggtggagg gagaagtgga agcggggtgc cttggggtcg
     1201 ccgccgtggg gaccggtgga ggtgacgacg gtcccgagaa agacggccac ggtggtgagc
     1261 acgaccagcg cccggctcag cgccaggtgc caccggtcca ccagcagccg tgccgggccc
     1321 accgtctcgc cggcccgata gtgcaggacc acggcgtcgc acagcagcgc gagcgagagc
     1381 aggaaatggg ccatgacgaa cgggggcgcc agcttctcca gcacggccag cccccccagc
     1441 acgatctggc ccacgacgcc cgccaccaac ccaccggcca gccacgtcag gtcccgccgg
     1501 cggggctccc gtaccagcgc gcctagaacg gctaccacga cgagcacgga cacggccccg
     1561 gtgaccaccc ggttcacgaa ctcgaccatg gcgtggtact ccaccggcgc gacgacgtga
     1621 tggcgcgcgc acgtcggcca gtcggggcag cccagcccgg acccggtgag gcgcacggcg
     1681 ccgcccgtga cgatgatgaa accgagggcg atcgcggcca gcagggtgat gccgcggtag
     1741 gtctcgggac tgatccgggg cacccgcagc cagatgcggc ggaggggcgg ggacatcggg
     1801 cttcgatgct aggcgcgttg gcgggccgag cggccctgcc tctccaggat cagggccagc
     1861 ttcgggcacg ccgccaccgc ccgccgggcg tgttccacga gatccccgcc gactggcgcg
     1921 gggtcgatga tcggaaagcc ccagtcgtcc aggcggacgc gttcggggaa gaggtcggcg
     1981 cacaggcccc gcccgtcgca cgcgatggga tcgacgcgca gccggtggct caccgccatt
     2041 ccggcacctc ctcggccctc gggaggggaa gcacggggcg ctgcccgccg aggggcccga
     2101 acgagcaccc gccggtggtg gcgtggaccg ccagctcgtg gtcgaaggtg gacagcgcgc
     2161 tgcgcaggaa gcgcaccgcc ccgtccgggt ggcggcacgc ccctcggccc tcgaccatgc
     2221 cggcccagcg gtggagccgg tccctcgcag ccagcccgcc gcgcccgtgg tacagctggg
     2281 aaagggtgcc cgcgatggcg gccagcccgt ggacgcacgg accgcactgg ccggcggtct
     2341 ccgcggccag ccagttgagg acccgggccg agtcgtgcac gccgcacagg ccttcgggca
     2401 ggacggcgag gacgccgcat ccgaccgccg cgcccagggg ctggagcccg gccctgctga
     2461 cgggggtggt gacggcggac gggcccggga tccaggtgcc gaagtagccg cccaccagca
     2521 cggccgacgc cgggccggcg ccggccgccg acaggacctg gcccaccggc gtgcccaggg
     2581 ccgcttcgta aacgcccggg cgctcgacac cgcccgagat ggtgagcagc gccgtgccgg
     2641 gctcctcgtc ggtgcccagg ccacggaacc agggcgcgcc ccaccggcag atctgggcca
     2701 ggtgggccag ggtctccacg ttgtcgacca gggtgggccg gccgttcatg cctcgctcga
     2761 acgggcgcgg tgggacgagg gtcggcttgg ccggtccgcc gttgagccag ttgacgaggg
     2821 cgctctcctc accggcgatg tagcgcgggg gcgtggccac caggcggatc gggaccgcac
     2881 ccgggccgcc ggcgcgttcg gcgagggcgc gctccacgtg ggcgagggcg ccggtcgccc
     2941 ggcgatcgac tgccaccacg atctcgctgg cgcccacggc ggccgcggcc cactgggccc
     3001 cgtcgaggac caggtgcggc gccgcggtca gcagcagctt gtccttgccg ctggccggct
     3061 cgccctcggc gccgttggcg accacgtacg accggccgcg gcccttggcc accgcggcca
     3121 gctttacggc ggtcgggaag ccggcgccgc cgcggcctct gaggccggcg cgctcgacct
     3181 cggcgatgac ggcccggcgg gcccgcccgg atatggccgg cggtggcggg taccgccggc
     3241 ggtgctcggc gagggaccgc cacgtgccag ccgccggcag gtcggccagc aggcgggccc
     3301 ggccggccgg cgcgtcgccg ggcacggtct cggcctgagc tgccagcgcc gcgccggggg
     3361 tcatcgtctg ctccccgcgc tggtcgcgtc gcggacggcg gccggggcgg gccacgccaa
     3421 tgccacccgc caccagatgg cggcgccgac tgcgaccacg gcggccgcgt cgagcagcag
     3481 catccagctc tgccgggtgt cgctgcccgt cccgaggccg tgcgccaggg cgaccggcca
     3541 gcacgcgtac gccaaccagt ggatgccccg ccagacccgg tagccgagcc ggacgcggac
     3601 caggctcgtg acggctaccg cgagcaggag gtcgcaggcg acggcgccga ggccgagcca
     3661 gaggctccgg tagggggagc ggaagggcac gacggcgtcg agccagccga tgggggcgta
     3721 gccgtcgacc acggtggtga ggatgtggac gccgaggaag acgacgacga gcagcgacac
     3781 gttcctgtgc aggccttcca gcaggtagcg cggccatcgc ggcgccgacc agccgaccga
     3841 cgtcgtgatg cccagcacga cggacgcggt gagcagcacc aggcacacca cgcctgtggc
     3901 ccgggtgagg taccacaggg ccttcggcgc gaccgaggcg gctaccggga tcacgcgggt
     3961 acctccgccg gccaggccgc ggcatgggcc agctgcccgt cgcggcgcac gaggcgagct
     4021 ggcagcccga gctgctccag ccagcgcggc gcgccctcgc cgcggatgat cgccgccgtg
     4081 gcggccacgt tggcgtcggc gcagcttccg gccgcgacgg tgaccgtccg ccagtagccc
     4141 gccgccgccc gcccggtcgc cgggtcgacc aggtggtgct gctccacgcc ggcgcgttgc
     4201 caccgccgca ccgtcgtgct cgacgtggcc aggccgccgg aggtgaggtt gaccgtctgg
     4261 cccggagctt cggccggggc gtcatgggct tcggccacgc gcaccggcca gcccctgccc
     4321 ggaacgggac cggccacggc cacgtcgccg cccaggctga ccaggacgcc gcagccggcc
     4381 gcgtccgccg ccgcagccga ggcctggtcg gcggcccagg ccttggcggt ggcgcccagg
     4441 tcgagggcca cgccggcggg cacccggatc gtcgagcggt gccggtccac ctccaccacc
     4501 ttccagcccg ccacaggcgc gacgcgcgcg accggcc
//
LOCUS       DASWGG010000331         2589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_940414, whole genome shotgun sequence.
ACCESSION   DASWGG010000331 DASWGG010000000
VERSION     DASWGG010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940414"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /gene="dapB"
                     /locus_tag="VF954_05930"
     CDS             complement(<1..549)
                     /gene="dapB"
                     /locus_tag="VF954_05930"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX9530656.1"
                     /translation="MGVFGAGGRMGSTVCRAVVADRDLELVAAVDPHHSGLDLRQVAG
                     VEGTGLQVGAGPEALAAAGAEAAVDFTEARAAWDNLLWCADHGVHAVVGTTGLSDEQL
                     GTLAGRFDAGGANCVVAPNFAIGAVLMIRLAELASPFFDSAEIIELHHDGKTDAPSGT
                     ALHTATRMSGTSKQWSPDPTRTE"
     gene            complement(565..1815)
                     /locus_tag="VF954_05935"
     CDS             complement(565..1815)
                     /locus_tag="VF954_05935"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9530657.1"
                     /translation="MISTSRLDSGLVVVTECMPDVRSVTAGFWVGTGSRDEEPHQAGV
                     SHFLEHLLFKGTSRRTARQIAEDVDSVGGDMNAFTTKEYTAFYVRVLADALPLGLDIL
                     SDIMWDPAFQASEVEAERQVILEEILMHNDEPADLVHEVFAAALYPGHPLGRDVLGTE
                     ATIEPMSAAEIRRFFAGHYRPANMVLAAAGDLDHDDFVAAVESRFQGPEGGRAPARRP
                     PVAVPRDMAVVRRMTEQAHVVLGAPGPDRHSDDRFAFTILNHCVGGGLSSRLFQAIRE
                     ERGLAYSVYSYRSSFADAGNIGVYAGTAPGRAAEVIDVMTEEVDRLVADGISDRELAV
                     AKGHLRGETALALEDSAARMSRIGRSQLLHGSVLTVEELAARFAAVTADEVGDVARRT
                     LGDRRVLAVVGPFDEDAFGASVVA"
     gene            complement(1834..>2589)
                     /locus_tag="VF954_05940"
     CDS             complement(1834..>2589)
                     /locus_tag="VF954_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX9530658.1"
                     /translation="ARLKILDVMNGAIAAPRSEVRDTAPKIVSFEIPMDKIGEVIGPK
                     GKVINTIQQETGADVNVDDDGVIGTVTIGSKDGAAVTEARRRIELILDPPKAEVGATY
                     PGRVVNITKFGAFVNILPGRDGLVHISKLGRGRRVERVEDVVDLGDELLVRVDDIDPQ
                     GKVSLSLVGDGDEAGSGAAPSAAVRDAAGPSGPANRGGREREAPRAVSFEDSFEKELQ
                     GEFGDLGPGEQQAAARHGERVGDRGPRRNPRRR"
BASE COUNT          350 a          959 c          911 g          369 t
ORIGIN      
        1 ctccgtgcgc gtcgggtccg gactccattg cttggacgtt cccgacatcc gcgtcgcggt
       61 gtgtagcgct gtcccggacg gcgcatcggt cttgccgtca tggtgcagct cgatgatctc
      121 ggccgagtcg aagaatggcg acgccagctc ggccagccgg atcatgagca ccgcgccgat
      181 ggcgaagttg ggggccacga cgcagttggc gcccccagcg tcgaaacggc cggcgagggt
      241 gccgagctgc tcgtcgctca ggccggtcgt gccgaccact gcgtggacgc cgtggtccgc
      301 gcaccacagc aggttgtccc acgcggcgcg ggcttcggtg aagtcgaccg ccgcctcggc
      361 gccggccgcg gccagtgcct cgggtccggc tcccacctgc aggcccgtcc cctcgacgcc
      421 cgccacctgg cggaggtcca gccccgagtg gtgaggatcg accgcggcca ccagctcgag
      481 gtcccggtcg gccacaaccg cccggcagac ggtggagccc atgcggccgc cggcgccgaa
      541 cactcccacc cgcacccggg ccgcctacgc caccacggac gcgccgaagg cgtcctcgtc
      601 gaacggcccg accacggcga gcacccgccg gtccccgagg gtcctccggg ccacgtcgcc
      661 gacctcgtcg gcggtgacgg cggcgaagcg ggcggcgagc tcctcgacgg tcagcaccga
      721 cccgtggagg agctggctgc gcccgatccg gctcatgcgg gcggccgagt cctcgagggc
      781 cagggcggtc tcgccccgca ggtggccctt ggccacggcg agctcccgat cggagatccc
      841 gtcggccacc agccggtcga cctcctccgt catgacgtcg atgacctcgg ccgcccgccc
      901 gggcgcagtg cccgcgtaca ccccgatgtt gccggcgtcg gcgaaggagc tgcggtagga
      961 gtacaccgag taggccaggc ccctctcctc ccggatggcc tggaacaggc ggctcgacag
     1021 gcccccgccg acgcagtggt tgaggatggt gaaggcgaag cggtcgtcgc tgtggcggtc
     1081 ggggccgggc gcgccgagca ccacgtgggc ctgctcggtc atgcgacgca cgacagccat
     1141 gtcacgggga acggccacgg gaggccggcg ggccggcgcc cgcccaccct ccggtccctg
     1201 gaagcgggac tcgacggccg ccacgaaatc gtcgtggtcg agatccccgg ccgcggccag
     1261 caccatgttg gccggccggt agtggccggc gaagaaccgc ctgatctcgg ctgccgacat
     1321 gggctcgatc gtggcctcgg tgccgagcac gtcccggccg agcgggtgcc cggggtacag
     1381 ggcggccgcg aagacctcgt gcaccaggtc ggccggctcg tcgttgtgca tcaggatctc
     1441 ttccaggatg acctggcgct cggcctccac ctcgctcgcc tggaaggcgg gatcccacat
     1501 gatgtcggac aggatgtcga gcccgagggg cagggcgtcg gccagcacgc gcacgtagaa
     1561 cgccgtgtac tccttggtgg tgaaggcgtt catgtcgccc ccgacgctgt cgacgtcctc
     1621 ggcgatctgg cgcgccgtgc gccggctggt ccccttgaag agcaggtgct ccaggaaatg
     1681 cgagacgccc gcctggtggg gctcctcgtc ccgcgagccc gtgccgaccc agaagccggc
     1741 cgtcacggac cgcacgtccg gcatgcactc ggtgacgacc acgaggccgg aatcgagccg
     1801 gctggtgctg atcaacgccc ggtcatggcc cgatcagcgc cggcgggggt tgcgccgggg
     1861 gcctcggtcg cctacgcgct cgccgtggcg ggcagccgcc tgctgctcac caggcccgag
     1921 gtcgccgaac tcaccctgca gctccttctc gaagctgtcc tcgaaggaca cggccctggg
     1981 cgcctcccgc tcgcgcccgc ctcggttggc ggggcccgag gggccggcgg catcccggac
     2041 cgcggcgctc ggcgccgcgc cggagccggc ctcgtcaccg tcgccgacaa gcgagaggga
     2101 gaccttgcct tgggggtcga tgtcgtcgac ccgcaccagc agctcgtcgc ccaggtcgac
     2161 cacgtcctcg acccgctcga cgcgcctgcc ccggcccagc ttggagatgt gcaccaggcc
     2221 gtcccggccg gggaggatgt tgacgaaagc gccgaacttg gtgatgttca ccacccgccc
     2281 cgggtaggtc gcgccgacct cggccttcgg cgggtccagg atcagctcga tccgccggcg
     2341 ggcctcggtg accgccgccc cgtccttgga gccgatggtg accgtgccga tcaccccgtc
     2401 gtcgtcgacg ttgacgtcag cacccgtctc ctgctggatg gtgttgatca ccttgccctt
     2461 gggcccgatg acctcgccga tcttgtccat cgggatctcg aagctcacga tcttcggcgc
     2521 ggtgtcgcgc acctcggaac gaggagccgc gatcgcgccg ttcatgacgt cgaggatctt
     2581 caagcgggc
//
LOCUS       DASWGG010000332         6719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3201052, whole genome shotgun sequence.
ACCESSION   DASWGG010000332 DASWGG010000000
VERSION     DASWGG010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3201052"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VF954_05945"
     CDS             complement(<1..929)
                     /locus_tag="VF954_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530659.1"
                     /translation="MTFGAFSGERRRGAAIMAAAALGLSLSSCVAGRASLIKAPPPLP
                     PNTSTTAAPDLSGVSLAGAPGRTTTTLAVGPGAASLSGTVSGPNGPIPAPAVIVHVER
                     LVGNAEATQDVATQPDGTWSLPGILGGRYRVRAYRPPDFAELDAQVFWLGGAEARQLA
                     LKVDQFSGVNVAAALAPSPAVVGEPAQLVVQVTTRSVDPQGIVRAVPVVGTSVALTGP
                     GSWTIAANPATTTGNGRVAYAVVCQQLGDQPLSAVVGTSDTFPLNLPPCVPEPPATTE
                     PPTTAVSSSTTTPVIGPPGSTSTTPARSTTTTAG"
     gene            complement(1010..1900)
                     /locus_tag="VF954_05950"
     CDS             complement(1010..1900)
                     /locus_tag="VF954_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530660.1"
                     /translation="MGTIWAWWPRDVLLVSGPEAVSFLQGQVSQDVAAVGVGESKLSF
                     VLQPQGKVDALVRLTRRAEDAVLVDVDGGFGPAVAERLGRFKLRVKAEIVALPWRCLA
                     VRGPGAGAVASGGPGEDGVVVPALWPGVEGVDVLGPAPAVPPGTPIVAADELEWLRVR
                     AGMPAMGRELTERTIPAETGLVGAAVSFTKGCFTGQELVARIDSRGGHVPRNLRSVVI
                     PGERVPPAGAAVVVDGRPVGEITSAARGSWTCAPGEVGGEVVALAYVRREVEVPVAAS
                     VEWDGLGVEAGVAALPGATA"
     gene            complement(1934..3406)
                     /locus_tag="VF954_05955"
     CDS             complement(1934..3406)
                     /locus_tag="VF954_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX9530661.1"
                     /translation="MVPVWREVLADLSTPVAAFLRVVGDEPGFLLESVEGGERWSRFS
                     FVGRRPLATIVARAGHPLDVQGRLPVDVPTDRGVLAATEAILAGCRSPAIAGLPPLHG
                     GLVGYLGYDVVREVERLPNVPRDDKGHPDAVLSVIGQLAAFDHWRQRVQLIDNVMVDP
                     AWRPADLDAAYDAAMARVDALAADGARFLDEPVVEPPPQDEVPPDVTRGHSLAEWSRA
                     VEVAKDHIVAGDIFQVVLSQRFDLDLGADPFDLYRALRQVNPSPYMYFVRDPRLCIVG
                     SSPEPMVQLLDGTVVSRPIAGTRRRGRTEADDRRLAGELSEHPKEVAEHIMLVDLARN
                     DVGRVVDFGTEQVDELMTLERYSHVMHLTSQVSGRLRAGLGPIDVLRATLPAGTVSGA
                     PKVRAMEIIDDLEPTKRGPYAGVVGYIDFSGNLDTAIAIRTMVVTPDGRASVQAGAGI
                     VADSDAANEDLECRNKAAGLLAAVPTARRMSAARASHAPR"
     gene            complement(3476..3853)
                     /gene="hisI"
                     /locus_tag="VF954_05960"
     CDS             complement(3476..3853)
                     /gene="hisI"
                     /locus_tag="VF954_05960"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX9530662.1"
                     /translation="MSAVTPIEVAEGELDSITYDAAGLVPAIAQERSTGKVLMMAWMT
                     AGTLRETLSSGRMVYWSRSRAERWAKGDTSGDRQWVREAYYDCDGDVLLFLVDQEGGG
                     ACHTGNHSCFYRAFGSGPGAPRP"
     gene            complement(3850..4179)
                     /locus_tag="VF954_05965"
     CDS             complement(3850..4179)
                     /locus_tag="VF954_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX9530663.1"
                     /translation="MVSAKEHKQPITMLADRVLVQIPQSEGERKSRSGILIPATAQVS
                     RRLAWAEVVAVGPHVRNITPGDQVLFNPEDRYEVEVQGDDYIILRERDIHAVASERID
                     GGTGLYL"
     gene            4201..4827
                     /locus_tag="VF954_05970"
     CDS             4201..4827
                     /locus_tag="VF954_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530664.1"
                     /translation="MVIEPIRLHTSDELALEAELSVPPDPRAAVVLAHPHPRQGGNMR
                     SIVPGALFAALPGLGVAALRFNFRGVEGSEGSYGGGIGERHDIVAAVGALHDVTEGLP
                     LVLAGWSFGADTSLAVVDERITAWMAIAPPLRTAPIEDLVASADPRAKLLVVGDHDQY
                     RPPEQARPLVAAWVNTELEVVAGADHFFVGRTDRLVELAVAYVDRLTR"
     gene            complement(4848..5648)
                     /gene="hisF"
                     /locus_tag="VF954_05975"
     CDS             complement(4848..5648)
                     /gene="hisF"
                     /locus_tag="VF954_05975"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX9530665.1"
                     /translation="MRSVRVIPCLDVDGGRVVKGVNFQNLRDAGDPAELAARYAAEGA
                     DEIVFLDITASSDDRATIVDVVRRTAEQVFIPLTVGGGVRSVEDGRRLLRAGAEKVGV
                     NTAAVARPAVVAELAREYGTQCVVVAIDARACGGGRWEVFTHGGRRPTGIDAVSWAVV
                     CERLGAGEILLTSMDRDGTGDGFDLALTRAVVEATNVPVIASGGVGTLDHLVEGAVAG
                     GADAVLAASIFHYGRHTVAEAKARLAAGGVVVRPLVTGEPVTGEPVTG"
     gene            complement(5630..6367)
                     /gene="hisA"
                     /locus_tag="VF954_05980"
     CDS             complement(5630..6367)
                     /gene="hisA"
                     /locus_tag="VF954_05980"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX9530666.1"
                     /translation="MLLLPAIDLRDGRCVRLLQGDYARETVYDGDPVAVARDFEASGA
                     PWLHVVDLDAARTGRPANRDVVRAIASSVGVPVQAGGGVRDEFSAEALFDAGVRRIVI
                     GTAALEHPALLARVASRHPGQVAVGLDGRHGEVAVRGWSEGTGVRVLDAVARLADSGA
                     SAVVVTDIGRDGMLAGPDLDGLAAVLAHTQLEVIASGGVAAAADLVALAGLVAGGRRL
                     AGAIVGKALYEGRLSVEEGVQACAASA"
     gene            complement(6368..>6719)
                     /locus_tag="VF954_05985"
     CDS             complement(6368..>6719)
                     /locus_tag="VF954_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX9530667.1"
                     /translation="GATGLGLLPGAVRRLPGDVRLPQMQWNRLDIRRPDSPLLAGLAS
                     RSGDAPWVYFVHSYAPEPGDGVIATCDYGGPVVAAVERDRVWATQFHPEKSGSVGLRI
                     LANFARLCGGAAAA"
BASE COUNT          916 a         2558 c         2429 g          816 t
ORIGIN      
        1 ccggccgtgg tagtcgtcga ccgcgccggg gtcgtcgacg tcgagccggg cgggccgatg
       61 acgggggtcg tggtggagga cgagaccgcg gtcgtcggcg gctcggtggt ggccggcggc
      121 tccgggacgc acggcgggag gttgaggggg aaggtgtcgc tggtgccgac cacggccgag
      181 aggggctgat caccgagctg ctggcagacg accgcgtagg cgaccctccc gttgccggtg
      241 gtggtggccg ggttggcggc gatggtccac gagccggggc ctgtgagcgc gacggacgtt
      301 ccgacgaccg gcacggcccg cacgatcccc tggggatcca ccgaccgggt cgtcacctgg
      361 acgaccagct gggcgggctc gccgacgacc gccgggctgg gcgccagcgc ggccgccacg
      421 ttcacgcccg agaactggtc gaccttcagc gccagctggc gggcctcggc gcctcccagc
      481 cagaacacct gggcatcgag ctcggcgaag tcggggggcc ggtaggcccg cacgcggtag
      541 cgcccgccga ggatgccagg gaggctccag gtcccgtcgg gctgggtcgc cacgtcctgg
      601 gtggcctcgg cgttgcccac gagccgctcg acgtgcacga tcacggcggg cgcggggatg
      661 ggcccgttgg gcccgctgac cgtacccgac aacgaagccg ccccggggcc caccgcgagg
      721 gtggtcgtgg tccggccggg ggccccagcc aggctgacgc ccgacaggtc gggcgcagcc
      781 gtggtggagg tgttcggggg cagcgggggc ggagccttga tcaggctggc ccgtcccgcc
      841 acgcacgagg agagggacag gccgagcgcg gccgcggcca tgatcgccgc gccccgtcgc
      901 ctttctcccg agaaggcgcc gaaagtcatt cttccagcaa aaaaggcgcc agggcgacac
      961 aaaagcatgg aagaatggct ggaggtacgc acccgcaccc agcccgcact caggcggtgg
     1021 ctccggggag ggcagccacg ccggcctcga cccccaggcc gtcccactcg accgaagccg
     1081 cgacagggac ctccacctcg cgccggacat aggccagggc caccacctca ccacctacct
     1141 cgcccggcgc gcaggtccac gagccgcgcg ccgcgctcgt gatctcgccc acgggccggc
     1201 cgtccacgac cacggcggcg ccggccggcg ggaccctctc gccggggatc accacggacc
     1261 gcaggttccg gggcacgtgg ccgccccggg agtcgatccg ggcgaccagc tcctggccgg
     1321 tgaagcaccc tttggtgaag ctcaccgccg ccccgaccag cccggtctcg gccgggatgg
     1381 tgcgctcggt gagctcccgc cccatggccg gcatcccggc ccggacccgc agccattcga
     1441 gctcatccgc cgccacgatc ggcgtgccgg gcggcacggc aggcgccggg ccgagcacgt
     1501 cgacgccctc cacaccaggc cacagggcgg gcacgacgac gccgtcctca ccaggcccac
     1561 cagaggcgac cgcgcccgcg cccggcccgc gcaccgccag gcaccgccag ggcagggcca
     1621 cgatctccgc cttcacccgc agcttgaacc ggcccagcct ctcggcgacc gccggcccga
     1681 acccgccgtc cacgtcgacc aggacggcgt cctccgcccg ccgggtcagg cggacgagcg
     1741 catccacctt cccctgcggt tgcaggacga acgacagctt cgactccccc acgcccaccg
     1801 cggccacgtc ctggctcacc tggccctgga ggaacgacac cgcctcgggc ccggacacga
     1861 gcagcacgtc acgcggccac caggcccata tcgttcccac ccggccatct ttacccgcca
     1921 cgcggctcat cgctcatcgc ggtgcgtggc tggcgcgcgc cgccgacatg cgccgggcgg
     1981 tgggcacggc cgcgaggagg ccggccgcct tgttgcggca ctccaggtcc tcgttggctg
     2041 cgtcgctgtc ggccacgatg cccgcgccgg cctggaccga cgcccggccg tcgggagtga
     2101 cgaccatggt gcggatggcg atggcggtgt ccaggttgcc ggagaagtcg atgtagccca
     2161 ccacgccggc gtagggcccg cgcttggtgg gctccaggtc gtcgatgatc tccatggccc
     2221 ggaccttggg ggcgcccgag accgtgccgg cgggcagggt ggcgcgcagc acgtcgatcg
     2281 gccccagccc ggcgcggagg cgccccgaca cctgcgaggt gaggtgcatg acgtggctgt
     2341 agcgctccag ggtcatcagc tcgtcgacct gctccgtgcc gaagtccacg acccggccca
     2401 cgtcgttgcg ggccaggtcg accagcatga tgtgctcggc gacctccttg ggatgctcgc
     2461 tcagctctcc ggcgagccgc cggtcgtccg cctcggtgcg gccccgccgg cgcgtcccgg
     2521 cgatgggccg tgacacgacc gtgccgtcga gcagctgcac catgggctcc ggcgacgagc
     2581 ccacgatgca gaggcgggga tcccgcacga agtacatgta ggggctgggg ttgacctggc
     2641 gcagggcacg gtagaggtcg aaggggtcgg ccccgaggtc gaggtcgaag cgctgcgaca
     2701 gcacgacctg gaagatgtcc ccggccacga tgtggtcttt ggccacctcg accgcccgcg
     2761 accactccgc cagcgagtgg cctcgcgtca cgtcgggggg tacctcgtcc tgcggcggcg
     2821 gctccacgac cggctcgtcc aggaagcggg cgccgtcggc tgccagggcg tcgacccggg
     2881 ccatcgccgc gtcgtaggcg gcatccaggt ccgccggccg ccaggccggg tcgaccatca
     2941 cgttgtcgat gagctggacc cgctggcgcc agtggtcgaa cgcggccagc tggccgatca
     3001 ccgagaggac cgcgtcgggg tggcccttgt cgtcgcgggg cacgttcggg aggcgctcga
     3061 cctcgcgcac gacgtcgtag cccaggtagc ccaccaggcc accgtgcaac ggcggcaggc
     3121 cggcgatggc cggcgaccgg cagccggcca ggatggcctc cgtggcggcg agcacgcccc
     3181 gatcggtcgg tacgtcgacg ggcaggcgcc cctggacgtc cagggggtgc ccggcgcggg
     3241 ccacgatcgt ggcgaggggg cgccggccga cgaaggagaa gcggctccag cgctcgccgc
     3301 cctcgacgga ctcgaggaga aagccgggct cgtcgccgac cacccggagg aacgccgcga
     3361 ccggcgtgct caggtcggcc aggacctcgc gccatacggg gaccacggag tgcgacgcgg
     3421 cgagagcgtg gaagtcctcc cggctggggt gtcccacggc gtcgcggccc cgggcctagg
     3481 gccggggtgc gcccgggccg gagccgaagg cgcggtagaa gcaggagtgg ttgcccgtgt
     3541 ggcacgcgcc cccaccctcc tggtcgacca ggaagagcag cacgtcgccg tcgcagtcgt
     3601 agtaggcctc gcgcacccat tgccgatcgc ccgacgtgtc gcccttggcc caccgctctg
     3661 cccgggaccg ggaccagtag accatccggc cgctcgacag tgtctcccgc agggtgccgg
     3721 ccgtcatcca ggccatcatc agcacctttc cggtcgaacg ctcctgggcg atggcgggaa
     3781 cgagcccggc ggcgtcgtag gtgatcgagt ccagctcgcc ctcagcgacc tcgatcggcg
     3841 tgaccgcgct cacaggtaca gcccggtgcc accgtcgatg cgctccgacg cgacggcgtg
     3901 gatgtcgcgc tcgcgcagga tgatgtagtc gtcgccctgc acctcgacct cgtagcggtc
     3961 ctccgggttg aacagcacct ggtctcctgg cgtgatgttg cggacgtgag gcccaacggc
     4021 gaccacctcg gcccaggcca accggcgcga cacctgggcg gtcgccggga tcaggatgcc
     4081 cgatcgggac ttgcgctcgc cctcggattg cgggatctgg accaggacgc gatcagccag
     4141 catggtgatg ggctgcttgt gctccttggc gctcaccatc gcaaggtacc gtctggcgcc
     4201 gtggtgatcg agccgatacg cctgcacacc agcgacgagc tggccctgga ggccgagctg
     4261 tccgtgcccc ccgacccccg cgccgcggtg gtcctcgccc acccgcatcc ccgccagggc
     4321 ggcaacatgc gctcgatcgt gcccggtgcg ctgttcgccg ccctgcccgg cctcggcgtg
     4381 gccgccctgc ggttcaactt ccggggcgtg gagggatccg agggcagcta cggcggcggg
     4441 atcggcgagc gccacgacat cgtcgccgcc gtcggggcgc tgcacgacgt gaccgagggg
     4501 ctgccgctcg tgctggccgg ctggtcgttc ggggccgaca ccagcctggc ggtcgtcgac
     4561 gagcggatca cggcctggat ggccatcgcc ccgcccctcc gcaccgcgcc gatcgaggac
     4621 ctggtggcgt cggccgaccc ccgggccaag ctgctcgtcg tcggggacca cgatcagtac
     4681 cgaccgcccg agcaggctcg cccgctggtc gctgcctggg tcaacaccga gctggaggtg
     4741 gtcgccggcg ccgaccactt cttcgtgggc cgcaccgacc ggctggtcga gctggccgtg
     4801 gcctatgtcg accggctcac ccggtgaccg gctcaccggt gaccggctca cccggtgacc
     4861 ggctcaccgg tgaccggctc accggtgacg agggggcgca cgaccacgcc acccgcggcg
     4921 aggcgggcct tggcctcggc caccgtgtgc cgcccgtagt ggaagatcga cgccgccagc
     4981 actgcgtccg ccccgcccgc gacggcgccc tccaccaggt ggtcgagggt gcccaccccg
     5041 ccgctggcga tcaccggaac gttggtggcc tccaccaccg cccgggtgag cgccaggtcg
     5101 aagccgtcgc ccgtgccgtc ccggtccatg gacgtgagca ggatctcgcc cgctcccagc
     5161 cgctcgcaca ccaccgccca gctcaccgcg tcgatgcccg tcggccgccg cccgccgtgg
     5221 gtgaacacct cccaccggcc gcccccgcac gcgcgggcgt cgatggccac caccacgcac
     5281 tgggttccgt actcccgggc cagctcggcg accacggccg ggcgggccac cgccgcggtg
     5341 ttgaccccga ccttctccgc cccggcgcgc aggagccggc ggccgtcctc gaccgagcgc
     5401 acacctccgc ccaccgtgag agggatgaag acctgctcgg cggtgcgccg caccacgtcc
     5461 acgatcgtgg cccgatcgtc ggacgaggcg gtgatgtcca ggaagacgat ctcgtcggcc
     5521 ccctcggcgg cgtagcgcgc cgctagctcg gccgggtcgc cggcgtcgcg gaggttctgg
     5581 aagttgaccc ccttcaccac gcggccgccg tccacgtcga ggcaggggat cacgcggacg
     5641 ctgcgcacgc ctgcacccct tcctcgaccg acagccggcc ctcgtacagc gccttgccga
     5701 cgatggcgcc ggccaggcgc cggccgcccg ccaccaggcc ggccagggcg accaggtcgg
     5761 cggccgccgc caccccaccg gacgcgatca cctcgagctg cgtgtgggcc aggacggcgg
     5821 ccagcccgtc gaggtcgggg ccggcgagca tcccgtcgcg gccgatgtcc gtgacgacga
     5881 cggccgacgc gccgctgtcg gcgagccggg ccacggcgtc caggacgcgc acgccggtcc
     5941 cctcgctcca gccgcgcacg gccacctcgc cgtgccgccc gtcgagcccg acggcgacct
     6001 ggccggggtg acgggacgcc acccgggcca gcagggccgg atgctccagg gcagcggtgc
     6061 cgatcacgat gcgccgcacg ccggcgtcga agagggcctc ggccgagaac tcgtcccgca
     6121 cgcctccacc cgcctgcacg ggcacgccga ccgaggaggc gatggcccgc acgacgtcgc
     6181 ggttggccgg cctcccggtc ctggccgcgt cgaggtcgac gacgtggagc cagggggcgc
     6241 ccgaggcctc gaagtcgcgg gccacggcca ccgggtctcc gtcgtacacc gtctcgcggg
     6301 cgtagtcgcc ctggaggagg cgcacgcacc ggccgtctct caggtcgatg gcggggagga
     6361 ggagcaacta cgccgccgca gcgccgccgc acaggcgggc gaagttggcc agtatccgga
     6421 gcccgaccga tcccgacttc tcggggtgga actgcgtggc ccagacccgg tcccgctcca
     6481 cggccgcgac caccggaccg ccgtagtcgc acgtggcgat caccccgtcg ccgggctcgg
     6541 gggcgtagga gtgcacgaag tagacccacg gcgcatcccc ggagcgcgac gccagcccgg
     6601 cgagcagcgg gctgtcggga cggcggatgt cgaggcggtt ccactgcatc tgcggcagcc
     6661 gcacgtcacc cggcagccgg cggaccgctc ccggcaggag gcccagcccg gtggcgccg
//
LOCUS       DASWGG010000333         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1916366, whole genome shotgun sequence.
ACCESSION   DASWGG010000333 DASWGG010000000
VERSION     DASWGG010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916366"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VF954_05990"
     CDS             complement(<1..343)
                     /locus_tag="VF954_05990"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEX9530668.1"
                     /translation="MPGHLVILSGGLDSTVCMALAAGDGGRPPLALSFDYGQSHRIEL
                     DRAATVARWFGSEHLVVALDATQWGGSALTDPTIAIPDGGVPDGGVPDGGAPDGAATS
                     ADAIPATYVPAR"
     gene            complement(382..606)
                     /locus_tag="VF954_05995"
     CDS             complement(382..606)
                     /locus_tag="VF954_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530669.1"
                     /translation="MANTLMNSRAVVRSIEPGNSALCTSCGAPVKFVARAQLRQVIAN
                     VYVDGAWDRVEHFHADCYGEAGEPYGPPVS"
     gene            complement(836..>2218)
                     /gene="ettA"
                     /locus_tag="VF954_06000"
     CDS             complement(836..>2218)
                     /gene="ettA"
                     /locus_tag="VF954_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX9530670.1"
                     /translation="LLDRYNDVCEAMGDPEADFDKLLAEQSALQDKIDAAGAWDLDRT
                     LDIAMDALRLPPGEVDVTTLSGGERRRVALCRLLLARPDLLLLDEPTNHLDAESVAWL
                     ERHLQEYPGTVVAVTHDRYFLDNVAGWILELDRGRGIPWEGNYTSWLEQKQQRLAMEE
                     KTDQRRQRTLERELEWVRMSPRARQAKGKARLTASEALLAEAASGPERTDRLELPIPA
                     GARLGDLVVEADGLVKGYGDHLLIDGLTFSLPPGGIVGVIGPNGAGKTTLLRMITGQE
                     APDAGALRVGPSVQLAYVDQSRDTLDPGRSVYEEITGGTDRMVVGGREIHGRAYVASF
                     NFKGSDQQKRVGDLSGGERNRAHLAKVLTTGGNVLLLDEPTNDLDVDTLRALEDGLLG
                     FAGCVVVISHDRWFLDRIATHVLAFEGDSRVVWFEGNFSDYESDRHKRLGAEADQPHR
                     IRYKPLVRAP"
BASE COUNT          303 a          750 c          834 g          331 t
ORIGIN      
        1 tccgggcggg gacgtaggtg gccgggatgg cgtcggccga tgtcgcagcg ccgtcagggg
       61 caccgccgtc agggacaccg ccgtccggga caccgccgtc cgggatggcg atggtggggt
      121 cggtcagggc ggagccaccc cactgggtgg cgtcgagcgc gacgaccagg tgctcggacc
      181 cgaaccagcg ggcgacggtc gcggcgcggt ccagctcgat gcggtggctc tgcccgtagt
      241 cgaagctgag ggcgagcggc ggccgcccgc cgtcgcccgc ggccagcgcc atgcacacgg
      301 tcgagtcgag accgccggag aggatgacga ggtggccggg cacggccccc tgaggctagt
      361 ggccccgacg ctgctgcccg gtcaggagac gggcggcccg tagggctcgc cggcttcccc
      421 gtagcagtcg gcgtggaagt gctcgacgcg gtcccacgcg ccgtcgacgt agacgttggc
      481 gatgacctgg cggagctggg cccgggccac gaacttgacc ggcgcaccgc agctggtgca
      541 cagagccgag ttgcccggtt cgatcgagcg cacgaccgcc cggctgttca tcaacgtatt
      601 ggccacgtat gtcttatcgg cacatcttct ccgaagctga gggcggggag ccgggggtgc
      661 atggcgccgg agcacccggc agcctactgt caccccgccg ccacgccttt gcctcaaagg
      721 gcagactgaa cgccgtaatt gacgagtagc gcggaaagat gtgacgcttc caccggcgcc
      781 gggcgtgcgg gtgcgtccgc cgtgcacccg ccccggcgcc gtcattcccc cgtcgtcacg
      841 gggcgcgtac caggggcttg tagcggatgc gatggggctg gtcggcctcg gcaccgagac
      901 gcttgtgccg gtcgctctcg tagtcgctga agttgccctc gaaccagaca acccgggaat
      961 cgccctcgaa ggcgagcacg tgggtggcga tgcggtcgag gaaccaccgg tcgtgggaga
     1021 tgaccacgac gcagccggcg aagccgagaa ggccgtcctc cagggccctc agggtgtcga
     1081 cgtcgaggtc gttggtcggc tcgtcgagga gcagcacgtt gccgcccgtg gtcaggacct
     1141 tggccaggtg ggcccggttc cgctccccgc cggagaggtc gccgacgcgc ttctgctggt
     1201 ccgagccctt gaagttgaag ctggccacgt acgcccggcc gtggatctcc cgcccgccga
     1261 cgaccatccg gtcggtaccg ccggtgatct cctcgtacac cgatcgtccg gggtcgaggg
     1321 tgtcacggga ctggtcgacg taggccagtt gcaccgaggg tcccacccgc aaggcgccgg
     1381 cgtcaggcgc ctcctgcccg gtgatcatgc ggaggagagt cgtcttgccg gcgccgttgg
     1441 gcccgatcac ccccacgatg ccgccgggcg gcaggctgaa ggtcaggccg tcgatcagga
     1501 ggtggtcgcc gtagcccttg accaacccgt cggcctcgac cacgaggtcc ccgaggcggg
     1561 cgccggcggg gatcggcagc tcgagccggt cggtgcgctc cgggccggac gcggcctcgg
     1621 cgagcagggc ctcggaggcg gtgaggcggg ccttgccctt ggcctggcgg gcccggggcg
     1681 acatgcggac ccactccagc tcccgctcca gcgtccgctg ccggcgctgg tcggtcttct
     1741 cctccatggc caggcgctgc tgcttctgct ccagccagga ggtgtagttg ccttcccagg
     1801 ggatgccccg accccggtcg agctccagga tccagccggc cacgttgtcg aggaagtagc
     1861 ggtcgtgggt tacggctacg acggtgccgg gatattcctg gaggtggcgt tctagccagg
     1921 ccaccgactc ggcgtcgagg tggttggtgg gctcgtcgag gaggaggagg tcgggccggg
     1981 cgagcagcag ccggcacagc gccacccggc gtcgctcccc gccggacagc gtggtcacgt
     2041 cgacctcgcc gggcgggagc cgcagggcgt ccatggcgat gtcgagcgtg cggtcgaggt
     2101 cccaggcccc ggcggcgtcg atcttgtcct ggagggcgga ctgttcggcc agcagcttgt
     2161 cgaagtcggc ctcggggtcc cccatggcct cgcacacgtc gttgtaccgg tcgaggag
//
LOCUS       DASWGG010000334         5543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3201758, whole genome shotgun sequence.
ACCESSION   DASWGG010000334 DASWGG010000000
VERSION     DASWGG010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3201758"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VF954_06005"
     CDS             complement(<1..337)
                     /locus_tag="VF954_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX9530671.1"
                     /translation="MAALIPPSDDQGLGAGYHSAQVAVAVRLNTNESPLPPPAAWVAD
                     LQREVGRIDFHRYPDRAASELRAALGELHGVGPEMVFCANGSNEALQTLLLTYGGPGR
                     AAACFEPTYA"
     gene            complement(363..1649)
                     /gene="hisD"
                     /locus_tag="VF954_06010"
     CDS             complement(363..1649)
                     /gene="hisD"
                     /locus_tag="VF954_06010"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX9530672.1"
                     /translation="MLTRLDLRGRPPAELTSDLPRPAAATDPPVAAVQAILAGVRARG
                     DRALLDYTASFDGVTLDSVRVPLAQAEEALAGISPLLREALEAARAAVLAFHREQVGA
                     EGRHERDGIVVRELHRPVQRAGLYVPGGRAIYPSTVLMTAIPARVAGVPELVLCVPPD
                     AGGNVPQATLAAAALAGVDEIYRVGGAQAIGAMAYGTESIRPVDVIVGPGNIYVSVAK
                     REVAGVVGVPSAFAGPSEVVVVADETTPVDFAAIDVIVQAEHGPDGLAWLITWSPEAA
                     DQVTEAVTRLVAESPRRREIEATLSDGGYAVVVDGPEQAMAVANAIAPEHLELMTADP
                     EALVPMVRSAGAVFTGAYAPASVGDYLAGPSHVLPTYGSARFSSALRVDDFRKHVHVI
                     SLDSPALAKVAPHVAAIAEAEGLAAHAESVRLRWQP"
     gene            1741..1929
                     /locus_tag="VF954_06015"
     CDS             1741..1929
                     /locus_tag="VF954_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530673.1"
                     /translation="MTDPLAELSSVTSAVEELTRRVTAMADRFASEKKDDVAADLYAV
                     ERALAGATRRLARVVDGR"
     gene            complement(1863..2630)
                     /gene="nth"
                     /locus_tag="VF954_06020"
     CDS             complement(1863..2630)
                     /gene="nth"
                     /locus_tag="VF954_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX9530674.1"
                     /translation="MVKPRSPKGRAREVTARLAAEYPGTAKDLCALDHETPFQLLVAT
                     ILSAQCTDERVNMVTPAVFARFPTPASLAEADPRQVEELIRPTGFFRNKTRSLIGMAR
                     ATEDRFGGEVPSAIDDLTSLPGVGRKTANVIRGVAFGLPGLPVDTHVGRLSRRLGLTG
                     ATDPVKAELDLNALVPPAQRAAFSLRLILHGRRVCVARRPRCEACLLADFCPSAELPE
                     RAPAKAAAPAGRGGGAQRPSTTRASRRVAPARARSTA"
     gene            2784..3692
                     /gene="mshD"
                     /locus_tag="VF954_06025"
     CDS             2784..3692
                     /gene="mshD"
                     /locus_tag="VF954_06025"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HEX9530675.1"
                     /translation="MRVEVKRQMGRGDIASVSQLLDIAAEVDGHRPLGEHQWLDLVQG
                     GRHGFAGLVAWEPGHDHPVGYAQVSRGSGSWQLEFVVDPHHRAEDLPIGRELVAEAIG
                     VIAGLGGGHVHMWVPRPQAVHDDIAAAAGLARGRDLLQMRRPLPVEGEVPELETRPFR
                     PGEDEGDWLEVNNRAFAWHPEQGDWDLDTLLRREGESWFDPGGFLMHEEGGRLAGFCW
                     TKVHADHDPPLGEIYVVAVDPDRQGTGLGRKLVLVGLDSLARRGLRVAMLYVDAANTP
                     AVALYEDLGFTVDHVDRAYVGDVPGR"
     gene            complement(3754..4533)
                     /locus_tag="VF954_06030"
     CDS             complement(3754..4533)
                     /locus_tag="VF954_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX9530676.1"
                     /translation="MAETQATEATEARARSRAALRLDPADTTLEHHHRNIQGGAARAA
                     VFGVSDGLVTNVSLILGVAGARPAAGFVQLAGLAGLISGAFSMAAGEYISMQAQRELL
                     EHELALERRELARHPEGERRELAHIYVSRGVDRQTAGDLAAEMMRDPDVALETHAREE
                     LGINPSQLGSPLLAGASSFGSFALGAILPLVPWFFLVKGGAAIASIVVGALAAVAIGG
                     ALSVFTGRSWWRSSLRQLAISAGIAALAYGVGAAVGVSGVT"
     gene            4570..>5543
                     /gene="rlmN"
                     /locus_tag="VF954_06035"
     CDS             4570..>5543
                     /gene="rlmN"
                     /locus_tag="VF954_06035"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX9530677.1"
                     /translation="MAAEARSRYELSRAGLAELLGDQPSYRVDQVWRGLYERCCELSE
                     LTDVPRPLRARLSSEPALHRALSPVSTSSSDAGATVKWLWELADGVRVETVLMHYPGR
                     STVCVSTQAGCAMACGFCATGQAGFTRNLRTGEIVEQVALAAREARAAGRRLSNVVFM
                     GMGEPLANYEPVWSAVERLHGELGLSARHLTLSTVGLVPGIDRLAGERLPVNLAVSLH
                     AANDALRSCLVPINRRYPIGALMEACGRYRAERGRRLSFEWALIDAVNDRPSDAAELA
                     GLARPLGAHVNLIPLNPTPGYPTVGSPRHRVVAFRDQLTRLGINATVRR"
BASE COUNT          705 a         2101 c         2035 g          702 t
ORIGIN      
        1 gggcgtaggt cggctcgaag cacgccgcgg ctcgccccgg gccgccgtag gtcaggagca
       61 gggtctggag cgcctcgttg gagccgttgg cgcagaacac catctcgggg ccgacgccgt
      121 gcagctcgcc gagcgcagcc cgcagctcgg acgcggcccg gtccgggtag cggtggaagt
      181 cgatgcgccc cacctcgcgc tggaggtcgg cgacccacgc ggcaggcggc ggcagcggcg
      241 actcgttggt gttgaggcgc accgcgaccg cgacctgcgc ggagtggtag cccgcgccca
      301 ggccctggtc gtccgaaggt gggatcaggg ccgccatcag cccggcggcc gtcgacggag
      361 cgtcacggct gccatcggag ccggaccgac tcggcgtggg cggccaggcc ctcggcctcg
      421 gcgatggccg ccacgtgggg cgccaccttc gccagcgccg ggctgtcgag gctgatgaca
      481 tggacgtgct tgcggaagtc gtcgacccgc agggcgctcg agaagcgagc cgacccgtag
      541 gtgggcagca cgtggctcgg gccggccagg tagtcgccca cgctcgccgg cgcgtaggcg
      601 ccggtgaaga cggcgccggc gctgcgcacc atgggcacca gggcctcggg gtcggccgtc
      661 atgagctcga ggtgctcggg cgcgatggcg ttggcgacgg ccatcgcctg ctccgggccg
      721 tcgaccacca cggcatagcc gccgtccgag agcgtggcct cgatctcccg ccggcgggga
      781 gactcggcga ccaggcgggt gaccgcctcg gtgacctggt cggcggcctc gggagaccag
      841 gtgatgagcc aggccaggcc gtcggggccg tgctcggcct gcacgatcac gtcgatggcg
      901 gcgaagtcca cgggggtggt ctcgtctgcg acgaccacca cctccgaggg gccggcgaag
      961 gccgagggga cgcccaccac cccggccacc tcccgcttgg cgaccgacac gtagatgttg
     1021 ccggggccca cgatcacgtc gaccggccgg atggactcgg tgccgtaggc catggccccg
     1081 atggcctggg ccccaccgac ccggtagatc tcgtccacgc ccgccagcgc ggccgcggcc
     1141 agggtggcct ggggcacgtt ccccccggca tcgggtggga cgcacaggac cagctccggc
     1201 acccccgcga cgcgggcggg gatggccgtc atgagcaccg tgctcgggta gatggcccgg
     1261 ccgcccggca cgtacagccc ggcccgctgg acggggcggt gcagctcgcg cacgacgatc
     1321 ccgtcgcgct cgtggcggcc ctcggcgccg acctgctccc ggtggaaggc gaggaccgcg
     1381 gccctggcgg cctcgagcgc ctcccgcagc aggggagaga tcccggccag cgcctcctcg
     1441 gcctgggcga gcggcacacg cacgctgtcg agcgtcacgc cgtcgaagct ggccgtgtag
     1501 tcgagcaggg cccggtctcc ccgggcccgc acccccgcca ggatggcctg gaccgccgcc
     1561 accggcgggt ccgtggccgc ggccgggcgg ggcaggtcgg aggtgagctc cgccgggggc
     1621 cggccgcgca ggtcgaggcg ggtgagcatg ggcgcccgat cgtacccggc ccgcctctgt
     1681 cccctccggg gattccccgc ggttgaccgc ccgcccgccg ggcgcggcat gatgccggcc
     1741 atgaccgacc cgctcgccga gctgtcgtcc gtcacgtcgg ccgtggagga gctgacgcga
     1801 cgcgtgaccg ccatggccga ccgcttcgcc tcggagaaga aggacgacgt ggccgccgac
     1861 ctctacgcgg tggagcgggc cctcgccggc gcgacccgcc ggctggctcg cgtcgtcgac
     1921 gggcgctgag cgccgccccc gcgccccgcg ggcgccgcag ccttcgccgg ggcccgctcg
     1981 gggagctcgg ccgacgggca gaagtcggcg agcaggcagg cttcgcagcg gggcctgcgc
     2041 gccacgcaga ccctgcggcc atgcaggatg agccgcaggc tgaacgccgc tcgctgcgcc
     2101 ggcgggacca acgcgttgag gtcgagctcg gccttcaccg ggtcggtcgc cccggtcagg
     2161 cccagccggc ggctgagccg gccgacgtgg gtgtccacgg ggagcccggg gaggccgaag
     2221 gccacgccgc ggatcacgtt cgccgtcttg cggcccacgc cggggagcga agtgaggtcg
     2281 tcgatcgcgc tcgggacctc ccctccgaag cggtcctcgg tggcccgggc catgccgatc
     2341 aggctcctgg tcttgttgcg gaagaagccg gtgggccgga tcagctcttc gacctggcgc
     2401 gggtcggcct ctgccagcga cgccggcgtc gggaagcggg cgaagacggc cggtgtcacc
     2461 atgttcaccc gctcatcggt gcactgcgcc gacaggatcg tggccaccag cagctggaac
     2521 ggcgtctcgt ggtccagcgc gcacaggtcc ttggccgtgc cggggtactc cgccgccagc
     2581 cgggcggtga cctcgcgggc gcgacccttc ggcgacctcg gcttgaccat tggggggagg
     2641 ctagggccaa gcgcaaaact acttgggcgg cttctttcag attcgggcgt cacgacttgg
     2701 gacaatcggc ctccggccgc ccaagtagtt ttgcgttcgg ccctaccgac ccgtcatctc
     2761 cgcccaccgg tagcctccgc ggcgtgcgag tcgaggtcaa gcggcagatg ggccggggcg
     2821 acatcgcgtc cgtgagccag ctgctcgaca tcgcggccga ggtcgacggc caccgcccgc
     2881 tcggggagca ccaatggctg gacctcgtcc agggcggccg gcacggcttc gccgggctgg
     2941 tggcgtggga gcccggccat gaccaccccg tgggctatgc ccaggtcagc cggggctccg
     3001 ggagctggca gctcgagttc gtcgtcgacc cccaccaccg ggccgaggac ctgccgatcg
     3061 gccgggagct ggtggccgag gccatcggcg tcatcgccgg cctcggcggc ggccacgtcc
     3121 acatgtgggt gccgcgcccc caggccgtgc acgacgacat cgccgccgcg gccggcctgg
     3181 cccgaggacg cgacctgctc cagatgcgcc ggcccctccc ggtcgagggc gaggtgcccg
     3241 aactcgagac ccgtccgttc cgccccggcg aggacgaggg cgactggctg gaggtcaaca
     3301 accgggcctt cgcctggcac ccggagcagg gagactggga cctcgacacc ttgctccggc
     3361 gggagggcga gagctggttc gaccccggcg gcttcctcat gcacgaggag ggcggccggc
     3421 tggccggctt ctgctggacc aaggtccacg ccgaccacga cccgcctctc ggtgagatct
     3481 acgtcgtcgc cgtcgatccc gatcgtcagg gcacggggct cgggcgcaag ctggtgctcg
     3541 tcgggctcga ctccctggcc cgcaggggcc tccgcgtcgc aatgctgtac gtcgacgccg
     3601 ccaacacgcc cgcggttgcg ctgtacgagg atctgggctt caccgtcgat cacgtcgacc
     3661 gggcctatgt gggcgacgtg cccggccgct gagggacggc tcccgcccgg ggcacccggc
     3721 ctggagccac cccggctcag gtcccgccgg ggctcaggtc acgccggaca cgccgacggc
     3781 tgcgcccacg ccgtaggcga gcgccgcgat cccggccgag atcgccagct ggcgcaggga
     3841 cgaccgccac cacgagcgcc cggtgaacac cgacagcgcc cccccgatcg ccaccgcggc
     3901 cagggcgccc accacgatcg aggcgatcgc cgccccgccc ttcacgagga agaaccacgg
     3961 caccagcggc aggatcgccc ccagcgcgaa ggacccgaac gacgacgccc ccgccagcag
     4021 cggcgagccc agctgggagg ggttgatgcc cagctcctcg cgggcgtggg tctccagggc
     4081 gacgtcgggg tcgcgcatca tctccgcggc caggtccccg gcggtctggc ggtccacgcc
     4141 gcggctcacg tagatgtgcg ccagctcgcg cctctccccc tccgggtggc gggccagctc
     4201 gcgacgctcg agcgccagct cgtgctccag cagctcccgc tgggcctgca tggagatgta
     4261 ctcccccgcc gccatcgaga aggcgccgct gatgagcccg gccaggcccg ccagctgcac
     4321 gaacccggcg gccgggcggg cgccggccac accgaggatc aacgagacgt tggtgaccag
     4381 cccgtcgctg accccgaaca ccgccgcccg ggccgccccg ccctggatgt tgcggtggtg
     4441 gtgctccagc gtcgtgtcgg cgggatcgag ccgcagcgcg gcccgcgacc tggccctcgc
     4501 ctcggtcgcc tcggtcgcct gggtctcggc catacccgcc acgttaccgg cgcccgtacc
     4561 ctgggcggga tggctgccga ggcgcgctcc cgctacgagc tgtcgcgggc cggcctcgcc
     4621 gagctgctcg gcgaccagcc ctcgtaccgc gtcgaccagg tgtggcgcgg cctctacgag
     4681 cggtgctgcg agctgtccga gctgaccgac gtgccccgac cgctgcgcgc cagactgagc
     4741 tcggagcccg cgctgcacag ggcgctgtcc ccggtctcga cctcgagctc cgacgccggc
     4801 gccacggtga agtggctctg ggagctcgcc gacggcgtca gggtcgagac cgtgctgatg
     4861 cactacccgg gccgttcgac ggtgtgcgtc tcgacccagg cgggctgcgc catggcctgt
     4921 gggttctgcg caaccggcca ggcggggttc acccggaacc tccgcaccgg tgagatcgtc
     4981 gagcaggtcg ccctcgccgc ccgcgaggct cgggccgcgg ggcggcggct gtccaacgtg
     5041 gtgttcatgg gcatgggcga gccgctggcc aactacgagc cggtctggag cgcggtcgag
     5101 cgcctccacg gcgagctcgg cctctccgcc cgccacctga ccctgtccac ggtcggcctg
     5161 gtgcccggga tcgaccgcct ggccggcgag cgcctgcccg tgaacctggc cgtgtcgctc
     5221 cacgccgcca acgacgccct gcgcagctgc ctggtgccca tcaaccgccg gtacccgatc
     5281 ggcgccctca tggaggcgtg cggccgctac cgggcggagc gaggccggcg gctctccttc
     5341 gagtgggccc tcatcgacgc cgtgaacgac cggccgtcgg acgccgccga gctggcgggc
     5401 ctggcccggc ccctcggtgc ccacgtcaac ctcatccccc tcaacccgac ccccggttac
     5461 ccgacggtgg gctccccccg ccaccgcgtg gtggccttcc gcgaccagct gacccgtctc
     5521 gggatcaacg ccaccgtccg ccg
//
LOCUS       DASWGG010000335         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3202306, whole genome shotgun sequence.
ACCESSION   DASWGG010000335 DASWGG010000000
VERSION     DASWGG010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3202306"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VF954_06040"
     CDS             complement(<1..749)
                     /locus_tag="VF954_06040"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX9530678.1"
                     /translation="MADARRWWRAAQFPIGVVLAGVALYVVFGKRDEIEGASAYLDHL
                     RLYWVAVAVLAEGVALFAFAGLQRRLLASGRLPVGYGPLLAVTYAGNAIANSLPGGPA
                     WGSVYAYRQFRRLGADETLAAWTLVAVSVCSGAGLALVAAAGLAVAGLDQGADLNLVI
                     VIVGALVVIVGGAALLRRRATVERPAVRLVQLVQRVFHRPAGDPAVVVADLLSRLTRI
                     SPSRGEWTEAMGWATANWLWDSGCLALCFAAV"
     gene            complement(773..>1529)
                     /locus_tag="VF954_06045"
     CDS             complement(773..>1529)
                     /locus_tag="VF954_06045"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEX9530679.1"
                     /translation="GGGGGGSIVGLVILLVIIFVVVALISARRRQQPVEPPFEDHLSG
                     RPAWSPPPGGASVPDTAEEGGGGGLAAIQAHDPAFDVAAFTASVERAFFTVQEAWTEQ
                     KPDMSRQVMADGVWQQHRAQIEGYQRSGRRNVLEGLTVGRADIIRAASDATYDTITVR
                     FLAACADYDIDTASNKVVRGNKSVDQWSEDWVFQRSAAATTKADGGTLQKKCPNCGAP
                     LNLDLAGVCHYCRAPVMSGDYDWVLTRIDQVAV"
BASE COUNT          237 a          560 c          544 g          188 t
ORIGIN      
        1 acggccgcga agcacagggc caggcagccg gagtcccaga gccagttcgc ggtggcccag
       61 cccatggcct cggtccactc gccgcgtgag ggcgagatgc gcgtcaggcg agacagcaga
      121 tcggcgacca cgacggcggg atcgccggcc gggcggtgga acacccgctg gaccagctgg
      181 acgagccgca cggccgggcg ctcgacggtg gcccggcggc gaaggagggc cgccccgcca
      241 acgatcacga ccagcgcgcc cacgatgacg atcacgaggt tgagatcggc tccctggtcg
      301 agccccgcga cggccaaccc ggcggcggcc accaacgcca ggccggcgcc ggagcagacg
      361 ctgacggcga cgagcgtcca cgccgcgagc gtctcgtccg cgcccaggcg ccggaactgg
      421 cggtaggcgt acacgctccc ccacgccgga ccgccgggaa gggagttggc gatggcattc
      481 cccgcgtacg tcacggcgag gagcgggccg tacccgacgg gcaggcggcc cgaggcgagc
      541 agccggcgtt gaaggccggc gaaggcgaac agggcgacgc cctcggcgag cacggccacc
      601 gcgacccagt acaaccgcag atggtcgagg tacgcgctcg ctccctcgat ctcgtcccgt
      661 ttcccgaaca cgacgtacag cgccaccccg gccaggacca cgccgatggg gaactgcgcg
      721 gcccgccacc atcggcgcgc gtcggccatc ctgccgcgag gccgggaagt ctctatacgg
      781 cgacctggtc gatccgggtc aacacccagt cgtagtcgcc gctcatgacc ggcgcccggc
      841 agtagtggca cactcccgcc agatccaggt tgaggggggc gccacagttg gggcacttct
      901 tctggagggt gccgccgtcg gccttggtcg tggccgcggc cgatcgctgg aagacccagt
      961 cctcgctcca ttggtccacg ctcttgttgc cgcgcacgac cttgttggat gccgtgtcga
     1021 tgtcgtagtc ggcgcacgcc gcgaggaagc gcaccgtgat cgtgtcgtag gtggcgtcgc
     1081 tcgcggcccg gatgatgtcg gcccgcccga ccgtcaggcc ctccaggacg ttgcggcgcc
     1141 ccgagcgctg gtagccctcg atctgggccc ggtgctgctg ccagacgccg tcggccatga
     1201 cctggcggct catgtccggc ttctgctcgg tccacgcctc ctgcacggtg aagaacgccc
     1261 tctcgaccga ggccgtgaac gcggcgacgt cgaaggccgg gtcgtgggcc tggatggcgg
     1321 ccagaccgcc ccctccacct tcctcggcgg tgtccgggac ggaagcacca cccggtggcg
     1381 gcgaccacgc cggccggccg gacaggtggt cctcgaaggg cggctccacg ggctgctggc
     1441 ggcggcgagc gctgatcagg gcgaccacca cgaagatgat cacgagcagg atgacgagcc
     1501 ccacgatcga tccgccccca ccgccgccg
//
LOCUS       DASWGG010000336         1894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3202598, whole genome shotgun sequence.
ACCESSION   DASWGG010000336 DASWGG010000000
VERSION     DASWGG010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3202598"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="VF954_06050"
     CDS             <1..1496
                     /locus_tag="VF954_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9530680.1"
                     /translation="AEPPPGEFPFTRGIRKDAYRGRLWTMRQYAGFGTAAESNKRYRY
                     LLERGQTGLSVAFDLPTQIGYDSDHPRAEGEVGGTGVAISTVEDMEILLQGIPLDFVS
                     TSMTINATAPILLLAYELVAERNGVAADRIRGTVQNDILKEYSARGTYIFPPRQSMRL
                     VTDLFAYCQKRIPRWNVISISGYHMREAGCTAVQEVAFTLANAIAYVSAALDAGVPLD
                     ALARRISFFFACHMNFFEEVAKFRAARRMWARIMKERFHAMEEASWLRFHTQTGGATL
                     TAQQPENNIVRTAYEALAAVLGGTQSLHTNSFDEALSLPTEKAAKIALRTQQVLGFET
                     GVGDTVDPLGGSWFVERLTNEIEGQAEELLERIDAMGGAVAAIEAGWVKRQIEESAYR
                     MQQAIEDQSRVVVGVNRFAEPNDQTPIARPDPGAMASHIARLARVKASRRPDAVAAAL
                     DQVRTVAAGSGNLLYPIREALSVSATLGEICDVLREVFGVYQPRESF"
     gene            1496..>1894
                     /locus_tag="VF954_06055"
     CDS             1496..>1894
                     /locus_tag="VF954_06055"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX9530681.1"
                     /translation="MRVAIVGGTGGLGYGLAVRLAAAGQDVVIGSRSADRAQAAAQRV
                     KTLLGPKLQGSIEGGANAEIVKDATVVMVTVPFPGQADTYKAIKSSIGSATTVIDCTV
                     PLASEVGGRPTRMLGVWEGSAAQQARSICGR"
BASE COUNT          293 a          695 c          635 g          271 t
ORIGIN      
        1 cggccgagcc gccgccgggc gagttcccct tcacccgcgg catccgcaag gacgcctatc
       61 ggggccggct ctggaccatg cgccagtacg cgggctttgg caccgccgcg gagtcgaaca
      121 agcgctaccg gtacctgctg gagcggggcc agaccgggct ctcggtggcc ttcgacctgc
      181 ccacccagat cggctacgac tcggaccacc cccgggccga gggcgaggtg gggggcaccg
      241 gcgtcgccat ctcgacggtc gaggacatgg agatcctgct ccagggcatc cccctcgact
      301 tcgtctcgac gtcgatgacc atcaacgcga ccgccccgat cctgctgctc gcctacgagc
      361 tggtcgccga gcgcaacggc gtggcggcag atcgcatccg gggcacggtc cagaacgaca
      421 tcctgaagga gtactccgcc cgcggcacct acatcttccc gccccgccag agcatgcggc
      481 tggtcaccga cctcttcgcc tactgccaga agcgcatccc ccggtggaac gtcatctcca
      541 tctccggcta ccacatgcgg gaggcgggat gcaccgcggt gcaggaggtc gccttcaccc
      601 tggccaacgc catcgcctac gtctcggccg ccctcgacgc cggcgtgccg ctcgacgccc
      661 tcgctcggcg gatctcgttc ttcttcgcct gccacatgaa cttcttcgag gaggtcgcca
      721 agttccgggc ggcccgccgg atgtgggccc ggatcatgaa ggagcgtttc catgccatgg
      781 aggaggcgtc ctggctgcgc ttccacaccc agaccggcgg ggcgacgctg accgcgcagc
      841 agccggagaa caacatcgtc cgcaccgcct acgaggcgct cgccgccgtc ctcggcggca
      901 cccagtcgct gcacacgaac tccttcgacg aggcgctgtc cctgccgacc gagaaggcgg
      961 ccaagatcgc cctccgcacc cagcaggtgc tggggttcga gaccggcgtc ggcgacacgg
     1021 tcgacccact cggcgggtcc tggtttgtcg agcggctcac caatgagata gaaggacagg
     1081 ccgaggagct tctcgagcgt atcgatgcca tgggaggggc ggtggcggcg atcgaagctg
     1141 ggtgggtcaa gcgccagatc gaggagtcgg cgtaccgcat gcagcaggcc atcgaggacc
     1201 agagccgggt ggtcgtcggc gtcaaccgct tcgccgagcc caacgaccag acgcccatcg
     1261 cccggcccga ccccggcgcc atggccagcc acatcgctcg cctcgcccgg gtcaaggcca
     1321 gtcgccgacc cgacgccgtc gccgcggcgc tcgaccaggt ccgcaccgtc gccgccggct
     1381 ccggcaacct cctctatccc atccgggagg ccctatcggt ctccgccacg ctcggcgaga
     1441 tctgtgatgt gctacgggag gtcttcggcg tgtaccagcc cagggagtcg ttctgatgcg
     1501 ggtcgcgatc gtgggtggga cgggagggct gggctacggc ctggccgtcc gcctggccgc
     1561 cgcagggcag gacgtggtca tcgggagccg ttcggccgac cgtgcgcagg ccgcggcgca
     1621 gcgggtgaag acgctcctcg gccccaagct gcagggctcc atcgaagggg gcgccaacgc
     1681 cgagatcgtg aaggacgcca cggtggtcat ggtcaccgtc cccttcccgg gccaggcgga
     1741 cacctacaag gcgatcaagt cgagcatcgg ctccgccacc acggtgatcg actgcacggt
     1801 gcccctcgcc agcgaggtcg ggggccggcc cacccggatg ctcggcgtgt gggaaggctc
     1861 cgccgcccag caggcccgct cgatctgcgg ccgc
//
LOCUS       DASWGG010000337         3675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1593811, whole genome shotgun sequence.
ACCESSION   DASWGG010000337 DASWGG010000000
VERSION     DASWGG010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593811"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VF954_06060"
     CDS             <1..331
                     /locus_tag="VF954_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530682.1"
                     /translation="DRRSKIEIIADEVGPSLRWASAKISRNERKAAGEGGPGGGGGGY
                     SGQGGGPGGGYGGGGQGGGYGGGQGGAGGPGGGQDGGQGGGQGGGYGPGSGQQTGGSY
                     ADDEEPF"
     gene            335..589
                     /gene="rpsR"
                     /locus_tag="VF954_06065"
                     /pseudo
     CDS             335..589
                     /gene="rpsR"
                     /locus_tag="VF954_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626602.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            772..1221
                     /gene="rplI"
                     /locus_tag="VF954_06070"
     CDS             772..1221
                     /gene="rplI"
                     /locus_tag="VF954_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX9530683.1"
                     /translation="MRIVLRADVDDVGKKGDILEVSDGYARNFLIPKGHAIRATDGVV
                     AQAASMRRSRDIKDARDRSSAETVARTLVPAVIRISARSGTEGRLFGSVTAADVVEAV
                     RAQTGVELDRRRLHMAEPIKTLGSHEVPVKLHSEVEFRVTVEVTAGS"
     gene            1450..2817
                     /gene="dnaB"
                     /locus_tag="VF954_06075"
     CDS             1450..2817
                     /gene="dnaB"
                     /locus_tag="VF954_06075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX9530684.1"
                     /translation="MVQAIDEVRRRARSAGGVGRVPPHNLQAEESLLGAMLLSRDAIA
                     SAVEVVSADDFYKPAHGHVFDAVTSLYGQGEPADPVTVADELRRADLLDAIGGPATLI
                     SLQSNTPATSNAGRYARIVEEHALLRRLIGVANEIAELGYGVPEDVTSAVDRAEAMVY
                     EVAQRRVTDTLSPIRELLHASLDRLEALFERGETITGVATGYTDLDERLAGLQPSNLV
                     IVGARPGAGKTSFALGMVAHAAMEGNVPTLMFSLEMSHLELTQRLLCAEARVDATRMR
                     NGRLLDSDWPKISHAIGRLGEAPIFIDDNPNITVMDIRAKARRLKSREGLGLVVVDYL
                     QLMSGRSTAENRQVEVSEISRGLKILARELNLPVVALSQLSRNLESRSDKRPVLADLR
                     ESGSLEQDADVVMFIYRDELYNPDSADRGTAELIVAKHRNGPTGVAQLAFLDHYTRFA
                     NMARV"
     gene            2913..3533
                     /locus_tag="VF954_06080"
     CDS             2913..3533
                     /locus_tag="VF954_06080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX9530685.1"
                     /translation="MPVDLATSPDAPAGPASTGRPAPGGAATAALAGGCALLVLRPAL
                     WRWGDPTVALTVLFVALLAGGAGWPLPRDRRGEPRAGAAGGRAQVAALLLGVAAFGAG
                     RLLGGGHPPAPVSLRFVGLNTLAAVAEEAFFRRLAYGLLAPAGAGLAVVGTSVLFALA
                     HVGVYGWWVAPLDLAAGLVLGWQRWATGSWAVPALTHVVANVLVVV"
BASE COUNT          491 a         1363 c         1357 g          464 t
ORIGIN      
        1 tgaccggcgg tccaagatcg agatcatcgc cgacgaggtc gggcccagcc tgcgctgggc
       61 gtcggccaag atctcccgca acgagcgcaa ggccgccggc gagggcggcc cgggcggcgg
      121 cggaggcggc tacagcggcc agggcggcgg tccgggtggc ggctacggcg gggggggcca
      181 gggtggcggc tacggcgggg gccagggcgg cgccggcggg ccgggcggag gccaggacgg
      241 cggccagggc ggtggccagg gcggtgggta cggcccgggg tccggacaac agaccggcgg
      301 gtcctatgcc gacgacgagg agccctttta aaccatggcg cggagcaatg accgggaccg
      361 gaagcggacc ccgaaggaca ccggccggcg cggcggcaag cccaaggtct gcatcttctg
      421 caaggaccac atcgaatggg tcgactacaa ggacgtcaac ctgctgcgcc ggttcgtgag
      481 cgaccggggc aagatccggg cccggcgggt caccggcaac tgcgcccagc accagcggga
      541 cgtggccgtc gccgtcaaga cggcgcggga gctggccctg cttccctaca cccagcggac
      601 gatgacggag cggggtggcc gccgcgaggg cggccgccgc ggcgagcccc gctccgggcc
      661 ggcggaggcc gcagggcgcg acagtgctgc acccgacgcg gttgcgcccg ccgtcgaggt
      721 ggtccccgag gggggcgagg ccggcgccga gctcgagacg gcggacgaga catgaggatc
      781 gttctgcgcg ccgacgtcga cgacgtcggc aagaagggcg acatcctcga ggtctccgac
      841 ggctacgccc gcaacttcct catccccaag ggccacgcca tcagggccac cgacggggtg
      901 gtggcccagg ccgcgtccat gcgccggtcg cgagacatca aggacgctcg cgaccggtcc
      961 tcggccgaga cggtggcgcg cacgctcgtt cccgcggtga tccggatctc ggcgcgctcg
     1021 gggacggagg gcagattgtt cggctcggtc accgccgccg acgtggtgga ggccgtgcgg
     1081 gcccagaccg gggtcgagct ggaccggcgg cgcctccaca tggccgagcc catcaagacc
     1141 ctcggcagcc atgaggtgcc cgtcaagctg cactccgagg tcgagttccg ggtcacggtc
     1201 gaggtcaccg ccgggtcctg agcccgccgt gcagcacctg gcgcgggccg cccgcgccgc
     1261 ccgcaacgcc tgagccgggc cgtccgcggc ggcgtcacag accgggcgca tactggggaa
     1321 catgcgtttg gcatggaggg ctgtgggaaa gcccgctgtt catcccccgg aaatccccgg
     1381 ttttccggcg acatccccag gccgtcccct ggaaccgcac agggccccta ggccacggta
     1441 ggggtcctca tggtgcaagc gatcgacgag gtccgccgcc gggctcggtc cgccggcggc
     1501 gttgggcggg tcccgccgca caacctccag gccgaggagt cgctgctcgg cgccatgctc
     1561 ctgtcgagag acgccatcgc ctcggcggtg gaggtcgtgt cggccgacga cttctacaag
     1621 ccggcgcacg gtcacgtgtt cgacgccgtc acctcgctgt acggccaggg tgagcccgcc
     1681 gacccggtga cggtcgccga cgagctgcgc cgggccgacc tgctcgacgc catcggcggc
     1741 ccggccaccc tgatctccct ccagtccaac accccggcca cctccaacgc cggccgctac
     1801 gcgcgcatcg tggaggagca cgccctgctc cgccggctga tcggggtggc caacgagatc
     1861 gccgagctgg gctacggcgt cccggaggac gtgacctcgg ccgtcgaccg ggccgaggcc
     1921 atggtctacg aggtcgccca gcgccgggtg accgacaccc tgtcacccat acgggagctg
     1981 ctccacgcca gcctcgaccg actcgaggcg ctcttcgagc ggggcgagac catcacgggc
     2041 gtggccaccg gctacaccga cctcgacgag cgactggccg gcctccaacc ctccaacctg
     2101 gtgatcgtgg gcgcccgccc cggcgccggc aagaccagct tcgccctggg catggtcgcc
     2161 cacgcggcga tggagggcaa cgtcccgaca ttgatgttct cgctcgagat gagccacctc
     2221 gagctgaccc agcggctgct ctgcgcggag gcgcgggtcg acgccacgcg catgcgcaac
     2281 ggccggctcc tggactcgga ctggcccaag atcagccacg ccatcggccg gttgggcgag
     2341 gcgcccatct tcatcgacga caaccccaac atcacggtca tggacatccg ggccaaggcc
     2401 aggcggctca agagccgcga ggggctcggc ctggtcgtgg tcgactacct ccagctcatg
     2461 tcggggcgca gcacggccga gaaccgccag gtcgaggtgt cggagatcag ccgggggctc
     2521 aagatcctgg cgcgcgagct caacctcccc gtcgtcgccc tctcccagct ctcccgcaac
     2581 ctggagagcc gctcggacaa gcggccggtg ctcgccgacc tacgcgagag cggatccctg
     2641 gagcaggacg cagacgtggt gatgttcata taccgggacg agctgtacaa cccggactcg
     2701 gccgatcggg gcacggcgga gctgatcgtg gccaagcacc gcaacggccc gaccggcgtg
     2761 gcccagctcg ccttcctcga ccactacacc cgcttcgcca acatggcgag ggtgtgaccg
     2821 ctcccgccgg acgccattgg ccggcccgag cctcgggctg accgcctcgc tggctctcgc
     2881 ggaccggccc ggctctccta ggctgaccgc ccgtgcccgt cgacctcgcc acgtctcccg
     2941 acgcgccggc ggggccggct tcgacgggcc ggcccgcccc gggcggcgct gccaccgcgg
     3001 cgctggccgg cgggtgcgcc ctgctcgtgc tgcgccccgc gctgtggcgg tggggggatc
     3061 ccaccgtggc gctgacggtg ctcttcgttg ccctgctcgc cgggggggcg ggctggcccc
     3121 tcccccggga ccgacggggg gagccccgag ccggcgccgc cggcgggcgc gcccaggtgg
     3181 ccgcgttgct cctcggcgtg gccgccttcg gcgccggccg cctgctgggc ggcggccatc
     3241 cccccgcgcc ggtgagcctg cgcttcgtcg ggctcaacac cctggcggcg gtggccgaag
     3301 aggccttctt ccgccgcctg gcctacggcc tgctcgcccc ggccggcgcc ggcctggcgg
     3361 tcgtgggcac gagcgtgctc ttcgccctgg cccacgtcgg ggtgtacggc tggtgggtgg
     3421 cgccgctcga cctggccgcc gggctggtcc tgggctggca gcggtgggcg acgggctcgt
     3481 gggccgtgcc cgccctcacc cacgtggtgg ccaacgtcct ggtggtggtc tgagtgcgac
     3541 gggccgcctc ggcgatcgcc gtttgcgccc ttgtcggcgc cgccctcacc gggtgcggcg
     3601 gcggccgggc cgcggcacac ggcgacgtca ccgtcggcct gctgtacccc caggccgggc
     3661 cccagggacc ccagg
//
LOCUS       DASWGG010000338         7423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_947646, whole genome shotgun sequence.
ACCESSION   DASWGG010000338 DASWGG010000000
VERSION     DASWGG010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947646"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VF954_06085"
     CDS             complement(<1..783)
                     /locus_tag="VF954_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX9530686.1"
                     /translation="MAVTVELIDGTFELFRQHFGQPARPAPEGPELGGLHGVVSSLVA
                     MLGQGVTHLGVATDHVIESFRNDLWPGYKTSAGMPPELLSQFEPLEEAIAALGVGLWP
                     MVELEADDALASAAAVADDDAAVGQVRIWTPDKDLGQCVRDGRVVQVDRRRKDAAGAP
                     TVVDAAGVKAKFGVLPESIPDYLALVGDSADGFPGLPRWGAKSAAAVLARYVHLEDIP
                     EHHWQWDLDVRGATALAATLADQREAALLFRHLATLVVDRSLL"
     gene            complement(821..2161)
                     /locus_tag="VF954_06090"
     CDS             complement(821..2161)
                     /locus_tag="VF954_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX9530687.1"
                     /translation="MPVDTYCPSRRSVPRRLRRTATAVALAGGLVALGPSIPASASIS
                     IINADRAGSGLAPLAESSGLDALAASHAVAMANANSLFHTPNLGAAIGSVVPGWTAVA
                     ENVGVGPSLSAVNTAFMGSSTHRANILGPYTVAGLGTFTSFDGRLWVVEEFTAAPGSP
                     APAPAPRAASIVQPAAPASAGTVVAGITAARPAMPPVASILSLGAGYSEIGTDGATFS
                     YAGATFVGSLAGHRLAAPVLSAAATPSGAGAWLAGADGGVFGLGDATYLGGMNGRGLN
                     APVVAIAATPCGTGYWLASSDGGVFAMGTAPFLGSEAGHLAAPVVAMAATPTGHGYWL
                     VGRDGGVFAFGDAPYLGGVAGQALAAPITGMAPTPSGQGYWLSSADGGVFAFGDATFG
                     GGLSGVALAAPVVGIAVAPGGGYRLVGSDGGVFNFGGATFSGSAATLPVAGWPA"
     gene            complement(2329..2781)
                     /gene="mce"
                     /locus_tag="VF954_06095"
     CDS             complement(2329..2781)
                     /gene="mce"
                     /locus_tag="VF954_06095"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX9530688.1"
                     /translation="MTTSEHDHTSHTHTSGVLTEIDHVGLAVHDLDAAVAWYQQMFGA
                     TVAHRERIESDGVEEALLKVADSYIQLLQPYTDASPLAKFLAARGEGIHHVGYRVADC
                     DEVLRAVKGRGARVIDEHPRTGSRGTTVAFMHPKGAFGTLIELVQDNG"
     gene            complement(2778..3938)
                     /locus_tag="VF954_06100"
     CDS             complement(2778..3938)
                     /locus_tag="VF954_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530689.1"
                     /translation="MPRTVRAYCITAESVARERERVGGDARQMDVSRVIQLDKLELRE
                     MGPADVHLRILAASAEHNVDHAALADTVNIAELRGGRIYPGNSAVAEVLDVGEQVSRF
                     APGDIVVTQCNGEPDEYGFPLRIWAYDQPDSVGWYAEEAVVGEWQVIRAPLGCGLDLW
                     QMAALPLRAPTAYHLWRRALGIYRVKVPRERRAVLNVLGFGGGVSELLLQLARSEGHN
                     AYFCSGSPERREALEAFGVVGIDQRRFNRFAARDDVAAFRKVVRDLTWGVDMHVVCDM
                     LRGPVFAAGLAVAARQGVNVSAGWQLSQTVEYNSTLLSVKQVTLDHVHYETVQGCQAA
                     TELYGKVFRPAVHREVYAFEDLPRAFEEMHLNTQTGIPVIRVADQLPAGLQA"
     gene            4053..5237
                     /locus_tag="VF954_06105"
     CDS             4053..5237
                     /locus_tag="VF954_06105"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9530690.1"
                     /translation="MPGSVIVAGARTPIGKLSGALAGFSGAALGGIAIKAALERAGIP
                     PEQVEYVFIGQVLQAGAGQITARQAAVNGGIPMTVPATTVNKVCLSGLNAIYLADQMI
                     SAGEAEVVVAGGQESMTNAPYLLPEARAGYRAGDKTVVDSMMYDGLFCAFDQVAMGAG
                     TERYSRAREISRARQDELAAASHERASAAIKEGRFADEIVAVEVPQRKGDPIVFDTDE
                     GVRPGTTVESLGTLRGAFAKDGTITAGNASQISDGGAAVVVMSRRKASELGIAPLGEV
                     VGYGQVAGPDPSLLTQPARASLRALEKAGLALSDVDLFEFNEAFAAVGAASMDELGLS
                     DAVVNVNGGAIALGHPIGMSGTRLALTILHELRRRGGGTGVAALCGGGGQGDAIVVRT
                     LA"
     gene            5309..6565
                     /locus_tag="VF954_06110"
     CDS             5309..6565
                     /locus_tag="VF954_06110"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX9530691.1"
                     /translation="MPRLTEQMDQSTAWLREQQDVATGGWSGAPGDPHLSALNTAEAV
                     IALMQVEGEIVRDGAVDAGIGYLRSQQAAHGDDAGAWCRTIEVPDGAPVNHPDVVRTG
                     LALVAFTMAGVPVTDNAVANALGWLQRVRNADGGWGCTPHERSRILPTCQVLLGLLAL
                     PAGDPDTDAAIRAGIGFLLERCRRPGGWFSLTEFPDQLSSPHTIYAVLVLQAARRAGI
                     QSDIAPERSAIDWLLAHQDEARRLVIEELDSAPRPHRYTFLYVTDSLLVQVLGSSANA
                     KDRQALLYRAALFSVKDRIEPTHGACYGFRPFTWSTARALAAMSTAAVYEGGFPERPP
                     ETHAAKARGPLTAVLGVMLVLGAILGFTGSFSASEFGFFTLLVLALLLVYGFIGEKTF
                     ADLVPSVLGAKLSAGGAAADQGAAGQ"
     gene            6567..7217
                     /locus_tag="VF954_06115"
     CDS             6567..7217
                     /locus_tag="VF954_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX9530692.1"
                     /translation="MAFGAIIFDAEGVVIDTEGLWDEAQGELLARRGISYDREQVKPL
                     LSGRSAEDGVRIIKDRYALADEVPALVAERRTIALGLFARVDFVSGFVDFFRAADRTY
                     RTALATAMDPPLFAVADAGLGLSAMFHDRVSTVLQVGGRGKPAPDLFVHAAGLVAESA
                     DRCLVIEDAPHGIEAARRAGMACIALTTTFAAALLTGADVVVSSFDEVDGAIRSLG"
     gene            complement(7273..>7423)
                     /locus_tag="VF954_06120"
     CDS             complement(7273..>7423)
                     /locus_tag="VF954_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEX9530693.1"
                     /translation="TAMYLARLLKPLGVKVTRIASGLPVGGDLEYADELTLGRALEGR
                     REVEG"
BASE COUNT         1016 a         2681 c         2713 g         1013 t
ORIGIN      
        1 caggagcgag cggtcgacga cgagggtcgc caggtggcgg aagaggaggg cggcctcacg
       61 ctggtcggcg agggtggcgg ccagggcggt ggcgccccgg acgtcgaggt cccactgcca
      121 atggtgctcc gggatgtcct ccaggtggac gtaccgggcc agcacggccg cagccgactt
      181 ggcgccccac ctgggcagcc cggggaagcc gtcggccgag tccccgacga gggccaggta
      241 gtcggggatc gactccggca gcaccccgaa cttggccttg accccggccg cgtccaccac
      301 ggtcggggcg cctgctgcgt ccttgcggcg gcggtccacc tgcacgaccc ggccgtcccg
      361 cacgcactgg ccgaggtcct tgtcgggggt ccagatgcgg acctggccca cggccgcgtc
      421 gtcgtcggcc accgcggcgg ccgaggccag ggcgtcgtcg gcctccagct cgaccatggg
      481 ccacagcccg acgcccaggg cggcgatggc ctcctcgagg ggctcgaact gggacagcag
      541 ctccgggggc atgccggcgc tggtcttgta gcccggccac aggtcgttgc ggaacgactc
      601 gatgacgtgg tcggtggcca cgccgaggtg ggtgacgccc tggcccagca tggcgaccag
      661 gctcgacacg acgccgtgca ggccgcccag ctccgggccc tcgggcgccg ggcgggccgg
      721 ttgcccgaaa tgctggcgga acagctcgaa ggtgccgtcg atcagctcga ccgtgactgc
      781 catcgccgcc atcctcgcag ttggcgcctg gtggcggcgg ctacgcgggc cagcccgcga
      841 cggggagcgt ggccgcgctg ccggagaagg tggcaccccc gaagttgaag accccgccgt
      901 cggagcccac cagccggtag ccgccgccgg gcgcgacggc gatccccacc accggcgcgg
      961 ccagagcgac gcctgacagt ccaccaccga aggtggcgtc gccgaaggcg aacacgccgc
     1021 cgtcggcgga gctgagccag tacccctggc ccgacggggt gggcgccatg cccgtgatcg
     1081 gcgcagccag ggcctggccg gcgacgccac ccaggtaggg ggcgtcgccg aaggcgaaca
     1141 ccccgccgtc gcgcccgacc agccagtagc cgtgcccggt cggggtggcc gccatggcca
     1201 cgacgggcgc ggcgagatgg ccggcctcac tgcccaggaa gggcgccgtg cccatggcga
     1261 acacgccgcc gtcgctggag gccagccagt agcccgtccc gcacggggtg gcggcgatgg
     1321 ccacgacggg ggcattgagg ccgcggccgt tcatgccgcc caggtaggtg gcgtcgccga
     1381 ggccgaacac tccgccgtcc gcgcccgcca gccacgcgcc ggcgccgctc ggggtggccg
     1441 cggccgacag cacgggcgca gccaggcggt ggccggcgag gcttcccacg aaggtcgccc
     1501 cggcgtagga gaaggtggcg ccgtcggtgc cgatctcgga gtagcccgcg cccaagctca
     1561 ggatggaggc caccggaggc atggcggggc gggccgccgt gatcccggcc accaccgtcc
     1621 cggcgctggc gggggccgcc ggctggacga tggaggcggc gcggggcgcc ggggccggcg
     1681 ccgggctgcc gggtgccgcc gtgaactcct cgaccaccca cagccggccg tcgaagctgg
     1741 tgaaggtccc cagcccggcg acggtgtagg gaccgaggat gttggcccgg tgcgtcgagg
     1801 agcccatgaa ggccgtgttc accgcgctca gcgaggggcc gactccgacg ttctcggcga
     1861 ccgccgtcca ccccgggacc accgaaccga tggctgctcc caggttgggg gtgtggaaca
     1921 gggagttggc gttggccatg gccacggcgt gggacgcggc cagcgcgtcc aggccgctgc
     1981 tctctgccag cggggccagc ccgctaccgg cccggtcggc gttgatgatc gagatcgacg
     2041 cgctggccgg gatggacggg ccgagcgcca ccaacccgcc ggcgagcgcg accgcggtcg
     2101 cggtccggcg cagccggcgg ggcacagaac ggcgggatgg gcagtaggta tcgaccggca
     2161 tgccgaggcg agctcctctc gcggggagtt gggggggagc accggcacgg cagatcgggg
     2221 ggagcgggct ggcaccggtg gggctcgttc ggcggacaac ctagcccaag gggtccaggg
     2281 gctcgccagt cctaatctcc caattgtccc aatttgccgc gacagcggct agccgttgtc
     2341 ctgcaccagc tcgatgagcg tgccgaaggc ccccttgggg tgcatgaagg ccaccgtggt
     2401 gccccggctg ccggtgcggg gatgctcgtc gatgaccctg gccccccggc ccttcacggc
     2461 ccgcagcacc tcgtcgcagt cggccacccg gtagcccacg tggtggatgc cctctcccct
     2521 ggcggccagg aacttggcca gcggggaggc gtcggtgtac ggctggagga gctggatgta
     2581 ggagtcggcg accttcagca gggcctcctc cacgccgtcg ctctcgatgc gctcgcggtg
     2641 ggccacggtc gcgccgaaca tctgctggta ccacgccacc gccgcatcca ggtcgtggac
     2701 ggccaggccc acgtggtcga tctcggtcag cacaccgctg gtatgggtgt ggctggtgtg
     2761 gtcgtgctcg ctcgtggtca tgcctgcagc cccgcaggga gctggtcggc gacccggatg
     2821 acggggatgc ccgtctgggt gttgaggtgc atctcctcga acgcccgggg caggtcctcg
     2881 aaggcgtaca cctcgcggtg cacggccggc ctgaacacct tcccgtacag ctcggtcgcg
     2941 gcctggcagc cctgcaccgt ctcgtagtgc acatggtcca gcgtgacctg cttgaccgag
     3001 agcagggtgg agttgtactc gaccgtctgg ctcagctgcc atcccgcgct cacgttgacg
     3061 ccctgccggg ccgcgaccgc gaggccggcg gcgaacaccg ggccgcggag catgtcgcac
     3121 acgacgtgca tgtccacgcc ccaggtcagg tcgcgcacga ctttgcgaaa ggcggccacg
     3181 tcgtccctgg cggcgaagcg gttgaaccgg cgctggtcga ttcccaccac gccgaacgcc
     3241 tcgagagcct cccggcgctc ggggctgccc gagcagaagt aggcgttgtg gccctcggac
     3301 cgggccagct ggaggagcag ctcggagacc ccgccgccga agcccagcac gttgaggacg
     3361 gcccgtcgct cgcggggcac cttcacccgg tagatcccca gagccctgcg ccacaggtgg
     3421 tacgccgtgg gcgcccgaag cggcagggcg gccatctgcc acaggtccag cccgcagccg
     3481 aggggggcgc ggatcacctg ccactccccg acgaccgcct cctcggcgta ccagcccacc
     3541 gaatcgggct ggtcgtaggc ccagatgcgc agcgggaacc cgtactcgtc gggctccccg
     3601 ttgcactggg tgaccacgat gtcaccgggg gcgaagcgcg acacctgctc gcccacgtcc
     3661 agcacctcgg cgacggccga gttgccgggg tagatccggc cgccccgcag ctcggcgatg
     3721 ttgacggtgt cggccagggc ggcgtggtcg acgttgtgct ccgccgacgc cgccaggatg
     3781 cggaggtgca cgtcggccgg ccccatctcg cgcagctcga gcttgtcgag ctggatgacc
     3841 cgggagacgt ccatctggcg ggcgtcgccc ccgaccctct cccgctcgcg ggcgaccgac
     3901 tcggcggtga tgcagtaggc gcggaccgtc cggggcatcg gctgtgaccc tagcggcgag
     3961 acccgtgtgt cggcgcgtgt gtccgtaacg gcgggcgccc caccctaaac tggcggggca
     4021 ctcgccccac cctggctcgg gaggtcgccg tcatgcccgg atccgtcatc gtcgcaggag
     4081 cccgcacgcc catcggcaag ctgtctggcg cgctggccgg cttctcgggc gcggccctcg
     4141 ggggcatcgc catcaaggcc gctctcgagc gggcgggcat cccgcccgag caggtcgagt
     4201 acgtgttcat cggccaggtg ctgcaagcgg gcgccggcca gatcacggcc cggcaggccg
     4261 cggtcaacgg cggcatcccg atgacggtgc ccgccaccac cgtgaacaag gtgtgcctct
     4321 ccggcctgaa cgcgatctac ctggccgacc agatgatctc ggccggcgag gccgaggtgg
     4381 tggtggcggg cggccaggag tcgatgacca acgcccccta cctcctgccc gaagcccgtg
     4441 ccggctaccg ggcgggcgac aagacggtcg tggactcgat gatgtacgac gggctcttct
     4501 gcgcgttcga ccaggtggcc atgggagcgg gcaccgagcg gtacagccgg gccagggaga
     4561 tctcccgggc gcgccaggac gagctggcgg cggcatccca cgagcgggcg tcggccgcca
     4621 tcaaggaggg ccgcttcgcc gacgagatcg tcgccgtcga ggtgccccag cgcaagggcg
     4681 acccgatcgt gttcgacacc gacgagggcg tccggcccgg aaccacggtc gagtcgctcg
     4741 gcaccctgcg gggcgcgttc gcgaaggacg gcaccatcac cgccggcaac gccagccaga
     4801 tctccgacgg cggcgcggcc gtcgtggtga tgtcccggcg caaggcctcc gagctcggca
     4861 tcgcccccct cggcgaggtc gtcggctacg gccaggtggc aggtccggac ccgtcgctgc
     4921 tgacccaacc ggcgcgtgct tcgctccgcg ccctggagaa ggcggggctg gccctgtcgg
     4981 acgtggatct cttcgagttc aacgaggcct tcgccgcggt gggcgcggcg tccatggacg
     5041 agctcggcct gagcgacgcc gtcgtcaacg tgaacggtgg cgccatcgcg ctcggccacc
     5101 cgatcgggat gtccggcacg cggctcgccc tgaccatcct gcacgagctg aggcgccggg
     5161 ggggcggcac cggggtcgcc gccctgtgcg gcggcggcgg gcagggcgac gccatcgtgg
     5221 tgcgcaccct ggcgtagccc aggcggctgc tcgtacgctt cgacaccgct cctatccttc
     5281 gacaccgcga tcctgcggag gaggcacgat gccgcggctc accgagcaga tggaccagtc
     5341 gacagcgtgg ctgcgcgagc agcaagacgt ggccaccggt ggatggagcg gggcaccggg
     5401 cgacccacat ctcagcgccc tcaacacggc cgaagcggtg atcgcgctga tgcaggtcga
     5461 gggtgagatc gtcagggacg gcgcggtcga cgccgggatc gggtacctcc gaagccagca
     5521 ggcggcccat ggcgacgacg ccggcgcctg gtgccggacg atcgaggtgc cggacggcgc
     5581 gcccgtcaac catcccgacg tggtccggac cgggctcgct ctcgtggcgt tcacgatggc
     5641 gggcgtgccc gtgaccgaca acgcggtcgc caacgcgctc gggtggctcc agcgggtccg
     5701 caacgccgac ggcgggtggg gctgcacgcc ccacgagcgc agccggatcc tgccgacctg
     5761 ccaggtgctt ctcggccttc tcgccctgcc cgccggggac ccggacaccg acgccgccat
     5821 cagagccggg atcgggttcc tgctcgaacg ctgccgcaga ccgggcgggt ggttctcgct
     5881 caccgagttc cccgaccagc tctcgtcgcc tcacacgatc tacgccgtcc tcgtgctgca
     5941 agccgcccgg cgcgccggca tccagagcga catcgccccc gaacggtccg ctatcgactg
     6001 gcttctcgcc caccaggacg aggcccgtcg tctcgtgatc gaagagctcg acagcgcgcc
     6061 ccggccgcac cggtacacgt tcctctacgt caccgactcc ctcctggtcc aggtgttggg
     6121 gagctcggcc aacgccaagg atcgccaggc cctgctctac cgggccgccc ttttctccgt
     6181 gaaggacagg atcgagccga cccacggtgc ctgctacggg ttccggccct tcacctggtc
     6241 gaccgcccgg gcgctggcag ccatgagcac cgcagccgtc tacgaaggcg gcttccccga
     6301 gcggcccccc gagacgcacg cggccaaggc ccgggggccg ctgacggccg tcctcggtgt
     6361 catgctggtc ctcggcgcca tcctggggtt caccgggagc ttctcggcct ccgagttcgg
     6421 cttcttcacg ttgctcgtcc tggccctgct gctcgtgtac gggttcatcg gcgagaagac
     6481 gttcgccgac ctggtgccct cggtcctggg cgccaagctc tccgcggggg gcgcggccgc
     6541 agaccagggc gcggcgggtc agtagcttgg ccttcggggc gatcatcttc gacgccgagg
     6601 gcgtggtgat cgacacggag gggctctggg acgaggccca gggcgagctg ctcgcccggc
     6661 gcggcatctc ctatgaccgc gagcaggtca agccgctgct ctcaggtcgg tcggcggagg
     6721 atggtgtgcg gatcatcaag gaccggtacg cgctggccga tgaggtcccg gccctcgtgg
     6781 ccgagcggcg gacgatcgcc ctgggcctct tcgccagagt cgacttcgtg agcgggttcg
     6841 tcgatttctt ccgggcggcg gaccggacct accggaccgc gctcgcgacc gcgatggacc
     6901 cgccgctgtt cgcggtcgcg gacgcgggcc tcgggctgtc cgccatgttc cacgaccgtg
     6961 tctccaccgt ccttcaagtc ggcgggaggg gcaagccggc cccggacctg ttcgtccacg
     7021 ccgccgggct ggtcgccgag agcgccgacc gttgcctcgt catcgaggac gcgccgcacg
     7081 gcatcgaggc ggccaggcgc gccggcatgg cgtgcatcgc cctcaccacc accttcgcgg
     7141 cggcgctgct cacgggcgcc gacgtcgtgg tctcctcgtt cgacgaggtg gacggggcga
     7201 tcaggtctct cgggtgaccg acctcgctgc catctgagcc accgactcag ccgtgggctt
     7261 cggccgtcga cttcaccctt cgacctcgcg ccggccttcg agcgctcggc cgagggtcag
     7321 ctcgtcggcg tactccaggt cgccgcccac gggcagcccg ctggcgatcc gcgtgacctt
     7381 gacgccaagc ggcttgagca ggcgggccag gtacatcgcc gtg
//
LOCUS       DASWGG010000339         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2873784, whole genome shotgun sequence.
ACCESSION   DASWGG010000339 DASWGG010000000
VERSION     DASWGG010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2873784"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VF954_06125"
     CDS             complement(<1..880)
                     /locus_tag="VF954_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX9530694.1"
                     /translation="MDLDRFLSVNRPVWDRLGLLTNRAGRGPGRLSAAELDEMVHLYQ
                     RAATHLSYVRTAYQDPALTARLTGLVAAAGAVVYGTRPRTLRAVGRFFTITFPAAVWH
                     IRRPVLVSGLLTLAPAVALGLWLAHSPAAVSAVGPAAARQQYISKDFAQYYSSSPSVE
                     FATRVFVNNVQVAILAFAAGILGCLLTAYVLATNGANLGVAAGLFAAAGRSGQFYGLV
                     APHGLLELSSVVIAGGAGLSLGWALIDPGDRPRARALGEDGRRAMAVVVGLVVCFGVA
                     GTIEGFVTGSGLPTWAR"
     gene            922..1830
                     /locus_tag="VF954_06130"
     CDS             922..1830
                     /locus_tag="VF954_06130"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX9530695.1"
                     /translation="MTTLAGIVTPEAVVLDFDTAGLGSRLVAEVIDLLIQFVAGILVV
                     ITTVLAAFVAPTAAAVVRYVALFLIVFGYPAATETLWRGRTVGKAALGLRVVTVEGAP
                     VRFRHAAIRSALALVDVYLLSGLPGILSILLTARNQRLGDLAAGTIVLRQRSAVGMPV
                     ATVFSVPPPLEAYVASLDVAALTGPEYAAVRSFLLRAAQLAPADRAALAAQLAAPLAG
                     RLGHTPPGTIAPEWYLACVGVAAQRRGAGGPAPPAGGLPHLLGGAPRPSAWDPAELGR
                     ARPAPTAPPAPPAEPVEPVGGFTPPS"
     gene            complement(1923..>2295)
                     /locus_tag="VF954_06135"
     CDS             complement(1923..>2295)
                     /locus_tag="VF954_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530696.1"
                     /translation="GYWFVAADGGIFSFGDAAFYGSTGGIHLNQPIVGMAATPTGRGY
                     WLVGRDGGVFAFGDAAFYGSTGGIHLNQPIVGVTRSVTGHGYRFVAADGGVFSFGDVP
                     FYGSAGGVPLDAPVVATTAGD"
BASE COUNT          302 a          881 c          828 g          284 t
ORIGIN      
        1 cccgggccca ggtgggcagc cccgagccgg tcacgaaccc ctcgatagtc ccggccacgc
       61 cgaagcagac gacgaggccg accacgaccg ccatggcgcg ccggccgtcc tcgccgagcg
      121 cccgggcccg ggggcgatcg cctgggtcga taagcgccca accgaggctc aggccggcgc
      181 cgcccgcgat gaccacgctc gacagctcca gcaggccgtg cggtgccacc agcccgtaga
      241 actgccccga gcgcccggcc gccgcgaaca gcccggccgc cacgccgagg ttggccccgt
      301 tggtggccag gacgtacgcg gtgagcaggc acccgaggat cccggcggcg aaggcgagga
      361 tggcgacctg cacgttgttg acaaagaccc tggtcgcgaa ctcgaccgac gggctggagg
      421 agtagtactg ggcgaagtcc ttcgagatgt actgctggcg cgccgccgcc gggcccacgg
      481 cgctgaccgc ggccggggag tgggccagcc acagcccgag ggcgaccgcc ggcgccaagg
      541 tgaggaggcc gcttaccagc accgggcggc ggatgtgcca gacggcggcg gggaaggtga
      601 tggtgaagaa ccggcccacg gcccgcagcg tccggggccg ggtaccgtag acgacggcgc
      661 ccgcggccgc gaccaggccg gtcagccggg ccgtgagggc aggatcttgg taggcggtcc
      721 gcacgtagga cagatgggtg gcggcgcgct ggtagaggtg gaccatctcg tccagctccg
      781 ccgcgctgag ccgccccggc ccccggccgg cccggttggt cagcaggccc aggcggtccc
      841 acacgggtcg gttgaccgag aggaagcggt cgaggtccac ggccgtccaa cggtaccgcc
      901 cctgctccgg ggctggcctc gatgacgacg ctcgccggca tcgtcacccc cgaggcggtg
      961 gtcctcgact tcgacaccgc cgggctcggg tcccgtctgg tcgccgaggt catcgacctg
     1021 ctcatccagt tcgtggccgg catcctggtc gtcatcacga ccgtcctggc cgccttcgtc
     1081 gctccgactg cggccgcggt ggtccgctac gtcgcgctgt tcctgatcgt cttcggctac
     1141 ccggccgcca ccgagacgct gtggcggggg cggaccgtcg gcaaggccgc cctcggcctg
     1201 cgggtcgtga ccgtcgaggg ggcgccggtg cggttccggc acgcggccat ccgctcggcc
     1261 ctggcgctcg tcgacgtcta cctcctcagc gggttgcccg gcatcctgag catcctgttg
     1321 acggcccgca accaacgcct cggcgacctg gccgccggca ccatcgtgct gcgccagcgc
     1381 agcgccgtcg gcatgccggt ggcgacggtg ttctccgtgc cgcccccgct cgaggcctac
     1441 gtcgcctcgc tggacgtggc cgcactcacg gggccggagt acgcggccgt gcgctcgttc
     1501 ctgctgcgcg ccgcccagct cgcccccgcc gaccgggccg ccctagcggc ccagctggcc
     1561 gccccgctag ccggccggct cgggcacacc ccgcccggca ccatcgcccc cgagtggtac
     1621 ctggcctgtg tcggggtcgc cgcccagcgg cggggcgccg gcgggcccgc gccgccggcg
     1681 ggtgggctgc cgcaccttct cggtggcgcg ccccggccct cggcgtggga cccggccgag
     1741 ctggggaggg cccggcccgc cccgacggca ccgcccgcac cgcccgccga gcccgtcgag
     1801 cccgtcgggg ggtttactcc accgagctga gcctctcacg gcgttctcgg cagcattcat
     1861 gcacattttt cgacactttt gctgccgaga acgggaaacc acctgccccg cccgcaggag
     1921 ggtcagtcgc cggcggtcgt ggccacgacg ggagcgtcga ggggaacgcc ccccgccgac
     1981 ccgtagaacg ggacgtcgcc gaaggagaac acgcccccgt cggccgccac gaagcggtag
     2041 ccgtggccgg tgacgctgcg ggtcacgccc acgatgggct ggttgaggtg gatcccgccg
     2101 gtcgagccgt agaaggcggc gtccccgaag gcgaacacgc cgccgtcccg gcccaccagc
     2161 cagtaccccc gcccggtggg tgtcgccgcc atccccacga tgggctggtt gaggtggatc
     2221 ccgccggtcg agccgtagaa ggcggcgtcc ccgaaggaga agatcccccc gtcggccgcc
     2281 acgaaccagt acccc
//
LOCUS       DASWGG010000340         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2080402, whole genome shotgun sequence.
ACCESSION   DASWGG010000340 DASWGG010000000
VERSION     DASWGG010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080402"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VF954_06140"
     CDS             complement(<1..671)
                     /locus_tag="VF954_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530697.1"
                     /translation="MKRNKFLALGAGLALVAGALVLPATLAGASVTVDPVGGGMFQLD
                     GNAQTSVPVGALGDDWDRVCYSSPGGGAGVCPSGSPGVTAPPNVAVDWVAEPNRNSSI
                     FTGGGSKDPQDVAQWAWKDGSGGLPDKDNLQHGFAARYSEAPTASCPANGAPTCEVLY
                     FGSDRFDNSGDALQGTWFFQNAIGLSGTVGNGGSAFVGVHSPGDVLVISDFSIGGSVS
                     SITVY"
     regulatory      complement(704..787)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(1022..1110)
                     /locus_tag="VF954_06145"
     tRNA            complement(1022..1110)
                     /locus_tag="VF954_06145"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1074..1076),aa:Ser,seq:cga)
     gene            complement(1123..>1565)
                     /locus_tag="VF954_06150"
     CDS             complement(1123..>1565)
                     /locus_tag="VF954_06150"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX9530698.1"
                     /translation="MEVALAEAAAAPSHGDVPVGAAVLVGGEVVAHRHNERELAQDPT
                     AHAELLAVRDASAHLGRWRLDDAIVVSTLEPCPMCAGALVAARVKGLVFGAADPKAGA
                     CGSLYNLGADPRLNHELSVTPGVYAEEAAELLARFFGQRREPRS"
BASE COUNT          247 a          573 c          516 g          229 t
ORIGIN      
        1 ttgtagaccg tgatggacga gacgctgccg ccaatgctga agtcgctgat caccaggacg
       61 tcacccggcg agtggacgcc gacgaaggcg ctgccgccgt tgccgacggt gccgctcagc
      121 ccgatcgcgt tctggaagaa ccacgtgccc tgcagggcgt cgccgctgtt gtcgaagcgg
      181 tccgagccga agtacagcac ctcgcaggtc ggggcgccat tggccgggca gctcgcggtg
      241 ggtgcctccg agtaccgggc ggcgaagccg tgctggaggt tgtccttgtc cggcaacccc
      301 ccggagccgt ccttccacgc ccactgggcg acgtcctgtg ggtccttgga gccgcctcct
      361 gtgaagatcg agctgttgcg attgggctca gccacccagt ccacggccac gttcggcggg
      421 gccgtcacgc ccgggctgcc gctcgggcac acgccagctc ctccgcccgg ggacgagtag
      481 cacacccggt cccagtcgtc gccgagcgcg ccgaccggaa ccgacgtctg ggcgttgcca
      541 tcgagctgga acatgccgcc gcccaccggg tcgacggtca ccgacgcgcc cgccagcgtg
      601 gccggcagga ccagggcgcc ggccacgagc gccaggcccg cgccgagcgc caagaattta
      661 ttcctcttca tgcggatacc ccgccccctc tgtctgttta gtgttcggcg cggtccggtt
      721 tcgccactcg ctccccgcag gcaaggcgac cagaaggtgc ctacagatca acgcgtaccg
      781 cgggtgcccc ttagctcccg cggtcagtgt ggcccggatc ctctcatcga tgcaacccag
      841 aatgagaaaa aacgcgcgga aagtggctga cgccgccaag tcctcccgcc ccacgcctac
      901 taacagtgtc cagaccgtca cgtcacgcga caaagaggac caaacggaga gcgcaggacc
      961 aatgcccttg attgaccagg cccttgaatg cctgggcctt ctcccgaaaa tccaggccaa
     1021 ttggcggagg gagtgggatt tgaacccacg gtgggcagga cccacaacgg ttttcgagac
     1081 cgtccgattc ggccgctctc gcatccctcc ttcgccgtga ggttagctcc ggggctcccg
     1141 gcgctggccg aagaaccggg ccagcagctc ggccgcctcc tcggcgtaca cccctggggt
     1201 gacggacagc tcgtggttga gccggggatc cgcgcccagg ttgtacagcg agccgcacgc
     1261 gcccgccttc gggtcggccg ccccgaaaac cagtcccttc acccgggcgg cgaccagcgc
     1321 cccggcgcac atgggacacg gctccagggt gctgaccacg atggcatcgt ccagccgcca
     1381 gcgcccgagg tgggcggagg cgtcgcgaac ggccagcagc tcggcgtgcg ccgtcgggtc
     1441 ctgggccagc tcccgctcgt tgtgccggtg ggccaccacc tccccgccga cgagcacggc
     1501 ggcgcccacc gggacgtcgc cgtggctcgg cgcggcggcg gcctccgcga gcgcgacctc
     1561 catgg
//
LOCUS       DASWGG010000341         3415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2875374, whole genome shotgun sequence.
ACCESSION   DASWGG010000341 DASWGG010000000
VERSION     DASWGG010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2875374"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VF954_06155"
     CDS             complement(<1..575)
                     /locus_tag="VF954_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530699.1"
                     /translation="MKGAVPRRLFAMRTSAGALILAPVVFAVSLGIFGLPGVIAPAKA
                     AGACGANGVLTSPETCTYTTVGEDTFSVPTDVTAVHVTLVGGKGGAGGGTFLGTHGGP
                     GGFGAAVTADIPVSAESLYVEVGGNGSGLTGGSTSGGAGGSATFALGAGGGAASDIRT
                     VADAAGPTSLSSRLVVAAGGGGGGGGGVPYYG"
     gene            complement(873..1163)
                     /locus_tag="VF954_06160"
     CDS             complement(873..1163)
                     /locus_tag="VF954_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HEX9530700.1"
                     /translation="MIAAFSITPVGVDDSVGAAVAEAVRLVRDSGLPNETNAMFTNVE
                     GDWDEVMGLIKTCVAKLAEKAPRVSVVVKIDYRPAVTEALRRKVDFVEQHLR"
     gene            complement(1501..2211)
                     /locus_tag="VF954_06165"
     CDS             complement(1501..2211)
                     /locus_tag="VF954_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9530701.1"
                     /translation="MDSQLYFRQLLAGRDIAVGNQVAAQMVNFVYLIGDRATGEAVAV
                     DPAWDVEGLVEAAGEDGMRITGALVTHHHPDHVGGSMMGHTVEGLADLLARAPATVHV
                     HEDEARWVQRVTGVSSSDLVPHASGDQIMLGDVAIEVLHTPGHTPGSECFLVDGRLVS
                     GDTLFLDGCGRTDLPGGDPEVMYDSLSKLASLPPDVVVYPGHRYSVPSMGTMAAVLET
                     NQAFQPRSKQEWLAIFGG"
     gene            2342..>3415
                     /locus_tag="VF954_06170"
     CDS             2342..>3415
                     /locus_tag="VF954_06170"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX9530702.1"
                     /translation="MLGIEFPILAFSHCRDVVAAVTNAGGLGVLGAVSHTPEHLEIDL
                     AWIEEQVHGRPYGIDLLLPQKYVGADEGGLDRDALRHLLAPEQQAFVDDILRRYQVPE
                     LPDDVRAQIARSSGLSSVSPRGHGPLLDVAFSRRPRLVASALGSPPPTLIERAHGAGV
                     LVAALAGARVHAERHMAAGADLIVAQGTEAGGHTGEIATMVLVPEVVDAVAPTPVLAA
                     GGIASGRQIAAALALGAEGVWCGSVWLTTEEAETQPAVKEKFLAATSRDTIRSRSLTG
                     KPARMLRSAWTDEWERDDAPAPLGLPLQSALVAEAQSRIGRSASKPGSGANKLANYFV
                     GQVVGQMNQVKPARQVVLDMVTEY"
BASE COUNT          528 a         1259 c         1129 g          499 t
ORIGIN      
        1 ccgtaatacg gtacgccccc gccgcccccg ccgcctcctc ccgccgcgac gacaaggcgg
       61 ctgctcagcg acgtaggacc agcggcgtcc gcgaccgtcc tgatatccga cgcagcgcct
      121 ccaccggctc ccagggcgaa cgtggcactc ccacccgcgc cgccgctggt ggaccctccg
      181 gtgagcccgc tcccgttgcc gccgacctcg acgtacagcg attccgccga gacgggaata
      241 tcagctgtga ctgccgcacc gaagcctccc ggtccaccgt gggtcccaag gaacgtcccg
      301 cccccagcgc cgcccttccc cccgacgagc gtgacatgaa ccgcggtgac gtcggtcggg
      361 acggagaacg tgtcttcgcc gacggtcgtg tacgtgcacg tttccggcga cgtgagcacg
      421 ccattcgcgc cgcaagcgcc agccgctttt gccggcgcga tgacccccgg gagcccgaag
      481 attccgagcg ataccgcgaa gacgactggt gcgaggatca aggccccggc acttgtcctc
      541 atcgcgaaca ggcgccgcgg aaccgcccct ttcataccag ccccccctcg gcagcctgcc
      601 cagtccgagc agcaccttta cgtgccagtt tgcacttagt agggagacca gaagcaatgg
      661 cgcaatctga ctaccccggt gcatggttcg tgagctctgc aacgagccag gcagcccagc
      721 tcaccatcgg acaagttgaa gttccgagga gtcgcgacag atctcatggt tatgcacgct
      781 gaggccgagc accaaggatt cggcgttatg gtcgctgtcc tcaacgatcg gacgagcccc
      841 gaagggcgcg acagagcatt cgcggcccgt gctcagcgca ggtgttgctc aacgaagtcg
      901 accttgcgcc ggagcgcctc ggtgacagcg ggccggtagt cgatctttac caccacgctg
      961 acccgcggcg ccttctcggc gagcttggcc acgcaggtct tgatgagtcc catgacttcg
     1021 tcccagtcgc cctcgacgtt ggtgaacatg gcgttggtct cattgggaag gccactgtcc
     1081 cgcaccaacc ggaccgcctc ggccaccgca gcccccaccg aatcgtccac gcccaccggc
     1141 gtgatggaga acgccgctat cacctctgcc gacctccccc gctggctgtc ggccgatctc
     1201 ggagccggcg ggcgtgcttc ttgtgcaccg cctgggggtg acctccagga ccgcggcgat
     1261 ctcggcccag gtccagcccc cgcccgggcc cgatccacgt ggtgctcctc gagccccgca
     1321 aaggggtcca gctcgtcaac ccaggttgat aacccggcgc cgaccaggag aatccgcgca
     1381 cccgtcgtga gcaggacaaa cgccctcgcc aacttcagcg gcagcgccag cggtcgggag
     1441 tcgcgactcc cgctgtctcc aacttgggcg cgccggatca gagcgccggc gcggcgtccc
     1501 tcacccgccg aagatggcca gccactcctg cttggaccgg ggttggaacg cctggttggt
     1561 ctccagcacg gccgccatgg tgcccatgga gggcaccgag taccgatggc ccgggtagac
     1621 caccacgtcc gggggcagcg acgccagctt gctcaggctg tcgtacatga cctcggggtc
     1681 gccgccgggc aggtcggtac ggccgcaccc gtccaggaac aacgtgtctc ccgacaccag
     1741 ccgcccgtcc accaggaagc actcgcttcc gggcgtgtgc ccaggagtgt gcagcacctc
     1801 gatggcgacg tcgcccaaca tgatctggtc accgctggcg tggggaacca ggtcggagct
     1861 cgagacgcct gtgacccgct ggacccatcg ggcctcgtcc tcgtgcacgt ggaccgtggc
     1921 cggggcccgg gccaggaggt cggccagacc ctcgacggtg tggcccatca tggacccgcc
     1981 gacatggtcg ggatgatggt gggtgaccag cgcgccggtg atgcgcatgc cgtcctcgcc
     2041 ggccgcctcc accagcccct cgacgtccca tgccgggtcg acggccacgg cctcaccggt
     2101 ggctcggtca ccgatcaggt acacgaagtt gaccatctgg gcggccacct ggttcccgac
     2161 cgcgatgtcc cggccggcga ggagctggcg gaagtacagc tggctgtcca tgcgcccgat
     2221 cgtaacgaca gcgaagagtg cccggcactc tggcggcgag ggcccccgag ggcgatgatg
     2281 gttccagccg ccgtgggcgg gccacagggg agagccggcc atgcagacca gggtcagtga
     2341 catgttgggc atcgagttcc ccatcctggc gttcagccac tgccgcgacg tcgtcgccgc
     2401 tgtcaccaac gccggaggtc tcggcgtgct cggcgccgtg tcccatacgc ccgagcacct
     2461 cgagatcgac ctggcctgga tcgaggagca ggtccacggc cgcccctacg gcatcgacct
     2521 gctgctgccc cagaagtacg tgggcgccga cgagggcggc ctggaccgtg acgcgctgcg
     2581 ccatctcctg gcccccgagc agcaggcgtt cgtcgacgac atcctgcgcc gctaccaggt
     2641 ccccgagctg cccgacgacg tccgcgccca gatcgcgcgg agctcgggct tgagcagcgt
     2701 gtcgccccgc ggccacgggc cgctgctgga cgtggccttc tcccgccgcc cgaggctcgt
     2761 ggccagtgcc ctgggctcac ctcctcccac gctgatcgag cgggcccacg gggccggcgt
     2821 cctcgtggcc gccctggccg gggcgcgggt gcacgccgag cgccacatgg cggccggcgc
     2881 ggatctcatc gtggcccagg ggaccgaggc gggggggcac acgggagaga tagccacgat
     2941 ggtcctcgta cccgaggtcg tggacgcggt ggcgcccaca ccggtgctcg cggccggtgg
     3001 tatcgcttcg gggcgccaga tcgccgccgc cctcgccctc ggcgccgagg gggtgtggtg
     3061 cgggtcggtg tggctcacga ccgaggaggc cgagacccag ccggcggtca aggagaagtt
     3121 cctggccgcc acgtcgcgcg acacgatccg ctctcgatcg ctgaccggga agccggcccg
     3181 gatgctgcgc agcgcgtgga ccgacgagtg ggaacgggat gacgcccccg cccccctcgg
     3241 cttgccgctg cagtcggccc tggtcgccga ggcccagtcc cgcatcggcc ggtcggcttc
     3301 caagcccggc tccggcgcca acaagctggc caactatttc gtcggccagg tggtcggcca
     3361 gatgaaccag gtgaagccgg cccgccaggt cgtgctcgac atggtcaccg agtac
//
LOCUS       DASWGG010000342         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1279385, whole genome shotgun sequence.
ACCESSION   DASWGG010000342 DASWGG010000000
VERSION     DASWGG010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279385"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VF954_06175"
     CDS             <1..473
                     /locus_tag="VF954_06175"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX9530703.1"
                     /translation="SVLALGLPGFCAFAFLVRAFSSMQDTRTPFFLYVLENGLNVVLA
                     IPLYQAYGVRGLAGALALAYTAAAGAGAAALRWKAGRLEPGLGRAHLRLAAAAAGAVV
                     AMAVVAHIVEGRGTATRLVDAGAGAVAGVTVFIVAAQAMGATELGTLVSRFRRR"
     gene            470..829
                     /locus_tag="VF954_06180"
     CDS             470..829
                     /locus_tag="VF954_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530704.1"
                     /translation="MIGGWVLKLVVAFGLLAIAAFEIGSPVIARVQLDDSVHQAADNS
                     AQTFFQTRSADAAKATAQQVALDKGIVMLDFSVDDQGSTHVRAEKKAHGIFFDRWKQT
                     RRYYHIVETATGHYNNS"
     gene            841..1719
                     /locus_tag="VF954_06185"
     CDS             841..1719
                     /locus_tag="VF954_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX9530705.1"
                     /translation="MPGIRVVTDSACDLPPQLVKEHGIDVVALSVRFGEEELREELSP
                     AEFWARCARSDVLPSTAAPSAGAFQAAFERAADDGCPGVVCINLSSELSATYQAACNA
                     AGALGGTIPARVIDSRSLTIAQGLMCISAAQLADQGKGIDEVAAAVEDLIPRTRIFGA
                     LDTLENLKRGGRIGAASAFMGSLLSIKPIIEVADGKVEPESRQRTRARSLRYLVDKVR
                     SFPEIELLAVMHGEAPDVGEILDMLAEIHPREDIIVGDVGPVIGTHAGPRVIGVAFQV
                     GAPAPADRDPPPAHPG"
     gene            1734..>2177
                     /locus_tag="VF954_06190"
     CDS             1734..>2177
                     /locus_tag="VF954_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9530706.1"
                     /translation="MAGPDAKAELIPQRVLAGRYHLVRLIAVGGMAEVWEGRDAVLAR
                     PVAVKVLMAHLAARPGFNERFRREAVAAARLVHPHIVATFDAGTTAEGGHDELGFIVM
                     ELISGPTLRQLLEGSHRLPPRLAVAIAVQAADALDHAHRSGVVHRD"
BASE COUNT          296 a          835 c          735 g          311 t
ORIGIN      
        1 tgtccgtgct ggcgctgggc ctccccggat tctgcgcctt cgccttcctg gtccgggcgt
       61 tctcgtccat gcaggacacg cgcacgccgt tcttcctcta cgtgctcgag aacgggctca
      121 acgtcgtgct cgccatcccg ctctaccagg cctacggcgt ccgcggcctc gccggggccc
      181 tggcgctggc ctacaccgcg gccgccggcg ccggcgccgc cgccctgcga tggaaggcgg
      241 gccgcttgga gcccggcctc ggccgggccc acctccgcct cgccgcggcc gccgccggcg
      301 ccgtggtcgc catggccgtg gtcgcccaca tcgtcgaggg ccggggcacc gccacccggc
      361 tggtggatgc cggagccggc gcagtggccg gtgttacggt gttcatcgtg gccgcacaag
      421 cgatgggagc gaccgagctg gggacgctcg tgtcccggtt ccgtcgccgg tgatcggcgg
      481 ctgggtcctc aagctggtgg tggccttcgg actcctcgcc atcgccgcgt tcgagatcgg
      541 ctcgcccgtc atcgcccggg tgcagctcga cgacagcgtg caccaggccg cggacaactc
      601 ggcccagacg ttcttccaga cccgcagcgc ggacgcggcc aaggccaccg cccaacaggt
      661 ggccctggac aagggcatcg tcatgctgga cttctccgtc gacgaccagg gatcgaccca
      721 cgtcagggcg gagaagaagg cccacggcat cttcttcgac cgctggaagc agaccaggcg
      781 ctactaccac atcgtcgaga ccgccacggg ccactacaac aacagctgat cgggggaacc
      841 atgcccggca tccgggtcgt cacggacagc gcctgcgacc ttccgcccca actggtgaaa
      901 gagcacggca tcgacgtcgt ggccctcagc gtccgcttcg gcgaggagga gctccgcgag
      961 gagctgtccc ccgccgagtt ctgggccagg tgcgcccggt ccgacgtcct gccgtccacc
     1021 gcagccccat ccgcgggcgc cttccaggcc gccttcgagc gggctgccga cgacggctgc
     1081 cctggcgtgg tctgcatcaa cctgtcctcc gagctgtccg ccacctacca ggccgcctgc
     1141 aacgccgccg gcgcgctcgg cggcaccatc ccggcgcgcg tcatcgactc ccggtcgctc
     1201 accatcgccc aaggcctcat gtgcatctca gccgcccagc tggccgacca gggcaagggc
     1261 atcgacgagg tggccgcggc cgtcgaggac ctgatcccgc gcaccaggat cttcggcgcc
     1321 ctcgacaccc tcgagaacct caagaggggc gggcgcatcg gggctgcctc cgccttcatg
     1381 gggtccctgc tgtcgatcaa gcccatcatc gaggtggccg acggcaaggt cgaaccggag
     1441 tcccgccagc ggacccgcgc caggtcgttg cgctacctgg tcgacaaggt gcggtccttc
     1501 cccgagatcg agctgctcgc cgtcatgcac ggcgaggcgc ccgacgtcgg agagatcctc
     1561 gacatgctcg ccgagatcca cccgcgggag gacatcatcg tgggcgacgt cggcccggtg
     1621 ataggcaccc acgcgggccc gagggtgatc ggcgtcgcct tccaggtcgg cgccccggcg
     1681 ccagccgacc gcgacccgcc accggcccac cctgggtagg gtgcacaagc gtggtggccg
     1741 gtcccgatgc caaggccgaa ctgatccccc agcgcgtcct cgcgggccgt taccacctcg
     1801 tgcgcctgat cgccgtgggc gggatggccg aggtgtggga gggccgcgac gcggtcctcg
     1861 cccggcccgt cgcggtcaag gtgctgatgg cgcatctggc ggcccggccc gggttcaacg
     1921 agcgcttccg gcgggaggcg gtggccgcgg cccggctcgt gcacccccac atcgtggcca
     1981 ccttcgacgc cggcacgacc gccgaaggcg gccacgacga gctgggcttc atcgtcatgg
     2041 agctgatctc cgggccgacg ctgcgccagc tgctggaggg tagccatcgg ctgccgcccc
     2101 ggctcgccgt cgccatcgcc gtgcaagccg ccgacgccct cgaccacgcc caccggtcgg
     2161 gcgtggtcca ccgcgac
//
LOCUS       DASWGG010000343         6436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1920902, whole genome shotgun sequence.
ACCESSION   DASWGG010000343 DASWGG010000000
VERSION     DASWGG010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920902"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VF954_06195"
     CDS             complement(<1..673)
                     /locus_tag="VF954_06195"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX9530707.1"
                     /translation="MAAHLDRLDVWQRRHPPAAFVFAVVKKFGDDQGGDLAALVAYYG
                     FLSLFPLLLVLVSVLGFVLQGHSDLADQLSRTALRQIPVLGQDIKAGRLHKSGVGLVV
                     GLVGALYGGLGVASAAQNAMNRVWGVPRKRWPNMVKSLERSALLAVAGFGILATTALG
                     GGAGAGSHLGLVVRLAALGVSLGCNVGLILFALWVLTVADVSGRDLWPGALVAAAAWQ
                     VLQALG"
     gene            complement(760..1560)
                     /locus_tag="VF954_06200"
     CDS             complement(760..1560)
                     /locus_tag="VF954_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9530708.1"
                     /translation="MTASVPARQHALPTGELAHDDVPGHDDVVELHRRYVADRSPALR
                     AELVERHAGLARALAHRMAGTSREREDIVQVALLGLLHAVDRFEPDRGVTFTTYAWAT
                     IVGEIKKHHRRDQWGMHVSRQLQEAYIRASVAVDDLTQDLGRSPTVSEIARRSGAAEE
                     TVIAALDVGQAYGLASIDAPLDGSGEGRGIEVADDRDAMEELDQRDELRLMLSRLPVR
                     EREVVRLRFVDEMSQAQIAARIGVSQMQVSRLLAQSLARLRALAREPH"
     gene            1702..2127
                     /locus_tag="VF954_06205"
     CDS             1702..2127
                     /locus_tag="VF954_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX9530709.1"
                     /translation="MATTRKATAQWQGSLIEGSGKVSLDSSGLGTYDVTWASRAEEPA
                     GRTSPEELIAAAHSACFSMALSHALAQAGHAPDALETSAEVTFQPGEGITGIRLKVRG
                     TVPGLGADEFAKAADAAKATCPVSKALTGTTITLDAALA"
     gene            complement(2153..2671)
                     /locus_tag="VF954_06210"
     CDS             complement(2153..2671)
                     /locus_tag="VF954_06210"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX9530710.1"
                     /translation="MQYGFPPSGPGSLASPWRRLGARMLDSVVFLPVDGLVIGLVIAA
                     VAPHVGPMFPRSNPNDPNASTPFPGFFWIELAVVAAALFYGVLLAAYEIIATARYGRT
                     LGKAWLHIRPIRIDRSPLGWGRAFGRTALYFAAGMLGWLGLIDHLWCLWDADSQCLHD
                     KPVGTVVINDAD"
     gene            complement(2832..3779)
                     /locus_tag="VF954_06215"
     CDS             complement(2832..3779)
                     /locus_tag="VF954_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX9530711.1"
                     /translation="MALLPRAAGAALVLFIAAGWLAHPAAGRGEGCSPAPVVGCPSAA
                     PPTPVPPPAVLGPSPSPSASPPPSPGPAPRPPAGPLAQPPGPTGTPASFPAAGEVPGA
                     VSHLLQLVNQERAAVGARQLVSRAEILVVAHDHSVEMASRGAIFHNDAYFTAQSHLRL
                     GASVFGENVAMNTSLDDAHRRLMASPGHRANILDARFRQVGFSVVSDGRDELFVTEDF
                     AQPVGASAAPAPAGHDLARDAGTPAELLSATIAAYAGHPPTVLAAVSGPVEVPELVRP
                     ARSARRFPWAPALVAVVLLAMALVGVLSLGSRALLPPRL"
     gene            complement(3936..5132)
                     /locus_tag="VF954_06220"
     CDS             complement(3936..5132)
                     /locus_tag="VF954_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX9530712.1"
                     /translation="MDEEVLSGISLDALSVTIEQLAAAQMAMLVGLLELIGEYDRRQG
                     WAADGMASMTDWVAARLAVSQATSTAWVEAAARLAQLPELRAVAAAGGLSFDQLVPLL
                     VLATPQSDARWAAEGPALKASQLRQLARRARPVTPDDDRSAHERRSLRSYWRRDGMLG
                     LTGRLTGDAGAVVAAALRRLAEGPCGAVEGTERATYEQRCADALVQLASTRMAADTDA
                     DAAAVVVHAPVAVLGGQEGNADVEGGPVISGETLRRLACDCTMRWVLYDHDGAVTVTE
                     AKAALPRWLRRLVKARDLTCRFPGCERRGFGQVHHVIWRSRGGQAEMGCLAYLCWSHH
                     RYVHEGGWDLAGDADGELVFTSPQGRRYTTRPAGLERQAATRLFGPDWDPGSRPPGPP
                     TPPSRN"
     gene            complement(5378..6379)
                     /locus_tag="VF954_06225"
     CDS             complement(5378..6379)
                     /locus_tag="VF954_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX9530713.1"
                     /translation="MRAWIVESPGPIDGGPLRRVDRPAPAPRPGEIRLRVSVCGVCRT
                     DLHLAEGDLPAHGPQIVPGHEVVGTVDALGPGAERFAVGDRAGIAWLRHTCGVCRFCR
                     RGAENLCLDPRFTGWDADGGYADHAVVDERYAYRLPTGYTDEELAPLLCAGIIGYRAL
                     RRSALPAGGRLGVYGFGGSAHLAVQVAMAEGATVYVLTRSPAARQLALDLGAAWAGDT
                     FDPPPEQLDAAIVFAPAGEVVPAALAAIDRGGTVSIAGIHMSAIPTLRYAEHLFFERQ
                     LRSVTANTRQDGEEFLALAGRIGIRVTTTPYPFDGADQALVDLAHDRVSGAAVVRMS"
BASE COUNT         1012 a         2323 c         2302 g          799 t
ORIGIN      
        1 cacccagggc ttggaggacc tgccaggcgg ccgccgcgac cagggcgccg ggccacaggt
       61 cgcgccccga gacgtccgcg accgtaagga cccacagggc gaacaggatc agccccacgt
      121 tgcagcccag ggacacgccg agggccgcca gccggacgac caacccaaga tggctgccgg
      181 cccccgcccc gccgccgagc gcggtggtgg ccaggatgcc gaagccggcc acggccagca
      241 gcgcgctccg ttcgaggctc ttgaccatgt tgggccagcg cttgcggggc accccccaca
      301 cgcggttcat ggcgttctgg gcggcgctgg ccacgccgag acctccgtac aacgcgccca
      361 cgagaccgac tacgagaccg acaccactct tgtgcaatcg accggccttg atgtcctgcc
      421 ccagcacggg gatctgtcgg agggccgttc ggctcaactg atcggcgaga tcggagtggc
      481 cctgcaggac gaaccccagc accgacacca ggaccaggag caacgggaaa agcgacagga
      541 acccgtaata cgcgacgagg gccgccaggt cgcctccctg gtcgtcgccg aacttcttga
      601 ccacggcgaa gacgaacgcc gcgggcgggt ggcgtcgttg ccagacgtcc agccgatcca
      661 ggtgagcggc caccccgacc tcctttcctg gacgccgtgt tgcctcttta ccccgttcgc
      721 tcgcctacca cgcctcgacc agccagtggg gcgggcgcct cagtgaggtt cgcgggccag
      781 ggcgcgcaac cgggccaggc tctgcgccag caggcgtgac acctgcatct ggctcacacc
      841 gatccgggcg gcgatctgcg cctggctcat ctcgtccacg aaccggagtc ggaccacctc
      901 ccgctcgcgt actggcaacc gcgacagcat cagccgcagc tcgtcgcgtt ggtccagctc
      961 ctccatggcg tcgcgatcgt cggcgacctc gatcccccga ccctcgcccg agccatccag
     1021 gggggcgtct atggaggcga gaccgtacgc ctgcccgacg tcgagggcgg cgatgacggt
     1081 ctcttcggcg gccccgctcc ggcgggcgat ctcgctgacg gtgggcgagc ggcccagatc
     1141 ctgggtcagg tcgtcgaccg cgacggaggc ccggatgtag gcctcctgca actgccgcga
     1201 gacgtgcatt ccccactggt cccgtcggtg gtgcttcttg atctcgccca cgatggtggc
     1261 ccatgcgtag gtcgtgaagg tgacgccgcg gtcgggctcg aagcggtcga cggcgtggag
     1321 caatccgagc agggcgacct ggacgatgtc ctcccgttcg cgggacgtcc ccgccatgcg
     1381 gtgggccagc gcccgggcca gcccggcgtg gcgctcgacc agctcggccc gcaacgccgg
     1441 cgaacggtcg gccacatacc gccggtggag ctcgacgacg tcgtcgtggc ccggcacgtc
     1501 gtcgtgggcc agctccccgg tggggagggc gtgctgccgg gccggcacgg acgctgtcat
     1561 gtcccagtag cgactggcga gcccctggcc cacaaagccc tattcccccg acaacccacg
     1621 tcctaccgta ccgcccgatc gggtgagcga caaccgtctt cgtgagacgc cgcctacgac
     1681 cgcgccgcta ggcttcgagc catggcgaca acacggaaag caaccgcaca gtggcaaggc
     1741 tccctcatcg aggggtcggg caaggtgtcg ctcgactcct ccgggctggg cacctacgac
     1801 gtcacctggg cgtcgagggc ggaggagccg gccggccgga ccagccccga ggagctcatc
     1861 gccgcggccc actcggcctg tttctccatg gccctgtcgc acgccctggc ccaggccggc
     1921 cacgcgcccg acgcgctgga gacgtcagcc gaggtcacct tccagcccgg ggagggcatc
     1981 accggcatcc gcctgaaggt ccgcggcacg gtgccggggc tcggggcgga cgagttcgcc
     2041 aaggccgccg acgcggccaa ggccacgtgc ccagtcagca aggcgctgac cggcacgacc
     2101 atcaccctgg acgccgctct cgcctagagg agaggcgtag ggcgctcggc cgtcagtcgg
     2161 cgtcgttgat tacgacggtg ccgacgggct tgtcatggag gcactgggag tcggcgtccc
     2221 agaggcacca gagatggtcg atcagaccga gccagcccag catcccggcc gcgaagtaca
     2281 gcgccgtacg gccgaaggcg cggccccatc ccaggggtga gcgatcgatc cggatgggac
     2341 ggatgtgcaa ccaggccttg ccgagggttc ggccgtaccg ggcggtggcg atgatctcgt
     2401 acgcggcaag gaggacccca tagaagagag ccgcggccac cacggcgagc tcgatccaga
     2461 agaagcccgg gaacggcgtg gaggcattgg ggtcgttcgg gttcgagcgg ggaaacatcg
     2521 gcccgacgtg aggggccacc gccgcgatga ccaggccgat caccagtcca tcgaccggga
     2581 ggaacacgac gctgtcgagc atccgggccc ccaggcggcg ccacgggctg gccagcgagc
     2641 cgggccccga cggcggaaaa ccgtactgca tgcccttcca ggtcggagga ccccagcccc
     2701 atccggccgg cccgtaagcg ggaggcggcg gaggtggcca tccaggtgga ggtggccaac
     2761 cggggggcgg tcctgaaccc catcccccac catccggtgc atcccccatc ggcaccagcg
     2821 tggcacaggc ttcacagcct cggtgggaga agagccctcg acccgagcga gagcaccccg
     2881 acgagcgcca tggcgagcag aaccacggcg accagcgccg gcgcccacgg gaaccgtctg
     2941 gcggaccggg ccgggcgtac cagctccggg acctcgaccg gtcctgagac cgcagccagg
     3001 accgtgggtg ggtgtccggc gtaggcagca atggtcgcgg acagcagctc ggccggcgtg
     3061 cccgcgtccc gggcaaggtc gtgtcccgcg ggcgcaggcg cggcgctggc ccccacgggc
     3121 tgggcgaagt cctcggtcac gaacagctcg tcccgaccat cggataccac cgagaagccg
     3181 acctgcctga acctggcatc gaggatgttg gcccgatgcc cagggctggc catcaggcgc
     3241 ctgtgagcgt cgtcgaggct cgtgttcatg gccacgttct ccccgaagac gctggcgccg
     3301 aggcggaggt ggctctgggc cgtgaagtag gcgtcgttgt gaaagatggc cccacgcgag
     3361 gccatctcga ccgagtgatc atgggccacc accaagatct ccgcccggga gacgagttgg
     3421 cgggcaccca cggcggctcg ctcctgattg acgagctgga gcagatggga gacggcgccg
     3481 ggtacctccc ccgcagccgg gaacgacgcc ggtgtgccag tcggtcccgg gggctgggcg
     3541 agcgggccgg ccggaggccg cggagctgga ccgggcgagg gcggaggcga agccgagggc
     3601 gagggcgagg ggcccagcac cgccggcggc gggaccggtg tcgggggagc cgcagacggg
     3661 cagccgacga cgggcgcggg cgagcagccc tccccgcgcc ctgcggcggg atgggcgagc
     3721 cacccggccg cgatgaagag caccaatgcg gccccagccg ccctcgggag aagagccatg
     3781 atccatgtgt cgcctcaatc gtttgcgaca ttgaggaaat ctttcccatt acccgccaaa
     3841 ccgctcttct cggcctccaa agggcaagag gcgcgtcgca ccgtcaggcg tcggcatcca
     3901 caattctggg ccctcgccgc ctgcccgccg gcccctcaat tccggctcgg cggtgtaggt
     3961 ggcccgggtg gtcggctgcc ggggtcccag tcggggccga acagccgggt ggcggcctga
     4021 cgctccaggc cggccgggcg ggtagtgtag cgccggccct gggggctggt gaagaccagc
     4081 tcgccgtcgg cgtcgccagc aaggtcccag ccgccctcgt gcacgtatcg gtggtgtgac
     4141 caacagagat aggcgaggca gcccatctcg gcctggccgc cccggctgcg ccagatgacg
     4201 tggtgcacct ggccgaagcc ccgtcgctcg cagcccggga accgacaggt caggtcccgg
     4261 gccttgacca gccggcgcag ccagcgcggc agggcggcct tggcctcggt gacggtgacg
     4321 gcgccgtcgt ggtcatacag cacccagcgc atggtgcagt cacaggccag gcggcgcagc
     4381 gtctcgcccg agatgaccgg cccgccctcc acatcggcgt tgccctcctg accgcccagc
     4441 acggccacgg gggcatggac gaccacggcg gcggcatcgg cgtcggtgtc ggcggccatg
     4501 cgggtggagg ccagctgcac cagggcgtcg gcgcagcgct gctcgtatgt ggcccgttcg
     4561 gtgccctcga cggctccaca gggcccctcg gccaggcggc gcagagcggc ggccaccacc
     4621 gccccggcgt cgccggtgag ccgtccagtg aggcccaaca tgccatcgcg gcgccagtag
     4681 ctgcgcaggc tgcgccgctc atgggcgctg cggtcgtcat ctggggtgac gggcctggcc
     4741 cggcgggcca gctggcgcag ctgggaggcc ttcagggccg ggccctcggc ggcccatctg
     4801 gcgtcgctct ggggggtggc caacacgagc aggggcacca gctggtcgaa ggacagcccc
     4861 ccggcggcgg ccaccgcccg cagctcgggc agctgcgcca gccgggcggc ggcttccacc
     4921 caggccgtgg acgtggcctg ggacaccgcc aggcgcgccg ccacccagtc ggtcatcgag
     4981 gccatgccgt cggcggccca tccctgccgg cggtcgtact cgccgatcag ctccaacagc
     5041 cccaccagca tggccatctg ggcggcggcc agctgctcga tggtcaccga cagcgcatcc
     5101 aggctgatgc cggacaagac ctcttcgtcc accgcacaca cactcccgcc aacacgagct
     5161 acatgacaag acccacgtga catgagcggc cagggcggga ggccacgatc ccaggagacg
     5221 actcgcccgg gcccgagaag acccgctgcg aacgtgtgtt cccaccgtag catcctcgcc
     5281 gcgagatgca aggggcaagg ggcaaggggc aaggggcgga aatttctccg gacctgcgcg
     5341 ccgaggagca gatcggttgt tggttctcgg ccaatcctca cgacatccga accacggccg
     5401 ccccgctgac acggtcgtgg gccaggtcga ccagggcctg gtcggcgccg tcgaaggggt
     5461 acggcgtggt ggtcacccgg atgccgatgc ggcccgcgag ggcgaggaac tcctcaccgt
     5521 cctggcgcgt gttggccgtc acgctgcgca gctgccgctc gaagaacagg tgctccgcgt
     5581 accgcaaggt cgggatggcc gacatatgaa tgccggcgat cgacaccgtc ccaccgcggt
     5641 cgatggcggc gagcgcggcc ggcacgacct cccccgccgg ggcgaacacg atcgccgcgt
     5701 cgagctgctc gggcggcggg tcgaacgtgt ctcccgccca cgccgccccc aggtcgaggg
     5761 cgagctgccg ggcggccggc gagcgggtca ggacgtagac cgtggcgccc tcggccatgg
     5821 ccacctggac cgccaggtgc gcggatccgc cgaagccgta gacaccgagc cggcctcccg
     5881 ccgggagggc tgaccggcgc agggcgcggt atccgatgat gcccgcgcag aggaggggtg
     5941 cgagctcctc gtcggtgtag ccggtgggaa gccggtacgc gtaccgctcg tcgacgacgg
     6001 catggtcggc gtagccgccg tcggcatccc agccggtgaa gcgcgggtcg aggcagaggt
     6061 tctcggctcc gcgccggcag aagcggcata ccccgcacgt gtgccggagc cacgcgatcc
     6121 ccgcccgatc cccgaccgcg aagcgctcgg cgcccgggcc cagagcatca accgtcccga
     6181 ccacctcgtg gcccgggacg atctgaggcc cgtgcgcggg caggtctccc tcggccaggt
     6241 gcagatcggt tcggcacacg ccgcacaccg acacccggag ccgtatctca cctggccgcg
     6301 gcgccggcgc gggacggtcg acccggcgga gagggccgcc atcgatcggg ccgggggact
     6361 cgacgatcca ggcgcgcacg ggcctgttct acctcgccgg ggcgctcacg ccgccacagg
     6421 aggaccgggg cctcgc
//
LOCUS       DASWGG010000344         7853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1598362, whole genome shotgun sequence.
ACCESSION   DASWGG010000344 DASWGG010000000
VERSION     DASWGG010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598362"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VF954_06230"
     CDS             <1..240
                     /locus_tag="VF954_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530714.1"
                     /translation="GLLVVFLAVVSVGSMRLLTRADQPPLVHAAGALCLGVLVPLVTA
                     PFLTTTLRWPALVGIAAGVVCEQMDAQGDGASTPV"
     gene            261..1373
                     /locus_tag="VF954_06235"
     CDS             261..1373
                     /locus_tag="VF954_06235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9530715.1"
                     /translation="MDHTAQLSGGQLAMARLIGGLHEQVDVQVVLGEDGPLVHALQVA
                     GADVVVMPIDRSAATLARRDVALRSLPLVPAAKAAAHGWELARHLRRTRPDVVHLNTL
                     KALFYGGVAARLAGRPAVWHARDRITPDYLPAGAVAVTRLAARTLPSFVIANSVATLS
                     TLPLRRPHAVVPSPVDAAAFVTAAPPPGPLAVGMVGRIAPWKGQDVLLRAFALAFPGG
                     DASVRLIGAPLFGEEAYERRLHDLADELGIAGRVAFGGFSDDVPGELGRLHVLVHASV
                     LPEPFGQVVVEGMAAGLPVVATRAGGPAEIVTEGRDGLLVAPGDAAELAGALRRLALD
                     ADLRSRLGAAARETAGRFRVEAVAGQVMGIYAALAR"
     gene            complement(1399..1743)
                     /locus_tag="VF954_06240"
     CDS             complement(1399..1743)
                     /locus_tag="VF954_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530716.1"
                     /translation="MPSTRTGQCPHGFPAGECMICQTLDPKAAIKPSSVLAPGSQARP
                     ARGRRPRRIGLSAAGVLVLVVLALVAVWFAWHLVWAVIHVVELVAVGAACGWAGWKLG
                     VGHGRRLERRGR"
     gene            complement(1743..2360)
                     /locus_tag="VF954_06245"
     CDS             complement(1743..2360)
                     /locus_tag="VF954_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9530717.1"
                     /translation="MSTPAAPHAVRKATYADVDRLAGALARAFDDDPVMTHLWPDAAR
                     RRAVLPAFFGAELHKVHLPLDEVWITHDLASGALWDPPGRWRLGIGGQIRLLPTMIRL
                     FGAGLARASATLAVAESKHPKVPEHYYLAVLGTDTERQGQGLGSAALAPVLEKCDLQG
                     VPAYLESSKETNVPFYRRHGFEVSEEFRLGRNGPPIWGMWREPAG"
     gene            2464..3705
                     /locus_tag="VF954_06250"
     CDS             2464..3705
                     /locus_tag="VF954_06250"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX9530718.1"
                     /translation="MTTLADLGARARAAAVELAAAPTSAKSSGLSAAADLLAQHEAEI
                     LHANADDVERAEASGTSATLVDRLRLDPARVAAMADGLRQVAALPDPVGEVVDGWVRP
                     NGLRIRRVRVPLGVIGVIYESRPNVTSDAAGLCVKSGNAAFLRGSSAAIRSNIAVAAA
                     LREGLAKAGLPEDAVVLVEDTSRESATEFMRLRGIIDCLVPRGGPSLIAAILEHATVP
                     YVIDGDGNCHVYVDESADLDMASTIVVNAKTQRPGVCNAAESLLVHAAVATDFLPKVA
                     EAMPEVRLLGDRRARSILPRIEAATDEDFSKEFLDHVMSVAVVGSIEEAIAHIRRHGT
                     GHTEAIVTRDLAASERFARDVDAAVVMVNASTRFTDGEQFGFGAEIGNSTQKLHARGP
                     MGLRELTAVKYIVHGDGHVRH"
     gene            3717..4643
                     /locus_tag="VF954_06255"
     CDS             3717..4643
                     /locus_tag="VF954_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9530719.1"
                     /translation="MTNTTELRTTIGGGEPRFASVPAVREGLRAAQYLADEGIAGVVY
                     LADRLGKPVLIEGPAGTGKTELAKSVARLTGSRLIRLQCYEGLDESKALYEWNYKKQL
                     LRIQAQRVAEAEGGSWAEIEEDIFSEQFLLTRPLLEAIRAEEPVVLLIDEVDRVEVET
                     EALLLEILSDYQVSIPELGTVSATQIPLVFLTSNNTRELSEALKRRCLYLHIDYPSLE
                     REKEIVLARVPDVTEHLADQIVRIVRSLRALDLKKHPSVSETLDWARTLVLLGVDSID
                     AGQAKETLNILLKYQSDIAKATKELSADAPTR"
     gene            4648..6075
                     /locus_tag="VF954_06260"
     CDS             4648..6075
                     /locus_tag="VF954_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9530720.1"
                     /translation="MLDLLAGFIQELRSAGLPVSLTENLDAMEAVKHIPIEDREAFKY
                     ALAATLVKNNAHWKAFETVFEVYFSRRGPELDTEDGGDQGDELAKLLQKMAAEAREGG
                     GAMDAMTPEQLAEMLFKAMLNADEATMKAVARQAVTRFSGMEPGRPVGGTYYLYRTLR
                     NLDLDRLMERLMEAAAAEAEGGLTPLEERLARDEHEARIDRLRKEVEAEIRRRLVADR
                     GVEAMARTLRKPLPEEIEFMHATRDELASLRRAIQPLTRKLAVRLARKRRHGRKGPLD
                     FRNTVRHSLSYGGVPAEPKFRFPRPAKPEIVVIADISGSVAAFARFTLQFVYAISSQF
                     SRVRAFVFIDGIDEVTSFFEGVEDITEAIHRVNTEADVVWVDGHSDYGHAFERFWERW
                     GRDIGPKSSVLLLGDARNNYHASQAWVVKELRSRARHVYWLNPEPRSYWDTGDSIVSE
                     YATFCDGAFECRNLKQLERFVENLA"
     gene            6120..6752
                     /locus_tag="VF954_06265"
     CDS             6120..6752
                     /locus_tag="VF954_06265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9530721.1"
                     /translation="MIRHGEAVCNVAGIIGGPRGCTGLSPLGVSQAVRLRGRLERTGE
                     LASASALVTSALPRAQQTASVIGPAVGDGSLVPAEDCDVCELHPGPEIDGLSWEEYSL
                     RFGGGSGGPLGPDQPIGPGGESWSVFRSRVSAALDRLAEDFERQTVVVACHGGVVVAA
                     MSHFLALPPPGERAGLEPDHTSLTEFARGAEGPWRLARYNDAAHLLDWRP"
     gene            complement(6830..7669)
                     /locus_tag="VF954_06270"
     CDS             complement(6830..7669)
                     /locus_tag="VF954_06270"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9530722.1"
                     /translation="MTSLAERLGYPADAKLLIINCDDLGSCHAANVGTFDALRRGMAT
                     SASLMVPCPWAREAASRYRGEDVGVHLTLNAEFDLYRWGPVSHAPSLFDGDGGFPRTV
                     EDLWDHADLDEVRRECRVQIERAIYWGFDVSHLDSHLATLQLRPEFFDVYLDLAVEFR
                     LPVRLSGASTERAIGFPFRALAADEGVVFPDHLVHVRGIGSRRPLERALMELPAGVTE
                     VYVRPAADTPELRCLAPDWSGRVDDHHLVTEERSLLGLIERAGIQLIGWRALRQLMRP
                     ARP"
BASE COUNT         1106 a         2896 c         2782 g         1069 t
ORIGIN      
        1 gggctgctgg tcgtgttcct cgccgtcgtc agcgtcggct ccatgcggct gttgacccgg
       61 gccgaccagc cgccgctcgt gcacgccgcc ggcgccctgt gcctcggtgt cctggtcccg
      121 ctcgtgaccg ctcccttcct caccaccacc ctgcgctggc ccgccctggt cggcatcgcc
      181 gccggcgtgg tgtgcgagca gatggatgcc caaggcgacg gcgcttccac cccggtgtga
      241 ggcgccggca cgtcaccttc gtggaccaca ccgcccagct gagcggcggc cagctggcga
      301 tggcccgcct gatcggcggc ctgcacgagc aggtcgacgt ccaggtggtg ctcggcgagg
      361 acggcccgct ggtccacgcc ctccaggtcg ccggcgcgga cgtcgtcgtg atgccgatcg
      421 accgcagcgc cgccacgctg gctcgccgcg acgtcgccct acggtcgctg cccctcgtgc
      481 cggcggccaa ggccgccgcc cacggctggg agctggctcg ccatctccgg cgtacccggc
      541 ccgacgtcgt gcacctcaac accctcaagg ctctcttcta cggcggcgtt gccgcccggc
      601 tggcgggtcg acccgccgtc tggcacgccc gcgaccggat cacgcccgac taccttcccg
      661 ccggggcggt ggccgtcacc cggctggcgg cccgtacgct cccgtccttc gtgatcgcca
      721 actcggtcgc aaccctgtcg acgctgcccc tgcggcgccc ccacgccgtc gtgcccagcc
      781 cggtcgacgc cgccgccttc gtcaccgccg cgccgccgcc ggggcccctc gccgtcggca
      841 tggtcgggcg catcgccccg tggaagggac aggacgtcct cctccgggcg ttcgccctgg
      901 cgttccccgg cggcgacgcc agcgtccgcc tcatcggtgc ccccctcttc ggcgaagagg
      961 cctacgagcg acgcctccac gacctggccg acgagctcgg catcgccggg cgggtggcgt
     1021 tcggcggctt ctccgacgac gtgcccggcg agctgggccg gctgcacgtc ctcgtgcacg
     1081 cctcggtgct gcccgagccg ttcggccagg tggtggtcga ggggatggcg gcgggcctcc
     1141 cggttgtggc cacccgggcc ggcgggccgg cggagatcgt caccgaaggc cgggacgggc
     1201 tgctggtggc gccgggcgac gcggccgagc tggccggcgc cctgagacgc ctcgccctcg
     1261 acgccgacct ccgcagtcgg ctgggagccg ccgcccggga gaccgcgggc cggttccgcg
     1321 tcgaggcggt ggccggccag gtgatgggga tctacgccgc cctcgcccgg tgagcccggt
     1381 ttcccccctg agtccgggtc acctcccccg gcgctcgagc cgcctgccat ggcccacgcc
     1441 aagcttccag cccgcccagc cgcaggccgc gcccacggcg accagctcga cgacgtggat
     1501 caccgcccac accagatgcc atgcgaacca caccgcgacc agcgccagaa ccaccaggac
     1561 caacacgccg gcggcggaga gcccgatcct gcgggggcga cgcccccgtg cgggacgagc
     1621 ctgcgagccc ggtgccagga ctgaagacgg cttgatcgcc gccttgggat cgagggtctg
     1681 gcagatcatg cactcgccgg cggggaagcc gtgcgggcac tgcccggtcc gggtggaggg
     1741 catcagcctg cgggctcccg ccacatgccc cagatgggcg ggccgttgcg gccgagccgg
     1801 aactcctcgc tcacctcgaa gccgtggcgg cggtagaagg gcacgttggt ctccttggac
     1861 gactcgaggt aggcggggac gccttggagg tcgcacttct cgagcaccgg ggccagggcg
     1921 gccgagccca ggccctggcc ctgccgctcc gtgtccgtcc ccagcaccgc caggtagtag
     1981 tgctccggca ccttggggtg cttggactcg gccaccgcga gggtggcgct cgcccgggcc
     2041 agcccggcac cgaagagccg gatcatcgtc ggcagcagcc ggatctggcc gccgatcccg
     2101 agcctccagc gcccgggcgg gtcccagagc gccccggagg ccaggtcgtg ggtgatccag
     2161 acctcgtcca gcggaaggtg gaccttgtgc agctcggcgc cgaagaaggc cggcaggacg
     2221 gctcgccgcc tggcggcgtc gggccagagg tgggtcatca ccgggtcgtc gtcgaaggcc
     2281 cgggccaggg cgccggccag ccggtccacg tcggcgtagg tcgccttgcg cacggcgtga
     2341 ggcgcggcgg gggtgctcat cggcaaccag tctgccccga cgcggcgcca ccgcgccgca
     2401 ctgccgccgg cccgccgggg attggactag accgaccggt caagtctcgg ggagacttgg
     2461 cgggtgacga cgctggccga cctcggggcg cgggcgcggg ccgccgcggt cgagctggcc
     2521 gccgcgccca ccagcgccaa gagctccggc ctctccgccg ccgccgacct cctggcgcaa
     2581 cacgaggctg agatcctcca cgccaacgcc gacgacgtcg agcgggcgga ggcctcaggc
     2641 accagcgcca ccctcgtcga ccggctgcgg ctggaccccg cccgggtggc ggccatggcc
     2701 gacggcctcc gccaggtcgc agccctgcca gacccggtgg gcgaggtggt ggacggctgg
     2761 gtccgcccca acgggctgcg aatccggcgc gtgcgcgtcc cgctgggcgt gatcggcgtc
     2821 atctacgaga gccggcccaa cgtgaccagc gacgcggccg gcctctgcgt caagtcgggc
     2881 aacgccgcct tcctgcgggg ctcctccgcg gccatccgct ccaacatcgc cgtggccgcc
     2941 gccctgcggg aaggcctggc caaggccggg ctgcccgagg acgccgtcgt gctcgtcgag
     3001 gacaccagcc gagagtcggc caccgagttc atgcgcctgc gcggcatcat cgactgcctc
     3061 gtgccgcgcg gggggccgtc gctcatagcg gccatcctgg agcacgccac cgtgccctat
     3121 gtcatcgacg gcgacggcaa ctgccacgtg tacgtggacg agagcgcgga tctcgacatg
     3181 gcgagcacca tcgtcgtcaa cgccaagacc cagcggcccg gcgtgtgcaa cgcggccgag
     3241 tcgctgctgg tccacgccgc ggtcgccacc gacttcctgc cgaaggtcgc cgaggccatg
     3301 cccgaggtgc ggctgctcgg cgaccggcgc gcccggtcga tcctgccgcg catcgaggcg
     3361 gccaccgacg aggatttctc caaggagttc ctcgaccacg tcatgtccgt cgcggtggtc
     3421 ggcagcatcg aggaggccat cgcccacatc cgccggcatg gcacggggca caccgaggcc
     3481 atcgtgacgc gggacctggc cgcctctgag cgcttcgcgc gggacgtcga cgccgccgtc
     3541 gtcatggtca acgccagcac ccgcttcacc gacggcgagc agttcggctt cggcgccgag
     3601 atcggcaaca gcacccagaa gctccatgcc cggggcccga tgggccttcg tgagctcacc
     3661 gcggtgaagt acatcgtcca cggcgacggc cacgtccgcc actgaaggag ctccttgtga
     3721 ccaacaccac cgagctgcgc acgaccatcg gcggcgggga gccccggttt gcctccgtcc
     3781 ccgccgtccg ggagggtctg cgcgccgcgc agtacctcgc cgacgagggc atcgccggcg
     3841 tcgtttacct cgcagaccgg ctgggcaagc ccgtgctgat cgagggcccc gccggcacgg
     3901 gcaagaccga gctggccaag agcgtggccc gcctcacggg ctcccgcctg atccggctcc
     3961 agtgctacga gggcctcgac gagtccaagg cgctgtacga gtggaactac aagaagcagc
     4021 tgctgcgcat ccaggcccag cgggtcgccg aggccgaggg cgggagctgg gcggagatcg
     4081 aggaggacat cttcagcgag cagttcctgc tcacccgccc gctgctcgag gccatccggg
     4141 ccgaggagcc ggtggtcctg ctcatcgacg aggtggaccg ggtggaggtc gagaccgagg
     4201 ccctgctgct ggagatcctg tcggactacc aggtctccat ccccgagctc ggcacggtgt
     4261 cggccaccca gataccgctg gtgttcctga cgtccaacaa cacccgcgag ctgtccgagg
     4321 ccctcaagcg gcgctgcctc tacctgcaca tcgactaccc gagcctggag cgggagaagg
     4381 agatcgtcct cgccagggtc cctgacgtga ccgagcacct cgccgaccag atagtgcgga
     4441 tcgtgcgcag cctgcgggcg ctggacctga agaagcaccc gtcggtgtcc gagacgctcg
     4501 actgggcccg gaccctggtg ctgctcggcg tcgactccat cgacgccggc caggccaagg
     4561 agaccttgaa catcctgctc aagtaccagt cggacatcgc caaggccacc aaggagctct
     4621 cggccgacgc ccccacgagg tagtcccgtg ctcgatctcc tcgccggctt catccaagag
     4681 ctgcgctccg ccggcctccc ggtatcgctc accgagaacc tggacgccat ggaggccgtc
     4741 aagcacatcc ccatcgagga ccgcgaggcg ttcaagtacg ccctggcggc cacgctggtg
     4801 aagaacaacg cccactggaa ggcattcgag accgtcttcg aggtctactt ctcgcggcgc
     4861 ggaccggagc tcgacacgga ggacggtggc gaccagggcg acgagctggc gaagctgctt
     4921 cagaagatgg cggccgaagc ccgcgaaggc ggcggggcca tggacgccat gacgcccgag
     4981 cagttggccg agatgctctt caaggccatg ctcaacgccg acgaggcgac catgaaggcc
     5041 gtcgcccggc aggccgtcac ccgcttctcg gggatggagc cgggccggcc ggtcggcggc
     5101 acctactacc tgtaccggac gctgcggaat ctcgacctcg accgcctgat ggagcgcctc
     5161 atggaggcgg ccgccgcgga ggccgagggc gggctgacgc cgctcgagga gcggctggcg
     5221 cgcgacgagc acgaggcgcg catcgaccgg ctccgcaagg aggtcgaagc cgagatccgc
     5281 cgccggctgg tggccgaccg gggcgtcgag gccatggccc ggacgctgcg gaagccgctg
     5341 ccggaagaga tcgagttcat gcacgccacc cgcgacgagc tggcatccct gcgtcgggcc
     5401 atccagcccc tgacccggaa gctggccgtg cgcctggccc ggaagcgccg gcacggccgg
     5461 aaggggccgc tcgatttccg gaacacggtg cgccattcgc tgtcctacgg cggcgtgccg
     5521 gccgagccca agttccgctt cccgcggccg gccaagcccg agatcgtcgt gatcgccgac
     5581 atctccggct cggtggcggc cttcgcccgc ttcacgctcc agttcgtgta cgccatctcg
     5641 tcgcagttct cgcgggtgcg tgcgttcgtg ttcatcgacg gcatcgacga ggtgacctcg
     5701 ttcttcgagg gcgtcgagga catcaccgag gccatccacc gggtcaacac cgaggccgac
     5761 gtcgtctggg tcgacggcca ctcggactac gggcacgcct tcgagagatt ctgggagcgc
     5821 tggggccgcg atatcgggcc caagagctcc gtgctgctgc tgggcgacgc ccgcaacaac
     5881 taccacgcct cgcaggcgtg ggtggtcaag gagctgcgct cccgcgcccg ccacgtgtac
     5941 tggctcaacc ccgagccccg ctcctactgg gacacgggcg actcgatcgt ctcggagtac
     6001 gccaccttct gcgacggcgc cttcgagtgc cgcaacctca agcagctcga gcgcttcgta
     6061 gagaacctgg cttgagctcc ggggcatctt cgggcccggg cggtgagacc cgactggtct
     6121 tgatccggca cggcgaggcg gtgtgcaacg tggccggcat catcggcggg ccacggggct
     6181 gtaccggcct gtcgccgctc ggcgtcagcc aggcagtgcg gttgcggggg cgcctggagc
     6241 gcacgggcga gctggcgtcg gcctcggctt tggtcacctc ggcgctgccc cgcgcccagc
     6301 agacggcgtc ggtcatcgga cccgcagtcg gcgacggcag cctggtgccg gccgaggact
     6361 gcgacgtctg cgagctgcac cccgggcccg agatcgacgg gctgtcgtgg gaggagtaca
     6421 gcctgcggtt cggcggcggc tcgggcggcc cgctcggacc cgaccagccc atcggtccgg
     6481 gaggggaaag ctggagcgtc tttcgttcac gggtgagcgc cgcgctggac cgcctggccg
     6541 aggacttcga gcgccagacc gtggtggtcg cctgccacgg tggggtcgtg gtggcggcga
     6601 tgtcccactt cctcgccctc ccgccgccgg gcgagcgggc cgggctggag ccggaccaca
     6661 catctctaac ggagttcgcc cgtggcgcgg aggggccgtg gcggctggcc cgctacaacg
     6721 acgccgctca tctcttggac tggcggccgt aggcagaggc cgcggccggc ccggctgcgg
     6781 gctgggcagg agaggcagcg gagctcggcg aggacctgcc cgagactttc tatgggcggg
     6841 cgggccgcat gagctggcga agggcgcgcc agccgatcag ctggatgccg gcgcgctcga
     6901 tcaggccgag gagtgagcgc tcctcggtga cgaggtggtg gtcgtcgacc cgcccggacc
     6961 agtccggggc caggcagcgc agctccggcg tgtccgccgc gggacggacg tacacctccg
     7021 tcacgccggc cggcagctcc atcagggccc gctccagcgg ccgcctgctg ccgatgcccc
     7081 ggacgtggac cagatgatcg ggaaagacca caccctcgtc ggcggccaga gcgcggaacg
     7141 ggaacccgat ggcccgttcg gtcgacgcgc ccgacaaccg cacgggcagc cggaactcga
     7201 ccgcgaggtc gaggtacacg tcgaagaact ccggccgcag ctgcaacgtc gccaggtgcg
     7261 agtcgaggtg gctcacgtcg aagccccagt agatcgcccg ctcgatctgc acccggcact
     7321 cgcggcggac ctcgtcgagg tcggcgtggt cccagaggtc ctcgacggtg cggggaaagc
     7381 ctccgtcgcc gtcgaacagg gagggcgcat gggagacggg gccccaccgg tacaggtcga
     7441 actcggcgtt gagcgtcagg tgcacgccca cgtcctcgcc ccggtaccgg gacgcggcct
     7501 cccgcgccca ggggcagggg accataaggc tggcgctcgt ggccatcccc cgccgcaggg
     7561 cgtcgaaggt gccgacattg gccgcatggc acgagcccag gtcgtcacag ttgatgatca
     7621 gcagcttggc gtcggccggg tacccgaggc gttccgccag gctggtcatg accgcgacgg
     7681 tactcgccct cccccgggcg gtcggatagc tcacgcccgg atggcgctcc aggtccttca
     7741 tgtttccgtc ccggcccccg gtgtcaccga tgtcacgggg cgacgatctg gaccgaaccc
     7801 atgctgcccc ggatcgtgag gctgccgtcg ccggccccga tcgtggcgtc acg
//
LOCUS       DASWGG010000345         4203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2879186, whole genome shotgun sequence.
ACCESSION   DASWGG010000345 DASWGG010000000
VERSION     DASWGG010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2879186"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..818)
                     /gene="pstB"
                     /locus_tag="VF954_06275"
     CDS             complement(42..818)
                     /gene="pstB"
                     /locus_tag="VF954_06275"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX9530723.1"
                     /translation="MSKRIEVQHLSVWYGAQQAVDDVSLVMEPKQVTALIGPSGCGKS
                     TLIRAINRMHDNTLGARVAGRVLLDDSDLYERGVNPTDVRRTIGMVFQRANPFPTMSI
                     RDNVAAGLRLNGLRNKARVEEIVERSLRRAGLWEEVSDRLNRAGGSLSGGQQQRLCIA
                     RAIAVEPEVLLMDEPCAALDPVSTLRIEDLIADLKDRYTIIIVTHNMQQAARVSDVTA
                     FFNIAGAGEPGRLVEVGPTAKVFTTPDDERTEAYITGRFG"
     gene            complement(835..1869)
                     /gene="pstA"
                     /locus_tag="VF954_06280"
     CDS             complement(835..1869)
                     /gene="pstA"
                     /locus_tag="VF954_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX9530724.1"
                     /translation="MAPSPFGLARRRLGRGQLLALAGACVAGTVALGATRALGWSLSL
                     VLLFGAYLVVSTTAAWRLEGRRPAVNRLACTLLGAAVVVTLIPLLAVLGYTLAKGGKA
                     LDGHFLTHSMRGVGPLEASGGEYHAVIGTLEQVLVASVISIPLGLLTAIFVVEYGRGR
                     MALLVRFFVDVMTGVPSIVAGLFVFAFWVIGLHKGFSGFAAGMALAVLMLPVVARASE
                     EMLRLVPKELRDASYALGIPKWRTITSVVLPAASAGITTGVILAVARVTGETAPLLLT
                     AFGFDSVRTGVFRGPQSALPLYVFSQASSAFDVAVHRAWAGALTLILIVLVLSVVARI
                     VTRRNTLSAM"
     gene            complement(1883..2866)
                     /gene="pstC"
                     /locus_tag="VF954_06285"
     CDS             complement(1883..2866)
                     /gene="pstC"
                     /locus_tag="VF954_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX9530725.1"
                     /translation="MATASPLPVAPGAPPELTRRSPLGDRVFRAIAYAASMFVLVLIA
                     AIAVFLLAKSWPAFRQEGLGFFTRRQWFPDQPHPDFGVAALAVGTVVSSVIALLIAVP
                     VALGSALFATELAPPSLGRWTGYLVDLLAAVPSVVYGLWGVIFLVPKLVPLERNLDHH
                     LGFIPLFHSPNHTYGRSVFAASVVLAIMILPIIAAISREVFRQVPRANREAALALGAT
                     RWEMIRMSVLPYSRGGIIGAVMLGLGRALGETIAIALVLAVAFNVNLHIFSPGGNTIA
                     ANIATKFGEAGALGRDGLIASGVVLFGITLVVNMAARAIVYRSGRVAEGRP"
     gene            complement(2943..4034)
                     /gene="pstS"
                     /locus_tag="VF954_06290"
     CDS             complement(2943..4034)
                     /gene="pstS"
                     /locus_tag="VF954_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX9530726.1"
                     /translation="MTTTRWLKAAAGAAAVMTATAACSSSSKPASSGTTTTAAPKVQC
                     ATGSVSAAGSTFVQNLAQQWIKDYEAACPGATVNYQGVGSGAGVQQLLAGTVDFAGSD
                     FPLGTADMATAQSKGGAVVQVPWAAGGIALEYKLNGVTDLKLSPDTIASIFAGKVTKW
                     DDAAIKADNAGAALPSEGITVVHRSDGSGTTNALTSYLTAVSPGIWTFGSDKTWKSPA
                     GQGAKGSDQVTAVVKQTEGAIGYAELSFAKGNSLSVAAVKNPSGQFVSPDSTSVKAAL
                     AEATVPGTGLVKLNFMPSDAGAYPISIATWAIVYQKPSDPAKAKLIQSFLTYASTTGQ
                     SAADGLYYTPLPAMFGSTDTALIASIQTS"
BASE COUNT          660 a         1427 c         1532 g          584 t
ORIGIN      
        1 ctggtccacg tcctccagca cccggcgggg cacgggccgc ctcaaccgaa ccggcccgtg
       61 atgtaggcct cggtgcgctc gtcgtcgggg gtggtgaaca ccttggccgt agggcccacc
      121 tcgaccaggc ggccgggctc gccggcgccg gcgatgttga agaacgcggt cacgtcgctg
      181 acacgggccg cctgctgcat gttgtgggtg acgatgatga tggtgtagcg gtccttcagg
      241 tcggcgatca ggtcctcgat ccgtagggtc gaaaccgggt ccagtgcggc gcacggctcg
      301 tccatgagga gcacctcggg ctcgacggcg atggctcggg cgatgcacag gcgttgctgc
      361 tgcccacccg agaggctgcc gccggcgcgg ttgagccggt ccgagacctc ctcccacagg
      421 ccggcgcgtc gcaaggaacg ttcgacgatc tcctcgaccc gcgccttgtt gcgcaagccg
      481 ttgagccgca acccggcggc cacgttgtcg cggatcgaca tggtggggaa ggggttggcg
      541 cgctggaaga ccatgccgat ggtcctgcgg acgtccgtcg ggttgacgcc ccgttcgtag
      601 aggtcgctgt cgtccagcag cacccgcccc gcgacgcggg ctccgagggt gttgtcgtgc
      661 atgcggttga tggcccggat cagcgtcgac ttgccgcaac cggaagggcc tatcagggcc
      721 gtcacctgct tgggctccat caccaggctg acgtcgtcga cggcctgttg ggctccatac
      781 cacacgctca ggtgctggac ctcgatgcgt tttgacatca cacgggccct gacatcacat
      841 cgcggagagg gtgttgcggc gtgtcacgat ccgggccacg acgctgagga ccagcacgat
      901 caggatcagt gtcagggcgc cggcccacgc cctgtgcacg gccacgtcga aggccgagct
      961 ggcctgggag aacacgtata gcggcagggc cgactggggg cccctgaaca cgcctgtgcg
     1021 cacgctgtcg aagccgaagg cggtgagcag gagcggggcg gtctcgccgg tcacccgggc
     1081 gacggcgagg atgactcccg tagtgatccc ggccgacgcg gcggggagca cgaccgaggt
     1141 gatcgtgcgc cacttcggga tcccgagggc gtagctggcg tcgcgcagct ccttgggcac
     1201 gaggcgcagc atctcctcgc tggcccgggc caccaccggg agcatgagca cggcgagggc
     1261 catgcccgcg gcgaagccgg agaacccctt gtgcaggccg atcacccaga aggcgaagac
     1321 gaacaggccg gccacgatgc tcggcacccc ggtcatcacg tccacgaaga agcggacgag
     1381 caaggccatc ctcccccggc cgtactccac cacgaagatg gccgtgagca ggcccagggg
     1441 gatggagatg acgctcgcca ccaggacctg ctcgagggtc ccgatcacgg cgtggtactc
     1501 gccgccgctc gcctccaggg gcccgacccc gcgcatcgaa tgggtgagga agtggccgtc
     1561 gagggccttg ccccccttgg ccagggtgta gccgagcacg gcgagcaggg ggatgagggt
     1621 caccaccacc gccgccccca ggagggtgca ggccaggcgg ttgaccgcgg ggcggcgtcc
     1681 ctccagccgc caggcggccg tcgtggacac cacgaggtat gcgccgaaga gcagcacgag
     1741 cgagaggctc cagcccagcg ccctggtggc gccgagcgcg accgtgccgg ccacgcacgc
     1801 ccccgccagg gcgagcagct ggccccgccc gagccggcgc cgggccagcc cgaagggcga
     1861 gggtgccact ccggtcccca cgtcagggcc tgccctcggc tacccgcccg ctgcggtaca
     1921 cgatggcccg ggcggccatg ttgaccacca gggtgatgcc gaagagcacc acgcccgagg
     1981 cgatcagccc gtcccgcccg agcgctccgg cctcgccgaa cttggtggcg atgttggccg
     2041 cgatggtgtt gcccccgggg ctgaagatgt ggaggttgac gttgaacgcg accgccagca
     2101 cgagggcgat ggcgatggtc tcgcccaggg cccggcccag accgagcatg acggcgccga
     2161 tgatgccgcc ccggctgtag ggcagcaccg acattcggat catctcccac cgggtggctc
     2221 ccagcgccag ggcggcctcg cggttggccc gcgggacctg tcggaagacc tcccgcgaga
     2281 tggccgcgat gatcggcagg atcatgatgg ccagcacgac cgacgcggcg aagaccgacc
     2341 ggccgtaggt gtggttcggg ctgtggaaga gagggatgaa acccagatgg tggtcgaggt
     2401 tgcgctccag cggcacgagc ttggggacga ggaagatgac gccccacagc ccgtagacca
     2461 cgctcggcac ggcggccagg agatcgacga ggtacccggt ccaccgcccg agggacggcg
     2521 gcgccagctc ggtcgcgaac agagccgagc ccagggcgac cgggacggcg atcaacagcg
     2581 cgatgacgct cgagacgacc gtgccgacgg cgagggcggc gacaccgaaa tcggggtggg
     2641 gctggtcggg gaaccactgc cggcgggtga agaagcccag accctcctgg cgaaaggccg
     2701 gccacgactt ggccaggagg aacaccgcga tggctgcgat cagcacgagc acgaacatgc
     2761 tggccgcgta ggcgatcgca cggaagaccc ggtcgcccag cggggagcgg cgggtcagct
     2821 cgggcggcgc accgggtgcg actggtaggg gggacgcggt cgccatgttt gctccaacgg
     2881 gcgggagccc ggcggccgag cctagccgcc gccgggatgg cgccgggact ggtccggccc
     2941 gctcaggaag tctggatgga cgcgatcagg gcggtgtccg tggatccgaa catcgccggc
     3001 agcggcgtgt agtacagccc gtcggctgcg ctctgaccgg tcgtggacgc ataggtcagg
     3061 aacgactgga tgagcttggc cttggccggg tcgctgggct tctggtacac gatggcccag
     3121 gtggcgatgg agatcgggta ggccccggcg tcgctcggca tgaagttgag cttgaccagg
     3181 cccgtccccg gcacggtcgc ctcggccagg gcggccttca ccgaggtgct gtcggggctg
     3241 acgaactgcc cggaggggtt cttcaccgcc gccacgctca ggctgttgcc cttggcgaag
     3301 gacagctcgg cgtagccgat ggcgccctcc gtctgcttga ccaccgcggt cacctgatcg
     3361 gaccccttgg ccccctggcc cgccggcgac ttccaggtct tgtccgagcc gaaggtccag
     3421 atgccaggtg acacggccgt gaggtacgag gtcaaggcgt tggtcgtgcc ggagccgtcc
     3481 gagcggtgca cgacggttat gccctcgctc ggcagggcgg ccccggcgtt gtcggccttg
     3541 atggcggcgt cgtcccactt ggtgaccttg ccggcgaaga tggaggcgat ggtgtccggc
     3601 gacagcttca ggtccgtgac cccgttgagc ttgtactcca gggcgatgcc gccggccgcc
     3661 caggggacct gcacgaccgc gccacccttc gactgggccg tcgccatgtc ggccgtgccg
     3721 agcgggaagt cgctgccggc gaaatcgacc gtgccggcca gcagctgctg gacacccgct
     3781 cccgagccca cgccctgata gttgacggtg gcgcccgggc acgcggcctc gtagtccttg
     3841 atccactgct gggccaggtt ctggacgaac gtcgagcccg cggccgacac ggagccggtg
     3901 gcgcactgga ccttcggggc cgccgtcgtg gtcgtgccgc tcgaggccgg cttgctgctc
     3961 gagctgcacg ctgcggtcgc ggtcatcacg gccgccgccc cggccgccgc cttgagccat
     4021 ctcgtcgtcg tcatgggcgc gaccctaagg tcgcccggca aacctgaccc aacgccaacc
     4081 tgaacggcga gttaacagcc gacgaacaat cggcggcgac gcggccccgc attcccggct
     4141 tcgacccgtc cggcggacta cggtcggcac gtggcggtgc gcatatggcc ataggcgacg
     4201 gcg
//
LOCUS       DASWGG010000346         3698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_468925, whole genome shotgun sequence.
ACCESSION   DASWGG010000346 DASWGG010000000
VERSION     DASWGG010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468925"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="VF954_06295"
     CDS             <1..1467
                     /locus_tag="VF954_06295"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEX9530727.1"
                     /translation="PVPSLRATTAAALGIDLASASRAEVDAVEQSCREKVEALAAAGW
                     VAAPGPAGPDDPTLGWVASRLVPALGRTADELDNIVAALEGRFVPPGPSGAPSRGAAH
                     VLPTGRNFYSVDPKALPTRLAWDVGRRLADGVVGRYVAETGAPPRTIGLVLWGTAAMR
                     TGGDDAAQALALLGVRPVWDAASGRVVGVEAVSLEELGRPRVDVTLRISGFFRDAFPQ
                     LVALLDDAVRLAAAQDEDSEHNPVRAAGGSDARIFGPPPGGYGSGILAVIEQGSWRSD
                     ADLGEVYLAWSGFAYTRAERGRAAPEEMRRRFSAIDVAVKNQDNREHDIFDSDDYLQD
                     HGGMVAAVRALTGRSPRAWFGDTADPAHPQVRALAEEAARVVRSRVLNPKWIGAMRGH
                     GYKGAFELVATVDYLFGYDATTGVVEDWMYERVTDSYVADTEMREFFRRSNPTALRAI
                     AERLLEAADRGLWDAPTEAARKTLVDAVLEVEGWEEGT"
     gene            1464..3356
                     /locus_tag="VF954_06300"
     CDS             1464..3356
                     /locus_tag="VF954_06300"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9530728.1"
                     /translation="MSSGAFPFGAVVGQADAQLALLLAAVDPGIGGVLLRGDKGSAKS
                     TLARGLAGLLPPGAPFVELPVGATEDRVVGGLDLGEAFAGAASLRPGLLSAAHGGVLY
                     VDEVNLLPDHLVDLLLDAAASGVHHVERDGLSHTAPARFVLVGSMNPEEGELRPQLLD
                     RFGLAVEVRSPREPAWRAAAVRNRLEFDAGRLGARVSREDEELAARLAVARPASLPDD
                     VIAHACRLAVAAGAEGLRADLVLCRAAAALAGWEGRDLTATEDVNRVAALALAHRRRR
                     RPFDPPTMAAEDLAKALSEATQSAAPPAAPPAVPPAAGGPGPGPGGPTPRSKSGDAGL
                     RRSDSDAVSARWADLSHADLYDAGLLPALPIGAASPVGPARPEWTVGPEVRGPQVGER
                     VPGPGGPVAVAVAASVRASLRRRAAAPQWAVGSVGSVDSVGLVGDDLREPLRRRAQRQ
                     CTILVVDTSGSMGVTSRVEAATGAVLSLLADAYRRRHRVALVACRGMRADVVLPPTAS
                     VELARARLRDLPTGGATPLAEGIDAALAVARAVRRDDDHPTVVLLTDGRATSGADALG
                     RARAAARELAGEGIATLVLDAEDGPARLGIAAELADAAGGRCVPLAAVTADAVESAIR
                     AAETRG"
     gene            3353..>3698
                     /locus_tag="VF954_06305"
     CDS             3353..>3698
                     /locus_tag="VF954_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEX9530729.1"
                     /translation="MSQDALVLVGHGSRSAASDAEMLRLRDQVAAGLPKVAVDLGFLE
                     MSEPPAHVVVDEAIAAGARRVVVLPLVLFGAGHAKSDVPAIVVGARARHPEVDVRFGS
                     PLGVSHGLVAILG"
BASE COUNT          388 a         1389 c         1467 g          454 t
ORIGIN      
        1 ccggtgccgt cgctgcgggc cacgaccgcc gccgcgctcg gcatcgacct ggcgtcggcc
       61 agccgggccg aggtcgacgc cgtcgagcag agctgccggg agaaggtcga ggccctggcc
      121 gcggcgggct gggtggccgc gcccgggcct gcggggccgg acgaccccac cctgggctgg
      181 gtggcgagcc gcctcgtccc cgccctgggc cggaccgccg acgagctgga caacatcgtc
      241 gccgccctcg agggccggtt cgtcccgccg ggcccgagcg gggcgccgag ccggggcgcg
      301 gcccacgtcc tgccgacggg ccggaacttc tactcggtcg accccaaggc cctgcccacc
      361 cggctggcct gggacgtggg ccggcgcctg gccgacggcg tggtcgggcg ctacgtcgcc
      421 gagacgggcg ccccgccccg caccatcggc ctggtgctgt ggggcacggc ggccatgcgc
      481 accggcggcg acgacgccgc ccaggccctg gccctgctgg gcgtgcggcc ggtgtgggac
      541 gccgcgtccg ggcgggtggt cggtgtcgaa gccgtctccc tggaggagct gggccggccc
      601 cgggtggacg tgaccctgcg tatctccggc ttcttccggg acgccttccc ccagctggta
      661 gccctgctgg acgacgccgt ccggctggcc gcggcgcagg acgaggacag cgagcacaac
      721 ccggtccggg ccgcgggcgg ctccgacgcc aggatcttcg ggcccccgcc gggcggctac
      781 ggctcgggca tcctggcggt catagagcag ggctcgtggc gctcggacgc cgacctgggc
      841 gaggtgtacc tggcgtggtc ggggttcgcg tacacccggg ccgagcgcgg ccgcgccgcc
      901 cccgaggaga tgcgccggcg tttctccgcg atcgacgtcg cggtcaagaa ccaggacaac
      961 cgcgagcacg acatcttcga cagcgacgac tacctccagg accacggcgg gatggtcgcg
     1021 gcggtccggg ccctcaccgg ccggtcgccg cgggcctggt tcggggacac cgccgacccg
     1081 gctcacccgc aggtgcgggc cctcgccgag gaggccgccc gggtggtgcg gtcccgggtg
     1141 ctcaacccga agtggatcgg cgccatgcgc gggcacgggt acaagggcgc cttcgagctg
     1201 gtggccacgg tggactacct gttcggctac gacgccacga ccggcgtggt cgaggactgg
     1261 atgtacgagc gggtgacgga cagctacgtg gccgacacgg agatgcgcga gttcttccgg
     1321 cgctccaacc cgaccgcgct gcgggccatc gccgagcggc tgctcgaggc cgccgaccgc
     1381 gggctgtggg atgcgccgac cgaagcggcc cgcaagacgc tggtggacgc cgtgctagag
     1441 gtggagggat gggaggaagg gacatgagct caggcgcgtt cccgttcggg gcggtcgtcg
     1501 gccaggccga cgcccagctg gcgctgcttc tggccgccgt cgacccgggc atcggcgggg
     1561 tgctgctgcg cggcgacaag ggctcggcca agtcgacgct cgcccggggg ctggccgggc
     1621 tgctgccgcc gggcgcaccg ttcgtggagc tgccggtggg cgccaccgag gaccgggtgg
     1681 tgggcgggct cgacctcggt gaggccttcg ccggcgcggc ctccctccgg cccggcctgc
     1741 tgagcgcggc gcacggcggc gtgctgtacg tcgacgaggt caacctcctc cccgaccacc
     1801 tcgtggacct cctgctcgac gcggccgcgt cgggcgtgca ccacgtcgag cgcgacgggc
     1861 tgagccacac ggccccggcc cggttcgtgc tcgtcggctc catgaacccg gaggagggcg
     1921 agctgcgccc ccagctgctc gaccggttcg gcctcgccgt ggaggtgcgt tcgccgcggg
     1981 agccggcgtg gcgcgccgcg gcggtgcgga accggctgga gttcgacgcc ggccggctcg
     2041 gcgctcgtgt ctctcgtgaa gacgaggagc tggccgcccg cctggccgtg gcgcggccgg
     2101 cgtcgctgcc cgacgacgtc atcgcccacg cctgccggct ggccgtggca gcaggcgccg
     2161 aggggctgcg tgccgacctg gtgctgtgca gagcggcagc cgccctggcc ggatgggagg
     2221 gccgggacct caccgccacg gaggacgtca accgggtcgc ggcgctggcg ctggcccacc
     2281 gccggcgccg ccggccgttc gatcccccga cgatggcagc cgaagacctg gccaaggcct
     2341 tgtcggaggc cacgcagagc gccgctcccc cggccgctcc ccccgccgtt cccccggccg
     2401 cgggcggtcc cggccccggc cccggtggac cgaccccccg atcgaagtcc ggggacgcgg
     2461 gattacggcg gtcggactcc gacgccgtgt cggccaggtg ggccgacctg tcccacgccg
     2521 acctgtacga cgccggcctg ctcccggccc tgccgatcgg cgcggcttcg ccggtcggcc
     2581 cggcccggcc ggagtggacg gtgggcccgg aggtgcgggg cccccaggtg ggagagcggg
     2641 tccccgggcc gggcggcccg gtcgcggtcg cggtcgcggc ctccgtgcgg gccagcttgc
     2701 gccggcgcgc ggccgcgccg cagtgggcag tgggctcggt gggctcggtg gactcggtgg
     2761 gcttggtggg cgacgatctc cgcgagcccc tccgtcggcg ggctcagcgg cagtgcacca
     2821 tcctcgtcgt cgacacgagc ggctcgatgg gggtcaccag tcgggtggag gcggccaccg
     2881 gcgcggtgct cagcctgctg gcggacgcct accgccgccg ccaccgggtc gccctggtgg
     2941 cctgccgggg catgcgggcc gacgtcgtgc tgccgccgac cgccagcgtc gagctggccc
     3001 gggcccggct gcgcgacctg ccgaccggtg gggccacccc gctcgccgag ggcatcgacg
     3061 ccgccctggc cgtggcgcgc gccgtgcggc gcgacgacga ccacccgacg gtcgtgctcc
     3121 tcaccgacgg gcgcgccacc tcgggagccg acgccctcgg ccgggcccgg gcggcggcgc
     3181 gcgagctcgc cggcgagggc atcgccacgc tggtcctcga cgccgaggac ggcccggcgc
     3241 gcctgggcat cgcggccgag ctggccgacg cggccggcgg ccggtgcgtg ccccttgcgg
     3301 ccgtgacggc cgatgccgtc gagtcggcca tccgggccgc ggagacgcgg gggtgagcca
     3361 ggacgcgttg gtgctggtgg gtcacgggtc ccgttcggcc gcgagcgacg ccgagatgct
     3421 ccggctgcga gaccaggtcg cggccgggct gcccaaggtg gccgtcgatc tcggcttcct
     3481 cgagatgagc gagccgccgg cccacgtcgt ggtcgacgag gcgatcgcgg caggagcgcg
     3541 ccgcgtcgtg gtcctgcccc tcgtcctgtt cggcgcgggg cacgccaaga gcgacgtccc
     3601 cgccatcgtg gtcggtgccc gggcccgcca cccggaggtc gacgtccgct tcggcagccc
     3661 gctgggcgtg tcccacgggc tcgtcgccat cctgggcc
//
LOCUS       DASWGG010000347         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1925049, whole genome shotgun sequence.
ACCESSION   DASWGG010000347 DASWGG010000000
VERSION     DASWGG010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925049"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="VF954_06310"
     CDS             <1..1144
                     /locus_tag="VF954_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX9530730.1"
                     /translation="VVFVAVLWAAAFTTASFSALNERELRRRRADMEALAEMAAALEA
                     LTDPVTIAEVLLERVCDALGFHRGLVLAGPPEGRLRLLAQRGLEGSPHDPAQPVEADP
                     RSVVNQALSSWGALVHDSLDVDADPWLVQLLGWPRRLMVAPLSGESRPVGVLVLDYPR
                     PAGARVPRRVAAMVERFASYGGLALRNAWLLAQLTRRAATDGLTGIANRRSFDTNLSR
                     EIGRARRSGDPVSLVMIDIDHFKGLNDEKGHQVGDGVLRRVAGLLHDHCRDFDTAARY
                     GGEEFALILPSCPADTGAATADRMRIMIAETESELGVTVSAGVASYPTDAEDAFSLIG
                     AADQALYLSKRSGRNRVTCAQEIGAAHQAAPRRSPLSEPDGTSVTS"
     gene            1337..1987
                     /locus_tag="VF954_06315"
     CDS             1337..1987
                     /locus_tag="VF954_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530731.1"
                     /translation="MNDDRPDGGSWADAADARQPGSDDAERRASAEVDALLADLRSVL
                     AEAGVEEAVMARSRERWLRRQAEEEATMSSLLIDAVERGGPVVVRTVAGRSHHGRLTA
                     VAADCCVLRTSAGTVTLLPLRAVTSLRHGPGRRHREAATSRRSPVAATFVGLLADLAG
                     ERPRVRLACDGNPDVLAGELRAAGADVLTLQQEADPPAIVYVRLASVTEVSLLGSG"
     gene            complement(1936..2163)
                     /locus_tag="VF954_06320"
     CDS             complement(1936..2163)
                     /locus_tag="VF954_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HEX9530732.1"
                     /translation="MSTKEAAERLGITLRTLYRFMDEGLIAAYKFGRVIRLKEQDVEG
                     FIESSRIAPGSLAHLYPEPKSETSVTEASRT"
     gene            complement(2215..>2425)
                     /locus_tag="VF954_06325"
     CDS             complement(2215..>2425)
                     /locus_tag="VF954_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530733.1"
                     /translation="RPPTGEVYLFSGWAPEGAPLSSAMVQRTGVTDGTASVVRGGAGY
                     CDRVEVRPDSKPVGGHLDAAGKETP"
BASE COUNT          334 a          881 c          842 g          368 t
ORIGIN      
        1 ggtcgtgttc gtagcggtgc tgtgggcggc ggccttcacg accgcgtcgt tctcggcgct
       61 caacgagcgg gagctgcgcc ggcgccgggc cgacatggag gcgctggccg agatggccgc
      121 cgccctcgag gccctgaccg atccggtcac catcgccgag gtcctcctcg agcgggtctg
      181 cgacgcgttg ggcttccacc ggggattggt gctggcgggc cctccggagg ggcgactgcg
      241 actgctggcg caacgcgggc tcgaggggtc gccgcacgac ccggcccagc ccgtggaggc
      301 cgaccctcgc tcagtcgtca accaggcgtt gtcgagctgg ggcgcgctgg tccatgacag
      361 cctcgacgtc gacgccgacc catggctggt gcagctgctc gggtggccac ggcggctcat
      421 ggtcgcgccg ctgtccggcg agagccggcc ggtgggcgtg ctcgtgctgg actacccacg
      481 accggccggc gcccgggtgc ctcgccgcgt ggcggccatg gtcgagcgct tcgcctccta
      541 cggtggtctc gccctgcgca acgcctggct gctggcccag ttgacccgcc gggccgccac
      601 cgacgggctg accgggatcg ccaaccggcg ctcgttcgac accaatctca gccgcgagat
      661 cggccgggcc cgccggtccg gcgatcccgt cagcctggtg atgatcgaca tcgaccattt
      721 caaggggttg aacgacgaga agggccatca ggtcggtgac ggcgtgctgc gccgcgtggc
      781 cggcctgctg cacgaccact gccgcgactt cgacaccgcc gcccgctacg gcggggagga
      841 gttcgccctg atcctgccga gctgtcccgc cgacaccggg gccgccaccg ccgatcggat
      901 gcggatcatg atcgccgaga cggagtcgga gctcggtgtg acggtgagcg ccggggtggc
      961 cagctatccc acggacgcgg aagacgcctt ctccctcatc ggcgcggccg accaggccct
     1021 gtacctgtcg aagcgctccg gtcgcaaccg ggtgacctgc gcccaggaga tcggcgccgc
     1081 ccaccaggct gccccccggc gctccccact gtccgagcca gacggaacct ccgtcacgtc
     1141 atagggggcc gccgacaccc gcccgcaccc cgccggcacc cgcgaccgac acagctccgg
     1201 taagggtcga acgcaaaact acttgggcgg tcgaagacca ttgccctagg tagtgacggc
     1261 caaagttctt gagaaccgcc caagtagttt tgcgcttgac cctagcgccc gccccgtgtc
     1321 gttagcctgc gcatcggtga acgacgatcg gccggacggg ggcagctggg ctgacgcggc
     1381 cgacgctcgc caaccggggt cggacgatgc ggagcgccgg gcctcggccg aggtcgacgc
     1441 cttgctcgcc gacctgcgca gcgtcctggc cgaggcgggc gtggaggagg ccgtgatggc
     1501 ccgcagccgt gagcgatggc tgcgacggca ggccgaggag gaggccacca tgagcagctt
     1561 gctcatcgat gcggtcgagc gcggcggccc cgtcgtggtc cggaccgtcg ccggccggtc
     1621 ccatcacggc cggctcaccg ccgtggcggc cgactgttgc gtgttgcgta cctccgccgg
     1681 cacggtcacg ttgctgccgt tgcgcgccgt aacctcgctc cgccacggtc ccggtcggag
     1741 gcatcgggaa gcggccacct cccggcggtc acccgtcgcg gcaaccttcg tgggcctgct
     1801 ggccgacctg gcaggcgaac gaccacgcgt tcgcctggcg tgcgacggta atcccgacgt
     1861 actcgccggg gagctgcgcg ccgccggcgc cgacgtcctc acgcttcagc aggaggccga
     1921 cccgccagcc atcgtctatg tgcgactggc ctcggtcacc gaggtctcgc tcttgggctc
     1981 cgggtagagg tgggccaggc tcccaggcgc gatccgggac gactcgatga agccctccac
     2041 gtcctgctcc ttgaggcgga tcacccggcc gaacttgtac gcggcgatca gcccttcgtc
     2101 catgaaccgg tacagcgtgc gaagggtgat gccaaggcgt tcggcggctt ctttggtgct
     2161 catccaacga atgggctcag tcatcggtgc ctatcctacc cacgccgtgt gacttcatgg
     2221 cgtttccttc ccggcagcgt cgaggtggcc gccaacaggt ttcgagtcgg gccggacctc
     2281 gacccgatcg cagtaccccg cccctccacg cacgacgctc gccgtgccgt ccgtgactcc
     2341 cgtccgttgc accatggcgc tggacaacgg cgcgccctct ggagcccacc cggagaagag
     2401 gtacacctca ccggtcgggg gtcgg
//
LOCUS       DASWGG010000348         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1925579, whole genome shotgun sequence.
ACCESSION   DASWGG010000348 DASWGG010000000
VERSION     DASWGG010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925579"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="VF954_06330"
     CDS             complement(<1..1601)
                     /locus_tag="VF954_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9530734.1"
                     /translation="MAQGSGDEAGRSTAIVLFTDLVGSTELRSRLGENAADVLRHQHD
                     ALVAGAVKASRGQVVKNLGDGVMATFVGASDAVGAAVAIQQAIGRHNRSGAAVLEVRI
                     GISVGDVVFEADDCFGTPVIEAARLCGAAAGGQILASELVRWLARSSEEKFTPIGSLE
                     LKGLPEPIPAVQVGWEPLPESSVPLPTFLTDIGRIFVGRDGELERLGQLWKEAMAGEL
                     RVALLAGEPGVGKTRLAAELARKAHDEGAAVLAGRCDEDLGVPYQPFVEALRHFVDHA
                     PALPGRLGRYGGELARLVPELSERVPGLPAPLRSDPETERYRLFDGVASWLAAASVDE
                     PVLLVLDDLQWAAKPTLMLLRHVVRAGGGRVLVLGIYRDSELTHEHPLLEVVPDLRRQ
                     GGVERLSLTGLDDVGVAAFVEQASGRALDEANLALAQAIYEETEGNPFFVREVLRHLT
                     ETGAVERRDGAWTTRVPIDQLGIPEGVREVVGHRLSRLSHDTNHALRIAAVVGPEFEL
                     GVVQAAGDLGEETLLSALEEAAAARL"
BASE COUNT          258 a          618 c          555 g          226 t
ORIGIN      
        1 agccgggcag cggccgcctc ctccagggcg ctgaggagcg tttcttcgcc gagatcgccg
       61 gcggcctgga ccacgccgag ttcgaactcg ggcccgacga cggcggcgat ccgcagggcg
      121 tggttggtgt cgtgcgacag gcgagagagc cggtggccga ccacttcccg gaccccttcg
      181 gggataccga gctggtcgat tgggacccgg gtggtccagg cgccgtctcg ccgttcgacg
      241 gcccccgtct cggtcaggtg gcggagcact tcccggacga agaacgggtt tccctctgtc
      301 tcctcgtaga tggcctgggc cagggcgagg tttgcctcgt cgagtgcccg gcctgacgcc
      361 tgctcaacga aggccgccac gcccacatcg tcgaggccgg tcagcgacag ccgctcgaca
      421 ccgccttggc ggcgcaggtc gggaacgacc tccagcagag gatgttcgtg ggtcagttcg
      481 ctgtctcgat agatgccgag gacgaggacc cgcccaccac cggcccgcac gacgtggcgc
      541 agaagcatca gcgtcggctt cgctgcccac tggaggtcgt cgagtacgag caggactggt
      601 tcgtcgaccg aggcggcggc cagccaggag gccacaccgt cgaaaaggcg atagcgctcg
      661 gtctcggggt cagaccgcag cggggccggg aggcccggga cccgctcgct cagctcgggc
      721 accaggcggg ccagctcccc gccgtaacga cccagccggc ccggcagcgc gggggcatgg
      781 tccacgaagt ggcgcagggc ctccacgaac ggctggtacg ggacgcccag gtcctcgtcg
      841 cagcgcccgg ccagcaccgc ggccccttca tcgtgggcct tccgtgcgag ttcggcggcc
      901 aggcgggtct tgcccacccc cggctcccca gccaggaggg cgacccgcag ctcgccggcc
      961 atcgcttcct tccacagctg cccgagccgt tcgagctcgc cgtcccggcc gacgaagatg
     1021 cggccgatgt ccgtcaggaa ggtcgggagc gggaccgagg actcaggcag cggctcccag
     1081 ccgacctgga ccgccgggat cggctcgggt agccccttca gctccaggct gccgatcggg
     1141 gtgaatttct cttcgctgga gcgggccagc cagcgaacca gttcggaggc gagaatctgc
     1201 ccgcccgccg ccgcaccaca gagacgagcc gcctcgatga ccggggtgcc aaagcaatcg
     1261 tcggcctcga agaccacgtc ccccacgctg ataccgatcc gtacctccaa aaccgcggcg
     1321 cccgagcggt tgtgccgccc gatcgcctgc tggatcgcca ccgccgcccc caccgcatcc
     1381 gacgccccca cgaacgtggc catgacccca tccccgaggt tcttgacgac ctgtccccgg
     1441 ctcgccttga ccgcgcccgc caccaacgcg tcatgctggt ggcgcaacac gtcggcggcg
     1501 ttctcgccca accgcgaacg aagttccgtc gaccccacca ggtccgtgaa gagcacgatg
     1561 gcggttgacc gccccgcctc gtccccactg ccctgcgcca cggggcgatc gtagaggcgg
     1621 caccccgact acgccatcag acgtactggc ggaggag
//
LOCUS       DASWGG010000349         5253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2880628, whole genome shotgun sequence.
ACCESSION   DASWGG010000349 DASWGG010000000
VERSION     DASWGG010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2880628"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..858)
                     /locus_tag="VF954_06335"
     CDS             complement(190..858)
                     /locus_tag="VF954_06335"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX9530735.1"
                     /translation="MTRPGPVALVGGGEWHDACTFDAGLLEASGQREVLVLPTAAAYE
                     HPERAVEQATRWFAGLGGRVRALMVLARPDAEDEANAEAIRSARFLYLGGGSPLHLRS
                     VLKDSPCWQAVLEAWQAGAVVAGSSAGAMVITDPMVDPRGGAFTLGLGLIEQLAIVPH
                     FDSWSPDKAHRTVSLAAAGLPVVGIDEQTALIRDPAGAWRVEGAGRVTVHQDGAVAGL
                     DALP"
     gene            906..1763
                     /locus_tag="VF954_06340"
     CDS             906..1763
                     /locus_tag="VF954_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX9530736.1"
                     /translation="MSVKRVGIVGSGIMAAGIAECAARNGFEVVVRSRQQATADSMIA
                     GLEKSLAKQVERGKLGDPDRTAALSRVSATSDLGELANCDLVVESIVEDLASKKHLFT
                     ELDRICDEGAILATNTSTLPVVELAMATGRPEKVCGIHFFNPAPVMALVEIVRPLTAS
                     PETIEAARQFALSCGKDAVEVKDQAGFIVNALLFPYLNNAVRLLESGAASREDIDSAM
                     KGGCGFPMGPFALLDLVGLDTSLSILDALYEEFRDPNYAAVPLLRRMVSAELLGRKSG
                     LGFYDYKKK"
     gene            1808..3736
                     /locus_tag="VF954_06345"
     CDS             1808..3736
                     /locus_tag="VF954_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HEX9530737.1"
                     /translation="MIRTYSGHSTARASNELYRTNLARGQTGLSIAFDLPTQTGYDPD
                     AAEAAGEVGKVGVPVAHLGHMRELLEGIPVAETNTSMTINATAAWLLGLYIANAEAQG
                     TDVSRLEGTTQNDIIKEYLSRGTYIFPPGPSLRLIVDMIAYTVAHAPRWNPINVCSYH
                     LQEAGATPVQELAYALATAIGVLDAVRQSGQVAEEAFPHVVGRISFFVNSGIRFVEEA
                     CKMRAFGRMWDQICEERYGVADPKLRRFRYGVQVNSLGLTEAQPENNAQRIVLEMLGV
                     TLSRDARARAMQLPAWNEALGLPRPWDQQWALRMQQVLALESDLLEHDDILAGSHVVE
                     ARTRELIEAAGAELADVLARGGAFGAIDELKSRLVRSQAERTARIEAGEQPVVGVNCF
                     TETAPSPLGGEKSFLTVDPALEAETIAEVVQWRKERDQRSVDGALDELRRVAATAENL
                     MPATIALAHAGGTTGEWADALRQVFGEYRAPTGVAGGAGHHSRSLQALAARSRQMPGG
                     PPRLLVAKPGLDGHSNGAEQIAVAARDAGFEVVYQGIRLTPAQIAAAARDEDVDVVGI
                     SILSGSHLELVPEILRLLRDEGIDVPVVVGGIIPEADQPRLRDAGVAAIYTPKDYQFR
                     PIMDDLARLAGEHRAAAR"
     gene            3803..4531
                     /locus_tag="VF954_06350"
     CDS             3803..4531
                     /locus_tag="VF954_06350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9530738.1"
                     /translation="MDRRVVPGLVAGLAACALALGAFVLRVPFLSVLAGGGALAAVGA
                     CLQLLKAVRAAEEEASGAIALTTVRDMQLAAEHNARRVVDADTGLPDGRFFDLAVDSR
                     VAAARRHLWPMTVVLLEIGLAPDVHSERAQTDALAGFAALMRQTLREADIACRTGPRS
                     FGLLLEDTSEEGGVWTAERLQAALSRDVSRICRLTAGVAAYPSHGLSGPHILLQARQA
                     LHRASAVAPGRGLGRVEVASADLG"
     gene            complement(4863..>5253)
                     /locus_tag="VF954_06355"
     CDS             complement(4863..>5253)
                     /locus_tag="VF954_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530739.1"
                     /translation="HHHPWSPESSTRRSTYFGINPDDSERLVEVVARQAHILGYFAGH
                     THRNRVRHFASTGRVPWAEVACVKDFPGTWAEYRVFEGGVLQVHHRISSPDALAWSER
                     TRVLYAGLYAEYAFGRVEDRCFPILPR"
BASE COUNT          775 a         1882 c         1873 g          723 t
ORIGIN      
        1 tcggcaccaa gggcgccggg accccgaccg agcgggtcgt catcgagtcg gtcaccatca
       61 ccgaggaggc ctagcggcgg cctgcggggc ctgggcattc ttcacgcaaa aagctcgcta
      121 tagcgacaca aattgcttga agaatgccga agcggtgcga tcgggccggc ggcgccgctg
      181 ctacgggtgc tacggcaggg cgtcgaggcc ggccacggcg ccgtcctggt gcaccgtcac
      241 ccgaccggcg ccttccacgc gccacgcccc ggccgggtcc cggatgagcg cggtctgctc
      301 gtcgatgccc acgaccggga gaccggcggc cgccagcgag accgtgcggt gggccttgtc
      361 gggcgaccag ctgtcgaaat gggggacgat ggccagctgc tcgatgagcc ccaggcccag
      421 ggtgaaggcc ccgccccggg ggtcgaccat gggatcggtg atcaccatgg cgccggccga
      481 cgagccggcc acgaccgccc cggcctgcca cgcctcgagg accgcctgcc agcacggtga
      541 gtccttgagc accgagcgca ggtgcagggg cgagcctccc cccaggtaga ggaagcgtgc
      601 cgaccggatg gcttcggcgt tggcctcgtc ctcggcgtcg ggccgggcca gcaccatcag
      661 cgcccgcacg cggccgccga ggccggcgaa ccagcgggtg gcctgttcga cggcccgttc
      721 gggatgctcg taggcggcgg ccgtcggcag gaccagcacc tcacgttggc cgctcgcctc
      781 cagcaggccg gcgtcgaagg tgcaggcgtc gtgccactca cccccgccca ccagggccac
      841 cgggccggga cgggtcacag gtccacccta acaaacggga gctaccgctc ggtaacatgc
      901 agctcatgtc agtcaagcgg gtcgggatcg tcgggtctgg gatcatggcc gcgggcatcg
      961 cggagtgcgc ggcccgcaac ggcttcgagg tggtggttcg cagccgccag caggcgacgg
     1021 cggactccat gatcgccggg ctcgagaagt cactggccaa gcaggtggag cggggcaagc
     1081 tcggggatcc cgaccgcacc gcggccctca gccgggtgtc ggccacctcc gacctgggcg
     1141 agctggccaa ctgcgacctc gtggtcgagt ccatcgtgga ggacctggcc tccaagaagc
     1201 acctgttcac cgagctggac cggatctgcg acgagggcgc catcctggcc accaacacct
     1261 ccacgctccc cgtcgtggag ctggccatgg ccacgggccg gcccgagaag gtgtgcggca
     1321 tccacttctt caacccggcc ccggtcatgg ccctggtcga gatcgtccgg cccctcacgg
     1381 ccagccccga gaccatcgag gcggccaggc agttcgccct gtcgtgcggc aaggacgccg
     1441 tcgaggtcaa ggaccaggcc ggcttcatcg tgaacgccct gctcttcccg tacctcaaca
     1501 acgccgtgcg gctgctggag tcgggcgccg cgtccaggga ggacatcgat tcggccatga
     1561 agggcggctg cggcttcccg atgggccctt tcgccctcct cgacctggtc ggcctggaca
     1621 ccagcctgtc catcctggac gcgctgtacg aggagttccg cgaccccaac tacgccgccg
     1681 tgcccctcct gcgcaggatg gtcagcgcgg agctgctcgg ccggaagtcg ggactgggct
     1741 tctacgacta caagaagaag taggcctcag gccgggggaa gattgagcca tgcctgaccg
     1801 cccttggatg atacggacct actccgggca ttcgacggct cgggcgtcca acgagctcta
     1861 ccggaccaac ctggccaggg gccagaccgg gctgtcgatc gccttcgacc tgccgaccca
     1921 gaccggctac gacccggacg cggccgaggc ggccggtgag gtgggcaagg tcggcgtgcc
     1981 cgtcgcccac ctgggccaca tgcgggagct gctcgagggc atcccggtcg ccgagacgaa
     2041 cacctccatg accatcaacg ccaccgcggc gtggctgctg gggctgtaca tcgccaacgc
     2101 cgaggcccag ggcaccgacg tgtcccggct cgaaggcacg acccagaacg acatcatcaa
     2161 ggagtacctg tcccgcggga cctacatctt cccgccggga ccgagcctgc ggctgatcgt
     2221 ggacatgatc gcctacaccg tcgcccacgc gccccggtgg aaccctataa acgtgtgcag
     2281 ctaccacctc caagaggccg gggccacgcc cgtgcaggag ctggcgtacg cgctggccac
     2341 ggccatcggc gtgctggatg cggtccgcca gtcgggacag gtcgccgagg aggccttccc
     2401 ccacgtggtg ggccgcatct cgttcttcgt caactcgggc atccggttcg tcgaggaagc
     2461 ctgcaagatg cgggccttcg ggcgcatgtg ggaccagatc tgcgaggagc gctacggggt
     2521 ggccgacccc aagttgcggc gcttccgcta cggcgtgcag gtgaactcgc tcgggctcac
     2581 cgaggcccag ccggagaaca acgcccagcg catcgtgctg gagatgctcg gggtcaccct
     2641 gtcgcgggat gcccgggccc gggccatgca gctgccggcc tggaacgagg cgctcggcct
     2701 gccccgcccc tgggaccagc agtgggccct ccggatgcag caggtcctgg ccctggagag
     2761 cgacctgctg gagcacgacg acatcctcgc cggctcgcac gtggtcgaag cccgcacccg
     2821 ggagctgatc gaggcggccg gggccgagct ggcagacgtg ctggctcggg gcggcgcgtt
     2881 cggcgccatc gacgagctca agtcccgcct ggtacgcagc caggccgagc gcacggcccg
     2941 gatcgaggcc ggcgagcaac ccgtcgtggg cgtcaactgc ttcacggaga cggccccgtc
     3001 gccgctgggc ggcgagaaga gcttcctcac ggtcgacccc gccctcgagg ccgagaccat
     3061 cgccgaggtc gtccagtggc gcaaggagcg ggaccagcgc tccgtcgacg gggccctcga
     3121 cgagctgcgc cgggtggccg ccacggcgga gaacctcatg ccggccacca tcgccctggc
     3181 ccatgccggc gggaccacgg gcgagtgggc cgacgccctg cgccaggtgt tcggcgagta
     3241 ccgggcgccc accggggtgg cgggcggggc cgggcaccac tcgcgctccc ttcaggcgct
     3301 ggccgcccgc tcgaggcaga tgcccggtgg cccgccccgg ctgctggtgg ccaagccggg
     3361 cctggacggc cactccaacg gggccgagca gatcgcggtg gcggcgcgag acgccggctt
     3421 cgaggtggtg taccagggca tccggctcac gccggcccag atcgccgccg cggcacggga
     3481 cgaggacgtg gacgtcgtcg ggatctccat cctgtccggc agccacctcg agctggtgcc
     3541 cgagatcctc aggctcctgc gcgacgaggg catcgacgtg cccgtggtcg tcggcgggat
     3601 catcccggag gccgaccagc cccgcctccg ggatgccggc gtcgccgcca tctacacgcc
     3661 caaggactac cagttccggc cgatcatgga cgatctggcc cggctggccg gcgagcaccg
     3721 ggcggcggcg cggtgagccg atcttcgatt tgatcgactt ttgggcttga tcatggggcg
     3781 caggtagccg atggtgtagg caatggaccg acgcgtcgtg ccggggttgg tcgcggggct
     3841 ggctgcgtgt gcgctcgcgc tgggtgcctt cgtgctgcgt gtccccttcc tgagcgtgct
     3901 cgccggcggc ggcgccctcg ccgcagtcgg cgcgtgcctc cagctcctca aggccgtgcg
     3961 ggccgccgag gaggaggcgt cgggcgccat cgccctcacg accgtccggg acatgcagct
     4021 ggccgcagag cacaacgccc gccgcgtggt cgacgccgat acgggcctcc cggacggccg
     4081 gttcttcgac ctggccgtcg acagccgggt ggctgccgcc aggcgccacc tgtggccgat
     4141 gaccgtggtc ctgctggaga tcggcctggc gcccgacgtc cacagcgagc gggcccagac
     4201 cgacgccctg gcggggttcg cggccctgat gcgccagacg ctgcgggagg ccgacatcgc
     4261 ctgccggacg gggccgaggt cgttcggcct cctgctggag gacaccagcg aggagggtgg
     4321 ggtgtggacg gcggagcgcc tccaggccgc gctgtcccgc gacgtctccc gcatctgccg
     4381 gctgacggcc ggggtggccg cctacccgtc ccacggcctc tccggtcccc acatcctgct
     4441 ccaggcccgc caggccttgc accgggcctc ggccgtcgcg cccgggcgcg gcctgggccg
     4501 ggtcgaggtg gcttcggccg acctgggctg aggaccgggc tgaggacccg cccgcccggc
     4561 cagtgctggg gggaaacggg tgcccacgga gggtgacagg tgtcccatgt tcagccggag
     4621 gtcgggtaaa tccccagcct cgcggggttt tccccagggt tttcccccgt ttgtgcggtg
     4681 actagcacct ccgagccatc cccggcaatg ccgaacgggc cgaccaactg cccgtaacga
     4741 ctagtccgac cattgaccgg agacctgagc acgagccccg gcgcgccctt tgtcaacgca
     4801 cggctcgcct acgcgagcca cgttatcccc aacgggtcag ctctccgccg gtctcggcgc
     4861 gatcaccggg ggaggatcgg gaagcaccgg tcctccacgc ggccgaacgc gtactcggcg
     4921 tatagaccgg cgtacagcac ccgggtgcgc tcagaccacg ccagcgcgtc cgggctggag
     4981 atgcggtggt ggacctggag cacgccgccc tcgaacaccc ggtactccgc ccacgtgccg
     5041 gggaagtcct tgacgcacgc cacctcggcc cacggcaccc ggccggtgga ggcgaaatgg
     5101 cgcacgcggt tgcgatgggt gtggccggcg aaatagccga ggatgtgcgc ctgccgggcc
     5161 accacctcca ccaggcgctc ggagtcgtcg ggattgatcc cgaaatacgt cgagcggcgc
     5221 gtgctggact cggggctcca gggatggtgg tgg
//
LOCUS       DASWGG010000350         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_469856, whole genome shotgun sequence.
ACCESSION   DASWGG010000350 DASWGG010000000
VERSION     DASWGG010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469856"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..267
                     /locus_tag="VF954_06360"
     CDS             67..267
                     /locus_tag="VF954_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX9530740.1"
                     /translation="MKVLLRNPRREIDVAGQVRVHELLRRLDVNREAVLVICNHTLVT
                     GDALLHEADLVEVRPVISGGSA"
     gene            264..1178
                     /locus_tag="VF954_06365"
     CDS             264..1178
                     /locus_tag="VF954_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)-lysidine synthetase"
                     /protein_id="HEX9530741.1"
                     /translation="MRCRRCREPAVIEIRRHNAGFCRDCFLHHCREQVRRAIEDHEML
                     RPEDRVLVAVSGGKDSLALWDILLDLGYAADGLYLGLGIGDYSDASGRCARAYAAGRT
                     ARLVEVDIPADYGFGIPEGSAAARRVPCSACGLSKRHLFNAAARDGGYDVMATGHNLD
                     DEAAVLLGNVLRWSTPYLGRQLPVLPATPGFARKVKPLIRLAEREMAAYCVLTGIDYI
                     VEECPMAEGNRHLGYKEALNAVEDRSPGTKSAFYFGFLERAAPLFAPAASAEQAGLRD
                     CASCGAPTTGETCAFCKLLEKATRPEGG"
     gene            1175..2008
                     /locus_tag="VF954_06370"
     CDS             1175..2008
                     /locus_tag="VF954_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEX9530742.1"
                     /translation="MSRPFEVGDRVLLIDSKSRRYLVTLAPEGSFHSHAGYVTHGVLL
                     GRPEGTTVRSTGGARYLAVRPTLSDFILKMPRGAQVIYPKDLGPLLLIADIFPGARVL
                     EAGVGSGALSMTMLRAGAEVVGYELREDFAARARANVASFLGPEASARFRVEGRDVYA
                     GIDEADLDRVVLDLPEPWRAVKHASAALHPGGILVSYLPSVSQVAALREAIEDSPFGL
                     AHTVEVLERGWHVEGQSVRPDHRMVGHTGFLTSARLLGGPTGGPAGGAPEPGEDRPGP
                     G"
     gene            2005..>2216
                     /locus_tag="VF954_06375"
     CDS             2005..>2216
                     /locus_tag="VF954_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530743.1"
                     /translation="MNLLDIIVVVAAVSAVVGGWRLGFLARAASWIGLGLGLYVAARL
                     LPAAIRTFDGSTPTTRLLVAAVVLIGG"
BASE COUNT          265 a          818 c          812 g          321 t
ORIGIN      
        1 cgcccaggag gccgacgccg tgctggtcga tcgctgacgc ccgtcggttt ccgcgacaat
       61 gcccctgtga aggtgttgct ccgcaacccg cgtcgggaga tcgacgtggc cggccaggtg
      121 cgggtccacg agctgttgcg ccgcctcgac gtcaaccgcg aggcggtgct ggtcatctgc
      181 aaccacaccc tggtgacggg cgacgccctg ttgcacgagg ccgacctggt cgaggtcagg
      241 ccggtcatct ccggcggctc ggcgtgaggt gccgccgctg ccgcgagccg gccgtcatcg
      301 agatccggcg ccacaatgcg ggcttctgcc gggactgctt cctgcaccac tgccgggagc
      361 aggtccgcag ggccatcgag gatcacgaga tgctccggcc cgaggaccgc gtcctggtgg
      421 ccgtgtcggg aggcaaggac tcgctggccc tctgggacat cctgctggac ctgggctacg
      481 ccgccgacgg cctgtacctc gggctgggca tcggcgacta cagcgacgcc tcgggccgct
      541 gcgcccgggc gtacgcggcc gggcgcacgg cccgcctcgt cgaggtggac atcccggccg
      601 actacggctt cggcatcccg gagggctcgg ccgcggcccg gcgggtcccg tgctcggcct
      661 gcgggctctc caagcgccac ctgttcaacg ccgcggcccg cgacggcggc tacgacgtga
      721 tggcgaccgg ccacaacctg gacgacgagg cggccgtgct gctgggcaac gtgctgcgat
      781 ggtcgacgcc ctatctcggt cgccagctgc cggtgctgcc cgccaccccc gggttcgccc
      841 gcaaggtcaa gcccctcatc cggctggccg agcgggagat ggccgcctac tgcgtgctca
      901 cgggcatcga ctacatcgtg gaggagtgcc cgatggcgga gggcaaccgg cacctcggct
      961 acaaggaggc cctcaacgcc gtggaggacc ggtcaccggg caccaagagc gccttctact
     1021 tcggcttcct ggagcgggcc gcgcccctgt tcgcccccgc ggcctccgcc gagcaggccg
     1081 gcctgcggga ctgcgcctcc tgcggggcgc cgaccaccgg ggagacctgc gccttctgca
     1141 agctgctcga gaaggccacc aggccggagg gcgggtgagc cgcccgttcg aggtcggcga
     1201 ccgggtgctg ctgatcgact cgaagagccg ccggtacctc gttaccctgg cccccgaggg
     1261 gtccttccac agccacgccg gctacgtgac ccacggcgtg ctgctgggcc gcccggaggg
     1321 caccaccgtc cgctcgacgg gcggggcccg ttacctggcg gtgcggccga ccttgtcgga
     1381 cttcatcctg aagatgcccc gcggtgcgca ggtcatctac cccaaggacc tgggccctct
     1441 cctgctcatc gccgacatct tcccgggcgc ccgcgtcctg gaggccggcg tgggatccgg
     1501 ggcgctgtcc atgacgatgc tgcgagccgg ggcggaggtg gtcggctacg agctgcggga
     1561 ggacttcgcg gctcgggccc gggccaacgt ggcgtcgttc ctcgggcccg aggcctcggc
     1621 ccggttccgg gtcgaggggc gtgacgtgta tgccgggatc gacgaggcgg acctcgaccg
     1681 cgtggtgctg gacctgcccg aaccctggcg ggcggtcaag cacgccagcg cggcgctgca
     1741 ccccggcggg atcctggtgt cgtacctgcc gagcgtgtcc caggtggcgg cgctgcggga
     1801 ggcgatcgag gacagcccct tcggcctggc ccacaccgtc gaggtcctcg agcggggctg
     1861 gcacgtcgag gggcagtccg tccggcccga ccaccgcatg gtcggccaca ccggcttcct
     1921 cacgtcggcc cgcctgctgg gcgggccgac cggcggtccc gccggcggcg cacccgagcc
     1981 gggcgaggac cgaccggggc ccgggtgaac ctgctcgaca tcatcgtcgt ggtggcagcc
     2041 gtgtcggccg tggtgggcgg ctggcggctc ggcttcctcg cccgggcggc gtcgtggatc
     2101 ggcctcgggc tcggcctgta cgtcgcggcc cgcctgctcc cggccgccat ccgcaccttc
     2161 gacggctcga cgccgaccac ccgcctgctg gtcgcggccg tcgtgctcat cggcgg
//
LOCUS       DASWGG010000351         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_3212364, whole genome shotgun sequence.
ACCESSION   DASWGG010000351 DASWGG010000000
VERSION     DASWGG010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3212364"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1964
                     /locus_tag="VF954_06380"
     CDS             <1..1964
                     /locus_tag="VF954_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9530744.1"
                     /translation="NLLELEERGAGDELIAALFRDAHTLKGSAAVVGLLGFSSVAHAM
                     ESLLDDLRQGRRPPEPDLVDALLVAVDGLRRLLPTFLGGGEAAAETDALARQLAQLPP
                     PPPPEPPASSGPADPASIGVTVSAAPVIRVPVGRLEAMTGLVGESAAAHLRLGRAIEH
                     RLGPGAVELPEVHDLSRLLNTLQETTMRARMVPIATVVEPLRRTVREVARRQGKDVRW
                     EVSGESTELDRNVLDRLGEALLHLVRNAVDHGVEAPDVRAATSKPATATVRLHAMQVG
                     PEVVVTISDDGRGIDLDELRGQAGVAPELDEGDTLQLLFRAGFSTAATVTDVSGRGVG
                     LDVVKTAIDALRGRVEVRTLPGQGAEFRLVVPITLAVLRCLLIEAGGSSYAVPMHSVA
                     TVLGPGEAATSIVGRPAVAVAGGQIAVSDLGATLGGPPEPPARGPVVVVTSADRRHAF
                     RVDRLLRQREVVVKNLSPLLPRIDVLAGASVEPDGSVLYVLDASGLIERARRGGAAGP
                     PRGSADLRGAAASETSSAAVLVVDDAPSVLAMQHAMLSRAGYRVLTAADGQEALAALR
                     STAIDLVITDLEMPRMDGIELVRAIRSDPGLTETGVIVLTAHGSEDHRRAGMEAGADA
                     YVVKSPFDQAAFLALVARLVGPPIGGGRP"
     gene            1961..>2462
                     /locus_tag="VF954_06385"
     CDS             1961..>2462
                     /locus_tag="VF954_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9530745.1"
                     /translation="MTRGSGAKPAPRRVVVVEDSGVQRANLRRVIEADGDLAVVAEAG
                     TAAEAVLAVRQARPHVATIDLDIPGGGLEAIRSIMATRPTPLLVVSAAVGGLRAASVV
                     EALASGAADALPKPVRWSPGAEDDVRARIRALTGIAIRSVPPTVSGCADVVVAVAAST
                     GGPAALA"
BASE COUNT          284 a          933 c          914 g          331 t
ORIGIN      
        1 agaaccttct ggagttggag gagcgcggtg ccggcgacga gctgatcgcc gcgctcttcc
       61 gggacgccca taccttgaag ggctcggccg ccgtcgtcgg cctcctcggg ttcagctctg
      121 tggcccacgc catggagagc ctgctggatg acctgcgcca gggccggcgg ccgcccgagc
      181 ccgacctggt ggacgccctg ctggtcgcgg tcgacggcct gcgccggctt ctgcccacct
      241 tcctcggagg cggggaggcg gccgccgaga cggatgccct cgcccgccag ctcgcccaac
      301 tgcctccgcc gccgcctccg gagccccctg cgagctctgg gcccgccgac ccggcgtcga
      361 tcggggtgac ggtgtcggcc gcgccggtca tccgggttcc ggtcgggcgc ctcgaggcca
      421 tgaccggcct cgtgggcgag tcggccgccg cccacctccg cctgggccgg gcgatcgagc
      481 accgcctggg tcccggcgcc gtcgagcttc ccgaggtgca cgacctctcg cgcctgctca
      541 acaccctcca ggagaccacc atgcgggccc gcatggtgcc gatcgccacg gtcgtcgaac
      601 ccttgcgaag aacagttcgg gaggtggccc ggcgccaggg gaaggacgtg cggtgggagg
      661 tgtccgggga gtcgaccgag ctggaccgca acgtcctcga ccgcttgggg gaggcactgc
      721 tgcacctggt ccgcaacgcc gtcgaccacg gcgtcgaggc cccggacgtc cgcgccgcga
      781 cgagcaagcc ggcgacggcg acggtccggc tccacgccat gcaggtcgga ccggaggtgg
      841 tcgtcaccat cagcgacgac gggcggggca tcgatctcga cgagctgcgc gggcaggcgg
      901 gcgtagcgcc ggagctggac gagggcgaca ccctccagct cctgttccgg gccgggttct
      961 ccaccgccgc cacggtcacc gacgtgtccg gacgcggcgt ggggctggac gtggtgaaga
     1021 ccgccatcga cgcgctgcgg ggccgcgtcg aggtccggac cctgcccggc cagggcgcgg
     1081 agttccgcct cgtcgtaccg atcacgctgg ccgtgctgcg ctgcctgctg atcgaggcgg
     1141 gcggcagctc ctacgccgtg ccgatgcact cggtcgccac cgtgctcggc cctggcgagg
     1201 ccgccacgag catcgtcggc cggccggcgg tcgcggtcgc gggaggccag atcgcggtca
     1261 gcgacctggg ggcgaccctc ggcgggccgc ccgagccgcc tgcccggggg cccgtcgtgg
     1321 tcgtgaccag cgccgaccgc cggcacgcct tccgcgtcga ccgcctgctg cgccagcggg
     1381 aggtcgtcgt caagaacctc agccccctcc tcccccgcat cgacgtcctg gcgggcgcga
     1441 gcgtggagcc ggacgggtcc gtgctctacg tgctcgacgc gtccggcctg atcgagcggg
     1501 cccggcgcgg cggcgcggcc ggcccgccca gagggtccgc cgatctccgc ggggccgccg
     1561 cctcggagac gagctcggcg gcggtgctcg tggtggacga cgccccgtcg gtcttggcca
     1621 tgcagcatgc catgctgagc cgggccgggt accgcgtcct caccgccgcc gacggccagg
     1681 aggccctggc cgccctcagg tcgacggcga tcgatttggt gatcaccgac ctcgagatgc
     1741 cgcgcatgga cgggatcgag ctggtgcggg ccatccggtc cgaccccggt ctgaccgaga
     1801 ccggcgtgat cgtcctgacg gctcacggct cagaggacca ccggcgagcc ggcatggagg
     1861 ccggagccga cgcctacgtg gtgaagagcc ccttcgacca agccgccttc ctcgccttgg
     1921 tggccaggct ggtcggaccg ccgatcgggg gtggccgccc gtgacgcgcg gtagcggggc
     1981 caagccggcg cctcggcggg tggtggtcgt cgaggactcc ggcgtgcagc gggcgaacct
     2041 ccggcgggtc atcgaggcgg acggcgatct cgcggtcgtg gccgaggcag gcaccgcggc
     2101 cgaagccgtc ctggccgtcc ggcaggctcg gccccacgtg gcgaccatcg acctcgacat
     2161 ccccggcggc ggcctggagg ccatccgctc gatcatggcc acccgtccca cgcctctgct
     2221 cgtcgtgtcg gccgccgtcg gggggctgcg cgccgcgtcc gtcgtcgagg cgctcgcttc
     2281 aggcgccgcc gacgccctcc cgaagcccgt ccgttggtcg cctggtgccg aggacgacgt
     2341 gcgcgcccgc atccgggccc tcacgggcat cgccatccgc tccgtcccgc cgacggtcag
     2401 cggttgcgcc gacgttgtcg tggccgtcgc cgcgtcgacc ggcggaccgg cggctctcgc
     2461 cc
//
LOCUS       DASWGG010000352         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2084747, whole genome shotgun sequence.
ACCESSION   DASWGG010000352 DASWGG010000000
VERSION     DASWGG010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084747"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VF954_06390"
     CDS             complement(<1..881)
                     /locus_tag="VF954_06390"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX9530746.1"
                     /translation="MPEAVIVSTARTPIGRAQKGSLRDNRIDDLGGFIVKTLLEKVPA
                     VEPASVDDVIIGAANHSGEQGMNAARNIGLLAGLPDTVPGTTVNRFCASSLQSIRMAF
                     HAIKAGEGDTYVAGGVESLSRTGGRGMNRDDLNPRFMDASRPDFINQVYIPMGLTAEN
                     VAEKFGVSRERMDELALISQNRAVAAQENGFFEREITPVTTPDGTVVAKDDGPRPGTT
                     LEQLAQLQPAFKRDGTVTAGNSCPLNDGAAAVLVMSADRAADLGLRPLARVVASAVSG
                     LAPEIMGVGPIEAVTRVL"
     gene            complement(927..2084)
                     /locus_tag="VF954_06395"
     CDS             complement(927..2084)
                     /locus_tag="VF954_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HEX9530747.1"
                     /translation="MPPAKLHWAHLYDEVVTSGLCTGCAGCIVACPYDVLGYDDSNGV
                     YKPFQLDPAAAPANCIHGEKGCTLCTRACPRFRDWEPELDNFLFGRPRETTEVEGISK
                     DIVLARAVDQEVHDRGQDGGLVSAILLWAIEHDYIDAALVSYLEGDGTSWKAVPGVAR
                     TREEILAGAGSRYTYSANTMAYASAIESGAEKIALVGMSCQSSVPPALSSRKAGKAAR
                     RFALNIGLLCSKTFDDAIFEELFEAKYGLHKQDMVKMNIKGVFQIWMRDGSYHEVDLK
                     ECHAWTREGCKTCPDFAAEHADISTGGIGAFNDWTLTIVRTDTGREIMDGMLRDGVIE
                     VRPGDDDPGAITLLRRLSRVSRRRWPETAVAEPRRVPPPKAKPATPQPAQV"
     gene            2181..2966
                     /locus_tag="VF954_06400"
     CDS             2181..2966
                     /locus_tag="VF954_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX9530748.1"
                     /translation="MPELPEVEAYRRIGSRALGRPIVGVHAPDAWFLKGGLSAAGLSE
                     AMVGRALVAVRRVGKVLLFDTSGPGPVLGLRFGMTGRLLVDGAAGVDRLLHSSRREEE
                     RWDRCRLRFDDGGDLRIRDPRRLGGVSLDPREDLLGPDAAAITPAQLRAALAGSRVAL
                     KARLLDQRRVAGVGNLMADETLWRSALSPLRPAGGLTTAEHRRLHRHLRSTVADLIER
                     GGSHLGDLIPHRVPGGRCPRDGAELRSDTVGGRATWWCPRHQR"
     gene            complement(3003..3224)
                     /locus_tag="VF954_06405"
     CDS             complement(3003..3224)
                     /locus_tag="VF954_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530749.1"
                     /translation="MSDDHVPQDGDPVRRAAGRAVDVPDADALEQASPAGEEEEVDTW
                     RITAERSIDVPEADALEQAEEVTDWDERD"
     gene            complement(3280..>3528)
                     /locus_tag="VF954_06410"
     CDS             complement(3280..>3528)
                     /locus_tag="VF954_06410"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HEX9530750.1"
                     /translation="AFAAEKSRHPELRDEFERVFVAERRARVRQLIARGVASGQLPAS
                     TDVELLAEAGFAILWHRLSLQRPLSPDLPERIVRQFLA"
BASE COUNT          458 a         1228 c         1294 g          548 t
ORIGIN      
        1 agcaccctgg tcacggcctc gatcgggccc acgcccatga tctccggcgc cagtcccgac
       61 accgcgctgg cgacgacgcg ggcgagcggc ctgagcccga ggtcggcggc gcggtccgcg
      121 gacatcacga gcacggcggc ggcaccgtcg ttgagggggc aggagttgcc ggccgtgacc
      181 gtcccgtcgc gcttgaaggc gggctggagc tgggccagct gctcgagcgt cgtgcccggc
      241 cgcggcccgt cgtctttcgc caccacggtg ccgtcgggcg tcgttacggg cgtgatctcc
      301 cgctcgaaga agccgttctc ctgggcggcc acggcccggt tctgggagat caacgcgagc
      361 tcgtccatgc gctcccgtga cacgccgaac ttctcggcca cgttctccgc cgtcaacccc
      421 atcgggatgt acacctggtt gatgaagtcg gggcgggatg cgtccatgaa ccggggattg
      481 aggtcgtcgc ggttcatgcc ccgcccgccg gtgcggctga ggctctccac cccgcccgcc
      541 acgtaggtgt cgccctcgcc cgccttgatg gcgtggaacg ccatgcggat ggactgcagc
      601 gaggaagcgc agaagcggtt caccgtggtg cccggcaccg tgtcgggcag tccggcgagc
      661 aggccgatgt tgcgggccgc gttcatgccc tgctccccgg agtggttggc cgcgccgatg
      721 atcacgtcgt cgacggatgc gggctcgacg gcgggcacct tctcgaggag cgtcttgacg
      781 atgaaaccgc ccaggtcgtc gatgcggttg tccctgagcg agcccttctg ggcccggccg
      841 atgggtgtgc gggccgtgct gacgatgact gcttcaggca tggcgtctcc tcggctcggt
      901 gtgccgcggg gctggcgctg gtgagctcag acctgggctg gctgcggggt ggcgggcttg
      961 gccttgggcg gcggtacgcg acgcggctcg gcgacggccg tctccggcca gcgtctgcgg
     1021 ctcaccctgg acagacgtcg gaggagggtg atggcacctg ggtcgtcgtc gcccggccgg
     1081 acctcgatga cgccgtcgcg cagcatgccg tccatgatct cccggccggt gtcggtccgg
     1141 acgatggtca gggtccagtc gttgaaggcg ccgatgccgc cggtggagat gtcggcatgc
     1201 tccgccgcga agtcggggca ggtcttgcac ccctcgcggg tccaggcgtg gcactccttg
     1261 aggtcgacct cgtggtagct gccgtccctc atccagatct ggaacacgcc cttgatgttc
     1321 atcttcacca tgtcctgctt gtggagcccg tacttggcct cgaaaagctc ctcgaagatg
     1381 gcgtcgtcga aggtcttgga gcagagcagg ccgatgttca gggcgaagcg gcgtgccgcc
     1441 ttgccggcct tgcgcgagga cagagcggga gggaccgacg actggcagct catgccgacc
     1501 agggcgatct tctccgcgcc cgactcgatg gccgacgcgt aggccatggt gttggcggag
     1561 tacgtgtagc gggagccggc gccggcgagg atctcctcgc gggtgcgggc gacgccgggt
     1621 acggccttcc agctggtgcc gtcgccttcg aggtaggaca ccagggcggc gtcgatgtag
     1681 tcgtgctcga tggcccagag caggatggcg gacacgaggc cgccatcctg gccgcgatcg
     1741 tggacctctt gatcgacggc ccgggccagc acgatgtcct tggagatccc ttcgacctcg
     1801 gtggtctccc tgggccggcc gaacaggaag ttgtcgagct ccggctccca gtcgcggaat
     1861 cgggggcagg cccgggtgca cagggtgcac cccttctccc cgtggatgca gttggccgga
     1921 gcggccgcgg ggtcgagctg gaagggcttg tacacgccgt tgctgtcgtc gtagccgagc
     1981 acgtcgtatg ggcaggccac gatgcacccg gcgcagcccg tgcagagccc ggacgtcacg
     2041 acctcgtcgt agaggtgggc ccagtgcagc ttggcgggag gcattcgcgc accttaggcc
     2101 gccgggcgcg gcccggtcac acgcgccgcc gccggtgggc gcggagccac cctttctgcc
     2161 gaccggtacc gtcgggcgcc gtgcccgagc taccggaggt ggaggcctac cgccgcatcg
     2221 gctcccgggc gctcggccga ccgatcgtcg gggtgcacgc gccggacgcg tggttcctga
     2281 aaggggggtt gtccgccgcc ggcttgtcgg aggccatggt ggggcgcgcc ctcgtggcgg
     2341 tccgtcgcgt ggggaaagtc ctgctcttcg acacgtcagg gcccgggccg gtcctggggc
     2401 tgcgcttcgg catgacgggc cggttgctag tggacggtgc ggccggtgtg gaccggctgc
     2461 tccactcgag caggcgtgag gaggagagat gggaccggtg ccggcttcgc ttcgacgacg
     2521 gcggagacct gaggatccgg gacccgcggc gcctgggggg cgtgtcgctc gacccacgcg
     2581 aggacctgct gggcccggac gcggccgcca tcaccccggc ccagctgcgt gccgcgctgg
     2641 ccgggagccg ggtcgcgctc aaggcccggc tcctcgatca acgacgagtg gccggcgtgg
     2701 gcaacctcat ggcggacgag acgttgtggc gatccgcgct ctcgcccctt cgcccggcgg
     2761 gcggcctcac caccgccgag caccggcggc tgcaccggca cctgcggtcc acggtcgccg
     2821 acctgatcga gcggggcggg tcgcacctcg gcgacctgat tccgcaccgg gtcccgggcg
     2881 gacgctgccc ccgcgacggg gcggagctgc ggagcgacac cgtaggcggg cgcgcgacgt
     2941 ggtggtgccc gagacaccag aggtgacgct gtggacccgg gcgagccccg actccgccag
     3001 tgtcagtccc gctcgtccca gtccgtcacc tcctccgctt gctccagtgc gtccgcctcg
     3061 ggcacatcga tggaccgctc ggccgtgatc cgccaggtat cgacctcctc ttcttcgccg
     3121 gcgggactcg cctgctccaa ggcgtcggca tccggcacgt ccaccgcccg ccccgcagcc
     3181 ctacgcaccg gatcgccgtc ttgggggacg tggtcatcgg acatcacata gtcctacccc
     3241 acgcgggccg gcgcgcgacg cccgggcccc ggcacctgcc tacgccagga actgccggac
     3301 gatccgctcc ggcaggtccg gcgacaacgg ccgctggagg ctcagccgat gccagaggat
     3361 ggcgaagccc gcctcggcca gcagctccac gtccgtcgac gcgggaagct gccccgacgc
     3421 caccccgcgg gcgatgagct ggcggacccg ggcccggcgc tctgcaacga agacccgctc
     3481 gaactcgtcc cgcagctccg ggtggcggga cttctcggca gcgaacgc
//
LOCUS       DASWGG010000353         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2884398, whole genome shotgun sequence.
ACCESSION   DASWGG010000353 DASWGG010000000
VERSION     DASWGG010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2884398"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VF954_06415"
     CDS             <1..596
                     /locus_tag="VF954_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9530751.1"
                     /translation="SGKSTLMHCQAGLDAVTSGNVYLGQTDLSSLDDKGLTQLRRDQV
                     GFIFQAYNLLPTLTALENITLPLDLAGRRPDQAWLDQVIDTVGLRPRLTHRPTELSGG
                     QQQRVAVARALATRPRIVFADEPTGNLDSRAGAEVLGFLRQSVDDLGQTIVMVTHDPT
                     AAGYADSAVFLADGHIVDEMEGPTAERVLDRLKTLES"
     gene            625..>1671
                     /locus_tag="VF954_06420"
     CDS             625..>1671
                     /locus_tag="VF954_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9530752.1"
                     /translation="MIKGLLAHKLRLALTALSVVLGVGFVAGTFVLTDTLAHTFDNLF
                     AEVNGKTDAQIRSTQKLSPQDPAESDRGPVPASLLAVIQKTSGVAEAGGYVQGRAAIV
                     GRDGKIVGGQAPNFGASASNLGTLSPFSVKDGRAPKGPDEVAIDAGTAKREHFAIGDR
                     IKIEASVTGTYTVVGIVGFGSADNLAGAKFALWDVPTAQRVLHRLGEFDIISVKAAAG
                     VTRPQLVDRLQPVLPKGLEAITSDVAAAQQADQVKKSLGFLSTFLLAFAAVSLFVGSF
                     IIYNTFTIIVAQRLRELALLRALGASRRQVTRSVITEGVLVGFFASLVGVGFGVFVAM
                     GLKAVFAAVGADLPS"
BASE COUNT          248 a          600 c          570 g          253 t
ORIGIN      
        1 gttcagggaa atcgacgctg atgcactgcc aggcagggct cgacgccgtc accagcggca
       61 acgtctacct gggccagacc gacctgtcgt cgctggacga caaagggctc acccagctgc
      121 ggcgggacca ggtcggcttc atcttccagg cctacaacct gctgccgacg ctcacggccc
      181 tggagaacat cacgctgccg ctcgacctgg ccggacgcag acccgaccag gcctggctgg
      241 accaggtgat cgacaccgtt gggctgcggc cccggctgac ccaccggccg acggagctgt
      301 cgggcgggca gcagcagcgg gtggcggtgg cccgggcgct ggccacccgg ccccggatcg
      361 tgttcgccga cgaacccacc ggcaacctcg attcccgggc cggggccgag gtgctgggct
      421 tcctccgcca atcggtcgac gacctcggcc agaccatcgt gatggtcacc cacgacccga
      481 ccgccgccgg gtacgccgat tccgcagtgt tcctggccga cgggcacatc gtcgacgaga
      541 tggagggccc gacggcggag cgggtgctcg accgcctcaa gacgctggaa tcgtgagggg
      601 gggcggagcc cagtgcgccg ggtgatgatc aagggcctac tggcccacaa gctgcggctg
      661 gcgctgaccg ccctgtcggt ggtgctcggc gtcggcttcg tggccggcac gttcgtgttg
      721 accgacacgt tggcccacac cttcgacaac ctcttcgccg aggtcaacgg caagaccgac
      781 gcccagatcc gctccaccca gaagctctcc ccccaggatc cggccgagtc cgatcggggc
      841 ccggtcccgg cgtcgctgct ggcggtgatc cagaagacga gcggcgtggc cgaagccgga
      901 ggctatgtgc agggccgggc ggcgatcgtc ggccgggacg gcaagatcgt gggtgggcag
      961 gccccgaact tcggtgcctc ggcctccaac ctcgggacgc tctcgccgtt ctcggtcaag
     1021 gacggtcggg cccccaaggg ccccgacgag gtggccatcg acgccggcac cgccaagcgg
     1081 gaacacttcg ccatcgggga ccggatcaag atcgaggcca gcgtcaccgg gacgtacacc
     1141 gtggtcggca tcgtcggctt cggttcggcc gacaacctgg ccggggccaa gttcgcgctg
     1201 tgggacgtgc ccacggccca gcgggtcctc caccgcctgg gcgagttcga catcatctcg
     1261 gtcaaggccg cggcgggcgt gacgaggccc caactcgtgg atcggctcca accggtgctg
     1321 ccgaagggcc tcgaggccat caccagcgac gtggccgccg cccagcaggc cgaccaggtg
     1381 aagaagtcgc tcggcttcct cagcacgttc ctgctggcct tcgccgccgt gtcgctcttc
     1441 gtcgggtcgt tcatcatcta caacaccttc acgatcatcg tggcccagcg gctccgggag
     1501 ctggcgctcc tccgggccct cggtgccagc cggcggcagg tcacccggtc cgttatcaca
     1561 gaaggggtcc tggtcggatt cttcgcctcg ctcgtcggcg tcggcttcgg cgtcttcgtg
     1621 gccatggggc tcaaggccgt cttcgccgcg gtcggcgccg acctcccgtc c
//
LOCUS       DASWGG010000354         3704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1929781, whole genome shotgun sequence.
ACCESSION   DASWGG010000354 DASWGG010000000
VERSION     DASWGG010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929781"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            854..2695
                     /gene="infB"
                     /locus_tag="VF954_06425"
     CDS             854..2695
                     /gene="infB"
                     /locus_tag="VF954_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX9530753.1"
                     /translation="MEELEPTTLTTYTPSNAPVPEGETIIERGSTAQDVGPKLNRSAG
                     DVVRFLLLQGEMVTATQSLSDDMIELFAAELGATVHLVDPGEEKEAALQAKYFDDDDE
                     GDEALQRPRPPVVTIMGHVDHGKTLLLDRIRQTNVVAGEAGGITQHIGAYQVEKDGRT
                     ITFIDTPGHEAFTAMRARGAEVTDIVVLMVAADDGVMPQTVEAINHAKAAEVPILVAI
                     NKIDREDADPNRVMQQLSEHGLVPERWGGDTIMVEVSALQNLGVDDLLEQLLVVAEIE
                     ELTANPEGRARGTVLEAYLDVGRGPVASVIVEDGTLRVGDPIVAGAAWGKVRALIDDT
                     GDQIKEATPSMPVQVLGFSDVPNAGDELRVTADQGTARTIGEAREQRYRSAGYAPTPS
                     ATPGAKLEDIFEQIQRGETATLNLILKADVQGSLEALTESLRKLERDEVKLSFVHRAV
                     GGITENDVQLAATSNATIIGFNVWPDRKVRELAGASKVEVRTYEIIYKVLEEIEAAMV
                     GMLAPEYEEIVTGDAEVRAIFRVPRVGNVAGCYVRNGVITRGSKVRFLRDGVVIWKGS
                     ITSLKRVKDDAREVAAGFECGIGLSDFQDLKEGDVIETFEEREIPRT"
     gene            2608..2979
                     /locus_tag="VF954_06430"
     CDS             2608..2979
                     /locus_tag="VF954_06430"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX9530754.1"
                     /translation="MRHRPVRLPGPQRGRRHRDLRGAGDPTHVAALSVELHLPQSRSL
                     KAKRAIVKPIVEGARHRYAVAAAEVDHQDKWQRCQLGIAAVAGSAGHVADVLDQVERW
                     VWSFPDVEVLSMERRWLDGGS"
     gene            3006..3389
                     /locus_tag="VF954_06435"
     CDS             3006..3389
                     /locus_tag="VF954_06435"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEX9530755.1"
                     /translation="MNELLREVVAETLERLADADERLALLTVTGVDAEPDLRSAKVYL
                     SSLPDDAGEALGEHRKSLQAAIGRQVRLKRTPQLSFAADPAVLMGERIEEVLRQVRAA
                     GDGEVRDSEVRESTVGDSERDDATD"
     gene            3376..>3704
                     /locus_tag="VF954_06440"
     CDS             3376..>3704
                     /locus_tag="VF954_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530756.1"
                     /translation="MQPIDDLEAAAAAIGAAGVVAMACHVDPDGDALGSMLALHHLCR
                     SQGKASVASWPEPFGYAPHYRFLPGLDLATKPSDFPEEPAVMVTFDCGALDRLGSLRA
                     TAARAATL"
BASE COUNT          528 a         1396 c         1336 g          444 t
ORIGIN      
        1 gccgtccccg ctttccgcgc cggccccgac ggccccgctt tccgcgccgg ccccgccgcc
       61 cgctccggcc ccgccggccg tcctgccgcc aaccccctcg cccggcgccg agcgccccgc
      121 ggcggcggcc cggcccgccg ctgctcccct ggagccggcc ccgcccaccc aggccccctc
      181 ccctgcgccc gccgaacgcc ccaccgtcgc cccgagcggt cgcgacccgc gtctggtgac
      241 cagtcgcccg gcgtccgagc agcccggcgc cgcccggccc gcgccggctt cattcgcgcc
      301 cggcggcgcc cggccaccgg cgggcgcgcc cgcgccccgt ggcgcgggcg gcccggccca
      361 cgcccctggc caccgcccgg cggcggccgc ggccgggccc ggcggctcca gtgccgcccc
      421 tcctcgggac acccgcccgc ccgtgagcgc gagcggcaag cccatccctc cccctcccgg
      481 catgcggcct ccactgtcgc cgagcggccg accagtgccg cccccgcccc gatcgtcgac
      541 gggccgcccc atcccaccgc ctcctggcca ggggggccgc accacgccgg ccggccgccc
      601 cgccggcggc ggcggaggag ccgggcggcc ggcaggcggt ggctatggcg gcccgcggcc
      661 cggcggcggc ggtggcgggc ggcccgcggg cggtggcttc ggaggccgag gcccgggtgg
      721 cggcccaggc ggattcggag gcggcgcccc tggtcgcggc ggtcccgccc aggcgggcac
      781 ccgcggcggg ccgggcgcag gtggcggacg gggccggcct ccccagcggc ggccgcggcg
      841 ccggcggcgc aacatggagg agctcgagcc caccaccctc accacctaca cgccctccaa
      901 cgccccggtc cccgagggcg agaccatcat cgagcgggga tcgaccgccc aggacgtcgg
      961 gcccaagctc aaccgctcgg ccggtgacgt ggtgcgcttc ctgctcctcc agggtgagat
     1021 ggtgaccgcc acccagtcgc tgtcggacga catgatcgag ctgttcgcgg ccgagctggg
     1081 cgcgaccgtg cacctcgtcg atcccggcga ggagaaggag gccgccctcc aggccaagta
     1141 cttcgacgac gacgacgagg gcgacgaggc cctgcagcgg ccgcgccccc cggtcgtcac
     1201 gatcatgggt cacgtcgacc acggcaagac gctgctgctg gaccgcatcc gccagaccaa
     1261 cgtcgtggcg ggcgaggccg gcggcatcac ccagcacatc ggtgcctacc aggtcgagaa
     1321 ggacggccgg acgatcacgt tcatcgacac gcccggtcac gaggccttca cggccatgcg
     1381 cgcccgcggc gcggaggtga ccgacatcgt ggtgctgatg gtggccgccg acgacggggt
     1441 catgccccag acggtcgagg ccatcaacca cgccaaggcc gccgaggtcc cgatcctggt
     1501 cgccatcaac aagatcgatc gcgaggacgc cgacccgaac cgggtgatgc agcagctctc
     1561 cgagcacggg ctggtcccgg agcggtgggg gggcgacacg atcatggtcg aggtctcggc
     1621 cctccagaac ctgggcgtcg acgacctgct cgagcagctg ctcgtggtgg ccgagatcga
     1681 ggagctgacc gccaatccgg agggtcgggc ccggggcacg gtgctcgagg cctacctgga
     1741 cgtcggccgg ggacctgtgg ccagcgtcat cgtcgaggac gggacgcttc gcgtcggtga
     1801 cccgatcgtg gccggcgcgg cgtggggcaa ggtccgggcc ctcatcgacg acacgggcga
     1861 ccagatcaag gaggcgacgc cctccatgcc cgtccaggtg ctcggcttca gcgacgtgcc
     1921 caacgccggg gacgagctcc gtgtcacggc cgaccagggc acggcccgga ccatcgggga
     1981 ggcccgggag cagcgctacc gctccgccgg gtacgccccg acgccgtcgg ccactcccgg
     2041 cgccaagctc gaggacatct tcgagcagat ccaacgaggc gagacggcga cgctcaacct
     2101 catcctcaag gccgatgtcc agggctcgct ggaggctctc accgagagcc tgcgcaagct
     2161 ggagcgcgac gaggtcaagc tcagcttcgt gcatcgggcc gtcggtggca tcaccgagaa
     2221 cgacgtgcag ctggcggcca cctccaacgc gaccatcatc ggcttcaacg tgtggcccga
     2281 ccgcaaggtc cgcgagctgg ccggcgccag caaggtcgag gtcaggacct acgagatcat
     2341 ctacaaggtg ctcgaggaga tcgaggctgc catggtcggc atgctcgccc ccgagtacga
     2401 ggagatcgtc acgggcgacg ccgaggtgcg ggccatcttc cgggtgcccc gggtcggcaa
     2461 cgtggccggc tgctacgtcc gcaacggggt catcacgcga ggctccaagg ttcgcttcct
     2521 ccgcgacggc gtcgtcatct ggaagggctc gatcacgtcg ctcaagcggg tcaaggacga
     2581 cgcgcgcgag gtggccgccg gcttcgagtg cggcatcggc ctgtccgact tccaggacct
     2641 caaagagggc gacgtcatcg agaccttcga ggagcgggag atcccacgca cgtagcggcc
     2701 ctgtcggtcg agctgcatct gccccagtcc cggtcgctca aggccaagcg ggccatcgtc
     2761 aagcccatcg tcgagggagc ccgccaccgg tacgccgtcg cggcggcaga ggtcgaccac
     2821 caggacaagt ggcaacggtg ccagctcggg atcgccgccg tggccggctc ggccggtcac
     2881 gtcgccgacg tgctggacca ggtggagcgc tgggtgtggt ccttcccgga cgtcgaggtg
     2941 ctgtcgatgg agcggcggtg gctggacggc gggtcgtgag ccaccgatac ccgcgcaccg
     3001 cccgggtgaa cgagttgctc cgtgaggtgg tcgccgagac cctggagcga ctcgccgatg
     3061 ccgacgagcg gctggcgctg ctgacggtga ccggcgtgga cgccgagccg gacctgcgca
     3121 gcgccaaggt gtacctgagc tcgctgcccg acgacgccgg cgaggccctg ggcgagcatc
     3181 gcaagagcct gcaagcggcc atcggccgcc aggtgcgcct gaagcgcacg ccccaacttt
     3241 ccttcgcggc cgatcctgcg gtactcatgg gggaacggat agaagaggtg ctgcgccagg
     3301 tgcgagcagc cggggacggc gaagtcaggg acagcgaagt cagggaaagc acggttgggg
     3361 acagcgagag ggacgatgca accgattgac gatctcgagg cggccgcggc ggccatcgga
     3421 gccgccggcg tggtcgccat ggcctgccac gtcgacccgg acggggacgc gctcggctcc
     3481 atgctcgccc tgcaccatct ctgccggtct cagggcaagg cgtcggtggc gtcgtggccg
     3541 gagccgttcg gttacgcccc ccactaccgg ttcctgccgg ggctggacct ggcgaccaag
     3601 ccctcggact tcccggagga gccggccgtc atggtcacct tcgactgcgg ggcgctcgat
     3661 cgcctcggca gcctccgggc caccgcggcc cgggctgcga cgct
//
LOCUS       DASWGG010000355         3961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_1933320, whole genome shotgun sequence.
ACCESSION   DASWGG010000355 DASWGG010000000
VERSION     DASWGG010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933320"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VF954_06445"
     CDS             complement(<1..563)
                     /locus_tag="VF954_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530757.1"
                     /translation="MAVSRLVSRPAPAAAAMAALALVAPALAACGGDRTGGPQGTPTE
                     VVLGAIDRTQALGGATFQIGVGEVAAGTGRVVWRSVPAGSPGGPSVVITRVSLSLHPV
                     RAAVSGGPDTADVVLIGPDAFVRSSATGGRWVTGPAAALARQLAARAPAVAEVLARPG
                     AGLALDTLRGASSVLTYGGAEVRGASTL"
     gene            671..1573
                     /locus_tag="VF954_06450"
     CDS             671..1573
                     /locus_tag="VF954_06450"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9530758.1"
                     /translation="MAHHPHTSERSLSGPDATRTGSSAAGSSPAPELRWWKEALYTLG
                     FYVVYSVVRNTQGSGSVSKARAFHNARTIIALERHLGLFHEQAVQHAFVHWRWFIEGW
                     DVFYGAAHFVVTAVAIIWMFRAFKQRYPLWRNTLACTTALALVGFAFFPLMPPRLLAT
                     MPGTHFGLVDTLKTVGGLWSFDSGTMQKVSNQYAAMPSLHFAWSTWCALVLFPQVRSR
                     RAKLAVASYPGLTLFAVVVTANHFVLDAVGGGLVLGAGYLAATGLTLITRPRAARARR
                     GAGMEPFQEATDEPDDDARRVAAG"
     gene            1717..3756
                     /gene="dxs"
                     /locus_tag="VF954_06455"
     CDS             1717..3756
                     /gene="dxs"
                     /locus_tag="VF954_06455"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX9530759.1"
                     /translation="MILDTISSPADLKELGPDQLLLLAGEIREFIVDAVTRTGGHLGS
                     NLGAVELTLAVHRVFDSPRDIILWDTGHQAYVHKLVTGRKDGFATLRQAGGLSGYPSR
                     KESEHDWVENSHASTILSYAHGLAAAVEQAGADRRVIAVVGDGAMTGGMAFEALNNLG
                     HSGRRVIIILNDNGRSYAPTVSRLGESLTRLRANPSYVQTRNWMEQALRDLPAVGSYA
                     QLGMQGLRAAFREVLEPRDFFEDLGVRHIGPIDGHDIAGMEQALRDAVANDGPIVVHV
                     LTQKGRGYGPAEDDEIQCLHDLKVQTAAPGSVPASCTEAFSSTLLDLADRHPQIVAIT
                     AAMAGPTGLLPFQARYPDRFFDVGIAEQHAVTAAAGMAMGGLRPVVAVYSTFFSRAFD
                     QANLDVGLHGLPVLFALDRAGITGDDGPSHHGVLDLVLTLTIPGMTIFAPSSAEDLEA
                     MLRTAVDLDGPAVIRWPKNAPVAPAHVLAELTTAGRGLDARRLRPAAGGSPVSGAAGS
                     FRSYGSVCILAVGKMVAAALEAADELTAEGIDTTVWDVRVVKPLDPAMLADAARHQLV
                     VTVEDGMRVGGAGSAIVDAIAALSPRRQAPPALVLGTPSAYIPQGKANQLLADLGLDG
                     AGVAAATRAALGAATRAALGAATRAAVGPATSASTVPASASAVGTSRRDAVIQLD"
BASE COUNT          547 a         1470 c         1400 g          544 t
ORIGIN      
        1 agcgtcgagg caccccgaac ctccgcgcct ccgtaggtca acacgctcga ggcaccccgg
       61 agggtgtcga gggcgagccc agcgccgggg cgggcgagca cctcggctac ggccggggcc
      121 cgggcggcaa gctgtcgggc cagggccgcg gccggccccg tcacccaccg accgccggtg
      181 gccgaggacc ggacgaaggc gtccgggccg atgagcacga catcggccgt gtcggggccg
      241 cccgacacgg ctgcccggac cgggtggagg ctgagcgaga ccctggtgat caccacggac
      301 ggcccgcccg gcgatccggc cggcacgctc cgccacacca cccggcccgt cccggccgcc
      361 acctcgccca cgccgatctg gaaggtcgcg ccccccaggg cctgggtccg gtcgatggcg
      421 ccgagcacga cctccgtcgg cgtaccctga gggccaccgg tgcggtcgcc cccgcaggct
      481 gcgagggccg gggcgaccag agccagggcc gccatggcag ccgcagcagg tgccggccgg
      541 gacaccagcc gggagacagc catacatcct ccatagggga gatcttggag cgtcgcatta
      601 cgctgagtgg ttcgcagacc ctaagcggtc ctgacccgag gagacaccgc atgcgccggg
      661 tgaaggcggc gtggcccatc atccgcatac ctctgagcgc agcttgagcg gcccggacgc
      721 gaccagaact ggctcgagcg cagccgggag cagcccggca ccggagctgc ggtggtggaa
      781 ggaggccctg tacaccctgg gcttctacgt cgtgtacagc gtggtccgca acacccaggg
      841 ctcgggctcg gtatcgaagg cgcgcgcctt tcacaacgcc cggacgatca tcgctctcga
      901 acgccacctc ggcctgttcc acgagcaggc cgtccagcac gccttcgtgc actggcggtg
      961 gttcatcgaa gggtgggacg tgttctacgg cgcggcgcac ttcgtggtca cggccgtcgc
     1021 catcatctgg atgttccgag ccttcaagca gcgctacccg ctgtggcgca acaccctggc
     1081 ctgcacgacg gcgctcgccc tggtgggctt cgccttcttc ccgttgatgc cgccccgcct
     1141 gctggcgacg atgccgggta cccacttcgg attggtggac acgctcaaga cggtcggggg
     1201 gctctggtcc ttcgacagcg gcacgatgca gaaggtctcc aaccagtacg ccgccatgcc
     1261 gagcctccat ttcgcctggt ccacgtggtg cgcgctggtc ctgttccccc aggtgcggtc
     1321 gcgccgcgcc aagttggcgg tcgcctccta ccccggcctc acgctgttcg ccgtcgtggt
     1381 aacggccaac cacttcgttc tcgatgccgt gggaggcggg ctcgtgctgg gcgccggcta
     1441 cctggcggcg acgggcctca ccctgatcac ccgcccgcgg gcggccaggg ctcgccgagg
     1501 cgcgggcatg gagccgttcc aggaggcgac cgacgagccg gatgacgacg cccgtcgggt
     1561 ggccgccggg tgaggaccct cacggtcgcg ggcctccgcg agcggtccgc agcctgcccg
     1621 gcccggctcc aggtgcgggg tccgaccggg ctacccttgg ccgccggagg tccgcccgac
     1681 ccatgatcct cgactgcaca tgatcctcga cgccacatga tcctcgacac catcagcagc
     1741 ccggccgacc tgaaggagct gggccctgac cagcttctgc tgctcgcggg ggagatccgt
     1801 gagttcatcg tggacgccgt gacccgcacc ggcggacacc tgggatccaa cctcggggcc
     1861 gtggagctga cgctcgccgt gcaccgggtg ttcgactcgc cccgcgacat catcctgtgg
     1921 gacaccggcc accaggccta cgtgcacaag ctggtcacgg gacgcaagga cggcttcgcc
     1981 accctgcgcc aggccggcgg gctgtcgggt tacccgtcgc ggaaggagtc cgagcacgac
     2041 tgggtcgaga acagccacgc ctcgaccatc ctttcctacg cccacggcct ggccgcggcc
     2101 gtcgagcagg cgggtgccga ccgccgggtg atcgccgtgg tgggcgacgg ggccatgacc
     2161 ggaggcatgg cgttcgaggc gctcaacaac ctgggacatt ccgggcgccg ggttatcatc
     2221 atcctcaacg acaacggccg cagctatgcg cccacggtgt cccgcctcgg ggagagcttg
     2281 acccggctgc gcgccaaccc ctcctacgtc cagacccgga actggatgga gcaggcgctg
     2341 cgcgacctgc cggcggtggg ctcgtacgcc cagctgggga tgcagggact gcgggcggcg
     2401 ttccgcgagg tgctggagcc ccgtgacttc ttcgaggacc tgggagttcg acacatcggt
     2461 cccatcgacg gccacgacat cgccgggatg gagcaggccc tgcgggacgc ggtggccaac
     2521 gacgggccca tcgtggtgca cgtcctaacc cagaagggcc ggggctacgg cccggccgag
     2581 gacgacgaga tccagtgcct ccacgatctc aaggtgcaga ccgcggcgcc gggctcggtg
     2641 ccggccagct gcaccgaggc cttcagctca accctcctcg acctggcgga caggcacccc
     2701 cagatcgtgg ccatcaccgc ggcgatggcc gggccgacgg ggctgctccc gttccaggcc
     2761 aggtaccccg accggttctt cgacgtcggc atcgccgagc agcacgccgt caccgcggcc
     2821 gccgggatgg cgatgggcgg gctgcgaccg gtggtggcgg tgtactccac gttcttctcc
     2881 cgggcgttcg accaggccaa cctggacgtc ggcttgcacg ggctgccggt gctgttcgcg
     2941 ctggaccggg cgggcatcac cggcgacgac gggccgtccc atcacggtgt cctggacctg
     3001 gtcctcaccc tgacgatccc gggcatgacg atcttcgcgc cgtcgtcggc cgaggacctc
     3061 gaggccatgc tgcgcaccgc ggtcgacctg gacggcccgg ccgtcatccg ttggcccaag
     3121 aacgctccgg tcgcgccggc gcacgtgctt gccgagctca ccacggcggg gcggggcctc
     3181 gacgcccgcc gcctccgccc ggcggcgggc ggctcccccg tgtctggcgc ggcggggtcc
     3241 ttccggtcgt acggttcggt gtgcatcctc gccgtgggca agatggtggc cgccgctctg
     3301 gaggctgccg acgagctcac cgccgaaggg atcgacacga ccgtgtggga cgtgcgggtc
     3361 gtcaaaccgc tcgacccggc catgctcgcc gacgccgccc gccaccagct ggtggtcacg
     3421 gtggaggacg gcatgcgcgt cggcggcgcg ggatcggcca tcgtggacgc catcgccgcc
     3481 ctgagccccc gccggcaggc gcccccggcg cttgtgctcg ggaccccgtc ggcgtacatc
     3541 ccgcagggaa aggccaacca gctcctggcc gatctcggcc tggatggggc aggcgtggcg
     3601 gccgcgacgc gcgccgccct gggcgcggcc acgcgcgccg ccctgggcgc ggccacgcgc
     3661 gccgccgtgg gcccggccac gagcgcctcc acggtgccgg cctcggcctc cgcggtgggt
     3721 acctcccggc gcgacgccgt catccagctc gactagaccg cgcgccccga cccggcggcc
     3781 ggctccttac agcgcggacg gcgttctggt gacggttgga agggcatggc ccgcatgagc
     3841 gtcaccagaa tccgcgtcgg ccggcgccgg ggccgcgcga gactgcctcc gtggatgcac
     3901 cgatctacct cgaggcgatc cggcgggaga gcgccgcctt tgccgccgcg gccggccgag
     3961 a
//
LOCUS       DASWGG010000356         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2891085, whole genome shotgun sequence.
ACCESSION   DASWGG010000356 DASWGG010000000
VERSION     DASWGG010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2891085"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1743)
                     /locus_tag="VF954_06460"
     CDS             complement(<1..1743)
                     /locus_tag="VF954_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX9530760.1"
                     /translation="MLVVLAVAFAAVTARLVYIQGPAAARYVALGENERVRTVALPAE
                     RGALFDREGQDLALSIPQTTVWADPHLVTDPMGEAGRLAPVLGVPAPDLQALLSKDAG
                     FVYLARKVTDPVASQVRALKLDGIAYLDEPERFLPAGNLAAPIVGQVGLDNNGLSGLE
                     LQYDKQLSGQPGELIVERDPKGHDIPGGLHRFQPATKGQDLILTVDRSLEYQTEQALA
                     TEIQHDKAKGGMAIVMDSKSGEILAMANLVAGPNQGDPPQPAPSDTALTAVYEPGSVN
                     KVVTLSGALEEGIIHPDDHLMVPDNMRVGDYTFRDNEAHPTESMSITDILANSSNIGT
                     ITIAQKLGKDKIDHYLRAYGFGKPTGLGYPGESAGLLLDPAKWSGSSIGSVPIGQSVA
                     VTAMQMIAAYNTIADGGTYIAPKLVKGTVDAHGVRHATPPSVRHRVISARTAKQVTAM
                     LNEVVRVGTGTAAAIDGYTVAGKTGTARVPENGGYKAGVYVASFAGFVPSQDPALTAM
                     VVIDEPQTMYGGTASAPVFAAIARYALREFRIPPPPAVPVANVPAASQATAGVDEPVP
                     PAPAAVTSPPATAPP"
     gene            complement(1911..>2266)
                     /locus_tag="VF954_06465"
     CDS             complement(1911..>2266)
                     /locus_tag="VF954_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530761.1"
                     /translation="RLDSQVAAQQSQYDRLRLQIATLESPARIVAAAQERLGMVPPPS
                     VRYLSPSAGAPPSPAKTAVTHPPAVASSTTTPRQGTSSPPTTAAPRATSSPSTTAPPR
                     PKPTTAPPTTAPRHP"
BASE COUNT          267 a          739 c          924 g          336 t
ORIGIN      
        1 gggcggcgcg gtggccggcg gcgaggtgac ggccgcgggg gccggcggca cgggctcgtc
       61 cacgccggcg gtcgcctgag acgcggccgg gacgttggcc accgggacgg cgggcggcgg
      121 cgggatgcgg aactcacgca gggcgtagcg ggcgatggcg gcgaacaccg gcgccgacgc
      181 cgtcccgccg tacatcgtct ggggctcgtc gatcacgacc atggcggtca gggcggggtc
      241 ctgcgacggg acgaagccgg cgaagctggc cacgtagacg cccgccttgt agccgccgtt
      301 ctcgggcacc ctggcggtgc cggtcttgcc ggcgacggtg tagccgtcga tggccgctgc
      361 cgtgccggtc ccgacccgga ccacctcgtt gagcatggcc gtgacctgct tcgccgtccg
      421 ggcggagatg acccggtggc ggaccgacgg tggggtggcg tggcggaccc cgtgcgcgtc
      481 aacggtgcct ttcaccagct tcggggcgat gtaggtgccg ccgtcggcga tggtgttgta
      541 ggccgcgatc atctgcatgg ccgtgacggc cacgctctgg ccgatcggga cggagccgat
      601 ggagctgccc gaccacttgg cggggtcgag gagcagaccc gccgactcac cggggtaacc
      661 gaggccggtc ggctttccga agccgtaggc ccgcagatag tggtcgatct tgtccttgcc
      721 cagcttctgg gcgatggtga tcgtcccgat gttcgacgag ttggcgagga tgtcggtgat
      781 cgacatgctc tccgtggggt gggcctcgtt gtcgcggaag gtgtagtcgc cgactctcat
      841 gttgtccggc accatgagat ggtcgtcggg gtggatgatc ccctcttcca gggcaccgga
      901 gagggtcacg accttgttga ccgagcccgg ctcgtacacg gcggtcagcg cggtgtcgga
      961 gggggcgggt tgcggcgggt cgccctggtt gggcccggcc accaggttgg ccatggccag
     1021 gatctcgccg ctcttgctgt ccatcacgat cgccatgccg cccttggcct tgtcgtgctg
     1081 gatctcggtc gccagggcct gctccgtctg gtactcgagg gaccggtcga cggtgaggat
     1141 gaggtcctgg cccttggtcg cgggctggaa gcggtgcagc ccgcccggga tgtcgtgccc
     1201 cttggggtcg cgctcgacga tcagctcgcc cggctggccg gagagctgct tgtcgtactg
     1261 gagctcgagc ccggacaggc cgttgttgtc gagcccgacc tgaccgacga tgggcgcggc
     1321 caggttgccg gccggcagga agcgctcggg ctcgtcgagg taggcgatcc cgtcgagctt
     1381 gagcgccctg acctgcgacg ccaccgggtc ggtcaccttg cgggccaggt agacgaaccc
     1441 ggcgtccttg ctcaacaggg cctgcaggtc cggcgcgggg accccgagga cgggcgccag
     1501 cctgccggcc tcgcccatcg ggtcggtcac caggtgcggg tcggcccaga cggtggtctg
     1561 cgggatggag agggccaggt cctggccctc ccggtcgaac agcgcccccc gctcggcggg
     1621 cagcgcgacc gtgcgcaccc gctcgttctc accgagagcg acgtacctgg ctgcggccgg
     1681 tccctggatg tacaccaggc gggccgtcac cgccgcgaag gccacagcca gcacgacgag
     1741 catgccggtc agccgccggg tcgcgcccga gcggcccggc ctgggcgcca cacgcggcgc
     1801 gcccggtcca gggggagaaa gggtggcccg ttgccccgca gcccgcggca ccggggcccg
     1861 gcgtggccgg agcggccggg ccggggcgtg cgcggtggcc gtgggcgggg tcacgggtgc
     1921 cgcggcgcgg tcgtgggcgg cgcggtcgtc ggcttgggcc tggggggggc ggtcgtggac
     1981 ggagacgagg tggcccgggg cgcagcggtc gtggggggag acgaggtgcc ctggcggggg
     2041 gtggtcgtgc tggatgccac cgccggcggg tgggtcaccg ccgtcttggc cggggagggc
     2101 ggggcaccgg cggagggaga caggtaccgg acgctggggg gagggaccat cccgaggcgc
     2161 tcctgggccg ccgccacgat gcgggccggc gactcgaggg tggcgatctg gagccggagc
     2221 cggtcgtact gcgactgctg cgccgccacc tggctgtcga gccggt
//
LOCUS       DASWGG010000357         4955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_965064, whole genome shotgun sequence.
ACCESSION   DASWGG010000357 DASWGG010000000
VERSION     DASWGG010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965064"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VF954_06470"
     CDS             complement(<1..1145)
                     /locus_tag="VF954_06470"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9530762.1"
                     /translation="MAVRRRVLADLTPLRASREFRLLWGGQLVNFLGTQLTVVAVPFQ
                     VYLLTRSSLAVGLVSLAQLVPLAVGSLLGGSMADAFDRRRVLLVTQVLLAAMSTGLAL
                     NAAGRHPALWPLFVLTSVSAGLSGIDSPTRNAVLPNLLDRATLPAAFALWQVLIQTGQ
                     VAGPALAGLLVGHVGLAPVYWIDVGSFAVAFASVAALPALPPQGGGRRAGFRSIGEGL
                     RYLRGHQALQGTFVIDLDAMIFGMPRALFPALGTRYFGGGASTVGLLYAAPGAGALVG
                     AVLTGWVSIVRRQGRAVVAAVMVWGAAIALFGVSRWLPLALALLGVAGAADVVSAVFR
                     NTILQLSVPDSLRGRLSAVHIAVVTGGPRIGDAEAGAVAALAGNQLSV"
     gene            1285..1521
                     /locus_tag="VF954_06475"
     CDS             1285..1521
                     /locus_tag="VF954_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530763.1"
                     /translation="MVRLSTWLRSLGNDGAIANALRASAAPRRQADALEAQLAGVPCT
                     DSAGRTDSVGRTDGTRRTDGTRRTAAPSPTRAVA"
     gene            complement(1591..1875)
                     /locus_tag="VF954_06480"
     CDS             complement(1591..1875)
                     /locus_tag="VF954_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530764.1"
                     /translation="MSLAIGEQAPGAPVDALGTGAATPQISAVVARPHAGFDPALAGM
                     AVTPPRKRIPAGARHGLRYRCRRCDVYGYLAPGEHVTCWACDDGAMVEPR"
     gene            complement(1880..2056)
                     /locus_tag="VF954_06485"
     CDS             complement(1880..2056)
                     /locus_tag="VF954_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530765.1"
                     /translation="MTNPGIAAPLSRARHQKILEATTLVQERATEALAEIDQALAEAP
                     PSSARRRSTAPSPA"
     gene            2234..3103
                     /locus_tag="VF954_06490"
     CDS             2234..3103
                     /locus_tag="VF954_06490"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX9530766.1"
                     /translation="MDRSTGIVRWEERPSLRRPVLIAAFEGWNDAGDAATSAARYLAR
                     TWEARTFARFDPEEFYDFTSTRPQVRLVNGETRQIDWPDNEWQAAPVPGGSHDIVFLH
                     GVEPQLRWRTFCQSVVDIVKESSVEMVFTLGALLADVPHSRPVRVSGSATNRDLVDRL
                     HLQRSRYEGPTGIVGVLHESLARAGVPSASLWASVPHYVSQTPSPKAALALVERTAQL
                     LGARVEPLELQIAASAYERQVTEVVEGDDDVAAYVRRLEQADDDEGADPNTVMTGDAL
                     ADEVERFLRDPDI"
     gene            complement(3322..4563)
                     /locus_tag="VF954_06495"
     CDS             complement(3322..4563)
                     /locus_tag="VF954_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9530767.1"
                     /translation="MTDIPAFLHPFARPAAGRDAFIRIVSAEGSSVIDDAGKRYVDAL
                     ASLWYCNVGHGRAEIIDAIASSMRSLDVFHTFDRFTNPPAEELADILRRLAPMPDARA
                     FFTCSGSEAVDSAIKLARLAHVAAGRPERTLVVSRTPSYHGVTFGAMSATGLAPNQAG
                     FGPLVPDVVQVPYDDLAALDDLLADQGERLAAIIAEPVVGAAGVYPPPPGYFAGLRER
                     ADRHGGFLISDEVICGFGRLGRWWGIQHYGVTPDMVTFAKGVTSGYQTVGGVLVGAAV
                     RGPLEADASLVLRHGHTYSGHPAGCAAGVANLAIVEREELWTRAAPVGERLRAGLEAV
                     VDGDAVLGVRGDGAVWAIGLGGGVGALDVREELLARGVIARPIGADTVAFCPPLVIGD
                     ADLDHIAEAVAGAVKAVAAGR"
     gene            4611..>4955
                     /locus_tag="VF954_06500"
     CDS             4611..>4955
                     /locus_tag="VF954_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530768.1"
                     /translation="MAAPILTPTCVWRATPGVIVALDDRFGEPTDSYLNGSQVWLRPD
                     GPGGIVIEWRLHPVAGFRRPATAGTYELFPVTALAVARGQPPPAPIAELWDGLEAFAA
                     YGDEVEPSPLAAV"
BASE COUNT          712 a         1807 c         1830 g          606 t
ORIGIN      
        1 acggagagct ggttgccggc cagcgccgcg accgcacccg cctcggcgtc gccgatccgc
       61 ggcccgcccg tgacgactgc gatgtgcacc gccgacaggc gcccgcggag ggagtccggg
      121 acgctgagct ggaggatggt gttgcggaac acggccgaca ccacgtcggc cgccccggcg
      181 acgccgagca gcgccagggc cagcggcagc caccgtgaca cgccgaacag ggcgatggcg
      241 gcgccccaaa ccatcaccgc ggccacgacc gcccggccct gccggcgtac gatgctgacc
      301 cacccggtca ggaccgcgcc caccagcgcg cccgcacccg gagcggcgta cagcaggccg
      361 accgtcgagg ccccgccccc gaagtaccgg gtgcccaggg cggggaacag ggcacgtggc
      421 atgccgaaga tcatggcgtc cagatcgatg acgaacgtgc cctggagggc ctggtggccc
      481 cgcaggtacc gcagcccctc gccgatcgag cggaacccgg cccgccgccc cccgccttga
      541 ggcggcagcg ccggaagggc ggccaccgag gcgaaggcca ccgcaaagct gcccacgtcg
      601 atccaataga cgggggccag ccccacgtgg ccgacgagga gcccggccaa ggcggggccg
      661 gcgacctgcc cggtctggat gaggacctgc cacagggcga aggcggccgg cagggtggcg
      721 cggtcgagga ggttcggcag caccgcgttt cgtgtcggcg agtcgatccc ggagagccct
      781 gccgagacgg acgtcaggac gaacagcggc cacagcgccg ggtggcggcc ggccgcgttg
      841 agggcgagcc cggtgctcat ggccgcgagc agcacctggg tgaccaggag gaccctgcgc
      901 cggtcgaagg cgtcggccat cgagccgccg agcagggacc ccactgcgag ggggacgagc
      961 tgggccaggc tcaccagccc gacggcgagg gacgagcgcg tgagcaggta cacctggaac
     1021 ggcacggcca ccaccgtgag ctgggtaccc aggaagttga ccagttggcc gccccacagc
     1081 aggcggaact cccgcgacgc ccggaggggc gtgagatcgg cgagcacccg gcggcggacg
     1141 gccaccgcca gaggctagga gggtggcggg accattccgc aatcgtccgg tcaggtgccc
     1201 ggttcaggca gaagtcatca aatcgtcaca ccgtcgcccc ttgaccgtag cgtggcggac
     1261 acggcggctt cgtacccttt ggtcatggtc cggctgagca cgtggctccg gtctctcggc
     1321 aatgacggcg ccattgccaa cgccctccga gccagcgccg cgccccggcg gcaagccgac
     1381 gccctggagg cccagctggc cggcgtgccg tgcaccgaca gcgccggccg caccgacagc
     1441 gtcggccgca ccgacgggac ccgacgcacc gacgggaccc gacgcaccgc ggcgccgtcg
     1501 cccacccgcg ccgtcgcgta ggtactcctg acgccgctct cggcaggtcg gaccaggtcc
     1561 aactaaccgg gtccgacgaa ccagctcgaa ctagcggggc tcgaccatgg cgccgtcgtc
     1621 gcacgcccag cacgtgacgt gctcgccggg cgcgagatag ccgtagacgt cgcagcgccg
     1681 gcagcggtac cgaaggccat ggcgggcccc ggcggggatg cgcttcctcg gcggggttac
     1741 cgccatgccg gcgagcgcgg gatcgaaccc ggcgtggggc cgggccacga ccgcggagat
     1801 ctggggggtc gcagccccgg tgccgagcgc gtcgacgggc gcgcccggtg cctgctcacc
     1861 gatcgcgagg gacacgtcgc taggcggggg acggcgccgt cgaccggcgc ctggcggagg
     1921 aggggggtgc ctccgccagg gcctgatcga tctcggccag cgcttcggtc gccctctcct
     1981 ggacgagggt ggtggcctcg aggatcttct ggtgccgggc cctggaaagg ggcgccgcga
     2041 tgcctggatt ggtcatggcc cctcctcgcg tctcggccac cggagcctgt ggcacctaca
     2101 ccagagaagt cggccgatcg agcggattgg tgaagcgcga cgtgcgaaat tgacgcgccc
     2161 ggactagtcc gcccattgcc ggaggcggag cgacgatgct cgggctctcg ccaccccgcg
     2221 ttagcctcgt tcgatggacc gttcgacggg catcgtccgg tgggaagagc ggccgtccct
     2281 gcgccggccc gtcctgatcg ccgccttcga gggatggaac gacgcgggcg acgcggccac
     2341 cagcgcggcc cgctacctgg ccaggacatg ggaggccagg acgtttgcga ggttcgaccc
     2401 cgaggagttc tacgacttca cctcgacccg gccccaggtg cggctggtga acggcgagac
     2461 gcgccagatc gactggcccg acaacgaatg gcaggccgcc ccggtccccg gtggcagcca
     2521 cgacatcgtg ttcctgcacg gcgtggagcc ccagctgaga tggcggacct tctgccagtc
     2581 ggtcgtggac atcgtcaagg agtcctcggt cgagatggtg ttcacgctcg gcgccctgct
     2641 cgccgatgtt ccccacagcc gcccggtccg ggtgtccggg tcggcgacca accgtgactt
     2701 ggtcgatcgg ctgcacctcc agcgctcccg gtacgaagga ccgaccggca tcgtgggcgt
     2761 gctgcacgaa tccctcgccc gggccggggt ccccagcgcg tcgttgtggg ccagcgtccc
     2821 ccattacgtg tcccagacgc cgtcgcccaa ggccgccctc gccttggtcg agcgcaccgc
     2881 ccagctcctc ggcgcccggg tcgagcccct cgagctgcag atcgcggcgt cggcctacga
     2941 acgccaggtg accgaggtcg tggaaggaga cgacgacgtg gccgcctatg tgcggcgcct
     3001 cgagcaggcc gacgacgacg agggcgcgga tcccaacacc gtgatgaccg gcgacgccct
     3061 ggccgacgag gtggagcgct tcctgcgcga cccggacatc tgaccggccc ggagcgcgct
     3121 ggggcgacca gctacgaggc cacgccgccc tcggcgggga gcggagtcgt ccagatgccg
     3181 gttggtcgcg tgggcctcgg ctgcccggct gcccggttgc ccggttgggc cggttggccg
     3241 cctgcccgtg cggtcagagc aggacgatgt cggcgtggct ggttcgggca cgtcccgggc
     3301 ggtggtcgcc acgccgcaga gctatcgccc ggccgcgacc gccttgaccg cgcccgcgac
     3361 cgcctccgcg atgtggtcca ggtcggcgtc gccgatcacc agcggcgggc agaaggcgac
     3421 ggtgtcggcg ccgatgggcc gggcgatcac ccccctggcg agcagctcct cccgcacgtc
     3481 gagcgccccg acccccccgc ccagcccgat cgcccatacg gcgccgtcgc cgcgcacgcc
     3541 gaggaccgcg tctccgtcga ccacggcctc cagcccggct cgaaggcgtt cccccacggg
     3601 cgcggcgcgg gtccacagct cctcgcgctc cacgatggcc aggttggcca ccccggccgc
     3661 gcagcctgcc gggtgcccgc tgtaggtgtg gccgtggcgc agcacgaggg aggcgtcggc
     3721 ctcgagcggg ccccggacag cggcgccgac gagcaccccg ccgacggtct ggtaccccga
     3781 ggtgacccct ttggcgaagg tgaccatgtc gggcgtgacg ccgtagtgct ggatgcccca
     3841 ccatcggccc aaccggccga agccgcagat cacctcgtcg gagatgagga agccgccgtg
     3901 gcggtcggcg cgttcccgga gaccggcgaa gtagccgggt ggcggcgggt acaccccagc
     3961 ggcgcccacc accggctcgg cgatgatggc cgccaggcgt tcgccctggt cggcgagcag
     4021 gtcgtcgagg gcggccaggt cgtcgtacgg gacctggacg acgtcgggga cgaggggccc
     4081 gaagccggcc tggttggggg ccagcccggt cgcagacatg gccccgaagg tgacgccgtg
     4141 atagctcggc gtgcggctca ccaccagggt gcgctcggga cggcccgccg ccacgtgcgc
     4201 caggcgggcc agcttgatgg cgctgtccac ggcctcggac ccgctgcagg tgaagaacgc
     4261 cctggcgtcg ggcatcggcg cgaggcgccg gaggatgtcg gccagctcct cggccggcgg
     4321 gttggtgaag cggtcgaagg tgtggaacac gtcgagggac cgcatcgacg acgcgatggc
     4381 gtcgatgatc tcggcccggc cgtggcccac gttgcagtac cacaggctgg ccagggcgtc
     4441 cacgtaccgc ttgccggcgt cgtcgatcac cgacgagccc tcggccgaga cgatcctgat
     4501 gaaggcgtcg cgacccgcgg ccggccgggc gaaggggtgg aggaacgcgg ggatgtcggt
     4561 cactggtcgc ccatcctacg gacggcgccg gccttcgtag gctgtcccca gtggccgcgc
     4621 cgatcctcac cccgacttgc gtctggcggg ccacgccggg tgtgatcgtc gccctcgacg
     4681 accggttcgg ggagccgacc gactcctatc tcaacgggtc ccaggtctgg ctgcgccccg
     4741 acggcccggg ggggatcgtg atcgagtggc gcctgcaccc cgtggccggg ttccgccgcc
     4801 ccgcgaccgc cggcacctac gagctgttcc cggtgaccgc gcttgcggtg gcgcggggcc
     4861 agcccccgcc ggcgccgatc gccgagctgt gggacggcct cgaggccttc gcggcctacg
     4921 gcgacgaggt ggagccctcg ccgctcgcgg ccgtc
//
LOCUS       DASWGG010000358         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2893423, whole genome shotgun sequence.
ACCESSION   DASWGG010000358 DASWGG010000000
VERSION     DASWGG010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2893423"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VF954_06505"
     CDS             complement(<1..526)
                     /locus_tag="VF954_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530769.1"
                     /translation="MFLVLGVGAGAVYAAIGLGLVLTWRASRVVNFAHGAMAMYATYV
                     FVELRVAGDLILPVVGIPARVHLAPSVAFFPALVVATAVAGALGLAVHGLVFRPLARA
                     GPLARVVASVGLMVALQAVVTLNVGSTDRPIAAVLPAAPLRLWGMVMPRDRLYLAAVV
                     VVAAGILTTVYRRSR"
     gene            602..1840
                     /locus_tag="VF954_06510"
     CDS             602..1840
                     /locus_tag="VF954_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9530770.1"
                     /translation="MARTSRTALLGVAALASLALAACGKSRSANPVASTPTSAPPTGP
                     PITVGLINTENAPLGSFPELGRGVQAAIRYVDEHLGGVAGRPLQVVPCITAGTPESSQ
                     ACANTLALKSPVAVIGGIDLSASASIPVLAKAGIPYVTGSPTVLEELDATGSYALTAG
                     QGGDILGEADYVINTFKPNRIAVVYPDLPGLLNDATQAASNVLRAKGIASKLVAVKPD
                     AADLAPALTAAMASNPDVLFGIFPAQGCARLMQARQALGVSVKTFYLGACAEQSVIQA
                     AGPAADQAYFASAYLPPTASVDPDVSSYDAAMRSYDGQDAKASELAQAGFADVMNIRF
                     LLGEAGTTPTASVLGNLLHTARDHRNFMAHAFTCDGQQAPPFNAVCNAEVRILQYHAG
                     TFSDLSGAWVTGADLLKLAG"
     gene            complement(1854..>2352)
                     /locus_tag="VF954_06515"
     CDS             complement(1854..>2352)
                     /locus_tag="VF954_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530771.1"
                     /translation="GKWDAVPAQVDGQVLAPGAGAWAAWYRARVDGALRILTAGRAEV
                     VWLGMAPEGIAGSAPATAALNDVLRAATEAWPGATFVDTAPVLGGPDGSYVQSLPGPG
                     RTITLRKPDGVHLCPDGVVRLAEATVAAVAAEPQLAAGAGWDAGAWRHDFRYYWAPGG
                     GCPGS"
BASE COUNT          357 a          905 c          811 g          279 t
ORIGIN      
        1 accggctacg gcggtagacg gtcgtgagga tcccggcggc gacgaccacc acggccgcga
       61 ggtacaggcg gtcccggggc atgaccatcc cccacagccg gaggggggcg gccgggagca
      121 cggcggcgat ggggcggtcg gtggagccga cgttcaaggt cacgacggcc tggagggcga
      181 ccatcagtcc gaccgacgcc accacccggg cgagaggccc ggcccgggcg aggggccgga
      241 agacgagacc gtgcaccgcc agtccgaggg cgccggccac ggccgtggcc acgaccagag
      301 ccgggaagaa agcgacgctc ggtgccagat ggacccgcgc cgggatcccg accacgggca
      361 ggatgaggtc gcctgcgacc cgcagctcca cgaacacgta cgtggcgtac atggccatcg
      421 ccccgtgggc gaagttgacg acgcgggacg ccctccacgt gagcaccagg cccagcccga
      481 tggcggcgta gacggccccg gcgccgacgc ccagcacgag aaacaccagg taggggcgga
      541 tgcccggacg ttagcccggc gaaattcgcc cgcgggtcat ggcggggacc aagatcgacg
      601 gatggcgcga acctcccgta ccgccctgct cggcgtcgcc gccctcgcgt cgctggcgct
      661 cgcggcgtgc ggcaagagcc gcagcgccaa cccggtcgcg tccacgccga cctcggcccc
      721 gcctaccggc ccgcccatca ccgtggggct catcaacacc gagaacgccc ccctcggctc
      781 cttcccggag ctgggccggg gggtccaggc cgccattcgc tacgtcgacg agcacctcgg
      841 cggtgtcgcc ggccgcccgc tccaggtcgt cccgtgcatc accgccggca cgccggagtc
      901 ctcgcaggcc tgcgccaaca ccctggctct caagtcgccc gtcgccgtca tcggggggat
      961 cgatctcagc gcctccgcgt ccatacccgt gctcgccaag gcgggcatcc cctatgtgac
     1021 cggctcaccg accgtcctgg aggagctgga cgcgaccggg tcctacgccc tcaccgccgg
     1081 gcagggcggc gacatcctgg gcgaggccga ctacgtgatc aacaccttca agcccaacag
     1141 gatcgccgtg gtgtacccag acctcccggg gctgctcaac gacgccaccc aggcggcgag
     1201 caacgtcctc agagccaagg gcatcgcctc gaagctggtg gcggtgaagc ccgatgcggc
     1261 cgacctggcg cccgcgctga cggcggcgat ggccagcaac ccggacgtgc tcttcgggat
     1321 cttcccggcc cagggctgcg cccgtctcat gcaggcccgg caggcgctcg gcgtcagcgt
     1381 caagacgttc tacctgggcg cgtgcgccga gcagtcggtc atccaggcgg ccgggccggc
     1441 cgccgaccag gcctacttcg ccagcgccta cctgcccccc accgcctcgg tcgatcccga
     1501 cgtcagcagt tacgacgcgg cgatgcgcag ctacgacggc caggatgcca aggcgtccga
     1561 gctggcccag gccgggttcg ccgacgtgat gaacatccgg ttcctgctcg gcgaggccgg
     1621 caccaccccg acggcgtccg tgctcggcaa cctgctgcac acggcgcgcg accaccgcaa
     1681 cttcatggcg cacgccttca cgtgcgacgg ccagcaggcc ccgcccttca acgctgtctg
     1741 caacgccgaa gtgcggatcc tgcaatatca cgccggcacc ttcagcgatc tctccggcgc
     1801 gtgggtcacc ggtgccgatc tcctcaagct ggcgggctga gcaccgccgg gtctcacgag
     1861 cccgggcaac cgccgccagg ggcccagtag tagcgaaagt cgtggcgcca tgcgccggcg
     1921 tcccaccccg ccccggcagc cagctggggc tcggccgcga cggccgcgac ggtggcctcg
     1981 gcgagacgaa cgacgccatc cgggcagagg tggaccccgt cgggcttgcg cagcgtgatg
     2041 gtccggccgg gcccgggcag actctggacg tagctgccgt cgggcccgcc gagcaccggg
     2101 gccgtgtcca cgaaggtggc gccgggccac gcttcggtcg cggcgcgcag cacgtcgttg
     2161 agcgcggccg tcgccggcgc cgatccggcg atgccctccg gggccatgcc cagccacacc
     2221 acctcggcac ggccggcggt caggatcctg agggcaccgt cgacccgggc ccggtaccac
     2281 gccgcccatg ctccagctcc cggggcgagg acctggccgt cgacctgtgc gggtacggcg
     2341 tcccatttcc cc
//
LOCUS       DASWGG010000359         3931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_476584, whole genome shotgun sequence.
ACCESSION   DASWGG010000359 DASWGG010000000
VERSION     DASWGG010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476584"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..621
                     /locus_tag="VF954_06520"
     CDS             184..621
                     /locus_tag="VF954_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 family protein"
                     /protein_id="HEX9530772.1"
                     /translation="MSDSPVHLSSGLPETVLPEPPAETDGRLRAALDHPPGTRRDAVA
                     DVAGANPRFLQAWAALGQLGRDDVEAYAYYRVGYHRGLDALRAAGWRGSGYVRWQHPS
                     NRGFLRCLDGLREMAGHLGEADEEERCAQFLHQLDPDWDRRGS"
     gene            652..1197
                     /locus_tag="VF954_06525"
     CDS             652..1197
                     /locus_tag="VF954_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9530773.1"
                     /translation="MAVKSDEPVGPFPPGADPDTYDKLRRRVLWKMPSGLFVVGSRSG
                     SRRNLMTLNWATQVAFEPKLLAISVEHPALTHQLIHEGGAFSLALIDREDRAIVRKFT
                     KPVEEDAGAQSLNGFAFRDGHTGVPILEQAAAWLECEVRQEVAVGGHTLFVGEVVDAG
                     FSAPEETAILRMEDTRMSYGG"
     gene            1261..1644
                     /locus_tag="VF954_06530"
     CDS             1261..1644
                     /locus_tag="VF954_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530774.1"
                     /translation="MGQNEELTMSRSTRRMRMAAVGLVAAGVLTLGVATEATAAPAAA
                     AAVGGCKHAAVREKGLEKLEARIDGRVAHLQAAEEKAASSGHARRAGRIAKRLEKLKD
                     RMARVQGRLAKLEARCGGAPPVAGS"
     gene            1740..2909
                     /locus_tag="VF954_06535"
     CDS             1740..2909
                     /locus_tag="VF954_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEX9530775.1"
                     /translation="MAAEWVDTDAGLADVVAALDGEEAYSVDTEFHRERTYYPRLALV
                     QVSWDSGAVLIDPLAVDLTPFASVLVGSGVAVAHAAEQDLEVLERACGVGPARLFDTQ
                     LAAGFLGTSTASLVSLVERMLGRQVLKGDRLTDWTRRPLSSEQRAYAASDVAHLLELA
                     EVITEQLTGRGRLVWAEQECELLLRRSRQPQDPATAWWRLKDSRSMRGAARLVAQAVA
                     SWREQRAAAQDLPPRFVLSDLALISIAHRPPRNREQLLAVRGLDGRQMRPAVADDLLE
                     VINDALSGDASAIRQPPSEDVDRSLRPAVALAAAWVAQRSADLDIDAALLATRADIAA
                     LLRGDRRSRLGAGWRAELIGEPLRRLAAGEAALAFDGAGSLLLEARSHLPVTLHD"
     gene            complement(3089..3316)
                     /locus_tag="VF954_06540"
     CDS             complement(3089..3316)
                     /locus_tag="VF954_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530776.1"
                     /translation="MAESFDANAMVGRFRERARAVRNRPLPPVEGPERKRFMAQAEID
                     FMDFAMLGDAEATLADGILTLTIDLRPKPGP"
     gene            3372..>3931
                     /locus_tag="VF954_06545"
     CDS             3372..>3931
                     /locus_tag="VF954_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530777.1"
                     /translation="MLASGRLRPVELAEAAVLGDLAALMVLAGAWLPAGNVLLALAVT
                     PFVTLMVRHRLRAVLVGGFSSAAVGLLIGGLGLAVDVAALALVGAVVGAALRRRLRLP
                     GGVALTVLTLWPPGALLTDGLLALLTRSRHLAIDQGRNSWQGLRRLLADGLTLAHVDA
                     SGPLRRLDGLVSSALRHWWLAIPAAEL"
BASE COUNT          489 a         1421 c         1455 g          566 t
ORIGIN      
        1 gggatcacga ggacgtgccc gggaaacagc ggccggcggt ccaggaaggc cacgacctcg
       61 gtctcgtcga gcaccgtggt cgccccctcc gctcccgagg caatggcgca gaacgcgcag
      121 tcccacccgg tgtccgacgc caccgctgaa gcctaccgag ccgacccgtg cgagcatgca
      181 ggtatgagcg acagccccgt gcacctgtcc tccggcctgc cggagacggt gctgcccgag
      241 ccacctgcgg agacggacgg acggctgcga gccgccctcg accatccgcc gggcacccgg
      301 cgggacgcgg tcgccgacgt ggctggcgcc aatccgcgtt ttctccaggc ctgggccgcc
      361 ctgggccagc tgggtcgcga cgacgtggag gcctacgcct actaccgggt gggctaccac
      421 cggggcctgg acgcgttgcg ggccgccggc tggcgcggct cgggctatgt gcgctggcag
      481 cacccgtcca accgcggctt cctgcgctgc ctggacggcc tgcgggagat ggccgggcac
      541 ctcggagagg ccgacgagga ggagcggtgc gcccagttcc tccaccagct cgaccccgac
      601 tgggaccggc ggggctccta gggcccagcc cgccggcggt agcttcggac gatggctgtc
      661 aagagcgacg aacccgtcgg ccccttcccg cccggcgccg accccgacac ctacgacaag
      721 cttcgccgcc gggtgctgtg gaagatgccc agcgggttgt tcgtggtcgg gagccgctcc
      781 ggttcccgac gcaatctcat gacgctcaac tgggccaccc aggtggcctt cgagcccaag
      841 cttctcgcca tctccgtgga gcacccggcc ctgacccacc agctcatcca cgagggcggg
      901 gccttcagcc tggccctgat cgaccgggag gaccgggcca tcgtgcggaa gttcaccaag
      961 ccggtcgagg aggacgctgg ggcccaaagc ctgaacggct tcgcgttccg cgacgggcac
     1021 accggcgtgc cgatcctcga gcaggccgcg gcctggctgg agtgcgaggt ccgccaggag
     1081 gtggccgtgg ggggccacac cttgttcgtg ggtgaggtgg tcgacgccgg cttctcggcg
     1141 cccgaggaga cggcgatcct gcggatggag gacacccgca tgagctacgg cggctgacag
     1201 ctcatcgcct gcccttggtc cgctcacaca tcggtcacaa ccctctccga acatctgggt
     1261 atgggccaga acgaggagct gacgatgtcc aggagcacgc gcaggatgag gatggcggcc
     1321 gtgggtttgg tggccgcagg tgtcctgacg ctgggcgtcg cgacagaggc caccgcggcg
     1381 ccggccgcgg ctgccgcggt ggggggctgc aagcacgccg cggtccggga gaaggggctg
     1441 gagaagctcg aggcccgcat cgacggccga gtggctcacc tgcaggcagc tgaggagaaa
     1501 gccgcctcca gcggccatgc gcgtcgcgcc gggcggatcg ccaaacggct ggagaagctg
     1561 aaggatcgca tggccagggt ccagggtcgg ctggcgaagc tcgaggccag gtgtggcggc
     1621 gcgcccccgg tggccggctc gtagcggttc ccgccgggag ctgcactacc gttgcaggta
     1681 tgcgcgaacg gagcggacac ccagctcgac ggcgccgggg ctgagctctg ccgaccgggg
     1741 tggccgccga gtgggtcgat accgacgccg gtttggccga cgtggtggcg gccctggacg
     1801 gcgaggaggc ctactccgtc gacaccgagt tccaccggga gcggacgtac tacccccggc
     1861 tggcgctggt ccaggtgtcg tgggactccg gcgcggtgct gatcgatccc ctcgcggtcg
     1921 atctgacgcc tttcgcgtcg gtcctggtcg gatcgggcgt cgccgtggcc catgccgccg
     1981 agcaggacct ggaggtcctc gaacgggcct gcggggtcgg gccggcccgc ctcttcgaca
     2041 cgcagttggc cgccggcttc ctcggcacgt ctaccgcgtc gctggtgtcg ctcgtcgaac
     2101 gcatgctcgg ccgccaggtg ctcaaaggcg accggctgac ggactggacc cggcgacccc
     2161 tgtcgtcgga gcagcgcgcc tacgcggcgt ccgacgtcgc ccatctgctc gagctggcgg
     2221 aggtgatcac cgagcagctc accggccggg gccggctggt gtgggccgag caggaatgcg
     2281 agctcctcct gcgccgcagc cgccagcccc aggatcccgc cacggcgtgg tggcggctca
     2341 aggacagccg ttcgatgcgc ggggcggccc ggctggtagc tcaggccgtc gccagctggc
     2401 gggagcagcg ggccgccgcc caggacctgc ccccccgctt cgtgctctcc gatctggccc
     2461 tcatctcgat cgcccaccgg ccgccgcgca accgtgagca gctcctggcc gtgcggggcc
     2521 tcgatggccg ccagatgaga ccggcggtcg ccgacgatct cctcgaggtc atcaacgacg
     2581 cattgagcgg tgacgcgtcc gccatccgcc aaccgccctc cgaggacgtc gaccggtcgc
     2641 tgcgcccggc cgtggccctg gccgccgcct gggtcgccca gcggtcggcc gacctcgaca
     2701 tcgacgcggc cctgctggcc actcgggccg acatcgccgc cctcctgcgc ggcgaccgcc
     2761 gctcccgcct gggcgccggc tggcgggcag agctgatcgg cgagcccctg cgccggctgg
     2821 ccgccggcga ggccgccctg gccttcgacg gtgcgggctc gctgttgctg gaggcgaggt
     2881 cccacctgcc cgtcacgctc cacgactgac gtccgtcctg gcgccggcga gggccctagg
     2941 ccgacggccc ggcgtggggt tggggcccca cgctcgggag gagggcgggg ttggggcccc
     3001 gcccgggcct atgagtagcg gcccggtgtg gggttggggc cccacgctcg ggaggagggc
     3061 ggggttgggg ccccgcccgg gcctatggct acgggcccgg cttgggccgc aggtcgatcg
     3121 tcaaggtgag gatgccgtcg gcgagggtgg cctcggcgtc gccgagcatg gcgaagtcca
     3181 tgaagtcgat ctcggcctgg gccatgaacc gcttgcgctc ggggccctcg acgggaggca
     3241 gcggacggtt gcggacggcg cgagctcgtt cccggaaccg acccaccatg gcgttggcat
     3301 caaagctttc ggccatgccg gggcaagcta gccgcggctg accggctgcc ggtccccggt
     3361 accttcgggt cgtgctcgca agcggccgac tgcgtcccgt tgagttggcc gaggcggccg
     3421 tgctcgggga cctcgccgcc ctgatggtcc tcgccggcgc ctggctgccg gcgggcaacg
     3481 ttctgttggc gctggccgtg acccccttcg tgaccctcat ggttcgccac cggctgcggg
     3541 ccgtgctggt cggggggttc tcctctgcag ccgtcggcct gctgatcggg gggttggggc
     3601 tggcggtcga cgtggccgcg ctggccctcg tgggcgccgt ggtcggcgcc gccctgcgcc
     3661 ggcggctccg gcttcccggc ggcgtggccc tgaccgtcct caccctctgg ccaccgggtg
     3721 cgctgctcac cgacggcctg ctcgccctgc tgacccggag ccggcacctg gcgatcgacc
     3781 agggccgcaa ctcctggcag ggactgcgcc gcctgctcgc cgacgggctc acgctggccc
     3841 acgtcgatgc atcggggccc ctccgtcgcc tcgacggcct ggtctcctct gcgcttcgcc
     3901 actggtggct ggccatcccc gcagcggagc t
//
LOCUS       DASWGG010000360         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_153830, whole genome shotgun sequence.
ACCESSION   DASWGG010000360 DASWGG010000000
VERSION     DASWGG010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153830"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VF954_06550"
     CDS             <1..638
                     /locus_tag="VF954_06550"
                     /inference="COORDINATES: protein motif:HMM:NF026049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530778.1"
                     /translation="NPYALSIVRGAAGVLIGRLSGFLAVVKSPSARTDNLIFAYGEVP
                     RSLELTGKVTRLMSGVVRTLEVHPERMWASLESGFSQATDLAEFVMQTCGVDYRTAYV
                     VVGAAVRRAAAEGLRGIDLTAEMIEEAAEARIGRRLGLAGTDLTEVLDPRAIVGTRRS
                     PGGAAPDVVRTMVADCRVAAGRIRACAERRQAGFDEAERALVKAAEEVAGR"
     gene            complement(641..1030)
                     /locus_tag="VF954_06555"
     CDS             complement(641..1030)
                     /locus_tag="VF954_06555"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9530779.1"
                     /translation="MSELELTAHWEGGYRMRTEVRGLEIRADEPPEYRGDNTGPMPTE
                     LFLASLASCFGMAVVHAGRKRGIDLPDVAVKVTGTYEGLRFARIRVEVISSHPREALE
                     PLLERAVSYCYVSNTLRHQPLLEFVIA"
     gene            1029..>1604
                     /locus_tag="VF954_06560"
     CDS             1029..>1604
                     /locus_tag="VF954_06560"
                     /inference="COORDINATES: protein motif:HMM:NF018275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEX9530780.1"
                     /translation="MTDHDSGSVVELPGRVAVVNVGLKLFGDAVRSQGRAVIDVDWRI
                     PAGGDAALVAALVRTYGTSSERVEEANAEVCRRLDSAAPVLSGLATARDVVPGMGERT
                     LLHPGPPVAWEDVCDPLRRSARAAVMAEGWAATPEAAEALIASGGVDLQPANEHATVG
                     PMATVLGPSAPVFVVENPQGGGQQGNRAFAGI"
BASE COUNT          196 a          576 c          615 g          217 t
ORIGIN      
        1 gcaaccccta cgccctctcg atcgtacggg gcgccgccgg ggtgctgatc gggcggctgt
       61 cgggcttcct cgccgtggtc aagtcgccga gcgcccgcac cgacaacctg atcttcgcct
      121 acggcgaggt cccccggtcc ctcgagctga ccgggaaggt gacccgcctg atgtcgggcg
      181 tcgtgaggac gctcgaggtc caccctgagc ggatgtgggc gtccctggag tccggcttct
      241 cccaggcgac cgatctcgcc gagttcgtca tgcagacctg cggcgtcgac taccgcaccg
      301 cctacgtggt ggtgggggcg gcggtccggc gcgcggcggc cgagggcctc cggggtatcg
      361 acctcaccgc cgagatgatc gaggaggcgg ccgaggcccg gatcgggcgg cggctgggcc
      421 tcgccggcac cgacctgacc gaggtgctcg acccccgggc gatcgtcggg acccgccgct
      481 cgcccggcgg tgccgcgccg gacgtcgtgc ggacgatggt ggccgactgc cgggtggcgg
      541 ccggccgcat ccgggcctgc gccgagcggc gccaggcggg cttcgacgag gcggagcgcg
      601 ccctcgtgaa ggctgccgag gaggtggcgg gccggtgacg ctaggcgatc acgaactcca
      661 gcaggggttg gtgccgcagc gtgttcgaga cgtagcagta gctgacggcc cgctccagca
      721 gcggctcgag tgcctcgcgg gggtgcgacg agatgacctc gacccggatc cgggcgaagc
      781 ggaggccctc gtaggtgccc gtgaccttca cggccacgtc cggcaggtcg atgccccgct
      841 tgcgcccggc gtgcacgacc gccatcccga agcacgaggc gagcgaggcc aggaacagct
      901 ccgttggcat cggccccgtg ttgtcgcccc ggtattcggg gggctcgtca gcccggatct
      961 cgagcccgcg cacctcggtc cgcatccggt agccgccctc ccagtgggcg gtgagctcga
     1021 gctcactcat gaccgaccac gatagcggct ccgtcgtgga gctccccggg cgggtcgccg
     1081 tcgtgaacgt cggcctcaag ctgttcggcg acgccgtgcg ctcccagggg cgggcggtca
     1141 tcgacgtcga ctggcgcatc cccgccgggg gcgacgcagc cctcgtcgcc gccctcgtca
     1201 ggacctacgg gacgtcctcg gagcgcgtcg aggaggccaa tgccgaggtc tgccggcgcc
     1261 tcgacagcgc cgcccccgtg ctgagcgggc tggcgacggc ccgcgacgtg gtgccgggga
     1321 tgggggagcg gacgctgctg caccccggcc ccccggttgc ctgggaggac gtctgcgacc
     1381 ccctgcggcg ctccgcccgg gccgcggtga tggcggaagg ctgggcggcg accccggagg
     1441 cggccgaggc gctgattgcc tcgggcggcg tggacctcca gccggccaac gagcacgcca
     1501 ccgtcgggcc gatggcgacg gtgctcgggc cctcggcgcc ggtgttcgtc gtcgagaacc
     1561 cgcagggtgg cggccagcag ggcaaccgtg cgttcgccgg gatc
//
LOCUS       DASWGG010000361         7450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2896696, whole genome shotgun sequence.
ACCESSION   DASWGG010000361 DASWGG010000000
VERSION     DASWGG010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2896696"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1385)
                     /locus_tag="VF954_06565"
     CDS             complement(156..1385)
                     /locus_tag="VF954_06565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9530781.1"
                     /translation="MGPLLRPQLVHPPRRRRPDPHPAGCVPPARGVLASRLSVLHVAQ
                     SSEYGLGRYLSELLSAERGAGWRVAMAGSAASSLRPRAAALGVSWWAWEARRGPGPSA
                     LAEAGRLRQVIARVDPDVVHLHCSKAGLVGRTMVRGRRPTLFQPHGWSFVASTGMTAE
                     AARRWEKMAARWAHSVVCCSRAEREEGIRAGVRARWRVVPNAVDLSRFPEPARGDRAA
                     ARHRLDLPAGPLAVCVGRLSDQKGQDRLLSVWPRIAAAVPGAALVLVGDGPDRVALAQ
                     RAPAGVRFVGWQGDVRSWLTAADVVVQPSRFEGMSIAVLEALACGRAVVATDVHGMSE
                     AITEGSLPRAGAVVPEDDDAALADAVVARLRSSQMVEAEGQAGRRRVAADHDLRHWAA
                     ELLDATERVAGLGRPAP"
     gene            complement(1286..2785)
                     /locus_tag="VF954_06570"
     CDS             complement(1286..2785)
                     /locus_tag="VF954_06570"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX9530782.1"
                     /translation="MKTLPLTTGFDASLAAVDKASALADPHLRAAAPRRRAGVARVAV
                     TVADAAALGVGLVVARRLALTVNPTLTHARGVLVGAPLLLALPLYLAVFARYRLYHAR
                     HVADRGQELTRLLHATAMGTVLVAVLASFLHRVLAWEWLVGAFGCGFAGVAAERELAR
                     LAFRAMRRRGHLLRRVAIVGVGEEAVATAQLLAGEPELGYRVVAFVGPDPILGISRSG
                     VPILSSPVKIAEQLRMVGAQGVIMATTDVDPTTSNRLVRRLTDEGVHVELTSSLTDID
                     ASRLSVRSLGRIPVLYVEAVSRGGWRALAKRSFDVFGATVIALLSLPFLPIVALAIKL
                     DSAGPVLFRQERVGRRGERFAVFKLRTMVQDAERHRAQLQLLNEADGPLFKVRHDPRV
                     TRVGRVLRNLSLDELPQIVNVLLGQMSLVGPRPALPSEVSEWSPELFERLKVRPGMTG
                     MWQVNGRSDSSFSAYQRWDLYYVHNWSILHDVGVLIRTLPAVFLQRGAC"
     gene            complement(2815..3099)
                     /locus_tag="VF954_06575"
     CDS             complement(2815..3099)
                     /locus_tag="VF954_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530783.1"
                     /translation="MKFSYLAAAGVLAASIALPGATALADTSDYTQTPPNVAGIQQTR
                     PLATPATPAVGGTSLPVTGGDLAELAAIGVVATGAGTVLVRRSRRPRDVQ"
     gene            complement(3243..5024)
                     /locus_tag="VF954_06580"
     CDS             complement(3243..5024)
                     /locus_tag="VF954_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEX9530784.1"
                     /translation="MSDAGVTVSDIPAAGPPRAHQSSRLPGWIWLVGTIALLWVLIAG
                     VEAALVAVHVHRGTAQVDEARRQLAPEDLKSGAALPALRRAGADFRAAHREASHPALL
                     PVRLLPVLGRQLRAVIALTQAGDRVTVVGADTIREADAALSAPHADGAARLALLHRIA
                     AVSMTAADRLRAVELGPSHGLLGPLASRRRRLGAGLERVVNAADRAAGATSAMADLLS
                     GRRYLVLAANNAEMRAGSGMFLSVGVLESSAGAFHLGPVRPSADFDLPGDGVALGGTL
                     AERWGWLHPNREWRNLGLSPQFDETGPLAARMWQAAQGQPIDGVLAVDVEALKTMLSV
                     TGPVATDRGEVSAGNVEPLLFHDQYVGVTPDPSSSVVRRDRLGAIASNALTALQGGGV
                     DLARLGHQLANASGGRHILAWAARPEIEAAWQRAGVGGTLEPNAAMVSVLNRGANKLD
                     QFIRVAPALTRHRVGSDTQITLSVTMRNDVPPNEPPYVAGVGEAGVPPGTYLGLLAID
                     LPAAAGDVQLVPAGPASVAGPDGASQVVATRIELGPGQVVTVALTFRVAGRHRRLRID
                     PSARVPPVLWTTGRSTFTDLSPHDLTW"
     gene            complement(5050..5709)
                     /locus_tag="VF954_06585"
     CDS             complement(5050..5709)
                     /locus_tag="VF954_06585"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX9530785.1"
                     /translation="MPGRDGSRRGRSAPCAQPEDGCQARVRTQAALLLAPGASADRSQ
                     PALVAIDGALASYGIPVERMDFPYKRAGRRAPDRPPVLILAVREAAAGLVSRAGVSPD
                     RLVLGGRSMGGRICSMAVGEGLPALALVLVSYPLHPPGRPDRLRVEHFPQLLPPCLFV
                     SGTRDAFATPEELHAATAVIPGPVTHAWIDGGDHGLGKRDLEVAEVVRRWVLELVAIG
                     A"
     gene            complement(5696..6394)
                     /locus_tag="VF954_06590"
     CDS             complement(5696..6394)
                     /locus_tag="VF954_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530786.1"
                     /translation="MLLAELEIRHSRAVAPTRRVALGELWLPTDPSPGFGGILIAGIV
                     ATFIAGLDEEMHDGLETLLTDVEGGRRVVQPRLRHRFQHDVVGLDRSRHTLIGHGEHV
                     RLELDDHGAAMPQILGALYAAGQLSYSARPSVWRLIRRATRWEGAAGDALVAYLTGDE
                     AAFRVDRLAVHGQAWALQVLGFPRDAAPERDEVLRRFRSLIRHAHPDHGGQTEGAGTL
                     IHQLTQAKRILAGT"
     gene            complement(6453..7088)
                     /locus_tag="VF954_06595"
     CDS             complement(6453..7088)
                     /locus_tag="VF954_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530787.1"
                     /translation="MSTVWCPRCGAEYVEGIVTCTDCGVPLVGTPPGSRTGDGGVGED
                     DTGPDREVVVYELDDWSDEERAELQVRLDAEGVTHSWDSATELVVAEGDEPRVDALLD
                     IVEDATALNEGDEDDSDADEANYEIISALFVAADRLTQGSPSNGALVDEFIDAAEAGD
                     GIPLPFGVDEDEWAQVLRITGAVRSDLAAASPRERINREATTLRDLLRGYV"
     gene            7168..>7450
                     /locus_tag="VF954_06600"
     CDS             7168..>7450
                     /locus_tag="VF954_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530788.1"
                     /translation="MGSDVPSEVAGDSASGDSASRGSVSGDASEVLGDSPPSASARAR
                     SAAEGRLALVRNAGPVRAHDSLEDRARALYLAAEQEVLAWLREADDERAV"
BASE COUNT         1181 a         2713 c         2598 g          958 t
ORIGIN      
        1 tgccggtcgc ggccatggcc gccaccccga ccggccaggg ctattggctg gcgacggtca
       61 ccggatcggt cgaggccttc ggcgacgcga ccgcgctcag cgcccagggt gtctccgtcg
      121 gcagcccggt cgtgagcctg gccccgtagc ggccgctacg gcgcggggcg gccgagtccc
      181 gccacccgct ccgtggcgtc cagcagctcg gcggcccagt ggcggagatc gtggtcggca
      241 gccaccctcc ggcgacccgc ctgtccctcc gcctcgacca tctgtgacga tcgcaggcgg
      301 gcgacaacgg catccgccag agcggcgtcg tcatcctcgg gcacgaccgc gccagcccga
      361 gggagcgatc cctcggtgat cgcctcggac atcccgtgca cgtccgtggc caccaccgcc
      421 cgcccgcacg ccagggcctc gagaacggcg atcgacatgc cctcgaagcg ggatggttgg
      481 acgaccacgt cagccgccgt caaccaagac cggacgtctc cctgccagcc gacgaaccgg
      541 acgcccgcgg gtgcccgttg cgccagcgcc acgcggtccg ggccgtcgcc caccagcacg
      601 agggcggcgc ccggcacggc ggccgcgatc cggggccaga cggacagcag ccggtcctgg
      661 cccttctgat ccgacaaccg gcccacgcac acggccagcg gaccggccgg caggtccagc
      721 cggtgccgtg ccgcggcgcg gtcaccgcgg gccggctccg ggaacctcga caggtcgacg
      781 gcgttgggca ccacccgcca ccgagcccga acaccggccc gaatcccctc ctcgcgctcc
      841 gctcgactgc agcacaccac gctgtgcgcc caccgggctg ccatcttctc ccaacgacgg
      901 gcggcctcgg ccgtcatccc ggtcgaggcc acgaacgacc aaccgtgggg ctggaagagc
      961 gtcgggcgcc gcccccggac catcgtccgg ccaaccagcc cggccttgga acagtgcagg
     1021 tggaccacgt cgggatcgac tcgagcgatg acctggcgca atcgcccggc ttcagccaga
     1081 gccgagggcc ccggcccgcg cctcgcctcc cacgcccacc agctcacccc aagagccgca
     1141 gcccgaggcc gcagcgaact ggccgccgag ccagccatcg ccacacgcca accggccccg
     1201 cgctccgccg agaggagctc ggacagatat cgacccagcc cgtactccga cgactgggcc
     1261 acatgaagga cggacagccg gctggctagc acgccccgcg ctggaggaac acagccggca
     1321 gggtgcggat caggacgccg acgtcgtgga ggatggacca gttgtggacg tagtagaggt
     1381 cccaccgctg gtaggcgctg aagctcgaat cggaacggcc gttcacctgc cacatgccgg
     1441 tcatgccggg ccggaccttc agccgctcga agagctcggg cgaccattcg gacacctcgg
     1501 aagggagggc gggtctcgga ccgaccagac tcatctggcc aagcaacacg ttgacgatct
     1561 gagggagctc gtcgagcgag aggttgcgca ggacccgccc gacccgggtc acccggggat
     1621 cgtgacggac cttgaagagc ggcccgtccg cctcgttcag caactgcagc tgggcgcggt
     1681 gccgctcggc gtcctgcacc atggtacgga gcttgaacac agcgaagcgc tcgccccgtc
     1741 ggcccacccg ctcctggcgg aacagcacgg gcccggcgga gtccagcttg atggccaagg
     1801 cgacgatcgg gagaaagggc aggctgagca gggcgatgac agtggccccg aaaacatcga
     1861 agctccgctt ggccagggcc ctccaaccgc cccgtgacac ggcttcgacg tacaggacgg
     1921 gtatccgccc aagcgagcgc acggacaggc gagacgcgtc gatgtcggtg agggacgacg
     1981 tcagctcgac gtgaacgccc tcgtcggtca accgccggac cagccggttg gacgtagtcg
     2041 ggtcgacgtc ggtggtggcc atgatcacgc cttgtgcgcc gaccatgcgc agctgctcgg
     2101 cgatcttgac cggactgctg aggatcggca cacccgagcg cgagataccc aggatcggat
     2161 cgggaccgac gaaggcgacg acccggtatc ccagctcggg ctcgcccgcc aggagctggg
     2221 cggtcgcgac ggcctcctca cccacaccca cgatggcgac gcggcgcagg aggtggccgc
     2281 gccggcgcat ggccctaaag gccagtctcg ccagctcccg ctctgccgcg acgcccgcga
     2341 acccgcaacc gaacgccccc accaaccact cccaggccaa gacgcggtgc aggaaggagg
     2401 cgaggaccgc caccagcacg gtgcccatcg ccgtggcgtg cagcaatcgc gtgagctcct
     2461 ggcctcggtc ggcaacatgg cgggcgtggt agaggcggta gcgggcgaag accgcgagat
     2521 acagcggcaa cgccaagagg aggggggcac ctacgagcac gccccgagcg tgagtcaagg
     2581 tggggttcac ggtcagcgcc aggcgccgcg cgaccacgag gccgacgccg agggctgcgg
     2641 cgtccgcgac cgtgaccgcc acccgggcga cgccggcgcg acgacgcggg gcggcggcgc
     2701 gcaggtgtgg atcagccagc gccgacgcct tgtcgaccgc ggccagggac gcgtcgaacc
     2761 ccgtggtgag agggagggtt ttcattggat gggcaggaat agccgtggga ctggctactg
     2821 cacgtcgcgg ggtcggcggc tacgccgcac caacaccgtg ccggcccctg tcgccaccac
     2881 gccgatggcg gcgagctcgg cgaggtcgcc gcccgtcacc ggcaggctcg tgccaccaac
     2941 cgcgggggtg gccggggtgg ccaggggtct ggtctgctgg atgcccgcga cgttgggcgg
     3001 ggtctgggtg taatcgctcg tgtcggctag agccgtggca cctgggagcg caatgcttgc
     3061 ggccaggacg cccgcggctg cgagatagct gaatttcatt acgtctctct ccgtgaccgg
     3121 ccggagggcc gttcgtcgtc aaatgccact gtacgtgata actagtccgc cgccaagtcc
     3181 caatttgcgg tggaatcgcc tccttcacca ctcgatggac tagctataga ccttccccgt
     3241 ggtcaccacg tcaaatcgtg gggcgaaagg tccgtgaagg tcgaccgtcc ggtggtccac
     3301 aggacgggag gaactcgggc agaagggtcg atgcgcagcc tacggtgccg cccggcgacc
     3361 cggaacgtca acgccacggt caccacctgc ccgggcccga gctcgatccg cgtggccacc
     3421 acctggctgg cgccgtcggg cccagccacc gacgccggac cggccggcac gagctggacg
     3481 tcgcccgcag ccgcgggaag gtcgattgcc agcagaccca gatacgtccc aggcgggaca
     3541 cccgcctcgc cgactccggc cacatagggc ggctcgttcg gcggcacgtc gttgcgcatg
     3601 gtcacgctca gggttatctg ggtatcggag cccacgcggt gccgggtcaa cgcgggagcg
     3661 acccgaatga actggtcgag cttgttggcg ccccggttga gaaccgacac catcgccgcg
     3721 tttggctcca acgtgccacc gaccccggcc ctctgccacg ccgcctcgat ctcgggccgg
     3781 gcggcccacg ccaagatgtg acggccgccc gacgcgttcg ccagctgatg cccgagccgg
     3841 gccaggtcga caccaccgcc ctggagggcg gtgagggcat tgctggcgat cgccccgagc
     3901 cggtcccggc gaacgaccga agacgacgga tcaggagtga caccgacgta ctggtcgtgg
     3961 aacaggagcg gctcgacgtt gccggctgag acctcaccac ggtccgtggc caccggtccg
     4021 gtgacgctga gcatcgtctt gagcgcctcg acgtcaacgg ccagaactcc gtcgatcggc
     4081 tggccctggg ccgcctgcca catgcgagcc gccagtgggc cggtctcgtc gaactggggg
     4141 ctcagcccga ggttgcgcca ctcgcggttg gggtggagcc aaccccagcg ttcggccagc
     4201 gtgccaccca gcgccacgcc gtcgcccggc aggtcgaaat ccgccgaagg ccggaccggg
     4261 ccgagatgaa acgcccccgc gctgctctcg aggacaccga ccgacaggaa catccccgag
     4321 ccggcgcgca tctcggcatt gttcgcggcc agcaccagat accggcggcc ggagaggagg
     4381 tcggccatgg ccgaggtggc gccggcggcg cggtcggcgg cgttgacgac ccgctcgaga
     4441 ccggcgccga ggcgacgccg tcgactcgcc aacgggccga gcaacccgtg ggacgggccc
     4501 agctcgaccg ctcgaagccg gtccgcagcc gtcatggaga cggcggcgat ccggtgcaac
     4561 aacgcgagac gcgcggcacc gtcggcgtgg ggtgcggaca gggccgcgtc ggcttcgcgg
     4621 atggtgtcag cgccgaccac ggtcacccgg tcgccggcct gcgtaagggc gatcacggcc
     4681 cgtagctggc ggccgagcac cggcagcagg cgaaccggga ggagcgccgg atggctggcc
     4741 tcccggtggg cggcccggaa gtcggcgccc gctcgccgga gcgcgggcag ggctgcgccg
     4801 ctcttcaggt cttcaggggc caattggcgg cgggcctcat cgacctgcgc ggtcccgcgg
     4861 tgcacatgga cggccaccag cgccgcctca accccggcga tcagcaccca cagcagcgca
     4921 atcgtgccca ccagccagat ccaccccggc agccggctgc tctggtgagc tcggggcggg
     4981 ccagcggccg ggatgtcgct gacggtcacc cccgcgtcag acatgtctca cgcgagcgtt
     5041 gccaccagct caagcaccta tcgccaccag ttcaaggacc catcggcgga ccacctcggc
     5101 cacctcgagg tcccgcttgc ccaggccgtg atcgccgcca tcgatccagg catgggtcac
     5161 cggccccggg atgacggccg tggcggcgtg cagctcctcc ggtgtcgcga acgcgtcccg
     5221 cgtgcccgac acgaacaggc acggtggcag gagttggggg aaatgctcga cccgcaggcg
     5281 gtccggccgt ccgggcggat ggagcggata gctcaccagc acgagggcca gggcagggag
     5341 tccttcgccg acagccatcg agcagatgcg cccgcccatc gaccggcccc cgagcacgag
     5401 ccggtcgggt gacacgccgg cacgcgacac caggccggcg gccgcctccc tgacggccag
     5461 gatcagcacg ggagggcggt caggcgcccg cctgccggcc ctcttgtagg gaaagtccat
     5521 ccgctcgacc gggatcccgt aggaggcgag ggctccgtcg atggcgacca gggcaggttg
     5581 actgcgatcc gcgctcgctc cgggggcaag cagcagcgcg gcctgagtcc ggacccgcgc
     5641 ctggcaccca tcttccggct gggcgcaggg cgcgctccgc cctcggcggg agccgtcacg
     5701 tcccggcaag gatccgcttc gcctgggtca gctgatggat gagcgtgccc gccccttccg
     5761 tctgcccacc gtggtccgga tgggcgtggc ggatcagcga acggaagcgg cgcaacacct
     5821 cgtcgcgctc gggtgccgcg tcccgtggga aacccaggac ctgaagcgcc cacgcctggc
     5881 cgtggaccgc caggcggtcg actctgaagg cagcctcgtc acccgtgaga taggccacga
     5941 gggcatcgcc ggcggcgccc tcccagcggg tcgcccgccg aatgagccgc cacaccgacg
     6001 gccgggcgct gtatgacaac tggccggcgg catacagcgc cccgaggatc tgcggcatcg
     6061 cagcgccgtg atcgtcgagc tcgaggcgaa cgtgctcccc gtgcccgatc agggtgtggc
     6121 ggctccggtc gaggccgacc acgtcgtgct ggaaccgatg gcgcagccgc ggctgcacca
     6181 ccctacggcc tccttcgaca tcggtcagca gcgtctcgag gccatcatgc atctcttcgt
     6241 ccaggcctgc gatgaaggtc gccacgatgc ccgcgatgag gatcccgccg aacccgggtg
     6301 aggggtccgt cggcagccac agctcgccga gagcgacacg ccgggtcggg gccacggcgc
     6361 gggagtgacg aatctccagc tcggcgagga gcacgggtcg aaggtagacg acatcggcgc
     6421 tctcttccta ccgccgcggg gaccgcgacg cgtcagacat agcctcgcag aaggtcgcga
     6481 agcgtggtgg cctcacggtt gatgcgctct ctcggcgaag cggcggccag gtcggaccgc
     6541 accgcaccgg tgatgcgcag gacctgggcc cactcgtcct cgtcgacgcc gaatggcaga
     6601 ggtatgccgt cgccggcctc ggcggcatcg atgaactcgt cgacgagcgc gccattgctc
     6661 ggcgagccct gggtcagccg gtcggctgcc acgaacaacg ccgagatgat ctcgtagttg
     6721 gcctcgtccg catcgctgtc gtcctcgtca ccctcattga gcgcggtggc gtcctcgacg
     6781 atgtcgagga gggcgtcgac cctgggctcg tctccctcgg cgacaaccag ctccgtcgcg
     6841 ctgtcccagc tgtgggtgac gccctccgca tccagacgga cctggagctc ggcccgttcc
     6901 tcgtcgctcc aatcgtccag ctcgtacacg accacctcgc ggtcgggtcc ggtgtcgtcc
     6961 tcgccgacgc cgccgtcgcc ggtgcgcgag ccgggcggcg tgccgactag cggcacaccg
     7021 caatcggtgc atgtgacgat gccctcgacg tactcggctc cgcagcgggg acaccacacg
     7081 gtgctcatgg ccacccacgc tatgacctgg ccgacggcct cgccgagagg cgtccgatac
     7141 ccacggcggc gaggtagcct tcgacgggtg ggttcggacg tcccttccga ggtcgccggt
     7201 gattcggcct ccggtgattc ggcctcccgt ggttcggtct ccggtgatgc gtccgaggtg
     7261 ctcggcgact cgcccccgtc cgcgtccgcg cgcgcccgat cggcagcgga gggccgcctc
     7321 gccctggtgc gcaacgccgg tccggtgcga gcgcacgata gcctcgaaga ccgggcccgg
     7381 gcgctgtacc tggctgccga gcaagaggtg ctggcctggt tgcgagaagc cgacgacgaa
     7441 cgggccgtcg
//
LOCUS       DASWGG010000362         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_156189, whole genome shotgun sequence.
ACCESSION   DASWGG010000362 DASWGG010000000
VERSION     DASWGG010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156189"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VF954_06605"
     CDS             complement(<1..675)
                     /locus_tag="VF954_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX9530789.1"
                     /translation="MATDYYELLGVTRHATDDEIKKAYRRLARELHPDLNPGDKSSED
                     RFKQVTVAYETLCDPERRRRYDMFGPEGVQAPTDPFSTMAGAGIGDLFDAFFGGASPF
                     GGGRQRGYGGPPRGSDAELALDLSFEEAVFGVAKDVSLRANVTCGTCGGSGARPGTTP
                     TRCGNCRGSGEVRRVRQSILGQMVTATPCHRCGGTGEEVASPCADCHGDGRRTEERTY
                     SVDVPGG"
     gene            complement(726..>1596)
                     /locus_tag="VF954_06610"
     CDS             complement(726..>1596)
                     /locus_tag="VF954_06610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9530790.1"
                     /translation="LQDAGYEVGEAATGLDGIQAALATRPDVVLLDLVLPDLPGMEVL
                     RRMRSHDLLTSTAVVIVSERVRVADVAAVLQAGAHDYLRKPVETGELIARVTAAGRAK
                     RLHDEVLGHRAAPEEARRTDHVTGLPNRLHLDEYLQMGMALARRYGTPLAALALDVDH
                     LKRINDGGRQRGDAVLALIGGALRDALRQEDLVGRWGGDEFLALLLGDGASDVVAPAH
                     RILSSVRQLPRVHGMPPVTVSIGAALYVPPEDGRSLVRRADAALYAAQVSGRDTMVVA
                     APPGETGETGETG"
BASE COUNT          196 a          591 c          595 g          214 t
ORIGIN      
        1 gccgccgggc acgtccaccg agtaggtccg ctcctcggtg cgccggccgt cgccgtggca
       61 gtccgcgcat ggtgacgcca cctcctcgcc tgtgcccccg caccggtggc acggcgtggc
      121 cgtgaccatc tggcccagga tggactgccg caccctgcga acctcccccg agccgcggca
      181 gttgccgcac cgggtgggcg tcgtgccggg ccgggcgccg gagcccccgc aggtgccgca
      241 cgtcacgttg gcgcgcagcg acacgtcctt ggcgaccccg aagaccgcct cctcgaagga
      301 cagatccagc gccagctcgg cgtcggagcc gcgcggcggg ccaccgtagc cccgctggcg
      361 cccgccgccg aacgggctgg ccccgccgaa gaaggcgtcg aagaggtcgc cgatgccggc
      421 gccggccatg gtggagaaag ggtccgtcgg agcctgcacg ccctcgggcc cgaacatgtc
      481 gtagcgccgc cggcgctcgg ggtcgcagag ggtctcgtag gccaccgtga cctgcttgaa
      541 ccggtcctcg gacgacttgt cgccagggtt caggtcgggg tgcagctcgc gcgccaggcg
      601 ccggtaggcc ttcttgatct cgtcgtcggt cgcgtggcgc gtgacgccga ggagctcgta
      661 gtaatccgtg gccatggctg gcggcagttt accggcccat gggtcgccct ccgtgggcga
      721 cccgcctacc ccgtctcgcc cgtctcgccc gtctcgccgg gtggcgcggc cacgaccatg
      781 gtgtcccgac cggacacctg ggcggcgtag agggcggcgt ccgcccggcg caccaggctg
      841 cgcccgtcct cgggcggcac gtagagcgcg gctccgatgc tgaccgtgac gggcggcatg
      901 ccgtgcacgc ggggaagctg gcgcaccgac gacaggatcc ggtgcgccgg ggcgacgacg
      961 tcggaggcgc cgtcgccgag cagcagggcc aggaactcgt cacctcccca gcgccccacc
     1021 aggtcctcct ggcgcagggc gtcgcgcagg gccccaccta tcaacgccag gaccgcgtcg
     1081 cctcgttgcc ggccgccgtc gttgatgcgt ttgaggtggt cgacgtcgag cgccagggcg
     1141 gcgaggggcg tgccgtagcg gcgggccagg gccatgccca tctggaggta ctcgtcgagg
     1201 tggaggcggt tggggaggcc ggtgacgtga tcggtgcgcc gtgcctcctc cggcgcggcg
     1261 cggtggccca ggacctcgtc gtgcaaccgc ttggcccgcc cggccgccgt cacccgggcg
     1321 atgagctccc cggtctcgac cggcttgcgc aggtagtcgt gggcgcccgc ttgcagaacc
     1381 gccgccacgt cggccacccg gacccgctcg ctcacgatca cgacggcggt gctggtcagc
     1441 aggtcgtggg agcgcatccg ccggaggacc tccatgccgg gcaggtcggg caggacgagg
     1501 tcgaggagca ccacgtcggg gcgggtcgcc agggcggcct ggatgccgtc caggccggtg
     1561 gccgcctccc cgacctcgta acccgcgtcc tgcaag
//
LOCUS       DASWGG010000363         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_481033, whole genome shotgun sequence.
ACCESSION   DASWGG010000363 DASWGG010000000
VERSION     DASWGG010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481033"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VF954_06615"
     CDS             complement(<1..357)
                     /locus_tag="VF954_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9530791.1"
                     /translation="MAVRVVIAEDEAIIRLDLKEMLENEGYQVVGETGRGDEAVALVR
                     ELSPDLAVLDIKMPGMDGLSAAREISAERLSAVLILTAFSQRNLIEEARDAGALAYLV
                     KPFQKAELIPAIEVALG"
     gene            complement(385..678)
                     /locus_tag="VF954_06620"
     CDS             complement(385..678)
                     /locus_tag="VF954_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530792.1"
                     /translation="MSRSLIERRLVEVNRRLRRAREELSVVDEQLGALTEAAEETRIR
                     ALVSETPLADHDHRDAQRHADAMARSRAAAMASIAELERAQDDLLDRLVIESR"
     gene            complement(713..795)
                     /locus_tag="VF954_06625"
     tRNA            complement(713..795)
                     /locus_tag="VF954_06625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(760..762),aa:Leu,seq:caa)
     gene            1111..2346
                     /gene="fabD"
                     /locus_tag="VF954_06630"
     CDS             1111..2346
                     /gene="fabD"
                     /locus_tag="VF954_06630"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX9530793.1"
                     /translation="MLAFTFPGQGSQRPAMGAAWQDHPSWELVEDASDAADRDIAHLL
                     LTAGADELRQTHNAQLATFTLSLVALDAVERLGLEPACCAGHSLGEYTALVASGAISF
                     EEGVRLVAERGEAMQHAADERPGSMSALLGLDDDAADAACRRAEADVWVANYNAPGQV
                     VIAGQKEDVDNASAVAKELGAKRAMPLPVGGAFHTPLMEPARNRLRKALTATTFRDPE
                     VPVTANVDARPHCDGGEWPSLLSAQLCNPVRWRQTVAQLAGFGVTQVVEVGPGGVLTG
                     LARRCAPDLQAVSVAAPQDLDDLVAILAGTSFREYADTHQGEHLYISERLVLSPAAGL
                     FTPIVVTTEAAAVEATPTVVDVGAVDVAIDVGALIGNVGGAEVRSPFAGWLMGLLAVD
                     GERVSVGQPVAWLRATGVD"
     gene            2399..>2599
                     /locus_tag="VF954_06635"
     CDS             2399..>2599
                     /locus_tag="VF954_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEX9530794.1"
                     /translation="MTNHDFEARGMDTNDEWIVERTGIRERRWGGSTSVMAVDAGRQA
                     LAHAGLVPEDVDLLVLATTTPDQ"
BASE COUNT          362 a          900 c          952 g          385 t
ORIGIN      
        1 gccgagcgcc acttcgatgg cggggatgag ctcggccttt tggaagggct tgaccaggta
       61 ggcgagggcc ccggcgtccc gggcctcctc gatgaggttg cgctggctga acgcggtcag
      121 gatcagcacc gccgagaggc gctcggccga gatctcgcgg gcggccgaca gcccgtccat
      181 cccgggcatc ttgatgtcca gcacggcgag gtcgggcgac agctcccgga cgagcgccac
      241 ggcctcgtcg ccgcgcccgg tctcgccgac cacctggtag ccctcgttct cgagcatctc
      301 cttgagatcg agccggatga tggcctcatc ttcggctatg acgacgcgga cggccaacgg
      361 ggtcgctcct tggggttgag tgagctagcg ggattcgatc acgagccggt cgagcaggtc
      421 atcctgggcg cgctcgagct cggcgatgga ggccatggcg gcggcccggc tgcgcgccat
      481 ggcgtcggcg tggcgctggg cgtcgcggtg gtcgtggtcg gccagcggag tctccgatac
      541 cagggcccgg atccgggtct cctcggcggc ctccgtcagg gccccgagct gctcatctac
      601 gaccgagagc tcctcgcggg cgcgccggag gcggcggttg acctccacca gccggcgctc
      661 gatgagtgac cgcgacatgg ccggagtcta gaggccgcgg cccgcgagct cgtgcggcgg
      721 gtgggattcg aacccacatg ccctttcgag cagcggcttt tgaggccgcc atgtaaaccg
      781 ttccatcacc gccgcagcag tggatgtcaa ggtaccggat ggcactggtc aacagcgggt
      841 ttcgagtccc tgaccggtta ccgcggagta aggcgacaac cgccttgtcg gtcggtaagg
      901 tcatcagatg cctcgtcgcc ggcgccggct gatcgtcctg gcggcctcgc tgggcgcgct
      961 gttcgcctac cgacagcggc gggtcgcgga caacgagcgg aagttcgggg ttcgttgaaa
     1021 ccttgttctt cgacaacgag tctgagcact gtggccggtc cggcatcctg ccgggccgtg
     1081 gcgaggtagc gcggagcagg gagggacgca ttgctggctt tcacattccc cgggcagggt
     1141 tcacagcggc cagcgatggg cgccgcatgg caggaccatc cgtcgtggga actggtggag
     1201 gatgcctcgg acgcggccga ccgggacatc gcccacctcc tcctcaccgc gggcgccgac
     1261 gagctcaggc agacccacaa cgcccagctc gccaccttca ccctctccct cgtcgcgctc
     1321 gacgccgtcg agcgtctcgg cctcgaaccc gcctgctgcg ccggccacag cctgggtgag
     1381 tacaccgccc tggttgcgag cggggccatc tcgttcgagg agggcgtccg gctggtggcc
     1441 gagcgtgggg aggccatgca gcacgcggcc gacgagcggc ccggctccat gtcggcgttg
     1501 ctcgggctcg acgacgacgc cgccgacgcc gcctgccgcc gggccgaggc cgacgtgtgg
     1561 gtggccaact acaacgcgcc gggccaggtg gtcatcgccg gccagaagga ggacgtggac
     1621 aacgccagcg ccgtcgccaa ggagctggga gccaagcgcg ccatgcccct gccggtcggc
     1681 ggcgccttcc acacgccgct catggagccg gcgcgcaacc ggctgcgcaa ggccctgacc
     1741 gccacgacgt tcagggaccc ggaggtgccg gtcaccgcca atgtcgacgc ccgcccgcac
     1801 tgcgacggcg gcgagtggcc gagcctgctc tccgcccagc tgtgcaaccc cgtgcggtgg
     1861 cgccagacgg tcgcgcagct cgccggcttc ggcgtgaccc aggtggtcga ggtcggcccg
     1921 ggcggcgtgc tgaccggact ggcccggcgc tgcgcccctg atctccaggc cgtgtcggtc
     1981 gcggccccgc aggacctcga cgacctggtc gccatcctgg ccggcacctc cttccgggag
     2041 tacgccgaca cccaccaggg cgagcacctg tacatctcgg agcgcctggt gctgagcccg
     2101 gcggccgggc tgttcacgcc gatcgtggtg acgaccgagg ccgcggccgt ggaggcgacc
     2161 cccaccgtgg tcgacgtcgg ggccgtcgac gtggcgatcg acgtcggggc gctcatcggc
     2221 aacgtcggtg gggccgaggt tcggtccccg ttcgcgggct ggctcatggg actgctggcc
     2281 gtcgacggcg aacgcgtctc cgtaggccag cctgtggcgt ggctccgggc cacgggggtg
     2341 gactgaattg cgaggagcgg cgctgaccgg ctggggtgct gcgctgcccg accaggtggt
     2401 gaccaaccac gacttcgagg cgcgagggat ggacaccaac gacgagtgga tcgtcgagcg
     2461 cacaggcatc cgcgagcgac gatggggcgg ctcgacgtct gtgatggccg tcgacgccgg
     2521 ccgccaggcc ttggcgcacg ccgggctggt tcccgaggac gtggacctgc tggtgctggc
     2581 cacgacgacc cccgaccag
//
LOCUS       DASWGG010000364         5277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2097087, whole genome shotgun sequence.
ACCESSION   DASWGG010000364 DASWGG010000000
VERSION     DASWGG010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097087"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VF954_06640"
     CDS             complement(<1..386)
                     /locus_tag="VF954_06640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9530795.1"
                     /translation="MAARVEEAAPPGARDPIDVQLVPMRRRHLRAVLRIESQVYPRPW
                     SLSLFMSELALRSTRAYYVARVDGVVCGYSGLMFTGEDAHVTTIAVDPAWHRHRIGTR
                     LMLNNARVALGRGCRHLTLEVRLGNVG"
     gene            complement(388..1107)
                     /gene="tsaB"
                     /locus_tag="VF954_06645"
     CDS             complement(388..1107)
                     /gene="tsaB"
                     /locus_tag="VF954_06645"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX9530796.1"
                     /translation="MIILGIDTATDQVGVALGGVEGVLAEFRVARGRRHAETLAPAVD
                     FVCRQAGIELSDVSVVAVDVGPGLFTGLRVGLAAAKAIASALRTPMIGLPSLDLLAFP
                     LRHTARTVVPVVDARRSEVFTTTYRTVPGGLQRLAPYRLCSPTDLLAELQATDEEHVL
                     AGDGALRYAEVFSGDSRIELATVGYAYPSAAALVELSQPRALREDFVQPWEVQPLYLR
                     RPDAEINWDRRHPSGLLARGT"
     gene            complement(1112..1597)
                     /gene="tsaE"
                     /locus_tag="VF954_06650"
     CDS             complement(1112..1597)
                     /gene="tsaE"
                     /locus_tag="VF954_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX9530797.1"
                     /translation="MISALTKSPDDTRELAAAVSTLARPGDVVLLAGELGSGKTVFAQ
                     GFGRGLGVEGPVTSPTFTLVRTYRGRLPLVHADVYRLDRLQEIVDLGLAELLDDGAVA
                     LVEWGDVAAATLPADYLEVRFAFGGGDDERRLDLRVVGPAWSPRGRALQDAVGRWCQG
                     A"
     gene            complement(1594..2238)
                     /locus_tag="VF954_06655"
     CDS             complement(1594..2238)
                     /locus_tag="VF954_06655"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9530798.1"
                     /translation="MPTLAELERDVVDCRRCPLAEGRTQVVFGVGHPQAPLMFVGEAP
                     GAEEDRRGQPFVGRSGQLLDRLMAEELGMSRSQAYIANVVMCRPPGNRDPRPVEIEAC
                     RPNLEAKLDLIRPAVIMTLGNFAARTLLGATEGITRLRGRSYPFRGGVLVPTYHPSLA
                     AAQRRRRVLAEMRADFIRAKEALVAAGALGPAGVVEPGVGEPAGAGPAPGPGPR"
     gene            2355..4541
                     /locus_tag="VF954_06660"
     CDS             2355..4541
                     /locus_tag="VF954_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX9530799.1"
                     /translation="MSLDTLTQSAGAPTTSHGDREQGAAPQDPDGGGGRAGRAAPSRL
                     ALRLALLAGVGGAALAAGAAVAVAAATLVGSPMSTKARPITLPGLDSRSTVYAADGSV
                     LAVLHADQDRVPVTLAQVPPVVQNAVVDTEDAQFWKEGAVSARSAARALITNLNAGGI
                     RQGGSTITQQLVKNTLLSSKQDLHRKIQEAVLAMRLEKQLTKGQILERYLNTVYFGNG
                     AYGVQAAAEHYFGIPVGQLQPDQAALLAGLIRNPVGYDPIRDPGDARARRSVVLGLMV
                     QHGHLAADQQARAEGAALPTAIVNPPAPEDYFTAAVKDELLGDCRLGATPQERFHAVF
                     GGGLAIHTTLDPGLQQRAADTVRSGLPDTGGRLTAALVSIEPASGAVRALVGGPDFHH
                     SQFNLALDGIGRQPGSSFKPFTLVTALEKGYSPNDTIDGTQPCSIPNPGGTPDPWRPG
                     NFEGEGGGVMTLTDATAHSINCAYAQLALMVGSRSIADVAHAMGITARLNPVPSMTLG
                     TEEVTPLQMASAYSTLAADGVHHTAHLVDHVDGPDGKTLFRTTEPGRRAVPAQISREA
                     VQVMRQVVIRGTGRNANLADRPVAGKTGTAENFHDAWFVGYTPQLSTAVWMGDPAGEV
                     PMTDVGGISVVGGSFPARIWRAFMEGALAGRPALDFPEPDPGQVPAGQFLADGVGRGP
                     NGGSTTGVTTWCWASCGKAGPTPANPPAGAPPPAPPAPAPGKPPKH"
     gene            complement(4538..>5277)
                     /locus_tag="VF954_06665"
     CDS             complement(4538..>5277)
                     /locus_tag="VF954_06665"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX9530800.1"
                     /translation="GEVRGGAPGTREMDLLQPERRVEHLDAVVLTGGSAFGLAACDGV
                     ARWCEERGLGFPTSAGPVPIVVGAVLYDLAVGDPSVRPGPAEGYAACVAATSGPVRVG
                     RVGAGTGATVGKWRGREQARPGGIGTATERDGAVVVAALVAVNAWGDATGGGQRADLS
                     PTSSASPFENTTIGVIATNATVSKLGCLLVAQSGHDGLARALEPVHATVDGDALVAAS
                     VGGVEADLDRVRILAARAVEAAVRAVV"
BASE COUNT          727 a         2014 c         1932 g          604 t
ORIGIN      
        1 ccgacgttgc cgagccgtac ctcgagggtg agatggcggc agccccgtcc cagggcgacc
       61 cgggcgttgt tgagcatgag gcgggtgccg atcctgtggc ggtgccaggc agggtctacg
      121 gcgatggtcg ttacgtgggc gtcctcgccg gtgaacatca gcccggagta gccgcacacc
      181 accccgtcga ccctggccac gtagtaggcg cgcgtcgagc gcagtgcgag ctcgctcatg
      241 aacaggctca gcgaccacgg ccgggggtac acctgggact cgatgcgcag cacggcccgc
      301 aggtggcggc ggcgcatggg cacgagctgc acgtcgatcg ggtcgcgggc gcccgggggg
      361 gcggcttcct cgacgcgggc ggccacctca ggtcccccgc gccagcaggc cggaggggtg
      421 gcggcggtcc cagttgatct cggcgtcggg ccggcgcagg tacaagggct ggacctccca
      481 cggctgaacg aagtcctcgc gaagggcgcg aggctgggac agctcgacca gcgcggcggc
      541 cgacgggtag gcgtagccca ccgtggccag ctcgatgcgg ctgtccccgg agaagacctc
      601 ggcgtagcgc agggcgccgt cgcccgccag gacgtgctcc tcgtcggtgg cctggagctc
      661 ggccagcagg tccgtcggcg agcacagccg gtagggcgcg agccgctgga gcccgcccgg
      721 caccgtgcgg taggtagtgg tgaacacctc ggagcgccgg gcgtcgacga cgggcacgac
      781 cgtgcgggcc gtgtggcgca gcgggaaggc caggaggtcc aggctgggca ggccgatcat
      841 cggggtgcgc agggccgagg cgatggcctt ggccgcggcc agccccacgc gcagccccgt
      901 gaacaggccc gggcccacgt ccacggccac cacgctgaca tccgacagct cgatgcccgc
      961 ctgccggcac acgaaatcca cggccggcgc cagggtctcg gcgtggcgcc tgcccctggc
     1021 gacgcggaac tcggcgagca cgccctcgac ccctccgagc gccaccccga cctgatcggt
     1081 ggccgtgtcg atgccgagga tgatcacgct ctcacgcccc ctggcaccat cgaccgaccg
     1141 cgtcctggag ggcccgcccg cggggcgacc acgccggccc caccacccgg aggtccaggc
     1201 gccgctcgtc gtcgcccccc ccgaaggcga agcggacctc gaggtagtcg gccgggaggg
     1261 tcgcggcggc cacgtcgccc cactccacca gcgccaccgc cccgtcgtcg agcagctcgg
     1321 ccaggccgag gtcgacgatc tcctggaggc ggtcgaggcg gtagacgtcg gcgtgcacca
     1381 gcggcaaccg gccccggtag gtgcgcacca gggtgaaggt cgggctggtc accggcccct
     1441 cgacgccgag cccgcgcccg aagccctggg cgaagaccgt cttgcccgag cccagctcgc
     1501 cggccagcag gacgacgtcg ccgggcctgg ccagggtcga gaccgcggcg gccagctccc
     1561 gggtgtcgtc cggcgacttg gtgagggcgg agatcaccgc ggcccgggcc cgggtgcggg
     1621 ccccgcgccg gcgggctccc cgacgccagg ctccacgacg ccggcaggcc ccagggcgcc
     1681 cgcggccacc aacgcctcct tggcccggat gaagtcggcg cgcatctcgg ccagcacccg
     1741 tcggcggcgc tgcgcagcgg cgagggacgg gtggtacgtg gggaccagca cgccgccgcg
     1801 gaaggggtag ctgcggcccc gcagcctggt gatgccctcg gtcgcgccga gcagggtccg
     1861 ggccgcgaag ttgccgagcg tcatgatcac cgccgggcgg atcaggtcga gcttggcctc
     1921 gaggttgggc cggcaggcct cgatctcaac gggtcgggga tcgcggttgc cgggcgggcg
     1981 gcacatgacc acgttggcta tgtaggcctg cgaacgggac atgcccagct cctcggccat
     2041 cagccggtcc aggagctgtc ccgaccgacc gacgaacggc tggccccgcc tgtcctcctc
     2101 ggcgcccggc gcctcgccca cgaacatgag cggggcctgc gggtggccca ccccgaacac
     2161 gacctgggtc cggccctcgg ccagcgggca gcgccggcaa tccacgacgt cgcgttcgag
     2221 ctccgccaac gtgggcacgc tccgatgtta cgtcggcgtg ttacgtcggc gcccggcccg
     2281 ccggctgggg ttcaacccgg acggccgtgg gtagtcccct tgcgggcgcc gcccaccccg
     2341 gaggactgcc gccgatgtcg ctcgacacac tcacgcaaag cgccggggcc cccacgacca
     2401 gccacggcga ccgggagcag ggcgccgcgc cccaggaccc ggacgggggc ggcgggcggg
     2461 cggggcgggc ggcgccgtca cggctggccc tgcgtctggc cctcctggcg ggcgtgggcg
     2521 gcgccgcgtt ggcggccggc gccgcagtgg cggtcgccgc cgccaccctg gtgggctcgc
     2581 ccatgtcgac gaaggcccgg cccatcaccc tgcccggcct ggactctcga tcgacggtct
     2641 atgcggccga cggctccgtg ctggccgtgc tgcacgccga ccaggaccgt gtgccagtga
     2701 cgctcgccca ggtcccgccg gtcgtgcaga acgcggtggt cgacaccgag gacgcccagt
     2761 tctggaagga gggcgcggtc agcgcccggt ccgccgcccg ggccctgatc accaacctga
     2821 acgccggcgg catccggcag ggcggctcga ccatcaccca gcagctggtg aagaacaccc
     2881 tgctcagctc caagcaggac ctgcaccgca agatccagga ggcggtcctg gccatgcggc
     2941 tggagaagca gctgaccaag ggccagatcc tggagcggta cctcaacacg gtctacttcg
     3001 gcaacggcgc ctacggcgtg caggccgccg cggagcacta cttcgggatc cccgtgggcc
     3061 agctccagcc cgaccaagcc gccctgctgg ccgggctgat ccgcaacccg gtgggctacg
     3121 acccgatccg cgaccccggc gacgcccggg cccggcgcag cgtggtgctc ggcctgatgg
     3181 tccagcacgg ccacctggcg gccgatcagc aggccagggc ggagggcgcc gcgttgccca
     3241 cggcgatcgt caacccgccc gcgcccgagg actacttcac cgccgcggtc aaagacgagc
     3301 tcctcggcga ctgtcggttg ggggccacgc cgcaggagcg gttccatgcg gtgttcggcg
     3361 gcggcctggc gatccacacg accctcgacc ccgggctcca gcaacgggcg gccgacacgg
     3421 tccgcagcgg cctgcccgac accgggggcc ggctgaccgc cgcactcgtg tcgatcgagc
     3481 ccgccagcgg tgccgtccgg gccctggtcg gcgggcccga cttccaccat tcgcagttca
     3541 acctggccct ggacgggatc gggcggcagc ccgggtcgtc gttcaagccg ttcacgctgg
     3601 tgaccgccct ggagaagggg tactcgccca acgacacgat cgacggcacc cagccctgct
     3661 ccatcccgaa cccggggggc acgcccgacc cgtggcggcc cggcaacttc gagggcgagg
     3721 gcggcggggt catgaccctg accgatgcca ccgcgcactc catcaactgc gcgtacgccc
     3781 agctcgccct gatggtcggg tcgcgcagca tcgcggacgt ggcccacgcc atgggcatca
     3841 ccgcccgtct caacccggtg ccctcgatga cgctcgggac cgaggaggtc acccccctcc
     3901 agatggcctc ggcctactcc accttggcgg ccgacggcgt ccaccacacc gcccacctcg
     3961 tcgaccacgt cgacgggccc gacggcaaga cgctgttccg cacgaccgaa ccgggacggc
     4021 gggccgtccc cgcgcagatc tcgcgggagg cggtgcaggt catgcggcag gtcgtcatcc
     4081 gcggcacggg ccgcaacgcc aacctggcgg accggccggt cgccggcaag acgggcacgg
     4141 ccgagaactt ccacgacgcc tggttcgtgg gctacacgcc ccagctgtcg acggcggtgt
     4201 ggatgggcga cccggccggc gaggtgccca tgaccgacgt gggcggcatc tcggtcgtcg
     4261 ggggcagctt cccggccagg atctggagag cgttcatgga gggcgcgctg gccggccggc
     4321 ccgccctcga cttcccggaa ccggacccgg gccaggtccc ggcgggccag ttcctggccg
     4381 acggtgtggg gcgcggcccc aacggcggaa gcaccacggg cgtgaccacg tggtgctggg
     4441 cgtcgtgcgg caaggccggg cccacgccgg ccaaccctcc ggcgggcgcg cctcccccgg
     4501 cgccgcccgc gcccgctccg ggcaagccgc ccaagcacta gacgacggcg cgcacggcgg
     4561 cctcgaccgc ccgggcggcc aggatgcgaa cccggtccag gtccgcctcc acgcccccca
     4621 cggaggcggc gaccagtgcg tcgccgtcga cggtggcgtg gacgggctcg agcgccctgg
     4681 ccagcccgtc gtggccggac tgggcgacga gcaggcagcc gagcttggaa acggtcgcgt
     4741 tggtggcgat gacgccgatg gtggtgttct cgaaagggga ggcggacgag gtgggagaaa
     4801 ggtcggcccg ttgccctcca cccgtcgcgt cgccccaggc gttgacggcc accagggcgg
     4861 ccaccaccac cgccccgtcg cgttcggtgg ccgtgccgat gccgccgggc cgggcctgct
     4921 cccggccccg ccacttgccc acggtcgcac cggtacctgc cccgacgcga ccgacccgga
     4981 cggggccgga ggtggccgcc acgcaggccg cgtagccctc ggccggcccc ggccggacgg
     5041 acgggtcgcc cacggccagg tcgtacagca cggcgcccac cacgatcggc accggcccgg
     5101 cggaggtcgg gaagcccagg ccccgctcct cgcaccagcg ggccacgccg tcgcaggccg
     5161 ccagcccgaa ggccgagcct ccggtcagca cgaccgcatc caggtgctcg acgcgccgct
     5221 cgggctggag gaggtccatc tcgcgtgtgc ccggcgcccc gccccgcact tcgcccg
//
LOCUS       DASWGG010000365         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2109964, whole genome shotgun sequence.
ACCESSION   DASWGG010000365 DASWGG010000000
VERSION     DASWGG010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2109964"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1913
                     /locus_tag="VF954_06670"
     CDS             <1..>1913
                     /locus_tag="VF954_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX9530801.1"
                     /translation="AGTIELADEDGAALTVSASDLTVCFQTAGKAIPLVFVSACTTGQ
                     SATGLAYELHRLGVARVLAMQAPVSDRYATELAAAFYAQLSTRPAPRAGVALAAGRRE
                     AETARSRDPARVNRPEWATPTLLLAGDDEPLIDPDLELVQLATPAMSAGGMVPMLAMG
                     ELIGRRRELRTTLRALRGDHRFTDEHGEVAGVMLTGIGGVGKSSIAGRAMARLTEGGW
                     LCSATTGTWSLETVCAQLGADLSRARQVWARQERAALADLPADDRYRIAWLATLLGQH
                     RVLLVFDNFEDNLVVGGGAFLDEPAAEALQGLLDSCASGKVLVTSRHPLPVVIGLHEV
                     PVGPLSPAETRRLFLRLPGLQRLAPDDVALVAATVGGHPRALELLDALLRKGASLRRV
                     GPKLRDLARREGIDTSAGRDLAAALSDTVRLAARDIVLDELLEVLDDTERQVLLQVAV
                     SGLPVPADGLATGLEDAGVIPAALEAAAARLGDLSLVVVFDGGLWVHRWTAEAIRRRE
                     PAEDYRRRCERAGRMRLRGIESGPADIAEGIEATENFLAAQLFDEAAGIAMGVAEFLS
                     GVSTLHLLSFSRRVRQALPTSHVRYKNVADYEAKALLALGFTTAALERYGELVHDLEA
                     LAEAERDRADYQR"
BASE COUNT          266 a          668 c          703 g          276 t
ORIGIN      
        1 ggcgggaacg atcgagctcg cagatgagga cggagcggcg ctgaccgtct cggcgtccga
       61 cctcacggtg tgtttccaga cggcgggcaa ggccatacca ctcgtcttcg tctccgcctg
      121 caccaccggc cagagcgcga ccgggctggc ctacgagctg caccgccttg gcgtggctcg
      181 ggtgctggcc atgcaggccc cggtcagcga ccgctacgcc accgagctgg ccgcagcctt
      241 ctatgcccaa ctctccaccc ggccggctcc ccgggccggg gtcgccctgg ccgccggccg
      301 gcgggaggcc gagacggctc ggagccggga ccccgcccgg gtcaaccgcc ccgagtgggc
      361 aacgccgacg ctgctgctcg ccggggacga cgagcccctg atcgacccgg acctggaact
      421 ggtccagctc gccacaccgg cgatgtccgc cgggggaatg gtccccatgc tcgccatggg
      481 ggagctgatc ggacggcgcc gtgagcttcg cacgacgctg cgggcgctgc gaggcgacca
      541 caggttcacc gacgagcacg gcgaggtcgc cggcgtgatg ctgaccggca tcgggggagt
      601 gggcaaatcc agcatcgccg ggcgggcgat ggcccgcctg accgaggggg gatggttgtg
      661 ctcggccacc acgggcacct ggagcctgga gacggtgtgt gcccagctgg gcgccgactt
      721 aagccgggcg cgccaggtct gggcacgcca ggaacgggcc gccctggcag acctgccggc
      781 cgacgaccgc tatcggatcg cgtggctcgc caccctgctc ggccagcacc gggttctgct
      841 cgtcttcgac aacttcgagg acaacctcgt cgtcggcggg ggggccttcc tcgacgagcc
      901 cgcggcggaa gccctccagg gcctgctcga ctcctgcgcc agcggcaagg tgctcgtcac
      961 ctcccgccac cccctaccgg tggtgatcgg gctgcacgag gtgccggtcg gcccgctcag
     1021 cccggcggag acccgcaggc tgttcctgcg cctgccgggc ctgcagagac tcgcccccga
     1081 cgatgtggcc ctggtggccg caacggtggg aggacatcct cgggccctcg agctgctcga
     1141 cgctctgctg cgcaaggggg cgagcctccg gcgggtgggg cccaagctgc gggacctcgc
     1201 ccggcgcgag ggcatcgaca cttcggcggg ccgtgacctg gcggctgccc tgagcgacac
     1261 ggtgcgcctc gccgcccggg acatcgtgct cgacgagctg ttggaggtcc tagacgacac
     1321 cgagcggcag gtgctgctgc aggtggcggt gtcgggccta ccggtacccg cagacgggct
     1381 ggcgacgggg ctcgaggatg cgggggtgat accggcggcc ctggaggccg ccgccgcccg
     1441 tctgggtgac ctttccctgg tcgtggtgtt cgacggtggt ctctgggtgc accgctggac
     1501 ggcggaggcg attcgccgtc gtgagcccgc cgaggactat cggaggcggt gcgagcgggc
     1561 tggccgaatg aggctgaggg gcatcgaatc cggccctgca gatattgccg agggcatcga
     1621 agcgacggag aacttcctcg ccgcccaatt gttcgatgag gcggctggga tagcgatggg
     1681 ggtggccgag ttcctcagcg gagtctccac cctgcatctg ctgagcttct cccgtcgggt
     1741 acgccaggcc ttgcccacct cacacgtccg gtacaagaac gtcgcggact acgaggccaa
     1801 ggcactcctg gccctgggtt tcaccacggc ggccctggaa cgctatgggg agctggtcca
     1861 cgatcttgag gccctggccg aggccgagcg cgaccgcgcc gactaccagc ggg
//
LOCUS       DASWGG010000366         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_506245, whole genome shotgun sequence.
ACCESSION   DASWGG010000366 DASWGG010000000
VERSION     DASWGG010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506245"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..>2030)
                     /locus_tag="VF954_06675"
     CDS             complement(75..>2030)
                     /locus_tag="VF954_06675"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX9530802.1"
                     /translation="AKGAWESVRRHFRDFGVDADTAPITAVGIGDMSGDVFGNGLLCS
                     RALRLVAAFDHRHVFLDPDPDPATAAAERERLFNLPRSSWADYDPAVISAGGGVFPRT
                     AKLIPLSAEVRAALGTEAEALRPADLVKAVLRAPVDLLWNGGIGTYVKASTETHGDVG
                     DKANDAVRIDATELRARVVGEGGNLGFTQRGRIEFALAGGRINTDAIDNSGGVDCSDH
                     EVNIKILLDTVVADGEMTVRQRDRLLAEMTGEVAAMVLRDNYDQTGALATARAQAAPM
                     VDVHARYLRKLESEGGLDRAIEFLPTDEVLAQRRSAGTGLTSPEFAVLLAYTKLDVAG
                     HLLASEACEDPWFHRELAAYFPAPLRDERFVSAMVRHPLRREIIATRVTNLLVDRAGT
                     SFIHRLAEETGASVPELARAHAAAWEIFRLEELWTAVEALDNVVPAATQVDMMLPVRR
                     LAERATRWLVRHGPKPLDVAAAVGAFGSGAARLAGLLPALLSAADREAAEARAARWVD
                     AGAGKDLAARVTALDGLAPALDVLQVAGDDETVGDVAAVYFALGEQLELDWLRDRIAA
                     LPRDDRWQALARSALGDDYARERAALTAEALRGGGVEEWMARHRPVIERFLLVIDDIR
                     AGATPDLATLSVAMREARALSSPSSGR"
BASE COUNT          261 a          725 c          786 g          258 t
ORIGIN      
        1 gggagtttca cctgcctccc ccaaggcatg acgggcggtc gtggcagatg ccggagccgg
       61 agtcggcgcc ggcctcagcg tccgcttgac gggctgctga gggcccgggc ctcccgcatg
      121 gccaccgaca gggtggccag atcaggggtt gccccggccc ggatgtcgtc gatcacgagg
      181 aggaaccgct cgatgaccgg ccggtgccgg gccatccact cctccacccc gccaccgcgg
      241 agcgcctcgg cggtgagggc ggcccgctcc cgggcgtagt cgtcgccgag ggccgaccgg
      301 gccagcgcct gccaccggtc gtcgcggggc agggcggcga tccgatcgcg cagccagtcg
      361 agctcgagtt gctccccgag ggcgaagtag acggcggcca cgtcgccgac ggtctcgtcg
      421 tcccctgcca cctggagcac gtcgagcgcc ggggcgagac cgtcgagggc cgtcacccgg
      481 gcggcgaggt ccttgccggc gccggcgtcg acccagcggg cggcccgggc ctctgcggcc
      541 tcccggtcag cggccgacag cagcgccggc agcagaccgg cgaggcgggc cgccccggac
      601 ccgaaggcgc ccaccgccgc cgcgacgtcg aggggcttcg gtccgtggcg caccagccac
      661 cgggtggccc gttcggccag ccggcggacg ggcagcatca tgtcgacctg ggtggcggcg
      721 ggcacgacgt tgtcgagggc ctcgaccgcc gtccacagct cctcgagtcg gaagatctcc
      781 caggcggcag cgtgggcccg ggccagctcc gggacggagg cgccggtctc ctcggccagg
      841 cggtggatga agctggtgcc ggcccggtcg acgagcaggt tggtgacccg ggtggcgatg
      901 atctcccgcc gcagcgggtg gcggaccatg gccgagacga agcgctcgtc gcgcagtggc
      961 gccgggaagt aggcggccag ctcccggtgg aaccaggggt cctcgcaggc ctcggaggcc
     1021 agcaggtgac cggccacgtc cagcttggtg taggccagca gcacggcgaa ctcggggctg
     1081 gtgaggcccg tcccggccga gcgccgctgg gccaggacct cgtcggtggg gaggaactcg
     1141 atggcccggt cgaggccgcc ctcgctctcc agcttgcgga ggtagcgggc gtggacgtcg
     1201 accatcggcg ccgcctgggc ccgggcggtg gccagcgccc cggtctggtc gtagttgtcc
     1261 cgcagcacca tggccgccac ctcgccggtc atctcggcca ggagccggtc gcgctggcgg
     1321 acggtcatct cgccgtcggc cacgacggtg tcgagcagga tcttgatgtt gacctcgtgg
     1381 tcggagcagt cgaccccacc ggagttgtcg atggcgtcgg tgttgatacg cccgccggcc
     1441 agggcgaact cgatccggcc ccgctgcgtg aagccgaggt tgccgccctc ccccaccacc
     1501 cgggcccgca actcggtggc gtcgatgcgc accgcgtcgt tggccttgtc gccgacgtcg
     1561 ccgtgcgtct cggtcgacgc cttgacgtag gtgccgatgc cgccgttcca gagcaggtcg
     1621 accggcgccc gcagcacggc cttgacgaga tcggcgggcc ggagggcctc ggcctcggtg
     1681 ccgagggccg cccgcacctc ggcggacagc gggatcaact tggcggtgcg ggggaacaca
     1741 ccgccgccgg cggagatgac ggccgggtcg tagtcggccc acgacgaccg gggcaggttg
     1801 aacagccgct cccgctcggc ggcggcggtc gccgggtcgg ggtcggggtc gaggaacacg
     1861 tggcggtggt cgaaggccgc caccagccgg agggcccggg agcacagcag cccgttgccg
     1921 aacacgtcgc ccgacatgtc gccgatgccg acggcggtga tgggggcggt gtcggcgtcg
     1981 acgccgaagt cccggaagtg gcgccgtacc gactcccagg cgcccttggc
//
LOCUS       DASWGG010000367         1753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2420594, whole genome shotgun sequence.
ACCESSION   DASWGG010000367 DASWGG010000000
VERSION     DASWGG010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2420594"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VF954_06680"
     CDS             complement(<1..1122)
                     /locus_tag="VF954_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX9530803.1"
                     /translation="MALSEDEVRAAIGRLRDPVLRLPLADTGMLHHVRLRRGGRVEAT
                     VALPTVDYPEPEVLAEEVKAAARNLAGVQQVDVVLMPMGEGGQVALAAHLRTSASGPA
                     TPPTSGPTGGPGGAAPPPIGHEQGRPNPFNRPGARTRLLGISSGKGGVGKSSVTVNTA
                     LALARKGHEVAILDADVYGPSVPGMMGIRRPPMVLDEFMIPPVAFGVRCISIGFFVAD
                     DQPVIWRGPMLHKALEQFLVDVFWGELDYLLIDMPPGTGDVALSMAQYLPRSEVFVVT
                     TPQAAARRVAQRTAYMARKVHLPLAGVIENMSWFTGDDGKRYEIFGAGGGQELADLLS
                     VPLLGRIPLVPQLRQGGDTGVPIMVSAPESEAAAAFQSIA"
     gene            1195..>1753
                     /locus_tag="VF954_06685"
     CDS             1195..>1753
                     /locus_tag="VF954_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9530804.1"
                     /translation="MDAVADRPLSDGEFSAAVVAVTSAFGDPTRRAIYLSARDSGPGL
                     TAAEVAEAFDLHPNVARHHLDKLAAGGYLDVHIERGGGGAGRPSKRYRASAKGVPVDL
                     PARQADLLVTLLGRALALLPARQAEAMAEQVGEDYGRALAEQMAPSLGAPGLGAGSDG
                     QRSVRSALHAVADALTAHGFAAHAED"
BASE COUNT          242 a          624 c          652 g          235 t
ORIGIN      
        1 ggcgatcgac tgaaaggctg ccgcggcctc gctctcgggg gccgagacca tgatcggcac
       61 gccggtgtcg ccgccctggc ggagctgggg gaccagaggg atccggccga ggaggggcac
      121 gctcagaagg tcggctagct cctggcctcc gcccgctccg aagatctcgt accgcttgcc
      181 gtcgtcgccg gtgaaccacg acatgttctc gatcaccccg gccagcggca ggtggacctt
      241 cctggccatg taggcggtgc gctgcgccac ccggcgggcc gccgcctgcg gggtcgtgac
      301 cacgaagacc tcggaccggg gcaggtactg ggccatggag agggccacgt cgcccgtgcc
      361 agggggcatg tcgatcagga gatagtccag ctccccccag aacacgtcca cgaggaactg
      421 ctccagggcc ttgtgcagca tcgggccccg ccagatcacg ggctggtcgt cggcgacgaa
      481 gaacccgatg gagatgcatc tcacgccgaa ggcaaccggg gggatcatga actcgtcgag
      541 gaccatgggg gggcggcgga tgcccatcat ccccggtacc gacgggccat agacgtcggc
      601 gtcgaggatg gccacctcgt ggcctttccg ggccaacgcc agcgcggtgt tgaccgtcac
      661 tgaggacttg cccacgccgc ccttgccgga ggagatgccg agcaggcggg tgcgggcgcc
      721 gggccggttg aacgggttgg gccggccctg ctcgtggccg atgggcggcg gcgcggcgcc
      781 accagggccc ccggtcgggc ccgacgtcgg gggcgtggca gggccgctcg ccgaggtccg
      841 gaggtgggcg gccaacgcca cctggccgcc ctcgcccatc ggcatcagca cgacgtcgac
      901 ctgctggacc cccgccaggt tccgggcggc ggccttgacc tcctcggcca acacctccgg
      961 ctcggggtag tcgacggtgg ggagcgcgac ggtcgcctcc acccggccgc cccgccggag
     1021 cctgacgtgg tgcagcatcc ccgtgtcggc gagcggaagc cgcagcacgg gatcacgaag
     1081 gcgcccgatg gcggcgcgga cttcgtcttc ggagagggcc atggagactc ctgcgctcct
     1141 gcggggatgc ttcgaggggc gaatttcccc tatcgccgta gtagaatagc gatcgtggat
     1201 gccgtggccg accggccgct gtctgacggc gagttctcgg ccgccgtcgt cgccgtgacc
     1261 tccgcgttcg gcgacccgac ccgccgggcc atctacctgt cggcccggga cagcgggccg
     1321 gggctcaccg cggccgaggt ggcggaggcc ttcgacctgc acccgaacgt cgcccgccac
     1381 catctcgaca agctggctgc gggtgggtac ctcgacgtgc acatcgagcg aggcggcggc
     1441 ggggccgggc ggccgtccaa gcggtaccgg gcctcggcca agggcgtacc ggtggatctc
     1501 cccgcccgcc aagccgacct gctggtcacc ttgctgggcc gggccctggc gctgctgcct
     1561 gcgcgccagg ccgaggccat ggccgagcag gtgggcgaag actacggacg cgccctggcc
     1621 gagcagatgg cgccgagcct gggcgcaccg ggcctgggcg ccgggtccga cggccagcgc
     1681 tccgtgcgtt ccgccctcca cgcggttgcc gatgcgctga cggcccacgg gttcgcggcc
     1741 catgccgagg acc
//
LOCUS       DASWGG010000368         1849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2119321, whole genome shotgun sequence.
ACCESSION   DASWGG010000368 DASWGG010000000
VERSION     DASWGG010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119321"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VF954_06690"
     CDS             complement(<1..767)
                     /locus_tag="VF954_06690"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX9530805.1"
                     /translation="MTLTGHGTSDAIVSRLAVDLDRAESGVGFVYDALSTLVTLYGLG
                     EAVLVIDDPVIGRQAFRAGRHPLIPGGGGQESRAGRMAGSVLSTAAGLHCEPEVVDRE
                     VAASVTALCRVAVQLDVRRHDASHDPLTGLYNRRSFDVGLERSASRSLRYGWPFALAL
                     LDVDRFKALNDRLGHHGGDQVLRVVGHELRRSLRAGDTAARVGGDEFGLILSNDRLEQ
                     VAPLLERLEHAVQAAVGVELGFSAGVATAPGDGTDAV"
     gene            1344..>1849
                     /locus_tag="VF954_06695"
     CDS             1344..>1849
                     /locus_tag="VF954_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530806.1"
                     /translation="MDVDVDPMEHELCRIPEVTAARIVVDGLGEVAEVHILATPTKQP
                     KQVVRDVQSVAVASFGVELDRRIISVVQLEDRAQAGDAGGNCSTRAVQSQPMAPEPAR
                     RAAIGDLEPGAASRARVVVDGVAYARNGYRCSVEVSLRLGEERAVGQAEGAASSGSAS
                     RLAAAATLS"
BASE COUNT          282 a          680 c          634 g          253 t
ORIGIN      
        1 tcgacggcgt cggtgccgtc accgggcgcc gtggccaccc ccgccgagaa cccgagctcc
       61 acccccaccg cggcctgcac ggcatgctcg agccgctcca gcagcggggc gacctgctcc
      121 aggcggtcgt tgctcaggat caggccgaac tcgtcgcctc ccacccgggc cgccgtgtcg
      181 cccgcacgca gcgagcgccg cagctcatgg cccaccaccc gtagcacctg gtccccgccg
      241 tggtggccca gccgatcgtt cagggccttg aaccggtcga catccaggag ggcgagggcg
      301 aagggccacc cgtaccgcaa gctgcgcgac gccgagcgct cgaggccgac gtcgaacgac
      361 cgccggttgt agagaccggt cagcggatcg tgggacgcgt cgtggcgcct cacgtcgagc
      421 tgcaccgcca cgcggcacag cgccgtgacc gaggccgcga cctcccggtc gaccacctcc
      481 ggctcgcagt gcaaccccgc cgccgtgctc agcacggagc cggccatgcg accggcccgg
      541 ctctcctggc cgcctccgcc ggggatgagc gggtggcggc cggcccggaa agcctggcgt
      601 ccgatgaccg gatcgtcgat gaccaggacc gcctcaccca gcccgtacag ggtcaccagg
      661 gtcgagaggg cgtcgtagac gaagcccacg ccggactcgg cgcggtccag atcgacggcc
      721 aggcgggaca cgatggcatc gctcgtaccg tgacccgtca acgtcacctc ccgctcccgt
      781 ctccgcgccc tggcgggcac ctgcgacacg ccagccgcgg caccacgccg actgctcacc
      841 cggcgtagcg atctcggagg caaagttcga cgcggcgtcg ccgaagtcct gccggggcgc
      901 ggcgcgagcg tcgagtgagg cctcccgcct ccccaggtct ccgtcacatc agaaggtcaa
      961 ctacgggaat agccagcgcc gcctgcgagc aggacggcga ccacgctcgc ggcaactgcg
     1021 gcgagccgaa cataggcctt cttcatgaag gacaccccct ttcggcttgg ctgatgacgc
     1081 ccgtatccgc tagcacggga cagatccgct ccgcttcgct tcggggaatg cggcgaaagg
     1141 cctcgctcgg ccctcgcacc atctgttgtc aatgtgcagt gtccgacgtg gatgcctgaa
     1201 cgccattcac catggcagcc cacgatcacg gtagcaaccc cgtcgggaga attttcgagc
     1261 cagctcgacc gggatcacca acaccccgag ccggtggtga ccatcccggt ccggcggtgg
     1321 ggctcctaac tactctgagt ggtgtggacg tcgacgtcga cccgatggag cacgagctgt
     1381 gccgcatccc ggaggtgacg gccgcccgga tcgtggtcga cggccttggt gaggtggcag
     1441 aggtccacat cctggccacg ccgaccaagc agccgaagca ggttgtccgg gacgtgcagt
     1501 cggtggcggt ggcgtccttc ggcgtcgagc tggaccgccg gatcatctcc gtcgtccagc
     1561 tcgaggatcg cgcccaggcg ggagatgccg gcggcaactg ctcgacccgg gccgttcaga
     1621 gccagcccat ggctccggag ccggcccgta gggcggcgat cggggacctg gagcctgggg
     1681 ctgcctccag ggcgcgcgtc gtcgtcgacg gggtggctta cgctcggaac ggctaccgct
     1741 gctcggtcga ggtgtccctc cggctcggcg aggagcgggc cgtgggccag gcggaaggcg
     1801 cggcgtcgag cggatcggcc tcccggctgg ccgcggcggc caccctttc
//
LOCUS       DASWGG010000369         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_511892, whole genome shotgun sequence.
ACCESSION   DASWGG010000369 DASWGG010000000
VERSION     DASWGG010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511892"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..>1599
                     /locus_tag="VF954_06700"
     CDS             17..>1599
                     /locus_tag="VF954_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530807.1"
                     /translation="MALAAAAIVLQSTALSDPRSKVSPDQLRAATLPAAAAQPARPVP
                     PPTTAPAATTGPPPTTAPPAPPPPPPPPTAAAYWLASSDGGIFSFGSASYAGGPPGAA
                     LRAPLVTLASTPSGRGYWVVGADGGVFAYGDAPFEGSTGSMDLNRLVVGIAATPSGQG
                     YWLVASDGGVFAFGDAGYYGSSSGLRLRNRIVGMAATPTGKGYWLAGADGGIFAFGDA
                     PYLGSVAAAALNEPVVAVSATPTGRGYWLAGADGGVFGLGDAGYFGSTAALQVRDVAG
                     MAPTPSGRGYWLVGRDGGVFSFGDADFYGSVSDLRLHAPVVAISARRAVLQGAPARHI
                     PAPPSVAGPVGFDVSWPQCAYGLPPGPYGFGIVGVTGGRSLSGNPCLADEWRWAKGNA
                     SRTGLYLNVSYPGSDQWAAGASGPAGTCGPGDLYCQLYNFGANTAVGAVGYARQNGAD
                     APRWWLDVETANPWSGDTAANVQVVAGAVDQLHRMGLQVGLYSTPYQWGAIVGGYQPG
                     LPVWTAGASGLGDVPGYCDG"
BASE COUNT          170 a          607 c          595 g          227 t
ORIGIN      
        1 acgcgtcaga gcagtggtgg ctctggccgc cgcggcgatc gtgctccagt cgaccgccct
       61 gtcggacccc cggtcgaaag tctcgccgga tcagctacgg gccgcgacgc tccctgccgc
      121 cgcggcccag ccggcacgcc cggtgccacc cccgacgacc gcgccagccg cgacgacggg
      181 gccacccccg acgaccgcgc ccccggctcc accgccgccc ccgcctccac cgaccgcggc
      241 cgcctactgg ctggcgtcgt cggacggggg gatcttctcc ttcggctcgg cgtcctacgc
      301 gggtgggcct cccggcgccg ccctgcgggc accgctggtc accctggcgt cgaccccgag
      361 cggcaggggc tactgggtgg tgggagccga cgggggtgtg ttcgcctacg gcgacgcccc
      421 gttcgagggc tcgaccggga gcatggacct caaccggctg gtggtgggca tcgccgccac
      481 gccgagcggg caggggtact ggctggtggc ctccgacggc ggcgtcttcg ccttcggcga
      541 cgccggctac tacgggtcct ccagcggctt gcgcctccgc aaccggatcg tcgggatggc
      601 cgccaccccc acggggaagg gctactggct ggccggggcc gacggcggca tctttgcctt
      661 cggcgatgcc ccctacttgg ggtcggtggc cgcggccgcg ctcaacgagc ccgtggtcgc
      721 cgtctccgcc acccccacgg gccggggcta ctggctggcc ggggcggacg gaggtgtctt
      781 cggcctgggc gatgccggct acttcggctc gaccgccgcg ctccaggtgc gcgacgtggc
      841 cggcatggcg cccaccccgt ccgggagggg ctattggctg gttggccggg acggcggcgt
      901 gttcagcttc ggggacgccg acttctacgg atcggtcagc gacctgcgcc tccacgcgcc
      961 ggtggtcgcc atctccgccc gccgggccgt gctccagggt gctcccgccc ggcacatccc
     1021 ggcgccgccc tcggtggccg ggcccgtcgg cttcgacgtc tcctggcccc agtgcgccta
     1081 cggcctgccg cccggtccct acggcttcgg gatcgtcggg gtgaccggcg ggcggtccct
     1141 gtcgggcaac ccgtgcctgg ccgacgagtg gcgctgggcc aagggcaacg ccagccggac
     1201 cggcctgtac ctcaacgtgt cctacccggg gagcgatcag tgggcggcgg gcgcgtcggg
     1261 cccggccggc acctgcgggc ccggcgacct ttactgccag ctgtacaact tcggggccaa
     1321 caccgccgtg ggtgcggtcg gctatgcccg gcagaacggg gcggatgctc ccaggtggtg
     1381 gctcgacgtc gagaccgcca acccgtggtc gggcgatacc gcagccaacg tccaggtcgt
     1441 ggccggggcc gtcgaccagc tgcaccgcat ggggctccag gtcggcctgt actcgacgcc
     1501 gtaccagtgg ggcgccatcg tcggcgggta ccagccgggc ctgccggtgt ggacggcggg
     1561 cgcgtccggg ctcggcgacg tgcccggata ttgcgacgg
//
LOCUS       DASWGG010000370        18607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_513688, whole genome shotgun sequence.
ACCESSION   DASWGG010000370 DASWGG010000000
VERSION     DASWGG010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 18607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18607
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513688"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VF954_06705"
     CDS             complement(<1..196)
                     /locus_tag="VF954_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530808.1"
                     /translation="MPDLEAFTLDQPAVLFLCVHNAGRSQMALGWFNHLAGNGAVAWS
                     GGSEPAVEVNPAAVAAMAEVG"
     gene            318..1091
                     /locus_tag="VF954_06710"
     CDS             318..1091
                     /locus_tag="VF954_06710"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX9530809.1"
                     /translation="MSHVTDAHRERRIELEGAFNFRDLGGYATAGGGPVRWRRLYRAD
                     GIHKLTPADRGRVRELGLATVVDLRTGAELEERGRFPFIGELEGYHHLPLIDAVWDPA
                     TVEEQVAVMSAAELLVFQYKAMLVEGRAAIAATFELLAEPGSYPLVFHCAAGKDRTGV
                     VAALVLSLLAVSDADVCHDYGLSREAMERMMAWIRTNRPEALDAMVAQPKAILDAPPM
                     AMHLLLDSVRAEFGSVAGYLGDLGLSSRTLGAVRANLVA"
     gene            complement(1088..1501)
                     /locus_tag="VF954_06715"
     CDS             complement(1088..1501)
                     /locus_tag="VF954_06715"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9530810.1"
                     /translation="MDDAAVEVEAAGAVVWRRDPGGGLEVLLVHRPRYDDWSLPKGKL
                     IEGEEALAGALREVEEETGLYGEVGPELAETRYADAKGRDKRVRYWAMRATGGSFQPN
                     DEVDEVRWLALDAAVSLLSHAHDVAVVAGLGSRLG"
     gene            1585..3135
                     /locus_tag="VF954_06720"
     CDS             1585..3135
                     /locus_tag="VF954_06720"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX9530811.1"
                     /translation="MTETTTFTDPTVTPEAVLASLADAGFHLGRPERARRTLLDTFDL
                     RLRRARMRLERRLGAVDELVLTAPGSPPARLGNPCAPERPRRGEDLPAGPFRARLASV
                     LEVRALLPLVTVRGTSRIGVKRDANGQAVVTVVMHQRLTLEGGARARTGAPRWVVEVE
                     PLPGFERAAQRAEALMASLGLRRRPGDVVDLMAAVTGADRPAFDSSPTIALDRHRPAY
                     EAFALVLRHLATSVEANRPGTIDDVDPEFLHDLRVAVRRTRSVLGEARRVLPPDVRSH
                     HRQEFRWLGTATSPVRDLDVYLIEWGSYTAALDDGARAALEPARAHLAARRQTERAAL
                     TRDLRSARYERLVEGWLGWLAAPVPGDGPDASTPIGRVASRRIDAAQEAVLGRGRSIG
                     PGSPAEDLHELRKDAKKLRYLLECFGGLYDPAARQDFVKQLKALQDNLGEHQDAEVHV
                     AQLKELAGDVHGLPITTPGTMLAMGQLTQQLDLVRTGARAEFASRFAAYDTARVRRSL
                     HALLEATP"
     gene            3132..3872
                     /locus_tag="VF954_06725"
     CDS             3132..3872
                     /locus_tag="VF954_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9530812.1"
                     /translation="MKTIATYSIKGGVGKTTSAVNLAHEAARGGARVLLWDLDPQGAA
                     TFFFRVRPKMKGGSERLVGAKGELERHIRSTGIPGVHVVPADFSLRHLDLHLDATKHP
                     TTRLSALLVPLRWRYDLALLDCAPSISLASEGVFAATDALVVPVIPAALSARTLEQLV
                     EFLRERESPPRIMAHFSMVDRRRKLHRELVEGPMAQSSLFLRTVIPSSSLVERMGHER
                     APLAAFAPSSAPARAFAALWAEIADRLW"
     gene            3901..4416
                     /locus_tag="VF954_06730"
     CDS             3901..4416
                     /locus_tag="VF954_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9530813.1"
                     /translation="MTTYDRPFGRYFEDFEPGDIYKHWPGKTVTEYDDHLFCMITMNH
                     HPLHTNAWFAEGETVHKRNVVVGNLVYSLVLGMSVPDVSGSCIANLEVESLTHKLPTF
                     HGDTIYAETRVLDKVESRSKGDRGVVAVETKGFNQRGEEVCYFRRKFMVWKTADAPPR
                     RRPYGDDVWEA"
     gene            complement(4426..5226)
                     /locus_tag="VF954_06735"
     CDS             complement(4426..5226)
                     /locus_tag="VF954_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9530814.1"
                     /translation="MTAALDLSTIPTTQVTPASAARDIAVVTRRNLRRLARTPQLLAF
                     STIQPVMFTLLFRYVFGGAIRTPGMPYVDYLMPGIFVQVVLFGGTTAVALATDLTSGM
                     VDRFRSLPMARSAVLAGRTFADLARNVITLVILLVVGSLVGFRFHNGPVAGLAGVLLV
                     LAFGYAFSWLSAVVGLTVKDPETAQIAGLLPVFPFIFASSAFVPVRSMPGWMQAFAKV
                     QPVSVLVNSVRALTQGGPIYHSLWQAVAWIVVFLVFFVPLAVRRYRKL"
     gene            complement(5223..6218)
                     /locus_tag="VF954_06740"
     CDS             complement(5223..6218)
                     /locus_tag="VF954_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9530815.1"
                     /translation="MDQAAMIEASGIRKAFGATQALDGVDLVAEPGRVLALLGPNGAG
                     KTTLVRILTTLLQPDSGWARIGGLDTVKDAARLRSVVGLSGQFAAIDDLLTGRENLEM
                     IGQLYHLSNADARGRARRLLDEFTLGDAADRLAKTYSGGMRRRLDLAASLIAEPPVLI
                     LDEPTTGLDPRTRIDVWNIIDKLVGAGTTVLLTTQYLEDAERLAHRIVVIDQGVVIAE
                     GTANELKDKVGGDLVEVRPGQAEELDRVRSAITPLAAGALIEDRERLRITIPAPDGVE
                     TLRHTLRLLDEAGVTVHDIGIRRPSLDDVFLALTGHAASADGGEVAGAAPPGPPR"
     gene            complement(6376..6894)
                     /locus_tag="VF954_06745"
     CDS             complement(6376..6894)
                     /locus_tag="VF954_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6616..6618),aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HEX9530816.1"
                     /translation="MHRFVLDPTSEREPAARARVTRPASLEGLRVGLLDISKPRGDVF
                     LDTLDALLTSRGAQVARYKKPTFTKPAPVDLRHEIAIECDVVIEALADUGSCTSCSVH
                     DITDLEGRGVAGVFIASTEFELAASAQAAALGFSPAAVFVAHPIQDRTDEEMRALATG
                     CVDDVVAAVTGA"
     gene            complement(6903..8312)
                     /locus_tag="VF954_06750"
     CDS             complement(6903..8312)
                     /locus_tag="VF954_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9530817.1"
                     /translation="MEIATATVANLDGLVAVVKADCPTCRLVAPVLTALGRRVAVYSE
                     DDDAGLELSWHLDLDTVPTLLRFESGTEKGRIVGWDREAWRTFTCDPALGDGLPEYRP
                     GCGSKSVEPGVASLLAERFSGAVLRSRRVELGAAEDESEALFDRGWTDGLPVVAPTPR
                     RVLAMLAGTSLPPDQVVAVVPPDLVPVTVEKVAVNAVLAGCRPEYLPFVLAAVSAACT
                     DEFNVHGLLATTYFSGPMVVANGPLTRAIGMNSGGNALGQGNRANATIGRALQLVVRN
                     VGGGRPGGVDRATLGNPGKYTFCFAEDEEGSPWESLAVERGFPATASTVTLFAGAGVT
                     PVVDQLSRSPESLARSFAACLRMVNHPKLVMGFDAVLVVSPEHAGIFRMAGWDKARLR
                     AFLMELLQLDGASLVRGADGIDEGVPARMAGAVLPKFRPDGLLFVHAGGTAGRFSAII
                     GGWVSGPTGSSPVTMEVKP"
     gene            8411..9508
                     /locus_tag="VF954_06755"
     CDS             8411..9508
                     /locus_tag="VF954_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX9530818.1"
                     /translation="MAHELLDRCLHQLAEADGSDLHIKVGSPPRVRVNGDLRKLVEYS
                     PLSAQDTADMAAAIMRPEVAEQFTERHEADFAYAVQGLGRFRANVFRQRGSVAMVFRR
                     VRTSAATFSELGLPDVVMRLANENRGLVLVTGPTGSGKTTTLAAMIDHINRSRECHIV
                     TIEDPVEVLHRDELAEINQREIGFDTLDFASAMRAAMRQDPDVILVGEMRDPETVSAA
                     LSAAETGHLVLSTLHTIDSTETVNRIVDFFPPFQQNQIRVALAGALKGTICQRLVPKS
                     DGRGRVAALEVMVVNGRIQQCIVDPGRTGDIKEIVADGEYYGMQTFDQSLVRLYELGQ
                     IDLRGAMVAATSPHDLKVMLQGRGLVGTGAR"
     gene            9612..10442
                     /locus_tag="VF954_06760"
     CDS             9612..10442
                     /locus_tag="VF954_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9530819.1"
                     /translation="MTFYGVRGSCPCPSESNARYGGNTACVALEAEEGDEPPIILDLG
                     TGLRRLGEGQPCDGTFRATALVTHLHWDHVQGLPFFAPVDRVGATFDIYGPEQEEGPL
                     GEVFGGFMRPPYFPVTFTELRGDIRFHGVSTDELVIGGAKVMVRPVPHVGATVGYRIE
                     WGGSSVAYISDHQAPLDLRRVADSVLELCDGVDLVIHDAQYTPAEFAERAHWGHCTID
                     YAVLVAKEAGARRLALFHHDPAHDDDRMDGLLEGARCLGAMAGLDEVLAAAEGLHLRV
                     "
     gene            10439..10957
                     /locus_tag="VF954_06765"
     CDS             10439..10957
                     /locus_tag="VF954_06765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9530820.1"
                     /translation="MRAIDPDDIARTGAPTFEEAHFRQVLGHFATGITIITTVDEDGP
                     VGFTCQSFMSLSMDPPLVGFSPSRTSATWPRIEKTEHFCVNILSDRQEDVCRVFATKS
                     QDKFSGVGWRRGRTGAPVLHEVLGWIEASIQTVHDAGDHLLVVGRVLDLGMGQEGRPL
                     LFYRGGYGRFEA"
     gene            11109..12938
                     /locus_tag="VF954_06770"
     CDS             11109..12938
                     /locus_tag="VF954_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9530821.1"
                     /translation="MELKALGQSGVIRVATGDVARHRERNRRNRVGRVAGVLGVVAVF
                     VWGRLLSGQPVHPGWPAVHLPAGAEAYLPAVVLVLLLAVVIVVPMLGAGRSPHVLYRA
                     SEIDTSLDDVKGLDVVKEEVVKSLNLFLAHKTFHDRMGGTPRRAVLFEGPPGTGKTYM
                     AKAMAREAGVPFLFVSSSAFQSMYYGQTNRKIRSYFKALRRYARQEGGAIGFIEEIDA
                     IGAARSGMGSSTQREGISGVVNELLIQLQSFDTPTAGGRMWGALVDRVNRWLPPERQV
                     RKPKAVPANILVIGATNRAADLDAALLRPGRFDRSIYFDLPSRSGRREIIDYYLARKA
                     HSRDLDDPARRDTLAAMTFGYSPVMIEHLLDEALVWALRRGAARLSWEDIQHAKMTEE
                     IGLAQPVEYTEAERRTIATHESGHATVAWLVGKGRKLEVLSIIKRKDALGLLAHSDLE
                     ERYTKTESEIHALIEIAFGGMVAEELFFGETSSGVAGDLQAATSAAAQMFGTFGMSGS
                     LISYEAVGGPGGANLTAKVLSSDEGKEAVNGILERSREDVRRMLNENRHVVEALRDAL
                     LERDELIGEEIGQVIAHAIAPVVDLREAQGAQSAERPAASAGD"
     gene            complement(13008..15083)
                     /locus_tag="VF954_06775"
     CDS             complement(13008..15083)
                     /locus_tag="VF954_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9530822.1"
                     /translation="MAVTDRPPRAAKRPHALVAHGDERIDEWYWLRDRDDPEVTAHLE
                     AENDFTAAQMAGTKDLQRQLFEEIVGHVQETDLTVPARKGPWLYYARTIEGHQYAIHC
                     RRPPGPEGEGAEAVLLDENAEAGDGDFFDLGNFEVSPGHGLVAWAADTNGGERFTLRF
                     RDLGTGADLDDVVEDTYYSVAWANDSKTVFYTRPDEAMRPWQVWRHRLGTPATADVLV
                     FQEDDEHFFLEVGRTRDDAFVVLQAGSKITTEVRVLDAGDPEGDFRVVARRQHGVEYA
                     IDHHGDRFLVVTNAGGRENFAVMEAPEATPGPEHWRELLPYDPEVRVEDVDAFAHHLV
                     VWERAAGLRTIRVVDLDDMSSYVIDQPEAVYGTWAGANPEFETATLRYQYASMVTPMS
                     VIDFDLRTRRATLLKRQPVPGYDPAPYVTERVWATAGDGARVPISLVYREGLARDGSA
                     PCLLYGYGSYEHSLEPGFSSVRLCLLDRGFVYAVAHVRGGGEMGRPWYEQGKMLHKRN
                     TFTDFVAAADHLVAGGWTSPPRLAARGGSAGGLLMGAVANLRPDLFGAIVAEVPFVDC
                     LTTILDDTLPLTVMEWEEWGNPVADPEVYAYMRSYAPYDNVSAQDYPALLVTAGLNDP
                     RVSYWEPAKWVARLRDRATGAMPILLKTEMGAGHQGPSGRYDAWRDEAFVLAFVLRAL
                     GVEDAGGGA"
     gene            15167..16951
                     /locus_tag="VF954_06780"
     CDS             15167..16951
                     /locus_tag="VF954_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9530823.1"
                     /translation="MSTSPMAATRSGGALMRTTITRYWRGVVIGAGAGLVWTAAKVSI
                     PDLIGHAIDKGVVHPQGHTLLTYALVIAAVGIVQGGCTGLRRYYAFWVAYRVETDLRH
                     RLFAHIQRLHLAFHDHAQTGQLMSRSATDLQQAQGFVSMIPITISSAVTVVAVAAIML
                     ATNVKLTFLALCALPLINLVAKTFSSRVYPVSLGLQQELAGVATVVEETVTGIRAVKG
                     FGAEAIQARVLRQRTDRVWHRAMAAARIRATYAPLLDVLPAVGMVLVLWYGGHQVLSR
                     HLSVGQLTEFVLYVTLLVNPLRMLGQLVAQAQRAIASADRVGEILSTEPEITSSPKAP
                     KLPPGGGALRFDDVRFAYQPGAEPVLDGFSLAVEPGESVALVGPTGSGKTTVARLIPR
                     FYDIQHGTITIDGADIRKVRLPDLRRNVGIVFEDTFLFTDSIRANIAFAEPDAPFDQV
                     VRAARSAGADEFIRAMPEGYDTIIGERGFSLSGGQRQRIALARAIVADPRVLILDDAT
                     SSVDPTKEHEIRGALLEVMRGRSTIVIAHRPATIALADRVILLDGGRVVAQGTHEELL
                     ETSERYREVLARAEGSHNVAAGLSGDAG"
     gene            16951..>18607
                     /locus_tag="VF954_06785"
     CDS             16951..>18607
                     /locus_tag="VF954_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9530824.1"
                     /translation="MGRWSNVAVDEEDKLDRKGATRVMRRSFRMLRRYRPLVVASALV
                     MIGYTAGLVAGPVAIQFGIDQGLGKHSGHDLDLAVVGYVAASVIVLVCGRGQIRLVTR
                     LGENFLRDLRVRVFDHILALSMGFFDKEQTGKLVARMTSDIDAMEDLIQLGLVQFVTN
                     GLLFAATIGVMVVESWRLTLVCMVSVPLVVVESRKFRRRSNEAYLNVRDRIGQTMSTI
                     QEGISGVRVIQAFGREDLQVERFSNRNQAQLDANVRAVGISARYFPVIEFSGVATMAA
                     LVGIGGYLVHTKATTLGTVALFFVLLQLLLDPIIQLSQLFNLVQSSGAALNKLFGLLD
                     TDSEVKERPGAVDLPDGGAFEVRGVGFAYGPDLPPVLDGVDLTVASGERLALVGPTGA
                     GKSTLAKLISRFYDPTEGAVTYGGVDLRDATLGSLRQRICVVPQEGFLFHGTIMDNVR
                     LGRQGATDEEVRAALGTIGIGDRFDALPEGLETEVRERGSRLSAGERQLVSLARAALA
                     DPQVLVLDEATSNLDPGTEVIVEGAVNALMTGRTVIIIAHRLST"
BASE COUNT         2669 a         6834 c         6474 g         2630 t
ORIGIN      
        1 tgcccacctc ggccatggcg gccaccgctg cggggttgac ctcgacggcg ggctccgagc
       61 cgcccgacca ggccacggcc ccgttcccgg ccaggtggtt gaaccagccg agcgccatct
      121 gcgatcgccc ggcgttgtgc acgcacagga agaggacggc gggctgatcc agtgtgaagg
      181 cttcgagatc gggcacggca gctcccttcc caccggcgaa cctaccggct ggttcatagc
      241 ccggccgacg cccacggttc acctccgcgt cacccgacgg tagacccgtg gccagcaacg
      301 ccgggtacgg tcgagacgtg agccacgtga ccgacgccca ccgcgagcgc cggatcgagc
      361 tggagggcgc gttcaacttc cgggatctcg gcggctacgc caccgccggc ggcgggccgg
      421 tgcgctggcg ccgcctgtac cgcgccgacg gcatccacaa gctgacgccc gccgaccgcg
      481 gccgggtacg cgagctcggc ctggcgacgg tcgtggacct gcggacgggc gcggagctcg
      541 aggagcgcgg ccggttcccg ttcatcgggg agctggaggg ctaccaccac ctcccgctga
      601 tagacgccgt gtgggacccc gccaccgtgg aggagcaggt ggcggtcatg tcggcagccg
      661 agctcctcgt cttccagtac aaggcgatgc tcgtcgaagg gcgggccgcc atcgccgcca
      721 cgttcgagct gctcgccgag cccggctcct accccctcgt ctttcactgc gcggcaggca
      781 aggatcgcac gggcgtggtc gccgccctgg ttctcagcct gctggccgtg agcgacgccg
      841 acgtctgcca cgactacggt ctcagccggg aggcgatgga gcggatgatg gcctggatca
      901 ggaccaaccg gcccgaggcc ctcgacgcca tggtcgccca gcccaaggcg atcctcgatg
      961 ccccgcccat ggccatgcat ctgctcctcg actcggtgcg cgccgagttc ggctccgtcg
     1021 ccggctacct cggcgacctc ggcctctcga gccgaacgct cggcgcggtc cgcgccaacc
     1081 tggtcgccta accgagccgg ctgccgagcc cggccaccac ggccacgtcg tgggcgtggg
     1141 acaggagcga gacggccgcg tcgagtgcca gccagcggac ctcgtccacc tcgtcgttgg
     1201 gctggaaaga tcccccggtg gcgcgcatgg cccagtaccg gacccgcttg tcgcggccct
     1261 tggcgtccgc gtagcgcgtc tcggccagct cgggaccgac ctcgccgtac aggcccgtct
     1321 cctcctcgac ctctcggagc gccccggcca gggcctcctc cccctcaatg agcttgccct
     1381 tgggcaggga ccagtcgtcg tatcgcggcc ggtggaccag caggacctcg aggcccccgc
     1441 ccgggtcccg gcgccacacg acggccccag ccgcttcgac ctccaccgcg gcgtcgtcca
     1501 tggacgggag gctaggggcg cgccgggtag cctggctcgg aggtgcgagc tgctggagcc
     1561 cgcagtcgtc ccgcagtcgt tccgttgaca gagaccacca ccttcacgga tcccaccgtc
     1621 acgccggagg cggtcctcgc ctccctggcc gacgccggct tccacctcgg ccgcccggaa
     1681 cgggcccggc gcaccctcct cgacaccttc gacctccggc tgcgccgtgc ccgcatgcgc
     1741 ctcgaacgcc ggctgggcgc ggtcgacgag ctggtcctga cggcgccggg gtcgcccccg
     1801 gcccggctgg gcaacccgtg cgccccggag cgcccgcgcc ggggtgagga cctccccgcc
     1861 ggcccgttcc gggcccgcct ggcctcggtc ctggaggttc gggcccttct ccccctcgtc
     1921 acggtgagag gaacctcgcg catcggggtg aagcgcgacg ccaacggcca ggccgtcgtc
     1981 accgtcgtga tgcaccagcg gctgaccctc gaaggcggcg cccgcgctcg caccggagcg
     2041 ccgagatggg tcgtcgaggt cgaacccctc ccgggtttcg agcgcgccgc ccagcgggcc
     2101 gaggcgctca tggcgtcgct cggcctcagg cggcgcccgg gcgacgtcgt cgatctgatg
     2161 gcggcggtga caggcgcgga ccgccccgca ttcgacagct caccgacgat cgcgctcgat
     2221 cgccaccggc cggcctacga ggccttcgcc ctggtgctgc ggcacctggc cacctccgtc
     2281 gaggccaatc ggccgggcac catcgacgac gtcgaccccg agttcctcca cgatctccgc
     2341 gtcgcggtcc ggcgtacccg ctcggtgctg ggcgaggcca gacgggtgct gccgcccgac
     2401 gtgcgatcgc accatcgaca ggagttccgg tggctgggca cggcgacgag cccggtgcgc
     2461 gacctggacg tgtacctcat cgagtggggc tcctacacgg cggctctcga cgacggggcc
     2521 cgggccgcgt tggagcccgc ccgcgcccac ctggcggccc ggcggcagac ggagcgtgcc
     2581 gccttgacgc gggacctgcg ctccgcccgg tacgagaggc tggtggaagg ctggctcggg
     2641 tggctggcgg cgcccgtgcc cggggacggc cccgatgcgt ccacgccgat cggacgggtc
     2701 gcgtcccgtc gcatcgacgc ggcccaggaa gcggtcctcg ggcggggccg gtcgatcggc
     2761 ccgggctctc ccgcagagga tctccacgag ctgcgcaagg acgccaagaa gctgcgctac
     2821 ctgctggagt gcttcggcgg gctgtacgac ccggccgcgc gccaggactt cgtcaagcag
     2881 ctgaaggccc tccaggacaa cctcggcgag caccaggatg ccgaggtcca cgtggcccag
     2941 ctcaaggagc tggccggtga cgtccacggc ctgccgatca ccaccccggg caccatgctg
     3001 gccatgggcc agctcaccca gcagctcgac ttggtccgca ccggcgcccg ggcggagttc
     3061 gcctcccggt tcgcggccta cgacaccgcc agggtgaggc gctccctgca cgccctgctc
     3121 gaggcgacac cgtgaaaacc atcgcgacct acagcatcaa gggcggggtg ggcaagacca
     3181 cgtccgcggt caacctggcg cacgaggccg cccgcggcgg ggcccgggtg ctgctctggg
     3241 acctcgaccc ccagggggcg gcgaccttct tcttccgcgt ccggcccaag atgaagggcg
     3301 ggagcgagcg cctggtcggc gccaagggcg agctggagcg ccacatccgg tccacgggca
     3361 tcccgggcgt gcacgtggtg ccggccgact tctcgctgcg ccacctcgat ctccatctcg
     3421 acgcgaccaa gcaccccacc acgcggctgt ccgccctgct cgtcccgctc cggtggcgct
     3481 acgacctggc gctgctcgac tgcgccccga gcatctcgct cgcgagcgaa ggcgtctttg
     3541 ccgcgaccga cgccctcgtc gtcccggtca tcccggccgc gctgtcggcc cgcaccctcg
     3601 agcagctggt cgagttcctc cgggagcgcg agagcccccc gcgcatcatg gcccatttct
     3661 ccatggtcga ccggcgccgc aagctccacc gggagctcgt cgagggcccg atggcacaat
     3721 cgtcgctctt tctccgaacc gtgatcccga gctcgagcct ggtcgagcgc atgggccacg
     3781 agcgggcccc gctcgccgcc ttcgccccct cgagcgcacc ggcccgggcc ttcgccgcgc
     3841 tgtgggccga gatcgccgac cggctgtggt gacggcggcg gcgctaacct gcgcggccgt
     3901 atgacgacct acgaccggcc cttcggccgc tacttcgagg acttcgagcc cggcgacatc
     3961 tacaagcact ggccgggcaa gaccgtcacc gagtacgacg accacctctt ctgcatgatc
     4021 accatgaacc accacccgct gcacaccaac gcctggttcg cggagggcga gaccgttcac
     4081 aagcgcaacg tcgtggtcgg caacctggtg tactcgctgg tgctggggat gagcgtcccc
     4141 gacgtgagcg ggtcgtgcat cgccaacctc gaggtggagt cgctgaccca caagctgccg
     4201 acgttccacg gcgacaccat ctacgccgag accagggtgc tcgacaaggt cgagtccagg
     4261 tccaagggcg accggggcgt ggtcgccgtc gagaccaagg gcttcaacca gcggggcgag
     4321 gaggtgtgct acttccggcg caagttcatg gtgtggaaga cggccgacgc cccgccccgg
     4381 cggcgcccct acggtgacga cgtctgggag gcctgagcgc agcgctcaca gcttccggta
     4441 gcggcgcacg gccaggggaa cgaagaacac caggaacacc acgatccagg ccaccgcctg
     4501 ccagagcgag tggtagatgg gcccgccctg ggtcagggcc cggacggagt tgacgagcac
     4561 gctcaccggc tggaccttgg cgaacgcctg catccagccc ggcatgctcc ggaccggcac
     4621 gaacgcggag ctggcgaaga tgaaggggaa gaccggcagg aggcccgcga tctgcgccgt
     4681 ctccgggtcc ttgacggtga ggccgaccac cgccgagagc cacgagaacg cgtacccgaa
     4741 ggccaggacc aggagcacgc cggccaggcc ggccaccggg ccgttgtgga agcggaagcc
     4801 gaccagcgac cccaccacga gcaggatcac cagggtgatg acgttgcggg ccaggtccgc
     4861 gaaggtgcgg ccggccagga ccgccgagcg cgccatgggc agcgagcgga agcggtcgac
     4921 catccccgac gtgaggtcgg tggcgagcgc gacggcggtg gtccccccga agagcacgac
     4981 ctgcacgaag atccccggca tcaggtagtc gacgtagggc atgccgggcg tccgtatggc
     5041 cccgccgaac acgtacctga acaggagcgt gaacatcacc ggctggatgg tggagaaagc
     5101 cagcagctgt ggcgtgcggg ccaggcgccg gaggttgcgc ctggtgacca cggcgatgtc
     5161 cctcgccgcc gaggccgggg tgacctgggt ggtcgggatg gtcgacaggt cgagggcggc
     5221 ggtcatcgtg ggggtcccgg tggggccgcg ccggcgacct cgccgccgtc ggctgaggcg
     5281 gcgtggccgg tcagcgccag gaacacgtcg tcgagcgacg ggcgccggat gccgatgtcg
     5341 tggacggtga cgccggcctc gtccagcagc cgcagggtgt ggcgcagcgt ctcgacgccg
     5401 tcgggggccg ggatggtgat gcgcaggcgt tcgcggtcct cgatcagcgc ccccgcggcg
     5461 aggggggtga tggcggagcg cacccggtcg agctcctcgg cctgcccggg ccggacctcg
     5521 accaggtcgc caccgacctt gtccttgagc tcgttggcgg tgccctctgc gatcaccacg
     5581 ccctggtcga tcaccacgat gcgatgggcc aggcgctcgg cgtcctcgag gtactgggtc
     5641 gtcagcagca cggtcgtgcc cgcgcctacc agcttgtcga tgatgttcca gacgtcgatc
     5701 cgggtgcggg ggtcgaggcc ggtcgtcggc tcgtcgagga tcagcacggg cggctccgcg
     5761 atgaggctcg ccgccaggtc cagacgccgg cgcatcccgc ccgagtacgt cttggccagc
     5821 cggtcggcgg cgtcgccgag cgtgaactcg tcgaggagcc tgcgagcgcg acctcgggcg
     5881 tcggcgttgc tcaggtggta gagctggccg atcatctcca ggttctcccg gccggtgagc
     5941 aggtcgtcga tggccgcgaa ctggccggag agcccgacca ccgaccgcag gcgggccgcg
     6001 tccttgaccg tgtcgagccc gccgatgcgg gcccacccgc tgtccggctg gaggagggtg
     6061 gtcaggatgc gcacgagcgt ggtcttgccg gcgccgttcg ggcccagcag ggccaggacc
     6121 cggccaggct cggcgacgag gtcgacgccg tcgagcgcct gcgtcgctcc gaaggccttg
     6181 cggatgccgg aagcctcgat cattgcggcc tgatccaccc tgggcaggct acgcggcccc
     6241 gggcgggcgc gcgagcgtgt cgttcgtcac accaggggcg gctgcccgca actgggtgcc
     6301 caaatccacc gtcgcggtgg gttaccgtcc ccagctcgac gcgggcccag atcggcgggc
     6361 ccctggcggc aggccctacg cgcccgtgac cgccgcgacc acgtcgtcca cgcagcccgt
     6421 ggccagggcg cgcatctcct cgtcggtacg gtcctggatg ggatgggcca cgaacaccgc
     6481 ggccggcgag aaccccaacg ccgcggcctg ggcggacgcc gccagctcga actcggtcga
     6541 cgcgatgaac accccggcca cgccccggcc ctcgagatcg gtgatgtcgt gcacactgca
     6601 cgacgtgcag ctgcctcagt cggccagggc ctcgatcacc acgtcgcact cgatggcgat
     6661 ctcgtggcgc aggtccacgg gcgccggctt cgtgaaggtc ggcttcttgt agcgggcgac
     6721 ctgggcgccc cgagacgtca gcagcgcgtc gagcgtgtcc aggaacacgt cccctcgcgg
     6781 cttggagatg tccagcagcc cgacccgcag gccctccagc gacgccgggc gggtcacccg
     6841 ggcccgggcg gccggctccc gctcgctcgt cgggtccagc acgaaacggt gcagcgcctc
     6901 gttcacggct tcacctccat ggtcaccggg ctactcccgg tcggaccgct cacccagccc
     6961 ccgatgatgg ccgagaagcg cccggccgtc ccacccgcgt ggacgaacag cagcccgtcc
     7021 ggccggaact tgggcagaac ggccccggcc atccgcgcgg gcactccctc gtcgatcccg
     7081 tccgcacccc gcaccagcga cgccccgtcg agctggagca gctccatcag gaaggcgcgc
     7141 agccgggcct tgtcccaccc ggccattcta aagatgccgg cgtgctcggg tgagacgacg
     7201 agcacggcat cgaagcccat gacgagcttg gggtggttga ccatccgcag gcaggccgcg
     7261 aacgagcggg ccagcgactc cggcgagcgg gagagctggt ccacgaccgg cgtgacgccc
     7321 gcgcccgcga agagggtgac cgttgatgcc gtggccggga agccccgctc gacggccagg
     7381 gactcccacg gggagccctc ctcgtcctcg gcgaagcaga atgtgtactt gcccgggttg
     7441 cccagggtgg cgcggtcgac cccgccgggc ctgccgccgc ccacgttgcg cacgaccagc
     7501 tggagggccc ggccgatggt ggcgttggcc cggttgccct ggccgagggc gttgcccccg
     7561 gagttcatgc cgatggcccg ggtgagcggc ccgttggcca cgaccatggg cccggagaag
     7621 taggtggtgg cgagcaggcc gtgaacgttg aactcgtcgg tgcacgcggc cgacacggct
     7681 gccagcacga acggcaggta ctcgggccgg cagcccgcca gcacggcgtt gaccgcgacc
     7741 ttctccaccg tgacgggcac gagatcgggc ggcaccaccg cgacgacctg gtcgggtggg
     7801 agcgacgtcc cggccagcat ggccagcacg cgccggggcg tcggcgcgac cacgggcagg
     7861 ccgtcggtcc agccccggtc gaagagggcc tctgactcgt cctccgccgc gcccagctcg
     7921 acccggcggc ttcgcaggac cgcgccgctg aaccgttcgg ccagcagcga agccaccccg
     7981 ggctccacgg acttggaccc gcaccccggc cggtactcgg gcaggccgtc gccgagcgcc
     8041 gggtcgcagg tgaaggtccg ccacgcctcc cggtcccatc cgacgatgcg acctttctcc
     8101 gtccccgact cgaaccgcag gagcgtgggc acggtgtcga gatcgagatg ccacgacagc
     8161 tccaggccgg cgtcgtcgtc ctcgctgtag accgcgactc gccggccgag ggcggtgagc
     8221 acgggcgcga ccagccggca cgtcgggcag tcggccttca ccaccgccac cagcccgtcc
     8281 aggttggcca cggtcgccgt cgcgatctcc acggccggac cctaaccccc cgaggctgcg
     8341 ctcgcaccag gtggccggtc ccgcaaggcg gcgctcaacg ccccccgtcc ccgtgccgat
     8401 acaggtaccc atggcgcatg agctgctgga tcgatgtctg caccagctgg cggaagcaga
     8461 cgggtccgat ctgcacataa aggtcgggtc ccctccgcgg gtgcgggtca acggcgacct
     8521 gcgcaagctg gtggagtatt cgccgctcag cgcccaggac accgccgaca tggccgccgc
     8581 catcatgcgg cccgaggtgg cggagcagtt caccgagcgc cacgaggccg acttcgccta
     8641 cgccgtgcag ggcttgggcc ggttccgcgc caacgtgttc cgccaacggg gatcggtcgc
     8701 catggtcttc cgccgggtcc ggacctcggc ggccacgttc tccgagctgg gcctgcccga
     8761 cgtggtcatg cgcctggcca acgagaaccg gggcctcgtg ctcgtgaccg gcccgaccgg
     8821 gtcggggaag acgaccacgc tcgcggccat gatcgaccac atcaaccgca gccgcgagtg
     8881 ccacatcgtc accatcgagg accctgtgga ggtgctccac cgcgacgagc tggccgagat
     8941 caaccagcgg gagatcggct tcgacaccct cgatttcgcc tcggccatgc gggcggcgat
     9001 gcggcaggac ccggacgtga tcctcgtcgg tgagatgcgc gacccggaga cggtgtcggc
     9061 ggccctgagc gcggccgaga ccggccacct ggtgctgtcc accctccaca ccatcgactc
     9121 gaccgagacg gtgaaccgca tcgtcgactt cttccccccg ttccagcaga accagatccg
     9181 ggtggcgctc gccggggcgc tgaagggcac catctgccag cgcctcgtgc ccaagtccga
     9241 cggccgggga cgggtcgccg ccctcgaggt catggtggtc aacggccgga tccagcagtg
     9301 catcgtcgac cccggccgca ccggcgacat caaggagatc gtggccgacg gcgagtacta
     9361 cgggatgcag accttcgacc agtccctcgt gcgcctctac gagctgggcc agatcgacct
     9421 gcgcggggcc atggtcgccg ccaccagccc ccatgacctg aaggtcatgc tccagggccg
     9481 gggtctggtc ggcaccggcg cccggtagca cctccggcgc cgctcacacc cggtgaactt
     9541 cgtcacacga gggcccccaa cgttgacgtc tgggtaacct cgtggaaacg caggggggaa
     9601 ccactctgaa cgtgaccttt tacggcgtca ggggctcctg cccctgtcct agcgagtcca
     9661 atgcccggta cggcggcaac acggcatgcg tcgcgctcga ggcggaggaa ggcgacgagc
     9721 ctccgatcat cctcgacctc gggacggggt tgcgccggct gggcgaaggt cagccctgcg
     9781 acggcacctt cagggccacc gccctcgtca cccacctgca ctgggaccac gtgcagggtc
     9841 tgcccttctt cgcgcccgtc gaccgggtgg gggcgacgtt cgacatctac ggcccggagc
     9901 aggaggaggg cccgctcggc gaggtgttcg gcggcttcat gcggcccccg tacttcccgg
     9961 tgaccttcac cgagctgcgg ggggacatcc gcttccacgg cgtctccacc gacgagctgg
    10021 tcatcggggg cgccaaggtc atggtccgcc cggtgcccca cgtcggggcg acggtcgggt
    10081 accgcatcga gtggggaggc tccagcgtgg cctacatcag cgaccaccag gcgcccctcg
    10141 acctgcgccg ggtggccgac agcgtcctcg agctgtgcga cggggtggac ctggtgatcc
    10201 acgacgccca gtacacgccg gccgagttcg ccgagcgggc ccactggggc cactgcacga
    10261 tcgactacgc cgtgctggtc gccaaggagg ccggggcgcg ccgcctggcc ctcttccacc
    10321 acgacccggc ccacgacgac gaccgcatgg acggcctgct cgagggcgcc cgctgcctcg
    10381 gtgccatggc cggcttggac gaggtgctcg ccgctgcgga aggcctgcac ctgagggtgt
    10441 gagagccatc gaccccgacg acatcgcccg gaccggcgcg cccaccttcg aggaggccca
    10501 cttccgccag gtcctcgggc atttcgccac cggcatcacg atcatcacca cggtcgacga
    10561 ggacggcccg gtcgggttca cctgccagtc gttcatgtcg ctgtcgatgg acccgcccct
    10621 ggtggggttc tcgccctccc gcacgtccgc gacgtggccc cggatcgaga agacggagca
    10681 cttctgcgtc aacatcctga gcgacagaca ggaggacgtc tgccgcgtgt tcgccaccaa
    10741 gagccaggac aagttctcgg gggtgggttg gcggcggggc cggaccgggg cccccgtcct
    10801 gcacgaggtg ctgggctgga tcgaggccag catccagacc gtgcacgacg ccggcgacca
    10861 cctcctcgtc gtgggccggg tcctcgacct gggcatgggc caggagggcc gtcccctgct
    10921 cttctaccgg ggcggctacg gacgcttcga ggcctgacgg cggccgcgga cggacaattg
    10981 ccgcgagacg ggaccgcggc gcagagcggc gcatatcggc gagacggggg gcttcccttg
    11041 agcgaatttc tcacgtaacg tggtcgcaat cccggtttgg gcggcacgaa cgaggacgga
    11101 gcgtggtcat ggagctgaag gcgctggggc agagcggggt gatccgggtc gctacgggcg
    11161 acgtggcccg gcaccgggag cgcaaccgcc gtaaccgggt gggacgggtg gcgggcgtgc
    11221 tgggcgtggt ggcggtgttc gtgtggggcc ggctgctgtc cggccagccc gtgcacccag
    11281 gctggcccgc cgtccatctg cccgccgggg ccgaggccta cctgccggcg gtggtcctgg
    11341 tcctgctcct cgccgtggtg atcgtggtgc cgatgctggg agccgggcgg tcgccgcacg
    11401 tgctgtaccg cgccagcgag atcgacacct cgctggacga cgtcaagggc ctcgacgtgg
    11461 tgaaggaaga ggtggtcaag agcctcaacc tcttcctggc ccacaagacc ttccacgacc
    11521 gcatgggcgg cacgccccgg cgggcggtgc tcttcgaggg ccctcccggc accggcaaga
    11581 cgtacatggc gaaggccatg gcgcgggagg cgggtgtgcc cttcctcttc gtgtcctcct
    11641 cggccttcca gtcgatgtac tacggccaga ccaaccggaa gatccgctcc tacttcaagg
    11701 ccctgcgccg ctacgcccgc caggaggggg gcgccatcgg cttcatcgag gagatcgacg
    11761 ccatcggcgc ggcccgctcg ggcatgggct cctcgaccca gcgcgagggc atctcgggcg
    11821 tggtcaacga gctgctcatc cagctccagt ccttcgacac cccgaccgcg ggcggccgca
    11881 tgtggggcgc gctcgtcgac cgggtcaacc ggtggctgcc ccccgagcgc caggtccgca
    11941 agcccaaggc cgtcccggcc aacatcctgg tcatcggggc gaccaaccgg gccgccgacc
    12001 tcgacgccgc cctgctgcgc cccggccggt tcgaccgctc catctatttc gacctgccca
    12061 gccggtcggg ccggcgcgag atcatcgact actacctggc caggaaggcg cattcccgcg
    12121 acctcgacga tccggcccgc cgcgacaccc tggccgccat gaccttcggc tactcgcccg
    12181 tcatgatcga gcacctgctg gacgaggccc tggtatgggc gctgcggcgg ggcgccgccc
    12241 ggctgtcgtg ggaggacatc cagcacgcca agatgaccga ggagatcggc ctggcccagc
    12301 cggtcgagta caccgaggcc gagcgccgca ccatcgccac ccacgagagc gggcatgcga
    12361 cggtggcctg gctggtgggc aagggccgca agctcgaggt cctgtcgatc atcaagcgca
    12421 aggacgccct cggcctgctc gcccactccg acctggagga gcgctacacc aagacggagt
    12481 ccgagatcca cgccctcatc gagatcgcgt tcggcggcat ggtcgccgag gagctgttct
    12541 tcggcgagac cagctcggga gtggcggggg acctgcaggc cgccacgtcg gcggcggccc
    12601 agatgttcgg gaccttcggg atgtccggct cgctcatctc ctacgaggcg gtcggcgggc
    12661 ccggcggggc caacctcacg gccaaggtgc tcagctcgga cgagggcaag gaggcggtca
    12721 acggcatcct cgagcgcagc cgggaggacg tgcggcggat gctcaacgag aaccgccacg
    12781 tcgtcgaggc cctccgcgac gccctgctcg agcgcgacga gctcatcggc gaggagatcg
    12841 gccaggtcat cgcccacgcc atcgccccgg tcgtcgacct ccgggaggcc cagggcgcgc
    12901 agagcgccga gcgcccggcg gccagcgcgg gcgactgatc ccgacgttcc tggcaccgaa
    12961 aggtggggtt ttccacgcct ttcggtgcca ggaacgacac gcgcgcccta cgcgccgccg
    13021 ccggcgtcct ccacccccag ggcccgcagc acgaacgcca gcacgaaggc ctcgtcccgc
    13081 caggcgtcgt agcgccccga cgggccctgg tggcccgcgc ccatctcggt cttcaacaag
    13141 atcggcatcg ccccggtggc ccggtcgcgc agccgggcca cccatttcgc cggctcccag
    13201 tagctcaccc ggggatcgtt gaggccggcg gtgaccagca gggcggggta gtcctgggcc
    13261 gagacgttgt cgtagggggc gtaggacctc atgtaggcgt acacctccgg gtcggcgacc
    13321 gggttgcccc actcctccca ctccatcacc gtgagcggga gcgtgtcgtc caggatggtc
    13381 gtcaggcagt ccacgaacgg gacctcggcc acgatggcgc cgaacaggtc gggccgcagg
    13441 ttggccacgg cgcccatcag caggcctccg gcgctgccgc cccgggccgc cagccgcggc
    13501 ggcgaggtcc agcccccggc gaccagatgg tcggctgcgg cgacgaagtc ggtgaaggtg
    13561 ttccgcttgt ggagcatctt gccctgctcg taccacggcc ggcccatctc gccgccgccg
    13621 cgcacgtggg ccacggcgta cacgaacccc cggtcgagga ggcagagccg caccgaggag
    13681 aagccgggct cgaggctgtg ctcgtaggac ccgtagccgt acagcaggca gggcgccgac
    13741 ccgtcccggg ccaggccttc tctgtatacg agggaaatgg ggaccctcgc gccgtcgccc
    13801 gccgtggccc acacccgctc ggtgacgtac ggcgccgggt cgtagcccgg cacgggctgg
    13861 cgcttgagca gggtcgcgcg ccgggtgcgc aggtcgaagt cgatcaccga catcggcgtc
    13921 accatcgacg cgtactggta gcggagggtg gcggtctcga actccgggtt ggccccggcc
    13981 cacgtcccgt acaccgcctc cggctggtcg atgacgtagg acgacatgtc gtcgaggtcg
    14041 accacccgga tggtccgcag ccccgcggcg cgctcccaga ccacgaggtg gtgggcgaag
    14101 gcgtccacgt cctcgacccg gacctcgggg tcgtacggca gcagctcgcg ccagtgctcg
    14161 gggccgggag tggcctcggg cgcctccatg accgcgaagt tctcccgccc gcccgcgttg
    14221 gtcaccacga ggaaccggtc gccgtggtgg tcgatcgcgt actcgacccc gtgctggcgg
    14281 cgagcgacga cccggaagtc gccttcgggg tcgccggcgt cgagcacgcg cacctcggtc
    14341 gtgatcttgc tgccggcctg gagcacgacg aaggcgtcgt cccgggtccg cccgacctcg
    14401 aggaagaagt gctcgtcgtc ctcctggaac acgagcacgt cggccgtcgc gggcgtcccc
    14461 agtcggtgcc gccacacctg ccacggccgc atggcctcgt ccggcctggt gtagaagacg
    14521 gtcttgctgt cgttggccca cgccaccgag tagtaggtgt cctcgaccac gtcgtcgagg
    14581 tccgccccgg tgcccaggtc ccggaaccgc agggtgaagc gctcgccgcc gttcgtgtct
    14641 gcggcccagg cgaccagccc gtggccgggg ctgacctcga agttgcccag gtcgaagaag
    14701 tcgccgtcgc ccgcctctgc gttctcgtcg agcaggacgg cttcggcgcc ctctccctcc
    14761 gggcccggcg gcctccggca gtggatggcg tactggtggc cctcgatcgt gcgggcgtag
    14821 tacagccacg ggcccttgcg tgccgggacg gtcaggtcgg tctcctgcac gtggccgacg
    14881 atctcctcga acagctgcct ctgcaggtcc ttggtcccgg ccatctgggc cgcggtgaag
    14941 tcgttctcgg cttcgaggtg ggccgtgacc tcggggtcgt cccggtcgcg cagccagtac
    15001 cactcgtcga tccgctcgtc tccgtgggcg acgagggcgt gcgggcgctt ggcggcgcgg
    15061 ggcgggcgat cggtcacggc catgcttcta ccatcgaaaa ccatcaggcg cgccgccgcc
    15121 cttgaccggc gcccttgacc ggcgggtcaa gtagcatccg ggggccatgt ccacctctcc
    15181 gatggctgcc acgcgctcgg gcggggctct catgcgaacc accatcaccc ggtactggag
    15241 gggcgtcgtc atcggggccg gggccgggct ggtctggacg gcggccaagg tgtcgatccc
    15301 cgatctgatc ggacacgcca tcgacaaggg cgtcgtgcac ccgcagggcc acaccctgct
    15361 gacctacgcg ctcgtcatag ccgccgtcgg gatcgtccag ggcggctgca ccgggctgcg
    15421 ccgctactac gcgttctggg tcgcgtaccg ggtggagacc gacctgcgcc accgcctctt
    15481 cgcccacatc cagcggctgc acttagcgtt ccacgaccac gcccagaccg gccagctgat
    15541 gtcccgctcg gcgacggacc tccagcaggc ccagggcttc gtgtcgatga tcccgatcac
    15601 catctccagc gccgtcaccg tggtcgcggt ggccgccatc atgctggcca ccaacgtcaa
    15661 gctgacattt ctcgccctgt gcgccctgcc cctgatcaac ctggtggcca agacgttcag
    15721 ctcccgcgtc tatcccgtct cgctcggcct ccagcaggag ctggccggcg tggccaccgt
    15781 ggtcgaggag acggtgaccg gtatccgggc ggtcaagggg ttcggggccg aggccatcca
    15841 ggcccgggtg ctgcgccagc ggaccgatcg ggtgtggcac cgggccatgg ccgcggcccg
    15901 gatccgggcc acctacgcgc ccctgctcga cgtgctgccc gccgtgggca tggtcctggt
    15961 gctgtggtac ggcgggcacc aggtgctgtc ccgccacctg tcggtgggcc agctgacgga
    16021 gttcgtgctc tacgtcaccc tcctggtcaa cccgctgcgg atgctcggcc agctggtcgc
    16081 ccaggcccag cgcgccatcg cctccgccga ccgggttggt gagatccttt ccaccgagcc
    16141 ggagatcacc agcagcccca aggccccgaa gctgcccccc ggcggcgggg ccctgcgctt
    16201 cgacgacgtg cgcttcgcct accagcccgg cgccgagccg gtgctcgacg gcttcagcct
    16261 ggcggtcgag ccgggcgagt cggtcgcgct ggtcgggccg accggatcgg gcaagacgac
    16321 cgtggcccgg ctgatccccc gcttctacga catccagcac gggacgatca ccatcgacgg
    16381 cgccgacatc cgcaaggtgc ggctccccga cctgcgccgc aacgtcggca tcgtgttcga
    16441 ggacacgttc ctgttcaccg actccattcg ggccaacatc gccttcgccg agcccgatgc
    16501 ccctttcgac caggtcgtgc gggcggcccg ctcggccggc gccgacgagt tcatccgggc
    16561 catgcccgag ggctacgaca ccatcatcgg cgagcggggc ttctcgctgt cgggcggcca
    16621 gcgccagcgc atcgccctgg cccgggccat cgtcgcggac ccccgcgtgc tcatcctcga
    16681 cgacgccacc tcctcggtcg acccgaccaa ggagcacgag atccgtggag ccctcctgga
    16741 ggtcatgcgg ggccgcagca ccatcgtcat cgcccaccgt cccgcgacga tcgcactggc
    16801 cgaccgggtc atcctcctcg acgggggccg cgtcgtggcc cagggcaccc acgaggagct
    16861 gctcgaaacc agtgagcgct accgggaggt gctcgcccgg gccgagggca gccacaacgt
    16921 ggcggccggc ctctccgggg acgcgggctg atgggccgct ggagcaacgt cgccgtcgac
    16981 gaggaggaca agctcgaccg caagggcgcc acccgcgtca tgcggcgcag cttccggatg
    17041 ctgcgcaggt accggccgct ggtcgtcgcc tcggcgctgg tcatgatcgg ctacacggcc
    17101 ggactggtgg cgggccccgt cgccatccaa ttcggcatcg accagggctt gggcaagcac
    17161 agcggccacg acctggacct ggccgtcgtg ggctacgtgg ccgcctcggt gatcgtgctg
    17221 gtctgcgggc ggggccagat ccggctcgtc acccgcctcg gcgagaactt cctgcgagac
    17281 ctccgggtgc gcgtcttcga ccacatcctc gccctgtcca tggggttctt cgacaaggag
    17341 cagaccggca agctggtggc gcgcatgacc tccgacatcg acgccatgga ggacctcatc
    17401 cagctcggcc tggtccagtt cgtgaccaac gggctgctct tcgcggccac gatcggcgtc
    17461 atggtcgtcg agtcctggag gctgaccctc gtgtgcatgg tcagcgtccc cctggtggtg
    17521 gtcgagtcgc gcaagttccg gaggcggtcc aacgaggcct acctcaacgt ccgggaccgc
    17581 atcggccaga ccatgtccac catccaagag ggcatctccg gggtccgggt catccaggcc
    17641 ttcggccggg aggatctcca ggtcgagcgc ttctccaacc gcaaccaggc ccagctcgac
    17701 gccaacgtgc gggccgtggg catcagcgcc cgctacttcc cggtcatcga gttctcgggt
    17761 gtggcgacca tggcggcgct ggtcggcatc ggcggctacc tggtgcacac caaggccacg
    17821 acgctgggca ccgtcgcgtt gttcttcgtc ctgctccagc tgctgctcga cccgatcatc
    17881 cagctgtccc agctgttcaa cctggtccag tcctcgggtg cggccctgaa caagctgttc
    17941 ggcctgctgg acaccgactc ggaggtgaag gaacgcccag gggcggtgga cctcccggac
    18001 gggggcgcct tcgaggtgcg gggcgtgggc ttcgcctacg ggccggacct gccgccggtg
    18061 ctcgacggcg tcgatctcac cgtcgcctcc ggcgagcggc tcgccctggt ggggccgacc
    18121 ggcgccggca agtcgacgct ggccaagctc atctcccgct tctacgaccc gaccgagggc
    18181 gccgtcacct acggcggggt cgacctgcgc gacgccaccc tgggctcgct gcgccagcgc
    18241 atctgcgtgg tgccccagga gggcttcctg ttccacggga cgatcatgga caacgtccgg
    18301 ctgggccgcc agggcgcgac cgacgaggag gtccgggccg cgctcggcac catcgggatc
    18361 ggggaccgct tcgacgccct gccagagggt ttggagaccg aggtcaggga acggggctcc
    18421 cgcctgtcgg ccggcgagcg ccagctcgtg agcctggccc gggccgcgct cgccgacccc
    18481 caggtgctcg tcctggacga ggccacctcc aacctcgacc ccggcaccga ggtgatcgtc
    18541 gagggtgcgg tcaacgccct catgaccggc cggacggtga tcatcatcgc ccaccggctc
    18601 tccaccg
//
LOCUS       DASWGG010000371         4098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_518675, whole genome shotgun sequence.
ACCESSION   DASWGG010000371 DASWGG010000000
VERSION     DASWGG010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518675"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VF954_06790"
     CDS             <1..346
                     /locus_tag="VF954_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530825.1"
                     /translation="HVEHLTLSRVWHEASNFDPEIAEARRRERAHRVDRVRRGIEAAD
                     LRPGIDPTVAAAALTAMLEEFAVRWFDEGDGPGKSASDIVSAGETIATMWLTVIGLEH
                     RPAPSPPPSDKN"
     gene            565..936
                     /locus_tag="VF954_06795"
     CDS             565..936
                     /locus_tag="VF954_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530826.1"
                     /translation="MPMREDCRHFQSRTYDDGEVARLCTLNLAPEAPWRCPENCPEYR
                     RDVLTVGFTSGSLTRPPVEAEPDDPPGDIAGLLEQADDIVNSAGPAIVAEFEAERRSK
                     RWWQFWKKGDDGGNEHRMSNR"
     gene            complement(965..1780)
                     /locus_tag="VF954_06800"
     CDS             complement(965..1780)
                     /locus_tag="VF954_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9530827.1"
                     /translation="MAVWIDDPDDPRVADYVDLTDSQMRRRSEPGAGVFIVEGALTLA
                     ALARSRYAARSVMVTPSQAERLRDLLDGLGAPVLIASQPVMNRVTGFNIHRGVLAAAA
                     RPPLLSPDEVAGLARVLVVGEDLNDQENLGALFRNARALGVGGVLLSPGCCDPLYRRT
                     VRVSLGHVLHVPFAVLRPWPSGLLGLRAKGFEVLALTPGPDAEPIGAVDAAGAGLDRR
                     VAVLVGAEGAGLSPAALAYADRRVRIPMAPGVDSLNVATAAAIAFHHLGAAPT"
     gene            1846..2280
                     /locus_tag="VF954_06805"
     CDS             1846..2280
                     /locus_tag="VF954_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530828.1"
                     /translation="MHPTTRAWAAGVASTALLVIGVVGVVGVAVHHSRRAPTASPPPG
                     SAAPTTAAPTTAAPTTVGPTTASTATTTRPSGLAAGGSGHVASTPQPLADTGRRPGAD
                     AGVALGLLGAGALVLRRRSEPGQPARLPASAGSPIGLAHSDD"
     gene            complement(2430..2693)
                     /locus_tag="VF954_06810"
     CDS             complement(2430..2693)
                     /locus_tag="VF954_06810"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX9530829.1"
                     /translation="MCRSIVTLRGEEPASEDEIAAAALQFVRKVSGQRAPSRANQAVF
                     DRAVIEVAAATRRLLGDWEHPAGARQPALAPSRIVARARRTPG"
     gene            complement(2710..3432)
                     /locus_tag="VF954_06815"
     CDS             complement(2710..3432)
                     /locus_tag="VF954_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9530830.1"
                     /translation="MSRLTFLGTGNFLAPGRYWNSFVLDDDVLVEPAPTSLPNLRRAG
                     IAAGAIQTVVISHFHADHTFGWPFLLLELLRSRPAEALWVVGPPGVESHLADMMRVGS
                     VRGVHEDAHARMDIRYVEVDGTWQQAGMLRLRAVEVEHVPYLRCYGFLFDRGPRTLGY
                     SGDTRPCGGLDELAHEAHALVLECNGPHPPPPTHMEVPDVRALQARNPGLRLILTHLG
                     EGVEADVPDGAIVPSDFAVVEL"
     gene            complement(3449..>4098)
                     /locus_tag="VF954_06820"
     CDS             complement(3449..>4098)
                     /locus_tag="VF954_06820"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9530831.1"
                     /translation="AYVAPFTQDGRPLGHPDNDPVFAAAQALGVPFAIHPTFEPQWTK
                     GTRMGSWENVRELRLLGSVMASDGVRQQFTTLFDYGVFDRFPELKVVVLESGGGWIGY
                     WLDRIDAVYGHTFIGTKVPLKHKPSDYFRDRVWISCDPDERTIPALAERFGADRFMWA
                     SDFPHADHTPEYVGDLAELAASFDGAPRGRFLGDNARELYQIPDTAVCRQPAATP"
BASE COUNT          620 a         1467 c         1496 g          515 t
ORIGIN      
        1 ccacgtcgag cacctgaccc tgtccagggt gtggcacgag gcgtcgaact tcgatcccga
       61 gatcgccgag gcccggcgcc gcgaacgggc ccatcgcgtc gatcgggtcc ggcgagggat
      121 cgaggcggcc gacctgcggc cggggatcga ccccacggtc gcggccgccg ccctgaccgc
      181 catgctcgaa gagttcgcgg tgcgctggtt cgacgagggc gacggccccg gcaagagcgc
      241 cagcgacatc gtctcggcgg gcgagaccat cgccaccatg tggctcaccg tcatcggcct
      301 cgagcaccgc ccggccccga gccccccacc gtcggacaag aattgacggt gaccgaagcc
      361 gccgcggacg ggtgcgacgg taccggggac cgccccccgc ccggctgggg ggaggacagg
      421 caggagggcg gaaggtcccc ggccgatggg atcgaaccta ccggctgagt gtttcgggcc
      481 cgcgacggcc gcgaaaactg cgggtgaaag ggaccggcat ccggtcaggg cgtcatcgtg
      541 gcccggagtg ccagactggc aggaatgccg atgcgcgaag actgccgtca tttccagagc
      601 cgcacctacg atgacggcga ggtggcccgg ttgtgcaccc tcaacctggc tcccgaggcg
      661 ccgtggcgct gccccgagaa ctgccccgag taccggcgcg acgtgctgac cgtgggattc
      721 acgtccggct cgctcacccg tccgcccgtc gaggccgagc cggacgaccc gcccggggac
      781 atcgccggcc tgctcgagca agccgacgac atcgtgaact cggccgggcc cgccatcgtc
      841 gccgagttcg aagccgagcg ccggagcaag cggtggtggc agttctggaa gaaaggtgac
      901 gacgggggca acgagcaccg gatgagcaac cgctagccgc ctggctgctc ggcacggccc
      961 ttggtcacgt cggcgccgcg cctaggtggt ggaacgcgat cgcggccgcg gtcgccacgt
     1021 tgagcgagtc gacgcctggc gccatgggga tccgcacgcg ccggtcggca taggccagag
     1081 ctgccgggct caggccggcg ccctcagccc cgacgagcac cgccacccgc cggtccagac
     1141 ctgcgccggc tgcgtctacg gcgccgatcg gttcggcatc gggcccgggc gtgagcgcca
     1201 gcacctcgaa gcccttggcg cggagcccga ggagaccaga tggccaaggc cgcagcacgg
     1261 cgaagggcac gtggagcaca tggcccagcg agacccggac ggtccgtcgg tagagcggat
     1321 cgcagcatcc cggggacagc agcaccccgc ccacaccgag ggcgcgggcg ttgcggaaga
     1381 gggcgccaag gttctcctgg tcattgaggt cttcacctac caccaggacg cgggccaatc
     1441 ccgccacctc gtccggggag agcaggggcg ggcgggcagc ggccgccagc acgccgcggt
     1501 ggatgttgaa gccggtcacg cggttcatga ctggctggct ggcgatgagg acgggggcgc
     1561 ccaggccgtc cagcaggtca cggaggcgct cggcctgcga cggcgtgacc atgaccgacc
     1621 gggccgcgta ccgggagcgg gccagcgcag cgagggtcag ggcgccctcg acgatgaaca
     1681 cgcccgcgcc tggctccgaa cggcggcgca tctgcgaatc ggtcaggtcg acgtagtcgg
     1741 cgaccctcgg atcgtccggg tcgtcgatcc acacggccac ggcgccgcac cctaccgggt
     1801 cgctgaggtc cccgtaggcc catttgggcc attagcgtgc cgggcgtgca cccgaccacg
     1861 agagcctggg ccgcgggggt cgcgtccacc gccctcctcg tgatcggcgt ggtcggcgtg
     1921 gtcggcgtgg ccgtgcacca ctcccgccgg gcaccgacgg ccagcccgcc acccgggtcc
     1981 gctgcgccca cgaccgcggc gcccacgacg gccgcgccca ccaccgtggg gccgaccacc
     2041 gcgtccacgg cgaccaccac ccgaccgtcc ggtctggcgg ccggcggatc cggccacgtg
     2101 gcatccaccc cgcagccgct ggccgacacc ggacgccgcc cgggtgcaga cgcgggcgtg
     2161 gcgctcggac ttctcggcgc gggcgccctg gtcctgcgga ggcggtccga gccgggccag
     2221 cctgcccggc ttccagcctc ggcgggtagt ccgatcggac tagcccattc ggatgattga
     2281 cgtcaccggg ccgatcgtgg acactggagg catcccgccc cccgggagcc ccgagcaccg
     2341 ccggcccatg ccggcggtgc tccgcgtcgg gggccagggc tggtgcctgc caagcgaacg
     2401 gacccgccgg gcccgatcgg cctcaaccct cagcccggcg tacggcgcgc cctggcgacg
     2461 atccggctcg gggccaaggc cggctgacgg gccccggccg ggtgctccca atcccccagc
     2521 aaccggcggg tggccgcggc cacctcgatc acggcccggt cgaagacggc ttggttggcc
     2581 cgcgaagggg cccgctggcc gctcaccttg cgaacgaact ggagcgcggc cgcggcgatc
     2641 tcgtcctcgc tcgccggctc ctcaccacgc aatgtcacga tgcttcggca cattggccca
     2701 ttgtgcacgt cacagctcca cgacggcgaa gtcgctaggc acgatggcgc catccggcac
     2761 gtcggcctca acaccttcgc cgaggtgggt caggatcagc cgcagaccag ggttccgggc
     2821 ctgcagggcg cggacgtcgg gcacctccat gtgggtgggc ggcggcgggt gggggccgtt
     2881 gcactcgagg accagggcgt gcgcctcgtg ggccagctcg tcgagcccgc cgcacgggcg
     2941 tgtgtcgccc gagtaaccga gggtgcgggg gccccggtcg aagaggaagc cgtagcaccg
     3001 caggtagggc acgtgctcca cctcgacggc gcgcaagcgg agcatgccag cctgctgcca
     3061 ggtgccgtcg acctcgacgt agcggatgtc catccgcgca tgcgcatcct cgtggacccc
     3121 gcggacggag ccgacgcgca tcatgtccgc caggtggctc tccacgccgg gcggccctac
     3181 gacccacagg gcttcggccg gccggctgcg caggagctcg agcagcagga agggccagcc
     3241 gaaggtgtgg tcggcgtgaa aatgggagat gacgaccgtc tggatggccc cggcggcgat
     3301 gccggcgcgg cgcaggttgg gcagcgaggt cggggccggc tcgaccagca cgtcgtcatc
     3361 cagcacgaag ctgttccagt accggcccgg tgcgaggaag ttgccggtac ccaggaatgt
     3421 gagccggctc aaggtcgcgc ccggcacctc atggcgtggc ggctggctgg cggcataccg
     3481 ccgtgtccgg gatctggtac agctccctgg cgttgtcgcc caggaagcgg ccccggggcg
     3541 cgccgtcgaa ggacgccgcc agctccgcca ggtcgcctac gtactcggga gtgtgatcgg
     3601 cgtgcgggaa gtccgaggcc cacatgaacc ggtcggcgcc gaagcgctcc gccagggcgg
     3661 ggatggtccg ctcatccggg tcgcaggaga tccagacccg atcgcggaag tagtcgctgg
     3721 gcttgtgctt caacgggacc ttcgtgccaa tgaaggtgtg gccgtacacg gcgtcgatcc
     3781 ggtccagcca gtacccgatc cacccgccgc ccgattccaa gaccacgacc ttgagctcgg
     3841 ggaagcggtc gaagacgccg tagtcgaaca gggtcgtgaa ctgttggcgg accccgtcgg
     3901 atgccatcac cgaccccagc agccgcagct ccctgacgtt ctcccaggaa cccatccgcg
     3961 tgcccttcgt ccattggggc tcgaaggtcg gatggatggc gaagggcacg cccagggcct
     4021 gggcggcggc gaagacaggg tcgttgtccg ggtggccgag gggccggccg tcctgggtga
     4081 acggcgccac gtacgcgc
//
LOCUS       DASWGG010000372         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_196150, whole genome shotgun sequence.
ACCESSION   DASWGG010000372 DASWGG010000000
VERSION     DASWGG010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196150"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..89)
                     /locus_tag="VF954_06825"
     tRNA            complement(16..89)
                     /locus_tag="VF954_06825"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Gln,seq:ttg)
     gene            159..1256
                     /locus_tag="VF954_06830"
     CDS             159..1256
                     /locus_tag="VF954_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX9530832.1"
                     /translation="MTSRPLSALVLAAGEGTRMRSSRPKPLHLLCGRPMVLHVLDALA
                     ELPVDRVVVVVGHGAERVVKAVQDQAPSTFGIEFVEQHTQRGTGDAVAVGLTGFEDDI
                     EDGDLVVLPGDTPLLRPPTLAALVRLHRHADAAATLLTAVLDQPAGYGRIVRDRNERV
                     AGIVEEADATAEEQAITEVCTSIYCFRKSVLAPALRRLSPENANGEYYLTDAIGVLHD
                     AGYAVTSLVAGDPMEAAGVNDRAQLAIAEAELRDRTNERWMRRGVTMLDPERTYIDLS
                     VELGTDVTLFPGTMLQGATRIGPGAQIGPDTRLVDCSVGPRARVAQTSARLSDIGADA
                     VVGPFAALEPGYRVADGAVTGPFFRGVDDGG"
     gene            1262..2233
                     /locus_tag="VF954_06835"
     CDS             1262..2233
                     /locus_tag="VF954_06835"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX9530833.1"
                     /translation="MELVPKKRLVLYSGRAHLELAEEIAAALGTTLGEANLKSFPIGE
                     IHCRFDESIRGADVFIVQTHCSPVNDAIMEQLIMIDAAKRASAKRITAVCPYYGYARQ
                     DRKVSGREPIAAKLVADLLRTAGADRIMSVDLHSGQIQGFFDGPFDHLVAMPLLLEYL
                     RDHAGPEAVIVAPDAGRVKVADRFAEHLDLDLAIVQKRRPKNTTGTVQVVSVVGEVAG
                     RHCVLIDDMIDTAGTVCAAAERLADEGATDVWAAATHAVLSDPATDRIKNSSISRLIV
                     TNTLPIPPEHRLDKLEVLSVASIIANAIDAVFEDASVSEIFGGENQA"
     gene            2323..>2983
                     /locus_tag="VF954_06840"
     CDS             2323..>2983
                     /locus_tag="VF954_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX9530834.1"
                     /translation="MPEIPVIAEVGRPTGSASSRRLRHHGKVPAVLYGHGMEPQPLAV
                     DGRSLRAALTTQAGLNALLSLQVDGASHLAMARDIQRDPVRRTVSHVDFQVVRRDEVM
                     AADVPIVLVGEATEVAIGDGLVDQQMHTLSIRATPDAIPNVIEVDVSGLAIGETIRVG
                     DLELPARVTTEADPEQAVVVGQPPQVLAADLVAEAEAVEGEAAAGQPVAASGPAGDGE
                     AG"
BASE COUNT          441 a         1091 c         1052 g          399 t
ORIGIN      
        1 gctccggagg aggggctccg gggggagggc tcgaacctcc aagcatccgg ctccaaaggc
       61 cggcgttcta ccgattgaac taccccggac cgaccgccca acggtactcg tgcccgagat
      121 gccaccgcgg ccgccgcggg cgggtagaca atggagccat gacgtcccgg cctctgtctg
      181 cactggtgct ggccgccggc gaagggaccc gcatgcggtc ctcccgcccc aagcccctcc
      241 atctcctctg tggccgcccc atggtcctcc acgtcctcga cgccctggcc gagctgcccg
      301 tcgaccgcgt cgtggtggtg gtgggccacg gcgccgagcg ggtcgtgaag gcggtccagg
      361 accaggcccc gtccacattc ggcatcgagt tcgtcgagca acacacccag cgaggcaccg
      421 gcgacgcggt cgcggtcggc ctcaccggct tcgaggacga catcgaggac ggcgacctcg
      481 tcgtgctccc tggcgacacg ccgctgctgc ggcccccgac gctcgcggcc ctggtgcgcc
      541 tccaccgcca cgcggacgcg gcggccaccc tcctcaccgc cgtcctcgac cagccggcgg
      601 gctacgggcg catcgtgcgg gaccgcaacg agcgggtggc cggcatcgtc gaggaggccg
      661 acgccaccgc cgaggagcag gccatcacgg aggtctgcac ctcgatctac tgcttccgca
      721 agagcgtgct ggcacctgcc ctgcgccgct tgagcccgga gaacgccaac ggtgagtact
      781 acctcaccga cgccatcggg gtgctccacg acgccggcta cgcggtgacg agcctggtgg
      841 cgggcgatcc gatggaggcg gccggcgtga acgaccgggc ccagctggcc atcgccgagg
      901 ccgagctgcg ggaccgcacc aacgagcggt ggatgcgccg gggggtgacc atgctcgacc
      961 ccgagcggac ctacatcgac ctgtccgtcg agctcggcac ggacgtcacg ctgttccccg
     1021 gcaccatgct ccagggggcc acccggatcg ggccgggtgc gcagatcggg cccgacaccc
     1081 ggctcgtcga ctgctcggtg gggccccggg cgagggtggc gcagacctcg gcccggctct
     1141 ccgacatcgg cgccgacgcg gtggtgggac ccttcgccgc cctggagccc ggatatcgag
     1201 tggcggacgg ggccgtcacc ggtccattct tccgtggggt cgacgacggg ggttgatgtc
     1261 catggagctg gtgcccaaga agcgactggt cctctactcg ggccgcgccc acctcgagct
     1321 ggccgaggag atcgcggccg ccctgggcac caccctgggc gaggccaacc tcaagagctt
     1381 cccgatcggc gagatccact gccgcttcga cgagtccatc cggggcgccg acgtgttcat
     1441 cgtgcagacc cattgctccc cggtgaacga cgcgatcatg gagcagctca tcatgatcga
     1501 cgccgccaag cgggcctcgg ccaagcggat cacggccgtg tgcccgtact acggctatgc
     1561 ccggcaggac cgcaaggtgt cggggcgaga gcccatcgcg gccaagctgg tggccgatct
     1621 cctccgcacg gccggggcgg accgcatcat gagcgtcgac ctgcattcgg gccagatcca
     1681 gggcttcttc gacgggccgt tcgaccacct ggtagcgatg ccgctgctcc tggaatactt
     1741 acgggaccac gccgggcccg aggcggtgat cgtggccccc gacgccggcc gggtgaaggt
     1801 cgccgaccgc ttcgcagagc acctggacct cgacctcgcc atcgtccaga agcgccggcc
     1861 caagaacacg acggggacgg tccaggtggt ctctgtcgtg ggggaggtag cggggcgcca
     1921 ctgcgtgctg atcgacgaca tgatcgacac ggcggggacg gtgtgcgcgg ccgccgagcg
     1981 cctggccgac gagggcgcga ccgacgtctg ggccgcggcc acccatgccg tgctgtccga
     2041 ccccgccacc gaccgcatca agaactcgtc gatctcgcgg ctgattgtca ccaacaccct
     2101 gccgatcccg ccggagcacc gcctggacaa gctcgaggtc ctctcggtgg cgagcatcat
     2161 cgccaacgcc atcgacgccg tgttcgaaga cgcgtcggtg tcggagatct tcggggggga
     2221 gaaccaggcc tagttgaggg cacggccgcc ggccgggccc aatgggcacg gcgcgcccgg
     2281 cgactacact cggccggtcc cacccagccg ttggagcgcg ccatgcccga gatccccgtc
     2341 atcgccgaag tcggtcgccc gaccggctcc gcgtcctccc gccggctgcg ccaccacggc
     2401 aaggtgccgg cggtcctgta cggccacggc atggagcccc agcccctcgc ggtggacggc
     2461 cgcagcctcc gggccgcgct gacgacccag gcgggcctca acgccctgct gtcgctccag
     2521 gtggacggcg cctcccacct ggccatggcg cgggacatcc agcgcgaccc ggtgcggcgc
     2581 accgtgtcgc acgtggattt ccaggtggtg cgccgcgacg aggtcatggc cgccgacgtc
     2641 ccgatcgtgc tcgtgggcga ggccaccgag gtcgccatcg gggacggcct ggtcgaccaa
     2701 cagatgcaca ccctgagcat ccgggctacg ccggatgcga taccgaacgt catcgaggtg
     2761 gacgtgtccg gcctggccat cggcgagacc atccgggtgg gtgacctcga gctgcccgct
     2821 cgggtgacca cggaggccga cccggagcag gccgtcgtgg tcggccagcc gccccaggtg
     2881 ctggccgccg acctggtggc tgaggcggaa gcggtggaag gcgaggcggc tgccggccaa
     2941 ccggtcgcgg cctcgggacc ggccggtgac ggggaggcgg gcg
//
LOCUS       DASWGG010000373         3968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2429072, whole genome shotgun sequence.
ACCESSION   DASWGG010000373 DASWGG010000000
VERSION     DASWGG010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2429072"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2456
                     /locus_tag="VF954_06845"
     CDS             <1..2456
                     /locus_tag="VF954_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9530835.1"
                     /translation="MCRMCVVEVKGPRGFALLPACYVRARDAAEVVTTSPKVKKAQDG
                     ILELLLINHPLDCPVCDKGGECPLQDQTLAFGPGESRFVEEKRHWEKPIALSSLVHLD
                     RERCIQCDRCTRFALEVAGDPLITFQQRGNRTEINTFPGQPFSSYFSGNTVQICPVGA
                     LTAAPYRFRARPWDLEQVESTCTSCAVGCRVAVQSTSNRLARYLGIDSEPVNHGWLCD
                     KGRFAFEYVNSEERLAGPLVRKGDELVPASWSEALQAASQGLRHAVSVHGPASVAVIG
                     GARLANEDAFAWAKLAKSVLGTDSVDAQLGDGLPAEAVLGLPRATIDQACGAKAVVLL
                     APDLKEELPVLYLRLRAAAVDRGLRIIEMAPASTGMTRYSEVTLAYRPGEAPALARAL
                     TAGGPAAGGPAAAGGEPDAAPPAEPAPAAVAGVPASAIAAAREVLAGAEGDVVVVLGR
                     TSLAESGQSVADAAAVLASGLPSARFLPALRRANVHGALDMGLAPGLLPGRVALDAGR
                     EWYEQAWGPLPAETGRGTAEILAAASEGRVQGLILLGADPLADFPDRDLARRALLATG
                     LVVSVDTFVNDSNRGADVVLAAAAYSERGGTTTNLEGRITRLGQKIVPPGVAWPDWMI
                     AVELAHRLGGDLGFETIEQIWDEIERVAPSHAGITHALLASPLARDGVVAPLSPETAS
                     TTLPGAGGPPRPPALAFAPDIEKVAVPSLDAYSLRLVAHRSLYDDGVLTARSPSLDGL
                     SPPVRLRANPYDLDRLGVTTGGRVRVSSPRGAFSIEVIADPGVGRGSAALAFNLGRES
                     AGALIDAGQLVTDIRLETI"
     gene            2456..3682
                     /gene="nuoH"
                     /locus_tag="VF954_06850"
     CDS             2456..3682
                     /gene="nuoH"
                     /locus_tag="VF954_06850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX9530836.1"
                     /translation="MGDPLFSGGHVTLAVFVIVVVKVVVAFVALLVSVLLMIWFERKV
                     IADMQNRIGPNRAGPYGILQSVADGVKLFMKEDLLPDRADRRIFRLAPYLSAVPAFVA
                     FAIVPVGPDVTVAHHTFELQVADPPIGILFLLAMSSVAVYGVMLAGWSSGSKYPLLGS
                     VRASAQMVSYEAAMALSVAAVVLETNSLSTRDIVLQQAAHWNVVGLPVPTWNILRLGF
                     VPFVIFVIAGTAELNRPPFDLVEAEQELVGGFNTEYSSARFAMFYLAEFMNTITMSAI
                     IVTLFLGGPDGPWFHSVAGSRHTFPAVPWVGGLLWFFGKLLVFLFCYVWLRATLPRFR
                     YDQLMDLGWKRLIPGALVWLLVLAAMLINFRYGLAVVGGAVVFGLLLARSMKVGRSRA
                     TDAGPNRPPSLHRAGG"
     gene            3690..>3968
                     /locus_tag="VF954_06855"
     CDS             3690..>3968
                     /locus_tag="VF954_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9530837.1"
                     /translation="MGYLDGFKVTLKQMFEPEVTVSYPADKRPKPARFHGRHVLNRYE
                     DGMEKCIGCELCAGVCPAKCIYVRGADNPPDAPVSPGERYGFVYEINYL"
BASE COUNT          482 a         1495 c         1357 g          634 t
ORIGIN      
        1 ggatgtgccg catgtgcgtc gtggaggtca aggggccccg cgggttcgcc ctcctgcccg
       61 cgtgctacgt ccgggcccgg gacgcggccg aggtcgtcac cacctcgccc aaggtcaaga
      121 aggcccagga cgggatcctc gagctcctgc tcatcaacca tcccctcgac tgcccggttt
      181 gcgacaaggg cggcgagtgc ccgcttcagg accagacgct ggcgttcggc cccggcgagt
      241 cgcggttcgt ggaggagaag cgccactggg agaagcccat cgcgctgtcc agcctggtcc
      301 acctggaccg cgagcggtgc atccagtgcg accggtgcac ccgcttcgcc ctcgaggtgg
      361 ccggcgaccc gttgatcacc ttccagcagc ggggcaaccg gaccgagatc aacaccttcc
      421 cgggccagcc gttctcgtcg tacttcagcg gcaacaccgt gcagatctgc ccggtgggtg
      481 cgctcaccgc cgcgccgtac cgcttccggg cccggccgtg ggacctggag caggtggaga
      541 gcacctgcac ctcgtgcgcc gtggggtgcc gggtcgctgt ccagtccacg tccaaccgtc
      601 tggcccgcta tctgggcatc gacagcgagc ccgtcaacca cggctggctg tgcgacaagg
      661 gccgcttcgc cttcgagtac gtcaacagcg aggagcgcct ggccgggccg ctcgtccgga
      721 agggcgacga gctcgtgccg gcctcctgga gcgaggcgct ccaggccgcc agccaggggc
      781 tgcgccatgc cgtcagcgtc cacggaccgg catcggtggc cgtcatcggc ggcgcccggc
      841 tggccaacga ggacgccttc gcgtgggcca agctggccaa gagcgtgctc gggaccgact
      901 ctgtcgacgc ccagctgggc gacggccttc ccgccgaggc cgtgctcggt ctgcctcgcg
      961 ccaccatcga ccaggcttgc ggcgccaagg ccgtggtcct gctcgcgccc gacctcaagg
     1021 aggagctccc ggtcctgtac ctcaggctgc gcgccgcagc cgtcgaccgg ggcctgcgga
     1081 tcatcgagat ggcgccggcc tccaccggga tgacccggta cagcgaggtc accctggcct
     1141 accggccggg cgaggcgccc gccctggccc gggccctgac cgccggcggg ccggcggctg
     1201 gtgggccggc ggccgctggt ggggagcccg acgccgcccc tcccgccgag cccgcgccag
     1261 ccgccgtcgc gggcgtgccg gcctcggcca tcgcggccgc ccgcgaggtt ctcgccggcg
     1321 ccgaaggcga cgtggtggtg gtgctgggcc gcacctcgct ggccgagtcg ggccagtccg
     1381 tcgccgatgc cgccgccgtg ctcgccagtg gtctcccgtc cgcccggttc ctgccggcgc
     1441 tgcggcgggc caacgtgcac ggcgcgctcg acatgggcct ggcgcccggg ctgctccccg
     1501 ggcgggtggc cctcgacgcc gggcgggagt ggtacgagca ggcatggggc ccgctgccgg
     1561 ccgagaccgg gaggggcacc gccgagatcc tggccgccgc gtccgagggc cgggtccagg
     1621 gcctgatcct cctcggggcc gatcccctgg ccgacttccc cgaccgggac ctggcccgcc
     1681 gggccctcct cgccaccgga cttgtcgtgt cggtcgacac cttcgtcaac gactccaacc
     1741 gcggcgccga cgtcgtgctg gcggcggcgg cgtactccga gcgcggtggc accacgacca
     1801 acctcgaggg ccgcatcacc cgcctgggcc agaagatcgt gcctcccggt gtggcctggc
     1861 ccgactggat gatcgccgtc gagctggccc accgactggg cggcgatctc ggcttcgaga
     1921 ccatcgagca gatctgggac gagatcgagc gggtggcgcc gtcccacgcc ggcatcaccc
     1981 atgccctcct cgcctctccg ctggcccgcg acggcgtggt ggcgcccctc tccccggaga
     2041 cggcgtcgac gaccctgccg ggggccggcg gccccccgag gccgcccgcc ctcgccttcg
     2101 cgcccgacat cgagaaggtg gccgtgccga gcctggatgc ctactcgctg cgcctggtgg
     2161 cccaccgctc cctgtacgac gacggggtgc tgacggcgcg ctcaccctcg ctcgacggcc
     2221 tgtccccgcc cgtgcggctg cgggccaacc cctacgatct ggaccgcctg ggtgtcacca
     2281 cgggaggccg tgtgcgcgtc agctcccctc ggggcgcctt ttccatcgaa gtgatcgccg
     2341 atcccggggt gggccgcggc tcggccgccc tcgccttcaa cctggggcgg gagagcgccg
     2401 gcgccctcat cgacgccgga cagctggtga ccgacatccg gctggagacg atctgatggg
     2461 cgacccgctc ttctccggcg gccacgtgac gctcgccgtc ttcgtcatcg tggtggtcaa
     2521 ggtggtggtg gccttcgtcg ccctcctcgt gtcggtgctg ctgatgatct ggttcgagcg
     2581 caaggtcatc gccgacatgc agaaccgcat cggccccaac cgggcgggcc cctacggcat
     2641 cctccagtcg gtggccgacg gcgtcaagct gttcatgaag gaggacctgc ttcccgaccg
     2701 ggccgaccgg cgcatcttcc ggctggcccc gtacctgtcg gccgtgcccg ccttcgtggc
     2761 cttcgccatc gtgcccgtcg ggcccgacgt cacggtcgcc caccacacct tcgagctgca
     2821 ggtggccgac ccgccgatcg gcatcctgtt cctcctggct atgtcgtcgg tggcggtcta
     2881 cggcgtgatg ctggcgggct ggtcctcggg gtcgaagtac ccgctgctcg gctcggtgcg
     2941 ggcctcggcc cagatggtct cctacgaggc ggccatggcc ctgtcggtgg cggccgtggt
     3001 cctcgagacc aactcgctgt cgacccgcga catcgtgttg cagcaggccg cccactggaa
     3061 cgtcgtcggc ctgcccgtcc cgacctggaa catcctgcgc ctcggcttcg tgcccttcgt
     3121 catcttcgtc atcgccggca cggccgagct caaccgcccg ccgttcgacc tggtcgaggc
     3181 ggagcaggag ctggtcggtg ggttcaacac tgagtactcg tcggcccgct tcgccatgtt
     3241 ctacctggcg gagttcatga acaccatcac gatgtccgcc atcatcgtca ccctcttcct
     3301 cggcggcccg gacgggccct ggttccactc cgtcgccggc tcgcgccaca ccttcccggc
     3361 cgtgccgtgg gttggcgggc tgctgtggtt cttcggcaag ctgctcgtgt tcctcttctg
     3421 ctacgtgtgg ctccgcgcca ccctcccgcg cttccgttac gaccagctga tggacctggg
     3481 ctggaaacgg ctcatccccg gcgccctcgt ctggctgctc gtcctggccg ccatgctcat
     3541 caacttccgc tacggcctgg cggtggtcgg cggcgccgtg gtgttcgggc tgttgctggc
     3601 gcgcagcatg aaggtcgggc gttcccgggc caccgacgcg gggcccaacc ggcccccctc
     3661 actgcaccgg gccggggggt gagccgctga tgggctacct cgacgggttc aaggtcaccc
     3721 tcaagcagat gttcgagccc gaggtcacgg tctcgtaccc cgccgacaag cgccccaagc
     3781 cggcccgctt ccacggtcgc cacgtcctca accgctatga ggacggcatg gagaagtgca
     3841 tcggctgtga gctgtgcgcc ggcgtgtgcc cggccaagtg catctacgtg agaggtgcgg
     3901 acaatcctcc ggacgcgccg gtctcgccag gcgagcgcta cggcttcgtg tacgagatca
     3961 actacctg
//
LOCUS       DASWGG010000374         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_201739, whole genome shotgun sequence.
ACCESSION   DASWGG010000374 DASWGG010000000
VERSION     DASWGG010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201739"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..681)
                     /locus_tag="VF954_06860"
     CDS             complement(22..681)
                     /locus_tag="VF954_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX9530838.1"
                     /translation="MFVKICGTTSEEDALLAVAMGADAVGFIFAASPRMIAATKARDI
                     VKRLPPEILTVGVFRNEAPQRVVETVHMAGLKAAQLHGRETPVQSRWIKQRVPVVIQA
                     FAAGDPTVRRAKEYGVDAVLLDHPTPGSGHVFDWTLAGEAPTGTRVIVAGGLTPDNVG
                     AAVAAVQPWGVDVVSGVERSPGHKDPAKLRAFVAAARAAAVAPYQGNEDARPYDWEEE
                     I"
     gene            complement(685..1467)
                     /locus_tag="VF954_06865"
     CDS             complement(685..1467)
                     /locus_tag="VF954_06865"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX9530839.1"
                     /translation="MATYLDRIVAAHRAGAAADQRSVGQLIEQARQCAAPVRGFAASI
                     SGRSDRLAVIAEVKRRSPSKGDLAPDLIPEVLAKAYADGGAACLSVLTDREFFGARPG
                     DLPEARATTDLPVLRKDFTVGEADVCDARLMGADAVLLIAATLSPSLCRDLLALAEEL
                     GLDGLVEIHDEAELELALDVGATLIGVNQRDLVTFEVDTRRAERLAEAIPAGAVRVAE
                     SGIGGPDDARRLADAGYHAILVGESLVTSPDPAADVRALRRV"
BASE COUNT          256 a          618 c          593 g          247 t
ORIGIN      
        1 cgcccgccgg accaggcggg ttcaaatctc ttcctcccag tcgtagggcc gggcgtcctc
       61 gttgccctgg tagggcgcga cggccgcggc tcgggccgcg gccacgaagg cccgcaactt
      121 ggccgggtcc ttgtggcccg ggctccgctc gacaccgctc accacgtcca cgccccaggg
      181 ctgcaccgcc gccacggccg cccccacgtt gtccggcgtc aacccgcccg caacgatcac
      241 gcgcgtgccg gtcggcgcct cgcccgcgag cgtccagtcg aacacgtggc ccgagccagg
      301 cgtggggtgg tccagcagca ccgcgtcaac gccatactcc ttggcccgcc ggaccgtggg
      361 atcgcccgcc gcaaaagcct ggatcaccac cgggactcgc tgcttgatcc accgggactg
      421 gaccggcgtc tcccggccat ggagctgggc cgccttcaag ccggccatat ggacggtctc
      481 gacgacccgt tgcggcgcct cgttgcgaaa cacgccgacc gtcaggatct cgggcggcag
      541 gcgcttgacg atgtcccggg cctttgtcgc tgcgatcatg cgcggtgacg ccgcgaagat
      601 gaagccgacg gcgtcggcgc ccatggccac cgccagcagc gcgtcctcct cgctcgtcgt
      661 gccgcagatc ttgacgaaca ccggtcagac tctccgcagc gcccggacgt cggcggccgg
      721 gtcgggcgag gtcacgagcg actcgccgac caggatggcg tggtagccgg cgtcggcgag
      781 gcgccgggcg tcgtccggtc ccccgatgcc cgactcggcc acccggacgg cgccggcggg
      841 gatggcctcc gccaggcgct cggcccgccg ggtgtcgact tcgaaggtga cgaggtcacg
      901 ctggttgacg ccgatgaggg tggcgcccac gtcgagcgca agctccagtt cggcctcgtc
      961 gtggatctcg accagcccat cgagtccgag ctcttcggcc agcgccagca gatcccggca
     1021 cagagacggg gaaagggtcg ccgcgatgag gagcaccgcg tcggcaccca tgagccgggc
     1081 gtcacacacg tcggcctcgc cgacggtgaa atccttcctc aacacgggga gatccgtcgt
     1141 ggccctggcc tcgggcaggt cgccagggcg cgccccgaag aactcccgat cggtgaggac
     1201 cgaaaggcag gccgccccgc cgtcggcata cgccttggcg agaacctcgg ggatgagatc
     1261 gggggccagg tcgcccttgg acggcgaccg gcgcttcact tcggcgatga cggcgagtcg
     1321 atcgctgcgg ccgctgatgc tggccgcgaa cccccgcacg ggagccgcgc actggcgggc
     1381 ttgctcgatc aactggccga ccgaccgctg atcggccgca gccccggccc gatgggcagc
     1441 gacgatccgg tccaggtagg tggccatgtc tccgccgagc ctatcgggcc aatgctgatg
     1501 tgccgatgct gatgtggcgc tcacacagtc ctcatactta gctgccaagc tgattcagtg
     1561 tggcagacgg tttaggcacc atcgtggtcg tcgaggacga cccgaatatc gccgatctgg
     1621 tcgacatgta cctccggcgg gagggatttc gggtcatcca ggccggcgac ggcgaggccg
     1681 ggctggccgc cgtcgatcgg gagaagccca ggct
//
LOCUS       DASWGG010000375         5967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_535312, whole genome shotgun sequence.
ACCESSION   DASWGG010000375 DASWGG010000000
VERSION     DASWGG010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535312"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VF954_06870"
     CDS             complement(<1..816)
                     /locus_tag="VF954_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9530840.1"
                     /translation="MLKRLLSSHLAPYRRPLLGIVALQAVQTTAVLTLPTLNARIIDR
                     GIMRGDLAYIWRMGGVMVAFSLVQIIFSVGAVYFGAKVAMSFGRDLRNNLFHQVTDFS
                     TREVGAFGAPSLITRITNDVQQIQLLVVMTCTMAIAAPVTMVIGVVMALRQDVGLSAV
                     LLVSMPAAAIVLGFLVARMVPAFRLMQERIDQINRVLREQITGIRVVRAFVREPYETK
                     RFESANQELTDVSLQAGRLMASMFPTVNVIINLSSAAVLWIGANRVGAGHLHVG"
     gene            complement(810..1406)
                     /locus_tag="VF954_06875"
     CDS             complement(810..1406)
                     /locus_tag="VF954_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9530841.1"
                     /translation="MGTVGTVVAPHPGKRASALPPDERRQMVVDATLSLLLQNGEMVT
                     TRQIADAAGIAEGTIFRVFEDKDALIDAVLDRALDREPLERALRTIDPGQPLERSLEA
                     AVVTLQQRVVDFWRLLTSIGTRFHDRHRPPQEVSAALVELLTAYRDQLAVEPLAAARM
                     LGALTLSVTHPVLVDEPMSPPELVRLFLYGTHAPEPSC"
     gene            1541..2968
                     /locus_tag="VF954_06880"
     CDS             1541..2968
                     /locus_tag="VF954_06880"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX9530842.1"
                     /translation="MSRLSPFAGRVAVCGVVLAAGLVPLAARSASAGGAVVAIAARPQ
                     GDGFWTATSDGGVLPSGAAPNLGSMAGRALAAPIVGLASTVDGNGYWLLGRDGGIFSF
                     GDAAFYGSTGGMRLNQPVVGMAATPTGQGYWFVAADGGIFSFGDAAFYGSTGGLRLNQ
                     PIAGMAAAPTGRGYWLVARDGGIFAFGNAPFYGSTGSLQLGAPVVGMTATSDGSGYWF
                     VASDGGIFAYGDAPFAGSAAGAATSGPIVGMAAHPGGGYWLAEASGADFPYGVSPVPG
                     GATQTSCGYVPPTPAYNTSYGFERDPNNGATMRWDPRTTIHYVIDPNGAPTDVEGVVQ
                     SAVGTAAQVSGLQFAYDGTYDSAADPGPAGSPLRFRWANLGAGTEGGRADYSWVYTPG
                     GNNELIAAVVNVNTASSLAPDYSNGASEGTLVLHELGHALGLGHASDSSQIMNAVLSP
                     SGPTGYGPGDRDGLWHQGPAAGCLN"
     gene            3193..4275
                     /locus_tag="VF954_06885"
     CDS             3193..4275
                     /locus_tag="VF954_06885"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX9530843.1"
                     /translation="MPPVVDPSNVYSQAGANLLTGAARSALPRVYVPDHGSNNVTVID
                     PATRRVLAVLPVGANPQHVVPSYDLSTLWVANNADGTNLGSLTPIDPTTGRAGPAVAV
                     DDPYNLYFTPDGAHAIVVAEARKRLDFRDPRTMRLQYSIDVRSCPGINHMDFSLDGSY
                     LIATCEDAGRLVKVDWTRRSVVATLELGTAALPQDIRLAPDGRLFYVADMMGNRLILV
                     DGDRLLIAGSVDLAASGGIGAHGLYPSRDARVLYVANRGSAGRVGVAPHGPGSVSVVD
                     FATRQVVANWPVPGGGSPDMGGVSADGTQLWLSGRYDNEVYVFDTRAGRLADRITVGP
                     AGREPHGLCVWPQPGRYSLGHTGNLR"
     gene            complement(4315..4677)
                     /locus_tag="VF954_06890"
     CDS             complement(4315..4677)
                     /locus_tag="VF954_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9530844.1"
                     /translation="MALEWHGSDRAARVVVIDDDEDFRLFVETMLQAIDGFTVVGRTR
                     PDIVVLDLQMPVVDGMTALPELRRLVPEARIVVVSAFPDLVTLIDVLDRGADLYLDKG
                     RAWEEIVPALIAAAEASA"
     gene            4805..5182
                     /locus_tag="VF954_06895"
     CDS             4805..5182
                     /locus_tag="VF954_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9530845.1"
                     /translation="MTSEPTTVLVVDDEPLVVRLVELNLEMDGYRVVAAYDGPSGLRA
                     AGEEHPDVIVLDMMMPGMDGLEVARHLKSDPGTATIPIIFLSARSSPADVAAGLAAGA
                     AVYMAKPFDAEELSAKIAELLKG"
     gene            5194..>5967
                     /locus_tag="VF954_06900"
     CDS             5194..>5967
                     /locus_tag="VF954_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530846.1"
                     /translation="MARWASVARVGLTGLVLAAAGGALIASAAAADRRVDRARAAARA
                     DAVRGAGQAVATGLASAIAQAHGVGAAIHLGPTPAQSQDLAGALAGADLLDDGAYVVD
                     ARGAVVVTTVARADLAHLLRTWPELDVALHGASISGLYEDPLEHTVDLLVAAPALPGT
                     AIVGRTRVPGGNLARAVSQIPIPSGTQLLVVDGHNELVNGLAVSPPGVEVATGLRAMV
                     QAGRTGAAGLGTARAEGGAALAVAHAPAGNGWVVLITQPA"
BASE COUNT          829 a         2177 c         2143 g          818 t
ORIGIN      
        1 gccgacgtgc aggtggccgg caccgacgcg gttggctccg atccagagca cggccgcgct
       61 ggagaggttg atgatcacgt tgaccgtcgg gaacatcgag gccatcaacc ggccggcctg
      121 cagcgagacg tcggtcagct cctggttggc ggactcgaag cgcttggtct cgtagggctc
      181 gcggacgaag gcccgcacga cacggatgcc ggtgatctgc tcgcgcagga cgcggttgat
      241 ctggtcgatg cgctcctgca tgagccggaa ggcgggcacc atccgggcca cgaggaagcc
      301 gagcacgatg gcggccgccg gcatgctcac gaggagcacg gcggacaggc cgacatcctg
      361 tcgcagggcc atgaccacgc cgatgaccat ggtcacggga gcggcgatgg ccatcgtgca
      421 ggtcatgacc accagcagct ggatctgctg gacgtcgttg gtgatgcgcg tgatcagcga
      481 cggcgccccg aacgccccca cctcgcgcgt ggagaagtcg gtcacctggt ggaacaggtt
      541 gttgcgcagg tcccggccga agctcatcgc caccttggct ccgaagtaga cggcgccgac
      601 ggagaagatg atctggacca gggagaaggc gaccatcacg ccgcccatcc gccagatgta
      661 ggcgaggtcg ccccgcatga tgccgcggtc gatgatgcgc gcgttgagcg taggcagcgt
      721 gaggaccgcc gtggtctgga ccgcctgcaa ggcgacgatg cccagcagcg gccggcggta
      781 cggggccagg tgagagctca ggagccgctt cagcacgagg gctccggggc gtgggtgccg
      841 tacaggaaca gcctcacgag ctcggggggc gacatgggct cgtccacgag gacgggatgg
      901 gtcaccgaca gggtgagggc gcccagcatg cgggccgccg ccaggggctc gacggcgagc
      961 tggtcccggt aggccgtcag cagctcgacc agggcggcgc tgacctcttg gggcgggcgg
     1021 tgccggtcgt ggaagcgggt cccgatgctc gtcaacagcc tccagaagtc cacgacgcgc
     1081 tgctgcaggg tgacgacggc cgcctccagg ctgcgctcga ggggctgccc ggggtcgatg
     1141 gtgcgcaggg cgcgctctag cggctcgcga tcgagggccc ggtcgagcac ggcgtcgatg
     1201 agggcgtcct tgtcctcgaa gacccggaag atcgtgccct cggcaatgcc tgcggcgtcg
     1261 gcgatctggc gggtcgtcac catctcgccg ttctggagca gcagggaaag ggtggcgtcg
     1321 acgaccatct gccggcgctc gtcgggcggc agcgcgctgg cgcgcttgcc cggatggggc
     1381 gccaccactg tccccactgt tcccactgtt cccatggtcc ccgcaggtcc tcgcatgatc
     1441 tggccatcct agctgagtga gcattcactc acaaacgaga tcctcgtctt cggccacgac
     1501 ggggcttggg cgcggtgccc ggcccgccga tagaggacat atgtctcgtc tgtcgccctt
     1561 tgccggccgc gtagccgtgt gcggggtcgt gctcgccgcc ggcctggtac ccctcgcggc
     1621 ccgttcggcc tccgccgggg gcgcagtggt cgccatcgcg gcgcggcccc agggcgacgg
     1681 cttctggacc gccacctcgg acgggggcgt gctgcccagc ggcgccgctc ccaatctcgg
     1741 ttcgatggcc ggccgcgccc tggcggcgcc gatcgtgggg ctggcctcga ccgtcgacgg
     1801 caacggctac tggctcctcg ggcgggacgg agggatcttc tccttcggtg acgccgcctt
     1861 ctacggctcg accggcggca tgcgcctcaa ccagccggtg gtcggcatgg ccgcgacccc
     1921 gacggggcag ggctactggt tcgtggcggc ggacggcggg atcttctcct tcggtgacgc
     1981 cgccttctac ggctcgacgg gcgggctgcg cctcaaccag cccatcgccg ggatggccgc
     2041 cgccccgacc gggcgcggct actggctggt ggcgcgcgac ggcgggatct tcgcgttcgg
     2101 caacgccccg ttctacggct cgaccggatc gctccagctc ggcgcacccg tggtcgggat
     2161 gacggcgaca tcggacggca gcggctactg gttcgtggcg tcggacggcg gcatcttcgc
     2221 ctacggggac gcccccttcg ccggctccgc ggccggcgcg gccacctcgg ggcccatcgt
     2281 cgggatggcc gcccacccgg gaggcggcta ctggctggcc gaagcgagcg gcgccgattt
     2341 cccctacggc gtatcgccgg tcccgggcgg ggcgacacag acctcgtgcg gctacgtgcc
     2401 cccgacaccg gcctacaaca cgtcgtacgg cttcgagcgc gaccccaaca acggcgcgac
     2461 catgcggtgg gacccgcgga cgacgatcca ctacgtgatc gacccgaacg gggctccgac
     2521 cgacgtggag ggcgtggtcc agtcggccgt cgggaccgcc gcccaggtca gcgggctcca
     2581 gttcgcctac gacggcacct acgacagcgc ggccgacccc ggcccggccg gctcgcccct
     2641 gcgcttccgg tgggccaacc tgggcgccgg caccgagggt gggcgggcgg actacagctg
     2701 ggtgtacacc cccgggggga acaacgagct gatcgcggcc gtcgtcaacg tcaacaccgc
     2761 gtcgagcctg gcgcccgact acagcaacgg cgccagcgag ggcaccctcg tcctgcacga
     2821 gctcggtcac gccctcgggc tgggccacgc ctccgactcc agccagataa tgaacgcggt
     2881 cctctccccg agcggcccga cggggtatgg gcccggcgac cgcgacgggc tgtggcacca
     2941 gggcccggcc gccggctgcc tgaattagca ccgccgcggc cgcagcatcc ctcgtgaacc
     3001 gatgtgctag gcacgtcggt cgccgccatg cagtccgcag atcaccgccc ccgcccccgc
     3061 cccgccgctc tcgccggtct cgtcgcggcc gcgctggcgt tgggcgggtg cgggctgacg
     3121 agctgtggcg gcacgcggag cgcccatccg aggcacccgc ccaccaccgt gggctcgacg
     3181 ggacctccgg gcgtgccacc ggtcgtggac ccctccaacg tctacagcca ggcgggggcc
     3241 aacctgctga ccggtgccgc ccgctcggcc ctgccgcggg tctacgtgcc cgaccatggc
     3301 tcgaacaacg tgaccgtgat cgatcccgcc acccgcaggg tgctggcggt gctgcccgtc
     3361 ggcgccaacc cgcagcacgt ggtgccgtcc tacgacctgt ccacgctgtg ggtggccaac
     3421 aacgccgacg ggaccaacct gggcagcctg accccgatcg accccacgac gggccgagcc
     3481 gggccggcgg tggcggtcga cgatccctac aacctgtact tcacccccga cggcgcccac
     3541 gccatcgtgg tcgccgaggc ccggaagcga ctcgacttcc gggaccccag gaccatgcgc
     3601 ctccagtact ccatcgacgt gcggagctgc cccggcatca accacatgga cttctcgctg
     3661 gacggcagct acctgatcgc cacctgcgag gacgccggcc ggctggtcaa ggtcgactgg
     3721 acccggcgct cagtggtcgc caccctcgag ctcgggaccg cggccctgcc ccaggacatc
     3781 cggctggcgc ccgacggcag gctgttctac gtggccgaca tgatggggaa ccgcctcatc
     3841 ctcgtggacg gcgaccgcct cctgatcgcc ggctccgtgg acctggccgc gtcaggcggc
     3901 atcggcgccc acggcctcta ccccagccgc gacgccaggg tcctgtacgt ggccaaccgc
     3961 gggtcggcgg ggcgcgtcgg cgtggccccc cacggaccgg gcagcgtgtc ggtggtggac
     4021 ttcgccaccc gccaggtggt ggccaactgg ccggtgccgg gaggcggcag ccccgacatg
     4081 ggcggcgtga gcgccgacgg cacccagctg tggctcagcg gccggtacga caacgaggtc
     4141 tacgtcttcg acaccagggc cgggcgcctg gccgaccgca tcaccgtcgg accggctggg
     4201 cgcgagcccc acggcctgtg cgtctggccc cagcccggtc gctactcgct gggtcacacc
     4261 ggcaacctgc gataggcccg cgccggcgct cgccggggcg cgggcctgct tggatcaggc
     4321 gctggcctcg gcggccgcga tcagggcggg gacgatctcc tcccaggccc ggcccttgtc
     4381 caggtagagg tcggcgcccc ggtcgagcac gtcgatcagg gtcacgagat cggggaaggc
     4441 cgacacgacc acgatccggg cctccgggac cagccgccgg agctccggta gcgccgtcat
     4501 gccgtcaacg accggcatct gcaggtcgag caccacgatg tcgggccgcg tgcggcccac
     4561 cacggtgaag ccgtcgatcg cctggagcat ggtctcgacg aacaaccgga aatcctcgtc
     4621 gtcgtcgatc accacgaccc tggcggctcg atccgatccg tgccattcga gcgccatgcg
     4681 ccacctccga cctagaccca atgggtgctg gccatagtca tatcgaccgg agcgtcccgt
     4741 tcctgtagcc cgttgtgact agagcgaggg ccggaccggc cgcggcttgt acggtggcgg
     4801 ggccatgacg agtgagccca ccaccgtcct cgtggtcgac gacgagccac tggtcgtccg
     4861 gctggtcgag ctcaacctcg agatggacgg ctaccgcgtg gtggccgcct acgacggtcc
     4921 atccgggctt cgggccgccg gcgaggagca cccggatgtc atcgtgctcg acatgatgat
     4981 gccgggcatg gacggcctgg aggtggcccg acacctcaag tccgacccag gcacggccac
     5041 catcccgatc atcttcctgt cggctcgcag cagccccgcc gatgtggccg ccgggctggc
     5101 ggcgggcgcg gcggtctaca tggccaagcc gttcgacgcc gaggagctct cggcgaagat
     5161 cgccgagctg ctgaagggct agcgcgaagc accgtggctc gttgggcgag tgtggcgcgg
     5221 gtcgggctga ccgggctcgt gctggccgcc gcaggtggcg cgctgatcgc gtccgccgcg
     5281 gccgccgacc ggcgggtcga ccgggcccgg gccgcggccc gggcggacgc ggtccgcggc
     5341 gccggccagg ccgtggccac cgggctggcc tcggcgatcg cccaggcgca cggcgtcggc
     5401 gccgccatcc acctcggacc gacgccggcg cagagccagg acctggccgg cgcgctggcc
     5461 ggcgccgacc tgctcgacga cggcgcctac gtcgtggacg cccggggggc ggtggtggtc
     5521 acgaccgtcg cccgggccga cctcgctcac ctgctgcgaa cctggcccga gctggacgtg
     5581 gcattgcacg gcgccagcat ctcgggcctc tacgaggacc cgctcgagca caccgtcgac
     5641 ctgctcgtcg ccgcgcccgc cctgccgggc accgcgatcg tcggccggac ccgcgttccc
     5701 ggcggcaacc tggcccgggc cgtcagtcag atcccgatcc cctcgggaac ccagctcctg
     5761 gtggtggacg gccacaacga gctcgtcaac ggtctcgccg tctcgccgcc gggagtcgag
     5821 gtggcgacgg gcctgcgggc gatggtccag gccggccgca ccggcgccgc gggtctcggc
     5881 acggcccggg cggaaggtgg cgccgcattg gcggtggcgc acgccccggc agggaacggc
     5941 tgggtcgtcc tgatcaccca gccggcc
//
LOCUS       DASWGG010000376         2762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_209211, whole genome shotgun sequence.
ACCESSION   DASWGG010000376 DASWGG010000000
VERSION     DASWGG010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209211"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1801
                     /locus_tag="VF954_06905"
     CDS             149..1801
                     /locus_tag="VF954_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX9530847.1"
                     /translation="MEPRARLKDPREVERRRWYTLLVLCLSLMVIGLDNTVLNVALPT
                     LAHHHGGLGATASQLQWIVDAYTIVFAGLLLTAGSLGDRFGRYKALALGLGLFGAGSL
                     ASAFVGSANALIATRALMGVGGAFIMPSTLSILTNVFTNPLERGKAIGVWAGVSAAGI
                     GVGPLVGGFLLSHFWWGSVLLVNVPIVIAGLVLGFFLVPDSRDPSAPKLDPLGAVLSI
                     AGLASLLWAVIEGPTRGWGSGAILGAFAAGFVVLAAFLAWELTYSSPMLDIRVFNNPR
                     FSAASGAITLTFLALFGTLFLMTQYFQAVLGYSTLKAGAVFVPQAGVMLVFAPLSSVW
                     VQKLGNKVVVAGGLTMVAVMMLLFQTMNQSSHLWQVIGLTMLLGLGMAHVMAPATDSI
                     MGSLPRAKAGVGSAVNDTTRQVGGAVGVAVFGSILASRYGSRFTHALGRTVPGSVVSA
                     ARNSIGKTIDVASSPAGHPYAPALLAKGHASFVSGLHVAALVGAAVIALAVTGVLAWL
                     PARPRDEEVAAVLDVVPAGGGSWERAEPERVELERVELGWEA"
     gene            1798..2406
                     /locus_tag="VF954_06910"
     CDS             1798..2406
                     /locus_tag="VF954_06910"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9530848.1"
                     /translation="MSSLVDGDRAPSAPPASCARVGRPRSESAHQAILDAALEALVED
                     GYAGLTIEGIASRARVGKATIYRRWASMPELVVEALGSHVCPDLPVVDSGDVRADLQQ
                     VLQSALETMTGEDGQLMAAFAAEKSRHPELRDEFERVFVAERRARVRELIARGVASGQ
                     LPASTDVELLADAGFAILWQRLSLQRPLSPDLPGRIVRQFLG"
     gene            2415..>2762
                     /locus_tag="VF954_06915"
     CDS             2415..>2762
                     /locus_tag="VF954_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9530849.1"
                     /translation="MPPAKLHWAHLYDEVVTSGLCTGCAGCIVACPYDVLGYDDSNGV
                     YKPFQLDPAAAPANCIHGEKGCTLCTRACPRFRDWEPEIDTFLFGRPREIGEVEGVSK
                     DIVLARAVDQEVQD"
BASE COUNT          357 a          980 c          968 g          457 t
ORIGIN      
        1 ggctggggtg ccggcggtcc cacgtgacgg gcgctgggcc gggtgatccg gttgtcgccc
       61 gcctgctccg gaataattac gcaccgcgtg cgttccgata ctcggatcga cagacggatc
      121 aacggccaga tcgacagaga gggcggcaat ggaaccacgg gcacgtctca aggacccgcg
      181 agaagtcgag cggcggcgct ggtacaccct cctggtcctc tgcctcagcc tgatggtgat
      241 cggtctcgac aacaccgtcc tcaacgtcgc cctgcccacg ctggcccacc accacggcgg
      301 cctcggggcc accgccagcc agctgcagtg gatcgtcgac gcctacacca tcgtcttcgc
      361 cggcctcctg ctcaccgccg ggagcctcgg tgaccgcttc gggcgctaca aggccctggc
      421 gctcggcctg ggcctcttcg gggcgggctc gttggcatct gcgttcgtcg gctcggccaa
      481 cgccctcatc gcgacgcgcg ccttgatggg tgtcgggggc gcgttcatca tgccgtcgac
      541 attgtcgatc ctgaccaacg tcttcaccaa ccccctcgag cggggcaagg ccatcggcgt
      601 ctgggccggg gtctcagcgg ccggcatcgg cgtcggcccc ctggtcgggg gcttcctcct
      661 cagccacttc tggtggggct cggtgctgct cgtcaacgtg ccgatcgtga tcgccggcct
      721 cgtcctgggc ttcttcctcg tgcccgactc ccgtgatccc tcggcgccca agctcgaccc
      781 tctgggcgcg gtgctctcca tcgccggcct cgccagcctg ctgtgggccg tcatcgaggg
      841 cccgacacgg ggctggggca gcggcgccat cctcggggca tttgcggccg gcttcgtcgt
      901 gctggccgcc ttcctcgcgt gggagctcac ctactcgagc ccgatgctgg acatcagggt
      961 cttcaacaac ccccgcttct cggcggcgag cggcgccatc accctcacgt tcctggcgct
     1021 gttcgggacg ctcttcctga tgacgcagta cttccaggcc gtgctcggct attcgacgtt
     1081 gaaggccggg gcggtgttcg tcccgcaggc aggggtcatg ctcgtgttcg ccccgctttc
     1141 gagcgtgtgg gtgcagaagc tcggcaacaa ggtggtggtc gccggcggcc tcaccatggt
     1201 cgcggtgatg atgctgctgt tccagacgat gaaccagagc tcccacctgt ggcaggtcat
     1261 cggcctgacc atgctgctcg gcctcggcat ggcccacgtg atggcacccg cgaccgactc
     1321 gatcatgggg tccctgccgc gggccaaggc cggcgtcggc tcggcggtca acgacaccac
     1381 ccggcaggtc ggcggggcgg tgggcgtggc cgtcttcggc tcgatcctgg cgtcgcgcta
     1441 cgggagccgc ttcacccatg ccctgggcag gaccgtgccc ggctcggtcg tatccgcggc
     1501 gaggaactcc atcggcaaga cgatcgacgt ggcctccagc ccggcgggac atccctacgc
     1561 gccggcgctg ctcgccaaag ggcatgccag cttcgtgtcc ggcctgcacg tggcggcact
     1621 cgtgggcgcg gcggtcatcg ccctcgccgt gaccggcgtt ctggcctggt tgcccgcccg
     1681 gccgcgggac gaggaggtcg ccgcggtcct cgacgtcgtg cccgccggcg gcggctcgtg
     1741 ggagcgggcc gagccggagc gggtcgagct ggagcgggtc gagctgggat gggaggcgtg
     1801 agcagcctgg tcgacggcga ccgcgccccg agcgcaccgc cggcctcgtg cgcccgggtg
     1861 ggccggcccc ggtccgaatc ggcccaccag gccatcctcg acgccgccct cgaggccctc
     1921 gtggaggacg gctatgccgg cctcaccatc gaggggatcg cctcccgggc cagggtcggg
     1981 aaggccacga tctaccgccg atgggcctcg atgcccgagc tggtggtgga ggccctgggt
     2041 agccacgtgt gcccggacct gcccgtcgtc gactcgggcg acgtgcgggc ggatctccag
     2101 caggtgctcc agagcgcgct cgagaccatg acgggcgagg acgggcagct catggcggcg
     2161 ttcgcggcgg agaagtcccg acacccggag ctgcgcgacg agttcgagcg ggtcttcgta
     2221 gccgagcgcc gggcccgggt ccgcgagctc atcgcccgcg gagtggcatc gggtcagctt
     2281 cccgcgtcca cggacgtcga gctactggcc gacgcgggtt tcgcaatcct gtggcagcgc
     2341 ctgagcctcc agcgcccgtt gtcgccggac ctgccggggc ggatcgtccg ccagttcctg
     2401 gggtagcttc gcgcatgcct cccgccaagc tgcactgggc ccacctctac gacgaggtgg
     2461 tgacgtcggg gctctgcacg ggatgcgccg gatgcatcgt ggcctgcccc tacgacgtgt
     2521 tgggctacga cgacagcaac ggcgtgtaca agcccttcca gctggacccg gcggccgctc
     2581 ccgccaactg catccacggc gagaaggggt gcaccctgtg cacccgggcc tgcccccgat
     2641 ttcgcgattg ggagccggag atcgacacct tcctgttcgg ccggcccagg gagatcggcg
     2701 aggtcgaggg ggtctccaag gacatcgtgc tggcccgtgc ggtcgatcaa gaggtccaag
     2761 at
//
LOCUS       DASWGG010000377         3453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_541271, whole genome shotgun sequence.
ACCESSION   DASWGG010000377 DASWGG010000000
VERSION     DASWGG010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541271"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VF954_06920"
     CDS             <1..385
                     /locus_tag="VF954_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX9530850.1"
                     /translation="VMYAGKVVEKAPTHVLFSEMRMPYTEALLKSIPRIEYPSHTRLV
                     AITGRPPDLVLPPPGCHFAPRCPYAQERCHVEEPPLLPGDTPGHEFACFYPVGTPEGH
                     AALEDNLSAGLAQTVAAVEGTEVGA"
     gene            386..1426
                     /locus_tag="VF954_06925"
     CDS             386..1426
                     /locus_tag="VF954_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX9530851.1"
                     /translation="MAGTGSAHLRPQQALLSVEHLVVEFPAGRRRRVHAVSDVSFDVL
                     AGETMGLVGESGCGKSTTGRAVVQLPRPTGGSVLFDGQDMTRLKGEELRRTRIKMQMI
                     FQDPISSLNPRRRLKDIVAEPLRIWKVGDELSREAKVREVLDTVGLDFDTQAHRRPHE
                     FSGGQCQRISIARALVLDPKVIICDEPVSALDVSVQAQILNLLEDMKARYGLTLIFIA
                     HDLAVVKNISDRVAVMYLGKLCEVAAPDELYRRPSHPYTAALLASIPVPNPEVRPGMT
                     GASLAGELPSPINPPSGCRFRTRCPRAQVRCAEEEPVMREIGESHYLACHFPLGAPEA
                     PAQGIPATAASG"
     gene            complement(1434..2009)
                     /locus_tag="VF954_06930"
     CDS             complement(1434..2009)
                     /locus_tag="VF954_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530852.1"
                     /translation="MSPCPACGRPLSPASAPGGRRYLCAACHLVVVGLPVLRAAIGRP
                     AEAAVWLASETVAAGDHPCPFCAQGMRPVQAPPGPAGRAQAQVCRSCEVVAVAADQWP
                     LLPQQPADPAVAAPTAATDLRCPTCGAPRQATPDNCCRYCGGKLAMEPTVVFVPTAAS
                     GDRFGDAPGKIEAAASLLSALTRVVGWAAGS"
     gene            2116..2775
                     /locus_tag="VF954_06935"
     CDS             2116..2775
                     /locus_tag="VF954_06935"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEX9530853.1"
                     /translation="MDVTPDLVPEEVRVLGALVEKQLTTPQHYPLTLNALAAACSQSS
                     NRWPVVSYGEATVSAALGCLRERGLIRVVHSTSNRAAKYRHVLDEALGLDRPAVATLG
                     VLLLRGPQTVGELRGRTERMHPFESLVEVEEVLEALAGRPDPLVVRLPRQPGQKDARY
                     VHLMSGMPDGPAAAEPVGAEGQAQAPGPDVPALEGRVAALEDEVAELRAGLEELRELF
                     R"
     gene            complement(2845..>3453)
                     /locus_tag="VF954_06940"
     CDS             complement(2845..>3453)
                     /locus_tag="VF954_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530854.1"
                     /translation="DSAGYPAFNLTAAKAAVQAYSAAHGGKPLTVELDTTTDSKGLQV
                     MQLVQSMWQAAGMQVTLKQFEQSTYIINALQGKFQAANFRQFGEPDPDVNYVSWSTAS
                     AMPLGAFSPNIDRYKDPQIDADLEKGRTSADPAVRKQAYQDIARELNKGLPYIYLAEY
                     SFSTFYKPNIHGVVDWTLPDGAKGVNYTSDGIQRFANWWVAP"
BASE COUNT          487 a         1246 c         1226 g          494 t
ORIGIN      
        1 ggtgatgtac gccggcaagg tggtggagaa ggccccgacc cacgtcctgt tctccgagat
       61 gcgtatgccg tacaccgagg ccctgctgaa gtcgatcccg aggatcgagt acccgagcca
      121 cacccggctg gtggcgatca ccggtcgccc gcccgacctg gtcctgccac cgccaggctg
      181 ccacttcgcc ccccgctgcc cgtacgccca ggagcgatgc cacgtggagg agcccccgct
      241 gttgcctggc gacacgcccg gccacgagtt cgcctgcttc tatccggtgg gcacccccga
      301 aggccacgcc gccctcgagg acaacctgtc ggccgggctg gcccagaccg tggccgccgt
      361 cgagggcacc gaggtcgggg cctaggtggc cggcaccggc tcggcccatc tccggcccca
      421 acaggccctg ctcagcgtcg aacacctggt ggtcgagttc cccgcagggc gtcgccggcg
      481 ggtccacgcc gtctccgacg tgagcttcga cgtcctcgcg ggcgagacga tgggcctggt
      541 gggcgagtcg ggctgcggca agtcgaccac cggtcgcgcg gtcgtgcagc tgccccggcc
      601 gacgggcggc tcggtcctct tcgacggcca ggacatgacc cggctcaagg gcgaggagct
      661 gcgccggacc cggatcaaga tgcagatgat cttccaggac cccatctcgt cgctcaaccc
      721 ccggcggcgc ctcaaggaca tcgtcgccga gcccctccgc atctggaagg tcggcgacga
      781 gctgtcccgg gaggccaagg tccgcgaggt gctggacacg gtcggcctcg acttcgacac
      841 ccaggcccac cgccggcccc acgagttctc cggcggccag tgccagcgga tcagcatcgc
      901 ccgggccctc gtgctcgacc ccaaggtcat catctgcgac gagcccgtct ccgcgctgga
      961 cgtgtccgtc caggcccaga tcctcaacct gctcgaggac atgaaggccc gttacggcct
     1021 gacgctgatc ttcatcgccc acgacctggc cgtggtcaag aacatcagcg accgggtggc
     1081 ggtcatgtac ctcggcaagc tgtgcgaggt ggccgcgccc gacgagctgt accgccggcc
     1141 gtcccatccc tacacggccg cccttctcgc ctccatcccg gtccccaacc cggaggtccg
     1201 cccgggcatg accggcgcca gcctggctgg cgagctgccg tcgccgatca accctccctc
     1261 ggggtgccgt ttccgcaccc gttgcccccg ggcccaggtc cggtgcgccg aggaggagcc
     1321 cgttatgcga gagatcggcg agtcgcacta cctggcctgc cacttcccgt tgggtgcgcc
     1381 cgaggcgccc gcccagggca tcccggcgac ggccgcgtcg ggctgagaag cagctaggag
     1441 ccggcggccc accccacgac ccgggtgagc gccgacagga gggacgcggc ggcctcgatc
     1501 ttccccgggg cgtcaccgaa gcggtctccg gatgcagcgg tcggcacgaa gaccacggtg
     1561 ggctccatgg ccagcttccc accgcagtag cggcagcagt tgtccggggt ggcctggcgg
     1621 ggcgcgccgc aggtggggca gcggaggtcg gtcgcggccg tgggcgcggc caccgcggga
     1681 tcggccggct gctggggcag aaggggccac tggtcagccg ccaccgcgac cacctcgcag
     1741 ctccggcaca cctgggcctg ggcgcggccc gccggccccg gcggcgcctg gaccggtcgc
     1801 atcccctggg cgcagaacgg gcaggggtga tcgcccgcgg ccaccgtctc gctggccagc
     1861 cacacggcgg cctctgccgg ccggccgatg gcggcacgca gcacggggag gccgaccacg
     1921 accaggtggc aggcggcgca caggtagcgc cggccgcccg gcgcgctggc cgggctgagg
     1981 gggcggccgc acgcggggca cggagacatg gcggtgtcca tcaaggcgcg gtgtccatcg
     2041 aggcgcggtc catcaagcag tagtcggcac gaacaccgcc cagcattatc ctgacccgcc
     2101 attatcgtga cgcccgtgga cgtcacgccc gacctcgtgc ccgaggaagt ccgggtgctg
     2161 ggcgccctgg tcgaaaagca gctgaccacg ccgcagcact acccgcttac tctcaacgcc
     2221 ctggcggccg cgtgcagcca gtcctccaac cgatggccag tcgtgagcta cggggaggcg
     2281 accgtgtcgg ccgcgctggg ctgcctgcgg gagcgagggc tcatccgcgt ggtgcacagc
     2341 acctccaacc gggcggccaa gtaccgccac gtgctcgacg aggcgctcgg tctggaccgg
     2401 ccggccgtcg ccaccctcgg cgtcctcctg ctccgcgggc cgcagacggt tggcgagctc
     2461 cggggccgga cggaacgcat gcaccccttc gagagcctgg tcgaggtgga agaggtcctc
     2521 gaggctctgg ccggccggcc cgatcccctc gtggtccgcc taccccgcca gcccggccag
     2581 aaggacgccc gctacgtcca cctgatgagc gggatgcccg atggccccgc ggccgccgag
     2641 cccgtcggcg ccgaaggcca ggcgcaggcg ccgggcccgg acgttcccgc gctcgagggc
     2701 cgggtggccg ccctggagga tgaggtcgcc gagctgcggg ccgggctgga ggagcttcgc
     2761 gagctgttcc ggtaggagcg agaagggcgg cccccttggt tcaagagggc cgccccttcg
     2821 cctgatgagc gatggcgctg gtcgttaggg ggcaacccac cagttggcga accgctggat
     2881 cccgtcggag gtgtagttga cgcccttggc gccgtcgggg agcgtccagt ccaccacgcc
     2941 gtggatgttg ggcttgtaga acgtcgagaa ggagtactcg gcgagataga tgtacggcag
     3001 gcccttgttg agctccctgg cgatgtcctg gtacgcctgc ttgcggacgg ccgggtccgc
     3061 tgatgtccgg cccttctcca ggtcggcgtc gatctggggg tccttgtacc ggtcgatgtt
     3121 cgggctgaac gcgcccaacg gcatggcgct ggccgtcgac cacgagacgt agttgacatc
     3181 ggggtccggc tcgccgaact ggcggaagtt ggcggcctgg aacttcccct ggagggcatt
     3241 gatgatgtag gtgctctgct cgaactgctt cagcgtgacc tgcatgcccg ccgcctgcca
     3301 catgctctgg accagctgca tgacctgaag gcccttggag tcggtggtcg tgtccagctc
     3361 gacggtgagc ggcttgccgc cgtgagccgc ggagtaggcc tggacggccg ccttggccgc
     3421 ggtgaggttg aaggcggggt acccggccga gtc
//
LOCUS       DASWGG010000378         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2148372, whole genome shotgun sequence.
ACCESSION   DASWGG010000378 DASWGG010000000
VERSION     DASWGG010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2148372"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1896)
                     /locus_tag="VF954_06945"
     CDS             complement(235..1896)
                     /locus_tag="VF954_06945"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX9530855.1"
                     /translation="MADLVRIAFLGGLGEIGRNCAAFEQDGRIVLLDCGLMFPEAEMP
                     GIDLVLPDFTWLREHADQVEACILTHGHEDHAGGLAFLLRELSFPIYGSPLTLALARG
                     RLEEAGVLDRTTLVPVHDGERRDIGPFDVEFIPVTHSVPHGFATAFFTPQGVILHSGD
                     FKLDLTPVDGRLTDLARIGAINKQHGIRLLLADSTNAEEPGHTPSETTVGVVLHDLFE
                     RHRDKRMVVTCFASHLHRIQQVADAAMASGRKLATLGRSMAKNVALGRDLGLLHLPAD
                     ALIDIERIGDLKPSEVCIISTGSQGEPMSALSLMAAGENKWLKVGEGDVVVISAHAIP
                     GNESAVSKVIDGLHRRGCEVIHSGLADVHVSGHAMQGELAMLQSVAQPDYFVPVHGEY
                     RHLIHHARLAVRMGVAPPNVLLCEDGDVIALRPDGVDFDGEVPAGYLYVDGIVGDVGH
                     GVLRDRRVLAEEGVVVVVVTVDARTCEVVSGPEIITRGWVYAPEAEDLLGEARAAVLA
                     GLEAAADEGVADLETLRRHVRAALGRFVNERTRRRPMIVPVIMEV"
     gene            complement(1889..>2669)
                     /gene="dapA"
                     /locus_tag="VF954_06950"
     CDS             complement(1889..>2669)
                     /gene="dapA"
                     /locus_tag="VF954_06950"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX9530856.1"
                     /translation="VAGTTGEAPVLSDDEKLTLWRAVVEAVDVPVIAGSGGNDTHHSV
                     ALTRAAGHLGVAGILAVTPYYNRPSQAGLAAHFRAVAEATTLPVMLYDIPVRAGRKIA
                     HDTLIALARAVPNIVAVKDAAGNPAASARVVAESPDGFELYSGDDSMTLPLLAIGASG
                     SVGVATHWTGALHQEMMAAFAKGDVDRARAVNARMLPSFAFESSDEAPNPVPTKAMLS
                     VLGLPGGDCRLPMGPPPAGLDERARAVLAALGPDAPPPARG"
BASE COUNT          384 a          944 c          983 g          358 t
ORIGIN      
        1 ccgacgagcg ggatcgcggc gcccgccggc gggcgggcgc cttgcgccgg gctggctgcc
       61 tgcgcgccgc ttgccggagc gccggttgct tccgccgggc cgatgccggc cgccgggtcc
      121 tgccccgtgg gtccccgcca cgggcccggg gggcggccga tcgggtgcga gtgctcgtca
      181 cagtgaccac aaccgtaaca ccagccacac ggccatccgg ccaccgccgt cggcctagac
      241 ctccatgatg accggcacga tcatcggacg gcggcgcgtg cgctcgttga cgaagcgccc
      301 cagcgcggcg cgcacgtggc ggcgcagcgt ctccaggtcg gcgacgcctt cgtcggcggc
      361 cgcctcgagg ccggcgagga ccgcggcccg ggcctccccc agcaggtcct cggcctcagg
      421 cgcgtacacc cagccccggg tgatgatctc cgggccactc accacctcgc aggtccgggc
      481 gtccacggtg acgacgacca ccacgacgcc ctcctcggcc agcacccggc ggtcgcggag
      541 cacgccgtgg cccacatcac cgacgattcc gtcgacgtac aggtagccgg ccgggacctc
      601 gccgtcgaag tcgacgccgt cgggccgcag cgcgatcacg tcgccgtcct cgcacagcag
      661 cacgttgggc ggggcgacac ccatccgaac cgccagccgg gcgtgatgga tgagatgccg
      721 gtactcgcca tggaccggta cgaagtagtc gggctgggcc acggactgga gcatggccag
      781 ctcgccctgc atggcgtggc cggacacgtg cacgtccgcc aggccggaat ggatgacctc
      841 gcagccgcgg cgatgcaggc cgtcgatcac cttgctcacc gccgactcgt tgccggggat
      901 ggcgtgggcg ctgatgacga ccacgtcgcc ctcgcccacc ttcagccatt tgttctcacc
      961 ggcggccatc agggacaggg cggacatggg ctcgccctgc gagccggtgg agatgatgca
     1021 cacctcgctg ggcttcaggt cccctatgcg ctcgatgtcg atcagggcgt cggccgggag
     1081 gtggagcagg cctaggtcgc ggcccagggc gacgttcttg gccatcgacc ggccgagcgt
     1141 ggccagcttg cggcccgagg ccatggccgc gtccgcgacc tgctggatgc ggtggaggtg
     1201 gctggcgaag caggtgacca ccatccgctt gtcccggtgg cgctcgaaca ggtcgtggag
     1261 caccacgccc acggtggtct ccgacggggt gtgaccgggc tcctcggcgt tggtggagtc
     1321 cgccaaaagg aggcggatgc catgttgctt gttgatggcc ccgatacggg ccaggtcggt
     1381 caggcgaccg tccacgggcg tgagatcgag cttgaagtcg cccgagtgga ggatgacgcc
     1441 ctgcggggtg aagaaggcgg tcgcgaagcc gtgggggaca gagtgcgtca ccgggatgaa
     1501 ctcgacgtcg aagggcccga tgtcgcgccg ctcgccgtcg tgcaccggga ccagcgtggt
     1561 ccggtcgagg acgccggcct cctccagccg gccccgggcc aacgcgaggg tcaggggcga
     1621 tccgtagatc gggaatgaca gctcgcgcag caggaacgcc agcccaccgg cgtggtcctc
     1681 gtgcccgtgg gtcaggatgc acgcctccac ctgatcggcg tgctcgcgca accaggtgaa
     1741 gtcgggcagg acgaggtcga tccccggcat ctcggcctcg gggaacatca gcccgcagtc
     1801 gagcagcacg atgcgcccgt cctgctcgaa cgccgcgcag ttccgcccga tctccccgag
     1861 cccgcccagg aaggcgatgc ggacgagatc agccacgcgc cgggggcgga gcgtcagggc
     1921 cgagggcggc cagcacggcc cgggcgcgct catccagccc tgccggcggc ggccccatcg
     1981 gcaggcggca gtcgccgccc ggcaacccga gcacggagag catggccttg gtggggaccg
     2041 ggttgggcgc ctcgtccgag ctctcgaagg cgaacgacgg cagcatcctg gcgttgacgg
     2101 cgcgcgcccg gtcgacgtcg ccttttgcga aggcggccat catctcctgg tgcagggccc
     2161 cggtccaatg cgtcgccact ccgacggagc ccgacgcgcc gatggcgagc agcggcaggg
     2221 tcatcgagtc gtcgccgctg tagagctcga agccgtccgg cgactcggcc acgacgcggg
     2281 ccgacgccgc ggggttcccg gccgcgtcct tgacggccac gatgttgggc acggcgcggg
     2341 ccagggcgat caaggtgtcg tgggcgatct tgcgcccggc gcgaacgggg atgtcgtaca
     2401 gcatcacggg cagggtggtg gcctcggcca ccgcccggaa atgggccgcc aggccggctt
     2461 gggagggccg gttgtagtac ggcgtcaccg ccaggatgcc ggccactccg agatggccgg
     2521 cggccctggt caaggcgacc gaatggtggg tgtcgttgcc tcccgagccg gcgatcaccg
     2581 ggacgtcgac cgcctcgacc acggctcgcc agagggtcag cttctcgtcg tcggacagga
     2641 cgggcgcctc gccggtcgtg cccgcgacg
//
LOCUS       DASWGG010000379         5626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_543186, whole genome shotgun sequence.
ACCESSION   DASWGG010000379 DASWGG010000000
VERSION     DASWGG010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543186"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VF954_06955"
     CDS             complement(<1..829)
                     /locus_tag="VF954_06955"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX9530857.1"
                     /translation="MPEWNPSAWRELPATHQPAWPDQAALDRALKRLNALPPLVFAGE
                     ARRLTGALAEVADGRAFLLQAGDCAESFSEFSANAIRDKLRVILQMAVVLTYGSGVHV
                     VKVGRIAGQFAKPRSSPTEWVGGVELPSFLGHIVNDDAPTAAARRPDPERLLGAYHQS
                     AATLNLLRAFTTGGFADLSQVHMWNQQFVATSAEGRRYEAIAGEIDRALRFMRACGID
                     LDTDANLHQVEFWTSHEALLLSYEEALTREDTLTGDWYDCSAHMLWIGERTRQVDGAH
                     "
     gene            919..2016
                     /locus_tag="VF954_06960"
     CDS             919..2016
                     /locus_tag="VF954_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530858.1"
                     /translation="MATTPDRKAAVVPIVVVIASLALIAAGVVVATSVHHASRAGASE
                     ATRDQREREATTSWDQQASDAFHPLATAVADLTARTGTWLAGTLPTDAYQAALASDVP
                     VILNGRNLVGGLPAFPYDRRANDMYWDASLLYTELARVDVTLIDLQAGPLRDQEGLAA
                     RRLGELADRVFHRGRALVLPFRHDAPAAGAAADPPDDVPSWTVEGLTAGPPLDTVPPA
                     ASGTSQASRPQEPKPQWKDDVDRAGIPTLPALTSAVDGTESASAGAGLATQQRNLADA
                     YLSAAAALRDRPDPAGDREEGARLRLSLLVYAEAARQAEAGTSASDPTAAERLDAAGR
                     RLLLIGDALWPPDLGGRNSGEPGDILHDQGA"
     gene            2074..2436
                     /locus_tag="VF954_06965"
     CDS             2074..2436
                     /locus_tag="VF954_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530859.1"
                     /translation="MARVPPDDPEDWSNDEWLDYLAETDAAAAPAPGRPVSAAGAFAR
                     SGAGGAIGAAMKGLHRAIYGGQEDEIAIVADASGDPPGPEGLDVHLDPDDPGRSTVVV
                     KRPVDAARAPQLDEPPDP"
     gene            2448..3068
                     /gene="nadD"
                     /locus_tag="VF954_06970"
     CDS             2448..3068
                     /gene="nadD"
                     /locus_tag="VF954_06970"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX9530860.1"
                     /translation="MRLGIFGGTFDPIHNGHLVAAVNARHDVGLDRVLIMPAHVPWQK
                     VGHRAVTPAADRLAMVAAAVTDVDGLEASDLEVRRGGWSYTADTVEALAVAQPAAGLF
                     VILGADAVAGLGTWERAETIRALASLVVVNRPGAGAGAVAVPPGWRGVTIEIPALDLS
                     STDLRARAAAGRPLDYLVPPGAIRYLRERGLYAGGHPDEPHPRPAP"
     gene            3040..4347
                     /locus_tag="VF954_06975"
     CDS             3040..4347
                     /locus_tag="VF954_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX9530861.1"
                     /translation="MSPTPGLPLEPLELNEDPRPSGALFDQDEGSALQAPQAPPGVEL
                     EPIPMPAVTTSRRARRRRRRQRDQRLLAGTITASLVVVGALVVLAAGLFAGRQVVRQV
                     TGHPSAATAPPPPPPPVLFVEQDPARAAVALAVLAPASGGKGGSILLIDPGTMAEVPA
                     LGLQPLSQALALGGSDRLRTSVENLLGVQIGEIDVLDPAGVVGLVGASSTLRVDVPEA
                     VESVAPGGLVDTVFAAGPQDVAAGRVPALLATPGASGDLARVARQEAFFHAWLAQVRE
                     DAAGLGTGPPGLRGAVAALAAAQSSVRVLPVSSLGKADDGTQLYQVDLGQAGTVLGEV
                     FPSSHPDPNRPSVQVLNGTGALGVAERVTEKLLPAGVQVKLTDNADRLNYPQTQIVYY
                     DQRRATIAQRVRNALGVGTLVLSRRPIDVVDVTVIVGKDFPNG"
     gene            4347..4733
                     /gene="rsfS"
                     /locus_tag="VF954_06980"
     CDS             4347..4733
                     /gene="rsfS"
                     /locus_tag="VF954_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX9530862.1"
                     /translation="MTRQLEESKAVAVAAARAAAAKKGQDTVILDVGPVLAIADLFVI
                     TSAANARQARTVAEEVEARVATALARRPLRIEGLDDARWVLMDYGDVIVHILLEDARA
                     YYDLERLWADAAVVAWDDVARAVAGE"
     gene            complement(4793..>5626)
                     /locus_tag="VF954_06985"
     CDS             complement(4793..>5626)
                     /locus_tag="VF954_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9530863.1"
                     /translation="TRSKAKTVFVLGGGGNLGAIQVGMLRAALERGIRPDVVVGCSVG
                     AINAAALAADPTPEGARQLQEAWLGVHNDMLCPSSRLSSVRLLTRKARSLTANDGLRN
                     LLRQWLPYHLLEEAAIPVEVVATDLRTGKEHWFATGSVDEAVLASAALPAVFPPVVID
                     GTPYIDGGVVNNVPISRAVELGATRVVVMHVGNFERPRPEPRRPIDVLLQSFSIARNH
                     RFLAEVGSTPEGVEMIVLPGIDPGRLRYNDFSRSRMLMERAYATSSAFLDASHLVVAG
                     A"
BASE COUNT          736 a         2069 c         2089 g          732 t
ORIGIN      
        1 catgggctcc gtcgacctgg cgggtccgct caccgatcca gagcatgtga gccgagcagt
       61 cgtaccagtc gccggtgagg gtgtcctcgc gcgtgagggc ctcctcgtag ctgagcagca
      121 gcgcctcgtg gctggtccag aactccacct ggtggaggtt ggcatcggtg tccaggtcga
      181 tgccgcatgc ccgcatgaag cgcagggccc ggtcgatctc gcccgcgatg gcctcgtacc
      241 gacggccctc ggcgctggtg gccacgaact gctggttcca catgtggacc tgggagaggt
      301 cggcgaagcc tccggtggtg aaggcccgga ggaggttcaa ggtggcggcg gactggtggt
      361 aggcaccgag gagccgttcc gggtcggggc gccgggcggc ggccgtcggg gcgtcgtcgt
      421 tgacgatgtg gccgaggaag gagggcagct cgacgccgcc cacccactcc gtcggggagg
      481 agcggggctt ggcgaactgg ccggctatcc gcccgacctt caccacgtgg acgccggatc
      541 cgtaggtcag gacgacggcc atctggagga tgaccctcag cttgtcgcgt atggcgttgg
      601 cggagaactc gctgaaggac tcggcgcagt cgccggcctg gaggagaaag gcccgcccgt
      661 cggccacctc ggccagcgcc ccggtcagcc ggcgcgcctc ccccgcgaac accagcggcg
      721 ggagggcgtt gagccgcttc agggcgcggt cgagggcggc ctggtcaggc caggccggtt
      781 ggtgggtggc gggcagctcc cgccaggcgc tcgggttcca ctcgggcacg cctacagggt
      841 ggacgacaac ggcgggatag gcaaagcact tgcccgggac ggcgcgggga ccgggcgccg
      901 ctagcctgct gggcgaccat ggcgacgact cccgatcgca aggcggcagt ggtgccgata
      961 gtggtcgtga tcgcgtctct cgcgctgatc gcggctgggg tggtggtggc gacctctgtc
     1021 catcacgcca gccgggccgg ggcgtccgag gccacgcggg accagcgcga gcgggaagcg
     1081 accacgtcgt gggaccagca ggcgtccgac gctttccacc ccttggccac cgcggttgcc
     1141 gacctcacgg ccaggaccgg cacatggctg gccggcaccc tcccgaccga tgcctaccag
     1201 gcggccctgg cgtccgacgt gcccgtcatc ctcaacggtc gcaacctggt ggggggcctg
     1261 cccgccttcc cctacgaccg gcgggccaac gatatgtact gggacgccag cctgctgtac
     1321 accgagctgg cccgggtcga cgtcacgctc atcgatctcc aggccgggcc gttgcgtgac
     1381 caggagggcc tggccgcccg gcgcctgggg gagctggccg accgggtctt ccaccggggc
     1441 cgcgccctgg tgctgccgtt ccgccacgat gcccccgccg ccggcgcggc agccgatccc
     1501 cccgacgacg tgccgagctg gacggttgag gggctcaccg ccggtccgcc actcgacacc
     1561 gtgccgcccg ccgcctccgg gacgagccag gcgtcccggc cccaggagcc gaagccgcaa
     1621 tggaaggacg atgtggaccg ggcggggatc cccacgttgc cggcgctcac gagcgcggtc
     1681 gacggcaccg aatcggccag cgccggcgcc gggctcgcca cccagcagcg caacctggcc
     1741 gacgcctatc tgtcggcggc cgcggccctg cgtgaccggc cggacccggc cggcgaccgc
     1801 gaggagggcg cccgcctgcg cctgagcctg ctcgtctacg ccgaggccgc ccggcaggcc
     1861 gaggccggca cgtcggcgtc cgaccccacg gcggccgagc gcctcgacgc cgccggccgg
     1921 cgcctcctcc tgatcggcga cgctctgtgg ccccccgatc tgggcggccg caacagcggc
     1981 gagcccggcg acatcctcca cgaccagggc gcctgagcgc ccggggcccc acctggcggc
     2041 cccgacttcc acctgccggg tctaccgtgg tttgtggccc gcgtcccgcc cgacgacccc
     2101 gaagactggt cgaacgacga atggctcgac tacctggcgg agacggatgc cgcggcggcc
     2161 ccggccccgg gccggcccgt gtcggcggcg ggagcgttcg cccgctcggg tgcgggaggg
     2221 gcgatcgggg ccgccatgaa gggcctgcac cgggcgatct acggcggcca ggaggacgag
     2281 atcgccatcg tcgccgacgc cagcggcgac cccccgggcc cggaaggcct ggatgtgcac
     2341 ctcgaccccg acgacccggg caggtcgaca gtcgtcgtga agcgcccggt ggacgccgcc
     2401 cgggccccgc agctggacga gccgcccgat ccctaggatg cgccggcatg cggctcggga
     2461 tcttcggggg cacgttcgac cccatccaca acgggcacct ggtcgcggcg gtcaacgccc
     2521 gccacgacgt gggtctcgac cgggtcctga tcatgccggc ccacgtgccc tggcagaagg
     2581 tgggccatcg ggcggtgacc ccggcggcgg accgcctcgc catggtcgcg gcggcggtca
     2641 ccgacgtgga cgggctcgag gcgagcgacc tcgaagtccg ccggggcggg tggtcctaca
     2701 ccgccgacac ggtcgaggcc ctggccgtgg cccagccggc tgccggtctg ttcgtgatcc
     2761 tcggggcgga cgccgtcgcc ggcctgggca cgtgggagcg cgccgagacg atccgcgccc
     2821 tcgccagcct ggtggtcgtc aaccggcccg gcgccggcgc cggcgcggtc gcggtgccgc
     2881 ccggctggcg gggcgtcacc atcgagatcc cggccctcga cctgtccagc acggacctgc
     2941 gcgcccgagc cgccgccggt cgtcccctcg actatctggt gccccccggg gcgatccgtt
     3001 acctcaggga acggggtctg tacgctggcg ggcaccctga tgagccccac ccccggcctg
     3061 ccccttgagc cccttgagct gaacgaagac ccgcggccct cgggcgccct gttcgaccag
     3121 gacgagggga gcgccctgca ggccccgcag gccccgccgg gggtcgagct ggagcccatc
     3181 ccgatgccgg cggtcaccac gtcccggcgg gcgcgccgcc gccggcggcg ccaacgggac
     3241 cagcggctgc tggccggcac catcaccgcc tctctcgtgg tggtcggcgc cctggtggtg
     3301 ctcgcagccg gactgttcgc cggccgccag gtcgtgcgcc aggtcacggg tcacccgtcg
     3361 gcagccaccg cgccgccccc gcccccgccc ccggtcctgt tcgtcgagca ggacccggcc
     3421 cgcgccgccg tcgccctcgc cgtgctcgcc ccggcttcgg gcggcaaggg aggctcgatc
     3481 ctgctcatcg accctggcac catggccgag gtgcccgccc tcgggctgca accgttgagc
     3541 caggccctgg ccctcggcgg ctccgaccgg ttgcggacca gcgtggagaa cctgctcggc
     3601 gtccagatcg gcgagatcga cgtgctcgac cctgccggcg tcgtcgggct ggtcggcgcc
     3661 agctcgacgt tgcgcgtgga cgtgcccgag gccgtggaga gcgtcgcgcc gggtggcctg
     3721 gtcgataccg tgttcgcggc cgggccccag gacgtggcgg ccggccgggt gcccgccctg
     3781 ctcgccacgc ccggcgcgtc gggggacctc gctcgggtgg cccgccagga ggccttcttc
     3841 cacgcctggc tggcgcaggt ccgggaggac gccgccggcc tcgggaccgg gcccccaggg
     3901 ctgcggggcg cggtggcggc gctcgccgcc gcccagagct cggtgcgggt ccttccggtc
     3961 tcgagcctcg ggaaggcgga cgacggcacg cagctgtacc aggtcgacct cggccaggcc
     4021 ggcacggttc tgggcgaggt gttcccgagc tcccaccccg accccaaccg cccctcggtc
     4081 caggtcctca acggcacggg cgcgctgggc gtggccgaac gggtgacgga gaagctgctg
     4141 ccggccggcg tgcaggtcaa gctcaccgac aacgccgacc ggctcaacta cccgcagacc
     4201 cagatcgtgt attacgacca gaggcgggcg accatcgccc agcgggtccg caacgcgttg
     4261 ggagtgggca cactggtgct gagccgccgg cccatcgacg tggtcgacgt gacggtgatc
     4321 gtaggaaagg acttcccgaa cgggtgatga cgcgacagtt ggaggagagc aaggccgtgg
     4381 ctgtcgccgc ggcgcgggcc gcggcggcga agaagggcca ggacaccgtc atcctggatg
     4441 tggggccggt gctcgccatc gccgacctct tcgtgatcac cagcgccgcc aacgcccgcc
     4501 aggcccggac cgtcgctgag gaggtcgagg cccgggtggc cacggcgtta gcgcgccggc
     4561 ccctgcggat cgagggcctc gacgacgccc gctgggtcct catggactac ggcgatgtga
     4621 tcgtgcacat cctcctcgag gatgcgcggg cgtactacga cctggagcgg ctgtgggccg
     4681 acgccgccgt ggtggcgtgg gacgacgtgg cccgggcggt ggccggcgag tagccctgcc
     4741 tccgccgtct catcagacgc cggctcagtc agacgccggc tcagatcccg gctcaggcgc
     4801 cggcgaccac gaggtggctg gcgtcgagga aggccgagct ggtggcgtag gcccgctcca
     4861 tcagcatccg cgatcggctg aagtcgttgt agcgcaaccg gccggggtcg atgcccggca
     4921 gcacgatcat ctccaccccc tcgggggtgg agccgacctc ggccaggaac cggtggttgc
     4981 gggcgatcga gaaggactgg aggagcacgt cgatggggcg ccggggctcg ggccgcggcc
     5041 gctcgaagtt cccgacgtgc atcaccacga cgcgcgtcgc tcccagctcg acggcccgcg
     5101 agatcgggac gttgttgacc acgccgccgt cgatgtacgg cgtgccgtcg atcaccacgg
     5161 gagggaagac agcgggcagg gccgccgagg ccagcaccgc ctcgtcgacc gaaccggtcg
     5221 cgaaccagtg ctccttgccg gtccgcagat cggtcgccac cacctcgacc gggatggccg
     5281 cctcctcgag caggtggtag gggagccatt ggcgcagcag gttgcgcagc ccgtcgttgg
     5341 ccgtgagcga cctggccttg cgggtgagca gccgtacgct cgacagccgg ctgctggggc
     5401 agagcatgtc gttgtggacg ccgagccaag cctcctggag ctggcgagcc ccttccgggg
     5461 tggggtcggc ggccagggcg gccgcgttga tggccccgac cgagcacccc acgaccacgt
     5521 cgggccggat gccgcgttcc agggcggcgc ggagcatgcc gacctggatg gcccccaggt
     5581 tcccgccccc gccgagcacg aagaccgtct ttgccttgga ccgcgt
//
LOCUS       DASWGG010000380         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_543462, whole genome shotgun sequence.
ACCESSION   DASWGG010000380 DASWGG010000000
VERSION     DASWGG010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543462"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VF954_06990"
     CDS             complement(<1..333)
                     /locus_tag="VF954_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530864.1"
                     /translation="MTLLTGTMSGGDVAAVVGSVLGVVVAVALLVFLVSVTRTLQAVR
                     ASVEELRRETLPILGELRTTVGSANAELVRVDTLLTSAESISSTVDSASRLAYLAFSN
                     PVIKTLAVG"
     gene            complement(498..2228)
                     /gene="aspS"
                     /locus_tag="VF954_06995"
     CDS             complement(498..2228)
                     /gene="aspS"
                     /locus_tag="VF954_06995"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX9530865.1"
                     /translation="MRTDHCGTLRPGDAGRAVTVCGWVARRREHGEHLAFIDVRDHTG
                     LVQCVVDGAHDLRSEYVVRVTGTVRARPEGTANPSLPTGGVEVGDCAVEVLSVAEPPP
                     FPVSDRSDADETVRLRHRYVDLRRDRLQRNLRMRATVNAALRAAMDAQGFAEVETPML
                     IASTPEGARDFVVPARLHPGSFYALPQSPQLFKQLLMVGGFDRYYQIARCLRDEDLRA
                     DRQFEFMQLDAEAAFVDQEDVLSFISPAVLAATEAATGTRVEGGPDGIPRLSWADAMA
                     RFGSDKPDVRFGMELVNLTPVFAATELRAFQAPAVKAIRVPGQGGVVRSRLDALTDTA
                     KRWGAKGLVWARVTGPGEIDSPVAKFLSAQEQAGVVAATAAEAGDLVLVVADERRQAE
                     KVLGLLRLELGRPPVNEGGYHFLWVTDFPLFEGLDDQGRPIPAHHPFTMPHPDDLDRL
                     EADPLSVRSQAYDLVLNGWELGSGSVRIHRPDVQQRIFDLIGIAPEAAQERFGFLLDA
                     FRYGAPPHAGFAFGIDRLVAILAGEENIREVIAFPKTQSGSDPLTGAPSPLEPAVLAE
                     LGIRVLPPPP"
     gene            complement(2234..>3079)
                     /locus_tag="VF954_07000"
     CDS             complement(2234..>3079)
                     /locus_tag="VF954_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX9530866.1"
                     /translation="DVEVITLAWDTFRQVGLTRTELLLNSMGDGTCRPGYRAALLAYL
                     EARQDQLCDEHRAHLRDNPLRVLDCKRAGCRRVTEAAPRMIDHLCGPCAAHFARVREG
                     LAGAGIEATIDTRLVRGQDYYTRTTFEFAGLALEAAQNGIGGGGRYDGMVGELGGPPT
                     PGIGFGLGIERILLAADAEAVFPPSGRGVDVFVVDVTDGEVARDLSLLLRRAGIATDR
                     AFDRRSMKAQMKAADRSGARFAVVVGPDERAGGAVAVRDLRGGGGQEQIAVADLVDHL
                     RKVLA"
BASE COUNT          418 a         1125 c         1153 g          383 t
ORIGIN      
        1 gccgacggcc agggtcttga tcaccgggtt ggagaaggcc aggtaggcca gccgggacgc
       61 cgagtcgacg gtggatgaga tggactcggc cgaggtcagc agcgtgtcca cccggaccag
      121 ctcggcgttg gccgagccga ccgtcgtccg cagctccccc aggatgggca gggtctcgcg
      181 ccgcagctcc tcgaccgagg cccgcacggc ctgcagggtg cgcgtcaccg acacgaggaa
      241 cacgagcagc gcgaccgcga ccaccacgcc caggaccgaa ccgacgacgg cggccacgtc
      301 gccgccgctc atggtgccgg tcagcagagt cattctcgcc gatccctctc tccggtgggc
      361 cgggcggccc gggcggcgac tttagtgcga tgccttctcc gaggcccggc cgaggcccca
      421 accccggcct cctccgcacg gcggggcccc aaccccgccg gcggccgtgc actccgaggc
      481 ccggccgagg cccccggcta cggcgggggc ggcaggacgc ggatgcccag ctcggccagg
      541 actgccggct ccagcggcga cggcgcgccg gtgagcggat cggaccccga ctgcgtcttg
      601 gggaaggcga tcacctcccg gatgttctcc tcccccgcca ggatggccac caggcggtcg
      661 atcccgaagg cgaacccggc gtgcggcggc gccccgtacc ggaaggcgtc caggaggaag
      721 ccgaatcgct cctgggccgc ctccggggcg atgccgatca gatcgaagat gcgctgctgc
      781 acgtccgggc ggtggatccg cacgctgccc gagccgagct cccagccgtt cagcaccagg
      841 tcgtaggcct gggagcgcac gctcaggggg tcggcttcca gccggtccag gtcgtcgggg
      901 tgcggcatgg tgaaggggtg gtgggcgggg atgggccggc cctggtcgtc gaggccctcg
      961 aagaggggga agtcggtcac ccacaggaag tggtagccgc cctcgttgac tggcgggcgc
     1021 ccgagctcca ggcgcagcag gccgagcacc ttctcggcct gccggcgctc gtcggccacc
     1081 accaggacca ggtcgccggc ctcggccgcc gtggcggcga cgaccccggc ctgctcctgg
     1141 gcggagagga acttggcgac cggagagtcg atctcgcccg ggccggtgac ccgcgcccag
     1201 acgagccctt tggcgcccca ccgcttcgcc gtgtcggtca gggcgtccag gcgtgacctg
     1261 accacgccgc cctggccggg cacgcggatg gccttcaccg cgggggcctg gaaggcgcgg
     1321 agctccgtgg ccgcgaacac cggggtgagg ttgaccagct ccatcccgaa gcgcacgtcg
     1381 ggcttgtccg agccgaaacg ggccatggcg tcggcccacg acaggcgggg gatgccgtcc
     1441 ggccccccct cgacgcgggt cccggtggcg gcctcggtgg cggccagcac ggcgggcgag
     1501 atgaaggaca ggacgtcttc ctggtcgacg aaggccgcct cggcgtcgag ctgcatgaac
     1561 tcgaactggc ggtcggcccg gaggtcctcg tcgcggaggc agcgggcgat ctggtagtag
     1621 cggtcgaagc cgccgaccat cagcagctgc ttgaacagct gcgggctctg gggcagggcg
     1681 tagaacgagc ccgggtggag gcgggccggc accacgaagt cgcgcgcccc ttccggcgtg
     1741 gaggcgatga gcatgggcgt ctcgacttcg gcgaagccct gggcgtccat ggcggcgcgc
     1801 agggcggcgt tgaccgtggc gcgcatccgc aggttgcgct ggagccggtc ccggcgcagg
     1861 tcgacgtagc ggtggcgcag cctcacggtc tcgtccgcgt cgctgcggtc gctcacgggg
     1921 aacggcggcg gctcggcgac cgagaggacc tcgacggcgc agtcgccgac ttcgacgccg
     1981 cccgtcggca ggctcgggtt cgccgtgccc tcgggccgcg cccgcacggt gcccgtgacc
     2041 cgcacgacat actccgagcg caggtcgtgg gcgccgtcga cgacgcactg gaccaggccg
     2101 gtgtgatcac gcacgtcgat gaaggccagg tgctcgccgt gctcccggcg ccgggcgacc
     2161 cacccgcaca cggtcacggc ccggccggcg tcgcccggcc gcagggtgcc gcagtggtcg
     2221 gtacgcatcg ggctcacgcc aggaccttcc gcaggtggtc gacgaggtcg gccacggcga
     2281 tctgctcctg gccgcccccg ccccgcaggt cccgaacggc cacggcgccc ccggcccgct
     2341 cgtccgggcc caccaccacc gcgaaccggg cgccggagcg gtcggcggcc ttcatctggg
     2401 ccttcatgga ccgccggtcg aaggcgcggt cggtggcgat gccggcccgc ctgagcagca
     2461 acgacaggtc gcgggccacc tcgccgtcgg tcacgtcgac gacgaagacg tcgacgcctc
     2521 ggcccgacgg cggaaagacg gcttcggcgt cggcggcgag caggatgcgc tcgatcccga
     2581 gcccgaagcc gatcccgggc gtgggcgggc cgcccagctc gcccaccatc ccgtcgtagc
     2641 ggccgccacc gccgatgccg ttctgggccg cctccagggc cagcccggcg aattcgaagg
     2701 tggtcctggt gtagtagtcc tggccccgca ccaggcgggt gtcgatcgtc gcctcgatcc
     2761 ccgccccggc gagaccctcc cggaccctgg cgaagtgggc ggcgcaaggc ccgcagaggt
     2821 ggtcgatcat ccggggcgcg gcctcggtga cccggcggca gccggcccgc ttgcagtcca
     2881 gcacgcgcaa cgggttgtcg cgcagatgcg cccggtgctc gtcgcagagc tggtcctggc
     2941 gcgcctccag gtaggccagc agggctgcgc ggtagccggg gcggcacgtg ccgtcgccca
     3001 tggagttgag cagcagctcc gtgcgggtga gccccacctg gcggaaggtg tcccacgcca
     3061 gggtgatcac ctcgacgtc
//
LOCUS       DASWGG010000381         3240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2149773, whole genome shotgun sequence.
ACCESSION   DASWGG010000381 DASWGG010000000
VERSION     DASWGG010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2149773"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1297
                     /locus_tag="VF954_07005"
     CDS             191..1297
                     /locus_tag="VF954_07005"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEX9530867.1"
                     /translation="MRWARAVLGTLMAGVVAWQAGPAGACGGLVAPNGAVRLVRTTTL
                     AAWHAGYEHYVTSFQFDGDGAEFGSIVPLPGIPTKVEKGGSWTLQRLEREVQPPVATA
                     AASSGVGEAGSATVILQTHVDALELTVLKGGGGAVGQWAKANGFLLTPDAPELLDYYA
                     RHSLIFLAARFDAAQARARGQQFGDGTPVHLTIPLPKPWVPLRILTLGQRPSDPVSAD
                     VFVLTDLRPTIHLPEVGARVARSQPASSQLLGDLRADQGMQWVPDRMWLTYVAIDAPA
                     LQINADLVVAGPPRPPARSARPTPRPPTTLAPTTTSTAPTTAPPTTAGPVLDAAPLLA
                     GPAHRTTEPSGGAVLVALLLLALCGGGVARTLRR"
     gene            1294..1716
                     /locus_tag="VF954_07010"
     CDS             1294..1716
                     /locus_tag="VF954_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9530868.1"
                     /translation="MKAGASISGAVMFLLLGVRPAAAPAPIGGPGRIVTVRITIHHSA
                     FSPRHLRVADGSLVAFHVTNTDPIDHELIVGDQATQDRVERGDEPDHHARGQVSVAAG
                     ASASTYYVFGGQRWWQFGCHLPGHWAYGMRGTIEVARR"
     gene            complement(1760..>3240)
                     /locus_tag="VF954_07015"
     CDS             complement(1760..>3240)
                     /locus_tag="VF954_07015"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX9530869.1"
                     /translation="PLGDTVPRDVLTPSPRLVSETLLAREVFRPATTLNVLAAAWLQF
                     EIHDWLTHRNGSSKDPWVVPLETTDPWPHPPMQVRRTPRTSGDDRVPSFANTATHWWD
                     GSQLYGSSADRQRPLRTHEGGKMLIGPNGVVPYDPATGREMAGVVGNWWVGLGLMHSI
                     FLREHNAICDRLAGAYPGWSDQELFDHARLVNAALMAKIQTVEWSTAVVASTTMRTAL
                     RANWWGLAGEWWTEHFGRIDAHDEVSGVPGAEADLFGVPYSMTEEFVSVYRMHPLIPD
                     DFSLRSARDDSLIEERSLPQMVFSEARPVLERVPVADLLYSLGTRHPGVIGLHNTSRY
                     LRELRRPDGELIDLAAVDVLRDRERGVPRYNEFRRRFRLDPARTFADFSSDEQVVEQL
                     ARVYDSPEDVDALVGMFAEAPPPGFAFSDTAFRVFILMATRRLKADRFFTYDYRPEVY
                     TEEGLEWVRDNTMRSVLLRHHPELEPALRSVDNPFKPWNTIG"
BASE COUNT          445 a         1167 c         1165 g          463 t
ORIGIN      
        1 ggcggcggcc gccgccagga ccgcgacgtc gtcacccgcc gcgtcccgca cagcgtgtct
       61 ggtcatgagc gtcccatcag tgccccattg gtgcgatggc ctcgccgaaa tcaatcgccg
      121 gttgatcacg tccggcccac cgcactaacc gccactgagc ggccttggag ggagacccac
      181 ggtgagccgg atgcgatggg ccagagcggt gctggggaca ttgatggcag gcgtcgtggc
      241 ctggcaggct ggtccggcgg gtgcgtgcgg cgggctggtg gctcccaacg gggccgtgcg
      301 cctggtgcgc acgaccacgc tggccgcctg gcatgccggc tacgagcatt acgtcacatc
      361 gttccagttc gacggggacg gggccgagtt cggctcgatc gtgccccttc cgggcatccc
      421 caccaaggtg gagaagggcg ggtcgtggac ccttcagcgc ctcgagcggg aggtccagcc
      481 gcccgtcgcg acagccgctg catcgagcgg ggtcggcgaa gccggcagcg ccacggtcat
      541 cctccagacc cacgtggatg ccctcgagct cacggtcctc aaggggggcg gcggcgcagt
      601 cggacagtgg gccaaggcga acggcttcct tctgacgccg gacgcgcccg agctcctcga
      661 ctactacgcc cggcacagcc tgatcttcct ggcggcgcgc ttcgacgccg cccaggcgcg
      721 ggcgcggggg cagcagttcg gggacggcac accggtccac ctgaccatcc cgctgcccaa
      781 gccctgggtg cccttgcgca tcctgacctt ggggcaacgc ccgtccgacc cggtcagcgc
      841 cgacgtattc gtgctgaccg atctccgccc gaccatccac ctgcccgagg tcggggcccg
      901 agtggcccgc agccagccgg catccagcca gctgctcggg gacctgcgcg ccgaccaggg
      961 catgcaatgg gtgccggatc gcatgtggct gacctacgtg gccatcgacg ccccggcgct
     1021 ccagatcaac gccgacctgg tcgtcgcggg gcccccgagg ccgccggccc gatccgcccg
     1081 gccgaccccc cgccctccga ccacgctcgc gccgaccacg accagcaccg cgcccaccac
     1141 ggcgcccccc accacggccg ggccggtgct cgacgccgcg ccgctgctcg ccggcccggc
     1201 ccaccgcacc acggagccat ccggcggcgc ggtgctggtc gccttgctcc tgctggcgct
     1261 gtgcggcggg ggtgtcgccc gcaccctgcg gcggtgaagg cgggcgcgtc gatcagcggc
     1321 gccgtcatgt tcctcctgct cggcgtgcgg cccgccgccg cgccggctcc gatcggcggt
     1381 cccggccgca tcgtgacggt gcggatcacc attcaccaca gcgccttctc gccgcgccac
     1441 ctccgggtcg ccgacggctc cctcgtcgcc ttccacgtga ccaacaccga ccccatcgac
     1501 catgagctca tcgtgggcga ccaggcgacc caggaccggg tggagagagg tgacgagccc
     1561 gaccatcacg cccgcgggca ggtctcggtg gccgctggcg ccagcgcgtc cacgtactac
     1621 gtgttcggcg gccagcgatg gtggcagttc ggctgccacc tgcccggcca ctgggcctac
     1681 gggatgcggg gcaccatcga ggtcgcgcgc cggtaggccg gtggtccggt ccgccgccgc
     1741 ccggggcgtc gggaccggct cagccgattg tgttccaggg cttgaacggg ttgtccaccg
     1801 agcgcagggc gggctccagc tccgggtggt ggcggagcag gacgctgcgc atggtgttgt
     1861 cgcggaccca ttccaggcct tcttcggtgt acacctcggg ccggtagtcg taggtgaaga
     1921 accggtcggc cttgagccgc ctggtcgcca tgagtatgaa cacgcggaac gcggtgtcgc
     1981 tgaacgcgaa gcccggcggc ggcgcctcgg cgaacatccc caccagggcg tcgacgtcct
     2041 cgggggagtc gtacacgcga gcgagctgct cgaccacctg ctcgtcggag ctgaagtctg
     2101 cgaacgtccg ggcggggtcg aggcggaacc gcctgcggaa ctcgttgtag cgcggcaccc
     2161 cccgctcgcg gtcgcgcagc acgtcgacgg ctgccaggtc gatcagctcg ccgtcgggcc
     2221 ggcgaagctc ccgcaggtac ctggaggtgt tgtgcagccc gatgacaccc gggtgcctgg
     2281 taccgagcga gtagagcagg tcggccacgg gcacgcgctc gaggacgggg cgggcctcgg
     2341 agaacaccat ctggggaagg ctgcgctcct cgatcaagct gtcgtcgcgc gccgagcgga
     2401 ggctgaagtc gtcggggatg agggggtgca tgcggtagac ggagacgaac tcctcggtca
     2461 tcgagtacgg cacaccgaac agatcggcct cggccccggg cacgccgctg acctcgtcgt
     2521 gtgcgtcgat gcgtccgaaa tgctccgtcc accactcgcc ggccaggccc caccagttgg
     2581 ccctgagggc ggtgcgcatg gtcgtgctgg ccacgaccgc ggtcgaccac tcgaccgtct
     2641 ggatcttggc catcagcgcg gcgttgacca gccgggcgtg gtcgaagagc tcctggtccg
     2701 accagcccgg gtaggcgccg gcgaggcggt cgcagatggc gttgtgctcc ctcaggaaga
     2761 tcgagtgcat gaggcccagc ccgacccacc agttgcccac cacgccggcc atctctcgcc
     2821 cggtggcagg gtcgtagggc accacgccgt tggggccgat gagcatcttg cccccctcgt
     2881 gggtgcggag cgggcgctgg cgatccgcgc tgctgccgta gagctgggag ccgtcccacc
     2941 agtgcgtcgc ggtgttggcg aacgacggca cccggtcgtc gcccgaggtc cgcggcgtgc
     3001 gccgcacctg catcgggggg tggggccacg ggtcggtcgt ctccagggga acgacccagg
     3061 ggtccttgga gctcccgttg cggtgggtca accagtcgtg gatctcgaac tggagccatg
     3121 ccgcggccag gacgttgagc gtggtcgccg ggcggaacac ctcccgggcg aggagcgtct
     3181 cgctcaccag ccgcgggctc ggggtgagga cgtcgcgggg cacggtgtcg cccaggggaa
//
LOCUS       DASWGG010000382         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_217766, whole genome shotgun sequence.
ACCESSION   DASWGG010000382 DASWGG010000000
VERSION     DASWGG010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217766"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /gene="cbiD"
                     /locus_tag="VF954_07020"
     CDS             complement(<1..606)
                     /gene="cbiD"
                     /locus_tag="VF954_07020"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HEX9530870.1"
                     /translation="MSGPPVRPAPHEPPLAPEVARATGLRTGFTTGTCAAAAAKAAVT
                     WLATGNCPPQAEVALPGGRRVSFPVERGPAVHQCAVVKDAGDDPDVTHGAHVTALAAW
                     LGGDPAGTVELLAGDGVGTVTLPGLGLPVGAPSVTSVPRAMIGSAVAEVTDRPVRLTL
                     SVPGGEAMAARTSNARLGIAGGISILGTTGIVRPFSTAAWRA"
     gene            complement(603..1421)
                     /gene="cobM"
                     /locus_tag="VF954_07025"
     CDS             complement(603..1421)
                     /gene="cobM"
                     /locus_tag="VF954_07025"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HEX9530871.1"
                     /translation="MISFVGAGPGAPDLITLRGAERLAAADVVIWASSLVPAAVLDHC
                     RPGIEVHDSATMTLEDVVAVYARHGPDTPIVRLHSGDPSLYGALTEQLDWCRANSRPW
                     EMVPGVSSLGAAAAALGRELTVPGLSQSVVVTRLSGRTAASMPAGEGVAAYAAHGGTL
                     AVFLSAARPDELQAELLAPGSAYLPETPAAIVVRASWPDERIVRTTVGHLARDLRASG
                     ATMTALVLVGAALASGDAGASRTHLYSPAFTTAYRLRSRAGSTGGRPSARRPGP"
BASE COUNT          194 a          539 c          623 g          147 t
ORIGIN      
        1 ggcccgccag gccgcggtgg agaaggggcg gacgatgccg gtcgtgccca ggatggagat
       61 gccgccggcg atgcccaggc gggcgttgga ggtccgggcc gccatggcct cgccgcccgg
      121 gaccgacagg gtgagccgga cggggcggtc ggtgacctcg gcgacggccg agccgatcat
      181 ggcccggggc acggaggtca cggacggcgc gcccaccggc aagcccagtc cgggcagcgt
      241 taccgtgccg accccgtcgc cggcgagcag ctcgacggtg ccggcgggat cgccgcccag
      301 ccaggccgcc agcgcagtga cgtgggcccc gtgggtgacg tcggggtcgt cgccggcgtc
      361 cttgacgaca gcgcactggt ggaccgccgg gccccgttcc accgggaagg agacgcgccg
      421 gccgccgggc agcgccacct cggcctgggg cgggcagttc ccggtggcga gccacgtcac
      481 cgcggccttg gccgccgcgg ccgcgcacgt gccggtggtg aagcccgtcc gcagtcccgt
      541 ggcccgggcc acctcgggag cgaggggcgg ctcgtggggc gccggccgca caggggggcc
      601 gctcacgggc ccggccggcg ggccgacggc cgacccccgg tgctgccggc gcgggagcgc
      661 agccggtagg cggtcgtgaa ggccggggag tagaggtggg ttcgagaggc gccggcgtcg
      721 ccgctcgcga gcgccgcgcc cacgaggacc agggcggtca tggtcgcgcc gctggcccgg
      781 agatcccggg ccaggtggcc gaccgtggtc cgaactatcc gctcgtccgg ccacgacgcc
      841 cgcacgacga tggccgcggg ggtctcggga aggtaggcgg agccgggcgc cagcagctcg
      901 gcctgaagct cgtcggggcg cgccgcagac aggaagacgg cgagcgtccc gccgtgggcc
      961 gcgtaggccg ccacgccctc gcccgccggc atcgaagccg cggtgcgacc ggacagccgg
     1021 gtgaccacga cgctctggga gaggccgggc acggtgagct cgcggcccag cgcggccgcc
     1081 gccgccccga gggacgacac gccgggcacc atctcccagg gacgggagtt ggcccggcac
     1141 cagtcgagct gctccgtcag tgcgccgtac agcgagggat cgcctgagtg cagccgcacg
     1201 atgggcgtgt ccgggccgtg gcgggcatac acggcgacca cgtcctcgag ggtcatggtg
     1261 gccgagtcgt ggacctcgat gcccgggcgg cagtggtcga ggacggcggc gggcacgagg
     1321 gatgaggccc agatcaccac gtcggcggcg gccaggcgtt ccgcgccgcg cagggtgatg
     1381 aggtcggggg cgcccggccc ggcaccgacg aagctgatca cgcgcccccc tcctcggcgg
     1441 ggacgatcac agtggccagg tacgacgccg ggccgtgggc cagggcctcg agggcgccga
     1501 ccc
//
LOCUS       DASWGG010000383         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_548173, whole genome shotgun sequence.
ACCESSION   DASWGG010000383 DASWGG010000000
VERSION     DASWGG010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548173"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VF954_07030"
     CDS             <1..804
                     /locus_tag="VF954_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9530872.1"
                     /translation="TDAGPPDGEVVVLLHGYPETRQSWDRVVPLLVDAGHRVLAPDQR
                     GYSPGARPRGRPAYALNHLVNDAIALIDASGADKVHLVGHDWGGAVAWATAAWHPERL
                     YSMTSLATPHRNALLRSLVTSTQALHSWYMLFYQLPWLPELSLKGPAFRLALQRSGLP
                     EADIARYLAVLSQPGAATAAVNWYRALPFTPPSRMGPVSVPVLYIYGTGDFALGRKAA
                     DLTGSYVTGPYRYEVLEGISHWIPEQVPDVVAGLLLEQLSAYGAAAPSS"
     gene            complement(860..>1662)
                     /locus_tag="VF954_07035"
     CDS             complement(860..>1662)
                     /locus_tag="VF954_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9530873.1"
                     /translation="YGAGSMAVWDTGTYDEEKWSDREVMVQFHGRRVEGRYVLFRTGG
                     KSWMIHRMDPPTDPERQPPPSGVAPMWARPAPRLPGGAAHAWAADVAWGGARILLTVQ
                     GGRPLALHWDGPGRDPQEVTDRFPELRAVAATVGATELLVDGELVVTGADGRPDDGRL
                     ADRLGASGASVRRLAAAHPAVVMVADALWIDGHTLLARPLRERRSRLLEVRPGHPAWQ
                     AQPLRAGEVRALRDAARRQRLPGVVAKRVESAYEPGRVSEGWRMVVSA"
BASE COUNT          206 a          624 c          601 g          231 t
ORIGIN      
        1 accgacgcgg ggccgcccga tggggaagtc gtcgtcctgc tccacggcta tcccgaaacg
       61 cgccagtcgt gggatcgggt ggtcccgctg ctcgtggacg ccggccaccg cgtgctggct
      121 cccgaccaga gggggtactc accgggagcg cggccccggg gtcggccggc ctacgcgcta
      181 aatcacctgg tcaatgacgc gatcgcgctc atcgacgcat ccggcgcgga caaggtgcac
      241 ctcgtggggc acgactgggg cggcgctgtt gcctgggcga cggctgcgtg gcaccccgag
      301 cggctgtaca gcatgacctc gctcgccacg ccgcaccgca acgcgctgct ccgctctctg
      361 gtgacgagca ctcaggcatt gcactcctgg tacatgctct tctaccagct cccctggttg
      421 ccggagctga gcttgaaagg gccggcgttc cgtctggcac tccagcgttc cgggctcccg
      481 gaggccgaca tcgcccggta cctggcggtg ctgtcccagc ccggcgcggc caccgcggcg
      541 gtcaactggt accgggcgct gccgttcacg cctccctcac ggatggggcc ggtcagcgtg
      601 ccggtgttgt acatctacgg cacgggtgac ttcgcgttgg ggcgaaaggc cgccgacctg
      661 accgggtcct acgtcacggg cccgtaccgg tacgaggtgc tcgaagggat cagccactgg
      721 atccccgagc aggtgccgga cgtcgtcgcc gggcttctgc tcgaacagct cagcgcgtac
      781 ggagccgcgg cacccagctc ctgacccctg ggcgggacgg gtccgggcgt ggtgggtgca
      841 gcccctcggt ggcggcccgt caggcgctca cgaccatccg ccacccctcg ctcacccggc
      901 cgggctcgta ggcggactcg accctcttcg ccaccacgcc ggggaggcgt tggcggcgcg
      961 ccgcatcccg cagggccctg acctccccgg cccgcagtgg ttgggcttgc caggcgggat
     1021 ggcctgggcg gacctcgagg aggcgggacc tgcgctcacg gaggggccgc gccagcagtg
     1081 tgtggccgtc gatccagagg gcgtcggcga ccatgacgac ggcggggtgg gcggcggcga
     1141 gccgacggac cgatgcgccg ctcgcgccga gccggtccgc cagccggccg tcgtcggggc
     1201 ggccgtcggc gccggtcacg accagctcgc cgtccaccag cagctcggtc gcaccgaccg
     1261 tcgcggcgac ggcccgcagc tccgggaacc ggtcagtcac ctcctgcggg tcgcggccgg
     1321 gcccgtccca gtgcagggcc aggggccggc cgccctgcac cgtgagaagg atgcgggccc
     1381 cgccccacgc gacgtcggcg gcccaggcgt gcgcggcgcc ccccggcagc cggggcgccg
     1441 gccgggccca catgggcgcc actccggagg gcgggggctg gcgctccggg tctgtgggcg
     1501 ggtccatccg gtggatcatc cagctcttgc cccccgtgcg gaacagcacg tagcgcccct
     1561 cgacgcgccg gccgtggaac tggaccatca cctcccggtc gctccacttc tcctcgtcat
     1621 aggtgccggt gtcccacacg gccatcgagc ctgcgccgta gc
//
LOCUS       DASWGG010000384         2598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_219787, whole genome shotgun sequence.
ACCESSION   DASWGG010000384 DASWGG010000000
VERSION     DASWGG010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219787"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VF954_07040"
     CDS             complement(<1..376)
                     /locus_tag="VF954_07040"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX9530874.1"
                     /translation="MTTGTEHTPLGTGSLDGLVVGITADRRAVEQADLLRRMGAQIVH
                     GPTISTSAVGDQDRARQATQDVIARPPDVLLATTGIGMRSWMVQAETEGLDDALLCAL
                     ARTRVAARGPKAAGAVRQLGLEI"
     gene            complement(770..1036)
                     /locus_tag="VF954_07045"
     CDS             complement(770..1036)
                     /locus_tag="VF954_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530875.1"
                     /translation="MTKRARKSTGRSGSRRPATNAFWGEIDLEPPPRIRPTTDPGAVP
                     RSLGTPPLSAPPQAVQGQLQIVYAEAVRAATALAAAAGLLEDDL"
     gene            complement(1033..>2598)
                     /locus_tag="VF954_07050"
     CDS             complement(1033..>2598)
                     /locus_tag="VF954_07050"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9530876.1"
                     /translation="SALAGSVTVDGSGWAADLLRSVQSAAVEPKAAPASFVGRLREYQ
                     AEALGWLYLLDRVGLGGCLALDMGLGKTPTVLALIAGAKEGTAPTLVITPPAVLGNWE
                     VEAKRFTPGLRVVIHHGASRAGEDTLARVAGRANIVLTTYGTAVRDMDALAKIEWDHV
                     VIDEAQAIKNPVSETAQALRRLQARTRLALTCTPVENGLGDLWSILDFTNPGLVGPRN
                     AFIEQLSKVSGGAGAEGALRALGGLLVFRRTKAEPAIAAELPDKIDQLDHCSMTAEQI
                     GLYQAILDRLLGDGLSEDKNERKGQVLAAITALKQVCDHPAAYLHDDDGVVGGRSGKL
                     ARLDEIVEAVFAAGERLLVFTHFARWGQKLATHLTARTKHPVACYHGGLSRTERDRII
                     AEFQAGTGPGALVLSLKAGGSGLNLTNASHVVLYDRWWNPAVEDQARDRAWRIGQTKT
                     VVCHRLVCPGTVDERVEEVVAGKRRIADLALPASSSLADLTADQLRGALGIRDDALLD
                     EEEDHEVGEEDAA"
BASE COUNT          344 a          940 c          914 g          400 t
ORIGIN      
        1 agatctcgag gcccagctgg cggaccgccc ccgcggcctt cggtccccgg gccgccaccc
       61 tcgtgcgggc cagggcgcag agcaacgcgt cgtccaggcc ctcggtctcg gcctgcacca
      121 tccagctccg catcccgatc ccggtcgtgg ccagcaggac gtccggcggg cgggcgatga
      181 catcttgggt ggcctggcgc gcccggtcct gatccccgac ggcggaggtg ctgatggtcg
      241 gcccatgcac gatctgcgca cccatgcgcc gcaacaggtc cgcctgctcg acggcgcggc
      301 ggtcggcggt gatcccgacg accaggccgt cgagcgaacc ggtcccgagc ggcgtgtgct
      361 cggtgcccgt ggtcacgcgc ccacgttggc cggagcgcat ttcgctccgg ttgcgccccg
      421 ccgaaaggtg cgagacaagt tcctgaccgc aacgtggcgc cgctcagggt cgaacgcaaa
      481 gctacttggg cgctcgaaga ccattttcct tgtcgcgacg ggcgaggttc ttgagaaccg
      541 cccaagcagt tttgcgcttg accctcaccg acaacgggcc ccgaggtcgc agtcgggggg
      601 cctttgggcc attcttcaat gctttaatgt cgtcccagcg ccttttttgc ttgaagaatg
      661 atcttcgcgc taccggggct gggcgggtct ccgccgtgac gacataccca ccgtgacgac
      721 atacccaccg tgacggccta ccaccgtgac ggcctaccac cgtgacggcc tagaggtcgt
      781 cctcgagcag cccggctgcg gccgccaggg cggtggcggc cctcaccgcc tcggcgtaca
      841 cgatctgcag ctggccctgc acggcctggg gtggcgccga caggggcggc gtgcccaggg
      901 agcggggcac cgcgcccggg tcggtggtcg gacggatgcg gggcggcggc tcgagatcga
      961 tctcgcccca gaaggcgttg gttgcgggcc ggcgggaacc ggaccggccg gtcgacttgc
     1021 gcgcccgttt ggtcatgccg cgtcctcctc gccgacttcg tggtcctctt cctcgtccag
     1081 gagggcgtcg tcgcggatgc ccagcgcgcc gcgcagctgg tcggcggtca gatcggccag
     1141 gctgctgctg gccggcaggg ctagatccgc gatgcggcgc ttgccggcga ccacctcctc
     1201 gacccgctcg tcgaccgtgc cggggcagac caggcggtgg cacaccacgg tcttggtctg
     1261 gccgatccgc caggcgcggt cgcgggcctg gtcctcgacg gcggggttcc accaccggtc
     1321 gtacagcacc acgtggctgg cgttggtcag gttcaggccc gacccgcccg ccttcagcga
     1381 gagcaccagc gcgcccggac cggtgcccgc ctggaactcg gcgatgatgc ggtcgcgctc
     1441 ggtccgtgac agcccgccgt ggtagcaggc caccgggtgc ttggtccggg ccgtgagatg
     1501 ggtggcgagc ttctggcccc acctcgcgaa gtgtgtgaag accagcagcc tctcgccggc
     1561 ggcgaagacc gcctcgacga tctcgtcgag gcgagccagc ttgcccgagc ggccgcccac
     1621 gacgccgtcg tcgtcgtgca ggtaggcagc cgggtggtcg cagacctgct tcagggcggt
     1681 gatggcggcc agcacctggc ccttgcgctc gttcttgtcc tccgacaggc cgtcgcccag
     1741 caaccggtcg aggatggcct ggtacagacc gatctgctcg gcggtcatgc tgcagtggtc
     1801 gagctggtcg atcttgtcgg gcagctcggc cgcgatggcg ggctcggcct tggtccgccg
     1861 gaacacgagc aggcccccga gggcgcgcaa ggcgccttcc gccccggccc ctcccgacac
     1921 cttggagagc tgctcgatga aggcgttgcg cggcccgacc aggccggggt tggtgaagtc
     1981 gaggatcgac cagaggtcgc cgagcccgtt ctcgactgga gtgcacgtca gggccaggcg
     2041 ggtgcgcgcc tggagccggc gcaaggcctg agccgtctcg ctgaccgggt tcttgatggc
     2101 ctgggcctcg tcgatgacga cgtggtccca ctcgatcttg gccagggcgt ccatgtcccg
     2161 gacggccgtg ccgtaggtgg tgagcacgat gttggcccgg cccgccaccc gggccagggt
     2221 gtcctccccg gcccggctgg ccccgtggtg gatgaccacg cgcagcccgg gcgtgaagcg
     2281 cttcgcctcc acctcccaat tcccgaggac ggcgggcggc gtgatgacga gggtgggcgc
     2341 ggtgccctcc ttcgccccgg ctatgagagc cagcaccgtc ggcgtcttcc ccaaacccat
     2401 gtccagggcc agacagcccc cgagacccac ccggtcgagc aggtacagcc agcccagcgc
     2461 ctcggcctgg tactcccgca gccgccccac gaacgacgcc ggagccgcct tcggctccac
     2521 cgccgcgctc tgcaccgagc gcagcagatc ggccgcccag ccgctgccgt ccacggtgac
     2581 gctgccggcc agggcgga
//
LOCUS       DASWGG010000385         3608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_548802, whole genome shotgun sequence.
ACCESSION   DASWGG010000385 DASWGG010000000
VERSION     DASWGG010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548802"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VF954_07055"
     CDS             <1..612
                     /locus_tag="VF954_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530877.1"
                     /translation="TDAATICAARDAGAIDQLTRVLGAHPDGIDQANALLARAVEPLH
                     PAGRPLYAGLRSLGLPGSAVGDAWRRADMLREFRGDAHTAAWTSAGFDATEIGMLSEP
                     YMGLPLRSYVRSRGWSEAELDGAEVRLTDRGLLEGGALTDRGREEREAVEVATDRQCR
                     PFIDALGDDAGELISILAPWGSAIRAAKGYLASGPHDLASAFA"
     gene            complement(645..1679)
                     /locus_tag="VF954_07060"
     CDS             complement(645..1679)
                     /locus_tag="VF954_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX9530878.1"
                     /translation="MGAVADAEVPAGIDAGPVTEWFDAHVGGAVAPLAFARVAGGHSC
                     LTYVVLDAAGSRFVLRRPPLGHVLASAHDVVREHRIMAALEGTAVPVPRMLGVCPDGE
                     VNGAPFYVMSFVDGPVLHTAEDARERLPSDAARRRAAETLVDALVALHALDPDEVGLG
                     QLSRRSGYLDRQLKRWSAQWEASRTRDLPAMERLHAWLVEHRPAESSARIVHGDYRLG
                     NCVHDPTDGRVLAMLDWELCTLGEALADVSYLLRSWSGGEGVSSAPGFPGWEEVAARY
                     AAGSGRSIADLDYWMAFNAWRSAAIAEGVYRRYLDGNMGEAPEGVDGYARGVEMSVRA
                     GLQAAALDPG"
     gene            1820..2932
                     /locus_tag="VF954_07065"
     CDS             1820..2932
                     /locus_tag="VF954_07065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9530879.1"
                     /translation="MKLGLLYEIQIPKPWTPGIEARTYGEAMEQIVLADQAGWAYVWA
                     VEHHLLPQWSHCSAPEVFLGAVSQRTTRIRIGHGIALLPRGFNHALRVAERAAVLDIL
                     SNGRVELGTGRAVTLQELDGFEIAPDQTMPQWAEAIDIIVRAWRDEPVEHHGQFYDIP
                     PSFVVPKPVQDPHPPLWLAGTNPETFERAGRSGLGVLGFVTGKPADVQPRIDAYKAAV
                     AGATPVGDALNDNAAVLMQTYCAETREQAIADVEQALGKVAQLAVDLFLPWAEQPAER
                     NLESYRYLTRPAAAGDTRAGAYFGDTQERVRDGMIAVGTPDDLVDLFGRYDRMGVDQM
                     LTWVQFGGLPHDRIMRSMELIAERVLPQLEPARQAP"
     gene            2929..>3608
                     /locus_tag="VF954_07070"
     CDS             2929..>3608
                     /locus_tag="VF954_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530880.1"
                     /translation="MTAGAEDVAVLRRTAIAGLGITDVGKVYGRSPGDFAADAARRAV
                     ADAGLEMGDVDGLLLSNGISGGVDLRLQRELGLHDLRLLAQMTAAGATAIAQVQYAAL
                     AVASGLASTVVCVHADAPLRPMESSGATYRWGGGAGRGPAGVDALSFVGGMRTANSGY
                     ALAARRHMARYGTTSEQLGALAVAQRGWAAKNPIARFRTPITVEDHQASRWVVEPLHL
                     LDCCMVSNG"
BASE COUNT          503 a         1361 c         1309 g          435 t
ORIGIN      
        1 accgacgcgg ccacgatctg cgcggcccgg gacgcagggg ccatcgacca gctcacccgg
       61 gtgctcggcg cgcacccgga cggcatcgac caggccaacg ccctgctcgc ccgggcggtc
      121 gagcccctcc acccggccgg caggcccctc tacgcggggc tacggtcgct cgggctgccg
      181 ggcagcgccg tcggtgacgc gtggcggcga gccgacatgc tcagggagtt ccggggcgac
      241 gcccataccg cggcctggac ctcggccggc ttcgacgcca ccgagatcgg catgctctcg
      301 gagccctaca tgggcctgcc gctgcgctcc tatgtccgga gccggggatg gagcgaagcc
      361 gagctagacg gggccgaggt ccggctgacc gaccggggcc tgctcgaggg cggggcgctc
      421 accgaccggg gccgggagga gcgcgaggcg gtcgaggtgg cgacggatcg ccagtgccgg
      481 ccgttcatcg acgccctcgg ggacgacgcg ggcgagctga tctcgatcct cgccccgtgg
      541 ggttccgcca tccgcgccgc caagggctat ctcgcctccg ggccccacga cctggcatcc
      601 gccttcgcgt gacgccgcgg tcgggtcacc ggacgggccc gccgtcagcc cgggtcgagg
      661 gcggcggcct ggagcccggc tcgcacgctc atctcgacgc cgcgggcata cccgtcgact
      721 ccctcgggcg cctcacccat gttcccgtcg aggtagcggc ggtacacccc ttcggcgatg
      781 gccgccgagc gccacgcgtt gaacgccatc cagtagtcga ggtcggcgat gctgcgcccc
      841 gagcccgccg cgtaccgggc tgccacctcc tcccagccgg gaaagccggg cgccgacgag
      901 acgccctctc cgccgctcca ggagcgcagc aggtacgaca cgtcggccag ggcctcgccg
      961 agggtgcaca gctcccagtc gagcatggcc agcacccggc catcggtcgg gtcgtgcacg
     1021 cagttgccga gccggtagtc gccgtgcacg atccgcgccg acgactcggc gggtcggtgc
     1081 tcgaccagcc aggcgtgcag ccgctccatc gccggcaggt ccctggtccg cgacgcctcc
     1141 cactgcgccg accaccgctt gagctgacga tcgaggtacc cgctgcgccg ggacagctgg
     1201 cccaggccca cctcgtcggg gtcgagggcg tgcagggcca cgagggcgtc gacgagcgtc
     1261 tccgccgccc ggcgccgggc ggcgtcggac gggagccgct cgcgggcatc ctcggcggtg
     1321 tgcaggaccg gaccgtcgac gaagctcatc acgtagaacg gcgccccgtt gacctcgccg
     1381 tcggggcaca cgccgagcat ccgtggcacc ggcaccgccg tgccctcgag cgcggccatg
     1441 atccggtgct cgcggaccac gtcgtgggcg ctcgccagga cgtggccgag cggcggccgc
     1501 cgcagcacga accggctccc ggcggcgtcc agcacgacgt aggtgaggca ggagtggccc
     1561 ccggcgaccc gggcgaacgc cagcggcgcg accgcgcccc ccacgtgggc gtcgaaccac
     1621 tcggtcaccg ggccggcatc gatgccggca ggaacctcgg catcggcaac ggcccccacg
     1681 gggtgaacct accccccttc cgagcgggcc cgcacggcgg cgagcgggcc cgggcgggcc
     1741 aggtcggcct cgacacgcgg cgccgcgtcg agctaggaaa gccggcactg ggcgccggcg
     1801 ggagggaggg ggagcagcgg tgaagctagg gctgttgtac gagatccaga tcccgaagcc
     1861 gtggacgccg ggcatcgagg cccgcaccta cggcgaggcc atggagcaga tcgtgctggc
     1921 cgaccaggcc ggttgggcct acgtgtgggc cgtggagcac cacctccttc cccagtggtc
     1981 gcactgctcg gcccccgagg tcttcctcgg cgccgtctcc cagcgcacca cccgtatcag
     2041 gatcgggcac ggcatagccc tgttgccacg cggcttcaac cacgccctgc gggtggcgga
     2101 gcgggcggcc gtcctcgaca tcctcagcaa cggccgggtc gagctgggca ccggccgggc
     2161 ggtgaccctc caggagctcg acggcttcga gatcgcccct gaccagacca tgccgcagtg
     2221 ggccgaggcc atcgacatca tcgtgcgggc gtggcgggac gagccggtcg agcaccacgg
     2281 gcagttctac gacatcccgc cgagcttcgt cgtgcccaag ccggtgcagg atccccaccc
     2341 acccctgtgg ctggccggga ccaaccccga gaccttcgaa cgggccggcc ggagcgggct
     2401 cggtgtgctc ggcttcgtga ccggcaagcc ggccgacgtg cagccacgaa tcgacgccta
     2461 caaggccgcc gtcgccgggg ccacaccggt gggcgacgcg ctcaacgaca acgccgccgt
     2521 gctcatgcag acctattgcg ccgagacccg cgagcaggcg atcgccgacg tcgagcaggc
     2581 gttgggaaag gtggcccagc tggcggtgga cctgttcctc ccctgggcgg agcagccggc
     2641 cgaacgcaac ctggagtcat accgctacct gacccggccg gcggcggccg gcgacacccg
     2701 ggccggcgcc tatttcggcg acacgcagga gcgggtccgc gacggcatga tcgccgtcgg
     2761 cacgccggac gacctggtgg acctgttcgg ccgctacgac cggatgggcg tggaccagat
     2821 gctcacgtgg gtgcagttcg gcgggctgcc ccacgacagg atcatgcgga gcatggagct
     2881 catcgccgag cgcgtgctcc cgcagctcga accggccagg caggcgccgt gaccgccggc
     2941 gccgaggacg tcgccgtgct ccgccggacg gccatcgccg gcctcgggat cacagacgtc
     3001 gggaaggtct acgggcgctc accgggcgac ttcgccgccg acgccgcccg ccgggcggtg
     3061 gccgacgccg ggctggagat gggcgacgtc gacggcctcc tgctgagcaa cgggatctcg
     3121 ggcggcgtag acctgcggct ccagcgcgag ctcgggctgc acgacctccg cctcctggcg
     3181 cagatgaccg cggccggggc caccgccatc gcccaggtgc agtacgccgc cctcgcggtg
     3241 gcctccgggc tggcgtccac cgtcgtgtgc gtgcatgccg acgcaccgct gcggcccatg
     3301 gagtcctcgg gcgccaccta tcgctggggc gggggggccg ggcgaggccc cgcgggcgtg
     3361 gacgccctct ccttcgtggg cggcatgcga acggccaact cgggctacgc cctcgccgcc
     3421 cggcgccaca tggcccggta cggcacgacc agcgagcagc tcggggccct ggccgtcgcc
     3481 caaaggggct gggcggccaa gaacccgatc gcccggttcc gcacgccgat caccgtcgag
     3541 gaccaccagg cctcgagatg ggtcgtcgag ccgctgcacc tgctcgactg ctgcatggtc
     3601 tccaacgg
//
LOCUS       DASWGG010000386         6447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_550706, whole genome shotgun sequence.
ACCESSION   DASWGG010000386 DASWGG010000000
VERSION     DASWGG010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550706"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /gene="gatA"
                     /locus_tag="VF954_07075"
     CDS             <1..1255
                     /gene="gatA"
                     /locus_tag="VF954_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX9530881.1"
                     /translation="AAVDRGLDPGPLAGVPIALKDNLCTRGIPTTCSSQILAGWRPPY
                     DATVVARLRDAGAVVVGKTNLDEFAMGSSTENSAFGPTRNPHDHTRVPGGSSGGSAAA
                     VAAGFAPLALGSDTGGSIRQPAALCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFAST
                     VADAAALLEVIGGHDPADSTSIDRPPPRLLPTLGDGVGGLRVGVVRELTDGIDADVLD
                     RLHQAADALGAAGAQVGEASVPAAVYGLSAYYLIAPAEASSNLSRYDGVRYGLRVPGE
                     DVTAMYEATRAKGFGPEVKRRIMLGTYALSAGYYDAYYGQAQRVRTLIIRDFEAAYRD
                     FDVLLAPTAPTTAFRLGAKTDDPLAMYLSDVCTIPSNLAGDPAISVPFGSGADGLPVG
                     VQVLAPALGEEVLFRVAAVLEGAAP"
     gene            1252..2673
                     /gene="gatB"
                     /locus_tag="VF954_07080"
     CDS             1252..2673
                     /gene="gatB"
                     /locus_tag="VF954_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX9530882.1"
                     /translation="MTAGWETVVGLEVHCELRTGTKLFCSCRNSFGDEPNTNTCPVCL
                     GLPGSLPVLNQMAVEHAMRIGNALHCEVRPSIFHRKNYFYPDMPKDYQVSQYDEPINV
                     DGWLELPDGGRVGIERAHLEEDTGKTTHVGGGGRIHQAEHSLVDYNRAGVPLVEIVSR
                     PDLRSAEQARAYVAELRAILVATGASDGKMEEGSLRVDANVSVRPEGAAGYGTRCEIK
                     NMNSLRSLGRAIDHEVTRQIDLLTSGGRVVQETRHWDESDGRTHAMRSKEEAFDYRYF
                     PEPDLVPLDPDEAWRARVRSSLPVLPAARRQAVAAAVGLAPAEAATVVASDLDALVLA
                     AVAAGADPRLAFNRVANEVAADIDAAPGLDPSAFARLVTMEATGRLGATQAKAVLADL
                     LAKGGDPDAIARAKGFEAMEDDAVARAVDEAIGAHPEEWARLRDGDGKMTGFFVGLVM
                     KATAKKANGKVVTDLLRARAAGG"
     gene            2699..3487
                     /locus_tag="VF954_07085"
     CDS             2699..3487
                     /locus_tag="VF954_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530883.1"
                     /translation="MASAGLLVLGACGSAHKEPQAGSHQATTTAAPAATTSPGSATTT
                     PTAGPITAGPTTTTRAGSGPATTAKGAGGATATSAAPTAAPGQVGARPGRYVYDVSGA
                     VASRSTLTVDPPSGSDQRSTVTPQGSSTPQSDETFGYTASQILFVDLKVPQIGKEFQP
                     VPPVQASPVPAQVGQRWSWDMTSTDRTTTLHGDFAYTGTDTVTVGGQPVPVLVLSVGL
                     TFRQGATTTTNDETEWVSTQLNLQLKVHDDRSGTTLLLESTTPA"
     gene            complement(3524..3832)
                     /locus_tag="VF954_07090"
     CDS             complement(3524..3832)
                     /locus_tag="VF954_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530884.1"
                     /translation="MAPVKGGIAGVDTSGFVKIVWASPQSPGQLEDWYRASFDPSMRA
                     PFILGPGDSHADVLMTAFTVGGAVANVDLRQSPPSDPPPTSPAPPGPNTYVVVSVSRG
                     "
     gene            complement(4290..4994)
                     /locus_tag="VF954_07095"
     CDS             complement(4290..4994)
                     /locus_tag="VF954_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530885.1"
                     /translation="MSPVYRLLLRTLTSRARLVAMGFFALLPVIVAVAIRARPGADRG
                     QAGFALIDAYGLSLLAPVTALAFASAALGDLVDDRTLVYIWLRPLPTWQLAGAALGAT
                     LTIAVPLTVVPLVAAAAISGAGGSLVSATAASSAVAVVGYGSVFLTLGLRVQRALVWG
                     LAYVLIWEGAVGRTARGAARLSIGIHARSLLAHLDHHSLPKLSTSAPVAVFAPLAAAA
                     AAVALTVRWLATADVA"
     gene            complement(4991..5917)
                     /locus_tag="VF954_07100"
     CDS             complement(4991..5917)
                     /locus_tag="VF954_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9530886.1"
                     /translation="MTDATTIEVHDVTKWFGGLVALSDVSFRVGPGVTAVLGPNGAGK
                     STLLRLLCGLTSPSRGQVRVLGRNPRRDRRLLRSIGLVPQQEALFESLTGLDFVRLAA
                     DLNSLADPAGAASGALATVELDPADRRPVSAYSKGMRQRVKVAAAIVHDPEVVVLDEP
                     LEGLDPRQRLRMIGLFRSLGEAGRTVLVSSHVLDEVERFGSTVLVFAQGRLAAEGDFH
                     AIRDQMDDRPHRLRINTDRPRELGGALLAGGLVHGAWVEQEGLVVDTADVRRLRRAVA
                     PAARQAAARLLEMAPLDDDLESVFRYLVERRA"
     gene            complement(5914..>6447)
                     /locus_tag="VF954_07105"
     CDS             complement(5914..>6447)
                     /locus_tag="VF954_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530887.1"
                     /translation="SRVLSLYLAAPLTRGTYLLSKAAAVTTVTLSVTVGPPLLLLVGL
                     ALENDGPAGPLGMLSVLGRILLAGAILSAVYTALALAAASLTDRRAFAASGALLVLLG
                     GGAVVNVLVLGLHAPSLLLLLNLGRTPFELVQRIYGKPAFSNGITVIDDALACVAYVA
                     VGASVAIWRYLRLQVTR"
BASE COUNT          952 a         2405 c         2313 g          777 t
ORIGIN      
        1 cgccgccgtg gaccgcggtc tcgacccggg gccgctggcg ggggtgccga tcgccctgaa
       61 ggacaatctc tgcacgcgag gcatccccac cacgtgctcg tcgcagatcc tggcgggctg
      121 gcggccgccc tacgacgcca cggtcgtggc ccgcctccgg gacgcgggcg ccgtggtggt
      181 cggcaagacc aacctggacg agttcgccat gggctcgtcg acggagaact cagccttcgg
      241 cccgacgcgc aacccgcatg accacacccg ggtgccggga ggctcgagcg gcggcagcgc
      301 cgcggccgtg gccgccgggt tcgcgccgct cgccctcggg tccgacacgg gcggctcgat
      361 ccgccagccc gccgccctct gcggcgtggt gggcatgaag ccgacctacg gcttggtgtc
      421 gcggtacggc ctggtcgcct tcgccagctc gctcgaccag atcgggccgt tcgcgtcgac
      481 ggtggccgac gccgccgccc tgctcgaggt catcggcggc cacgaccccg cggacagcac
      541 gtcgatcgac cggcccccgc cccggctgct ccccaccctc ggcgacggcg tcggtggcct
      601 gcgggtcggc gtcgtgcgcg agctgaccga cggcatcgac gccgacgtgc tcgaccggct
      661 ccaccaggcg gccgacgccc tgggggcggc gggcgcccag gtcggggagg cctcggtgcc
      721 cgcggccgtc tacggcctgt cggcctacta cctcatcgcc ccggccgagg cgtcctccaa
      781 cctctcgcgc tacgacgggg tgcgttacgg gctgcgggtc ccgggcgagg acgtcacggc
      841 catgtacgag gccacccggg ccaaggggtt cggcccggag gtcaagcggc ggatcatgct
      901 cggtacctac gccctgtcgg ccggctacta cgacgcctac tacggccagg cccagcgggt
      961 gcggaccctg atcatccgcg acttcgaggc cgcctaccgc gacttcgacg tcctgttggc
     1021 cccgacggcg cctacgaccg ccttccgcct cggggccaag accgacgacc ccctggccat
     1081 gtacctgtcc gacgtgtgca ccatcccgtc caacctggcc ggcgacccgg ccatcagcgt
     1141 gcccttcggt tccggcgccg acggcctgcc ggtcggcgtc caggtcctcg cccccgccct
     1201 cggcgaggag gtcctgttcc gggtggcggc cgtgctcgaa ggggccgccc cgtgaccgcc
     1261 gggtgggaga cggtcgtggg cctcgaggtg cactgcgagc tgcggaccgg gaccaagctt
     1321 ttctgctcgt gccggaactc gttcggcgac gagcccaaca ccaacacctg cccggtgtgc
     1381 ctcggcctgc ccggctccct gcccgtcctc aaccagatgg cggtcgagca cgccatgcgc
     1441 atcggcaacg cgctgcactg cgaggtgcgg ccctcgatct tccaccgcaa gaactacttc
     1501 taccccgaca tgccgaagga ctaccaggtc agccagtacg acgagcccat caacgtcgac
     1561 ggctggctcg agctcccgga tggcgggcgg gtcggcatcg agcgggccca cctggaggag
     1621 gacaccggca agacgacgca tgtgggcggc ggcgggcgca tacaccaggc cgagcactcg
     1681 ctggtcgact acaaccgcgc cggcgtgccg ctggtcgaga tcgtcagccg gccggacctg
     1741 cgctcggccg agcaggcccg ggcctacgtg gccgagctgc gggccatcct ggtcgccacg
     1801 ggggcctccg acggcaagat ggaggagggc tcgctgcggg tggacgccaa cgtgtcggtg
     1861 aggcccgagg gcgccgccgg gtacgggacc cggtgcgaga tcaagaacat gaactcgttg
     1921 cgctctctag gccgcgccat cgaccacgag gtcacccgcc agatcgacct gctcaccagc
     1981 ggtgggaggg tcgtgcagga gacccgccac tgggacgagt ccgacgggcg gacccatgcc
     2041 atgcgctcaa aggaggaggc cttcgactac cgctacttcc ccgagcccga tctcgtcccg
     2101 ctggatccgg acgaggcctg gcgcgcccgg gtccggtcca gcctgcccgt gctgcccgcg
     2161 gcccgccgcc aggccgtcgc cgccgccgta gggctggcgc ccgccgaggc ggccacggtg
     2221 gtggcctccg acctcgacgc cctcgtgctg gccgccgtgg ccgcgggggc cgatccccgt
     2281 ctggccttca accgggtcgc caacgaggtg gctgccgaca tcgacgccgc gccgggcctc
     2341 gacccctccg cctttgccag gctggtcacg atggaggcga cgggccggct cggcgcgacc
     2401 caggccaagg ccgtcctggc cgatctgctg gccaagggcg gggacccgga cgccattgcc
     2461 cgcgccaagg gcttcgaggc catggaggac gacgcagtcg cccgggcggt cgacgaggcc
     2521 atcggcgcgc acccggagga gtgggcccgc cttcgcgacg gcgacggcaa gatgaccggg
     2581 ttcttcgtcg ggctggtcat gaaggccacc gccaagaagg ccaacggcaa ggtggtcacc
     2641 gacctcctgc gggcccgggc ggcaggaggc taggccgatg ctcgccaggc tggtggcgat
     2701 ggcgagcgcc gggctcctgg tcctgggcgc ctgcggctcc gcccacaagg agccgcaggc
     2761 cggctcccac caggccacga cgaccgcggc gccggcggcc accacgtccc cgggctcggc
     2821 cacgacgacg ccgacggctg ggcccatcac ggctgggccc accaccacga cccgcgccgg
     2881 atcgggcccg gcgaccaccg ccaagggcgc cggcggcgct acggcaacga gtgcggcgcc
     2941 gaccgccgcc ccgggccagg tcggggcccg gcccggccgc tacgtgtacg acgtcagcgg
     3001 cgccgtggcg tccaggtcga ccttgacggt cgacccgccg tccggctccg accagcgcag
     3061 caccgtgacc ccgcagggca gctcgacccc gcagtcggac gagacgttcg gctatacggc
     3121 cagccagatc ctgttcgtcg acctcaaggt cccccagatc ggaaaggagt tccagcccgt
     3181 cccgcccgtg caggcctcgc cggtgccggc ccaggtgggt cagaggtgga gctgggacat
     3241 gacctccacc gataggacca cgacgctgca cggggacttc gcctacacgg gaacggacac
     3301 cgtgacggtc ggcggccagc cggtcccggt cctggtgctg tcggtgggcc tgacgtttcg
     3361 ccagggcgcc acgacgacga ccaacgacga gaccgagtgg gtgtcgacac agctgaacct
     3421 ccagctcaag gtccacgacg accggtccgg caccacgctg ctgctggagt cgaccacccc
     3481 ggcctgagcc tcagcccgtc acctgaggtt tcgcaagcac ctgtcagccc cggctgacgc
     3541 tcaccaccac gtaggtgttg gggcccggcg gggccgggga ggtgggtggc gggtcgctcg
     3601 gcggagactg gcgcaggtcg acgttggcta cggctccgcc caccgtaaac gcggtcatca
     3661 gtacatccgc gtggctgtcg cccggcccga ggatgaaggg cgcccgcata gaagggtcga
     3721 agctcgctcg ataccagtcc tcgagctggc ccggggactg aggggaagcc cacacgatct
     3781 tcacaaaccc gctggtgtcc acaccggcga tcccgccctt gaccggcgcc aactccgatc
     3841 ggccaactga cgttgcacca gggagcggac cgagcacggg ctcacgcctg agtcgagcta
     3901 gcgcggccgt gtccgtgtgc gccgagcacc ctgcgagggc cgccattgcg gcaagagtga
     3961 ccacggcgca ccagacgcgg gcaggaacat tggcgcggac ggtcacccga gggttgccag
     4021 tcagaaccgg tcaacccggg ctgacgccga ccgccacgcc acaacgcgca ccgtcccgcg
     4081 aagccaagaa cgaccgtgct tggacatgac tctcccccga tccgctcggc caggccagag
     4141 tccggctccg ccgccttcga cgccgttgcg ccggtgctca tcgtgcggcc ggacgctcga
     4201 accggcaata gccccaacga gtcattcgcg cgtgcctaca aatggtgttt tgccggtgcg
     4261 cggccggcgg ccttgagacg cctctttgat caggccacgt cggccgtggc cagccaccgc
     4321 acggttaggg ccacggccgc ggccgcggcc gcgaggggcg cgaacaccgc gaccggggcc
     4381 gacgtcgaca gcttgggcag gctgtggtgg tccaggtggg cgagcagcga ccgggcgtgg
     4441 atgccgatcg acaggcgggc cgcgccgcgc gccgtgcggc cgaccgcgcc ctcccagatc
     4501 aacacgtacg ccagccccca cacgagggcg cgctggacgc gcagccccag ggtcaggaac
     4561 accgacccgt agcccaccac ggccactgcg ctcgacgccg cggtggccga gacgagcgac
     4621 ccgccggcgc cggagatcgc cgccgccgcg accaggggca ccacggtgag cggcacggcg
     4681 atcgtcaagg tcgcccccag ggcggcaccg gccagctgcc aggtgggcag cggccgcagc
     4741 catatgtaca cgagcgtccg gtcgtcgacg aggtcgccga gcgcggccga cgcgaacgcc
     4801 agcgcggtca ccggggcgag cagcgacagg ccgtaggcgt cgatcagcgc gaacccggcc
     4861 tgcccgcggt ccgcgccggg gcgggcccgg atggcgacgg ccacgatcac gggcagcagg
     4921 gcgaagaagc ccatggccac caggcgggcc cggctggtca gggtccgcag cagcagccgg
     4981 tagaccgggc tcacgcccgg cgctccacca ggtagcggaa cacgctctcc aggtcgtcgt
     5041 cgagcggggc catctccagc aggcgcgccg ccgcctgacg ggccgccggg gccaccgccc
     5101 gccggaggcg gcggacgtcg gctgtgtcga cgaccaggcc ctcctgctcg acccatgcgc
     5161 cgtggacgag gcccccggcc agcagcgccc cgcccagctc ccgaggccgg tcggtgttga
     5221 tgcgcagtcg gtgggggcgg tcgtccatct ggtcccggat ggcgtggaag tccccctcgg
     5281 cggccaggcg gccctgggcg aacacgagca cggtggagcc gaagcgctcg acctcgtcga
     5341 gcacgtggct ggacaccagc acggtgcgcc ccgcctcccc gagcgaacgg aagaggccga
     5401 tcatccgcag ccgctggcgg gggtcgaggc cctccagcgg ctcgtcgagg accaccacct
     5461 cggggtcgtg cacgatggcc gcggccacct tgacgcgctg gcgcatgccc ttggagtagg
     5521 ccgagacggg tcggcggtcg gcggggtcca gctcaacggt cgcgagggct ccggacgccg
     5581 ctcccgccgg gtcggccagg ctgttgaggt cggcggccag ccggacgaag tccaggccgg
     5641 tcagcgactc gaagagcgcc tcctgctggg gcaccagccc tatgctccgc aagagccggc
     5701 ggtcccggcg ggggttgcgg ccgagcaccc tcacctggcc acgcgacggc gacgtcaggc
     5761 cgcacagcag ccgcaggagc gtcgacttcc cggccccgtt gggcccgagc acggccgtca
     5821 cgcccgggcc cacccggaac gacacgtccg agagcgcgac gagcccgccg aaccacttgg
     5881 tcacgtcgtg gacctcgatg gtggtcgcgt ccgtcatcgg gtgacctgga gccgcaggta
     5941 gcgccagatg gccaccgatg cgccgacggc cacgtacgcg acgcaggcca gggcgtcgtc
     6001 gatcacggtt atgccgttgc tgaaggcggg cttgccgtag atgcgctgca ccagctcgaa
     6061 cggcgtccgg cccaggttga gcagcaacag gagggagggc gcgtggaggc cgagcaccag
     6121 gacgttgacc accgccccgc cgcccagcag cacgagcagg gcgccggatg ccgcgaaggc
     6181 ccttcggtcg gtgaggctgg cggcggccag cgccaacgcg gtgtagacag cggacaggat
     6241 ggcgccggcc agcaggatcc ggccgagcac ggacagcatg ccgagggggc cggcgggccc
     6301 gtcgttctcc agcgccaggc cgaccagcag cagcagcggc gggcccacgg tcacgctcaa
     6361 ggtgacggtc gtcacggcgg ccgccttgct cagcaggtag gtgccccggg tcagcggcgc
     6421 cgccaggtac aggctcagca cccgcga
//
LOCUS       DASWGG010000387         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_552991, whole genome shotgun sequence.
ACCESSION   DASWGG010000387 DASWGG010000000
VERSION     DASWGG010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552991"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VF954_07110"
     CDS             <1..252
                     /locus_tag="VF954_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX9530888.1"
                     /translation="ADAEAEGLDQIFTASGFEWREAGCSMCLAMNPDKLAPGERSAST
                     SNRNFEGRQGPGGRTHLVSPGVAAATAVAGHFIRPEELD"
     gene            252..848
                     /gene="leuD"
                     /locus_tag="VF954_07115"
     CDS             252..848
                     /gene="leuD"
                     /locus_tag="VF954_07115"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX9530889.1"
                     /translation="MQPIDAITGTAAPLDRSDVDTDQIIPAEWLKRVERTGFGEGLFG
                     AWRKDPDFVLNRPEHRGAAILVAGPMFGTGSSREHAVWALMDFGFRAVVSARFGDIFR
                     TNCTKAGLVPAQVDAAAGAALLKAVSDDPALEVTVDLVDRMVRAPAAGIETPFPMDDY
                     TRWRLMEGLDDIGLTLRHAAEIDAFEAGRPGWLPSMKG"
     gene            complement(980..>2412)
                     /locus_tag="VF954_07120"
     CDS             complement(980..>2412)
                     /locus_tag="VF954_07120"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX9530890.1"
                     /translation="VDYIEAGFPVASEGDFEAVSAIAGTVEGPAICGLSRTQLADVDR
                     CWAAVRHAARARIHVFISTSPVHMEAMLHMTPEQVVRETRSGVGRAREHTADVEFSPQ
                     DATRTPLDFMMEVLQAAVEAGATTLNIPDTVGYGIPWDFGALIQYVRKEVKGDYVLST
                     HCHNDLGMATANSLAGVAAGGARQVEVCVNGLGERAGNAALEEVVMAIKIRPDMFPGI
                     EIGVNTEEIARTSRLVARLTGYPIQYNKAVVGKNAFAHESGIHQHGVLADRTTYEIID
                     AASVGQTGRQIVLGKHSGRHAFSDTLEKMGLKVQGDALNAAFTRFKELADRKVEITDA
                     DLEAIVAEELGTTVDAGFSLEELEVKGGTVGIPQARVVLVRQSDKAEATAEGDGMIDA
                     ACNAIRQATGVEAKLTDFKVSSVTGGVDALGDVVIQLESDGVRVSGRGVSTDVVEASA
                     RAYLNAVNRIVRVRARNEVRRTEVGP"
BASE COUNT          331 a          870 c          839 g          372 t
ORIGIN      
        1 gccgacgccg aggccgaggg tctcgaccag atcttcaccg cgtcgggatt cgagtggcgc
       61 gaagccggct gttcgatgtg cctcgccatg aaccccgaca agctggcccc cggcgagcgc
      121 tccgcctcga cctccaaccg caacttcgag ggccgccagg gcccgggcgg ccgcacccac
      181 ctggtctcgc cgggggtggc cgcggccacg gccgtcgccg gccacttcat ccgtcccgag
      241 gagcttgact gatgcaaccc atcgacgcca tcaccggcac ggccgcgccg ctcgaccgca
      301 gcgacgtgga caccgaccag atcatcccgg cggagtggct caagcgggtg gagcgcaccg
      361 gcttcggcga ggggctgttc ggggcgtggc gcaaggaccc ggacttcgtg ctcaaccggc
      421 ccgagcaccg gggcgccgcg atcctggtcg ccgggccgat gttcgggacc ggctcgtcgc
      481 gcgagcacgc cgtatgggcc ctgatggact tcgggttccg ggccgtcgtg tcggcccggt
      541 tcggcgacat cttccggacc aactgcacca aggccgggct ggtgccggcg caggtcgacg
      601 cggcggcagg cgcggcgttg ctgaaggcgg tgagcgacga ccccgccctc gaggtcacgg
      661 tcgacctcgt ggaccgcatg gtgcgcgcgc ccgcggccgg catcgagacg ccgttcccca
      721 tggacgacta cacccggtgg aggctgatgg aggggctcga cgacatcggc ctgaccctcc
      781 gccacgccgc cgagatcgac gccttcgagg ccggccgccc aggatggcta ccgtcgatga
      841 agggctgaac tacctgtcac cccgggcgct cacccgcgac gagcgccagc tccagccact
      901 tccagtggca gtcgacgtcc tggaaccccg cgagccaggt agcgcgaggc gtgaccttcc
      961 tccgcccatt cggcgatggt cacgggccga cctcggtccg gcgcacctcg ttgcgggccc
     1021 ggacccgcac gatgcggttg accgcgttga ggtaggcgcg ggccgacgcc tccaccacgt
     1081 cggtcgacac gccccggccg gagaccctca cgccgtcgga ctcgagctgg atgacgacgt
     1141 ccccgagcgc gtccacgccg cccgtgaccg agctgacctt gaagtcggtc agcttggcct
     1201 cgacccccgt agcctggcgg atggcgttgc acgcggcgtc gatcatgccg tcgccttcgg
     1261 ccgtggcctc ggccttgtcg gactggcgga cgagcacgac ccgggcctgg gggatgccga
     1321 ccgtaccccc cttcacctcg agctcttcga gggagaagcc ggcatcgacg gtggtgccga
     1381 gctcctcggc cacgatggcc tccaggtccg catcggtgat ctccaccttg cggtcggcga
     1441 gctccttgaa ccgggtgaag gcagcgttca gggcgtcgcc ctggaccttg agccccatct
     1501 tctcgagggt gtcggagaag gcatggcggc ccgagtgctt gcccagcacg atctggcgac
     1561 cggtctggcc cacgctggcc gcgtcgatga tctcgtaggt ggtgcggtcg gccagcacgc
     1621 cgtgctggtg aatgcccgac tcgtgggcga aggcgttctt gccgacgacg gccttgttgt
     1681 actggatcgg gtatcccgtg agacgggcga cgagccggct ggtccgggcg atctcctcgg
     1741 tgttcacccc gatctcgatg ccggggaaca tgtcgggccg gatcttgatg gccatcacca
     1801 cctcttcgag ggccgcgttc ccggcccgct cccccagccc gttgacgcag acctcgacct
     1861 ggcgcgcccc gccggcggcg acgccggcca gcgagttggc ggtggccatg ccgaggtcgt
     1921 tgtggcagtg cgtcgagagc acgtagtcgc ccttcacctc cttgcgcacg tactggatga
     1981 gggcgccgaa gtcccacggg atcccgtagc ccaccgtgtc ggggatgttc agcgtggtcg
     2041 cgcccgcttc gaccgcggcc tggagcacct ccatcatgaa gtcgaggggc gtccgggtgg
     2101 cgtcctgggg gctgaactcc acatccgctg tgtgctcccg ggcccggccc acacccgaac
     2161 gggtctccct gacgacctgc tcgggcgtca tgtgcagcat ggcctccatg tgcaccgggc
     2221 tggtcgagat gaacacgtgg atgcgcgccc gggcggcgtg gcgtaccgct gcccagcacc
     2281 ggtcgacgtc ggccagctgg gtgcgcgaga gcccgcagat ggcgggtccc tcgaccgtgc
     2341 cggctatggc gctcacggcc tcgaagtcgc cctcgctggc gaccgggaac ccggcctcga
     2401 tgtagtcgac cc
//
LOCUS       DASWGG010000388         6821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_554545, whole genome shotgun sequence.
ACCESSION   DASWGG010000388 DASWGG010000000
VERSION     DASWGG010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554545"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VF954_07125"
     CDS             complement(<1..272)
                     /locus_tag="VF954_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530891.1"
                     /translation="MNVPRPGACRDFDAAADEVAVGGLDEPARSALLRHAATCSRCGP
                     LLQDLSEVADRLLLLAPETEPPTGFETRAVGRMVAGGETAPAMLASA"
     gene            complement(269..949)
                     /locus_tag="VF954_07130"
     CDS             complement(269..949)
                     /locus_tag="VF954_07130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9530892.1"
                     /translation="MMWPAARLDRLADDALLAAVGLGEPEAATVFVRRFQRRVYGLAL
                     VITCDRPLAEDVSQRAFERAWRHAGSYDPRRAGVTTWLLTITRNLAIDTVRLRRPEPI
                     DPWVVADLLPPAGDQDPADAAVAADQMGRLRLALGRLPIEQRRAVVLATIGGRTSTEI
                     GAIEGIPVPTAKHRLRTGLRKLRAAVAVTDGEDIAEESGPEGTAGEDTGAEGIAGEDT
                     GAEGTGAR"
     gene            1109..1957
                     /locus_tag="VF954_07135"
     CDS             1109..1957
                     /locus_tag="VF954_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530893.1"
                     /translation="MVARVQRRGRRRAVRSAVAVVVLAALAAGAGLLLHRSRAPATPA
                     CTASGPAGLFAVDLEQAANATTIASVARRDGLPDHAVTVALAAALQESKLRNVAFGDL
                     DSVGLFQQRPSQGWGPASELVVPRLATEAFFRALVKVPGWTALAVTEAAQRVQHSAAP
                     DAYAQWESEARTLAQGLTGETPATFACHFPMPARVGADEPLATALAADNGSGVVGATV
                     APGRGWVAAAWLVGHADAYRIAEVAYAGERWTAASGRWSRDASAGPEVTMLRAGVRTG
                     AEMAVP"
     gene            1988..5020
                     /locus_tag="VF954_07140"
     CDS             1988..5020
                     /locus_tag="VF954_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX9530894.1"
                     /translation="MARPAVTSTPAPGRGTTPAAPAAAPDADATALARRIAELGSGHR
                     ARAHGLSWSTERMLAWAMANPAFKTQLFRFVDVFPATSGDADVLRHIHEYFEGADAPR
                     LLDLGIEVADRLPLGGVLSAGVARRIISRMAHQFIVGATPGAAVEGLHRLWREGSAFT
                     VDLLGEKTVSEPEADAYTGRVHELLDALFRAAPGWAPDDHLERDDIGPLPRASISVKP
                     TALASSYAPLTRQAGLAQARGRLLPILRRARDQGASVWFDMEHYDVKDLTIELFRSVL
                     DEPELEDLHAGLVLQAYLRDSARDLEEVLGWASGRKGPPIGVRLVKGAYWDAETIHAG
                     AEGWPVPVWGDKAETDANYERCARLLHQHHGTVRAAFASHNLRSLAYAITYARDLGIP
                     DSGYEIQMLYGMAAPIHAAIRRLGFRLRVYCPVGELVPGMAYLVRRLLENTSNESFVR
                     ARLAEGRSLDALIAPPPAVALPDPPGLAVRADTDGRNPGPYAHEPAAEWRRRPAREAL
                     AAAVATAGRAGPLDVPAVVGGAPVRTAATIASVDPAQPERVVAVSAACGPAEADAAVA
                     AVTSGCDGWRRTPAAGRAAVLFRTAAWMRRRRASLAALEVFEAGKPWADADADVCEAI
                     DFCEYYGREILRLDSGGLVSSPPGEANRLTYQAKGVGVVIAPWNFPLAIPTGMVTAAL
                     VCGNGVVLKPAEQTPAVASQLVTALLESGLPPGALAFLPGWGEDVGAHLVRHPGVAFI
                     TFTGSKAVGLDIVAAAAVTTPGQRGVKRVIAEMGGKNAIVVDSDADLDQAVPAIVASA
                     FGYAGQKCSAASRVVVVEDVHDELVRRLAGAARQLRVGPPAEMGTEVGPLIEADAQAR
                     VARYVAGAPAQGQVVLARADVPTTGYFAGPTIVDRVDPAAPLATDEIFGPVLVVLGAP
                     SFGAAVELANRTDYALTAGVFSRSPDNIRLAAAELRAGNVYVNRGITGAVVGRQPFGG
                     YGLSGVGSKAGGPDYLHQFLDPRVVTENTVRQGFAPTG"
     gene            complement(5017..5583)
                     /locus_tag="VF954_07145"
     CDS             complement(5017..5583)
                     /locus_tag="VF954_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9530895.1"
                     /translation="MAPRRTRGPRFRLHRAAIQAERWIPRRLHWWLARRVFADVAAGT
                     CVLLFDDALRVLLVSSTYGAGWSTPGGFVGRGETAAAAARRELGEELGVHPALVEVAG
                     SVRADRPVVDVVFCGRLPTGGELRPDHLEIRSCQWFALDGELPRWDPEVPAALATLRR
                     CLGADGLAAVLTEEEQDRLRVLLVPDGR"
     gene            complement(5643..>6821)
                     /locus_tag="VF954_07150"
     CDS             complement(5643..>6821)
                     /locus_tag="VF954_07150"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX9530896.1"
                     /translation="DVDHFKWVNDSLGHDAGDRLLVELGRRLSLIVRPGDTVGRLGGD
                     EFGVLCEDLASDLVPAALAERIRAAVSVPFDVGGAELAPTISIGIAVAAAYGPDGPDA
                     VLRQADAAMYRAKERGRDRVESFDDGMRVRAMQRVRTETELRHGIERAEVEPWYQPYV
                     ELSSGVLVGAEALARWHHPERTLVYPAAFVAVAEETGLIVTLGEAILERAVMDASSWP
                     RGEIGPLTVSVNLSARQLLSPDLPGAVRRALAEAQLDPSRLCLEITESVLLSDSDQSS
                     QAVASLRDLGVRIAVDDFGTGYSSLTYLRRFSLDVLKIDKSFVAGIAQDPADRSIVAA
                     TIQLARDLGLVTVAEGVETEAQATDLAAMGCQYGQGFLWSPAVPATAFCHLLAARPAP
                     "
BASE COUNT          837 a         2543 c         2586 g          855 t
ORIGIN      
        1 gccgacgcca acatcgccgg cgcggtctcg ccccccgcta ccatgcggcc gacggcccgg
       61 gtctcgaatc cggtcggcgg ctcggtctcg ggcgcgagca gcagcagccg gtcggccacc
      121 tcggaaaggt cctggagcag cgggccgcac cggctgcacg tggcggcgtg ccgtagcagg
      181 gcggaacgcg ccggctcgtc gaggccaccg acggccacct cgtcggcggc ggcgtcgaag
      241 tcgcggcatg cgccggggcg gggcacgttc acctggcgcc ggtgccctcg gcgccagtgt
      301 cctcgccggc tatgccctcg gcgccagtgt cctcgccggc tgtgccctcg gggccagatt
      361 cctcggcgat gtcctcgccg tcggtgacgg caacggcggc ccgcagcttg cgcagcccgg
      421 tgcggagccg gtgcttcgcc gtggggacgg ggatgccttc gatggcgccg atctcggtgc
      481 tcgtgcgccc gccgatggtg gccagcacca ccgccctgcg ttgctcgatg ggcaggcggc
      541 cgagggcgag gcggagccgg cccatctggt cggccgcgac cgcggcgtcc gccgggtcct
      601 ggtccccggc gggcgggagc aggtcggcga ccacccaggg gtcgatgggc tcggggcgcc
      661 gcaggcgcac ggtgtcgatg gccaggttgc gggtgatcgt aagcagccaa gtggtcaccc
      721 ccgcccggcg ggggtcgtag gagccggcgt gtcgccaggc ccgctcgaag gcgcgctggg
      781 acacgtcctc ggccaggggc cggtcgcagg tgatgacgag ggccagcccg tagacccgcc
      841 gctggaagcg ccggacgaac accgtggccg cctcgggctc tccgagaccg acggcggcga
      901 gcagggcgtc gtcggccagg cggtcgagac gggcggcggg ccacatcatg tgagccacat
      961 catgtgagta ggtacgtgcg ggcggcccga tgggatcgcc gggccacgct cgccactccc
     1021 gaccgcgccg gcatggcccg tggataccac aggccggcct cccgggctgc cacccgccgg
     1081 tgccgggcgg ccgccaggat ggaggctcgt ggtagctcgg gtgcagcgca gaggccggcg
     1141 gcgggccgtg cggtcggcgg tcgccgtggt ggtcctcgcc gccctcgccg ccggcgccgg
     1201 gctgctgttg caccggtcga gggctccggc gacgccggcg tgcacggcgt cgggccccgc
     1261 cggcctgttc gccgtcgacc tggagcaggc cgcgaacgcc acgaccatcg ccagcgtggc
     1321 ccgccgcgac gggctgccgg accacgccgt gaccgtcgcc ctggccgccg cgctccagga
     1381 gtccaagctg cgcaacgtgg cgttcggcga tctcgactcc gtgggcctgt tccagcagcg
     1441 cccgtcccag ggatggggac cggcgtcgga gctggtcgtg ccccggctgg cgaccgaggc
     1501 cttcttccgg gccctggtga aggtgccggg ctggaccgcc ctggccgtga ccgaggcggc
     1561 ccagcgggtg caacacagcg cggccccgga cgcgtatgcc cagtgggaga gcgaggcgcg
     1621 gaccctcgca caggggctca ccggcgagac gcccgcgacc ttcgcctgcc acttccccat
     1681 gcccgcccgc gtcggcgccg acgagcccct ggccactgcc ctcgcggccg acaacggcag
     1741 cggagtcgtc ggcgccaccg tggccccggg gcggggttgg gtcgccgccg cctggttggt
     1801 gggacacgcg gacgcgtatc gcatcgccga ggtcgcctac gccggcgagc ggtggaccgc
     1861 ggcgagcggc cggtggagcc gggacgcgag cgcgggcccg gaagtcacca tgctgcgcgc
     1921 cggcgtccgg accggcgccg agatggctgt gccctgaccg ggagggcccg gggcgtatgg
     1981 tgaggatgtg gcccgcccgg ccgtgacctc gacgcccgca ccaggccggg ggacgacccc
     2041 ggcggcgccc gctgcggccc cggacgcgga cgccacggcg ctggcccggc ggatcgccga
     2101 gctcggctcc ggccaccggg cccgggccca cgggctgtcc tggtcgacag agcgaatgct
     2161 cgcctgggcc atggccaacc cggcgttcaa gacccagctg ttccggttcg tggacgtgtt
     2221 cccggcgacc agcggggacg ccgacgtcct gcgccacatc cacgagtact tcgagggcgc
     2281 cgacgctccc cggctgctcg atctgggcat cgaggtcgct gaccgcctcc cgctcggggg
     2341 tgtcctcagc gcgggggtgg cccggcggat catctcccgg atggcgcatc agttcatcgt
     2401 gggagccacc cctggggccg cggtcgaggg cctgcaccgg ctctggcgcg agggcagcgc
     2461 cttcacggtg gacctgctcg gggagaagac ggtcagcgag ccggaggccg acgcctacac
     2521 gggccgcgtg cacgagctgc tcgacgccct cttccgggcc gcgccgggct gggcgccgga
     2581 cgaccatctc gagcgcgacg acatcgggcc gctgccgcgg gcgagcatca gcgtcaagcc
     2641 gacggcgctc gcctcgagct acgccccgct gacccgccag gccggtttgg cccaggcccg
     2701 gggccggctg ctgccgatcc tgcggagggc ccgggaccag ggcgcatccg tgtggttcga
     2761 catggagcac tacgacgtga aggacctgac catcgagctg ttccgctcgg tgctcgacga
     2821 gcccgagctc gaggacctcc acgccgggct ggtgctccag gcctacctcc gggactcggc
     2881 ccgggacctg gaggaggtcc tgggctgggc gtcgggccgc aaaggtccac cgatcggcgt
     2941 tcgcctggtc aagggggctt actgggacgc cgagacgatc cacgccgggg ccgagggctg
     3001 gcccgtgccg gtctggggcg acaaggccga gacggacgcc aactacgagc gctgcgcccg
     3061 gctcctgcac cagcaccacg ggaccgtgcg ggccgccttc gcgtcccaca acctgcggtc
     3121 gctcgcctac gccatcacct acgcccgcga cctgggcatc ccggacagcg gctacgagat
     3181 ccagatgctg tacgggatgg cggcgccgat ccacgccgcc atccgccgcc tcgggttccg
     3241 cctccgggtc tactgcccgg tcggcgagct ggtccccggc atggcctacc tcgtgcgccg
     3301 actgctcgag aacacctcga acgagagctt cgtgcgcgcc cgcctcgccg aggggcggtc
     3361 cctcgacgcc ctgatcgccc ctcctcccgc ggtggccttg cccgatccgc cggggcttgc
     3421 ggtgcgcgcc gacaccgatg ggagaaatcc gggcccgtac gcccacgagc ccgcggcgga
     3481 atggcgccgg cggccggccc gggaggcctt ggcggcggcg gtcgccacgg ccggccgggc
     3541 cggtcccctc gacgtgcccg ccgtcgtcgg gggcgccccg gtgcgcacgg ccgccaccat
     3601 cgcgtccgtg gacccggccc agcccgaacg ggtggtcgcg gtgtcggccg cctgcggccc
     3661 cgccgaagct gacgcggcgg tggcggcggt gacgtccggg tgcgacggtt ggcgccggac
     3721 accggcggcc gggcgggccg ccgtcctgtt ccgcacggcg gcgtggatgc gccggcgccg
     3781 ggcatcgctg gcggccctgg aggtcttcga ggcgggcaag ccgtgggccg acgccgatgc
     3841 cgacgtctgc gaggccatcg acttctgcga gtactacggc cgggagatcc tgcgcctcga
     3901 ctccggtggc ctggtgtcgt cgccgccggg ggaggccaac cggctgacgt accaggccaa
     3961 gggcgtcggg gtggtcatcg ccccgtggaa cttcccgctc gccatcccca cgggcatggt
     4021 cacggccgcg ctggtgtgcg gcaacggggt ggtgctcaag ccggcggagc agactcccgc
     4081 ggtggcgtcg cagctcgtga ccgccctgct cgagtcgggc ctcccacccg gggcgctggc
     4141 cttcctgccc ggctggggcg aggacgtcgg cgcccacctc gtgcggcacc cgggcgtggc
     4201 tttcatcacc ttcacgggct ccaaggcggt cgggctcgac atcgtggcgg cggccgcggt
     4261 caccactccc ggccagcgcg gtgtgaagcg ggtcatcgcc gagatgggcg gcaagaacgc
     4321 catcgtcgtc gactccgacg ccgacctcga ccaggccgtt cccgccatcg tggcctcggc
     4381 cttcggctac gccgggcaga agtgctcggc ggcctcccgg gtcgtcgtcg tcgaggacgt
     4441 ccacgacgag ctcgtccgcc gcctcgccgg cgcggcccgc cagctccgcg tcgggccccc
     4501 cgccgagatg ggcaccgagg tcgggccgct catcgaggcc gatgcccagg cccgggtcgc
     4561 ccgctacgtg gcgggtgccc ccgctcaggg ccaggtcgtg ctcgcccggg ccgacgtgcc
     4621 cacgacgggc tatttcgccg ggccgaccat cgtggaccgg gtggacccgg ccgctcccct
     4681 cgccaccgac gagatcttcg ggccggtgct ggtggtgctc ggcgcgccgt cgttcggggc
     4741 tgctgtcgag ctggccaacc gcacggacta cgccctgacc gcgggcgtgt tctcccgctc
     4801 gcccgacaac atccggctgg ctgcggccga gctgcgagcc ggcaacgtct acgtcaaccg
     4861 gggcatcacg ggcgcggtcg tcggccgcca gcccttcggg ggctacggcc tgtccggcgt
     4921 ggggtccaag gccggcgggc ccgactacct gcaccagttc ctcgaccccc gggtcgtcac
     4981 cgagaacacg gtgcgccagg ggttcgcccc gaccggctag cggccgtcgg ggacgaggag
     5041 cacgcggagg cggtcctgct cctcctccgt gagcacggcg gcgagcccgt cggcgcccag
     5101 gcagcggcgc agggtggcca gggcggccgg cacctcgggg tcccaacggg gcagctcccc
     5161 gtcgagcgcg aaccactggc acgacctgat ctccaggtgg tcggggcgca gctcaccgcc
     5221 ggtcgggagc cggccgcaga acacgacgtc caccaccggc cggtcggcgc gcacggaccc
     5281 ggccacctcg accagggccg ggtgcacgcc cagctcctcg ccgagctcac ggcgggccgc
     5341 ggccgccgcg gtctcgcccc gcccgacgaa cccgcccggc gtcgaccagc cggcgccgta
     5401 ggtgctcgaa acgagcagca cacggagggc atcgtcgaac agcaggacgc acgtgcccgc
     5461 ggccacatcc gcgaagactc gccgggccag ccaccagtgg agccggcggg ggatccagcg
     5521 ctcggcctgg atggccgccc ggtgcagccg gaacctcggg ccccgggtgc ggcgcggtgc
     5581 catggcccgt ggttaccaca gccaccgccg gcggcgacgg atgccgtttg gcgggtcctc
     5641 ggctaagggg cggggcgggc ggcgaggagg tggcagaagg cggtggccgg gaccgcaggg
     5701 ctccacagga acccctgccc gtactggcag cccatggcgg ccaggtcggt ggcctgggcc
     5761 tcggtctcga cgccttcggc gaccgtgacg aggcccaggt cccgggccag ctggatggtg
     5821 gcggccacga tggaccggtc ggcggggtcc tgggcgatgc cggccacgaa gctcttgtcg
     5881 atcttcagca cgtcgagcga gaagcggcgg aggtaggtca ggctggagta gccggtgccg
     5941 aagtcgtcca ccgcgatccg cacgccgagg tcgcgcaggc tcgccaccgc ctgggagctc
     6001 tggtcggagt cggagagcag cacgctctcg gtgatctcca ggcacagccg cgacgggtcg
     6061 agctgggcct cggccagcgc ccggcgcacg gcgcccggca ggtccgggga cagcagctgg
     6121 cgggccgaca ggttgaccga cacggtgagg ggcccgatct cgccgcgggg ccagctcgac
     6181 gcgtccatga cggcccgttc gaggatggct tcgccgaggg tcacgatcag gccggtctcc
     6241 tcggccacgg ccacgaaggc ggcggggtag accagggtcc gctcggggtg gtgccaccgg
     6301 gccagcgcct cggcgccgac caggacgccg ctggacagct cgacgtaggg ctggtaccag
     6361 ggctcgacct cggcccgctc gatgccgtgg cgcagctcgg tctctgttcg gacccgctgc
     6421 atggcccgca cgcgcatgcc gtcgtcgaag gactcgaccc ggtcccggcc ccgctccttg
     6481 gcccggtaca tggcggcgtc ggcctggcgg agcacggcgt cagggccgtc cggcccgtac
     6541 gccgcggcga cggcgatgcc gatgctgatc gtcggcgcca gctctgcgcc cccgacgtcg
     6601 aaggggaccg agacggcggc gcggatgcgc tcggccaagg cggcgggtac cagatcggac
     6661 gccaggtcct cgcacaggac gccgaactca tcgcccccga ggcggcccac cgtgtccccc
     6721 gggcgcacga tcagggacaa ccggcgaccc agctcgacga gcaaccggtc ccccgcgtcg
     6781 tggcccaggc tgtcgttgac ccacttgaaa tggtcgacgt c
//
LOCUS       DASWGG010000389         4330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2160474, whole genome shotgun sequence.
ACCESSION   DASWGG010000389 DASWGG010000000
VERSION     DASWGG010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2160474"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VF954_07155"
     CDS             complement(<1..1003)
                     /locus_tag="VF954_07155"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX9530897.1"
                     /translation="MVDDVSRLPSSGSLSRDEVAGRTFRMVRRGFDPEEVRSFLDRVA
                     GELTRGHDREASLRRQTAEAEGRAAHPELDEATLAAALGQETARILRSAHEAAAGVRS
                     RAEDSVAAILRDAHEQAAAFRAESETVLATRTAEANEAAAAIRGGAEADTEAVLAGAR
                     QQADESMEAVRAECRSMIEEAQELRAKVLSDLARRRRVMHTQVEQLRAAREALLETFR
                     EAKRAIDVVHEGLQRAETDARLSAEAAARRVAAEPEASVEDLEAAIVQARDLEAIAPA
                     PAVLEERRISSVRILRPPAEEAAASSPPVAPAEPVPAPISPEQPEPLAEPEGRPGVEE
                     "
     gene            complement(1040..3628)
                     /locus_tag="VF954_07160"
     CDS             complement(1040..3628)
                     /locus_tag="VF954_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9530898.1"
                     /translation="MQFAAEFASFLAAAAATALIALRPDLLTASRRSRLVLFAGFVLL
                     TAVAFVHGSLLVADGSAPPLLALRGAGVVLVAAGLATWGRSPWWRPVLMAAMVVTAAG
                     VAVSVASGSSVAANALFGVGAVLEAAAISRASVPSVAARVAATAAVTLLLVILVLAVA
                     LSEVLSSTVRAEAFRRLDGRASTVANEVVRRSKDQVTAANVLRAEVQAIHAAMPADAG
                     GDTAPLQAQLEVLAQQFFTRDRLVWITSSGSVAASAAMGVDPVPLARYLAGSAIVRRA
                     LAGQNVPYGVLAFDGQLLVVAAYPNLVSGQAGSQVVGVAVAAEPVTAADLADAVSGDP
                     AVGLAIADRSGVLLAAGTPPPFADARAVAAQAIGPGRVPSTSVGTRESSSLLLDARPI
                     PGASGGPAGAVVAYTTRSSVTRTRDSLFRTLFLIALGGTLLALLVSALVGGQVGAGLR
                     ALTLAAARIRRGETGVRAGVHSDDEVGVLGTAFDSMSSSIEEQAEALREAAAAEARLR
                     NRLERVVAGMGEALIATDQGGQITELNPAAERLLGKHRDRVMGRPVDSVVKLRAEDGQ
                     DLALLLDPGESWSSPALLDTTGEPVPVAVSVAPLSGSDAETVGSVILVRDVRREREVE
                     RMKTEFLSRIGHELRTPLAGVMGYANLIAHRSLPAEQMKGFSEDILSQARRLQRVIEM
                     LEFFASSGAGRVMLRPEAMDVRALLRDAAATRSANGARVAVSVARGTPRILADSRWLA
                     TAVDELLDNAIKFSQGPALVTVRAEKAEEGRAVDVSVADQGKGMTPDELARAFGEFAQ
                     GDTSDTREFGGLGLGLPLVQRVAEGQGGAVTCSSEPGKGTRVTIRLPAAPPNERARKP
                     ARSARG"
     gene            complement(3702..>4330)
                     /locus_tag="VF954_07165"
     CDS             complement(3702..>4330)
                     /locus_tag="VF954_07165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX9530899.1"
                     /translation="IVAIDGRPTLLEADAAGILRQHRPGDVVALTVDPGGGRPTRVEQ
                     IALTGHQVSLPQTSCASAPGTTTVALLGVGLGTRNDKVDLPERITIDPQGIGGPSAGL
                     AFTLGIIAQLTSSDLSGGHKIAATGAISTDGTVGDVGGVPQKTVAVRRAGADLFIVPC
                     GELHDAQSKAGKHLRVVAVATLEQALGVLRADGGTMGNLPPAPATLLR"
BASE COUNT          575 a         1621 c         1614 g          520 t
ORIGIN      
        1 tctcctcgac tccaggccgg ccctcgggct cggccagggg ctccggctgc tctggggaaa
       61 tcggggccgg taccggttcg gccggcgcga ccggcggcga cgacgcggcg gcctcctcgg
      121 cgggaggccg caggatgcga accgacgaga tgcggcgctc ctccagcacc gccggggcgg
      181 gggcgatggc ctcgaggtcg cgggcctgca cgatggccgc ctcgaggtcc tcgacgctgg
      241 cctccggctc ggccgccacc ctcctggccg cggcctctgc cgacagccgc gcatcggtct
      301 cggcccgctg caaaccctcg tgcacgacgt cgatggcccg cttggcttcg cggaaggtct
      361 cgaggagggc ctcccgggcg gcgcgcagct gctcgacctg cgtgtgcatt acccggcgcc
      421 gtcgcgccag gtcgctgagc accttggccc gcagctcctg ggcctcctcg atcatggagc
      481 ggcattcggc gcggaccgcc tccatcgact cgtcggcctg ctgccgggcc ccggccagga
      541 cggcctcggt gtcggcctcg gcccccccgc ggatggcggc cgcagcctcg ttcgcctccg
      601 ctgtgcgggt ggcgaggacg gtctccgact cggcccggaa ggcggcggcc tgctcgtggg
      661 cgtcgcgcag gatggcggcc acgctgtcct cggcgcggga gcggacaccg gccgcggcct
      721 cgtgggcgct gcgcaggatg cgggccgtct cctggccgag cgcggccgca agggtggcct
      781 cgtccagctc gggatgggcc gcccgcccct cggcctcggc ggtctggcgg cgcagcgagg
      841 cctcacggtc gtggccgcgc gtcagctcgc ccgcgaccct atcgaggaag gaccggacct
      901 cctccggatc gaagccgcgc cgcaccattc ggaaggtccg cccggccacc tcgtcccggg
      961 acagcgaacc cgacgacggc agccgagaca cgtcgtccac catggcgatg aggataagac
     1021 ccgccgctcg ggcgcggggt catccccggg cgctgcgggc cggtttgcgg gctcgctcat
     1081 tcggcggggc cgcggggagt cggatcgtca cccgggttcc cttgcccggc tcggacgagc
     1141 acgtcaccgc gccgccctgg ccctcggcga cccgctgcac gagcggcagg cccagcccca
     1201 ggccgccgaa ctcgcgggtg tccgacgtgt cgccctgggc gaactcgccg aaggcgcggg
     1261 ccagctcgtc cggggtcatc cccttgccct ggtctgcgac cgagacgtcc acggcccggc
     1321 cttcctcggc cttctcggcc cggaccgtga ccagggccgg gccctgggag aacttgatgg
     1381 cgttgtcgag cagctcgtcc accgccgtgg ccagccagcg ggagtcggcc aggatgcggg
     1441 gcgttccccg tgccaccgag acggccaccc gggccccgtt ggccgagcgg gtcgcggcgg
     1501 cgtcgcggag cagggcgcgc acgtccatgg cctcggggcg cagcatcacc cggcccgcgc
     1561 cggacgaggc gaagaactcc agcatctcga tgacccgctg gagccggcga gcctgggaca
     1621 ggatgtcctc cgagaacccc ttcatctgct cggccggcag gctccggtgg gcgatgaggt
     1681 tggcgtagcc catgactccc gccagcgggg tgcgcagctc gtgtccgatc cgcgacagga
     1741 actcggtctt catccgctcg acctcccgct cccggcggac gtcgcgcacc aggatgacgg
     1801 acccgaccgt ctcggcgtcg gagcccgaaa gcggcgcgac tgagacggcc acgggcaccg
     1861 gctcgcccgt ggtgtccaga agggccgggc tcgaccacga ctcgccggga tcgagcagga
     1921 gcgccaggtc ctggccatcc tccgctcgaa gcttcacgac gctgtccacc ggccggccca
     1981 tcacccggtc tctgtgcttg ccgagcaggc gctcggcggc cggattcagc tcggtgatct
     2041 gccccccctg atcggttgcg atcagggcct cacccatgcc ggccacgacg cgctccagcc
     2101 ggttgcggag gcgggcctcg gccgcggccg cctcgcgcag cgcctcggcc tgctcctcga
     2161 tcgaggagga catggagtcg aacgccgtgc cgaggacgcc cacctcgtcg tcgctgtgga
     2221 ccccggcccg cacgccggtc tcgccccgcc ggatgcgcgc tgcggccagc gtcagggcgc
     2281 ggaggccggc gccgacctgc ccgccgacga gcgccgacac gagcagggcc agcagcgtcc
     2341 cgcccagggc gatgaggaaa agcgtgcgga acagggagtc gcgggtgcgg gtgaccgacg
     2401 accgcgtggt gtaggccacc accgcgcccg ccgggccgcc gctggcgccg gggatgggtc
     2461 gggcgtcgag gaggagggac gagctctcgc gcgtgcccac gctggtcgag gggacgcgcc
     2521 ccggaccgat ggcctgggcc gcgaccgccc tggcgtcggc gaacggcggg ggcgtccccg
     2581 ccgcgaggag cacgccgctg cgatcggcga tggccagccc gacggcgggg tcgccggaga
     2641 cagcgtcggc caggtcggcc gcggtgacgg gctcggccgc gaccgcgaca cctacgacct
     2701 ggctgccggc ctggccgctg accaggttcg ggtaggcggc gacgaccagg agctgcccat
     2761 cgaaggccag cacgccatag ggcacgttct ggccggccag ggcccgccgc acgatggccg
     2821 agccggccag gtagcgggcg agcgggacgg gatcgacgcc catcgccgcc gaggcggcaa
     2881 ccgaccccga cgacgtgatc cacaccagcc ggtctctcgt gaagaactgt tgggcgagga
     2941 cctcgagctg cgcctggagc ggcgccgtgt cccccccggc gtcagccggc atggcggcat
     3001 ggatggcctg gacctccgcc cgcagcacgt tggccgcggt cacctgatcc ttggaccggc
     3061 ggaccacctc gttggccacg gtggaggccc ggccgtccag gcggcggaag gcttcggccc
     3121 ggaccgtcga ggacaggacc tcggacaggg cgacggccag caccaggatg acgagcagca
     3181 acgtgaccgc cgccgtcgcc gccacccggg cagccacgga cgggacgctc gcccgcgaga
     3241 tcgccgccgc ctcgaggacg gcgccgactc cgaacagcgc gttggccgcg accgagctgc
     3301 ccgacgccac cgacacggcg acgccggcgg ccgtgaccac catggcggcc atgagcaccg
     3361 ggcgccacca tggcgagcgg ccccacgtcg ccaagccggc cgccaccagg accactccgg
     3421 cgccccggag cgcgagcagc ggcggggcgg aaccgtcggc gacgagcaac gagccatgga
     3481 cgaacgcgac cgcggtgagc agcacgaagc cggcgaacag gaccaggcgg gagcgccggg
     3541 acgccgtcag caggtcgggg cggagggcga tcagggccgt cgccgccgcc gccgccagga
     3601 agctggcgaa ctcagcggcg aactgcaact gagctagggc cgaggatcgc atgtccgtcc
     3661 tatcggagcc ggtcctctca gggtaaaacc ggcgccggac cctagcgcag cagcgtcgcc
     3721 ggagcgggcg gcaggttgcc catggtaccg ccgtcggccc tcagcacgcc gagcgcctgc
     3781 tcgagggtgg cgacggcgac gacccggagg tgcttgccgg ccttgctctg ggcgtcgtgg
     3841 agctcgccgc acggcacgat gaacaggtcc gccccggctc ggcgcacggc caccgtcttc
     3901 tgggggacgc cgcccacgtc gccgaccgtg ccgtccgtgc tgatcgcccc ggtggccgcg
     3961 atcttgtggc cgccgctcag gtcgctcgag gtcagctggg cgatgatgcc caaggtgaag
     4021 gccagcccgg ccgaggggcc gccgatgccc tgcggatcga tcgtgatcct ctccggcagg
     4081 tcgaccttgt cgttgcgcgt gcccaggccc acgccgagca gcgcgaccgt cgtggtgccg
     4141 ggggcgctgg cgcaggacgt ctgcgggagg ctgacctggt gaccggtcag cgcgatctgc
     4201 tcaaccctgg taggccggcc gccgcccgga tcgaccgtga gggccacgac gtcgcccggc
     4261 ctgtgctgac ggaggatgcc ggcggcgtca gcctcgagaa gggtgggacg cccgtcgatg
     4321 gccacgatga
//
LOCUS       DASWGG010000390         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_555900, whole genome shotgun sequence.
ACCESSION   DASWGG010000390 DASWGG010000000
VERSION     DASWGG010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555900"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1457
                     /locus_tag="VF954_07170"
     CDS             57..1457
                     /locus_tag="VF954_07170"
                     /inference="COORDINATES: protein motif:HMM:NF006915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinic semialdehyde
                     dehydrogenase"
                     /protein_id="HEX9530900.1"
                     /translation="MTAPTWEDTMAIATVNPATGETVKTFDALSIEELDARLGRAAEA
                     FQSYRLTTFAERAGWMRAAAGILDREADAIAVTMTTEMGKTLKSARAEAQKCAKACRF
                     YAEHAEAFLADEPADPAAVGAKQAFTRYLPLGPVLAVMPWNFPLWQVMRFAAPALMAG
                     NVGLLKHASNVPQTALFLEDVFSRAGFPDGAFQTLLIGSDLVERVLRDPRVVAATLTG
                     STPAGQSVASVAGHEIKKTVLELGGSDPFVVLPSADVDKAAEVGVVARCQNNGQSCIA
                     AKRFIVHEAVADEFERRFVERMAALRLGDPMDDSTDLGPLATAQGRKDVEELVADAVD
                     RGAWVLVGGQRPDGPGWFYPATVVTGITPEMRMYSEEVFGPVAQLYRVADADEALEVA
                     NATEFGLGSNVWTNDPAEQERFIRDMAAGAVFVNGMTTSYPELPFGGVGISGYGRELS
                     AHGIREFCNIKAIWVG"
BASE COUNT          241 a          552 c          573 g          243 t
ORIGIN      
        1 gcgcggggcg gggcggtggg agcgggaggg gtatacaggg cggtgagcag gaatctgtga
       61 cggcccccac ctgggaggac accatggcga tcgccaccgt gaacccggcg accggcgaga
      121 cggtcaagac gttcgacgca ctgtcgatcg aggagctcga cgccaggttg ggccgggcgg
      181 cggaggcctt ccagagctac cggctgacca cgttcgccga acgggccggg tggatgcggg
      241 cggcggccgg catcctcgac cgggaggccg acgccatcgc cgtcaccatg acgacggaga
      301 tgggcaagac cctgaaatcg gcccgggccg aggcccagaa gtgcgccaag gcctgccgct
      361 tctacgccga gcacgccgag gcgttcctgg ccgacgagcc ggccgacccg gccgccgtcg
      421 gagcgaaaca ggccttcacc cgctatctcc cgctggggcc cgtcctggcc gtcatgccct
      481 ggaacttccc gctgtggcag gtcatgcgct tcgccgcccc cgccctcatg gccgggaacg
      541 tggggctgct gaaacacgcc tccaacgtcc cccagacggc cctgttcctg gaggacgtct
      601 tcagccgggc cggcttcccc gacggcgcct tccagacgct gctcatcggc tccgacctcg
      661 tcgagcgggt gctgcgcgac ccccgggtcg tggccgccac cctcaccggg agcaccccgg
      721 ccggccagtc ggtggcgtcg gtggcgggcc acgagatcaa gaagaccgtg ctggagctcg
      781 gcggcagcga cccgttcgtc gtcctcccct cggccgatgt cgacaaggcg gccgaggtgg
      841 gagtggtggc ccgctgccag aacaacgggc agagctgcat cgccgccaag cgcttcatcg
      901 tccatgaggc ggtggccgac gagttcgaac gccgtttcgt cgagcggatg gccgccctgc
      961 ggctcggcga ccccatggac gactcgacgg acctcggccc cctggccacc gcccagggtc
     1021 gcaaggacgt cgaggagctc gtggccgacg ctgtcgaccg cggcgcctgg gtcctcgtcg
     1081 gggggcagcg gccggacggg ccgggctggt tctaccccgc caccgtcgtc accgggatca
     1141 ccccggagat gcgcatgtat tcggaggagg tgttcggccc ggtggcccag ctgtaccggg
     1201 tggccgacgc tgacgaggcc ctggaggtgg ccaacgccac cgagttcggt ctcggctcca
     1261 acgtgtggac gaacgacccc gccgagcagg agcgcttcat ccgcgacatg gccgccggcg
     1321 ccgtgttcgt gaacggtatg acgacctcct acccggagct cccgttcggg ggcgtcggga
     1381 tctcgggcta cggccgcgag ctctcggcgc acggcatccg ggagttctgc aacatcaagg
     1441 ccatctgggt cggttaattc tctcgttcac cggcccgcag gtgagcaggg cctgagaatc
     1501 attgagctgt gggccagttg tataacttga cgttgctttg cgttcacttc acaattcgtt
     1561 tcgtcagctg ggggctggga tcgggaagct ggggagccac cggtccgca
//
LOCUS       DASWGG010000391         2139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2443136, whole genome shotgun sequence.
ACCESSION   DASWGG010000391 DASWGG010000000
VERSION     DASWGG010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2443136"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VF954_07175"
     CDS             <1..403
                     /locus_tag="VF954_07175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9530901.1"
                     /translation="FGRGVFRWCHSLLDLEDPEAVWLPPDDERVPPWLAVFNGDVLVH
                     LDEDGRYGAGVGRKRHDANGSELSVATDERLRGRGLARRLVAQAARRVAEEGAVATYL
                     HATSNAASAKVAEAAGFPDTGWRVLGLPVAS"
     gene            463..1233
                     /locus_tag="VF954_07180"
     CDS             463..1233
                     /locus_tag="VF954_07180"
                     /inference="COORDINATES: protein motif:HMM:NF006100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9530902.1"
                     /translation="MSDESVVLTESRGAVLVITINRPDARNSMNRAAAEAMGAALDQL
                     DADPNLAVGVITGAGKGFSAGMDLKGFASGEFPVAGDRGFAGIAQRSAKKPLIAAIEG
                     FALAGGLEIALSCDVIVASREARLGIPEVGVGLFAGAGALLRLPRRVGLGQAALLALT
                     GKPISGEEGHRIGLVDRLVDAGSALDEALALAGTIAANAPLALAASKAVLVDGFTKPD
                     AEFWEWQRQFFNEVFTSKDAIEGATAFAEKRRPNWQGA"
     regulatory      1374..1457
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            1481..>2139
                     /locus_tag="VF954_07185"
     CDS             1481..>2139
                     /locus_tag="VF954_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530903.1"
                     /translation="MRVLATSLPSDLDECEAVAYAPPRRRLTTGSHAEFVGALVHEFR
                     GSLASLKGASELLARRRHELDEGVLGDLSTVIDRQAGRVAWLVRLLEAVDGDGSSRRV
                     EDVHGATVARQAAAGCDVALEVTTGADRDADVFAGDPERVRLGLEILFEALCPPGGAA
                     TARMPVAGYLTVVAPALDLDEQQRRFALRSAFRVLDMEGCALRVRTIGGETRAEVRLG
                     NR"
BASE COUNT          261 a          786 c          794 g          298 t
ORIGIN      
        1 tttcggccgg ggggtgttcc gctggtgcca ctcgctcctc gacctcgagg accccgaggc
       61 ggtctggctc ccacccgacg acgagcgggt gccgccgtgg ctggcggtgt tcaacggcga
      121 cgtcctcgtc cacctcgacg aggacgggcg ctacggcgcc ggggtcggcc gcaagcgcca
      181 cgacgccaac ggctccgagc tgtcggtggc caccgacgag cggctccggg gccggggcct
      241 ggcccgccgg ctggtggccc aggccgcccg tcgggtggcc gaggaggggg cggtggccac
      301 ctacctccac gccacgtcga acgccgcctc ggccaaggtg gcggaggcgg ccgggttccc
      361 cgacaccggc tggagggtcc tcggcctccc ggtggccagc tgagcgtccg gccgcccgcc
      421 ggggtaggtt gcatgcaaga actgactgtg aaggagagtc gaatgtccga cgagtccgtg
      481 gtcctgaccg agtcgcgggg tgcggtcctc gtgatcacca tcaaccggcc cgacgcccgc
      541 aactccatga accgggccgc cgccgaggcg atgggcgccg ccctcgacca gctcgacgcc
      601 gaccccaacc tcgccgtcgg cgtgatcacc ggcgccggaa aggggttcag cgccgggatg
      661 gacctgaagg gcttcgcctc cggcgagttc cccgtcgccg gtgaccgcgg cttcgccggg
      721 atcgcccagc ggtcggccaa gaagcccctc atcgccgcca tcgagggctt cgccctggcc
      781 ggcggcctcg agatcgccct gtcgtgcgac gtcatcgtcg cctcccggga ggccaggttg
      841 ggtatccccg aggtcggcgt cgggctcttc gccggcgccg gggcgctgct gcgcctgccc
      901 cgccgggtgg ggctgggcca ggcggcactg ctggcgctga ccggcaagcc gatcagcggc
      961 gaggagggcc accgcatcgg gctcgtcgac cggctggtcg atgccggctc ggccctcgac
     1021 gaggcgctgg ccctggccgg gaccatcgcc gccaacgccc cgctggccct ggccgcctcc
     1081 aaggccgtgc tggtggacgg cttcaccaag cccgacgctg agttctggga gtggcagcgg
     1141 cagttcttca acgaggtgtt cacgtcgaag gacgccatcg agggggcgac ggccttcgcc
     1201 gagaagcgcc ggcccaactg gcagggggcc tgaggcccgg cggcgggaac ccaatcgggc
     1261 aaagggcctt cggcttttcc gcagcgcagg tacacatgcc gtgaacgcgg ggttcggggc
     1321 gttgccgggc tccggcgaca cccctacggt cgttgcggaa cggattgaat ccgtcggtgt
     1381 gtggaggcga tgaccccccg tccgtatgtg gtcacgtgga cggtggcgag ccagtcgtga
     1441 acccaccctc gcgccgaccc cggttgaccg gaggtcgccc gtgcgcgtgc tggctacgtc
     1501 gttgccgtcg gacctggacg agtgcgaggc cgtggcctac gcccctcccc gacggcgtct
     1561 gaccaccggc tcgcacgccg agttcgtcgg cgcgctggtc cacgagttcc ggggttcgct
     1621 ggcctcactc aagggcgcga gcgagctgct ggcccggcgc cgccacgagc tcgacgaagg
     1681 cgtcctcggc gacctcagca ccgtgatcga ccgccaggcc ggccgcgtgg cctggctggt
     1741 gcgcctcctc gaggcggtcg acggcgacgg ctcgtcgcgc cgggtcgagg acgtccacgg
     1801 cgccacggtg gcccgccagg ccgccgccgg ctgcgacgtc gccctggagg tcaccaccgg
     1861 cgccgaccgg gacgccgacg tcttcgccgg cgaccccgag cgggtccgcc tcggcctcga
     1921 gatcctgttc gaggccctct gcccgccggg cggtgcggcg acggcccgca tgcccgtggc
     1981 cggctacctc acggtggtgg cgccggccct cgacctcgac gagcagcagc gccgcttcgc
     2041 cttgcggtcg gccttccggg tgctcgacat ggagggctgt gcgctgcggg tgcggaccat
     2101 cggtggcgag acccgcgccg aagtccgcct cgggaaccg
//
LOCUS       DASWGG010000392         7838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_558477, whole genome shotgun sequence.
ACCESSION   DASWGG010000392 DASWGG010000000
VERSION     DASWGG010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558477"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2404)
                     /locus_tag="VF954_07190"
     CDS             complement(<1..2404)
                     /locus_tag="VF954_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HEX9530904.1"
                     /translation="MGVPAASYGQVAVFTKVLVANRGEIACRILDTLRRLGIASVAVY
                     TDADAGSLHVRQADEAVRLGEGPVATSYLDVEKVLGAALATGADALHPGYGFLSESAA
                     FATACGAAGVTWIGPSAAHLRDFGDKHRARELARAAGLPLLAGTGVLGSVAEALAAAR
                     DVGYPVMLKATAGGGGIGMQHCDTPAALEAAYPSVGRLAKEHFGSAALYLERHVAAAR
                     HVEVQIFGARGEVGVLGLRDCSAQRRHQKVIEETPPPNLGAGLGRRLERWANRLGTSV
                     GYRSAGTVEFVVDVAGGEAAFLEVNTRLQVEHGITELITGVDLVEWMVLDADGSLSDL
                     GARVEEARARTGATAIEARVYAEDPAHDNRPGTGTLAHVAFPPGARVDTWVETGTEVT
                     PWYDPLLAKVLVSGPDRDAARNAMAAALADLRMDGVATNVGLLAQALADPRFVAGAYT
                     TSLLSDMTLPTPTIEVLEPGMQTTVQDWPGRPGYWAVGVPPSGPMDDRSFRLANELVG
                     NPPGAAGLEITRTGPTLRFETDATIALGGADVGATLDGEPVPRWSAIAVPAGGVLRSG
                     AVRGPGARAYLCVRGGIDVPEYLGSRSTFILGGFGGHAGRPLEPGDVLAVAPSSGLVA
                     VEPDDPLAKSSMVPALGKEWEIGVLDGPHGAPDFFTPEGIEELYAARWEVHHHSDRTG
                     VRLLGPVPRWARPDGGEAGLHPSNIHDTAYAVGTVDFTGDMPVVLGPDGPSLGGFVCP
                     ATVIAAERWKLGQLRAGDRVRFVPVTAVGAACAEAARRASIGLLRSLASPAASVGGTP
                     PVP"
     gene            2474..2968
                     /locus_tag="VF954_07195"
     CDS             2474..2968
                     /locus_tag="VF954_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9530905.1"
                     /translation="MGDSHRGEARPRFDDIDRDLVVALQKDGRASYARLAALVGMSQA
                     GVRARVQRLIDTAAVQIAAVADPFAFGFNVTAMVGITYTGDLDVLAAAVAELAQVHFV
                     VLTAGRYDSLAEVVCFDSDDLLALINDGIRAIPGVRDVEVITYLKLVKQWQPEYVRAV
                     RSAR"
     gene            3028..4227
                     /locus_tag="VF954_07200"
     CDS             3028..4227
                     /locus_tag="VF954_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9530906.1"
                     /translation="MAPADHHADHDMASLALARQLASTPSVSGAEADAVALVAGAMRA
                     LDYDEVQVDRSGNAIGTMHGGDGSRLLIDGHVDTIPLHSAERWSFDPFGGQVHDGRLY
                     GLGICDQKASIAAAVHGVARARREHRLHGPVAVVASVCEEAMEGQALAPVVAGFQPDI
                     VITTEPNDTRLGIGQRGRAKIEVTVTGRACHAGHSREGVNAVAALAALIGEAGRVEHP
                     AHPDLGRRDLTCIDIASWPYPSVSTVPGRAQARFDCRFLPGESPGSLLALLEECGWAA
                     WGDWPERPVLEVALVRAEFSTWTQARFEAEEFCAAWWTDEDSAPVVRAKSALTEAGLD
                     PTPTCYRFCTNGSLTAGDLGIPTLGFGVGVEAMAHQVDEHVTLASLAAGERGYTALAS
                     HLTAGGR"
     gene            4242..5048
                     /locus_tag="VF954_07205"
     CDS             4242..5048
                     /locus_tag="VF954_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX9530907.1"
                     /translation="MLPLTFGWEELPETVSIFGGDPGARLREPVPGVLVEVDGAWVLL
                     DTGFNDPLVRDPHLCRRFHGRNHDIHAELLPSDDDSLLAAFERVGVDVDDVAVVALSH
                     LHNDHAGGLRWFAERVPVHLQRTELAYGLSGHPEPERHGIFRVDFDDPVIDWRLADGE
                     TTIAPGLDAVPTYGHTPGHQSFVLRLTDRAAERYGVPGFVLAFDAADLQRNIDDEVAV
                     GGFIDCPPEQTVEAIRRLKAIAAQKGYRLVPGHDPVVWPAFTEELGVPGP"
     gene            5148..6530
                     /locus_tag="VF954_07210"
     CDS             5148..6530
                     /locus_tag="VF954_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9530908.1"
                     /translation="MAGIGSASVSLLVRGGTVVTAAGESRGDVLVGDDGRIVAVARDL
                     PAGGGAGARADRTIDAEGTIDAEGTIDADGTIDAGGMLVLPGMVDAHVHFDEPGRAEW
                     EGFATGSAAAVSGGVTTVIDMPIDCDPPTVTAEAVRAKVAAIERSSRVDVALWGGLTP
                     QSVKDLDGLFASGVVGLKAFACPSGWPEFPEVDEPVLAAGMAAAARAGVPVAVHCELA
                     AGRPSERSEVAAVRWAAKLATRSGAHLHVVHVSAAAAVDEARTVPGVSVETCAHYLLL
                     DEAAAAVVGPRAHCSPPIRDEVNRLALWERVRSGAVDVVASDHSPCPPSRRGGPAPWA
                     GIDGVGLTLPLLLSTGLLSRSRLVALTTAGARLAGLSRKGQLAPGFDADVVLVDPDAV
                     WTVGPATQWSRHRQSPFAGWTVKGRVETTLVRGRVAYTAANGPTDGAGGRALLRTTRT
                     ARTTGTARTA"
     gene            complement(6527..7525)
                     /locus_tag="VF954_07215"
     CDS             complement(6527..7525)
                     /locus_tag="VF954_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9530909.1"
                     /translation="MIRLLGKRCAAMVAVLLFLAAVVFTLQNMTPTDPVHSFLGANAS
                     KAAIKAESHKLGYDKPLPQQYLHYVGGMLHGDFQMSLRTRRPVATDLRRYLPATLELA
                     LFSLVLAAVLGGILGVVTAARFRGAGAFRLLTLAGASTPPFLLALVGILLLYHKLHWL
                     PATGRTSLASPPSGPTGLLTVDTLLHGNLGGFTDAFRHLVLPGVCVAVLPAVSIGRVL
                     RSSLVTTLRSDYVRTARAKGLRELVVLWRHAIRNSLGPALSMGGLQVGLMFAGVVVIE
                     QIFAWPGIGLYTVQSIPRSDFPAIAGVTIVLGAGYVVVNTLVDLLQAVADPRIAMG"
     gene            complement(7522..>7838)
                     /locus_tag="VF954_07220"
     CDS             complement(7522..>7838)
                     /locus_tag="VF954_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9530910.1"
                     /translation="EAARLAGVGRVRLAWRHLMPGAVPATMVTASLDVGNLVLTLAGL
                     SFLGLGAPAPAPELGAMAARGLTYLLQQWWVPVMPGLAVLALALLSNLAGDGLRDLLA
                     DR"
BASE COUNT         1091 a         2843 c         2895 g         1009 t
ORIGIN      
        1 gggggacggg gggagtcccc ccgaccgagg cggcgggaga cgccaaggaa cgcaacaggc
       61 cgatcgaggc ccgccgggcc gcttcggcgc aggcggcgcc cacggcggtg acgggcacga
      121 accggacccg gtcgccggcc cggagctggc cgagcttcca ccgctcggcg gcgatgaccg
      181 tggccgggca gacgaaccct cccaggctgg gcccgtcggg ccccagcacg accggcatgt
      241 cgccggtgaa gtcgaccgtg cccacggcat aggcggtgtc gtggatgttg gaggggtgca
      301 gcccggcctc accgccgtcg ggccgggccc agcggggcac gggtccgagc aggcgcacgc
      361 ccgtgcggtc cgaatgatgg tggacctccc aacgggccgc gtacagctct tcgatgccct
      421 cgggggtgaa gaagtcgggg gcgccgtggg gcccgtccag gacgccgatc tcccattcct
      481 tgccgagtgc cgggaccatg gacgacttgg ccaaggggtc gtcgggttcc accgcgacca
      541 gcccggacga gggtgcgacg gcgagcacgt cgcccggctc cagcggccgg ccggcgtggc
      601 ccccgaagcc gccgaggatg aacgtcgagc ggctccccag gtactccggt acgtctatgc
      661 caccccgcac gcagagatag gcccgggccc ccggcccgcg caccgcgccc gagcgcagca
      721 ccccgcccgc cggcaccgcg atggcggacc agcgcggcac cggctcacca tcgagagtgg
      781 cgccgacgtc agcgccaccg agggcgatgg tcgcgtcggt ctcgaaccga agcgtcgggc
      841 cggtacgggt gatctccaac ccggccgcac ccggagggtt gcctaccagc tcgttggcca
      901 gccggaagga ccggtcgtcc atcgggcccg acggagggac gccgaccgcc cagtacccgg
      961 gccggcccgg ccagtcctgc acggtggtct gcatcccggg ttcgagcacc tcgatcgtcg
     1021 gtgtcggcag cgtcatgtcg gagagcaacg aggtggtgta ggcgccggcg acgaaccgtg
     1081 ggtccgcgag cgcctgagcc agcaggccga cgttggtggc gacaccatcc atccggaggt
     1141 ccgcgagtgc cgccgccatg gcgttacgcg ccgcgtcgcg gtccgggccg gagacgagga
     1201 ccttggccag cagcgggtcg taccagggcg tcacctccgt ccctgtctcg acccaggtgt
     1261 cgacacgcgc cccgggtgga aaggcgacgt gggcgagggt gccggtcccc gggcggttgt
     1321 cgtgggccgg gtcctcggcg tagacgcggg cctcgatggc cgtcgcacca gtcctggccc
     1381 gagcctcctc gacccgggcc ccgaggtcgg agagcgaccc gtccgcatcc agcaccatcc
     1441 actcgacgag gtccacaccg gtgatcagct cggtgatgcc atgctcgacc tggagccggg
     1501 tgttgacctc gaggaaggcc gcctcgccgc ccgccacgtc gaccacgaac tcgaccgtgc
     1561 ccgccgagcg gtacccgacc gaggtcccca gccggttcgc ccaccgctcg agccggcggc
     1621 ccaggccggc gccgaggttg gggggaggcg tctcctcgat caccttctgg tgccggcgtt
     1681 gcgccgagca gtcccgcaga ccgaggacgc ccacctcccc acgggcgccg aagatctgca
     1741 cctcgacatg cctggccgcg gccacgtggc gctcgaggta caacgccgcc gagccgaaat
     1801 gctccttggc cagccgccct acggacgggt aggcggcttc gagggcggcg ggcgtgtcgc
     1861 agtgctgcat gccgatgccc ccgcccccgg cggtggcctt cagcatcacg ggatagccga
     1921 cgtcccgagc ggccgcgagc gcctcggcga ccgacccgag cacgccggtg cccgcgagca
     1981 gcgggaggcc ggccgcccgg gcgagctccc gcgcccggtg cttgtccccg aagtccctga
     2041 ggtgggcggc cgagggcccg atccaggtga cgccggccgc gccgcatgcc gtcgcgaagg
     2101 ccgcgctttc cgagaggaag ccgtagcccg ggtgcagcgc gtccgcgccg gtcgcgaggg
     2161 cggcgcccag caccttctcc acgtcgagat agctggtggc cacgggcccc tcgccgagcc
     2221 gcacggcctc gtcggcttgc cgcacgtgga gggacccggc atcggcgtcg gtgtacaccg
     2281 ccaccgacgc gatcccgagc cggcgcagcg tgtccaggat ccggcacgcg atctcgcccc
     2341 gattggcgac caacaccttg gtgaacacgg cgacctgacc gtaggaggcg gcggggacgc
     2401 ccatgtttct cactcatgaa caacgtgttt ccctctccgg gccgcgcctc gccgagcagt
     2461 acgctcagcc ggcgtgggcg acagccaccg cggcgaggcg cggccccgct tcgacgacat
     2521 cgaccgcgac ctggtggtgg cccttcagaa ggacggccgg gcctcgtacg cacggctggc
     2581 cgcgctggtg ggcatgtcgc aggcgggggt gcgcgcccgg gtccagcgcc tcatcgacac
     2641 cgccgcggtg cagatcgccg cggtggccga tcccttcgcc ttcgggttca acgtcaccgc
     2701 catggtcggc atcacctaca ccggcgacct ggacgtgctc gcggccgcag ttgccgagct
     2761 ggcccaggtc cacttcgtgg tgcttaccgc cgggcgctac gactccctgg ccgaggtcgt
     2821 gtgcttcgac agcgacgacc tgctcgccct gatcaacgac ggcatcaggg cgatccccgg
     2881 cgtgcgcgac gtcgaggtca tcacctacct caagctggtc aagcaatggc agcccgagta
     2941 cgtgcgcgcc gtcaggtcag caagatgaca ggccatgttc acgggaggga gacgcccgcg
     3001 ccggtccagc tcgctacgtt cgtccgcgtg gccccggccg accatcacgc cgaccatgac
     3061 atggcctccc tcgccttggc ccgccagctg gccagtaccc cgagcgtctc cggtgcggag
     3121 gccgacgccg tcgccctggt cgccggtgcc atgcgggccc tcgactacga cgaggtccag
     3181 gtcgaccggt cgggcaatgc cattgggacg atgcacggtg gcgacggctc ccgcctgctc
     3241 atcgacggcc acgtggacac catccccctg cactcggccg agcgctggtc tttcgatccc
     3301 ttcggcggcc aggtgcacga cgggcgcctg tacggcctcg ggatctgcga ccagaaggcg
     3361 tcgatcgcgg ccgcagtgca cggtgtcgcc cgggcccgcc gggagcacag gctgcacggc
     3421 ccggtggcgg tggtggccag cgtgtgcgaa gaggccatgg agggccaggc cctcgcgcca
     3481 gtcgtggccg gtttccagcc cgacatcgtg atcacgaccg agcccaacga cacccgcctc
     3541 gggatcggcc agcggggccg ggccaagatc gaggtgaccg tcaccggtcg ggcgtgccac
     3601 gccggccact cccgcgaggg ggtcaacgcg gtcgccgccc tggccgccct catcggcgag
     3661 gccggccggg tcgagcatcc cgcccacccc gacctcgggc gccgggacct cacttgcatc
     3721 gacatcgcct cgtggcccta cccgtcggtg tcgaccgtgc ccgggcgggc ccaggcccgg
     3781 ttcgactgcc gtttcctgcc gggggagtcg cccggctcgc tgctggccct gttggaggag
     3841 tgtggatggg ccgcgtgggg cgactggccc gagcggccag tcctcgaggt cgcgctggtg
     3901 cgggccgagt tctcgacgtg gacgcaggcc cggttcgagg ccgaggagtt ctgcgcggcg
     3961 tggtggaccg acgaggacag cgcccccgtg gtccgggcca agtcggcgct gaccgaggcc
     4021 gggctcgatc cgacgccgac gtgctaccgg ttctgcacca acggctcgct gacggcgggg
     4081 gacctgggca tcccgacgct cggcttcggc gtcggggtcg aggccatggc ccaccaggtg
     4141 gacgagcacg tgacgctggc gtctctggcc gccggcgagc ggggctacac cgcgctggcg
     4201 tcgcacctga cggcgggcgg gcggtgaacg gcgtcgagcg ggtgctcccc ctgaccttcg
     4261 ggtgggagga gctgcccgag acggtctcga tcttcggagg agatccgggc gcgaggctgc
     4321 gcgagcccgt gccgggtgtc ctggtcgagg tcgacggcgc gtgggtgctg ctcgacaccg
     4381 gcttcaacga cccactcgtg cgcgaccccc acctgtgccg ccgcttccac ggccgcaatc
     4441 acgacatcca cgccgagctg ctgccatcag acgacgactc cctcctggcc gccttcgagc
     4501 gggtcggcgt ggatgtcgat gacgtcgccg tcgtcgccct cagccatctg cacaacgacc
     4561 acgccggtgg cctgcgctgg ttcgcggagc gcgtccccgt ccatctccaa cgcaccgagc
     4621 tcgcctatgg gctgtccggc catcccgagc ccgagcgcca cggcatcttc cgggtggact
     4681 tcgacgaccc ggtcatcgac tggcgactgg ccgatggcga gacgacgatc gcccccggcc
     4741 tggacgcggt gcccacctac ggccacaccc ctggccacca gagcttcgtg cttcgcctca
     4801 ccgaccgcgc cgccgagcgg tacggcgtcc ctggcttcgt cctggccttt gacgctgccg
     4861 acctccagcg aaacatcgac gacgaggtgg cggtcggggg cttcatcgac tgcccacccg
     4921 agcaaacggt cgaggccatc cgccggctga aggcgatcgc cgcccagaag ggctaccggc
     4981 tcgtgccggg ccacgacccg gtggtgtggc cggccttcac cgaggagctc ggcgtacccg
     5041 gaccgtgacc ggatgacggc ccgccggcct tctccgccgg attctgttga cgccaatcgc
     5101 gccataggcg cgctacgcgt caacagaatg ctttcacaag cgagttggtg gccgggatcg
     5161 gaagcgcgtc cgtgagcctg ctcgtgcgcg gcggcaccgt cgtcaccgcg gccggggaga
     5221 gcaggggaga cgtcctcgtc ggcgacgacg ggcgcatcgt ggccgtcgcc cgcgatctcc
     5281 ctgccggcgg cggcgccggc gcccgcgccg accggacgat cgacgcggaa gggacgatcg
     5341 acgccgaagg gacgatcgac gccgacggga cgatcgacgc cggggggatg ctcgtgctgc
     5401 ccgggatggt cgatgcccac gtgcatttcg acgagccggg ccgggccgag tgggagggct
     5461 tcgccaccgg tagcgcggcg gccgtctcgg gcggggtcac gaccgtgatc gacatgccga
     5521 tcgactgcga cccgccgacg gtgaccgccg aggccgtgcg ggccaaggtg gcggcgatcg
     5581 agcggtcgag ccgggtggac gtggccctgt ggggcgggct gacgccgcag tcggtcaagg
     5641 acctcgacgg cctgttcgca tccggcgtgg tcgggctcaa ggcgttcgcc tgcccgagtg
     5701 gctggcccga gttccccgag gtcgacgagc ccgtcctggc ggccgggatg gccgccgccg
     5761 ccagggccgg ggtgcccgtc gcggtgcatt gcgagctcgc cgccggccgg ccgtcggaac
     5821 gctcggaggt ggcggccgtg cggtgggccg cgaagctggc aacccgtagc ggcgcccacc
     5881 tgcacgtggt ccacgtgtcg gctgccgctg ccgtggacga ggccaggacc gtccccggcg
     5941 tctcggtcga gacctgcgcg cactacctgc tgctcgacga ggccgccgca gccgtcgtcg
     6001 ggccgagggc ccactgctcg ccgccgatcc gcgacgaggt caaccggctc gccctgtggg
     6061 agcgggtgcg gtccggcgcc gtcgacgtcg tggcctcgga ccactcaccg tgcccgccgt
     6121 cgcggcgggg cggccccgcc ccgtgggccg gcatcgacgg cgtaggtctc accctgccgt
     6181 tgctcctgtc gaccgggctg ctcagccgtt ctcgcctggt cgcgctcacc acggcggggg
     6241 cccgcctggc cggcctcagc cggaagggac agctggcacc cggtttcgac gccgacgtgg
     6301 tgcttgtcga ccccgacgcc gtctggacgg tcgggccggc cacgcagtgg tcgcggcacc
     6361 ggcagtcacc cttcgccggc tggaccgtga agggacgggt cgagaccacc ctcgtgcggg
     6421 gccgggtcgc ctacaccgca gcgaacggcc cgaccgacgg ggcgggcggc cgggccctcc
     6481 tccggactac acggacggca cggaccacag ggacggcacg gacggcctag cccatggcga
     6541 tgcgaggatc ggccacggcc tggaggaggt cgacgagagt gtttacgacc acgtagcccg
     6601 cgccgagcac gatcgtgacc ccggcgatgg ccgggaagtc gctccggggg atggattgca
     6661 cggtgtagag gccgatcccc ggccaggcga agatctgctc gatgacgacc acgcccgcga
     6721 acatcaggcc gacctggagc ccgcccatcg acagcgccgg gcccaggctg ttgcggatgg
     6781 cgtggcgcca cagcacgacc agctcgcgca gtcctttggc ccgggcggta cgcacgtagt
     6841 cggaccgcag cgtcgtcacc aggctcgagc gcaggacccg gccgatggac acggccggca
     6901 ggacggcgac gcagacgccc gggaggacga ggtggcggaa ggcgtcggtg aagccgccga
     6961 ggttgccgtg caggagggtg tcgacggtga gcaggccggt cggcccgctc ggcgggctgg
     7021 cgaggctggt gcggccagtc gcgggcagcc agtggagctt gtggtacagc aggaggatcc
     7081 ccaccagcgc cagcaggaac ggcggggtcg acgccccggc cagggtgagg agccggaagg
     7141 ccccggcgcc ccggaagcgc gccgccgtga ccacgcccag gatgccgccg agcacggccg
     7201 ccagcaccag gctgaacagc gccagctcga gcgtggccgg caggtacctg cgcaggtcgg
     7261 tggcgacggg ccggcgggtc cgcagcgaca tctggaagtc cccgtgcagc atgcccccga
     7321 cgtagtggag gtactgctgg ggcagcggct tgtcgtagcc gagcttgtgg ctctcggcct
     7381 tgatggcggc cttggaggcg ttggccccca ggaacgagtg gaccgggtcg gtcggcgtca
     7441 tgttctggag ggtgaagacc accgcggcca ggaacaacag caccgcgacc atggccgcgc
     7501 accgcttgcc gaggagccga atcaccggtc cgccaacagg tcgcgcaggc cgtcgcccgc
     7561 caggttggag agcagggcga gcgcgagcac ggcgaggccg ggcatgaccg gcacccacca
     7621 ctgctggagc aggtaggtca agccgcgggc ggccatggcc ccgagctcgg gggcgggcgc
     7681 gggcgccccg agtccgagga aagacagccc ggcgagggtc aggaccaggt tgccgacgtc
     7741 gaggctggcc gtgaccatgg tggccggcac ggcgccgggc atgaggtgcc gccaggccag
     7801 ccggacgcgg ccgacgccgg ccagccgggc cgcctcca
//
LOCUS       DASWGG010000393         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_558816, whole genome shotgun sequence.
ACCESSION   DASWGG010000393 DASWGG010000000
VERSION     DASWGG010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558816"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VF954_07225"
     CDS             complement(<1..486)
                     /locus_tag="VF954_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530911.1"
                     /translation="MDIAHDATFLAMVTADVAGEATEEDRAAIAADPGSWAAVLWRML
                     DETDEALDRSEALAPDLRRQVQPDLDGEWERIARRLRDVTGADLLTAVADERPAAAPA
                     HPVEPGALGLHATWSGRRVVVWSGGPGVTPLAGEDLGAALAEASGGSVEWEPHPAVPL
                     PD"
     gene            715..2064
                     /locus_tag="VF954_07230"
     CDS             715..2064
                     /locus_tag="VF954_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX9530912.1"
                     /translation="MIPALRPAVAIRVLAAALLAGAVLAEARGSAGAGSAIQPNNRPT
                     PVAGQTNGQMDASQLVNVAPHCAAYRAAGPSLGLVLAEARQQGVPLATSQCYRPLADQ
                     VAVQQSWTSAGNSACAAPVVTTPSGQKGGTSMHGWGKAADFSDSGHGYAFGSSLDRWL
                     RANANQAGWNHPGWADPGGSACPEVWHWEWLGDGGTLGGLPIRADVVGVVPSADDSGY
                     LSVGGLGGVVARGDAVDRGSAAGVPINWLIVSAAGTPDRGGYWEVGSDGGIFSFGDAA
                     FLGSTGSIRLNQPIVGMAPTSSGNGYWLVASDGGVFSFGDAGFFGSTGGLRLNRPIVG
                     MAPTPSGQGYWLVASDGGIFSFGDAVFRGSTGAIALNRPVVAMVPTAPGGGYWLVASD
                     GGVFNFGDAAFLGSAGAQPPALPVTAAARTRHGGGYWLAVADGTILPFGDAKGFGAA"
     gene            complement(2230..>2604)
                     /locus_tag="VF954_07235"
     CDS             complement(2230..>2604)
                     /locus_tag="VF954_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX9530913.1"
                     /translation="ADEGPGLDEEAAGRVFERFYRVDKARTRDSGGVGLGLAIVAAIA
                     QAHGGRAMVRSQPGQGATFRVELPAGGPGPPGPAQPGGSSAAAPAPPAAAWGAPPATA
                     SPATASPPATASPWAPPAGASG"
BASE COUNT          300 a          976 c          995 g          333 t
ORIGIN      
        1 gtcggggagg gggacggcgg ggtgcggctc ccattcgacc gagccgccgc tcgcctcggc
       61 cagggcggcg ccgaggtcct cgccggcgag gggggtcacg ccgggcccgc ccgaccagac
      121 cacgacccgg cgcccgctcc aggtggcgtg gaggccgagg gcgcccggct cgacggggtg
      181 cgccggggcg gcggccggcc gctcgtcggc caccgccgtc aacaggtctg cgccggtgac
      241 gtcccgcagg cgccgggcga tccgctccca ctcgccgtcc aggtctggct ggacctggcg
      301 gcgaaggtcg ggcgcgagtg cctccgagcg gtccaaggcc tcgtcggtct cgtccagcat
      361 cctccacagg acggccgccc acgacccggg gtcggccgcg atcgcggccc ggtcctcttc
      421 ggtggcctcg ccggcgacgt cggcggtgac catggctaga aatgtcgcgt cgtgcgctat
      481 gtccattgat ccttcgacct cagcccggag catgcgctcc gtgcagtcgg gaccgggtcc
      541 ccggggtgct caaccgtacg gccggtccac cggaccgacc agctaggggc cgtcgagctg
      601 accttcagga tagcccgtgg aacgcagcaa cgccgacttg ggcccggtgc attcccgggc
      661 gcacggggaa ggcgccggag atggtggttc aggagatggt ggttcaatgg ggccgtgatc
      721 cctgcgctgc ggccggcagt ggccatccgg gtgctcgccg ccgcgctgct cgcgggggcg
      781 gtgctcgccg aggcccgcgg gagcgccggc gcagggagcg ccatccagcc caacaaccgg
      841 cccacgcccg tggccggcca gaccaacggc cagatggacg cctcccagct ggtcaacgtg
      901 gcgccccact gcgcggccta ccgggcggcc ggccccagtc tcggcctcgt cctcgccgag
      961 gcccgccagc agggcgtgcc cctcgccacc agccagtgct accgacccct cgccgaccag
     1021 gtggcggtcc agcagtcgtg gacgtcggcc ggcaacagcg cctgcgcggc gccggtcgtg
     1081 accacgccga gcggccagaa gggcggcacc tccatgcacg ggtggggcaa ggccgccgac
     1141 ttctccgaca gcggccacgg ctatgccttc gggtcgtccc tggaccgctg gctgcgggcc
     1201 aacgccaacc aggcgggctg gaaccatccc gggtgggcgg acccgggcgg cagcgcctgt
     1261 cccgaggtct ggcactggga atggctgggt gacggcggca cgctcggcgg cctgcccatc
     1321 cgggccgatg tggtgggcgt ggtcccgagc gcggacgaca gcggctacct cagcgtgggc
     1381 ggcctcggcg gggtcgtggc ccggggcgac gcggtcgacc ggggatcggc cgccggggtg
     1441 ccgatcaact ggttgatcgt gagcgcggcc ggcacccccg accgcggcgg gtactgggag
     1501 gtgggctcgg acggcgggat cttcagcttc ggcgacgccg cgttcctcgg atcgaccggt
     1561 tcgatccggc tcaaccagcc gatcgtcggg atggcgccga cgtcttccgg caacggctac
     1621 tggctggtgg cgtcggacgg gggggtcttc agcttcggcg acgccggttt cttcggctcc
     1681 accggcgggc tgcggctcaa ccgccccatc gtcgggatgg cgcccacccc gtctggccag
     1741 ggctactggc tggtggcgtc ggacgggggg atcttcagct tcggcgacgc cgtgttccgg
     1801 ggctcgaccg gcgccatcgc cttgaaccga cccgtcgtcg ccatggtgcc cacggcgccg
     1861 ggcggggggt actggctcgt ggcctccgac ggcggcgtgt tcaacttcgg cgacgccgcc
     1921 ttcctcggct ccgccggcgc ccagccgccc gccctgccgg tcactgcggc cgcccgcacg
     1981 cgccacggag ggggctactg gctcgccgtc gccgacggga cgatcctgcc cttcggtgac
     2041 gccaaggggt tcggggcggc ctgaccgccg ggttgccgca ttcttggcac cgaaaagcgt
     2101 ggaaaacacc ccctttcggt gccaagaacc gagttgactg gcccggccgg ggccccaacc
     2161 ccggcctcct ccgcacggcg cggccccaac cgcgccggcg gcggtgcaga gttccgtggc
     2221 cgcccgccct cagccggagg cgccggccgg gggagcccag ggggacgccg tggccggcgg
     2281 ggacgccgtg gccggggacg ccgtggccgg cggtgccccc caggcggccg ccggaggcgc
     2341 tggcgccgcg gccgaggagc cacccggctg agccgggccg ggcgggccgg gcccgccggc
     2401 gggcagctcg acccggaacg tcgctccctg gccaggctgg ctgcggacca tggcccggcc
     2461 accgtgggcc tgggcgatgg cggccacgat ggccaggccg aggccgacgc cgccactgtc
     2521 tcgggtgcgc gccttgtcca cccggtagaa ccgctcgaac acccggcccg ccgcctcctc
     2581 gtcgaggccg ggtccctcgt cggc
//
LOCUS       DASWGG010000394         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2164970, whole genome shotgun sequence.
ACCESSION   DASWGG010000394 DASWGG010000000
VERSION     DASWGG010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164970"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VF954_07240"
     CDS             complement(<1..215)
                     /locus_tag="VF954_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530914.1"
                     /translation="MSSDSPTPPVARRVPHVHEAHGDRRPDDYHWLRDRDDPEVTAYL
                     EAENAYADSVMEPLRPLQEKLYAEIVSR"
     gene            314..1501
                     /gene="ftsY"
                     /locus_tag="VF954_07245"
     CDS             314..1501
                     /gene="ftsY"
                     /locus_tag="VF954_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX9530915.1"
                     /translation="MELVLVLGIIVVLALAATGVGLAASKRSQRRLDAAERAVVDKTP
                     TQARPQPEPIVEPAGVEIVLPPGDEVVVEPEPEPLAPEPVVGEPAAPPPPAVRPRLRD
                     RLGKARALLAGYFGDLRGRSVIDEEVWNDLEEALIRADVGVMATERILDDLRRRVKEA
                     SVTSPSELLDLLKADLVDTLRTSDRALTFEPGQPNVWMFVGVNGVGKTTSIGKIAQRE
                     AAAGRRVVMAAADTFRAAAAEQLQLWSQRVGADLVRGQDGADPGSVVFDAMSAAANRG
                     ADLVLVDTAGRLHTKVNLMEELKKLRRIVDRTPGALKEVLLVIDASTGQNGLNQAKIF
                     GEAVDVTGVILTKLDGTAKGGIVLAVQTELGIPVKLVGVGESAEDLIEFDPEEFVDAL
                     FAA"
BASE COUNT          231 a          583 c          569 g          230 t
ORIGIN      
        1 cggctcacga tctcggcgta gagcttctcc tgcagcggcc gcagcggctc catgaccgaa
       61 tcggcgtagg cgttttccgc ctcgaggtac gccgtcacct cgggatcgtc ccggtcccgc
      121 agccagtggt agtcgtccgg ccgccggtcc ccatgcgcct cgtgcacatg cggaacccgg
      181 cgggccaccg gcggggtggg ggagtcgcta ctcatcgtcg ggacactacc tttagtcccc
      241 cgacgccacc cccgctgcca ccccggcggg gggcaggctg ggtccgcccc gacgaccggt
      301 aggatcgccc gccatggagc tggtgctcgt tctcgggatc atcgtcgtgc tggccctcgc
      361 cgctaccggt gtgggcctgg ccgcctccaa gcgctcgcaa cgccgcttgg acgccgccga
      421 gcgggcggtg gtggacaaga cccccaccca ggccaggccc cagcccgaac ccatcgtgga
      481 accggccggt gtggagatcg tcctcccgcc gggcgacgag gtcgtcgtcg agccggagcc
      541 cgagcccctg gcaccggagc cggtcgtcgg ggagccggcc gccccgccgc ccccggccgt
      601 ccgcccccgg ctgcgcgacc ggctgggcaa ggcccgggcg ctgctggccg gctatttcgg
      661 tgacctccgg ggccgcagcg tcatcgacga ggaggtgtgg aacgacctgg aggaggcgct
      721 catccgggcc gacgtcggtg tcatggccac cgagcggatc ctcgacgacc tccgccgccg
      781 ggtgaaggag gcgtcggtca ccagcccctc cgagcttctc gacctgctca aggccgacct
      841 ggtcgacacg ctgcgcacgt ccgaccgcgc cctgaccttc gagccgggcc agcccaacgt
      901 gtggatgttc gtgggcgtca acggggtcgg gaagaccacc agcatcggca agatcgccca
      961 gcgggaggcg gccgcgggcc gacgcgtcgt catggccgcc gccgacacct tccgggccgc
     1021 cgccgccgag cagctccagc tgtggtcgca gcgggtgggg gccgacctcg tccgggggca
     1081 ggatggcgcc gatcccggct cggtcgtctt cgacgccatg tcggccgccg caaaccgggg
     1141 tgccgacctc gtgttggtcg acaccgccgg gcgcctccac accaaggtca acctgatgga
     1201 ggagctgaag aagctgcgcc gcatcgtcga ccgcacgccg ggggcgctca aggaggtgct
     1261 gctggtcatc gacgccagca ccggccagaa cggcctcaac caggccaaga tcttcggcga
     1321 agcggtcgac gtaaccggcg tcatcctcac caagctcgac ggcaccgcca agggcgggat
     1381 cgtgctggcg gtccagaccg agctcggcat tccggtgaag ctggtcgggg tgggggagag
     1441 cgccgaggat ctgatcgagt tcgaccccga agagttcgtc gacgccctgt tcgccgcctg
     1501 atggtcgccg cctgatgttc gattcactct ccgatcggtt cgatgcgatc ttcacccgca
     1561 tgcgcggcaa gggccgcctg ggggagaagg aggtcgacga gatcgcccgg gag
//
LOCUS       DASWGG010000395         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_561250, whole genome shotgun sequence.
ACCESSION   DASWGG010000395 DASWGG010000000
VERSION     DASWGG010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561250"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /gene="meaB"
                     /locus_tag="VF954_07250"
     CDS             complement(<1..767)
                     /gene="meaB"
                     /locus_tag="VF954_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX9530916.1"
                     /translation="MGKARDRDPAALVEAAGAGDRTAVARLISLVERGGEAARAVGRL
                     AYPRGGQAYTVGITGAPGAGKSTLTDTLITEIRQDGSEVAVLAVDPSSPFSGGAILGD
                     RVRMQDHALDHGVFIRSMASRGHLGGLALAVPEAVRVLDAAGLPIVLIETVGVGQVEV
                     EVAGTADTTVVVVNPGWGDAVQANKAGLLEIADVFVINKADRPGAADTRRDLELMLML
                     GDVERAWTPPIVLATATKGDGTAELWSAIGGHRHHLES"
     gene            complement(781..>1820)
                     /locus_tag="VF954_07255"
     CDS             complement(781..>1820)
                     /locus_tag="VF954_07255"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX9530917.1"
                     /translation="AENHGNVTGRGNTLYMTHMTHMTVEHHDRYQRLVALGGAQLGFV
                     TAAQGRAAGWSLPQLRRAVEGGYLRRAGPCVYALPGAALPGQDLNGEDRRTLASDAIL
                     ACLCAGTGSVASHRTAAALHRLDGVSGPRRPDVTVAGRRSPRYRDATVHRPMDLAPGD
                     TTTVADVPCTKVPRTLIDLGAVCPDDVVERAVECALRRREVSIGYLWRRLERAARRGR
                     PGVGAMRRVLVQRAELPATDSDLESVCVQLIRDELGIEPDRHVQVLPGVIVDVKIPGR
                     PVVVEVDGYEFHSGRRQLLHDLERQNRLVTAGFVVVRFGYEHVYLRRHRELVAQTIAA
                     AADLEKRSRRR"
BASE COUNT          257 a          682 c          627 g          254 t
ORIGIN      
        1 gactcgaggt ggtggcggtg cccgccgatg gcggaccaca gctcggcggt gccgtcgcct
       61 ttggtagccg tcgccagcac gatgggcggc gtccaggccc gctcgacgtc gcccagcatc
      121 agcatcagct ccaggtcccg gcgggtgtcg gccgcgcccg gccggtcggc cttgttgatg
      181 acgaacacgt cggcgatctc cagcaggcct gccttgttgg cctgaacggc atcgccccat
      241 cccgggttca ccaccacgac cgtggtgtcc gcggtgcccg ccacctcgac ctccacctgg
      301 ccgaccccga ccgtctcgat gagcacgatc ggcaacccgg cggcgtccag gacacggacg
      361 gcctcgggca ccgcgagggc cagccccccc aggtgccccc ggctggccat cgaccggatg
      421 aacaccccgt ggtccagggc gtggtcctgc atccgcaccc ggtcgccgag gatggcgccc
      481 cctgagaacg gcgaggacgg gtcgaccgca agcaccgcca cctcggagcc gtcctggcgg
      541 atctcggtta tgagcgtgtc ggtgagcgtc gacttgcccg cgccgggcgc acccgtgatg
      601 ccgacggtgt aggcctggcc ccctcggggg taggcgagcc gcccgacggc ccgggcggcc
      661 tccccgcccc gctccacgag cgagatcaac cgggcgactg cggtgcggtc gcccgcgccg
      721 gccgcctcca cgagcgcggc cgggtcacgg tcgcgagcct tgcccatcac gaggccagtc
      781 tcaccgacga cgggaccttt tctccaaatc agcggcggcg gcgatggtct gcgcgaccag
      841 ctcccggtga cggcggaggt acacgtgctc gtatccaaat cgcaccacga cgaagccggc
      901 ggtcacgagc cggttctgcc gctcgaggtc gtggagcagc tgcctccggc cgctgtggaa
      961 ctcgtagccg tcgacctcca ccaccaccgg ccgcccgggg atcttcacgt ccacgatcac
     1021 gcccggcagg acctggacat ggcgatcggg ctcgatcccg agctcgtcgc gtatcagctg
     1081 gacgcagacc gattccaggt cgctgtccgt cgcgggcagc tcggcgcgct gcaccaggac
     1141 ccggcgcatg gcaccgaccc ctggccggcc acgccgggcc gccctctcca agcgccgcca
     1201 caggtagccg atggagacct cgcggcgccg gagggcgcac tccacggccc gttccacgac
     1261 gtcgtcgggg cacacggccc cgaggtcgat cagcgtcctg ggcaccttgg tgcaggggac
     1321 gtcggccacc gtggtcgtgt caccgggcgc gaggtccatc ggccggtgga cggtcgcgtc
     1381 cctgtacctg ggcgagcgtc gtcccgccac agtgacatcc ggccggcggg gcccgctcac
     1441 gccgtcgaga cggtgcaggg cggcagccgt tcgatgagag gcgaccgacc cggtgccggc
     1501 gcacaggcac gccaggatgg cgtcggacgc gagggtccgc cggtcctcgc cgttgagatc
     1561 ctggcccggg agggccgcgc ccggcagcgc gtagacgcaa ggccccgccc gccgcaggta
     1621 gccgccttcc acggcccggc gaagttgcgg tagcgaccac ccggcggccc ggccttgtgc
     1681 cgcggtgacg aatccgagct gcgcgccgcc cagggccacc aggcgttggt atctatcatg
     1741 atgttccacc gtcatatgag tcatatgagt catataaaga gtattgcccc ggcctgtgac
     1801 gttcccgtgg ttctcggcag
//
LOCUS       DASWGG010000396         6006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_233233, whole genome shotgun sequence.
ACCESSION   DASWGG010000396 DASWGG010000000
VERSION     DASWGG010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233233"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VF954_07260"
     CDS             complement(<1..543)
                     /locus_tag="VF954_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9530918.1"
                     /translation="MALGQRVATRATGRLVTENLTVGYSGVPVVSGVSIEVHPGEVVA
                     LLGRNGAGKSTLLHTLAGIIPPISGQLDVCGSGAGAPFHRRVRRGLGLVTEERAIIRR
                     LTVAENLRLAPGPAEDAERRFPELVALRGHKAGLLSGGEQQMLALAKALAARPTVLLI
                     DELSLGLAPLVLQRLLREVRA"
     gene            complement(543..3338)
                     /locus_tag="VF954_07265"
     CDS             complement(543..3338)
                     /locus_tag="VF954_07265"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HEX9530919.1"
                     /translation="MTALQLAADGLIFTGILILLGQSIVVVHRGSGVINFGSAAVGMV
                     SAYFFYDMWPGHGVPWPVALVIALGIAAVIGAAMYLLVLRQLRRSSVAMKVIATLGLM
                     LLASALTTQYFAPTGDVRSVRPFLPHRQLSPIHNLHFAEMGPILVGIGLAVTALLWLG
                     QKYTRFGLATSAVCENETVASGMGLSPHAISTANWALGSSLGALAIILIAPVSGLTPQ
                     TIPLLIVPALGAALIGRFDSLGLTLAGALAIGIGEAEVGVFTTGPGWAEAAPLIAIVA
                     VLIMRRPPRFDRSDAAARLPHVGTGRISGFALIAGAVSLLAVLTVSLTWLNPLAFSIL
                     IAIVTLSVVVITGYAGQLSLAQFGLAGLGAFFTALFSAGAGLPMWASILLGVVVTVPV
                     GLVIGVPALRVKGPTLAIATLSLVVVIEDLLLNNPSTVKWFKGGALPPLEIFGYSFNN
                     IAQPRRFAVLCWVVLVLIALATSNLRRSALGRRLLAIRTNPQAAEALGISPVAMKIYA
                     FTVASVMAAVCGALVEALSPYTDFTLFGTLQSITVVLSTTIGGLGMIPGALFGGGSFA
                     GGLATKVLSLIFRPSNWLDVIGGAGVLVVVLQAPDGVATGMQEQVASIRARVRRMRKR
                     PPAAGRPDAFASALAADRVRPGRRQPVRLDADGLSVTFGGVRALSGASLTVAPGQIVG
                     LIGPNGAGKSTFIEAVCGSTRAAGGTVRLDSEDIRGLSATHRARRGLARSFQSLELFE
                     DMTVGENLLAACEETTWSHGVRDLFWPRKVTVSEAAARAARDFGLEGVLAQTPRNLDH
                     GRRRLVAIARAYASDPAVLLLDEPAAGLDAQERNQLGSTLRKVASEWNVGILLVEHDV
                     DMVFRVCDRVTALVAGVPVAAGTPAEVRAHPAVTEAYLGRAEDTEPAVAVATENGAAG
                     TGPSPVQTAVVREGGKG"
     gene            complement(3486..4721)
                     /locus_tag="VF954_07270"
     CDS             complement(3486..4721)
                     /locus_tag="VF954_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9530920.1"
                     /translation="MGAALVAAGCGSGSSKPAANASFTLEYISDATGNQGYDIPTNTA
                     FAAAVKAVNARGGIKGHLINVLNCDTTSSANGSAACGQKAVSDHAFAIISFASSGAYL
                     PLAQQAGIPVLSEGITTESWTSPVSFMLSNGLIGGSAGYVALLKSQGCQHIGMVATVA
                     PGSAKNIQSLSDSMATEAKALGVDYKGYVSAPITAPDMAPYATQAVDKGYECLIPLAF
                     GPGAVSMMTSVNSLVQAGKFKKVGICICSITSQVRQALTPPIQAMGDAAVLSLSIESG
                     QDTANPAVRQWAKDMADYNGGTSLEFQGGMQWAELQLLVHAGDAVYPNVTAAKVLNYL
                     NGLHSYWPGISPPVDLSKAPANPYGPRVFASWVAPTKFTDGINFTRTAPFVSILTDQT
                     NDNPVPAKCNNWVAGACAS"
     gene            complement(4877..5035)
                     /locus_tag="VF954_07275"
     CDS             complement(4877..5035)
                     /locus_tag="VF954_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530921.1"
                     /translation="MTDAAVIIDQPDARHDLTRGAMALGGHQPSWISREPLTSGASST
                     PPIGALRT"
     gene            5164..>6006
                     /locus_tag="VF954_07280"
     CDS             5164..>6006
                     /locus_tag="VF954_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9530922.1"
                     /translation="MDTRVFGHRLRFARRKAGLTLEQLGQTVGHPPSYLSLLENGHRE
                     ARVSTVAQLADALGCTAADLLDPAPPSRRVELEVALAEMQEQPGYQQLRLPYLRPSAR
                     LDDTALEHIVTLFQTLQTVAPDCRDQPRGDQVRGDRPRRAATNAADQPVARRCVLANA
                     TMRRLMRDQDNYFGEIEKAAGAALDATGYPGTGALSERKLTDLAGYFGFTLARVKDLP
                     PSAQSITDVRERILYVPQRNTASTRNARSVICQTLGHFALGHHDPVDFEGYVRQRVEA
                     NYFAG"
BASE COUNT          932 a         2083 c         2171 g          820 t
ORIGIN      
        1 cgcccgcacc tcccgcaaga ggcgttgcag cactagcggg gccagaccga gggagagctc
       61 gtcgatgagc aggaccgtcg gccgggcggc cagggccttg gccagggcca gcatctgctg
      121 ctcgccgccg gagagcaggc cggccttgtg gccgcggagg gcgacgagct cgggaaagcg
      181 ccgctcggcg tcctccgccg gtccgggcgc gaggcgcagg ttctcggcca ccgtcagccg
      241 gcgaatgatg gcccgctcct cggtgaccag gccgagcccg cgccgtacgc gccggtggaa
      301 gggtgccccg gcaccgctcc cgcacacgtc gagctggccc gagatgggtg ggatgatccc
      361 ggccagggtg tgcagcaggg tcgacttccc ggcgccgttg cgaccgagga gggcgaccac
      421 ctcgccggga tgcacctcga tggacacgcc cgataccacc ggcacgcccg agtagccgac
      481 cgtcaggttc tcggtgacca gcctgcccgt ggccctggtg gcgactcgct ggccaagggc
      541 catcagccct tgcctccctc tcgcacgacc gcggtctgca ccgggctggg gccggttccg
      601 gccgcgccgt tctccgtggc gacggccacc gctggctcgg tgtcctcggc ccggcccagg
      661 taggcctcgg tgacggcggg gtgggcccgc acctccgccg gggtgcccgc cgccaccggg
      721 acgccggcca cgagggcggt cacccggtcg cacacccgga acaccatgtc cacgtcgtgc
      781 tccaccagga ggatcccgac gttccactcc gacgccacct tccgcagcgt cgagcccagc
      841 tggttgcgct cctgggcgtc gaggccggcg gcgggctcgt cgaggagcag cacggccgga
      901 tccgaggcgt aggcccgggc gatggcgacc agccggcgcc gtccgtggtc caggttgcgc
      961 ggcgtctggg cgagcacccc ctccagcccg aagtcgcggg cggcgcgggc ggcggcttcg
     1021 gagacagtca ccttccgggg ccagaacagg tcccgaacgc cgtgcgacca ggtcgtctcc
     1081 tcgcaggccg ccagcaggtt ctcgccgacg gtcatgtcct cgaacagctc gagggactgg
     1141 aaggacctgg ccagcccccg ccgggcccgg tgggtggcgg agaggccccg gatgtcttca
     1201 ctgtcgagcc gcaccgtccc gccggctgcc cgggtcgacc cgcacaccgc ctcgatgaag
     1261 gtggacttgc ccgcgccgtt gggacctatc agcccgacga tctggcctgg agcgacggtg
     1321 agcgaggcac cgctcagggc ccgcaccccg ccgaaggtca cggagaggcc gtcggcatcg
     1381 aggcgcaccg gctgccgccg gcccggacgc acccggtccg ccgcgagcgc gctggcgaag
     1441 gcgtcgggcc gcccggcggc gggggggcgc ttgcgcatcc ggcggacgcg ggcccggatg
     1501 gacgccacct gctcctgcat cccggtggcg acgccgtcgg gagcctggag caccaccacc
     1561 aggacgccgg cgccgccgat gacgtcgagc cagttcgagg gcctgaagat cagcgagagg
     1621 accttggtcg ccagcccacc ggcgaagctc ccgccgccga acagagcgcc cgggatcatt
     1681 cccaggccgc cgatcgtcgt cgacagcacc accgtgatcg actggagcgt gccgaacagg
     1741 gtgaagtcgg tgtaggggga gagcgcctcc acgagggcgc cgcagaccgc ggccatgacg
     1801 ctggccacgg tgaaggcgta gatcttcatg gccaccgggg agatccccag cgcctcggcg
     1861 gcctgtggat tggtgcgtat cgccagcagc cgccgtccca gggcgctgcg ccggaggttg
     1921 gaggtggcca gggcgatcag aacgaggacg acccagcaca gcaccgcgaa gcggcgcggc
     1981 tgggcgatgt tgttgaacga gtagccgaag atctcgaggg ggggcagcgc gccgcccttg
     2041 aaccacttga ccgtgctcgg gttgttgagc agaaggtctt cgatcacgac cacgaggctc
     2101 agcgtggcga tggccagcgt ggggcctttc acccgcagcg cggggacccc gatgaccagc
     2161 ccgacgggca ccgtcaccac gacaccgagg agaatcgagg cccacatcgg gagccccgca
     2221 cccgccgaga acagggcggt gaagaaggcg ccgagcccgg ccaggccgaa ctgcgcgagc
     2281 gagagctggc cggcatagcc ggtgatgacc accaccgaga gggtgacgat ggcgatgagg
     2341 atcgagaacg ccagagggtt gagccatgtc agcgacaccg tgagcacggc cagcagcgag
     2401 accgcgcccg cgatcagggc gaacccgctg atgcgccccg tcccgacgtg gggcagccgg
     2461 gccgcggcgt cggaccggtc gaagcgcggc ggccggcgca tgatcagcac ggccacgatg
     2521 gcgatgaggg gagcggcctc cgcccagccg gggccggtgg tgaacacacc gacctcggcc
     2581 tcgccgatgc cgatggcgag agcgcccgcg agggtcaggc cgagcgagtc gaaccggccg
     2641 atcagcgccg cccccagggc cggcacgatc aggagcggga tggtctgggg cgtcaggccc
     2701 gagaccggag ctatgaggat gatcgccagc gcgccgagcg acgacccgag ggcccagttg
     2761 gccgtggaga tggcgtgcgg cgacaggccc atgccggagg cgacggtctc gttctcgcac
     2821 accgcgctcg tcgccaggcc gaaccgggtg tacttctgcc cgagccacag cagtgcggtt
     2881 acggccagac cgatcccgac gaggatcggc cccatctcgg cgaagtgcag gttgtggatc
     2941 ggggagagct gcctgtgggg caggaagggc cgcaccgacc ggacatcgcc cgtcggcgcg
     3001 aagtactgag tcgtcagcgc gctggcgagg agcatcaggc cgagggtggc gatgaccttc
     3061 atagcaacag aggacctgcg aagctgccgg agcaccagga ggtacatggc cgcgccgatg
     3121 accgcggcga tgcccagcgc gatgaccagc gcgaccggcc acgggacgcc gtggcccggc
     3181 cacatgtcgt agaagaagta cgccgacacc atgcccacgg ccgctgagcc gaagttgatc
     3241 acgccgctgc cgcggtgcac cacgacgatg ctctggccca gcaggatgag gatgcccgtg
     3301 aagatcaggc cgtcggcggc cagctggagg gcggtcacgc catccccgcc agcgacatca
     3361 ggcgggggcc caaggcctgg gcggagggcc gaagccctcc gcccagctca acactttgct
     3421 gtgtcggcat cgctaccgtg cgtcgccggg cacccgactt gcgatgtcat gcgctcggcc
     3481 aacggtcacg atgcgcatgc gccggccacc cagttgttgc acttcgccgg aacggggttg
     3541 tcgttggtct ggtcggtcag gatggagacg aacggtgccg tgcgcgtgaa gttgatgccg
     3601 tcggtgaact tcgtcggggc cacccatgag gcgaagacgc ggggaccgta cgggttggcc
     3661 ggcgccttgc tcaggtccac cggcggcgag atgccgggcc agtacgagtg caggccgttg
     3721 aggtagttga ggaccttggc ggcggtgacg ttggggtaga ccgcgtcgcc ggcgtggacg
     3781 aggagctgga gctccgccca ctgcatgccg ccctggaact cgagcgacgt gcccccgttg
     3841 tagtccgcca tgtccttcgc ccactgcctc accgcggggt tggcggtgtc ctgcccgctc
     3901 tcgatgctga ggctgaggac ggccgcgtct cccatcgcct ggatcggggg ggtcagcgcc
     3961 tggcgcacct gcgaggtgat gctgcagatg cagatgccga ccttcttgaa cttgcccgcc
     4021 tggaccaggg aattcaccga ggtcatcatg ctgacggccc cagggccgaa ggcgaggggg
     4081 atgaggcact cgtagccctt gtctacggct tgcgtggcgt aaggcgccat gtcaggagcg
     4141 gtgatgggtg cgctgacgta gcccttgtag tcgacaccga gggccttggc ctccgtggcc
     4201 atggaatcgg acagcgactg gatgttcttg gcgctgccgg gcgccaccgt ggcgaccatg
     4261 ccgatgtgct ggcagccctg cgacttcagc agggccacgt agccggcaga gccgccgatc
     4321 aggccgttgc tcagcatgaa cgacaccggg ctggtccagc tctccgtggt gatgccctcg
     4381 ctgaggaccg ggatgccggc ttgctgggcg agcggtaggt aggcgcccga cgaggcaaag
     4441 gagatgatgg cgaaggcgtg gtccgacacc gccttctggc cgcacgccgc ggagccgttg
     4501 gcgctcgacg tggtgtcaca gttgaggacg ttgatcaggt ggcccttgat gccgccccgg
     4561 gcgttgaccg ccttcaccgc ggcggcgaag gcggtgttcg ttggtatgtc gtagccctgg
     4621 ttgcccgtgg cgtccgagat gtactcgagg gtgaagctgg cgttggcggc cggcttcgac
     4681 gagccggacc cgcagccggc cgcgaccagc gcggcgccca ccacagcggc gatgagcgcc
     4741 cggcggctcc tggatgacgt gcttcctgac gtggttcctg acacggcgag gtcccccctc
     4801 gtgttggttc ggatatcgga acggtaggag cccatgcgcc cgatcgcagg gatgggccgg
     4861 cttcccccgg aactgctcac gtacgcaacg ccccgatcgg gggagtcgac gaggcgcccg
     4921 acgtgagcgg ttcccggctg atccagctgg gctggtgccc gccgagcgcc atcgcccccc
     4981 gcgtcaggtc gtgcctggcg tcgggttggt cgatgatcac agcggcgtca gtcatgaaac
     5041 ccctcacacg tagcgtccct cacaggatgt caaggacgat aaaccttgtc gacgtcaaca
     5101 tcggcgtctc ttgtctgaag cgcgaagaac cgttatagtt ctgcgtccat gcgggctcgg
     5161 tctgtggaca cgcgggtctt cggtcaccgg cttcggttcg cccgccgcaa ggccggcctg
     5221 accctcgagc agctcggcca gaccgtcggc cacccgccct cgtacctgtc cctgctggag
     5281 aacggccacc gggaggcccg ggtgtcgacg gtggcacagc tggccgacgc gctgggctgc
     5341 acggcggccg acctgctgga cccggcgccg ccgagccggc gtgtcgagct cgaggtggct
     5401 ctggccgaga tgcaggagca gcccggctac caacagctgc gcctgccgta cctgcgcccc
     5461 tcggcgcgcc tcgacgacac ggcgttggag cacatcgtca cgctgttcca gaccctccag
     5521 accgtggcgc cagattgccg cgaccagccg cggggcgacc aggtgcgggg cgaccggccc
     5581 cggcgggcgg ccaccaacgc cgcggaccag ccggtggcgc gccgctgcgt gctggccaac
     5641 gccaccatgc gccgtctgat gcgcgaccag gacaactact tcggcgagat cgagaaggcg
     5701 gcgggcgccg cgctcgacgc cacgggctac cccggcaccg gcgcgctgtc ggagcgcaag
     5761 ctgaccgacc tggcgggcta cttcggcttc accctggccc gggtgaagga cctcccgcca
     5821 tcggcccagt cgatcaccga cgtccgcgag cgcatcctct acgtgcccca gcgcaacacc
     5881 gccagcaccc gcaatgcccg ctcggtcatc tgccagaccc tcggccactt cgccctcggc
     5941 caccacgacc cggtggactt cgagggctac gtccggcagc gggtcgaggc caactacttc
     6001 gccggc
//
LOCUS       DASWGG010000397        10437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2175703, whole genome shotgun sequence.
ACCESSION   DASWGG010000397 DASWGG010000000
VERSION     DASWGG010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175703"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1934)
                     /locus_tag="VF954_07285"
     CDS             complement(120..1934)
                     /locus_tag="VF954_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9530923.1"
                     /translation="MTSALRFGQIVVFAALGLVCLRIWRRQRGAAGAWLAVTFGDLAA
                     VSLVELALPQRLAHALGPTAAEHWVLKVVLLAIFVFPYPLFRFVAELEPAPARRRRLA
                     LVLTVAVAAWSLALPRILASADHRRPWWIVVLLILLVAEWATLASMVAVQLWKAGRHQ
                     PNGVRRRMQLLAGAALLLIAVLAPAASTSNADTTSGAHLASGLIGLTTAVLFYLGFAP
                     PAILRATWRRREERALVRAEMALMSVYSPDDVARSILPAVAAIFAARRCALVDPDGAP
                     MGVHGMAAADLEDWVGALRSVPGEAGLSSIKPGVVAFGLRHGRLVMETGSYTPLFGED
                     ELGLLSTLGAFIDLAMERAELSAKERDTREELERAHAELESLVHTFSHDLKSPLISLV
                     GYLDFLRSDYGDRLDETGLHYLDRMGASATYMSELIHDLLELSRIGRTDTEPEDVALE
                     PLLRDVAEAMHSAYPAARVEIGALPTVRVNGLRARQLFTNLCENAARHCGRRDAVINV
                     MCRPSEGGAVTIVVSDNGKGVPPEHRERVFGIFERLDPPGSASGTGMGLAICRKIVGQ
                     VHGRISIGDDGDGPGGGATFLIELPAALLRPRPALSRR"
     gene            2106..2912
                     /locus_tag="VF954_07290"
     CDS             2106..2912
                     /locus_tag="VF954_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9530924.1"
                     /translation="MTTDEVMVRRGGHVTTLTLNRPERLNAISGPMLHQLSTCLLEAD
                     ADRDVRAIVITGAGRGFCAGLDLQDASAGTGIGAGGGAGAGAGGNELDLRNAPPIVLH
                     EIDTPTICALNGGAAGYGMDLALGCDIRLAGESGKLAAAFTRRGVLPESGGTWLLPRL
                     VGWAKAAEIIFTGRTLPAKECLDLGLVSSVVADDALAEAAAALAGEIAANAPLAVRAS
                     KRMMRAGLDEAFANHVHHVFLELLPLFRSRDFQEGMASFLEKRPADFQGR"
     gene            3061..3966
                     /locus_tag="VF954_07295"
     CDS             3061..3966
                     /locus_tag="VF954_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9530925.1"
                     /translation="MGTMCEGRICIVTGGGRGIGREHAVMLAEQGAKVVVNDLGAEPD
                     GTGSSAGPAGEVVDKIREMGGEAVANGDDVSSWEGAQRLVNTAVETFGDLHVLVNNAG
                     ILRDRMLVNMTEEEWDAVIKVHLKGTFAPSRWAAAYWRERAKEGRANDARIINTTSTS
                     GIYGNAGQTNYGAAKMGIAGFTIIAARELGRYGITVNAIAPGALTRLTEALRREAPTE
                     EQRKAASPRWIAPLCTWLASPASASVTGRVFEASGQVLGVAEGWHRGPTTSPVAEPED
                     IGDELLALVSKARPNAAMNGQDERV"
     gene            complement(4022..4471)
                     /locus_tag="VF954_07300"
     CDS             complement(4022..4471)
                     /locus_tag="VF954_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530926.1"
                     /translation="MEFATDEVGEVVALMDELADGSGWINIRPAVDPDDLPPNGGRPT
                     FISAVGPLVPLCTWTPAQLGRRPTPPLIGIQHAAGGKAAPELGSLGVAVPDGWRVVQD
                     ESRRGLVVAVAPSLPSGPVLAWLLAAGGALCSVPFSGWVAAVYRRTR"
     gene            complement(4489..5871)
                     /locus_tag="VF954_07305"
     CDS             complement(4489..5871)
                     /locus_tag="VF954_07305"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEX9530927.1"
                     /translation="MRRSRPGRLRTGALLLATTVPAGMAVAVVVPGGPRARAQPFAGG
                     GVITFGDAGYYGSPTDTMLTAPVTGMAATPDGRGYWLSAADGGVFAYGDAGYFGSAGG
                     IDLDAPVIGVTGTPDGGGYWQLAMDGGIFSYGDAAFHGSTGNLILNRPVVGMTPTPTG
                     KGYWLVAGDGGIFAFGDAQFYGSTGALKLNQPVVGMAATATGRGYWLVAGDGGVFPFG
                     DAGFYGSAGGQPIAAGIVGLAPSPDGRGYTMAGGDGAIYTFGDARSFGSDAAVVPTPP
                     ITAFQVTPDGQGYWLLDRDAFATGFSRPAATVTNGVGPAIVRISAAEVGGDPDGGPFC
                     NPYGPCEEWCSLFATWAWERAGVAIPHYPFTGDIWTWATQRGTALPPTARPAGGDLVL
                     YGTGPANTATSVHVGIVAEVWPDGAITTVEGDAGPGPHGRANVIINGPFLPSQSSSYN
                     GFPIYGYAAP"
     gene            complement(5873..6946)
                     /locus_tag="VF954_07310"
     CDS             complement(5873..6946)
                     /locus_tag="VF954_07310"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9530928.1"
                     /translation="METTAAATTTSTGGDVLAFAPAVMAGSLAGRTLNAPVVAMAATP
                     TGKGYWLATSDGGVFSFGDAVFYGSAGSVRLNRPIVGLAATPTGRGYWLVASDGGIFS
                     YGDATYSGSGAGTALARPVIAVVASPGGGYWMAEGQRGLVGHVVVIDPGHNGGNGAHP
                     EIINQPVDIITGTKPCDTAGTATNDGYPEFAYNFDVASRASQLLQQAGATVILTHPDD
                     LGVGPCITERAAIGNRADAAVAISIHADGGPPTGRGFHVIQPAPIAGHNTDIVGPSDQ
                     FATELRDLFLAETGMPTSDYIGEDGLDTRDDLGGLNLSTVPKVFIETGNMRNATDAGL
                     LEDPAFRQRAAQAISDAMGELLS"
     gene            complement(7131..7382)
                     /locus_tag="VF954_07315"
     CDS             complement(7131..7382)
                     /locus_tag="VF954_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530929.1"
                     /translation="MKKLADHGFDDHPEDDPERARLAWAVVAIDDCEECEDLRVELTV
                     EEAGRPGTGLVGHLSPASARRLRAALATALREIGEPPDG"
     gene            complement(7409..8395)
                     /locus_tag="VF954_07320"
     CDS             complement(7409..8395)
                     /locus_tag="VF954_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530930.1"
                     /translation="MSIRLEPQDEYMHELGPELNFNESMYFNLYDPRARLGGFFRLGN
                     RANEGTAEMTVCLYLPDGRVGFMFQRPKITNNDAFNSGGLAFEVIRPFEELEVSYGGK
                     VVLLDDPLAVADPKRAFTENPSIDCSVKLTYTRVSTMFGGESDQPTEAAGEEFARGHY
                     EQLVAGVGTISVGEQTWEVEGYGLRDHSWGPRYWQAPWFYRWLTANFGPNFGFMASRS
                     TRRDGKARTGGFLWDGTGMHIVDHVEISTIWSGEDSYHAEIEAVLRRGDNETRVKGRV
                     INLVPLRNRREDLVTRISEGMTEWTLEDGQVGYGLSEYLDQIIDGKPVGVAS"
     gene            complement(8579..9181)
                     /locus_tag="VF954_07325"
     CDS             complement(8579..9181)
                     /locus_tag="VF954_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX9530931.1"
                     /translation="MTRSFEVTWDYRCPFARNAHEHVVAGLRAGEDWDVHFVPFSLEQ
                     VHVAEGDPDIWGRAELPSGVPAMLAGIVVRDRVPERFLEVHTALFRARHDEGIDLRDA
                     TNVDAVLADAGVDAASVRAEIDAGWPLEVFRKEHEAAAGDHTVWGVPTFVRGSESVFV
                     RLMTRPEGDSSLAARTVNRLLDLMEGFPELNEYKHTTIRR"
     gene            complement(9241..9801)
                     /locus_tag="VF954_07330"
     CDS             complement(9241..9801)
                     /locus_tag="VF954_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9530932.1"
                     /translation="MPLFRFAEGPRQRVADFDKAVDRAFDHLRGHKSADRLFYTASEL
                     GDHGLIWLVLGALRGLRSDRDWKAALRAAAGVGIESAVVNLGVKSLFRRSRPAFDGSR
                     PRRLRQPRTSSFPSGHATSAFSAAALLSEDDALWPVYYGVAVVVAASRVYVRVHHASD
                     VLAGAALGAALGRIGRRMAPLPRRPP"
     gene            complement(9811..>10437)
                     /locus_tag="VF954_07335"
     CDS             complement(9811..>10437)
                     /locus_tag="VF954_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX9530933.1"
                     /translation="LQLDPEFAALELAEDQARAFRAEAASLLRRYFAIEDPNQVRAIG
                     VELLLEANLGDLRLRGIIDRLDLDADGELVVTDYKTGRVPPTGYEQRRLGGVHFYAYL
                     CERVLGRRPARVQLLYLSEPLVISTVPSEQSVRGLERQARAIWSAVRLACEREDFRPR
                     PSPLCASCGFHAYCPAMGGDVALVPRPAVPMAGAGAGTGTGSGAGTPR"
BASE COUNT         1504 a         3686 c         3826 g         1421 t
ORIGIN      
        1 gcatcggcga ccgcaaggtc cgggccgcgg aggccgaggc cctggagttg ctgcggtccg
       61 ccgacgtggt cgccgccctc gacgaccgcg ccgcgtagcg cccacccagg gggctgcggt
      121 caccggcgac tcagcgcggg gcgagggcgc aacaacgcgg ctggcagctc gatgaggaag
      181 gtggctccgc caccgggacc gtcgccgtca tcgccgatgg agatccgacc gtggacctgc
      241 ccgacgatct tccggcagat ggccagcccc atcccggttc cgctcgcgct gcccggcgga
      301 tcgagccgct cgaagatccc gaagacccgc tcccggtgct cgggcgggac gcccttgccg
      361 ttgtcggaca ccacgatggt gaccgccccg ccttcggacg gccggcacat gacgttgatg
      421 acggcgtccc ggcggccgca gtgtctggcg gcgttctcgc agaggttggt gaacagctgg
      481 cgggcccgca ggccgttgac ccggacggtg ggcagtgcgc cgatctcgac ccgcgccgcg
      541 ggataggcgc tgtgcatggc ctcggcgacg tcgcgcaaca gcggctcgag ggcgacgtcc
      601 tcgggctccg tgtcggtccg tccgatccgt gacagctcga gcaggtcgtg gatgagctcg
      661 gacatgtagg tggcggacgc gcccatacgg tcgagatagt gcagcccggt ctcgtccagc
      721 cggtcgccgt agtcgcttcg caggaagtcc aggtagccga ccagggagat cagcgggctc
      781 ttcagatcat gggagaacgt gtgcaccagg ctctccagct cggcgtgggc ccgttccagc
      841 tcctccctgg tgtcgcgctc cttggcggag agctcggctc gctccatggc caggtcgatg
      901 aaggcgccga gcgtggagag cagcccgagc tcgtcctcgc cgaacagcgg ggtgtacgag
      961 cctgtctcca tgaccagccg gccgtggcgc agcccgaagg cgacgacgcc gggcttgatg
     1021 gacgagagcc cggcctcgcc cggcaccgag cgcagcgcac ccacccagtc ttcgagatcg
     1081 gccgcggcca tgccgtgcac tcccatgggc gcgccgtcgg ggtcgacgag cgcacacctc
     1141 cgggccgcga agatggcggc gacggcgggc aggatggacc gggccacgtc gtccggcgag
     1201 tacacgctca tgagggccat ctcggcccgg accagcgcgc gttcctcccg gcgccgccac
     1261 gtggctcgca gaatggccgg cggggcgaac ccgaggtaga agagcacggc ggtggtgagc
     1321 ccgatgagcc cgctcgccag gtgggcgccg ctggtggtgt cggcgttgga ggtcgacgcg
     1381 gcgggggcga ggacggcaat gagcaggagg gcggcgccgg ccagcagctg cattcgccgg
     1441 cggacgccgt tgggctggtg gcggccggcc ttccagagct ggacggcgac catcgacgcc
     1501 agggtcgccc actccgccac cagcaggatg agcaggacca cgatccacca cggtcgtcga
     1561 tggtccgccg acgccaggat cctgggcagg gcgagggacc aggcagccac ggcgacggtc
     1621 agcaccagcg cgaggcgccg gcgccgggcc ggggcgggtt ccagctcggc gacgaaccgg
     1681 aagagcgggt aggggaatac gaagatcgcc agcaagacga ccttgaggac ccaatgctcg
     1741 gcggcggttg gccccagggc atgagcgagt cgctgcggca gcgccagctc gacgagcgag
     1801 acggcggcca gatcgccgaa cgtgacggcc agccatgccc cggccgcgcc gcgctggcgc
     1861 cgccagatgc gcaggcagac gagcccgagc gcggcgaaca ccacgatctg gccgaagcgc
     1921 agggcgctcg tcaactccct gattgccgct cacctcctgg tgtcgaagcg ctctctttgg
     1981 acggtaaggg tccggacggg gccgcgccat agaccggccg ggcggttcga catggcccga
     2041 agtgactgga cagatcgtgc catcggcgac cgtcgaggtg gtcgatcccc attagcgtcg
     2101 gcgtcatgac caccgacgaa gtcatggtgc ggcggggcgg ccacgtgacg accctcacgc
     2161 tcaaccggcc cgagcgactg aacgccatct ccgggccgat gcttcaccag ctctccacct
     2221 gccttctgga ggccgacgcc gaccgcgacg tgcgggccat cgtgatcacc ggcgccggcc
     2281 gcggcttctg cgccggcctc gatctccagg acgcgtccgc cgggacgggc atcggcgccg
     2341 gaggtggtgc cggcgcaggc gcgggtggca acgagctcga ccttcgcaac gcacccccga
     2401 tcgtcctgca cgagatcgac acgccgacca tctgcgcctt gaacggaggg gcggccggct
     2461 acgggatgga cctggcgctg ggctgcgaca tccggctggc cggggagtcc gggaagctgg
     2521 ccgccgcctt caccaggcgg ggggtgctgc cggagagcgg cgggacgtgg ctgctgccca
     2581 ggctggtcgg ctgggccaag gcggccgaga tcatcttcac cggccgcacg ctgccggcca
     2641 aggagtgcct cgacctgggc ctggtgagca gcgtggtggc ggacgacgcc ctggccgagg
     2701 cggcggcagc gttggcggga gagatcgcgg ccaacgcgcc gttggccgtc cgggcgagca
     2761 agcggatgat gcgcgccggg ctcgacgagg cgttcgccaa ccacgtccac cacgtgttcc
     2821 tcgagctgct gccgctcttc cgcagccgcg acttccagga gggcatggcg tcgttcctgg
     2881 agaagcgccc ggccgacttc cagggccggt agccgtttac cgttgagcca tgcggcggtt
     2941 gtcgcccagc cgtggtgagc cgcctctggc cgggccgttg gcgttggccg ccggctgatc
     3001 ccggccgcta tggtcctgcc ggctggcggg ctgggtggtc ctgatcgaag gaggacgctg
     3061 atgggcacca tgtgcgaggg acggatctgc atcgtcaccg gcggcgggcg gggcatcggg
     3121 cgggagcacg ccgtgatgct cgccgagcag ggggccaagg tcgtcgtcaa cgacctcggc
     3181 gccgagccgg acggcacggg atcctccgcc ggtcccgccg gcgaggtcgt tgacaagatc
     3241 cgggagatgg gcggggaagc ggtggccaac ggcgacgacg tgtcgtcgtg ggaaggcgcc
     3301 cagaggttgg tcaacaccgc ggtcgagacc ttcggcgatc tccacgtgct ggtcaacaac
     3361 gccggcatcc tgcgcgaccg catgctcgtg aacatgaccg aggaggagtg ggatgcggtg
     3421 atcaaggtcc atctcaaagg gaccttcgcc ccgtcccgct gggcggccgc ctactggagg
     3481 gagcgggcca aggagggcag ggccaacgac gcccgcatca tcaacaccac ctcgacgtcc
     3541 ggcatctacg gcaacgccgg tcagaccaac tacggcgcgg ccaagatggg catcgccggc
     3601 ttcacaatca tcgcggcccg ggagctgggc cgctacggga tcacggtgaa cgccatcgcc
     3661 ccgggcgcct tgacccgctt gaccgaggcg ctccggcgtg aggcgcccac cgaggagcag
     3721 cgcaaggcgg cgtcgccccg ctggatcgcc ccgctgtgca cctggctggc gtcgcccgcg
     3781 tcggccagcg tgacgggccg ggtgttcgag gcgtcgggac aggtgctcgg ggtggccgag
     3841 ggctggcacc gggggcccac cacctcgccg gtcgccgagc cggaggacat cggcgacgag
     3901 ctgctcgccc tggtgtccaa agcccgcccc aacgcggcca tgaacggcca ggacgagcgg
     3961 gtgtaggccc acccggccgg ccggccggct ggctggcccg ggctcccgtc cgcccggccg
     4021 gctatcgggt ccggcggtac acggccgcga cccacccgct gaacggcacg gagcagaggg
     4081 cgccgccggc cgcgagcagc caggccagca cggggcccga gggcagcgat ggcgccacgg
     4141 cgacgaccag gccgcgacgg gactcgtcct ggaccacccg ccaaccgtcc ggcacggcga
     4201 ctccgaggct ccccagctcg ggcgcggcct tgccgcccgc ggcgtgctgg atgccgatca
     4261 gcggcggggt gggccgccgg ccgagctgcg caggggtcca tgtgcagaga ggcacgagcg
     4321 gcccgacagc cgagatgaac gtcggccttc cgccgttcgg cggcaggtcg tcgggatcga
     4381 ccgcggggcg tatgttgatc cacccgctcc catctgcgag ctcgtccatc agcgccacga
     4441 cctcgcccac ctcatcggtc gcgaactcca ccagctgcgg ccggcgcctc acggcgcggc
     4501 gtacccgtag atcgggaacc cgttgtagct gctggactgt gatggcagga acggcccgtt
     4561 gatgatcacg ttggccctgc cgtggggccc gggaccggcg tctccctcga cggtggtgat
     4621 ggccccgtcc ggccacacct cggccacgat gcccacgtgc acggaggtcg ccgtgttggc
     4681 gggcccggtg ccgtagagca ccaggtctcc gcccgcgggc cgggcggtcg gcgggagagc
     4741 ggtccctcgc tgggtggccc aggtccatat gtcaccggtg aagggatagt gcgggatggc
     4801 gactcccgcc cgttcccagg cccacgtggc gaacaacgag caccattcct cgcacggccc
     4861 gtacgggttg cagaacgggc cgccgtcggg gtcgccgccg acctcggcgg ccgagatgcg
     4921 aacgatggcc gggcccacgc cattggtgac cgtcgccgcg ggccgggaga acccggtggc
     4981 gaaggcatcg cggtcgagca gccagtagcc ctgcccgtcc ggcgtcacct ggaaggcggt
     5041 gatcgggggg gtcggcacca ctgccgcgtc ggagccgaag gacctggcgt cgccgaaggt
     5101 gtagatcgca ccgtcgccgc cggccatggt gtagccccgg ccgtccgggg agggcgccag
     5161 gccgacgatt ccggccgcga tgggctgtcc gcctgccgat ccgtagaacc cggcatctcc
     5221 gaacgggaac acgccgccgt ccccggccac cagccagtac cccctgcccg tcgcggtggc
     5281 cgccatgccg acgaccggct ggttgagctt gagggcgcct gtggagccgt agaactgtgc
     5341 gtctccgaag gcgaagatgc ctccgtcgcc ggccaccagc cagtacccct tgccggtggg
     5401 tgtgggcgtc atcccgacca ccggccggtt gaggatcagg ttgccggtgg atccgtggaa
     5461 ggccgcatcg ccataggaga agatcccgcc gtccatggcg agctgccagt agcccccgcc
     5521 gtccggggtg ccggtgacgc cgatgaccgg cgcgtcgagg tcgatcccgc ccgccgagcc
     5581 gaagtagccg gcgtcgccgt acgcgaagac cccgccgtcg gccgcggaga gccagtagcc
     5641 gcggccgtcg ggggtggcgg ccatgcctgt aaccggcgcg gtgagcatgg tgtcggtcgg
     5701 gctgccgtag tagcccgcgt cgccgaaggt gatcacgcct ccgccggcga acggttgggc
     5761 ccgggccctg ggcccgcccg gcacgacgac cgcgaccgcc atgccggcgg ggacggtcgt
     5821 ggccagcagg agagcgccgg tgcgtagccg gccggggcgg gagcgtctca tgtcagctca
     5881 ggagctcgcc catggcgtcg ctgatcgcct gcgcggcccg ctgccggaac gcagggtcct
     5941 ccagcaggcc ggcgtcggtc gcgttgcgca tgttcccggt ctcgatgaac accttgggca
     6001 ccgtcgagag attgagcccg cccaggtcgt cccgcgtgtc gagcccgtcc tcgccgatgt
     6061 agtcggacgt cggcatcccc gtctcggcga ggaagaggtc gcgcagctcg gtcgcgaact
     6121 gatccgacgg gccgacgatg tcggtgttgt gcccggcgat cggagcgggc tggatcacat
     6181 gaaagccccg cccggtgggc ggcccgccgt cggcgtggat ggagatggcg accgccgcgt
     6241 cggcccggtt gccgatggcc gcccgctcgg tgatgcacgg cccgaccccc aggtcgtcgg
     6301 ggtgggtgag gatcaccgtg gcgcctgcct gctggagcag ttgcgaggca cgcgacgcca
     6361 cgtcgaagtt gtaggcgaac tcggggtacc cgtcgttggt cgccgtgccc gcggtatcgc
     6421 acggcttggt gccggtgatg atgtcgaccg gctggttgat gatctcgggg tgggccccgt
     6481 tgccgccatt gtggccgggg tcgatgacta cgacgtggcc cacgaggccc cgctggccct
     6541 cggccatcca gtacccgccg ccgggcgacg cgaccacggc gatcaccggg cgggccaggg
     6601 ccgtgccggc cccactcccc gagtaggtgg cgtcgccgta ggagaagatg ccgccgtccg
     6661 aggccaccag ccagtagccc cgccccgtcg gggtggcggc caggccgacg atcgggcggt
     6721 tgagccggac gcttccggcg ctgccgtaga acacggcgtc accgaaggag aagacccccc
     6781 cgtcgctcgt cgccagccag tagcccttcc cggtcggggt ggccgccatc gccacaaccg
     6841 gcgcgttcag cgtacgaccc gccagggacc cggccatgac ggccggggcg aaggccaaca
     6901 cgtcccctcc cgtcgaggtg gtggtcgcgg cggccgtggt ctccatttgg ccgggtgtag
     6961 cgacggcgac cgccaccgct acgcacagga cgatccccgc gcctgcggtc cgcatgtgtg
     7021 ccggtgccca ccgttgcatg gccgccctcc cgatggctgg cgacgattgt ctcatgccgt
     7081 ggggctggac gccgcagggc tacgggcgcg tcggcccttc gtccgagggc tcagccgtcg
     7141 ggcggctcac cgatctctcg cagggcggtg gccagcgcgg ctcgcagccg gcgcgccgaa
     7201 gccggggaga gatgcccgac cagcccggtg ccgggccggc cggcctcctc caccgtgagc
     7261 tccacccgca ggtcctcgca ctcctcgcag tcgtcgatgg cgaccaccgc ccatgccagg
     7321 cgggcccgtt cgggatcgtc ctcggggtgg tcgtcgaagc cgtggtcggc gagtttcttc
     7381 atggggcgcc tccagggctg cgctgccgct agctggccac cccgaccggc ttgccgtcga
     7441 tgatctgatc gaggtactcc gagagcccgt agccgacctg gccgtcctcg agcgtccatt
     7501 cggtcatgcc ttcggagatg cgcgtcacca ggtcctcccg gcggttgcgg agagggacca
     7561 ggttgatcac ccggcccttg acgcgtgtct cgttgtcgcc ccgccgcagc acagcctcga
     7621 tctccgcgtg ataggagtcc tcacccgacc agatggtgga gatctccacg tgatcgacga
     7681 tgtgcatgcc ggtcccgtcc cacaggaacc cgcccgtcct ggccttgccg tcccgccggg
     7741 tcgagcggga cgccatgaac ccgaagttgg gcccgaagtt ggcggtcagc cagcggtaga
     7801 accacggggc ctgccagtag cgcggtcccc aggagtggtc gcggaggccg taaccttcga
     7861 cctcccaggt ctgctccccg acgctgatgg tgccgactcc ggcgaccagc tgttcgtaat
     7921 ggccgcgggc gaactcctcg ccggcggcct cggtgggctg gtcggactcg cccccgaaca
     7981 tggtcgacac gcgcgtgtag gtgagcttca cgctgcaatc gatggacggg ttctcggtga
     8041 aggcccgctt cggatcggcg acggcgagcg gatcgtccag gaggacgacc ttgcctccgt
     8101 aggacacctc cagctcctcg aagggtcgga tcacctcgaa agccaggccg ccggagttga
     8161 aggcgtcgtt gttggtgatc ttgggccgct ggaacatgaa gcccacccgg ccgtcgggca
     8221 ggtagaggca gaccgtcatc tcggccgtgc cctcgttggc ccggttgccg agccggaaga
     8281 acccccccag gcgggcgcgg gggtcgtaca ggttgaagta catgctctcg ttgaagttga
     8341 gctccggccc cagctcgtgc atgtactcgt cttgtggttc caggcgaatc gacatgagtg
     8401 tcagcttagc tgagctccgc gccaatggca cagccgggca tcagggatcc gggcccgcgc
     8461 cgtccccgac gtggccgtcg gccccagacg gagctgctct gtccgcccgg cccgggcgag
     8521 gaggggctcc cacgtatcgc ttctcgcgcc catcggcatc gggctccacg gcgggccgtc
     8581 agcggcggat cgtggtgtgc ttgtactcgt tcagctcggg gaacccctcc atgaggtcga
     8641 ggagccggtt gacggtcctg gccgccagcg agctgtcgcc ctccggccgg gtcatgagcc
     8701 gaacgaacac cgactcggag ccccgcacga aagtgggcac tccccagacc gtgtgatccc
     8761 cggccgcggc ctcatgctcc ttgcggaaca cctcgagcgg ccagccggcg tcgatctccg
     8821 cccggacgct cgccgcgtcg acgcccgcgt cggccaggac ggcgtcgacg ttggtggcat
     8881 ccctcaggtc gatgccctcg tcgtggcggg cgcggaacaa cgccgtgtgg acctccagga
     8941 accgctcggg cacccgatca cgcaccacga tgcccgcgag catcgccggt acccccgacg
     9001 ggagctcggc ccggccccag atgtccgggt cgccttccgc gacgtggacc tgttcgaggg
     9061 agaacggcac gaagtggacg tcccagtcct ctccggcccg caggccggcc acgacgtgct
     9121 cgtgggcgtt gcgcgcgaac ggacagcggt aatcccaggt cacttcgaag gagcgggtca
     9181 tacctctccc aacatcacgg ccggatcggg gcattccccc tgcccggccc cctgcccggg
     9241 tcacggtggg cgacgcggga gcggtgccat ccggcggccg atgcggccca gggcggcgcc
     9301 cagggcggcg ccggccagca cgtcggaggc gtggtggacc cgtacgtaca cccggctggc
     9361 ggccaccacc acggccacgc cgtagtacac cggccagagg gcatcgtcct ccgagagcag
     9421 ggcggcggcg gagaacgccg aggtggcgtg gccgctcgga aagctgctgg tccgcggttg
     9481 ccggagccgg cggggccggc tgccgtcgaa ggccggccgg gaccgacgga acagcgactt
     9541 gacgccgagg ttcacgaccg cggactcgat gcccaccccg gcggccgccc ggagcgccgc
     9601 cttccagtcc cgatcggacc gcaggccccg gagggcgccg agcacgagcc agatcaggcc
     9661 gtggtcgccg agctcggaag ccgtgtagaa gagccggtcg gcggatttgt gcccgcgcag
     9721 atggtcgaag gcgcggtcca cggccttgtc gaaatcggcc acccgttgcc gcgggccctc
     9781 ggcgaaccgg aacagcggca tggccggacg ctaccggggg gtgccggccc cggagcccgt
     9841 gcccgtgccc gcgccggccc cggccatggg gacggcgggg cggggtacga gggcaacatc
     9901 gccacccatg gccgggcagt acgcgtggaa cccgcagctc gcgcacagcg gcgacggccg
     9961 gggccggaag tcctcgcgct cgcaggccag ccgcaccgcg gaccagatgg cccgggcctg
    10021 gcgctccagg cctcgcaccg actgttccga cggcaccgtg gagatgacca gaggctcgct
    10081 caggtacagc agctgcacgc gcgccggccg gcggccgagg acccgctcgc acaggtaggc
    10141 gtagaagtgg acgcccccga gccggcgctg ctcgtaaccg gtgggcggga cccggccggt
    10201 cttgtagtcg gtcaccacca gctcgccgtc cgcgtccagg tccagccggt cgatgatgcc
    10261 ccgcagccgc aggtcgccca ggttggcctc gaggagcagc tcgacgccga tggctcgcac
    10321 ctggttgggg tcctcgatgg cgaagtagcg tcgcaggagc gacgccgcct ccgcccggaa
    10381 cgcccgcgcc tggtcctccg ccagctcgag ggcggcaaac tcggggtcca actggag
//
LOCUS       DASWGG010000398         6287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_572353, whole genome shotgun sequence.
ACCESSION   DASWGG010000398 DASWGG010000000
VERSION     DASWGG010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572353"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VF954_07340"
     CDS             <1..699
                     /locus_tag="VF954_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX9530934.1"
                     /translation="AREGSPRTPRQQLRVELLGIAGLILLAQAFKVMIVEAAPPMWQV
                     MPSTLPGWIDLFAMGMALAVASAWTAEHGREPAVASARWAPWAAWALAAAAFWAVSTQ
                     IGLPRSPLYDATLSQYLARQALYGAFALFLLLPGIFGPQDRGLIRALLQNRAVQAAGL
                     ISYGIYLWHETLLTKFFVWFHRRNFQVSFPKLFLAILAATLATSAVTYLAVERPFLRM
                     KDRRLLRLREQRAL"
     gene            733..2019
                     /locus_tag="VF954_07345"
     CDS             733..2019
                     /locus_tag="VF954_07345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9530935.1"
                     /translation="MTSLDHDPAAAAPPPTGAPLPGAVPGLDLAELAASAGLRRIHIL
                     AWRDLDDPEAGGSELHAARIAERWAAAGLEVTMRSSHADGHPHYTRRDGYQVIRKAGR
                     YGVFPRTALSGLVGRTGDRDAMVEIWNGMPFFSPLWTRCPRVVFLHHVHAEMWRMVIH
                     PPALAKAGEAVELRLAPPVYRRSRIVTLSESSRQEIIDLLHLPAANIDVVPPGVEPRF
                     GPAPDPKRARSERPLVVAVGRLVPVKRFDLLIAALTELKVRHPDLEAVIVGEGYERPA
                     LEARRRAAGAEDWIHLPGHLEDDALVELYRKAWVLASTSAREGWGMTITEAAACGTPA
                     VVTRIAGHLDAVEEGGSGLLVEAGADPLAGALDAVLANPLLREKLSRGAVERAARLTW
                     EATARGTLAALVAEARRRRPAPPAAPAAPAAPPPGG"
     gene            2016..6278
                     /locus_tag="VF954_07350"
     CDS             2016..6278
                     /locus_tag="VF954_07350"
                     /inference="COORDINATES: protein motif:HMM:NF023275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HEX9530936.1"
                     /translation="MTGPERRHRDLGAGLAWLILAIPYLPLLLTHPGKVGADTKAYLY
                     LDPTRLLKEAPYLWDPKVAMGTVTHQNIGYLLPMGPWFAALHAAAVPIWVAQRLWLGT
                     LLLLAGAGVSCLLQTLGWDRRHTILAALAYELSPYLLLYEARTSAILLPWAGLPWMIA
                     LVIRAARADRRRWRDPALFAVVVALVGGTNATSLLFAGLGPALWLPFAVWSHQVKPGR
                     AVATALRIGVLSLGTSLWWIAGLMAQGTYGLNVLKYTETVPAVARTSYPYEMLRGLGN
                     WYFYGLDRVSYWVTPARYLTQTPLVIAASFAIPLLALASAAAVRWRERSYFMLLVAAG
                     LVIGVGVHPYHHPTPLGAAFKAFAQGSSAGLALRTTARAVPLLVLGTAVLLGAGVKAL
                     LEKSHGLLHRPPWRLAVPLACAALVAVAQPALWTGGFVESSLDHPETVPAYWAQAAAA
                     LTDAGGATRALDLPGADFSYYRWGATVDPAEPGLTTRPLVGRELTPFGSAASADLVRA
                     LDRRLQEGVLEPGAIAPVARLMGAGTVLVRNDLQYERFRTPRPRNVEELLTPTPPGLD
                     APTGFGPPASIPPRVTTNDEVTLGVPPATPYPPSVETFAVQDPAPIVRTEPAASPLLV
                     AGDGEGLVEAAAAGLLTGPGPVVYSGSAASDPSLVQGALASGADLLLTDTNRRRAQRW
                     GTVRENYGYTERPGEVPLVSDPSDARLPLFPGATDSAFTTAEQRGVSSVMATAYGDPV
                     AYTAADRPAMALDSDPATAWTVAAFDDPRGQKLVVTLAHPVTADHLTLLQPVLPPNER
                     YITQAHLIFDGGPAVPITLDPVASRRVPGETIPFPRATFSQLTFVIDQTNYGNRADFS
                     GVSGVGLAELTIGDPTLAQHVDEVVHLPTDLLGLAGPASAGHNLTVLLARARSNPAEP
                     YKGDEEQAVVRSFSLPAGRAFALSGEARLASAAPDSVLDAVRGQPSVFSASSRLPGDL
                     DARAEMAFDGSPSTAWQSSFGDVNAWVEADAPAPITVDHVDLGIVDDGVHSVPTQLTL
                     SVDGRPGPVLDVPRSGAVHLAFPSVNGSRFRLTVSGLREVTTVNYFSKTPQVTPVSLT
                     EVGLGGLRAPPPPPTIDTGCRPDLLTVDGRPYPVRVTGGTDAADRGQPLAVSPCGPAL
                     RLGPGDHLVRAAEGRLTGLNLDRLVLRSAAAAAPAPAGAAPPSAPAVHVTHDGATSVT
                     AKVTGAAGPFWMVLGQSHNAGWHATLAGTSLGASHLVDGYANGWLVRPPPGAAALTIH
                     MTWTPQRRVWQALAASGMVLLLCLLLGFRSGGTETVTSSLPVWRSPWSAELPALKGRT
                     VVAVAGVLAGGAVLAGPGIGIALAAVTAVAAVWPRARVLLTGGAVACVAVIAAYVLEL
                     QVRYAFPLKLSWPAHFQLVAPLGWAAVGLIVADGVLEWAREG"
BASE COUNT          705 a         2600 c         2103 g          879 t
ORIGIN      
        1 gcgcgggagg ggtcgccccg cacgccccgc cagcagctgc gagtcgagct gctcggcatc
       61 gccgggctga tcctgctcgc ccaggccttc aaggtgatga tcgtcgaggc cgccccgccg
      121 atgtggcagg tgatgcccag caccctgccg ggctggatcg acctgttcgc catgggcatg
      181 gcgctggccg tggcgagcgc ctggacggcc gagcacggca gggagccggc ggtggcctcc
      241 gcccgctggg cgccatgggc ggcgtgggcc ctggcggccg ccgccttctg ggcggtgtcc
      301 acccagatcg gcctgccccg ctccccgcta tacgacgcca cgctgtcgca atacctggcc
      361 cgccaggccc tctacggggc ctttgccctg ttcctgctgc ttccaggcat cttcggcccc
      421 caggaccgcg ggctgatccg agccctgttg cagaaccggg ccgtgcaggc ggccgggctg
      481 atctcctacg gcatatacct gtggcacgag acgctgctga ccaagttctt cgtctggttc
      541 caccggagga acttccaggt cagcttcccc aagctcttcc tcgccatcct ggccgccacg
      601 ctggccacct cggcggtgac gtacctggcg gtggagcgtc ccttcctgcg gatgaaggac
      661 cgccgcctgc tccgtctgcg cgagcagcgt gcgctgtagg gtgtcttgtc cctcgctggc
      721 tgctggagac cggtgacctc actcgaccac gatcccgcgg ccgctgcccc gccgcccacc
      781 ggagcacccc tgcccggggc cgtgcccggg ctcgacctcg ccgagctggc cgcctccgcc
      841 ggcctccggc gcatccacat cctggcctgg cgggacctcg acgaccccga ggcgggcggc
      901 tccgagctcc acgcggcgcg catcgcggag cggtgggccg ccgccgggct cgaggtcacc
      961 atgcgctcct cccacgccga cggccacccc cattacaccc gccgagacgg ctatcaggtc
     1021 atccgcaagg ccggccgcta cggcgtgttc ccgcgcacgg ccctctccgg tctagtcggc
     1081 cgaacaggcg accgcgacgc catggtcgag atctggaacg gcatgccgtt cttctccccc
     1141 ctctggaccc gctgcccccg ggtcgtattc ctgcaccacg tccacgccga gatgtggcgc
     1201 atggttatcc accccccggc cctggccaag gcgggcgagg ccgtcgagct ccgcctcgcc
     1261 ccgcccgtct accgccgcag ccgcatcgtg accctctccg agtcctcccg ccaggagatc
     1321 atcgacctcc tccacctccc ggccgccaac atcgacgtcg tgccccccgg cgtcgagccc
     1381 cgcttcggcc cggcgcccga ccccaaacgg gcccgctccg aacggcccct cgtcgtggcc
     1441 gtcgggcgcc tggtgccggt caagcgcttc gacctgctca tcgccgccct gaccgagctc
     1501 aaggtccgcc accccgacct ggaggccgtc atcgtcggcg agggctacga gcgccccgcc
     1561 ctcgaggccc ggcgccgggc ggccggcgcc gaggattgga tccacctccc cggccacctc
     1621 gaggacgacg ctctcgtcga gctgtaccgc aaggcgtggg tgctggcctc cacgtcggcc
     1681 cgggagggat ggggcatgac catcaccgag gcggcggcgt gcgggacgcc cgcggtcgtg
     1741 actcgcatag ccggtcacct cgacgccgtg gaggagggcg gcagcggcct gctggtcgaa
     1801 gccggcgccg acccactcgc cggcgccctc gacgccgtgc tggccaaccc cctgctgcgg
     1861 gagaagctgt cccgcggcgc cgtcgaacgg gccgcccgcc tcacctggga ggcgaccgcc
     1921 cgggggacgc tcgccgccct cgtggccgaa gcccgccgcc gccggccggc cccgcccgca
     1981 gcgcccgccg cgcctgccgc cccgcccccc ggagggtgac cggacccgag cgacgccacc
     2041 gcgacctcgg cgccgggctg gcctggctca tcctggccat cccctacctg cccctcctgc
     2101 tcacccaccc gggcaaggtc ggcgccgaca ccaaggccta cctgtacctc gaccccaccc
     2161 gcctgctcaa ggaagctccc tacctgtggg atcccaaggt ggccatgggc acggtcaccc
     2221 atcagaacat cggctacctg ctgccgatgg ggccgtggtt cgccgccctc cacgcggctg
     2281 ccgtgcccat ctgggtggcc cagcggctgt ggctcggcac cctgctgctg ctggcgggcg
     2341 ccggcgtctc ctgcctgctc cagaccctcg gctgggaccg ccgccacacc atcctggccg
     2401 ccctggccta cgagctgtcc ccgtacctcc tgctctacga ggcccgcacc agcgccatcc
     2461 tgctgccctg ggccggcctg ccgtggatga tcgccctcgt catccgggcc gcccgggccg
     2521 accggcggcg ctggcgcgac ccggccctgt tcgccgtcgt ggtcgccctc gtcggcggga
     2581 ccaacgccac cagcctgctc ttcgccggcc tgggccccgc cctgtggctg ccgttcgcgg
     2641 tgtggtccca ccaggtgaag cccggccggg cggtggccac cgccctgcgc atcggcgtgc
     2701 tcagcctcgg cacctcgctg tggtggatcg ccgggctgat ggcccagggc acctacgggc
     2761 tcaacgtgct caagtacacc gagaccgtgc ccgccgtggc ccgcacctcc tatccctacg
     2821 agatgctgcg cggcctgggc aactggtact tctacggcct ggaccgggtg agctactggg
     2881 tcacccccgc ccggtacctg acccagacgc cgctcgtcat cgcggccagc ttcgccatcc
     2941 ccctccttgc attggcctcg gcggcggccg tgcgctggcg ggagcgcagc tacttcatgc
     3001 tcctggtggc cgccggcctc gtcatcggcg tcggcgtgca cccctaccac caccccaccc
     3061 cgctcggcgc cgccttcaag gccttcgccc agggctcgag cgccggcctg gccctgcgca
     3121 ccacggcccg ggcggtgccg ctgctggtgc tcggaacggc cgtgctcctc ggggccgggg
     3181 tgaaggcgct ccttgagaaa agccacggtc tgctccaccg gccgccgtgg cgccttgccg
     3241 tccccctggc ctgcgcggcc ctcgtggccg tggcccagcc ggcgctgtgg accggcggct
     3301 tcgtcgagag cagcctcgac caccccgaga ccgtccccgc ctactgggcc caggccgctg
     3361 ccgcgctcac cgatgccggc ggcgccacga gggccctcga cctcccgggc gccgatttct
     3421 cctactaccg ctggggagcg accgtggacc cggccgagcc cgggctgacc acccggcccc
     3481 tcgtcggccg cgagctgacc cctttcggct ccgccgcctc cgccgacctg gtgcgagcgc
     3541 tcgaccgccg cctccaggag ggcgtgctcg agcccggcgc catcgccccc gtcgcccgcc
     3601 tcatgggcgc gggcacggtg ctggtccgca acgacctcca gtacgagcgg ttccgcacgc
     3661 cgcgcccccg caacgtcgag gagctgctga ccccgacccc gcccgggctg gacgcgccca
     3721 ccggcttcgg cccgcccgcg tcgatcccgc cgcgggtcac caccaacgac gaggtcaccc
     3781 tcggggtccc gccggccacg ccgtacccgc cctcggtcga gaccttcgcc gtgcaggacc
     3841 cggcgccgat cgtgcggacc gagccggcgg cgtcgccgtt gctcgtcgcc ggcgacggcg
     3901 agggcctggt cgaggccgct gccgccggcc tgctgacggg ccccggcccc gtcgtgtact
     3961 cgggctcggc ggcgtcggac ccctcgctcg tgcagggcgc cctcgccagc ggggccgacc
     4021 tgctgctcac cgacaccaac cgccggcggg cgcaacggtg gggcacggtc cgggagaact
     4081 acggctacac cgagcggccc ggcgaggtcc cgctcgtgag cgacccgagc gacgcccgcc
     4141 tgccgctgtt ccccggcgcc accgactcgg ccttcacgac cgccgagcag cggggcgtct
     4201 cctctgtcat ggccacggcc tacggcgacc cggtggccta caccgccgcc gaccgcccgg
     4261 ccatggccct cgacagcgac ccggccacgg cctggaccgt ggccgccttc gacgaccccc
     4321 ggggccagaa gctggtcgtg accctggccc accccgtgac cgccgaccac ctcaccctgc
     4381 tccagccggt actgccgccc aacgagcgct acatcaccca ggcgcatctg atcttcgacg
     4441 gcgggccggc cgtgccgatc acgctcgacc cggtggcgtc gcggcgcgtg cccggcgaga
     4501 ccatcccgtt cccccgagcc accttctccc agctcacctt cgtcatcgac cagaccaact
     4561 acggcaaccg ggcagacttc tccggcgtca gcggggtggg cctggccgag ctgaccatcg
     4621 gcgacccgac attggcgcag cacgtggacg aggtcgtcca cctgccgacc gacctgctgg
     4681 gcctggccgg cccggcgagc gccggccaca acctcacggt tctgctcgcc cgggcccgga
     4741 gcaacccggc cgagccctac aagggcgacg aggagcaggc ggtcgtgcgt tcgttctcgc
     4801 tgccggcggg gcgggcgttc gcgctgagcg gcgaggccag gctggccagc gccgcgcccg
     4861 actccgtgtt ggacgcggtg cgaggccagc cgtctgtgtt ctcggcgtcg tcccggttgc
     4921 cgggcgatct cgacgcccgg gcggagatgg ccttcgacgg ctcaccttcc accgcctggc
     4981 agtcctcctt cggcgacgtc aacgcctggg tcgaggccga cgcccccgcc ccgatcaccg
     5041 tcgaccacgt ggacctcggg atcgtcgacg acggggtgca ctccgtgccc acccagctca
     5101 ccctgagcgt ggacggccgg cccgggccgg tgctggacgt gccgcgctcc ggcgccgttc
     5161 acctggcctt cccctccgtc aacgggtcgc gcttcaggct caccgtgtcg ggcctgcggg
     5221 aggtcacgac ggtcaactac ttctccaaga cgccccaggt caccccggtg agcctgaccg
     5281 aggtgggcct gggcgggctc cgggcgcccc cgcccccgcc gacgatcgac accggctgcc
     5341 gcccggacct gctgacggtg gacggccggc cctaccccgt gcgggtgacg ggcggcaccg
     5401 acgccgccga ccggggccag cccctcgccg tctcgccctg cggcccggcc ctccggctgg
     5461 gccccggcga ccacctggtg cgcgccgccg aggggcggct aacgggcctc aacctggacc
     5521 gcctggtgct gcgctccgcg gccgcggccg ctccggctcc cgccggggcg gctccgcctt
     5581 ctgcccccgc cgtgcacgtc acccacgacg gggccacgtc ggtgacggcg aaggtgaccg
     5641 gcgcggccgg cccgttctgg atggtgctgg gtcagagcca caacgccggt tggcacgcca
     5701 ccctcgccgg cacatcgctg ggcgcctcgc acctcgtgga cggctacgcc aacggctggc
     5761 tggtgcggcc gccccccggc gcggccgccc tcaccatcca catgacctgg acgccccagc
     5821 gccgggtctg gcaggcgctg gccgcgtccg ggatggtcct gctgttgtgc ctgctcctcg
     5881 gcttccgcag cggcggcacc gagaccgtca ccagctcgct gccggtgtgg cgctcgccgt
     5941 ggtcggccga gctgcccgcc ctcaaaggcc gcaccgtggt ggccgtggcc ggcgtgctgg
     6001 ccggcggggc cgtgctcgcc gggcccggga tcggcatcgc cctcgcggcg gtgaccgctg
     6061 tcgcggcggt gtggccccgc gcccgggtgc tgctgaccgg cggggcggtg gcgtgcgtgg
     6121 ccgtcatcgc ggcgtacgtc ctcgagctcc aggtccgcta cgcctttccc ctcaagctct
     6181 cgtggccggc ccacttccag ctcgtcgccc ccctgggctg ggccgccgtg ggcctgatcg
     6241 tggccgacgg ggtgctcgag tgggcccgcg agggctgaac cacgcca
//
LOCUS       DASWGG010000399         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_242228, whole genome shotgun sequence.
ACCESSION   DASWGG010000399 DASWGG010000000
VERSION     DASWGG010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242228"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2012)
                     /locus_tag="VF954_07355"
     CDS             complement(<1..>2012)
                     /locus_tag="VF954_07355"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9530937.1"
                     /translation="GNRGKRSIVVDLGHPAGRDRLDRLVERADVVLESPGGAGRRARL
                     VDVDAALRGNPRLVVTSISAFGLDGPYADLPGPDLIVAALSGLVWLTGDEDRAPLRVS
                     VPQSFRQASAEAAVHTLVALHHAARTGQGQHVDVAALPTMVRTIMNASEYEALEGVGI
                     TRMGSRASTVPNRPRVLFSCADGEVVFMTALGPMGGPAIQHLISEADDAGIEVPKALR
                     DVDLLDPRAWHAAEADGRIGGMAAALEAVISGVFALHTKEELYDAALEQGWLLAPVNT
                     VADLHADRQLAARDFWCKAETPDGATVAFPGPWARLAATPMREAAPAPPLGQDAQPGW
                     EHAASPPGPGGEAAGQDPFAGLKVWDMSWVGVGPLTSRYLADYGATVVRLDHVVRADV
                     LRVSPPFAGGVPGINRSQFYADFNSSKLGIGLDIARPEGQEVAQRLAAWADVVLESFT
                     PRTLRGLGLHYEDLRTVNPSLVMLSTCMQGQTGPCRDYRGFGQLMGALTGFYEVTGWP
                     DRGPTMVWGAYTDFVAQRFCATALIAAIDHRQRTGEGQHIDVSQLEASLQVLGPELLD
                     YAANDRVVTRQGNRDPSAAPHGIYPCRPIGPGPGGERWIAIVVEDDEQWEALVAALGR
                     PAWATAGTLGTVRGRKANEDEIEGRLAEWTAELEIDECLALLRPAV"
BASE COUNT          268 a          745 c          744 g          255 t
ORIGIN      
        1 cgaccgccgg ccgcagaagg gccaggcact cgtcgatctc cagctccgcg gtccattcgg
       61 ccagccggcc ctcgatctcg tcctcgttcg ccttccgccc ccgaacggtc cccagcgtgc
      121 ccgcggtcgc ccaggcgggc cggcccaggg ctgccaccag cgcctcccac tgctcgtcgt
      181 cctcgaccac gatggcgatc caccgctcac cgccgggccc cgggccgatg ggccggcacg
      241 ggtagatgcc gtgcggggcg gcggaggggt cgcggttgcc ttgccgcgtc acgacccggt
      301 cgttggccgc gtagtcgagc agctcgggcc cgagcacctg gagggacgcc tccagctggg
      361 agacgtcgat gtgctggccc tcacccgtgc gctggcgatg gtcgatggcg gcgatcagcg
      421 cggtggcgca gaaccgctgg gcgacgaagt ccgtgtacgc cccccagacc atggtcgggc
      481 cgcggtccgg ccagcccgtg acctcgtaga agccggtcag ggcacccatg agctgcccga
      541 agccgcggta gtcccggcac ggaccggtct ggccctgcat gcacgtcgac agcatgacca
      601 gcgacgggtt gaccgtgcgg aggtcctcgt agtgcaggcc gaggccccgc aacgtccggg
      661 gcgtgaagct ctccaggacg acgtcggccc acgcggccag gcgctgggcc acctcctggc
      721 cctcgggccg ggcgatgtcc aggccgatgc cgagcttgga ggagttgaag tcggcgtaga
      781 actgcgagcg gttgatgccg gggaccccgc cggcgaacgg cgggctgacc cgcagcacat
      841 cggcgcgcac cacgtggtcg aggcgcacca cggtggcgcc gtagtcggcc aggtagcgcg
      901 aggtcagggg gcccacgccc acccacgaca tgtcccacac cttcagcccc gcgaacgggt
      961 cctggccggc ggcctcgccg ccggggccgg gtggtgaggc ggcatgctcc cacccgggct
     1021 gggcgtcctg gcccagcggc ggggcgggcg cggcctcgcg catcggagtg gcggccaggc
     1081 gcgcccaggg gccgggaaag gcgaccgtcg cgccgtccgg cgtctcggcc ttgcaccaga
     1141 agtcgcgggc cgcgagctgc cggtcggcgt gcaggtcggc caccgtgttg accggcgcca
     1201 gcagccatcc ctgctccagc gcggcgtcgt agagctcctc cttggtgtgc agggcgaaca
     1261 cgccggagat gaccgcttcc agcgcggcgg ccatgccccc gatccggccg tcggcttccg
     1321 cggcatgcca ggcccgggga tctaggaggt cgacgtcgcg cagcgccttc gggacctcga
     1381 tgcccgcgtc gtcggcctca ctgatcaggt gttggatggc gggcccgccc atcgggccca
     1441 gcgcggtcat gaacacgacc tccccgtcgg cgcagctgaa cagcacccgg ggacggttgg
     1501 gtacggtcga cgcccgggag cccatgcgag tgatgccgac gccctcgagg gcctcgtact
     1561 cggaggcgtt catgatcgtg cggaccatgg tgggcagggc ggccacgtcc acgtgctggc
     1621 cctgcccggt gcgcgccgcg tggtgcagcg ccaccagggt gtgcaccgcg gcctcggcgg
     1681 acgcctgtcg gaaggactgg ggcaccgaga ctcggagcgg ggcgcggtcc tcgtcgccgg
     1741 tcaaccacac cagccccgac agggccgcga cgatcagatc gggcccgggg agatcggcgt
     1801 agggaccgtc cagcccgaag gccgagatcg acgtaaccac cagccgcggg ttgccgcgca
     1861 gggcggcgtc gacgtctacc agccgcgccc tgcgccccgc cccgccgggc gactccagca
     1921 ccacgtcggc ccgctcgacg aggcggtcga gccggtcccg gccggcgggg tgccccaggt
     1981 ccacgacgat ggagcgcttg ccccgattgc cg
//
LOCUS       DASWGG010000400         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_245511, whole genome shotgun sequence.
ACCESSION   DASWGG010000400 DASWGG010000000
VERSION     DASWGG010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245511"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1569)
                     /locus_tag="VF954_07360"
     CDS             complement(<1..>1569)
                     /locus_tag="VF954_07360"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX9530938.1"
                     /translation="PLRERLDAVLHVVPLGVPETASALVTLAGLGLVLLAWGIRRGQR
                     HAWTVSVGLLAWTVVLHIVKGADLEEAGLAAMVAAFLVRNRASFRAATDRPSQRRAVL
                     AVAFGGLGVVGLATGVVEVVTATRGRGGRIALGRAAESVTERMIGIRHVGLPHRLDLF
                     VTPSMEALAAGLAAIVAWLALRPVVARRHADADTGLAKAREIVAGYGQGTLDFFALRT
                     DKRYFFNGSSLVAYAVYGGVCLVSPDPIGPESERDQVWKAFREFADSHGWTLAVLGAS
                     EDWLPTYRASGMHDLYVGDEGVVDVGRFSLEGGKHKGLRQAVNRIAKYGYTISFHDPC
                     AMAAPAREQLRAVMTKSRQGEVERGFSMTLGRVFDPEDRGLLLAVAHDPSGEAVAFCQ
                     YVPANGIDGYSLDLMRRDEGPHPNGLIDFVVVRTIEHLRALGKRGLGLNFATMRAVLA
                     GEAGDSLTQRVERWLLKRMSGSMQIESLWRFNAKYDPAWQPRYAVYDSPEHMVTVAMA
                     IARAESFWELPLIGR"
BASE COUNT          235 a          575 c          558 g          201 t
ORIGIN      
        1 cgcccgatga ggggcagctc ccagaacgac tcggcccggg cgatggccat ggcgacggtg
       61 accatgtgct cgggcgagtc gtacacggcg taacggggct gccaggcggg gtcgtacttg
      121 gcgttgaacc gccacagcga ctcgatctgc atcgaccccg acatccgctt cagcagccac
      181 cgctcgaccc tctgggtgag cgagtcgccg gcctcgccgg cgagcacggc ccgcatggtg
      241 gcgaagttca ggcccaggcc gcgcttgccc agggcgcgga ggtgctcgat cgtccgcacg
      301 accacgaagt cgatgagccc gttcgggtgg gggccttcgt cgcgtcgcat gaggtcgagc
      361 gagtagccgt cgatgccgtt ggcgggcacg tactggcaga acgccaccgc ctcgcccgac
      421 gggtcgtggg ccaccgccag cagcagcccc cggtcctctg ggtcgaagac tcgccccaac
      481 gtcatggaga agccccgctc cacctccccc tggcggctct tggtcatcac ggcccgcagc
      541 tgctcccgcg cgggcgcggc catggcgcag ggatcgtgga aggagatggt gtagccgtac
      601 ttggcgatgc ggttgacggc ctggcgcagg cccttgtgct tcccgccctc gagcgagaag
      661 cgccccacgt cgaccacgcc ctcgtcgccc acgtacaggt cgtgcatgcc cgaggcccga
      721 taggtgggca gccagtcctc gctggctccc aggacggcca atgtccagcc gtggctgtcg
      781 gcgaactccc ggaacgcctt ccacacctgg tcccgctccg actccggccc gatggggtcg
      841 ggggagacga ggcagacccc gccgtacacg gcgtaggcca ccaggctcga cccgttgaag
      901 aagtaacgct tgtcggtgcg cagggcgaag aagtcgagcg ttccctgccc gtagccggcg
      961 acgatctcac gggctttggc caggcccgtg tcggcatcgg cgtggcggcg ggcgacgacc
     1021 ggacggagcg ccagccacgc cacgatggcg gccaggccgg cggccagagc ctccatggac
     1081 ggggtcacga agaggtccag ccggtgcggc aggccgacgt ggcggatgcc gatcatgcgc
     1141 tcggtcaccg actcggcggc gcgacccagc gcgatccggc cgcctcgccc gcgggtcgcg
     1201 gtcaccacct cgaccacgcc ggtcgccagt cccacgacgc ccaggccgcc gaaggcgacg
     1261 gccagcaccg cccgtcgctg cgacgggcgg tcggtggcgg cccggaacga cgcccggttg
     1321 cgcaccagga aggccgccac catggcggcc agcccggcct cttccaggtc ggcgcctttc
     1381 acgatgtgca gcacgacggt ccaggccagc aggccgaccg acacggtcca ggcgtggcgc
     1441 tggcctcgtc tgatgcccca cgccagcagg accaggccca gcccggccag ggtgaccagg
     1501 gcgctggccg tctcgggcac gccgaggggc accacgtgga gcacggcgtc gagccgctcg
     1561 cgcaggggc
//
LOCUS       DASWGG010000401         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2451344, whole genome shotgun sequence.
ACCESSION   DASWGG010000401 DASWGG010000000
VERSION     DASWGG010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2451344"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /gene="leuC"
                     /locus_tag="VF954_07365"
     CDS             complement(<1..1267)
                     /gene="leuC"
                     /locus_tag="VF954_07365"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX9530939.1"
                     /translation="MATKTTPQTLSDKKTLSDKVWDRHVVHAAADEPDLLYIDLHLVH
                     EVTSPQAFDGLRLAGRGVRRPDLTVATMDHNVPTTALDVSVADPVSRRQMEVLSANCA
                     EFGIPLYPFGDRNQGIVHVIGPQLGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEV
                     EHVLATQTLPQARPGTMAVTVDGDAPAGVTAKDIILAVIGRIGTGGGLGSVVEYRGSA
                     IRALSMEGRMTVCNMSIEAGARAGLVAPDDVTFAYLEGRSHVPQGGAWERALDEWRAL
                     PTDDGAAFDKEVTLDAGNLRPHVTWGTNPAQVVPIDGAVPDPDSFAEAAERQAAARAL
                     QYMGLTAGTPMHDIRVDTVFIGSCTNSRIEDLRAAARIADGRTVAPGVRALVVPGSMQ
                     VKAQAEQEGLDRVFTAAGFEWREAGCSMCL"
     gene            1303..1938
                     /locus_tag="VF954_07370"
     CDS             1303..1938
                     /locus_tag="VF954_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX9530940.1"
                     /translation="MEQSVSGVGVLDKAVSILAALEAGPCSLAGVVEATGLTRATAHR
                     LAGALEVHGFVGRDTQGRYAVGPRVAGLGLAAAARPALEVLRDATGESVQLFVRRGAQ
                     RICILALESPHGLRTIVPVGAVLPLERGSAGAVLRDDLQAGRRGWAQSVGEREAGVAS
                     VSAPVRRLGRTVAAVSVSGPIERTTRSPGKRFATDVVRAARSVEAAMGWAP"
     gene            1941..>2624
                     /locus_tag="VF954_07375"
     CDS             1941..>2624
                     /locus_tag="VF954_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX9530941.1"
                     /translation="MALSERDRRIVALAVPALGTLAVEPLYVLVDTAIVGRLGTTPLG
                     GLALATTVLTTLLWVFNFLSYGTTARVAFLMGRGQRERSGDVAAQGLWLAAGLGIPVA
                     VVVGAGGRLLPAALGGHGAVLATAATYLRISAFGIPAVLVALVGQGYLRGVLDTRSPL
                     RVVVAANLLNVVLELVLVYGAHLGIAGSAWGTVAAQWVAAVWFLVLVGARLGAVGAVR
                     RPVLAEIRRL"
BASE COUNT          289 a          945 c          987 g          403 t
ORIGIN      
        1 ccaggcacat cgagcagcct gcctcccgcc actcgaagcc ggcggccgtg aagacccggt
       61 cgagcccctc ctgctcggcc tgggccttga cctgcatcga gccgggcacc accagggcgc
      121 gcacgccggg tgccaccgtg cgaccgtctg cgatccgggc ggcggcgcgc aggtcttcga
      181 tgcgggagtt ggtgcagctc ccgatgaaca cggtgtccac gcggatgtcg tgcatcgggg
      241 tgccggcggt caggcccatg tattgcaggg cccgggccgc ggcctggcgc tcggccgcct
      301 cggcgaagct gtccggatcg ggcacggccc cgtcgatggg gacgacctgg gccgggttgg
      361 tgccccacgt gacgtgcggt cgcaggttcc cggcgtcgag ggtgacctcc ttgtcgaaag
      421 ccgcaccgtc gtccgtgggc agggcgcgcc actcgtccag ggcgcgctcc caggcaccgc
      481 cctggggcac gtgggaccgg ccctcgaggt aggcgaaggt gacgtcgtcc ggggccacca
      541 gccccgcccg ggcgccggct tcgatggaca tgttgcagac cgtcatgcgg ccttccatgg
      601 atagggcccg gatggccgag ccccggtact cgacgaccga gcccagaccc ccgcccgtgc
      661 cgatgcgccc gattacggcc aggatgatgt ccttggctgt cacgcccgca ggcgcgtcgc
      721 cgtccacggt cacggccatg gtgccgggcc gggcttgcgg cagcgtctgg gtggccagga
      781 cgtgctccac ctcgctggtg ccgatcccga acgccagcgc cccgaaggcg ccatgggtgg
      841 aggtgtggct gtcgccgcag acgatcgtca ttcccggctg ggtcaggccg agctggggcc
      901 cgatcacatg cacgatgccc tgattgcggt cgccgaacgg gtacagcggg atgccgaact
      961 cggcgcagtt ggccgagagc acctccatct gccggcgcga tacggggtcg gcgacggaga
     1021 cgtcgagcgc ggtggtcggc acgttgtggt ccatggtcgc caccgtgagg tccggccggc
     1081 ggaccccccg gccggccagt cgcagcccgt cgaaggcctg gggcgaggtg acctcgtgca
     1141 ccaggtggag gtcgatgtag agcaggtcgg gctcgtcggc ggcggcgtgg accacgtgcc
     1201 ggtcccacac cttgtcgctc agagtcttct tgtcgctcag agtctgcggt gttgtcttcg
     1261 tcgccatggc cgtctcacat tctgggatgc tattctcgct ctgtggaaca gagtgtaagc
     1321 ggcgtcggcg tgctcgacaa ggcggtctcc atcttggccg ccctcgaggc cgggccctgc
     1381 tccctcgccg gagtggtgga ggcgaccggt ctcacccggg ccaccgccca ccgcctggcc
     1441 ggcgcgctcg aggtccatgg cttcgtcggg cgcgacacgc agggccggta cgccgtcggc
     1501 ccccgggtgg ccgggctcgg attggcggcc gccgcccgcc cggcgctcga ggtcctgcgg
     1561 gacgccacgg gggagagcgt gcagctcttc gtccggcggg gcgcccagcg catctgcata
     1621 ctggccctcg agtcgcccca tggcctgcgc accatcgtcc cggtcggggc cgtgctcccg
     1681 ctcgagcggg gctcggcggg cgccgtgctg cgcgacgact tgcaagcggg ccggcggggg
     1741 tgggcccaga gcgtgggcga gcgggaggcc ggggtggcct cggtgagcgc gccggtgcgc
     1801 cgtctcggtc gcaccgtggc ggcggtgtcg gtgtccggcc ccatcgagcg caccacccga
     1861 tcgccgggca agcggttcgc caccgacgtg gtgcgcgccg cccggtcggt cgaggccgcc
     1921 atgggctggg ccccgtaggg atggccctct cggagcggga ccggcgcatc gtggccctcg
     1981 cggtgccggc cctgggcacc ctcgccgtcg agccgctcta cgtcctcgtc gacaccgcca
     2041 tcgtcggccg gctcgggacc accccgctcg ggggcctggc gctggccacc accgtgttga
     2101 cgaccctgct gtgggtcttc aacttcctgt cctacggaac gaccgcgcgc gtggccttcc
     2161 tcatgggtcg gggccagcgc gagcgctcgg gagacgtggc ggcccagggg ctgtggctgg
     2221 cggccgggct cgggatcccg gtggcggtgg tggtgggcgc gggcggacgg ctgctcccgg
     2281 ccgccctcgg aggccatggc gccgtgctcg ccaccgcggc cacctacctg cggatcagcg
     2341 ccttcggcat ccccgccgtg ctcgtcgccc tcgtcggcca gggctacctg aggggcgtcc
     2401 tcgacacccg cagcccccta cgggtggtgg tcgccgccaa cctcctcaac gtcgtgctcg
     2461 agctggtgct cgtctacggt gcccacctgg ggatcgccgg ctcggcgtgg gggacggtcg
     2521 ccgcccagtg ggtggccgcg gtctggttcc tggtgctggt cggagcgcgc ctcggcgcag
     2581 tcggagccgt gcgccgcccc gttctcgcgg agatccgccg gctc
//
LOCUS       DASWGG010000402         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2185058, whole genome shotgun sequence.
ACCESSION   DASWGG010000402 DASWGG010000000
VERSION     DASWGG010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2185058"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2157)
                     /locus_tag="VF954_07380"
     CDS             complement(<1..2157)
                     /locus_tag="VF954_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX9530942.1"
                     /translation="MRRGESLWSIAAAECASPLYWRDIADLNAVEHPALIHPGTVLRL
                     PAACEVPEHVAYVVAPGDTLSRIAQREYGIGGAWEAIWHANRGSVMSDGRTFTDPGLI
                     LPGWTLRLPTWPGSFWAPAAVATPAPEPAGGAPAPQEPAPAPTGTTAAPDTIASPETS
                     APAHPARPPGASPETLAPAHPARPPGASPADVPTNQITVGHGVRIPAGLLIAPCVASG
                     VLALALLARRRRRGARPLDSPEPRRADPPIIGGIRAAGTVASIDPLAPHTEALSSVLG
                     AAGHMPRVLGAWDDGERVRFVLDADAATLPAGTTHEGLGIRVEFSDVDGLAVATTTGE
                     RLPGLFRDPVAWTDDLLVPVGRHEPEGWLHLPLLSTPIAVTGPEAPELVSAVLLAAAV
                     RAGAGELRVFLAGEVRLDASPPEGMDLPAFETIPTDGLPALLAQEAHARASVVAAKSC
                     DNFAELSAAWPGLYPAWVLVLDAATAAACAGELATLADLGVGALVLGDLPEARTLRLS
                     GGSLDVHAPGLGTFGDLVPHLLPAAALVNELEAEALPLEPGPADEESLTPAAEPDAPV
                     RIHVLGGYRVQRHGAEVALTDTTSAQARELVAFLAVAGPTSAVRLREILWPEEHDAGD
                     VRRQSLHPLVSKARAWIGTPDAEDPVVHEGGIYRLGSQVWIDVAAFRSAVASGDPASL
                     KEAIGLYGELLDGQDSGRRFGWVKAEGYRDAERSRYL"
BASE COUNT          312 a          754 c          930 g          275 t
ORIGIN      
        1 gagatagcgg gagcgctcgg cgtcgcggta tccctcggct ttgacccagc caaatcgccg
       61 tccgctgtcc tggccgtcga gcagctcgcc gtagagaccg atggcctcct tcagggacgc
      121 cggatccccc gatgccacgg ccgagcggaa ggcggcgacg tcgatccaca cctgcgagcc
      181 gaggcggtag atcccgccct cgtggacgac ggggtcctcg gcgtcagggg tgccgatcca
      241 ggcccgggcc ttggaaacga gggggtgcag gctctggcgt cggacatcgc cggcgtcgtg
      301 ctcctcgggc cagaggatct cccgcaggcg caccgctgag gtcggcccgg cgacggcgag
      361 gaaggccacc agctcacggg cctgggccga ggtcgtgtcc gtcagggcga cctccgcgcc
      421 gtggcgctgg actcggtagc cgccgaggac gtggatccgc acgggggcat cgggctcggc
      481 ggcgggtgtc agggactcct cgtcggccgg cccgggctcc agggggaggg cctcggcctc
      541 cagctcgttt acgagggcgg cggcgggcag gaggtggggg acgaggtcgc cgaaggtgcc
      601 gaggccagga gcgtggacgt cgaggctgcc gcctgagagg cgcagcgtcc tggcctcggg
      661 gagatctccg aggaccaggg cgccgacgcc caggtcggcc agggtggcga gctcgcccgc
      721 acaggcagcg gcggtggcgg cgtcgagcac cagcacccat gcgggataca agcccggcca
      781 cgctgcgctg agctcggcga agttgtcgca cgacttggca gcgacgacgc ttgccctggc
      841 gtgcgcctcc tgggcaagga gggcgggcag accgtcggtg ggaatggtct cgaacgcggg
      901 gaggtccatg ccttcgggcg gtgaggcatc gagcctgact tccccggcga ggaagacccg
      961 cagctcgccg gcccccgcac gcacggcggc cgccagcagc accgcagaga caagctcggg
     1021 ggcctcggga ccggtcacgg cgatcggcgt cgagaggagg gggaggtgca gccagccctc
     1081 cggctcatgg cggcccacgg ggacgaggag gtcgtccgtc caggcaaccg ggtcgcggaa
     1141 cagcccgggc aggcgctcac ccgtcgtggt ggccacagcc aggccgtcga cgtcggagaa
     1201 ctcgactcgg atcccgaggc cctcgtgcgt ggtgccggca ggaagggtgg cggcgtcggc
     1261 gtcgagcacg aagcgtaccc tctcgccgtc gtcccacgcc ccgagcaccc ggggcatgtg
     1321 gccagcggcg ccgaggaccg acgagagggc ctcggtgtgc ggggccagcg ggtcgatcga
     1381 cgcgacggtg ccggcggccc ggatgccgcc gatgatcggt gggtccgccc ggcggggctc
     1441 gggggagtcg aggggccggg cgccacggcg tcggcggcgg gcgagcaggg cgagggcgag
     1501 gactccggag gcgacgcagg gggcgatcag caggccggcg gggatccgca cgccgtgccc
     1561 gaccgtgatc tggttggtcg gtacgtcggc cggggatgca ccgggcggcc gcgctgggtg
     1621 agccggcgcc aacgtctccg gggatgcacc gggcggccgc gctgggtgag ccggcgccga
     1681 cgtctccggg gatgcgatgg tgtcgggagc ggctgtagtg ccggtcggag cgggcgcagg
     1741 ctcctgggga gcgggggcgc cgccggccgg ctctggggcc ggggtcgcga ccgctgccgg
     1801 agcccaaaag gagccgggcc acgtgggcag tcgcagcgtc cacccgggca ggatcaggcc
     1861 ggggtcggtg aacgtgcggc catctgacat gaccgatccc ctgttggcgt gccagatggc
     1921 ctcccacgct ccgccaatgc cgtactcgcg ctgggctatc ctgctgaggg tgtcgcccgg
     1981 ggccacgacg taggccacgt gctccgggac ctcacaggct gctgggaggc gcagcaccgt
     2041 ccccggatgg atgagggcgg ggtgctcgac ggcgttgagg tctgcaatgt cccgccagta
     2101 cagggggctt gcgcactcgg cggcggcgat cgaccaaagg gactcaccgc ggcgcacaac
     2161 atgctcccgc tgagcggact gggcctctcc cgaaggggtg ccgggagagg ccgggggcgg
     2221 cgctccccca ccggccggat ccgggtgctc cggggcgctg acggaggtcg a
//
LOCUS       DASWGG010000403        11520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2185227, whole genome shotgun sequence.
ACCESSION   DASWGG010000403 DASWGG010000000
VERSION     DASWGG010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 11520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11520
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2185227"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..846
                     /locus_tag="VF954_07385"
     CDS             244..846
                     /locus_tag="VF954_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX9530943.1"
                     /translation="MIHAERRALATDLADIGPDQWAAPSLCSGWTVREVLAHMTATAK
                     ITPAKFLPGIVGSGFSFEKLQAKGVAAELGQTPSDTLSRFTEVAAATSKPPGPIDTAL
                     GETLVHSEDIRRVLGIKHEYDPAALEQVADFYRRSNLIIGGKKRAAELTFRATDADWT
                     AGSGPEVSGPMLSLTIAIAGRKAALDDLEGAGVETLRARA"
     gene            851..1735
                     /locus_tag="VF954_07390"
     CDS             851..1735
                     /locus_tag="VF954_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9530944.1"
                     /translation="MVEYDELGLFHENAAEWGLPYDGPPEVRRTAVTVTGDRAMSALV
                     WGSAPAELVLVHGGAQNAHTWDTVAMALHLPLVAVDLPGHGHSDGPRSESATAYDHAE
                     DVADAITELAPDARAVVGMSLGGLTSLALAQRHPQLVRRLALVDVTPGVTPSKGAAIT
                     AFINGPATFPSFDALLARTIEHNPTRSESSLRRGILHNAVQQPDGSWVWRWARFPRRE
                     RPGPELFATLWDAVSELTMPLMLVRGTRDGSVVDDDDVAELLRRQPAARVEPVDAGHS
                     VQGDAPLELAALIASFLE"
     gene            complement(1755..2732)
                     /locus_tag="VF954_07395"
     CDS             complement(1755..2732)
                     /locus_tag="VF954_07395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX9530945.1"
                     /translation="MRAVICKAFGPPESLVLEEVDEPRARPGQVVVDVGACAINFTDV
                     LVIQDLYQSKPPLPFVPGGEIAGVVSEVGEGVEGLAVGDRVLASTGVGGLAEKASVQA
                     GAAISVPPGVDLVHASSFLYAYGTSHHALKDRARIGAGETLLVLGAAGGVGLAAVELG
                     SVMGAIVIAAASSDEKLALCRDHGAAMTINYQQEDLKQRVRDLTGGTGVDVVYDAVGG
                     PYSEPALRSMAWDGRFLVIGFAAGDIPKVPLNLPLLKGCSIVGVFWGSFVVRERERNE
                     ANVAELMKWWQEGRLRPHVSSQHPLEHAAEAFRELADRKVKGKVVVTMN"
     gene            complement(2779..3750)
                     /locus_tag="VF954_07400"
     CDS             complement(2779..3750)
                     /locus_tag="VF954_07400"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX9530946.1"
                     /translation="MAAALLLAACSGSSRPPHAALPPAPSRSPSTGVAPTSTTVPTGP
                     TPCSPTRPQPAGLSTVTMSYQGQDRTYALYVPASYSGTTSVPVVFEFHGYSSNAFQQV
                     VYGDFRPLADRDGFVIVAPNGQGRDQHYNLTREPGLQDDVAFVGAVADRVESQLCVDR
                     RRIYATGMSDGGAMTSALACGAAGRFAAFGPVAAELYLPTCAAGRDVSIAAFHGTADP
                     IVPFKGGGVSCCGSPTLGSAPDAMAGWAGHDGCAQQFTDTRLSPQVIERQWSGCHPGT
                     EVRFYMIQGGGHTWPGSKIALGPLGLTTNEISATDTLWAFFQAHPLT"
     gene            complement(3774..4415)
                     /locus_tag="VF954_07405"
     CDS             complement(3774..4415)
                     /locus_tag="VF954_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX9530947.1"
                     /translation="MAFRGWPVEAIEFYEGLEADNSKTYWQANKAVYASAVQAPMQAL
                     LDELAEEFGEGRIYRPYRDVRFSADKTPYKTAMGASLAHGGYVQLSSHGLGVGCGYYM
                     MAPDQLMSYRQAVADDALGTDLAARVAVAEKRGLSVSAHETLKTVPRGFPKDHPRSGL
                     LRHKGLITWKDWPAAAWLGTTQAKKRVVEALRSAQPLVQWLDAHVGPSALPGR"
     gene            4456..5325
                     /locus_tag="VF954_07410"
     CDS             4456..5325
                     /locus_tag="VF954_07410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9530948.1"
                     /translation="MQVGAVFPQNEIGTDPGGVRAYAEAVESIGYRHILAYDHVLGAD
                     PAVHAGWSGPYDVHSDFHEIMVLLGYLAGVCSLELATGIVILPQRQTVLVAKQASAVD
                     VLTGGRLRLGVGLGWNPVEYEALGENFHDRGRRVEEQIELMRLLWTEESVSYEGRWHK
                     LTGAGLSPMPVQRPIPLWFGGGGSERALERIGRLGDGWMPQHRPGEELDRALGVVRAA
                     ARQAGRDPGALGVEGRIEGGHKDVDRIAHHARKWSEAGASHLSVSTMYAGLESVDGHI
                     EALRRTAEAVGLD"
     gene            complement(5322..6263)
                     /locus_tag="VF954_07415"
     CDS             complement(5322..6263)
                     /locus_tag="VF954_07415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX9530949.1"
                     /translation="MAATSTEGGARPRLALGPIGLWTGALDMVPASRSRELAAELEDL
                     GYDAVWIPEVAGRDSFVHLTLLLGATTRLVGATGIANIWSRDAVAMAGGIKALTEAFP
                     ERALVGLGVSHRNLVDGLRGHHYDKPLTTMSAYLDAMDRAPYTAERPGTPVRRVLAAL
                     GPKMLALSAEKADGAHPYFVNPEHTRRAREVLGTGPLLCPEQAVLLETDPAQARQIGR
                     GYTAVYLSQPNYVNNIRRLGFSDDDMAAGGTDAFVDAMVAWGTLEQVQARVREHFDAG
                     ADHVCIQPLGSGRRAVPDAQWRELAPALAELGRAVRN"
     gene            complement(6316..7062)
                     /locus_tag="VF954_07420"
     CDS             complement(6316..7062)
                     /locus_tag="VF954_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530950.1"
                     /translation="MEPTQADRDDQGGTRPALAWLIGTGEGGRHFDKFLGHEMAAVDF
                     RRVGDATGLTKWELAAQLRGGKQRVPPERLAEALREFVDGMAEGDYVFAGDGRGHVLV
                     GLVTGPYRWQQRPPLANFNHTRSIHWLGGFALDELPDPVRMPVLHNQGSIHRFADQPA
                     ALAWCRLAEEGQGAPVPRVRAGPAGRGRPPTRRSPSAESSSPTRTRASRPASPPAAER
                     RLCSVCFSSLPTAHFDQGSTACRSCAAMDR"
     gene            complement(7126..8082)
                     /locus_tag="VF954_07425"
     CDS             complement(7126..8082)
                     /locus_tag="VF954_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530951.1"
                     /translation="MSPILDSHHDFAHPVEGDTAWSESYYFNAYDPKTDCGFYTRTAI
                     RPNEGTHDAGMSVWVPGGGLAHLRSVREQRTMIDRDLAVGPLRYQLVEPLKQWRITAE
                     GEGTTGPLAMDVAFEALMPAIGADGQGRQGSGSSAETGKMLNKGHLEQAGRWSGWIEV
                     NGQRHTLGPEARGNRDKSWGPRRWGGPTMWRWFSVNIGDDTHLGGIRIGTAAGDLHRG
                     WAWKDGEHASIQEWQVTSELEDDGITHRVSHVVALDKLGRKHELRGDLLRVEPGKAGI
                     GPDKTIVNEGLARWTYEGRTGYGISEYLHQLDANSRPVVAIE"
     gene            complement(8134..9111)
                     /locus_tag="VF954_07430"
     CDS             complement(8134..9111)
                     /locus_tag="VF954_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9530952.1"
                     /translation="MRVLVLGGNRYIGRHLVEELAGRGHEVTVLNSHPSPLPEGVRRW
                     HGDRRVPGVLESVLDDHRHDFDVVYDNTSFQVTDLEPLAALFAGRIEQFVFTSSVAVY
                     KRSYLHPVHESSPRHDPADRDPRKAYGVGKVQCEDYLSALHAEHGFPATSLRVSHTIG
                     PYSPLVTREPIFFARLEQGRPILVPGEGFPFVHLVHVADVARLMASVAGNPRAVGQVY
                     NVGGSEITSILGCMRLMGRAAGAEPTIVHVPIDLARRLPRPLLHWGEALLGGTIYSVD
                     KARRDLAWEPQLGLEAGYRDSYAWFSREGRDLYEYDFTFDDEVLAQLQP"
     gene            complement(9208..9630)
                     /locus_tag="VF954_07435"
     CDS             complement(9208..9630)
                     /locus_tag="VF954_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530953.1"
                     /translation="MAIIGLLMAAAAAAFTADVAYESTGRLYFGAFNRNFSMATGWAA
                     IVAVLIGVGGTLGLGLLFAGMRRARRRHAVVAEHQRTAAELEDDRRRLAAELDRERAA
                     RLRAEQTAPAGDPRSQGQVSAPARSSFDQMPSGERTDA"
     gene            9854..10453
                     /locus_tag="VF954_07440"
     CDS             9854..10453
                     /locus_tag="VF954_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530954.1"
                     /translation="MPWREPIRADMDDGSAATIATRARRTNALAFGFAAAGFVVTMVI
                     VGLVVEHLLVGQACPGLDRMAVTRQPSPAAGEAATYCLPGALEAQRQHPELPMNACIT
                     FHGTGVRPSVVLPTDDRWAAPLGVLISPDDHTLTLIWSGARDDLPIIVEGKGVVRVGV
                     EPSGPLPATEPYHSASAPLAAPLGRRSVRLIRPRPGSCD"
     gene            10612..10965
                     /locus_tag="VF954_07445"
     CDS             10612..10965
                     /locus_tag="VF954_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9530955.1"
                     /translation="MAEANRRGREVIVPTLVCAEVARGRVRTRALEAAVARHDRARGE
                     RPPLRLVDTDFDLARQVGAILEATNSGSERVVDAHVVAVCVPGGGGLVVTSDPDDIAG
                     LASAVPAARIRTTRP"
     gene            11190..>11520
                     /locus_tag="VF954_07450"
     CDS             11190..>11520
                     /locus_tag="VF954_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G1 family glutamic endopeptidase"
                     /protein_id="HEX9530956.1"
                     /translation="MLVLAVPASGGATATVRSPGGVLIPAHVGQVLPTGNADTSSLNW
                     SGYAVVAQAGHPITAVTQNWIVPTVSTVPAGFSSTWAGIGGYSTGDLIQAGTESDTAQ
                     TPYAWYEI"
BASE COUNT         1631 a         4142 c         4089 g         1658 t
ORIGIN      
        1 acgacctccg ccgtcatggg atcgtcgagc ggctcgccga gcacctcggc cagggcgtcg
       61 accagcccgt cggcccgctc ggagcgatgg aggtgcagca tcgtccgcta ttcccctagg
      121 cctatccgca tatctcccac cttagggagg gcgtgtgaca cgactggcgc cggatggaca
      181 acaatggggc cgcggcacga cacgataggg ggctagctca tggcagcgac cgacgtatgg
      241 ccagtgatcc acgccgagcg gcgtgcgctc gccaccgatc tcgcggacat cgggcccgac
      301 cagtgggcgg cgccatcgct ctgctctggg tggacggtcc gggaggtgct cgcccacatg
      361 acggcgacgg ccaagatcac gccggccaag ttcctgcccg gcatcgtcgg cagcgggttc
      421 agcttcgaga agttgcaagc caagggagtc gcggcggagc tgggccagac cccatcggac
      481 acgctgtccc gtttcaccga ggtggccgcc gccaccagca agccgcccgg gccgatcgac
      541 acggcgctgg gcgagaccct ggtgcacagc gaggacatcc gccgggtcct cggcatcaag
      601 cacgagtacg acccggcggc gctggagcag gtcgccgact tctaccggcg gtccaacctg
      661 atcatcggcg gcaagaagcg ggccgccgag ctcaccttca gggcgaccga cgcggactgg
      721 accgcgggca gcggccccga ggtcagcggc ccgatgctct cgctcacgat cgccatcgcc
      781 ggtcgcaagg ccgccctcga cgacctcgaa ggcgccggcg tggagacgct gcgtgcccgg
      841 gcctgagccg gtggtcgaat acgacgagct cggcctgttc cacgagaacg cggccgaatg
      901 gggactgccc tacgacggcc cacccgaggt gcgccgcacc gcagtgaccg tcaccgggga
      961 ccgcgccatg agcgcactgg tgtggggttc ggcgccggcc gagctggtgc tcgtccacgg
     1021 cggcgcccag aacgcccaca cgtgggacac ggtcgccatg gcccttcatc tcccgctcgt
     1081 agcggtcgac cttcccggcc acggccattc ggatgggccc cggtcggaga gcgccacggc
     1141 gtacgaccat gccgaggacg tggccgacgc catcaccgag ctggcgcccg acgcgcgcgc
     1201 cgtcgtcggg atgtcgctgg ggggcctgac gtcgctcgcc ctggcccagc gccaccccca
     1261 gctggtccgc cggctcgcgc tggtcgacgt cacgcccggc gtgacgccgt cgaagggcgc
     1321 cgccatcacc gccttcatca acggtcccgc caccttcccg agcttcgacg ccctgctggc
     1381 ccggacgatc gagcacaacc cgacccgatc agagagctcg ttgcggcgcg gcatcctcca
     1441 caacgccgtg cagcagcccg atggctcttg ggtgtggcgg tgggcccggt tcccgcgccg
     1501 cgagcggccc gggcccgagc tgttcgccac gctgtgggat gccgtctccg agctgaccat
     1561 gcccctcatg ctcgtgaggg gcacccggga cgggtcggtc gtcgacgacg acgacgtggc
     1621 cgagctgctc cggcgccaac ccgcggcgcg tgtcgagccg gtcgacgccg gccacagcgt
     1681 ccagggcgac gccccgctcg agctggccgc gctgatcgcc tccttcctgg agtgaccgtc
     1741 agcgccggtc aagctcaatt catggtgacg acgaccttgc ccttcacctt gcgatcggcg
     1801 agctcgcgga aggcctcggc cgcatgctcg agcgggtgct gcgacgagac gtggggcctc
     1861 agcctgccct cctgccacca cttcatcagc tcggccacgt tggcctcgtt gcgctcacgc
     1921 tcccgcacca cgaacgagcc ccagaacacc cccacgatcg agcagccctt gagcagcggg
     1981 aggttgaggg gcaccttcgg tatgtccccg gccgcgaagc cgatgaccag gaaccggccg
     2041 tcccacgcca tcgaccggag cgcgggctcg ctgtagggcc ccccgacggc gtcgtagacg
     2101 acgtccacgc cggtgccgcc cgtcagatcg cggactcgct gcttgaggtc ctcttgctgg
     2161 tagttgatgg tcatggccgc gccgtggtcg cggcacaggg ccaacttttc atccgacgaa
     2221 gccgccgcga tcacgatggc gcccatcacc gagccgagct cgacggccgc cagccccacc
     2281 ccgccggccg ccccgagcac cagcagggtc tcgcccgccc cgatccgggc ccggtccttg
     2341 agggcgtggt gcgacgtccc gtaggcgtac aggaagctcg acgcgtgcac caggtcgacc
     2401 cccgggggca cggagatggc cgcgccagcc tggaccgacg ccttctccgc caggcccccc
     2461 acgccggtcg aggcgagcac ccggtcgccc accgccagcc cctcgacgcc ctcgcccacc
     2521 tcgctcacga cgcccgcgat ctcgcctccg ggcacgaacg gcaacggagg cttggactgg
     2581 tacaggtcct ggatcaccag cacgtcggtg aagttgatgg cgcacgcccc gacgtcgacg
     2641 actacctgcc cgggccgcgc ccggggctcg tcgacctcct cgagcaccag cgattccggc
     2701 gggccgaagg ctttgcagat gaccgctctc acaagggccg cagcgtaacc gatgccgccg
     2761 cccgccggca tgctcgggct aggtcaacgg gtgggcctgg aagaaggccc acagcgtgtc
     2821 ggtcgcgctg atctcgttgg tggtgaggcc gagcgggccg agggcgatct tgctgccggg
     2881 ccaggtgtgg ccgccgccct ggatcatgta gaagcggacc tcggtgcccg ggtgacagcc
     2941 agaccactgg cgctcgatca cctgcgggga caggcgcgtg tcggtgaact gctgggcaca
     3001 gccgtcgtgg ccggcccagc cggccatggc gtcgggcgcc gagcccaggg tgggagagcc
     3061 gcagcaggac acccccccgc ccttgaacgg gacgatcggg tcggccgtgc cgtggaacgc
     3121 ggcgatcgac acgtcccggc cggcggcgca tgtcggcagg tagagctcgg cggccacggg
     3181 gccgaaggcg gcgaagcggc cggccgcccc gcacgccagc gcggaggtca tggccccccc
     3241 gtccgacatc ccggtggcgt agatgcggcg ccggtcgacg cagagctgcg actcgacgcg
     3301 gtcggccacc gcccccacga acgccacgtc gtcctgcagc ccgggctcgc gggtgaggtt
     3361 gtagtgctgg tcgcggccct ggccgttggg cgccacgatg acgaagccgt cccgatccgc
     3421 cagcggccgg aagtcgccgt acacgacctg ctggaaggcg ttggagctgt agccgtggaa
     3481 ctcgaagacc acggggacgc tggtcgtgcc ggagtacgag gccggcacgt acagggcata
     3541 ggtgcggtcc tggccctgat aggacatggt gacggtggac aggccggcgg gctgcggcct
     3601 ggtcggtgag cagggggtgg ggccggtggg cacggtggtc gacgtcggcg ccaccccggt
     3661 cgacggcgac cgcgatggcg ccggcgggag cgcggcgtgg ggcggccggc tgctccccga
     3721 gcacgccgcc agcagcagcg cggccgccac ggcagcggcc ccaccccggg ccgtcaccgc
     3781 ccgggcaggg ccgaaggtcc gacgtgggcg tcgagccact gcaccagggg ctgggcggag
     3841 cgcagcgcct ccacgacccg cttcttggcc tgggtggtgc ccagccacgc cgccgccggc
     3901 cagtccttcc acgtgatgag ccccttgtgc cggagcagcc cggagcgggg gtggtccttg
     3961 gggaagcccc gtggcaccgt cttcagcgtc tcgtgggccg acaccgacag gccccgcttc
     4021 tccgcgacgg ccacccgggc ggccaggtcg gtccccaagg cgtcgtcggc gaccgcctgg
     4081 cgatagctca tgagctggtc gggcgccatc atgtagtagc cgcagccgac gccgaggccg
     4141 tgcgaggaca gctggacgta gcccccatgg gcgaggctgg ctcccatcgc cgtcttgtac
     4201 ggggtcttgt cggcgctgaa gcgcacgtcg cggtagggcc ggtagatcct gccctccccg
     4261 aactcctcgg ccagctcgtc gagcagggcc tgcatcggtg cctgcaccgc cgacgcgtag
     4321 accgccttgt tggcctgcca gtacgtcttg gagttgtccg cctccagccc ctcgtagaac
     4381 tcgatggcct ccacgggcca gccccgaaac gccacgaacc gagcctaggg cctgctgccg
     4441 tgctatgacc gggccatgca ggttggcgcc gtcttccccc agaacgagat cggcaccgac
     4501 cccggcggcg tgcgggccta tgccgaggcg gtcgagtcca tcggctaccg ccacatcctg
     4561 gcgtacgacc acgtcctggg cgccgacccg gcggtccatg ccggctggag cgggccctac
     4621 gacgtgcact ccgacttcca cgagatcatg gtgctgctgg gctacctcgc cggggtgtgc
     4681 tcgctcgagc tggcgaccgg catcgtgatc ctgccccagc gccagacggt gctcgtggcc
     4741 aagcaggcct ccgccgtcga cgtgctgacg ggcgggaggc tgcggctggg ggtcggcctg
     4801 gggtggaacc cggtcgagta cgaggcgttg ggggaaaatt tccacgatcg cggccgacgc
     4861 gtcgaggagc agatcgagct catgcggctg ctgtggaccg aggagtcggt gtcctacgag
     4921 ggacgctggc acaagctcac cggcgccggc ctcagcccga tgccggtgca gcggccgata
     4981 ccgctgtggt tcggcggagg cggcagcgag cgcgccctcg agcgcatcgg ccggctcggc
     5041 gacggctgga tgccccagca ccggccgggg gaggagctcg accgggccct gggcgtcgtg
     5101 cgggccgcgg cgcgccaggc cggccgtgat cccggcgcgc tgggtgtcga gggccgcatc
     5161 gaagggggcc acaaggacgt cgatcgcatc gcccaccacg cccggaagtg gtccgaggcc
     5221 ggggcgtcgc acctgtcggt cagcaccatg tacgccgggc tggagtcggt cgacggccac
     5281 atcgaggcgc tccgccgcac cgccgaggcc gtcggcctgg actagttgcg cacggcccgg
     5341 cccagctcgg cgagcgccgg cgccagctcg cgccactgcg catcgggcac ggcccggcgc
     5401 ccgctgccca ggggctggat gcacacgtgg tcggcgccgg cgtcgaaatg ctcgcgcacg
     5461 cgcgcctgga cctgctcgag ggtgccccat gccaccatgg cgtcgacgaa ggcgtccgtg
     5521 ccgcccgcgg ccatgtcgtc gtcggagaag ccgagccgtc tgatgttgtt cacgtagttg
     5581 ggctgtgaga ggtagacggc ggtgtagccc cggccgatct ggcgggcctg tgccgggtcc
     5641 gtctcgagca gcaccgcctg ctcggggcac agcagcggtc ccgtccccag cacctcccgg
     5701 gcccgccggg tgtgctcagg gttgacgaag tacggatggg cgccgtcggc cttctccgcc
     5761 gacagggcca gcatcttggg gccgagggcg gcgagcaccc gtcgcaccgg tgtccccgga
     5821 cgctcggcgg tgtacggcgc ccggtccatg gcgtcgaggt aggcgctcat cgtggtcagc
     5881 ggcttgtcgt agtggtgacc tcgcagaccg tcgaccaggt tgcgatgtga cacgcccagg
     5941 cccaccaggg cccgctccgg gaacgcctct gtcagcgcct tgatgccgcc tgccatggcg
     6001 acggcgtcgc ggctccagat gttggcgatg ccggtcgctc ccacgagccg cgtcgtggcg
     6061 ccgaggagca acgtcaggtg cacgaagctg tcccggcctg ccacctcggg gatccacacc
     6121 gcgtcgtagc ccaggtcctc cagctcggcc gccagctctc tgctccggga ggcgggcacc
     6181 atgtcgaggg cgccggtcca caacccgatc gggcccaggg cgagccgagg acgggcgccg
     6241 ccttcggtcg atgtcgcggc catggcccga acctagcggc atgggcgcgg catcgtctgg
     6301 gctcgccgtt cgcccctacc ggtccatggc cgcgcagctc ctgcacgccg tcgatccctg
     6361 atcgaaatgg gctgtcggca ggctggagaa gcacacgctg cacagccggc gctcggccgc
     6421 ggggggcgag gccgggcgcg acgcccgggt gcgcgtcggc gacgacgact cggccgaggg
     6481 ggaccggcgc gtgggcgggc gtcccctgcc ggccggcccg gcccggaccc ggggcaccgg
     6541 ggcgccctgg ccttcctcgg ctaggcggca ccaggccagg gcggccggct ggtcggcgaa
     6601 gcgatggatc gatccctggt tgtggagcac aggcatccgt acggggtcgg gcagctcgtc
     6661 gagggcgaac ccgccgagcc agtggatcga tcgggtgtgg ttgaagttcg ccagtggcgg
     6721 ccgctgctgc caccggtagg gaccggtcac caacccgacc aggacgtggc cgcgcccgtc
     6781 gcccgcgaac acgtagtcgc cctcggccat gccgtcgacg aactcgcgca gcgcctcggc
     6841 caggcgctcc ggcgggaccc gctgcttgcc gccccggagc tgggcggcca gctcccattt
     6901 ggtcagcccg gtggcatcgc cgacccgtcg gaagtcgacc gcggccatct cgtggccgag
     6961 gaacttgtcg aagtgccggc caccctcgcc cgtgccgatc aaccaggcca aagccggacg
     7021 ggtcccaccc tggtcgtcgc ggtcagcttg tgtcggctcc acctctccag tcatacaaca
     7081 gtgcgagagg cggtggggcc atggcggacg tctgcgaacc gcgacctact cgatggccac
     7141 gaccggccgg ctgttggcgt ccagctggtg gaggtactcg ctgatcccgt agccggtgcg
     7201 gccctcgtag gtccatcggg cgaggccctc gttgacgatc gtcttgtcgg gcccgatgcc
     7261 cgcctttccg ggctcgacgc gcagcaggtc gccgcgcagc tcgtgctttc gccccagctt
     7321 gtcgagggct acgacgtggc tgacgcggtg cgttatcccg tcgtcctcca gctcgctggt
     7381 cacctgccac tcctggatgg aggcgtgctc gccgtccttc cacgcccagc cccggtggag
     7441 gtctccggcg gcggtgccga tccggatgcc accgaggtgg gtgtcgtcgc cgatgttgac
     7501 ggagaaccag cgccacatcg tgggcccacc ccagcgccgc ggaccccatg acttgtcccg
     7561 gttgccgcgg gcctccggcc cgagggtgtg gcgctggccg ttgacctcga tccatcccga
     7621 ccagcggccg gcctgctcga ggtggccctt gttgagcatc ttgccggtct ccgcgctcga
     7681 ccccgagccc tgccggccct ggccgtcggc gccgatggcc ggcatcaggg cctcgaaggc
     7741 cacgtccatg gccaggggcc cggtcgtgcc ctcgccttcg gcggtgatcc tccactgctt
     7801 gagcggctcg acgagctggt agcggagcgg gccgacggcg aggtcccggt cgatcatcgt
     7861 gcgctgctcg cgcacggagc gcaggtgggc cagcccgccg ccgggtaccc aaaccgacat
     7921 cccggcgtcg tgggtgcctt cgttggggcg gatcgccgtg cgggtgtaga agccgcaatc
     7981 cgtcttcggg tcgtaggcgt tgaagtagta cgactccgac caggcggtgt cgccttccac
     8041 cgggtgggcg aagtcgtggt ggctgtcgag gatcgggctc atggccacca ctttgcccca
     8101 attcgggcgc cccgcgccca tccgtcgggc ccgtcagggc tggagctggg ccaggacctc
     8161 gtcgtcgaag gtgaagtcgt actcgtagag gtcccggccc tcccgggaga accaggcgta
     8221 ggagtctcgg tagccggcct cgagcccgag ctgcggctcc caggccaggt cgcgccgggc
     8281 cttgtccacc gagtagatcg tcccgcccag aagcgcctca ccccagtgga gcagcgggcg
     8341 gggtaggcgc cgggcgaggt cgatcggcac gtggacgatg gtgggctcgg ctcctgcggc
     8401 ccgccccatg aggcgcatgc agcccaggat gctcgttatc tcgcttccgc ccacgttgta
     8461 gacctgtccc acagcccgcg ggttgccggc cacggatgcc atcagccggg ccacgtcggc
     8521 gacgtggacc aggtggacga acgggaagcc ctcgccgggg accaggatcg gccgcccctg
     8581 ctctaggcgg gcgaagaaga tgggctccct cgtgacgagc gggctgtagg gaccgatggt
     8641 gtgcgacacc cgcaggctgg tggctgggaa cccgtgctcg gcgtgcagcg ctgacaggta
     8701 gtcctcgcac tgaaccttgc ccaccccgta tgccttcctg ggatcacggt cggccggatc
     8761 gtgcctgggg gaggactcgt ggaccgggtg aaggtagctg cgcttgtaga ccgccaccga
     8821 gctggtgaac acgaactgct cgatccggcc ggcgaagagg gcggcgagcg gctccaggtc
     8881 cgtcacctgg aaggacgtgt tgtcgtacac cacgtcgaag tcgtggcgat gatcgtcgag
     8941 cacggactcg agcacgcccg ggacccgccg gtcgccgtgc cagcgccgca caccctccgg
     9001 caagggcgag gggtggctgt tgaggaccgt cacctcgtgg ccgcggccgg ccagctcctc
     9061 caccaggtgc cgaccgatgt agcggttgcc cccgagcacc aagaccctca cagccgtccc
     9121 cttccccgcg tggcgatgac gtcgcatcgt acgggttggg ggaagctcgc cggagctcgc
     9181 cgtggaagga gcgagcccac ggtgggctca ggcgtccgtg cgctctcccg aaggcatctg
     9241 gtcgaagctg gaccgggccg gggccgacac ctgtccctgc gagcgggggt cgcctgccgg
     9301 cgccgtctgc tcggcccgca ggcgagccgc tcgctcccgg tcgagctcgg ccgccaggcg
     9361 cctgcggtcg tcctccagct cggcggcggt ccgctggtgc tcggccacga ccgcgtggcg
     9421 ccggcgggcc cggcgcatgc cggcgaagag cagacccaga ccgagcgtgc cccccacgcc
     9481 gatcagcacg gccacgatcg cggcccatcc ggtggccatg ctgaaattgc ggttgaaggc
     9541 gccgaagtac agcctgccgg tcgattcgta ggccacgtcc gccgtgaagg ccgccgccgc
     9601 tgcggccatg agcagcccga tgatggccat cttgtccacc tccgaagtag acgttcctaa
     9661 aatgccgtac cccgtagcgc cccacccgac acccgttaac gccaggttgc ggtcccttgg
     9721 cgagttcccg gatcgaggtg ccggggcgac atgaagggct gctgaccgac caagctcgcc
     9781 aaatccgaca tgccgagttg ccgtggccga caattccgag gccgtggatc gggacccgcg
     9841 taccctcgcg atcgtgccgt ggcgtgaacc cattcgggcg gacatggacg acggctccgc
     9901 cgcgacgatc gcaactcggg ctcgtcgaac caacgccttg gccttcggat tcgcagccgc
     9961 ggggttcgtg gtgaccatgg tcatcgtcgg gctggtcgtg gagcacctcc tcgtcgggca
    10021 agcatgcccc ggcctcgacc ggatggcggt gacccgccaa ccgtcgccag cggccggcga
    10081 ggcggcgacc tactgcctac ccggtgctct ggaggcccaa cgccaacatc ccgagctccc
    10141 gatgaacgcg tgcatcacct ttcacggcac gggtgtgcga ccgagcgtgg tcctgccgac
    10201 cgacgaccgc tgggcggctc cgctcggcgt gctcatcagc ccggacgacc acacgctcac
    10261 cctgatctgg agcggcgccc gcgacgacct cccgatcatc gtcgaaggga agggcgtcgt
    10321 tcgggtcggc gtcgagccca gtggaccact gcctgcgaca gagccatacc actcggcgtc
    10381 agcccctctg gccgcgccgc ttggccgacg gtcggtgcgt ctgatccggc ctcgaccggg
    10441 ctcctgcgac taggtggcca accggtgccg attgtgtgga caactcccac aagccgaccg
    10501 agccgtggat caaggcattt gccggcaacc atgtccccgg cggttgcggc cgacaaggca
    10561 agctgtaggg catgtttcgt cggctcgctg cggcgcttgg cgccgtgacg actggccgag
    10621 gccaaccggc gtgggcgcga ggtcatcgtc cccacgctcg tgtgcgcgga ggtcgctcgc
    10681 ggccgcgttc ggacgagggc cctggaagcc gccgtggctc gccacgaccg agcccgcggg
    10741 gaacggccgc cgctgcgcct tgtcgatacc gacttcgatc ttgcccgcca ggtcggtgcg
    10801 atcctcgaag cgacaaacag cgggagcgag cgggtcgtgg acgctcacgt cgtcgccgtg
    10861 tgcgtacccg gtggaggcgg tctcgtcgtc accagcgacc cggacgacat cgcgggcctg
    10921 gcgtctgccg ttcccgccgc gcgtatccgc acgacacgcc cgtgacggcc cgcgggctgc
    10981 acacggatcc tggggcggtt caatgtccgc ctcccgtggg tctgaccgcc ggcattggca
    11041 gcccgagcat cacgggcacg cggagtcccg caagaatttg agcggtcgtc cgttacccga
    11101 acggcactgg gccgcaacgg gtcccggcgc gaccaggagg ggagcagatg accggaaggt
    11161 tgctcaggct cggggggctg gcgttcacca tgctggtgct ggcagtaccg gcaagcggag
    11221 gtgcaaccgc gaccgtccgc agcccgggcg gagtgctgat ccccgctcac gtcggtcagg
    11281 tcctgcccac cgggaacgcc gacacctcga gcctcaactg gtcgggctac gcggtcgtgg
    11341 cccaggccgg tcacccgatc accgcggtga cgcagaactg gatcgtcccg acggtgtcga
    11401 cggtaccggc cgggttctcg tcgacctggg ctggcatcgg tggctacagc actggagacc
    11461 tgatccaggc tggcacagag tccgacaccg cccagacgcc ctatgcctgg tacgagatct
//
LOCUS       DASWGG010000404         3720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_249154, whole genome shotgun sequence.
ACCESSION   DASWGG010000404 DASWGG010000000
VERSION     DASWGG010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249154"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VF954_07455"
     CDS             <1..498
                     /locus_tag="VF954_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530957.1"
                     /translation="RSLDVAPEGFDARRAATSRRYWYTVLNRPQNDPFLASTAWHVPK
                     PLGLRTMQLACDAFLGEHDFAAFCRKAPQAGGSTVRRVLDARWVDVGDGVLRFDVRAS
                     AFCHQMVRSIVGTHVDVGLGRITAGDMAWIIASRDRSHAGPPAPPQGLCLREVGYPPG
                     LAPHT"
     gene            complement(726..1922)
                     /gene="ilvA"
                     /locus_tag="VF954_07460"
     CDS             complement(726..1922)
                     /gene="ilvA"
                     /locus_tag="VF954_07460"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX9530958.1"
                     /translation="MITLREITEARGRLRPVIRPTPVVPSDALGRLTGRPVLLKPEHV
                     QRTGSFKVRGAYNFVSRLEAGTPVVAASAGNHAQGVALAAGLTGRRAVIFMPAAAPLP
                     KVEATRSYGAEVHLEGEAVDDCMRLAADHAAATGAVYVPPFDDPLVIAGQGTIGLELA
                     EEAPGMAVALVPVGGGGLIAGVATALAHTARSVRVVGVQAAGADAVVQSLRAQHRVTL
                     PRVGTMADGIAVSAPSELTLAHIRAYVDDVVTVTDEEISQAVLLLIERTKAVVEPAGA
                     VGVAALLAGKVAAPGPAVAVLSGGNVDPLLLTRLIDHGLSAAGRYLLLRIVLPDHPGA
                     LASLTGGVARMGLNVLSVEHRRAGARVGVDEVEVFLTLETRDPAHRESVAASLAEAGF
                     RVELLA"
     gene            2036..2485
                     /gene="rplM"
                     /locus_tag="VF954_07465"
     CDS             2036..2485
                     /gene="rplM"
                     /locus_tag="VF954_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX9530959.1"
                     /translation="MPTYSPKPADVQRAWHVVDADGLVLGRLATDVAVVLRGKHKAIF
                     APHLDTGDFVIVTNAAKVVLTSDKADKKLAYRHSGYPGGLRSRTYGDLLATRPEEVVR
                     RAVKGMLPKGPLGRHMLTKLKVYAGPTHPHTAQQPQPREIPGARRAS"
     gene            2514..2906
                     /gene="rpsI"
                     /locus_tag="VF954_07470"
     CDS             2514..2906
                     /gene="rpsI"
                     /locus_tag="VF954_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX9530960.1"
                     /translation="MPKPLTQTTGRRKRAVARVRLRPGGGKITVNRRLFEDYFPSATH
                     RMIVTEPLRLTSTEETYDVEVTMDGGGVSGQAGALRHGIARALVELDPELRISLKRAG
                     FLTRDDRKKESKKYGLKKARKAPQYSKR"
     gene            2915..>3720
                     /locus_tag="VF954_07475"
     CDS             2915..>3720
                     /locus_tag="VF954_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX9530961.1"
                     /translation="MRPSFGTDGVRGRANAELTPELVLALGRAAARRLGPGPMLVGRD
                     TRRSGPMLQAALTAGLASEGMDVVDLGVLPTPGVAFLSQRRDQPAAMISASHNPFADN
                     GVKFFLAGGRKLPVQVEAGLEAELAGILEDRDRDHDRDRGHPGAARPEGRGVGGAGPE
                     PGAAEEYTRHLVSTVPAGSGLGGLSVVIDCAHGAASALAAGVFRQAGAEVLVLSAQPD
                     GCNINDRCGSTDPGSLGRAVIEGGADLGLAFDGDADRVVAVDHQGTLVDG"
BASE COUNT          517 a         1434 c         1317 g          452 t
ORIGIN      
        1 cgttccctcg acgtggcccc cgagggcttc gacgcccggc gggcggccac gtcccgccga
       61 tactggtaca cggtgctgaa ccgcccccag aacgacccgt tcctcgcctc cacggcctgg
      121 cacgtgccca agccgctcgg cctgcggacc atgcagctgg cctgcgacgc cttcctcggt
      181 gagcacgact tcgccgcctt ctgccgcaag gccccccagg cgggcggctc gacggtgcgg
      241 cgcgtcctcg acgcccgctg ggtggatgtc ggcgacgggg tgctgcgctt cgacgtccgg
      301 gccagcgcct tctgccacca gatggtccgc tcgatagtcg gcacccacgt cgacgtcggc
      361 ctgggccgga tcaccgcggg cgacatggcc tggatcatcg ccagccgcga ccggtcccac
      421 gcaggccctc ccgcgcctcc ccagggcctg tgcctgcgcg aggtgggcta cccacccggc
      481 ctggcccccc acacctgagg cgtcgacgcc tctgcggagg cgatcgatcg ttcctcgcgc
      541 gcccggccaa actgccccgc ggcgacggcc agaagccgta gctggggaga tttggtggtc
      601 tcagaccgtc tcatctcccc agtaaaatga aatgacacga aatgctccat cggagccacc
      661 ctggccgaag gcaccgccgc cgccgccgcc gccgccaatg tcattcgagc ggacggaacc
      721 tcgggctagg cgagcagctc cacccggaac cccgcctcgg ccagcgacgc cgccacggac
      781 tcccggtggg cagggtcgcg ggtctcgagc gtgagaaaga cctcgacctc gtccaccccg
      841 acccgcgccc ccgcccgccg gtgttccacc gacaggacgt tgaggcccat gcgcgccacc
      901 ccgcccgtca gcgacgccag ggcaccggga tggtcgggca gcacgatgcg cagcaggagg
      961 tagcgcccgg cggcggacag gccgtggtcg atgagccggg tgagaagcag cgggtcgacg
     1021 ttgccgccgg agagcacggc cacggccggt cccggcgcgg cgaccttgcc ggcgagcagg
     1081 gcggccacgc ccaccgcgcc ggccggctcg accacggcct tggtccgctc gatcaggagg
     1141 agcacggcct ggctgatctc ctcgtcggtg accgtcacca cgtcatcgac ataggcccgg
     1201 atgtgggcca gggtgagctc ggacggggcg ctcaccgcga tgccgtcggc catggtgccg
     1261 acgcggggca gcgtcacccg gtgctgggcc cggagggact gcacgacggc gtcggcgccc
     1321 gcggcctgca ccccgacgac ccgcaccgag cgggccgtgt gggccagggc ggtggcgacg
     1381 ccggcgatga gcccgccgcc gccgacgggg accagcgcga ccgccatccc gggcgcctcc
     1441 tcggccagct cgagcccgat ggtcccctgg ccggcgatga ccagggggtc gtcgaagggc
     1501 ggcacgtaca ccgccccggt cgccgccgcg tggtcggcgg cgaggcgcat gcagtcgtcc
     1561 acggcctcgc cctcgaggtg gacctcagcg ccgtagctcc gggtggcctc caccttgggg
     1621 aggggagcgg ccgcgggcat gaagatcacc gccctgcggc ccgtgagccc ggccgccagg
     1681 gcgaccccct gggcgtggtt gccggcggag gcggccacaa cgggcgtgcc cgcctccagg
     1741 cgggacacga agttgtacgc cccccgtacc ttgaaggacc cggtgcgctg cacgtgctct
     1801 ggcttcagga gcacgggccg gccggtcagc cggcccaacg cgtcggacgg aactaccggg
     1861 gtcggccgga tcaccgggcg cagccggccc cgggcctcgg ttatttcccg gagggtgatc
     1921 acggcctcac cttaagacct ggtcggccgc cacttgccgg gcaccggccc ggcccgtatc
     1981 ctggctgcct gctctggcgc gcccggcgcg ccccgcctca tcgccgaagg gccctgtgcc
     2041 gacgtactcc cccaaaccag ccgatgtcca gcgcgcctgg catgtcgtgg acgccgacgg
     2101 gctcgtgctc ggccggttgg cgacggacgt tgccgtggtg ctccggggca agcacaaggc
     2161 catcttcgcc ccccacctcg acaccggcga cttcgtcatc gtgaccaacg cggccaaggt
     2221 cgtgctcacg agcgacaagg cggacaagaa gctggcctac cggcactccg gctacccggg
     2281 cggcctgcgg tcgcgtacct acggcgacct gctcgccacc cggcccgaag aggtcgtccg
     2341 gcgggcggtc aagggcatgc tgccgaaagg cccgctcggt cgccacatgc tcaccaagct
     2401 caaggtctac gccggtccga cccacccgca caccgcccag cagccccagc cccgggagat
     2461 ccccggcgcc cgccgggcgt cgtagcaccc cgacgatccc agagagcttc ccgatgccca
     2521 agcccctgac ccagaccacc gggcgccgca agcgcgccgt cgcccgcgtc cggctccgcc
     2581 ccggcggcgg caagatcacc gtcaaccgcc gcctgttcga ggactacttc ccctcggcga
     2641 cccatcgcat gatcgtgacc gagccgctgc ggctgacgtc gaccgaggag acctacgacg
     2701 tcgaggtgac catggacggg ggcggcgtga gcgggcaggc gggcgccctg cgccacggga
     2761 tcgcccgggc cctggtcgag ctcgaccccg agctgcgcat ctcgctcaag cgggccgggt
     2821 tcctgacgag ggacgaccgc aagaaggaga gcaagaagta cggcctgaag aaggcccgca
     2881 aggccccgca gtactccaag cggtagcggg ctggttgcgc ccgtcgttcg gcaccgacgg
     2941 cgtccgcggc cgggccaatg ccgagctgac gcccgagctc gtcctggccc tcggccgggc
     3001 cgcggcccgc cggctcggcc ccggcccgat gctcgtgggc cgcgacaccc gccgttccgg
     3061 gcccatgctg caggcggccc tgacggccgg gctggcgagc gagggcatgg acgtggtcga
     3121 cctcggcgtg ctgccgacgc ccggcgtggc cttcctgtcg cagcggaggg accagccggc
     3181 ggcgatgatc tcggcgtcgc acaacccctt cgcggacaac ggcgtcaagt tcttcctggc
     3241 cgggggccgg aagctgccgg tccaggtcga ggcggggctg gaggcggagc tggccggcat
     3301 cctcgaggac cgcgaccgcg accatgaccg cgaccgcggc cacccgggcg ccgcccgccc
     3361 cgagggacgg ggtgtgggtg gcgccggccc cgagccaggc gcggcggagg agtacacccg
     3421 ccacctggtc tcgacggtgc cggccggtag cggcctgggc ggtctcagcg tggtgatcga
     3481 ctgcgcccac ggggccgcca gcgccctcgc cgccggcgtc ttccgccagg ccggcgccga
     3541 ggtgctcgtg ctgtcggcgc agcccgacgg ctgcaacatc aacgaccgct gcgggtccac
     3601 cgacccgggg agcctcgggc gcgcggtaat cgaaggcggc gcggacctcg gcctcgcctt
     3661 cgacggggac gccgaccgcg tcgtcgccgt cgaccatcaa ggcacgctcg tcgacggaga
//
LOCUS       DASWGG010000405        10227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_582182, whole genome shotgun sequence.
ACCESSION   DASWGG010000405 DASWGG010000000
VERSION     DASWGG010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582182"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..608)
                     /locus_tag="VF954_07480"
     CDS             complement(72..608)
                     /locus_tag="VF954_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX9530962.1"
                     /translation="MNRSPLLVNVAPLRRRVGSRRREQRTARLAGLAVIGSRVREGEE
                     VAVDVLLESVHHGILASGTVSAGWEAECRRCLGPARGQLTVAVRELYEETPRADAGDD
                     DTYPLTGDQLDLAPLARDAVLLELPLAPLCTEACLGLCPTCGANRNEQRCSCEAPRDE
                     RWAALEVLRGGGPGRPAE"
     gene            complement(605..1252)
                     /locus_tag="VF954_07485"
     CDS             complement(605..1252)
                     /locus_tag="VF954_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530963.1"
                     /translation="MSFSESNHDGSQPDTETLLRRVVEIIGNAKSMPLSNSVIISKDE
                     VLELLEDGIERLPPELQQARWMLKEREEYLARVKSDGDEILDAARVRAERMVQRTEIV
                     RQAQHTAERTSQRANDEASRLRLEAEDYCDQKLAAFEIVLERTMKQVQGGRQRLRVTP
                     LPPAEAGDLVGELDGSFTGAFGDPHGDPRGDMHGATRAVGAGGVEDEGFFDQDRV"
     gene            complement(1249..1734)
                     /gene="coaD"
                     /locus_tag="VF954_07490"
     CDS             complement(1249..1734)
                     /gene="coaD"
                     /locus_tag="VF954_07490"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX9530964.1"
                     /translation="MKTALFPGSFDPFHNGHLEIVERASKLFDKIVVAAMRNPQKGEA
                     LFDLAERKQMIEDSVAGAGVRNVEVVSLARLVVDVAAEVRASAIIKGLRAVVDFENEM
                     QMAQMNHRLSGIDTLLMPTGPTTSFIAAKLIREVARYGGDVSELVPPPVARRLKEKFA
                     P"
     gene            complement(1731..2303)
                     /gene="rsmD"
                     /locus_tag="VF954_07495"
     CDS             complement(1731..2303)
                     /gene="rsmD"
                     /locus_tag="VF954_07495"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX9530965.1"
                     /translation="MRVVGGDAGGRRLHAPAGRRTRPTSDQVREAVFNMLANLGGVEG
                     KVVIDLFAGTGALGIEALSRGARSATFVEQDPRTVAVLRRNLEVIADRRATVEVATAD
                     VLGWLGRQAGHPSRTSYDVAFADPPYRFDLWNRLMAALMDARVLLAVLESEVPVAVPD
                     AWEIVREKRYGGTVVTLIQPLPAVTSKGPM"
     gene            2350..2778
                     /locus_tag="VF954_07500"
     CDS             2350..2778
                     /locus_tag="VF954_07500"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9530966.1"
                     /translation="MEGDCFGCGDDNPVGLGLQFSVAPPGLVASFLPRPEHRGGPGRL
                     HGGLAATCLDETMGALGFALDGTHCVTATLDLRYRRPVPIDGTAVRVEAWRERPEARR
                     RQKVHGRLLLADGRVAVEATGIFVQVGAGEGGGPWEGVVR"
     gene            2775..2954
                     /locus_tag="VF954_07505"
     CDS             2775..2954
                     /locus_tag="VF954_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530967.1"
                     /translation="MSSSPLVDPVITCVDCGGRAHLLTTWPEDDPPIPGDVVSYRCSD
                     CWDRWDLVVADDGSD"
     gene            complement(3004..3234)
                     /locus_tag="VF954_07510"
     CDS             complement(3004..3234)
                     /locus_tag="VF954_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530968.1"
                     /translation="MPRRDDSPALASNWVVVLLADGLVGVGVAAVGGWTMARGSLPIG
                     AALVAAAVLYLGLIVGRGMRWRRLRREAGPGS"
     gene            3317..4240
                     /locus_tag="VF954_07515"
     CDS             3317..4240
                     /locus_tag="VF954_07515"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX9530969.1"
                     /translation="MRRSETTKSPQLRLVRIAAGQWGVATLAQAVGCGVHHDTMQSWA
                     RQGKIERLRSGIYALPGFPGCFEQRLLACVLAGGPRTVASHHSAARLYGLDGFDRTSE
                     LHVLTPRGVRVKGARAHQTPDLARHDRQTVSGIPATSPTRTLVDLADVVDRVELEQAL
                     ECALRRHLTTVAFATRRAAALTRRGARGPYLLGLLLARRGDVPATSSMAETVFLQLVR
                     AAGLAEPVRQHPVGRRLLDFAWPPARVAVEIDGGEAHVGRAALDRDLVRQNAIVLEGW
                     RILRYSATRVLDHRLHPQILTELAATLERAA"
     gene            complement(4249..6372)
                     /gene="recG"
                     /locus_tag="VF954_07520"
     CDS             complement(4249..6372)
                     /gene="recG"
                     /locus_tag="VF954_07520"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX9530970.1"
                     /translation="MRRLAQLDALPVTKLDGVGPKKAAALEELGITSVLALVTHYPRR
                     YIDRTQQAEIRDLEVGEEAMVLARVKRSSSRRTRQGRALVEVDVFDGSGYLRCVFFNQ
                     GWRARQLAVGTEAVFFGKVETFQGRRRMTSPVVDLIGDRTGRIVPVYPQSEKAGLTTW
                     ELGGWIEEALTRAGELVDPLPRSWQEELGLVSRTWAMHQIHLPETTEAAFAARKRLAF
                     DELLRLQMTLVMRKRAVEREAKGIRHQASGRLVRIFHESLPFRLTGAQQRAIADIGAD
                     LAGPHPMHRLLQGDVGAGKTVVAVSALLVAVDGGYQGALMAPTEVLAEQHLLGVRALL
                     SGLVLPEAGSLLGERPVRVDILTNRTTASERARLHAGLRSGEVDILIGTHALLTEQVE
                     FHALGVVVIDEQHRFGVEQRSALRGKGSDPDVLVMTATPIPRTAAMTVYGDLDVTVLD
                     ELPPGRAPVRTTWARGPLEEEAAWARVRTEVAAGRQAYVVCPLVEESERVQARSATEE
                     LERLRADVLPELRLGLLHGQMPSRDKESVMAAFRRGEIEVLVATTVIEVGIDVPSATV
                     MVIEDADRFGIAQLHQLRGRVGRAADQSWCFLLGGGSGLGGGSSSDGEVRLRAVEATT
                     DGFELAEVDLEVRGEGTILGTRQKGRTDLKLASLRRDKDLVRQAREVAFSIVDGDPML
                     AAHPDLAQEVRYLVDDEEAEFLFKS"
     gene            complement(6389..8029)
                     /locus_tag="VF954_07525"
     CDS             complement(6389..8029)
                     /locus_tag="VF954_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX9530971.1"
                     /translation="METLERLTAGDLRATVVGYRDALRAHQDVIDRLNVYPVPDGDTG
                     SNMALTVESVLTELKAAGDDMGATCQAISHGSLMGARGNSGVILSQILRGLAEAFRDH
                     DAGDAGLMAAALRDASGRAYAAVMRPVEGTILTVVRAASDASQEAASAGKGLVEVLDA
                     AKRAGAEALEATPELLPALKQAGVVDAGGAGFLLLLDAFLYVTAGRPLPEPRTAAGPG
                     VVGRAAGPHASDADGLRYEVMYFLEAPDDAVPAFREVWAGIGDSIVVVGGDGIWNCHI
                     HTDDIGAAVEAALDAGRPRDIRVTDLLEQVEEERWVREARPASTDDEGPYEPVACAVV
                     AIGTGDGVRRIFRSLGVHHIVAGGQSMNPSTAQILEAVEAVPADEVVVLPNNSNIVPV
                     ARQVAALTGKSVRVVPTRGIAEGFAALLEYDPEASAEDNARSMGDSALRIVAGEVTRA
                     VRDSDSAAGAIRAGDWLGLAREGIVVVRPTLEEAATCLLDQLVGEGHEIVTLIEGEGA
                     TPAVTRRLTEWLADRRPGVTAEVHHGGQPLYPYVFSVE"
     gene            8086..8280
                     /gene="rpmB"
                     /locus_tag="VF954_07530"
     CDS             8086..8280
                     /gene="rpmB"
                     /locus_tag="VF954_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX9530972.1"
                     /translation="MAAVCEVCGKHPSFGMNVSHSQRHTKRRWSPNIQRVRALVNGAP
                     KRMHVCTSCIRAGKIDKAVH"
     gene            complement(8309..9112)
                     /gene="thiD"
                     /locus_tag="VF954_07535"
     CDS             complement(8309..9112)
                     /gene="thiD"
                     /locus_tag="VF954_07535"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX9530973.1"
                     /translation="MGDRRPPVALTVAGTDSGGGAGVAADLRTFCAHRVWGAVAVTAV
                     TAQDTLGVQAVEPVAPAVIRAQIDSVLGDLGADAVKTGMLGSEAAVRAVAAGLRSRGA
                     GPLVVDPVLRATTGRALLADEALACLTEVLLPLAALVTPNLAEAAALAGVPAVVDRDG
                     MVGAAERILRTGAGAVLVTGGHLVGERAADCLLTPGAAPRWLVSDRIDDAGTHGSGCV
                     LSAAVTARLALGDELGEACAAAKRYVTAAIAASVRVGRGEAAVSPPACC"
     gene            complement(9105..10037)
                     /gene="thiL"
                     /locus_tag="VF954_07540"
     CDS             complement(9105..10037)
                     /gene="thiL"
                     /locus_tag="VF954_07540"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX9530974.1"
                     /translation="MDSTGGGEWAAIGRIRSRFPAPPPGETWIGDDAAVVEVAGHALL
                     LAADVVVAGTHVDLALTSIADMGWKAVAVNVSDIAAMGGRPLHLTVTVVGPPEIDLDG
                     LYDGIAEASRHYGCPVVGGDLAIGPGPSVSVAITGWSPERRPVLRAGARPGDGLWCTG
                     PLGRASAGLSDLRAGRASSANAAAHRRPRARVEEGTAAARAGATAMTDVSDGLASDLH
                     HLVQASATGAALDRVPVAEGCTTEQALGGGEDYELVFAAPDPDRVRAEFAAAGLATPL
                     LIGRCVPAADGLTLAGQPLAPLGWEHRWRGASRG"
BASE COUNT         1372 a         3850 c         3658 g         1347 t
ORIGIN      
        1 ggccatggtg cgctctcgct ctcgtcgtgg ctgctgggct gcggctgttg ctcgggctgt
       61 tgctcgggcg gttactcggc gggccggccg gggccgccgc cgcgcaggac ctcgagcgcc
      121 gcccaccgct cgtcccgggg agcctcgcag gagcagcgct gctcgttgcg gttggccccg
      181 caggtcgggc acagccccag gcacgcctcg gtgcagaggg gggccagggg aagttccagc
      241 aggacagcgt cgcgcgctag cggcgccagg tcgagctggt cgccggtcaa aggataggtg
      301 tcgtcgtcgc cggcgtcagc ccgaggggtc tcctcgtaca gctcgcggac cgcgacggtg
      361 agctggccac gggccgggcc gaggcaccgg cggcactccg cctcccaccc ggccgacacc
      421 gtgccgctcg ccagaatgcc gtggtggacg gactcgagca gcacgtccac ggcgacctcc
      481 tcgccctccc ggacccggct gccgatgacg gccaggccgg ccagccgcgc cgttcgctgc
      541 tcgcgccggc gcgaccccac ccggcgccgc agcggggcca cgttgacgag caacggcgac
      601 cggttcatac ccggtcttgg tcgaagaacc cttcgtcctc cacgccgcca gcgcctaccg
      661 ctctcgtggc cccgtgcatg tcgccccgcg gatcgccgtg cggatcgccg aaggcccccg
      721 tgaagctccc gtcgagctca cccaccaggt cacccgcctc cgcgggaggc aggggcgtga
      781 cccgcaaccg ctggcgcccg ccctggacct gcttcatcgt ccgctccagc acgatctcga
      841 aggcggccag cttctggtcg cagtagtcct cggcctcgag ccgcagccgg ctggcctcgt
      901 cgttggcccg ctggctcgtc cgctccgcgg tgtgctgggc ctggcgcacg atctccgttc
      961 gctggaccat gcgctcggcc cggacccggg cggcgtcgag gatctcgtcg ccgtcgctct
     1021 tcacccgggc caggtactcc tcgcgctcct tcagcatcca cctggcctgc tggagctccg
     1081 gcggcaggcg ttcgatgccg tcctcgagca gctcgagcac ctcgtccttg ctgatgatca
     1141 ccgagttgga caggggcatg gacttggcgt tgccgatgat ctcgaccacc cgccgcagca
     1201 gggtctcggt gtcgggctgg gaaccgtcgt ggttggactc ggagaagctc acggggcgaa
     1261 cttctccttc agccgccggg ccacgggcgg cggcaccagc tcgctgacgt cgcccccgta
     1321 ccgggccacc tcccggatga gcttggccgc gatgaacgag gtggtcggcc cggtcggcat
     1381 gagcagggtg tcgatgccgg agaggcgatg gttcatctgg gccatctgca tctcgttctc
     1441 gaagtcgacc acggcccgta gccccttgat gatggcgctg gcccgtacct cagcagccac
     1501 gtcgacgacc aggcgggcca gggagaccac ctcgacgttg cgcaccccgg cgccggcaac
     1561 gctgtcctcg atcatctgct tgcgttcggc caggtcgaag agggcctcgc ccttctgcgg
     1621 gttgcgcatg gcggccacga cgatcttgtc gaagagcttc gaggcacgtt cgacgatctc
     1681 gaggtgcccg ttgtggaagg gatcgaacga gcccgggaag agggctgtct tcacatcgga
     1741 cctttcgacg tgaccgcggg cagtggttgg atcagggtca ccaccgtacc gccgtagcgc
     1801 ttctcacgga caatctccca ggcatccggg acggcgacag ggacctccga ctccagcacc
     1861 gcgagcagca cccgggcgtc catgagggcc gccatcaggc ggttccacag gtcgaaccgg
     1921 tacggtgggt cggcgaaagc cacgtcgtag gatgttcggg atggatggcc ggcttgacgc
     1981 ccgagccacc ccaggacatc ggcggtcgcc acctcgaccg tggcgcgccg gtccgcgatc
     2041 acttcgaggt tccgcctgag cacggcgacc gtgcgagggt cctgctccac gaaggtggcc
     2101 gaccgcgccc cccgcgaaag ggcttcgatc ccgagggcgc cggtgccggc gaacaggtcg
     2161 atcacgacct tgccctcgac gccacccagg ttcgccagca tgttgaagac ggcctcgcgg
     2221 acctgatcgc tcgtcggcct cgtccgacgc cccgccgggg cgtggaggcg ccggcctccg
     2281 gcatctcctc ccaccacgcg cacgggccca gggtaagggg gtgtcggctc ggcgactacc
     2341 gtttggcggg tggaaggcga ctgcttcggc tgcggcgacg acaaccccgt cgggctgggc
     2401 ctgcagttct cggtggcgcc gcccggcctg gtcgcgtcct tcctgccccg gccggagcac
     2461 cggggggggc ccggacggct gcacggcggg ttggccgcca cctgcctgga cgagaccatg
     2521 ggggccctcg gcttcgcgct cgacggcacg cactgcgtca cggccaccct cgaccttcgc
     2581 taccgacggc ccgtaccgat cgacgggacg gccgtgcggg tcgaggcctg gcgggagcgg
     2641 cccgaggcgc gccggcgcca gaaggtgcac ggccggctcc tgctggccga cggccgggtg
     2701 gcggtggagg cgaccggcat cttcgtgcag gtgggggccg gcgagggcgg ggggccgtgg
     2761 gaaggcgtgg tccggtgagc tcgtcgcctc tcgtggaccc tgtcatcacc tgcgtcgact
     2821 gcgggggccg ggcccacctg ctgaccacct ggccggagga cgatccgccc atccccgggg
     2881 acgtggtgag ctaccgctgc tccgactgct gggaccgctg ggacctggtc gtggccgacg
     2941 acggcagcga ttgacccgcc tgctgcgacc gatctccggt cactcgattc tcgagtcact
     3001 cgatcaggag ccgggcccgg cctcccggcg taggcggcgc caccgcatcc cccggccgac
     3061 gatgagcccc aggtagagga cggcggcggc gaccagggcg gccccgatgg gcagcgatcc
     3121 ccgggccatg gtccacccgc ccaccgccgc cacccccacg ccgaccaacc cgtcggcgag
     3181 cagcaccacg acccagttgg aggcgagcgc cggtgagtcg tcccgtcgtg gcatgcccca
     3241 ccttcccacg cccggtccgc cccgcgccga gcccgaccct atggacaatt tcatgtcatt
     3301 tcattttact ggggagatga gacggtctga gaccaccaaa tctccccagc tacggctcgt
     3361 gcgcatcgcc gcggggcagt ggggcgtcgc cacccttgcc caggcggtgg gttgcggagt
     3421 ccaccacgac acgatgcaga gctgggcgcg ccaggggaag atcgagcgcc tccgcagcgg
     3481 gatctacgcg ctgcccggct tcccgggctg cttcgagcag cgcctcctgg cctgcgtgct
     3541 ggcgggaggg ccgcggacag tggccagcca ccacagcgcg gcccggctgt acggcctgga
     3601 cgggttcgac cgcacgagcg agctgcacgt gctcacgccg cggggcgtgc gagtcaaagg
     3661 cgccagggcc caccagacgc cggacctggc ccgccacgac cgccagaccg tgagcggcat
     3721 ccccgcgacc agccccaccc ggaccctggt cgacctggcc gacgtggtgg accgagtcga
     3781 gctcgagcaa gcgctcgagt gcgccctccg caggcacctg accaccgtgg ccttcgccac
     3841 caggcgcgct gcggccctca cccggcgcgg cgcacggggg ccctacctgc tcgggctgct
     3901 gctggcccgg cggggcgacg tcccggccac gagcagcatg gccgagacgg tcttcctgca
     3961 gctcgtccgc gccgccgggc tggccgagcc ggtgcgccag caccccgtcg gccggcggct
     4021 gctcgacttc gcctggccac cggcccgggt cgccgtcgag atcgacggtg gcgaggcgca
     4081 cgtgggccgg gccgccctcg accgcgacct cgtgcgacag aacgccatcg tgctggaggg
     4141 atggcgcatc ctgcgctact cggccacccg ggtgctggac caccgcctcc acccccagat
     4201 cctgaccgag ctggccgcca ccctcgaacg cgccgcgtga cggcggcgtc aggacttgaa
     4261 gaggaactcg gcctcctcgt cgtcgaccag gtaccgcacc tcctgggcca ggtcggggtg
     4321 ggcggcgagc atgggatcgc cgtccacgat cgagaaggcg acctcgcggg cctggcgcac
     4381 gaggtccttg tcccggcgca gcgaagccag cttgaggtcg gtacggccct tctggcgggt
     4441 cccgaggatg gtgccctctc cccgcacctc gagatcgacc tcggccagct cgaaaccgtc
     4501 ggtcgtggcc tcgacggcgc gcaaccggac ctccccgtcg gagctcgacc cgcccccgag
     4561 acccgacccg cccccgagga ggaagcacca ggactggtcc gcggcccgcc ccacccggcc
     4621 gcgcagctgg tggagctggg cgatgccgaa gcggtcggcg tcctcgatga ccatcaccgt
     4681 cgccgacggg acgtcgatgc cgacctctat cacggtggtg gccacgagca cctcgatctc
     4741 gccccgccgg aaggcggcca tgaccgattc cttgtcccgc gagggcatct gaccgtgcag
     4801 cagcccgagg cgcagctcgg gcagcacatc ggcgcgcagc cgctcgagct cctcggtcgc
     4861 cgaccgggcc tggacccgct cggactcctc caccagcggg cacacgacgt aggcctggcg
     4921 gccggcggcc acctcggtcc gcacccgggc ccaggccgcc tcctcctcga gcgggccccg
     4981 cgcccatgtc gtccgtacgg gagcccgccc cggcggcagc tcgtcgagca ccgtgacgtc
     5041 gaggtccccg tacacggtca tggccgccgt gcgggggata ggggtggccg tcatgaccag
     5101 gacgtccggg tcgctcccct tgccgcgcag agccgaccgc tgctcgaccc cgaagcggtg
     5161 ctgctcgtcg atgaccacta cgcccagggc gtggaactcg acctgctcgg tcaacagcgc
     5221 gtgcgtcccg atgaggatgt ccacctcgcc ggaccgcagg ccggcgtgca ggcgggcccg
     5281 ttccgatgcg gtcgtccggt tggtgaggat gtcgacgcgc accgggcgct cgccgagaag
     5341 cgagcccgcc tccggcaaca ccagccccga caggagcgcc ctcacgccga ggaggtgctg
     5401 ctcggccagc acctcggtcg gcgccatgag ggcgccctgg taaccgccgt cgaccgccac
     5461 gagcagggcc gagacggcca cgacggtctt gcccgccccc acgtcgccct gcaggagccg
     5521 gtgcatgggg tggggccccg ccaggtcggc cccgatgtcg gcgatggccc gctgctgggc
     5581 gccggtgaga cgaaagggca gggactcgtg gaagatccgc accagccgcc cggacgcctg
     5641 gtgccggatg cccttggcct cgcgctcgac ggcccgcttg cgcatcacca gggtcatctg
     5701 gagccgcagc agctcgtcga aggccagccg cttgcgggcg gcgaacgcgg cctcggtggt
     5761 ctccggcagg tggatctggt gcatggccca ggtccgggac acgagtccga gctcctcctg
     5821 ccacgaacga ggcaacgggt cgaccagctc accggcccgg gtgagcgcct cctcgatcca
     5881 gccgccgagc tcccaggtcg tgagcccggc cttctccgac tgcggataca cgggcacgat
     5941 gcgcccggtg cggtctccga tgaggtccac gaccgggctg gtcatgcgcc ggcggccctg
     6001 gaaggtctcc accttcccga agaagacggc ctcggtgccc accgccagct gccgagcccg
     6061 ccagccctgg ttgaagaaca cgcaccggag gtagccgctc ccgtcgaaca cgtccacctc
     6121 gaccagcgcc cggccctggc gggtgcggcg cgacgagctg cgcttgaccc gggccaggac
     6181 catggcctcc tcccccacct cgaggtcgcg gatctcggcc tgctgggtcc ggtcgatgta
     6241 gcgcctggga tagtgggtga cgagggcgag gacgctggtg atgccgagct cctcgagggc
     6301 ggcggccttc ttgggcccga ccccgtcgag cttggtcacg ggcagggcgt ccagttgggc
     6361 gaggcgcctc atgcggccat tcgaccagct attcgacgct gaagacgtac gggtaaagcg
     6421 gctggccccc gtgatgaacc tcggcggtca cgcccggccg ccggtcggcc agccactctg
     6481 tcaaccgcct cgtcaccgcc ggcgtggcgc cctcgccctc gatcagggtc acgatctcgt
     6541 gaccctcgcc gaccagctgg tcgagcaggc aggtggcggc ctcctcgagc gtggggcgca
     6601 ccaccacgat cccctcccgc gccagcccga gccagtcgcc cgcccggatg gcgccggcgg
     6661 cggagtcaga gtcgcggacg gcccgggtca cctcgcccgc cacgatccgc agggccgagt
     6721 cgcccatcga ccgggcgttg tcctcggccg aggcctccgg gtcgtactcc aacagggccg
     6781 cgaagccctc ggcgatgccc cgggtgggca cgacccgaac ggacttgccc gtgagcgcgg
     6841 ccacctgccg ggcgaccggg acgatgttgc tgttgttggg gaggacgacg acctcgtcgg
     6901 cgggcacggc ctcgaccgcc tcgaggatct gggcggtcga ggggttcatc gactggccgc
     6961 cggcgacgat gtggtggacg ccgagcgacc ggaagatccg cctgaccccg tcgcccgtgc
     7021 cgatcgcgac cacggcgcag gccaccggct cgtacgggcc ctcgtcgtcg gtcgatgccg
     7081 gccgggcctc gcgcacccac cgctcctcct cgacctgctc cagcaggtcg gtcacccgga
     7141 tgtcgcgcgg ccggcccgcg tcgagggcgg cctcgaccgc ggcgccgatg tcgtcggtgt
     7201 ggatgtggca gttccagatg ccgtcgccgc cgaccaccac gatcgagtcg ccgatgccgg
     7261 cccagacctc ccggaacgcg gggacggcgt cgtcgggcgc ctcgaggaag tacatgacct
     7321 cgtagcgcag gccgtcggcg tcggaggcgt ggggcccagc cgcacgcccc accacacccg
     7381 gcccggccgc cgtgcggggc tcgggcagcg ggcggcccgc ggtcacgtac aggaaggcgt
     7441 cgagcaacag caggaacccg gcgcctcccg cgtccaccac gcccgcctgc ttgagggcgg
     7501 ggagcagctc cggggtcgcc tccagcgcct cggctccggc ccgcttggcg gcgtcgagga
     7561 cctcgacgag gcccttcccg gcgctcgccg cctcctggct ggcgtcggac gcggcccgca
     7621 cgaccgtgag gatcgtgcct tcgaccggtc gcatcacggc cgcgtacgcc cggccgctcg
     7681 cgtccctgag cgcggccgcc atgagcccgg catcgccggc atcatggtcg cggaaggcct
     7741 cggccagccc ccgcagtatc tgcgacagga tcacgcccga gttgccgcgg gcgcccatca
     7801 gggagccgtg gctgatcgcc tggcaggtgg cgcccatgtc gtcgcccgcc gccttcagct
     7861 cggtcagcac ggactccacg gtgagggcca tgttggagcc cgtgtcaccg tcgggcaccg
     7921 ggtagacgtt gagccggtcg atcacgtcct ggtgggcccg gagcgcgtcg cggtagccga
     7981 ccacggtggc ccgaaggtcg ccggccgtca gccgctcgag cgtctccatg ggctgcgatg
     8041 gtaacctcac gccgtcccct ggaatcccgt tggaaggtgc gcgtcatggc agcggtttgc
     8101 gaggtgtgcg gaaagcatcc ctctttcggc atgaacgtca gccactccca acggcacacc
     8161 aagcgacggt ggagccccaa catccagcgg gtgcgcgccc tcgtcaacgg cgcccccaag
     8221 cggatgcacg tctgcacctc ctgcatccgg gccggcaaga tcgacaaagc cgtccattag
     8281 cagggccgtc cattagcagg gccgcccatc agcagcaggc cggggggctg accgcggcct
     8341 cccctcgccc gacgcggacg ctcgcggcga tggccgccgt cacataccgc ttggccgccg
     8401 cgcaggcctc cccaagctcg tcgcccaggg ccaggcgcgc cgtcaccgca gccgacagga
     8461 cgcacccgct cccgtgcgtc ccggcgtcgt cgatccggtc ggacaccagc cacctggggg
     8521 cggcgccggg ggtgaggagg caatcggcag ccctttcccc gaccaggtgc ccgcccgtca
     8581 cgaggacggc gccggcgccg gtgcgcagga tgcgttcggc ggcgccgacc atcccgtcgc
     8641 ggtccaccac cgcgggcacg ccggcgaggg cggcggcctc ggccaggttc ggcgtcacga
     8701 gggcggcgag gggcaggagg acctcggtca ggcaagcgag ggcttcgtcc gccagcagcg
     8761 cccgtccggt cgtggcccgc agcaccgggt cgacgacgag ggggccggcg ccacgcgagc
     8821 gcagcccggc cgcgaccgcc cgcaccgccg cctcgctgcc cagcatcccg gtcttgacgg
     8881 cgtcggcccc gaggtcgccg agcacgctgt cgatctgggc gcggatcacg gccggggcga
     8941 ccggctccac ggcctgaacc ccgagggtgt cctgcgccgt caccgcggtc accgccaccg
     9001 cgccccacac ccggtgggcg cagaaggtgc gcaggtcggc ggccacgccg gcgccgccgc
     9061 ccgagtccgt gccggccacc gtcagggcca ccgggggacg gcggtcaccc acgggaggcg
     9121 cctcgccagc ggtgctccca ccccagtgga gccagcggct ggccggcgag ggtcaacccg
     9181 tcagccgcgg ggacgcagcg gccgatgagc aggggcgtcg ccaggcccgc ggcggcgaac
     9241 tcggcgcgca cccggtccgg gtccggggcg gcgaagacca gctcgtagtc ctcgcccccg
     9301 ccgagcgcct gctcggtcgt gcagccctcg gccaccggca cgcggtcgag cgcggcgccc
     9361 gtcgccgagg cctggaccag gtgatggagg tcggacgcca gcccgtcgga cacgtccgtc
     9421 atggcggtgg cgcccgcccg ggcggccgcc gtcccctcct ccacgcgggc ccggggccgg
     9481 cggtgggcgg cggcgttggc ggaggaggcc cggccggctc ggagatccga gaggcccgcc
     9541 gaggcccgcc ccaacggccc cgtgcaccac agcccgtcgc cgggccgggc gccggcgcgc
     9601 aggaccgggc ggcgctcggg cgaccaaccg gtgatggcta ccgaaacgct cgggcccggg
     9661 ccgatggcca ggtcgccgcc caccacgggg cagccgtagt ggcgggacgc ctcggcgatg
     9721 ccgtcgtaga gcccgtcgag gtcgatctcg ggagggccga cgacggtcac ggtgaggtgg
     9781 aggggccgcc cgcccatggc ggcgatgtcg ctgacgttga cggccacggc cttccagccc
     9841 atgtcggcga tcgatgtgag ggccaggtcg acgtgggtcc cggccacgac cacgtcggcc
     9901 gcgagcagca gggcgtggcc tgcgacctcg accacggccg cgtcgtcgcc gatccacgtc
     9961 tcgcccgggg gcggggcggg gaaacggctg cggatgcgcc cgatggccgc ccactcgccg
    10021 cctccggtac tgtccacggg ccgagcgtag gcccggccca tggcgagagg catggcgaga
    10081 ggcatggcga gaggaggcgc ggtgcaaggc gtggtcaagt cctacgaccc gggcacggga
    10141 gcgggcgtga tcgtgcgcga caccgatcgc caggagctcg acatggccgg gggggcgctc
    10201 gccggctcgg tgttccgcat gctgcgc
//
LOCUS       DASWGG010000406         5420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2188651, whole genome shotgun sequence.
ACCESSION   DASWGG010000406 DASWGG010000000
VERSION     DASWGG010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2188651"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VF954_07545"
     CDS             complement(<1..647)
                     /locus_tag="VF954_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX9530975.1"
                     /translation="MAILGREFIAVPDDRKGQIVFKWHDNNLRRFSRAIVDVDELALF
                     VNQGQVVGTLPPGQHQLDANELPFLGAIIDRVTGGNAYRAELFFVGTREYTSQPFGGR
                     IDDVQDPQTGMIVTLRVFGDYALKVVDPGKLVLNLTGTVDVTDNDAITAWIAEQLLKV
                     MRSGITTQIVRNGWPILGLSAYTADIESSVIASANAQIDTYGLSIVRMGNFDINLS"
     gene            1114..2484
                     /locus_tag="VF954_07550"
     CDS             1114..2484
                     /locus_tag="VF954_07550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9530976.1"
                     /translation="MAALGVRPTAVRAQTATTTTDPSGATGSGGASTGGTTTTTGPPP
                     GAAPGTTTTAPPPAPTTTAAPAPGPAPTTTTPTTTAVANGGDPSRNDPLAGENDPPVA
                     VPTGVVPVPPRAVPRAPTPVDVALAQALAQQAAQAEASANAADVKVAAVEAKVADVQR
                     QVDGLRSQEQDAIWKLAAARTRLVRRAVAAYVGGSIAPLGNLLQVRDINELDRDLQIE
                     NEASRAELASVRDYEAARKDAGSAAGTLTASMDNLSVDLGTAQTEQAASHQVLAATSG
                     QVAAANVDPSLVLDGFVFPVLGPHKLDRNFGEPRNLGTPYFHLHQGDDVEAPQNAPLV
                     AVVRGAIVNISSSALGGNGLWIQGSDGTKYYYAHLAGYAPDMTVGQIVEAGQLVGFVG
                     STGDATGPHLHFEVHPNGGPAIDPWSLLDAIDVIQAETQTSDAPGGPAASPSGGGGPA
                     TTTPAG"
     gene            2562..3392
                     /locus_tag="VF954_07555"
     CDS             2562..3392
                     /locus_tag="VF954_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX9530977.1"
                     /translation="MSYSAEISRANPTCFVILVDRSLSMADPIGGGSRPMRKADAVVQ
                     AINGLLSELAVKCAKDDGVRDYFHVAVIGYGAEITSGFHGPLSGRWLVPLSAVAAMPL
                     RLDERVPAGAGAAGRPVRIPVWVEPVAFGDTPMQAALAQAYAVVREWLAVHPGCFPPI
                     VLNITDGESTDGDPEPVANALRELASADGPVLLFNLHLSTDASDPLVFPADGGRLPDA
                     WARKLCSMSSYLPAHMVEMARRHGLDVSQEGAKGFVFNADVSSVVQFLDIGTQVLNLR
                     "
     gene            3397..4185
                     /locus_tag="VF954_07560"
     CDS             3397..4185
                     /locus_tag="VF954_07560"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX9530978.1"
                     /translation="MEAVFTTQWMPRDGSQMGDYEDAFAPATAGKMAGRRLRFAVADG
                     ATESLLSGPWARLLVDTWVSAPRRSAGQLVGAARLEWDRQLAAYLGARASSDRPVQWY
                     EEPGLARGAHATFVGFQLTSRRVVGASGKWSAVAAGDSCMFHLRGDGVIGAFPIEEAS
                     AFDRTPDLLASREPGPEPRSGPRRKVRLEARFGEWRSGDVFLLATDAFARWFLSCLHD
                     GVPACELTRVTAYDRDEFARWVAARRSDRSLANDDVTVVSVECL"
     gene            4172..>5420
                     /locus_tag="VF954_07565"
     CDS             4172..>5420
                     /locus_tag="VF954_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530979.1"
                     /translation="MSASEGARTPAGRRFPGAAAYREALQHPDLCLSDPVLASSTVEL
                     TDLGLPRAVSGNFATVFQVTDASGRRHALRCFVRCPDDIGERYEALARHLRGIPDEWN
                     VGFDYQERGIRVGREWYPIVRSRWLQGQALSTWISDHLWDADAIAGLAVRFAGLVDRL
                     RHHGVAHGDLQHGNILIDAQGELRLVDYDGMYVPGLAGSPSNELGHRNYQHPRRTAQD
                     WGPHLDHFATWVIYTSLLSLSVDPLLWGRLDAGDEGLLFRWHDFADPEHSAAFACLES
                     RPDSTLAELASTLRDFVSRSVDQLPALPDLSELLPDPLGRPVATGETLAGPGSVATLA
                     GVGLADANCAGLAPGPAREKVPVGQGRPSPGRHESADTSLAAARARVAELTGWRSRAK
                     VDEGDVPALRAEQRQVMDLEASEV"
BASE COUNT          769 a         2004 c         1891 g          756 t
ORIGIN      
        1 gacaggttga tgtcgaagtt gcccatgcgg acgatcgaca ggccgtaggt gtcgatctgg
       61 gcgttggcgg aggcgatcac cgagctctcg atgtcggcgg tgtacgccga cagcccgagg
      121 atgggccagc cgttgcgcac gatctgagtg gtgatgccgc tgcgcatcac cttcaggagc
      181 tgctcggcga tccaggccgt gatggcgtcg ttgtcggtga cgtcgaccgt gcccgtgagg
      241 ttgagcacca gcttgccggg gtcgacgacc ttcagcgcgt agtcgccgaa gacccgcagg
      301 gtcacgatca tgccggtctg ggggtcctgg acgtcgtcga tgcggccccc gaacggctgg
      361 ctcgtgtact cgcgcgtgcc gacgaagaag agctccgccc tataggcgtt gccgcccgtg
      421 acgcgatcga tgatggcgcc caggaacggc agctcgttgg cgtcgagctg atgctggccg
      481 gggggcaggg tgccgaccac ctggccctgg ttgacgaaca gggcgagctc gtcgacgtcg
      541 acgatcgccc ggctgaagcg gcgcaggttg ttgtcgtgcc acttgaagac gatctggccc
      601 ttgcggtcat cgggaactgc gatgaactcc cgtcccaaga tcgccataaa ccctccatac
      661 cctccgagga gaagcggtgc cgcacggcgt gggtgccggc tgcgcccgct ccccagggta
      721 cggcccgcag gacgctgccg cgccatcagg gcgaccaggg ctggcccggc ccgggccgga
      781 cccccgccga tgattcttca atgcttttgt gtcgccccag tggttttttg gctttaagaa
      841 tgatctccgg ccccggcccg acgccaggac cgccccctgg tcccgtactc tttgtcggct
      901 acgcccattt ccatgccggc cgaccggcct gggcctgctc ccgaagggga cgccggcggg
      961 cgcgcgtaat caccgagcac agatcacgga gcacggagca cggagggtgg tgacggcgga
     1021 gcggtgcgaa ccccggggga ccccaggaat ctaggcggcg cggctcccgc ccggaagcgg
     1081 gccgcggccg ttgcggtggc tgccgcggcg tgtatggccg cgctcggcgt gcgccccacc
     1141 gcggtccggg cccagaccgc cacgaccacc accgacccgt ccggagcgac cggctccggc
     1201 ggggcgtcga cgggtggcac gaccacgacc acgggcccgc ctccgggggc ggcgcccggc
     1261 acgaccacga ccgcgccccc accggcgccg acgacgaccg cggcaccggc gcccggcccg
     1321 gcgccgacca ccaccacgcc gacgacgacc gccgtggcca acggcggcga cccctcgcgg
     1381 aacgacccgc tcgccggcga gaacgacccg ccggtcgcgg tgcccacggg cgtcgtaccc
     1441 gtgccgccac gggccgttcc ccgcgcgccg acgccggtcg acgtggcgct ggcccaggcc
     1501 ctcgcccagc aggccgccca ggccgaggcc agcgccaacg cggccgacgt caaggtcgcc
     1561 gccgtcgagg ccaaggtggc cgacgtccag cgccaggtcg acgggctccg cagccaggag
     1621 caggacgcca tctggaagct ggccgcggcc cgcacccggc tcgtgcgccg cgccgtggcc
     1681 gcctatgtgg gtggctcgat cgccccgttg ggcaacctgc tccaggtcag ggacatcaac
     1741 gagctggacc gtgacctcca gatcgagaac gaggccagcc gcgccgagct ggcatcggtc
     1801 cgcgactacg aggccgcccg caaggacgcg ggatcggccg cgggcacgtt gaccgcgagc
     1861 atggacaacc tcagcgtgga cctcggcacg gcgcagaccg agcaggccgc ctcccaccag
     1921 gtcctggcag ccacgtccgg ccaggtcgcg gcggccaacg tcgatcccag cctggtcctc
     1981 gacggcttcg tgttccccgt cctgggcccg cacaagctcg accgcaactt cggggagccc
     2041 cgcaacctcg gcacgcccta tttccacctc caccagggcg acgacgtcga agccccgcag
     2101 aatgcgcccc tggtggcggt ggtccggggg gcgatcgtca acatctcgag cagcgcccta
     2161 gggggcaacg gcctgtggat ccagggctcc gacgggacca agtactacta cgcccacctc
     2221 gccggctacg cacccgatat gaccgtcggc cagatcgtcg aggcgggcca gctggtcggc
     2281 ttcgtcggct cgaccggaga cgccaccggc ccgcacctcc atttcgaggt gcaccccaac
     2341 gggggcccgg ccatagaccc gtggtcgctg ctcgacgcca tcgacgtcat ccaggccgag
     2401 acccagacgt cggacgcgcc cggtggcccg gcggcgtccc cctccggtgg cggcggcccg
     2461 gccacgacga cgcccgcggg ttgacggcct gagcggctca gccgacggcc gcaagccgcc
     2521 gatacaacca gctgagcccc acccgaaagg ttgtcttcgt catgtcgtac tccgccgaga
     2581 tcagccgggc gaaccccacc tgcttcgtca tcctcgtcga ccgctcgttg tccatggccg
     2641 atcccatcgg cggcgggtcc cgccccatgc gcaaggccga cgcagtcgtg caggccatca
     2701 atgggctgct ctccgagctg gcggtgaagt gcgccaagga cgacggagtc cgcgactact
     2761 tccacgtggc ggtgatcggc tacggggccg agatcacatc cgggttccac ggcccgctgt
     2821 cggggcggtg gctggtgccg ctctcggccg tggccgccat gccgctgcgg ctggacgagc
     2881 gggtgccggc cggtgccggc gcagcaggcc gaccggtgcg gatccccgta tgggtcgagc
     2941 cggtcgcctt cggagacaca ccgatgcagg ccgccctggc ccaggcctac gccgtggtgc
     3001 gcgaatggct agccgtccac cccggctgct tcccgcccat cgtcttgaac atcaccgacg
     3061 gcgaatccac cgacggcgac cccgagccgg tagccaacgc cctgcgcgag ctggccagcg
     3121 ccgacgggcc ggtgctgctg ttcaacctgc atctctccac cgatgcgagc gacccgctcg
     3181 tcttcccggc cgacggcggg cggctccccg acgcctgggc ccgcaagctg tgctcgatgt
     3241 cgagctacct accggcccac atggtcgaga tggcgaggcg ccacggtctc gacgtgtcgc
     3301 aagaaggagc gaagggcttc gtcttcaacg ccgacgtgag ctccgtcgtt cagttcctgg
     3361 acatcggcac ccaagtcctg aacctgcgct gaggccatgg aggccgtctt caccacgcag
     3421 tggatgccgc gggacggcag ccagatgggc gactacgagg acgcgttcgc ccccgcgacc
     3481 gcgggcaaga tggcgggcag gcgcctgagg tttgccgtgg ccgacggggc cacggagagc
     3541 ttgctgtcgg ggccctgggc ccggctcctc gtcgacacgt gggtcagcgc tcctcgccgt
     3601 tccgcgggcc agctcgtagg ggccgcccgg ctggaatggg accgccagct cgccgcgtac
     3661 ctcggcgccc gggcgagctc ggaccgcccg gtgcaatggt acgaggagcc cggcctggcg
     3721 cgcggcgctc acgccacctt cgtcgggttc cagctcacgt cgcgccgggt cgtcggtgca
     3781 tcggggaagt ggtcggccgt ggcggccggc gacagctgca tgttccatct acggggcgac
     3841 ggtgtgatcg gggccttccc gatcgaggag gcgtcagcgt tcgaccggac ccctgacctg
     3901 ctcgccagcc gggagccggg ccccgagccc aggagcggcc cacggcggaa ggtgcgactg
     3961 gaggcacgct tcggcgagtg gcgatcaggc gacgtcttcc tgctggcgac ggacgccttc
     4021 gcccgctggt tcctctcctg cctgcacgac ggcgtgcccg cctgcgagct gacccgcgtg
     4081 accgcctacg accgggacga gttcgcccga tgggtggccg cccggcggtc cgatcgaagc
     4141 ctcgccaacg acgacgtgac ggtggtgtcc gttgagtgcc tctgagggcg cgcgaacgcc
     4201 cgcgggccgg cgtttcccgg gcgcggccgc ctaccgcgag gcccttcagc accccgacct
     4261 gtgcctgtcg gacccggtgc tggcgtcgtc cacggtcgag ctgaccgacc tgggcctgcc
     4321 ccgggccgtg agcggaaact tcgccaccgt gttccaggtg acggatgcgt ccgggcgccg
     4381 ccacgccctg cggtgcttcg tacgctgccc ggacgacatc ggagaacggt acgaggcgct
     4441 cgctcgccat ctccggggca tcccggacga atggaacgtc ggtttcgact accaggagcg
     4501 cggcatccgg gtcggccgcg agtggtaccc gatcgtgcgg agccggtggc tacaagggca
     4561 ggcgctgtcg acctggatca gtgatcacct gtgggatgcc gacgccatcg ccggcctggc
     4621 ggtgcgcttc gccggcctgg tcgaccggct tcgccaccac ggcgtggccc atggcgacct
     4681 ccagcacggc aacatcctga tcgacgcgca gggcgagctg cggctcgtgg actacgacgg
     4741 catgtacgtt ccaggcctgg ccgggtcacc gagcaacgag ctcgggcacc ggaactatca
     4801 gcacccccgg cgcacagccc aggactgggg gccgcacctc gaccacttcg ccacctgggt
     4861 catctacacg tcgctcctgt cgctcagcgt cgacccgctg ctctggggcc ggctcgacgc
     4921 aggcgacgag ggcctcctgt tccggtggca cgacttcgcc gacccagagc actcggccgc
     4981 cttcgcctgc ctcgagtccc ggcccgacag caccctcgcc gagctggcgt cgaccctgcg
     5041 cgacttcgtc tcccgctccg tcgaccagct ccctgccctg cccgacctca gcgagctgct
     5101 ccccgacccg ctcggccgcc ccgtcgcgac cggcgagacc ctggcgggcc ccggatcggt
     5161 cgccactctg gcaggggtgg ggttggctga cgccaactgc gccgggctgg cgcccgggcc
     5221 ggcccgggag aaggtgccgg tcggccaggg ccgtccctcc cccggtcggc acgaaagcgc
     5281 cgataccagc ctggccgcgg cgcgcgcccg tgtcgccgag ctcacggggt ggcggtcgag
     5341 agcgaaggtc gacgaggggg acgtgcccgc gctgcgggcc gagcaacgcc aggtcatgga
     5401 cctcgaggca tccgaggtgt
//
LOCUS       DASWGG010000407         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_586483, whole genome shotgun sequence.
ACCESSION   DASWGG010000407 DASWGG010000000
VERSION     DASWGG010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586483"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /locus_tag="VF954_07570"
     CDS             complement(<1..1504)
                     /locus_tag="VF954_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9530980.1"
                     /translation="MKELVYHRYLLPAVERSATQVGFRDGNFEATYELHLDRVLRLAS
                     GMGKVLGLARGDRFAVLALNGHRFEELYHAGLLGAGVVNPLNLRLAPSELELILRDSG
                     TKVCFTDAAFAPLVDRVRSGAGLERVVLIGPGDVPHDVAYEDVIDAGDPVVPDEPEED
                     DPAVLMYTGGTTGLAKGVLIDNRAMMLDLYKVATRWGMHEGFVYLHQTPMFHAASLGA
                     VLAVPAVGATTAFVPMFEPRAVLDAMERFGVTMTVMVPTMIAMLLAHPDFAPESLARL
                     EVLTYGASPMPEELLARLLALFPDTDLYQGYGMTENCGLLTALGPEEHRRGGPILRSA
                     GRPLPGSVVTVQDDHGHLLPPGQTGEVCARAGNYMRGYWNRPEETEAAFRGGWYHTGD
                     AGYLDEQGYVYLVDRVKDMIVTGGENVYSAEVENAIASHPAVAQVAVIGIPSEQWGEA
                     VHAVVVLKAGEKATEEDLTSWSRERIAGFKVPKSVELRTEPLPLSGALKVL"
     gene            complement(1551..>2050)
                     /locus_tag="VF954_07575"
     CDS             complement(1551..>2050)
                     /locus_tag="VF954_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9530981.1"
                     /translation="ELLAVTRALDAAPRPEYLAINVGPATLVDDRFMALMRRRRQGPL
                     VVELTEHAVIDDYAVVIDALDRLRALGIRVAVDDAGSGFSSLRHVISLAPDIIKLDQA
                     LIASIDRDPRRRMLAVSLASFAAEIGSELVAEGIEREEELRVCEEIGIRFGQGFLLGR
                     PAARS"
BASE COUNT          303 a          716 c          742 g          289 t
ORIGIN      
        1 tgagcacctt gagcgccccc gaaagcggca gcggctcggt ccgcagctcc accgacttgg
       61 gcaccttgaa gcccgcgatg cgctcccggc tccaggacgt gaggtcctcc tcggtcgcct
      121 tttctcccgc cttgaggacc accacggcgt gcacggcctc gccccactgt tcgctgggga
      181 tgccgatcac cgccacctgg gccacggccg ggtggctggc gatggcattc tcgacctccg
      241 cggagtagac gttctcgccg cccgtgacga tcatgtcctt gacccggtcg accaggtaga
      301 catagccctg ctcgtcgagg tagccggcgt cgcccgtgtg gtaccagccc ccccggaagg
      361 ccgcctcggt ctcctccggc cggttccagt acccccgcat gtagttgccg gcccgggcgc
      421 agacctcgcc ggtctggccg ggcgggagga ggtggccgtg gtcgtcctgg acggtcacga
      481 ccgagcccgg aagcggtcgg ccggccgagc gcaggatggg gccgccccgc cggtgctcct
      541 cgggtcccag ggcggtgagc agcccgcagt tctcggtcat cccgtacccc tggtagaggt
      601 ccgtgtccgg gaacagcgcc aggagccggg cgagcagctc ctcgggcatc ggggaggcgc
      661 cgtaggtgag cacctcgagc cgggccaggc tctcgggcgc gaagtccggg tgggcgagca
      721 gcatggcgat catggtgggg accatcacgg tcatggtgac cccgaagcgc tccatggcgt
      781 cgagaaccgc cctgggctcg aacatcggca cgaaggccgt ggtggcgccg accgccggca
      841 cggcgagcac cgcgccgagg gacgcggcgt ggaacatcgg cgtctggtgg aggtacacga
      901 agccctcgtg catcccccag cgagtggcca ccttgtacag gtcgagcatc atggcccggt
      961 tgtcgatcag gacccccttg gccaggcccg tggtcccgcc ggtgtacatc agcacggcgg
     1021 ggtcgtcctc ctcgggctcg tcgggcacga ccggatcacc ggcgtctatg acgtcctcgt
     1081 aggccacgtc gtggggcacg tcgccgggcc cgatcagcac aacgcgctcc aggcccgccc
     1141 cggaccggac ccggtcgacg aggggggcga acgcggcatc ggtgaagcac accttcgtgc
     1201 ccgagtcgcg caggatgagc tcgagctctg aaggggccag ccgaaggttg agcgggttga
     1261 cgacgccggc gcccagcagg ccggcgtggt acagctcctc gaaccggtgg ccgttgaggg
     1321 ccagcaccgc gaagcggtcc ccgcgggcca ggcccagcac cttgcccatc cccgacgcca
     1381 gtcgcagcac acggtcgagg tggagctcat aggtggcctc gaagttgccg tcgcgaaagc
     1441 cgacctgggt ggcggaccgc tccacggccg gaaggaggta gcggtggtag acgagctctt
     1501 tcatcgcccc agtcttgccg accgcagccc gggccagtcc gggcctgggc tcaggacctg
     1561 gcggccggcc ggccgagcag gaacccctgg ccgaagcgga tgccgatctc ctcgcagacc
     1621 cgaagctcct cctcccgctc gattccctcg gccacgagct cggagccgat ctccgcagcg
     1681 aagctggcca ggctgacggc gagcatccgc cggcgcgggt cccggtcgat cgatgcgatc
     1741 agggcttggt cgagcttgat gatgtcgggt gcgagggaga tgacgtggcg cagtgacgag
     1801 aagccgctgc cggcgtcgtc gacggccacc cggatgccca gcgcgcggag ccggtcgagg
     1861 gcgtcgatga ccacggcgta gtcgtcgatg acggcgtgct cggtgagctc gacgacgagc
     1921 gggccctgcc ggcgccggcg catgagcgcc atgaaacggt cgtcgaccag ggtggccgga
     1981 cccacgttga tggccaggta ctcgggccgg ggggcggcgt cgagggcccg ggtcaccgcc
     2041 agcagctcca
//
LOCUS       DASWGG010000408         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2191263, whole genome shotgun sequence.
ACCESSION   DASWGG010000408 DASWGG010000000
VERSION     DASWGG010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2191263"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VF954_07580"
     CDS             <1..520
                     /locus_tag="VF954_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9530982.1"
                     /translation="NLFAIAAAAGVGERLLPCLSKVVVAGVGPVTAMTLDRLGVHGYV
                     APPRGRLGLMARQLAHAAAGRGHHLLAGGGEVVVRGSVVRGQGARVDVPERERAVLDV
                     LLRRPGAVVPRAALLKELWGKGSDPKSVDSAIARLRRRLAPTGLTIQTLPRRGYRLDA
                     TVLETTASQGGL"
     gene            complement(501..1388)
                     /locus_tag="VF954_07585"
     CDS             complement(501..1388)
                     /locus_tag="VF954_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530983.1"
                     /translation="MSPYGPLRRRLAVACGLLASASFGTLVGASRSTTAPLALSPVVA
                     LPVERLLPRTTTTAAAPRQHAARPQARPMGSGGGVVAEPPPASTSFGATGASVIVTTT
                     TSPPSPSLRPPVPGRYTYQERVDGQEGQSVLVVDPDGSQTEIRGGRSRLDRVSWTATA
                     RRVTASDWGDGTACTWSPPLLTLALPLTAGQAWDANSTCTASGRAEQRETADVKGRTT
                     VQLDGASMEAWLIERRTTVTETDADGSVATVEQQSSELFAPTIGLVVYRTAKTAVPRP
                     DGTVEESDVVSQLVTGHPG"
     gene            complement(1402..>1969)
                     /locus_tag="VF954_07590"
     CDS             complement(1402..>1969)
                     /locus_tag="VF954_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9530984.1"
                     /translation="AAVTVAAGDTITWTNQDDAPHTVTSSGGPATLDSPQLDKGQSWS
                     YTFTRTGTYNYYCAVHPDMKASVTVAPSPPATTAPVAAGAVAPATAGPPALPLSHPVS
                     PPVARPAGRLSATSPPPGLPASTPTASTPTTAAVVRDDPVPIQAVTTTTGPTINPLLI
                     LAATATAVAVFGVVMLGRPARHPEEPSP"
BASE COUNT          239 a          674 c          784 g          272 t
ORIGIN      
        1 gaacctgttc gccatcgcgg ccgccgcagg cgtaggcgag cgcctgctcc cctgtctgtc
       61 gaaggtcgtc gtcgccgggg tcggaccggt gacagccatg acgctggacc gcctcggcgt
      121 ccacgggtac gtggcgccgc cgaggggccg cctcgggctc atggcgcggc aactggcgca
      181 cgccgcggcc gggcgcggac atcacctcct ggctggcggt ggggaggtgg tcgtccgggg
      241 ctcggtcgtc cgggggcagg gggcgcgcgt cgacgtgccc gagcgcgaac gagccgtgct
      301 cgacgtcctg cttcggcgac ctggggcggt ggtgccgcga gcggctctgc ttaaggagct
      361 ctggggaaag ggcagcgacc cgaagtcggt cgattccgcg atcgcccggc tccgccggcg
      421 gctggccccg accgggctca ccatccagac cttgccccgg cggggctacc ggctggacgc
      481 gaccgtgctc gaaaccacgg ctagccaggg tggcctgtga ccagctggct gacgacgtcg
      541 ctctcctcga ccgtcccgtc cgggcgcggc acggcggtct tcgccgtccg gtagacgacc
      601 agcccgatgg tgggggcgaa cagctcgctg ctctgctgct ccaccgtggc cacgctgccg
      661 tccgcgtcgg tctcggtcac cgtggtgcgg cgctcgatca gccacgcctc catcgacgct
      721 ccgtcgagct gaacagtggt gcgccccttg acgtcggccg tctcccgctg ctcggcccgg
      781 ccgctagcgg tacacgtgct gttcgcatcc caggcctggc cggcggtgag cggcaatgcc
      841 aacgtcagca atggtggcga ccaggtgcag gctgtgccat cgccccagtc ggacgctgtg
      901 acccggcggg cggtcgcagt ccaggagaca cggtcgagcc tggagcgccc accccggatc
      961 tccgtctggc tgccgtcagg atcgaccacc aggacggact ggccctcttg gccgtcgacg
     1021 cgctcctgat aggtgtagcg gcccgggacg ggcgggcgga gcgacggcga tggcgggctc
     1081 gtggtggtgg tgacgatgac ggaggcgccg gtcgcgccaa aagacgtgct cgccgggggc
     1141 ggctcggcca cgacaccacc accactgccc atcgggcgcg cttgcgggcg cgccgcgtgc
     1201 tggcgggggg cggccgctgt ggtggtcgtg cggggtagga ggcgttcgac ggggagggcg
     1261 accaccgggc tgagggccag cggggcggtc gtggatcgcg aggcgcccac cagggtgccg
     1321 aacgacgccg aagcgagcag gccgcacgct acggccagcc ggcgccgcag gggaccatac
     1381 gggctcatcg cccgggcgcc ctcaggggct cggctcctcg gggtggcgag cgggacgccc
     1441 gagcatcacg actccgaaga cggcgaccgc ggtcgccgtc gcggccagga tgagcaacgg
     1501 gttgatcgtg ggccccgtgg tcgtggtgac cgcctggatc gggaccgggt cgtctcggac
     1561 gacggcagca gtcgtcgggg tcgatgccgt cggggtcgat gccggcagac ccggcggcgg
     1621 cgaggtcgcg ctgagccgcc cggccggccg ggccacgggc ggcgacaccg ggtgcgaaag
     1681 cggcagcgcc ggcggccctg cggtggccgg ggcgacggcg ccggccgcca cgggcgcagt
     1741 ggtcgccggg gggctcgggg ccacagtgac ggacgccttc atgtccgggt gcacggcgca
     1801 gtagtagttg taggtgccgg ttcgggtgaa ggtgtacgac cagctctggc ccttgtcgag
     1861 ctgcggcgag tccagggtcg cgggaccgcc cgagctcgtc acggtgtggg gcgcgtcgtc
     1921 ctggttggtc cacgtgatcg tgtcgcccgc ggcgacggtc acggccgcc
//
LOCUS       DASWGG010000409         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2192778, whole genome shotgun sequence.
ACCESSION   DASWGG010000409 DASWGG010000000
VERSION     DASWGG010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192778"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VF954_07595"
     CDS             complement(<1..677)
                     /locus_tag="VF954_07595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9530985.1"
                     /translation="MERRALSCIIPPRESFAAAVGHAELAEALGYDSVNCTHIAARDS
                     FTLLAGLAMRTERIALGTAVAPIYHRSPASMAQTAATVDDLSGGRFRLGLGTGHRVTM
                     GGWHGQEIGHPLAEMREYLAIVRAVLGGEPPPDSRRWRSTFAFNGFTPRAGLPIYLAG
                     LSPGMLHLAGELADGVVLWACPPGYVRDVVVPEVARGRRQAGQDLAGFTIMAAVPAAV
                     GADRGPL"
     gene            complement(677..1516)
                     /locus_tag="VF954_07600"
     CDS             complement(677..1516)
                     /locus_tag="VF954_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9530986.1"
                     /translation="MRVARVSGRLSVLVDGGAVDVESASGGRFPADPDEVMPQWDELL
                     HWAAGYGGGEVVPYMPGALGAPLLRPAQVFGIGLNYRDHAAESGVGVPAAPAVFTKFR
                     TCLTGPYDAVRLPSGQVDWEVELVVVIGRRAEHVAEEHAWSYVAGVTVGQDLSERSVQ
                     LAGPVPQFSLGKSFPGFGPFGPAIVTPDELADPDDLELACFLDDEVLQKGRTRDMVFS
                     VPELVARLSAVCPLLPGDLIFTGTPPGVGMARRPPRFLTAGTTLVSTVENVGELRNLL
                     VAG"
     gene            complement(1535..>2200)
                     /locus_tag="VF954_07605"
     CDS             complement(1535..>2200)
                     /locus_tag="VF954_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9530987.1"
                     /translation="LRVRDPNSALQVSVQVSPRITEAAAKQEPTNGPGRPDRPNGRAP
                     AVEREGPVQPRKLGHVVIGSVDRAATQRFFVDGLGFKISDEVPGLASFMRCSTDHHNL
                     LVQQAPLNFLHHTAWEVDDVDEVGRGATKMLEGHPERHVWGLGRHHIGSNFFWYLRDP
                     AGNFSEYYSDLDCIISDGLWKPSVVEGARGLYNWGPPPPPSFIQPEDLAELMTASHGP
                     GKN"
BASE COUNT          330 a          793 c          803 g          274 t
ORIGIN      
        1 aacagggggc cacggtcggc gccgaccgcc gccggcacgg cggccatgat ggtgaagccg
       61 gccaggtcct ggcccgcctg gcgccggccc cgggcgacct cggggacgac gacgtcgcgc
      121 acgtaaccgg gcggacaggc ccacagcacc actccgtcgg ccagctcgcc ggcgaggtgg
      181 agcatccccg gggacagccc ggccaggtag atcggaagcc cggcgcgggg ggtgaacccg
      241 ttgaaggcga aggtggagcg ccagcgacgg ctgtccggcg ggggctcgcc gcccagcacg
      301 gcccgcacga tggccaggta ctcgcgcatc tcggccaggg ggtggccgat ctcctggccg
      361 tgccagccgc ccatggtgac ccggtgcccg gtgcccagcc cgaggcggaa ccggccgccg
      421 gacaggtcgt ccacggtggc ggctgtctgg gccatggacg ccggtgagcg gtggtagatc
      481 ggcgccacgg ccgtaccgag ggcgatccgt tcggtccgca tggccagccc ggccagcagg
      541 gtgaaggaat cgcgggccgc gatgtgcgtg cagttgaccg agtcgtagcc gagcgcctcg
      601 gccagttcgg catggccgac cgcggcggcg aacgactccc ggggcgggat gatgcagctc
      661 agggcgcggc gttccatcac cctgccacca ggaggttgcg gagctcgccg acgttctcga
      721 cggtgctgac cagcgtcgtg ccggcggtca ggaagcgggg cggccggcgg gccatgccca
      781 cgccgggcgg cgtgccggtg aagatcaggt cgccgggcag cagcgggcag acggcggaga
      841 gccgggccac cagctcgggc acggagaaga ccatgtcgcg ggtgcggccc ttctggagca
      901 cctcgtcatc gaggaaacag gccagctcca gatcgtcggg atcagccagc tcgtccggcg
      961 tcacgatggc cggcccgaac gggccgaacc ccgggaagga cttgcccagc gagaactgcg
     1021 gcaccgggcc ggccagctgc accgaccgct ccgacaggtc ctgtcccacg gtcacgccgg
     1081 ccacgtacga ccaggcgtgc tcctcggcga cgtgctccgc ccgccgcccg atgaccacga
     1141 ccagctcgac ctcccagtcc acctgcccgc tgggaagccg cacggcgtcg tagggcccgg
     1201 tcagacaggt gcggaacttg gtgaacaccg ccggggcggc cggcacgccg acaccggact
     1261 cggccgcgtg gtcccggtag ttgagcccga tgccgaagac ctgggccggc cgcaacagag
     1321 gggcaccaag ggcgcccggc atgtagggca ccacttcgcc gccgccgtag ccagcggccc
     1381 aatgcagcaa ctcgtcccac tgcggcatca cttcgtcggg gtcggcgggg aaccgaccgc
     1441 cgctggccga ctcgacgtcg accgccccgc cgtccacgag gacggagagc cgtccggaca
     1501 ccctggccac acgcatgcct gctcctcttc cggatcagtt cttgccggga ccgtgcgagg
     1561 cggtcatcag ctcggcgagg tcctcgggct ggatgaacga gggcggcggc ggcgggcccc
     1621 agttgtagag accccgcgcc ccctcgacga ccgacggctt ccacagcccg tcggagatga
     1681 tgcagtccag gtcgctgtag tactcggaga agttgccggc cggatcccgc aggtaccaga
     1741 agaagttcga gccgatgtgg tgccggccca ggccccagac gtggcgctcg ggatggccct
     1801 cgagcatctt ggtggccccc cggccgacct cgtcgacgtc gtcgacctcc caggcggtgt
     1861 ggtgcagaaa gttgagcggc gcctgctgca ccagcaggtt gtggtggtcg gtggagcagc
     1921 gcatgaagct ggccagcccg gggacctcgt cgctgatctt gaaccccagc ccatcgacga
     1981 agaaccgctg ggtggcggcc cggtcgaccg agccgatgac cacgtgcccg agcttgcggg
     2041 gctgcaccgg gccctcccgc tccaccgccg gcgcccggcc gttgggccgg tccggccgcc
     2101 caggcccgtt cgtcggctcc tgcttggcgg ccgcctcggt gatccgcggg ctgacctgca
     2161 cgctgacctg caacgccgag ttcggatccc gcacccgcag
//
LOCUS       DASWGG010000410         3766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_589486, whole genome shotgun sequence.
ACCESSION   DASWGG010000410 DASWGG010000000
VERSION     DASWGG010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589486"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VF954_07610"
     CDS             complement(<1..795)
                     /locus_tag="VF954_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9530988.1"
                     /translation="MTPSDVGLVAASGLVAGAMNAVAGGGTLVAFPALLATGMSARTA
                     NIVSTLGLVPGYGGGTMAYRRELEGQAGRARALAAASALGGVVGAVMLLVTSDRSFRL
                     VVPYLILASCALLVAQPWVGRRVAERQRARQADRSEIGPAVQAGVFAGAVYGSYFGAG
                     LGVLLLGVLGILLADGMQRLNALKGLLSLVINAVAVVVFVVGGHVAWWYAAILAVTAW
                     AGGILGVRFARRLSATAMRSGVVALGVVVAGAQSGRSSRKPRAPSRY"
     gene            911..2002
                     /locus_tag="VF954_07615"
     CDS             911..2002
                     /locus_tag="VF954_07615"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEX9530989.1"
                     /translation="MSNHPFPRRARRVAGAAGLTVGLAAGGYGVASAASGTTTTTPPA
                     GASASGGGSTSGTGAPGAAALPSGRPGRSGFPGGAGRKAFGRGGGTITAIAANAITVA
                     GPGGEKHQVLTDSSTTYHRDGQAAQRSDLAVGERVAVRPVRPATPPAAGTLPASSPIT
                     AADVDIISPSMQGTVDTVSGSTITITDGQGFQRTIHTSGGTTYDLGGQPASAGDVKAG
                     ERVFALGTVDADKTDLDATKISVILPRAAGIVQSVAGGTITLTEPGGATQKVTTTPST
                     VYRSNGATASASDVKVGDFLVATGQKQADGSIVATTVTFGSKPQPPGGPGPGGGWGPG
                     GPGRFGPRGAGPDGPPDATPGAPPGTATA"
     gene            complement(2030..3286)
                     /locus_tag="VF954_07620"
     CDS             complement(2030..3286)
                     /locus_tag="VF954_07620"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9530990.1"
                     /translation="MPVPRRLRPAHLAAVIAIFVVLGGGAVAMGWVGPPSGSAPPAGT
                     AGGPAAKVAAVHLTVNPADGATGVTLDAPVDVAVSDGHLLSVKVTARSGAATAAALTT
                     PGPQPGNGPASLPGALGAGSWTSSAPLEPAATYDVAATVADGTGRTSQLGTTFSTLSP
                     QKVLKVSISPLNGQTVGIGMPLALYLSQPVTDHSGFLARAQVTTTPPVAGEWHWFSDT
                     EVHYRPQTYWPSGTKVTFDGLLAGWDAGGGTWSVTPRHMAFTVGAAHVSTVDATTHQM
                     TVTDAGQVVKVLPVSTGRDKYPTDSGVHVVSEKVPTIVMDSATVGIPRASPDGYYETV
                     QWDVRISNSGEFVHAAPWSVSDQGHQNVSHGCVNLSVADGQWFFNFSNPGDIVTVAGT
                     PVPLAPTNGFGDWNVPWDQWANQQVQ"
     gene            complement(3317..>3766)
                     /locus_tag="VF954_07625"
     CDS             complement(3317..>3766)
                     /locus_tag="VF954_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9530991.1"
                     /translation="ADHHKPPWWEIPGGGMDRGESSAAAALRELREETGVRDGVRIGP
                     CVWVQHAEFDFGGYHFDQQEFIHVAWCDPNAAGRPAAWRPEALEPLEAVAFQGMRWWA
                     LDELLPSQEPVVPARLREFLPALVAGNVPDEPIDIAADAAGDQHPRL"
BASE COUNT          573 a         1397 c         1329 g          467 t
ORIGIN      
        1 gtacctggag ggggcgcggg gcttgcgcga tgacctcccc gactgagcgc ccgccacgac
       61 gacgccgaga gcgaccaccc cggagcgcat agcagtggcc gacagcctgc gggcgaagcg
      121 cacgccgagg atccccccgg cccacgcggt cacggccagg atggccgcgt accaccaggc
      181 cacgtgcccg cccaccacga acaccaccac ggcgacggca ttgatgacaa gggacaacaa
      241 gcctttgagg gcgttgagcc gctgcatgcc gtccgccagg aggatgccga gcaccccgag
      301 caggagcacg ccgagaccgg cgccgaagta cgagccgtag accgcacccg cgaacacgcc
      361 ggcctgcacg gccgggccaa tctccgatcg gtccgcctga cgggcccgct ggcgttcggc
      421 gacccgccgc ccgacccagg gctgggccac gaggagggcg cacgaggcga ggatcaagta
      481 gggcaccacc agccggaagg agcggtcgga ggtgaccagc aacatgaccg ccccgaccac
      541 gcccccgagc gcgctggccg cggcgagggc ccgggcccgc ccggcctggc cctcgagctc
      601 tctgcggtac gccatcgtcc cgccgccata ccccggaacg agaccgaggg tggacacgat
      661 gttggccgtg cgggccgaca tgcccgtcgc cagcagggcc gggaaggcga ccagcgtgcc
      721 ccctccggcc acggcgttca tggccccggc gacgagcccc gaggcggcga ccaggccgac
      781 gtccgagggc gtcatccggc catcctggca cgcccgcgag ccccaccctt ctacgccggc
      841 attccgcgcg ttgtcagccc attctcagct tggccgccca cactctcgtc agatgcgaca
      901 ggagggtccc atgagcaacc atccgttccc ccgccgggca cgacgggtgg ccggcgccgc
      961 cggcctcacg gtcggcctgg ccgccggcgg ttacggcgtg gcgtcggcag ccagcggtac
     1021 gaccaccacg acgccgccgg ccggggcgtc ggcctccggt ggcggctcca ccagcggcac
     1081 gggtgctcct ggagcagctg ccttgccttc cggccgcccc ggccgaagcg gctttccggg
     1141 cggggccggg cggaaagcat tcggccgggg cggcgggacc atcacggcca tcgccgccaa
     1201 cgccatcacc gtcgccggcc cgggcgggga gaaacaccag gtgctgaccg actcctccac
     1261 gacctaccac cgtgacgggc aagcggctca gcgctccgat ctggcagtcg gcgaacgcgt
     1321 ggccgtccgc ccggtccgcc ccgcgacccc tccggcggcc ggcaccttgc ccgcgtcgag
     1381 ccccatcacc gccgccgatg tggacatcat ctcgccgagc atgcagggca ccgtcgacac
     1441 ggtgagcgga tcgacgatca ccatcaccga cgggcagggg ttccagcgga cgatccatac
     1501 ctcgggcgga acgacctatg acctcggcgg ccagccggca tcggcaggcg acgtcaaggc
     1561 gggcgagcga gtattcgccc tcggcaccgt ggacgccgac aagaccgacc tggacgcgac
     1621 caagatctcg gtgatcctcc cccgggccgc agggatcgta cagtccgtgg caggcggcac
     1681 catcaccctc accgagcccg gtggcgccac tcagaaggtg acgaccacgc cctcgaccgt
     1741 gtaccgctcc aacggcgcca ccgcgtcggc gagtgacgtc aaagtgggtg acttcctggt
     1801 cgcgacgggc cagaagcaag ccgacgggtc gatcgtggcc acgaccgtga cgttcgggtc
     1861 caagccgcag ccgcccggcg gccctggacc gggaggcgga tgggggcccg gcggcccggg
     1921 ccggttcggg cccagagggg ccggccccga tggtccgccc gatgccacgc ccggcgcacc
     1981 tcccggcacc gcaacggcgt aggcgcacga acgacgggtg tagttcgacc tactggacct
     2041 gctggttggc ccactggtcc cagggcacgt tccagtcgcc gaacccgttg gtcggcgcga
     2101 gcgggaccgg cgtgccagcc acagtgacga tgtcgcccgg gttggagaag ttgaagaacc
     2161 actgcccgtc agcgacggag aggttgacgc agccatggct gacgttctgg tggccctggt
     2221 ccgacaccga ccacggggcg gcgtggacga actctccgga gttggatatg cggacgtccc
     2281 actgcacggt ctcgtagtac ccgtccggcg aggcccgtgg gatccctacg gtggccgagt
     2341 ccatcacgat ggtcggcact ttctccgaga ccacgtgcac gccgctgtcc gtcgggtact
     2401 tgtcccggcc ggtggacacc gggaggacct tgacgacctg gcccgcgtcg gtcacggtca
     2461 tctggtgggt cgtggcgtcg accgtcgaga cgtgggccgc cccgacggtg aaagccatgt
     2521 ggcgcggcgt cacggaccac gtgccgcccc ctgcgtccca tccggccagc agcccgtcga
     2581 aggtgacctt cgtcccgctc ggccagtagg tctggggccg gtagtggacc tcggtgtcgg
     2641 agaaccagtg ccactcgccc gctaccggcg gggtggtggt cacctgcgcc cgggccagga
     2701 acccggagtg gtccgtcacc ggctgcgaaa ggtaaagagc cagcggcatg ccgattccga
     2761 cggtctgccc gttgagcggc gagatgctca ccttgagcac cttctggggg ctgagggtgg
     2821 agaaggtcgt cccgagctgt gaggtgcggc ccgtgccgtc ggcgaccgtc gccgccacgt
     2881 cgtaggtcgc ggctggctcc agcggggccg acgaggtcca gctcccggcg ccgagcgccc
     2941 cgggcagcga cgccggcccg ttgcccggtt gcggcccggg ggtggtcagc gccgccgccg
     3001 tggccgctcc agacctcgcc gtcaccttga cggagagcag gtgaccgtcg ctgacggcga
     3061 catcgacggg cgcgtcgagg gtcacacccg tggcgccgtc cgccgggttg acggtgagat
     3121 ggacagccgc caccttggct gccggaccgc cggcggtccc cgcgggcggc gcggacccgc
     3181 tcggggggcc gacccatccc attgccaccg cgccgccacc caggacgacg aagatcgcaa
     3241 tgacagccgc cagatgggct gggcgcagcc gccggggcac gggcaccgcc tctgtgatga
     3301 tggacagccg agtgtactag aggcgagggt gctgatcgcc ggcggcgtcg gccgcgatgt
     3361 ctatcggctc gtcgggcacg ttcccggcga ccagagcggg gaggaactcc cgcaatcggg
     3421 ccggaaccac cggctcctgg gaaggcagca gctcgtcgag cgcccaccag cgcatgccct
     3481 ggaaggcgac cgcctccagc ggctcgagcg cctccggtcg ccacgcggcc ggccggccgg
     3541 ctgcgttggg gtcgcaccac gccacgtgga tgaactcctg ctggtcgaag tgatagccgc
     3601 cgaagtcgaa ctccgcgtgc tggacccaca cgcacgggcc gatgcgcaca ccgtcacgga
     3661 cgccggtctc ctcccgcagc tcccgcaggg ctgcggccgc gctggactcg ccccggtcca
     3721 tgcctccccc ggggatctcc caccacggcg gcttgtggtg gtcggc
//
LOCUS       DASWGG010000411         1632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_256953, whole genome shotgun sequence.
ACCESSION   DASWGG010000411 DASWGG010000000
VERSION     DASWGG010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256953"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..633)
                     /locus_tag="VF954_07630"
     CDS             complement(151..633)
                     /locus_tag="VF954_07630"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX9530992.1"
                     /translation="MTTIRYTTVPSPVGELLLVAGAAGLRGLYLDRPAKDVEPSWRRD
                     PGRFAEERAQLKAYFGRELKDFAVALDLDGTPFQLEVWTALQTIPYGSTFGYGQLAVQ
                     IGRSTAARAVGGATGRNPVSIIVPCHRLVGVGGRLTGYAYGLGRKRALLDLEAGVLQQ
                     "
     gene            complement(656..>1632)
                     /locus_tag="VF954_07635"
     CDS             complement(656..>1632)
                     /locus_tag="VF954_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX9530993.1"
                     /translation="RQFNASVHEVFATTPTALRHRRGPAAGSRELSLRLPYRQPMDTR
                     QTLDFLGRRAIPGVEAYDGERYQRSLALPFGAGVVTLRPATSHVRCELRLDDLRDLQA
                     AVQRARRLLDLDADPVGVDAHLGAEVALAPMVKKLPGLRVPGSVNGPELAVRAVLGQQ
                     VSLAGARTLASRLVARYGRRLPAPAGEVTHAFPSADALAGADPATFGLPAARGRALHH
                     LAEALASGRVELHPGADRAATSVGLSRLPGIGPWTAGYVAMRALGDPDVFLASDLGAR
                     RGAAALGLPDAPRALADAARAWSPWRSYAALLLWSAPAPGTPDPRRRS"
BASE COUNT          219 a          576 c          642 g          195 t
ORIGIN      
        1 tggcgtgttc gctttcgggg aggcgcgctt ctttggttcg gcggccggcc gggcggggtg
       61 gtaccggctc atctcggcgc ggcccgaagg cgacggctac tgggtggcgg gggagcagtg
      121 agcggggcgt gaggccgaac cccgagcccg tcactgctgg agcacgcctg cctcgaggtc
      181 gaggagggcg cgcttgcgcc ccaggccgta ggcgtagccg gtgagccgac cgccgacgcc
      241 caccagccgg tggcagggca cgatgatgga caccgggttg cggcccgtgg cgccgcccac
      301 ggctcgcgcc gcggtcgatc ggccgatctg gaccgccaac tggccgtagc cgaacgtgct
      361 gccgtacggg atggtctgga gggccgtcca tacctcgagc tggaagggcg tcccatcgag
      421 atccagggcg acggcaaagt ccttcaactc ccgcccgaag taggccttca gctgggctcg
      481 ctcctcggcg aagcggcctg ggtcccgacg ccaggatggt tcgacgtcct tcgcagggcg
      541 gtccaggtac aggcctcgaa ggccggccgc gcccgccacg agcagcaact cgccgaccgg
      601 gctcggcacg gtcgtgtacc ggatggttgt catggtctct ccctggtcac gaagttcacg
      661 aacgtcgtcg ggggtccggg gtaccgggcg ccggcgccga ccagaggagc agcgcggcat
      721 aggaccgcca cgggctccac gcccgggcgg catccgcgag cgcccggggc gcgtcgggca
      781 ggccgagggc ggccgcgccc cgccgggccc cgagatcgct cgccaggaac acgtcggggt
      841 cgcccagcgc ccgcatggcg acgtagcccg ccgtccatgg gccgatgccg ggcagccggc
      901 tgaggccgac cgaggtagcg gcccggtcgg cgccggggtg gagctcgact cgcccagagg
      961 ccagggcctc ggcgaggtgg tggagggctc ggccccgcgc cgctggcagg ccgaaggtcg
     1021 ccggatcggc acccgccagc gcgtcggccg aggggaacgc gtgggtcacc tcaccggccg
     1081 gcgccggcaa cctccgcccg taccgcgcca ccagccggga ggcgagggta cgggcccccg
     1141 cgagtgagac ctgctggccc agcacggccc ggactgccag ctccgggccg ttcaccgagc
     1201 cgggcacccg caggcccggc agcttcttca ccatgggcgc cagggccacc tcggcgccga
     1261 ggtgggcgtc gacgccgacc ggatcggcgt cgaggtcgag gaggcgccgg gcccgctgga
     1321 ccgcggcctg gaggtcgcgc aggtcgtcga gcctcagctc gcaccggacg tggctcgtgg
     1381 ccggccgcag ggtgacgacg ccggcgccga acggcagggc gaggctccgc tggtagcgct
     1441 caccgtcata ggcctcgacc ccggggatgg cgcgccggcc caggaagtcg agggtctggc
     1501 gggtgtccat cggctggcgg tagggaaggc gcagcgaaag ctcccgggag ccggccgcgg
     1561 gccctctccg atggcgcaag gccgtcggtg tcgtcgcaaa cacctcgtgc acggaggcgt
     1621 tgaactggcg ga
//
LOCUS       DASWGG010000412         3463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2197224, whole genome shotgun sequence.
ACCESSION   DASWGG010000412 DASWGG010000000
VERSION     DASWGG010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2197224"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VF954_07640"
     CDS             <1..606
                     /locus_tag="VF954_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530994.1"
                     /translation="VRGQEAHVQAHGRTLTDEATFAGLVALWRADLAAIRDRAGRVGP
                     FLYPNFFAVAAFRVAVLLRRRGHAILPRLITVVVTTLAQTELDPEAHVGPGFQIVHPV
                     GSGIGPGVVAGRNLTLYQGALLAAPSTRRGGFPHVGDDVVLYAKSSVIGDVSIGDGAV
                     IGAHALVLHDVGAGTTVVAPEAAAVGLQEPAPEPPPATERQ"
     gene            603..1355
                     /locus_tag="VF954_07645"
     CDS             603..1355
                     /locus_tag="VF954_07645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEX9530995.1"
                     /translation="MSGLGARLRNLDQRRRYLTFKARTAGDPELGERRDVFRHCFKAL
                     AFNGIEGHYAEFGCFGGRTFRLAYSASRLARYPTHLWAFDSFMGLPPPEDQRDLHPRW
                     VPGSMATGEARFRAVCARHGIPPSDYTVVAGYYARSLAAGAPGQRPEVVSFAYVDCDL
                     YSSTVDVLRFLEPRLRAGSILVFDDYYCYGADGPSGERAAMLEVFPESGDTALLPYHP
                     VGWHGMSFIVERRAAGTERTATGWGRSAHLRP"
     gene            1426..2862
                     /locus_tag="VF954_07650"
     CDS             1426..2862
                     /locus_tag="VF954_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX9530996.1"
                     /translation="MTRAVTGWRVGIAAGAQLAGRVVGLALGLGVAATLGRGLGTAHF
                     GDLMTALLLLSLVAPLLDFGLGSIAVREMAAEPQSRAQIAGAVILVRLVAGVALALVA
                     MAGAVALFHTWDQRWAAVGALLSLPLSALGTYQAVAQARLRPELISAMVLTQSTVWLA
                     AVVVLTQLHAPLAAYGLGYTVSVAAQGGVQWWLCHGLSKIEFRNAGVRARRLYSMSWP
                     LGLTFIFTTTYYRMDSILLYRIGGSAATGRYTAAYRFLDSLQIVPNTMVSVLLPVLSS
                     LWGDPLRRPRAQRLLSSVLTVGLAAVLPLVVGGMILSGPIVTVIYGPAFRGSARLLTE
                     LLPAFVFIFFGYVTTSLLTATRQLRALSVIALVGAVLNVAANLVLIPRFGADAAAWTT
                     VATEGLVVALGGLVAFRGVGMRLPVNRWLRIGAAGAALAGALLVVRRLPLPVAILLGG
                     GVYLVAVVVLRAVDITELRAMLRRGMDA"
     gene            complement(2819..>3463)
                     /locus_tag="VF954_07655"
     CDS             complement(2819..>3463)
                     /locus_tag="VF954_07655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9530997.1"
                     /translation="VPNGVPDLGRAAPPSPRRSLVAALVGRVDDHKRPYDFLTALRWA
                     RHQGADVTGLVLGDGPLLEEVRASVLPEERDLVWFAGFVEDPAEALLDVDVLLLMSDT
                     EGGLPLAAMEAASLSRGTVARATVEGVELLGGGAFVLPPGASPRDFGSALVALAQDRA
                     LRTRLGRAARTAFERGFSAEAAADRLAEVYEAAGRGPRARLRRPCPAAAWLAAR"
BASE COUNT          410 a         1274 c         1261 g          518 t
ORIGIN      
        1 gttcggggac aggaggcaca cgtgcaggcc cacgggcgga ccctaacgga cgaggccacc
       61 ttcgccggcc tggtggcgct gtggcgcgcc gacctggcgg ccatccggga ccgcgccggc
      121 cgcgtggggc cgttcctgta cccgaacttc ttcgccgtgg ccgccttccg ggtggccgtg
      181 ctgctgcgcc gccggggcca cgccatcctg ccccgcctca tcactgtggt ggtcacgacg
      241 ctcgcccaga ccgagctgga ccccgaggcc cacgtgggtc ccggcttcca gatcgtgcac
      301 cccgtggggt ccggcatcgg gccgggcgtg gtggcgggcc gcaacctgac gctgtaccag
      361 ggggccctcc tggccgcgcc gtcgacgcgc cgaggcgggt tcccgcacgt gggcgatgac
      421 gtggtgctct acgccaagtc gtccgtgata ggcgacgtct cgatcgggga cggggcggtc
      481 atcggggccc acgccctggt gctgcacgac gtgggcgccg gcacgacggt ggtggcgcct
      541 gaggccgccg ccgtcggcct ccaggagcca gcgcccgagc cgccacccgc gacggagcgc
      601 caatgagcgg tctcggggcc aggctccgca acctcgacca gcgccgccgg tacctgacgt
      661 tcaaggcccg gaccgccggg gaccccgagc tcggcgagcg gcgggacgtc ttccgtcact
      721 gcttcaaggc gctggcgttc aacgggatcg aagggcacta cgcggagttc ggatgcttcg
      781 gcggccgcac cttccggctg gcgtactccg catcccggct ggcccgctac cccacccacc
      841 tgtgggcctt cgactcgttc atggggctgc cgccgccgga ggaccagcgc gacctgcacc
      901 cgcgatgggt gcccgggtcc atggcgacgg gagaggcccg ctttcgcgcc gtctgcgcac
      961 gccacgggat cccgccgtcc gactacacgg tcgtagctgg gtactacgca cggtccctcg
     1021 ccgccggcgc gcccgggcag aggcccgagg tcgtctcctt cgcgtacgtc gactgcgacc
     1081 tgtactcgtc gacggtggac gtcctgcgct tcctcgagcc caggctgcga gctggcagca
     1141 tcctcgtctt cgacgactac tactgctacg gcgccgacgg gccctccggc gagcgggccg
     1201 ccatgctgga ggtctttccc gagagcgggg acactgccct cctgccctac catcccgtgg
     1261 gctggcacgg catgtcgttc atcgtcgagc gccgcgccgc cggcacggaa cgtaccgcga
     1321 cgggatgggg ccgcagtgca cacctacgac catgaggccc ccgttgaggg cgccacggcc
     1381 acgcttctga gcgagcccgg agcacccggc gcgggggcgg aggcaatgac ccgcgccgtg
     1441 accgggtggc gggtcggcat cgcggccggg gcgcagctgg caggcagggt cgtgggcctg
     1501 gccctaggtc tcggcgtggc ggcgacgctc ggccgcgggt tgggaacggc ccatttcggc
     1561 gacctcatga ccgcgctgct gctgctgtcg ctggtggccc cgctgctcga cttcgggctg
     1621 ggctcgatcg cggtgcgcga gatggcggcc gagccgcaga gccgggccca gatcgcgggc
     1681 gcggtgatcc tcgtgcggct cgtggccggg gtcgctctcg ccctggtggc gatggcgggc
     1741 gcggtagcgc tgttccacac ctgggaccag cggtgggcgg ccgtgggcgc cctgctgtcc
     1801 ctgcccttga gcgcgctcgg gacctatcag gcagtggccc aggcccggct gcggcccgag
     1861 ctcatctccg cgatggtgct gacccagagc accgtgtggc tggccgccgt ggtggtcctg
     1921 acgcagctgc acgcgccgct ggccgcctat ggcctcggct acaccgtgtc ggtggcggcg
     1981 caaggtggcg tgcagtggtg gctctgccat gggctgtcca agatcgagtt ccggaacgcc
     2041 ggggtgcggg cccgtcgcct gtactcgatg tcctggccac tcggcctgac cttcatcttc
     2101 acgaccacct attaccggat ggacagcatc ctgctgtacc gcatcggggg gagcgcggcc
     2161 acgggccgat acacggcggc gtaccggttc ctcgactcgc tccagatcgt gccgaacacc
     2221 atggtcagcg tgctgctccc ggtgctctcg tcgctgtggg gcgatccgct ccgccggccg
     2281 cgcgcccagc ggctgctgtc ctcggtcctc accgtcggcc tggccgccgt gctgccgctc
     2341 gtcgtgggcg ggatgatcct gtcgggcccg atcgtcaccg tcatctacgg gccggccttc
     2401 cgcggctcgg cccggctgct cacggagctg ctgccggcgt tcgtcttcat attcttcggg
     2461 tacgtcacga ccagcctgct caccgccacc cgccagctgc gggccctctc cgtcatcgcc
     2521 ctggtcggcg cggtgctcaa cgtggccgcc aacctcgtgc tcatcccgag gttcggggcg
     2581 gacgcggccg cgtggaccac ggtggcgacc gaggggctgg tcgtcgccct gggtggcctg
     2641 gtcgcgttcc gcggcgtcgg catgcgcctc cccgtcaacc gctggctgcg gattggtgcc
     2701 gcgggagccg ccctggcagg cgcgctcctc gtggtgcggc gcctgccgct tcccgtcgcg
     2761 atcctccttg gtggcggcgt ctacctggtg gcggtcgtgg tcctccgggc agtggacatc
     2821 accgagctgc gagccatgct gcggcggggc atggacgcct gagtcgggcc cgaggcccac
     2881 gccccgccgc ctcgtacacc tctgccaggc gatccgcggc cgcttcggcc gagaagccac
     2941 gctcgaacgc ggtgcgggcc gctcgtccca gccgggtccg aagggcacgg tcctgcgcca
     3001 gggcgacgag ggcggagccg aagtcgcgag gagacgcgcc tggcgggagc acgaaggcgc
     3061 ccccacccag cagctccacg ccctctaccg tggcccgggc gaccgtcccc cgggagaggg
     3121 aggcggcttc catcgccgcc aggggaaggc cgccctcggt atcggacatc agaaggagca
     3181 cgtccacgtc gagcagggcc tcggccgggt cctcgacgaa ccccgcgaac cagaccaggt
     3241 cgcgctcctc cggcaggacg gaggcgcgca cctcctcgag cagcgggccg tcgccgagaa
     3301 cgagcccggt gacgtcggcg ccctggtgcc gggcccagcg cagcgcggtc aggaagtcgt
     3361 agggcctctt gtgatcgtcg acccggccga ccagcgcggc gacgagcgat cgacgagggg
     3421 atggcggcgc ggcccgcccg aggtccggca cgccgttcgg gac
//
LOCUS       DASWGG010000413         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2198806, whole genome shotgun sequence.
ACCESSION   DASWGG010000413 DASWGG010000000
VERSION     DASWGG010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198806"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VF954_07660"
     CDS             <1..298
                     /locus_tag="VF954_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9530998.1"
                     /translation="VAGVLGVAWLAWRDRRRPGPPDRDAPDAREALEGADTEGDDTDH
                     KEDGGEGARDDGWHGTPEPALVPVGAPLDAPPTFAPGGPGREADPLGWDDPLDH"
     gene            311..1705
                     /gene="manB"
                     /locus_tag="VF954_07665"
     CDS             311..1705
                     /gene="manB"
                     /locus_tag="VF954_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442410.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX9530999.1"
                     /translation="MSTALDEIFKAYDVRGTVPDQWDSGAARRIGAAFARFAAAPRVL
                     VARDMRPSGVALVAAFAEGVMAQGVDVTDLGLASTDLIYFAAGRLDAPGAMFTASHNP
                     ARYNGIKFCLSGARPVGEDSGLAEVKALAGQDLAPDGRTGHRDELDLLEDYAAHVRSF
                     VDRDALLPLKVVADTANGMGGLVVPKVFEGLPFSLEMLYGELDGTFPNHPADPIQPEN
                     LRDLQARVLAAGADVGLAFDGDADRVFLVDDRGQAVSGSTTTAMVATAMLARHPNSVI
                     LHNIICSKAVPEIIREHGGTPVATRVGHSFIKAVMASTGAVFGGEHSAHYYFRDNYRA
                     DSGAIAALIVLEMLSRAGRPLSVFRTAFERYCDSGEINTPVADPQHVLDRVAAHFDPT
                     VYPGVILAHTDGLTVDLGDWWFNLRPSNTEPLLRLNLEAGDRGSCAAHLAEVLDLISG
                     AAAPPDPSATDEER"
     gene            1708..1953
                     /locus_tag="VF954_07670"
     CDS             1708..1953
                     /locus_tag="VF954_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX9531000.1"
                     /translation="MALDPLLLDILACPEDKGPLLYFQDEDCLYNPRLKRRYAIRDDI
                     PIMLIDEAETVDDAEHDRLMSRAAAEGIKPTFDPGAP"
     gene            1950..>2328
                     /locus_tag="VF954_07675"
     CDS             1950..>2328
                     /locus_tag="VF954_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX9531001.1"
                     /translation="MSQAATGVLDTDGMFEASSGLPEQVQAALGSARGLDRLPDREMV
                     ENVVMLGMGGSGIAGDLLLAVAAPFMAMPVVVLKSYTLPAFVGEGSLVFAVSYSGNTE
                     ETVEAATEAAAQGARMVIVTTGGE"
BASE COUNT          328 a          869 c          811 g          320 t
ORIGIN      
        1 cgtcgccggt gtgctcggcg tcgcctggct ggcgtggcgc gaccggcgcc gcccgggccc
       61 gccggaccgc gacgcgccgg acgcccggga agcgctcgag ggcgccgaca ccgaaggcga
      121 cgacaccgac cacaaagagg acggcgggga aggcgcccgc gacgacgggt ggcacgggac
      181 ccccgagccc gccctcgtgc cggtcggcgc gcccctcgac gcgccaccga cattcgcgcc
      241 cggcggcccc gggcgcgagg cagacccgct cgggtgggac gatcccctcg accactagtg
      301 tcgggccggc atgtcgacgg ccctcgacga gatcttcaag gcctacgacg tgcgtggcac
      361 cgtcccggac cagtgggact ccggcgccgc ccgccgcatc ggggcggcct tcgcccggtt
      421 cgcggccgct ccacgggtcc tggtcgcccg cgacatgcgg ccctcgggcg tcgccctggt
      481 ggcggctttc gccgagggag tcatggccca gggcgtggac gtcaccgacc tggggctggc
      541 gtcgaccgac ctgatctact tcgcggccgg ccggctcgac gcgccgggcg ccatgttcac
      601 cgcctcgcac aacccggcgc ggtacaacgg catcaagttc tgcctgtcgg gggccaggcc
      661 cgtgggcgag gacagcggcc tggccgaggt caaggcgctg gccggccagg acctggcgcc
      721 ggacgggcgg acgggccacc gcgacgagct cgacctgctc gaggactacg ccgcccacgt
      781 gcgctccttc gtggaccgtg acgcgctgct cccgctgaag gtggtggccg acacggccaa
      841 cggcatgggc gggctggtgg tcccgaaggt cttcgaaggg ctgccgttct cgctggagat
      901 gctctacgga gagctggacg gcaccttccc caaccacccc gccgatccga tccagcccga
      961 gaacctgcgc gacctccagg cccgggtgct ggccgccgga gccgacgtgg gcctggcctt
     1021 cgacggggat gccgaccggg tcttcctcgt ggacgaccgg ggccaggcgg tgtcgggctc
     1081 gacgaccacg gcgatggtgg ccacggccat gctggctcgg catcccaact cggtgatcct
     1141 gcacaacatc atctgctcca aggccgtgcc cgagatcatc cgcgagcacg gcggcacgcc
     1201 ggtggccact cgcgtgggcc actcgttcat caaggccgta atggcctcga cgggagcggt
     1261 gttcgggggc gagcactcgg cccactacta cttccgcgac aactaccggg ccgacagcgg
     1321 ggccatcgcg gccctgatcg tgctcgagat gctctcgcgc gccggccggc cgctgtcggt
     1381 ctttcgcacc gcgttcgagc gatactgcga ctcgggcgag atcaacaccc cggtcgcgga
     1441 cccgcagcac gtgctggacc gggtggccgc ccatttcgac cccacggtct accccggggt
     1501 catcctggcc cacaccgacg ggctgaccgt cgatctcggc gactggtggt tcaacctccg
     1561 cccgtccaac accgagccgc tgctccgcct caacctcgag gccggcgacc gcggctcctg
     1621 cgcggcccac ctcgccgagg tcctcgatct catctcgggc gcggccgcgc cccccgaccc
     1681 atccgccacc gacgaggagc gctgaccgtg gctctggacc ccctgctgct cgacatcctg
     1741 gcctgccccg aggacaaggg gccgctgctg tacttccagg acgaggactg cctctacaac
     1801 ccgcgcctga aacggcgcta cgccattcgg gacgacatcc ccatcatgct gatcgacgag
     1861 gccgagaccg tcgacgacgc cgagcacgac cgcctgatgt caagggccgc cgcggaaggc
     1921 atcaagccca cgttcgatcc cggggcgcca tgagccaggc ggcgacgggg gtgctcgaca
     1981 cggacgggat gttcgaggcc tcgagcggtt tgcccgagca ggtccaggcg gccctcgggt
     2041 cggcgcgcgg cctcgatcgc cttcccgacc gggagatggt cgagaacgtg gtcatgctcg
     2101 gcatgggtgg cagcggcatc gccggcgacc tcctgctcgc cgtcgccgcc cccttcatgg
     2161 cgatgccggt cgtggtgctc aagtcctaca cgctgcccgc gttcgtggga gaagggtcgt
     2221 tggtcttcgc cgtctcatat tccggcaaca ccgaggagac ggtggaagcg gccaccgagg
     2281 cggccgccca gggggcccgc atggtcatcg tgaccacggg cggcgagc
//
LOCUS       DASWGG010000414         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2203918, whole genome shotgun sequence.
ACCESSION   DASWGG010000414 DASWGG010000000
VERSION     DASWGG010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2203918"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..420
                     /locus_tag="VF954_07680"
     CDS             214..420
                     /locus_tag="VF954_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531002.1"
                     /translation="MAIGQGLGYGLLGLAVAGFGAGAVLGFGPLVTVIVIGALLAMTV
                     VLAPAIVFGFGVRAAEREDRRARS"
     gene            508..1617
                     /locus_tag="VF954_07685"
     CDS             508..1617
                     /locus_tag="VF954_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEX9531003.1"
                     /translation="MARAAVFVGVDQPLEIRDDVEVEAPHAGEIKVRMAASGVCHTDL
                     SVQNGTIFQMAPAVLGHEGAGVVEAVGEGVTSVKPGDHIVVSWVPQCGECFYCTRDQG
                     YLCERAGAALASGGLLDGTTRFTSGGGSLFQMAASGTFSEVSIIPATGAVKIPEDFDL
                     KLAALIGCGVLTGVGAAMNTADIRPGDTVAVVGCGGVGLNVIQGARIAGAGRIIAIDM
                     VETKLQMAKEFGATDSVDASQTDPVGQVMAMTEQRGADVAFEVIGLQQTIEQTITMTR
                     RGGEAVLVGVPRMDVMVQVPAFLGLVLQDKAIKGCWYGSSNVQKDVPKLIGLYRQGQL
                     KLDELVSRTISVDQVNEAFAAMKSGEVARSVIQYQ"
BASE COUNT          287 a          721 c          791 g          304 t
ORIGIN      
        1 cggcgtggtc ctcgtcctgg cgcccggcga ggagctgctg gtcgggtcgg ccgtcgtccg
       61 aggcgcgggg gaccacgacg ccatggcccg ggccgtcctc gacgccacca accgccggct
      121 gctcgaaccg ggccggtgag cgggcctgga caggcgggac cggccgccag ccccgatccc
      181 gtgctagccc gccgggcgcg catcgctcga tgggtggcca tcgggcaagg cctcgggtac
      241 gggctcctcg ggctggcggt ggccggcttc ggcgcgggcg cggtcctcgg cttcggcccg
      301 ctcgtcaccg tgatcgtgat cggcgccctg ctggccatga ccgtcgtgct ggctccggcg
      361 atcgtgttcg gcttcggggt gcgggcggcc gagcgagagg accgccgggc ccggagctga
      421 ggccgtcttg cccggcgggc ccggcgatgg aacgatgcag gccgcgcctc acaccgcagg
      481 gcctgcgtac caggggagat ggcttgaatg gctagagcag cagttttcgt cggcgtcgac
      541 caaccgctcg agatccgtga cgacgtggag gtggaggcgc cccacgccgg cgagatcaag
      601 gtgcgcatgg ccgcctcggg cgtgtgccac accgacctgt ccgtccagaa cggcaccatc
      661 ttccagatgg cgcccgccgt gctgggccac gagggcgccg gggtcgtgga agcggtcggc
      721 gagggcgtca cgtcggtgaa gccgggcgac cacatcgtgg tgtcgtgggt gccgcagtgc
      781 ggcgagtgct tctactgcac gcgtgaccag ggctacctct gcgagcgggc cggggccgcg
      841 ctggccagcg gcggcctgct cgacggcacg acgcgcttca cgtcaggcgg tgggtcgctg
      901 ttccagatgg cggcgtcggg cacgttctcc gaggtctcga tcatcccggc caccggcgcg
      961 gtcaagatcc ccgaggactt cgacctgaag ctggctgccc tcataggttg cggcgtgctc
     1021 accggggtcg gcgcagccat gaacacggcc gacatccgcc cgggagacac cgtcgcggtg
     1081 gtcggatgcg gcggcgtcgg gctcaacgtg atccaggggg ctcgcatcgc cggggcgggc
     1141 cggatcatcg ccatcgacat ggtggagacg aagctgcaga tggccaagga gttcggcgcg
     1201 accgacagtg tcgacgccag ccagaccgac ccggtcgggc aggtcatggc catgaccgag
     1261 cagcgcggcg ccgacgtggc cttcgaggtc atcgggttgc agcagaccat cgagcagacc
     1321 atcaccatga cccgtcgcgg cggcgaggcc gtgctggtcg gagtgccgcg catggacgtc
     1381 atggtgcagg tgcccgcgtt cctcgggctg gtgctccagg acaaggcgat caagggctgc
     1441 tggtacggct cctcgaacgt gcagaaggac gttcccaagc tcatcgggct ctaccggcag
     1501 ggccagctca agctggacga gctcgtgtcg cgcacgatct cggtggacca ggtgaacgag
     1561 gccttcgccg ccatgaagtc gggcgaggtg gcccggtcgg tcatccagta ccagtgaccg
     1621 gcccgccgcc gagggtggcg ccggcgtggc ctgggctcag atcccgggag gctggagcct
     1681 cccgtagcgg cggcggcggg cgcgaaccac gaggagaccg acgcccgggc tccgagccgg
     1741 cgccggccgc accggctccg gcgggcgccg cgtgccgcgt tctgggtgcg actggggacg
     1801 cttcacgtcc tgcttttcgc catccgggca gggcgttcct tcccgatgcc gcgaagtgca
     1861 caatacgtcg ccgccccttg acagcctttg acgaaggccc cagcccggtg cggccgtcga
     1921 acggggcccg aggttgttac cttggggtaa tgaaggcgtc atcttcgccc gccccgtcgg
     1981 gcccgggcgc cgagaccggg ccatcgagct ccccccggct gccagccgcc cggcggcgcc
     2041 ggcaactgct ggacaccgcg cacgacgcct tcgcgcagcg aggctttcac agcacgtcga
     2101 tgg
//
LOCUS       DASWGG010000415         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2205718, whole genome shotgun sequence.
ACCESSION   DASWGG010000415 DASWGG010000000
VERSION     DASWGG010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2205718"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1183
                     /locus_tag="VF954_07690"
     CDS             86..1183
                     /locus_tag="VF954_07690"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9531004.1"
                     /translation="MALRVDDVDLARDRTLVERAQAGDGAAFAELYARYFARLCRFCE
                     RRVGDAHEAEEVAQEAFTRACRALPGLAGERRFYPWLSVIAARLCVDVHRRRARSRPT
                     PAVDAGWVDGGQEDVLRAVDTALVRTALAQLSPRHQEILRLREWEDWSYERLAEHLDM
                     TMAGIEALLWRARQALRREVERLDYRSGRWAALPVVAWLVRRLAGARRALATAVESPL
                     APVVSGAVSAAVVGALALGVAAGSSGPSRPLPTIGVRSAAPAGSHEATVSPDRLPTAP
                     PAPLPHAPAPLAPTPAPTPAPSSASGAAPSAYHPVRNSSLGGYGSAGPGEESRQPVQS
                     SAAGTAVGVDPGKPATDLNADVQHVLGRSIP"
     gene            1213..1407
                     /locus_tag="VF954_07695"
     CDS             1213..1407
                     /locus_tag="VF954_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531005.1"
                     /translation="MATSLLAVSLMVAGALTGGAAASASPAAPHQYADGYWVCVANQQ
                     LDVEYCQGDPTPPIGPILVP"
BASE COUNT          193 a          569 c          574 g          210 t
ORIGIN      
        1 gagcagtcga agtgtgagcc caggaggaag gcgggctccg ggcaccgaat actgcttgca
       61 ggaaagctca ttcagtcggg ggatcatggc cctacgggtt gacgatgtcg atctggcgcg
      121 cgaccggact ctggtggagc gcgcccaggc cggtgacgga gccgccttcg ccgagctcta
      181 cgcccggtac ttcgcccgcc tgtgccggtt ctgcgagcgg cgggtgggcg atgcgcacga
      241 ggccgaggag gtggcgcaag aggcgttcac gagagcctgc cgggcgcttc ccggcctcgc
      301 aggcgagcgc cgcttctacc cctggctgtc ggtcatagcg gcgcggctct gcgtcgacgt
      361 gcaccgccgc cgcgcccgca gccgcccgac gccggccgtc gacgccggct gggtcgacgg
      421 cggccaggag gacgtgctga gggccgtgga caccgccctg gtgcgcaccg cgttggctca
      481 gctgtcgcca cgacaccagg agatcctccg cctgcgggag tgggaggact ggtcctacga
      541 gcgcctggcc gagcacctgg acatgaccat ggccgggatc gaggcgctgc tgtggcgcgc
      601 ccggcaggcg ctgcgtcgcg aggtggagcg cctggactac cggagcgggc ggtgggctgc
      661 cctgcccgtg gtcgcatggc tcgtccggcg cctcgcggga gcccgccgcg ccctggccac
      721 agccgtcgag agcccactgg cgccggtcgt aagcggcgcc gtgtcggcag ccgtggtcgg
      781 ggccctggcc ctcggtgtgg ccgccggctc gtccgggccg tcgcgtccgt tgccgacgat
      841 cggcgtgcgc agcgccgccc cggccggctc gcatgaggcc accgtctcac cggatcggct
      901 gcctacagcg ccaccggcgc ctctaccgca cgcgccggca cccctggccc cgacccccgc
      961 cccgaccccg gccccgtcgt cggcgagcgg agcggcgcct tccgcctacc acccggtccg
     1021 caacagcagc ctcggcgggt acggctccgc cgggcccggc gaggagtcgc ggcagccggt
     1081 gcagtcctcg gccgccggca cggcggttgg ggtggacccg ggcaagccgg ccaccgatct
     1141 caatgcagac gtgcagcacg tcctggggag gagcatcccg tgattcgaat ggttcgcttt
     1201 ggcatggccg gcatggccac gtcgctgctg gcggtgtccc tgatggtcgc cggcgccctg
     1261 actgggggtg cggccgcctc ggcgtcgccc gcggcgcctc accagtacgc agacggctac
     1321 tgggtctgcg tggccaacca gcagctcgac gtcgagtact gccagggcga ccccaccccg
     1381 cccatcggtc cgatcctcgt cccctagcac cccgactccc cggagggtcg ggcggcgatc
     1441 aggcgggtaa ctgcctcgtg gccgcccgat ggccccacaa tgtgctgagg taggagacgc
     1501 ccgccagggc gaggccgatg gcgagtatgc ccaggccccc ggggag
//
LOCUS       DASWGG010000416         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2463025, whole genome shotgun sequence.
ACCESSION   DASWGG010000416 DASWGG010000000
VERSION     DASWGG010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2463025"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VF954_07700"
     CDS             <1..201
                     /locus_tag="VF954_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531006.1"
                     /translation="RADRVAVVDQGTLRELGTHDELLARGGRYAALFASWAGGQAAES
                     GAVGGADAITAAAMPCAERRPS"
     gene            212..1075
                     /locus_tag="VF954_07705"
     CDS             212..1075
                     /locus_tag="VF954_07705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9531007.1"
                     /translation="MSTAEVDVILVHRDQPERCVLAARAFQAQVGVRTRLTVVDNGSP
                     APARERLAAALPGVAMVALGRNAGFGPGANAGLRAWLTDGAGDWAVVAPHDALPEPDC
                     LRRLLAAVAERPRAGLVSAEYGEANRPVIDAYLGGMLVPMPRRDGWVATEHPHGTLLA
                     CARACLEDIGLFDERYFAYVEEADLGLRAAARGWEVGMVVGALVVNPVTSSPPAVVDY
                     LQLRNTLLLIRDHFGRYPATMRGLIAVVSVVHQSLRPSARAPWFSPRARLRAVADFAR
                     GRFGPPPAALG"
     gene            1257..>1507
                     /locus_tag="VF954_07710"
     CDS             1257..>1507
                     /locus_tag="VF954_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX9531008.1"
                     /translation="MVSTELDLGRYKLGWSDKVDYAFTPKKGLSEEVVREISWWKGEP
                     QWMTDFRLKAYRHFERRPFPTWLAPSSHEIHFDDIYYYL"
BASE COUNT          202 a          544 c          542 g          219 t
ORIGIN      
        1 cgggccgacc gggtggccgt cgtcgatcag ggcaccctcc gcgagctggg cacacacgac
       61 gagctgctcg cccggggcgg gcgctacgcc gccctgttcg cctcctgggc cggcggccag
      121 gccgccgagt ccggcgccgt cggcggggcc gacgccatca ccgcggccgc catgccgtgc
      181 gccgagcgcc gcccctcgtg agccgcttcc catgagcacg gccgaggtgg acgtgatcct
      241 ggtccaccgg gaccagccgg agcgctgcgt cctggcggcc cgggccttcc aggcccaggt
      301 cggggtccgc acccggctga ccgtcgtgga caacggctcg ccagcgccgg cgcgggaacg
      361 gctggccgcc gccctgcccg gggtggccat ggtggccctg gggcgcaacg ccgggttcgg
      421 gccgggcgcc aacgccggcc tgcgggcctg gctgaccgac ggggcgggcg actgggcggt
      481 cgtagccccc cacgacgccc tccccgagcc cgactgcctg cgccggctcc tcgcggccgt
      541 ggccgagagg ccaagggccg ggctggtctc ggccgagtac ggggaggcca accgccccgt
      601 catcgacgcc tacctgggcg ggatgctggt gccgatgccg cgccgcgacg gttgggtggc
      661 gaccgagcac ccccacggca ccctgctggc ctgcgcccgg gcctgcctgg aggacatcgg
      721 cctcttcgac gagcggtact tcgcctacgt ggaggaggcc gacctgggac tgcgggccgc
      781 cgccagggga tgggaggtcg gcatggtcgt cggcgccctg gtggtcaacc cggtgacctc
      841 ctcgccgccc gccgtcgtcg actacctcca gcttcgcaac accctgctgc tgatccgcga
      901 ccacttcggg cgctatcccg ccaccatgcg cggcctcatc gccgtggtct cggtcgtcca
      961 ccagtcgctg cggccgtcgg cccgggcgcc gtggttctcg ccgcgggcgc gcctgcgggc
     1021 cgtggccgac ttcgcccggg ggcgcttcgg gcccccgccg gccgccctgg gctgaccttg
     1081 gctgatcgcc cgcggcagcc gcaccaaagt cattcttcca gcaaaaaagt cgctggggcg
     1141 acattaaagc attgaagaat ggtctggtgg ccggatgaag tgcggcggca ggattgttac
     1201 tatggccgta ggggaaatta ggccgtaacc cccgaggagt gccgaggagt gatcgcatgg
     1261 tgagcaccga actggatctg ggccgctaca agctcgggtg gtccgacaag gtcgactatg
     1321 cctttacccc caagaagggc ctgtccgagg aggtcgtgag ggagatctcg tggtggaagg
     1381 gcgagccgca gtggatgacc gacttccgac tgaaggcgta ccgccacttc gagcgccggc
     1441 ccttcccgac gtggttggct ccgagcagcc atgagatcca cttcgacgac atctactact
     1501 acctgaa
//
LOCUS       DASWGG010000417         2379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_274420, whole genome shotgun sequence.
ACCESSION   DASWGG010000417 DASWGG010000000
VERSION     DASWGG010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274420"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VF954_07715"
     CDS             <1..1138
                     /locus_tag="VF954_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX9531009.1"
                     /translation="EGEPGTFKDRAIIRANPYQVIEGLAIAALTVGAPEVFVATKGSF
                     EPERAALTRAIQEMDAAGLLDGLTVALVGGPDEYLFGEEKAMLEVIEGHDPLPRLLPP
                     FQHGLFATVTQTGWESHSAETGGIPAAANPTLVNNCETLANVPHILANGAEWFRSMGT
                     EESPGTIVCTASGDVRRATVVELEMGTPLSELVDRAGGARPGRTLKAAFSGVANAVVT
                     AASFGTGLSYEDMAGIGSGLGSAGYVFYDDSACMVEVAAVLSRFLYVESCGQCPPCKL
                     GSGQITDALEAIERGTGGDDSVGLIARALRTVTDGNRCYLPVEEQNLVASILKAFPEE
                     VSAHVVGGCPRPRELLPPKIVDIVDGKVVWDARQARKRPDWTYG"
     gene            complement(1143..1916)
                     /gene="fabI"
                     /locus_tag="VF954_07720"
     CDS             complement(1143..1916)
                     /gene="fabI"
                     /locus_tag="VF954_07720"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX9531010.1"
                     /translation="MALLEGKRILVTGVLTDDSLAFGVAELAQREGAEIVLTGAGRGL
                     SLTERTARKLPSQPDVLEMDVTVADHLDAVAADLRDRWGRVDGVLHAIGFAPEACLGG
                     DILRAGWDDVAVAMHVNAFSLKALAQAMLPLLEAGGGGSVVGLDFDARFAWPAYDWMG
                     VAKAALESVSRYLARDLGSRQVRVNLVAAGPVRTMAAKSIPGFAAFEDAWDGRAPLGW
                     DVRDPTAVAKACVALLSDWFPKTTGELIHVDGGYHAMGA"
     gene            1982..>2379
                     /locus_tag="VF954_07725"
     CDS             1982..>2379
                     /locus_tag="VF954_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX9531011.1"
                     /translation="MHVGVLGGTGPAGRGLGARLASVGVTVTIGSRSRDRAEETCRTL
                     LAAWPGHRLDVRAGDNETAAAADVVVVATPWDAAVPTVASVEPCLGGKVVISMANAIA
                     RIGKEFEPLVPPRGSVAAAVQAVAPRSMVAA"
BASE COUNT          335 a          858 c          875 g          311 t
ORIGIN      
        1 cgaaggcgag cccggcacct tcaaggaccg ggccatcatc cgggccaacc cctaccaggt
       61 gatcgaaggc ctggccatcg ccgccctcac cgtcggcgcc ccggaggtgt tcgtggccac
      121 caagggctcc ttcgagcccg agcgagccgc cctgacccgg gccatccagg agatggacgc
      181 cgccggcctg ctcgacgggc tgacggtcgc gctggtgggg gggccggacg agtacctctt
      241 cggcgaggag aaggccatgc tggaggtgat cgagggccac gacccgctgc cccgcctgct
      301 gccgccgttc cagcatgggc tgttcgccac cgtcacccag acgggatggg agagccacag
      361 cgccgagacc ggcggcatcc cagcggcggc caacccgacc ctcgtgaaca actgcgagac
      421 gctcgccaac gtgccccaca tcctggccaa cggggcggag tggttccgct cgatgggcac
      481 cgaggagtcg cccggcacca tcgtgtgcac ggcgagcggc gacgtgcgtc gggccaccgt
      541 cgtggagctg gagatgggga cccctctttc cgagctggtc gaccgcgccg gtggggcccg
      601 gccggggcgc acgctcaagg cggccttctc cggcgtggcc aacgccgtgg tgacggcggc
      661 ctcgttcggc accgggttga gctacgagga catggccggc atcggcagcg ggctcggctc
      721 ggcgggctac gtcttctacg acgactcggc ctgcatggtg gaggtggcgg ctgtgctctc
      781 gcggttcctg tacgtcgagt cgtgcggcca atgccccccc tgcaagctgg gcagcggcca
      841 gatcaccgat gccctggagg ccatcgagcg gggcacgggc ggcgacgaca gcgtcgggct
      901 catcgcccgc gccctgcgca cggtgaccga cggcaaccgg tgctacctcc cggtcgagga
      961 gcagaacctg gtcgccagca tcctgaaggc cttccccgag gaggtctcgg cgcatgtggt
     1021 aggcggctgc ccccgcccgc gggagctgct cccgcccaag atcgtcgaca tcgtcgacgg
     1081 gaaggtggtg tgggacgcca ggcaggcccg caagcgaccc gactggacct acgggtgagc
     1141 cgtcaggccc ccatggcgtg gtagcccccg tcgacgtgga tcagctcgcc ggtggtcttc
     1201 gggaaccagt ccgacaggag cgccacgcac gccttggcca ccgccgtcgg gtcgcgcacg
     1261 tcccagccca gcggcgcccg gccgtcccag gcgtcctcga aggccgcgaa gccgggtatg
     1321 gacttcgccg ccatggtccg gaccggcccc gctgccacca ggttgacgcg cacctggcgg
     1381 gacccgaggt cgcgggccag gtagcggctc accgattcca gggcggcctt ggccacgccc
     1441 atccagtcgt aggcgggcca ggcgaagcgg gcatcgaagt cgagcccgac caccgacccg
     1501 ccgcccccgg cctcgaggag cgggagcatg gcctgggcca gcgccttgag cgagaaggcg
     1561 ttcacgtgca tcgccaccgc gacgtcgtcc cacccggctc gcaggatgtc gccgccgagg
     1621 caggcctccg gcgcgaagcc gatggcgtgg agcaccccgt ccacgcggcc ccagcggtcc
     1681 cggaggtccg cggccaccgc gtccaggtgg tcggccacgg tgacgtccat ctcgagcacg
     1741 tccggctggg acgggagctt gcgggccgtg cgctcggtca gggacaaccc acgtccggct
     1801 ccggtcaaca cgatctccgc cccctcccgc tgggccagct cggccacgcc gaaagcgagc
     1861 gaatcgtcgg tgagcacccc ggtgacgagg atgcgcttgc cttcgagcag tgccatgtga
     1921 cctccgactc ccacccgccg gctgtggtta gccccggcgc gggtgtgcaa aggtaccgcc
     1981 cgtgcacgta ggcgtcttgg gaggaacagg cccggccggc cggggtctgg gcgcccgcct
     2041 ggcgtcggtc ggggtgacgg tcaccatcgg ctcgcgaagt cgggaccggg cggaggagac
     2101 ctgccggacc ctgctggccg cctggccggg ccaccggctc gacgtgcggg ccggcgacaa
     2161 cgagacggcg gcggcagccg acgtggtcgt ggtggccacg ccgtgggacg cggcggtgcc
     2221 gacggtcgca tccgtggagc cctgtctcgg cggcaaggtc gtgatctcca tggccaacgc
     2281 catcgccagg atcggcaagg agttcgagcc cctggtgccg ccgcgggggt cggtcgcggc
     2341 ggcggtgcag gcggtcgcgc cgcgctcgat ggtggcggc
//
LOCUS       DASWGG010000418         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_277003, whole genome shotgun sequence.
ACCESSION   DASWGG010000418 DASWGG010000000
VERSION     DASWGG010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277003"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..814
                     /locus_tag="VF954_07730"
     CDS             119..814
                     /locus_tag="VF954_07730"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEX9531012.1"
                     /translation="MTSELNLPAPPWRLEGDAIVAFAGVPAAERERLPGGARGLPGPA
                     LVFAISYSSSPVGPYLETVVCIPAWYRGRPGFCVTTIAVDSEPALWAGLDFWGYPKVL
                     GTMEWEADGPVRELRWKERDLVVRARAATRPVPAATIVPTVQALPDGGGAFTGLRIVG
                     RLAPARVEIEAPDGPLAGLAGRHQGTVAAGMRMSVPRPALLGSASPRTAAALAGEILL
                     PAYRPDQIGTRLA"
     gene            complement(841..>1636)
                     /locus_tag="VF954_07735"
     CDS             complement(841..>1636)
                     /locus_tag="VF954_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531013.1"
                     /translation="ARRRRLPLSRYPKEVVVNTRLTQAVRAAGGPIEQIGRDWMLHAE
                     TFARSEAHGYPHPFAGYFAGRGGVLGDVDWEVVDAVFAVFEPGVTKLFWEQGRAVHGA
                     AEGARLYWEQAADWGRDHLTGAPGLDRIVELGERIIDAAPAAGMPLFCAWRLMPRAPD
                     APARALQVLFILRELRGSIHLPALISAGIKPVEGHILNKGPEFAAFCGFQEPFPDCAH
                     LKARKDEVEEITDQRMAEIVETALSPAEAEELAELAAAALRVASAI"
BASE COUNT          219 a          592 c          586 g          239 t
ORIGIN      
        1 ctgggcgccg atagcctcgg ccttatcagc tactacgacg aaaagaacga gaaactcaaa
       61 gtggcgcatt gtgcgacaac gccgtgcttt tctggcctcg tcggcccata attgcgccat
      121 gacgagcgaa ctgaacttgc cggcaccgcc ctggcgtctc gagggtgacg ccatcgtcgc
      181 ctttgccggc gtaccggccg ccgagcggga gcggctgccc ggcggcgccc gggggctccc
      241 cggccccgcc ctggtgttcg ccatctccta ttcgagttcg cccgtcggcc cctatctgga
      301 gacggtcgtc tgcatccccg cctggtaccg cggccggccc gggttctgcg tgaccaccat
      361 cgcggtggac tccgaaccgg cgctgtgggc cgggctcgac ttctggggct acccgaaggt
      421 cctcggcacc atggaatggg aggccgacgg tccggtccgg gagctgcgat ggaaggaacg
      481 cgacctggtg gtccgcgccc gggcggcgac gaggcccgtg ccggcggcca cgatcgtccc
      541 caccgtgcag gcgctgcccg acggcggcgg tgccttcacc ggtctgcgga tcgtcggtcg
      601 actggccccg gcccgggtcg agatcgaggc cccggacggt ccgttggccg gcctggcggg
      661 ccgccaccag ggcaccgtgg cggcggggat gcggatgtcc gtgccgcggc cggcgctcct
      721 cggctccgcc tcgccccgca ccgcggcggc cctggccggc gagatcctgc tgcccgccta
      781 ccgcccggac cagatcggca cccgcctcgc ctagcgctcg gagggcggcc cggtaccgca
      841 ctagatggcc gaggcgaccc tgagcgccgc ggcggccagc tcggccagct cctcggcctc
      901 ggccggcgag agtgcggtct cgacgatctc ggccatgcgc tggtccgtga tctcctccac
      961 ctcgtccttg cgggccttca ggtgggcgca gtccgggaac ggctcctgga agccgcagaa
     1021 ggcggcgaac tcgggcccct tgttcaggat gtgaccttcc acgggcttga taccggcgga
     1081 gatgagggcc ggaaggtgga tggatccccg cagctcgcgg aggatgaaca gcacctgcag
     1141 ggcccgggcc ggggcgtccg gcgccctcgg catcagcctc caggcgcaga agagcggcat
     1201 acccgccgcc ggcgcggcgt cgatgatcct ctcgccgagt tcgacgatgc ggtcgaggcc
     1261 cggagcgccg gtgaggtggt cgcggcccca gtccgccgcc tgctcccagt agagacgggc
     1321 gccctcggcg gcaccgtgga cggcgcgtcc ctgctcccag aacagcttgg tgacgcccgg
     1381 ttcgaagacg gcgaacacgg cgtcgaccac ttcccagtcc acgtcgccga gcacgccgcc
     1441 ccgaccggcg aagtaaccgg cgaaggggtg ggggtaacca tgggcctcgc tccgggcgaa
     1501 cgtttcagca tgcagcatcc agtcgcggcc gatctgctcg atcgggccgc cggcggcccg
     1561 tacggcctgg gtcaggcggg tgttcacgac aacctccttg gggtagcggc tcagcggtag
     1621 gcgtcgccgt cgggca
//
LOCUS       DASWGG010000419         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2214908, whole genome shotgun sequence.
ACCESSION   DASWGG010000419 DASWGG010000000
VERSION     DASWGG010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214908"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VF954_07740"
     CDS             <1..223
                     /locus_tag="VF954_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531014.1"
                     /translation="AAKQAGVRFARRFVLVVPIGMAVAGLSIGTGRAAYQTAVGQVAV
                     ASGIGAVLACWLWAGRLMRLPDQQRVFFS"
     gene            220..1128
                     /locus_tag="VF954_07745"
     CDS             220..1128
                     /locus_tag="VF954_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9531015.1"
                     /translation="MIRLLVASSLCLWLGATLLLARVRWFARPPLVERLRPYSPGGMA
                     SPGRGGVLSVESFREIVAPVARSVGERVARLFGVSEEVAVRLERVHSPLDVTAFRIRQ
                     LGVATAAFGGAALLMIATRPAVLLALLVVLGAPLLAFLVLEQQLAAASGAWQRRLFLE
                     LPVVSEQLAMLLSAGYSLGAALTRLERRGHGACAQDLARVCGRIRQGLTEVEALREWA
                     SLARVGALDRLVPVLALNREAADLGRLISEEARAIRKDVQRGLVETIERRAQQVWVPV
                     TVATLVPGVIFLAIPFIEALRLFSGS"
     gene            1146..1367
                     /locus_tag="VF954_07750"
     CDS             1146..1367
                     /locus_tag="VF954_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531016.1"
                     /translation="MGDLILRAYVWPRAGLVYLSGLIVKDEYGEGVISAAIAVLIMAF
                     LGVAMWIAFKATLGSATRNVDNQVNQIGH"
     gene            1374..1763
                     /locus_tag="VF954_07755"
     CDS             1374..1763
                     /locus_tag="VF954_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531017.1"
                     /translation="MTRRDDGTGLIATVFGVAVFLLFLLLGVQVVFDLYARTTVTAAA
                     YDAAHTVAGADAGATPAAQAEAEQRARDLLGSYGRRVRFRWSLDPDTVALTVQVHNPS
                     LLPAMVAGPLGIDTIDRTVVLRREAFR"
     gene            1790..2254
                     /locus_tag="VF954_07760"
     CDS             1790..2254
                     /locus_tag="VF954_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531018.1"
                     /translation="MKAVPLKGQLVRPDDTGQVGGVEALAFGVLIFVLGTLVVANAWG
                     VIDAKMAAAGAAREATRAFVEAPTPDAAGALAEAAAADAIAAQGRNPARMHLALSGAF
                     ARCSRVLATVTYRVPLVAVPLLGGFGPGFTVAARHSELVDPYRSGLAGAARC"
     gene            2248..>2590
                     /locus_tag="VF954_07765"
     CDS             2248..>2590
                     /locus_tag="VF954_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531019.1"
                     /translation="MLRPGRPRAGSARLGDRGSVLLLVPAAVLVLVILGAIAVDSAVV
                     FLGQRELANGADAAANDAATAFSDPAFYRAGGTTAIDSRQATAVVEASLAARQMSGVT
                     LDGPPAISISGN"
BASE COUNT          288 a          954 c          933 g          415 t
ORIGIN      
        1 ggcggccaag caggcgggtg tccggttcgc gcgcaggttc gtgctcgtcg tgcccatcgg
       61 gatggccgtg gccgggctgt cgatcggcac cggccgggcc gcctaccaga ccgccgtggg
      121 ccaggtggcc gtcgcctcgg ggatcggcgc ggtgttggcc tgctggctgt gggccggacg
      181 cctcatgcga ctgcccgacc aacagcgggt gttcttctcg tgatccgcct cctcgtcgcg
      241 tcgagcctct gcctgtggct cggagccacg ctcttgctgg cccgggtccg gtggttcgcc
      301 cgcccgccgc tcgtggagcg cctccggccc tacagcccgg gcgggatggc cagtcccgga
      361 cggggcggcg tcctgtccgt cgaatccttc cgggagatcg tggcgccggt cgcccgctcg
      421 gtcggcgaac gcgtcgcccg tctcttcggc gtcagtgaag aggtagccgt gcgcctcgag
      481 cgggtgcact cgccattgga cgtcaccgcc ttccgcatcc ggcagcttgg cgtcgcgacc
      541 gcggcgttcg ggggcgcggc cttgctgatg atcgccaccc gcccggcagt cctcctcgcc
      601 ctcctggtcg tcctcggcgc tccgttgctc gccttcctgg tcctcgagca gcaactggcg
      661 gcggcgtcgg gggcatggca gcggcgcctg ttcctcgagc tcccggtcgt gtccgagcag
      721 ctggccatgc tgctgtccgc cggctacagc ctgggggcgg cgctcacccg cctggaacgg
      781 cgcggccacg gcgcctgcgc ccaggacctg gcgcgggtct gcggtcggat ccgccagggc
      841 ctgaccgagg tggaggcgct gcgcgagtgg gcgtcgctgg ctcgggtcgg cgcccttgat
      901 cgcctcgtcc cggtgctggc cctcaaccgg gaggcggccg acttgggccg gctgatctcg
      961 gaggaggcgc gggcgatccg caaggacgtc cagcgcggcc tggtcgagac catcgagcgc
     1021 cgcgcccagc aggtgtgggt gccggtcacg gtcgccaccc tggtgccggg cgtgatcttc
     1081 ctggccatcc cgttcatcga ggcgcttcgc ctcttctcag gatcgtgatc cgaaaggagc
     1141 cccgcatggg cgacctcatc ctccgggcct acgtctggcc acgcgccggg ctggtgtacc
     1201 tctccgggct gatcgtcaag gacgagtacg gggagggcgt gatctccgcg gccatcgccg
     1261 tgctgatcat ggcgttcctc ggcgtggcca tgtggatcgc cttcaaggcc accctcggca
     1321 gcgccaccag gaacgtcgac aaccaggtca accagatcgg ccactgagcg gccatgactc
     1381 ggcgggacga cggcacgggg ctgatcgcca ccgtcttcgg agtcgcggtg ttcctgctgt
     1441 tcctgctgct gggcgtgcag gtcgtcttcg atctctacgc ccgcacgacg gtcaccgcgg
     1501 cggcatacga cgccgcccac acggttgcgg gcgcggacgc gggcgccact ccagccgctc
     1561 aggccgaggc cgagcagcgg gctcgggacc tgctcgggtc ctacggccga cgggtgaggt
     1621 tccgatggtc cctggacccc gacacggtcg ccctcaccgt gcaggtccac aacccctcgc
     1681 tcctgccggc gatggtggcc gggccgctcg gcatcgacac catcgaccgc accgtcgtgc
     1741 tccggcgaga ggcgttccgt tgaaagctgc tccgttgaaa gcggctcggt tgaaagcggt
     1801 tccgttgaaa gggcagctcg tgcgccccga cgacaccggc caggttggag gggtcgaagc
     1861 cctggccttc ggggtgctca tcttcgtgct cggcacgctg gtcgtggcca acgcgtgggg
     1921 tgtcatcgac gccaagatgg cggccgccgg agcggcccgc gaggcgacgc gagcgttcgt
     1981 cgaggctccc accccggatg cggccggcgc cctcgcagag gcggccgccg ccgacgccat
     2041 cgccgcccag ggccgcaacc cggcccgcat gcacctggcg ttgtccggcg ccttcgcccg
     2101 ctgctcgcgc gtgctcgcca ccgtcaccta ccgcgtcccc ctcgtcgccg tccccctcct
     2161 cggcggcttc gggccaggct tcacggtggc ggcccggcac tcggagctgg tcgacccgta
     2221 ccggagcggg ctggcgggcg cggcccgatg ttgaggcccg ggcggcccag ggccggttcg
     2281 gcgcggctgg gcgaccgggg ctcggtgctg ctgctcgtcc cggccgccgt cttggtcctc
     2341 gtcatcctgg gcgccatcgc ggtcgactcc gcggtggtgt tcctgggcca gcgcgagctg
     2401 gccaacggcg ccgacgccgc ggccaacgac gccgcgaccg ccttctccga cccggccttt
     2461 taccgagccg gcggcaccac ggcgatcgac tccaggcagg cgactgcggt ggtggaggcc
     2521 tcgctggccg cccgccagat gagcggggtg accctcgacg gcccaccggc gatcagcatc
     2581 agcggcaacg
//
LOCUS       DASWGG010000420         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2467354, whole genome shotgun sequence.
ACCESSION   DASWGG010000420 DASWGG010000000
VERSION     DASWGG010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467354"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="VF954_07770"
     CDS             <1..1388
                     /locus_tag="VF954_07770"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HEX9531020.1"
                     /translation="RLEAFGFADAAQTRAALAELTQGLTRRSGLMQQMLPLLLEWLSE
                     APDPDLGLLGLRNLSYGSHRAGRLVETFRESPEAARRLCYLLGTTRLVHERFERDPAL
                     IADLADDAALARRPRSELSQRARRASDWRPDPAYRRSGLRHFARAEQARIQVRDVLGL
                     DELAATEAGLSDLADAVLDAAVAEAAGGAAFAVIGMGRLGGAELSYASDLDVLFVFGD
                     GVAPEAAEAVAAELLATVNGTTPSGRIFPIDANLRPEGKDGALARSLAGYANYYERWA
                     ATWERQALLRARPVAGDAALAARFMEVVAAHVWGRPFTAAEEREIRRLKARVERERIP
                     RGEDPQFHLKLGRGSLSDIEWTVQLLQLRHAVRGAGTMAVLDSLRAAGHIDQGDADVL
                     ADAYRYCERARNRLFLVRGQAGDSLPTQADQLARLARSLDTTPAALRDSYRRVTRRAR
                     AVVERLFYGSG"
     gene            complement(1393..1671)
                     /locus_tag="VF954_07775"
     CDS             complement(1393..1671)
                     /locus_tag="VF954_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531021.1"
                     /translation="MGHVAPEQPGALMPADSDTDSDMDDRPRVARRAAANAMLRAGAR
                     LAAVADALDDLRGQSLDPERRERRDWLLQEQWAARRRYEEAFARFNTP"
     gene            1730..2422
                     /locus_tag="VF954_07780"
     CDS             1730..2422
                     /locus_tag="VF954_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531022.1"
                     /translation="MPAPYHRAIGLDDLYDPDPWGRLVAARALRERAAQRVARRASPL
                     VPRRSPGAANAWLVMVTSKEPAWEDPLALWVERPMTLGQPHEGLFYPDRLGFWAEVRR
                     WSAVLVGRADRSRRWDPGDVLSLVAVVHSSSEPSVLGPVLELTRPRVVLFLDEPGWRA
                     GGLVVDAPVAFSMPDPYRPALSYNGFWGIDPTGRVVGKAPQHPASHKLYDARDMDRFL
                     RAVPLTSSRSLT"
     gene            complement(2411..>3229)
                     /locus_tag="VF954_07785"
     CDS             complement(2411..>3229)
                     /locus_tag="VF954_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531023.1"
                     /translation="EINGLEFLEDTSLATDGSASIANGQHLVDLSSVFPGVTATLHLI
                     SPAVVVGPGAAVAPPGTPTTVGKTAQLEADFMVSAPTSVPAPVLGTTPLPVQVTFPMQ
                     LHGASATGGLTYIDCNPPATPPTPITATVHVTTDEVWLSGGPAKVSVDGGPSVPDGVP
                     TSVGPVDFAGAVQDAKEAEGATGRVAAPAPTIPDLVSGVFNGAAITVTAMLPDGLPVS
                     VPTSTVDDAITTATHDLNAYLGAAGSALGITVAGADVTVHDITCGELGNVSSGK"
BASE COUNT          433 a         1168 c         1178 g          450 t
ORIGIN      
        1 cgcgcctcga ggccttcggc ttcgccgacg cggcccagac ccgcgccgcc ctggccgagc
       61 tgacccaggg cctgacccgg cggtccgggc tcatgcagca gatgctcccg ctcctgctcg
      121 agtggctgtc cgaggccccc gatcccgacc tcggcctgct cggcctgcgg aacctgtctt
      181 acggcagcca ccgggccggg cgcctggtgg agaccttccg cgagtcgccg gaagcggccc
      241 ggcggctgtg ctacctgctc ggcacgaccc ggctggtgca cgagcgcttc gagcgcgacc
      301 ccgccctcat tgccgacctg gccgacgacg ccgccctggc ccgccggccg cgctccgagc
      361 tgtcgcagcg ggcccggcgg gcctccgact ggagaccgga cccggcctac cgccggagcg
      421 ggctgcgcca cttcgcccgg gccgagcagg cccgcatcca ggtccgcgac gtcctcggtc
      481 tcgacgagct ggccgcgacg gaggccggac tcagcgacct ggccgacgcc gtgctcgacg
      541 cggcggtggc ggaggcggcg ggcggcgccg cgttcgcggt gatcgggatg ggccgcctag
      601 gcggggcaga gctgtcctac gcgagcgacc tcgacgtcct gttcgtcttc ggcgacggtg
      661 tggcacccga ggcggccgag gcggtcgccg cggagctgtt ggccacggtc aacggcacga
      721 ctccgagcgg ccgcatcttt cccatcgacg ccaacctccg acccgagggc aaggacgggg
      781 cgctggcccg cagcctcgcc ggctacgcca actactacga gcgctgggcg gcgacctggg
      841 agcgccaggc cctgctgcgg gcccgcccgg tcgcgggcga cgccgccctg gcggcgcggt
      901 tcatggaggt ggtcgccgcc cacgtgtggg gccggccctt caccgcggcc gaggagcggg
      961 agatccggcg gctcaaggca cgggtcgagc gggagcggat cccgcggggc gaggacccgc
     1021 agttccacct gaagctgggc cggggctcgc tctccgacat cgagtggacg gtccagctgc
     1081 tgcagctgcg ccacgcggtg cggggggcag ggaccatggc cgtgctcgat tcgctgcggg
     1141 cggccgggca catcgaccag ggcgacgccg acgtgctggc cgatgcgtac cgctactgcg
     1201 agcgggcccg caacaggctg ttcctcgtgc gcggccaggc aggcgactcg ctgcccaccc
     1261 aggccgacca gctggcccgg ctggcccgaa gcctcgacac cacgccggcc gccctgcggg
     1321 acagctaccg ccgggtcacc cgccgggccc gggcggtcgt ggaacgattg ttctacggct
     1381 cggggtagcg cctcacggcg tgttgaagcg ggcgaaggcc tcctcgtacc gccggcgggc
     1441 cgcccactgc tcctgcaaga gccagtccct ccgctcccgc cgctccggat cgaggctctg
     1501 gccccgcagg tcgtccaggg cgtcggccac cgccgccagg cgggccccgg cccgcaacat
     1561 ggcattggcc gccgcccgcc tggcgaccct cggtcggtcg tccatgtccg aatcggtgtc
     1621 cgagtcggct ggcatgagcg cccccggttg ctccggggcg acgtgaccca cgtcacaagt
     1681 gtacaaaacg gcgagaagga gcggggacac ccgccgtcgc tcgacaaagt tgcccgctcc
     1741 gtatcatcgg gccatcggcc tcgacgacct gtacgacccg gacccatggg ggcgcctggt
     1801 cgcggcgcgg gcgctccggg aacgggcggc acaacgtgtc gcccgccggg cctcgccact
     1861 ggtgccgcgc cggtcgccgg gggcggcgaa cgcgtggctg gtgatggtca cgtccaagga
     1921 gccggcttgg gaggacccgt tggcgctgtg ggtggagcgg ccgatgacgc tgggccagcc
     1981 ccacgagggg ctcttctatc ccgatcgcct cgggttctgg gccgaggtca ggcgctggtc
     2041 agccgtgctc gtcggccggg cggaccggtc ccgccgctgg gacccgggcg acgtcctcag
     2101 cctggtggcg gtcgtgcaca gctcctcgga gccctcggtc ctgggccccg tgctcgagct
     2161 gacccggccg cgggtggtgc tcttcctgga cgagccgggc tggcgggccg gcgggctggt
     2221 ggtcgacgcg ccggtggcct tctcgatgcc ggacccgtac cggccggcgc tgagctacaa
     2281 cgggttctgg ggcatcgacc ccaccggccg ggtggtaggc aaggccccac agcacccggc
     2341 cagccacaag ctgtacgacg ccagggacat ggaccggttc ctgcgggccg tgcccctcac
     2401 atcctcgcgg tcacttacct gagctgacgt tgccaagctc accgcacgtg atgtcgtgca
     2461 cggtgacgtc ggcgccggcc actgtgatcc cgagcgcgga gccagcagcc cccaagtagg
     2521 cgttcagatc atgggtcgcg gtggtgatcg cgtcatcgac cgttgaggtc ggaacggaca
     2581 cgggtaggcc gtcggggagc atcgcagtga ccgtgatggc agccccgttg aagacgccgc
     2641 tcaccaggtc aggaatggtc ggagccggcg cggccactct ccccgtcgcg ccctccgcct
     2701 ctttcgcatc ttggaccgcg ccggcgaaat caacggggcc aaccgaggtg ggcacgccat
     2761 cgggcacaga cggcccgcca tcgacggata ccttcgccgg accgccggaa agccacacct
     2821 catccgtcgt cacatggacg gtcgcggtta tcggggtcgg aggggtagct ggcggattgc
     2881 agtcgatgta ggtgaggccg ccggtggcgc tcgccccatg cagttgcatg ggaaacgtga
     2941 cctggacagg aagcggggtc gtgccgagga cgggtgcagg gacgctcgta ggcgcgctta
     3001 ccatgaagtc cgcttccagt tgcgccgtct tgcccaccgt cgtcggagtg cccggggggg
     3061 ccacggccgc acccggccca acgaccaccg ccggcgagat gagatgcaag gtcgcggtca
     3121 cgccggggaa gacggagctc aggtccacca ggtgttggcc gttggcgatc gaagcggagc
     3181 cgtccgtcgc cagactcgta tcctctagaa attccagtcc attgatctc
//
LOCUS       DASWGG010000421         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_283832, whole genome shotgun sequence.
ACCESSION   DASWGG010000421 DASWGG010000000
VERSION     DASWGG010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283832"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VF954_07790"
     CDS             <1..703
                     /locus_tag="VF954_07790"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX9531024.1"
                     /translation="ARPLGAIFDPGVVGHALQGRVGQVWATAGLLLVLAVPVLWALVR
                     QGQAAVGATGWRVSAAAVTIGLLRAPALISHSSEGRQPWLGSAADLVHLGGVAVWLGG
                     LAVMLAVVLPRRRPEEMERLVPMFSKVAFGAVAAIVVAGTFMSWQLVGSVHALVTSRF
                     GRLLLLKIGLFGATLAAARLSKGWVDRRLDLAVLTGADRETVRPFVYSVVAEAGLAIG
                     VLSVAGALVATSPAH"
     gene            817..1368
                     /locus_tag="VF954_07795"
     CDS             817..1368
                     /locus_tag="VF954_07795"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9531025.1"
                     /translation="MTLTQTHPEPAVAAPAPGRYVLDTDRTIVEVAVRHFAVAQVRGR
                     FRALGGRLTVAKRAEDSWLRVDLDAASLATGHRERDASLQGAAMLASSEFPVIRLETA
                     GVVARDGSTYDVVADLYIRDAVVETTARVWLVDFGDDHIRLAARLAVSRSSLGLGWSS
                     EVERYGVLVADTVRVTVGAEFRR"
     gene            1365..2306
                     /locus_tag="VF954_07800"
     CDS             1365..2306
                     /locus_tag="VF954_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEX9531026.1"
                     /translation="MSPALPRRRWAWAVLASAGALAAWALVGIAASRPSSAASQSVMI
                     QNYAFSPSALTVHAGDTVTWTNMDDAPHTVTTSSGPQAIDSPQLNKGDSFSFTFTKVG
                     TYQYYCSVHPDMKASVTVVDQGSSGGQPGAGSGGQSGGQGGNGSGSPGSSGGPGSGGG
                     QPSGGGSSGGGGSSGGGQPSGGGGSPGGPPPSSCTGQNVADAMAAPFVAHLQHAHLEA
                     SPGQQAGDLTNTDQYVKTHTVLIEQMTAPAFGMLLASFTGVDPFVTHVDHAHLQESPA
                     QQAGDLLNVSQYVNTHTVLIEDMTAPTTGSATGTYGC"
BASE COUNT          305 a          849 c          853 g          376 t
ORIGIN      
        1 cgcccgaccg ctgggcgcca tcttcgatcc gggcgtcgtc ggtcacgcac tgcaaggccg
       61 ggtgggccag gtgtgggcca cggccggtct cctgctggtc ctggccgtcc cggtgctctg
      121 ggcgctcgtg cggcagggcc aggcggccgt cggcgccacc ggttggcggg tctcggctgc
      181 cgccgtcacc atcggcctgc tgcgggcgcc ggcgttgatc agccactcga gcgagggccg
      241 gcagccgtgg ctgggatcgg cagccgacct ggtccatctc gggggcgtcg ccgtctggct
      301 cggtgggctt gccgtgatgc tcgccgtcgt gttgccgcgc cggcggcccg aggagatgga
      361 acggctcgtg cccatgttct cgaaggtggc cttcggcgcc gtcgcggcca tcgtcgtggc
      421 cgggacgttc atgtcctggc agctggtcgg cagcgtgcac gctctggtga ccagccgctt
      481 cggtcgcctc ctcctgctga agatcgggct gttcggggcc acgctggccg ccgcccggct
      541 gagcaagggc tgggtcgatc ggcgcctcga cctcgccgtg ctcacgggcg ccgaccgcga
      601 gaccgtgcgg ccgttcgtct actccgtcgt ggccgaggcc ggtctcgcca tcggtgtgct
      661 gagcgtggcg ggcgcattgg tcgcgaccag tcccgcccat tgaccccgca gttcgttcga
      721 cgcgcccgag aggttcgaga tgttcgagag gttcgagagg ttcgagaggt tcgagaggtt
      781 cgagatgaag acacccccca acccccggaa gggcccatga cgcttaccca aacccacccc
      841 gagcccgccg tggccgcgcc ggcgcccggc cggtacgtgc tcgacaccga tcgcaccatc
      901 gtcgaggtgg cggtgcggca tttcgctgtc gcccaggtgc gaggccggtt ccgtgcgctc
      961 ggaggtcggc tcacggtggc caagcgggcg gaggactcct ggctacgcgt cgacctcgac
     1021 gcggcctcct tggccaccgg ccaccgcgaa cgcgacgctt cgctccaggg agccgccatg
     1081 ctcgcctcca gcgagtttcc ggtcatccgg ttggagaccg ccggggtcgt ggcccgggac
     1141 ggctcgacct acgacgtggt cgccgacctc tacatccggg acgcggtggt cgagacgacc
     1201 gcgagggtgt ggctcgtcga cttcggcgac gaccacattc gtctggccgc ccggctggcg
     1261 gtgtcgcgct cgtcgctcgg tctgggatgg agcagtgagg tcgagcggta cggcgtgctc
     1321 gtcgccgaca ccgttcgcgt caccgtcgga gcggagttcc ggcgatgagc ccggctcttc
     1381 cccggcggcg gtgggcatgg gcggtcctgg cgagcgctgg cgccctggcg gcgtgggcgc
     1441 tggtgggaat cgcggcatcg cgcccctcgt cggccgcctc gcaatccgtc atgatccaga
     1501 actacgcctt ctcaccctcc gccctcaccg tccacgccgg cgacacggtc acgtggacca
     1561 acatggacga cgccccgcac acggtgacga cgagcagcgg gccgcaagcg atcgactcgc
     1621 cgcagctcaa caagggcgac agcttcagct tcaccttcac caaggtcggc acgtaccagt
     1681 actactgctc cgtccatccc gacatgaagg ccagcgtcac ggtggtggac caggggtcgt
     1741 ccggcgggca gcctggcgcc gggtcgggcg gccagtccgg tgggcagggg ggaaatggga
     1801 gcggttcgcc ggggtcgtcc ggtggccccg gctcgggcgg aggtcagccg tcgggcggcg
     1861 gctcgtcggg tggcggcggc tcgtcgggtg gcggtcagcc atcgggtggt ggcggctcgc
     1921 cgggtggccc accaccgtcg agctgcaccg gccagaacgt cgccgatgcc atggccgcgc
     1981 cgttcgtcgc ccatctccag cacgcccatc tggaggcatc gcccggccag caggccggcg
     2041 atctcaccaa caccgaccag tacgtcaaga cgcacaccgt gctgatcgag cagatgaccg
     2101 ccccggcctt tgggatgctg ctcgcgtcct tcacgggggt cgatccgttc gtgacccacg
     2161 tcgaccacgc ccacctgcag gagtctccgg cccagcaggc aggcgacctg ctcaacgtca
     2221 gccagtacgt caacacccac accgtgctga tcgaagacat gacggcgccg accaccggat
     2281 cggccacggg tacctacggg tgctgacggc tcgccaccgc cgctcccgtc cctcaggagc
     2341 ggcggtcggg cgactggtcc tcgccgccgg gttggcggcg ctc
//
LOCUS       DASWGG010000422         5376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_285249, whole genome shotgun sequence.
ACCESSION   DASWGG010000422 DASWGG010000000
VERSION     DASWGG010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285249"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /gene="trpD"
                     /locus_tag="VF954_07805"
     CDS             complement(<1..633)
                     /gene="trpD"
                     /locus_tag="VF954_07805"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX9531027.1"
                     /translation="MDLGLASVGGWPAVLGKLLASCDLTGEEASCALTEIFQGKATSA
                     QIAAFAVGLRIKGETVEEMSALVRTMLDFAEPVPLPAGFDLVDTCGTGGDRSHTINVS
                     TLAAFVLAGAGVKVCKHGNRAASSACGSADVLEALGVVIDLGPAGVARCVREAGMGFC
                     FAPRYHAASRHAVPTRRELGVATVFNFLGPLANPARPRRQVVGVSDPAMAA"
     gene            complement(659..850)
                     /locus_tag="VF954_07810"
     CDS             complement(659..850)
                     /locus_tag="VF954_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX9531028.1"
                     /translation="MDPAVFYPTSDEEAEAAKEVCARCPVREACLEFALASRERDGVW
                     GGATERERRRMIRHRRKSA"
     gene            1082..2749
                     /locus_tag="VF954_07815"
     CDS             1082..2749
                     /locus_tag="VF954_07815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX9531029.1"
                     /translation="MSATPFRRDAALARLRDEDFDVLVVGGGVTGCGIALDAAARGLR
                     TALVERDDFASGTSSKSSKLIHGGLRYLQQKEYRLVYENLSERQRLLDNAPHLVSPLP
                     FLIPLFGKNGIAGQGLAKAYSTALWLYDLTGGIRIGKRHRRIGKEEVLAHMPGLRADR
                     LVAGFLYWDAQADDARLTLTLARTAADHGATVANYAGVTALTKDDGRLVGATVHPRDG
                     DPFPVVAKVIVNAAGVWADDVRALAEGHHPHSLRPAKGIHLTLPRAKLPVDVAAVIPV
                     PGDRRSIFVVPWGDEVYLGTTDTDYEGPLEDPPVTPEDVSYVLNGINAWVAGTLVPED
                     VVGAWAGLRPLVRAATTERTADLSRRHVVSVDDEGLVSITGGKLTTYRKMAADTVDEV
                     ARRLSLSALRRSPTRKLRLHGARGSAGLRGPEAAARLGVPEPVLAHLVSRFGAEARTV
                     AAMVVNRPALGAPLVPGLPYLRAEAVFSARYEMVHTLADVLSRRTRALLLSRDAAAGA
                     AADTAEVIAADLGWSPAETALQVAAFRTLAERQRVAAGLPASVAVAP"
     gene            2746..4326
                     /locus_tag="VF954_07820"
     CDS             2746..4326
                     /locus_tag="VF954_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9531030.1"
                     /translation="MSAPGAGAKVVPTPPIELQPSPAGVTARLPARPVPVPDEVVERL
                     RSVCSSVSVDPAERAEAGRDWWPVALTWALAGSAPALPAVVARPASAVEVAAVLAVCN
                     QARVPVTAAAGRSGVCGGAVPVFGGVSLDLCGLSGVVALDSPSLVVDVLAGTFGVPLE
                     ESLRAEGLTLGHWPQSIELSTVGGWLACRSAGQYSTRYGKIEDMVVGLEVALADGRLV
                     HLGGAAPRAAVGPDLVQMFVGSEGTLGVITSARLRVRPAPEVERRAAYGFASFEEGLD
                     ACRRVLRRGATPAVLRLYDAVESARSFGTSAANVMIVLDEGEGVIVEASMAVVHEECE
                     GAEVLDDALVGRWLAHRNDVAALASLYRSGIVVDTCEVAAAWSALPGLYAAGIAALRS
                     VPGTMVASAHQSHAYPDGACLYFTFAGRPADGAADSAAESAAGADPGDAFAWSDRYYV
                     AAWSAVTDAVLAAGGALSHHHGVGLNRARFMARALGPGLDLLAAVKAALDPNGILNPG
                     KLGLPSPFGPVGWPPPDA"
     gene            4337..>5376
                     /locus_tag="VF954_07825"
     CDS             4337..>5376
                     /locus_tag="VF954_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX9531031.1"
                     /translation="MSILVLDVGTSSVRAAIVRPDATIGPVHQWPVLPSTPMQGFVEL
                     DAAEMAAAALGVASAALAEGGPVLAVGIANQRASTVVWDRATGRPVGPGIGWQDLRTV
                     MLCLTLQAQGVRLAPNTSATKAAVLLDMADPGRTGDLCVGTVDSWVAWNLSGGRLHVT
                     DQTNAGMTELLCPDGSDWDDGVLDVLRIPRSALPRIVDSSGVVGPASALDGAPPIAGL
                     AGDQQASLVGQGCTRPGLAKITFGTGGMLDVCVGAERPAFPKRGAGGTFPIAAWRKNG
                     RITWGVEAIMLSAGTCVEWLRDDLGVIASVAESETVAAGCSDTGDVWFVPALLGLGTP
                     VWDYGARGTLVGL"
BASE COUNT          653 a         2015 c         1952 g          756 t
ORIGIN      
        1 ggccgccatg gcggggtcgc tgacgcccac cacctggcgt cggggacggg ccgggttggc
       61 cagcggtccg agaaagttga aaacggtggc cacgccgagc tcccgccgcg tcggcaccgc
      121 gtggcgggac gcggcgtggt agcggggcgc gaagcagaac cccatcccgg cctcccggac
      181 gcatcgggcc acgccggccg ggccgagatc gatcaccaca ccgagcgcct ccaggacgtc
      241 cgccgacccg caggccgacg acgccgcccg gttgccgtgc ttgcacacct tgacgcccgc
      301 ccccgccagc acgaaggcgg ccagggtcga gacgttgatg gtgtggctcc ggtcgccacc
      361 cgtgccacac gtgtccacca ggtcgaagcc ggccgggagc gggaccggct ccgcgaagtc
      421 cagcatggtc cgcaccaggg ccgacatctc ctcgaccgtc tcgcctttga tgcgcagccc
      481 gaccgcgaag gccgcgatct gcgccgacgt ggccttgccc tggaagatct cggtcagggc
      541 gcacgaggcc tcctccccgg tcaggtcgca gctcgccagg agcttgccga gcacggccgg
      601 ccacccgccg acggaggcca ggccgagatc catgcctgga ggctacctgg ggaccggttc
      661 aggcggactt gcggcgatgg cggatcatgc gacggcgctc acgctcggtg gcgccgcccc
      721 acacaccatc gcgctcccgg ctggccagcg cgaactcgag gcaggcctcc cgtaccgggc
      781 atcgggcgca gacctccttg gccgcttccg cctcctcgtc ggaggtgggg tagaagaccg
      841 ccggatccac tccccggcag gctgcacgct gccgccagga caggttcaac aggtatcact
      901 tccttgcgct cgaacaatct caccgccggt tcgcgtccgc tcctaccctc cgcgtcaacg
      961 agctacacgg cggacgggct cagcgactgc cggggaccag tatccctgat catcccggtg
     1021 agttccagta ctttccatct ttgcggaggc gaagacgccg gggccgcggc aagctgacgt
     1081 gatgagcgcc accccgttcc ggcgcgacgc cgccctggct cggctgcgcg acgaggactt
     1141 cgacgtgctg gtcgtcggtg gaggcgtcac cggctgcggc atcgccctcg acgccgccgc
     1201 ccgggggctg cgcaccgccc tggtcgaacg cgacgacttc gcctcgggca cctcctccaa
     1261 gtcctccaag ctcatccatg gcggactgcg gtacctccag cagaaggagt accgcctcgt
     1321 gtacgagaac ctgtccgaac gccagcggct gctcgacaac gcgccccacc tggtgtcgcc
     1381 gctcccgttc ttgatccccc tcttcggcaa gaacgggatc gccggccagg gcctggccaa
     1441 ggcgtactca accgcgctct ggctctacga cctgacgggc gggatccgga tcggcaagcg
     1501 ccatcgccgc atcggcaagg aggaggtgct cgcccacatg cccggcctgc gggccgaccg
     1561 cctggtcgcc ggcttcctgt actgggacgc ccaggccgac gacgcccggc tgaccctgac
     1621 cctggcgcgg acggccgccg accacggcgc caccgtcgcc aactacgccg gtgtcaccgc
     1681 cttgaccaag gacgacggcc ggctggtcgg cgcgacggtc cacccgcgcg acggtgaccc
     1741 tttccccgtc gtggccaagg tgatcgtgaa cgcggccggc gtgtgggccg acgacgtccg
     1801 ggccctcgcc gagggccacc atccccacag cctccgcccg gccaagggca tccatctgac
     1861 gctgccgcgg gccaagctcc ccgtcgacgt cgccgccgtc atccccgtcc ccggggaccg
     1921 gcgctccatc ttcgtcgtgc cgtggggcga cgaggtctat ctcgggacga cggacaccga
     1981 ctacgagggc ccgctagagg atcccccggt cactcccgag gacgtgagct acgtgctgaa
     2041 cggcatcaac gcctgggtgg ccggcacgtt ggtgcccgag gacgtggtgg gcgcgtgggc
     2101 cggcctgcgc ccgctcgtca gggcggccac caccgagcgc accgcggacc tcagccgccg
     2161 acatgtggtg tcggtcgacg acgaggggct ggtgtcgatc acgggaggca agctgaccac
     2221 gtaccgcaag atggccgccg acaccgtcga cgaggtggca cgccggctgt cgctcagcgc
     2281 cctgcgccgg tcgcccaccc ggaagctccg cctgcacggc gctcggggca gcgccgggct
     2341 gcgcggcccg gaagcggccg cccgcctggg ggtcccggag ccggtgctgg cccacctggt
     2401 cagccgcttc ggggcggagg cccggacggt ggccgccatg gtcgtcaacc gcccggcgct
     2461 gggcgcgccg ctggtgcccg ggttgcccta ccttcgggcc gaagccgtct tctcggcgcg
     2521 ctacgagatg gtccacaccc tcgccgacgt gttgagccgc cggaccaggg ccctcctgct
     2581 gagccgcgac gccgccgccg gcgccgccgc cgacaccgcc gaggtgatcg cagccgacct
     2641 cggttggtcc ccggcggaga cggccctgca ggtagccgcg ttccggaccc tggccgaacg
     2701 gcagcgggtc gcggcgggcc tgccggcttc ggtcgcggtc gcaccgtgag cgcgccgggc
     2761 gcgggcgcga aggtggtccc cacgccaccg atcgagctcc agccgtcccc ggccggggtg
     2821 accgcccgcc tgccggcgcg ccccgtgccc gtccccgacg aggtggtcga gcggctccgg
     2881 tccgtgtgct cctcggtgtc ggtggacccg gccgagcggg ccgaggccgg gcgggactgg
     2941 tggccggtcg ccctgacgtg ggccctggcc ggctcggccc ccgccctgcc ggcggtggtc
     3001 gcccggccgg cctccgccgt cgaggtggcg gccgtgctcg cggtgtgcaa ccaggcccgg
     3061 gtgccggtga ccgccgcggc cgggcgctcc ggcgtgtgcg gcggcgccgt acccgtcttc
     3121 ggcggcgtca gcctcgacct gtgcgggctg tccggcgtcg tcgccctgga ctcgccgtca
     3181 ttggtcgtcg acgtgctggc ggggaccttc ggcgtgccgc tggaggagtc gctgagggcc
     3241 gagggcctga cgctcggcca ctggccccag tccatcgagc tgtccacggt cggcggctgg
     3301 ctggcctgcc gctcggccgg ccagtactcc acccggtacg ggaagatcga ggacatggtc
     3361 gtcgggctgg aggtcgccct ggccgatggc cgcctggtgc acctcggggg cgccgccccc
     3421 cgggccgccg tcgggccgga cctggtgcag atgttcgtgg gcagcgaggg caccctcggc
     3481 gtgatcacct cggcccggct ccgggtccgt cccgcgccgg aggtggaacg gcgggcggcg
     3541 tacgggttcg cgtccttcga agaggggctg gacgcctgcc gtcgggttct gcggcgggga
     3601 gccacgccgg cggtgctccg gttgtacgac gcggtcgagt cggctcgttc cttcgggacc
     3661 tcggccgcca acgtgatgat cgtcctcgac gagggcgagg gcgtgatcgt cgaggcctcg
     3721 atggcagtgg tccacgagga gtgcgagggg gctgaggtcc tggacgacgc gctggtcggg
     3781 cgttggctgg cccatcgcaa cgacgtggcc gcgctggcgt cgctctaccg gagcggcatc
     3841 gtggtggaca cctgcgaggt cgccgcggcc tggtcggccc tgcccgggct gtacgccgcg
     3901 ggcatcgccg cgctgcgctc ggtgcccggg accatggtgg cctcggcgca ccagtcccac
     3961 gcctaccccg acggggcgtg cctctacttc accttcgccg gccggcccgc cgacggtgcc
     4021 gccgacagtg ccgccgagag tgccgccggc gctgaccccg gcgacgcctt tgcgtggagc
     4081 gaccggtact acgtggcggc ctggagcgcg gtgaccgacg ccgtgctggc cgcgggcggc
     4141 gccctgagcc atcaccatgg ggtcggcctc aaccgggccc ggttcatggc ccgagccctc
     4201 ggcccgggcc tggacctgct cgccgccgtc aaggccgccc tcgaccccaa cggcatcctc
     4261 aaccccggaa agctcgggct gccgtcgccg ttcgggccgg ttggttggcc gcccccggac
     4321 gcctagtttc tgggccgtga gcattctcgt cctcgacgta ggcaccagca gcgtccgcgc
     4381 cgccatcgtc cggcccgacg ccaccatcgg gcctgtgcac cagtggcccg tgctgccgtc
     4441 gacgccgatg cagggtttcg tggagctcga cgcggccgag atggcggcgg ccgccctggg
     4501 cgtggcgtcg gccgctctgg ccgaaggcgg cccggtgctc gccgtcggca tcgccaacca
     4561 gcgcgcgtcc acggtggtat gggaccgggc gacgggccgc ccggtgggac cgggcatcgg
     4621 ctggcaggac ctgcgcacgg tgatgctgtg cctgacgctg caggcccagg gtgtgcgtct
     4681 cgcgcccaac acctcggcca ccaaggccgc cgtgctgctc gacatggccg acccgggccg
     4741 caccggcgac ctctgcgtgg gcaccgtcga cagctgggtc gcctggaacc tctccggcgg
     4801 ccggctccac gtcaccgacc agaccaacgc gggcatgacc gagctgctgt gcccggacgg
     4861 gtcggactgg gacgacggcg ttctggacgt cctgcgcatc ccgcgctccg ccctgccccg
     4921 catcgtcgac tcgtcgggcg tcgtcggccc ggcgtcggct ctcgacggtg ccccgcccat
     4981 cgccggcctc gcaggtgacc agcaggcctc gctcgtcggc cagggctgca cccggcccgg
     5041 tctggccaag atcaccttcg gcaccggcgg gatgctcgac gtgtgcgtcg gcgcggagcg
     5101 gccggcgttc ccgaagcggg gagccggggg caccttcccc atcgcggcct ggaggaagaa
     5161 cggccgcatc acctggggtg tcgaggcgat catgctgtcg gccgggacgt gtgtggagtg
     5221 gctgcgcgac gacctcgggg tgatcgcgtc ggtggccgag tccgagacag tggcggctgg
     5281 atgctccgac actggtgacg tgtggttcgt gcccgctctg ctcgggctgg gcacgcccgt
     5341 gtgggactac ggcgcccgtg gcacgctggt cgggct
//
LOCUS       DASWGG010000423         2787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_288390, whole genome shotgun sequence.
ACCESSION   DASWGG010000423 DASWGG010000000
VERSION     DASWGG010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288390"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VF954_07830"
     CDS             complement(<1..397)
                     /locus_tag="VF954_07830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX9531032.1"
                     /translation="MEALEKELAGALLGSEVSGSDVWVRVDRAAWRRAVACCRDGLGF
                     NYFTFLSGIDWKADTDLAGEKLWDPNPPGRGDQAPGEGWGTGLAGGDSRFQVMARLQR
                     VPGSVGVTLKADLDEAEPRVASIVDLFRGA"
     gene            complement(429..932)
                     /locus_tag="VF954_07835"
     CDS             complement(429..932)
                     /locus_tag="VF954_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX9531033.1"
                     /translation="MGLVEQGRIPKPLTTLLNRARKYSLWVYQWGLACCAIEMGAAFG
                     SPRYDVMRLGVIPFPASARQADLVVISGTVTDKMVPPIRRLYEQMPDPKYVISMGSCA
                     NCGGPYWDSYSVTKGVDQIIPVDVYVPGCPPRPEALLEGIVLLQQRIQNEDMSRRWRG
                     EPIVVGG"
     gene            complement(923..1288)
                     /locus_tag="VF954_07840"
     CDS             complement(923..1288)
                     /locus_tag="VF954_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX9531034.1"
                     /translation="MSDFLRSYLTVVFFGAAAALLVGATLGLGALVRPHRPQPQKYIA
                     YESGVDPVGTGWSQSQIRYYVFALLFVMFDVEAVFIFPWAVRLEAFGYFGLVEMVVFI
                     VILALGLVYAWRKGVLRWV"
     gene            1353..>2787
                     /locus_tag="VF954_07845"
     CDS             1353..>2787
                     /locus_tag="VF954_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531035.1"
                     /translation="MTPDTRAAGPADARAADPAEDPAGGIVAGLVADLVAVEVPVGGR
                     VMVVSDLRLGRDATPASAAAVAGLAQAIDAWDGPGVLVFAGGLFDLLTGGPLHPGAAA
                     AALAAHPKLAGAVTGFCGAERRRILVLPGSRDGRLAWDESTRDAVASGLGAEVALAVE
                     LEVATGAGLRRVRVEPGHRFDARFAPGDPRSPSDSPLGHHVLTELMPTLPGYASWLSG
                     PEDLRAQADLPRFLVSRLTYRRLARHGWWLLIPFVIAVALKVPLVYAIAARGPIHGAG
                     MRSWGGRIGLLGVTTVVDLLLVALAAFYVSRRAWREMSPVAVGEGGLDSNNCARDTAR
                     DLVTAGLAGLVTGHTLQPELAHLGTGFYANAGGGTVVLDEVPGRAGLPPVFLPFRLLS
                     WIELEAGAELHVRLLHSRVEVPGATLLERLAARRPALREPRPVVVGAFPAGRPWPPVT
                     DPVRRQRRVRRWAAIAIAVAGVLDLLSG"
BASE COUNT          372 a         1017 c         1021 g          377 t
ORIGIN      
        1 ccgctccgcg gaagaggtcg acgatcgagg ccacccgcgg ctcggcctcg tccaggtcgg
       61 ccttgagggt gacgccgacc gagcccggca cccgctggag ccgcgccatc acctggaagc
      121 gggagtcgcc gccggccagg ccggtccccc agccctcgcc gggggcctgg tcgccgcggc
      181 cgggcgggtt ggggtcccag agcttctcac cggcgaggtc ggtgtcggcc ttccagtcga
      241 tgccggacag gaaggtgaag tagttgaaac cgaggccgtc gcggcagcac gccacggccc
      301 ggcgccacgc ggcgcggtcc acccggaccc acacgtcgct gccggagacc tccgagccga
      361 ggagggcacc ggccagctct ttctccagag cctccactat cccgacgccg atttgatcgg
      421 tggatgcgtc agccgccgac gacaatgggc tcacctcgcc agcgccggct catgtcctcg
      481 ttctggatgc gctgctgcaa caggacgatg ccttcgagca gggcctcggg gcggggcggg
      541 cagcccggga cgtacacgtc gacggggatg atctggtcca cgcccttggt gacggagtag
      601 gagtcccagt acgggccccc gcagttggcg cagcttccca tggagatcac gtacttcggg
      661 tccggcatct gctcgtacag ccgccggatc ggcggcacca tcttgtccgt gacggtgccg
      721 gagatgacca ccaggtcggc ctgccgggcg ctcgccggga acgggatgac gccaaggcgc
      781 atgacgtcgt accggggcga gccgaacgcc gcccccatct cgatggcgca gcacgccagc
      841 ccccactggt agacccacag cgagtacttg cgggccctgt tgagcagggt ggtcagaggc
      901 ttggggatcc gcccctgctc gactagaccc accgcaagac gcctttgcgc caggcgtaga
      961 ccaggccgag ggccaggatg acgatgaaga cgaccatctc caccaggccg aagtacccga
     1021 acgcctccaa gcgcacggcc cacgggaaga tgaacacggc ctcgacgtcg aacatgacga
     1081 agagcagggc gaacacgtag tagcggatct ggctctgcga ccacccggtg cccaccgggt
     1141 cgacgccgct ctcgtaggcg atgtacttct ggggctgcgg gcggtgcggc cgcaccaggg
     1201 caccaagccc tagggtggcg cctaccagca gcgcggccgc cgcaccgaag aagaccacgg
     1261 tgaggtagct gcggaggaag tccgacatcg cccgggcagc gtactcaggg ggttcatacc
     1321 ccgccaaagc aggctgacaa gatggacgtc acatgacacc cgacacccgg gcggccggtc
     1381 ccgccgacgc ccgcgccgcc gatccggccg aggatccggc cggcggtatc gtcgccggtc
     1441 tggtcgccga tctcgtcgcc gtggaggtgc ccgtgggcgg ccgggtcatg gtcgtgtccg
     1501 acctccgcct gggccgggac gccacacccg cgtcggccgc cgcggtggcc gggctggccc
     1561 aggccatcga cgcctgggac gggccggggg tgctggtgtt cgcgggcggc ctgttcgacc
     1621 tgctgaccgg cgggccgctc catccgggcg cggcggcggc cgccctggcc gcccacccga
     1681 agctggccgg cgccgtgacg gggttctgcg gggccgagag gcggcggatc ctcgtgctgc
     1741 ccgggtcacg ggacggccgg ctggcgtggg acgagagcac gcgggacgcg gtggcgtcgg
     1801 ggctcggggc ggaggtggcc ctggcggtgg agctggaggt cgccaccggt gccgggctgc
     1861 ggcgagtgcg ggtcgagccg ggccaccgct tcgacgcccg gttcgcgccc ggtgacccga
     1921 ggagcccgag cgactccccg ctcgggcacc acgtgctgac cgagctcatg ccgacgctcc
     1981 ccggctacgc cagctggctg tccggacccg aggacctccg ggcccaggcc gacctgcccc
     2041 ggttcctggt gtcgcggctc acgtaccgcc ggctggccag gcacggctgg tggctgctga
     2101 tccccttcgt catcgccgtc gccctcaagg tccccctcgt ctacgccatc gcggcccggg
     2161 gccccatcca cggggcgggc atgcgctcgt ggggagggcg gatcggcctg ctcggcgtca
     2221 ccaccgtcgt cgacctgctg ctcgtggcgc tggcggcgtt ctacgtcagc cgccgggcat
     2281 ggcgggagat gagccccgtg gccgtcggcg agggcggcct cgactccaac aactgcgccc
     2341 gggacacggc ccgggacctg gtcaccgcgg ggttggcggg attggtcacg ggccacaccc
     2401 ttcagcccga gctcgcccat ctcggcacag ggttctacgc caacgcgggc gggggcacag
     2461 tggtgctcga cgaggtcccc ggccgggccg ggctgccgcc ggtgttcctg cccttccggt
     2521 tgctgtcgtg gatcgagctg gaggcgggcg ccgagctcca cgtccggctc ctgcactccc
     2581 gagtcgaggt cccgggcgcc accctgctcg agcgcctggc tgcccggcgc ccggccctgc
     2641 gcgagccgcg gcccgtcgtc gtcggcgcct tccctgcggg ccggccatgg ccaccggtca
     2701 ccgaccccgt acgccggcag cgccgggtgc ggcggtgggc ggccatcgcc atcgccgtcg
     2761 ccggagtgct ggacctgctg tcgggcc
//
LOCUS       DASWGG010000424         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2224198, whole genome shotgun sequence.
ACCESSION   DASWGG010000424 DASWGG010000000
VERSION     DASWGG010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2224198"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1573)
                     /locus_tag="VF954_07850"
     CDS             complement(<1..>1573)
                     /locus_tag="VF954_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9531036.1"
                     /translation="RPGELALALPVVLDPDSAEASGLQLAAQACEVVLSNAERIHAME
                     EVVAELSALENVATEILSVREVDQVLLSITRKAITLLEADMSGVFLIEGENLVMRSLM
                     GHRSVETARLRMKTGQGVAGRVIETGAPCKVDSYLESETITHDFDPLARVENTRSALA
                     APLKVHGEVIGVLEVWRRRSSLFTDRHVHRLAALANLAAIAIENARLYDHQQESMRQL
                     AKAQDSLARQLHAQKEAGTMQRALIQLVLEGEGLPAIVRTMAAQVGGQVGVFGSDFEP
                     LAVYPRDAAIDRMVAKMRRVAQGPSPRAGSITTVADDDRWITLQPIAAGRDALGWFCL
                     LSEQAPTTNVEIAIGEAVLSCALSQLEQRAADQARSEAQDEILWDLLEGSLDHRRAAM
                     ARANRLHIDLARPNRVVHALIEEFDEAVKSEGWDTARVERLRRELRGMTQRVLSEHGA
                     GELLSIRANSLVAVVPCSEPVEVRDLVRALRPEIQRMVPGVNVVWGISGAHENPLDYK
                     SANSEAHIALRAARRM"
BASE COUNT          205 a          551 c          584 g          233 t
ORIGIN      
        1 ccatccgccg ggccgcccgg agggcgatgt gggcctcgga gttggccgac ttgtagtcga
       61 gcgggttctc gtgggcgccg ctgatgcccc acacgacgtt gacgccgggc accatgcgct
      121 ggatctccgg ccgcagcgcc cggaccaggt cccgcacctc gaccggctcg ctgcagggga
      181 cgacggccac cagcgagttg gcccggatgc tgagcagctc tcccgcaccg tgctcggaga
      241 ggacccgctg cgtcatgccc cgcagctcgc ggcgcagccg ctccacccgg gcggtgtccc
      301 acccttcgga cttcaccgcc tcgtcgaact cctcgatgag ggcgtggacg acgcggttgg
      361 gccgggccag gtcgatgtgg agccggttgg cccgggccat cgccgcccgg cggtggtcaa
      421 gggagccctc gaggaggtcc cagaggatct cgtcctgggc ttcggaccgg gcctggtcgg
      481 cggcgcgctg ctccagctgg ctcagggcgc aggacagcac ggcctcgccg atggcgatct
      541 ccacgttggt ggtgggcgcc tgctccgaca ggaggcagaa ccagccgagg gcgtcgcggc
      601 cggcggcgat cggctgcagg gtgatccacc ggtcgtcgtc ggccaccgtg gtgatgctcc
      661 cggcccgggg ggacggccct tgcgccaccc ggcgcatctt cgccaccatg cggtcgatgg
      721 cggcgtcgcg ggggtagacg gccaggggct cgaagtcgct gccgaagacc ccgacctgac
      781 cgccgacctg agcggccatg gtccgcacga tggccggcag cccctcgccc tccagcacca
      841 gctggatgag cgcccgctgc atcgtccccg cctccttctg ggcgtggagc tggcgggcca
      901 gcgaatcctg ggccttggcc agctggcgca tgctctcctg ctggtggtcg tagaggcggg
      961 cgttctcgat ggcgatggcg gccaggttgg ccaaggccgc caaccggtgg acgtgccggt
     1021 cggtgaacag cgagctccgc cgccgccaga cctcgagcac tccgatgacc tcgccgtgga
     1081 ccttgagcgg cgcggccagc gccgagcggg tgttctcgac ccgggccagc gggtcgaagt
     1141 cgtgggtgat cgtctcgctc tcgagatagc tgtcgacctt gcagggcgcc ccggtctcga
     1201 tgacccggcc ggccacgccc tggccggtct tcatgcggag ccgggcggtc tccacgctgc
     1261 ggtggcccat gaggctgcgc atcacgaggt tctcgccctc gatgaggaag acccccgaca
     1321 tgtcggcctc gaggagggtg atggccttgc gggtgatcga caggaggacc tggtcgacct
     1381 cgcggacgga gaggatctcg gtggccacgt tctccagggc ggagagctcg gccacgacct
     1441 cctccatggc gtggatccgc tcggcgttgg acaggacgac ctcgcaggcc tgggcggcca
     1501 gctggaggcc ggaggcttcg gcggagtccg gatcgaggac caccggcagg gccagggcca
     1561 gctccccggg ccg
//
LOCUS       DASWGG010000425         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2471484, whole genome shotgun sequence.
ACCESSION   DASWGG010000425 DASWGG010000000
VERSION     DASWGG010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2471484"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VF954_07855"
     CDS             <1..1034
                     /locus_tag="VF954_07855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX9531037.1"
                     /translation="LHELEALARAAMDPEAFDFVAGGAGDEVTLRENAAAFARRRLWP
                     RVLTGVVGVDTSATMLGAPVSMPVGLAPAALQGLAHPDGEIAAARAAASAGAVYCLST
                     VSTHPIEAVGAATDGAWWFQLYIQKDRALTEDLVRRAEAAGCRAVVLTVDLPVTGYRD
                     RELRQGFAGHWDGLGNFDRPGLGDLSLRELIATSFDPLLTWDDVGWVRSLSALPLVVK
                     GILTPEDAELAAAYDASAVVVSNHGGRQLDRCPASLDVLEEVVTAVGGRLEVYLDGGV
                     RRGGDVVIALALGARGVFIGRPYLYALAAGGEDGVRRALELLSVEMRNAMTLLGTPDL
                     GSVTRSQVV"
     gene            complement(1045..>2361)
                     /locus_tag="VF954_07860"
     CDS             complement(1045..>2361)
                     /locus_tag="VF954_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9531038.1"
                     /translation="RAVAGRLHAAGLRRRDRVILSAAPSVALVKAYVAALRLGLVVVP
                     LNPGYREREVTHVVRDAEPPAAIVDDAERGAWIRRAAGNALILGPDVDLPDGNVADLD
                     DLDAGDPALICYTSGTTGSPKGAVLAHGNLLASAQAIRLAWRWSDNDRLVLALPLFHV
                     HGLCVGLHGTLLAGGSAVLQPRFEPDAVLSAVRRHEATLFFGVPTMYHRLARSPRVTE
                     LGRLRLCVSGSAPLAPALWERIRWESGQHVVERYGTTETLMNTSNPYDGERRPGSVGM
                     ALPGVQVRLDGEVPGEILVAGPNVFSGYWRNAQATADAFDSQGWFRTGDIGRLGADGY
                     LRIEGRVQEVIISGGFNVYPREVEEVLLTHPGVAEVSVVGTPSEEWGETVTAFVVPVD
                     QSPEPEALLAFAAERLAAYKRPRTVRFLNALPRNATGKVVRRELPA"
BASE COUNT          323 a          834 c          882 g          322 t
ORIGIN      
        1 gcctgcacga gctcgaggcc ctcgcccggg ccgccatgga tcccgaggct ttcgacttcg
       61 tcgccggcgg cgccggcgac gaggtgacac tgcgggagaa cgcggcggcc ttcgcccggc
      121 gccggctgtg gccccgggtg ctcacgggtg tcgtcggggt ggacacgtcg gccacgatgc
      181 tgggagcgcc agtatcgatg ccggtcgggc tggctcccgc agcgctgcag gggctggcgc
      241 acccggacgg ggagatcgcc gcggcccggg cggcggcgag cgcaggcgcc gtctactgcc
      301 tctcgacggt gtcgactcac cccatcgaag cggtgggagc agcgaccgac ggcgcctggt
      361 ggttccagct ctacatccag aaggaccggg cgctgaccga agacctcgtg cggcgggcgg
      421 aagcggccgg ttgccgggcg gtcgtgctca cggtcgacct tcccgtgacc ggctaccgcg
      481 accgcgagct gcgacagggc ttcgcgggcc actgggacgg gctgggcaac ttcgaccggc
      541 cgggacttgg cgacctctct cttcgggagc tgatcgcgac atccttcgat ccgctgctga
      601 cctgggacga cgtcggctgg gttcgctctc tttccgccct accgctggtg gtcaagggca
      661 tcctgacccc cgaagacgcc gagctcgcgg ccgcatatga cgcctcggcg gtggtggtct
      721 ccaaccacgg cggcaggcag ctcgaccgct gccccgccag cctggacgtc ctggaggagg
      781 tggtcaccgc cgtcgggggg cgcctggagg tctacctgga cggcggggtg cgccgaggcg
      841 gggacgtggt gatcgcgctg gcccttgggg cgcggggcgt gttcatcggc cggccctacc
      901 tgtacgcgct cgcggccggc ggcgaggacg gggtccggcg cgcgctcgag ctgctgtcgg
      961 tggagatgcg caatgccatg acgctgctcg gcacccccga cctcgggtcg gtcacccgct
     1021 cacaggtcgt gtgactggcg cccatcaggc aggtagctcg cggcggacga ccttgccggt
     1081 ggcgttgcgg ggcagggcgt tcaggaagcg gaccgtgcgg gggcgcttgt aggcggcgag
     1141 gcgctcggct gcaaacgcca ggagcgcctc gggttcggga gactggtcca ccggaaccac
     1201 gaacgcggtc acggtctcac cccactcctc ggacggcgtg cccaccacgg agacctccgc
     1261 gacgccggga tgggtgagga ggacctcctc gacctcgcgc ggatagacgt tgaagccccc
     1321 ggagatgatc acctcctgga cccgtccctc gatccggagg tagccgtccg ccccgagacg
     1381 cccgatgtcg cccgttcgga accagccctg ggagtcgaac gcatcagcgg tcgcctgcgc
     1441 gtttcgccag tagcccgaga agacgttcgg gcccgcgacg aggatctcgc cggggacctc
     1501 cccgtccagg cggacctgga ccccggggag tgccatcccg acgctgcccg gcctccgctc
     1561 gccgtcgtag gggttcgagg tgttcatcag ggtctccgtc gtgccgtagc gctcgaccac
     1621 gtgctggccc gactcccagc gaatccgctc ccacagggcc ggggcgagcg gagccgatcc
     1681 cgacacgcag agccggaggc ggccaagctc cgtcacgcgg ggggatcgag caaggcggtg
     1741 gtacatcgtc ggcacgccga agaacagggt ggcctcgtga cgtcgcaccg ccgacagcac
     1801 ggcgtccggc tcgaagcggg gctggagcac cgccgagccg ccggccagca gcgtcccgtg
     1861 gagccccacg cacagcccgt gcacgtggaa cagcggcagg gcgagcacca ggcggtcgtt
     1921 gtcgctccac cgccaggcca accggatcgc ctgcgcgctg gcgagcaggt tgccgtgggc
     1981 caggaccgcc cccttcggcg accccgtcgt cccggaggtg tagcagatga gggccgggtc
     2041 gccggcgtcg aggtcgtcca ggtcggcgac gttcccatcg ggcagatcga cgtccggacc
     2101 gaggatgagt gcgtttccgg cggcccgacg gatccaggct ccccgctcgg catcgtccac
     2161 gatcgccgca ggaggctcgg cgtcccggac gacgtgggtg acctcccgct cccggtaccc
     2221 cgggttgagc ggcacgacca cgaggccgag gcgcagcgcc gcgacgtagg ccttgaccag
     2281 cgcgacggag ggtgcggcgc tcaagatcac ccggtctcgg cggcggaggc cggcggcgtg
     2341 caggcgcccc gccacggcgc g
//
LOCUS       DASWGG010000426         2644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2471616, whole genome shotgun sequence.
ACCESSION   DASWGG010000426 DASWGG010000000
VERSION     DASWGG010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2471616"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VF954_07865"
     CDS             complement(<1..786)
                     /locus_tag="VF954_07865"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEX9531039.1"
                     /translation="MVSELFDAAAWRPVDQFGGQDITYHRAVDQGTVRIAFNRPEVRN
                     AFRPETVDELYQALDHARMSTDVGCIILTGNGPSPRDGGWAFCSGGDQRIRGRDGYQY
                     NAGEDAAGPVDPARTGRLHILEVQRLIRFMPKVVLCVVPGWAAGGGHSLHVVCDLTLA
                     SREHARFKQTDLDVASFDGGFGSAYLARQVGQKLAREIFFLGREYDADEAHRMGMVNA
                     VVPHDRLEQVALDWARTVNERSPMATRMLKLALNAVDDGLVGQQ"
     gene            complement(767..>2644)
                     /gene="menD"
                     /locus_tag="VF954_07870"
     CDS             complement(767..>2644)
                     /gene="menD"
                     /locus_tag="VF954_07870"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEX9531040.1"
                     /translation="GAVAGDSASLHCVTTLRGDVTEANLLTQWARLLLGSLADAGVRH
                     VVLSPGSRSTPLMIAAERDSRLVCHDSIDERAAAFFALGQARVTGEPSLVISTSGTAP
                     AHYLPAVVEASLSYLPLVVLTADRPLELQDCGASQTTDQIKLMGDHVRRFYELGSPDP
                     APSALAGVRRLAAQAVHFSRFPVPGPVHLNARARKPLEPQAATTGDERDLEARVAGLL
                     SRPVARASLPTADPDPDGIRALAERCRSVERGLIVCGPAPADHVALRPAVRELAASTG
                     FPLLAEATSQLRFDAASTPGLTRCDAFDALLRSPHFQATHRPELVVQVGAAPVSSGFE
                     QYLAAHDGVDRVVIAPHGWQDAGGATALILGDPQRALRGVLSALDARRPGPSPWAQLF
                     AGADRLAWEVVEAEMDGGDLTEGRAVRTVVEGLPTGSLLMVGNSLAVREVDTFCPAGT
                     ADVTVLSQRGASGIDGLVAGASGGASVQGQPVTLLLGDISLLHDLTGLALAADAAVAL
                     AVVVLNNGGGRIFEQLPFATAPSVPAGAMDHVTTPHRFELEHAALLFGHRYAAARTAE
                     ELAKALEAALGEPGCTLIEVPLPPHGAAEQHGRLFAKVDAAVRSRSTDTGEEGVGIGA
                     V"
BASE COUNT          388 a          931 c          990 g          335 t
ORIGIN      
        1 ctgctggccg acgaggccgt cgtcgacggc gttgagggcc agcttgagca tccgggtcgc
       61 catggggctc ctctcgttga cggtgcgggc ccagtccagc gcgacctgct cgagccggtc
      121 gtggggcacc acggcgttga ccatgcccat gcggtgcgcc tcgtcggcgt cgtactcccg
      181 cccgaggaag aagatctcgc gggcgagctt ctggcccacc tggcgagcga ggtaggcgga
      241 gccgaagccc ccgtcgaagc tggccacgtc gaggtccgtc tgcttgaacc gggcgtgctc
      301 gcggctggcg agcgtcagat cgcacacaac gtggaggctg tggccaccgc cggccgccca
      361 ccccggaacg acgcagagca cgaccttggg catgaaacgg atgaggcgct gcacctccag
      421 gatgtggagc cggccggtcc gggccgggtc gacggggccc gccgcgtcct cgcccgcgtt
      481 gtactggtag ccgtcccggc cccggatgcg ctgatcgccg ccggagcaga acgcccaccc
      541 gccgtcccgg ggggacgggc cgttgcccgt gaggatgatg catcccacgt cggtcgacat
      601 gcgggcgtgg tccagcgcct ggtagagctc gtccacggtc tcgggccgaa aggcgttccg
      661 gacctcggga cggttgaacg cgatcctcac ggtgccctgg tcgacggcgc ggtgataggt
      721 gatgtcctgg ccgccgaact ggtcgaccgg ccgccacgcg gcggcatcaa acagctccga
      781 taccaacgcc ttcctcccct gtgtcggtgg accgtgatcg gacggcggcg tccaccttgg
      841 cgaaaagccg gccgtgctgc tctgcggcgc cgtgcggagg caggggcacc tcgatcaggg
      901 tgcaacccgg ctcaccgagc gccgcctcca gggccttggc cagctcctca gcggtcctgg
      961 cggcggcata gcggtgcccg aacaggagcg cggcgtgctc cagctcgaag cgatgcgggg
     1021 tggtgacgtg atccatcgcc cccgccggca cggaaggggc cgtagcgaac ggcagctgtt
     1081 cgaagatgcg gccgccgccg ttgttgagca cgacgacggc gagggcaacg gccgcgtcgg
     1141 ccgccaaggc cagaccggtc agatcgtgca gcaggctgat gtcgcccaac aacagcgtga
     1201 ccggctgacc ctggacagac gctccgcccg acgcgccggc caccaggccg tcgatgccgc
     1261 tggccccgcg ctggctgagc accgtcacgt ccgccgtgcc ggccgggcag aaggtgtcga
     1321 cctcccgcac cgccaggctg ttgccgacca tgagcagaga cccggtcggc agaccctcga
     1381 ccacggtgcg aacggcacga ccttccgtca ggtcgccgcc atccatctcg gcctcgacga
     1441 cctcccaggc caggcgatcg gcgccggcaa agagctgcgc ccagggcgac ggcccgggcc
     1501 ggcgcgcgtc aagagctgac agaactcccc tcagcgcgcg ctgcggatcg ccaaggatga
     1561 gggcggtggc gccgccggcg tcctgccacc catggggcgc gatgacgacg cggtcgaccc
     1621 cgtcgtgcgc ggccaggtac tgctcgaagc ccgaggacac cggggcggcg cccacctgga
     1681 ccacgagctc cggtcggtgg gtggcctgga aatggggcga gcggagcagg gcgtcgaagg
     1741 cgtcgcatcg cgtcagaccc ggagtgctcg cggcgtcgaa ccggagctgg ctggtagcct
     1801 cggccagcag cgggaagccg gtggacgcgg ccagctcccg cacggcgggg cgcagggcga
     1861 catggtccgc aggcgccggg ccgcagacga tcagtccccg ctccacgctc cggcaacgct
     1921 cggccagggc ccggatcccg tccgggtcgg gatcggccgt cggcaggctc gccctggcca
     1981 cggggcgcga gagcaggcct gcgacgcggg cctccagatc gcgctcgtcg ccggtcgtgg
     2041 ccgcctgcgg ctcgagcggc ttgcgggccc gggcgttgag atgcaccggg cccggcacag
     2101 ggaagcggct gaagtggacc gcctgggcgg cgagacggcg cacgccggcg agggcggagg
     2161 gcgccgggtc cgggctcccc agctcgtaga agcgccggac gtggtcgccc atcagcttga
     2221 tctgatcggt cgtctgggac gcaccacagt cctggagctc gagggggcga tcggcggtga
     2281 gcacgaccag cggcaggtac gagaggctgg cctcgacgac cgcgggcagg tagtgcgccg
     2341 gtgccgtgcc cgaggtgctg atcaccaacg agggctcgcc ggtgacccgg gcctgcccca
     2401 gggcgaagaa cgccgccgcc cgctcgtcga tcgagtcgtg gcagaccagc cggctgtccc
     2461 gctccgccgc gatcatgagc ggggtcgagc gcgagcccgg gctgagcacg acgtggcgga
     2521 ccccggcatc ggccaggctg ccaagcagga gccgggccca ctgggtgagc aggttggctt
     2581 cggtcacatc gccgcgcagt gtagtgacgc agtgtagtga cgccgagtcg ccggccacgg
     2641 cgcc
//
LOCUS       DASWGG010000427         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_624162, whole genome shotgun sequence.
ACCESSION   DASWGG010000427 DASWGG010000000
VERSION     DASWGG010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624162"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..>2392)
                     /locus_tag="VF954_07875"
     CDS             complement(163..>2392)
                     /locus_tag="VF954_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9531041.1"
                     /translation="LTLGGTYVDDVRLEGAAWVTYVRSTMAHARITGVDTAEAANGPG
                     VIAVFTAGDVDLAPIAPPMPFINQAMTHPWLATGKVRFVGEPVAAVVTEQRYQGPDAA
                     ELVFVDYEPLPAVVDPEEAVGSGTHLFEDVGSNIACDVSAMTGFTEDPDFFEGCDVVV
                     RGRFVNQRLAPCPMEVRSAAAAWEGDRLTQWASTQAPNTLRDGLAAVYGLDPANLRVI
                     APDVGGGFGAKVGVYTEEGLLGWIAKRVGRPVRWTETRTESMTTLGHGRGQIQYVELG
                     GTTDGKVLAFRNRMVQDSGAYPAIAAFLPILGRMMATGVYDIPKAAAGGAAVVTNTTS
                     IVAYRGAGRPEAAAAIERAMDMFAGEIGMDPAEVRRRNLVPKDKFPYTTATGTVYDTG
                     DYGRALDLVLAGAGYDDLRRQQAERRASGDRNQMGIGMSVYVEITNPLPGGEFGSVEV
                     TPDGKAKIRAGTFSHGQGHATTFAMIVADRLGIALEDIELIQGDTDQVPTGQGTMGSR
                     SLQTGGVAVAEASTQIIDRARQLAADLLEASADDIVLDPAGGRFHVAGTPAVGKTWAE
                     IAGSTGDSGLRVDVDFMPGGPTFPFGAHVAVVEVDTETGQVRLTRMITVDDSGRMLNP
                     LIMEGQRHGGIAQGVAQALMEEFRYDPDGNPVTANLADYAFISAMEVPSYELIPMETP
                     TPLNELGAKGIGESGTIGSTPAVQNAVVDALSHLGVKHIDMPLSPERVWSALNAAGSA
                     GG"
BASE COUNT          305 a          875 c          853 g          359 t
ORIGIN      
        1 acgcggccac cgcgacggcg gtcgatcccg ccgcgccgcg ccatagcagt gccctccggt
       61 tgccccgccg tgccacgctc ctacggtgcc agacgcacgc gggctccgcc atggtccatc
      121 tgtgtcattc ccgccccgcc acgagccccc gccgcgacat cctcacccgc cggcggaccc
      181 cgcggcgttc agcgcgctcc acacccgctc cgggctgagc ggcatgtcga tgtgcttcac
      241 gcccaggtgg ctcagggcgt ccacgaccgc gttctggacg gccggagtgg agccgatggt
      301 gcccgactcg ccgatgccct tggccccgag ctcgttcagc ggcgtcggcg tctccatcgg
      361 gatcagctcg tagctgggga cctccatggc cgatatgaag gcgtagtcgg cgagattggc
      421 cgtcaccggg ttgccgtcgg ggtcgtaccg gaactcctcc atgagggcct gggccacccc
      481 ctgggcgatg cccccgtggc gctggccctc catgatcagg gggttgagca tgcgcccgga
      541 gtcgtcgacg gtgatcatgc gggtgagccg cacctggccg gtctcggtgt ccacctccac
      601 cacggccacg tgggccccga acgggaaggt cgggccgccc ggcatgaagt cgacgtcgac
      661 gcgcaggccg ctgtcacccg tggagccggc gatctcggcc caggtcttgc cgaccgcggg
      721 agtcccggcc acgtggaacc ggccgccggc cgggtccagc acgatgtcgt ccgcgctggc
      781 ctcgagcagg tcggcggcca gctgccgggc ccggtcgatg atctgggtgg acgcctcggc
      841 gacggcgacg ccgccggtct gcagcgaccg cgagcccatg gtgccctgcc cggtcgggac
      901 ctggtcggtg tcgccctgga tgagctcgat gtcctccagc gcgatcccca ggcggtcggc
      961 cacgatcatg gcgaaggtgg tggcgtggcc ctgcccgtgc gagaaggtcc cggcccggat
     1021 cttggccttg ccgtcggggg tgacctccac cgacccgaac tccccgccgg ggagcgggtt
     1081 ggtgatctcc acgtacacgg acatcccgat gcccatctgg ttgcggtcgc cgctggcccg
     1141 gcgctcggcc tgctggcgcc gtaggtcgtc gtagccggcg ccggccagca ccaggtcgag
     1201 agcgcgtccg tagtcgccgg tgtcgtagac cgtgccggtc gccgtggtgt acgggaactt
     1261 gtccttgggc accaggttgc gccggcggac ctccgcgggg tccatgccga tctcgccggc
     1321 gaacatgtcc atggcccgct cgatggccgc ggccgcctcg ggccggcccg cgccccggta
     1381 tgccacgatc gaggtggtgt tggtgaccac cgcggctccg cctgcggccg ccttcgggat
     1441 gtcgtagacg cccgtggcca tcatgcgccc caggatcggc aggaacgcgg cgatcgccgg
     1501 gtaggcgcct gagtcctgca ccatccggtt gcggaaggcc aggaccttgc cgtccgtcgt
     1561 gccgcccagc tcgacgtact ggatctgccc ccgcccgtgg ccgagcgtcg tcatgctctc
     1621 ggtccgggtc tcggtccacc gcaccggccg gcccacccgc ttggcgatcc agccgagcag
     1681 gccctcctcg gtgtagacgc cgaccttcgc tccgaacccg ccgcccacgt cgggagcgat
     1741 gacacggagg ttggcggggt cgaggccgta gacggctgcc aacccgtcgc ggagggtgtt
     1801 gggagcctgg gtcgaggccc actgggtcag gcggtcgccc tcccatgccg ccgccgccga
     1861 ccgcacctcc atggggcaag gggccagccg ctggttcacg aagcgccccc ggaccaccac
     1921 gtcgcagccc tcgaagaagt cggggtcctc ggtgaagccg gtcatggcgg agacgtcgca
     1981 ggcgatgttg gagccgacgt cctcgaacag gtgggtgccg gacccgaccg cctcctcggg
     2041 gtcgaccacg gcggggagcg gctcgtagtc gacgaagacc agctcggccg cgtccggccc
     2101 ctgatagcgc tgctccgtca cgaccgcggc cacgggctcg ccgacgaagc ggaccttccc
     2161 cgtggccagc cagggatggg tcatggcctg gttgatgaag ggcatcggcg gcgcgatggg
     2221 cgcgagatcg acgtcacccg cggtgaacac ggcgatgacg ccggggccgt tcgcagcctc
     2281 cgcggtgtcg acgcctgtga tccgggcgtg ggccatggtc gatcggacgt aggtcaccca
     2341 cgccgccccc tccagcctga cgtcgtcgac gtaggtgccg ccgagggtga gg
//
LOCUS       DASWGG010000428         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2232395, whole genome shotgun sequence.
ACCESSION   DASWGG010000428 DASWGG010000000
VERSION     DASWGG010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232395"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VF954_07880"
     CDS             complement(<1..255)
                     /locus_tag="VF954_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9531042.1"
                     /translation="MLTVDYGRLGLRPGDRLLDLGCGAGRHAYEALRQGGRVVALDSD
                     AAELKDVAAVLEALLEEGPAGGTPTATGAAAVNGDAVGLPF"
     gene            complement(260..1513)
                     /locus_tag="VF954_07885"
     CDS             complement(260..1513)
                     /locus_tag="VF954_07885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9531043.1"
                     /translation="MPAAPERLRIAYLAYRGNPHSGGQGVYTRYLTRELVGLGHHVTV
                     FGGQPYPVLDDGVEMVPIPSLDLYRQPDPFRIPRLGEFHSAVDALEFALMCTAGFPEP
                     LTFSLRARQVLAGRRGDFDLVHDNQCLGYGLLGMVRDGWPVLATLHHPITVDRDLDLA
                     HAATRRRRLTLRRWYGFLAMQSRVAARLPRVVTVSQSSCGDISTQMGVPASRLRVVPV
                     GVDHTLFRPIPGVARVPGRIMTTASSDVPLKGLVPLLEAVAKVRTERPVSVVVIGRPR
                     AGSDVGTTLDRLGLADAVRFVHGVPDTEIVRLYAEAEVAVVPSLYEGFSLPAVEAMAC
                     GVALVATTGGALPEVVGPDGETGLLVPPGDPGALASAILRALSDPCLRARVGAAGRER
                     VLERFTWRATAVGTVEQYRQLLEGG"
     gene            complement(1570..2865)
                     /locus_tag="VF954_07890"
     CDS             complement(1570..2865)
                     /locus_tag="VF954_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX9531044.1"
                     /translation="MSGEAAPPGDRAGRVVAHLPGRRLRQFVSYLALAGPGLIAANAG
                     NDAGGIATYASAGSQYRYRTLFFMVLVTVGLVVVQEMAARLGAYTGKGLASLIREQFS
                     VRLAGLAVVALLLSNTGLVVSEFAGIGASLELFHVSRYVSIPIAAVAIWALVLFGSYR
                     YAERVFLVLSLVFFGYPLAVFFGHPDWHKVAAQTVWPHFVGSKDFIFLGVALIGTTIS
                     PYMQLYGAAGVVDRGIGPDEYRFERIDAVGGALFADLVSMFIIIATAATIGGRGPLSS
                     AREAAEALKPVAGAGAEVLFAMGLLGASALAAAVVPLSTSYAVAEAIGVERSVSRRFS
                     EAPVFLGLFTAQVLIGALVALTPVNLIRLLIGTQVLQGIITPVILVFILVLANRRSVL
                     GDAVNGPVFRAVAALSIAAVSGLSVVLLAQTALGWLGLG"
BASE COUNT          473 a         1015 c         1149 g          305 t
ORIGIN      
        1 gaagggcagg cccacggcgt cgccgttgac cgccgccgcc ccggtggcgg tgggggtgcc
       61 gcccgccggg ccctcctcca gcagggcctc gagcacggcc gccacgtcct tgagctcggc
      121 cgcgtcggag tccagggcga ccacgcgccc gccctgccgc agcgcctcgt aggcgtggcg
      181 gccggcacca cagcccaggt cgaggagccg atcgccgggg cgcagcccga ggcgcccgta
      241 gtcgacggtg agcatcgggc tacccgccct cgagcagctg gcggtactgc tcgaccgtgc
      301 cgacggcggt ggcgcgccag gtgaagcgct cgaggacccg ctcccggccg gcggcgccga
      361 cccgcgcccg caagcaggga tcgctgaggg cccggaggat ggcggatgcg agggcgcccg
      421 ggtcgcccgg cggcaccagc aggccggtct ccccgtcggg gccgacgacc tccgggaggg
      481 cgccgcccgt cgtggccacg agggccacgc cgcaggccat ggcctcgacg gcagggaggg
      541 agaagccctc gtacagcgac gggacgacgg ccacctcggc ctcggcgtag aggcgcacga
      601 tctcggtgtc gggcacgccg tgcacgaagc gcacggcatc ggcgagcccc aggcgatcga
      661 gcgtggtccc gacatcgctc cccgctcgag gtctgccgat gaccacgacc gacacgggcc
      721 gctcggtgcg caccttggcc accgcctcga gcaggggcac caggccctta agcgggacgt
      781 cgctgctggc ggtggtcatg atccgccccg gcacccgggc cacgcccgga atgggccgga
      841 acagggtgtg gtcgacgccg acggggacca cccgcagccg ggacgcgggc acgcccatct
      901 gggtggagat gtccccgcag gaggactggg acaccgtgac gacccggggc agccgggcgg
      961 ccacccggct ctgcatggcc aggaacccgt accagcggcg gagggtcagc cgccggcgcc
     1021 gggtggcggc gtgggccagg tcgaggtcgc ggtcgacggt gatggggtgg tgcagggtgg
     1081 cgagcacggg ccagccgtcg cgcaccatgc cgagcagccc gtaacccagg cactggttgt
     1141 cgtgcacgag gtcgaagtcg ccgcggcggc cggcgaggac ctggcgggcc cgcaggctga
     1201 aggtgagcgg ctcggggaac cccgcggtgc acatgagggc gaactcgagc gcgtcgacgg
     1261 cgctgtggaa ctcgccgagc ctggggatcc ggaacgggtc gggctgccgg tagaggtcca
     1321 ggctggggat cggcaccatc tcgacgccgt cgtcgagcac ggggtagggc tggcccccga
     1381 agacggtgac gtgatggccc agccccacca gctcgcgggt gaggtagcgg gtgtagacgc
     1441 cctggccgcc cgagtggggg ttgccccggt aggcgaggta ggcgatgcgg agccgctcgg
     1501 gggctgcggg catggcccga gagcctggcc gccaattgac cgcccggtca agatggctcg
     1561 ccggggccat cagccgagac cgagccatcc gagcgccgtc tgggcgagca gcaccacgct
     1621 caggcccgag acggcggcga tggagagggc tgccaccgcc cggaagacgg ggccgttgac
     1681 ggcgtcgccc agcacgctgc ggcggttggc gagcacgagg atgaaaacga ggatgaccgg
     1741 ggtgatgatg ccctggagca cctgggtgcc gatcagcagc cggatgaggt tgacgggggt
     1801 cagggccacg agcgcgccga tgagcacctg ggcggtgaac agcccgagga agaccggggc
     1861 ctcggagaac cggcgggaga cggagcgctc gacgccgatg gcttcggcga cggcatagga
     1921 ggtcgaaagg ggcaccaccg cggcggccag ggcggaggcc cccagcagcc ccatggcgaa
     1981 gagaacctcg gcgccggccc ccgcgaccgg cttgagcgct tcggccgcct cccgggcgct
     2041 cgagaggggg ccccggccgc cgatggtggc cgcggtggcg atgatgatga acatggacac
     2101 gaggtcggcg aacagcgccc cgcccacggc gtcgatgcgc tcgaagcggt actcgtcggg
     2161 cccgatgccg cggtcgacca caccggcggc cccgtagagc tgcatgtagg gcgagatcgt
     2221 cgtgccgatg agggccacgc ccaggaagat gaagtccttg gaccccacga aatggggcca
     2281 gacggtctgg gcggccacct tgtgccagtc cgggtggccg aagaacacgg cgagcggata
     2341 gccgaagaac accagcgaca gcacgaggaa gacccgctcg gcgtagcggt aggagccgaa
     2401 gagcaccagc gcccagatcg ccaccgcggc gatcggtatg gagacgtacc gggacacgtg
     2461 gaacagctcg agcgacgcgc cgatgccggc gaactcggac accaccaggc ccgtgttgga
     2521 cagcagcagg gcgacgaccg ccagccccgc caggcggacc gagaactgct cccggatcag
     2581 cgacgccagc cccttgcccg tgtaggcccc gaggcgggcg gccatctcct gaacgacgac
     2641 caggcccacg gtcaccagca ccatgaagaa cagcgtccgg taccggtact ggctgccggc
     2701 cgaggcatag gtggcgatcc cgccggcgtc gttgccggcg ttggcggcga tgaggcccgg
     2761 gccggccagc gccaggtagg acacgaactg gcgcagccgg cgcccgggca ggtgcgccac
     2821 gacccgcccg gcccggtccc cgggcggcgc cgcctccccg ctcactggag gagcccggtc
     2881 acgggaggag cccggtcact ggaggagcct ggggaagtgc atccggccgc gctcggggat
     2941 ga
//
LOCUS       DASWGG010000429         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2237325, whole genome shotgun sequence.
ACCESSION   DASWGG010000429 DASWGG010000000
VERSION     DASWGG010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237325"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VF954_07895"
     CDS             complement(<1..250)
                     /locus_tag="VF954_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531045.1"
                     /translation="MIVLLVACWLGFAAGTWVLIVGRTPSLAEDLAVGGAVQPAGWRG
                     LVEGVGNRMARLLARMGRASAVLEADLAVRGKTAGAFYG"
     gene            complement(247..1029)
                     /locus_tag="VF954_07900"
     CDS             complement(247..1029)
                     /locus_tag="VF954_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9531046.1"
                     /translation="MFLGPLVGLGVVQILRGGPFLPRLPRRLPKGLVIGLVAGVVLLL
                     VTGWPVLALAGGFLATQMLHSRAEAAEERRTLARRAELARVSSRLRDACLAGHGLPAA
                     VGIAAAAAGPQTREDMTALARAVREVGVGRAFTAWGERAPDPIFGVFARMVGEADRQG
                     SDALSSLLTRLATQTAKEVSAARETAAHQGGPRTTALVVAVVAVGLLLSVRFGSPSFA
                     AAYSDPTGQVMMAAAFVPIAVGYRAMARLGRGRHGLSWGGPR"
     gene            complement(1035..>2074)
                     /locus_tag="VF954_07905"
     CDS             complement(1035..>2074)
                     /locus_tag="VF954_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX9531047.1"
                     /translation="IQPVADDPTTTDIVITGPADGHVNRAGVWSRFDPGFASAEALEE
                     WARRLAERHGHHVDHASPEVDFAIARPRGRFHVVIPPIAGECAQVSIRCLRLVGEDLD
                     ALVRLGTLGNEAAACLLAAVAAKVNILVSGGGSSGKTTLTNALGRAIPAHERVVTIED
                     LAELALVDHLARCSALYTHRANIEGKGARSIEDLIPMALRMCADRILVGEIRASREAV
                     QVLDVMNTGHEGSITTIHANSAADALDRLVTLVGNAGWGYEFAVRRVAQTVAFVVHMR
                     KLADGRRVVETITEVDGAERTGVIRATDIFALVDGELVYRNRPREDRYLRLLEAGWQA
                     PIDTLEVGRWH"
BASE COUNT          302 a          748 c          767 g          257 t
ORIGIN      
        1 cgccgtaaaa ggccccggca gttttgcccc gcaccgccag gtccgcctcg aggacggccg
       61 acgcccggcc catccgggcc agcaggcgag ccatgcggtt ccccacgccc tcgacgaggc
      121 ctcgccagcc ggcgggctgc accgcaccgc cgacggccag atcctcggcg aggctcggcg
      181 tgcggccgac gatgagcacc cacgtgcccg cggcgaagcc gagccagcac gcgaccagga
      241 ggacgatcac cgcgggcctc cccagctcag gccgtggcgg ccccggccga gccgggccat
      301 ggcccggtac ccgacggcga tgggcacgaa ggccgcggcc atcatgacct gccctgtggg
      361 gtcgctgtag gcggctgcga aggacgggga accgaaccgg acgctcagca gcaggccgac
      421 cgcgacgacg gccacgacca gggccgtagt ccggggcccg ccctggtgtg ccgctgtctc
      481 tcgagccgcg gagacttcct tggccgtctg ggttgcgagc cgggtgagca gcgaggacag
      541 ggcatcgctg ccctggcggt cggcctcgcc caccatgcgg gcgaagacac cgaagatcgg
      601 atccggggcc cgctcgcccc aggcagtgaa ggcccggccg acgccgacct cccggaccgc
      661 ccgggccagg gccgtcatgt cctcgcgcgt ctgcggcccg gcggccgccg ctgcgatgcc
      721 gaccgcggcg ggcagcccat gccccgcgag gcaggcgtcg cgcagccggg aagagacccg
      781 ggctagctcg gcccggcggg cgagtgttcg gcgctcctct gccgcctcgg cgcgtgagtg
      841 cagcatctgg gtggccagga atccgccggc tagggcaagg acgggccagc cggtgaccaa
      901 gagcaggacc acgccggcca cgagtccgat cacgaggcct ttgggcagcc ggcggggaag
      961 gcgcggcagg aaaggaccac cccggaggat ctggacgacg ccgaggccga cgagtggccc
     1021 caggaacatg ccgatcaatg ccaccggcct acctccagtg tgtcgatcgg cgcttgccac
     1081 ccggcctcga gcaggcgcag gtagcggtcc tcccggggcc ggttgcgata gaccagctcg
     1141 ccgtccacca gggcgaagat gtcggtggcc cggatcacgc ccgtgcgctc ggctccgtcg
     1201 acctcggtga tcgtctcgac cacccgccgc ccgtcggcga gcttgcgcat gtggacgacg
     1261 aaggcgacgg tctgcgccac ccggcgcacc gcaaactcgt agccccagcc ggcgttgccc
     1321 accagcgtca ccagccggtc cagggcgtca gcggccgagt tggcgtggat ggtggtgatc
     1381 gacccctcgt ggcccgtgtt catgacgtcg agcacctgga cggcctcgcg gctggcccgg
     1441 atctcgccca cgaggatgcg gtcggcgcac atgcggaggg ccatggggat caggtcctcg
     1501 atgctgcggg ctcccttgcc ctcgatgttg gcccggtggg tatagagcgc cgagcagcgc
     1561 gccaggtggt ccaccagcgc cagctcggcc aggtcctcga tcgtcaccac gcgctcgtgg
     1621 gcggggatgg cccggccgag agcgttggtc agtgtggtct tgccggagga cccgccgccg
     1681 ctcacgagga tgttgacttt ggcagccacc gccgctagta ggcatgctgc ggcctcgttg
     1741 ccgagggtgc cgagccggac cagggcgtcg aggtcctccc ccaccaggcg caggcaccgg
     1801 atagagacct gggcgcactc cccagcgatc ggagggatca cgacgtggaa ccggccgcgg
     1861 ggccgggcga tggcgaagtc cacctcgggc gacgcgtggt cgacgtggtg gccgtggcgc
     1921 tcggcgagcc gccgagccca ctcctcgagg gcctcggcgc tggcgaaacc gggatcgaag
     1981 cgcgaccaca ccccggcccg gttgacgtgg ccgtcggccg ggccggtgat gacgatgtcg
     2041 gtcgtggtgg ggtcatccgc gacgggctgg atgc
//
LOCUS       DASWGG010000430         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2238918, whole genome shotgun sequence.
ACCESSION   DASWGG010000430 DASWGG010000000
VERSION     DASWGG010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2238918"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VF954_07910"
     CDS             <1..1299
                     /locus_tag="VF954_07910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX9531048.1"
                     /translation="TTVAARTDREREKGTNMALAEVFEAIAGPDAPVEFRAYDGSVCG
                     APGSDVKITIRSPVAVSYLAQAPGALGLARAYVSGHLDVDGDMYITLSRLSRAQETNM
                     SLAEKVKLLQSLGGPKVLVPRIPPPPQEVRINRRFLNGRMHSKGRDAHAISHHYDVSN
                     DFYRLVLGSTMTYSCARFESQGQSLDDAQQAKYELICRKLGLAPGQRLLDVGCGWGGM
                     VLHAAARHGIAALGVTLSRPQEELARKRAAESGLDGQVEIRLQDYRDLAGSYDAVSSI
                     GMFEHVGRARTAEYFTTLAALLRPGGRLLNHAISTPEGASFDRRSFVARYVFPDGELQ
                     DVGSVCLGMQKAGLEVRDVECLREHYALTLRHWAANLEANWDAAVALVGERRARVWRL
                     YMTGSAVSFEEGEISVHQVLGVKPDQGASGMALTRRAWDV"
     gene            1326..>2007
                     /locus_tag="VF954_07915"
     CDS             1326..>2007
                     /locus_tag="VF954_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX9531049.1"
                     /translation="MPITPADVARLPSPGGAIPVAVSFSPDDRTITFLHSPGRNLTRV
                     LEALDLGTGRRWTVEGTGDTEEDLAPEEKLRRERARDLGVGVTRYLRARHAETLLLPR
                     PDGLWARDTLEGESRLVVGAAEGALLDPQLSPGGTEVAFVREGELHVSDLAGGATRRI
                     TSGADPAAGLTHGLAEFVAQEEMGREHGYWWSRDGRWLAWTEVDESPVAPLRILHAGR
                     EEHGPAAEE"
BASE COUNT          290 a          715 c          738 g          264 t
ORIGIN      
        1 acgacagtgg cggcccggac agaccgggag cgcgagaaag gaacgaacat ggcgctggct
       61 gaggtattcg aggcgatcgc gggccccgac gccccagtgg aattccgggc gtacgacggg
      121 tcggtctgcg gcgcgcccgg ctcggacgtc aagatcacca tccggtcgcc cgtcgccgtg
      181 tcatacctgg cgcaggcccc gggagcgctc ggcctcgccc gggcctacgt ctccggccac
      241 ctcgacgtcg acggcgacat gtacatcacg ctgtctcgcc tgtcgcgggc gcaggagacg
      301 aacatgagcc tggccgagaa ggtcaagctg ctccagtcgc ttggcggccc taaggtcctg
      361 gtcccgcgca tcccgccgcc cccgcaggaa gtccggatca accgccggtt ccttaacggg
      421 cggatgcact ccaagggccg cgacgcccat gcgatctccc accactacga cgtgtccaac
      481 gacttctacc gcctggtgct gggctcgacc atgacgtact cgtgcgcccg gttcgagtcc
      541 caggggcagt cgttggacga cgcccagcag gccaagtacg agctgatctg ccgcaagctc
      601 ggcctggccc ccggacagag gctgctcgac gtcggctgcg gctggggcgg catggtcctg
      661 cacgccgcgg cccgtcacgg gatcgcggcg ctgggggtga cgctcagccg tccccaggag
      721 gagctggcgc gcaagcgggc cgccgagtcc ggcctcgacg gtcaggtcga gatccggctc
      781 caggactacc gggacctggc cgggtcctac gacgcggtgt ccagcatcgg gatgttcgag
      841 cacgtcggcc gggcacgcac cgctgagtac ttcacgaccc tcgcggccct gctgcgcccc
      901 ggcggccggc tgctcaacca cgccatctcc accccggagg gggccagctt cgaccgcagg
      961 tcgttcgtgg cccgctacgt gttccccgac ggcgagctcc aggacgtcgg atcggtctgc
     1021 ctgggaatgc agaaggcggg gctcgaggtg cgcgacgtcg agtgcctccg ggagcactat
     1081 gcgctgaccc tgcgccactg ggcggccaac ctcgaggcca actgggacgc cgccgtcgcc
     1141 ctcgtcggcg agcgccgcgc ccgggtgtgg cgcctgtaca tgaccgggtc ggccgtgagc
     1201 ttcgaggagg gggagatcag cgtgcaccag gtgctgggcg tcaagccgga ccagggcgcg
     1261 agcggcatgg ccctgacgcg gcgagcctgg gacgtctaga ccggtcccac ggacgagccc
     1321 gatcggtgcc catcacgcca gctgacgtcg cccgcctccc gtcgccggga ggcgccatcc
     1381 cggtcgcggt cagcttcagc cccgacgacc gcacgatcac cttcctccac tcgcccggcc
     1441 ggaacctgac ccgggttctg gaggccctcg acctcggcac cggccggcgc tggacggtcg
     1501 aaggtacggg tgacaccgag gaggacctgg cgcccgagga gaagctgcgc cgggagcggg
     1561 cccgggacct gggcgtgggc gtgacccgct acctccgggc caggcacgcc gagaccctcc
     1621 tgctgccccg ccccgacggg ctgtgggcgc gggacacgct cgagggcgag tcccgcctgg
     1681 tcgtcggcgc cgcggagggc gcgctcctgg acccgcagct gtccccgggc gggacagagg
     1741 tggccttcgt acgcgagggc gagctgcacg tgtccgacct ggcgggcggc gcgacgcgca
     1801 ggatcacgag cggggccgat ccagcggcag gccttacgca cgggctggcc gagttcgtgg
     1861 cccaggagga gatgggccgg gagcacggct actggtggtc acgggacggc aggtggctgg
     1921 catggacgga ggtcgacgag tcgccggtgg cgccgctgcg catcctgcac gccggccgcg
     1981 aggagcacgg tccggcggcc gaggagc
//
LOCUS       DASWGG010000431         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2239994, whole genome shotgun sequence.
ACCESSION   DASWGG010000431 DASWGG010000000
VERSION     DASWGG010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2239994"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2547
                     /locus_tag="VF954_07920"
     CDS             <1..2547
                     /locus_tag="VF954_07920"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX9531050.1"
                     /translation="SGLGASGVARAAIVIDDLHTLRGTPAEGALERLVECLPPGLVVL
                     AGTRVLPRFNLSRLRVSGDLLDLGADELRFRSWEVEELFRQVYGEPLRPEDFAQLARR
                     TAGWAAGLQLFHLATRGKPIEERTRAVTALANRHGLIHEYLVRNVLDELEPGLRGFVL
                     GAAALGRMSGELCDDLLEASGSARHLEELERRQLFTAAVEPAGWYRFHEVLRSHAEAA
                     FMQDAGEDPARRHRLRAAALLEKAGAWSEALQVYCRAGDWEAAARLVGLQADELVAGP
                     WVELLPEDMLRDSWVLLAAARRHRAAGRVAAAQADYARAERAFGTSSPGLVCRRERLA
                     LADWLAGAMYPSSTWSGLLRSALNRDPAVVADQARALGRGTGELAGAVADLLAGRVTD
                     ARSGLERLAERWPALSPVNLSARLALGVAATLSGQQVPGPELERLVEDCEDAALPWLA
                     EIARCLVPGSAEGTGSDPEPFGDALRRLLRAWTALAAGRRTDELPEPIVQRLGELAPV
                     AQAWALAVSALGAARASAPGAGERAHRAEQAARAVIAPGPLAIAQLAAGLVGGASAAR
                     SHEQGAQLARDLGLTVPGAPGPGALAPADRGAPCPPRVTVNCLGRFAVHVGAEAVDLG
                     LLKPRVRTLLRFLAMQGGRPVHREAIIEALWPDVAPDAATRNLQVAISSLRQVLDGAE
                     ARPDASAIRRDGDAYVLADQVSIDVAELASALARGRSRLRHEDPAGAQESFAQAVELY
                     GGELLPEEGPAEWVVAERDRLRVKIAEAAVEAARLALAHGDSGAAVSFCERALAVEPF
                     NDAVWQLLIAAHDAAESPASAARARARYRAVLDDLGVTERLDGVATGTDS"
     gene            2553..>2832
                     /locus_tag="VF954_07925"
     CDS             2553..>2832
                     /locus_tag="VF954_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX9531051.1"
                     /translation="MAPHIDSLSQHQAAPGQSLYANGSGFTGATGVQVNDQWADNYVV
                     DGDVSVTFTVPQLDAGTYWVLVHTADGASPCEDTAQQLTIADPTGAALS"
BASE COUNT          323 a         1102 c         1047 g          360 t
ORIGIN      
        1 tccggcctcg gcgcgagcgg cgtggcccgg gccgcgatcg tgatcgacga cctccacacc
       61 ctgcggggca ccccggccga gggcgccctc gagcggctgg tcgagtgcct cccgcccggc
      121 ctggtggtgc tggccgggac cagggtcctc ccccgcttca acctttcgcg cctgcgggtg
      181 tcgggggacc tgctcgatct tggggccgac gagctgcgct tccgctcgtg ggaggtggag
      241 gagctgttcc gccaggtgta cggcgagccc ctccgccccg aggacttcgc ccagctggcc
      301 cggcgcacgg cgggctgggc ggccggtctc cagctgttcc acctggccac ccggggcaag
      361 cccatcgagg agcggaccag ggcggtcacc gccctcgcca accggcacgg cctgatccac
      421 gagtacctgg tccgcaacgt gctcgacgag ctcgagcccg ggctgcgcgg cttcgtgctg
      481 ggcgccgcgg ccctcggccg catgagcggc gagctctgcg acgacctgct cgaggcctcg
      541 ggcagcgccc gccacctgga ggagctggag cggcgccagc tgttcaccgc cgccgtggag
      601 ccggccggct ggtaccggtt ccacgaggtc ctccgctccc acgccgaggc cgccttcatg
      661 caggacgccg gcgaggaccc cgcccgccgc caccgcctcc gggccgccgc cctcctcgag
      721 aaggcggggg cgtggtccga ggccctccag gtctactgcc gggccggcga ctgggaggcc
      781 gcggcccgcc tcgtcggcct gcaggccgac gagctggtgg ccgggccctg ggtcgagctg
      841 cttcccgagg acatgctgcg cgactcctgg gtcctgctgg cggccgcccg ccggcaccgg
      901 gccgcaggcc gcgtcgccgc cgcccaggcc gactacgcca gagccgagcg agcgttcgga
      961 acctcctcac cggggctcgt gtgccgccgg gagcgcctgg ccctggccga ctggctggcg
     1021 ggggccatgt acccgtcatc gacctggtcg gggctcctgc gcagcgcctt gaaccgcgat
     1081 cccgccgtgg tcgccgacca ggcccgggcg ctcggccggg gcaccggcga gctggccggc
     1141 gccgtcgccg acctgctggc gggccgggtc acggatgccc gctccggcct cgaacggctg
     1201 gccgagcgct ggcccgccct gtccccggtc aacctgtcgg cccggctggc cctcggcgtc
     1261 gccgccaccc tgagcggcca acaggtcccc ggtcccgagc tggaacgcct ggtcgaggac
     1321 tgcgaggacg cagccctacc gtggctggcc gagatcgccc ggtgcctggt gcccggctcg
     1381 gccgagggca ccggctccga tccggagccg ttcggtgacg ccctccgccg gctgctgcgg
     1441 gcgtggaccg ccctcgccgc gggccggcgc accgacgagc tgccggagcc gatcgtgcag
     1501 cgcctgggcg agctcgcacc cgtggcgcag gcctgggcgc tggcggtgtc ggcgctgggc
     1561 gcggcccggg cgagtgcccc cggagcgggg gaaagggccc atcgtgccga gcaggccgcc
     1621 cgggccgtca tcgccccggg cccgctggcc atcgcccagc tggcggccgg cctggtcggc
     1681 ggggcctcgg ccgcccgcag ccacgagcag ggcgcccagc tggcccgcga cctcggtctg
     1741 accgtgcccg gcgcgcccgg gcccggcgcg ctcgctcccg ctgatcgggg cgcgccatgc
     1801 ccgccgcggg tgacggtcaa ctgcctcgga aggttcgccg tgcacgtggg ggcggaggcg
     1861 gtggacctcg gcctgctcaa gccacgcgtg aggacgctgc tgcgcttcct ggccatgcag
     1921 ggcggccggc ccgtccaccg ggaggccatc atcgaggccc tctggcccga cgtcgcgccg
     1981 gacgcggcga cacgcaacct ccaggtggcc atctcctcgc tgcgccaggt gctcgacggg
     2041 gccgaggccc gacccgacgc ctcggccatc cggcgcgacg gcgacgccta cgtgctcgcc
     2101 gaccaggtgt ccatcgacgt cgccgagctg gcgtccgccc tggcccgggg ccggagccgc
     2161 ctgcgccacg aggatccggc cggggcgcag gagtccttcg cccaggcggt cgagctctac
     2221 gggggtgagc tgctgcccga ggaggggccg gccgaatggg tggtggccga acgcgaccgg
     2281 ctccgcgtca agatcgccga ggccgcggtg gaggccgccc gtctcgccct cgcccacggc
     2341 gacagcgggg cggcggtgtc cttctgcgag cgggctctgg cggtcgagcc cttcaacgac
     2401 gcggtctggc aactgctgat cgccgcccac gacgcggccg agtctccggc cagcgcggcc
     2461 cgggcccggg cccgctaccg cgccgtgctg gacgacctcg gcgtgaccga acgccttgac
     2521 ggcgtggcga ctggcaccga cagttgagtg ccatggctcc ccacatcgac agcttgtcac
     2581 agcaccaggc ggccccagga caatcgctct atgccaacgg cagcggcttc acaggggcca
     2641 ccggcgtcca ggtcaacgac cagtgggcgg acaactatgt ggtggacggc gacgtcagtg
     2701 tgaccttcac cgttccgcag ctggacgccg gcacgtactg ggtgctcgtc cacaccgctg
     2761 atggtgcctc accctgtgaa gacacggcgc aacagctcac catcgccgat ccgaccggcg
     2821 cagccctgtc gc
//
LOCUS       DASWGG010000432         3636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_635121, whole genome shotgun sequence.
ACCESSION   DASWGG010000432 DASWGG010000000
VERSION     DASWGG010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635121"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VF954_07930"
     CDS             <1..398
                     /locus_tag="VF954_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531052.1"
                     /translation="EQDIRREKASSNVCTNQTLIAVCCAIQLGWLGTAGLRELALRCA
                     RGTRYARQALLAVEGVTPVADAPVFREFAVRVPIDPGMVVERMAEEGFLAGIPLADEY
                     GEGLLVAVTERRTAEQIEGFAAALDKVLR"
     gene            395..1972
                     /gene="gcvPB"
                     /locus_tag="VF954_07935"
     CDS             395..1972
                     /gene="gcvPB"
                     /locus_tag="VF954_07935"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX9531053.1"
                     /translation="MSAPHAPNGRAASALRAPGGRASSAPIMGRDEEPTLFELSAPGR
                     SAASFRSTGVPEWAASELIPPGWLRDEPTPVAEVSERDLVAHFTRLTHRQYAVDLGAY
                     PLGSCTMKYNPKACDEAAALPGLADVHPAAPAPLTQGWMELLSDLDTILCRITGMHAA
                     TLQPAAGAAGELTGLLLMRAWHEDAGRQRRRVLIPDSAHGTNPASVTLGGYEVTQVPS
                     DDRGCVDVAALRERLDEDVAGIMLTNPNTLGLFEEDIVEIARSVHEVGGLLYYDGANL
                     NAILGIVRPGDMGFDIVHMNLHKTFAVPHGGGGPGAGPVAVSERLAPYLPGPRPVRGG
                     PDGAFTWERPARSIGRVHGWHGNALALARAYTYILLNGGDGLQRVAEAAVLNANWLRH
                     RLRGSYDIPYDRPNMHELVASVQGLKKRTGLRAVDVAKRLLEEGFHAPTTYFPLIVDE
                     ALMIEPTETESPETVAALGDALERIAREAEAEAEAGPEAGGVPAAQAAPRTTPVGRVD
                     EARAARTLIPTWDARGS"
     gene            complement(2159..2920)
                     /locus_tag="VF954_07940"
     CDS             complement(2159..2920)
                     /locus_tag="VF954_07940"
                     /inference="COORDINATES: protein motif:HMM:NF004855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX9531054.1"
                     /translation="MTELYLPTPPTFSSVEEERLHRKQRLAAAFRVFSKCGFDEGVAG
                     HITARDPELTDHFWVNPFGMHFGHIKVSDLILVNDRGDVVEGTRHVNRAAFAIHSQVH
                     AARPDVVAAAHAHSIYGKSFSSLGRKLDPITQDVCAFYGDHELFDDFTGVVLDTEEGK
                     RIAHALGEAKAVILRNHGLLTVGHSVDEAAWWFVTMERSCQAQLLAMSAGTPVHIDPE
                     MAALTATQIGSPTAGWFSFQPLYERIAAEQPDLFD"
     gene            3070..>3636
                     /locus_tag="VF954_07945"
     CDS             3070..>3636
                     /locus_tag="VF954_07945"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX9531055.1"
                     /translation="MADVPPQDPSYDPNAGAGYQQPPPPPPYGAPGAYGPPGAAGSAW
                     SGPPLAEWPQRFLAGLIDWGIGVVVLIGVVIVAAIVGAVSGTLGALVLVLGYLAVLAY
                     NYIALGYWNGAVGQTIGKKALGLKVVRMDDGQLLGGGAGIGRAFCHILDGICLIGFLF
                     PLWDPLKQTFADKIMKTVVIQVPKQPFKL"
BASE COUNT          525 a         1273 c         1315 g          523 t
ORIGIN      
        1 gggagcagga catccgccgc gagaaggcct cgtccaacgt ctgcaccaac cagaccttga
       61 tcgcggtgtg ttgcgccatc cagctgggct ggctcggcac ggccggcctg cgggagctgg
      121 ccctgcgctg cgcccggggc acccgctacg cgcgccaggc cctcctcgcc gtcgaggggg
      181 tcacgccggt cgccgatgcc ccggtcttcc gggagttcgc ggtgcgcgtg ccgatcgacc
      241 ccggcatggt ggtggagcga atggccgagg agggcttcct ggccgggata ccgctggcgg
      301 acgagtacgg ggaggggctg ctcgtggccg tgaccgagcg ccgcacggcc gagcagatcg
      361 aaggcttcgc cgccgccctc gacaaggtgc tccggtgagc gccccgcacg cgccgaacgg
      421 gagggcggcg agcgccctcc gtgcgccggg cgggagggcg tcgagcgccc ccatcatggg
      481 acgcgacgag gagccgacgc tgttcgagct gtccgcaccc ggccggtcgg cggcgtcgtt
      541 ccgcagcacg ggtgtgcccg agtgggcggc gtcggagctg atcccgccgg gctggttgcg
      601 cgacgagccc accccggtgg ccgaggtgtc cgagcgggat ctcgtggccc acttcacccg
      661 gctcacccac cggcagtacg cggtcgatct cggcgcctac ccgctcgggt cctgcaccat
      721 gaagtacaac cccaaggcgt gcgacgaggc cgccgccctc ccgggcctgg ccgacgtgca
      781 cccggccgcc ccggccccgc tgacccaggg ctggatggag ctgctcagcg acctggacac
      841 gatcctgtgc cgcataaccg ggatgcacgc ggcgacgctc cagcccgcgg ccggcgccgc
      901 cggcgagctg acgggcctgc tgctgatgcg cgcctggcac gaggacgcgg gccggcagcg
      961 gcgccgcgtg ctcatccccg actccgccca cggcaccaat cccgcgtccg tcaccctcgg
     1021 gggctacgag gtcacccagg tgccttccga cgaccggggc tgcgtcgacg tggcggccct
     1081 gcgcgagcgg ctcgacgaag acgtcgcggg gatcatgctg accaacccca acaccctcgg
     1141 cctgttcgag gaggacatcg tcgagatcgc ccggtcggtc cacgaggtcg gcggcctgct
     1201 gtactacgac ggcgccaacc tgaacgccat cctcgggatc gtccggcccg gcgacatggg
     1261 ctttgacatc gtgcacatga acctgcacaa gaccttcgcg gtgccccacg gcggcggggg
     1321 gccgggcgcc ggcccggtgg cggtgtcgga gcggctggcg ccgtacctgc ccgggccccg
     1381 gccggtgcgg gggggcccgg acggggcctt cacgtgggag cggccggccc gctccatcgg
     1441 ccgggtgcac ggctggcacg gcaacgccct ggcgctggcc cgggcctaca cctacatcct
     1501 gctcaacggc ggggacggcc tccagcgggt ggcggaggcg gcggtgctca acgccaactg
     1561 gctgcggcac cggctccgcg ggtcctacga catcccctac gaccggccca acatgcacga
     1621 gctggtggcc tcggtccagg ggctaaagaa gcgcaccggc ctccgggcgg tggacgtggc
     1681 caagcgcctg ctcgaggagg gcttccacgc gcccaccacg tacttcccgc tgatcgtcga
     1741 cgaggcgctc atgatcgagc ccaccgagac cgagagcccc gagaccgtcg ccgccctggg
     1801 cgacgctctg gagcgcatcg cccgcgaagc cgaggccgag gccgaggccg ggcccgaggc
     1861 cgggggagtg ccggcggccc aagccgcgcc ccgtaccacc ccggtcgggc gggtggacga
     1921 ggcgcgcgcc gccaggacgc tcattcccac atgggacgcc cggggcagct agttgccagc
     1981 gcgggccggg ttggacggtg gtacgagagc ccgccacggg cgtccgtcgc gccccccacc
     2041 cgtggttact ggggagataa cgcaacatga tgttgcgtta tctccccagc tattcggctc
     2101 tgcggcaagg gggcgggcca ccgtgtccac ttgaggcccg cgacgtcgtt cacgacaatc
     2161 agtcgaatag gtcgggctgc tcggcggcga tgcgctcgta gaggggctgg aagctgaacc
     2221 atccggccgt gggagagccg atctgagtgg cggtgagggc ggccatctcc gggtcgatgt
     2281 ggaccggcgt cccggcggac atggccagca gctgggcctg gcacgagcgc tccatggtca
     2341 cgaaccacca ggccgcctcg tccaccgagt ggcccaccgt gagcaggccg tggttgcgca
     2401 ggatcacggc tttggcctcg ccgagggcgt gggcgatgcg cttgccctcc tcggtgtcga
     2461 gcacgacgcc cgtgaagtcg tcgaagagct cgtggtcgcc gtagaaggcg cacacgtcct
     2521 gggttatggg gtcgagcttg cgcccgagcg aggagaagct cttgccgtag atcgagtggg
     2581 cgtgggcggc ggcgaccacg tcggggcgag cggcatggac ctgggagtgg atggcgaagg
     2641 cggcccggtt gacgtggcgc gtgccctcga ccacatcgcc gcggtcgttg accaggatca
     2701 ggtccgaaac cttgatgtgc ccgaagtgca tgccgaacgg gttgacccag aagtgatcgg
     2761 tcagctccgg gtcccgggcc gtgatgtggc cggcgacccc ctcgtcgaag ccacatttgg
     2821 agaagacccg gaaggcggcg gcgagccgtt gcttgcggtg gaggcgttcc tcctccaccg
     2881 aggagaacgt cgggggcgtg gggaggtaga gctcggtcac aggtagacct cctgatcggt
     2941 tcccagggcc aatcacaaag tctacggggg cgcggcgcgc ggcacgactg ccgcgacgat
     3001 cacggtttcg ccgtgttcaa cctgatacgt tccgcgggtc aacccgtctc acttgacagg
     3061 ggtcagccca tggcagacgt tccgccgcag gatccgagct acgacccgaa cgccggcgcc
     3121 ggttaccagc agccgccgcc acctccgcct tacggcgcgc ccggcgccta cggcccacct
     3181 ggagcggccg gctcggcatg gagcggccca ccgctcgctg agtggcccca gcggtttctg
     3241 gcaggcctga tcgactgggg catcggcgtc gtcgtgctca tcggggtggt catcgtggcc
     3301 gccatcgtgg gcgcagtgtc cggcacgctc ggcgcgctcg tgctcgttct cggctacttg
     3361 gccgttttgg cttacaacta catcgctctc gggtactgga acggcgcggt gggccagacc
     3421 atcggcaaga aggccctcgg cctgaaggtc gtccggatgg acgacgggca gctgctcggc
     3481 ggcggtgccg gcatcggccg cgccttctgc cacattctcg acggcatctg cctgatcggc
     3541 ttcctgttcc ccctgtggga ccccctcaag cagacctttg ccgacaagat catgaagacg
     3601 gtggtcatcc aggtaccaaa gcagcccttc aagctc
//
LOCUS       DASWGG010000433         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_312275, whole genome shotgun sequence.
ACCESSION   DASWGG010000433 DASWGG010000000
VERSION     DASWGG010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312275"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="VF954_07950"
     CDS             <1..1042
                     /locus_tag="VF954_07950"
                     /inference="COORDINATES: protein motif:HMM:NF020920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX9531056.1"
                     /translation="ETRPGDDHLAPDGRVMEVLSEHLCEGWLEGYLLTGRHGLFNCYE
                     AFIHIVDSMFNQHAKWLKVTRDIPWRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDH
                     VVNKKAAVVRVYLPPDANCLLSVMDHCLRSRDYVNVVVAGKQPSLDYLDAGQAAAHCA
                     RGLGTWEWASTGQGSEPDVVLACAGDVPTLEALAAADLLRRGLPELKVRLVNVVDLMR
                     LQPPEEHPHGLPGADFDALFPPGTPVIFAYHGYPWLIHRLTYRRAAQADLHVRGYKEE
                     GTTTTPFDMVMLNDLDRFHLVMDVIDRVPGLGASAVALRQEMADRRLAARAHTRAHGE
                     DDPAISHWVWPG"
     gene            1030..>1873
                     /locus_tag="VF954_07955"
     CDS             1030..>1873
                     /locus_tag="VF954_07955"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="catalyzes the formation of propanoyl phosphate from
                     propanoate and ATP; TdcD also has acetate kinase
                     activities and functions in anaerobic threonine
                     catabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate/acetate kinase"
                     /protein_id="HEX9531057.1"
                     /translation="MARVEVRVLVVNAGSSSLKLSVLDEGDRLVASADSAPVDRLGAG
                     ALRERVHQLGAFDAVGHRVVHGGTELVEPVLVDAPVRAALGRLADLAPLHQGPALEAI
                     DQLGPVIGDRPAVACFDTAFHASLPPAATTYAVPAEWRERYGVRRYGFHGLSHAYASR
                     RAAALLGPPPRSGRRLVTCHLGAGASLAAVLDGRCVDTTMGFTPLEGLVMATRSGTVD
                     PGLVLWLQTHVGLPAAEVADALEHRSGLAGLAGTGDMASVLRMSAAGDASARLALDVY
                     LHRLR"
BASE COUNT          234 a          704 c          672 g          263 t
ORIGIN      
        1 ggagacccgg cccggcgacg accacctggc gccggacggc cgggtgatgg aggtcctgtc
       61 cgagcacctg tgcgaagggt ggctggaggg ctacctgctc accggccgcc acgggctgtt
      121 caactgctac gaggcgttca tccacatcgt cgactcgatg ttcaaccagc acgccaagtg
      181 gctgaaggtc acccgtgaca tcccgtggcg gcggcccatc gcctcgctga actacctgct
      241 ctccagccac gtctggcgcc aggaccacaa cggcttctcg caccaggacc ccggcttcat
      301 cgaccacgtg gtcaacaaga aggccgccgt cgtgcgggtc tacctgcctc cggacgccaa
      361 ctgcctgctg tcggtgatgg accactgcct gcgcagccgc gactacgtca acgtggtggt
      421 ggccggcaag cagcccagcc tggactacct cgacgccggc caggccgccg cccactgcgc
      481 ccggggcctc gggacgtggg agtgggcgtc gaccggccag gggtccgagc ccgacgtcgt
      541 gctggcctgc gccggcgacg tccccacgct ggaggcgttg gcggccgccg acctgctgcg
      601 ccggggcctg cccgagctca aggtccggct cgtcaacgtc gtggacctga tgcggctcca
      661 gccgcccgag gagcacccgc acggcctgcc gggcgccgac ttcgacgccc tgttcccgcc
      721 cggtacgccg gtcatcttcg cctaccacgg ctacccgtgg ctcatccacc gcctcaccta
      781 ccggcgggcg gcccaggccg acctgcacgt gcgggggtac aaggaggagg ggacgacgac
      841 gacgcccttc gacatggtca tgctcaacga cctcgaccgc ttccatctgg tgatggacgt
      901 gatcgaccgg gtgcccgggc tcggcgcgtc ggcggtggcg ctgcgccagg agatggccga
      961 caggcgcctg gcggcccggg cccacacccg ggcccacggc gaggacgacc ccgccatcag
     1021 ccactgggtt tggcccgggt agaggtgcga gtcctggtcg tcaacgccgg atcgagcagc
     1081 ctgaagctgt ccgtcctaga cgagggggac aggctggtgg cgtcggccga ctcggcgccg
     1141 gtcgaccgcc tgggtgccgg ggcgttgcga gaaagggtgc accaactggg cgcgttcgac
     1201 gcggtcggtc accgggtggt gcacggcggg accgagctgg tcgagcctgt gctcgtcgac
     1261 gccccggtgc gcgccgccct cgggcggctg gccgatctcg cccccctgca ccagggcccg
     1321 gccctggagg cgatcgacca gctgggcccg gtgatcgggg atcggccggc agtggcctgc
     1381 ttcgacaccg ccttccatgc ctccctacct ccggccgcca ccacctacgc cgtgccggcg
     1441 gagtggcggg agcgctacgg cgtgcgccgg tacggcttcc acgggctgtc ccacgcgtac
     1501 gcgtcccggc gggcggccgc gctcctgggc cccccgcccc ggtccggccg gcgcctggtc
     1561 acgtgccacc tgggcgcggg ggcgtctctc gccgccgtgc tcgacggccg ctgcgtggac
     1621 acgaccatgg gcttcacgcc gctggagggc ctggtgatgg cgacccgctc gggcaccgtc
     1681 gaccccggcc tggtgctgtg gctccagacc cacgtgggcc tgccggcggc ggaggtggcc
     1741 gacgcgctgg agcaccgctc cggcctggcc ggcctggccg ggacgggcga catggcgtcg
     1801 gtcctgcgca tgtcggccgc cggcgacgcc tccgcccgcc tggccctgga cgtgtacctc
     1861 caccggctgc ggg
//
LOCUS       DASWGG010000434         4226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2482549, whole genome shotgun sequence.
ACCESSION   DASWGG010000434 DASWGG010000000
VERSION     DASWGG010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2482549"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VF954_07960"
     CDS             complement(<1..849)
                     /locus_tag="VF954_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531058.1"
                     /translation="MTGPNCTRALPAAASLAAALVLAAALGAAGPAGADTITSSPPAP
                     TAGQSYLLSSADGTVRAYGETAATGNAAINPAHPVVGLAASPSGKGWWMVGQDGGVFA
                     EGDAAFFGSTGAMRLNKPVVGMTASPSGKGYWFVASDGGIFSFGDAAFHGSTGAMRLN
                     KPVVGMATTPSGKGYWLVASDGGIFAFGDAAFYGSTGAMRLNQPIVGMAATPGGKGYR
                     FVAADGGIFSFGDAHFYGSSATSAPAAAAAAPVRGLAATATGLGYWVVDAAGGVRAFG
                     DAKPAGA"
     gene            complement(1010..2317)
                     /gene="serS"
                     /locus_tag="VF954_07965"
     CDS             complement(1010..2317)
                     /gene="serS"
                     /locus_tag="VF954_07965"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX9531059.1"
                     /translation="MIDIRRVRGDLAGVKAAMARRRDPSLLDQLDRLHRADAEWLAAV
                     AERDELRRRVNALSREVGAAYRAGDWARGDELKASSRTLGDREKGSAARVDELEATIR
                     SVLLLVPNLPSPDAPDGASEADNVVVRVEGNDPAAYGLHQRVPHWEVGPSLGILDNER
                     AVKISGAMFTMPRRGGATLSRALCQYALDRCAPAFEEVRPPSLVSSATLTATGQLPKF
                     ADDAYYIERDDLWAVPTAEVPLTSIYRDEILDEGDLPKRLMAYTPCYRREAGSAGRDT
                     RGMLRTHEFDKVEILALTTPAQAPAMHEELVARAERLIADLGLAYRIVDISAGDLGQS
                     HHRYFDIEVYAPGCDMWLEVSSISWFTDYQARRANIRYRPGSSGSSGSSEGPTELVHT
                     LNGSALAVPRVWAAVVETYRQPDGHVAVPSVLLPYFRGETLVG"
     gene            2377..3339
                     /locus_tag="VF954_07970"
     CDS             2377..3339
                     /locus_tag="VF954_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9531060.1"
                     /translation="MAAIEIADLRVQYGEVTAVDGLSFSVDAGRVLALLGPNGAGKTT
                     TVETLEGYRRPTSGTVRVLGLDPIGEHAALVPQIGVMLQRGGIYPGMPAGEAVRLFAA
                     YYNDPEDPVELMVRVGLHGVARTPWRRLSGGEQQRLSLALAMVGRPRVAFLDEPTAGM
                     DPAARQVLWGIVRELRDRGVCVLLTTHYLEEAERLADAVVIVDRGRKVAEGTPAALMA
                     SGGAREIRFGGPAGIDVDALGSALEAAVLEESPGEYLVRAEATPQRVAALTGWLAERD
                     VALADLRAGRETLEDVFLRLTGAPGGNEAVGPRRGRRTRRAGTA"
     gene            3336..4064
                     /locus_tag="VF954_07975"
     CDS             3336..4064
                     /locus_tag="VF954_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9531061.1"
                     /translation="MRALVAQARAEVAMTLRRGESILLAVGIPVLLLVFFSVVKVLPK
                     DPAARNEVSFLAPGILALAVMSTAMVSLGIATGFERSYGVLKRLGTTPLSRPALLAAK
                     TVAIVVVEAAQIVVLVPVGVALGWSPKGGGLVALAAVALGTIAFAGLGLLMAGTLRAE
                     VTLAAANGLYLALLLLGGMVVPLAKLPGALRAVARVLPSGALSDALHSALGTGAGVPG
                     RAWVVLVVWAVLAPAAAAATFRWE"
BASE COUNT          531 a         1487 c         1637 g          571 t
ORIGIN      
        1 cgcgccggcc ggcttggcgt caccgaacgc cctcaccccg ccggctgcgt ccaccaccca
       61 gtagccgagc ccggtggcgg tggcggccaa gccgcgcacc ggggccgcgg ccgcggcagc
      121 cggcgcgcta gtggccgacg acccgtagaa atgggcgtcg ccgaaggaga agatgccgcc
      181 gtcggccgcc acgaaccggt agcccttccc accgggcgtg gccgccatgc ccacgatggg
      241 ctggttgagc ctcatggcac cggtcgagcc gtagaacgcg gcgtcgccga acgcgaagat
      301 cccgccgtcc gaggcgacca gccagtagcc cttgccggaa ggggtcgtgg ccatgccgac
      361 caccggcttg ttgagcctca tggcaccggt cgagccgtgg aaggcggcgt cgccgaagga
      421 gaagatgccg ccgtcggagg ccacgaacca gtaccccttg cccgacgggc tggccgtcat
      481 gcccacgacc ggcttgttga gcctcatggc gccggtggag ccgaagaacg cggcgtcgcc
      541 ctcggcgaac accccgccgt cctggccgac catccaccag cccttcccgc tcggggacgc
      601 ggccagcccg accacgggat gggccgggtt gatcgccgcg ttgccggtcg cggccgtctc
      661 cccataggcc cgcacggtgc cgtcggccga cgaaagcagg tagctctggc cggcggtggg
      721 ggcgggcggg ctggaggtga tcgtgtcggc cccggcgggc ccggccgcgc ccagggcggc
      781 ggcgaggacc agggcggcgg cgagggaggc ggcggccgga agagcgcgcg tgcagttcgg
      841 cccggtcacc cggggattgt cggcttcggg ctcctgtgac tgaagtgcgc cgtgtggttg
      901 gtggggccgc gccggtggga cgaggacgtc gtcaaaacca ttcttcaagc aaaaaacacg
      961 ctgggacgac attaaagcat ggaagaatgc tcgaacaggc acaggagcgt cagcccacca
     1021 gggtctcgcc ccggaagtac ggcagcagca ccgaggggac ggccacgtgc ccatcgggct
     1081 ggcggtaggt ctcgaccacc gcggcccaga cccggggcac ggccagggcg gacccgttca
     1141 aggtgtggac cagctcggtc ggcccctccg aggacccgga cgagccggag gagcccgggc
     1201 ggtaccggat gttggcccgc cgcgcctggt aatcggtgaa ccacgagatc gacgacacct
     1261 ccagccacat gtcgcatccg ggcgcgtaca cctcgatgtc gaagtaccgg tggtgggact
     1321 ggcccaggtc acccgcgctt atgtcgacga tgcggtaggc cagccccagg tcggcgatga
     1381 gcctctcggc ccgagccacg agctcctcgt gcatggccgg ggcctgcgcg ggcgtggtga
     1441 gtgcgaggat ctccaccttg tcgaactcat gggtccgcag catcccccgc gtgtcccgcc
     1501 cggccgagcc ggcctcccgc cggtagcacg gcgtgtaggc catgagccgc ttaggcaggt
     1561 ccccctcgtc gaggatctcg tcgcggtaga tcgacgtgag cgggacctcg gcggtgggga
     1621 cggcccacag gtcgtcgcgc tcgatgtagt aggcgtcgtc ggcgaacttg ggcagctggc
     1681 ccgtcgccgt cagggtggcc gacgacacca gcgaaggggg ccgaacctcc tcgaaggccg
     1741 gcgcgcaccg gtccagggcg tactggcaca gggcccggct cagggtggcc ccgccccgcc
     1801 tcggcatggt gaacatggcc ccggagatct tcaccgcccg ctcgttgtcg aggatcccga
     1861 gcgacggtcc cacctcccag tggggcaccc gctggtgcag cccgtaggcc gcggggtcgt
     1921 tcccctccac gcgcaccacc acgttgtcgg cctcgctcgc cccgtccggg gcgtcgggcg
     1981 acgggaggtt cgggaccagc agcagcaccg accggatggt ggcctcgagc tcgtcgaccc
     2041 tcgcggccga ccctttctcc cggtcgccca gcgtccggct cgacgccttg agctcgtcgc
     2101 cgcgcgccca gtcccccgca cggtaggcgg ccccgacctc cctggacagg gcgttgaccc
     2161 ggcgtcgcag ctcgtcgcgc tcggccaccg cggccagcca ttcggcatcg gcgcggtgga
     2221 gccggtcgag ctggtcgagc aggctcgggt cgcggcgccg ggccatggcg gccttgacgc
     2281 cggcgaggtc gccccgcacc cttcggatgt cgatcatgag tggccgtcct cggcggcgaa
     2341 atccggtgcc aaagggggca cggtagcgtg ccgccggtgg ccgccatcga gatcgccgat
     2401 ctccgcgtcc agtatggcga ggtcaccgcg gtcgacggcc tgagcttctc ggtcgacgcc
     2461 gggcgggtgc tggcgctgct cggcccgaac ggcgccggca agaccaccac cgtcgagacg
     2521 ctcgagggct accgccgccc gacctcgggc acggtgcggg tgctcggcct cgacccgatc
     2581 ggcgagcacg ccgccctggt gccgcagatc ggcgtgatgc tccagcgggg tggcatctac
     2641 ccgggcatgc cggccgggga ggcggtgcgg ctcttcgccg cctactacaa cgaccccgag
     2701 gacccggtcg agctcatggt gcgggtgggc ctccacggcg tggcccgcac cccgtggcgg
     2761 cggctgtccg gaggcgagca gcagcggctg tccctggccc tggcgatggt ggggcggccc
     2821 cgggtggcct tcctcgacga gcccaccgcg gggatggacc ctgcggcccg ccaggtcctg
     2881 tggggcatcg tgcgggagct gcgagaccgg ggcgtgtgcg tgctgctgac gacccactac
     2941 ctggaggagg ccgagcggct ggccgacgcc gtggtcatcg tggaccgggg ccggaaggtg
     3001 gcggagggca cgccggcggc cctgatggcg tcgggcgggg cgcgggagat caggttcggg
     3061 ggaccggcgg gcatcgacgt ggacgcgctc ggttcggccc tggaggcggc ggttctggag
     3121 gaaagcccgg gcgagtacct ggtacgggcc gaggccacgc cgcagcgggt ggcggccctg
     3181 accggatggc tggccgagcg cgacgtggcc ctcgccgacc tgcgggcggg gcgggagacg
     3241 ctggaggacg tgttcctgcg actgacgggc gcaccgggtg ggaacgaggc ggtcgggccg
     3301 cggcgcggtc gccggacgcg gcgggcgggc acggcgtgag ggcgctcgtc gcccaggccc
     3361 gggccgaggt cgccatgacc ctgcgccgcg gggagtcgat cctgctggcc gtcgggatac
     3421 cggtgctgtt gctggtcttc ttctcggtgg tcaaggtgct gcccaaggac cccgcggccc
     3481 gcaacgaggt ttccttcctg gcgcccggga tcctcgccct ggcggtgatg tcgaccgcaa
     3541 tggtgagcct cggcatcgcc acgggcttcg agcgcagcta cggcgtgctc aagcggttgg
     3601 gcaccacacc gctgtcgcgg cccgctctgc tcgccgccaa gaccgtcgcc atcgtggtcg
     3661 tcgaagccgc ccagatcgtg gtgctggtgc ccgtcggcgt ggctctgggg tggagcccga
     3721 agggcggcgg gctggtggcg ctggcagctg tggcgctcgg cacgatcgcc ttcgccggtc
     3781 tcgggctgct gatggccggc acgctgcggg ccgaggtgac cctggccgcg gccaacggcc
     3841 tgtacctggc gctgctgctc ctcggcggca tggtcgtgcc cctggccaag ctgcccggcg
     3901 ccctgcgggc ggtggcgcgg gtgctgccgt cgggcgcgct gtccgacgcc ctgcactccg
     3961 ccctgggcac cggggcgggc gtgcccggcc gggcctgggt cgtgctggtg gtgtgggccg
     4021 tgctggcccc cgcggcggcg gcggccacct tccgctggga gtagccggcc ccaagtcggc
     4081 acggccgccg gcggggttgg ggcccccgcc gtgcggagga ggccggggtt ggggccccgg
     4141 ccgggcctaa tgggaacggc cgccggcggg gttggggccc ccgccgtgcg gaggaggccg
     4201 gggttggggc cccggccggg cctaat
//
LOCUS       DASWGG010000435         4808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2482859, whole genome shotgun sequence.
ACCESSION   DASWGG010000435 DASWGG010000000
VERSION     DASWGG010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2482859"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VF954_07980"
     CDS             complement(<1..483)
                     /locus_tag="VF954_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX9531062.1"
                     /translation="MSFPARRLRRLRRTPTLRRMVAETRVTVDDLIAPLFVREGIDEP
                     VPIASLPGVVQHTVDSLSKEARRLAGLGVPALVLFGVPATKDAQGSQAWARDGIVQVA
                     LRHLRDELGDDVVVIADLCLDEYTDHGHCGVLREDGSVDNDSTIDIYARVAVSQASAG
                     A"
     gene            complement(504..1967)
                     /gene="cobA"
                     /locus_tag="VF954_07985"
     CDS             complement(504..1967)
                     /gene="cobA"
                     /locus_tag="VF954_07985"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX9531063.1"
                     /translation="MTVYLVGAGPGDPGLMTVRGAELLAQADVVVYDRLADDELLGLA
                     PPLAECVDVGKRPGGPVDQSSINELLVERGRAGQTVVRLKGGDPFVLGRGGEEAEALA
                     AAGVAYEVVPGISSAIAVPAYAGIPVTHRGIATSFTVVTGHSRNQADDETDWDALARA
                     GGTIVVLMAVAHRREVAERLMAAGRPPGTPVAAIHWGTRPDQRTVRTTLAGLAGVDLE
                     PPATIVVGPVAALDLDWFESRPLFGRTVVITRPRAQAPALARRLKHLGAAVLDVPAVV
                     IRDPGDGGASLERAAREVAGYDWVVFTSANAVDRFLARLPDSRAFGPAQVAAIGPGTA
                     AALARWHVVADLQPAAYVAEELTAALLAAGAPGRVLVPRAAVARDVLPDGLRAAGWEV
                     DVAEAYRTAPAPIGPEQIAAARGADAIAFTSSSTVTGFLAAAGPEAVPPVVACIGPVT
                     AATARAAGVHVDVVAEEHTIDGLVGALVGALAPDARP"
     gene            complement(1964..2950)
                     /gene="hemC"
                     /locus_tag="VF954_07990"
     CDS             complement(1964..2950)
                     /gene="hemC"
                     /locus_tag="VF954_07990"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX9531064.1"
                     /translation="MALRVATRGSALARWQADAVARLLGEPVELVIVETTGDRRGDVP
                     ISAIGGQGVFVKEVQMAVLEGRADLAVHSAKDLPSVAAPGLVIAAIPERGDARDAMVG
                     ARLDELRPGAVVATGSVRRRAQLAGLRPDLTFTGLRGNIGTRLAKVPPGGAAVMAAAA
                     LQRLGCEDRMAQVLEPSVMVPQVGQGALAVECREDDASTRAVLARLDHEASRAAVEAE
                     RGFLAALGGGCDLPVGAYARVTGEGLEMEGLLASLDGRVVLRHRAGAPPGHPPDELGR
                     AVARHLVERAGGSSLLIEAGGDPRAIEAGGHPGGLEAGADPAGEAVTRRRPR"
     gene            complement(2955..4223)
                     /locus_tag="VF954_07995"
     CDS             complement(2955..4223)
                     /locus_tag="VF954_07995"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:NF000744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX9531065.1"
                     /translation="MSDVVVVGLNHRTVPLEVLERMTVSRSRLPKALHDLEARDHLAE
                     AVVLSTCMRTEIYVVAERFHGAVADVRDFLCEWGHTPPEALSDHLYSFHEDAAVAHLF
                     AVASGLDSAVLGETEVLGQVRDAWESAREHGAAGTVLSGLFRRAVEVGKRARSETAIS
                     RGTTSLSQAALALAADRLGGTLAGRSILVLGAGEMGEGMAVALAGSAGVAEVLVANRT
                     WSRAVALARRVGGRPVELGGLAAALQEVDVLLTSTGSPTVLLEADDLVEVLPARAGRT
                     LLVVDAAVPRDVDPAVGRLHGVTLLDMDDLRAFAERAMAGRRREVAAVREIVAEEVER
                     YQGEASARSVVPLVTALRERAETLRLAELARYRHRLEGLEEREREALEAVTRGLINKL
                     LHEPTVRLKDAAGTARGERLADALRTLFDL"
     gene            complement(4202..>4808)
                     /locus_tag="VF954_08000"
     CDS             complement(4202..>4808)
                     /locus_tag="VF954_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEX9531066.1"
                     /translation="GRRVLVVGGGRVAARKVAGLLPCGAIVTVVAPEIGPELAELDQP
                     GQRDRFEVARRPYGRGEAASYDLVFTATSDAEVNQAVYDDAVAAGVWVNSADDPDRCS
                     FVLPSVVRRGPITVTVSTGGHSPAMSVFLRRWVSDELGPEYEVLVTLLSEQRAALQAA
                     GRATEGLDWQSALDSDMLDLVRAGQLDRVRERLKACLTSSS"
BASE COUNT          624 a         1847 c         1802 g          535 t
ORIGIN      
        1 ggcgccggcc gacgcctgcg acacggcgac gcgcgcgtag atgtcgatgg tgctgtcgtt
       61 gtcgacactg ccgtcctcgc ggaggacgcc gcagtggccg tggtcggtgt actcgtcgag
      121 gcagaggtcg gcgatcacca ccacgtcgtc gccgagctcg tcgcggaggt ggcggagggc
      181 gacctgcacg atgccgtcgc gggcccacgc ctgtgagccc tgggcgtctt tggtcgcggg
      241 cacgccgaac aggacgaggg cggggactcc gaggccggcg aggcggcggg cctccttgct
      301 cagcgagtcg acggtgtgct ggaccacgcc gggcagcgac gcgatcggga ccggctcgtc
      361 gatgccctcg cggacgaaaa gcggggcgat gaggtcgtcg acggtcactc gcgtctcggc
      421 gaccatgcgc cgcagggtgg gcgtgcggcg gaggcgacgg agccggcggg cgggaaagct
      481 cacctctcca gcctacgacc ggctcacggc cgggcgtcgg gcgcgagggc gccgacgagg
      541 gcgccgacca gcccgtcgat ggtgtgctct tcggcgacca cgtcgacatg gacgccggcg
      601 gcgcgcgccg tggccgcggt gaccggcccg atgcaggcca ccacgggcgg caccgcctcg
      661 gggccggccg cggcgaggaa gcccgtcacc gtggacgacg acgtgaaggc gatggcgtcg
      721 gcgccgcggg cggcggcgat ctgctccggg ccgatcggcg ccggtgccgt acggtaggcc
      781 tcggccacgt ccacctccca gccggcggcc cgcagcccgt cgggcagcac gtcccgggcg
      841 accgcggcgc gcggcacgag gacccgcccg ggcgctcccg cagccaggag cgcagcggtc
      901 agctcctcgg ccacgtacgc cgccggctgg agatcggcca cgacgtgcca gcgggcgagg
      961 gccgccgccg tgcccgggcc gatggcggcg acctgcgccg ggccgaaggc gcggctgtcc
     1021 ggcaaccggg ccaggaaccg gtccaccgcg ttggccgacg tgaagaccac ccagtcgtag
     1081 cccgccacct cccgcgccgc cctctcgagc gaggcgccgc cgtcgccggg gtcccggatc
     1141 accacggcgg gaacgtcgag gaccgccgcg ccgaggtgct tcagccggcg ggcgagtgcg
     1201 ggggcctggg ccctcggccg ggtgatcacg acggtccggc cgaagagcgg gcggctctcg
     1261 aaccagtcga gatcgagggc ggcaaccggg cccacgacga tggtggccgg gggctcgagg
     1321 tcgacgccgg cgaggccggc caacgtcgtg cgcacggtgc gctggtcggg gcgcgtgccc
     1381 caatggatgg cggccaccgg cgtgccgggt ggccggccgg cggccatcag ccgctccgcg
     1441 acctcccggc ggtgggcgac ggccatgagc acgacgatgg tcccgccggc gcgggccagg
     1501 gcgtcccagt cggtctcgtc gtcggcctgg ttgcgcgagt gccccgtcac gaccgtgaac
     1561 gacgtggcga tgccccggtg ggtcacgggt atgcccgcat aggccggcac ggcgatggcg
     1621 gaggagatgc ccgggactac ctcgtacgcc acgccggccg ccgccagggc ctcggcctcc
     1681 tcgcccccgc gccccagcac gaaggggtcg ccccccttga ggcggaccac cgtctggccg
     1741 gcccggcccc gctccaccag cagctcgttg atggacgact ggtcgacggg gcccccgggc
     1801 cgcttgccga cgtcgacgca ctccgccagg gggggcgcca ggccgagcag ctcgtcgtcg
     1861 gccagccggt cgtagacgac cacgtcggcc tgggccagca gctcggctcc ccggacggtc
     1921 atgagaccag gatcgccggg gccggccccg accaggtaga cggtcaccgg ggccggcgcc
     1981 gggtgacggc ctcgccggcc gggtcggctc ccgcctcgag gccccccggg tggcctccgg
     2041 cttcgatggc cctcgggtcg cctccggctt cgatgaggag cgaggagcca cccgccctct
     2101 cgaccagatg ccgggcgacc gcccgcccga gctcgtccgg cgggtgtccc ggaggggcgc
     2161 cggcgcggtg gcgcagcacg acccgcccgt cgagggaggc gagcaggccc tccatctcga
     2221 ggccctcgcc ggtcacgcgc gcgtaggcgc ccacgggcag gtcgcagccg ccgccgaggg
     2281 cggcgaggaa gccccgctcg gcctcgacgg ccgcccggct ggcctcgtgg tcgaggcggg
     2341 caaggacggc ccgggtcgag gcgtcgtcct cgcggcactc caccgcgagc gcgccctgcc
     2401 ccacctgggg gaccatgacc gagggctcga ggacctgggc catgcggtcc tcgcaaccga
     2461 ggcgctggag ggcggccgcg gccatgaccg cggcgccgcc gggcggcacc ttggcgaggc
     2521 gggtgccgat gttgccgcgc aggccggtga aggtgagatc gggccgcagc cctgccagct
     2581 gggcccggcg ccggaccgac ccggtggcga ccaccgcgcc ggggcgcagc tcgtccagcc
     2641 gggcgccgac catggcgtcg cgggcgtcgc cgcgctcggg gatggccgcg atcaccaggc
     2701 cgggcgccgc caccgacggc aggtccttgg ccgaatgcac ggccaggtcg gcgcggccct
     2761 ctagcaccgc catctggacc tctttcacga acacgccctg cccgccgatg gcagagatcg
     2821 gcacgtcgcc ccggcggtcg ccggtcgtct cgacgatcac cagctccacc ggctcaccca
     2881 acaagcgggc cactgcgtcg gcctgccagc gggccagggc gctgccgcgg gtggcgaccc
     2941 ggagagccac ccgtctagag atcgaagagg gtgcgcaggg cgtcggccag gcgctcgccc
     3001 cgggcggtgc cggcggcgtc cttcaaccgg accgtcggct cgtggagcag cttgttgatc
     3061 aggccccggg tcaccgcctc gagcgcctcg cgctcgcgtt cctccaggcc ctccaggcgg
     3121 tgacggtagc gggccagctc ggcgaggcga agcgtctccg cccgctcgcg cagggcggtg
     3181 acgagcggga ccacggagcg cgccgacgcc tcaccctggt accgctcgac ctcctcggcg
     3241 acgatctcgc gcacggcggc gacctcccgc cgccggccgg ccatggcccg ctcggcgaag
     3301 gcccgcaggt cgtccatgtc cagcagcgtc accccgtgca gccgtccgac ggcggggtcc
     3361 acgtcccgcg gcacggcggc gtccaccacg agcagcgtcc ggccggcgcg cgccggcagg
     3421 acctccacca gatcgtccgc ctcgagcagg accgtcggcg acccggtgga cgtgagaagc
     3481 acgtccacct cctggagggc cgcggccagc cctccgagct cgaccggccg cccacccacc
     3541 cggcgggcca gcgccacggc gcggctccac gtccggttgg cgacgaggac ctcggccacc
     3601 ccggccgagc cggcgagggc cactgccatg ccctccccca tctccccggc tcccagcacc
     3661 aggatcgacc ggcccgccag cgtgcccccg aggcggtcgg cggccagggc gagcgcggcc
     3721 tgcgagagcg acgtcgtgcc ccgggagatc gccgtctcgg agcgggcccg cttgcccacc
     3781 tcgacggccc ggcggaacag cccgctcagg accgtgcccg cggccccgtg ctcccgggcg
     3841 ctctcccacg catcgcgcac ctggccgagg acctcggtct cgccgagcac cgccgagtcc
     3901 aacccggagg ccacggcaaa caggtgcgcg acggcggcgt cctcgtggaa gctgtagagg
     3961 tggtctgaca gggcctcggg gggggtgtgg ccccactcgc acaggaagtc gcgcacgtcg
     4021 gcgaccgccc cgtggaagcg ctcggccacc acgtagatct ccgtgcgcat gcacgtggag
     4081 aggacgaccg cctcggcgag gtggtcgcgc gcctcgagat cgtgcagcgc cttcggcagg
     4141 cgcgagcggc tcaccgtcat ccgctcgaga acctccagcg ggacggtgcg gtggttgagg
     4201 cctacgacga cgacgtcaga cacgctttca ggcgctccct cacccggtcc agttgaccgg
     4261 ctcgtaccag atcgagcatg tccgagtcga gggcgctctg ccaatcgagg ccctcggtgg
     4321 cgcgaccggc ggcctggaga gcggcccgct gctcggagag cagggtgacc agcacctcgt
     4381 actccgggcc cagctcgtcg gagacccagc ggcggaggaa cacagacatc gccgggctgt
     4441 gccccccggt cgagacggtc accgtgatcg ggccgcgccg taccaccgac gggagcacga
     4501 agctgcaccg gtcggggtcg tccgcgctgt tgacccacac gccggccgcg acggcgtcgt
     4561 cgtagaccgc ctggttgacc tccgcgtcgg acgtcgccgt gaacaccagg tcatagcttg
     4621 ccgcctcgcc gcgcccatag gggcgtcgcg ccacctcgaa ccggtcgcgc tggccgggct
     4681 ggtcgagctc cgccagctcc ggaccgatct ccggcgcgac caccgtcacg atggcgccac
     4741 agggcagcag cccggccacc ttgcgggccg cgacccgccc cccgcccacc accaggacgc
     4801 ggcgcccg
//
LOCUS       DASWGG010000436         3734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2250749, whole genome shotgun sequence.
ACCESSION   DASWGG010000436 DASWGG010000000
VERSION     DASWGG010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2250749"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VF954_08005"
     CDS             complement(<1..739)
                     /locus_tag="VF954_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531067.1"
                     /translation="MLTRAGTRVAGGLAVALLATMLAAPVGAAVADNYWKVDAWGHSV
                     EPYAQIPEELDAGSPGAWVQLAGKLPVQCEANGTLIDTGGSGETVDQLGYELSDHNYH
                     NPGKAAAANPENRNPVKADVTTFPNGPESKAECASPTSGASSATWGRYLSKDFSFESA
                     SSDSTDTKSGEQGSIVTETTNKVQGVKIGPLSIGTVLSWLKVEFRQSAEPVISYRIEL
                     TGIQDGQNQSGAGYTGVALAGQGLVGCD"
     gene            complement(756..1439)
                     /locus_tag="VF954_08010"
     CDS             complement(756..1439)
                     /locus_tag="VF954_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9531068.1"
                     /translation="MLELRGIRAGYGRLPVLFDVDLTVAAGEWLAVVGANGAGRSTLL
                     KTILGVTNVSAGDVMFGGQRITHLATYDRIARGIAMVPEGRRIFPNLSVKENLRTGAN
                     TISKRDVPGQLERVYEVFPILDSRSGQRAGSLSGGEQQMLAVGRALMSRPQLLLVDEL
                     SLGLAPLVVATLTEALQTLCAGGLAVIVVDEVHVHRTTASASQRLVLEKGRFITNSAA
                     EAERLATVL"
     gene            complement(1432..3297)
                     /locus_tag="VF954_08015"
     CDS             complement(1432..3297)
                     /locus_tag="VF954_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9531069.1"
                     /translation="MKRRAVIGAVLAAVAAVWLFTAPQYWLFTATTGLTLAISTLGLL
                     VLVGWAREVSLAQAGLTATAIYLCGFAMRAGAGWNWPYLAAAALGIGVVVLLSLLVSL
                     STAKLSGIYIIILTLALQVTIEKTFFSNIKLVETDPEHTIHRPALLGVHLTSDRAYFL
                     FALAVLVLCMLFLARLRASRFGRGLMLAGTDRQAAAAVGVSPWRAKIFAFALAGFFAG
                     VAGVVTAPLYPTPPSYISYLSINSLVYLAIPVLAGFRSLTAVAVVAMVFSVAPQALEH
                     LHISPLMLGAIGLLCGTLTGPTGVSGLILAWIHSRRRTRGADEAGVDVRDDPTAWAED
                     ARQERHARALAVLEQYLPERSDVGDVLVANDVSIAFGGLQALSDVSLTVPTRKLVGLI
                     GPNGAGKSTLFDILNGLRQPDSGSVLLFGQDVSQAPPWDRAALGLSRTFQSSRVNLDL
                     TVGENLMAGAYLMIPGSVVEAVAGMPRAQTGERRAQEAGRAVAELLDVAADWDEFVRN
                     LDFGAQRRVEIGRSLLSGPRLLLLDEPAAGLDASEATALFALIRQLEQDLGLTVLLVE
                     HYVKAVLENCDLIYVLARGRIIAAGSAEEITADPEVRAVYLGEDYQQREEVRADA"
     gene            complement(3309..>3734)
                     /locus_tag="VF954_08020"
     CDS             complement(3309..>3734)
                     /locus_tag="VF954_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531070.1"
                     /translation="AFVLMRTQTGVNLRAVADDVEASRFVGVPLTKVGAGVYAFSAGL
                     SAFAGALVTATVGMLVPELLTVFLRALTVCVLGGLASFSLALLGCVLLGVGEAFLTAG
                     VFGQSSIGRQEVILMTALFGLVLLINRLRPIKVLEVETL"
BASE COUNT          573 a         1320 c         1329 g          512 t
ORIGIN      
        1 ggtcgcaacc cacgaggccc tggccggcca gggcgacgcc ggtgtacccc gccccgctct
       61 ggttctgccc gtcctggatc cccgtgagct cgatccggta ggagatcacc ggctcggctg
      121 attgccggaa ctccaccttg agccagctca acaccgtgcc gatcgacagc ggcccgatct
      181 tgaccccctg gaccttgttg gtggtctcgg tcacgatgga gccctgctcg cccgacttcg
      241 tgtcggtgct gtcgctggag gccgactcga aactgaagtc cttgctcagg taccgccccc
      301 acgtcgccga cgatgcccca gaggtgggcg aggcgcactc ggccttcgac tccggcccgt
      361 tggggaaggt ggtcacgtcg gccttgacgg ggttgcggtt ctcggggttg gccgccgccg
      421 ccttccccgg gttgtggtag ttgtgatccg acagctcgta gccgagctgg tcgacggtct
      481 cgccgctgcc gccggtgtcg atcagcgtcc cgttggcctc gcactgcacc gggagcttgc
      541 cggcgagctg gacccaggcc cccggcgatc cggcgtcgag ttcctcgggg atctgggcgt
      601 agggctcgac ggaatggccc cacgcgtcga ccttccagta gttgtcagcc acggcggctc
      661 ccaccggagc cgccagcatc gtcgccagca gcgccacggc cagcccgccc gccacccgag
      721 ttcccgctct cgtcagcatt gatccttcct tcctcctaga ggacggtcgc caaccgttct
      781 gcttccgcgg ccgagttcgt gatgaaccgg cccttttcca ggacgagtcg ctggctcgcc
      841 gacgccgtgg tgcgatgcac gtggacctcg tcgaccacga tcacggccag gcctccggca
      901 cacagcgtct gcagcgcttc ggtcagggtc gccacgacca gcggggcgag gcccagtgag
      961 agctcgtcga ccagcaggag ctgcgggcgt gacatcaggg cccggcccac ggccagcatc
     1021 tgctgctctc ccccggagag cgacccggcc cgctgcccgg agcggctgtc gaggatgggg
     1081 aacacctcgt agacccgttc cagctggccc ggcacgtccc gcttggagat cgtgttggcc
     1141 cccgtccgca ggttctcctt gacgctcagg ttcgggaaga tccgccggcc ctcggggacc
     1201 atggcgatgc cccgggcgat ccggtcgtag gtcgccagat gggtgatgcg ctgcccgccg
     1261 aacatgacgt cgccggcgct gacgttggtc acgccgagga tcgtcttcag gagggtggac
     1321 cggccggcgc cgttcgcccc caccaccgcc agccattcgc cggccgccac ggtgaggtcg
     1381 acgtcgaaga gcaccggaag ccggccgtat ccggcccgga tgccgcgcag ctcaagcatc
     1441 ggcccgcacc tcctccctct gctggtagtc ctcgccgagg tagacggccc gcacctccgg
     1501 gtcggcggtg atctcctccg cgctcccggc ggcgatgatc cgtccccggg cgaggacgta
     1561 gatcaggtca cagttctcga gcaccgcctt gacgtagtgc tcgacgagca gcacggtcag
     1621 ccccaggtcc tgctccagct ggcggatgag ggcgaagagc gccgtcgcct cgctggcgtc
     1681 gagcccggcg gcgggttcgt cgagcaggag caggcggggg ccggagagca ggctccggcc
     1741 gatctcgacc cggcgctgag ccccgaagtc gaggttgcgg acgaactcgt cccagtcggc
     1801 cgccacgtcg agcagctcgg ccaccgcccg gcccgcctcc tgggcccgcc gttcgccggt
     1861 ctgggcccgg ggcatcccgg ccacggcctc gaccacgctg ccggggatca tgaggtaggc
     1921 cccggccatc aggttctcgc ccacggtgag gtcgaggttc acccggctgg actggaaggt
     1981 gcgggacaga ccgagcgcgg cgcggtccca cggcggtgcc tggctcacgt cctggccgaa
     2041 gaggaggacc gacccgctgt cgggctggcg gagcccgttc aggatgtcga agagcgtgga
     2101 cttcccggcc ccgttcggcc cgatcagacc gaccagcttg cgggtcggga cggtgagcga
     2161 cacgtcggag agcgcctgca ggccgccgaa ggcgatcgag acgtcattgg ccaccagcac
     2221 gtccccgacg tcgctccgtt ccggcaggta ctgctcgagg accgccaggg cccgggcgtg
     2281 gcgctcctgg cgggcgtcct cggcccaggc ggtcgggtcg tcgcgcacgt cgaccccggc
     2341 ctcgtccgcg ccccgggtgc gccggcggct gtggatccaa gcgaggatga gcccgctcac
     2401 cccggtcggc ccggtcagcg tcccgcacag gagcccgatg gccccgagca tcagcgggga
     2461 gatgtgcagg tgctccaggg cctgcggggc gacggagaag accatcgcca ccaccgccac
     2521 cgcggtcagg gaccggaacc cggccagcac cgggatggcc aggtagacga gcgagttgat
     2581 cgacaggtag ctgatgtagc tcggcggcgt cgggtacagc ggggcggtga cgacgccggc
     2641 caccccggcg aagaagccgg ccagggcgaa ggcgaagatc ttcgcccgcc acggtgacac
     2701 accgaccgcc gccgcggcct ggcggtcggt cccggccagc atgagccccc ggccgaagcg
     2761 ggaggcccgg agccgggcga ggaacagcat gcacaggacc agcaccgcca gcgcgaacag
     2821 gaagtaggcc cggtcggagg tgaggtggac cccgagcagc gccggccggt ggatcgtgtg
     2881 ctccgggtcg gtctcgacca gcttgatgtt ggagaagaac gtcttctcga tcgtgacctg
     2941 cagggccagg gtcaggatga tgatgtagat ccccgacagc ttggcggtgg agagggagac
     3001 gaggagcgac agcaggacga ccacgccgat gcccagcgcc gccgccgcca ggtacggcca
     3061 gttccagccc gccccggccc gcatggcgaa cccacagagg tagatggccg tggcggtgag
     3121 gccggcctgg gccaggctga cctcccgggc ccagcccacc agcaccagca accccagcgt
     3181 ggagatggcc agggtgagcc cggtggtggc ggtgaagagc cagtactgcg gagcggtgaa
     3241 gagccagacg gcggcgaccg cggcgaggac ggccccgatg acggcccgtc gtttcatggt
     3301 tgcggccgtc acagcgtctc gacctccagc accttgatcg gccgcagccg gttgatgagc
     3361 aggaccagcc cgaacagggc cgtcatgagg atgacttcct ggcggccgat cgacgactgg
     3421 ccgaacaccc cggcggtgag gaacgcctcg cccacgccga gcaggacgca gcccagcagc
     3481 gccaggctga agctggccag ccccccgagg acgcagacgg tcagcgcccg gaggaagacg
     3541 gtgaggagct cggggaccag catgccgacg gtggccgtga ccagcgcccc ggcgaaggcc
     3601 gagaggccgg ctgagaaggc gtagacgccg gcgccgacct tggtgagggg cacgccgacg
     3661 aaacgggacg cctccacgtc gtcggccacg gcccggaggt tgaccccggt ctgggtccgc
     3721 atcagcacga aggc
//
LOCUS       DASWGG010000437         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2483208, whole genome shotgun sequence.
ACCESSION   DASWGG010000437 DASWGG010000000
VERSION     DASWGG010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2483208"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VF954_08025"
     CDS             complement(<1..288)
                     /locus_tag="VF954_08025"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX9531071.1"
                     /translation="MSRVRAGESFLVVGAPSRRGSGLAAERGDAFVELVVVAPLLLLM
                     LGVAVVMGQLLDAKASAADVAREAARIAVEQPGARAGGDAATRAARDRAAAL"
     gene            complement(285..689)
                     /locus_tag="VF954_08030"
     CDS             complement(285..689)
                     /locus_tag="VF954_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531072.1"
                     /translation="MSGRLRGLGRAEEGLAALEIVIAGGVLVVSVLAVVQFALWFHAR
                     RALHAAAVIGAHSVAVDRKSDLQAEAAATDFLRASTGGLLLGSSASVTTGPADVDVVM
                     QASLGSFVPGLRAIRLRTEVIQHRELLGSARP"
     gene            complement(755..1252)
                     /locus_tag="VF954_08035"
     CDS             complement(755..1252)
                     /locus_tag="VF954_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531073.1"
                     /translation="MALLQKIPMPTASIAMFPSQTYGGICGIVSSFWATGYDGKPIQW
                     SGPTAGGGVVDVLATPQGFDWDFGDSFRVESSGPGVAWPELNGAVTHKYRVKSKAAGF
                     PTGAVNSGQGGYPVSLTVVFDVVWRLNGGAWRAGLAPIQRAITANYPVYEVRGEEVPN
                     FAAAG"
BASE COUNT          220 a          586 c          526 g          204 t
ORIGIN      
        1 gagggcggcg gccctgtccc gcgccgccct ggtcgcagcg tcgccgccag cccgtgcgcc
       61 tggctgctcg acggcgatgc gggcggcctc cctggcgacg tcggccgccg acgccttggc
      121 gtcgaggagc tgccccatca ccacggccac gccgagcatc agcagcagca gcggtgcgac
      181 gacgaccagc tcgacgaagg cgtccccgcg ctcggcggcc aggccgctcc cccgccggct
      241 gggggcaccg acgacgagga atgactcccc tgcccgcacg cggctcacgg cctcgccgac
      301 cccagcagct cgcggtgctg gatgacctcg gtgcgcagcc ggatcgcccg caggcccggg
      361 acgaacgagc ccaggctggc ctgcatgacg acgtcgacgt cggcggggcc ggtcgtgacc
      421 gacgccgagg accccaggag caggccgccg gtgctcgccc gcaggaagtc ggtcgccgcc
      481 gcctcggcct ggaggtcgct ctttcggtcg acggccacgc tgtgggcccc gatcaccgcc
      541 gcggcgtgca gcgcccggcg ggcgtggaac cacagggcga actgcacgac ggccagcacc
      601 gagacgacca gcaccccgcc ggcgatgacg atctcgagcg cggcgaggcc ctcctcggcc
      661 cggccaagcc cacgcagtcg acctgacatc cttcctcccc tgctcgccac ttcggtcccc
      721 ggtggtcgcc cacgactggg ggctccatcc ggcgtcaccc ggcggcggcg aagttgggca
      781 cctcctcgcc gcggacctcg tagacggggt agttcgccgt gatggcgcgc tggatcgggg
      841 caagcccggc ccgccaggct ccgccgttca gccgccacac gacgtcgaac acgacggtga
      901 gcgagacggg atagccgccc tgcccgctgt tgacggcccc ggtggggaat cccgcggcct
      961 tgctcttcac ccggtacttg tgggtcaccg cgccgttcag ctcaggccag gcgacgccgg
     1021 gtcccgagct ctcgacgcgg aaactgtcgc cgaagtccca gtcgaagccc tggggggtgg
     1081 cgagcacgtc caccactccc ccaccggcgg tcggacccga ccactggatc ggcttgccgt
     1141 cgtagccggt cgcccagaac gagctcacga tcccgcagat cccgccgtag gtctggctcg
     1201 ggaacatggc gatcgaggcc gtcggcatgg ggatcttctg cagcaacgcc accgcggttc
     1261 cctggggcgt gacaggcagc gggcaacctc ccggaggaca gggtgcctgt ggtgtcaccc
     1321 acacaactcc ggtcgggctc ccgtcgcaca tctgaatcat gggcactgtc ggaggcccgg
     1381 cctccggctg ctgtccttgc tgttgcgatc cgctgatcag accaccgatc acatcgccga
     1441 actgttgctg agcgggtgcg gcgtcccata gaacgcacgc tggaccggtc ggccggatag
     1501 cgacggaggc aggccccagc gtgtcgcact ccgcct
//
LOCUS       DASWGG010000438         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2253101, whole genome shotgun sequence.
ACCESSION   DASWGG010000438 DASWGG010000000
VERSION     DASWGG010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2253101"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..380)
                     /locus_tag="VF954_08040"
     CDS             complement(45..380)
                     /locus_tag="VF954_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531074.1"
                     /translation="MRVELANAKDQARRLAQEVTVLRERLARQLGSDADLARGRALSP
                     LLDQLEQRAADLEAANNRQRQRILQLEAEARELTDTLEAARAMNRELMSELNRGTDRG
                     GSAAPLRRH"
     gene            complement(1116..>2049)
                     /locus_tag="VF954_08045"
     CDS             complement(1116..>2049)
                     /locus_tag="VF954_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9531075.1"
                     /translation="WLERTPSRKHRPGWDRRRATDGRVVVLGAARSPATVDGILAAVV
                     EFVRFGASRGWCVPSVAERLSCKTELRFAPPGWDRGERTDRAVVERRVVRRRRVERPP
                     ATLTRDQVGELVDACSNIRDRFVVEALYATGLRLSELCGLHLGDLHLVPSAAHLGCKV
                     AGAHLHVLRREDNENGALAKSIWPRVVPVTKHLVWCHDAYRFERDAVPAAAESDYLLV
                     NLWRAPVGRAMSPDAVEQLFARLSRKVRFHARPHMLRHSFASEVALATKDPALVKELL
                     GHASVSSTDVYLHARWDDMRAAVDNHARLRGAAR"
BASE COUNT          310 a          728 c          741 g          270 t
ORIGIN      
        1 ggcggagtgt gaccgacgtc gttcacacac cgagttggtc ggcatcagtg tcggcgcagg
       61 ggcgcggcgg atccgccccg atcggttccc cggttgagct cgctcatgag ctcccggttc
      121 atcgcccggg cagcctcgag cgtgtcggtg agctcacgag cctcggcttc gagttggagg
      181 atgcgttggc gttgccggtt gttggcggct tctaggtcgg ccgcccgctg ctcgagctga
      241 tcgaggagcg ggctgagagc ccgcccccgg gcgaggtcgg catcggagcc gagttggcgg
      301 gccagccgtt cgcgcaggac cgtgacctct tgggccagcc ggcgggcctg gtccttggcg
      361 ttggcaagct cgacccgcat ggcctgttcg gtgacgaggg aacgggtggg gagtcgccag
      421 gcccgctccc gaccggcttg gcgctggcgg tcgcgggcgg cggcgatgcg ggcggtgagg
      481 tcggggtcgg cgtagagcag cgagacggag acctcggcgc ggcgggcgac ggcgggaaag
      541 gcgccagatc tggaagtggg tgtcgcacag cccggcctcg ccgtgggcgg cgcccaggta
      601 gcagcatgct gccacgcacg ggccgaggtc cggcaaggcc cgggactggg cgaggaagtc
      661 ctcgaaggtg ccgctccgtg cccggcgccg gtcccacgcg ttggcatgca tcgtgcagag
      721 acgccgcggc ccttgcgctg ggcggggaca gcctcgcccg tcgacgccac tcaccgcgca
      781 gcgctgatcg cagcagcggt gccgctcgag gacggcgggc tcaccgctgg ccagccacgc
      841 ttcgtgatcc gaaatggtcg aggtggcgaa ctggcggcgg tggcgggcac acagagggct
      901 ggacccgtga cggtcgctcg ggcagtcggc cacgacgcag cgcaggaccc tggtgaggcg
      961 ccccccggga cggggcagca ccaggagccg ctccgagtcc cactcgccct ccagccagtc
     1021 ctcgcccatc agcgaagaga gccgctcgac ccgccagtcc actcgttccg ggcgaagcgg
     1081 ctccggggcg gccatggcga cagcaggacc ccgactcatc gggcggcccc tctgaggcgg
     1141 gcatgattgt cgacggcggc gcgcatgtcg tcccaacggg cgtgcaggta cacgtcggta
     1201 gaactgacgg aggcgtgccc cagcaactcc ttcaccaggg ccgggtcctt ggtagccagc
     1261 gcgacctcgg aagcgaagct gtgacgcagc atgtgtggcc gggcgtgaaa gcgcaccttt
     1321 ctcgacaggc gggcgaagag ctgctccacc gcgtcgggag acatggcgcg cccaaccggg
     1381 gcacgccaca agttgaccag caggtaatcg ctctcggcgg cggccggaac cgcatcccgc
     1441 tcgaagcggt acgcgtcgtg gcaccacacc aggtgctttg tgaccggcac cacccgcggc
     1501 cagatcgact tcgccagcgc cccgttctcg ttgtcctccc ggcgcagcac gtgcaggtgc
     1561 gccccggcca ccttgcaacc caggtgtgcc gcggagggaa ccaggtgaag atcaccaagg
     1621 tgcagtccgc acagctcgga gagccgcagg cccgtcgcgt acaacgcctc gacgacgaag
     1681 cggtcccgaa tgtttgagca cgcgtcgacc agctcgccga cctggtcgcg ggtgagcgtg
     1741 gcaggcgggc gctcgacccg ccgacgccgg acgacacgcc gctcgaccac ggcacgatcg
     1801 gtccgttccc cccggtccca gcccggcgga gcgaaccgca gctcggtctt gcagctcagc
     1861 cgctcggcca ccgagggaac acaccacccc cgggaggcac cgaagcgcac gaactccacc
     1921 accgccgcca ggatcccatc gaccgtggcc ggcgaacggg ccgcccccaa cacgaccacc
     1981 cggccgtccg ttgcccgccg gcggtcccag cccgggcggt gcttcctcga cggtgtccgc
     2041 tccaaccac
//
LOCUS       DASWGG010000439         2829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2490134, whole genome shotgun sequence.
ACCESSION   DASWGG010000439 DASWGG010000000
VERSION     DASWGG010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490134"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1038
                     /locus_tag="VF954_08050"
     CDS             82..1038
                     /locus_tag="VF954_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9531076.1"
                     /translation="MSPDPAVETWGLSRRFGPVTALDGLGISVAEGTVYGFLGPNGAG
                     KTTTIRLLLGLLEASGGWARVLGLDPRTAGAELRRQCGVLLEHPGLYERLSAEDNLDY
                     FGRLWRIPAGERQLRIVEVLDHVGLADRRTELVGTWSRGMKQKVAIARALLHRPRLVF
                     LDEPTAGLDPVAAAALRNDLLSLVGQEGVTVFLTTHNLDEAQRVCDRVGVVRSGRLVA
                     QGTPDELRSTAGRPTVTVRGSSVDLGLAAVVARPEVAGARIDGDALVVELRSPGPVAP
                     IVRLLVEAGVGIEEVHQGAASLEEAFLALLADPASVAPEPAT"
     gene            1035..1751
                     /locus_tag="VF954_08055"
     CDS             1035..1751
                     /locus_tag="VF954_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9531077.1"
                     /translation="MIADVIADAVTIAGKELKEILSRGGTARGRRGMIGTVVRPALVG
                     LLFGLNIGRGRSGAALAVLPVAFFAMATASALVADTIAGERERHTLETLLVSPASDES
                     ILAGKLGAVVLYAWAVALIQLLAVAVTGIALSPAHRVSAGLIAVVALFALLEATLSAA
                     LGVQFSVRAATVRAAARSQAQVGIVLSLLAGGANFAVADPRLGGPVVAGAAFVVLVAA
                     DAVLLGLARARFRRGRLLVA"
     gene            1895..2779
                     /locus_tag="VF954_08060"
     CDS             1895..2779
                     /locus_tag="VF954_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531078.1"
                     /translation="MGRLRSAILVATAVGAVLAVSAGLAGCGGGGGVPPLSIVLAAST
                     NTVAARTASVHVSTIITPNAAAPANPVLNGQPLVGDGKEDFGAHVATVTVQVPRAGSI
                     EVVYSGHVIYERIPQLAAQTGKAWVKIDLDNLGKRAGVSGLSSLAQGSGGDPSEGLSY
                     LRGAVGAVVTAGHEKVRGVQTTHYKATTDVNRAAAQLPVAQQATTKQISDQFGITTIP
                     VEVWIDAQGRARRVQITIVYSGAHLPAGFPANALPQRVESTAEFYDFGTPVSVTVPPA
                     DQAADLAQLAGQGGSRPS"
BASE COUNT          346 a         1048 c         1041 g          394 t
ORIGIN      
        1 cgagaagatc cgcgagcacc tcgccggcgc cgcgccccgc aaggtcgtgg cccgcccgcc
       61 caagctggtc aacatcgtcg tgtgagcccc gaccccgccg tcgagacctg gggcctgagc
      121 cggcgcttcg gcccggtgac ggccctggac ggcctcggca tctcggtggc cgagggcacc
      181 gtgtacggct tcctcggccc caacggcgcc ggcaagacga ccacgatccg gctgctcctc
      241 ggcctgctcg aagcctcggg cggctgggcg cgcgtgctcg gactggaccc ccggaccgcg
      301 ggtgcggagc tgcgccggca gtgcggcgtg ctgctggagc atcccggcct ctacgagcgg
      361 ctgagcgccg aggacaacct cgactacttc gggcggctgt ggcggatccc cgcgggcgag
      421 cggcagctac ggatcgtgga ggtgctggac cacgttgggc tggccgatcg gcgcaccgag
      481 ctggtcggca cgtggagccg gggcatgaag cagaaggtgg ccatcgcccg ggccctgctc
      541 catcgcccgc ggctcgtgtt cctcgacgag cccaccgccg gcctcgatcc cgtcgccgcc
      601 gccgcgttgc gcaacgacct gctctcgctc gtcggtcagg agggcgtgac ggtgttcctg
      661 acgacgcaca acctcgacga ggcccagcgg gtgtgcgacc gcgtcggcgt ggtgcggtct
      721 ggccggctcg tggcccaggg gacgcccgac gagctgcgct ccaccgccgg ccgaccgacc
      781 gtgaccgtgc gcggctcgtc ggtcgatctc gggctggccg cggtggtcgc ccggcccgag
      841 gtggcggggg cccgcatcga cggcgacgcc ctggtggtcg agctgcgctc gcccgggccg
      901 gtggcgccga ttgtgcgcct gctggtcgag gcgggcgtcg ggatcgagga ggtccaccag
      961 ggcgcggcca gcctggagga ggcgttcctc gccctgctgg ccgacccggc ctcggtcgcc
     1021 ccggagccgg ccacgtgatc gccgacgtga tcgccgacgc ggtcaccatc gccggcaagg
     1081 agctcaagga gatcctgagc cgcggcggca cggcgcgggg ccggcggggc atgatcggaa
     1141 cggtggtgcg ccccgcgctg gtcgggctgc tcttcggcct caacatcggc cggggccggt
     1201 cgggggcggc cttggcggtc ctccccgtgg cgttcttcgc catggccacc gcgtcggcgc
     1261 tggtggccga caccatcgcg ggcgagcggg agcggcacac gctcgagacc ctgctggtga
     1321 gcccggcctc cgacgagagc atcctggccg gcaagctcgg cgcggtcgtg ctgtacgcgt
     1381 gggccgtggc gctgatccag ctgctggccg tcgccgtcac cgggatcgcc ctgagcccgg
     1441 cccaccgggt cagcgccggg ctcatcgccg tggttgcgct gttcgccctg ctggaggcca
     1501 ccctcagcgc cgcgctgggc gtgcagttca gcgtgcgggc cgcgaccgtg cgcgccgcgg
     1561 cgcgctccca ggcccaggtc ggcatagtgc tcagcctcct cgccggcggg gccaacttcg
     1621 cggtggccga tccgcgcctc ggcggcccgg tagtcgccgg cgccgccttc gtggtgctcg
     1681 tcgccgccga cgccgttctc ctcggtctgg cccgggcgcg cttccggcgg ggtcggctgc
     1741 tcgtggcctg agtcccgcgc gcccggacgc cgatattggg gagcaaaccc cacatcgtgt
     1801 gttgcgcaat ctccccagtt acggcgaggc tggcggtccc gcctagagtt ggcggcgaac
     1861 gaccggcgag cgaaccaaat ggggaagggg tccgatgggc cgactgcgca gcgcgatcct
     1921 cgtggccacg gcggtgggcg ccgtcctcgc cgtgtccgcc ggcctggccg gttgcggcgg
     1981 aggcggaggg gtgccgccgc tctccatcgt gctggccgcc tcgaccaaca ccgtcgcggc
     2041 caggaccgcc tcggtgcacg tctcgacgat catcaccccc aacgcggcgg cgcccgccaa
     2101 ccccgtcctc aacggccagc cacttgtcgg cgacggcaaa gaggacttcg gggcccacgt
     2161 cgcgaccgtg accgtccagg tgccccgggc ggggagcatc gaggtcgtct attccgggca
     2221 cgtgatctac gagcgcatcc cgcagctggc cgcccagacg ggcaaggcct gggtgaagat
     2281 cgacctggac aacctgggca agcgcgctgg cgtctcgggc ctctccagcc tggcccaggg
     2341 cagcggcggc gacccgagcg agggcctttc ctacttgcgg ggcgccgtcg gggccgtcgt
     2401 caccgccggt cacgagaagg tccgcggtgt gcagaccacc cattacaagg cgaccaccga
     2461 cgtcaaccgg gcggccgccc agctccccgt tgcccagcag gccaccacca agcagatctc
     2521 cgatcagttc gggatcacga ccatcccggt cgaggtgtgg atcgacgccc agggccgggc
     2581 ccggcgggtc cagatcacga tcgtctactc gggcgcccat ctcccggccg gcttcccggc
     2641 caacgccctg ccccagaggg tggagtcgac cgctgagttc tacgacttcg gcacgccggt
     2701 gagcgtcacc gtgccgcccg ccgaccaggc ggccgacctg gcccagctgg ccggccaagg
     2761 tggctcgcgg ccgagctgag cgggctcagg cctccttgaa ggcggcgagc atgttgcgcc
     2821 accgctgag
//
LOCUS       DASWGG010000440         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2500446, whole genome shotgun sequence.
ACCESSION   DASWGG010000440 DASWGG010000000
VERSION     DASWGG010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2500446"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VF954_08065"
     CDS             complement(<1..499)
                     /locus_tag="VF954_08065"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9531079.1"
                     /translation="MTAGSPSQRRRVAILAGHRAELGAARQLLGDEDPAVRAAALGAL
                     ARAGGLTAEELQGACLDPSPLVRRRAAQEAGSGTVGAAAPGGPRSAARALPELPELLD
                     DEDPMVVEMAAWALGERGDRASVGRLSIITMGHDDPLCREAAVAALGAIGDQAGLAAI
                     LAATSD"
     gene            544..1197
                     /locus_tag="VF954_08070"
     CDS             544..1197
                     /locus_tag="VF954_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX9531080.1"
                     /translation="MDGSTKKVVVAVLAGLLVAVGGGVWYGRPVHAGQSAGDDPQPVH
                     SQRVHLTIPPSAPTTSTTLSAEERAFPQPARPIDVPDDPYAPEAVVKIGTIQIPKIGL
                     VADIYQGVTLRNIDNGPSHWPGTALPGEPGNAVLAGHRVTHTHPFRDIDQLVPGDKVI
                     FTVAGVVSTYVVTGHEVVDPSDMAVVAQTPTATATLFACHPPHYATYRYVVHLAAQT"
     gene            1286..1651
                     /locus_tag="VF954_08075"
     CDS             1286..1651
                     /locus_tag="VF954_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531081.1"
                     /translation="MPHMVIFRSVEGKPGYHQAETLDDAVRFVERLRNQEQIPEMRIF
                     RMQEVPIEFKPYYRVEIMGTGEEPTAAGGPSAAGLVAPVADPDEKPDTAAPQAPVASE
                     PVLSNQNGGSARFGRFNRT"
     gene            complement(1687..>2088)
                     /locus_tag="VF954_08080"
     CDS             complement(1687..>2088)
                     /locus_tag="VF954_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531082.1"
                     /translation="PVAAPPVAALPVAPPPVASPPVAAPPVAALPVAPPPVASPPVAP
                     PLMGPPSLGPPPVPVPVRAPPVPAPAAAPARSAAHPRPRPRLAPDIGPVERARSGVLL
                     LVMAVVIGALVAGLLTVLLFLAGLALSSAVG"
BASE COUNT          308 a          787 c          755 g          238 t
ORIGIN      
        1 tgtccgaggt ggcggccagg atcgccgcca acccggcctg gtcgccgatg gccccgagcg
       61 ccgcgacggc cgcttcccgg cacaacgggt catcatggcc catggtgatg atcgacagcc
      121 ggccgaccga tgcccggtcg ccccgctcgc cgagggccca cgccgccatc tcgaccacca
      181 tcgggtcctc gtcgtcgagc agctcgggca gctcgggcaa cgcgcgcgcc gcgctccggg
      241 gcccgccggg agcggccgcc ccgaccgtgc cgctgccggc ctcctgggcc gcccgccggc
      301 gcacgagcgg ggagggatcg aggcacgctc cctgcagctc ctccgccgtg aggccgcccg
      361 cccgggccag cgcgccgaga gcggcggccc gcacggcagg gtcctcgtcg ccgaggagct
      421 ggcgggccgc gcccagctcg gcgcggtggc cggccaggat ggcgacccgg cggcgctggc
      481 ttggcgatcc ggcggtcatt ccggcaccat cggtgccggt cggacgatga tctgggcatg
      541 gtgatggacg gctcgacgaa gaaggtcgtg gtggccgtgc tggcggggct gctggtcgcg
      601 gtgggaggtg gcgtctggta cggccggccc gtgcacgccg gccagtcggc gggcgacgac
      661 ccgcagcccg tccactccca gcgcgtccac ctgacgatcc ctccgtccgc cccgaccacc
      721 tcgaccacgc tgtcggccga ggagcgcgcc ttcccccagc cggcccggcc gatcgacgtg
      781 cccgacgacc catatgcgcc cgaggcggtc gtgaagatcg gcaccatcca gatcccgaag
      841 atcggtctgg tcgccgacat ctaccagggt gtgacgctgc gcaacatcga caacgggccc
      901 agccactggc cgggcacggc ccttccgggg gagccgggca acgccgtgct ggccggtcat
      961 cgggtgaccc atacccatcc cttccgcgac atcgaccagc tcgtgccggg cgacaaggtc
     1021 atcttcaccg ttgccggcgt cgtgagcacc tacgtcgtga ccggccacga ggtggtcgac
     1081 ccgtcggaca tggcggtggt ggcccagacc cccacggcga cggcgacgct cttcgcctgc
     1141 cacccgccgc actacgccac ctaccggtac gtggtgcatc tggcggccca aacctgacat
     1201 acccgacggg agatccccga cctcggggct tccctcaagc tggctcccgc ctcgtccgat
     1261 gcaactcgac ggaaaggagc tggtaatgcc gcacatggtc atcttccgaa gcgtggaagg
     1321 caagcccggg taccaccagg cagagactct ggacgacgca gtccggttcg tcgagcgcct
     1381 gcggaaccag gagcagatcc cggagatgcg catcttccgc atgcaggagg tgcccatcga
     1441 gttcaagccc tactaccgcg tcgagatcat gggcaccggc gaggagccca ccgccgccgg
     1501 cggtccctcc gcggcgggct tggtcgctcc ggtggccgac cccgatgaga agccggacac
     1561 cgccgccccg caggcccccg tggcatccga gccggtcctg tcgaaccaga acggcggctc
     1621 ggcccgcttc gggcgcttca accggacctg agcagtccct cgccacagtg ggctgaggtg
     1681 ggctgatcag cccaccgcgg acgacaacgc cagcccggcg aggaacagca ggacggtcag
     1741 cagcccggcg accagcgcgc cgatcacgac cgccatcacc aggagcagca cgcccgagcg
     1801 ggcccgctcg accggtccga tgtccggcgc caggcgtggt ctgggccgcg ggtgggccgc
     1861 ggatcgggcc ggcgccgccg ccggcgccgg cacaggcggc gcccgtaccg gcaccggcac
     1921 aggcggcggg cccagcgatg gcgggcccat caggggcggg gcgaccggcg gcgaggcgac
     1981 cggaggcggg gcgaccggca aagcggcgac cggcggagcg gcgaccggag gcgaggcgac
     2041 cggaggcggg gcgaccggca aagcggcgac cggcggagcg gcgaccgg
//
LOCUS       DASWGG010000441         4300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2501047, whole genome shotgun sequence.
ACCESSION   DASWGG010000441 DASWGG010000000
VERSION     DASWGG010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2501047"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            841..1200
                     /locus_tag="VF954_08085"
     CDS             841..1200
                     /locus_tag="VF954_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX9531083.1"
                     /translation="MLRWEHTFAADLLGPEASRLLGSLIGEYDRRQGWAADGMASMTD
                     WVAARLALARSTASAWVGAAARLEQLPELRALAAPGGLSFDQLVPLLALAATRLFGPD
                     WDPGSRPSRPPTPPSRN"
     gene            1302..1703
                     /locus_tag="VF954_08090"
     CDS             1302..1703
                     /locus_tag="VF954_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9531084.1"
                     /translation="MLVVDDDAVNRLLLSRLLARMGVNQLRVEGQADRLGDIVRDFQP
                     DLILMDLHVGDVDGLDALREVAAQDPDWSARRVIMITGETGDQIERRCRDAGVSSVLT
                     KPYTAELLLSTVEEALASLPGHERRAPPTAG"
     gene            1832..2881
                     /locus_tag="VF954_08095"
     CDS             1832..2881
                     /locus_tag="VF954_08095"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX9531085.1"
                     /translation="MVTNAMDKGRQLEEHVAAYFRSHGYDVRRNHVLRGRSGAAHEID
                     VLAERSDPLTTVKIAIECKAWETAIEKDVVSKLDMELRDLGLNKGIIVSLSGHSTGAG
                     SVARELNIDLWGPLELESHLGPTLLSDLRVGPATRPSVGFPYRVGEAPARRIVDRQTK
                     GRLGLRAVEDVTWFAPVWLPAWLACLSVSEAGRRRGRTRHAARQVWNLYEAVTGAYLL
                     PAAEPGRGVVPIDVAAGAVRPVVKETAIAQEIRREMERLRKLRSEVAVERHQEALAAL
                     GVPVPCVDVIVESTELVHLPAYLGFLRQRDEQRVVVVTGWAGQFAPHLSAILTSRMTH
                     VRASLGPPAPRPIPR"
     gene            2902..4275
                     /locus_tag="VF954_08100"
     CDS             2902..4275
                     /locus_tag="VF954_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9531086.1"
                     /translation="MATAPVDPAVLAELSAIVGPSHLLVDPDLRAAYETDWTGRFQGR
                     APAVALPASTPEVEAIATACRLHGVALVPQGGNTGLVGGSVPLDGEVVLNLTRMATIE
                     PVDEAAGQLTAGAGAVLADVQAAAGRAGLRYAVDFAARGSATVGGSVASNAGGLNVLR
                     HGATREQLLGVEAVLGRGETVEHLHGLIKDNTGYHLPGLLCGSEGTLAIVTRARLRLI
                     PRHERRVVALVALGTPQQAVETVAAARAGTETLEAAELFLDEGLALVCEAFGLRPPFD
                     RPHAVYVLLEAAAHHDPTDELAAALAAGPAPEDVAVATDPARQGALWRYREDHTLAIN
                     TLGAPHKLDVTLPPGALADFMDEVPGRVRAVRSEARIWLFGHAGDGNIHVNITGVAPD
                     DDAIDELVLLYTAGLGGSISAEHGIGTAKKQWLHLNRSVAEIDAMRAIKAALDPDGIL
                     NPNVLLP"
BASE COUNT          617 a         1533 c         1540 g          610 t
ORIGIN      
        1 cgcgccgatc gtccaccgct ggcctccgtt gcgtgaacga gagtcgctaa cggggtccgg
       61 gtcgaacgca tgcccggcac ccaagcaccg agcctctcaa ccccaacctt acccgcgttg
      121 ggcaccgcca agtccccgcc gacgccgacg ggcgaaacgg gtttgcaggc aagcgtcagg
      181 atcttcgcgg tccgagccac gatcgccggt cccggcgccg ccgaggtagc tctgcccacc
      241 gtcctcaagg gagtcctcga cggcgttgtt gccactttgc caccaccacg atgaagccga
      301 cgcggcggag ctggcgaccc ggatcgcgaa cgcgacgccc atgagcgtct ccgaggtctt
      361 ctaccttgta cagcccgccc gcggaccccc gatcccgacg accgacccgg gcagaacgcc
      421 ggttggtgga cgcccagtca ggcgggccgc agcagtgctc tgcggggccg ctgggacctc
      481 cgacgtcact gacgcctcca tcgtgctggt cgcacgccgc catgacgcca cggtcatcag
      541 ctccgatcgg gccgatctgg aggccctcga cccaacgatt cccgtggtgg attgctgatc
      601 ccggtcaccc cacggtcacc tgcacagccc catcccagcc gcagagcgaa cctccaggcg
      661 cgaccgccat ctcgcgaacc acctacttcg gcgataagcg tccgccgccg ctcatcgttg
      721 cttctgtaaa aacgagcact cgctcggggt gagtggccca accgtcgcgc caccttcttc
      781 tggtggtccg caggttcatg ggaaatttcc gccccttgcc ccttgcatct cgcggcgagg
      841 atgctacgat gggaacacac gttcgcagcg gatcttctcg ggcccgaggc gagtcgtctc
      901 ctgggatcgc tgattggcga gtacgaccgc cggcagggat gggccgccga cggcatggcc
      961 tcgatgaccg actgggtggc ggcgcgcctg gcgctggcgc gctccacggc cagcgcctgg
     1021 gtgggcgcag ccgcccggct ggagcagctg cccgagctgc gggcgctggc cgcccccggg
     1081 gggctgtcct tcgaccagct ggtgcccctg ctggccctgg ccgccaccag gctgttcggg
     1141 cccgactggg accccggcag ccgaccatcc cggccaccca caccgccgag ccggaattga
     1201 ggggccggcg ggcaggcggc gagggataat gggcccgagg ggcgtcgagg tgtccgcggg
     1261 aggtggctgt ggtagcacag atccgggggg ccgacgccag ggtgctggtg gtcgacgatg
     1321 acgcggtcaa ccgcctcctg ctgagccgcc tgttggcgag gatgggagtg aaccagctac
     1381 gggtcgaggg gcaggcggac cgtctcggcg acattgtgcg cgactttcag ccggacctga
     1441 tcctgatgga cctccatgtg ggtgacgtgg acggtttgga cgcgctcaga gaggtggccg
     1501 cccaggaccc ggactggtcg gcgcggcggg tgatcatgat caccggcgag accggcgatc
     1561 agatcgagcg ccggtgccgc gatgccggtg tgtcgagcgt gctcaccaag ccgtacaccg
     1621 cggagctgct gctgagcacg gtcgaggagg cgctggcctc cttgcccggc cacgaaaggc
     1681 gggctccgcc aaccgccggc tgagctctgc cggcgcggcc ccgtgcgccg acatacaggc
     1741 aacgtttcgc cggcggttca gctgcggccg gatcggctcg gatccttgcg cgtcggcgtg
     1801 taccgtcgag ggggcgggcg ctggaggcgg tgtggtcacg aacgccatgg acaagggtcg
     1861 tcagctcgag gagcacgtgg cggcgtactt tcgcagtcac ggctacgacg tgcggcgcaa
     1921 ccacgtcctg cgggggcgct cgggagccgc ccacgagatc gacgtgctgg cggagaggtc
     1981 cgacccgctg acgacggtca agatcgccat cgagtgcaag gcatgggaga cggccatcga
     2041 gaaggacgtg gtctccaagc tcgacatgga gctgcgtgac ctggggctga acaagggcat
     2101 catcgtcagc ctgtccggcc actcgaccgg cgccgggtcc gttgcccggg agctcaacat
     2161 agacctgtgg ggaccgctcg agctggagag ccacctcggc cccacgctgc tctctgacct
     2221 cagggtgggg cccgcgacca gaccgagcgt cggctttccc tatcgggtcg gtgaggcacc
     2281 ggcacgccgg atcgtcgacc gccagaccaa ggggcgcctc gggctgcggg ccgtcgagga
     2341 cgtgacctgg ttcgctcccg tgtggttgcc ggcctggctg gcgtgcctga gcgtgtccga
     2401 agccggccgc cggcgtggcc ggacccgtca cgccgcccgc caggtttgga acctctacga
     2461 ggcggtcacg ggcgcctacc tcctgccagc agctgagccg ggacgaggtg tcgttcccat
     2521 cgacgtcgcg gcgggggcgg tccggccagt ggtgaaggag accgccatag ctcaggagat
     2581 ccgcagggag atggagcggc tccggaagct caggagcgag gtcgcggtcg aacgtcacca
     2641 ggaggcgctg gccgccctcg gtgtgcccgt cccctgcgtg gatgtgatcg tcgagtcgac
     2701 ggagctggtc catctcccgg cgtacctggg cttccttcga cagcgcgacg agcagcgggt
     2761 cgtggtggtg acgggatggg cgggccagtt cgccccccac ctctcggcca tcctcacctc
     2821 ccggatgacc cacgtccggg cgtcgctggg cccgcccgcc cctcgcccga tcccacggtg
     2881 acccggcagt agggtgccgg ggtggccacg gccccggtcg accccgctgt gctggccgag
     2941 ctgtccgcca tcgtcggacc gtcccacctc ctcgtcgatc ccgacctccg ggccgcctac
     3001 gagacggact ggacggggcg cttccagggc cgggccccgg ccgtcgccct tccggcatcc
     3061 acccccgagg tcgaggcgat cgcgacggcc tgccggctgc acggcgtggc gctggtgccg
     3121 cagggcggca acacgggcct ggtcgggggc agcgtgccgc tcgacggcga ggtggtgctc
     3181 aacctgaccc ggatggcgac catcgagccc gtcgacgagg ccgccggcca gctcaccgca
     3241 ggggcgggcg ccgtgctggc cgacgtccag gcagccgcgg gccgcgccgg cctgcggtac
     3301 gcggtcgact tcgccgcccg gggctcggca accgtcgggg ggtcggtcgc ctccaacgcg
     3361 ggcggcctca acgtcctgcg ccacggcgcc acgcgtgagc agttgctggg agtagaggcg
     3421 gtgctcgggc gcggcgagac cgtcgagcac ctccacggcc tgatcaagga caacaccggc
     3481 taccacctgc ccgggctgct gtgcggcagc gagggcacgc tcgccatcgt cacccgggcc
     3541 cggctgcggc tcatcccgcg ccacgagagg cgcgtcgtgg ccctggtcgc tctcggtacg
     3601 ccgcagcagg ccgtcgagac ggtcgcggcg gcccgggcgg ggacggagac gctcgaggcg
     3661 gcagagctct tcctggacga gggtttggca ctggtgtgcg aggccttcgg gctgcggcca
     3721 ccgttcgacc gtccccacgc ggtctacgtg ctgctggagg cggcggccca ccacgacccg
     3781 accgacgagc tggccgcggc gctggccgcc ggccccgcgc cggaggacgt ggcggtggcg
     3841 accgacccgg cccgccaggg cgcgctgtgg cgctaccgcg aggaccacac cctcgccatc
     3901 aacactctgg gcgcgcccca caagctcgac gtgaccctgc cgcctggcgc cttggcggac
     3961 ttcatggacg aggtgccagg ccgcgtccgg gcggtgcggt cggaggccag gatctggctc
     4021 ttcggccatg ccggagacgg caacatccat gtcaacatca cgggcgtggc gcccgacgac
     4081 gacgccatcg acgagctcgt gctgctctac acggcggggc tgggtggaag catcagcgcc
     4141 gagcacggga tcgggaccgc caagaagcag tggctgcacc tcaaccgttc ggtggcggag
     4201 atcgacgcca tgcgcgccat caaggccgcc ctcgacccgg acgggatcct caaccccaac
     4261 gtgctgcttc cctgaaaggg cccggccggg gccccacccc
//
LOCUS       DASWGG010000442         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2503495, whole genome shotgun sequence.
ACCESSION   DASWGG010000442 DASWGG010000000
VERSION     DASWGG010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2503495"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VF954_08105"
     CDS             <1..621
                     /locus_tag="VF954_08105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9531087.1"
                     /translation="GDPDAGPAARRRLGFGAADFVVLCFGFIAPYKGLETALEAARLA
                     GPPVRLVVAGGEHPRLRGREPYVGGLRERWGAWARFTGPVPDAEVAAWFAAADVALLP
                     YPRPFSSSGVLADALAHRTPVLVSPALAACIGAAPECTAPAEPSDLADRLRALAGSRS
                     RLAALQAGAALLARGRSWPSVAHRHLELYEEVTLGAGSSGRCLRAG"
     gene            581..1774
                     /locus_tag="VF954_08110"
     CDS             581..1774
                     /locus_tag="VF954_08110"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX9531088.1"
                     /translation="MEPVLLVGAFGQGNPGDEALFEAFRSALDGWPLVVTSGPFTTRD
                     VAARDVAARDTLGGGALEVRRVPSSSPASVALETARCGAVVFGGGTVFKTLHPSSRRP
                     ARDLVVRALLLAAGARAMGRPVAMMGVGVGDLGGVGTRSLARMLARQATLLLLRDERS
                     AAALGAVGLPGPFRVGSDPAWAVFDTLPGPGPAALGDPGPAGPCERAEAVGLVLNHEM
                     DVREDALVAALTPFVADGRRVLVIPWQTGPFGRGEQDTAERLARRLGGPALATAPPAD
                     LAAATKLLGECAVVLTQRFHALHAAAAAQVAAVAFDHEHKIGALAARLGQRTVAPAAA
                     PAEVAAAAAEAMSSPPPPLPAVRAEIARAQDGFRLLRLLLDPSRLDDLAGLEGLPLEA
                     APSMS"
     gene            1771..>2593
                     /locus_tag="VF954_08115"
     CDS             1771..>2593
                     /locus_tag="VF954_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531089.1"
                     /translation="MTLLAEPLERGPSPVGRRADLTAAVREQWSVAGGQLAAGAGNLA
                     FVLLLARILPPHAFAQITAWLALFLLVNLPATSLGAGAALDPSTATRLARRSAVAGAG
                     VGLGLILSAPLLTTLVGLPAGLVVALGLVAPLAGPVAVARGRLYGREHRRLVASLVVE
                     PAVRLAVGGVLAAVAGAPGAVAGVLIAGAAALGVATFGGVASPGRRSHPAPLARLRQS
                     STRPWATPASPSSASSTGPTLVVFLLLAVIQNQDVVFANAVLRGNHAGTFAALSTL"
BASE COUNT          226 a         1051 c          937 g          379 t
ORIGIN      
        1 ggcgaccctg acgccgggcc cgccgcccgc cgccgcctcg ggttcggtgc cgccgacttc
       61 gtcgtgctgt gcttcggctt catcgccccc tacaagggtc tggagacggc gctcgaagcc
      121 gcccggctgg cgggcccgcc cgtgcgcctc gtcgtcgcgg gaggcgagca cccccgcctt
      181 cggggccgtg agccctacgt gggcggcctc cgggagaggt ggggcgcttg ggcccggttc
      241 accggaccgg tccccgatgc ggaggtggcc gcgtggttcg cggccgccga cgtggcgctc
      301 cttccctacc cccgcccctt ctcgagcagt ggcgtcctcg ccgacgcgct ggctcaccgc
      361 acccccgtgc tcgtgtcgcc ggcgctggcc gcgtgcatcg gcgcggcccc ggagtgcacc
      421 gcgcccgccg agccttccga tctggccgac cgccttcgcg ccctggccgg gagccggtca
      481 cgtctcgccg ccctccaggc cggtgccgcc ctcctggccc ggggccgctc ctggcccagc
      541 gtggcgcacc gccacctcga gctctacgag gaggtgaccc ttggagccgg ttcttctggt
      601 cggtgccttc gggcagggta accccggcga cgaggccctc ttcgaggcct tccggtcggc
      661 cttggacgga tggcccctgg tggtcaccag cgggcccttc accacccggg acgtcgctgc
      721 ccgggacgtc gctgcccggg acaccctcgg gggcggcgcc ctcgaggtgc gcagggtgcc
      781 ctcgtcctcg ccggcgtcgg tggccctcga gaccgccagg tgtggggccg tggtgttcgg
      841 cggcggcacc gtgttcaaga ccctccaccc gtcctcgcgg cgcccggccc gggatctcgt
      901 ggtccgcgcc ctcctgctgg ccgcgggcgc gcgggccatg ggccgccccg tggccatgat
      961 gggcgtgggc gtgggggacc tgggcggggt cgggacccga agcctggccc ggatgctggc
     1021 ccggcaggcc accctcctgc tgctgcgcga cgaacggtcc gccgccgccc tcggcgcggt
     1081 cggcctcccc ggccccttcc gagtgggatc ggacccggcg tgggccgtgt tcgacaccct
     1141 gcccggcccg ggcccggccg cgctgggtga tccgggcccg gcggggccgt gcgagcgggc
     1201 cgaggcggtc ggcctggtcc tcaaccacga gatggacgtc cgcgaggatg ccctggtcgc
     1261 ggcgctcacc cccttcgtcg ccgacggccg gcgggtcctg gtcatcccgt ggcagaccgg
     1321 tccgttcggc cgtggagagc aggacacggc cgaacgcctg gctcgacgac tgggcggccc
     1381 ggcgctcgcc acggcacctc ccgccgacct ggccgcggcc acgaagctcc tcggtgagtg
     1441 tgcggtggtg ctcacccagc ggtttcacgc cctccacgcc gccgccgccg cccaggtggc
     1501 ggcggtcgcc ttcgatcacg agcacaagat cggcgccctg gccgcacggc tcggccaacg
     1561 gacggtggcg ccggccgcgg cgccggccga ggtggccgcg gcggcggccg aggccatgag
     1621 ctcgccacct ccgccgctgc cggctgtccg agccgagatc gcccgggccc aggacggctt
     1681 ccggctgctt cgcctgctgc tcgacccctc ccgcctggac gacctggccg gtctcgaggg
     1741 gctgcccctc gaggccgccc cgtcgatgtc atgacgctgc tggccgagcc cctcgagcgg
     1801 ggtccgtcgc ccgtaggacg gcgggcggac ctcacggctg ccgttcgcga gcagtggtcc
     1861 gtggcgggcg gccagctggc cgccggggcg gggaacctgg ccttcgtcct gctgctcgca
     1921 cgcatcctcc ctccccacgc cttcgcccag ataacggcat ggctcgccct cttcctgctc
     1981 gtcaacctcc cggccaccag cctcggtgcg ggcgcggcgc tcgacccgtc gactgccacg
     2041 cgcctggccc ggcgctccgc cgtcgccggt gccggagtcg gtctcggctt gatcttgtcg
     2101 gcaccgctct tgaccaccct cgtcggcctg cccgcagggc tcgtcgtggc actggggctg
     2161 gtggccccgc tggccggccc tgtggccgtc gcccgcggcc ggctgtacgg acgcgagcat
     2221 cgccggctcg tggccagcct ggtggtcgag cccgccgtca ggctggccgt cggcggcgtg
     2281 ctcgcggccg tcgcaggcgc gccgggcgcg gtggcaggag tcttgatcgc cggagctgcc
     2341 gccctggggg tcgccacctt cggcggggtc gcctcccctg gccgccgctc ccaccccgcc
     2401 ccgctggcgc ggctccgcca gtcgtcgacc cgcccctggg ccacccccgc gagcccgtcg
     2461 agcgcctcga gcacgggccc gactctggtc gtcttcctcc tgctggccgt catccagaac
     2521 caggacgtgg tgttcgccaa cgccgtgctt cgcggcaacc acgcggggac cttcgccgcc
     2581 ctctccacgc tgg
//
LOCUS       DASWGG010000443         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2503645, whole genome shotgun sequence.
ACCESSION   DASWGG010000443 DASWGG010000000
VERSION     DASWGG010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2503645"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VF954_08120"
     CDS             complement(<1..432)
                     /locus_tag="VF954_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9531090.1"
                     /translation="MPKSVASPWWGDGGTPLPAASVAVEVPADAEVLGVPVLSGPDGA
                     VVAGEALVELDLVWLAGRGFEGKPGDLQVVPADDGSVVVAVGVGEAGTVDRERWRKAG
                     AATVRAGGTARRLAFAIPLSLLGAKGEGAAAAAQAVVEGAAL"
     gene            complement(434..988)
                     /locus_tag="VF954_08125"
     CDS             complement(434..988)
                     /locus_tag="VF954_08125"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX9531091.1"
                     /translation="MTPAERFYTGKGDDGTTGLLYGGRTSKGSPVIEANGAIDEAQAA
                     IGLARASAPRHGELDELLVHVERDLWIMMAEMATAPENRRKLQAGQTLVTEEMVGWVS
                     SATDDLSGRFAMPHEFVIPGQDRLSAELDVARTVVRRAERLAVGVAAPGSWMIPYLNR
                     LSSLLWAAARWQEGEALVSRPRED"
     gene            complement(985..>2266)
                     /locus_tag="VF954_08130"
     CDS             complement(985..>2266)
                     /locus_tag="VF954_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9531092.1"
                     /translation="RFLALGGADLDARAAVVCRDVSLPADRLDVPVGALSGGQAARAA
                     LAAILLARFDVFLLDEPTNNLDFAGLDRLERFIAELDGGVVVVSHDRAFLDHAVHRIV
                     EIQEQHHTAVEYAGGWSDYVAARALARSQQHQAYEATTRERARLTERLRTQRSWSEQG
                     AAAARRKPKDNDKAQRGFSINRTEKQASKVRATERRLAHLGAVDKPWEGWELRLDLGS
                     SARGGDVVVRLEGAVVSRGAFRLGPVDLEVGWQERVAILGPNGSGKTTLLGAMLGTLP
                     LAAGRRYLGPGVVVGAMEQGRGQLSASVPAGDLAADFCRRTGLPSSEARTLLAKFGLG
                     ADHASRPFDQLSPGERTRAVMAALMAEGVNCLVLDEPTNHLDLAAIEQLEAALAAFDG
                     TLLLVTHDRRLLEAVHLDRALELGREGGPKVTGL"
BASE COUNT          307 a          868 c          811 g          280 t
ORIGIN      
        1 aagggcagcc ccttcgacca cggcctgggc cgcggcggcc gcgccctcgc ctttggcgcc
       61 gagcaggctg agcgggatgg cgaaggccag gcgccgggcc gtgccgcccg cccgcaccgt
      121 cgccgcgccc gcctttcgcc accgctcgcg gtccacggtc ccggcctcgc ccacccccac
      181 cgcgaccacg acggacccgt cgtcggcggg gacgacctgc aggtcgccag gcttgccctc
      241 gaacccccgg ccggccagcc acaccaggtc cagctcgaca agggcctcac ctgccacgac
      301 cgcgccgtcc ggcccggaca gcaccggcac ccccagcacc tccgcgtcgg cgggcacctc
      361 gacggccacc gacgcggccg gcaacggcgt gccgccgtcg ccccaccagg gcgaggccac
      421 agacttcggc atttcaatcc tcccggggtc ggctgaccag ggcttcgccc tcctgccaac
      481 gggccgcggc ccacagcagg ctggagagcc ggttcaggta cgggatcatc cacgagccgg
      541 gggcggcgac gccaaccgcg aggcgctcgg cccggcgcac gaccgtccga gccacgtcca
      601 gctccgccga aagccggtcc tggccgggga tgacgaactc gtgcggcatg gcgaagcgcc
      661 cgctgaggtc gtcggtcgcg gacgataccc aacccaccat ctcctccgtc accagcgtct
      721 ggccggcctg gagcttgcga cggttctcgg gggcggtggc catctccgcc atcatgatcc
      781 agaggtcgcg ctcgacatgc acgagcagct cgtcaagctc gccgtgccgg ggagccgacg
      841 cccgggccag accgatggcc gcctgggcct cgtcgatggc gccgttggcc tcgatcaccg
      901 gcgagccctt gctggtgcgg ccgccgtaga gcagcccggt cgtcccgtcg tcgcccttgc
      961 cggtgtagaa ccgctccgcg ggggtcacag gccggtcacc ttagggcctc cctcgcgccc
     1021 cagctccagg gcccggtcga ggtgcacggc ctcgagcagg cggcggtcgt gggtgaccag
     1081 cagcagggtg ccgtcgaagg cggccagggc ggcctcgagc tgttcgatgg ccgccagatc
     1141 gaggtggttg gtcggctcgt ccaggaccag gcagttgacg ccctcggcca tgagggcggc
     1201 catgacggcg cgggtccgct ctcccggcga gagctggtcg aacgggcgcg aggcgtggtc
     1261 ggcaccgagc ccgaatttcg ccagcaacgt gcgcgcctcg ctcgagggca accctgtccg
     1321 gcggcagaag tcggcggcca ggtcacccgc cgggaccgac gccgagagct gcccgcgccc
     1381 ctgctccatc gccccgacga cgacgccggg acccaagtag cgccggccgg cggccagggg
     1441 gagggtcccg agcatggccc ccagcaacgt cgtcttgccg ctcccgttgg gcccgaggat
     1501 ggcgacgcgc tcctgccagc ccacctcgag gtcgacgggg cccagccgga aggcgccccg
     1561 cgagacgacg gcgccttcga ggcgcaccac cacgtcgccg ccccgcgccg aggacccgag
     1621 atcgaggcgc agctcccatc cctcccacgg cttgtcgacg gcgcccaggt gggcgaggcg
     1681 gcgctcggtg gcccggacct tggaagcctg cttctcggtg cggttgatgg agaagccgcg
     1741 ctgggccttg tcgttgtcct tcggcttgcg cctggccgca gccgcgcctt gctcggacca
     1801 cgagcgctgg gtgcggaggc gctcggtcag gcgggcccgc tccctggtgg tggcctcata
     1861 ggcctggtgc tgctgcgaac gggccagcgc ccgggcggcc acgtagtcgg accacccgcc
     1921 ggcgtactcg acggcggtgt ggtgctgctc ctggatctcg acgatccggt ggacggcgtg
     1981 gtcgagaaag gcccgatcgt ggctgacgac caccaccccg ccgtcgagct cggcgatgaa
     2041 ccgctcgagg cggtcgaggc cggcgaagtc caggttgttg gtgggctcgt cgagcaggaa
     2101 cacgtcgaag cgcgccagca ggatcgccgc cagcgcggca cgcgccgcct gcccgccgga
     2161 gagcgcgccc accggcacgt ccaggcggtc cgcgggaagg ctcacatcgc ggcagaccac
     2221 tgccgcccgg gcgtcgaggt cggcaccgcc gagggccagg aaccgt
//
LOCUS       DASWGG010000444         4759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2504581, whole genome shotgun sequence.
ACCESSION   DASWGG010000444 DASWGG010000000
VERSION     DASWGG010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2504581"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2060)
                     /locus_tag="VF954_08135"
     CDS             complement(<1..2060)
                     /locus_tag="VF954_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX9531093.1"
                     /translation="MRRCIRALAGAALLLAVLSASAATAAAHAPRRQVPFRSPEAGSG
                     ASSAGRPGAPFAPETVRLAGAAGPGVKPRAVATPSSFPLISLEDELNVADVSPPDTQV
                     AAGPTELVEMVNVTLSTWDKSGRRTSGPASLDSVFDVPPGLDFTDPRVVFDPANGGRF
                     FASGSGINFSGGPGTTSQTYVAVSTSSAPTSSPADWLHFTVASNSGGTIYDQPKLGVT
                     SDKVVITWSDFAGASLPAEEIWVVEKGDLIAGTATAASEAEVGTPSTARFSVVPAVDE
                     LGTEGPDAWLVYNAADPQLAAEPGTPSAGVLEITGRPATHDTTLALARFSPIAATSLP
                     PPADEPSKSAEIDTGDDRFLSAVISGGRVWAGGTDACTPAGDTTVRPCLRLVELAVGA
                     APATLQDFDAGQAGTDWYFPAPAFNGAGDLIVSFSSSSPVQLPSAAASGQLRSDTNRL
                     DPETIVLAGQTVYTESGANCPASADASAGQASRWGDYSSAAVDPTDGTVWTAAEYAAA
                     TSDARHSSCDWGTGAARVTFGAPVVGGVAPNRGPLDRPTPVVLTGTDFGPDSTVTFGS
                     VPSASVTVLSSTRISAVAPASAVEAVVPVTVSDRYGTSAPGGPYRYDAGAGYWFVAAD
                     GGVFAFGDAGFFGSTGSLRLNAPIVGMAPTPSGHGYWFVASDGGVFAFGDAGFFGSTG
                     SLRL"
     gene            complement(2143..2340)
                     /locus_tag="VF954_08140"
     CDS             complement(2143..2340)
                     /locus_tag="VF954_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531094.1"
                     /translation="MTTDLDLGVVRWWDRGPARDILYASACLPGLFPAITDFRRTVRL
                     ISESREIARAFLARSAATASA"
     gene            complement(2346..2465)
                     /locus_tag="VF954_08145"
                     /pseudo
     CDS             complement(2346..2465)
                     /locus_tag="VF954_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010332599.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            2625..3269
                     /locus_tag="VF954_08150"
     CDS             2625..3269
                     /locus_tag="VF954_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9531095.1"
                     /translation="MIELGLRNDVYRRPLATALDRLGLREGWRGVDVGAGGGDVSVAL
                     AEIVGRDGRIYAVDSDPAARDEVARAAAAHAQVVAITQGAEDLTLPEEVDLAFCRFLL
                     LHVVEPVRVLDRMGRAVRKGGWVVAQEPITSAGRIDGRPFSMPDAPHPDVGALLPALV
                     LQAGLEIVDAWAEAPAGAGPGPLADYLEALTGVHPGNDPVVLPPLVTVVARRPG"
     gene            3334..3624
                     /locus_tag="VF954_08155"
     CDS             3334..3624
                     /locus_tag="VF954_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531096.1"
                     /translation="MVERAPSGMQRFTMWRETDASGVSGTGLVLEGVVFSTGVVVAHW
                     LTPAPRGSISLFDSLEQFLSIHVEPHPGNRTLITFDDGATIDHHDALIVLRH"
     gene            3657..>4759
                     /locus_tag="VF954_08160"
     CDS             3657..>4759
                     /locus_tag="VF954_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9531097.1"
                     /translation="MATPDLTAAASAVEVASSVVRNGAVQLASFGPRAADDHQVLAYD
                     LAHAAAAVEIARSALDYGAKGATEAALACAFVADAVYDVMCKVAGREAHWAAADVAWP
                     HDFVAACRDPEWLASLCGEAGPRHLDADFELVQDSFRRFAEEKIEPVAEHVHRHNGDI
                     PEEVIAGLAEMGAFGLSVPEEYGGWATGGESDYLGMVVATEELSRGSLGVGGSLITRP
                     EILTRALVRGGTEEQKRHWLPRLASGELLAAVAVTEPDFGSHVAGITVSATGTDGGWL
                     VNGVKTWCTFAARADVLMLLARTDPDRGKAHRGLSLFVVPKPRGDGHGFELGDGRGGR
                     LEGRPIDTIGYRGMHSYELAFDSWFVGEESLIGG"
BASE COUNT          661 a         1705 c         1785 g          608 t
ORIGIN      
        1 ttcagccgca ggctcccggt cgagccgaag aagccggcgt caccgaaggc gaacaccccg
       61 ccgtcggacg ccacgaacca gtacccgtgc cccgaaggcg tcggcgccat gcccactatg
      121 ggcgcgttca gccgcaggct cccggtcgag ccgaagaagc cggcgtcacc gaaggcgaac
      181 accccgccgt cggccgccac gaaccagtac cccgcgcccg cgtcgtagcg gtagggccca
      241 ccgggcgcgc tggtcccgta gcggtccgag accgtgacgg gaacgacggc ctcgaccgca
      301 gaggcggggg ccacggcgga gattcgagtg gaggacagga cggtcacgga cgcgctcggc
      361 acggagccga aggtgaccgt cgaatcgggc ccgaagtcgg tccccgtcag gaccacggga
      421 gtcggccgat cgagcgggcc ccggttgggc gccacgccgc ccaccaccgg cgccccgaag
      481 gtcacgcgag ccgcgccggt gccccagtcg cacgaggagt gcctggcatc ggaggtggcc
      541 gcggcgtact cggcggcggt ccagacggtg ccgtcggtgg gatcaaccgc ggccgacgag
      601 tagtcgcccc agcgcgaggc ctggccggcg gaggcgtccg cgctggccgg gcagttggcg
      661 ccgctctcgg tgtagaccgt ctggccggcg aggacgatcg tctcggggtc gaggcggttg
      721 gtgtcgctgc gcagctgccc ggacgcggcg gcggagggaa gctggaccgg cgaggagctg
      781 ctgaaggaga cgatgaggtc tccggccccg ttgaaggcgg gggcggggaa ataccaatcg
      841 gtgccggcct ggccggcgtc gaagtcctga agcgtcgcgg gcgcggcgcc gacggccagc
      901 tcgaccagcc gcaggcacgg ccgcaccgtg gtgtccccgg cgggcgtgca cgcgtcggtc
      961 ccgcccgccc acacccggcc gccgctgatg accgcgctca ggaagcggtc gtcgccggtg
     1021 tcgatctccg cggacttgga cggctcgtcg gcgggcgggg gcagtgacgt cgccgcgatc
     1081 gggctgaacc tggcgagggc cagcgtcgtg tcatgggtgg ccgggcggcc cgtgatctcc
     1141 agcactccgg ccgagggcgt acccggctcg gccgcgagct gcgggtcggc ggcgttgtag
     1201 accagccagg cgtcgggacc ctccgtgccg agctcgtcga cggccggcac cacgctgaag
     1261 cgcgccgtcg agggggtgcc gacctcggcc tcggacgcgg cagtggccgt gcctgctatt
     1321 aggtcgcctt tctccaccac ccatatctcc tcggcgggca ggctggcgcc ggcgaagtcg
     1381 ctccaggtga tcaccacctt gtcggaggtc acgccgagct tgggctggtc gtagatcgtg
     1441 cccccgctgt tcgaggcgac ggtgaagtgg agccagtccg ccggcgagga ggtcggggcg
     1501 gacgaggtgg acaccgccac gtacgtctgg ctggtggtgc cgggcccgcc ggagaagttg
     1561 atcccagagc cggaggcgaa gaagcgcccc ccgttagccg ggtcgaacac gacccggggg
     1621 tcggtgaagt ccaggcccgg ggggacgtcg aacaccgagt ccaacgacgc cgggcccgag
     1681 gtcctccggc cggacttgtc ccacgtcgac agcgtgacgt tcaccatctc gaccagctcg
     1741 gtcggcccgg ccgccacctg cgtatccggc gggctgacgt cggcgacgtt gagctcgtcc
     1801 tccaggctga tgaggggaaa ggacgacggg gtggccaccg cccggggctt cacacccggg
     1861 cccgcggccc ccgccaggcg gaccgtctcc ggcgcgaaag gtgcgcccgg ccggcccgcg
     1921 gacgacgcgc cagatccggc ctcggggctc cggaagggca cctggcgccg cggcgcgtga
     1981 gccgccgcgg tcgcggcgct cgccgagagc accgctagca ggagggcggc gcccgccagg
     2041 gcccggatgc atcgacgcat gcgcccacct tgccagacga acgcgcccga catgcgccag
     2101 cggctcagta gaccgcctgc gccctccgta cggctccggg ggctaggcac tggcggtggc
     2161 cgcggaccga gcgaggaacg cccgggcgat ctcccggctc tcgctgatga gcctcaccgt
     2221 ccggcggaag tcggtgatgg ccgggaagag cccgggcagg cacgcgctgg catagaggat
     2281 gtcgcgggcc ggtccgcggt cccaccaccg caccaccccg agatcgaggt cggtggtcac
     2341 gacgcggcgc ggacccgggc gatcagctcc cgcatgcggt agggcttggt cacgtaatcg
     2401 tcggccccga cctcctgggc gacgacggcg tcgatctcct cacccttggc cgtgaccatg
     2461 atgatcgtca cgcgccgagg gcagtgcgga gtcgctcatg tggcccgcac gctaggggcg
     2521 ccgcaagaac cgcccccaac cgtggtgcga acggaggatg aacctgggcc gaacacttgc
     2581 agccgagctc caccaaggcg ccatggcggt agggtcgggc gccgatgatc gagctcgggc
     2641 tgcgcaacga cgtgtaccgg cggccgctcg ccaccgcgct cgaccgcctg ggcctgcggg
     2701 agggctggcg cggcgtggac gtgggcgccg ggggcggcga cgtgagcgtg gccctggccg
     2761 agatcgtggg gcgcgacggc cgcatctacg ccgtcgacag cgacccggcg gcgcgcgacg
     2821 aggtggcccg ggcggcagcc gcccacgccc aggtggtggc catcacccag ggggcggagg
     2881 acctgaccct gcccgaggag gtcgacctgg cgttctgccg gttcctgctc ctccacgtgg
     2941 tcgagccggt cagggtcctg gaccggatgg gccgcgccgt gcggaagggc gggtgggtcg
     3001 tggcccagga gcccatcacc tcggccgggc gcatcgacgg ccgacctttc tccatgcccg
     3061 acgcaccgca cccggacgtg ggcgccctgc taccggccct cgtcctccag gccgggctgg
     3121 agatcgtgga cgcctgggcc gaggctcccg ccggcgcggg tcctggcccg ctcgccgact
     3181 acctcgaggc gctcaccggc gtccaccccg gcaacgaccc cgtcgtgctg ccgcctctgg
     3241 tcaccgtggt cgcccgccgg ccgggctgac cacgctccga gatggcccgg acgggccatg
     3301 gccaagcatc ggacaagaga ggacaatcgg ggcatggtcg aacgggcccc gtcggggatg
     3361 cagcgcttca ccatgtggcg ggagaccgac gcgtccggcg tatcggggac cggcctggtg
     3421 ctggagggcg tcgtgttctc gaccggcgtg gtcgtcgccc actggctgac acccgcgccg
     3481 cgtgggtcga tcagcttgtt cgactccctg gagcagttcc tctccatcca cgtcgagccc
     3541 catcccggca accggacgct catcacgttc gacgacgggg ccaccatcga ccaccacgac
     3601 gcgttgatcg tgctgcgcca ctgagcgccg agccctgatg ccatagcctc cccgccatgg
     3661 cgaccccaga cctgacagca gccgcctcgg ccgtcgaggt ggcgtcctcc gtcgtgcgga
     3721 acggcgcggt tcagctcgcc tccttcggcc cgcgcgccgc ggacgaccac caggtgctcg
     3781 cctacgacct ggcccacgcc gccgccgcgg tggagatcgc ccggtcggcg ctcgactacg
     3841 gcgccaaggg ggccaccgag gccgcgctgg cgtgtgcctt cgtggccgac gcggtgtacg
     3901 acgtgatgtg caaggtcgcc gggcgcgagg cccactgggc cgcggccgac gtggcgtggc
     3961 cgcacgactt cgtcgccgcc tgccgagacc ccgagtggct ggcgtcattg tgcggcgagg
     4021 ccgggccccg ccatctcgac gccgacttcg agctggtgca ggacagcttc cgccgcttcg
     4081 ccgaggagaa gatcgagccc gtcgccgagc acgtccaccg ccacaacggc gacatccccg
     4141 aagaggtcat cgccgggctg gccgagatgg gcgccttcgg cctgtccgtt cccgaggagt
     4201 acggcggctg ggccactggc ggcgagagcg actacctcgg catggtcgtg gcgaccgagg
     4261 agctgtcgag ggggtcgctc ggcgtcggcg gctcgttgat cacccggccc gagatcctga
     4321 cccgcgccct ggtccggggc ggcaccgagg agcagaagcg ccactggctg ccccgcctgg
     4381 cctccggtga gctgctcgcc gcggtggccg tcaccgagcc cgacttcggc tcgcacgtcg
     4441 ccggcatcac ggtgagcgcg accggcaccg acgggggctg gctggtcaac ggggtcaaga
     4501 cgtggtgcac gttcgcggcc cgggcggacg tgctgatgct gctggcccgg accgatcccg
     4561 accgaggcaa ggcccaccgc ggcctgtcgc tgttcgtggt gcccaagccg cggggcgacg
     4621 ggcacggctt cgagctcggc gacgggcggg gcgggcggct cgagggccgg cccatcgaca
     4681 ccatcggcta caggggcatg cacagctacg agctggcgtt cgacagctgg ttcgtcgggg
     4741 aggaaagcct catcggtgg
//
LOCUS       DASWGG010000445         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2512427, whole genome shotgun sequence.
ACCESSION   DASWGG010000445 DASWGG010000000
VERSION     DASWGG010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2512427"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..474
                     /locus_tag="VF954_08165"
     CDS             139..474
                     /locus_tag="VF954_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX9531098.1"
                     /translation="MGNDTLTDEERAGRLRDVTELVEMIRPAVQADGGDLVLVSADVE
                     TGVVEVMLQGSCSSCAISATTLQAGVNRIMKDRLPWVTEVTGDVDTSIDPEVSAAMGT
                     GAYVPRYEY"
     gene            493..>1990
                     /gene="pepN"
                     /locus_tag="VF954_08170"
     CDS             493..>1990
                     /gene="pepN"
                     /locus_tag="VF954_08170"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX9531099.1"
                     /translation="MLANNLTKADAAARCALLERVSYDVALDLAAGDDTFGVRTDIRF
                     TCRQPGGATFLDLVAESVQSIEVNGETRPLDSFAGDRIVLDGLGSHNHVSIEARCPYD
                     HNGVGLHRFVDPTDGLAYLHTQFEPFDAHRVYACFDQPDIKAPFSLQVTAPAAWTLVS
                     NSPVLERRDGQPGDTRHWRFRATPPISPYLTALVAGPFRAWSSTHGPIALGIFCRQSL
                     AEFLDVEELWDVTHRGLDFFSQQFGRAYPFEKYDQLFVPEFNAGAMENAGCVTFSESY
                     VFRSKITEAARESRANTILHEMAHMWFGDLVTMRWWDDLWLNESFATYMAYLASADAT
                     RFTKAWARFASSMKAWAYAEDQLPSTHPISTDIADTESVRLHFDGISYAKGASVLRQL
                     VAWVGRDGFERGVRHYFGRHEWANATLADFLAALEEASSRPLVGWAKEWLQTAGVNTL
                     RLEVDEDGDHYRSVSIVQTAPHDHPTLRSHRLAVGLYRAGEAGLVRTGR"
BASE COUNT          298 a          742 c          669 g          281 t
ORIGIN      
        1 gctcggacgg ggccgaggcg ctgtgcgagg agctggccgc ggaccgcgag cagcgcctgg
       61 cgtcgagcgc cgtggcgtcg ggcacccgct ccgggaccgg gaacacccgg gcgctcccct
      121 aggttggaac atgcagccat gggcaacgac accttgaccg acgaggagcg agccggccgg
      181 ctccgggacg tgaccgagct ggtcgagatg atccgcccgg ccgtgcaggc cgacggcggc
      241 gacctcgtgc tggtgtccgc cgacgtggag accggcgtgg tcgaggtcat gctccagggc
      301 tcctgctcgt cctgcgccat cagcgccacc acgctgcagg ccggcgtcaa ccggatcatg
      361 aaggaccgcc tgccctgggt caccgaggtg accggcgatg tcgacacctc gatcgacccc
      421 gaggtcagcg ccgccatggg caccggcgcc tacgtcccgc gctacgagta ctgagccact
      481 accgttcggc gcgtgctggc caacaacctg accaaggcgg atgcggcggc ccggtgcgcg
      541 ctgctcgaac gggtgtccta cgacgtggcc ctggacctgg cagcgggcga cgacaccttc
      601 ggtgtccgca ccgacatccg gttcacctgc cgccagccgg gcggggccac cttcctcgac
      661 ctggtcgccg agtccgtcca gagcatcgag gtcaacggag agacccgccc gctcgacagc
      721 ttcgccggcg atcgcatcgt gctggacggc ctgggctctc acaaccacgt ctccatcgag
      781 gcccgctgcc cctacgacca caacggcgtg ggcctgcacc ggttcgtgga cccgaccgac
      841 gggctggcct acctccacac acagttcgag cccttcgacg cccaccgggt gtacgcctgc
      901 ttcgaccagc ccgacatcaa ggcccccttc agcctccagg tgaccgcgcc ggccgcgtgg
      961 acgctcgtga gcaacagccc ggtgctggag cgccgggacg ggcagcccgg ggacacgcgc
     1021 cactggcggt tccgggccac gccgccgatc tcgccctacc tgaccgccct ggtggccggt
     1081 cccttccggg cctggtcctc cacccacggc ccgatcgccc tcggcatctt ctgccggcag
     1141 tcgctggccg agttcctgga cgtcgaggag ctgtgggacg tgacccaccg ggggctcgac
     1201 ttcttcagcc agcagttcgg gcgggcctac ccgttcgaga agtacgacca gctcttcgtg
     1261 ccggagttca acgccggggc catggagaac gcgggctgcg tgaccttcag cgagagctac
     1321 gtgttccggt ccaagatcac cgaggcggcc agggagagcc gcgccaacac gatcctgcac
     1381 gagatggccc acatgtggtt cggcgacctc gtgaccatgc ggtggtggga cgacctgtgg
     1441 ctcaacgaga gcttcgcgac ctacatggcg tacctggcct cggcggacgc cacccgtttc
     1501 accaaggcgt gggcccggtt cgccagctcc atgaaggcct gggcctacgc cgaggaccag
     1561 ctgccgtcga cccaccccat ctccaccgac atcgccgaca ccgagtccgt ccggctgcac
     1621 ttcgacggca tcagctacgc caagggggcc tcggtcctgc gccagctggt ggcctgggtc
     1681 ggccgggacg gcttcgagcg gggcgtgcgg cactacttcg gccgccacga gtgggccaac
     1741 gccaccctgg ccgacttcct cgccgccctc gaggaggcgt cgtcccggcc gctcgtgggc
     1801 tgggccaagg agtggctcca gaccgcgggg gtcaacaccc tgcgcctgga ggtcgacgag
     1861 gacggcgacc actaccgcag cgtcagcatc gtgcagaccg cgccgcacga ccatccgacc
     1921 ctgcggtccc accgcctcgc cgtgggcctg taccgggccg gcgaagctgg cctggtccgc
     1981 accgggcgca
//
LOCUS       DASWGG010000446         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2512494, whole genome shotgun sequence.
ACCESSION   DASWGG010000446 DASWGG010000000
VERSION     DASWGG010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2512494"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..442)
                     /locus_tag="VF954_08175"
     CDS             complement(239..442)
                     /locus_tag="VF954_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531100.1"
                     /translation="MNNIILDTVDAMELIEILEFFMERLDVLAEHDFAKLFFAECRPY
                     GLEDLRADVTRLINSLNLSPLSP"
     gene            complement(658..>1679)
                     /locus_tag="VF954_08180"
     CDS             complement(658..>1679)
                     /locus_tag="VF954_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9531101.1"
                     /translation="RPVTGGVDTHLDVHVAAALDGIGGLLGVESFEASAAGNDKLLCW
                     LQSFGPVGRVGVEGTGSYGAGLARWLRRAEVEVVEVDRPNRQARRRQGKSDPLDAVEA
                     ARAAQAGRAFGAAKTRDGNVEAIRALVVARRSGRSTKIRTLNQIRHLGFTAPDELRER
                     LRGVSRQRLAAEAAALRPRPGGDPVTCATKTALRTLGRRVLALDEETAALDALLAELV
                     TATAPALVAVHGVGVDTAAGLLVAAGDNPDRLRSEAAWAHLCGVAPIEASSGKVTRHR
                     LNRGGDRQANAALWRIVITRMSSDPRTRAYVARRVDEGRSKGEIIRILKRYVAREVYR
                     HLPRG"
BASE COUNT          242 a          563 c          617 g          257 t
ORIGIN      
        1 actcctgtta ctgctcggcg ccttcctctg gttcccgacg gtttccgccc ttgaacggac
       61 ctgagacgga cctggaacgg acctcgttgt accgcgtcat gttggaggcg atcccagccg
      121 aggaagtcga cgtgtccaac cgaagcgccc gcgcggccgt tgcgcccatg gtgaaaggcg
      181 gcacggcttc acgcgctgga tattcgcagc gccgccctaa acgatgctcc tacgttcgtc
      241 aagggctcag tgggctgagg ttgagactgt tgatcaggcg ggtgacgtct gctcgcaggt
      301 cttcgaggcc gtaggggcgg cactcagcga agaagagctt ggcgaagtcg tgctcggcga
      361 ggacatcgag tcgctccatg aagaactcga ggatctcgat gagttccatg gcgtcgacgg
      421 tgtcgaggat gatgttgttc aaaggtcggg tcctttgccg ggttgggcgt cggcggctgg
      481 ggcgcggcag gacagggatg agccactcga gaacgagggc aggctcttac gaggccaaca
      541 tgtcctggcc gctgacgcgg gggtgggctc gtcagcagca ggccggcaga tcgttctcag
      601 gacagcccca ccagggcgtc agccgattat cgggccaggc ctgctgctgt gagcgagcta
      661 gccgcgaggc aggtggcggt agacctcgcg ggcgacgtag cgcttgagga tgcggatgat
      721 ctcgcccttg gagcggccct cgtcgactcg gcgggccacg taggcgcggg tgcgaggatc
      781 ggagctcatg cgggtgatca cgatccgcca cagggcggcg ttggcttggc ggtcgccgcc
      841 ccggttgagc cggtggcggg tcaccttgcc ggaggaggct tcgatggggg cgacgccgca
      901 caggtgggcc caggccgcct cggagcggag ccggtccggg ttgtcgccgg cggcgaccag
      961 caggcccgcc gcggtgtcga ctccgacgcc gtggacggcg acgagggcgg gggcggtggc
     1021 cgtcaccagc tcggccagca gcgcatcgag cgctgccgtc tcctcatcca gggccaggac
     1081 ccggcgtccc agggtgcgca gcgcggtctt ggtggcgcag gtgacggggt cacccccggg
     1141 ccgaggccgc agcgcggcgg cctcggcggc cagccgctgg cgggagacgc cgcgtaggcg
     1201 ttcccgcagc tcgtcgggag cggtgaagcc gaggtggcgg atctggttga gggtcctgat
     1261 cttggtcgac cggccgctgc gcctggccac caccagggcc cggatggcct ccacgttccc
     1321 gtctcgggtc ttggccgccc cgaaggctcg cccggcctgg gcggcccggg ccgcctccac
     1381 cgcgtcgagg gggtccgact tgccctggcg gcggcgggcc tggcggttgg ggcggtcgac
     1441 ctcgaccacc tccacctccg ctctccgcaa ccagcgggcc aggccagcgc cgtaggagcc
     1501 ggtcccctcc acccccaccc gcccaaccgg accgaacgac tggagccagc acagcagctt
     1561 gtcgttcccg gcggccgacg cctcaaagct ctccacgccc aacagtccgc cgatcccgtc
     1621 cagcgccgcc gccacatgaa catcaagatg ggtatcgacg ccgcccgtca ccgggcgcg
//
LOCUS       DASWGG010000447         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2517501, whole genome shotgun sequence.
ACCESSION   DASWGG010000447 DASWGG010000000
VERSION     DASWGG010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2517501"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VF954_08185"
     CDS             complement(<1..376)
                     /locus_tag="VF954_08185"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX9531102.1"
                     /translation="MALGLTIDALVGDPDKWHPVAGFGRLMMAAEERMYRDSRAAGAA
                     YAAVGLGLGVLAGTACPSVSAMTAVVAAGKGLASAAAAVGAALDGGRLDEARRLLPAL
                     VGREPTQLDSRQIARAVVESVAE"
     gene            complement(539..1318)
                     /locus_tag="VF954_08190"
     CDS             complement(539..1318)
                     /locus_tag="VF954_08190"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9531103.1"
                     /translation="MPPSPDLTFDLSDRVAVITGGSRGLGREMALAFADHGATVVVAS
                     RKVEACVSLAADITAKTGRPALGVGCHVGRWEDCDELVRQVVDAYGRIDVLVNNAGMS
                     PLYPSLDAVTEDLFDKVIDVNLKGAFRLMALVGTRMAQGGGGSIVNVSSIAAVQPGVG
                     ELPYAVAKAGLNAATLGMARAFAPTVRVNAIMPGPFLTDISKAWDLPAFEQMAARTIP
                     LGRAGAPPEIVGAALYLASGASSYTTGAVIKVDGGSAWAPG"
     gene            complement(1352..>1613)
                     /locus_tag="VF954_08195"
     CDS             complement(1352..>1613)
                     /locus_tag="VF954_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HEX9531104.1"
                     /translation="AGESVGDLVATCVGIVRASGLPNETNAMFTNVEGDWDEVMGVIK
                     RCVDEVAASAPRVSLVIKVDHRAGVADALHAKVHAVEERLAN"
BASE COUNT          216 a          592 c          591 g          214 t
ORIGIN      
        1 tctctgcgac cgactccacc acggcccggg cgatctgccg gctgtcgagc tgggtgggct
       61 cgcgccccac cagcgcgggc agcaggcggc gggcctcgtc gaggcggccg ccgtcgaggg
      121 ccgcgccgac cgcggccgcg gcggacgcca gccccttgcc cgccgcgacc accgccgtca
      181 tggccgacac cgacgggcag gccgtgccgg cgaggacgcc cagccccagc ccgacggcgg
      241 cataggcggc gcccgccgct ctggagtccc ggtacatgcg ctcctcggcg gccatcatca
      301 gccgcccgaa cccggcgacg ggatgccact tgtccgggtc gcccacgagg gcatcgatcg
      361 tgagcccgag agccacggcc cggcctcgtt ttgctagacg gccgacggcc acggcctcat
      421 cgtgcccggc gcgggccggc gcgggccggc ggcggcgaac ccggctgcat ttggcgcgcc
      481 ttcctgggcg cgaaaggtgc cgtttttcac gcctttcggt gccaagaacc ccgggcgatc
      541 agccgggtgc ccaggccgac ccgccatcga ccttgatgac ggcgccggtg gtgtagctcg
      601 acgccccgct ggccaggtag agcgcggcgc ccacgatctc gggaggagca ccggcgcggc
      661 cgagagggat ggtgcgcgcc gccatctgct cgaaggcggg caggtcccac gccttcgaga
      721 tgtcggtgag gaaggggccc ggcatgatgg cgttgaccct gaccgtcggg gcgaaggccc
      781 gggccatccc cagcgtggcg gcgttgagcc cggccttggc caccgcgtag ggcagctcgc
      841 cgacgccggg ctgcacggcg gcgatcgagc tcacgttgac gatggacccg cccccgccct
      901 gggccatgcg ggtgccgacc agcgccatga gccggaaggc gcccttgagg ttgacgtcga
      961 tgaccttgtc gaacaggtcc tcggtcacgg cgtcgagcga cgggtacagg ggcgacatgc
     1021 ccgcgttgtt gaccagcacg tcgatgcggc cgtaggcgtc gacgacctgg cggacgagct
     1081 cgtcgcagtc ctcccatctg cccacgtggc acccgacgcc gagcgccggc cgccccgtct
     1141 tggccgtgat gtcggcggcc agtgagacgc aggcctccac cttccggctg gcgacgacca
     1201 ccgtggcgcc gtggtcggcg aaggccagcg ccatctccct gcccaggcca cggctgccgc
     1261 cggtgatgac ggcgacgcgg tccgacaggt cgaacgtgag gtccggggag gggggcacgg
     1321 ccgtagcttt agcacccggg cggcgcagcc gtcagttggc cagccgttcc tcgacggcgt
     1381 ggaccttggc gtgcaaggcg tccgcgacac cggcgcggtg gtcgaccttg atgaccaggc
     1441 tgacccgggg cgcagaggcg gccacctcgt ccacgcaccg tttgatcacg cccatgacct
     1501 cgtcccagtc gccctcgacg ttggtgaaca tggcgttggt ctcgttgggg agcccgctgg
     1561 cgcgcacgat gccgacgcag gtggcgacca ggtcgccgac cgactcgccg gcg
//
LOCUS       DASWGG010000448         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2524146, whole genome shotgun sequence.
ACCESSION   DASWGG010000448 DASWGG010000000
VERSION     DASWGG010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2524146"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..89)
                     /locus_tag="VF954_08200"
     tRNA            complement(16..89)
                     /locus_tag="VF954_08200"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Gln,seq:ttg)
     gene            165..263
                     /locus_tag="VF954_08205"
     CDS             165..263
                     /locus_tag="VF954_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEX9531105.1"
                     /translation="MGSLIKKRRKRMRKKKHKKMLKATRWQRRAGK"
     gene            complement(299..685)
                     /locus_tag="VF954_08210"
     CDS             complement(299..685)
                     /locus_tag="VF954_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9531106.1"
                     /translation="MTEMTELAERFCKGIEAGDIEVVKECYSPDVVVWHNFDRRDQSR
                     DENLASLAWFLDRVEALRYEEVRRVVHAEGFVQQHILRGRTPAGVELEAPVCLVAEVA
                     GGRITRLEEYLDSAQLAALAAAGPGA"
     gene            complement(708..1436)
                     /locus_tag="VF954_08215"
     CDS             complement(708..1436)
                     /locus_tag="VF954_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX9531107.1"
                     /translation="MPDVVRALAKLTLSLRVTGRRRDGYHLIDAEMVTIDLADTLELG
                     PGHGLEIVDERAPAAAAEGPDLLALGDGNLISRALVAVQRQAHVRLVKRIPVGAGLGG
                     GSADAAAVLRWAGADDLALAARLGADVPFCLVGGRARVTGAGEVVEPLDPRPQTFTVL
                     SPPLHVPTLEVYRAWDELGGPAGAGPNDLEPAALAVEPRLARWRDRLADATGRVPALA
                     GSGGTWFVEGAFPAAGVVVQTAQP"
     gene            complement(1436..>2306)
                     /gene="rsmA"
                     /locus_tag="VF954_08220"
     CDS             complement(1436..>2306)
                     /gene="rsmA"
                     /locus_tag="VF954_08220"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX9531108.1"
                     /translation="VPGGDTGQDHVVTLTRPQISDLLSRHRLRPSRALGQNFVADPNT
                     VRRVARLAAAGPGDRVVEIGAGLGSLTLALAETGASVLAVEIDRHLVPALRSVVEPAG
                     VEVLEGDALALDWPAVLAERHAGPWALVANLPYNIATPLVIRLLEEVPAIARMLVMVQ
                     REVAERLAAPAGTEPYGAASVKVAYWATAAVVGRVPPTVFVPRPRVDSALVSIVRRPV
                     PGVAGAVVGYERLFAVVRAGFAQRRKMLRRALEGVVSPAAFAAAGVAPTARAEELDLE
                     AWARLAAATAAPG"
BASE COUNT          329 a          901 c          823 g          253 t
ORIGIN      
        1 gctccggagg aggggctccg gggggagggc tcgaacctcc aagcatccgg ctccaaaggc
       61 cggcgttcta ccgattgaac taccccggac cgaccgccca acggtactcg tgcccgagat
      121 gccaccgcgg ccgccgcggg ccggccggta gcgtgacggc cgccatggga tcgctgatca
      181 agaagcgccg caagcgcatg cggaagaaga agcacaagaa gatgctgaag gcgacccgct
      241 ggcagcgccg ggccgggaag taggccccgg gcgcggcggt gcgagggagg tggccgggtc
      301 aggcgccggg cccggcggcg gccagggccg ccagctgcgc cgagtccagg tactcctcca
      361 gccgggtgat ccggccgccg gccacctcgg cgaccaggca gacgggcgcc tccagctcga
      421 ccccggccgg cgtgcggccg cgcaggatgt gctgctggac gaagccctcc gcgtgcacga
      481 cccggcgcac ctcctcgtac cgcagggcct cgacgcggtc gaggaaccac gccagcgagg
      541 cgaggttctc gtctcgtgac tggtcccggc ggtcgaagtt gtgccacacc accacatcgg
      601 gcgagtagca ctccttgacc acctcgatgt cgcccgcctc gatccccttg cagaagcgct
      661 cggccagctc ggtcatctca gtcatggcag ccatcccgtg gtcccggtca tggctgtgca
      721 gtctgcacca ccacgccggc ggctggaaag gcgccttcga cgaaccacgt ccccccgctg
      781 ccggccagcg ctggcacccg acccgtggcg tcggccagcc ggtcccgcca acgggccagc
      841 cggggctcga cggcgagagc cgcgggctcc agatcgttgg ggcccgcgcc cgccggcccc
      901 cccagctcgt cccacgcccg gtacacctcc agggtcggga cgtgcagcgg cggtgacagc
      961 accgtgaagg tctgcggccg ggggtcgagg ggctcgacca cctcaccggc gcccgtcacc
     1021 ctcgcccggc cgcccacgag gcagaagggc acgtccgccc cgagccgggc ggccagcgcc
     1081 aggtcgtcgg ccccggccca ccgcagcacc gcggcggcgt cggccgagcc gcccccgagc
     1141 ccggcgccca ccgggatccg cttgaccagc cggacgtggg cctggcgctg cacggcgact
     1201 agcgcccggc tgatcaggtt gccgtcaccc agggcgagca ggtcggggcc ctcggccgcc
     1261 gccgccgggg cgcgctcgtc gacgatctcc agcccgtggc cgggcccgag ctccagcgtg
     1321 tcggccaggt cgatggtcac catctcggcg tcgatcaggt ggtagccgtc ccgtcgccgg
     1381 cccgtgacgc gcaacgagag ggtcagcttg gccagggcgc gcaccacgtc gggcatcaac
     1441 ccggggcggc ggtggccgcg gcgagacggg cccatgcttc gagatctagc tcctcggccc
     1501 tggccgtggg ggccacgccc gcggccgcga acgcggccgg cgacaccacc ccctcgagcg
     1561 cccgccgcag catcttgcgg cgctgggcga agccggcccg caccacggcg aacaaccgct
     1621 cgtagcccac caccgccccg gccaccccgg gcacggggcg ccggacgatc gagacgagcg
     1681 ccgagtccac ccgcggccgg ggcacgaaca ctgtcggtgg gacccggccc acgacggccg
     1741 ccgtcgccca gtaggccacc ttcaccgacg ccgccccgta cggctcggtc ccggccgggg
     1801 cggccagccg ctcggccacc tcgcgctgga ccatgaccag catcctggcg atggcgggca
     1861 cctcctcgag gagccggatc accagcgggg tggcgatgtt gtagggaagg ttggcgacca
     1921 gcgcccacgg cccggcatgg cgttcggcga gcaccgccgg ccagtccagc gcgagggcgt
     1981 cgccctcgag gacctccacg ccggcgggct cgacgaccga gcgcagcgcc ggcaccaggt
     2041 gccgatcgat ctccacggcg aggaccgacg ctcccgtctc ggccagggcc agggtcaggc
     2101 tcccgaggcc ggccccgatc tcgaccaccc gatcccccgg cccggcggcc gccaaccggg
     2161 ccacgcggcg cactgtgttg ggatcggcca cgaagttctg gcccagcgcc cggctggggc
     2221 gaaggcggtg gcgactcagc aggtcggaga tctgggggcg cgtcagcgtc accacgtgat
     2281 cttgacctgt atcacccccc gggacg
//
LOCUS       DASWGG010000449         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2532608, whole genome shotgun sequence.
ACCESSION   DASWGG010000449 DASWGG010000000
VERSION     DASWGG010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2532608"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VF954_08225"
     CDS             complement(<1..1087)
                     /locus_tag="VF954_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531109.1"
                     /translation="MSRARYRCALGIWIPQFAPAVESDLARLRALADGDGPGAHGLLL
                     ALAFRAVFKGEPREEIIGMVERGLSGIEAEAGHPTSPVAWAVRSLTFLDELDRAEAVL
                     ADMFAHAQRTGSAPTFAAASVCRAALALRRGMIEAAEEDARRALELVATHQIAFLSPH
                     AHTHLGEALLERGELDEAAALLEGADLAAMRGSRPEALFLYTRGRARLARGDVAVGVA
                     DLRSCLSIQESVGFANPNVMACRSTLALSLPTSQRHEARSLVQVELDQARAIGQPRAI
                     GVALRAQALLSGHDQAIPLLRDAVATLVGSPSRLEHSRALTDLGAALRRANQRADARE
                     PLRQALDHASRSGATALAGRARDELVAT"
     gene            1341..1535
                     /locus_tag="VF954_08230"
     CDS             1341..1535
                     /locus_tag="VF954_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531110.1"
                     /translation="MELRLELTADAQTVRGSVRDQDGHQRSFWGWLELMEALQQLGGP
                     QDHTDPTPDSVRDPEGRLRT"
     gene            1679..2554
                     /locus_tag="VF954_08235"
     CDS             1679..2554
                     /locus_tag="VF954_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9531111.1"
                     /translation="MRRFSFIVGLAAIALAAAACASSSPHTATPAATPTSSTTTPAAS
                     PAVLADLAPTGKLRLAVWVGPEFLAHGNPPAGIAVDLATKLASRLGVPLVTTIYDTPP
                     ALLAAAQSPGWDVAVLPILAATSSAVDFAAPVILIPHTLLVRNGSSISTMAQADQPGV
                     TIASLARGPHTAALASRLKKAKLVPVASDTAGLAMLKTGQVDAFGDGRFAMSEDMAQA
                     PGSHVLTEDFFTGHAGLAVTKGHAAGLAYLTEFAEELKASGAVQQAITAAGVSDVSVA
                     PPGSSVPTPPPTAAP"
     gene            2678..2857
                     /locus_tag="VF954_08240"
     CDS             2678..2857
                     /locus_tag="VF954_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9531112.1"
                     /translation="MPKLAAVVKEQRRKQLLDAAWRCVARGGYRNLSVDDVCAEAGLC
                     SSEPTFGPRSPQMGC"
BASE COUNT          467 a         1158 c         1066 g          426 t
ORIGIN      
        1 cggtggccac cagctcgtcg cgggcccgcc cggcgagggc ggtagcgccg gaccgggagg
       61 cgtggtcgag ggcttggcgc agcggttcac gagcgtccgc gcgctgattg gctcggcgca
      121 gcgccgcgcc gaggtcggtg agggcgcggg agtgctcgag ccgggagggt gaccccacga
      181 gtgtggccac ggcatctcgg agcagaggaa tggcctggtc atggccggag agcagggcct
      241 gggcgcgaag ggccacgccg atggcccggg gctggccgat ggcccgggcc tggtctagct
      301 cgacctggac gagagagcgg gcctcgtggc gctggcttgt cggaagggag agggcgaggg
      361 tggaacggca ggccatcacg ttggggttgg cgaagccgac cgactcctgg atcgacaggc
      421 acgatcgaag gtcggccact cccaccgcca cgtcaccccg ggccaggcga gcccgcccgc
      481 gggtgtacaa gaacagcgct tcggggcgtg agccgcgcat ggccgccagg tcggcgccct
      541 ctagcagcgc ggccgcctcg tcgagctcac cgcgctctag gagggcctcg ccgaggtggg
      601 tgtgggcatg gggggagagg aaggcgattt ggtgggtcgc gaccagctcc agggcacggc
      661 gggcgtcctc ctcggccgcc tcgatcatgc ccctgcggag ggcgagggcc gctcgacaaa
      721 cggaggcggc cgcgaaggtc ggcgccgacc cggttcgctg agcgtgcgcg aacatgtcgg
      781 cgaggacggc ctcggcccgg tcgagctcgt cgaggaaggt caggctgcgc accgcccagg
      841 cgacggggga cgtcgggtgg ccggcctcgg cctcgatccc ggacaggcct cgttcgacca
      901 tcccgatgat ctcctcgcgg ggctcccctt tgaacaccgc ccgaaaggcg agggcgagca
      961 gcaacccgtg ggcgccgggc ccgtcgccgt cggcgagcgc ccgtagcctc gccaggtccg
     1021 actcgacggc gggagcgaac tgtgggatcc agatgccgag ggcgcaccgg taccgcgccc
     1081 tcgacaacca ccagctgacc gttgcctgac gcgctggcgt cgatcagttt accgatctgg
     1141 gcgagatcgg tctcgcgctc caacacctcc tggtgctgcg ccggcacttc ccgccctcga
     1201 tcccgctccc acatcgtgcc aaccgaggat aggaccgcgt ccgtacggac ggctcttgcc
     1261 aaaacttcgg gggggcctac cgatgcgaac cgcacggcca ggcgcccacg atcccaggca
     1321 tcgcctacca atccccaacc gtggagttgc gcctggagct caccgccgac gcccagacgg
     1381 tccgcgggtc cgtccgggac caggacggcc accaacgctc cttctggggg tggctcgagc
     1441 tgatggaggc cctccagcaa ctcggcgggc cgcaagacca cacggacccg acgccagaca
     1501 gcgtgcgcga ccccgagggg cgtctccgga catgaccccc cgaccttgcg cttccgccag
     1561 cttcggggcg cgccgaacgc cccgaagcga aagaggtccc cgctggcgcc ggcctatctg
     1621 ctccgtgcag tcgccccccg gcgacgctcc ccgcccacca accccaagga gaccagccat
     1681 gcgtagattc agcttcatcg tcgggctggc ggcaatcgct cttgccgccg ccgcctgcgc
     1741 cagcagctcg ccccataccg ccacacccgc ggccacgccg acgtcctcca ccacgacacc
     1801 ggccgcttcg ccggcggtcc tcgccgatct ggccccaacc ggcaagctgc gcctggccgt
     1861 ctgggtcggt cccgaattcc tggcccacgg caacccaccg gctggcatcg ccgtcgacct
     1921 ggccaccaag ttggcgtcgc gcctgggcgt gcccctcgtg accacgatct acgacacccc
     1981 gcccgcgctg ctcgccgccg cccagagccc cggctgggac gtggccgtgc tacccatcct
     2041 cgccgccacc tcgagcgcgg tggacttcgc cgcacccgtg atcctgatcc cccacaccct
     2101 gctcgtccgg aacggatcat cgatctcgac catggcccag gccgaccaac ccggcgtcac
     2161 catcgcctcc ctggcccgtg gaccccacac cgccgcgctc gccagccgcc tcaagaaggc
     2221 caagctggtg ccggtggcca gcgacaccgc cggcctcgcc atgctcaaaa ccggccaggt
     2281 cgacgccttc ggcgacgggc gcttcgcgat gtccgaagac atggctcagg cgccgggcag
     2341 ccacgtgctc accgaggact tcttcaccgg ccacgcgggg ctagccgtga ccaaaggcca
     2401 cgccgccggc ctcgcctacc tcaccgagtt tgccgaggag ctcaaagcct ccggggccgt
     2461 ccagcaggcc atcacagcgg ccggtgtgtc cgacgtgtcc gtggcgcccc caggctcctc
     2521 tgtccccaca ccgcctccta ccgcggcgcc ctgagttgtg gtcgaccacc ttgaagtccc
     2581 gggcgggcgc cgaggccgtc catcgagcgg cccgtagctc atatcgaacg tcatggaaaa
     2641 ctgactagtc ggttgtgatt ccattactct ggttgccatg ccgaagttgg ccgccgtggt
     2701 caaggagcag cgtcgcaagc agttgctgga cgcggcctgg cggtgcgtgg cccgcggtgg
     2761 ctaccgcaac ctcagcgtcg acgacgtgtg cgccgaggcc ggcctgtgca gctccgagcc
     2821 gacctttggg ccgagatctc cccagatggg atgctgaggg cacgtttcct ggaggcgatg
     2881 caacagcgac gggtccgcct ggctgagctc atcgagaacg cggtcgacgc gctgctcgaa
     2941 gggctgcgcg ccgagcctcg aagctgacgg tggccgccac cgaaccggcc aagaggaagc
     3001 gccggtcgcc tgcctggcgc gaccccggtg tcgcgtgggc gcggctcgcc cgacaccgcg
     3061 acaacgtgtt cgccctgggg cgccgcaccc gccaagtggt gttgttggcg gcgctga
//
LOCUS       DASWGG010000450         3615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2538868, whole genome shotgun sequence.
ACCESSION   DASWGG010000450 DASWGG010000000
VERSION     DASWGG010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2538868"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VF954_08245"
     CDS             complement(<1..218)
                     /locus_tag="VF954_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531113.1"
                     /translation="MSLQNVRVVATGLEFPEGPIAMADGSVVLVEIKRGTVSRARPDG
                     TVEVVARLGGGPNGAAIGPDGAVYVCNNG"
     gene            complement(233..583)
                     /gene="queF"
                     /locus_tag="VF954_08250"
     CDS             complement(233..583)
                     /gene="queF"
                     /locus_tag="VF954_08250"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX9531114.1"
                     /translation="MPSSPSRELVGLPNPHPGRDYEIRCETPEFTCVCPMTGQPDFAT
                     VAISYVPGASIVELKSLKLYLWSFRQEGVFHEDVTNRILDDLVGVLAPRRMTVTTDWL
                     VRGGIHTVVQAAHP"
     gene            complement(631..783)
                     /locus_tag="VF954_08255"
     CDS             complement(631..783)
                     /locus_tag="VF954_08255"
                     /inference="COORDINATES: protein motif:HMM:NF039193.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX9531115.1"
                     /translation="MATYEGYCVKCREKRQFDGQQVELANGRKAAQGTCPVCGTKMNR
                     MLGKTG"
     gene            complement(863..1747)
                     /locus_tag="VF954_08260"
     CDS             complement(863..1747)
                     /locus_tag="VF954_08260"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX9531116.1"
                     /translation="MLARAPASSANLGPGFDTLAVALGLYTEVEVEPAGSLHVHAEGE
                     GSDLPADAGHLAARVATSVVGHDRLHITVRSQVPVSRGLGSSAALAVAAAAAAGADDP
                     LAVAAAFEGHPENAAASVLGGLVTATFVGSSPVAAPLTLDPAITYVVLVPGRPLATER
                     ARAALPAHVPHTDAAFNLGRMGLLIAGLGDHRQLVREATDDRLHQTARGPLFPEAAFL
                     LAGLLDAGALASCWSGAGPSLLAFCLAESVPTLVTAGRRLLQQAGIGGQAIAVRPDLE
                     GLEVGAQPRVEAVGAVPR"
     gene            1851..2180
                     /locus_tag="VF954_08265"
     CDS             1851..2180
                     /locus_tag="VF954_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531117.1"
                     /translation="MYTFAIVVLLGLALFKVVDLLEDLVPALTRFHTLVTLALAVAGT
                     VALDYSLFSGFHVVLRNSWMGSWTTGLIVAGTTSAWRAAFHWLGSSEGEEPEVRHTSE
                     HHPRIAA"
     gene            complement(2249..>3615)
                     /locus_tag="VF954_08270"
     CDS             complement(2249..>3615)
                     /locus_tag="VF954_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX9531118.1"
                     /translation="LAAHGVATKLVGMTVGPTVTRYELELGAGVKVARVTSLHRDIAY
                     AMASPDVRILAPIPGRSAIGVEVPNRQRELVALADILASGEAHRAKQPLEVALGRDIA
                     GRPVIVNLADMPHILIAGATGAGKSSCINSTITSILMRSTPEQVRMILVDPKRVELGQ
                     YNGLPHLLTQVVVNPKKAANALAWAVHEMERRYDLLAEVGVRDITGYNAAFDRGELGA
                     GSDAEREFARLPFILVVVDELNDLMMVAARDVEDAICRIAQMARAVGIHLVIATQRPS
                     VDVITGVIKANIPSRLAFSVSSLADSRVILDQPGAERLIGKGDLLLLTASSSVPRRIQ
                     GPWVSEAEVRAVVAHWRRQAQPDYVAGVEGDDGPGGGFGLASGEDDDDGLLEQALEMV
                     VRSQLGSTSMLQRKLRVGFARAGRLMDLLERRGVVGPSEGSKAREVLMTPEELDARQQ
                     GAAG"
BASE COUNT          502 a         1328 c         1275 g          510 t
ORIGIN      
        1 ccgttgttgc agacgtagac ggcgccgtcc ggcccgatgg cggcgccgtt gggcccgccg
       61 ccgaggcggg cgacgacctc cacggtcccg tccggccggg ccctcgacac ggtgcctcgc
      121 ttgatctcca ccagcacgac gctgccgtcg gccatggcga tgggcccttc ggggaactcg
      181 agtccggtgg cgacgacacg cacgttttgg aggctcacgg ggccccatcc catcacggat
      241 gcgcagcctg caccaccgtg tggatcccgc cccgcaccag ccagtcggtc gtgacggtca
      301 tgcggcgcgg cgcgagcacc ccgaccaggt cgtcgaggat gcggttggtg acgtcctcgt
      361 ggaagacgcc ctcctggcga aagctccaca ggtacagctt caggctcttg agctcgacga
      421 tcgacgcgcc cggcacgtag ctgatggcca cggtggcgaa gtcgggttgc cccgtcatcg
      481 ggcagacgca ggtgaactcg ggcgtctcgc accggatctc gtagtcccgg cccgggtgcg
      541 ggttgggcag cccaaccagt tcccgggacg gcgagctcgg cacgcctgaa acgctatcgc
      601 tcggtgcccg ccatcaggcg gggccggcga tcagccggtc tttccgagca tgcggttcat
      661 cttcgtccca cagacggggc aggtgccttg ggccgccttg cggccgttgg ccagctccac
      721 ctgttgaccg tcgaactgac gcttctcccg acacttcacg cagtagccct cgtaagtcgc
      781 catcggtttg gacctccttg ctggcggcga gacgacgcta gccgccctgt gcccgcgggc
      841 cgcggatggt cccaaaccgc gttcagcgag ggacggcgcc gaccgcctcc acgcggggct
      901 gggctcccac ctcgaggcct tccaggtcgg gccgcacggc gatcgcctga ccgccgatgc
      961 ccgcctgttg cagcagccgc ctgcccgcgg tgacgagcgt gggcaccgac tcggccaggc
     1021 agaaggcgag caggctgggc ccggcgccgg accagcagct cgccagggcc ccggcgtcga
     1081 gcaggccggc gaggaggaac gcggcctccg ggaacagcgg gccgcgcgcc gtctggtgca
     1141 ggcggtcgtc ggtcgcttcc cgtaccagct ggcggtgatc gcccagcccc gcgatcagca
     1201 ggcccatccg gccgaggttg aacgccgcgt cggtgtgggg gacgtgagcc gggagggcgg
     1261 cccgggccct ttccgtggcc agcggccgcc ccgggacgag gaccacgtac gtgatcgccg
     1321 ggtccagggt cagcggcgcg gccaccgggc tggaaccgac gaaggtggcg gtcaccagcc
     1381 cgccgagcac cgacgcggcc gcgttctccg gatggccctc gaaggcggcc gccaccgcga
     1441 gcgggtcgtc cgccccggcg gccgcagccg ccgccaccgc cagcgccgcg gacgacccca
     1501 ggcccctgct caccgggacc tgggaccgca cggtgatgtg gagccggtcg tgccccacga
     1561 ccgaggtcgc cacccgggcg gccagatggc cggcgtcggc gggcaggtct gaaccctcgc
     1621 cttcggcgtg gacgtgcaac gagcccgccg gctccacctc gacctcggtg tagaggccca
     1681 gggcgaccgc gagggtgtcg aagccagggc ccaggttggc cgacgacgca ggcgcccggg
     1741 cgagcatgac cgccagatta ccggccgggc gtttgtcacc ttcgaagcgg cccgcgggtc
     1801 gaagttgatg acgggacacc cacgaccggt cccgtcagga ggcagtgaac atgtacacct
     1861 tcgccatcgt tgtcctgctt ggactggcgt tgttcaaagt cgtcgacctg ctcgaggacc
     1921 tcgtgccggc tctcacccgt ttccacacgc tcgtcaccct ggccctcgcc gtggccggga
     1981 cggtagccct ggactactcc ctgttctcgg gcttccacgt cgtgctgcgc aactcgtgga
     2041 tgggctcctg gacgacaggg ctcatcgtcg cgggcacgac gagcgcgtgg cgggcggcct
     2101 tccactggct cggctcgtcc gagggtgagg agcccgaggt gcgccacacc tccgagcacc
     2161 acccgcgcat agccgcctga gcggcaccag caccagcctc accgtcgacg cgaggggcgc
     2221 ccccaggggc gcccctcgtc gcgcccggtc agcccgcggc accctgctgg cgggcgtcca
     2281 gctcctcggg ggtcatgagg acctcgcggg ccttggaccc ttcggacggc cccaccacgc
     2341 cccgccgctc gagcaggtcc atgagccgcc cggcccgggc aaagcccacc cggagcttgc
     2401 gctggagcat cgacgtcgac cccagctgcg accggaccac catctccaga gcctgctcga
     2461 gcaggccgtc gtcgtcatct tcgccggacg ccaggccgaa gcccccgccc gggccgtcgt
     2521 cgccctcgac gcccgccacg tagtccggct gggcctgacg gcgccagtgg gcgacaaccg
     2581 cccggacctc ggcctcgctg acccacgggc cctggatgcg acggggcacg ctggacgagg
     2641 cggtcagcag caggaggtcg cccttcccga tgagccgttc ggcgccaggc tggtcgagga
     2701 tgacccggct gtcggccagg gacgacaccg agaacgccag gcgggacggg atgttggcct
     2761 tgatgactcc ggtgatgacg tcgaccgacg gccgctgggt ggcgatgacg aggtggatgc
     2821 cgacggctcg cgccatctgg gcgatccggc agatggcgtc ctccacgtcg cgggccgcca
     2881 ccatcatcag gtcgttgagc tcgtcgacga ccaccaggat gaacggcagc cgggcgaact
     2941 cccgctccgc gtccgacccg gcccccagct cgccccggtc gaacgccgcg ttgtagccgg
     3001 tgatgtcgcg cacgccgacc tcggccagca ggtcgtagcg cctctccatc tcgtgcacgg
     3061 cccatgccag cgcgttggcc gccttcttgg ggttgaccac gacctgggtg agcaggtggg
     3121 gcaggccgtt gtactggccg agctcgaccc gcttggggtc gaccaggatc atgcggacct
     3181 gttcgggcgt cgagcgcatc aggatcgacg tgatggtcga gttgatgcac gacgacttgc
     3241 ccgcgcccgt cgccccggcg atcaggatgt gaggcatgtc ggccaggttg acgatcaccg
     3301 gccggccggc gatgtcgcgg cccagggcga cctccagcgg ctgcttggcc cggtgggcct
     3361 ccccgctcgc caggatgtcg gcgagcgcca ccagctcccg ctgccggttg ggcacctcca
     3421 cgccgatggc cgagcggccc gggatcgggg cgaggatgcg cacgtcgggc gaggccatgg
     3481 cgtaggcgat gtccctgtgg aggctggtca cccgggccac cttgacaccg gcgcccagct
     3541 cgagctcgta gcgggtcacg gtgggaccca cagtcattcc gaccagcttg gtcgccaccc
     3601 cgtgggcggc caggg
//
LOCUS       DASWGG010000451         3861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2540416, whole genome shotgun sequence.
ACCESSION   DASWGG010000451 DASWGG010000000
VERSION     DASWGG010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540416"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VF954_08275"
     CDS             <1..749
                     /locus_tag="VF954_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531119.1"
                     /translation="LAPPLDDGAPYGRLGLAWFAITVPVLVAGPRWLAAWLALHAAVA
                     GAQMAMSWRRAGPRVVAPAAALGAVIATAGFVAGTAVGIAGLVGGALLSAAAAGLVRA
                     GRGRSPARGPAVVCALVPGLAAGSLLAARTSGAAAGLALLLVVCTWDGANYIMGTEAR
                     WRWTGPAAAALAVGVVTLAAAAAGSPPVTGATAWVLGGAAVLLTPIGPRLATLACGDR
                     TANLSALRRLDGLLLTGPAWWVLVHVLPGR"
     gene            complement(787..1206)
                     /gene="sodN"
                     /locus_tag="VF954_08280"
     CDS             complement(787..1206)
                     /gene="sodN"
                     /locus_tag="VF954_08280"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEX9531120.1"
                     /translation="MRIVPRLLAVADRISAPLPASAHCDLPCGIYDPAQARLEAEAVK
                     ACMEKYAESDDADFRVRAVLVKEERAELVKHHLWVLWTDYFKPGHVDRYPQLHDLFWK
                     ATKAAGEAKKTQDVAIAQGLLDQIAEIDRIFWETKKD"
     gene            1334..1660
                     /gene="sodX"
                     /locus_tag="VF954_08285"
     CDS             1334..1660
                     /gene="sodX"
                     /locus_tag="VF954_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-type superoxide dismutase maturation
                     protease"
                     /protein_id="HEX9531121.1"
                     /translation="MEVRGDSMRPTLLPGDRLAVVRGLPVRAGDVVALADPRRPDRIM
                     VKRAEAETPGGWFVVGDEPGASTDSRHFGPVPAGLVIGRAVYRYAPDGRVGRLRRGAG
                     RPPPTG"
     gene            1674..2207
                     /locus_tag="VF954_08290"
     CDS             1674..2207
                     /locus_tag="VF954_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531122.1"
                     /translation="MAIARIEELLNPAFVADLGGLSLSDVRARRDQCQVAADTLSYLR
                     RLVQGRLDIVHADLERRAGGEQGDLADLVDSLPEILSDGIRGAGLGRLPTSFSPADAD
                     GWISEELDALADADRLGRLPELDEPAVRELANHLYELERKVSDQRHRLFDAANALQEE
                     IVRRYKSGEANVDTLLR"
     gene            2204..2701
                     /locus_tag="VF954_08295"
     CDS             2204..2701
                     /locus_tag="VF954_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9531123.1"
                     /translation="MTGPEDPAGPNGRRAAGGPPSPAWPGLPALPVVRELPDRWRELG
                     EFIREQRGVSRLSLRRLSELAGISNPYLSQIERGLRKPSAEILQQIARGLRISAETLY
                     IRAGILEERDEGEDLVGAILRDRTLTEDQKQALIKVYQSFRHENEDRPVSPRIDDAAG
                     GTAGR"
     gene            2732..>3861
                     /locus_tag="VF954_08300"
     CDS             2732..>3861
                     /locus_tag="VF954_08300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9531124.1"
                     /translation="MRRLAVLSMHTSPLAQPGTGDGGGMNVYVRELSAGLARAGVACD
                     VYTRAESAEAAAIVAVEPGLRVHHIPAGALGPVPKQSLPGLVDEFADGVLARMAAQRS
                     WGEDPAELIHANYWLSGVAGHRMKHELGLPLVSTFHTLARVKAESSPEELSAHEPARR
                     TAAEVAVIGCSDAILASCDVEALQIAELYHADPDRIELVPPGVDHAFFAPGNRAQARR
                     ALGLPAERRMLLFVGRIQPLKGLDVAIGALAALSDHLDAFLVVVGGPSGPQGEHEVAR
                     MHALARDRGVAGRVRFLPPQRHELLSTFYRAADVALVPSRSESFGLVALEASACGTPV
                     VATAVGGLRTLVEDGATGFLVEDRDPFVFAKAVAAVLGDRDLA"
BASE COUNT          432 a         1395 c         1472 g          562 t
ORIGIN      
        1 gcctggcccc gcccctcgat gacggcgctc cgtacgggcg tctcggcctg gcgtggttcg
       61 ccatcacagt ccctgtgctc gttgccggcc cgcggtggct ggcggcctgg ctggcgctgc
      121 acgccgccgt ggcgggcgcg cagatggcca tgtcgtggcg gcgggccggg ccgcgggtgg
      181 tggcgcccgc ggccgcgttg ggggcggtga tcgccaccgc cgggttcgtg gcgggcaccg
      241 ccgtgggcat cgctggactg gtcggcggtg cgctgttgtc cgccgcggca gccggcctgg
      301 tgcgagcggg gcggggccgg tcgccggccc gggggccggc ggtggtctgc gcgctcgttc
      361 ccggcctggc cgcgggctcg ctcctcgcgg cccgcacctc cggagcggcg gccgggctgg
      421 cgctgctcct ggtggtctgc acgtgggacg gcgccaacta cataatgggg acggaggccc
      481 ggtggcgctg gaccgggccc gccgccgccg ccctggcggt gggggttgtg acgctcgccg
      541 ccgcggccgc cggctcgccg cctgtcaccg gcgccacggc gtgggtgctg ggcggcgcag
      601 cggtgttgct gacccccatc ggcccgaggc tggcgacgct ggcgtgcggg gaccgcacgg
      661 ccaacctgtc ggcgctgcgg cggctcgacg gcctgctgct caccggcccg gcgtggtggg
      721 tgttggtcca tgtcctccca gggcgctgac caatgtggcg cgatggcggg gtggtgccgg
      781 tccgggtcag tccttcttgg tctcccagaa gatgcggtcg atctcggcga tctggtccag
      841 cagcccctgg gcgatcgcca cgtcttgggt cttcttggcc tcgcccgcgg ccttggtcgc
      901 cttccagaac aggtcgtgaa gctgggggta ccggtccacg tgaccgggct tgaagtagtc
      961 ggtccacagg acccacaagt ggtgcttcac cagctcggcc ctctcctcct tgaccagcac
     1021 ggcccggacc cggaagtcag cgtcgtcgga ctcggcgtac ttctccatgc acgccttgac
     1081 cgcctcggcc tcgagccgcg cctgcgcggg gtcgtagatg ccgcagggca ggtcgcagtg
     1141 agcggaggcc gggaggggcg cggagatgcg gtcggcgacg gccaagaggc gggggacgat
     1201 gcgcatgcgt gactccttgc gtggggggcg tcgggctggt ggtcacccta ccggcgggtc
     1261 gtgggcgcgc ctcatgtggc tggcctcggt cggcgccggt atcggggcgc tggcctgggg
     1321 ccgagttcgc cgggtggagg tccgcggcga cagcatgcgg cccaccctcc tgcccggcga
     1381 ccgcctggcc gtggtccgcg gcctgccggt gagggcgggc gacgtggtgg ccctggccga
     1441 cccccgtcga cccgaccgga tcatggtcaa gcgggccgag gccgagacgc ccggcggttg
     1501 gttcgtcgtg ggcgacgagc cgggcgcgag caccgacagc cgccatttcg gcccggtccc
     1561 ggcgggcctg gtcatcggcc gggcggtcta ccggtacgcg cccgacggac gggtcggccg
     1621 gctccgcaga ggggccggcc ggccaccgcc gaccgggtaa catcggcgtt gtcatggcga
     1681 tcgcccgcat cgaggagctg ctcaacccgg cgttcgtggc cgacctgggc gggctgtccc
     1741 tgtccgatgt ccgggctcgc cgagaccagt gccaggtggc cgccgacacc ctgtcgtacc
     1801 tgcgccgcct ggtgcagggc cgactcgaca tcgtgcacgc cgacctagag cgccgggccg
     1861 gtggggagca gggcgacctc gccgacctcg tcgacagcct gcccgagatc ctgtccgacg
     1921 gcatccgcgg cgccggcctg ggacggctcc ccacctcctt ctcgccggcc gacgcggacg
     1981 gctggatcag cgaggagctc gacgccctcg ccgacgccga ccgcctgggc cggctcccgg
     2041 agctcgacga gccggccgtc cgcgagttgg ccaaccacct gtacgagctc gagcgcaagg
     2101 tctccgacca gcgccaccgt ctcttcgacg cggccaacgc cctccaggag gagatcgtgc
     2161 gccggtacaa gagcggcgag gcgaacgtcg acaccctgct ccggtgaccg gccccgagga
     2221 tcctgcgggg ccgaacggcc gccgggcggc cggcggcccc ccgtcgcccg cgtggcccgg
     2281 cctgccggcc ttgccggtcg tgcgggagct tcccgaccgc tggcgcgagc tgggtgagtt
     2341 catccgcgag cagcggggcg tgtcccggct gtccctgcgc cggctgtcgg agctggccgg
     2401 gatctccaac ccctacctga gccagatcga gcgcgggctt cgcaagccct cggccgagat
     2461 cctccagcag atcgcccggg gcctccgcat ctcggccgag acgctctaca tccgggcggg
     2521 catcctcgag gagcgggacg agggcgagga cctcgtgggc gccatcctgc gggaccgcac
     2581 cctcaccgag gaccagaagc aggcgctcat aaaggtgtac cagtccttcc gccacgagaa
     2641 cgaggaccgt ccggtctcgc cgaggatcga cgacgccgcg gggggcaccg ccgggagata
     2701 aatgcgacca gtcgggtagg gtttccccac gatgcggcgt ctggcggtcc tctccatgca
     2761 cacctcgccc ctcgcccagc cggggacggg ggacggcggc ggcatgaacg tctacgtccg
     2821 cgagctctcg gccggcctgg cccgggccgg cgtggcgtgc gacgtgtaca cccgggcgga
     2881 gagcgccgag gccgccgcca tcgtcgccgt ggagccgggg ctccgggtcc accacatccc
     2941 ggccggcgcg ctcgggcccg tgccgaagca gtctctgccg gggctggtcg acgagttcgc
     3001 cgacggagtc ctggcccgca tggcggcgca gcgatcctgg ggcgaggacc cggccgagct
     3061 gatccacgcc aactactggc tgtcgggtgt ggccggccac cgcatgaagc acgagctggg
     3121 gttgccgctg gtgtcgacgt tccacacctt ggcccgggtg aaggccgagt ccagccccga
     3181 ggagctaagc gcccacgagc cggcccgccg caccgcggcc gaggtggcgg tcatcggctg
     3241 ctccgacgcc atcctggcca gctgcgatgt ggaggccctc cagatcgccg agctgtacca
     3301 cgccgatccc gatcgcatcg agctggtccc gccgggggtg gaccacgcct tcttcgcgcc
     3361 ggggaaccgg gcccaggcgc ggcgtgccct cggcctgccg gccgagaggc ggatgctgct
     3421 cttcgtgggt cgcatccaac cgttgaaggg gctcgacgtg gcgatcggcg cgctggccgc
     3481 cctttctgac catctcgacg ccttcctcgt ggtcgtgggc ggtccgagcg ggccgcaggg
     3541 cgagcacgag gtggcccgga tgcatgccct ggcccgggac cgcggcgtgg ccggccgggt
     3601 ccggttcctg ccgcctcagc gccacgagct gctgtccacc ttctaccggg cggccgacgt
     3661 ggccctggtg cccagccgct cggagtcctt cggcttggtc gccctggagg cgtcggcatg
     3721 cggcacgcct gtggtcgcca ccgcggtcgg cgggctgcgg accctggtcg aggacggtgc
     3781 gacgggcttc ctggtcgagg accgcgaccc gttcgtgttt gccaaggccg tcgccgcggt
     3841 gctcggggac cgggatcttg c
//
LOCUS       DASWGG010000452         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2542190, whole genome shotgun sequence.
ACCESSION   DASWGG010000452 DASWGG010000000
VERSION     DASWGG010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2542190"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..701)
                     /locus_tag="VF954_08305"
     CDS             complement(18..701)
                     /locus_tag="VF954_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9531125.1"
                     /translation="MSGKVLVVDDEPEIRRALRTGLGYNDLDVRAAGSGEEALEVAAA
                     WRPDVVLLDLGLPGMDGFEVLRRLRERGSPAAIIVVSVMPGERDKVRALDLGADDYVT
                     KPFGVAELLARIRAVLRRQPAITAGEPVIRAGDLEIDLGRRQVTAGGRLVHLTPTEFD
                     LLRELAVNAGRPVTHTSLLRKVWGEHAIGDRYNTRYVVAQIRRKLGDDPARPRYIVNE
                     PGVGYRLEA"
     gene            complement(698..2179)
                     /locus_tag="VF954_08310"
     CDS             complement(698..2179)
                     /locus_tag="VF954_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9531126.1"
                     /translation="MRAAAGRAVPYLLGAAGVALISAGIGLLRPWVDIPNLGVLYLLL
                     VIWLGARHRLLPALATAILAVLTYEFFFVPPYGTLLISAPHDLLDLLVLLLAAVAGGR
                     LVSGLAEARVQAESLALESGTLYQVAVEALRGQDVAAALQLLCDRAAETPGVLEFTVL
                     AGDPTGLTRLAGREPARDPLKDARWAAEHGSAVGALVRDGRLELVRPVGAEEASAAVP
                     LAGGVALIRYQPGRVDARGEHALLALVSLGGLLLDRRRALAETERASALELSDRLKAA
                     VLSSLSHELKSPLATIRAGLTTLGMKEAGLDQEQRSMVEGLDLQARRLDRLVRDLLTM
                     SRLEAGVTGERAPEDVAELVGAVLQAMRVRLEPYRLEVEIPELPPILGDELQLDEVLT
                     NLLENAAEWTAPGGRIAIGARRRGDMVETWVENQGPAIPPVELRAIFDTFWSGRSGGT
                     GLGLAICRRIVEAHGGTITAVNGRGGPRFTFALPVAAAVAPLR"
BASE COUNT          313 a          840 c          816 g          282 t
ORIGIN      
        1 ggggaggaag cccatcgcta cgcttcgagg cggtagccca cgccgggttc gttgactatg
       61 tagcggggcc gggccgggtc gtcgcccagc ttgcggcgga tctgggccac cacgtagcgc
      121 gtgttgtagc ggtcgccgat cgcgtgctcg ccccagacct tgcgcagcag cgaggtgtgg
      181 gtgaccggac ggccggcgtt gaccgccagc tccctcagga ggtcgaactc cgtcggagtg
      241 aggtgcacga ggcgtccgcc ggcggtgacc tggcgacgcc cgagatcgat ctccaggtcg
      301 cccgcccgga tcacgggctc gccggctgtg atcgcgggct gcctgcggag cacggctcgg
      361 atccgggcca gcagctcggc cacgccgaac ggcttggtga cgtagtcgtc ggcgcccagg
      421 tcgagggcgc gcaccttgtc gcgctccccc ggcatcacgc tcacgacgat gatggccgcc
      481 gggctgcccc gctcgcgcag ccggcgcagc acctcgaagc cgtccatccc gggcaatccc
      541 aggtcgagca gcaccacgtc cgggcgccac gccgccgcca cctcgagcgc ctcctcgccg
      601 ctgccggcgg cgcgcacgtc gaggtcgttg tagcccagcc cggtgcgcag cgcgcgccgg
      661 atctcgggct cgtcgtcgac gaccaggacc ttgccgctca ccgcaggggg gcgacggccg
      721 ccgccacggg cagggcgaag gtgaagcggg gaccgccgcg cccgtttacc gccgtgatgg
      781 ttcccccgtg cgcctccacg atccgccggc agatcgccag gcccagcccg gtgccgccgc
      841 tgcgcccgga ccagaaagta tcgaagatcg cgcgcagctc gaccggcggg atggccggcc
      901 cctggttctc gacccaggtc tcgaccatgt ccccgcggcg ccgggcgccg atcgcgatcc
      961 ggcccccggg cgcggtccac tcggcggcgt tctcgagcaa gttggtgagc acctcgtcca
     1021 gctgcagctc gtcgcccagg atcggcggca gctcgggtat ctcgacctcc aggcggtagg
     1081 gctcgaggcg gactcgcatc gcctgcagca cggcgcccac cagctccgcc acgtcctcgg
     1141 gcgcccgctc gccagtgacg ccggcctcca ggcgggacat ggtcagcagg tcgcggacca
     1201 ggcggtcgag gcgccgggcc tggaggtcca ggccctcgac catcgagcgc tgctcctggt
     1261 cgaggccggc ctccttcatg cccagggtcg tgaggccggc ccggatcgtg gccagcgggc
     1321 ttttgagctc gtgcgagagg gaggagagga cggccgcctt gagccggtcc gagagctcca
     1381 aggcgctcgc ccgctcggtc tcggcgagcg cccgccggcg gtcgaggagc aggcctccga
     1441 ggctgaccag cgccagcaat gcgtgttcgc cccgggcgtc gacgcggccc ggctgatagc
     1501 ggatcagagc caccccgccg gccaacggca ccgcggccga cgcctcctcg gctcccaccg
     1561 gacgcaccag ctccaggcgc ccgtcgcgca ccagggcgcc aacggccgac ccgtgctcgg
     1621 cggcccaacg ggcgtccttc agggggtcgc gcgccggctc gcggccggcc agccgggtca
     1681 gcccggtcgg gtcgccggcc aggaccgtga actcgagcac gccgggcgtc tccgcggcgc
     1741 gatcgcagag cagctggagg gcggcggcca cgtcctggcc gcgcagggct tcgacggcca
     1801 cctggtagag cgtccccgac tccagcgcca gggactccgc ctgaactcgc gcctcggcca
     1861 ggccggagac caggcggccg ccggcgacgg ccgccagcag cagcaccagg aggtcgagga
     1921 ggtcgtgggg cgccgagatc agcagcgtcc cgtagggcgg cacgaagaag aactcgtatg
     1981 tgaggaccgc caggatcgcg gtggccaggg ccgggagcag gcggtggcgg gcgcccagcc
     2041 agatcaccag cagcaggtag aggacgccca ggttggggat gtccacccag ggccgcagga
     2101 gcccgattcc cgcggatatc agtgccacgc cggcggcgcc cagcaggtac ggcaccgcac
     2161 ggccggcggc tgctctcacc gggtgaacat cccacattca ttcgaagtcc atacggcttt
     2221 gggagttctc ctcacgggct ccccacgcct t
//
LOCUS       DASWGG010000453         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2550397, whole genome shotgun sequence.
ACCESSION   DASWGG010000453 DASWGG010000000
VERSION     DASWGG010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550397"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="VF954_08315"
     CDS             complement(<1..1457)
                     /locus_tag="VF954_08315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX9531127.1"
                     /translation="MTPPEAPLKAPDKGPEAPLKAPDKGKAGTGTPWSEFAVLVRRKS
                     HIRPIHDALRARGIPVDVVGLAGLLEVPEVLDTIAWLRVLADPGPMANRWLARILLGP
                     RFRIHYRDLTRLARWAAGQTKELTEGRRAHQPGEDGPAEVEAEPDEVAYSLSEALDHL
                     GAIEGLGPLALARLERARDEIAALRARSGEPLADLVQAVMRESGTAEALETAGPGASV
                     NLTGFLGVVAGFAPVAGDPSLPAFLAYLDAAEEVEETLEVGALSAVDSVKLMTVHQAK
                     GLEFDVVFVPGVAARANEKNERVDSIFPDERTSNPMTSQGQLPHLVREDAAHLPNPWQ
                     PDGRARKKAELQRELKERAVEDERRLFYVALTRARKRLYVTAAWWYHRQNKPRGPSVF
                     FDEVRDDPASEELAPAAMPQESPLLARLSELAVWPPDPPNRLRRDGLFPDGYPAALEE
                     LLAGAVTPEEHLARLEPAARVRAEELLAVHRGVAAA"
BASE COUNT          196 a          557 c          546 g          219 t
ORIGIN      
        1 gcggccgcta ccccacggtg gaccgcgagc agctcctccg cccggacacg ggcggcaggt
       61 tcgagacgag cgaggtgctc ctcgggcgtc acagctccgg cgagcaattc ctccagcgcg
      121 gccgggtacc cgtccgggaa cagaccgtca cgcctgagcc ggttgggcgg gtccggcggc
      181 cagacggcca gctcgctcag gcgtgcgagc agcgggctct cctgcggcat cgccgctggc
      241 gcgagctcct cgctagcggg gtcgtccctg acctcgtcga agaacaccga tggcccccga
      301 ggcttgttct ggcggtggta ccaccaggcg gcggtgacgt acaggcgttt gcgagcgcgg
      361 gtgagggcga cgtagaacag ccggcgctcg tcctcgaccg cccgctcctt gagctcacgc
      421 tgcagctcgg ccttcttccg ggcccggccg tcgggctgcc acgggttggg cagatgggcg
      481 gcgtcctccc ggaccaggtg cgggagctgc ccctgcgacg tcatcgggtt ggacgtccgc
      541 tcgtccggga agatcgagtc cacccgctca tttttttcgt tggcgcgcgc cgcgaccccc
      601 gggacgaaga cgacatcgaa ctccaggccc ttcgcctggt gcacggtcat gagcttgacc
      661 gagtcgaccg cgctgagggc accgacctcc agggtctcct cgacctcctc ggcggcgtcg
      721 agataggcga ggaacgccgg cagtgacggg tccccggcca ccggggcgaa tcccgcgacg
      781 acaccgagga agccggtcag gttgaccgat gccccgggtc ctgccgtctc cagggcctcg
      841 gcggtcccgg actcccgcat caccgcctgc accaggtccg ccagcggctc cccgctccgg
      901 gcacgcaggg cggcaatctc gtcgcgggcc cgctcgaggc gggcgagcgc cagagggccg
      961 aggccctcga tcgcgcccag gtggtccagg gcctcggaca gcgagtaggc gacctcgtcg
     1021 ggctcggcct ccacctccgc cggcccatcc tcccccggtt ggtgcgcccg ccgcccctcg
     1081 gtgagctctt tggtctgccc ggccgcccat ctggccaggc gggtcaggtc gcggtagtgg
     1141 atgcggaagc gggggccgag caggatccgg gcgagccacc ggttcgccat gggaccaggg
     1201 tcggccagga cccgcagcca ggcgatcgtg tcgaggacct cggggacctc gagcagcccg
     1261 gccaggccga cgacgtcgac ggggatcccc cgtgcccgca gcgcatcgtg gatggggcgg
     1321 atgtgggact tgcgccgtac caggaccgcg aactcggacc acggggtccc ggtgccggcc
     1381 tttcctttat ccggcgcctt gaggggcgcc tcgggtcctt tatccggcgc cttgaggggc
     1441 gcctcgggcg gggtcatgtg ccccagcttc gccgtgaccc gtgacgagca gcactcgacc
     1501 cgcggccgcg cccgcctg
//
LOCUS       DASWGG010000454         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2552658, whole genome shotgun sequence.
ACCESSION   DASWGG010000454 DASWGG010000000
VERSION     DASWGG010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2552658"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VF954_08320"
     CDS             <1..196
                     /locus_tag="VF954_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531128.1"
                     /translation="AQCVAQADAAGAARLGTLDLHATVRWQDQPAEVLSFSAPPGAGL
                     RHHLFVMALAGCGLLAVQSF"
     gene            complement(150..527)
                     /locus_tag="VF954_08325"
     CDS             complement(150..527)
                     /locus_tag="VF954_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531129.1"
                     /translation="MRPVLAVVAAAAAAAIGALVLGEYSMSGLTPVVAGLLFGFGVAE
                     VLLAAAPRPFPGPAPWTVAAWAAAAAVLTDAGLGWAAWIQLRHRHQGVPGGVWLALAI
                     GAAAGAAWVRSSGRRGARSRPTP"
     gene            553..930
                     /locus_tag="VF954_08330"
     CDS             553..930
                     /locus_tag="VF954_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531130.1"
                     /translation="MATPGAPPAPSTDRTSADWTVDVAERIETVVTTVRDRTVGPLTT
                     VARALVYGLLAGVLGVVLTVLVVIGAVRALDVYVFDRGTRTGGPHVWAVDAVVGGILT
                     LAGLLAWSRRKPRPRRGAPRRGA"
     gene            984..1967
                     /gene="trxB"
                     /locus_tag="VF954_08335"
     CDS             984..1967
                     /gene="trxB"
                     /locus_tag="VF954_08335"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX9531131.1"
                     /translation="MDQDRNVIIIGSGPAGLTAAVYTARANLHPLVIEGEPSSTSDQP
                     GGQLMLTTDVENYPGFDDGIMGPELMARFRAQAARFGAELVTAKADSVDLSGPPHAVT
                     VEGTTYTSKAVIVATGARSLMLDLPNERRLLGHGVSTCATCDGFFFKGHPIAVVGGGD
                     SALEEALFLTKFASKVTVIHRRKELRASKIMQDRAMRNERIEFLWDSVVLDVIGDASV
                     EGLHVRNVMTDDVSVLPVGGLFVAIGHVPNTSLFKGWLEMDDTGYLETRPGSTFTGRE
                     GVFACGDVQDHVYRQAVTAAGSGCMAAIDVERWLEAHHDEPDEMVATPRDW"
     gene            2021..2347
                     /gene="trxA"
                     /locus_tag="VF954_08340"
     CDS             2021..2347
                     /gene="trxA"
                     /locus_tag="VF954_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9531132.1"
                     /translation="MAEAMLTLTDSSFDEQIKSATAPVLVDFWAEWCGPCKMIAPVLE
                     EIAKEHAGRITIAKVNVDENLDIARRFEVMSIPTLILFRDGEAQTRIIGAKGKGQLLQ
                     DLEAFI"
     gene            2453..3508
                     /locus_tag="VF954_08345"
     CDS             2453..3508
                     /locus_tag="VF954_08345"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX9531133.1"
                     /translation="MTEPVVGALPLQPGDTGPAVADLQARLLALGHPLLAGRDAPVGR
                     DAGAAAGAAGCYGTETESAVRAFQRDRGLRADGVCGPQTWAVLVEAGYRLGDRLLYRR
                     APMLRGDDVGELQRRMGGLGFDAGRVDGIFGDATAAALVEFQRNVALTVDGICGPATV
                     GALRRLGSTQGGAGRGGGSDVVAGVRERERLRHGPRTLVGRRIGLGHGGEMGALANAC
                     GRALRDLGAEAVVVAHPDGSQQAHTANASEATAFVSVVLAPGQDGVASAYYRGFRYES
                     PGGRHLAQLLQVHLPPAVGVADLGTRGMAVPILRETRMPAVVCELGPPAAIVEGTASL
                     AAALGQAVAAWAAETWE"
BASE COUNT          525 a         1402 c         1344 g          496 t
ORIGIN      
        1 ggcgcagtgc gtggcccagg ccgacgccgc gggcgcggcc cggctgggca ccctcgacct
       61 ccacgccacc gtccgctggc aggaccagcc cgccgaggtg ctctccttct ccgcgccgcc
      121 gggggcgggg ctccggcatc acctgttcgt catggcgttg gccggctgcg ggctcctcgc
      181 cgtccagagc ttctgaccca tgccgcgccg gccgccgcgc cgatcgccag cgccagccac
      241 acgccgcccg ggacgccttg atggcggtgg cgcagctgga tccacgccgc ccaccccagc
      301 ccggcgtcgg tgagcacggc ggcggcggcg gcccaggcgg cgaccgtcca gggggccggg
      361 ccggggaagg gccggggggc cgccgccagc aggacctcgg ccacgccgaa gccgaacagc
      421 agcccggcca ccaccggcgt gagcccgctc atcgagtact ccccgaggac cagggcaccg
      481 atcgcagccg cagcagcggc cgccaccaca gcgaggaccg ggcgcatggg gctctgaggg
      541 tagccttccg gcatggcaac ccccggcgcc cccccggcgc cgtccacaga ccggaccagc
      601 gccgactgga ccgtcgacgt cgccgagcgc atcgagacgg tcgtcacgac cgtccgggac
      661 cggaccgtcg gaccgctcac caccgtggcc cgggcgctgg tgtacggact gctcgccggc
      721 gtgctcggcg tcgtgctcac ggtgctggtc gtgatcggcg cggtgcgggc cctcgacgtc
      781 tatgtcttcg accgggggac gcgcacgggc gggccccacg tatgggcggt cgacgcggtg
      841 gtggggggaa tcctgaccct cgccgggttg ttggcctggt cgaggcgcaa acctcgcccc
      901 cgccgggggg ctcctcgccg gggggcatag cccggtcggg cttccgccga ggacacccgc
      961 caaggaaacg cgccaaggac tccatggacc aggatcgcaa cgtaatcatc atcggctccg
     1021 ggccggccgg cctgaccgcc gcggtctaca ccgcccgggc caacctccac ccgctggtca
     1081 tcgaggggga gccgtcctcc accagcgacc agcccggcgg ccagctcatg ctcaccaccg
     1141 acgtggagaa ctaccccggc ttcgacgacg ggatcatggg gccggagctg atggccaggt
     1201 tccgggccca ggcggcccgg ttcggggccg agctcgtgac ggccaaggcc gacagcgtgg
     1261 acctgagcgg cccgccccac gcggtcaccg tcgagggcac gacctacacg agcaaggccg
     1321 tcatcgtcgc caccggcgcc cgctcgctga tgctggacct ccccaacgag cggcggctgc
     1381 tcggccacgg cgtgagcacg tgcgccacct gtgacggctt cttcttcaag ggccacccga
     1441 tagcggtcgt gggaggtggt gactccgcct tggaggaggc cctcttcctg accaagttcg
     1501 cctccaaggt caccgtcatc caccgccgca aggagctgcg ggcctccaag atcatgcagg
     1561 accgggccat gcgcaacgaa cgcatcgagt tcctgtggga cagcgtcgtg ctcgacgtca
     1621 tcggagacgc gtccgtcgag ggcctgcacg tccgcaacgt gatgaccgac gacgtgtcgg
     1681 tattgccggt cgggggactg ttcgtggcca tcgggcacgt gcccaacacc agcctgttca
     1741 aggggtggct ggagatggac gacaccggct acctcgagac caggccgggc agcacgttca
     1801 ccggccggga gggcgttttc gcctgcggcg acgtccagga ccacgtctac cgccaggccg
     1861 tgacggccgc cggctcgggc tgcatggccg ccatcgacgt ggagcgctgg ctcgaggccc
     1921 accacgacga gcccgacgag atggtcgcca caccgcgcga ctggtgaccg cccgcaaccg
     1981 gcatttgaac gacccggtgt ttcaacgaca aggagagcag gtggccgagg ccatgttgac
     2041 cctgacagac agcagcttcg acgagcagat caagtccgcg accgcaccgg tgctcgtcga
     2101 cttctgggcc gagtggtgcg ggccgtgcaa gatgatcgcg cccgtgctcg aggagatcgc
     2161 caaggagcac gccgggcgca tcaccatcgc caaggtgaac gtggacgaga acctggacat
     2221 cgcccgccgc ttcgaggtca tgagcatccc gacgctgatc ctcttccggg acggcgaggc
     2281 ccagacccgg atcatcggcg ccaagggcaa gggccagctc ctccaggacc tcgaagcttt
     2341 catctgaggt cctcggctta gcctcgtccc attacctttc caccctccgg gccggggcgt
     2401 gccagcatgg tcgcccgttg aagctcgccc gtttgaccga acctgtggcc cgttgaccga
     2461 acctgtcgtg ggcgcgctgc ccctccagcc gggggacacg ggcccggccg tggccgacct
     2521 ccaggcccgg ctgttggcgc tcggccatcc ccttctggcg ggcagggatg ctccggttgg
     2581 gagggatgct ggggcggccg caggtgcggc gggttgctac gggaccgaga ccgagtcggc
     2641 cgtgcgggcc ttccagcgag accgcggcct gcgcgccgat ggggtctgcg ggccccagac
     2701 ctgggccgtg ctcgtcgaag ccggctaccg gctcggcgac cgcctgctct accggcgagc
     2761 cccgatgttg cggggcgacg acgtgggcga gctccagcgc cggatgggag gcctcggctt
     2821 cgacgccggg cgagtggacg gcatcttcgg cgacgccact gcggccgccc tggtcgagtt
     2881 ccagcgcaac gtcgccctca cggtggatgg gatctgcggt ccggccaccg tgggcgccct
     2941 ccgccggttg ggcagcaccc agggcggcgc cgggcgaggc ggcggctccg acgtggtggc
     3001 cggggtccgg gagcgcgagc ggctccggca cggtccccgc acgctggtcg gccggcgcat
     3061 cgggctgggc cacggcgggg agatgggcgc cctggccaac gcctgcggcc gggccttgcg
     3121 cgacctcggg gccgaggcgg tcgtggtggc gcaccccgac ggttcgcagc aggcccacac
     3181 ggccaacgcg agcgaggcga cggccttcgt gagcgtggtg ctcgcacccg gccaggacgg
     3241 cgtcgccagc gcctactacc gcggcttccg ctacgagtcg cccggagggc gccacctcgc
     3301 ccagctgctc caggtccacc tcccgccggc cgtgggggtg gccgacctcg ggacgcgcgg
     3361 catggccgtg cccatcctgc gcgagacccg aatgcccgcg gtggtgtgcg agctggggcc
     3421 gccggcagcc atcgtggagg gcaccgcctc cctggccgcc gcgctcgggc aggccgtggc
     3481 tgcatgggcg gccgagacct gggagtgaga ttcccaccct gggtgccatc ccggccctgg
     3541 gtgccatccc ggccctgggt gctacccggc cctggggtgg cccgccagct ggggtggccc
     3601 gccgcctggg gtggcccacc attcttcaag caaaaagtgc gccccagcga caataaagcc
     3661 ttgaagaatg acccgattcg gtgcgacggt gccggctccc gccacccagc gccccgccac
     3721 ccagcgccct cgccacccag cgccccgcca cccgccgccc tccccgt
//
LOCUS       DASWGG010000455         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2555999, whole genome shotgun sequence.
ACCESSION   DASWGG010000455 DASWGG010000000
VERSION     DASWGG010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2555999"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="VF954_08350"
     CDS             complement(<1..1384)
                     /locus_tag="VF954_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX9531134.1"
                     /translation="MTGVLCCSITARGAELARRLPYPHHERPGGGLVAAVRGAWGEVE
                     ALVLICAAGIAVRAVAPLLGSKAEDPAVVVVDDQGRWAVPLVGGHARGANALAREIAS
                     LLGAEAVVTTAADGAGLVALDQLPGFTASGDVAGVTRRWLDGHAPCLAMDLDLRAWPL
                     PTSVTFSPQHDDSRVLVTDRDVPAAAGEVVLRPRSLVVGVGASTGAGPAGMCELVLGA
                     LAAAGLHPAAVGAVATLDRKAAEPAVVELAAALGVEVRPFAAEVLAAVDTPTPSEVVR
                     DAVGTPSVAEAAALAAAGLGSALVVAKQKAADATVAVARRARPEGSLHVVGLGPGDPA
                     LRTAAAAAVVRHAEVVVGYGPYVDLAADLLSEGQDVLRFPIGEERPRCAVALQQASEG
                     RAVALVCSGDPGVYAMAGLVLEMAGQHGLPPVSVVPGVTAALAAAAVLGAPLGHDHAS
                     VSLSDLLTPWD"
     gene            complement(1381..2598)
                     /gene="cbiE"
                     /locus_tag="VF954_08355"
     CDS             complement(1381..2598)
                     /gene="cbiE"
                     /locus_tag="VF954_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HEX9531135.1"
                     /translation="MADPPIDIVPPIDIVGLHGGQPWGPAARSAIAGAEVLAGAERHL
                     VCVASAASGSSAERVPWRGAPEEMMDQLAGLRDKGERVCLLASGDPGFFGIAELAVAR
                     FGPGGVRVHPAPSSVALAFARVPTRWDDAVVVSAHGRPLGAAVAAVLSSPKVAVLTSP
                     DLPPEALGRALVDSGCGERVVHVCSRLGEPDEAVVVCGVEELAGGSFDGLSVVIARVP
                     SGAGDGRGALWGRPEAEYLHRDGMITKAEVRAVALGKLRLPAAGVLWDVGAGSGSVAI
                     ECAALAPGLSVHAVERNPDDAARIEANAAGRRVKVVVGEAPAVLAGLPAPDRVFVGGG
                     GMPVVEAALRRLRPDGTVVATWASVARAAEAAERLGNLVQLSVSRGTRLGPDGSFRLA
                     AENPVFVTWGPQP"
     gene            complement(2591..>3094)
                     /locus_tag="VF954_08360"
     CDS             complement(2591..>3094)
                     /locus_tag="VF954_08360"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: protein motif:HMM:NF014004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HEX9531136.1"
                     /translation="VQQVDVAAAQGQRQIVLSTGGRTDAAAQRLLPSLPPVCFVEVGD
                     FTGIALRRAASRGIGEATFVGMAGKIAKLAAGVLMTHYRRSRVDTTLLAEVAATAHAP
                     AAVLAAATATATARHFAEACASAAAYEPLAELCRRAAAACTAHAGGLPVRVVMVDFEG
                     EEVLARG"
BASE COUNT          431 a         1218 c         1135 g          310 t
ORIGIN      
        1 cgtcccacgg cgtcagcagg tcggagagcg acaccgacgc gtggtcgtgg cctagcggcg
       61 ccccgagcac cgccgccgcc gccagggccg cggtcacacc gggaacgaca gagaccggcg
      121 gcaggccatg ctggccggcc atctccagca ccaggccggc catggcgtac acaccggggt
      181 cgccggagca caccagggcg accgcccggc cctcgctggc ctgctggagc gcgaccgcgc
      241 acctcggccg ctcctcgccg atggggaagc gcaggacgtc ctggccctcg ctcagcagat
      301 cggcggccag gtcgacgtac ggtccatagc ccacgaccac ttcggcgtgc cgaaccacag
      361 ccgccgcggc tgccgtccgc agcgccggat cgcccggccc caatccgacg acgtgcaggg
      421 atccctcggg ccgggcccgt cgggctactg ccaccgtggc gtcggcggcc ttctgcttgg
      481 cgaccacgag cgccgagcca agaccggcgg cggcgagagc ggcggcctcg gcgaccgagg
      541 gcgtgccgac ggcgtcgcgc acgacttcgc tcggagtcgg cgtgtccact gcggccagga
      601 cctcggccgc gaagggccgc acctccacgc cgagcgccgc ggccagctcc acgacggcgg
      661 gctccgccgc cttgcgatcg agggtggcca cggcacccac cgcggcagga tgcaaccccg
      721 ctgcggccag ggcgccgagc acgagctcgc acatgccggc cggacccgcc ccggtgctgg
      781 caccgacgcc cacgaccagc gagcggggcc gcagcacgac ctcccccgca gccgccggca
      841 cgtcgcggtc ggtcaccagg acccggctgt cgtcatgttg gggagaaaag gtgaccgagg
      901 tgggcagcgg ccaggcgcgc agatcgaggt ccatggccag gcagggagcg tggccgtcga
      961 gccatcgccg ggtcaccccg gccacgtcgc cgctcgcggt gaagccgggg agctggtcga
     1021 gggcgaccag cccggcgccg tcggccgcag tggtgacgac ggcctcggcg ccgaggagcg
     1081 aggcgatctc ccgggctagg gcgttggccc cgcgggcgtg gccgccgaca agggggacgg
     1141 cccagcggcc ctggtcgtcg accaccacca ccgccgggtc ctcggccttg gagccgagca
     1201 agggggccac cgctcgtact gcgatgccag ccgcgcagat caacaccaac gcctcgacct
     1261 ccccccacgc cccgcgcacg gccgccacca gcccgccgcc cggccgctcg tggtgcgggt
     1321 acgggagccg gcgggccagc tcggcgcccc gggcggtgat ggagcagcac agcacgccgg
     1381 tcacggctgc ggcccccagg tgacgaacac cgggttctcg gccgccagcc tgaagcttcc
     1441 gtccggaccc aagcgggtgc cgcggctgac cgagagctgg accaggttcc ccaaccgctc
     1501 ggcggcctcc gcggcccggg cgaccgacgc ccacgtggcc accaccgtgc cgtcaggccg
     1561 gagccggcgc agcgcggcct ccacgaccgg catgccccca cctccgacga acacgcgatc
     1621 cggtgccggc aggcccgcca gcacggccgg cgcctcgccc acgaccacct tgacccggcg
     1681 accggccgcg ttggcttcga tccgggcggc gtcgtcgggg ttacgttcga cggcgtgcac
     1741 ggacagaccg ggcgccagcg ccgcgcactc gatcgccacc gatcccgacc cggcgcccac
     1801 gtcccacagc acgccggctg cgggcaggcg cagcttgccg agggcgacgg cgcgcacctc
     1861 ggccttggtg atcatgccgt cgcggtgcag gtattcggct tccgggcgac cccacagggc
     1921 gccgcgcccg tcgcccgctc cggaagggac ccgggcgatc accaccgaca gcccgtcgaa
     1981 cgatcccccc gccagctcct cgaccccaca cacgaccacg gcctcgtcgg gctcgccgag
     2041 gcggctgcag acgtgcacta cccgctcccc gcatccgctg tcgaccagcg cccgccccag
     2101 cgcctcgggg gggaggtcgg gcgaggtgag cacggcgacc ttgggcgacg agagcaccgc
     2161 ggccactgcg gcgccgaggg gtcgtccgtg ggcggacacg accaccgcgt cgtcccaccg
     2221 tgtgggtacc cgggcaaacg ccagcgcgac cgacgagggc gccgggtgga cccgcacgcc
     2281 gcccgggccg aaccgggcca cggccagctc ggcgatgccg aagaacccgg ggtcgcccga
     2341 tgcgagcagg cacacccgct cccctttgtc ccgaagcccg gccagctggt ccatcatctc
     2401 ctcgggcgct ccccgccagg ggacccgctc agccgacgag cccgacgcag ccgacgccac
     2461 gcacaccagg tggcgctccg ctccggcgag cacctcggcc ccggcgatgg ccgaccgggc
     2521 cgccgggccc cacggctggc cgccgtgcag gccgacgatg tcgatgggcg ggacgatgtc
     2581 gatgggcgga tcagccacgg gcgagcacct cctccccctc gaagtcgacc atgaccacgc
     2641 gcaccgggag gccgccggcg tgggcggtgc aggcggcggc ggcccggcgg cacagctcgg
     2701 cgagtggctc gtaggcggcc gcggaggcac acgcctcggc gaagtggcgg gcggtggccg
     2761 tggcggtggc ggcggcgagc accgccgccg gggcgtgggc ggtcgccgcg acctcggcga
     2821 gcagggtggt gtcgacgcgg ctgcgccggt agtgcgtcat gagcacgccg gcggccagct
     2881 tggcgatctt gccggccatg ccgacgaagg tggcttcgcc gatgccgcgg ctggcggcgc
     2941 ggcgcaatgc gatgccggtg aagtcgccca cctcgacgaa gcacacgggc ggcagggagg
     3001 gcagcaggcg ctgggccgcg gcatcggtgc gaccgcccgt ggacagcacg atctgacgtt
     3061 ggccctgggc cgcggccacg tcgacctgtt gcac
//
LOCUS       DASWGG010000456         6207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2556467, whole genome shotgun sequence.
ACCESSION   DASWGG010000456 DASWGG010000000
VERSION     DASWGG010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2556467"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VF954_08365"
     CDS             complement(<1..988)
                     /locus_tag="VF954_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX9531137.1"
                     /translation="MARDLAIDLGTANTLVYTRGRGIVLNEPTVIALNSRTQDVLAMG
                     QEAWQMIGRTPGYIVAVRPLRKGAITDFDITQRMIRLLLQRAGVSRFNRPRVLICVPS
                     AITEVERRAVEEAARRAGAAGAFLIEQPMAAAIGAGLPIHEPLGNMIVDVGGGTTETA
                     VISLGGVVALEALRVGSFDIDNAIQIYVRREYGIAIGERTAEEIKVAIGSAYPLPDES
                     KVEVRGRDLMTGLPKTVILSPDEVRDAIEEQVGAIVDSVVKCLGKAPPELAQDLIVQG
                     IHLVGGGGLLRGMDRRLSEETAIPVHLVDAPLECVVLGAGKCLEAFDSLKVMF"
     gene            complement(981..1166)
                     /locus_tag="VF954_08370"
     CDS             complement(981..1166)
                     /locus_tag="VF954_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531138.1"
                     /translation="MGNLLYPMVAVGLSLAGALVLWLRSRKPRTLESGIEEFTRELRA
                     LAPDNGAAGHEGDGRHG"
     gene            complement(1168..1638)
                     /locus_tag="VF954_08375"
     CDS             complement(1168..1638)
                     /locus_tag="VF954_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX9531139.1"
                     /translation="MKPPATGDDWLALTTRPLPVAAAHDWAVRPGCGGIVVFSGTVRD
                     HADGRAGVTLLEYEAYEGQVVPRLAAIASGIRSRWSTVGRVVLLHRTGPLEVTEVSVV
                     VAVSAAHRGEAFSAARFGIDTLKETVPIWKRETWAGGQGWGTGASDVTGVGSRD"
     gene            1709..3025
                     /locus_tag="VF954_08380"
     CDS             1709..3025
                     /locus_tag="VF954_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX9531140.1"
                     /translation="MTVDGEHPEGGRGEQNPGGPVPDPDDRLWRHPSEIGRSATSFVA
                     AAGRARARAGRVPDTRVVAVALLGGVIGTLLGVGVMAWSRGTGSPAPSSPDGAPTQPQ
                     LLAGATSLGTGLSGLVAKASPSVIGLRVAGGGGADEAGSAVVVGPGNLVLTARRLLRS
                     RAVVTAQLAGGREEPATVVGEDVDSGLALLRVGGPPLTPLVLATSAVVNAGDTAVLVG
                     RLRGAGPGQSPTTSVLMASTAAVARTRSAAPAEVPAHVVVGEIRSVDGDPTLSGVPVL
                     GTIEADMLLDDDDIGGAVMDGRGELVGVTVASDDGNGGSSLAAPADIAQAVVAELASG
                     GRVTRGYLGVEGGDAGADIARMLGLTGGAILTQVGSDSPAAAAGVRVGDVVTEVDGRD
                     ITNMARLRALLRMHHPGDTVRLVLSRRDETRTVQVVLANPQGGSGS"
     gene            complement(3044..4069)
                     /locus_tag="VF954_08385"
     CDS             complement(3044..4069)
                     /locus_tag="VF954_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9531141.1"
                     /translation="MRTVAVTGVSGSLGQRVLAQLAAAAEVGGVVGLDRATPPEWPAK
                     LRFHPLDLATADLAPRLEGVDWVAHLAWSEAGPNLEATRRLLDAASTAGVGHLVHLSC
                     ATVYGAWPDNPVPIAEDGALRPNPQFREGVEKAEAERMVAEWAVQHPAVKVTILRPAV
                     VLGEGSPGPLARALFPVLTIRGRGEGPPIQFVDADDVAAAVVGSLVRGAEGVFNVAPD
                     GWIGDELLRSLVGPPARLPLSRRVAERAAAAVHWLRGGKAPRHAAAFGRHPLVVANDR
                     LRATGWSPRHTNEEALVASPLAGRWPELSPKRRQEVALGLAAGVAASLVAGALGLARR
                     ARRRRAS"
     gene            4087..5310
                     /locus_tag="VF954_08390"
     CDS             4087..5310
                     /locus_tag="VF954_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX9531142.1"
                     /translation="MSTPSPFGGGNPFGELFRQMSKLFTGTGPVNWEIARQVAASQEA
                     EGGGDGEGGANVDPLERIRLEELIRVAELHVAEATGLEVSQTGRALSVRAVSRTEWAW
                     ATLEAWKPLLESLGLALTPSAPSPDETAASGTPDPLGALGGGDLSGLIGGWAKALPPV
                     FLGMQAGSMVGMLAQRVLGQYDLPIPRPRSDELLVVASNLAGFASDWSLPPDDVRLWV
                     CLSEVTHHAVTGRPHVRERLDALLGAFVAGFRPDTAALEARMSQLDLTDMEAIAQALG
                     DPSAILGDMQTPEQRATLQQLEAVTAALEGYVDQVMDRVGHRLISSYPSLTEALRRRR
                     AERGEGERMVGRMLGMELPQGLFDRGSAFAAGVVERAGEGGLARLWADARALPTPAEV
                     DAPGLWLERIDLPAP"
     gene            5360..>6207
                     /locus_tag="VF954_08395"
     CDS             5360..>6207
                     /locus_tag="VF954_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531143.1"
                     /translation="MDDITFMPDLSNVLGQVYAHLDEQAIPPAVAPAPLQPAPAGGPT
                     TPEWADDAHLEQAFANWSPGTPPEASAAERRFAANLPAHREPAAPHVPIDAPARPVDD
                     ELAAALNAAVAAAPDHPVDNDHPADNQASPDPGAVPAPVAAPAYGPGPESTPTVYPPL
                     SAPPVAAPPVAATPLPATPVPVGPQPKDDYAFAWIDPQVPVEAPIEGVGARPDGPAAE
                     APVAYRPWQRTDDDILPSKGGRARKSRNAVPGARPEPMIASAMVPMIAPATVPAAASA
                     TVPMAAS"
BASE COUNT          807 a         2336 c         2277 g          787 t
ORIGIN      
        1 tgaacatcac cttcagcgag tcgaaggcct ccaggcactt ccccgccccc agcaccacgc
       61 attcgagggg cgcgtccacc aggtgcacgg gaatggcggt ctcctccgag aggcgtcggt
      121 ccatgccgcg cagcaggccc ccgccgccga cgaggtggat gccctgcacg atcaggtcct
      181 gggccagctc cgggggcgcc ttgcccaggc acttcaccac ggagtcgacg atggcgccga
      241 cctgctcctc gatggcgtcg cgcacctcgt cgggagaaag gatcacggtc ttgggcaggc
      301 cggtcatgag gtcccggccc cggacctcga ccttgctctc gtcgggcagc gggtaggccg
      361 acccgatggc gaccttgatc tcctccgcgg tgcgctcgcc tatggcgatg ccgtactcgc
      421 gacggacgta gatctggatg gcgttgtcga tgtcgaagct gccgacccgc agggcctcga
      481 gggcgaccac gccgccgaga gagatgaccg ccgtctccgt ggtgccaccc ccgacgtcga
      541 cgatcatgtt cccgaggggc tcgtggatcg gcaggccggc gccgatggcc gcggccatgg
      601 gctgctcgat gaggaaggcg ccggccgcgc ccgcccgccg ggcggcctcc tcgaccgccc
      661 tgcgctccac ctcggtgatg gccgacggca cgcagatgag gacccgcggc cggttgaacc
      721 gcgacacccc cgcccgctgc aacagcagcc ggatcatgcg ctgggtgatg tcgaagtcgg
      781 tgatggcgcc cttgcgcagg ggccgcacgg ccacgatgta gccaggcgtc cggccgatca
      841 tctgccatgc ctcctggccc atggcgagca catcctgggt gcgcgagttc aaggcgatga
      901 cggtgggctc gttgagcacg atgccacgcc cccgggtgta caccagggtg ttggccgtcc
      961 ccaggtcgat ggccaggtcc ctagccatgc cggccgtcgc cctcgtggcc ggcggccccg
     1021 ttgtcgggcg cgagggcccg cagctcgcgc gtgaactcct cgatccccga ctccaacgtc
     1081 cgcggcttgc gcgagcgcag ccacaggacc agggcaccgg caaggctgag gccgacggcg
     1141 accatcgggt agagcaggtt tcccatgtca gtcccggctt cccaccccgg tcacgtcgct
     1201 ggcgccggtg ccccagccct gcccgcccgc ccaggtctcg cgcttccaga tggggacggt
     1261 ctccttcagc gtgtcgatgc cgaagcgggc ggccgagaag gcctcgccgc ggtgcgccgc
     1321 gctgaccgcg accacgaccg acacctcggt gacctcgagg ggaccggtgc gatgcagcaa
     1381 cacgacccga ccgaccgtcg accaacggga ccggatcccg gatgcgatgg ccgccaaccg
     1441 ggggacgacc tggccctcat aggcctcgta ctcgagcagg gtgaccccgg cgcggccgtc
     1501 ggcatgatct cgcaccgtac cgctgaacac cacgatccca ccgcaccccg ggcggacggc
     1561 ccagtcatgg gcggccgcga ctgggagtgg tcgggtcgtc agggcgagcc aatcgtcgcc
     1621 tgttgccggg ggcttcactg cctttatgct acggggcggc tcggtggggc gatgctttcc
     1681 gcgcgggacc ggccgttatc ctcggttcgt gaccgttgac ggagagcacc ccgaaggcgg
     1741 ccgcggggag cagaacccgg gtggccctgt cccggacccc gacgaccgcc tgtggaggca
     1801 cccgtcggag atcgggcgat cggccacgtc gttcgtcgcc gccgccggcc gggcgcgggc
     1861 gcgcgccggc cgggtgcccg acacccgggt cgtcgcggtg gccctgttgg gcggcgtcat
     1921 cggcaccctg ctcggcgttg gcgtgatggc gtggtcccgg gggacgggca gcccggcgcc
     1981 gtccagcccg gatggtgcgc ccacccagcc ccagctgctg gccggcgcca cctccctggg
     2041 gaccggcctc agcggtctgg tcgccaaggc cagcccgtcg gtcatcggcc tgagggtcgc
     2101 cgggggcggc ggcgccgacg aagccgggtc cgcggtggtc gtcgggcccg gaaatctcgt
     2161 gctgaccgcc cgccggctcc tgcgcagccg ggcggtggtg acggcccagc tggccggtgg
     2221 gcgggaggag ccggccaccg tcgtcggcga agacgtcgac agcggcctcg ccctgcttcg
     2281 agtcggcggc ccgccgctca cgcctttggt cctggccacc tcggccgtcg tcaatgccgg
     2341 cgacacggcg gtgctggtgg gtcggctccg cggcgccggc ccggggcaga gcccgaccac
     2401 cagcgtgctc atggccagca ccgcggccgt ggcgcggacc aggagcgctg cacccgccga
     2461 ggtcccggcg cacgtggtgg tgggcgagat ccgcagcgtc gacggcgacc cgaccctttc
     2521 cggggtcccg gtgctcggca ccatcgaggc cgacatgctc ctcgacgacg acgacatcgg
     2581 cggcgcggtc atggacgggc ggggcgagct ggtcggcgtc accgtggcgt ccgacgacgg
     2641 aaacggcggg agcagcctgg ccgcgcccgc cgacatcgcc caggccgtgg tcgccgagct
     2701 ggcgtcgggc ggcagggtga cccgcggcta cctcggggtc gaaggcggcg acgcgggcgc
     2761 cgacatcgcc cggatgctgg gcctgacggg cggcgccatc ctcacccagg tgggatccga
     2821 cagtcccgcg gccgccgcag gcgtccgggt gggcgacgtc gtgaccgagg tcgacggccg
     2881 cgacatcacc aacatggccc ggctgcgggc gctgctccgc atgcaccatc ccggcgacac
     2941 cgtgcgattg gtgctgtctc gacgggacga gacccgcacc gtccaggtgg tcctcgccaa
     3001 cccgcagggc gggagcgggt cctaacgtcc cagctggcgc ggcctagctg gcgcggcgcc
     3061 ggcgggcccg ccgggccagg ccgagggcgc cggccacgag ggaggcggcc accccggcgg
     3121 ccagaccgag ggcgacctcc tggcgccgct tggggctcag ctccggccac cgccccgcca
     3181 gggggctggc cacgagggcc tcctcgttgg tgtggcgggg cgaccagccc gtggcccgca
     3241 gccggtcgtt ggccaccacc aggggatggc ggccgaaggc ggcggcgtgc cgcggcgcct
     3301 tgccaccgcg cagccagtgc acggcggccg cggcccgctc ggccacccgg cgggagaggg
     3361 gcaggcgggc gggcgggccc accaggctgc ggagcagctc gtcgccgatc cagccgtcgg
     3421 gggccacgtt gaacaccccc tccgcgcccc tgaccaggct gcccaccacg gcggccgcca
     3481 cgtcgtcggc gtccacgaac tggatcggtg ggccttcccc ccgcccgcgg atcgtgagca
     3541 ccgggaacag cgcccgggcg aggggacccg gcgatccctc cccgagcacg accgcggggc
     3601 ggaggatcgt caccttgacc gcggggtgct gcaccgccca ctcggcgacc atgcgctcag
     3661 cctcggcttt ctccaccccc tcccggaact gggggttggg ccggagggcg ccgtcctcgg
     3721 cgatgggtac ggggttgtcc ggccacgccc cgtagacggt ggcgcaggaa agatggacca
     3781 ggtggcccac gcctgcggtc gaggcggcgt cgagcaggcg gcgggtggcc tcgaggttgg
     3841 gcccggcctc ggaccaggcg agatgggcga cccagtcgac tccctcgagc cggggcgcca
     3901 ggtccgccgt ggccagatcg agcgggtgaa agcggagctt ggccggccac tcgggcgggg
     3961 tggcccgatc caggccgacg actccaccca cctcggcggc ggctgcgagc tgggccagga
     4021 cgcgctggcc caggctgccg gaaaccccgg tgacggcaac agtccgcacg ggcctaccat
     4081 gccgctgtga gcactccatc gccattcggc gggggcaatc ccttcgggga gctgttccgc
     4141 cagatgagca agctgttcac cgggacgggg ccggtcaact gggagatcgc caggcaggtg
     4201 gccgcctcac aggaggcgga aggcgggggc gacggtgagg gtggggccaa cgtcgacccg
     4261 ctggagcgca tccggctgga ggagctgatc cgggtcgcgg agctgcacgt cgcagaggcc
     4321 accgggctcg aggtctcaca gacgggccgg gcgctctccg tccgcgcggt gtcgaggacg
     4381 gagtgggcgt gggcgaccct cgaggcgtgg aagcccctgc tcgaatccct cgggttggcg
     4441 ctgaccccgt cggctccgtc accggacgag acggctgcgt ccggcacccc ggacccgctc
     4501 ggcgccctcg gtggcggcga tctcagcgga ttgatcgggg gttgggcgaa ggccctgccg
     4561 ccggtgttcc tcggcatgca ggccggctcg atggtcggca tgctcgcaca gcgggtgctc
     4621 ggccagtacg acctgccgat cccgaggccg aggtccgacg agctgctggt ggtggcgtcc
     4681 aacctggcgg gcttcgcatc ggactggagc ctgcccccgg acgacgtccg gctctgggtc
     4741 tgcctcagcg aggtcacgca ccacgccgtg acgggccggc ctcacgtgcg ggagcgcctc
     4801 gatgccctcc tgggcgcgtt cgtggccggc ttccgaccgg acaccgccgc cctcgaggcg
     4861 cgcatgtccc agctggacct gaccgacatg gaggccatcg cccaggcgct gggcgacccg
     4921 agtgccatcc tcggcgacat gcagacgcct gagcagcggg ccaccctcca gcagctcgag
     4981 gcggtcacgg cggccctcga gggctacgtc gaccaggtga tggaccgcgt cgggcaccgc
     5041 ctcatctcgt cgtatccctc gcttacggag gccctccgcc ggcggcgggc cgagcggggc
     5101 gagggtgagc gcatggtggg ccggatgctg gggatggagc tgccccaggg cctgttcgac
     5161 cggggttcgg cgttcgcggc cggcgtggtc gagcgtgccg gcgagggcgg gctggcccgg
     5221 ctgtgggccg acgcccgggc gctgcccacg ccggccgagg tggacgcgcc gggcctgtgg
     5281 ctggagcgca tcgacctgcc ggcgccctag ctccgccggc ggccactccc ggtggccggt
     5341 gctcaagcag aacgcccaaa tggacgacat caccttcatg ccggatctct cgaacgtgct
     5401 gggccaggtc tacgcacacc tggacgaaca ggccattccg cccgccgtgg cacctgcacc
     5461 gctccagccg gccccggccg gcggcccgac cacgcccgag tgggccgatg acgcccacct
     5521 cgagcaggcg ttcgccaact ggtcgcccgg cacgccgccc gaggcgtccg ccgccgagcg
     5581 gcgcttcgcc gccaatctgc ccgcgcaccg cgagcccgcc gccccgcacg tcccgatcga
     5641 cgctccggcc aggcccgtgg acgacgagct ggccgccgcc ctgaacgcgg cggtggccgc
     5701 ggccccggac caccccgtgg acaacgacca ccccgctgac aatcaagcct cccctgaccc
     5761 cggcgccgta ccggccccgg tggcggcccc cgcctacggg cccgggccag aatcgacgcc
     5821 cacggtgtac ccgccccttt ctgccccccc ggtggccgcc cccccggtgg ccgccacccc
     5881 gctgcccgca accccggtgc ccgtcgggcc ccagccgaag gacgactacg cctttgcctg
     5941 gatcgacccc caagtcccgg tcgaggcccc gatcgagggc gtgggcgccc gccctgatgg
     6001 ccccgccgcc gaagcaccgg tcgcgtaccg gccgtggcag cgcaccgacg acgacatcct
     6061 cccgagcaag ggcgggcgcg cccgcaagtc gcgcaacgcc gtccctggcg cccggcccga
     6121 gccgatgatc gcctccgcga tggttccgat gatcgccccc gcgactgtgc cagcggccgc
     6181 ctcggcgact gtgccgatgg ccgcctc
//
LOCUS       DASWGG010000457         3857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiales bacterium isolate SMAG_U2809
            k141_2574212, whole genome shotgun sequence.
ACCESSION   DASWGG010000457 DASWGG010000000
VERSION     DASWGG010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960457
            Sequence Read Archive: SRR10360651
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiales bacterium (soil metagenome)
  ORGANISM  Acidimicrobiales bacterium
            Bacteria; Actinomycetota; Acidimicrobiia; Acidimicrobiales.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:12:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,681
            CDSs (total)                      :: 1,668
            Genes (coding)                    :: 1,661
            CDSs (with protein)               :: 1,661
            Genes (RNA)                       :: 13
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 11
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Acidimicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2574212"
                     /isolate="SMAG_U2809"
                     /isolation_source="soil"
                     /db_xref="taxon:2201156"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VF954_08400"
     CDS             complement(<1..721)
                     /locus_tag="VF954_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9531144.1"
                     /translation="MPTYAVANQKGGVAKTTTVHALGTAFAESGSRVLLVDLDPQASL
                     TYSAGLDPDSIERSINDVLVRRAPAADILTKAGDLHVLPSTIDLAGAEIHLLTRPGRE
                     YALARALEPLAGSYDVVLVDCPPSLGVLTINGLTAADEVLIPVQCETLSRRGVGQLLE
                     TIADVRSFTNARLRVRGAIATMFDGRTRLAREVLEDIRQRYDIDVLEPPVPKSVRVAE
                     APAGGSSVLEYAPRSPSAEAYR"
     gene            complement(891..>3857)
                     /locus_tag="VF954_08405"
     CDS             complement(891..>3857)
                     /locus_tag="VF954_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9531145.1"
                     /translation="ITEGYLVPLGLARVRRDGITVSPDARQPAVAETLRLVGAGGPVP
                     AVEVAGALAEGPLGLTQPEIILVLNACRQSGLIEAWRGRRRVTEPFLALAPTDRLGPG
                     ELVEPAVQEAVGALGPITGPGPFDPWTSSTQRGAWDYAKAWLEARSEDAAQTRRALAQ
                     AGDVALLAGADSGAVQRDLTLVETVLQGTGRAADPAEGLRHLVAALPDGVSVTEAAGR
                     IGAVARFLRDDLRRTDEELAYLTHPDLTVPADETALLEGREVALDLLGDVLGRAADGR
                     LGEVTAAVREFRSAYRAAYLDAHDRHYAAVPPGALDAIRATAAYRAVAALSSIGALAV
                     PDDRVKIDRLLAAAVPPPCRLRVDVELGWKPRCACRLALGDPPPSLDEAAVVGVAARG
                     IEQYLGELARPETQARLGEAADDLVALGQTELAADVRRLQSLASGSAADADEVVSLLQ
                     PPVARVVREVLTGGQLIVSRDLGALREDLVGRRFPKRRLAELLAAWLDATGELPGGTF
                     VEVVDGTAEKGHDRGPEPRGATAALLAERFPALAAALPEHQPADAFWVAAWWAGRSSP
                     APGWIPSRLLERGDLLVTAAVAAAALPEPRAELAELDGRTTGDSVLGEAIAAALDLTA
                     MPVADVFTVLAGERLLSHPVRLAAGELVRRLAADVAYVSAVGEVGEWGRDHALLGRPD
                     LAPLDHLVTAARHLASIERRLGAGLALPALVEDLYPSHAAPVPALVSRASLANSVCAL
                     LPADAVEAFRVAAGRVLRALDAELATHGPGDWPGCLPIWDVGQAVVAPLLGRYGRVAV
                     LLVDAMRVDLGAVLTAMIAEALPERVVRQRFAVVPTPTRTAEALAALASGRPAPGGSV
                     PSAFDQAVVPFGHLGYEASALVGADRDDQAQALRTLWSEGPPVSVAVATGVDERLHRT
                     SVDVAELLDEAAAAFSRRVLPALAAVPAAVPLVLLADHGFRQNPSWGHGPEGRYVHGG
                     PSLEESIVPVVVFEPG"
BASE COUNT          526 a         1449 c         1474 g          408 t
ORIGIN      
        1 gccggtaggc ctcggcgctg ggcgagcgcg gcgcgtactc gagcaccgac gagccgccgg
       61 ctggcgcctc ggccacgcgc acggacttgg gaacgggcgg ttcgagcacg tcgatgtcgt
      121 agcgctgccg gatgtcctcg aggacctcac gggccaggcg ggtgcgcccg tcgaacatgg
      181 tggcgatggc gccccgcacc ctcaggcgcg cgttggtgaa cgaccgcacg tcggcgatcg
      241 tctcgagcag ctggccgacg ccccggcggc tcagcgtctc gcactggacg gggatcagca
      301 cctcgtccgc cgccgtcagc ccgttgatgg tgagcacccc gagactgggc gggcagtcga
      361 ccagcacgac gtcgtaggag ccggcgagcg gctcgagggc gcgcgccagc gcgtactcac
      421 gtcccggccg ggtcagcagg tggatctcgg ccccggccag gtcgatggtc gacggcagca
      481 cgtggaggtc gcccgccttg gtcaggatgt ccgccgcggg cgctcggcgg acgagcacgt
      541 cgttgatcga gcgctcgatc gagtcgggat cgagcccggc ggagtaggtc agcgacgcct
      601 gcgggtcgag gtcgaccagc agcacccggc tcccgctctc ggcgaacgca gtgccgaggg
      661 cgtgcacggt cgtcgtcttg gccaccccac ccttctggtt ggcaaccgca taggtgggca
      721 ccgcgagatt ctaaggaccg gccgcacggc ctgcacggcc ccgccggcgg cgccagctgg
      781 agggtcgagg gcattcttca agcagaaaac acgctgggac gacattaaag cattgaagaa
      841 tggccagtgc gggccgagcc gggccgagcc gggccgatcc gggccgaggg ctatccgggc
      901 tcgaagacca cgacgggcac gatgctctcc tccaacgacg gcccgccgtg gacgtaccgg
      961 ccctcgggac cgtggcccca cgacgggttc tgccggaagc cgtggtcggc cagcaggacc
     1021 aggggcacgg ctgcggggac ggcggcgagg gccggcagga cccgccggga gaaggcggcc
     1081 gcggcctcgt ccagcagctc ggccacgtcg accgaggtgc gatggaggcg ctcgtcgaca
     1141 ccggtcgcga ccgcgacgga gacaggcggt ccctcgctcc acagggtccg cagcgcctgg
     1201 gcctggtcgt cccgatcggc gcccaccagg gcggacgctt cgtatcccag atggccgaaa
     1261 gggaccacgg cctggtcgaa cgcgctcggc accgacccgc ccggtgcggg ccggccggag
     1321 gcgagcgcgg cgagcgcctc ggccgtgcgc gtgggcgtcg ggacgacggc gaagcgctgc
     1381 cgcacgacgc gctcggggag ggcctcggcg atcatggcgg tcagcacggc gccgaggtcg
     1441 acccgcatgg cgtcgaccag caggacggcg acgcgcccgt accggccgag cagcggggcg
     1501 accacggcct ggccgacgtc ccagatcggc aggcaccccg gccagtcgcc gggcccgtgg
     1561 gtggccagct cggcgtccag ggcccgtagc acgcgcccgg ccgcgacccg gaacgcctcc
     1621 acggcgtccg ccggcagcag ggcgcagacc gagttggcca ggctggcccg gctgaccagg
     1681 gccgggaccg gcgcggcgtg cgacgggtac aggtcctcga ccagcgccgg cagggccaac
     1741 ccggcgccga ggcggcgctc gatgctggcc aggtggcggg ccgctgtgac caggtggtcg
     1801 agcggagcga ggtcgggccg gccgagcagg gcgtggtccc ggccccactc ccccacctcg
     1861 ccgacggccg acacgtaggc cacgtccgcg gccagccggc gcaccagctc gccggcggcc
     1921 agccgcacgg gatgggagag cagccgctcg ccggcgagga cggtgaacac gtccgccacg
     1981 ggcatggcgg tcaggtccag ggccgcggcg atcgcctctc ccagcacgct gtcgcccgtg
     2041 gtgcgcccgt cgagctcggc cagctcggct ctcggctcgg gcagcgcggc cgccgcaacc
     2101 gccgcagtta ccaggagatc gccccgctcg agcagccggg aggggatcca acccggcgcc
     2161 gggctggacc ggccggccca ccaggccgcc acccagaagg cgtcggccgg ctggtgctcg
     2221 ggcagcgcgg cggccagggc cgggaatcgt tccgccaaca gcgcggcggt ggcgccgcgg
     2281 ggctccggcc cgcggtcgtg tcctttctcc gccgtcccgt ccaccacctc cacgaacgtc
     2341 ccacccggca gctcaccggt ggcgtccagc caggcggcca ggagctcggc caaccggcgc
     2401 ttggggaagc ggcgccccac caggtcctcg cgcagcgccc ccaggtcccg cgacacgatg
     2461 agctggccgc cggtcagcac ctcccgcacc acgcgcgcca ccggcggctg gaggagcgac
     2521 accacctcgt cggcgtcggc cgccgagccg gacgcgaggg actgcaaccg ccgaacatcg
     2581 gccgccagct ccgtctggcc gagtgccacg aggtcgtcgg ccgcctcccc cagccgggcc
     2641 tgggtctccg gccgggccag ctcgccgagg tactgctcga tcccgcgggc cgccaccccg
     2701 accacggcgg cctcatcgag ggacggcgga gggtccccca gggccagacg gcaggcgcac
     2761 cgcggcttcc agcccagctc gacgtcgacc cgcagccggc acggaggcgg cacagcggcg
     2821 gccaggagcc ggtcgatctt gacccggtcg tcgggcacgg ccagagctcc gatggaggaa
     2881 agcgcagcca cggcgcggta ggccgccgtg gcgcggatgg cgtccagcgc gccggggggg
     2941 acggcggcgt agtggcgatc gtgggcgtcc aggtacgcgg cgcggtaggc gctgcggaac
     3001 tcccggacgg ccgcggtcac ctcgccgagc cggccatcgg ccgcccggcc gagcacgtcg
     3061 cccaacaggt cgagggcgac ctcgcgcccc tccagcagag ccgtctcgtc ggcggggacg
     3121 gtcagatcgg gatgggtcag gtaggcgagc tcctcgtcgg tccggcgcag gtcgtcgcgc
     3181 aggaaccggg ccaccgcgcc gatgcgaccg gcggcctcgg taacgctgac cccgtccggc
     3241 agggccgcga ccagatgacg gaggccctcg gccgggtcgg ccgcccgccc ggtgccctgg
     3301 aggaccgtct cgaccagggt gaggtcgcgc tggactgcac cggagtcggc cccggccagg
     3361 agggcgacgt cgccggcctg cgccagggcg cgccgggtct gggccgcgtc ctcggaccgg
     3421 gcttcgagcc acgccttggc gtagtcccac gcgccccgct gcgtgctcga cgtccacggg
     3481 tcgaacgggc cggggccggt gatgggcccg agcgctccca ccgcctcctg cacggccggc
     3541 tcgaccagct cgcccgggcc gagccgatcg gtcggcgcga gcgccaggaa cggctcggtg
     3601 acgcgccggc gcccccgcca cgcctcgatg agccccgact gccggcacgc gttgaggacc
     3661 aggatgatct caggctgggt cagcccgagc ggcccctcgg ccagggcgcc cgccacctcg
     3721 acggccggga cagggccgcc cgcgccaacc aggcgcagcg tctcggccac ggccggctgg
     3781 cgggcgtcgg ggctcacggt gatgccgtcg cggcggaccc gggccagccc gaggggcacg
     3841 agatagccct cggtgat
//
